LOCUS       DATMPU010000001         3731 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_2170662,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000001 DATMPU010000000
VERSION     DATMPU010000001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 3731)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3731)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3731
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2170662"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            460..1545
                     /locus_tag="VNY06_00005"
     CDS             460..1545
                     /locus_tag="VNY06_00005"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HWX13260.1"
                     /translation="MVKVLISAPSLDVAKNVSGISSVVMQVMHVLSPYIEFRHLELGS
                     EQGGGLFVRLAVSLSKSLRSLFTALTSSYEVFHSNTAMNPKSILRDLAMVAAARLRGK
                     PLLLHIHGGRYVHDRPTILLRYAQRLLFHFSQRIVVLSRTELSYFTQNYPETAGKTEY
                     IYNGTSVSEEAAAVDRKASALGHLRVAFVGRLAPEKGLATLISACLLLKETDEIDLDV
                     FGNGDLLPDVLALTQQKAFVKHRGIFQPAECRKILQTYDALVLPSLRGEGMPMAVIEA
                     MSAGTIPICTPISSIPEIITDGETGLLIEPGSPDAIVAALMRLKQDAPARRRMGSAAY
                     EFATANLDAEKNFGKLKGLYERLCAGS"
     gene            complement(1588..3012)
                     /locus_tag="VNY06_00010"
     CDS             complement(1588..3012)
                     /locus_tag="VNY06_00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003612245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HWX13261.1"
                     /translation="MSLKAFLRTKSVEQLQSESERPGQFRRVLGLWQLTGIGLGGIIG
                     AGIFVLTGVVAATQAGPAVSLSYVIAGIASAAAALCYAEFAGMIPAAGSAYTYGYAAL
                     GEIVAWVIGWDLLLEYALVVAVVSIGWSGYLRVLLGQFGLSLPLWAMGAAGTGAGHIL
                     DLTAMLGSLAVALLLTLRVEWGARFNTAMVVLKIAAVILVIAAGLPHVHTANWHPFMP
                     YGFGGVAEGAAVVFFAVFGYDTLTTAAEEAQDPQREVPRAVLLSLSISLVLYIAMSLV
                     LTGIVRFDKLNNAAPVASAFTAIGLPWVTLVVSAAAVAGIVSVMLAFMLGCARIWFAM
                     SRDGLLPAWFAKVHPRYRTPHRPTLIAGVLTALVAGLFPIKEIAQLVNIGTLSAFVVI
                     CFAVIVLRRTRPDARRAFHTPLVPWIPLSGVGFSIWLLSKLPSAAWYLFIIWMALGLV
                     LYLSYGIHHSVLANERSAEPPRPS"
     gene            complement(3158..>3731)
                     /locus_tag="VNY06_00015"
     CDS             complement(3158..>3731)
                     /locus_tag="VNY06_00015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HWX13262.1"
                     /translation="PFVVAFTGVRGIVSLAAALAIPFATAAGNPFPDRDLILFLTFSV
                     ILVTLVGQGLTLPLVIRALGLAHAGRRERRGVKAEERAARRQAVEAALARLDELATER
                     ALPNGVITPLRAQHRARLKHSEHHEDGNDGHKKLAELHHEIELLVIGAERRRTNELFC
                     AGKLTDEARRRIEHELDLREAQLANHRDED"
BASE COUNT          855 a         1075 c         1074 g          727 t
ORIGIN      
        1 tggcggccgg aacgggaaga cgactgtctc aaccaattta tcgcattcgc cgagcgccat
       61 tgcgtgcgcg atggtcgcgc gcacagccgc ccgtcgtcac acgccgcttg gcaccatgac
      121 ggcacacggc tgacttataa ttataccaaa agtcggtgaa ccaactcttg tcgtcctccg
      181 gtccggaatc aagtccggta ccggcgagac gagggaaatt cacgggttgt tatcaggatc
      241 ggcgtagggg ttattcgccc cgattgagct gcgccctcac attgaggtgc ccggcgaagt
      301 tgacattgcc gaggaagagc tagagcgtgt tgagccaaag gtgggctccg gttttgcgtc
      361 cggatacgcg acaaaacaaa gatttagagc atgatggcga ttcgaagaaa agtcatcttg
      421 ctcgagcttc attataagca cggcggatga ggttccgaca tggtcaaagt tctcatttca
      481 gcaccgtccc tagatgtcgc gaaaaatgtg agcggcatct cctcggtcgt catgcaggtc
      541 atgcatgttc tttcccccta tattgaattt agacatttgg agctcggatc ggagcaaggc
      601 ggcgggcttt ttgtgaggct tgccgtttcc ctttccaagt ccctgcggtc gctcttcaca
      661 gcgctgacat cgagctacga agtttttcac tcaaacacag cgatgaaccc aaagtcgatc
      721 ctccgagatt tggcgatggt cgcggcggcg cggcttcgcg gcaaacccct gttgcttcat
      781 atccacggcg gcagatatgt tcatgaccgt ccgacgattc tgctacgcta tgcgcagcgg
      841 ctcctgttcc atttctccca gcgcattgtt gtgcttagcc ggactgaact ttcctacttt
      901 acgcaaaatt accccgaaac tgcgggtaag acggaatata tttacaatgg caccagtgtg
      961 tctgaggaag ccgctgcagt tgaccgtaag gcttccgcac taggacactt aagggttgcc
     1021 tttgttggcc gcctggcgcc ggagaaaggt ctcgctacgc tcatttcagc atgcctgttg
     1081 ctcaaagaga ccgatgaaat tgacctcgat gtttttggca atggcgactt gctgcccgat
     1141 gtgctggcgc tgacgcaaca aaaggccttt gtaaagcatc gtggaatttt tcaacccgct
     1201 gaatgccgca agattttgca aacttatgat gcgctcgttc ttccgtcgct tcgaggagag
     1261 gggatgccca tggcggtcat tgaggccatg tcggcgggca ccatcccaat ttgcactcca
     1321 atctcctcaa tcccagagat catcaccgat ggcgagaccg gattgctcat cgagcccggt
     1381 tcaccggatg ccattgtcgc ggccttaatg cgtctcaagc aggacgcacc agccaggcgc
     1441 aggatgggga gcgcggctta tgaattcgcg acggcaaatc ttgatgctga gaagaatttt
     1501 ggcaagctca aaggcttata tgagaggctt tgtgctggat cctgatttaa ttcctttggt
     1561 ttcgattaac gcaattcttt ggattcatca ggaaggtcgt ggaggttcgg cgctcctttc
     1621 gtttgcgagc acgctgtgat gaatgccgta ggaaaggtaa agcaccagcc cgagcgccat
     1681 ccaaatgatg aataagtacc acgcggcgga ggggagcttt gacaagagcc aaatcgaaaa
     1741 gccgacgccg gaaagtggaa tccaaggcac gaggggcgtg tggaacgcac ggcgcgcgtc
     1801 gggcctcgtg cgccgcagca cgatgaccgc aaagcaaatg acaacaaagg cagaaagcgt
     1861 tccaatattt accaattgcg cgatctcctt gatcgggaac aatcccgcga ccaaagcggt
     1921 gagcacgccg gcgatgaggg tcgggcggtg gggggtgcga tagcgcgggt gcaccttagc
     1981 aaaccatgcc ggaaggagcc cgtcccggct catggcgaac caaatgcggg cgcaacccag
     2041 catgaaggca agcatgacac tgacaatgcc agctacagcc gccgcagata cgacgagcgt
     2101 cacccagggc aggccaatcg ccgtgaaggc ggacgcgacg ggcgcagcat tgtttaattt
     2161 gtcaaagcgc acgatgcccg tcagcaccaa agacatcgct atgtaaagca cgagcgagat
     2221 gctgagcgag agcaaaacag cgcgtggaac ctcgcgctgc ggatcctgtg cttcttcagc
     2281 cgcggtcgtg agcgtatcgt aaccgaacac tgcaaagaaa acgacagccg ccccttcggc
     2341 cacgccgcca aatccatagg gcatgaaagg gtgccaattg gcggtgtgca catggggtag
     2401 accggcagcg atgacgagga tcaccgccgc gatcttcagg accaccatcg ccgtattgaa
     2461 gcgtgcgccc cactcgacgc gcaaggtcaa tagcaaagcg accgccaggc tcccgagcat
     2521 tgcggttaga tccagaatat gaccggcgcc agtaccggcc gcacccattg cccaaagagg
     2581 gaggctaagg ccaaattggc caagcaagac ccgcaggtag cccgaccagc cgatcgagac
     2641 gacggccacg accagtgcat attccagcag caagtcccag ccgattaccc aggcgacaat
     2701 ctcgccaagc gcagcgtatc cataggtata ggcactgcca gccgcgggaa tcatcccggc
     2761 aaactcggcg tagcaaaggg ccgcggccgc gctcgctatg ccggcgatta cataggaaag
     2821 cgacacggct ggacccgcct gggttgcggc cacaacgcct gtaagcacaa agatgccagc
     2881 gccgatgatc ccgccaagcc cgataccggt gagctgccag agtccaagca cacggcggaa
     2941 ctgcccgggc cgctcgctct ccgattgcaa ttgctcgacg gacttggtcc gcaaaaatgc
     3001 cttgagagac atagatttcc aaaacgaccg cattgcaagc gccttccgcc gtctcgggtg
     3061 gcagcctata cgtaccctcg tgccgcgtca cgtagctctg gccgcagcag cagcaatcta
     3121 agctttccct attcccaaaa agctgagcgc tctgccgtta atcttcatct cgatgattgg
     3181 ccaattgcgc ctcccgcaaa tcgagttcgt gctcgatccg gcgcctcgct tcatcggtga
     3241 gcttgccggc gcagaaaagt tcattggtcc gccggcgctc ggcgccaatc accaaaagtt
     3301 cgatttcgtg atgcagctcg gcaagctttt tgtgcccgtc gttgccgtct tcatgatgct
     3361 cggaatgttt gagcctggca cgatgctggg cgcgcagcgg cgtgatgacg ccattcggaa
     3421 gggcacgctc tgtggcaagc tcgtcgagcc gggcaagcgc ggcttcgacg gcctggcgtc
     3481 tggcagcgcg ctcctcagct ttcacgccgc ggcgctcgcg ccggcctgca tgcgcaagac
     3541 caagcgcacg aatcacaaga ggcagagtga gcccctgtcc gacaagcgtt accagaatta
     3601 ccgagaatgt cagaaacaaa atcaggtcgc gatcgggaaa agggttgccc gccgcggttg
     3661 cgaatggaat ggcaagggct gccgcgagcg aaacgattcc gcgcacaccc gtaaaggcga
     3721 caacgaaggg c
//
LOCUS       DATMPU010000002         5257 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_621676,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000002 DATMPU010000000
VERSION     DATMPU010000002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 5257)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5257)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5257
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_621676"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(376..1233)
                     /locus_tag="VNY06_00020"
     CDS             complement(376..1233)
                     /locus_tag="VNY06_00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018264347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HWX13263.1"
                     /translation="MLTFSRIAGTVVLGLGLSAPAARAAAFEAADGARVEKAPLQIVE
                     KAPLQIYATPQAALRTALEGFRLGDTERAFEALKYAAEGGELLAQWKLAKIYASGDGV
                     PREDLKAYEYFSQIVSNYDEDNPDPRSAAIVSSAFVALGIYSLNGIENSEVVANPQRA
                     LQLFQFAATNFGDANAQYNLARMHLDGAVGGKDGIEALRWLFLAADKGHVQAQALLGQ
                     LLFAGRDGVRPQRARGLMWLTLAREAAIDSNKDKWIIDLYETALASANEADRHEALAY
                     LENHLKRRN"
     gene            complement(1220..2950)
                     /gene="ilvD"
                     /locus_tag="VNY06_00025"
     CDS             complement(1220..2950)
                     /gene="ilvD"
                     /locus_tag="VNY06_00025"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012453817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HWX13264.1"
                     /translation="MDHRVFDKASLPSRYVTEGTSRAPHRSYLYAMGLTRKQIHQPLI
                     AVASCWNEAAPCNIALMRQAQAVKKGVAAAGGTPREFCTITVTDGIAMGHEGMKSSLV
                     SREVIADSVELTMRGHCYDALVGLAGCDKSLPGMMMAMLRLNVPSIFIYGGSILPGTF
                     KGQAVTVQDLFEAVGKYSVGEMAAEDLETLEMVACPSSGACGAQFTANTMATVSEAIG
                     LALPYSAGAPAPYEIRDRFCVTAGEMVMDLLEKRIRPRDIVTRKALENAATVVAASGG
                     STNAALHLPAIANECGISFDLFDVAEIFRKTPYIADLKPGGKYVAKDMFEAGGVPLLM
                     KTLFDHGFLHGDCLTVTGRTIAENLAKVAWNPDQDVVWPADKPMSLTGGVVGLKGSLA
                     PEGAIVKIAGMPDRALSFRGPALCFDNEEDCFEAVTNRRYREGCVFVIRYEGPRGGPG
                     MREMLATTAALYGQGMGSKVALVTDGRFSGATRGLCIGHVGPEAAVGGPIALLRDGDI
                     IEIDAIRGTINHQLGDAEIEKRKKDWHPRKTNFGSGALWKYAQIVGPAFSGAVTHGGA
                     LSETNVYADV"
     gene            3873..5180
                     /locus_tag="VNY06_00030"
     CDS             3873..5180
                     /locus_tag="VNY06_00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020176539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HWX13265.1"
                     /translation="MTDVVVQDRPANRAQTAIHEVVDGRRRGFRTILPMAGPAVVVSI
                     AYMDPGNFATNIQAGAKYGYGLLWVVLVANLIAMLFQALSAKLGIVTGRNLAEMCRDQ
                     LPRPLVFAMWIVSEIAAMATDLAEFLGGAIGLSLLFGLPLIWGMTVTAIVTYGILTFE
                     RRGFRPIELIIGALVGIICLCYLIELFIVPVDWKAAAFHTVVPQLEDAAAVTIAVGII
                     GATVMPHAVYLHSGLTQARMTVHNDADRRKLLRYSNREVIVALAVAGAVNMAMVMMAS
                     GAFHEGNSDVAEIESAYQTLRPLLGTGASTVFLISLLASGVSSSAVGTMAGQMIMQGF
                     VGFRIPVVVRRLVTMIPAFIVVGYGVNATNALVISQVILSIALPIPMIALLLFTQRAD
                     IMGPFVNGRATRLVAMLGTAVVLLLNTALLLQTFGVPIPFLGE"
BASE COUNT         1128 a         1528 c         1469 g         1132 t
ORIGIN      
        1 tgggcaaggt gatcgcttca attcatgaga aacgggacga attccggaaa atccttcggc
       61 ctgccccaag caccgccgga gagatccgcg caattaaaat gtcaaaccgg atgaaagaaa
      121 tatgacagca gcaaataaaa ccggaggagt gagcgttaca gcgtgcccag cggaagtggg
      181 aaccggccgc ctggacatgc aacaaacaaa gatttagaaa gacaacagcg catcgcgtgt
      241 ttttaggcgg aaaagccgtg acccggatag ccgccggatc actcgccagt ttgcccgttt
      301 ccgcttttta gtcaagcttg cgccgttggc gatcggacgc aaaggccgag ctcaccctga
      361 gccgccccgt cgacctcaat tgcggcgctt gagatggttc tcgagataag cgagcgcttc
      421 gtgccgatcc gcctcattgg ccgaggcaag cgctgtttcg tagagatcga taatccattt
      481 gtctttgttg gagtcgatcg cagcctcccg tgccaaggtc agccacatca ggccccgggc
      541 acgctgcggc cgaacaccgt cgcggccagc aaaaagcaac tgaccgagca aagcctgggc
      601 ttggacatgc cccttatcgg cggcgagaaa aagccaccgc agggcttcga taccgtcctt
      661 tcctccgacc gccccatcga gatgcatacg cgccagatta tattgggcgt tcgcatcgcc
      721 aaaattcgtc gcggcaaact gaaacaactg gagggcgcgc tggggattag ccacgacttc
      781 actgttctct atcccattca agctgtagat gcccaaggca acaaaagcgc tcgacacgat
      841 ggcggcactg cgcggatctg ggttgtcctc atcgtaatta ctgacgattt gagagaaata
      901 ctcataggcc tttagatcct cgcgcggaac accatcgcca ctggcataga ttttggcgag
      961 tttccattgg gccaaaagct ccccaccctc ggcggcatat tttaaggctt caaaggctct
     1021 ctcagtatcg ccgagacgaa agccctcaag cgctgtccgc aacgctgcct ggggggtcgc
     1081 ataaatttga agcggcgctt tttcgacgat ttggagcggc gctttttcga cgcgggcccc
     1141 atcggccgcc tcgaatgcgg cagcccgcgc cgctggcgcg gagagtccaa gccccagaac
     1201 gacagtgcct gcaatacggc taaacgtcag cataaacatt cgtttcactc agagcgccgc
     1261 catgcgtaac ggctccgcta aaggctggac ccacgatctg ggcatacttc cataacgcac
     1321 cagacccgaa attggtttta cgtgggtgcc aatctttttt tcttttttcg atttccgcat
     1381 cccctaattg gtggttgatc gttccacgaa tggcgtcgat ctcgataatg tcgccgtcgc
     1441 gcagaagcgc aatcggcccg ccgacggcgg cctccggccc gacatggcca atgcaaagac
     1501 cgcgggttgc gccggaaaat cttccatcgg tcacaagcgc gaccttcgat cccataccct
     1561 ggccgtaaag agccgccgtt gtcgcaagca tttcgcgcat gccgggtcca ccgcggggcc
     1621 cctcatagcg gatcacaaaa acacaacctt cccgatatct gcgattggtg accgcttcga
     1681 agcaatcctc ttcattgtcg aagcagagcg ccggaccccg aaagctcaaa gcccggtcgg
     1741 gcatgcccgc gatcttcacg atcgcccctt cgggcgcgag gcttcccttt aggccgacaa
     1801 cgcccccggt cagactcatg ggcttatccg ccggccaaac gacatcttga tcggggttcc
     1861 atgcgacctt ggccaaattc tccgcgatcg tccggcccgt aaccgtgaga cagtcgccat
     1921 gcagaaaacc gtggtcgaac agcgttttca tgagaagagg cacgccgcca gcctcaaaca
     1981 tgtccttggc gacatatttg ccccccggct tcagatcggc gatatagggt gtcttgcgga
     2041 aaatctctgc gacgtcgaaa agatcgaaag aaattccgca ttcattggcg atcgccggaa
     2101 ggtgcagtgc ggcattggtc gagccgccag atgccgccac aacggttgct gcgttttcaa
     2161 gcgctttgcg ggtcacaata tcgcgcggcc ggattctttt ctccaggaga tccatcacca
     2221 tttcgcctgc ggtgacgcag aaccggtcgc gaatctcata gggcgctggc gccccagccg
     2281 aataaggcag ggcaaggccg atcgcctccg aaacggtcgc catggtgttg gcggtaaact
     2341 gcgctccaca ggcacctgat gaagggcagg caaccatttc gagcgtttcc aaatcctcgg
     2401 ccgccatttc tccgaccgaa tatttgccaa ccgcttcaaa aagatcctgc accgtcaccg
     2461 cttggccctt aaaagtgccg ggcaagatcg aaccgccgta aatgaaaatg gatggcacgt
     2521 taagacgcag catcgccatc atcatgccgg gcagggattt gtcacatccg gcaaggccga
     2581 cgagcgcatc atagcaatgg ccgcgcatgg tgagctcgac cgaatcggcg atcacttccc
     2641 gcgacacgag agaagacttc attccttcat ggcccattgc gatgccatcg gtcacggtga
     2701 tcgtacaaaa ctcgcgcggc gtgccgcccg ccgcggcaac accctttttc accgcttgag
     2761 cctgccgcat taaagcgata ttgcagggcg cggcttcgtt ccagcaggac gcgacagcta
     2821 tgaggggctg gtgaatctgc tttcgggtaa gtcccatggc gtaaaggtag gagcggtgcg
     2881 gcgcccgcga tgtgccttcc gtcacatacc ggcttggaag gcttgccttg tcgaacaccc
     2941 gatgatccat aggagctcgt tccgagccta tcggccgatt gtctgattga ggaagcggca
     3001 ccgctgtgga tcgtgccgcg cggggaagcg agcgaaactg tctcgcaatg cccgccgaac
     3061 actacccagg tagttgaggc ttgacgttaa aacttacact agcaagttgt tgaagaataa
     3121 aaaagctgct tcttcttgca gcgccaagtc aaagttaact tgccgcgcgc tgtgacatca
     3181 aaagcacaat ttgtgtcact tggcgagcca atttctgtaa cgtgcatcgt tcctttgaaa
     3241 acaaattgta acgaaacatt tgtcttgtga tggcgaccgg cgcgttcagg ctcgcaaaac
     3301 gcgaatcacc cttcggccga tgagcttggg agattgaacg acgcgttcaa gctgtgcgcc
     3361 caacagcgct cggtctgagt actcggcgac gcgcagcgct ttcggctcct taatgcggac
     3421 aaggttagaa ggtcgtcggc aagccttaag cgtgtcgagc caaaagtcgg aaccggtttt
     3481 gcgtccggac acgcgacgaa acaagattta gggcaatatg acgatgcgaa ggaaagccat
     3541 cttgctctag gcagccgcgg gcacggaaag ccaatccgcg ccgctctctc ctcgggaaag
     3601 aagtcgcgtt cgagtggaga tcgcagctct catcccgaca acgatgggcg gggccgtgtg
     3661 cgtggtacgg ccattgatgt tccaggaccg atgcgcatgg attaatgcac attgattatt
     3721 ttgtagccta ggctatatgt tatagcgtca acatagaaac aagaggctgc caccgcgcct
     3781 ccctgcgtcg aggccggctt ttggccagac gagggaattt cttgggtgtt gccgagactg
     3841 tatttgttga ggttccgagc aaggaagaac agatgacgga tgtggtcgta caagatcgcc
     3901 cagcgaatcg ggcacagacc gccattcacg aagtggtcga cggtcgaagg cgcggatttc
     3961 gaaccatcct gccgatggct ggcccagccg tggtcgtttc gatcgcctac atggaccccg
     4021 gaaattttgc gactaatatc caggccggcg cgaaatatgg ttatgggttg ctttgggtcg
     4081 ttcttgttgc aaatcttatc gcgatgttgt ttcaggccct ttcggcgaaa cttggcatcg
     4141 tgaccggccg caacctcgcc gaaatgtgtc gtgaccaatt gccgcggccc ctcgtcttcg
     4201 ccatgtggat cgtcagcgag atcgcggcga tggcaaccga tctcgcggag tttcttggcg
     4261 gcgcgatcgg actctccctg ctgttcggtc tcccgctcat ctggggaatg accgtcaccg
     4321 cgatcgtgac ctatggaatt ctgacatttg agaggcgagg cttcagaccg atcgagctga
     4381 tcatcggcgc cttggttggg atcatctgtc tctgttatct catcgaattg ttcattgttc
     4441 cggtagactg gaaggccgcg gctttccaca ctgtcgttcc gcagctcgaa gatgcagcag
     4501 cagttacgat cgcagtgggc atcattggcg ccaccgtcat gccgcatgcc gtctaccttc
     4561 attccggcct cacccaggcc cggatgacgg tgcacaatga tgcagatcgg cgtaagctcc
     4621 ttcgctactc caaccgggaa gtcatcgtcg cactggcggt cgccggagcc gtgaatatgg
     4681 cgatggtgat gatggcgtct ggtgcttttc acgaaggcaa cagtgacgtc gccgagatag
     4741 agtcagccta ccaaacgttg cgtcctctgc ttggaacggg agcgtccact gtcttcttga
     4801 tttccctcct tgcttccggc gtgtcgagct ccgctgtcgg aaccatggcc ggccagatga
     4861 tcatgcaagg cttcgtcgga ttcagaattc cggttgtggt ccggcggctt gttacgatga
     4921 taccggcatt catcgtcgtc ggctatgggg tcaatgcgac caacgcgctc gtcataagtc
     4981 aggtcattct cagcatcgcc ctgccgatac ccatgatcgc gcttttgcta ttcacgcaac
     5041 gagccgacat catgggcccg tttgtcaatg gccgtgcaac tcgtttggtg gcgatgctcg
     5101 gcaccgcagt ggtgttgctc cttaacactg cgcttctgtt gcagacgttc ggtgtgccta
     5161 taccattctt aggggaataa gccgcaccat ctcggcgcca atacggggag cgatccatgg
     5221 ctttgccggg caggtttatc gctttgccag cgatttg
//
LOCUS       DATMPU010000003         3074 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_1863698,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000003 DATMPU010000000
VERSION     DATMPU010000003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 3074)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3074)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3074
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1863698"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..160
                     /locus_tag="VNY06_00035"
     CDS             <1..160
                     /locus_tag="VNY06_00035"
                     /inference="COORDINATES: protein motif:HMM:NF022060.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate oxidase C-terminal
                     domain-containing protein"
                     /protein_id="HWX13266.1"
                     /translation="LGEVPRPPYWQGYRIVPLYFEFWAEGAFRLHDRLVFQRQTELHS
                     WRQERLYP"
     gene            439..930
                     /locus_tag="VNY06_00040"
     CDS             439..930
                     /locus_tag="VNY06_00040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX13267.1"
                     /translation="MVTEEPVKSESKADPGEANQENFDYDDDGYEAFVALMWTQPGID
                     LKKIHAERVWRSGEVFPAILVQLVESNGRLYINLYEGKTRKTYAADNRHSWRRNGAVI
                     DSSAFQAIKLGVDPEAALSPDSYPASFEAALGPAHNRVAAVGRAFVRTSVGSPGCPYW
                     RNN"
     gene            1012..1587
                     /locus_tag="VNY06_00045"
     CDS             1012..1587
                     /locus_tag="VNY06_00045"
                     /inference="COORDINATES: protein motif:HMM:TIGR02301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02301 family protein"
                     /protein_id="HWX13268.1"
                     /translation="MPENTETTGKCGNPSGVLGVIAASAICFLLSAIPAGAQFEFLFG
                     RPEPTPPAASAKPAEKPAKAKPRKPKPKDAKTVPATNAPVTAVEEPPPPFDPELLKLA
                     EILGALAYLDDLCATKPPGDWREKMKALLEAEAKTSARKERLAGSYNRGYRDYERTYH
                     FCTQNAQAVIGRFLAEGGKIAHGVVSRYGGS"
     gene            1664..2671
                     /locus_tag="VNY06_00050"
     CDS             1664..2671
                     /locus_tag="VNY06_00050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020175337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HWX13269.1"
                     /translation="MAAGLKNLITDIPGICVGHAHDAVIASGVTALIFEAPAVASIAV
                     HGGAPGLRDTALLEPGMTVERVDGLVLSGGSVYGLDSMGGVISWLSEKGRGYAVGNVA
                     VPIVPGAVLFDLLNGGDKSFGREPTYWRLGYKAAAAAADNFELGSVGAGYGATTANLK
                     GGIGSASVTTSTGFRVGALVAVNALGQATISDGPHFWAAPYECQLEFGGLGWPAPWPK
                     DALGVHIKSAAAENTTIAIVVTDAALTKSESRRLAIMAHDGMARALRPSHACLDGDTI
                     FAAAIGIAKSLPNVSDRIEIGLSAADCLSRAIARAVYEADALPFPGALPSWKDRHRRS
                     S"
     gene            complement(2876..>3074)
                     /locus_tag="VNY06_00055"
     CDS             complement(2876..>3074)
                     /locus_tag="VNY06_00055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX13270.1"
                     /translation="LPSSHAGRVVLQKPFRLEQLLEALRTEFGLAADGYLGFEEDLSQ
                     CRDRHAKARHSSGVPTSSPGL"
BASE COUNT          668 a          814 c          909 g          683 t
ORIGIN      
        1 actaggagag gttccccggc ctccctactg gcagggctac cggatcgtac cgctttattt
       61 tgaattctgg gcggaagggg cgtttcgtct gcacgatcgt cttgtgttcc agcggcaaac
      121 ggaactgcat agttggcgcc aagagcgtct gtatccatga atatcgcggc cacgactgcg
      181 gcagcgtttt tgaccgcgaa taccgaggcg gggaggggca tatcctgcgg ccatattttc
      241 gatttatcgg catcgttttc cagccgggcc ggcgagcaag ccaagtgccc tttgctggca
      301 gcttttcttg cctggcggtc tagttaggat gggctagtac atcgacgtta gatagaaatc
      361 gatatgggat acaataaatc tcgtcccacg atcctcgtta atttgtggtc atcgatttga
      421 tctcggttcg gcggtcgctt ggttaccgag gaacccgtaa agagcgaaag caaggccgat
      481 cccggggaag caaaccaaga aaatttcgat tatgacgacg acggttacga agctttcgtg
      541 gccttgatgt ggacacaacc cggtatagat ttgaagaaaa tccatgccga acgagtttgg
      601 agatcgggtg aagtgtttcc cgcaatactg gttcaactgg tggagagcaa tggtcggctg
      661 tatatcaatc tttacgaagg aaagacgcgc aaaacatatg cggctgataa ccgacattct
      721 tggcggcgaa atggtgcagt aattgattct tctgcctttc aggcgatcaa gttgggtgtt
      781 gatccagagg cggctcttag ccctgattcc tatccggctt cctttgaagc agctctcggg
      841 cctgctcata atcgcgtggc tgcagtaggg cgcgcctttg tacgaacgtc tgttgggagt
      901 ccgggatgcc cttactggcg aaacaattga gttagataat ttcagaaaga acaaaagcac
      961 cgcgaaaaaa gtgacgcgca cgtcgacgca agcgatcggt gaaaagaaac catgccggaa
     1021 aacactgaga ccacgggaaa gtgcggcaat ccaagcggcg tgctgggcgt gattgccgca
     1081 tcggcaatct gtttccttct gtcggcgatt ccagccggag cccaatttga gtttttgttc
     1141 ggccgaccgg agccaacgcc tccagctgcc tcggccaaac cggctgagaa gcccgcaaaa
     1201 gccaaacccc gaaagcctaa gcccaaagac gctaagactg tgcccgccac caatgcgccg
     1261 gtgacggcgg tggaagaacc tccgccacct tttgatcccg agcttctgaa gctagccgaa
     1321 atccttggtg cattagccta cctcgacgac ctctgcgcca ccaagccccc tggcgattgg
     1381 cgtgaaaaaa tgaaggcgct attggaagcc gaggccaaaa caagtgcgcg caaggagcga
     1441 cttgcgggca gctataatcg cggctatcgc gactatgaaa ggacctatca tttctgcacg
     1501 caaaatgcgc aagctgtgat cggccgcttt cttgccgaag gcggaaagat cgcgcatggc
     1561 gtggtgagcc gttacggcgg gtcatgatgt tgggccagta gagctggcta cttaacactt
     1621 gctgaaagat ttcccatcga cgagtcgtcg cggaagtttg tgaatggcgg cagggttgaa
     1681 aaatctcatt accgatatcc ctggtatttg cgtcggccat gcgcatgatg cggtaatcgc
     1741 gtccggggtt accgcgctga tcttcgaggc ccctgctgtt gcctcgattg ccgttcacgg
     1801 cggtgcgccc ggtctgcgtg atactgcatt gctcgaacct ggaatgacgg tcgagcgggt
     1861 tgatggcttg gttctttcag gtggctcagt gtacggcctc gattcaatgg gcggcgtcat
     1921 aagttggcta agtgagaaag gtcggggcta tgcggtgggc aacgtcgcgg tgcccatcgt
     1981 gccgggtgca gttcttttcg accttctcaa cggaggggac aagagttttg ggcgcgaacc
     2041 cacctattgg cgtttgggat acaaagcagc ggctgcggcg gccgacaatt ttgagcttgg
     2101 cagtgtcggt gccggctatg gcgcgacaac cgctaatctc aagggcggca tcggctcggc
     2161 tagcgtaact acatcgactg ggtttcgagt cggcgctctc gttgcagtga atgcgctcgg
     2221 ccaggcgacg atcagcgatg ggccgcattt ctgggctgct ccttacgaat gccagctgga
     2281 atttggcggc ctcggctggc ctgcgccttg gcctaaggac gcgcttggcg ttcacatcaa
     2341 aagtgccgca gcggaaaata ccaccatagc aattgttgtt accgatgccg cgctgacgaa
     2401 atcagaatca agaaggcttg ccattatggc tcatgatggt atggcccgag cgttgcgccc
     2461 atcgcatgca tgcttagatg gtgatacaat attcgccgct gcgatcggaa ttgcgaaaag
     2521 cctcccaaat gtcagcgaca ggattgagat cggcttaagt gctgctgatt gtctttcccg
     2581 tgctatagcc cgcgccgtct acgaggctga cgcattgcct tttcctggtg ctcttccaag
     2641 ctggaaagac cgccaccggc ggagttcctg aatccgcccc gggtctattg gtgcgttagg
     2701 agtcgacgat ccgccgagcc gcaggcaagc gcaatcaccg agtattgggg ctcttcctcg
     2761 gcgcgctgcc gtgaaaataa cggcgctgcc ggtcctgagc ggtggattct cgcttgaaag
     2821 gcgttttctc aggcttcagg ctatgggcga tcccaacagc cgttgattcg gaggctcaga
     2881 gccccgggct ggaagtgggg acaccggatg agtggcgtgc ctttgcgtgg cgatcacgac
     2941 actgtgataa atcctcttca aacccaagat agccatcggc cgccaaccca aactccgttc
     3001 gcagggcctc taaaagctgc tccagccgaa acggcttttg cagcacaaca cggccagcat
     3061 ggctggaagg caaa
//
LOCUS       DATMPU010000004         5239 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_2173392,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000004 DATMPU010000000
VERSION     DATMPU010000004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 5239)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5239)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5239
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2173392"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            457..615
                     /locus_tag="VNY06_00060"
                     /pseudo
     CDS             457..615
                     /locus_tag="VNY06_00060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015916821.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
     gene            1030..2138
                     /locus_tag="VNY06_00065"
                     /pseudo
     CDS             1030..2138
                     /locus_tag="VNY06_00065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011925466.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
     gene            2437..4605
                     /locus_tag="VNY06_00070"
     CDS             2437..4605
                     /locus_tag="VNY06_00070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012591320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amylo-alpha-1,6-glucosidase"
                     /protein_id="HWX13271.1"
                     /translation="MESIVDTAIEFPQHYIEIEGSLVQRSLRSLKHGDAFAVFDNYGN
                     IGVIGTGPEGLYFNDTRFLSWYDLRFEGKRPLLLGSVVHDDNAALSINLANPDVHADG
                     LISLPRDTIAIERTKFLRQAVCYERIGFRNYADSTIHFRIEIGFGADFRDLFEVRGTS
                     RKRRGTCTSLVSEGRAEFHYEGLDNLKRKTELQFWPPPSRLETNRATFEFELAPNARQ
                     SLFVSVLCDDGNKPKSDLFGRAFRAGRRAIRRKATGIATIESSNELFNEVCQRATADL
                     YMLMTRTRHGLYPYAGIPWYSTVFGRDGIITAMLLLWLDPSVAKGVLGLLAETQAKTF
                     DHFSEAQPGKILHETRNGEMARLGEVPFRQYYGTVDATPLFVMLAGMYFDRTGDIGTI
                     AAIWPNIKAALQWINTFGDADEDGFVEYARQSGSSLVNQGWKDSYDSIFHANGALASG
                     PIALCEVQGYVFAAKTQAARLAHCIGEYDLEIKLRGEAQILRKNFEAAFWCEDLGTYA
                     LALDGDKNPCRVRTSNAGHALFTGIAAQERAARVAETLLGPDSFSGWGIRTVASGEAR
                     FNPISYHNGSVWPHDTAMIALGLARYGFVWHATKVFSAMFEAAAYQDLRRLPELFCGF
                     IRKPHRGPTAYPVACAPQAWASAAPFAFLGACLGMELRHEVNSVSFRDPVMPSFLDYV
                     ILNQLSLGNSRIDLKLQRHGSDVTLNLLRRRGDAKVMLVK"
     gene            complement(5010..>5239)
                     /locus_tag="VNY06_00075"
     CDS             complement(5010..>5239)
                     /locus_tag="VNY06_00075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX13272.1"
                     /translation="MLHAVGEGAVMARAKTGSAYMAGRASAAFLAWYWLLPSWSGWLA
                     PYKETRFKKIRDAIEGRESRLFLETAEMITL"
BASE COUNT         1081 a         1542 c         1527 g         1089 t
ORIGIN      
        1 cagcggcgat cttggtgcaa caaagcaata aattgaatca tgcgcgacac tccgccgcgt
       61 tgcaaaatat cttctgaggc accaggcgcg cagcatcgaa gcggttatag atcctcgtcg
      121 gcggccgccg ggcaatcgca accagcgaca gccgcttttc aacacctgca cgatgcagcc
      181 atgatcgcac gagcgatgtt gtcctggata gcaaacacgt cctgcatttc gcggtcgaac
      241 cgctccgacc agtggctgaa accgtcggcg acctacgagg tcggagcggc agcggtcaac
      301 atcgggcgac ctctgtggac cgcagtcccc cgatcatctc cagaatgcgc caccaatgga
      361 acgccgagac atgctccgag ttaagcatgg gcccctcatt tgaggaggcg gtgctgacga
      421 tttttccaac ccaagcatcg tttgggaaaa atatccatga acaaggaccg agtcgccgga
      481 tcggcgaaga agatcaaggg ctcaatcaag gaggtggtcg gcgaggcggc ggggacgctc
      541 gaagtcgaag gaaaagccga caagggtgaa ggcaaggttc agagcgccgt cggtggcctc
      601 aaagacacgc tcaaatcaac agcttcgacg aggttgaggg caaccagcca gtgcccggca
      661 ggagcgcagg ggtatctcag agtgcaagcc ccgcttcgca aacaagactt gccaagccga
      721 gccgctcgcg aaggcgcagc gacgacagct tagtttgaac cgcagatacc agcacacgca
      781 gaggatgatg atcacccggt cgaaatggcg gcctttgaaa agatcatcaa tgctcaacat
      841 gctggtcggc ttcaccgcgg ggctattttg ccgccaatta tgcactgctt ccgctctttg
      901 cagcagaact ggctacaaag tctttgcgcc agaaccgggc acaaaagtag ctttggtttt
      961 atgaaccgac gcgcgcgatg aaaacttcgg gaacctccgc gcgctaagca cattgcgaaa
     1021 ggaaaccaaa tgcgcctcgc gcttgtcgca cctctcacgg aagccgtgcc cccgaagctc
     1081 tatggcggaa cggagcgcgt tgtgtcctgg ttggcggaag agcttgttag gcaagggcac
     1141 cgggttgctt tatttgccag cgcggagtcc gagacggcgg cggagcttgt cgtctgcgcg
     1201 ccacaaagcc taagacttgc cgggattcgc gaccacctgg gcagcaccct cgccatgctg
     1261 cgcgaggtga ggcgccgcgc cgatgacttc gacatcatcc attttcatgt ggacctgttg
     1321 ccgcaagccc tgttccacga tcttgcccac aaatgtctcg tgacactgca cggacgcctc
     1381 gatctacccg atgccttgcc tctctataag gcctatccgg agatgccgtt ggtttcgatt
     1441 tcaaacgcgc agcgccgtcc aatgccaccg aatgccaatt ggctcgccac gatcccgcac
     1501 ggcctcgtgt cgcacgtctg cccgttcaac ccggatggcg gcggctattt ggctttcctc
     1561 ggacgtatct cgcccgaaaa gcgcccggac cgcgcgatag aaatcgccaa gcgagccggg
     1621 atccccttaa aactcgcggc aaaggtggac gccgttgata ttgcatattt caagaccgag
     1681 atcgaaccgg ttcttgacca tcccctcatc gagttcatag gcgaaatcgg cgaaacccaa
     1741 aaaagcgaat ttctcggcaa cgcgcgcgcg cttttgtttc cgatcgactg gccggagcct
     1801 ttcgggctcg tcatgatcga agcaatgtcg gcgggaacgc cggtgatcgc ttggcccaac
     1861 gggtccgtgc cggaagtcat tgcggacggc gtcagcggcc gtatcgtcga ttccatcgag
     1921 gctgcggcgg gagctgcgcg acaggccgct tacatggacc gccgcaaggt tcgcgccgag
     1981 ttcgaacggc gtttcaccgc cgagggatgg cgggcgccta tatcgcggcc tatcgtttac
     2041 tattggcgcg cgctccggcg actacgacga taaatacgaa gcttttggag cctaccatgg
     2101 cgccacctgc cgcgcccgag cttctgccat cggcttgaga acaagtggct ggggaatgag
     2161 acgccccggc cttgaacccg gcgggttgac gagatagacc acggaacgaa ggctaaaact
     2221 ggcgcgctga acattgtcac gaggccgccg atgggcattg tcgctgttgc ttcctctcta
     2281 gaccgtgatt gctttaagtt gaatcggtca gggtctccat cactttgttt tcttgcatga
     2341 tctatccaaa aaggttgcag ctttttagga tcatgctcta aaacccggtt cacgcggaga
     2401 acggacatcg caggcaacac ggaggcggtg cttcggatgg aaagcatcgt ggacacggcg
     2461 atagagtttc cccagcacta tatcgagatc gagggatccc tcgttcagcg atcgctgcgc
     2521 agcctgaaac atggcgatgc tttcgcggtc ttcgacaatt acggcaatat cggtgtcatt
     2581 ggcacgggac ccgaaggact ctatttcaac gacacccgct ttttgtcctg gtacgatctc
     2641 cgttttgagg gcaaacggcc gttgttattg ggctcggtcg ttcatgacga caatgccgct
     2701 ctctcgatta atcttgccaa ccccgacgtg catgccgacg gcctcatttc gctgccgcgc
     2761 gacacgattg caatcgagcg caccaaattc cttcggcagg cggtctgcta tgagaggatc
     2821 ggttttcgca attacgccga tagcaccata cattttcgca tcgagattgg gtttggcgcc
     2881 gattttcgcg atctgttcga ggttcgcgga acctcacgca aacggcgcgg cacctgcacg
     2941 tcattggtca gcgagggccg cgccgagttt cattacgagg ggctcgacaa tttaaagcgc
     3001 aagacggaat tgcagttctg gccgccgccc tctcgtcttg agacgaacag ggcgacgttc
     3061 gaattcgaac tcgcgcccaa cgcgcgccaa tcgctcttcg tgtcagttct ttgcgatgac
     3121 gggaataagc cgaagagcga tctttttggc cgtgctttcc gggcggggcg gcgcgccatc
     3181 cggcgcaagg cgaccggcat cgcgacgatt gaaagttcga acgaactctt caatgaagtc
     3241 tgccagcggg ccaccgccga cctctacatg ctgatgacgc ggacccgcca tgggctttat
     3301 ccctacgcgg gaatcccatg gtacagtacg gtgttcggtc gtgacgggat catcacggcc
     3361 atgctgcttt tgtggctcga cccgtccgtc gccaaaggcg ttcttggcct tctcgcagag
     3421 acgcaggcaa aaaccttcga tcatttttcc gaggcgcagc ctggcaagat tctgcatgag
     3481 acacggaacg gggaaatggc gcgtcttggt gaagtgccat tcagacagta ttacgggacc
     3541 gtcgatgcaa cgccgctctt tgtcatgctc gctggcatgt atttcgaccg gaccggggac
     3601 atcggaacga tcgcggcgat ctggccgaat atcaaggcgg ctttacaatg gatcaacacg
     3661 tttggcgatg ccgatgaaga cggctttgtc gaatatgcgc ggcagagcgg atctagcctg
     3721 gtcaatcaag gctggaagga ttcttacgat tcgatcttcc atgcgaacgg cgcattggcg
     3781 agcgggccta ttgcgctttg cgaagtgcaa ggctacgtgt ttgccgcaaa aacgcaggcc
     3841 gcgagactcg cgcattgcat aggcgagtat gatttggaga tcaagctgcg cggcgaggcg
     3901 caaattctgc gaaagaattt cgaggcggct ttttggtgcg aggacctcgg cacctatgcc
     3961 ttggcgctcg acggcgacaa gaatccatgc cgggtccgga catcgaatgc gggccatgcg
     4021 ctctttaccg ggattgcggc gcaggagcgg gcagcgcgcg tcgccgagac attgcttggc
     4081 ccggattcct tcagcggttg gggaattaga acggtcgcca gcggcgaggc gcggttcaat
     4141 ccgatttctt atcacaatgg ctcggtctgg ccgcacgaca ccgcaatgat cgcgcttggc
     4201 cttgcccgtt atggcttcgt atggcacgcg accaaagtct tctccgcgat gttcgaagct
     4261 gcagcctatc aggatttgcg ccgcttgccg gaattgttct gcggcttcat ccgcaagccg
     4321 caccgcgggc cgaccgccta tcccgttgct tgcgcgccgc aagcctgggc gtcggccgcg
     4381 ccctttgcct ttcttggagc ctgtcttggc atggagctgc gtcatgaagt caattccgtt
     4441 tcctttcgcg atcctgtcat gccctctttt ttggattatg tgatcttgaa ccaattgagt
     4501 cttggcaatt cgcgcatcga ccttaaactc cagcggcatg gcagcgatgt caccctcaat
     4561 ttgttgcgcc ggcggggcga tgcgaaggtc atgcttgtga aatgagccgc gcccgaggtc
     4621 ttgtacgccc cggacaatgc gggccggcgg acgacttcat gatcgctgtc gtcatggagg
     4681 cgttccagga tcttgcggac aagcccatcg tcatcgtcag catcgatcag atattcgacg
     4741 ccgtggagaa cgacctgctt tcgcaggaat acctcggcgc atcgggaatc cgctccatct
     4801 ccgaatcgga tgagcgcgta ccccgggatg cctgtcgagt cctcacgcgt actgaaaaat
     4861 tctctggctg ctgcagtgga tcacgtgggt tccaagcgtt cccggatcgc gccgagataa
     4921 agcatgagcg ccgcgaccga ttttccacct gcgcggtgat gcgatcgccg ggcttgccca
     4981 agttcggcga gcgcctctgc gtagcgtccc taaagcgtga tcatctccgc ggtttcgaga
     5041 aaaagccggc tctcgcgccc ttcgatagca tcgcggattt ttttgaaccg agtctcttta
     5101 taaggcgcca accaaccgga ccacgacggc aacaaccaat accaggccaa gaacgccgcc
     5161 gaggcccgcc ccgccatata tgcgctgccg gtcttggcgc gtgccataac cgccccttcg
     5221 ccgacggcgt gaagcatgc
//
LOCUS       DATMPU010000005         4617 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_3723830,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000005 DATMPU010000000
VERSION     DATMPU010000005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 4617)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4617)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4617
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3723830"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..233)
                     /locus_tag="VNY06_00080"
     CDS             complement(<1..233)
                     /locus_tag="VNY06_00080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013167858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin family protein"
                     /protein_id="HWX13273.1"
                     /translation="MTIQIGDSIPKTSFVIMTPDGPEQRSSDQIFKGKKIVLFGVAGA
                     FTPVCDKNHLPGSLMETETLRARSGFRLISQRAG"
     gene            complement(364..1242)
                     /locus_tag="VNY06_00085"
     CDS             complement(364..1242)
                     /locus_tag="VNY06_00085"
                     /inference="COORDINATES: protein motif:HMM:NF022853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD
                     domain-containing protein"
                     /protein_id="HWX13274.1"
                     /translation="MRVGIIIACASLLQLQFPGAGRCDPYTSGWAFGPKSALRLIAAG
                     GGPPDSAYRAGIELQLEPGAFTYWRMPGSAGIPPEFSFEGSANAAEITVSYPAPVRFV
                     DGGIEVFGYRDQVIFPLRIKPQDAASPVRLVLTLSYGVCAALCLPGKGEASLTFFPGD
                     SGTASVSLEARLVAAADALVPMRLSPQERDAKIAITRDETAPLPSWRFSLRADTAQDL
                     FAEAPQGWYFDTKATAQPNEFLIVEADRPTEGGSKRPPVILTVKSERQSYEFAVDLDA
                     ASDQRKDAQLAPKSVP"
     gene            1381..2001
                     /locus_tag="VNY06_00090"
     CDS             1381..2001
                     /locus_tag="VNY06_00090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012383725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HWX13275.1"
                     /translation="MGSIKLPTPSPQSPSGFLDGQLLIAMPGIVDDRFARSVIYVCAH
                     SEEGAMGIIVNRPAERVDFPQLLVQLKVIGPDEAIRLPAEASSLLVLNGGPVDTSRGF
                     VLHSNDFSIDNSTLPIGGGVSLTATIDILRAIASGSGPGQAVLALGYAGWAAGQLEAE
                     IQQNGWLNCPADPSLIFDSALDSKYERAMRRAGIDIGRLSSGAGHA"
     gene            complement(2173..>4617)
                     /locus_tag="VNY06_00095"
     CDS             complement(2173..>4617)
                     /locus_tag="VNY06_00095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012332207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HWX13276.1"
                     /translation="TLDPGTTVTYVAELRTPNLPQLQLWDPNAYKDRMASLALYRGIV
                     IGVAGLLALFLSIIFVVRGAVIFPAAAALAWAVLAYACIDFGFWAKIFGTGGESDRVW
                     RAATETTLSATLVVFLFAYLKLNRWHVRASHVAAAWLMFLAALALLAVYDAPVAAGIA
                     RISLATIASAGFVLVLYLAMHGYDRAIMLIPAWFLLLVWVIGAAFTVTGKLTNDLVSP
                     ALIGGLVLIVMLIGFTIMQNAFAGSGLAAGVLSDSERKALALTGSGDIIFDWDVAGDR
                     IYVTPDIEVQLGLARGALEGPATAWLNIVHPRERDRFRACLDTILEQRCGRINQEFRL
                     RGDGADYFWFRFKARPVSGADGEVVRIVGTLADVTEAKVAEERLLHDAVHDNLTGLPN
                     RELFFDRVDQALMLAQTDNCIRPAIISIDIDRFKQVNEAAGLSAGDSILLTLSRRLAR
                     TLKPQDTLARLSGDQFVVLIVSEFDTAHISAFANLVRRVVSTPVTFNEREIPLTASIG
                     LALYDPQLRTTREDMVKDAEIAMRHGKRMGGNRIEVFRPSMRTLRSDRFGLDADLRRA
                     LDRGEIKVLFQPIVRLEDRTVAGFEALLRWDHPRLGRLGPADFIPLAEETGTIVDLGI
                     FALERTARELAAWQHALDVDPPIFASVNVSSRQLLRHDLLHDVRAVLTRSNVSPGTLK
                     LELTEGLVMENPEYAAQMLTRIRELGAGLSLDDFGTGYSSLAYLQRFPFDTIKIDQSF
                     VRQNGKGSRPVMLRSILTLAHDLGMEVVAEGVETESDAIELDQLGCEYAQGYAFGHPL
                     SAIEARKLVGAATVAA"
BASE COUNT         1036 a         1291 c         1373 g          917 t
ORIGIN      
        1 cccgcgcgct gtgagatcaa gcggaagccc gagcgcgcgc gcaaagtctc cgtttccatc
       61 agcgaccctg gcaaatggtt tttgtcgcaa actggcgtga aggcaccagc cacgccaaaa
      121 aggactattt ttttgccttt aaaaatttga tccgaactgc gctgctctgg gccgtccggg
      181 gtcatgataa cgaaagatgt cttggggatg gaatcgccaa tctggattgt catctgtgct
      241 cctttatcaa acgccaaagc tccactcagg ttggcatcat gccatgacgg ttcccagcgg
      301 gcagcgaaaa tgctgcatca taactggaag ggcgattctt gttatttatt cgttttgatc
      361 agatcacgga acagacttcg gtgccaattg agcgtctttc ctttgatcgg aagcagcatc
      421 tagatcgacg gcgaactcgt aactttgccg ttcacttttc accgtgagga tcaccggagg
      481 gcgcttggag ccgccttctg ttggccggtc tgcttcaacg attaagaatt cgtttggctg
      541 ggcggtggct ttcgtgtcga aataccatcc ttgaggcgcc tcggcgaaga ggtcttgggc
      601 ggtatcggca cgcagcgaaa accgccagct tggcaatggc gccgtttcgt cgcgggtgat
      661 ggcaattttg gcgtcgcgtt cctgcggcga cagacgcata gggaccaaag cgtccgccgc
      721 ggcgacaaga cgcgcttcca ggcttacgga agcggttccg gaatcgccag ggaaaaaggt
      781 cagcgacgcc tctcctttgc cgggtagaca cagggcggcg caaacaccat aggaaagggt
      841 cagcaccaat ctcacgggac tggccgcgtc ctgaggcttg atacgaagcg ggaaaatcac
      901 ttgatcgcga tatccaaaaa cctcgatgcc gccatctacg aatcgaaccg gcgccggata
      961 ggatacggtg atttcggcgg cattcgcaga gccttcaaac gaaaattccg gcggtatacc
     1021 ggcactgccc ggcatacgcc aataggtgaa ggctccgggc tcaagttgga gttcgattcc
     1081 ggcgcggtag gcgctatcgg gcgggccgcc tccagcggca atcagccgca acgcggattt
     1141 tggtccaaac gcccacccgc tcgtataagg atcgcaacgt cctgctcctg gaaactggag
     1201 ctgcaaaagg ctcgcgcaag cgataatgat accaactctc attgagagct tgaaagctgg
     1261 aaaggctttt gcattagatt gagccgaact ctcggggacg tggcggcaac ttaatgcgta
     1321 cgagtgaaac ccgcaacaat ggttggttca atttggcttg acttgtgaga ggcgcttgac
     1381 atgggatcga taaaattgcc aacgccttcg ccgcaaagcc cgagcggttt tcttgatggg
     1441 caattgctca ttgcaatgcc cgggattgtc gatgatcgct ttgcgcgctc ggtgatttac
     1501 gtgtgtgccc attcagagga gggcgccatg gggatcatcg tcaaccggcc ggcggaaaga
     1561 gtagatttcc ctcagcttct tgttcaactc aaggttattg gtcccgacga ggcgataagg
     1621 ctgccagcgg aagcaagttc cttgctcgtt ctgaatggtg gcccggtcga taccagccgg
     1681 ggatttgtgc tccactcgaa cgattttagc atcgacaatt ccactttgcc gatcgggggt
     1741 ggcgtgtcct tgaccgcaac aatagatatt ttgcgtgcga tcgccagcgg ctccggtccg
     1801 ggtcaggcag tgcttgcctt gggctatgcc ggttgggccg ccggccagct cgaagccgag
     1861 attcagcaaa atggctggct caattgtccg gcggatcctt cccttatctt cgattcggcg
     1921 cttgattcga aatatgaaag ggcgatgcgc agagcgggaa tcgacatcgg ccggctatcc
     1981 tcgggcgccg gtcacgcctg atcccaatga ccacacacac attctaggcc accgagagcc
     2041 cggaccagtt cattcccgcc aaatccctaa gtgccgctcg cgctgaactc agagcaagat
     2101 gacttttgct caatcatcat ctcgctctaa atccatctag acgcaagccc agtgttcaat
     2161 catcatctcg ctttaggcgg cgactgtggc ggcgcccacc aatttgcgcg cctcgatcgc
     2221 gcttagggga tggccgaaag catagccctg cgcatattcg cacccgagct gatcgagttc
     2281 gatcgcgtcc gattctgttt cgacgccttc cgccacaacc tccatgccaa ggtcgtgggc
     2341 gagtgtgagg atagaacgga gcataacagg ccgtgagcct ttcccattct ggcgtacaaa
     2401 ggactgatcg attttgatcg tatcaaacgg aaagcgctgc agataggcca aggacgaata
     2461 gccggtgcca aaatcatcca gcgaaaggcc ggcgcccaat tcgcggattc ttgtgagcat
     2521 ttgggccgca tattccgggt tctccatgac gagcccttcg gtcaattcaa gttttaaggt
     2581 gcccggacta acgtttgacc gggttagcac cgctcgtacg tcgtggagaa ggtcgtggcg
     2641 gagcaattgg cgcgaggaga cgttgacact tgcaaaaatt ggcggatcga cgtcgagtgc
     2701 gtgctgccat gcggcgagtt cgcgggcggt gcgctccaaa gcgaaaatcc cgaggtcgac
     2761 aattgtgcca gtctcttccg caagagggat gaagtccgcg gggccaagtc ggccgagacg
     2821 cggatgatcc caccgtagca aggcctcgaa gccggcgact gtgcggtctt caagccggac
     2881 gattggctgg aatagaacct tgatttcgcc gcggtcaagc gcgcggcgca ggtccgcatc
     2941 gagaccgaag cggtccgaac gcagggtgcg catcgagggc ctgaagactt cgatgcggtt
     3001 gccgcccatg cgcttgccat gccgcatcgc aatttccgca tctttgacca tgtcttcgcg
     3061 cgtcgtccgc agctgcgggt cataaagcgc aagcccgatc gacgcggtga gcggaatttc
     3121 ccgctcattg aacgtcacag gggttgacac aacgcggcgg acgagattcg cgaaggcgct
     3181 aatatgagcc gtgtcgaact ccgaaacgat caaaacaaca aactgatcac ccgaaaggcg
     3241 ggcgagcgta tcctgcggct tcagagtgcg ggccaatcgc cgcgagagag taagcagaat
     3301 ggaatccccg gcagaaaggc ccgccgcttc attgacctgc ttgaaccggt cgatgtcgat
     3361 gctgataatt gcggggcgga tgcaattgtc ggtttgcgcg agcatcagtg cctggtcgac
     3421 gcgatcgaag aaaagttccc ggttcggcaa gccggtgaga ttgtcatgca cggcgtcatg
     3481 gagaagcctt tcctcggcga cctttgcctc ggtgacgtcg gcgagagtac caacaatacg
     3541 aacgacttct ccgtcggctc cgctgaccgg ccgggctttg aagcgaaacc agaaatagtc
     3601 tgcgccgtca ccgcgcagcc gaaattcttg attgatccgg ccgcaccgct gctcgaggat
     3661 ggtatcaagg caggcacgga aacggtctct ttcgcgggga tgcacaatgt taagccaggc
     3721 ggtcgccggc ccttccagcg ccccacgggc caggcccaat tgaacctcga tgtcgggagt
     3781 cacatagata cgatcgcctg caacatccca gtcgaagata atatcgccgc tgcccgtcag
     3841 tgcaagagcc tttcgttccg agtcggagag aacgccagcc gcaagcccgc tccccgcgaa
     3901 ggcgttctgc atgatcgtaa aaccgattag catgacgatc agcacgagcc cgccaatgag
     3961 ggccggggag acaaggtcat tggtgagttt gccagtgacc gtgaaggccg cgccgatcac
     4021 ccaaacgaga agcaggaacc aggcgggaat aagcataatt gcccggtcgt aaccatgcat
     4081 cgccaagtaa agcacgagaa cgaatccagc gctggcaatc gtcgccagtg agatccgggc
     4141 aatgcctgcc gcaacgggcg cgtcataaac ggcgagcaag gcaagcgcgg cgagaaacat
     4201 cagccatgcc gctgcaacat gggaagcccg cacatgccat cggttgagct tgaggtaggc
     4261 gaacagaaaa actacaagtg ttgcggagag ggtggtctca gtggcggcgc gccaaacgcg
     4321 gtccgactcg ccgccggtgc cgaaaatctt ggcccagaaa ccgaaatcaa tgcaggcata
     4381 ggcaaggacc gcccaggcga gcgctgcggc ggcggggaag atcacggcgc ctcgaacgac
     4441 aaagatgatc gatagaaaaa gcgctagcag cccggcaacc ccaatgacga ttcccctata
     4501 gagggcaagg ctcgccattc tgtccttgta ggcattcggg tcccagagtt gtagctgcgg
     4561 aaggttgggc gtccgaagct ctgcaacata ggtaacggtc gttccggggt cgagggt
//
LOCUS       DATMPU010000006         2141 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_3104559,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000006 DATMPU010000000
VERSION     DATMPU010000006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 2141)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2141)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2141
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3104559"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..501
                     /locus_tag="VNY06_00100"
     CDS             <1..501
                     /locus_tag="VNY06_00100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX13277.1"
                     /translation="TGGGLKKAAYFFLENSGAAEEPFVLHNVDVISTIDLHRMAQFHA
                     ENRALATLAVQERPTSRYLLFDEQLQLCGRKSGRDGQAELVRNSSHPKPLAFSGIHVI
                     SPRLFSLMTEEGVFSIIAPYLRLAAQGEKIIAFRSDEYQWRDLGRLDDVMQADQDARQ
                     LSGRPK"
     gene            complement(554..1198)
                     /locus_tag="VNY06_00105"
     CDS             complement(554..1198)
                     /locus_tag="VNY06_00105"
                     /inference="COORDINATES: protein motif:HMM:NF025033.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HWX13278.1"
                     /translation="MNPAAFVAEAIADFGTVGAIAPSSRYLTQAMLRPLPLEDARVVV
                     ELGPGTGVMTRAMLDVMPSNATLLTFEINPRFARHLRSTIKDPRLVVIHARAEALLEE
                     LSRRGYRHVDAMLSSLAMGLMSDRERHALLSEISISLRKKGTFTQYQYLHGLQFRDRQ
                     LSKFDMFGLLVRYFRSVQRTITWRNLPPAFVFTCRGARLSTPHDSIEAPAFRKK"
     gene            1416..>2141
                     /locus_tag="VNY06_00110"
     CDS             1416..>2141
                     /locus_tag="VNY06_00110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010684187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HWX13279.1"
                     /translation="MRHEVQTNEARDRRADLSRRRILKQAGWATIAAACRPWLGLAAD
                     QVSPAMTRLSGYMAEARTRALPEEVIEKTKHHVLDTIASMVSGSELPPGKAAIQFARA
                     YGGEKIATVVASNVLCGPIEAALANGVLAQADETDDSHAPSLSHPGCAVVPAALAAGE
                     RFPTDGAQFLRAVALGYDVGTRVSMTLGGQKFMDETHRDTHSLSEGFGAAAAAGCAAN
                     LNAQQMRWLLDYAAQQSSGISFWM"
BASE COUNT          441 a          647 c          608 g          445 t
ORIGIN      
        1 accggcggcg gcctgaaaaa agccgcttac ttcttcctgg agaattcggg ggccgccgag
       61 gagccgttcg ttctgcacaa cgtcgacgtc atcagcacga tcgatctgca ccgcatggcg
      121 caatttcacg ccgaaaatcg cgctctcgca acccttgcgg tgcaagagcg ccccaccagc
      181 cgctatctgc tctttgacga gcagctacag ctttgcggcc ggaaatcggg cagggacggc
      241 caggccgaac tcgttcgcaa ctcatcgcac ccgaagccgc tggctttctc cggcattcac
      301 gtcatttctc cgcgtctttt ctcgttgatg acggaagaag gcgtgttttc catcatcgcg
      361 ccttacctgc gccttgccgc acagggcgaa aaaatcattg cgtttcgttc cgatgagtat
      421 caatggcgtg atctgggcag gctggatgac gtgatgcaag ccgatcagga cgcgagacag
      481 ctttccggca ggccaaagta gatgatctga ttcacgcgcc tatttacaaa cacgtacccg
      541 gcgacaagcc gatttacttt ttcctgaacg cgggtgcttc gattgagtca tgcggggtgc
      601 tgagcctggc gcctcggcag gtgaagacga aggcgggcgg caggttgcgc caggttatcg
      661 tccgttgcac cgagcgaaaa tagcgcacca gaagcccgaa catatcgaac ttgctcagtt
      721 gccggtccct gaattgcaaa ccgtgcagat attgatactg cgtaaaagta cctttcttcc
      781 tgaggctaat actgatttcg ctcaatagtg cgtgtctttc gcgatctgac atcaagccca
      841 tggccagtga cgaaagcatc gcatctacgt gccggtaccc gcgccggctc agctcttcga
      901 gcagcgcttc cgcccgcgcg tggatgacca cgaggcgagg gtccttgata gtcgatctca
      961 ggtgtcgagc aaaccgcgga ttgatctcaa acgtcagcaa agtcgcgtta gaaggcatga
     1021 cgtccagcat ggcgcgcgtc atgacgccgg tgcccggtcc caactcaacc accacccgag
     1081 catcttcgag tggcagcggc cgcagcatgg cctgcgtcag ataccgcgaa ctcggcgcga
     1141 tcgcgcctac tgtgccgaag tcggctatcg cttccgcgac aaatgctgcc ggattcattt
     1201 tgtcatcccc gccttttgac cagcttaccc cacgatgctg gtgccgtcac tgctttaaac
     1261 tttgttgatt gctccgacgc gtatctacaa tcatggcatt acataatcgg ctgaagctga
     1321 agccagcctt aatccgggtt ccttggattg cattcgcgcc gatttttcgt ggtattaata
     1381 aacctgacga atggataccg gaaaggcgga ggtatatgcg acatgaagtg caaacgaacg
     1441 aggcacgcga tcgccgcgcg gatctaagcc gccgtcgcat tttgaagcaa gcaggctggg
     1501 cgacgatcgc cgctgcatgc aggccctggc tgggattggc ggccgaccag gtcagcccag
     1561 caatgaccag actcagcggt tacatggccg aggcgcgaac tcgtgcgttg cctgaggaag
     1621 ttatcgagaa aacgaagcac cacgtgctgg acaccatcgc ctcgatggtt tcaggctccg
     1681 aacttcctcc cggcaaggcc gcgattcaat tcgcccgcgc atatggcggc gagaaaattg
     1741 ccacagtagt tgcttcgaac gtgctctgcg gccccatcga ggccgcgctc gccaatggcg
     1801 ttctggcgca agcggacgaa accgacgatt cgcacgcccc atcgctttcc catccgggct
     1861 gcgccgttgt gcccgctgca ttagcagcgg gagaacgctt ccctacagac ggggcgcaat
     1921 tcttgcgcgc cgttgctctc ggatatgacg ttgggacgcg cgtctccatg actctgggcg
     1981 ggcaaaagtt catggatgag acacaccgcg acacgcatag cctttcggaa ggcttcggcg
     2041 cggccgccgc cgcaggctgc gcggcgaatc tcaatgcgca gcagatgcgt tggctcctcg
     2101 attatgccgc gcagcaatcc tccggcatct cgttctggat g
//
LOCUS       DATMPU010000007         3148 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_3725240,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000007 DATMPU010000000
VERSION     DATMPU010000007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 3148)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3148)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3148
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3725240"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..443)
                     /locus_tag="VNY06_00115"
     CDS             complement(<1..443)
                     /locus_tag="VNY06_00115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate ABC transporter
                     substrate-binding protein"
                     /protein_id="HWX13280.1"
                     /translation="MRRFVFVTIIGSFALIAAAGVALDYFERPTILRVAVPRDSDDQA
                     ILAAATRSFAESREDIRLKLVTVGDLAQSAHALEDGHADLAVVRSDLAMPPRGQTVLI
                     MRRNAALLVAPAQSGLHEIEELRGHRIGVLQGPSAGKADNQLLLDT"
     gene            complement(511..1992)
                     /gene="cysG"
                     /locus_tag="VNY06_00120"
     CDS             complement(511..1992)
                     /gene="cysG"
                     /locus_tag="VNY06_00120"
                     /EC_number="1.3.1.76"
                     /EC_number="2.1.1.107"
                     /EC_number="4.99.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018964120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0043115 - precorrin-2 dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0051266 - sirohydrochlorin ferrochelatase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="siroheme synthase CysG"
                     /protein_id="HWX13281.1"
                     /translation="MHIVNVVSPLCEYDEGCSPRMQGLACLPVFFALAGKRVVLSGGS
                     NAVLWKAELCQAAGAELEIFGTAPCPGLVSLARCSPAVNLVTRRIAPKDFHGAAFVIA
                     ETKSSAEAEEFQAVARAAGVPVNIIDKPAFSDFQFGAIVDRSPLVIGISTNGGAPVLA
                     QALRGRLEALLPREIKYWAEKAKAWRETLLRFDIPPKMRRRFWELFGRDALAGRRAPQ
                     ERDLGALLAESEGSAPLAGSVALVGAGPGDPELLTLKALRALHSADVVLYDDLVAPPI
                     VEMSRREAEKIYVGKRGHKPSCRQDHILSLMVSLASQGKRVVRLKGGDPMIFGRATEE
                     ISALREQGISTEIIAGVTAALGAASRLQLSLTERKKARRLQFITAHAHDGKLPEDIDW
                     QALCDPHASTVVYMGVKTLEHLTARLLANGMDSATPALVVERATCPDERQIFGTIGTI
                     SERVAAARPTGPCVILIGEAFADALYLPRTHVKLETKPRVLAH"
     gene            2457..3140
                     /locus_tag="VNY06_00125"
     CDS             2457..3140
                     /locus_tag="VNY06_00125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018267033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HWX13282.1"
                     /translation="MTEDDYPPFHFALSDGHLSGFDVDLARAICEELNITCTIQARRF
                     DTLIDALNEDQGDAMIAAIRIDTRSRAKLDFSRPYYTTPARFVSTVTLEHVAPTPESL
                     RGKTVGVLAHSAHEAYLETYFPETIREAYETQELLRGALINGSIDTIFGDGISLSLWL
                     ESPAGQNCCRFRGGPFLDANLFGEGVGIAVKKGNSQVRQALDYALALLAQHGTYTTLY
                     LKYFPIGFY"
BASE COUNT          680 a          951 c          858 g          659 t
ORIGIN      
        1 gtgtccaaga gcagctgatt gtcagctttt cccgccgaag ggccctggag aacgccgatc
       61 ctatgcccgc gaagttcctc aatctcatgc agcccgctct gcgccggcgc cacaagcagc
      121 gcggcattgc ggcgcatgat caaaaccgtc tggccacgcg ggggcattgc cagatcgctg
      181 cgcacgacgg caagatcggc atgcccgtct tcgagcgcgt gcgccgattg cgccaggtcc
      241 ccgaccgtga ccaatttgag gcggatgtct tcgcggctct cggcgaaact ccgggtagcc
      301 gccgccagaa tcgcctgatc gtcgctgtcc cgaggaaccg caacgcgaag gatggttggg
      361 cgttcgaaat aatccagcgc cactccggca gcggcaatca gggcaaagct tccgataatc
      421 gttacgaaaa cgaaacggcg catctgaacc tctccgcctc atttgcaggc ttgaggacat
      481 taacctgagc tcgccgtcaa aagttgcgcc ctaatgagcc agcactcgcg gcttcgtttc
      541 gagcttgacg tgcgttcgcg gcaaatacaa ggcatccgcg aaagcctcgc caataagtat
      601 gacgcagggc ccggttggac gagcagcagc gaccctttcg gaaatggttc caattgtgcc
      661 aaaaatctgg cgctcatccg ggcaggtcgc tcgctcgacc acgagcgctg gcgtggcaga
      721 atccatgcca tttgcgagca agcgcgcggt caggtgctca agagttttca ctcccatgta
      781 aacgacggtc gatgcatgag gatcgcagag cgcctgccag tcgatgtcct cagggagttt
      841 tccatcatgt gcatgggctg tgatgaactg cagccgtctg gcctttttgc gctcggtaag
      901 ggacagttgc agccgggagg ccgcaccaag cgccgcggtg acgccggcta tgatttcggt
      961 tgaaatgcct tgctcccgca aggcagagat ttcctccgtg gcgcgcccaa aaatcattgg
     1021 gtccccgccc ttcagccgca ccacccgttt gccttgcgaa gccagcgaaa ccattagcga
     1081 caggatatga tcctggcggc atgacggctt gtgaccacgc ttgccaacgt agattttttc
     1141 ggcctcgcgc cggctcattt caactatggg cggagcgacg agatcatcgt agagaacaac
     1201 gtcggcagag tgcagtgcgc gcaaagcttt gagcgtcagg agttctggat cgcccggccc
     1261 cgccccgacc aaggctaccg atccagcgag cggcgcactc ccttcggact cagcgagaag
     1321 cgcgccgaga tcccgctcct gcggtgcgcg tcttcccgcc aaagcgtcgc ggccaaacag
     1381 ttcccaaaat ctgcggcgca tctttggagg gatatcgaac ctcagcaggg tttcccgcca
     1441 agctttagcc ttttcagccc aatacttaat ctcgcgggga agcagggctt caagccggcc
     1501 gcgcaaagcc tgcgcgagaa caggtgcgcc cccgttggtc gaaattccaa tgacgagcgg
     1561 cgagcggtcg acgatcgcgc cgaactgaaa atccgaaaag gcgggcttgt cgatgatatt
     1621 aaccggtacc cccgcggcgc gcgcgacagc ctgaaactcc tcggcttcag cggaactttt
     1681 cgtctccgca ataacaaaag cagcgccgtg aaagtctttt ggcgcgattc tccgtgtaac
     1741 gaggttaacc gccggagaac atcttgccag agaaaccaat cctggacagg gcgccgtacc
     1801 aaaaatttcg agttccgccc ctgccgcttg gcaaagttca gctttccaga gcacggcgtt
     1861 cgagccgcct gaaagaacca cccgtttgcc agctagagca aagaaaacag gcaggcaagc
     1921 aagcccctgc attcgcgggc tgcagccctc atcatattcg caaaggggtg aaacaacatt
     1981 aacgatatgc aaatttttgc ctatccttta tccaggtcat tgatcaaaaa acgtcctcct
     2041 gatgccctgg agcccatcag catgccgcgt cgcaacctcc tccgaggccc ttttttgcga
     2101 gacagcaaat accttatgcc cgtccgcggt tcatgccaaa taaatttata cttaaattga
     2161 aattacgtag ttggtccagc aacggccggg gaaatatcca atgtctgctt gctaggagct
     2221 tggcgtcgtg gcaaggccgt gctcctatgc cgtaggctcc aaaccttaag ggaatctccc
     2281 tgagagttat ccgtgtccgc ctcaaatggc gcctttgcct cgctggcctc gccgccctgt
     2341 tcgttgcggc tccacagccg acacggacag ccaatgctgt tttcgtgccg agcttttggg
     2401 agccgcaagc tcgtcctggt aagcctgacc taaacaattt gcgaggcctt cgcttcttga
     2461 ccgaggacga ttatccccca tttcattttg ctctgtctga cgggcatctt tctggatttg
     2521 atgtcgatct ggcacgggca atttgcgagg agctcaatat tacctgtaca atccaagcgc
     2581 gccgcttcga taccctaatc gacgcgctga acgaggacca aggcgatgcg atgattgccg
     2641 caatacgcat cgatacgcgg tcgcgtgcca aattggattt ttcaaggccc tattacacca
     2701 cgccggcgcg tttcgtctcg acagtgaccc tcgaacatgt agctccgaca cctgaaagcc
     2761 tgcgcggaaa gacggttggc gttctagcgc atagcgcgca cgaagcctat ctcgagacgt
     2821 atttcccgga aacgattcgc gaggcctatg agactcaaga gctcctgcgc ggtgcgctta
     2881 ttaatggcag catagacacg attttcggcg atgggatatc gctcagcctt tggctcgaga
     2941 gcccggccgg ccagaattgc tgccgctttc gaggcggtcc cttccttgat gcaaatctct
     3001 ttggagaagg cgtcgggatc gcggtgaaga aaggaaattc gcaggttcgg caggctctcg
     3061 attacgctct cgcactcctc gcccagcatg ggacctatac gactctttac cttaaatatt
     3121 tcccgatcgg attctactga cctttcaa
//
LOCUS       DATMPU010000008         2998 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_2175248,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000008 DATMPU010000000
VERSION     DATMPU010000008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 2998)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2998)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2998
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2175248"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            316..471
                     /locus_tag="VNY06_00130"
     CDS             316..471
                     /locus_tag="VNY06_00130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX13283.1"
                     /translation="MRIHFLTSAHNSLSQRLLVELTERGHKVTVAIRLRRIPYLGDAL
                     WPTSVQY"
     gene            529..927
                     /locus_tag="VNY06_00135"
     CDS             529..927
                     /locus_tag="VNY06_00135"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HWX13284.1"
                     /translation="MADKSRARRHMPFYLVTAALAVVAAAVAQADATSASRGETEPVE
                     VRIVSTEFKFVPATIRVSIGRAVALVLDNTGAETEHGIVVPGFRFRLQAEAGKVVQRT
                     IVFDKAGEFDLLCDLPGHSEAGMRGKLIVR"
     gene            949..2034
                     /gene="nirK"
                     /locus_tag="VNY06_00140"
     CDS             949..2034
                     /gene="nirK"
                     /locus_tag="VNY06_00140"
                     /EC_number="1.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012109888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050421 - nitrite reductase (NO-forming)
                     activity [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper-containing nitrite reductase"
                     /protein_id="HWX13285.1"
                     /translation="MSQYRNGKFVGEWFIAGIAAALGMLLCSPGALAEDVPNAPATNE
                     AALPPLPQNLARVPAPAVAPPVNRKKPELVHVELEAKPVTGLLTDGTGYQYWTFNGTV
                     PGPMIRVRQGDTVELTLKNSSESPVSHSIDSHGVLGPGGGGKVTQTPPGATSVFQFKA
                     MNPGIYIYHCATPMIPFHLAHGMYGLMVVEPPGGWPKVDREFYVMQGDIYLKGDPAES
                     GVHEAAIDKMEEEKPDFVVFNGSNGSLAKERALKAKVGETVRIFFGNAGPNLVSSFHV
                     IGEIFDKIHPEGAAETLSNVQSTLVPAGGATMVEFKTQVPGTYILVDHSLGRLQRGAM
                     GFLEVEGPPNPQVFQSIKAGSLASGGH"
     gene            2119..2235
                     /locus_tag="VNY06_00145"
                     /pseudo
     CDS             2119..2235
                     /locus_tag="VNY06_00145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611716.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            2289..2459
                     /locus_tag="VNY06_00150"
     CDS             2289..2459
                     /locus_tag="VNY06_00150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX13286.1"
                     /translation="MKNGSIAIMAVGYRLGHVFLERAADIEACVSRMAVSQSCSSAQA
                     FQSVKSTKPPRV"
     gene            complement(2486..2716)
                     /locus_tag="VNY06_00155"
                     /pseudo
     CDS             complement(2486..2716)
                     /locus_tag="VNY06_00155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002711409.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
BASE COUNT          667 a          818 c          906 g          607 t
ORIGIN      
        1 atcgcgatta aaacattctc cggcaaggta acggttttgg gggcattgga gagccgctca
       61 tccgtgacgc ggggttgctt ggcagaaaac gagggatatt tgtccttgtc gcgaattcga
      121 tctcggaaca caatagcgcc ataaacacac tcatccgcga ttaatcacgc ggcgcgtccc
      181 ctgatcaggg gacgcggagg agacggtttt gtgacaatcg aaccgggcaa tacgactctt
      241 ctttcgaggg aatcagcccg cagaaatacc ggttccggtt cgtttcaatt aacaaaaaat
      301 atggggagga aactcatgcg aattcacttt ttgacctcgg cccacaacag ccttagccag
      361 cgccttttgg tcgagctgac tgagcgcggc cataaggtta ccgtggcgat cagattgcga
      421 cgcatcccct atctagggga cgcgctctgg ccgacgagcg tgcaatatta ggccttggag
      481 aagcagcgtt ccaacgcttt tctcaaaaaa aatgcgtggg cgggaatcat ggcggacaag
      541 agtagggccc ggcgtcatat gccattctat ttggttactg ccgccctagc ggttgtggca
      601 gctgccgtcg cgcaagcgga tgcaacgtcg gcgtcgcgtg gcgagacaga acctgtcgaa
      661 gtcaggatcg tttccacgga attcaaattt gtgcctgcga cgatacgtgt ctcaattggg
      721 cgcgcagtgg cgctcgtcct cgacaatact ggggccgaaa cagagcatgg catcgttgtg
      781 ccggggttca gatttcgctt gcaagcagaa gcaggcaagg tcgttcaaag gacaattgtt
      841 ttcgataagg ccggcgagtt cgatttgctt tgcgacctgc cgggtcacag cgaggcaggc
      901 atgaggggca agctgattgt ccgctgattt tgaataaggg ggaaaccgat gtctcaatac
      961 cgtaacggca aattcgttgg tgaatggttc attgctggca ttgctgccgc tctgggcatg
     1021 cttctttgca gtcccggcgc cctagccgag gacgtgccga atgctccagc caccaatgaa
     1081 gctgctctgc cgccactgcc ccagaatctc gcgcgcgtac ccgcgcccgc cgttgctccg
     1141 ccggtcaacc ggaagaagcc ggagctggtt catgtcgaac tggaagccaa gccggtgacc
     1201 ggactcctca ccgatggaac gggctatcaa tattggacct ttaatggcac cgtgcctggt
     1261 ccgatgattc gcgtccgcca gggagacacc gtcgaactca ccttaaagaa ttccagtgaa
     1321 agcccggtca gccacagcat cgattcgcat ggcgtgctcg ggcctggagg cggaggcaag
     1381 gtaacccaga ccccgcccgg tgcgacaagc gtcttccagt tcaaggccat gaatccggga
     1441 atctacatct atcattgcgc gacgccgatg ataccgttcc atcttgcgca tggcatgtac
     1501 ggcctcatgg tcgttgagcc gcctggcggc tggccaaagg tcgaccgcga gttttatgtg
     1561 atgcaaggcg atatctatct caaaggagac ccagccgaaa gcggcgtgca tgaggccgcc
     1621 attgataaga tggaagaaga gaagcccgat ttcgttgtct tcaatggcag caacggatcg
     1681 ctcgccaagg agcgtgcgct aaaagccaag gtcggcgaaa cggttcgcat ctttttcggt
     1741 aatgctgggc cgaaccttgt gtcgagcttt catgtcatcg gtgagatttt tgacaagatc
     1801 catccggaag gagcagccga gactctgagc aatgtgcaat ccacacttgt cccagcaggc
     1861 ggggcgacga tggtagaatt caagacgcag gtacctggga cttatattct cgtcgaccat
     1921 agcctcggcc gcctacagag gggcgcaatg ggattcctcg aggtggaagg accgccaaat
     1981 ccgcaagtgt ttcagagcat caaggcggga agtttggcga gcggcggcca ctgacgcagc
     2041 gcgcggcgtg gctcggacag cgcagaaaga aggcggggca gcaaatgcgt caagaacagc
     2101 tcgacgggcg gaggctacaa agggaaactt ctgagccccg cgcgccgccg agcctgcatc
     2161 gaccatgtcc gatccgagct gaaactgtcc gagcgacggg tctgccgtgt gctcggacag
     2221 aagcgttcaa cgcaggatgt tttgttgaaa ttgattggct gtagaaccga agcaggggat
     2281 ggcgcaccat gaaaaacgga tcgatcgcca tcatggccgt tggctatagg ctcgggcacg
     2341 tctttctgga acgagcagcc gacattgaag cttgcgtctc acgcatggct gtcagccaat
     2401 cctgttcgtc ggctcaggcc tttcaaagcg tcaaaagcac taaaccgccg cgcgtttgac
     2461 tgaggcacaa ctattccgaa ggctccgaga gcgcctcgaa gatcagtgcc gcatcaaggt
     2521 gggtgagccc ggaaccgccg acatcctcac gcacatagat gccggccatg ccaagcgccg
     2581 ccgcctcgcg catcacatcg atagggaaat gtttcttctg atcccattcg atggccttcg
     2641 gcgcgagttt ttccgccgcg aacgtggaag ccgtattgcg gatgacgatc cacggacacg
     2701 aaatagcagc aagcacgttc ctagatcgca agattattca ggaaacacga ttccgcgcct
     2761 gatagagcga tgaccactcg atggaggcgt acgatgctgg attgtttatc aggcgccgcc
     2821 ggccaaaatg gccttctgga aggagaatga tatgaaacgc ttcggtgtat tgctggcctc
     2881 ggccgcgctg ggcgcggcgt tggccgcaag cggtccggcg ctggcattcg gcggcggcca
     2941 tggcggtggt ttcggcggcg gccacatgag cggcggcttc ggcggcggtt ttcacggc
//
LOCUS       DATMPU010000009         4563 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_4037043,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000009 DATMPU010000000
VERSION     DATMPU010000009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 4563)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4563)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4563
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4037043"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            169..1215
                     /locus_tag="VNY06_00160"
     CDS             169..1215
                     /locus_tag="VNY06_00160"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HWX13287.1"
                     /translation="MDKLLQLDVRADDPVNSHKSVSRITLRQILLSGLEDVVHFGKTF
                     TRYFVRDDGKIVAEFTDGSYASGDVLVGADGGSSRVRHQFLPHAQRIDTGVVGLAGKL
                     ILNADNRNGIAQEFLDGMALISAPGGLGMFIAAQIFADETAAKTGFGGNAIDYEPATG
                     PFLDNTKSYVMWALSGRRQTWLPNKPAQLLGGDASKHIAQTAAAGWHKDLRDLIARTD
                     PATVSCLPIRTSVPVGPWQTQRITLLGDAIHSMTPFRGIGANVALRDAANLCEALCAA
                     NGGEKDLLESIHSYEAQMLRYGFRAVRESLKAMEQTLDDRVIVRSLNRTTFKMLQALP
                     PPVKRWIFRRMGEE"
     gene            complement(1178..1963)
                     /locus_tag="VNY06_00165"
     CDS             complement(1178..1963)
                     /locus_tag="VNY06_00165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020180545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HWX13288.1"
                     /translation="MECYAEPLSLAASPARVDTWVFDLDNTLYPATSDLWPKIDQRIT
                     LYLGAIFGLDGMSSRALQKYYYEHYGTTLRGLMEEHDIRPEDFLDFTHDIDRSSLLPD
                     PMLADAITALPGRKLILTNGSREHALLTAAALGLEAMFEDIFDIADAGFLPKPAAETY
                     ERFFDRHEIEPSRAVMFDDLGRNLIIPHQKGMTTVLVVPKPGQKVHREAFEITLGGAA
                     SYIDFVTSDLARFLHGLVLDPKVSEDHCCGALILRPCGGKSSV"
     gene            complement(2045..2941)
                     /gene="argB"
                     /locus_tag="VNY06_00170"
     CDS             complement(2045..2941)
                     /gene="argB"
                     /locus_tag="VNY06_00170"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011995692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HWX13289.1"
                     /translation="MNDPADPLRQEDVQAQAQQQAAILMQALPHMLRYDDTIVVVKYG
                     GHAMGDDKVARAFARDMVLLEQSGVNPVVVHGGGPQIGTMLGKLGIKSAFADGMRITD
                     RATMEIVEMVLAGSINKQIVGFINAEGGRAIGLCGKDGNMVLARKVPQTGKDIDLGFV
                     GEPAKVDTTVLDQVLGRELIPVLAPVAQDENGETYNVNADTFAGAIAGALNAKRLLLL
                     TDVAGVFGRDGKMIKQLRGSEIHGLIADGTITGGMIPKVETCIYALEQGVEGVVILDG
                     KMPHAVLVELLTDHGAGTLITR"
     gene            complement(3164..>4563)
                     /locus_tag="VNY06_00175"
     CDS             complement(3164..>4563)
                     /locus_tag="VNY06_00175"
                     /inference="COORDINATES: protein motif:HMM:NF014871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HWX13290.1"
                     /translation="RIDEDQDRQGGTARYAAAVKAERKRARAQNRYLICAHAGDTLSP
                     SLISSFDQGAHMIDLFNEAGLDIFVPGNHEFDFGKDVYFDRMKDARFAILAANLKDSD
                     GAVPPYHKESLLIEANGIKLALIGAAYEDTPTASRPGNLQFLPAIPAVVANAKSARAA
                     GADFVAAIVHADKLKGAALMNMHAVDLSGHNHDLDIDFDGRTAFVESEQDANYLTVVD
                     IDVIRTPDNSNGSLAWWPEFRIIDTANIEPDPKALAKVRDYQARLEKQIDAEIATLGS
                     PLDSRTDCVRLRECAIGNFIADAVRKAAAAEISIINGGGIRGNRLYAAGTKLTKRDVL
                     DELPFGNKTVLTTVAGSAILSALENGLSRIGTASGRFPQISGLAVVVDPAAAPGARVK
                     TATVNGEMLDPSRDYRLATNDFMARGGDGYGMLAGKSQVTRDSGTRLVALDVIGYAET
                     LKFVDAKVEDRIVIR"
BASE COUNT          981 a         1332 c         1279 g          971 t
ORIGIN      
        1 agcctcggcg tctatgagcg cgaccacgcg ccgacgagcc gcctccaagg ctaccgtgca
       61 atcctaccgg cagcaaagcc ctgcatgctt gccttcccga acatctttac aaggcgttcg
      121 ttgacagctg cggcctttcc accggcaaga taacgtgcga ccgagggcat ggacaaattg
      181 ctccagctcg atgtccgcgc ggacgatccc gtgaacagcc acaagtccgt gagccgcatc
      241 acattgcgtc aaattctcct gtccgggcta gaggatgtgg tccatttcgg aaagaccttc
      301 acgcgctact tcgtccggga cgatggaaag atcgtcgccg aattcaccga cgggagctat
      361 gccagcggcg acgtcctggt tggcgcggac ggcggatcgt cgcgggtgcg ccatcagttt
      421 ttaccgcatg cccaaaggat cgacaccgga gtggtcggct tagccggcaa gctgatctta
      481 aacgccgata accgcaacgg catagctcag gaatttttgg atggcatggc gctcatttcc
      541 gcgccgggcg gccttgggat gttcatcgcg gcgcaaattt tcgctgatga gaccgccgca
      601 aagacgggtt ttgggggcaa tgctatcgat tatgagcccg ccacgggacc ttttctcgac
      661 aataccaaga gctatgtgat gtgggccttg agcggcagac ggcagacttg gctgccaaac
      721 aaaccggcgc agttgcttgg cggcgacgct tcgaaacaca tcgcgcagac ggcggccgca
      781 ggctggcaca aggatttgcg ggatctgatt gcaagaacag accccgcgac agtctcatgc
      841 ctgccgatcc gcacctcagt gcctgttggt ccttggcaaa cccagcggat cacgctcctc
      901 ggggatgcta tccatagcat gactccgttt cgaggaattg gcgcaaatgt cgcattgcgc
      961 gacgcagcta atctttgcga ggcgctttgt gccgcgaatg gcggtgaaaa ggatctgctc
     1021 gagtcaattc atagctatga agcccagatg ctccgttacg gatttcgagc cgtgcgcgaa
     1081 tcattaaagg cgatggaaca aactctggac gatcgggtca tcgtccgctc cctcaatcgc
     1141 accacgttca aaatgcttca ggccctgccg ccgccggtta aacgctggat tttccgccgc
     1201 atgggcgaag aatgagggcg ccgcagcaat gatcctcact cactttggga tcgaggacca
     1261 aaccatgcaa gaatctcgca agatcgctcg ttacgaaatc gatataggac gccgcgccgc
     1321 ccagcgttat ctcgaaggct tcgcgatgaa ctttttggcc gggttttggc acgaccagca
     1381 cggtcgtcat ccctttctga tgcggaatga tgagattgcg cccaagatcg tcgaacatga
     1441 ccgcgcgaga aggttcaatc tcatgccgat cgaaaaatcg ctcataggtc tctgctgccg
     1501 gctttggcag gaagccagcg tcggcaatgt cgaaaatatc ctcaaacatc gcctcaagac
     1561 caagcgctgc ggcagtcaac aacgcatgtt cgcgcgaacc attggtcaaa atgagcttgc
     1621 gccccggcag ggcagtgatc gcatcggcga gcattggatc gggaagaagc gaggaacgat
     1681 cgatgtcatg cgtgaaatcg agaaaatctt cgggtctgat gtcatgctct tccatcaggc
     1741 cgcgcaaggt ggtcccgtaa tgctcgtagt aatatttttg cagcgcgcgg gacgacattc
     1801 catcaagccc gaagatagcg ccaaggtaga gcgtgattct ctgatcgatc ttcggccata
     1861 gatcggaagt cgccgggtag agagtgttgt cgagatcaaa aacccaagtg tcgacgcgcg
     1921 caggagaggc ggcgagcgac aagggctcgg cataacactc cattgacgtc aaatcctgat
     1981 catgtctggc tgcggcgccg gacgggctac cggggaaatc tttcaaccca ctgtccctca
     2041 tcattcagcg tgtaatcagc gtcccggcgc cgtgatccgt caaaagttcg acgagcacgg
     2101 catgaggcat tttgccgtcg agaataacca ccccttcgac gccttgctcg agcgcataga
     2161 tacaggtctc gaccttcgga atcatacctc cggtgatcgt gccgtcggca atgaggccat
     2221 ggatttcact cccacgcaat tgcttaatca tttttccgtc gcggccgaaa acacctgcca
     2281 cgtcggtcag cagcaagagc cgcttcgcgt taagggcgcc cgcgatcgcg ccagcaaaag
     2341 tatcggcgtt gacattatag gtttcgccat tctcgtcttg cgcgaccggc gcaaggacag
     2401 gaatgagttc gcgcccgagc acttgatcga gcacggtcgt atctaccttg gcaggctctc
     2461 cgacgaaacc gaggtcgata tcctttcccg tttgcgggac cttgcgcgcg agcaccatat
     2521 ttccatcctt gccgcaaagc ccgattgccc gcccgccctc ggcattgatg aagccaacga
     2581 tttgcttgtt gatcgaaccg gcaagcacca tttcgacaat ttccatcgtg gcccggtcgg
     2641 taatccgcat cccgtccgca aatgccgatt taatgccgag cttgccaagc attgtcccga
     2701 tctgtggccc gccgccatga acaacaaccg ggttgacgcc agactgttcg agcaggacca
     2761 tgtcgcgcgc gaatgcccgc gcaaccttgt cgtcccccat ggcatggcca ccatatttga
     2821 cgacaacaat cgtgtcatcg tagcgcagca tatgcggcaa ggcctgcatc aagatggcgg
     2881 cctgctgttg cgcctgggct tgcacgtctt cttgacgcag aggatcggca ggatcgttca
     2941 tcagttattg tctcaaggta aagccgcggg acttctagcc gtttatcagg gcaagctcaa
     3001 cgccgggata ggtacgattg gcaggccaga gggaggtttt gggggcgcaa gaggaaatgt
     3061 tttgtcatgc gggagccgcc gcgcgccgat tgcgctccta atgcggcacg gtgtcggacg
     3121 acattgtaac gcccccaaag cggcgcggct gtgtccattc cggctatcga attacgatcc
     3181 ggtcttccac cttcgcatcg acgaacttca gcgtttcggc gtagccgatg acgtcgaggg
     3241 cgacgagcct cgtgcctgaa tccctggtca cttggctctt acccgcaagc atcccataac
     3301 cgtcgccgcc gcgcgccata aaatcattgg tcgcgagtcg gtagtcccgc gacggatcga
     3361 gcatttcgcc attgaccgtt gccgtcttga ctcgcgcgcc tggcgccgca gcaggatcga
     3421 caaccacggc aagcccggaa atttgtggaa aacgccccga agccgtgccg atccgggaca
     3481 aaccattttc gagcgccgac aagatggcac tgcccgcaac agtggtcagc acggttttgt
     3541 tgccaaatgg aagttcgtcg agtacgtcgc gcttcgtgag cttggtaccc gcagcgtaga
     3601 gtcggttacc tctgatgcca ccgccattga tgatcgaaat ttcggctgcc gccgctttgc
     3661 gcactgcatc ggcaataaaa ttgccgatcg cgcattcgcg caaacgaaca caatcggtgc
     3721 ggctgtcaag cggcgagcca agcgtcgcga tttcggcatc gatctgtttt tcgagccttg
     3781 cctgataatc acgcacctta gccagcgcct tggggtcggg ttcaatgttt gccgtatcga
     3841 taattcgaaa ttctggccac caggcaagcg acccattcga gttgtccggc gtccttatga
     3901 catcaatatc gacgacagtt agataattgg cgtcctgctc cgattcgaca aaggctgtcc
     3961 ggccgtcgaa atcgatatcc agatcgtgat tatgtccgga aagatcgacg gcgtgcatat
     4021 tcatgagtgc ggcgcccttt agcttgtctg catgcacgat cgctgcgacg aaatcggcac
     4081 cagccgcgcg cgcgcttttg gcattggcaa ccactgccgg tatcgcagga aggaattgta
     4141 agttgccggg gcgcgaggcg gttggcgtat cttcataagc ggcgccgatg agtgcaagtt
     4201 tgattccgtt cgcctcgatc agcaaacttt ccttatgata gggaggaacc gcgccatcgc
     4261 tgtctttgag atttgccgca aggattgcaa agcgcgcgtc cttcatccgg tcgaaataaa
     4321 catccttgcc aaagtcaaat tcgtgattcc cgggaacgaa aatatccaga ccggcctcat
     4381 taaagaggtc gatcatatgc gcaccttggt cgaaactcga tatcaggctc ggcgacagcg
     4441 tgtcgccggc atgagcgcaa ataagatagc gattttgggc gcgcgccctt ttgcgctctg
     4501 cttttaccgc cgccgcgtaa cgcgccgttc cgccttgccg atcctggtcc tcgtcgatgc
     4561 ggt
//
LOCUS       DATMPU010000010         2995 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_626958,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000010 DATMPU010000000
VERSION     DATMPU010000010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 2995)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2995)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2995
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_626958"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(74..841)
                     /locus_tag="VNY06_00180"
     CDS             complement(74..841)
                     /locus_tag="VNY06_00180"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020174846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HWX13291.1"
                     /translation="MLRPPRSSAPRATSTAYLDVSHAGETYRINLRRLASARRFTLRV
                     RAATQDVVLTMPARGSLNEAKFFAARHAAWIGAKLRRLPERIPFVPGEAVPLRGILHR
                     IAHCASRRGAVWIESNAANMGSTPLLWVGGEELFVARRVRDYLVKEARRDIEAAVRSH
                     ASRLKISARKVSLRDNVSRWGSCSSSGALSFSWRLIMAPHFVLDYLAAHETAHLLHMN
                     HSEAFWATVARLTPDTERAETWLKLHGSGLLRFGPDR"
     gene            complement(953..2242)
                     /gene="phaZ"
                     /locus_tag="VNY06_00185"
     CDS             complement(953..2242)
                     /gene="phaZ"
                     /locus_tag="VNY06_00185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012386230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyhydroxyalkanoate depolymerase"
                     /protein_id="HWX13292.1"
                     /translation="MPPFAYEMHELTHIALAPMRAMTDVARTWLESPINPVSYTAAGR
                     NLAASARVFERLTRRYDKPLFGLDTVSVGGTIVPIVERVIWQRPFCKLLHFEKRSDAA
                     PSELQQKLLLVAPMSGHHATLLRGTVEAFLPFYDVYITDWVDARSVPLGRGLFDLDDY
                     IDYLVAMCAVLGKEQDGRLLHTIGVCQPAVPLIAAIALMEAADSPHIPESMTLMGGPI
                     DTRRSPTKVNLLAQERGSEWFEQNCICLVPFGYRGAGREVYPGFLQLTGFMAMNIDRH
                     VTAHLEFFDHLVKGDGDSARKHREFYDEYRSVMDLPAEFYLQTVDTVFVKHALPKGQM
                     KHWGTPVDLRAIRHAGLLTVEGELDDISGLGQTAAAQDLCSSIPQARKAHYQQNKVGH
                     YGVFNGSRFRNEVVPRVLEFHRSLAPLSRHLKLVDRG"
BASE COUNT          685 a          865 c          839 g          606 t
ORIGIN      
        1 ctgcacccgt ttgacccccg ccaccgtggg gccgagctgg cttggcacgc accaattagc
       61 ggtcgggacc gaattagcgg tcgggaccga aacgaagcag cccggaaccg tgcaacttga
      121 gccaagtttc agcgcgctca gtgtcaggcg tcaggcgtgc gacggtggcc caaaaagctt
      181 cagaatgatt catgtgcagc aggtgcgctg tttcatgcgc cgcaagataa tcaaggacga
      241 aatgtggtgc catgattagc cgccaggaga agctgagcgc gccagaagag gaacatgatc
      301 cccaccggct tacgttatcc ctaagggaga ccttacgcgc ggagattttt agcctgctag
      361 catgagacct gacggctgcc tcaatatctc tcctggcctc cttgaccaaa tagtcccgca
      421 ctctgcgcgc cacgaaaagc tcttcgccgc caacccaaag caaaggcgtg ctgcccatat
      481 ttgccgcgtt ggactcaatc cagaccgcgc cacgccgtga ggcgcagtgc gcaatccgat
      541 gcaagattcc gcgcaaggga acagcttctc ccggaacaaa gggaattctt tcaggcaatc
      601 ggcgcagttt cgcgccgatc caggccgcat gccgggcggc gaagaacttc gcctcgttca
      661 ggctgccgcg cgcgggcatt gtcaagacaa cgtcttgtgt cgccgcccga acccgcaagg
      721 tgaaccggcg cgcggaggct aaccgtcgca aattgattcg ataggtctcg ccggcgtggg
      781 agacgtcgag gtaagctgtg gaagtagcgc gaggcgcgct gctgcgcgga ggccgtagca
      841 tagccgcaga atagctagtc gtccgcgcag tcgctagagc gtgcagatta gatcttcaca
      901 gctcctggtg gagggcacac tggcagtgtt ggttcatgag ttgccggcgc gctcagccac
      961 ggtcaaccag cttcagatgc cgggagagag gcgccagaga gcgatggaac tccaagaccc
     1021 tgggcacgac ctcattgcgg aaacgcgagc cgttgaacac gccgtaatga ccgaccttgt
     1081 tctgctggta gtgcgctttc cgtgcttgcg gaatgctgct gcagagatct tgcgccgccg
     1141 ctgtctggcc aagcccggag atgtcgtcga gttctccttc gacggtgagg aggcctgcgt
     1201 ggcggatggc ccgaagatca accggggtac cccagtgctt catttgaccc ttgggcagcg
     1261 cgtgtttgac aaaaaccgta tcgacggtct gcaggtagaa ttccgctggc aaatccatga
     1321 ccgagcggta ctcgtcgtag aactcgcggt gctttctcgc tgaatcgcca tcgcccttga
     1381 cgaggtggtc gaaaaactcc aaatgcgccg ttacgtggcg atcgatgttc atcgccatga
     1441 atccggtaag ctgcaaaaaa cccggataga cttcccgtcc ggctccgcga taaccaaatg
     1501 gcacgaggca aatacaattt tgctcgaacc attcgctgcc acgttcctgc gccaaaagat
     1561 tgaccttggt cgggctgcgc cgcgtatcga tcggaccgcc catcaatgtc attgactctg
     1621 gaatatgcgg gctgtctgcc gcttccatca aggcgatcgc cgcaatgagc ggaaccgcag
     1681 gctggcagac tccgatcgtg tgaagcagtc tgccgtcctg ctccttgcct agaaccgcgc
     1741 acatcgcgac cagatagtcg atatagtcat cgagatcaaa caggcctctg ccaagaggca
     1801 ccgaccgcgc atcgacccag tccgtgatat acacatcgta aaatgggagg aacgcctcca
     1861 cggtaccacg cagcaaagtt gcgtgatggc ccgacatcgg cgcaaccaac aaaagcttct
     1921 gctgcagctc gctcggcgca gcatcagacc tcttttcgaa atggagaagt ttgcaaaatg
     1981 gccgctgcca aatgacgcgc tccacaatcg gaactatcgt gccgccgaca ctgaccgtgt
     2041 caagaccaaa gaggggcttg tcgtagcgtc gcgtcaatct ctcaaatact ctcgccgaag
     2101 cggcgagatt ccggccggcc gcggtataag agaccggatt gatcgggctc tcaagccaag
     2161 tccgcgcaac atcggtcatg gctcgcattg gagccaaggc gatatgcgtt aattcatgca
     2221 tttcataggc aaatggcggc attttcttta tcctcgccca tcaatcaagc gtccttgccc
     2281 gtcaaacgaa catgccttgc ggttctcacg gtgctggcac gccaaagaac aatcgcggct
     2341 cgcgagcaga tgaagaactt gatgggcagc aacacccaag acgggattgt gttcccgccg
     2401 gcggatatgt ctaggcctgg gctccgacgg gaagatctca gtattgcacc atactgtcgt
     2461 taaatctaag taatggttga ttgcaaatca actatctgaa gctttccgat gtttatattt
     2521 ggttaagttt ggtggttgta aaaatatctt ttgacatttg aagtaattca tagtcgcacc
     2581 tcgctgacgg atcccgagtt cgtatgctgc gtaaacatgg tgatcacgcc tgcgatggcg
     2641 catccccctt cgttagaccg gaaataggct gcatggaact gcggaatcaa taaggcatca
     2701 aattggacac agaaaagcgc gtgcacccgg agcggaaagg cgatcagcta acttatgcaa
     2761 ttcgttgaat atgtggtgat ggctcaaaaa gtccgcaagc cccaaacgag ggatcgaatt
     2821 caccttcgca ctcgcacaca agggcgatag cggcctcctt accagaatgt tagcatgact
     2881 cctcgattgc cgtcagcgaa agggctggca tggccgacct tatcaacatc gaccttgggg
     2941 cccgctcgcg ccgcctgcgc gtcgatacgc tcgtcaaact ccgctggctt gccct
//
LOCUS       DATMPU010000011         4771 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_7122, whole
            genome shotgun sequence.
ACCESSION   DATMPU010000011 DATMPU010000000
VERSION     DATMPU010000011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 4771)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4771)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4771
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7122"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..234
                     /locus_tag="VNY06_00190"
     CDS             <1..234
                     /locus_tag="VNY06_00190"
                     /EC_number="1.17.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007692318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HWX13293.1"
                     /translation="EALNVAVMGCIVNGPGESKRADIGISLPGTGETPAAPVFIDGQK
                     AMTLRGDGIAAEFKEIVENYIERRFGGEKSAAE"
     gene            complement(432..2126)
                     /gene="ftsZ"
                     /locus_tag="VNY06_00195"
     CDS             complement(432..2126)
                     /gene="ftsZ"
                     /locus_tag="VNY06_00195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012592453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HWX13294.1"
                     /translation="MSINLKAPELRELKPHIMVAGVGGAGGNAVNNMIVSGLLGVEFV
                     VANTDAQALTSSKAERIIQMGLQVTEGLGAGSQPEVGRAAAEEAIEEIRDHLSGAHMV
                     FVTAGMGGGTGTGAAPVIARTARDMGILTVGVVTKPFQFEGARRMRIAEVGITELQKA
                     VDTLIIIPNQNLFRIANEKTTFADAFAMADQVLYSGVACITDLMVKEGLINLDFADVR
                     AIMREMGKAMMGTGEASGEKRAILAAEAAIANPLLDEVSMKGARGLLISITGGNDLTL
                     YEVDEAASRIRQEVDEEANIILGATFDQSLDGTVRVSVVATGIDQPASLAEPAIAEAR
                     IAEVTNRLRAQTAPRGLDGAQARLHASQPDAYAAAEYDSHYDERGFAAPSAGADHGVR
                     VQEIAPKHRPCLEQTPYAARPNDLYESGPFVPDAPTIRQQRMPQIEDLPLPAQNQMRA
                     HRGEPAPEHPEAKRRSSLLQKLASFGISRNDSEAPGVQSRERQAQPRPYAPGAYRQQQ
                     PNSLHAEYGKRTQHPPAPQQRAPQPAAEAQGRAAHQNRSYEEDQLEIPAFLRRQSG"
     gene            complement(2229..3545)
                     /gene="ftsA"
                     /locus_tag="VNY06_00200"
     CDS             complement(2229..3545)
                     /gene="ftsA"
                     /locus_tag="VNY06_00200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010685846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HWX13295.1"
                     /translation="MNSRPLPPRLPPLHAKKSAVLSVLDIGTSKIVCLVAKLNPSDAS
                     GALRGRTHSCRILGIGHQRSRGIKGGVVVDMDAAEAAIRFAVDAAERMAGVEVESVIV
                     NATGGRLASRLYEAKVAIAGRAVTQADVHRVLEACTASGSQQSRAVLHSLPNGFSLGE
                     TRHIQDPRGMVGDELSAEMNLLSCDAAAARNLLLSVERCHLSVDAMVATPYASALSAL
                     ADDEAELGTALIDMGAGTTSVSVFSGGHLTHVDSFAVGGHHVTMDVARGLGITVTDAE
                     RLKTLYGACLASVSDESETIAVVPAGEDYERPAHLPKAQLISIIKPRVEEILELLRDR
                     LKNAGLPPHAGRRLVLTGGACQLTGMQAAVKRIVSGQVRIGRPLGIKGLPESARSPAF
                     AASVGLLIYPQVSGNEHYRPSRRPLVREAKASGYIGRVGQWLWTSF"
     gene            complement(3542..4438)
                     /locus_tag="VNY06_00205"
     CDS             complement(3542..4438)
                     /locus_tag="VNY06_00205"
                     /inference="COORDINATES: protein motif:HMM:NF015739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HWX13296.1"
                     /translation="MVPAAFTLPAETRPPVRRPRRGLTQKILDILAKPGTAAFFSLAL
                     FTVVGVYGAIMGGHYAAFVSEEGEPADIIAKGLGFSIKAVTISGAHELKDQDILATAG
                     VGPRNSLLFLDAAKIREKLKLLPIVKEAAITKLYPDRLLIEVEERQPFALWQNDGETR
                     IVAEDGVVLGLIQDRRFIHLPFVAGAGANDKIGEYLALLESAANLRERIMAGVRVARR
                     RWTLKLTNGLEILLPERAPTAAMARLIELQRVYHILDKDVLLLDLRQPDRLIARLSAE
                     AAAARESAGAPPRTAKAKGGRT"
     gene            complement(4591..>4771)
                     /locus_tag="VNY06_00210"
     CDS             complement(4591..>4771)
                     /locus_tag="VNY06_00210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012592450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HWX13297.1"
                     /translation="RTDFRYDDLPGGTGELIVLEVNTQPGMTETSLVPELAGYAGITF
                     GELVRWMVEDASCDR"
BASE COUNT          952 a         1435 c         1434 g          950 t
ORIGIN      
        1 gaggcgctca atgtcgccgt tatgggctgt atcgtcaacg ggccaggcga atcgaaacgc
       61 gccgacatag gaatttcgtt gcctgggacc ggcgaaacac cggctgcgcc ggtattcatc
      121 gacgggcaga aggcgatgac tctgcgtggg gacggaatcg ccgccgaatt caaagagatt
      181 gttgagaatt acatcgagcg gcggtttggg ggcgaaaaaa gcgcggcgga atgagccttt
      241 cgtgatcgac ccccgtttaa cccggacagg tccgccagaa aatccagttc tgcggacccg
      301 ttttggccaa ttgcgagagc ggtccgcgcc tagagcaaga tggcttatct tcgaatcgtc
      361 atcttgctct aaatctttgt tctgtcgcgt gtccggacgc aatatcggtt cccacttttg
      421 ccggacacgc tctagcccga ctgccggcgc aaaaaggccg gtatttcgag ctgatcctct
      481 tcataggatc ggttttgatg ggcggcgcgg ccttgcgcct ccgccgcagg ctgcggtgcc
      541 cgctgctgcg gggcgggcgg gtgttgggtg cgtttgccat actccgcgtg aaggctgttc
      601 ggctgctgct ggcggtaagc cccgggtgca tacgggcgcg gctgcgcctg ccgttcgcgg
      661 gattgaacgc ccggggcctc ggagtcgttg cggctgatgc caaaagaggc gagcttttgg
      721 aggagagaag agcggcgctt ggcctcggga tgctcgggcg caggctcgcc gcgatgcgcg
      781 cgcatttggt tttgcgccgg cagcggcagg tcctcgatct gcggcattct ctgctgccgg
      841 atagtcggcg catcgggaac aaagggccct gactcataga gatcgtttgg ccttgcggca
      901 tacggtgtct gttcgagaca gggccggtgt tttggcgcaa tttcttgcac cctcaccccg
      961 tgatcggcgc ctgcggacgg cgcggcaaaa ccgcgctcgt cataatgcga atcgtactct
     1021 gccgccgcat aggcgtccgg ctgggaagcg tgaaggcgcg cctgggcgcc gtcaagcccg
     1081 cgcggcgccg tctgggcccg cagccggttt gtgacctcgg caatgcgcgc ctctgcaatc
     1141 gcaggttccg ccaggcttgc aggctggtca attccggttg caacaaccga aacgcggaca
     1201 gtgccatcga ggctttgatc gaatgtcgcg ccgagaatga tgttggcctc ctcgtcaact
     1261 tcctggcgaa tccggctcgc ggcttcatca acttcataaa gggtgagatc attgccgccg
     1321 gtgatcgaaa tgagcaggcc tcgggcgcct ttcatcgaga cttcgtcgag tagaggattg
     1381 gcaatcgcgg cctcggcggc aagaatggcg cgcttttctc ccgaagcctc gccggtgccc
     1441 atcatggcct tgcccatctc gcgcatgatc gcgcgcacat cggcaaaatc gagattgatc
     1501 aagccttcct tgaccatcaa atcggtgatg caggcaacac ccgaatagag aacctgatcg
     1561 gccatcgcga atgcatcggc aaaagtcgtt ttctcattgg cgatacggaa caaattttgg
     1621 ttcggaatga tgatcagcgt atcgacggct ttctgcaatt ccgtgatgcc tacctcggca
     1681 atgcgcatgc gtcgcgcgcc ctcgaactga aacggcttgg tgacaacgcc gacggtcaga
     1741 atgcccatgt ccctcgcggt gcgcgcaatg accggcgcgg caccggtgcc ggttccgccg
     1801 cccatgccgg ccgtgacaaa gaccatatgc gcaccggaaa ggtgatcgcg gatctcctca
     1861 atcgcttcct ccgccgccgc ccggccaact tcgggctgag atccggcacc tagtccttcg
     1921 gtcacttgaa ggcccatttg gataattctc tcggccttcg acgaagtcag cgcctgcgca
     1981 tcggtattgg cgacgacaaa ttcaacgcca agcaggcctg aaacgatcat gttgttgacc
     2041 gcattgccgc ctgcgccgcc gacgccggcg accataatat gaggttttag ttcccgcagt
     2101 tcgggagctt tgagattgat cgacatcgtc tggcctctcg acgttggagc gctcttcacg
     2161 ctcgggtttg atttatattg gttgcggcaa aacgaaatgt tttgccgcgt ttactcgccg
     2221 cacgcacgct aaaaactagt ccaaagccac tgccctaccc gcccgatata tccgctcgcc
     2281 ttggcctccc ggactagcgg gcgacgcgag gggcggtaat gctcattgcc gctcacttgt
     2341 ggatagatga ggagtccgac agaagccgca aacgctggac tccttgcgga ttcgggcaga
     2401 ccctttatgc caagcggtcg gccgatccgg acctggccgg aaacgattcg ttttaccgcc
     2461 gcttgcatac cggtcagctg acaggctcct cccgtcaaga caagccgccg tcctgcatgc
     2521 gggggaaggc ccgcgttctt caaccggtcg cgcaagagtt caaggatttc ctcgaccctc
     2581 ggcttgatga tggaaatgag ctgcgctttg ggcaaatgcg cgggacgctc ataatcctcg
     2641 cctgccggaa ccaccgcaat tgtctcgctt tcatcggaaa ccgaggcaag gcaggcgcca
     2701 tagagcgtct tcaaccgctc ggcatcggta acggtaattc caagcccgcg cgcgacatcc
     2761 atggtgacat gatgcccgcc gacggcaaaa gaatcgacat gcgtgagatg cccgccggaa
     2821 aacacgctga ccgaggtggt tccggcgccc atgtcaatca aagctgtgcc gagctcggcc
     2881 tcgtcatcgg cgagggcgga tagagccgat gcgtaaggcg ttgcaaccat cgcgtcgacg
     2941 gaaagatggc agcgttcgac cgaaagcagc aaattgcgcg cggccgcggc atcgcagctt
     3001 aagagattca tttccgcgct cagctcgtct ccaaccattc cccgcggatc ttgaatgtga
     3061 cgcgtttcac cgagggaaaa gccattgggc agggaatgta gcaccgccct gctctgttgg
     3121 ctgccgcttg ccgtgcaagc ttcaagaacg cggtggacat cggcttgggt tacggcccgg
     3181 ccagcgattg cgacctttgc ctcataaagc cgcgaggcaa gcctgccgcc tgtcgcattg
     3241 acaatgacgc tttcgacctc gacgccggcc atgcgctctg ccgcatcgac cgcaaaacgg
     3301 atcgccgctt ccgcagcatc catatcgacg acaacaccgc ccttgatgcc gcgcgagcgc
     3361 tgatgtccga taccgaggat ccggcagcta tgggtacgcc cgcgcagcgc ccccgaagca
     3421 tcgctcggat tgagcttggc gaccaggcag acgattttcg atgtgccaat gtcgagcacg
     3481 gaaaggaccg cgcttttctt ggcatgcaaa ggcggcagac gcggcggcag tggccttgaa
     3541 ttcatgtgcg cccgcctttt gccttggctg tacgcggcgg agcgccggcg ctttctcgtg
     3601 ccgccgccgc ttccgcggaa agcctggcga taagacggtc cggctgacgc aggtcgagca
     3661 aaagaacatc cttatcgaga atatgataga ctcgctgaag ctcaatgagc cgcgccatcg
     3721 ctgccgtggg ggctctttcg ggcaacaaaa tctcgagccc gtttgtcaat ttcagcgtcc
     3781 agcggcgccg ggcgacacgc acgccggcca taatgcgttc gcgcaaattc gcggcggatt
     3841 cgagcagcgc caaatattcg ccgatcttgt cattggcccc cgcgcccgcg acgaacggaa
     3901 gatgaataaa gcgccggtcc tggatgaggc ccagaaccac accgtcttcg gctacgatcc
     3961 gcgtctcgcc atcgttctgc caaagggcga atggctgccg ctcctcgact tcgataagaa
     4021 gccggtcggg gtagagtttg gtgatggccg cttccttgac gatcggcaga agtttcagct
     4081 tttctcttat tttggccgcg tcgagaaaca gcaaggaatt gcgcggcccg acgccggcag
     4141 ttgcgagaat gtcctgatct ttgagctcat gcgcgcccga aatcgtcacg gccttgatgg
     4201 aaaagccaag gcctttggcg attatgtcgg ccggttctcc ttcttccgaa acaaatgccg
     4261 catagtggcc gcccatgata gcgccataga cgccaacgac cgtgaagagc gccaaggaaa
     4321 aaaaagccgc ggtgccaggt ttcgccaaaa tgtcgagaat tttttgggta aggccccggc
     4381 gcggcctgcg taccggcggc ctggtctccg ccggcagggt aaaagccgcg ggaaccacgg
     4441 ccccagcgga agatggcgcc gcaggagcaa agccaaagtt cgccgccttt agaacgtgtc
     4501 cagcaaaagt ggaaaccggt tttgcgtccg gacacgcgac aaaacaagga tttaggtcaa
     4561 gatgaccatt cgaagaaaag tcatcttgct ctagcgatcg caggaggcgt cctcgaccat
     4621 ccatcggact agctcaccga atgtgatgcc ggcatagcca gcaagttccg gcacaagcga
     4681 agtctcggtc atcccaggct gggtattgac ttccaaaaca atgagttccc cggttccgcc
     4741 gggcagatcg tcgtaacgga agtctgtccg g
//
LOCUS       DATMPU010000012         3215 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_2178349,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000012 DATMPU010000000
VERSION     DATMPU010000012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 3215)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3215)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3215
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2178349"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..524)
                     /locus_tag="VNY06_00215"
     CDS             complement(<1..524)
                     /locus_tag="VNY06_00215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015685983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX13298.1"
                     /translation="MHVTKTSETQSQSQQLESAAPSKRSSRKSILALSVFALGLNATA
                     AVYTLSPSDFALPNVSLAELLPHQKASDPIPDPVVAALKDIQSAQQQHVASLQENSSL
                     LQQNTALLEQDSTTLASLRESITDEHVDVKKISAQITDEHVDVKKMSAQISTLIAKVD
                     SLKNAITPEVTSSIP"
     gene            complement(828..1133)
                     /locus_tag="VNY06_00220"
     CDS             complement(828..1133)
                     /locus_tag="VNY06_00220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX13299.1"
                     /translation="MTETCKNQRLRDARRDVWRHASALTSYWRARLDWHTALSCAQDR
                     GIADADRLPPADNESRMALVDTWRAALVKQLPTPAPDAGQSHGSGHNSARKATAIRT"
     gene            1708..1974
                     /locus_tag="VNY06_00225"
     CDS             1708..1974
                     /locus_tag="VNY06_00225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018642983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX13300.1"
                     /translation="MNGTAMQFVRVISRVEDMEIWNANRNGFSFVIGYESRSGTGLHG
                     RTGFVASWRPIHQNRCAVRVIGSPFKTLGEAEEACNDMLGLLTT"
     gene            complement(2177..>3215)
                     /locus_tag="VNY06_00230"
     CDS             complement(2177..>3215)
                     /locus_tag="VNY06_00230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX13301.1"
                     /translation="MSAISSPTAPRCGYNDSPRNRSGGRRTPGLAHFSEGDLLRAGEL
                     GHAPLKRGRAAITIRYSSWPVATRAHMIGGWLALFIGIQTLFMTGQPDVARAAIAFPN
                     ATELTAKEIGKDRLSVEHQFGPILRGGLSHRCLEVAEHLAFELRANLIRAENFERKVI
                     GGVHRARFGIEFEKTEIVHDRLQFSGGFPVVFKTIREFRRQQQNIRAFGWSIVDRLPA
                     KHFLQVAFNNEREDLSALRVHEGFGADLGGGSGIYGGGDSVFSVADLMESDYHQADGC
                     EKQSGGRGEQKLSVVGEIARQFHKLSIGFLLFYFAGKLFLGFFGWKYLYDDGRILGSS
                     FLLSGVLLACL"
BASE COUNT          656 a          910 c          948 g          701 t
ORIGIN      
        1 ggaatggagg aagttacttc cggcgttatt gcgtttttca gcgagtcgac cttcgctata
       61 agcgtggaaa tctgagcaga catcttcttc acatcgacat gttcatccgt tatctgagca
      121 gatattttct tcacgtcgac atgttcatcc gtgatgctct ctcttagaga agcgagcgta
      181 gttgaatcct gctcaagcag agctgtgttt tgttgcaaca aggagctatt ctcttgcagc
      241 gatgcaacgt gttgctgctg ggctgactga atgtccttca aagcagcgac aaccggatcc
      301 ggtattggat cggaagcttt ctggtgcgga agcaattcgg ccagactgac attcggcagg
      361 gcaaaatcgg aaggcgacag tgtatagacg gccgctgtgg cgtttaggcc tagagcgaac
      421 accgacagcg ccaagatcga tttccgactg gaccgcttgc tcggtgctgc ggattcgagt
      481 tgttggcttt ggctctgcgt ttccgatgtc ttggtcacgt gcattgacct caaatgagtg
      541 aacaaaaaag gagccgtcgc tggatcgtcc ctcgcgacgg ctcctttacg cggcacccaa
      601 ttaaaactgc cctagattat gggctgatca gattaattcc gcgttagcgc cccagccgcc
      661 ggcgatggct cgccagccgc tgacggttac gcttccggaa tcggcgccgg cgcttcatct
      721 gcgccccggc tttgaagact ggcgcgacgc ggcgatagaa cggcgcgtcg ggtgtgcctc
      781 gaggaacgca gcgtcgtcgg caatcgcgcg ctcgatccgc tctggcttca cgtccgtata
      841 gcggtggctt tccgcgcgga gttgtgcccg cttccatgcg actgcccggc atctggcgcg
      901 ggagtaggta gctgtttcac gagggcggcg cgccacgtat cgacgagcgc catccggctt
      961 tcgttgtcgg ccgggggaag ccggtccgca tccgcaattc cgcggtcctg cgcacacgaa
     1021 agcgcggtat gccaatccag gcgggcgcgc caataagagg tcaatgcgct ggcgtggcgc
     1081 caaacgtcgc ggcgggcatc ccgcaggcgc tggtttttgc aggtttcggt caatggtgcc
     1141 gcaagcggcc gctcgcgcca tattggctct gttggcgcat gatgggcgtt agcgacgatg
     1201 gcgcgggctg gccgcggaaa actgaaatga tttggcagcg ctcatccgcc cctggcggaa
     1261 acattggacg ttcgatggtc atggtggttg ctctcggttt ggcttatcaa ggccgatgcc
     1321 ggtgacggac cggcagccgg gggttgataa ccagccgaga agactggccg aacgctttta
     1381 agggttgccc ctctggacag cacgtccgcc cccggcccaa tatggtcggg ttcggagccg
     1441 cgccgccaag cgcgtccgtt gagcctcaaa gaggctcgtt cagcccctgc ctctcctgcc
     1501 agggaggagg cgaattcctg ccagggaatg cggcctattc tcggtccggg ttatcaagcc
     1561 cacggacacg ctgtcactga tttggttaaa aaacaattcc cctaggttgg ggaaatctgc
     1621 tggcgagcgc tttccgtcca ccgcgccgcc gcgactcagg attggtctgt cttcgggctt
     1681 gggggcttct cgaatgggag acgccccatg aatggaacgg ccatgcagtt cgtccgcgtc
     1741 atcagtcgcg tcgaggacat ggaaatatgg aacgcgaata gaaacggctt ttcgttcgtg
     1801 atcggttatg agagccgcag cggtaccggc cttcacggac gcaccggctt cgtggcgtcg
     1861 tggcggccga tccatcagaa cagatgcgcc gtgagggtta tcggctcgcc gtttaaaacg
     1921 ctcggtgagg ccgaagaagc ctgtaacgac atgctgggcc tcctgacgac atagctatta
     1981 ccgcggtcag tagccaagcc ctcacgggtg gctttcgcgc cttcgcggtt gctctcggcc
     2041 tatagctgcg gttggatgca ggcacggaca tattcggaag ggcgatttac gtatgaggct
     2101 tcgctgccta gtcattgtac tgacattctt agcggcaatg gcgtcggatt cgagaggaca
     2161 gtcaaagcag ccatcctcaa aggcaagcca acaaaacgcc gctcaacaag aacgaggaac
     2221 cgagaattct cccgtcgtcg taaaggtact tccaaccgaa aaatccaagg aagagtttgc
     2281 ccgcgaagta gaaaagcagg aatccgatag acagcttgtg aaattgacgg gcgatctcgc
     2341 caactacact aagcttttgt tcgccgcgac cgccgctttg tttctcgcaa ccatcggcct
     2401 ggtggtagtc ggattccatc aggtccgcga cgctaaacac gctatcgccg ccgccgtaaa
     2461 ttccgctgcc gccgccaaga tcagcgccga agccttcatg aacgcggagg gcgctcaaat
     2521 cttcccgctc gttattgaaa gcaacttgaa ggaagtgttt agcgggcaat cgatctacta
     2581 tactccagcc gaaagcccgg atgttctgtt gctgcctcct aaactctctt attgttttaa
     2641 aaactacggg aaaacccccg ctaaattgca atcgatcatg cacaatctca gttttctcga
     2701 actcaattcc aaatcgcgcc ctatgcacgc cgccgatgac tttccgctcg aaattctcgg
     2761 cgcggataag gttagctcta agctcgaatg cgagatgctc ggccacttca aggcaccgat
     2821 ggctcaagcc gccgcggagt atagggccga attgatgttc tacggagagg cggtctttac
     2881 cgatttcttt ggccgtcaat tccgttgcat ttgggaatgc gattgccgct ctggcgacgt
     2941 caggctgacc cgtcatgaac aacgtttgga tcccgataaa aagagctaac cagccgccga
     3001 tcatgtgcgc acgggtggcc acgggccatg agctgtagcg gattgttata gcagcgcggc
     3061 cgcgcttcaa tggcgcatgc ccgagctcac ccgcccgcag aagatcacct tcggagaaat
     3121 gcgcgaggcc tggcgtgcgg cggcctcctg atctattgcg cggactatca ttgtagccac
     3181 agcgtggcgc ggttggcgac gaaatcgcgc tcatg
//
LOCUS       DATMPU010000013         2418 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_4348970,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000013 DATMPU010000000
VERSION     DATMPU010000013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 2418)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2418)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2418
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4348970"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..318)
                     /locus_tag="VNY06_00235"
     CDS             complement(<1..318)
                     /locus_tag="VNY06_00235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009736861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HWX13302.1"
                     /translation="MTDFVIDVHNLQKSFATRKVVDGLTLEVASGEICGFLGANGSGK
                     TTTIRMLCGLLVPDGGSGKCLGHDIIREASLIRREAGYMTQKFSLYEDLTVFENLDLV
                     ASVY"
     gene            complement(315..1307)
                     /locus_tag="VNY06_00240"
     CDS             complement(315..1307)
                     /locus_tag="VNY06_00240"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HWX13303.1"
                     /translation="MNRRVALAVALAALAASAGFWWSRVRNPAPVEWQGYAEADYVKV
                     GPTLEGLLTSVFVARGTKVAAGAPLFDQDDIADRAATDQATRQLNQAEAQLANLQSAG
                     KPTEIGQAEANLADAVAARDKISADLQRTETLLKSGKASEQLADQRRADLRSAEAKIK
                     AFEAALAQMRNPLGRESEIKAQQAAVEAARAAQAMAQWRFDQRHVAAPATGVVMDVLA
                     RPGETMPAAVPVVSLLPPENIFVRFFVPEAMLAQIHYGDQVRIICDNCPAELWATVSF
                     ISPQAEYTPPVIYSESSRAKLVFKVEARPSQQQAPLLNPGQPVAVRPVPRDGAK"
     gene            1387..2043
                     /locus_tag="VNY06_00245"
     CDS             1387..2043
                     /locus_tag="VNY06_00245"
                     /inference="COORDINATES: protein motif:HMM:NF025605.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HWX13304.1"
                     /translation="MQNEALEPSAFKRGQGGRPTRQEAERRHLSLLAAAARLFLKQGW
                     DGTSIDEISRQSGVAKRFIYARYPDKAALFVGALQRYLDQQIGILHQVEPPPDDVEEG
                     LCCLGERLLDIALQPETLAFQRLLIAEAPRFPALAKLFVERNRRRVVAEISNVLEGYA
                     SRRVIQFGDLEVAAEQFFILIVGIPQRLALLAGRDAIAKDEQRIKAAVRLFLDGCRQH
                     "
     gene            complement(2220..>2418)
                     /locus_tag="VNY06_00250"
     CDS             complement(2220..>2418)
                     /locus_tag="VNY06_00250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016920049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HWX13305.1"
                     /translation="KVEYLYYDIGSVSYALSPLVDNSLVTGTALSTAFARSSTRFNGN
                     IVRAGINYHFNWTPAPVVAKY"
BASE COUNT          484 a          702 c          743 g          489 t
ORIGIN      
        1 gtagacgctc gcgaccaagt cgaggttttc aaacaccgtg aggtcctcat agaggctgaa
       61 cttttgcgtc atgtagccgg cctcgcggcg gatcaacgag gcctcgcgta tgatgtcgtg
      121 tccaaggcac ttgccgcttc cgccatcggg gacaagcagg ccgcacagca tccggatggt
      181 cgtggttttg ccgctgccgt tggcgcccaa aaagccgcag atttcgccgc tggccacttc
      241 caaagtaagc ccatcgacaa ccttgcgggt tgcaaaactc ttctgcaaat tatggacgtc
      301 aatgacgaag tccgtcattt tgcgccatcc cttggaaccg gcctcacggc aaccggctga
      361 cccggattga gaagcggagc ttgttgctgg gaaggcctcg cctcgacctt gaagacaagc
      421 ttggcgcggc tcgattcgct atagatgacc gggggcgtat attcggcctg cggcgagatg
      481 aaggaaaccg ttgcccaaag ctctgccggg caattgtcgc agataatccg aacttggtcg
      541 ccataatgta tctgtgcgag catcgcttct ggcacgaaaa atctcacaaa aatgttttcg
      601 ggcggcaata aggacacgac cggaactgcg gcgggcattg tttcgcctgg gcgggcgagc
      661 acgtccatca cgacgccagt tgcgggagct gcaacatggc gctggtcgaa ccgccactgc
      721 gccatggcct gagccgcacg cgcggcctcc acagcggcct gctgggcctt gatctcggat
      781 tcacggccaa gggggttgcg catctgcgcc aaggcggcct caaaagcttt gatttttgcc
      841 tctgcggacc gcaaatccgc gcgccgctga tccgcgagct gctccgatgc tttgcctgac
      901 ttcagtagcg tttcggtccg ctggaggtcg gccgagatct tgtcacgtgc cgcaaccgca
      961 tcggcgagat tcgcctcggc ctgcccgatc tcggtgggtt tgccggcaga ctggagattt
     1021 gcgagttgcg cttccgcttg gttcagttgg cgagtggcct gatccgtggc tgcccggtcc
     1081 gcgatgtcgt cttgatcgaa gagcggcgct ccagccgcga ctttagttcc tcgcgctacg
     1141 aatacggaag tgaggagtcc ttcaagggtg gggccgacct tcacgtaatc ggcctcggca
     1201 tagccttgcc actcgacagg cgcaggattg cggacccgcg accaccagaa accggcagag
     1261 gccgccagcg ccgcaagcgc caccgcaaga gcgacacgcc gattcaccgg cttgaccttt
     1321 cgcaacgaga atgtatcgtt gcgttatcag tttcggcctc taccggcaag gggaaggact
     1381 tttcaggtgc aaaatgaagc gctcgagcca agcgctttca agcgcggcca gggcggccgg
     1441 ccgacgcggc aggaggccga gcggcggcat ttgagcctgc tcgctgctgc agcccggctg
     1501 ttcttgaagc agggctggga tggcacgtcg attgacgaaa tttcccgcca atccggggtc
     1561 gcgaagcgct tcatttatgc gcgctacccc gacaaggcgg cgcttttcgt cggcgcgctc
     1621 cagcgatatc tcgaccagca gatcgggatc ttgcaccaag ttgagccacc gccggatgac
     1681 gtcgaagaag ggctctgctg ccttggggaa aggctcttgg acatcgcgct tcagcccgaa
     1741 acgcttgcct ttcagcgcct cctcattgcc gaggccccca gattcccggc tcttgcaaag
     1801 ctttttgtcg aacgcaatcg ccgccgggtt gttgctgaaa tcagcaatgt cctcgaggga
     1861 tacgctagtc gccgggtgat ccaattcggc gatcttgagg tcgccgccga gcaattcttc
     1921 atccttattg ttggtatacc tcagcgcttg gctttgctcg ccgggcgtga cgcgatcgcg
     1981 aaagacgagc agcggatcaa agcggcggtg cggctattcc tggacgggtg ccgccaacat
     2041 tgagcagcgg ccccgagcga accgatgaca cgaaaacgta ggctgcgaag agccggtttg
     2101 catcggcgaa agggcaatgg aaagcgcaag gctacgaaat tatgttcgcg cacatacaaa
     2161 aacccgaccg ctggggccgg gtttgagttt ggttggcaga aacgattctg gccgagattt
     2221 tagtattttg cgacgacggg cgcgggcgtc caattgaagt gatagttgat gccggcgcgg
     2281 acgatattgc cgttaaaccg agtcgaggaa cgcgcaaagg cggtgctcaa agcagtacca
     2341 gtgacaaggg agttatcgac gagcgggctc agggcataac tcacgctgcc gatatcgtaa
     2401 tagagatact cgactttt
//
LOCUS       DATMPU010000014         6530 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_1249627,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000014 DATMPU010000000
VERSION     DATMPU010000014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 6530)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6530)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6530
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1249627"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..638)
                     /locus_tag="VNY06_00255"
     CDS             complement(<1..638)
                     /locus_tag="VNY06_00255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009763445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX13306.1"
                     /translation="MTLFRRTLLSLAASPLCLVIASIAPHAEGASVPERRIDQVEAQK
                     AETQNTISIAQGYKRPPGEIGEGGDALYPEDQSDGGPQDSAGLLIRLGRLESQMRQIN
                     GQIEQMQFETRRLEEQLKKFQEDVEFRFRDGSQRVPGANPSQRRGEALQPRIHGDYSA
                     EPPAQHAAVTAPRANGRGDAFDPTQAPDAPGAPRSLGSSAANRSVQGLTDNG"
     gene            complement(1021..1893)
                     /locus_tag="VNY06_00260"
     CDS             complement(1021..1893)
                     /locus_tag="VNY06_00260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012384635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChbG/HpnK family deacetylase"
                     /protein_id="HWX13307.1"
                     /translation="MGTAKAQFSFSLCADDYALSPGVSRGILEALCASRLTATGVVAT
                     SPSWPSGARALREYRAKADIGLHLNLTHGSPLGSMPAFAPSRYFPKLGRVLKTALIGE
                     LPQAEIGEEISRQIDKFCEHFGAAPDFVDGHCHVQILPQVRRPLFKTLGEKGLSGKVW
                     LRNSSDSLLRILRRNAGEFKKALGIAWLGTRFAKEASARGFTTNDGFAGFSAFDVGRD
                     YAADFARYLRAPGRRHLIMCHPGYCDEELVAADPATLSREQELRFLLSSDFIEILDHC
                     GAMLTRFSDHLARN"
     gene            2155..3153
                     /locus_tag="VNY06_00265"
     CDS             2155..3153
                     /locus_tag="VNY06_00265"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003613571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HWX13308.1"
                     /translation="MSISRAQGAGVELSVITPAFNESKNIDAMLKRLVPVLTRCVASF
                     EVIFIDDGSTDDTLERLRAANQEDSRVRALSLSRNFGKEIAIAAGLDHAAGAATVIMD
                     ADLQHPPELIETFVARWRDGYKNVFAQQIGHNLYSPLRRILTQRFYRLFGSVGETRLP
                     EGAGDFRLLDSQAVAALQKMREHARFSKGLYAWIGFKSIGVPFDAEPRLYGTTKFSYR
                     RLTHLALDGLMSFSTIPLRVWTYIGLATSVLALTLAAFYLVRTLIFGVDVPGYASLMV
                     SITFFAGVQLLSLGIIGEYIGRIFGEVKRRPLYLIEERVGFSVLAEENSFARLPSG"
     gene            3167..4702
                     /gene="murJ"
                     /locus_tag="VNY06_00270"
     CDS             3167..4702
                     /gene="murJ"
                     /locus_tag="VNY06_00270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012589418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HWX13309.1"
                     /translation="MYKSLFSVGGLTLLSRGAGFVRDILLGAFLGAGLLADAFVVAQR
                     LPNHFRAIFGEGAFNSAYVPSYAKVLEVEGLERARLFAGQIFLGLFASQIFVLVLALG
                     FTPYLVDLLAPGFREDPRKFALTITLTQITFPYLLFISLVTLQSGTLNAHGYFAAAAF
                     APVVMNLTMIVFLGLAFLFPNPGLAASWGLTVSGVLQLALTSLAAHRSGIFERFARAP
                     MMQHIKRFLVTLVPAVIGSAGIQIALFADTIIGSLLPTGGVSSIYYADRIYQLPLGVI
                     GIAAGTVLLPEMSRFFAAGNASAALHAQNRTMALSLALTAPFFIAFIMIPEAIMRGIF
                     LRGAFTVDAANASAAVLAAYGFGLIAIVLLRPAVASFQAQGDTRTPMTVALFAVAINV
                     LLKILLFKPFGAPGLAAATGVGAWVNLALLAFLAIRRKAMRIDLFFWKMATAITIASF
                     ILAVFALFGAAPAQRLAGGLGAFANLTELLLLGIAGALVYGFALLAGLRIAKVNLWLP
                     RPS"
     gene            complement(4723..6105)
                     /locus_tag="VNY06_00275"
     CDS             complement(4723..6105)
                     /locus_tag="VNY06_00275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012589415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HWX13310.1"
                     /translation="MAALAEVLEKIDANLELALERLFRLIEIPSVSTDPAYAGDCARA
                     AEWLAAELRSIGFEASVRETSGHPMVVGNAQAARPDVPHLLLYGHYDVQPPDPLDLWE
                     SPPFEPRIVEAATGKRIVGRGVADDKGQLMTFLEACRAFHETGGLPCHITFLIEGEEE
                     TGSPSLPAFLDKAKDELIADMALVCDSLMWDRETPAVTTMLRGLVLEEVVIHAADRDL
                     HSGMFGSAAVNPIHVLARVIADLRDGKGHITLPGFYNGVAELPHDTAEQWCNLPFDES
                     AFLGQVGLSVPAGEQGRSVLEMIWSRPTCDVNGIVGGYTGKGSKTVLPAQASAKFSFR
                     LVGAQDPRKITQSFRAFVKERLPADCRAEFVSHGASSAMQLPFSSEALSRASRALQAE
                     WGKEPVLAGCGGSIPIVGAFKRDLGMDSLMIGFGLEDDRIHSPNEKYELSSFQKGARS
                     WARVLNALAL"
     gene            6309..>6530
                     /locus_tag="VNY06_00280"
     CDS             6309..>6530
                     /locus_tag="VNY06_00280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012589774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HWX13311.1"
                     /translation="MEIDTENPAGLRAPLPGILSRLEAIVGAKHVIADTSGMEGFLRE
                     PRGRFHGQALCVVQPGSTQEVAAILRLCNE"
BASE COUNT         1244 a         1897 c         1860 g         1529 t
ORIGIN      
        1 tgaccgttgt cggtgagccc ctgaactgag cgattggcgg cagagcttcc gagtgaccgc
       61 ggagctcctg gggcatccgg agcttgcgtg gggtcaaagg catcgccgcg tccgttcgcg
      121 cgcggagccg tgaccgcagc atgttgcgcc ggaggctccg ccgagtagtc gccatgaatt
      181 cgcggttgca aagcttcgcc cctccgctga gagggattgg ccccaggaac acgctgcgag
      241 ccgtcgcgaa accggaattc gacatcctct tgaaatttct tgagctgttc ctcaagcctg
      301 cgggtttcga actgcatctg ttcgatctgc ccgttgatct gtcgcatttg gctttcgagc
      361 cggcctagcc ggatcagcag tcccgcggag tcctgtggcc ctccatccga ctgatcctcg
      421 gggtaaaggg cgtccccgcc ttctccgatc tcgcccggcg ggcgcttata tccttgcgcg
      481 atcgatatcg tattttgtgt ttccgccttc tgcgcttcga cctggtcaat gcgcctctcc
      541 ggaacgctcg cgccctccgc atgtggcgcg atcgaggcga tcaccaagca aagaggcgat
      601 gcagccagcg aaagtaaggt tctgcgaaat aacgtcatat gacaatgtcc tatgggccag
      661 ggcctccatt gtaggcggga tttttaggcc aaaattggga gggcctggcg cttgcgccgg
      721 aaatgagcgt tgtcctacta gatagtgcaa gtctttggga agggcagcct gcggcgtccc
      781 gccgcctctt gccggaatcg ccgtctgcgc caaggagagc aatatgcaag gcccggtggc
      841 gccagagggc tgcgggtggc tagagcaaga tgacttttct tcgaatcgtc atcttgctct
      901 aatgccttgg tttgtcgcgt gtccgggcgc aaaaaccggt tccacttttg gctcgacacg
      961 ctcaaggtgc tgaaaaagta accgcctgct gcggtatgga accaagcgtt gcgaggcgac
     1021 tcaatttcgc gcgaggtgat cagaaaaccg cgtaagcatg gcgccgcagt gatccagtat
     1081 ctcaatgaaa tcggaggaca agaggaaacg gagttcttgt tcgcggctga gcgtggctgg
     1141 atcagcagcg acgagctctt catcgcaata acccggatga cacatgatga gatggcgcct
     1201 acctggcgct cggagataac gtgcgaaatc agcagcataa tcacgcccaa catcaaaggc
     1261 ggagaaaccg gcaaagccgt cgtttgtagt gaagccgcgg gcggaggctt ccttggcaaa
     1321 ccgcgtccca agccaggcga tgcccaaagc tttcttaaat tcgcccgcat tccgccgcag
     1381 aatccttagc aagctgtcgg aactgttgcg cagccacacc ttgcccgaga gacctttttc
     1441 tccaagtgtt ttgaacaacg gcctgcggac ctggggcagg atctggacgt ggcaatgtcc
     1501 atccacaaaa tcgggagcgg caccgaaatg ctcgcaaaat ttgtcgatct ggcgcgaaat
     1561 ttcctccccg atctcggctt gcggtaattc cccgatcagc gcggttttga ggactctgcc
     1621 gagttttgga aagtacctag aaggagcaaa cgccggcatg gatccaaggg ggctgccatg
     1681 ggtgagattg aggtggagcc cgatgtcggc ttttgcacgg tattcgcgca gggcccgcgc
     1741 gcccgagggc catgatggac ttgtcgcgac gacgcctgtc gcggtcaaac ggctcgcgca
     1801 aagcgcttcg aggatacctc ggcttacccc cggcgacagc gcgtagtcgt cggcacagag
     1861 gctgaaggaa aattgcgctt tcgctgtgcc catattggtc cgttctatcg ccgcggaaaa
     1921 aatgcgccga ggctggcagc gtttggcgcg gcctcagcca tgcgtctaag agctaggagg
     1981 gacttttctt cgaatcgttc tattactcta attctggttt tgacgtgtgt ccggacacaa
     2041 ggccggcttt tacttttgtt caacacgctc tagcgatctt gtccaaggct aaccgaaacc
     2101 ttaagtcatt tgcccgggcg aggtccgtgg gtttgcttta tgaggccgcc acaaatgtca
     2161 atttccaggg ctcaaggtgc aggcgtggag ctttccgtga tcacgcccgc cttcaatgaa
     2221 tcaaagaaca tagatgcgat gttaaagagg ctcgtgccgg tcctcacgcg ctgtgtggcc
     2281 tcatttgaag ttattttcat cgatgacggt tccacggacg atacgctcga gcggctgcgc
     2341 gccgcgaatc aggaagactc gcgcgtccgt gcgctctcgc taagccggaa ttttggcaag
     2401 gagattgcga tcgcggcggg gctcgaccat gcagcagggg cggcgactgt catcatggat
     2461 gccgatctgc aacatccccc ggaattgatt gagacctttg ttgcccgctg gcgcgatggc
     2521 tacaaaaatg tgttcgcgca gcagatcggt cacaacctct acagcccctt gcggcgcatc
     2581 ttgacgcagc ggttttacag gctcttcggc tcggttggag agacgcgctt gccagagggt
     2641 gccggagatt tcaggctgct tgattcacag gccgtcgctg ccctgcagaa gatgcgagaa
     2701 cacgcccgtt tttccaaagg gctctatgcc tggatcggct tcaaatcgat tggcgtgccg
     2761 ttcgatgccg agccgcggct ttacggtaca accaagttca gttatcgcag attaacgcat
     2821 ttggcccttg atgggctcat gtcgttttcg acaatcccgc taagagtgtg gacttatatt
     2881 ggcttggcta catcggtgct ggcgctaaca ttggcggcct tttatctcgt tcgtactctc
     2941 attttcggtg tcgacgttcc gggttacgcg tccttgatgg tatcgattac cttttttgct
     3001 ggagttcagt tgctgtcgct tgggatcata ggagaatata ttggccgcat cttcggcgaa
     3061 gtaaaacggc ggcccctcta tctgattgaa gagcgcgtgg ggttcagcgt gttggccgaa
     3121 gagaattcat tcgctcgact gccttccggg taaatcgaaa ccgaatatgt acaaaagcct
     3181 gttctccgtc ggcggcttga ctttgctttc gcgcggcgcc ggctttgtgc gcgacattct
     3241 gcttggcgct tttctgggcg ctggactgct tgccgacgca tttgtggtgg cccagcgcct
     3301 gcccaatcat tttcgtgcca tttttgggga aggcgccttc aattctgctt atgttccaag
     3361 ttacgcgaaa gtgctcgagg tagaaggcct tgaacgggcg aggctttttg ccgggcagat
     3421 ctttttgggc ctctttgcct ctcagatttt tgttcttgtg ctcgctctcg gctttacccc
     3481 ctatctcgtc gaccttctcg cgcccgggtt ccgcgaagat ccgcgaaaat tcgcgctcac
     3541 cattaccctg acgcagatta cctttcccta tctcctgttc atttctctgg tgacccttca
     3601 gtctggcacc ttgaacgcgc atggctattt cgccgccgca gcctttgcgc ctgtcgtgat
     3661 gaatctcacg atgatcgttt tcctcggcct tgcctttttg tttcccaatc ccggcttggc
     3721 ggcgagttgg gggctcaccg tgtcaggcgt attgcagctt gctctcacct ctttggctgc
     3781 ccaccgctca ggcattttcg agcgtttcgc gcgcgcgccg atgatgcaac acatcaagcg
     3841 attcttggtg acattggtgc cggcggtaat cggctcagca ggcattcaga tcgcgctttt
     3901 cgccgacacg attatcggct ccttgctgcc gacaggcggc gtttcttcca tctattatgc
     3961 cgaccggatc taccagctgc cgcttggcgt cattggaatt gcagccggca ctgtgctgtt
     4021 gccggaaatg agccgattct ttgccgcggg caatgcctct gccgcattgc atgcgcagaa
     4081 tcgaaccatg gcgctctcgc ttgccctgac agcgccgttc ttcattgctt tcatcatgat
     4141 tcctgaagct atcatgcgtg gaattttcct gcgcggcgcc ttcacggtgg acgcagccaa
     4201 tgcctctgcc gctgtgctcg cggcttatgg cttcggtctt atcgctattg ttctattgcg
     4261 tccggccgtg gcgagtttcc aagctcaggg cgacacaagg acgccgatga cggtcgctct
     4321 cttcgctgtc gcgatcaatg ttctcttaaa aattttactg ttcaagcctt ttggcgctcc
     4381 cgggcttgcc gcggcaaccg gggtcggcgc ttgggttaac cttgccctgt tggctttcct
     4441 ggcgattagg cgcaaagcca tgcgcatcga tctcttcttt tggaagatgg ctaccgcaat
     4501 aactatcgct tcctttattt tagcggtttt tgcactcttt ggtgctgcgc ccgcccaacg
     4561 cctcgctggc gggcttggcg cctttgcaaa tctcactgag ctcctgcttc tcgggattgc
     4621 cggcgctctc gtctatgggt ttgctctcct tgctggactg cggatcgcca aggtgaatct
     4681 ttggttgccg cggccctcct gacggccccc ttgcgaaagg cgttacaagg ctagcgcatt
     4741 taaaacccgt gcccagcttc tcgcgccctt ttggaacgat gaaagctcgt atttttcgtt
     4801 cggcgagtga atgcgatcgt cttcgaggcc aaagccgatc atcaaactat ccattccaag
     4861 gtctcgtttg aaggcgccga caattgggat cgacccgccg caaccggcga gaaccggctc
     4921 tttgccccat tcggcctgca acgcccgaga cgctcgcgac agggcctcgg aactgaatgg
     4981 aagctgcatg gcgcttgacg cgccgtgcga aacaaattcg gcgcggcaat cggcgggcaa
     5041 gcgttccttg acgaaggccc ggaaggattg ggtgatcttt cgcggatctt gtgcgccaac
     5101 aagccggaag gaaaatttgg cgctcgcttg cgccggaaga acggttttcg acccctttcc
     5161 cgtatagccg ccaacgatcc cgttgacatc gcaagttggc ctcgaccaaa tcatttcgag
     5221 cacgctgcgg ccttgttcgc ccgccgggac cgaaagccca acctggccaa gaaaggcgct
     5281 ttcgtcgaac ggcaaattgc accactgttc ggcggtgtca tgtggcagct cggcgacccc
     5341 attatagaag ccgggaagcg tgatgtgccc tttcccatcg cgcagatcgg caatgacgcg
     5401 cgcaagcaca tgaatggggt taaccgcagc actgccaaac atccccgaat ggagatcgcg
     5461 atcggcggca tggatcacaa cctcctcaag cacaaggccg cgcagcatgg tggtaaccgc
     5521 cggggtctcc cggtcccaca tcaagctgtc gcagacaagc gccatatcgg ctatgagttc
     5581 gtccttggct ttgtcgagaa aagccggcag ggacggcgac ccagtttcct cctccccttc
     5641 tatgagaaag gtaatgtggc agggcaggcc gccagtctca tggaacgcgc ggcaggcttc
     5701 gaggaaagtc atgagctgac ccttatcgtc agccactccg cgcccgacaa tccgcttgcc
     5761 tgttgcggcc tcgacaatgc gcggctcgaa aggggggctc tcccataaat cgagcggatc
     5821 gggcggctgc acgtcataat ggccgtacaa gagcagatgc gggacatccg ggcgcgcggc
     5881 ctgggcattg ccgacgacca tcggatgccc agaagtttcg cgcaccgacg cctcaaatcc
     5941 gatgctccgt agctcggcag cgagccattc cgccgccctc gcgcagtcgc cagcataagc
     6001 ggggtcggtt gaaaccgacg ggatctcgat gagccgaaaa agccgttcca gagccaattc
     6061 cagattggca tcgatcttct ccagaacctc tgcgagcgcg gccataggac ctccttgtat
     6121 tctattcctc acaaaggatg cagcgttccc caacgcctca acagagcggt ggagcgccga
     6181 attataaccc gctttgagag tttatgttgt ttcaatcgcc aattgggcca ataatttcac
     6241 ctggagggcg gaaatgccgc catctgccca aaatctgccc cgcgcatgat tacattggat
     6301 gctatgagat ggaaattgat accgaaaatc cggcgggttt gcgcgctcca ttgccgggca
     6361 tcctttcccg tctcgaagca attgttggcg ccaaacatgt catcgccgac acctccggca
     6421 tggaagggtt tttgcgcgag ccccgcggcc gctttcatgg acaagcactt tgcgtggtgc
     6481 agccgggctc aacgcaagaa gttgcggcca ttctcagact ctgcaacgag
//
LOCUS       DATMPU010000015         2818 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_1250030,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000015 DATMPU010000000
VERSION     DATMPU010000015.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 2818)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2818)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2818
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1250030"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..521
                     /locus_tag="VNY06_00285"
     CDS             <1..521
                     /locus_tag="VNY06_00285"
                     /inference="COORDINATES: protein motif:HMM:NF037152.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HWX13312.1"
                     /translation="FQKTTVADIARELRMSPANVYRFFGAKSEINEAVCMEILGKIEA
                     EAGKIAVSRGTALQRMRNLVASVEKAFYKQYMLDRKLHELIEAAVTENWTTMRAHNHR
                     MNLMLEQIIASGMAAGEFHPGDAALAARLVNTACIRFCHPRLMAEYEQEPEPALDHML
                     DFCRAALVREPV"
     gene            complement(877..1884)
                     /locus_tag="VNY06_00290"
     CDS             complement(877..1884)
                     /locus_tag="VNY06_00290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020174325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HWX13313.1"
                     /translation="MRILVTGSAGFIGFHMARRLLDEGHEVAGIDGFTPYYDRNLKLR
                     RHEILLQRPGYAGHEAMLEDRAALQRIYGDGFDAVYHFAAQAEVRYSLDNPRAYVDAN
                     LTGTFNLLELMRHAPPAHALLASTSSVYGANAKVPFREIGRADHPLTLYAASKKANEE
                     MAHAYSHLFKIPITMLRFFTVYGPWGRPDMALFKFAAALFEGRPIDVYNHGKMSRDFT
                     YIDDLVEAMLRLIGHAPAQVEGRAAPLPVDSLSPVAPWRVVNIGAETPVALMDFVAAV
                     EAATGVRAKCNFMAMQQGDVPDTFADTSLLLALTGFAPKTQLKEGVKAFIAWYHDYFG
                     F"
     gene            complement(2356..2706)
                     /locus_tag="VNY06_00295"
     CDS             complement(2356..2706)
                     /locus_tag="VNY06_00295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX13314.1"
                     /translation="MPPDQYLACVNGAKNYEPKIVQVSNVIIEAEKAKQEGNSPLAEQ
                     KAAEAKSAVAASAGIVTDLDKLAQAAPAARLAHSIDNPRKQRPLSSLKIVKPFQWMQK
                     RFQPIPTGEVTGYT"
BASE COUNT          615 a          807 c          782 g          614 t
ORIGIN      
        1 gtttccaaaa gacgacggtc gccgatatcg cacgcgagct gcgcatgtct ccggcgaatg
       61 tctatcgctt cttcggggca aaatccgaaa tcaacgaggc tgtctgcatg gaaatcctcg
      121 gcaagatcga agccgaggct ggaaaaatcg ccgtgtctcg cggcaccgcc ctccaaagaa
      181 tgcgaaactt ggtcgcgtcc gtcgaaaagg cattttacaa acagtacatg ttggaccgaa
      241 agctgcacga gttgatcgaa gccgcggtga ccgaaaactg gacgaccatg cgcgcgcaca
      301 accatcgcat gaatttgatg ctcgaacaga taattgccag cggcatggcg gcaggcgagt
      361 tccatccggg agacgcggcg ctcgcggcgc gccttgtcaa caccgcttgc atccgcttct
      421 gtcatccgcg cctcatggcg gaatatgagc aggagccgga accggctctt gatcacatgc
      481 tcgatttttg ccgggccgcg ctcgttcggg agcccgtttg aaccggttgc cgcataaaaa
      541 gggccgttcc gtcgcaattt gactggccgg ccagcgcggg gcaggcggtg catgacggcc
      601 gaaccggtct tcaatcaagc cagttaatcg tcgcggatcg aggaagtcgc tctcccgctc
      661 tcgcttcgcg cctcgccggc ggcagcccat cttcgtcgaa accgacgcaa aagtcataag
      721 acaagaatgt cccgaaaata gctcggcttt ttcgattcca gcctgctcca actcaacgat
      781 tttgaactcc gcctttttcg atcgaatggt tccatttatc gggacgcgct ctaagcacgg
      841 gccaaagccc gaccattgtc attgctacgc cttgtctcaa aaaccgaaat aatcatggta
      901 ccacgcaatg aaagccttaa ctccttcttt cagctgcgtt tttggggcaa aaccagtcag
      961 agccaaaagc aacgaagtgt cggcgaaggt gtctggaaca tcgccttgct gcatcgccat
     1021 gaaattgcac ttggcccgta ctccggtcgc ggcctcgacg gcggcgacga aatccatcag
     1081 cgcgaccggt gtctcggcac cgatgttgac gacccgccaa ggcgcgacgg gggataggct
     1141 gtcaacggga aggggcgcgg ctcttccttc gacctgggcc ggcgcatggc caatgagccg
     1201 gagcatcgct tcgacgagat cgtcgatata ggtaaaatcc cggctcattt tcccatgatt
     1261 atagacatcg attgggcgac cctcgaatag cgcggcggca aatttgaaaa gcgccatgtc
     1321 cgggcggccc caggggccgt agaccgtgaa aaatcgcaac atcgtgatcg ggattttgaa
     1381 gagatggcta tacgcatggg ccatctcctc gttcgccttt ttcgaggcgg catagagcgt
     1441 caacgggtga tcggcccggc cgatctcgcg aaatgggacc ttcgcgttgg ccccatagac
     1501 cgaggatgtc gaagccagca gggcatgcgc cggtggcgca tggcgcatca actctaggag
     1561 attgaaggtt ccggtcagat tggcgtcgac ataggcgcgc ggattgtcga ggctgtagcg
     1621 cacctccgcc tgcgcggcga aatggtagac cgcatcaaac ccgtcgccat aaatcctttg
     1681 caatgccgcg cgatcctcga gcatcgcctc gtggccggca tagcccggcc tttgcagcaa
     1741 tatctcgtgc cggcgtagct tgaggttgcg gtcgtaatag ggcgtaaacc catcgatgcc
     1801 ggcgacttca tggccttcgt cgagaagacg gcgagccatg tgaaaaccta tgaagccggc
     1861 gctgccggtg acgagaatac gcatcgttcg cgcgatcgcg gagaaggggt ttaacggccc
     1921 gcgcgaacct cgttaatgcc ttgggcaccc gcggagaaga aaatacgtcg cataaatgcc
     1981 ccgcgattac cacgttgacg gccgagctct cctttgcggt atcaacttat atcggcacaa
     2041 acatgcacct cggtctttca gatgccggca aatttcctcg ataggcaatt gcaagaccga
     2101 gatcgctgtc agccaactcg cggggtagcg atctgttgag ccgggctctc aacggccgaa
     2161 gtccacgcgc tcgatggtgc ggatatttgc ttcgaatggg tcaaactcat gtttgtccta
     2221 ataatacaat cggaactaac gtggcctgtc aaatgttccc cgttaagagg tacaaattct
     2281 caaattcaat tgttggcgtt tcatcaaaca cggaaaaact ttgcgtattc ttaaatagaa
     2341 ttttatgccc gtaatctaag tgtagcccgt cacctcccca gtcggtatag gttgaaaccg
     2401 tttttgcatc cactgaaacg gttttactat ttttaagctt gataacggcc tttgcttccg
     2461 tgggttatca atagagtgtg ccagtcgagc ggctggcgcg gcctgcgcca gcttgtcgag
     2521 atcggtcacg atgcccgcgg aagccgcaac ggccgatttc gcttccgcgg ctttctgctc
     2581 tgcaagcgga gagtttcctt cctgcttggc tttctccgct tctataatga cgttcgaaac
     2641 ctgcacgatc tttggttcat agtttttcgc cccgttgaca caggccagat actgatccgg
     2701 cggcaaggga ggctcgctgt gctgcgcctg gcaacacgcc gtgagatgct cgccgacttt
     2761 tgcgcctcgg tcatttgcgt gcccgccgac agtcgtcggg attcaaatcc taacccaa
//
LOCUS       DATMPU010000016         4999 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_2181763,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000016 DATMPU010000000
VERSION     DATMPU010000016.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 4999)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4999)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4999
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2181763"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..139
                     /locus_tag="VNY06_00300"
     CDS             <1..139
                     /locus_tag="VNY06_00300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013215506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="methylenetetrahydromethanopterin dehydrogenase"
                     /protein_id="HWX13315.1"
                     /translation="LGLGPLAIGDVKYKTESGLFQRMASSSKAVSFDFRDAFALAREI
                     V"
     gene            183..1289
                     /locus_tag="VNY06_00305"
     CDS             183..1289
                     /locus_tag="VNY06_00305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012591385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HWX13316.1"
                     /translation="MAAAARRAGYRPLALDFFGDIDTREVCAANRRVAGGLEKGFAEE
                     NLIPALKALAKGEDDCGVVYGAGFEDRPGLLDCLARHWTLLGNLSDVVRRVKDPVKLA
                     ELCAGLNIAHPEISARRPMEHHQWLVKSVGGSGGMHVAPASAAWAEGEGIYFQRKAEG
                     EPISIQFLADGHNTQVIGLSRRWVAPAPGEPFRFGGVLRPANVSPKMDRQLRQTAAAV
                     TAACGLLGLNTIDFLVKNNAYTLIEINPRPGAALDIFEDPKGSLFHAHVNACMGRLPA
                     YPLEFTGAAAAAIAYAPRAIASLPDFNWPEWTADRQKAKSALRAHDPLCTIKATAGEP
                     LSARALIETRTNLLLDRLAHIQNQNSSNGKETTL"
     gene            1286..2254
                     /gene="mch"
                     /locus_tag="VNY06_00310"
     CDS             1286..2254
                     /gene="mch"
                     /locus_tag="VNY06_00310"
                     /EC_number="3.5.4.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009210867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018759 - methenyltetrahydromethanopterin
                     cyclohydrolase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methenyltetrahydromethanopterin cyclohydrolase"
                     /protein_id="HWX13317.1"
                     /translation="MSKSELSINTLTGQAVDRLIANAARLRLTVAKGEAGETVIDAGQ
                     NAHGGIEAGLAIAEICLGGLGTVGLAPTGINPKWPFELCVRSTDPVTACLASQYAGWA
                     LSHGEGKSKFFALGSGPGRALARKEPLFQELEYRDQAGRATLVLESDRPPPKEIAKKV
                     ADDCGVKAKNVTFIFAPTQSLAGSVQVVARVLEVALHKTHELKFPLSRVIDGVASAPL
                     APPHPDFVQAMGRTNDAIIYGGRAHLFVTGPAAEAKALAEKLPSTTSRDYGRPFAEVF
                     KTYKGDFYAIDPMLFSPACAIVTAVETGESFHAGSIDHALLDASFG"
     gene            2238..3185
                     /locus_tag="VNY06_00315"
     CDS             2238..3185
                     /locus_tag="VNY06_00315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012591387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RimK family alpha-L-glutamate ligase"
                     /protein_id="HWX13318.1"
                     /translation="MLHLDRMEASRRAFGGPKIALAVDIYDWHARDLSAAFARAGAAA
                     VPVRLSQCGFDTRRRSGLVIPGFGAGLPDAVFVRAIGSGTFESITLRLDVLHSLGTLG
                     VPVLNSARTIEICVDKAATSFALARNFIPTPCTWSVQSEQAARQIVRREGARGPLVLK
                     PLFGAQGYGLKLIRCEEDLLPLKSTEGVYYLQRFVAVERNGFRDLRLFVSEGKVIASM
                     TRHAAQWITNMKLGAQSDWLEPDAEMTDLALRAAAAVGASFAGVDLIRDASDALQVLE
                     INSMPGWRGLQKVAPFPIADCLARQVLARLGQLGAMAAG"
     gene            3182..4027
                     /locus_tag="VNY06_00320"
     CDS             3182..4027
                     /locus_tag="VNY06_00320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003608957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase"
                     /protein_id="HWX13319.1"
                     /translation="MNEIAEAFVAACRDEIEAPKPGNVHIFADGHGMTADDFLQSAEA
                     AAPALCNPAGPVGLRIFAAVEATFAAVKKNTNLGIILLCAPLAAAAQRGGEFRESLKE
                     TLKGLTREDASYAFKAILRSGPAGLGTATRHDVRDRANVTLSEAMREAAGRDKIAYQY
                     ASDFADIFGAGIKSLSDARSKWQAPWPATSAYLAFLASFPDSHLVRKNGPEAAILVQT
                     EAVEVRKRFMNTANPEERLQDLLTFDKRLKNLGLNPGTSADLTVASVFAERLTRVLIN
                     RCNNG"
     gene            4194..4703
                     /gene="fae"
                     /locus_tag="VNY06_00325"
     CDS             4194..4703
                     /gene="fae"
                     /locus_tag="VNY06_00325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018406101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016840 - carbon-nitrogen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formaldehyde-activating enzyme"
                     /protein_id="HWX13320.1"
                     /translation="MAKINKLCVGESLVGDGNEVAHIDLMIGPRGSPAETAFAHALTN
                     NKDGFTTLLAVVAPNLMCKPATILFNKVTIKNAKQAVQMFGPAQRGVAKAVADCVASG
                     VIPANEADNLFICVGVFIHWDANNDKKIQDFNYQATKQAIERAVVGDPSPQKVVAQRN
                     EVKHPFAPN"
     gene            complement(4863..>4999)
                     /locus_tag="VNY06_00330"
     CDS             complement(4863..>4999)
                     /locus_tag="VNY06_00330"
                     /inference="COORDINATES: protein motif:HMM:NF013168.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HisA/HisF-related TIM barrel protein"
                     /protein_id="HWX13321.1"
                     /translation="AAGGLRDASDLMRLREAGVSGALVASALHDGRLIGADLATAGAR
                     "
BASE COUNT         1059 a         1432 c         1455 g         1053 t
ORIGIN      
        1 tctgggcctt gggccacttg ccatcggtga tgtgaaatac aagactgaat ccggcttgtt
       61 ccagcgcatg gcgagttcat ccaaagctgt cagtttcgat tttcgcgatg cgttcgctct
      121 cgcgcgcgag attgtctgaa gcgggcacta cagttctaat cgtggcggct tcggggcggg
      181 cattggcggc agccgcgcgg cgcgcgggct accggccgct cgccttggat tttttcggcg
      241 acatcgacac ccgcgaggtt tgtgctgcga accgccgggt cgcaggcggc ctcgaaaagg
      301 gatttgcaga ggaaaaccta atccccgcgc ttaaagcact tgctaagggc gaggacgatt
      361 gcggtgtcgt ctatggtgcg ggttttgagg atcggcctgg gcttttggat tgtctcgcgc
      421 gacactggac gctcctgggt aacctatccg acgtcgtccg gcgcgtgaaa gatccggtca
      481 aactagctga gctttgtgcc ggcttgaaca ttgcgcatcc agagatcagc gcgcgtaggc
      541 ctatggagca tcatcaatgg ctcgtcaaaa gcgtgggtgg atcgggagga atgcatgtcg
      601 cgccggcgtc tgccgcgtgg gcggagggtg agggaattta ctttcaacgc aaagcagagg
      661 gtgaacccat atccatccag tttctcgccg atggacacaa tacacaggta attggattga
      721 gccgacgatg ggtggctccg gcgccaggcg aaccctttag attcggcggt gttctgcggc
      781 cggcaaatgt ttcgcctaaa atggaccggc aattgcggca aaccgcagcg gcggtcacgg
      841 cggcatgcgg gcttctcggt ctcaacacga tcgattttct cgtcaagaac aacgcttaca
      901 cactgatcga aatcaatccg cgtcccggcg cggcgctcga cattttcgag gatccgaaag
      961 gctcgctttt ccatgcccat gtcaatgcct gcatgggtcg cctgccagct tatccgctcg
     1021 aatttactgg cgcggcggcg gctgcgatcg cttatgcgcc ccgcgcgatc gcctcattgc
     1081 cagatttcaa ctggccggaa tggacagcag atcgacaaaa ggcgaagagc gcattgcgag
     1141 cgcatgatcc tttgtgcacg ataaaggcca ccgcaggaga gcccttatcg gcgcgtgcgc
     1201 tcattgagac gcggaccaat ttgcttctcg accgacttgc acatatccag aaccagaaca
     1261 gcagcaatgg gaaggaaacc accctttgag taaaagcgag ctgagtatca acacattgac
     1321 agggcaagca gtggatcgct tgatcgcgaa cgctgcccga ttgcgcctaa ccgttgccaa
     1381 aggagaggct ggcgagacgg tcattgacgc cggccagaat gcgcacggcg gcattgaagc
     1441 tggtctagca atcgccgaaa tctgcctcgg tggtctcggc accgttggcc tcgcaccgac
     1501 cggaatcaac cccaaatggc ctttcgagct ttgcgttcgc agcaccgatc ctgtcacggc
     1561 ttgcctcgct agccaatatg ccggctgggc gctttcgcat ggcgagggca agtcgaaatt
     1621 tttcgcgctc ggctcgggcc ctggccgcgc tctcgcgcgc aaggagccgc tgtttcaaga
     1681 attggagtac cgggatcagg caggccgggc tacccttgtt cttgaatccg accggccgcc
     1741 gcccaaggaa atcgcgaaaa aagttgccga tgactgtggc gtcaaggcca aaaatgtcac
     1801 attcattttt gctccgaccc aaagtctcgc gggaagcgtt caggtcgtcg cgcgcgtact
     1861 cgaggtcgcg ctgcataaga cgcatgagct caaattcccg ctctcccggg ttattgatgg
     1921 cgtggcctct gcgccactcg cgccgccaca tcctgatttt gttcaggcaa tggggcgcac
     1981 caatgacgcc atcatctacg gcggcagagc gcatcttttc gtaactggac cagcggcgga
     2041 agcaaaagcc ctagcggaaa aacttccaag cacgacctcg cgcgattacg gccggccatt
     2101 cgccgaggtt ttcaagactt ataagggcga tttctatgcg atcgacccca tgcttttcag
     2161 ccctgcctgc gcaatcgtga cggcggtcga aacaggcgag agcttccacg caggcagcat
     2221 tgaccacgcc ttgctcgatg cttcatttgg ataggatgga agcttcgcga agggccttcg
     2281 gcggtcccaa aatcgccttg gctgttgata tatacgattg gcacgcacgg gatctttcgg
     2341 ccgctttcgc tcgcgcggga gcggccgctg ttccagtccg cctatcgcaa tgcggtttcg
     2401 atacaaggcg ccgaagcggt ctcgtcattc cgggcttcgg cgccggtctg ccggacgcgg
     2461 tgtttgtgcg cgcgattggg tcgggcacat ttgaaagcat aacgctgcgc cttgatgtgc
     2521 tgcacagcct tggcaccctt ggcgttcctg ttcttaattc cgcacggacg attgaaattt
     2581 gcgttgacaa ggcggcaacg agttttgcgc tagcccgcaa ctttattcct acgccgtgca
     2641 catggtctgt ccagtccgaa caggcggcgc ggcaaattgt gcggcgcgaa ggcgcacgcg
     2701 gcccgcttgt cctaaaacct ttgttcggcg cgcaaggata cgggttgaaa ctcatccgct
     2761 gcgaagagga tttgctgcca ctcaaatcca cagaaggagt ctattatctt cagcgatttg
     2821 ttgctgtcga acgcaacggc tttcgtgatt tgaggctctt tgtctcggaa ggcaaagtca
     2881 ttgcgtcgat gacccgccat gcggcgcaat ggataaccaa tatgaaactc ggcgcacagt
     2941 cggactggct tgaaccggat gcggagatga ccgacctcgc cttgcgcgca gcggctgcag
     3001 ttggcgcctc ttttgccgga gttgatctta ttcgcgatgc gtctgacgcg cttcaggtcc
     3061 tcgagatcaa cagcatgccc ggctggcgcg gcttgcagaa ggtcgcacct tttccgatcg
     3121 ctgactgcct tgcgcggcag gtcttggcgc ggcttggcca actgggagcg atggccgccg
     3181 gatgaatgaa atcgccgagg cttttgttgc cgcatgccgg gacgaaatcg aggcaccgaa
     3241 acctggcaat gtccacattt ttgccgatgg ccacggcatg acggctgatg attttctgca
     3301 aagcgcggag gcagctgccc ccgctctgtg caatcctgca gggccggttg gcttgcgcat
     3361 atttgccgca gtggaagcca cctttgcggc ggtgaagaaa aacaccaatc tcggcataat
     3421 cctcctctgc gcgccgcttg ccgccgccgc tcaaaggggc ggcgaatttc gcgagagttt
     3481 gaaagagacg ctcaaaggac taacccgcga ggatgcgtcc tacgcattca aggccattct
     3541 tcgctcggga cctgcaggac ttgggaccgc gacccgccac gatgttcgtg accgcgccaa
     3601 tgtcacgctt tcagaagcca tgcgtgaagc ggccggccga gacaagattg cgtaccaata
     3661 tgcctctgat tttgcagata tttttggtgc gggaataaaa agtctctcgg atgccagaag
     3721 caaatggcaa gcgccttggc ctgcaaccag cgcctatctc gcgtttctcg caagctttcc
     3781 tgatagtcat cttgttcgca agaacggccc ggaagcggcc attcttgtcc aaactgaagc
     3841 ggtcgaggtg cgcaaacgtt tcatgaacac ggcgaacccg gaagagcgcc ttcaggatct
     3901 tctgaccttc gacaagcgtc ttaaaaattt gggcctaaat cccggaacaa gcgcggatct
     3961 cacagttgcg agtgtatttg ctgagagact aactcgcgtc ttgataaacc ggtgcaataa
     4021 tggttgagtg cccggagggt ggcgatagct gcctctaacc ggcccaatca gcccggtcga
     4081 atgcgagaca atgacaacac gaagcttcgt ctgcatgtgt ccggcgtctt cgacagacaa
     4141 tcggagcggg tgtcactatt tggaggaact caaccatggg ggtattcggt agtatggcaa
     4201 agataaacaa gctctgcgtc ggcgaatcgc tcgtcggcga tggcaacgaa gttgctcaca
     4261 tcgaccttat gatcgggccg cgcggtagcc cggccgaaac ggcatttgcc catgcgctca
     4321 ccaacaacaa ggatggtttt accaccctgc tcgccgtggt cgcaccaaac ctcatgtgca
     4381 agcccgcgac gatcttgttc aacaaggtga ccatcaaaaa cgctaagcag gccgttcaaa
     4441 tgttcggccc ggcgcagcgc ggcgtcgcca aggcggtggc cgattgtgtc gcaagcggcg
     4501 tcataccggc gaacgaggcc gacaatctct tcatttgcgt cggtgtgttc atccattggg
     4561 atgccaacaa cgacaagaaa attcaagatt tcaactatca ggccaccaag caagcaatcg
     4621 agcgcgcggt tgtaggcgat ccatcgccac agaaggtcgt cgcccagcgc aacgaagtga
     4681 agcatccatt tgcgccgaat taagcgccaa ttgaaaccaa ctttaagggg ctccggcccc
     4741 ttttattttg cccccaagca ctttccggct tagagcgtgt tgagccaaaa ctgggaagca
     4801 gttttgcatc cagacacgcc acaagataac aatgtggagt aagatgacga ttcgaagaaa
     4861 agtcatcttg ctccagctgt ggcgaggtcg gcaccaatca ggcggccgtc gtggagtgct
     4921 gacgcaacaa gcgccccgct tacgcctgct tccctcagcc gcatgaggtc ggaggcatcc
     4981 cgaagccccc cggcggcac
//
LOCUS       DATMPU010000017         6387 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_2800719,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000017 DATMPU010000000
VERSION     DATMPU010000017.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 6387)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6387)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6387
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2800719"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(115..1626)
                     /locus_tag="VNY06_00335"
     CDS             complement(115..1626)
                     /locus_tag="VNY06_00335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019906045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase HerA-like domain-containing protein"
                     /protein_id="HWX13322.1"
                     /translation="MVDSSVQETGRILVGKSDVYQYLTLALGNRHGLVAGATGTGKTV
                     TLQVLAEGFSRAGTSVFTADAKGDLAAISMPGDSRPHFVQRAKDIGIAYEADDFPVVF
                     WDLYGHSGHPVRASISAMGPVLLARLLELNDVQEGALNIGFKLADEQGLLLIDLKDLR
                     ALLQYIGENAAQVTKTLGNVSPATIGAIQRRLLVLENEGASGFFGEPELDINDFLRTS
                     PDGRGVVNILDAAKLMRAPRLYAIFLLWMLSELFEKLPEVGDLDKPKLVFFFDEAHLL
                     FDNAPKSLLDAVEQVVRLIRSKGVGVYFVTQNPADVPETVLGQLGNRVQHALRAFTPR
                     DQRAVKAAADTFRRNPSFDTASAITELAVGEALVSMLDSKGNPEIVARSLIAPPMAQV
                     GPISEDERQQLIRTSALADKYDTGVDRESAFEILRDRKLPVGEPQAGSSSGGLLGSLE
                     GALGKLFGPRTGSRERMSTGEIALRSAVQSAARAAGTQIGRAILRGILGGKSR"
     gene            1800..2174
                     /locus_tag="VNY06_00340"
     CDS             1800..2174
                     /locus_tag="VNY06_00340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006165185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2267 domain-containing protein"
                     /protein_id="HWX13323.1"
                     /translation="MEELITRIAAEAGIAPETAEKAVGMILGFLRKEGPPADIDALFA
                     AVPGATEAAAGGASGGAPEDPIDVSGSGLMGLAGQLTNLGLGMGQMQTIGRQVFAFAR
                     EKAGDEAVGQVVAAIPGLGQFL"
     gene            complement(2791..3372)
                     /locus_tag="VNY06_00345"
     CDS             complement(2791..3372)
                     /locus_tag="VNY06_00345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016918294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX13324.1"
                     /translation="MNTSDRLFGTFIWFLAVLAVAGLTHIIVVFALPGFAGNDTYAWI
                     SEIAKPGQLTVLPPDQPEKQLIPFADPAMAQAICPFDLSQGGLRLHADVEGDRLLALS
                     FHTPAGKLFYAMTDLAAHKGRIDIVVLTAQQLETVEAEDDEENPSQDLRLLAPMARGF
                     VLIGALAALPSERAEAEERVKSVSCGVEQIEQD"
     gene            complement(3369..3995)
                     /locus_tag="VNY06_00350"
     CDS             complement(3369..3995)
                     /locus_tag="VNY06_00350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020175477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1214 domain-containing protein"
                     /protein_id="HWX13325.1"
                     /translation="MSYGTMATKGRGFRAQIAILGKFLSAAVAGILLGLIVTWVVVER
                     SKGFGAIEAGPWTSWSRIGTPDSDPYSRAILAYFGETVLGESEGISFVADGDKRGAEF
                     NPACDYVMKGEVPASRYWTLTLFSPAGTPVPNRAGRQGFTSSEILRDGDGQFEITISR
                     HARPGNWLPSGNASRFVLVLRLYDSELNAPSGTIDAKVMPVLLKGHCE"
     gene            complement(3992..6241)
                     /locus_tag="VNY06_00355"
     CDS             complement(3992..6241)
                     /locus_tag="VNY06_00355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012590680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HWX13326.1"
                     /translation="MFDGPRSSNLRRKIARALLGADAYVDSSFHSSLEGAKEKYAAFS
                     AYMDGFHISGWRRVLNELACETLTLGIGFGVVALALAVPAFQETSDDWLKKTDFAVTF
                     LDSYGLEVGSRGIKHDDKVSFDDLPQNLVHAVLATEDRRFFEHFGIDVIGTFRALTVN
                     AKADTVVQGGSSITQQLAKNLFLSNQRTLERKIKEAYLAVWLEFHIPKREILRLYLDR
                     AYLGGGAFGVQAASEFYFGKSVRDLSLAEAAMIAGLFKAPTRFAPHINLPAARARAAD
                     VLNNMVEAGYLNEGQIYPALRNPATPVDRSRDITPEWYLDYAFAEVKKLAEEKKLGDE
                     RVLTVRTALDTNLQRHAEQTLEDQLRLHGPQFKAKQGAMVIGEPLGALKAMVGGRDYG
                     ASQFNRATDALRQPGSSFKPFVYLTALETGKFKPSTIVVDGPICIGNWCPRNYKGSFK
                     GRVPLVVALAQSLNTVAVRLSVEIGRANPVPGHNNTWDEAKRGRAKIIETARLMGLTT
                     PLVDTVSLPIGAAEVTVIDMASAYSTFANGGLRAPPYAAVEILNSRGEVIYRHDRDAP
                     PLRQIVDHDVILTMVSMMTQVVEAGTGRRAHLDNTEVAGKTGTTNGYTNAWFIGYTGN
                     YVGAVWYGNDDSTPMEKMTGGTLPAATWHDVMQYAHQGIELKPLPGRPAVAASHAPVA
                     AAAAEAPRRPPSLSAATATVLMAIEAAMKASQTKSMDAAIAVEDLPPRGAMPFRPAGA
                     VNAPAGGGG"
BASE COUNT         1407 a         1823 c         1833 g         1324 t
ORIGIN      
        1 tgagtcagcg ctcaatgccg ccggtataac acaaatccgt cacaggctgc atggcttacc
       61 ctcaagactg cagggcactt gtggctgaag cgcaatcgtc ctgacgctac gcgcctacct
      121 agacttgccg cccaatatcc cgcgcaatat ggcgcgccca atctgggtcc cggctgcgcg
      181 ggctgctgac tgcactgccg atcggagcgc gatttcaccc gtcgacatgc gctcacgact
      241 tcctgtcctt ggtccaaaaa gcttacccaa cgcaccttcg agcgagccaa gcaaaccacc
      301 agacgaacta ccggcctgtg gctcgccaac cggcaacttc cggtcacgca aaatctcaaa
      361 ggcggattcc cgatcgactc cagtatcgta tttatccgca agcgcgcttg ttcggataag
      421 ctgctggcgc tcgtcctcgg agattggacc gacctgtgcc atcggcggcg caatgaggct
      481 tcgggcaacg atttcaggat tccctttgga atcgagcatg gagaccaggg cctcgccaac
      541 cgcaagctcc gtgatcgcgc ttgcggtatc gaaggatggg ttgcggcgaa aagtatccgc
      601 tgccgccttg acggccctct ggtcgcgcgg agtaaaggcc cgcagggcat gttgaacccg
      661 attgccgagc tggcctagaa cggtttccgg aacgtcagcc ggattttgcg tgacgaaata
      721 gacgcccacg cctttcgaac gaatgagccg gacgacctgc tcgaccgcat ccaataagga
      781 cttcggcgca ttgtcgaaga gcaaatgcgc ttcatcgaaa aagaaaacca gctttggctt
      841 gtcgagatcc ccaacctcgg gcagcttttc gaacaattcg gagagcatcc acagcaggaa
      901 aatcgcgtaa aggcgcggtg cgcgcatcag ttttgcggca tctaaaatgt tgacgacgcc
      961 gcgcccatcg ggactggtgc gcagaaaatc attgatgtcg agttccggct cgccaaaaaa
     1021 gcccgatgca ccttcatttt cgaggaccag cagccgacgc tggatcgctc ctatcgtcgc
     1081 tggcgacaca ttccctaagg tcttggtcac ttgtgcggcg ttctctccaa tatattgcaa
     1141 gagcgcccgg agatccttga gatcaatgag caaaagccct tgctcatcgg cgagtttaaa
     1201 gccgatgttg agcgctccct cctgaacgtc gttgagttcc agtaaccttg cgagcaacac
     1261 gggccccata gccgaaatgc tagcacgaac tgggtggccg ctgtgaccat agagatccca
     1321 aaacacgaca ggaaagtcgt cggcctcata ggcaatcccg atatcttttg ctcgctgcac
     1381 aaaatgcggc ctagagtctc ctggcatgga gatggctgcg agatctcctt tggcatcggc
     1441 agtaaaaacc gaggttcctg cgcgtgaaaa tccttcagcg agaacttgca aggtcacggt
     1501 cttgccggtg ccggttgctc ctgcaacaag tccgtgccgg tttcccagcg ccagggtgag
     1561 gtattgataa acgtcgctct tgccgaccag aatgcggcca gtttcttgaa cagagctgtc
     1621 gaccatggat ctcctcgaaa agtggtggga gacataaatt gctgtgttgc tcacctggcc
     1681 gctacaagac ggagtgtagc catagcgccc gtggatcctg aacggctcgc atgtacctta
     1741 attttatccc aaacagagtg gcaagtgcga gtaatttcta cgtgccaggg aggagtgaga
     1801 tggaagagtt gatcactcgc attgctgcag aggctggtat tgctccggaa accgcagaaa
     1861 aagcggtggg aatgattctt ggcttccttc gcaaggaggg gccaccagcg gatatcgatg
     1921 ctttattcgc ggccgtacca ggcgctacgg aagcggctgc cggaggggcc agcggcggcg
     1981 cgccggaaga cccgatagat gtgagcggca gcggactcat gggccttgct ggccagctga
     2041 ccaatcttgg tctcggcatg gggcagatgc agacaatcgg acggcaagtt ttcgccttcg
     2101 cgcgtgagaa agcaggcgat gaggctgtcg gccaagtcgt cgcggcgatc cccggccttg
     2161 gccagtttct ttagaggcta agagtgctga ggcactgctt gataggaact cttgtacgcg
     2221 tcttacacaa ggcttcggcg cccaatgttg cctgctcaga gtgcacaacg gacagagttg
     2281 gtagctaaag ttggaagaac cacctgcttg acggggttca tcagctccgg gtaagcctgc
     2341 gggtgaaatt agtttccaac gtattaaaac cgctttggcc atattcgatc atccggagaa
     2401 actttatcaa gggcggcacc ttaggctgtt agaggcgaag aatcacgctg cccgcgggga
     2461 tggtgcaaaa ctatttggag aaaaagcaaa tgggcgaact cattgctttc aagccagcca
     2521 gacgctgcgt gcccttcagg gcggcgcctg ttgacgctgg cacggtagtg attttcaccg
     2581 gggttcggca agaacgctta gaaagcttcg aaagcaatac agtaaaggct agcgaggctc
     2641 agcgcgcacc aagggttccg gtgcggcgcg caagaaaatg caaaggaaag gtcggcgcca
     2701 gccctaaggt gtaaggactt caacgcgctg cgttaagctg agagaagtaa cgccctccac
     2761 cgtaaaaaac ggatataggg cggtaggttt tcaatcctgc tcgatttgct cgaccccgca
     2821 ggacaccgat ttgacccgtt cctccgcttc tgccctttcg cttggcaagg cggcgagagc
     2881 accgatcaat acaaatcctc gggccattgg cgcaaggagt cgcaagtcct gagaagggtt
     2941 ttcttcgtca tcttcggctt cgacagtctc cagctgctgt gcggtcaaaa cgacgatgtc
     3001 aattctgccc ttgtgcgccg ccaggtctgt catcgcatag aagagcttcc cggctggtgt
     3061 gtgaaacgaa agcgcaagaa gccggtcacc ttcgacatcg gcatgtagcc gcaaaccgcc
     3121 ttgcgatagg tcgaaggggc aaatggcttg tgccatagcc ggatcggcaa atggaatgag
     3181 ctgcttttcg ggctggtcgg gtggcagaac ggtcagctgt cctggctttg ctatctccga
     3241 aatccaggca taggtgtcgt ttcctgcaaa tcccggtagc gcgaaaacga cgatgatgtg
     3301 cgtcagtccg gcgacggcca gcacagctaa gaaccagata aacgtcccga aaagccgatc
     3361 ggacgtgttc attcgcagtg acccttcaga agtactggca tcaccttggc atcgatggtt
     3421 ccagaagggg cgttgagttc gctatcataa agccgcagaa ccaggacaaa tctcgaggcg
     3481 ttgccggaag gtagccaatt gcccggcctc gcgtggcggg atatcgtgat ttcaaattgg
     3541 ccgtcgccat cgcgaagaat ttcgctcgac gtgaagccct gcctcccagc tcggttagga
     3601 accggtgtgc ccgctggaga aaaaagtgtt agagtccagt agcgcgaagc cggtacttcg
     3661 cccttcatga cataatcgca agccggattg aattccgcgc cacgtttatc gccgtcggca
     3721 acgaagctta tcccttccga ctcgcccaaa actgtttcac caaaataggc gagaatcgcg
     3781 cggctataag gatcggagtc cggcgtccca atgcgagacc agcttgtcca cggtcccgcc
     3841 tcaatggccc cgaagccctt actgcgctcg acgacaaccc atgtgacgat caaaccgaga
     3901 agaattccag cgacggcagc ggacagaaat tttcctaaaa ttgcgatttg cgcccggaac
     3961 ccgcgaccct tcgtagccat cgtgccgtag ctcatccgcc tccccctgcc ggagcgttta
     4021 ccgcccccgc gggcctgaat ggcattgctc cacgaggtgg aagatcctcg accgcaatcg
     4081 ctgcgtccat ggatttcgtt tgcgaagcct tcattgcggc ttctatagcc atcaggactg
     4141 ttgccgtggc cgctgacaga cttggcggcc gacgtggtgc ttcggcggcg gcagccgcaa
     4201 cgggcgcgtg cgacgcggct acagctggcc tgcccggcag cggcttcagc tcaatccctt
     4261 gatgcgcata ttgcataaca tcgtgccagg tcgcggcggg caatgtgccg cccgtcatct
     4321 tttccattgg cgtgctatca tcattgccat accagacggc gccaacgtaa ttgccggtgt
     4381 agccgatgaa ccaggcattc gtgtaaccgt ttgtggtccc ggtcttaccg gcaacctcgg
     4441 tgttgtcgag atgggctcgt ctgcccgtgc cggcctcaac cacctgcgtc atcatcgaaa
     4501 ccattgtcaa aatgacgtcg tgatcgacaa tttgtcgcag cggcggcgcg tcgcggtcgt
     4561 ggcggtagat cacttcgccg cggctgttca aaatctcgac agccgcataa gggggcgcgc
     4621 gcaatccgcc attcgcgaat gtgctatagg ccgaagccat atcgataact gtgacctcgg
     4681 cagcgcctat gggcagagag acggtatcga cgagcggagt cgtcagaccc atcagccggg
     4741 cggtttcgat aatcttggcg cggccgcgct tggcctcatc ccaggtgttg ttgtgccccg
     4801 gcacaggatt ggctctgcca atctcgacag acaagcgaac ggcgaccgtg ttcagcgatt
     4861 gtgccaaggc aacgacgagg ggtacgcggc ccttgaaact tcctttgtaa ttacgcgggc
     4921 accagtttcc gatacaaatc ggcccgtcaa cgacgatggt ggaaggcttg aactttccgg
     4981 tctcaagcgc cgtcagataa acaaagggtt tgaacgatga gcctggctgg cgcagcgcgt
     5041 cggtcgcgcg attgaattgg cttgcgccat agtcccggcc gccaaccatt gccttgagcg
     5101 cgccaagcgg ctcgccaatc accatcgcgc cctgtttggc cttgaactgc ggcccgtgca
     5161 ggcgcaattg atcctcgagg gtctgctcgg cgtgtctttg cagattcgtg tcgagagcgg
     5221 tgcggacggt gagcacgcgc tcgtctccaa gtttcttttc ctcggcaagt ttcttcacct
     5281 cagcaaaggc gtaatcgagg taccattccg gggtgatatc ccggctgcgg tcgaccggcg
     5341 tcgccggatt gcgcaaggcc ggataaatct gtccctcatt gagatatcct gcctccacca
     5401 tattgtttaa aacgtctgct gcccgcgcac gcgcggccgg cagattgata tgaggggcaa
     5461 accttgtcgg cgccttgaag aggccggcta tcatcgcggc ttcggcaagc gagaggtcac
     5521 ggaccgactt gccaaaatag aattccgacg ccgcctggac accgaaagcg ccgccgccaa
     5581 gataggctcg gtcgagataa agccgcagta tttcgcgttt gggaatgtgg aactcgagcc
     5641 aaaccgcgag ataggcttcc ttgattttgc gctcaagcgt cctttggttt gaaagaaaaa
     5701 gattctttgc aagctgctgg gtgattgagg agccgccttg gaccacggtg tcggccttgg
     5761 catttacggt caaggcacgg aatgtcccta taacgtcgat accaaaatgc tcgaagaagc
     5821 gccggtcctc ggttgcaagc acggcatgaa ccagattctg cggcaaatcg tcaaaagaga
     5881 ccttatcgtc gtgcttgatt ccgcgactcc cgacctccag accgtagcta tccaagaacg
     5941 tcactgcaaa atcggttttt tttaaccagt cgtccgaggt ttcctgaaag gcggggaccg
     6001 caagcgcgag cgcgacaacg ccaaagccaa ttccaagggt aagcgtttcg caggcgagtt
     6061 cgttgagaac ccgccgccag ccagagatat ggaatccatc catataggct gagaaggcgg
     6121 cgtatttttc ctttgcgcct tccaagctcg aatggaacga ggaatcgacg tatgcatcgg
     6181 cgccgaggag agcccgggca attttccggc gaagattgga cgatcgcggc ccgtcgaaca
     6241 aagtggaacg ctcccttgca tgccgccgcg aactgtgagg aaagcggcgt gagtttaaca
     6301 gtcttcttga cgtctgccaa agccgcgtgt caactctggc cgcatcgccg cataaaaagg
     6361 acgtgatcga tgccgcagcc gtcgccc
//
LOCUS       DATMPU010000018         5139 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_3424225,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000018 DATMPU010000000
VERSION     DATMPU010000018.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 5139)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5139)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5139
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3424225"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..413)
                     /locus_tag="VNY06_00360"
     CDS             complement(<1..413)
                     /locus_tag="VNY06_00360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015929711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX13327.1"
                     /translation="MMNDATQSARVELPRKTAVRFIVLLGIVSLFADMTYEGARSVTG
                     PYLGMLGASAAVVGLVAGFGEFLGYALRLVSGYLSDRTGSYWLITIIGYGVNLLAVPL
                     LALTGRWELAVLLIVAERAGKAIRTPARDAMLSHAG"
     gene            complement(628..1047)
                     /locus_tag="VNY06_00365"
     CDS             complement(628..1047)
                     /locus_tag="VNY06_00365"
                     /inference="COORDINATES: protein motif:HMM:NF015181.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein MobB"
                     /protein_id="HWX13328.1"
                     /translation="MRVIRLAGWSGAGKSTLIVKLIPYLRENGLSASTLKHTHHAFDV
                     DQPSKDSYLHREAGACPALRAHARAARSGRASPCGAVRLHGASRSHSGYKRDTHAKIE
                     VHRLGNTKPFVYPEDATFAALATDATEGLPSHLPCVS"
     gene            1128..1368
                     /locus_tag="VNY06_00370"
                     /pseudo
     CDS             1128..1368
                     /locus_tag="VNY06_00370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012335651.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
     gene            1365..2342
                     /gene="dgcA"
                     /locus_tag="VNY06_00375"
     CDS             1365..2342
                     /gene="dgcA"
                     /locus_tag="VNY06_00375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018264480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016855 - racemase and epimerase activity,
                     acting on amino acids and derivatives [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-D-Glu racemase DgcA"
                     /protein_id="HWX13329.1"
                     /translation="MKVTATIERFPILGSFVISRGAKTEAAVVVVAIEDEGFGGRGEA
                     VPYARYGEGLEGTLAQIATVRSAIEAGVDRATLQTLLPPGAARNAIDCALWDLEAKRS
                     GTPVHVLAGMPTPVPVETAFTISVGTPEEMARAAAKATAQPLLKIKLAGEGDAARLAA
                     VRSAAPDARLIVDANEAWAESDLEHNLEACAKSGVLLVEQPLPAKQDAMLANVPHIVP
                     ICADESVHDRTGLEALRARYDAINVKLDKTGGLTEALTVAKQAETLGFELMIGCMVAT
                     SLSMAPALLLASRARFVDLDGPLLLARDRPDGIRYHGSVIHPPTRELWG"
     gene            complement(2356..3582)
                     /locus_tag="VNY06_00380"
     CDS             complement(2356..3582)
                     /locus_tag="VNY06_00380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020176804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWX13330.1"
                     /translation="MNADTSPNAGYPRPPYSQSRALMAFYLSIVALTLFLPIGLHLPY
                     FPVWLAARGFSDSEIAVALGLPTLLRVAAAPVVTAIADKRGIAATLAACAAAAFAAYC
                     FLAIVKGFLTIFAGAIFAMIALGLMVPLADALTLAGIRRVEVMGLGRVAYARIRVWTS
                     IGVLGTMLASGRIVSLFPGERIIFALAGLTLLPALVTIFAATKLKHLHTRGTHKGGLT
                     TDPTQLRLAFAFIGAAALIQSSHAEYYSFATLHWKAISLAPNFIGVAWAIGVASETVL
                     FLLAARYFAAEKNAATFLVFGALGALFRWLAMSTDPGPLLLIVLQAIHGLSFGATYFG
                     SVLLLGGIARETHRARMQGWLASASALSLALATFACGWLTSHFGEKAYLAMAALAVAG
                     LMLALIAGAIKRRIRT"
     gene            3830..5092
                     /locus_tag="VNY06_00385"
     CDS             3830..5092
                     /locus_tag="VNY06_00385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012589501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HWX13331.1"
                     /translation="MEQAQPVSPATVVIIGGGQAGGEAATLLRQNRFEGRILLVGAER
                     CLPYMRPPLSKAFLAREIEKEALIYKAAAAYEKARVEMRFGVTVEEIDRRAKNLKLSS
                     GATLGYDKLVIATGGRARPLPVPGADLRNIFYLRTIADVEALQPQMAAGRHLVIVGGG
                     YIGLEAAAVAVKRGLAVTVVESAPRVLARVTAPDLSNFYERIHRKAGVEIRTGVTVTG
                     FLVGGEEGTVGKVECAGGESFAADFVLIGIGLVPNSELAEQAGLDVDDGIIVDAASRS
                     SDPDIYAIGDCAMHAHHGFLRRKIRLESVPNALEQARAAAASLTGRPIPEAPPPWFWS
                     DQYDLKLQMVGLSDGYDELAIRGSMDSSAFIAFYLKEGVVVAADSVNRAGDFMAAKRI
                     IGERMKISTVRLADETASLKSLLQSVAA"
BASE COUNT         1111 a         1501 c         1526 g         1001 t
ORIGIN      
        1 cccgcatgcg acagcatcgc atcgcgcgcg ggggtgcgga tcgccttgcc cgcccgctcg
       61 gccacgatca agagaaccgc aagctcccag cggccggtca acgcaaggag cggcacggcc
      121 aagagattga cgccatagcc gatgatggta attagccaat agctgccggt gcgatcgctg
      181 agatagccag aaacaagccg caacgcgtag cccagaaact cgccaaaacc cgcgaccaaa
      241 ccgacgacgg cggcgcttgc tccgagcatg ccaaggtaag ggccggtgac gctgcgcgcg
      301 ccttcatagg tcatgtccgc gaacaggctg acgatgccaa gcagcacgat gaacctcacc
      361 gcggttttcc tcggaagctc gacgcgcgcg gattgcgtgg catcattcat catcgtgggt
      421 atccctttgt gaaatgggac atggagcaaa gcgtgacgac aaaaggttcg gcaatttttc
      481 tattcgccaa tcagctctct gctctcgcgc atatcttgcg ctcgccgatc ggataagctc
      541 agcaccgtca ctctttgtcg aggcgccagc gcttcatcga gaggcgaggc gtgtgcgagt
      601 atgagggtcg cgacggccgc tatatcgtca agatacgcac ggcaaatgtg acggtagccc
      661 ctccgtcgcg tcggtcgcta gagccgcaaa cgtcgcgtct tcgggataaa caaatggctt
      721 tgtattccct aagcggtgga cttcaatctt tgcatgggta tcacgcttat aaccagaatg
      781 agatcgactg gcgccatgca gcctaacagc accgcaaggc gaggctcggc cgcttcgcgc
      841 agctcgtgca tgagcgcgaa gcgccgggca agcaccagcc tcacgatgca aataggaatc
      901 cttactcggc tgatcgacat cgaacgcatg atgcgtgtgc ttgagagtcg acgcggaaag
      961 gccattttct cgcaaatagg ggatcaactt tacgatcaag gtcgacttgc ccgcgccgct
     1021 ccaaccggca aggcggatca cgcgcatagt tcaaaactcc accaaggttt gctggaaatc
     1081 caatcccgca tcccgtagct tcttcaaagt caaaatctcc ggcaaacatg ctatttgggg
     1141 ttttctccat gctccttgac ttgcgtggct tgcaatgtcc attgccgccc tgcggacacg
     1201 taaaatgctg cgaaagctgg cgtatggcga ctgccttgtc gttgaatgct ccgacccgct
     1261 cagcgtcatc gacattccgc acctcctgcg cgagacaggg ataggctact ccgccacgag
     1321 gcagcaaacg gtttatttat attccacatc gagcgcggaa aagcatgaaa gtcacagcca
     1381 ccatcgaacg cttcccgatt ttggggagct ttgtcatctc tcgaggcgcc aagacggaag
     1441 cggccgtggt tgtcgtggcg attgaggatg aaggtttcgg cgggcgcgga gaagccgtcc
     1501 cttatgcacg ttatggcgaa ggcctggaag ggacgcttgc ccaaattgca acggtacgct
     1561 ccgcaatcga ggctggcgtg gatcgcgcga ccctgcaaac tcttctccct cctggcgccg
     1621 cgcgtaatgc tatcgactgc gcgctctggg atttggaggc taagcgaagc ggtacgccgg
     1681 tccatgtcct cgccggcatg ccgacgcctg tgcctgtcga gactgccttc acgatttcgg
     1741 tcggcacacc ggaagaaatg gcgagggccg ccgccaaggc gactgcgcag ccgcttctca
     1801 aaatcaaact cgcgggtgaa ggcgacgcgg cgcggctcgc ggccgtgcgc tccgccgcgc
     1861 ccgatgcacg cctcatcgtc gacgccaatg aggcgtgggc agaatctgat ctcgaacaca
     1921 atctggaggc ttgcgccaag tctggcgtgc tattggtcga gcagccgctt ccggccaagc
     1981 aggacgctat gctcgcaaac gtcccgcata ttgtgccaat ttgcgccgat gaaagcgtgc
     2041 atgaccgtac cgggctcgaa gccttgcgcg cgcgttacga cgcaatcaat gtcaagctcg
     2101 acaagaccgg agggctgacc gaggctttga ctgttgcgaa gcaagccgaa acgctcggct
     2161 ttgagctcat gatcggctgc atggtcgcga cctccctttc catggcgccc gcgcttttgc
     2221 tcgcgtcccg cgcgcgtttc gtcgatctcg acggcccctt gttgcttgcg cgcgatcggc
     2281 cggatggaat tcgttaccat ggctcggtca tccacccgcc aacgcgtgag ctttggggct
     2341 gatgcttatc gtggttcatg tccggatccg ccgtttgata gctcccgcga tgagcgcgag
     2401 catgagacct gccacggcga gagcagccat ggcgagatag gccttttccc caaaatggct
     2461 tgtcagccag ccacacgcga aggtcgccag cgcaagactc aatgccgagg cggacgcgag
     2521 ccagccctgc atccgtgctc ggtgcgtttc ccgcgcaatg ccgccgagca agagcacgct
     2581 tccgaagtag gttgcgccga aacttagacc atgtattgcc tgcaggacaa tgaggagcaa
     2641 gggtccgggg tccgtgctca tggcgagcca acggaacaga gcgccgagag cgccaaaaac
     2701 aaggaaagtg gcggcatttt tttcggcggc gaaatagcgc gcggccaaaa ggaatagcac
     2761 ggtttccgaa gcaacgccga ttgcccaggc aacgccgata aagttcggcg ccagacttat
     2821 ggccttccag tgcaatgtgg caaaggagta atactctgcg tggctcgact ggatcaatgc
     2881 cgccgcgccg atgaaggcga atgccaggcg cagctgcgtc gggtcggtag tcaaaccacc
     2941 cttgtgcgtg ccacgcgtgt gcagatgttt cagcttcgtg gcagcgaaaa tcgtaacaag
     3001 tgcaggcaag agcgtcagcc ctgccagcgc aaaaatgatc ctctcacccg gaaataagct
     3061 gacgatccga ccggatgcaa gcatcgttcc caagacgcca atcgacgtcc atacacggat
     3121 gcgggcatag gcgacacggc caaggcccat aacttccacg cggcgaattc cggcgagggt
     3181 aagtgcgtcg gcgagcggca ccatcaagcc cagcgcgatc atggcaaaaa tcgcgccggc
     3241 aaaaatggtt aagaaccctt tgacaatagc aaggaagcaa taggccgcaa atgcggcggc
     3301 ggcgcatgct gcaagggttg ccgcgatgcc gcgcttgtcg gctatcgccg tgacaacagg
     3361 tgctgccgcg acacgcaaca acgttggcag gccgagcgca accgcgatct cgctgtccga
     3421 aaatccccgc gcggcgagcc acactgggaa atagggcaga tgcagcccga ttggcaaaaa
     3481 cagtgttagc gcgacgatcg agagatagaa ggccattagc gcgcgggact ggctgtacgg
     3541 cggtcgcgga tagcctgcgt taggactggt gtccgcgttc attgcaacgg actcgcgcgc
     3601 agatgcgcca acttgccgcg ctcccctcgt cacaagtatc tctttaagag aaacaatttt
     3661 caccgtgctg cctatctaga aaaaatgcgc attgctaaac ggtaagaacc tgatcattga
     3721 aatagcctag gctcaggcgt aatgcgccca tagcgccggg agcaatcaca ggaatttgtc
     3781 gaagtcaaaa gatctatccc tctttcgcgc aaaggtggcg gatttggtaa tggaacaagc
     3841 tcagccagtt agccctgcaa cggtcgttat cattggcggc ggacaggcgg gcggcgaggc
     3901 cgcgaccttg ctgcggcaaa accgtttcga gggccgcatc ctacttgtcg gcgcagaacg
     3961 atgccttccc tatatgcgtc cacccttgtc gaaagcattc cttgcacgcg aaattgaaaa
     4021 ggaggcgctg atctataagg ctgcggcagc ctatgagaaa gctcgtgtgg aaatgcggtt
     4081 cggggtcacg gtcgaagaga tcgaccggcg ggctaaaaac cttaagctga gtagcggtgc
     4141 aacgcttggc tacgataaac ttgtgatcgc aacgggcggc cgcgcgcgcc cattgcccgt
     4201 gccgggagct gatctacgca acatttttta tttgcgtaca attgccgatg tcgaggcatt
     4261 gcagccgcag atggcggccg gccggcacct tgtgattgtc ggcggcggct atattgggct
     4321 cgaagccgcc gctgttgcag tcaagcgcgg gctagcggtc accgtggtcg aaagcgcccc
     4381 gcgggtgctc gcgcgcgtca ctgcgcccga cctttcaaac ttctatgaac gcatccatcg
     4441 caaagctgga gttgaaatcc gcaccggcgt tacagtcacc ggttttttgg ttggaggcga
     4501 ggaaggcacc gtgggaaaag tcgaatgcgc tggtggcgaa tccttcgctg ccgattttgt
     4561 gctgattggc ataggtcttg tgcccaattc ggaactggca gaacaagccg ggctcgacgt
     4621 ggatgacggg atcatcgtcg atgcggcaag ccgcagcagt gatcctgaca tctatgcgat
     4681 cggcgattgc gcaatgcacg cgcatcatgg ttttcttcgc aggaagatac gcctggaatc
     4741 tgttcccaat gcgctcgaac aggcgcgcgc ggcagccgcg tcgctaaccg ggcggccaat
     4801 tcccgaagct ccgccgccct ggttctggtc cgatcaatat gacctcaagc tgcagatggt
     4861 cggcctctcg gacggctatg atgagctcgc gatccgcggc agcatggatt cgtccgcttt
     4921 cattgcattt tatctcaagg aaggggttgt cgttgctgcc gactcggtca atcgggcggg
     4981 cgatttcatg gctgccaaac ggatcattgg cgagcggatg aagatttcca cggtgcggct
     5041 tgccgatgaa acggcgtcgc tcaaatcgtt gctccagtct gtggccgcgt agcaccagtt
     5101 ttgtcccatt tcgccctcaa atcgtaccaa aatgtttca
//
LOCUS       DATMPU010000019         2874 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_2187425,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000019 DATMPU010000000
VERSION     DATMPU010000019.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 2874)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2874)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2874
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2187425"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(18..566)
                     /locus_tag="VNY06_00390"
     CDS             complement(18..566)
                     /locus_tag="VNY06_00390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018261151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 2-dehydrogenase subunit 3 family
                     protein"
                     /protein_id="HWX13332.1"
                     /translation="MREVDRRSKYNRRLFLQGAATAVPTVAIATSAGLGLSDAWADDA
                     TALTPATLKTLVKVARDIYPHDFLGDSYYVTAIKPWDGKAAKDPSVKSLINDGVARLD
                     QEANDRHKVPYAQVQWEADRVALLQRIEQTAFFQQIRGDLVVSLYNQKELWPKFGYEG
                     SSAEHGGYIKRGFADIDWLPKV"
     gene            complement(599..994)
                     /locus_tag="VNY06_00395"
     CDS             complement(599..994)
                     /locus_tag="VNY06_00395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006610406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HWX13333.1"
                     /translation="MAKAVHSMIRVFDEAKSLDFYARALGLEVADHLEFPDFALIYLR
                     HPSSPFEVELTVNRDRNEPYSLGDGYGHLAVVVDDVDAEHSRFERENLSPGPLRDFKH
                     DGRTLARFFFVADPDGYKIEVIQRGGRFG"
     gene            complement(995..1315)
                     /locus_tag="VNY06_00400"
     CDS             complement(995..1315)
                     /locus_tag="VNY06_00400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016844455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HWX13334.1"
                     /translation="MALPAAKATDGPTLFRQQCATCHTTKPSEPVRQGPSLFRIVGRP
                     AGKVDGFHYSAGFAKADFVWDEAMLDAWLANPQAIIPGANMAYRQPKTETRAAIIAYL
                     KELN"
     gene            complement(1961..2197)
                     /locus_tag="VNY06_00405"
     CDS             complement(1961..2197)
                     /locus_tag="VNY06_00405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015688237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX13335.1"
                     /translation="MKKPLTIPDEPQPRNIARADLVMTEGFGLQVDGRMKTVFPTLAA
                     AQKRARDLKAEFPMLQVKVYDAAEKKLLELSPTE"
     gene            2521..>2874
                     /locus_tag="VNY06_00410"
     CDS             2521..>2874
                     /locus_tag="VNY06_00410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module antitoxin"
                     /protein_id="HWX13336.1"
                     /translation="MIAMAIAWNGHTRSMICDLIARLHELDENSEARVWNLVETFARH
                     GTDEDKAELREKIRVTVMSRRGVMLSKRGSKSPAGLTAAAKAAYAALEPSDLINKHAW
                     LFRDGWVDESADEIED"
BASE COUNT          569 a          805 c          837 g          663 t
ORIGIN      
        1 ccgtttgttg cccgggccta gaccttcggg agccagtcga tatcggcgaa gccgcgtttg
       61 atgtagccgc catgttcggc ggaggagccc tcgtagccga acttgggcca tagctccttc
      121 tggttgtaga gagagacgac gaggtcgccg cgtatctgct ggaagaaggc ggtctgctcg
      181 atgcgctgca acagcgccac gcgatcggcc tcccattgca cctgcgcata gggaactttg
      241 tgacggtcgt tggcctcctg atcgagcctg gcgacgccgt cattaatcag cgattttacg
      301 gacgggtcct ttgccgcttt gccatcccat ggcttaattg cggtcacgta gtagctgtcg
      361 cccaaaaagt cgtgcggata gatatcgcgc gcgactttca ccagcgtctt cagtgtagcc
      421 ggcgttagcg cggtcgcgtc gtcggcccag gcgtcgctaa gccccaagcc cgcgcttgtt
      481 gcgatcgcaa cggtcggcac cgctgtggcc gcgccttgga gaaagagccg gcgattatac
      541 ttgcttcgac gatcgacttc tctcatgggt atcctccatt ggcgtttgtt gactgcgatt
      601 aaccgaagcg gccaccccgc tggatgactt cgattttgta accgtcgggg tcggcgacaa
      661 agaagaagcg cgccaacgtc ctgccatcat gcttgaagtc gcgcagcggc ccgggcgaga
      721 ggttttcgcg ttcgaatctt gagtgttcgg cgtcgacatc atcgacgact acggcaaggt
      781 ggccgtagcc gtcgccaagc gaatagggct cgttgcgatc acgattgaca gtcagttcga
      841 cctcgaaggg cgaggaagga tgacgcagat agatcagcgc gaaatccgga aactcgagat
      901 gatcggcaac ttcaagaccc aaggcgcgcg cgtagaaatc gagcgatttc gcctcgtcga
      961 acacgcggat cattgagtga acggcctttg ccattcagtt cagctccttg agataggcga
     1021 tgatggcggc gcgggtctcc gtcttaggct ggcggtaagc catgttggcg ccgggaatga
     1081 ttgcctgcgg gtttgccagc caggcgtcga gcatggcctc gtcccagacg aagtcggcct
     1141 tcgcgaaacc ggccgagtag tggaagccgt ccaccttgcc ggcaggcctg ccgacgatcc
     1201 tgaacagcga gggaccttgc cgaaccggct cggacggttt cgttgtgtgg catgtcgcgc
     1261 attgctgtct gaatagcgtc ggaccatcgg tcgccttggc ggcgggcagc gccatctgcg
     1321 cggcggcgca aaaccccagc ccgattgccc atgttccgct cagctttgtc atacgcatca
     1381 ccgttggcgt gcagacttta ggtcgcggca aacaagctgt ggtagtagcg ggctatttac
     1441 ggcagaacaa aaagggtcgt tctttgggcc agaccggtcg tcggcgggga taggcgccac
     1501 atcgcgggac ggatgtctgc ttaaaagcgg acgtattgct cctggtttat gatgtccgct
     1561 cctcgcccct tagcgcagat ttatcagtcc ctttgtcacg tgcgacctta aggcaaccat
     1621 caagtgggtt tttcctttta aaaaaactga aaatcttgcg tgcgaaccag tcgcaccggt
     1681 ccgcatcctc atcccgccag ggatttgacc acccccaccc agtttagcgg aataaatccg
     1741 cagagatgga gagagatgcc acgcgctcct ggttaccaga gaatgtgtcc ggcccgctct
     1801 tgtcttcccg tgcctggcgt taaccgatgg tcagagtgtt tgcgacgcgt ttgaactgct
     1861 ttcctatctg cgccgaaaaa gccgatgatg ccgcgcttag ccgacggcat tcgtttttcg
     1921 ctagtcggac aatctggtca attttgatgg tctcatagcg tcactcagtc ggcgacaatt
     1981 ccagcaactt cttttccgca gcgtcgtaca ccttgacttg gagcatcgga aactcggctt
     2041 tgagatccct cgcccttttc tgtgccgctg caagcgttgg aaagaccgtc ttcatccggc
     2101 catcgacctg caagccaaag ccctcggtca tcactaggtc agcgcgtgcg atgttgcggg
     2161 gttgcggctc gtccggtatt gtcagaggct ttttcatggc aactccttgg gttgccccct
     2221 ccctatctaa ttactgccct cctcgataga agagcaacgc ctttcgcgac gctttcctgg
     2281 tcgttcaatt ttcgactttg tagcggtcaa taacggccat caggcctcgt caatgcagag
     2341 tctatttttt ccccgacagt ggaccttggt ttagtgactg tgaggacccc aattgttacg
     2401 cggatcgaat gttatggaat atgccagctg tacagtcata agccgcgatg gcgtcccgat
     2461 ggttatggtt ttggcgaacc aatcccgaca tggggaccga tcatcgcgtt tagggacgaa
     2521 atgattgcga tggctatcgc ctggaatggc catacgcgct cgatgatttg cgatcttatc
     2581 gctcgccttc atgaacttga cgaaaattcg gaggcgaggg tatggaacct cgtagaaaca
     2641 tttgcgaggc acggaaccga tgaagacaag gctgagctgc gtgagaaaat ccgtgtgacc
     2701 gttatgtccc gtcgcggcgt aatgctttcc aagcgcgggt cgaagagtcc tgctggactg
     2761 actgcggcag cgaaggccgc ttacgccgca ctcgaacctt cagacctcat caacaagcac
     2821 gcgtggttgt tccgcgacgg atgggttgat gagtctgctg atgaaatcga agat
//
LOCUS       DATMPU010000020         7562 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_1565065,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000020 DATMPU010000000
VERSION     DATMPU010000020.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 7562)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7562)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7562
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1565065"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..224
                     /locus_tag="VNY06_00415"
     CDS             <1..224
                     /locus_tag="VNY06_00415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX13337.1"
                     /translation="ALLGMERRRLMNLWPFLLLLPVYYLLGSVAAWMALYDLISRPYH
                     WHKTEHGLAKTSRQRVPMEREITADALIR"
     gene            complement(520..3192)
                     /gene="ppdK"
                     /locus_tag="VNY06_00420"
     CDS             complement(520..3192)
                     /gene="ppdK"
                     /locus_tag="VNY06_00420"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020176621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050242 - pyruvate, phosphate dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HWX13338.1"
                     /translation="MAKWVHIFGRGAAEGERGSSDLLGGKGAGLAEMSRLGLPVPSGF
                     ILTTEVCSYFYNNGQQFPAELADEVEEALDFISQATGHRFGDEQDPLLVSARSGAKFS
                     MPGMMDTVLNLGLNDIAVEALARRSGDERFAFDSCRRFIQMYASVVLGIEHHIFEEIL
                     ECYKENKGATLDTELSAGDWKKLVAEFKSTVEKNLSTPFPQDPRQQLWGAVGAVLNSW
                     MNPRAVNYRRLHAIPENLGTAVTIQAMVFGNMGETSATGVAFTRNPSTGAKELYGEFL
                     LNAQGEDVVAGLRTPQYITEAARIAAAAPEISMEAAFPEIFAEFVSTADLLEKHYRDM
                     QDMEFTVERGKLWMLQTRRGKRTTKAALRIAVDLTNEGLISKEEALGRVDPPSLEQLL
                     HPTLDPRAHRQIVATGLPASPGAASGEIVFSASDAEQMKSAGRKVILVRIETSPEDIM
                     GMNAAEGILTTRGGMTSHAAVVARGMGKPCVSGAGTIRVNYANAMMTVAGLTFKKGDL
                     LTIDGSTGQVIKGAIAMRQPELSDEFAVMMGWADEVRRMGVRANADTPRDARVARKFG
                     AEGIGLCRTEHMFFEGERIVAVREMILAEDAAGRGTALAKLLPMQRADFAELFEIMPG
                     LPVTIRLLDPPLHEFLPCGEAEIATVAKEMSVLPEKLTGRAAELQESNPMLGFRGCRI
                     AIAYPEIAEMQARAIFEGAVAAKRKTGRAVHPEIMTPLIIARTEFDLIKTQIDAMAKS
                     VADETGETIAYSVGTMIETPRACLRAGELAAAADFFSFGSNDLTQTCLALSRDDAATF
                     LGDYTAKGLLLANPFATIDIEGTGELIEIAVTRGRAVRPGIKIGICGEHAGDPASIDF
                     CEKVGLTYISCSPFRVPAARLAAAQAALTRKAQH"
     gene            complement(3302..3763)
                     /locus_tag="VNY06_00425"
     CDS             complement(3302..3763)
                     /locus_tag="VNY06_00425"
                     /inference="COORDINATES: protein motif:HMM:NF015674.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HWX13339.1"
                     /translation="MGVAALILVTSATIASVYTIAIFMILAGGAEIASSLSAKTWGRF
                     VLWIAAGLAYIVVASFALAQPLMAAAFFTLLLGAAMIATGVVRIFLGAHLGAPSRTPV
                     LFAGLLTLWVGMLIIAGWPADRFLILGVLLGLDLLFWGVAWIRLGLRLRNL"
     gene            4455..6404
                     /gene="dxs"
                     /locus_tag="VNY06_00430"
     CDS             4455..6404
                     /gene="dxs"
                     /locus_tag="VNY06_00430"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012384796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HWX13340.1"
                     /translation="MKEELVTKKSKTPLLDAISTPADLRRLPEVDLAQVANELRIETI
                     DAVSVTGGHLGAGLGVVELTVALHYVFDTPNDRLVWDVGHQAYPHKILTGRRDRIRTL
                     RRGGGLSGFTKRSESEYDPFGAAHSSTSISAGLGMAVANTLSAKKTHVISVIGDGAMS
                     AGMAYEAMNNAGSMHSRLIIILNDNDMSIAPPAGALSAYLARLVSGGTYRSVRETAKQ
                     LGRHLPKFIYDRARKTEEFARNFWAGGTLFEELGIYYVGPIDGHNIDHLLPVLKNVRD
                     HDIGPILVHVVTQKGKGYAPAEASTDKYHGVNTFDVVTGVQVKPKANAPSYTRVFADA
                     LLAEAHQDDKIVAITAAMPSGTGLDLFQKVFPERTFDVGIAEQHAITFAAGLATERYK
                     PFCAIYSTFLQRAYDQVVHDVAIQKLPVRFAIDRAGLVGADGATHAGSFDIPYLGILP
                     GMVVMAAADEAELVHMVATAAAYDEGPIAFRYPRGEGVGLDLPERGAVLKIGRGRILR
                     EGTKVAILSFGARLAEALKAADDLATYGISTTVADARFAKPLDRELLFRLAAEHEVLI
                     TIEEGSIGGFGSFVMQALSEVGVFDGVGARSLKFRAMMLPDCFLDHDKPEKLYASARL
                     DAKGIVAKALATLGRATDARKCLIA"
     gene            6611..>7562
                     /locus_tag="VNY06_00435"
     CDS             6611..>7562
                     /locus_tag="VNY06_00435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HWX13341.1"
                     /translation="MAVRISFAFRVAAVPVLLAVIAIVETRHPFNMFRLVSLGVVFFL
                     LVDIASLLRGNWRDFLLVLTSLAFGIFLVEAAANILEPKQSTVVSPEGLYGPRPAIGW
                     GPGHPGRFHAEKTDPKTGATIYSVDYTIDSNLLRQTLSCETGPTVVFFGCSFTFGEGL
                     NDSATLPQAFVDSLDRRERVLNLGFSGYGPQHFLSELQSGILDSVIGPQPKLFVFLTS
                     AGHAERSACKPYWARHGPRYRIENGQLILKGACYEGLGLWLLDWLESSAAYRWLIEPY
                     RRRVSHDDIELYLQITLAAVNFAKTKYGVPVILLYFKVEGD"
BASE COUNT         1609 a         2166 c         2106 g         1681 t
ORIGIN      
        1 ttgcgctgct tggcatggag cggcgcagat tgatgaacct ttggccattc ctgctgctgc
       61 taccggttta ttatttgctc gggtcggtcg cggcgtggat ggcgctttat gatctcattt
      121 cgcggcccta ccattggcat aagaccgagc acggcctggc caagacctca cggcagcgcg
      181 tgccaatgga gcgcgagata acggcggatg cgctaatcag atagagttct gtggaaacgc
      241 ataaggatag gtttcagtcg ctcgcagctt atgaagttag aaaaggttaa ttcataaagg
      301 gggtggacta aaaatgtcag tcgcgcgaaa tcgcaactgg ccgttgatcg atcggctggg
      361 catttggcgg cggtttcctg ccaaacgtgc gaatcattaa ctactgccga cgcaagcttc
      421 ggctgccgtc cttgacccat caaagccggg tcgccgacaa tcgaacatga atggagcccc
      481 ctgaccttgc ccgttctggc gaatccagaa ttcaacggtt taatgttgag ccttgcgcgt
      541 gagagcggct tgcgccgctg cgagccgtgc ggcagggacg cggaagggcg aacaggaaat
      601 atatgtaaga cctaccttct cgcaaaaatc gattgacgcc ggatcgcccg cgtgctcacc
      661 gcagattcca attttaatac ccggccgcac cgcacgtcct cgcgtcacgg caatttcaat
      721 tagctcgccc gtgccctcta tatcgatggt cgcgaaagga ttggcaagaa gcagtccctt
      781 ggccgtgtag tcgcctagaa acgttgcagc atcatcgcgc gaaagcgcaa ggcaggtctg
      841 agtgagatca ttgctgccaa acgaaaagaa gtcggccgca gcagcgagtt cacccgcgcg
      901 caaacaggcg cgcggcgtct cgatcatcgt gccaacagaa taggcgatag tctcgcccgt
      961 ctcgtcggcg acggactttg ccatcgcatc aatctgcgtc ttgatgaggt cgaactcggt
     1021 ccgagctatg atgagcggag tcatgatctc cggatgcacc gccctgcctg tcttgcgttt
     1081 ggcggccacg gccccttcga aaatcgcccg agcttgcatt tcggcgatct cgggataagc
     1141 gatggcgatc cggcaaccgc gaaaccccag catcggatta gattcttgga gctcagcggc
     1201 gcgccccgtg agtttttccg gcaagacact catttccttt gccaccgtcg cgatctccgc
     1261 ctcgccacac ggcaagaact catgcagcgg cgggtcgagc agccggatcg tcacaggcag
     1321 ccccggcata atctcgaaca gttcagcgaa atcggcgcgt tgcatcggca aaagcttggc
     1381 aagcgcggta cctctgccgg ctgcatcctc cgcaagaatc atttcgcgca cggcgacaat
     1441 gcgctcgccc tcaaaaaaca tatgctcggt gcggcagagc ccaatccctt ccgcaccgaa
     1501 tttccgcgcg acgcgcgcgt cgcgcggggt atcggcatta gcccgcacgc ccatgcggcg
     1561 cacctcatcg gcccatccca tcataacagc gaattcatcc gaaagctcgg gctggcgcat
     1621 tgcgatggct cccttgatga cttgaccagt gctgccatca atggtcagca gatcgccctt
     1681 tttgaaggtt aggccagcca ccgtcatcat cgcattggca tagttgacgc gaatcgtgcc
     1741 cgcgccggag acacagggct tgcccatgcc gcgcgcgaca accgctgcgt gggaagtcat
     1801 gcccccgcgg gtggtcaaaa ttccttccgc cgcattcatc cccataatgt cctcggggct
     1861 tgtctcgatg cgcacaagga ttaccttgcg gccggcgctc ttcatttgct cggcgtcgct
     1921 cgcagaaaaa acaatctctc cgctagctgc gcctggcgaa gctggcagcc cagtggctac
     1981 aatctgacgg tgcgctctgg gatcgagcgt cggatgtaaa agctgctcaa gcgagggagg
     2041 gtccacgcgc ccaagcgcct cttctttgct gatcaggccc tcattcgtta aatcaacagc
     2101 tatacggagc gcggcttttg tggtgcgctt gcccctgcga gtttgtagca tccaaagttt
     2161 gccgcgctcg acggtgaatt ccatgtcctg catgtcgcgg taatgctttt cgagaagatc
     2221 ggccgtgctc acgaattcag cgaagatctc cgggaatgcg gcctccatgg atatttctgg
     2281 tgccgccgcc gcgatgcgcg cggcttcggt gatatactgc ggcgtcctta gtcctgcgac
     2341 aacgtcctcg ccttgcgcat tgagcaggaa ttcgccgtaa agttcctttg ctccggtcga
     2401 cggattgcgc gtgaaggcga ctcctgttgc cgatgtttcg cccatattgc caaacaccat
     2461 ggcctggatc gtcaccgcgg tgccaagatt ctccgggata gcatgcagcc gccggtaatt
     2521 gaccgcgcgt gggttcatcc aggaattgag cacagcaccg acggcgcccc agagctgttg
     2581 gcgcggatct tgcggaaaag gcgtcgaaag gttcttttcg accgtgctct tgaactcggc
     2641 cacgagcttc ttccaatccc cggccgatag ctccgtatca agagtggcac ctttgttttc
     2701 tttataacat tctaggattt cctcgaaaat atggtgctcg atacctagca ccacgctcgc
     2761 atacatttgg atgaaacggc ggcacgagtc aaaagcaaag cgttcgtcgc cggagcgtct
     2821 tgccaaggcc tcaacagcaa tgtcgttgag accaaggttc aaaacggtat ccatcatccc
     2881 aggcattgag aattttgccc cggaccttgc cgagacgagc agcggatctt gctcgtcacc
     2941 aaagcggtgt ccggtcgcct ggctgataaa gtcgagcgct tcctctacct cgtcagcaag
     3001 ctcagcggga aactgctgtc cattgttata aaaataactg cagacctcgg tggtaagaat
     3061 aaagcccgag ggaaccggca acccaagcct tgacatttcg gcaagaccgg cgcccttgcc
     3121 gccgagaagg tcgctgctgc ctctttcgcc ctcggctgcg ccccggccaa aaatatgaac
     3181 ccactttgcc atgttagacc ttttgcgaca ccaaaacgct cgcgagaagc ctccgtccag
     3241 cgtgatcagc aatggggctt ttgaagataa gtcctcgcca ttggcgcact cgattgcttg
     3301 ttcaaaggtt gcgcaagcgc aacccgagcc ttatccaggc aacgccccaa aacaagaggt
     3361 caaggccaag gagaacgccc aagattaaaa acctgtccgc tggccagcca gcaataatca
     3421 acatcccaac ccataaggtt aataggccgg caaacaaaac gggggtgcgt gagggcgcac
     3481 caagatgcgc accgaggaaa atccgaacca cacccgtggc gatcattgcg gccccgagca
     3541 gcaaggtgaa aaaagcggcc gccatcaaag gttgagcaag agcaaaggac gcaacgacaa
     3601 tataggctag cccagcggct atccaaagaa cgaaccggcc ccaagtcttg gcgctcagac
     3661 tggaagcaat ttcggcaccg ccagcaagga tcatgaaaat tgcgattgtg tagactgagg
     3721 caatggtcgc cgaggtgaca agtataagcg cggcaacgcc catgcggcaa tcacggcgcc
     3781 caaagcaacg atccagcccc aacggggctt gaggctcgaa atcgcccttt tcacgtcttg
     3841 cggctggtct tgaaacggcg cgggatcgtt ggaaaaaatc actcctgaat tcccttaggt
     3901 cagggccaga cgtgcgatga tagcttgcag cggccccaaa accggtcgaa agctccatct
     3961 ggtggtgcca cacgagaact cttgttctgc ttgactatac aactcaactt tgcttggaga
     4021 aaagcctgct ttccatttcg cgcttgtaag cttggatccg ctacacaccg cgcaaccatt
     4081 cagcggcgtt gacggttccg acggcctcga ttgtgcttgg gggatcgcgg ctagcgcgca
     4141 tgttgaaaac ttactattgc cccagtccaa aggaaaagcg cgcggaagct gattgccggc
     4201 cccttggatt catatgccct tcggcaacgc aaaggttagc cgaatctcta accgactgtg
     4261 atgcgaaagt cgcggccgca aagaaaagct gccaaacgct tgcccaaaaa caggctagcg
     4321 ttgcgggtta cggttttgtt ggctattcac cttgagcttc tggaatgaga cgattcccta
     4381 ttcttcaatc caggagcgtt ttaagtattt cggcctatcg gattctgagc acggcttttt
     4441 cgagtcaggc gttcatgaaa gaggaactag tgacaaaaaa atcaaagact cctttgctcg
     4501 acgcaatttc aacgccagcg gacttgcggc gcttgcccga agttgacctc gcccaagtcg
     4561 cgaacgaatt gcgcatcgaa acaatcgatg cggtttcggt gacgggcgga catcttgggg
     4621 cggggcttgg tgtcgtagag ctcacggttg ccctgcatta tgtctttgat acaccaaatg
     4681 accgtctggt gtgggacgtt ggccaccagg cctatccgca caaaatcctt actgggcggc
     4741 gagaccggat tcggaccctg cgccgtggcg gaggcttgtc ggggttcacc aaacgctccg
     4801 aaagcgaata cgacccattt ggcgctgccc actcatcgac ctcgatctcc gccgggctcg
     4861 gcatggcagt ggccaataca ctctctgcca agaaaaccca tgtcatctcc gtaattggcg
     4921 atggcgcaat gtctgctggc atggcctacg aggccatgaa caacgcaggg tccatgcatt
     4981 cgcgcctcat catcattctc aacgacaatg acatgtcgat cgctccacca gcaggagccc
     5041 tgtcggccta tctcgcccgg cttgtctctg gcggcaccta tcgttccgtg cgcgaaacgg
     5101 caaaacagct tggacgccat cttcccaaat tcatttatga tcgtgcccgc aagaccgagg
     5161 aattcgcccg caatttctgg gctggcggaa ctttgttcga ggagcttggt atttattatg
     5221 ttgggccgat cgatggacac aatatcgatc acctgctgcc ggttttgaag aatgtccggg
     5281 atcacgatat cgggccgatt ttggtgcatg tcgtaaccca gaaggggaag ggctacgcgc
     5341 cagccgaggc gtcaaccgac aaatatcatg gcgtcaacac tttcgacgtc gtcacggggg
     5401 tccaggtcaa acccaaggcg aacgcgccat cctatacgcg agtcttcgcc gatgccttgc
     5461 tcgccgaggc gcatcaggac gataagattg ttgcgatcac cgccgccatg ccaagcggca
     5521 ctggccttga tcttttccaa aaggtctttc cggaacgcac ctttgatgta gggatcgcgg
     5581 agcagcacgc cataactttt gcggccgggc ttgccacgga acgctacaag cccttctgcg
     5641 cgatctattc gaccttcctc cagcgcgcct atgatcaggt cgttcacgac gtcgcgattc
     5701 aaaaattgcc tgtgcggttc gcgattgatc gcgccggcct cgttggcgcc gatggcgcaa
     5761 ctcacgctgg ctctttcgac attccctatc tggggattct tcccggcatg gtcgtgatgg
     5821 cggcggcgga cgaagccgag ctcgtgcaca tggtggcgac ggccgcggct tatgacgaag
     5881 ggcctattgc cttccgctat ccgcgcggcg aaggcgttgg cttagacttg cctgagagag
     5941 gggcggttct gaaaattggc cgcggccgga ttttgcgcga gggcaccaag gtcgctattt
     6001 tgtcgtttgg cgcgcgcttg gctgaagcgc tgaaggccgc cgacgatctt gccacctatg
     6061 ggatctcgac gacggtggcc gacgcgcgct tcgccaagcc tctcgatcgg gagctccttt
     6121 tccgcctcgc ggcggagcat gaggtattaa tcacgattga ggaaggctcc atcggtggtt
     6181 tcggctcatt tgtgatgcag gctttgagcg aagtcggagt ttttgacggt gtcggtgcga
     6241 ggagtttgaa attccgcgcg atgatgttgc ccgactgctt cctcgatcac gacaagccgg
     6301 aaaagcttta tgcttctgcc cgccttgacg ccaaaggtat tgtggccaaa gcccttgcaa
     6361 ccctcggccg cgctacggac gcgaggaaat gcctgatcgc gtgattggtg attggagggc
     6421 ctccatccgg tcttctcgtc agtctcaacg gttgatttga acttgcgagc agggcaagtt
     6481 ggttatgcag gtttggctgc gccatgtaac gcctactggg cgtcgcttga aatttccaaa
     6541 caatgctcaa gccgcaatcg ggatctcatc ttgcgtccct aaccccgcaa ctcacagaga
     6601 gctcggtgaa atggcggtaa gaatttcctt tgccttccga gtagcggcag tgccggtttt
     6661 gctcgcggtt attgcgattg ttgaaacgcg gcatccattc aatatgttcc ggctggtcag
     6721 tttgggtgtg gttttttttc ttcttgttga tatagcatca cttcttcgtg gcaattggcg
     6781 cgattttctt ctcgtgctga catcgcttgc ttttggcatt ttcctcgtcg aggctgcggc
     6841 caatattttg gagcccaaac aatcgactgt tgtgtcgcca gaggggctct atgggccgcg
     6901 gcccgctatc ggctggggac caggtcaccc ggggcggttt catgctgaaa aaaccgatcc
     6961 aaaaacgggt gccactattt atagtgtcga ttacacaatc gattcgaatc ttctgcgcca
     7021 gacattgtcg tgcgagaccg gtcccacggt cgtttttttt ggctgctcgt tcactttcgg
     7081 ggaagggttg aacgattccg ccacattgcc acaagctttc gtagatagtc tcgatcgtag
     7141 ggagcgcgtt ctgaatctcg gtttctcagg ttatgggccg cagcatttcc tgagcgaact
     7201 acagtccggg attttagatt ccgtgatcgg gcctcagcca aaactatttg tatttttgac
     7261 ctcggctggg cacgccgagc gcagcgcctg caagccctat tgggcgcgcc atgggccgcg
     7321 ttacagaatc gagaatggcc agctaatact gaagggtgcc tgctatgagg ggcttggcct
     7381 atggttgcta gattggctcg agagttcggc cgcctaccgc tggctcatcg agccctatag
     7441 gcgcagggtg agccatgatg atatcgaact ctacctccag atcaccctgg ccgcggtaaa
     7501 tttcgccaag accaaatatg gcgttcccgt gattctcctc tatttcaaag tagaagggga
     7561 tg
//
LOCUS       DATMPU010000021         3330 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_3116577,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000021 DATMPU010000000
VERSION     DATMPU010000021.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 3330)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3330)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3330
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3116577"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..784)
                     /locus_tag="VNY06_00440"
     CDS             complement(<1..784)
                     /locus_tag="VNY06_00440"
                     /inference="COORDINATES: protein motif:HMM:NF039236.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HWX13342.1"
                     /translation="MFLRNYWYVAATDAEVGRKPLSRTILGEPIVLFRTEAGAPVAME
                     DRCAHRHLPLSMGKLVGDELQCHYHGLRFGTDGRCTRIPGQEQIPRGAKVKTYPVAER
                     YHWIWIWMGDPALADPAAITDFHWLDDPNHWGAKASYLHVEANWQLVVDNLLDLTHLA
                     FVHDTTIGNLALAEHAKVQVTRAPNNVVVTRWIIDQEPPPTFKKVGGFTGKVDRWQII
                     DFVPPAFLRLDVGATPTGTGAPEGKRVGGIGMRNLNAITPETE"
     gene            1690..1977
                     /locus_tag="VNY06_00445"
     CDS             1690..1977
                     /locus_tag="VNY06_00445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX13343.1"
                     /translation="MITADVRLIMAERPPINPGDWITVGEIRAVVSAVREPDNFSGDC
                     EVVFNPQKPTNHDVVWTGEKWRFAERSDFGGYADKYPRLRQYVSILKRGPR"
     gene            2155..>3330
                     /locus_tag="VNY06_00450"
     CDS             2155..>3330
                     /locus_tag="VNY06_00450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX13344.1"
                     /translation="MPTNDKVVRALESISRIPEADAGEWLSSAEKSIEFLKQNVQSDR
                     LVLFASMPCVLIHVVLAPLRNLNPPDQEDLTRDFVSPDEAWAIQHVWGGGKPDRVYLS
                     PPLAGRKTLKDGEKLYFSRSFAGSRRRSIEISQKLVHALDLHFIEERNAYCRLDDDGD
                     LEDVISITEKDKDSWAEHLTVVTIQMRDFVEYMRLSDMGMVIFFDFTRVDHRSFHGWS
                     DQNRFDRKAADLFYNGGVMPGHASYVNGRMIARPSITMEDIVRAKTEASDPAFRRYTT
                     FKAIDLKTGDRIETSCDPEGLSNYFQPNSPLPLQMSPVFFDAEVLHKYKADTEKYTLE
                     DRSINCRGTWSLQTYDINEAGQVHTYLRYLGYLPYKEQLYWQSFNEWPKAPLSERAIA
                     "
BASE COUNT          725 a          939 c          975 g          691 t
ORIGIN      
        1 tttcggtctc cggcgtgatg gcgttgagat tgcgcatgcc gatgccgcca acgcgcttgc
       61 cttccggcgc gccggtgccg gtcggcgtgg cgccgacatc gagccgcagg aacgccggcg
      121 gcacgaagtc gatgatctgc cagcggtcga ctttgccggt aaaaccgccg actttcttga
      181 aggtcggcgg cggctcctga tcgatgatcc agcgcgtcac cacgacattg ttcggcgccc
      241 gggtgacctg caccttggcg tgctcggcca gcgccaggtt gccgatcgtg gtgtcgtgga
      301 cgaaggccag atgcgtgaga tcgagcagat tgtcgaccac gagttgccaa ttggcctcga
      361 cgtggaggta ggaggctttg gcgccccaat gattcggatc gtcgagccaa tggaaatcgg
      421 taatcgccgc cggatcggcc agcgccggat cgcccatcca gatccaaatc cagtgatagc
      481 gctcggcgac cggataggtt ttgaccttgg cgccgcgcgg aatttgctcc tggccgggaa
      541 tgcgggtgca gcggccatcg gtgccgaagc gcagcccgtg ataatggcac tgcaattcgt
      601 cgccaacgag cttgcccatg gacagcggca gatggcggtg cgcgcaacga tcctccatcg
      661 ccaccggcgc gcctgcttcg gtgcggaaga gcacgatggg ctcgcccaaa atggtgcgag
      721 acagcggctt gcggccgacc tcggcatcgg tcgcggcgac gtaccagtaa ttgcgcagga
      781 acatggcggt tctccctgct gccgatatta tcctctcgcg cgcccgcgcg ggagggggcg
      841 ccgctctttt ctgccccgcg ccagcgggag aggaaagaca agcgctttcg ttctcgcgga
      901 aagcgcgagc gcatgcggaa caacgaatgc cacgaaagac tagcctccca acaagaaggg
      961 aggcggagcg ccgagaagcg cacctaccag gagccgcatc gacaagcgat gcggcgcgcg
     1021 ccctctctcc tgtcttccca ctgcgggagg accgaggccc aggacttgcc ttggacctca
     1081 aaaaatggag cgggcgcgct cgcctttcgg caccccaccg cggcatccga ggtttttgcc
     1141 ctcggctcgg ctcaggccgc gcttcctgga accaccggat gcaaacggga ggaccctctc
     1201 cggcaccagt gcagcgagca cctcgcggtc cgacacgcgc cggacgggac gatgcccaag
     1261 ccgcccgcga gcgcagtgta tcgctacgcc cacgagaacc gctcccgctc cgccctaagg
     1321 agtaccctcg cgaaaggcgt ccctttgcga gcgggatttt ggatatgtaa ctgcaatggg
     1381 gacagatgtc aagggcaatg tctctttatt agggacgcgc catcggccgg attagcgcgc
     1441 cgtaatctgc cctccgattg ccgagaggcg gcggattagg ccttcggccc atccgtccta
     1501 ccgtgcaatc ggctttgcga tgtggcgccg gcggtgcggt cgctcgctca agtttccggt
     1561 gggtgttgag gcggcgttca tgagaattcc ggttggttga tcggatttct acttagcccc
     1621 ttcaacacat gatgaaaagc gattcgagaa gattgacgga cgaagtgtac gaccgataaa
     1681 gaggtgccga tgatcactgc tgatgtgcgc cttatcatgg ccgagagacc tcctatcaat
     1741 cctggagatt ggatcacagt cggggaaata cgcgccgttg tgtcggcggt acgagaaccc
     1801 gacaattttt ccggagactg cgaggttgtt ttcaatccgc aaaagccaac caatcacgat
     1861 gtagtttgga cgggcgaaaa atggcgtttc gccgaaagat ccgatttcgg cggatacgca
     1921 gacaaatatc cacgccttcg acaatacgtg tcgatcttaa agagaggacc tcgataaacc
     1981 cacaagaaga aggaacggtc cgctcgtcca gcaaacgtca gcccgtagga tgggttgagc
     2041 gtagcgaaac ccatcgcctc gctcgcacta ttacgcccat caggacgcga tgttgcgaat
     2101 tttctatcgc gggagcaaat tcgctttcgt tcttcaatag gctcggttct gcgcatgcca
     2161 accaatgaca aggtagtgcg agcgctcgaa agcatttctc ggattcctga ggcggatgcc
     2221 ggcgagtggc tatcttcggc agagaaatcg atcgaatttc tcaagcagaa cgttcaatca
     2281 gaccgattgg tgctattcgc ttcaatgccg tgtgtgctca ttcacgtcgt tcttgcaccc
     2341 ttaaggaatc tcaatcctcc ggatcaagag gatttgaccc gcgatttcgt ctcgccggat
     2401 gaggcttggg caattcaaca tgtgtggggt gggggaaagc ccgaccgggt atacctatcc
     2461 ccacctttgg ccgggcgtaa aacacttaag gacggtgaaa aactgtattt cagccgatca
     2521 ttcgccggtt ctcggcgaag atcgatcgaa attagccaga agctcgttca cgctctcgac
     2581 cttcacttca ttgaggagcg gaacgcctat tgccgcctcg atgacgatgg tgatttagaa
     2641 gatgtcatct ccattactga aaaagataaa gatagttggg cggagcacct taccgtagtg
     2701 acgatccaga tgagggattt tgtggaatac atgcgcctca gcgatatggg aatggtgatc
     2761 tttttcgatt tcacgcgggt ggaccaccgc tcgtttcacg gatggtccga ccaaaaccgt
     2821 tttgatcgta aggctgctga tttattttac aacggcggag tcatgccagg gcatgcaagt
     2881 tatgtgaacg gccggatgat cgctcgaccg tccattacta tggaagatat tgtccgggcc
     2941 aaaaccgaag caagcgaccc ggctttccgc cgctacacca cattcaaagc gattgatctg
     3001 aaaacaggcg accggattga gacttcttgt gatccagagg ggctatcaaa ctatttccag
     3061 ccgaactcgc ctctgcccct gcaaatgtcg ccggtctttt ttgatgcgga agttctccac
     3121 aagtataaag ccgatactga aaaatacact cttgaggacc gctcgatcaa ttgcagggga
     3181 acgtggagcc tccagaccta tgatatcaac gaggccggac aagttcatac ttatctgcgg
     3241 tacttgggct atctaccata taaggagcag ctgtattggc agtcatttaa tgagtggcca
     3301 aaggcgccct tatcggagcg agcaatcgca
//
LOCUS       DATMPU010000022         2847 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_3736925,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000022 DATMPU010000000
VERSION     DATMPU010000022.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 2847)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2847)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2847
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3736925"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..244)
                     /locus_tag="VNY06_00455"
     CDS             complement(<1..244)
                     /locus_tag="VNY06_00455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX13345.1"
                     /translation="MANFLARPAAAIGAMLAFATVPLHLYLSKAHSEQFAAMLLAGVG
                     AVYIGFGLQKGNQSQIATEILVAIDFFAAALGGLWVS"
     gene            439..1464
                     /locus_tag="VNY06_00460"
     CDS             439..1464
                     /locus_tag="VNY06_00460"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003614060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046167 - glycerol-3-phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HWX13346.1"
                     /translation="MPPDRKNRIAVLGAGAWGTALANLAARGGGVDDTEVMLWARDPE
                     HVAEIRATGINARYLPGVPLLSNVNPISESAQVQGADLFLAAVPAQALREVLETFAPE
                     LAEGVPVIVCAKGIERTTGQFLSEVVSSVLPNNPPAVLCGPSFAREVAEGLPTAVTLA
                     AHDGDLAAKLAGMLAAPSFRLYSSSDIRGVEIGGAAKNVLAIASGITAGRGLGASAAA
                     ALIARGFAELSRFGRAFGAETATLVGLSGLGDLVLTCTSLMSRNYSLGVSLGRGLGVA
                     EALARGGLCEGVHTCGALLDLAHARDVDMPIAAAVDAVLAERITIEDSIAGLLARPSK
                     AEFEERP"
     gene            1553..1966
                     /locus_tag="VNY06_00465"
     CDS             1553..1966
                     /locus_tag="VNY06_00465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019404928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EVE domain-containing protein"
                     /protein_id="HWX13347.1"
                     /translation="MAYWLLKSDAGEWSWQDQIKAGQKGTSWNGVRNHSAKLNLMAMK
                     LGEQAFFYHSNEGRAIVGIVEIIKPYYPDPTDRSGKFGMVDVRAVEALKEPVTLDAIK
                     QDPRLANMVLVNNSRLSVQPVSYEEWKIIRAKGGL"
     gene            2166..>2847
                     /locus_tag="VNY06_00470"
     CDS             2166..>2847
                     /locus_tag="VNY06_00470"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HWX13348.1"
                     /translation="MTPKTDKASNHWPLLDFLRATAALLVLLTHTRFSYFPNIDFADQ
                     TNVFLKLFYFITNMGHEAVAIFFVLSGFLIGGSLTDSTLRGSFDLPRYLIARFVRIFI
                     VYLPALVITQGVFLFGLLLLSDPGDAPLSGHRQFGGVSKAICYLSGLQGFSCPAWGKN
                     PALWSLGYEWALYLFAPAIIQLIVWKASLGLRLLAVALVCAIAVTVCHYPTDAVFWFG
                     AWFVGAGSY"
BASE COUNT          559 a          822 c          816 g          650 t
ORIGIN      
        1 cactcaccca gagacccccc aatgcggcag caaagaagtc tatcgcaacc aatatctcgg
       61 ttgcaatttg ggactgattg cctttttgca gcccgaatcc gatgtaaacc gcgccaactc
      121 ccgccaaaag cattgcggcg aattgttccg aatgtgcctt cgacaaatat aaatgcagcg
      181 gaaccgtagc aaaggcaagc atcgcgccaa tcgcggccgc cggccgtgca aggaaatttg
      241 ccatgggctg tgatccattg cctaagagcg tattggctgc gctctgacct caccccctga
      301 acgagcaaag tgcagccttt gcaatgcggc cctggcaaag gctatctgtt gttgtgaagt
      361 ttccatagca agccgccacg cgaaccacaa cattggtttc gcggaaggca tcaatccgcg
      421 gaatgaggac aaaaaaggat gccgccggac cgcaaaaacc ggattgccgt tctcggggcc
      481 ggggcctggg ggaccgccct tgccaatctc gccgcgcgcg gcggcggcgt ggacgatacc
      541 gaagtcatgc tttgggcgcg tgatcccgag catgttgcag agatacgcgc aaccggtatc
      601 aatgcaagat atctgccggg tgttccgctt ctctcgaatg tcaatcctat ttcggaatca
      661 gcacaggttc agggcgcgga cctctttctt gcggccgtcc cggcacaggc gctgcgcgag
      721 gtcctcgaaa ccttcgcgcc ggagctcgca gaaggggtgc ccgtcattgt ttgcgccaag
      781 ggtatcgagc gcacaaccgg acaattcttg agcgaagtcg tctcttctgt gttgcccaac
      841 aatccgcccg cagttctttg tggtccaagc tttgcgaggg aagttgctga agggctgccg
      901 accgcggtca ctctcgccgc gcacgacggc gatctcgcgg cgaagctcgc gggcatgctg
      961 gcggctcctt ccttccggct ctattcatcg tccgacataa gaggcgtcga aatcggcggg
     1021 gcggcaaaaa acgtcctcgc aattgcaagc gggatcactg ctggccgcgg ccttggagcg
     1081 agcgccgcgg ctgcccttat cgcccggggc tttgccgagc tcagccgttt cggccgcgcc
     1141 tttggggccg agaccgccac tttggtcggc ctttcgggcc ttggcgatct ggttctcact
     1201 tgcacttctt tgatgtcgcg aaactactcc ctcggcgtct cgttggggcg cggattgggt
     1261 gttgcggaag ccttggcgcg tggggggctc tgtgaaggcg tgcacacctg cggcgcgctc
     1321 ctcgatcttg cccatgccag ggatgtagac atgccgattg cggcggctgt ggatgccgtg
     1381 cttgccgagc gaattacgat cgaggactct atcgccggcc tgctggcacg gccctcaaaa
     1441 gccgaattcg aagaacgccc ttaatcgaga aaccgtctcg attttcgatc aattgacgtc
     1501 actgtgcctt atgatgagtg aaaacccatc acaaaaaaac gcgagggaga aaatggccta
     1561 ttggctgctc aaaagcgacg ccggagaatg gtcctggcag gatcagataa aagccggcca
     1621 aaaggggacc tcatggaatg gcgtgcgcaa tcatagcgcc aagctcaatt tgatggcgat
     1681 gaagcttggc gaacaggcct ttttctatca ctccaatgag ggcagggcga ttgtcggcat
     1741 cgttgagatc atcaagccct actatcccga tcccacagat cggagcggca aattcggcat
     1801 ggtcgatgtt cgcgcggttg aggcactgaa ggagcccgtc acgctcgatg ccatcaaaca
     1861 agatccgcgt ttggcaaata tggtgctcgt caacaattcg cggctttcgg tgcagccggt
     1921 gagctatgag gaatggaaga tcatccgcgc caaaggcggc ttatgatgcg ctgggacgcg
     1981 agggacgccg cgctcgtcgg gtccatcggc cagaaaatcg acgttcttat gtcgccctac
     2041 attcttccta gctagacgcg aaccgcttgg ctactgacgg ggccttgact ttgcgcgcgt
     2101 cagccgatga ggtgcgtgct ccaggggcga ttggaactca tgccaacttc tgacaacgag
     2161 gggcgatgac cccaaagacc gacaaagcgt caaatcattg gccgctgctc gattttctgc
     2221 gcgcgacggc tgcgctcctt gtattgctca cgcatacccg cttttcctat tttcccaata
     2281 ttgacttcgc cgaccagaca aatgttttcc tgaagctgtt ttatttcatc acaaacatgg
     2341 gtcacgaggc tgttgccatt ttttttgtgc tcagcggttt cctgatcggg ggaagcctca
     2401 ccgattccac gcttcggggc agttttgatc ttccacgtta cctcattgcg aggtttgttc
     2461 gcatctttat cgtctacctc ccggcactgg tcatcaccca gggagtattt ttgtttggat
     2521 tacttctgct tagcgatccc ggagacgcgc cgctgtccgg ccaccgacaa ttcggcggcg
     2581 tttcgaaggc aatttgctat ctctccggat tgcagggctt cagttgcccc gcttggggca
     2641 aaaatcctgc cctgtggtca cttggttacg aatgggcgct ctatctgttc gcccccgcga
     2701 tcatccagct gatcgtgtgg aaggcttctc tcggtcttcg cctgttggca gttgcgcttg
     2761 tctgcgcgat cgcggtgacg gtctgccact atcccactga tgcagtgttt tggttcggtg
     2821 cttggttcgt tggcgcaggc tcatacc
//
LOCUS       DATMPU010000023         3789 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_1877830,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000023 DATMPU010000000
VERSION     DATMPU010000023.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 3789)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3789)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3789
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1877830"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(55..210)
                     /locus_tag="VNY06_00475"
     CDS             complement(55..210)
                     /locus_tag="VNY06_00475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX13349.1"
                     /translation="MDAKTRDLALLAALSFICTGVAWIWGYVILGSVLFALGGVAAIG
                     LAENHWR"
     gene            complement(534..896)
                     /locus_tag="VNY06_00480"
     CDS             complement(534..896)
                     /locus_tag="VNY06_00480"
                     /inference="COORDINATES: protein motif:HMM:NF025005.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KTSC domain-containing protein"
                     /protein_id="HWX13350.1"
                     /translation="MIGATLAGTGMIVAILLSSPAGAEEVCVKYRKCLNIDQFKCEII
                     TRNSFINRVCYLEANTIIKLKDTYYHHCSVKPETMMEFLAAPSMGSYYNKNIRSKPDG
                     THGPFDCRDHLIPTVSIL"
     gene            complement(937..1140)
                     /locus_tag="VNY06_00485"
     CDS             complement(937..1140)
                     /locus_tag="VNY06_00485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX13351.1"
                     /translation="MDRGNDAKTTRDLASLAVLSLFCAGVALISDHLVLGLVFFVLGG
                     LCGDALGRDGGIRQELAAGLQDV"
     gene            complement(1163..1681)
                     /locus_tag="VNY06_00490"
     CDS             complement(1163..1681)
                     /locus_tag="VNY06_00490"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HWX13352.1"
                     /translation="MKDGVLPVNAPAPNADALFEQAQAAEAAGDIAEAERLYRVLMNC
                     DPTDAAAPFNLGNLFRATGRKIEAEAALRAATRADPMFAEAWYNLSDLLDEQGRSEAA
                     IDCLRRALQAAPGYADAMFNLALLLQRDSRHKEAAECWRQYLANDAQSEWAARARRSL
                     KFCEMQIHLSAC"
     gene            complement(1882..2172)
                     /locus_tag="VNY06_00495"
     CDS             complement(1882..2172)
                     /locus_tag="VNY06_00495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX13353.1"
                     /translation="MKRIINGFEVDWASERVFAEQQTAPNYGYLFEVSADRKRIVRCA
                     HISAIGGDSDNLDGDAERLEKDARQAVEEFLGCQRESGGLLGGWWRRRQKSA"
     gene            complement(3204..>3789)
                     /locus_tag="VNY06_00500"
     CDS             complement(3204..>3789)
                     /locus_tag="VNY06_00500"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWX13354.1"
                     /translation="TSVIPIVFAVAGDPVNTGLVASLAHPGGNVTGLSVQQTELAGKR
                     FELLREVVPGLRRLAIIGNPDNPSVVLEMDGAQAAARKLGLEVTRTEIRRAEDIAPAF
                     AALKGRAEALYVCNDPLVNTNRTRINILAVGARLLTVYNWRENVEAGGLMSYGANFLD
                     AFRRTAELIDKVLRGVKPADIPVEQRRSSISSST"
BASE COUNT          825 a         1188 c         1044 g          732 t
ORIGIN      
        1 ccacgagaat ccccctgatt caattggggc ggaacatgct ctagctggtt catgtcagcg
       61 ccagtggttc tcggctaggc cgatagcggc gactccaccg agcgcaaaaa ggaccgaacc
      121 caagataacg taaccccaaa tccaggcgac gcctgtgcag atgaatgaca gtgcggcgag
      181 caaagcaagg tcgcgcgtct tggcgtccat tcccgctccc gattagagag gggcagcgac
      241 taggctgccc cctaagcgcg agtcgatggc cttgaaccgc gaatgaaatg tctccccccg
      301 cgccgcagga cggcaggcca tctttctggg agcgaccgag ccacgcgctg cccgactggg
      361 aaaaacccca ccgcgcggga gtgacgctag taggccaagg ccggttggcg gtgtcaacgg
      421 ttggtaaaat acgctgacat tgacgcgcgc tgacgccgcg caaacaatcc caacatgacg
      481 attgtcaaga ttaccggcgg catgttgcgc gcagcgcgca gtctcgcctg gcctcacaag
      541 atagaaacgg taggaatcag atgatcgcgg cagtcgaagg gtccatgagt accgtctggc
      601 tttgatctta tgtttttgtt gtaatagctg cccatcgatg gtgctgccaa aaactccatc
      661 attgtctcgg gcttcacact gcaatggtga taatacgtgt ctttcagctt gataatcgta
      721 ttggcttcca gatagcacac ccgatttatg aaactgttgc gcgttataat ctcgcatttg
      781 aattgatcga tattgaggca cttgcggtat ttgacgcaca cctcctctgc ccccgcggga
      841 gacgatagca aaattgctac gatcatcccc gtaccggcga gcgtcgcccc tatcatcaag
      901 attcgaatta gaggctcctt tctccaagtg tcgcaatcac acatcttgca aacccgccgc
      961 caactcttgg cgaatgccgc catcgcgtcc tagcgcgtcg ccgcatagtc ccccgagcac
     1021 gaaaaagaca agacccaaaa cgaggtgatc ggaaatcaag gcgacacctg cgcagaagag
     1081 cgacagcacg gcgagcgaag caaggtctcg cgtcgtcttg gcatcgtttc cccggtccac
     1141 ggaagcgccg cccggcagct tctcagcacg ctgacagatg aatttgcatt tcgcagaatt
     1201 tcaaggatcg acgtgcgcgt gctgcccatt cagattgagc atcgttggca agatattgcc
     1261 gccagcactc cgccgcttcc ttgtgccgac tatctcgctg taacaaaagc gcaagattaa
     1321 acatcgcgtc ggcgtagccc ggggcagcct ggagcgcgcg ccgcagacaa tcgattgcgg
     1381 cttctgagcg accttgctcg tctagtaggt cggaaaggtt gtaccatgcc tccgcaaaca
     1441 tcggatcagc gcgcgtggcc gcacgcagcg ccgcttcggc ttcaatcttg cggccggtag
     1501 cccgaaacaa gttcccaagg ttgaacggcg ccgccgcatc tgtgggatcg caattcatca
     1561 agacacgata cagccgctcg gcttcggcaa tgtcgccggc ggcctctgct gcctgtgctt
     1621 gctcaaataa agcatcggca ttgggggccg gcgcgttgac cggcagcacc ccatccttca
     1681 ttgtgagaat ggcttcgcgg ccggcgatct tccactcacc gtgccgtcac gcgggaaacc
     1741 gttgccgacc tcgacggtac caaggatgcc gctcgccgag cggagcataa ccgatgcgta
     1801 gtcttcaacc gggcgctcga tgcgcgccgg ctcagttggg cgccagtcac ctgcgcctcc
     1861 tcgccggtca ggtgaaggtg ctcaggcgct cttctgccgc cgtcgccacc agccgccgag
     1921 gagaccaccg ctctcgcgct ggcagcctag gaactcctca acagcctgcc ttgcgtcttt
     1981 ttcaaggcgc tccgcgtccc catcgaggtt gtcggagtct cctccaattg ctgaaatgtg
     2041 agcacacctt acaatccgtt tccgatcggc ggacacctca aacaaatatc catagttcgg
     2101 cgcggtttgc tgctccgcga acacgcgctc gcttgcccaa tcaacctcaa agccattgat
     2161 gatgcgcttc ataatggctg ctccagatga ggcgctatca cctcaaccgc aaacatgcgg
     2221 tagctcaagc cgtctgatta gggaatggta tcccggatga tggcctggaa ggcggggctg
     2281 ccgcccgccc cgtcaggacc tcgcgctcaa cctctcagct aagcggcgtc tctgtaacac
     2341 agtaggaaag cgcttcattg acggcaccgc acgttgctgt gccgtctcta ccgcgtgctc
     2401 tctcgccgcg actacgacca atttggcgcg ctcgagcctc gccttttggt ctttgcggcg
     2461 gattcttggc ggagcaaagg cctccgtgct cgtcaacctc ttcacgatct gccccccgat
     2521 acggccggtg gctagccgac cacgaccaaa ttctgcccct ggatgacggc catctcatgg
     2581 ctctagggac ccagttccgg aaacaggtcc caaacgaaga aaggcgagac acagattcga
     2641 ccgacccccc ctctccccgc cgccccccgg gggttgcacc ggacccaatt gctgaaaaag
     2701 aaaaaacaaa aaaagagaaa agaccgaaag attcctgtcc tttcctcttt cggttggggg
     2761 aagcaatgtg ggtagacaga gctaaaataa ccaaacgatt tcaatgcacg acggcggaca
     2821 tgctttccgc cttgctgtgc tcgaccatcg caactgccga acagcccaac aactcagtgc
     2881 ccaagtcaac tcacttgagc gagccatgtg aaatgagaac accaacgcaa tagagcgagc
     2941 tataaccggg gcacgagcag cgctgaggtc taaaaagcta atgcgctgcc gtgttaataa
     3001 ccatgcaaat accggccgcg ccgggtaggc ttgcgcccac gacatacgcg agatcgccgg
     3061 cagcacggca gcgcctgcgg ccagatgcaa gaagtagcgg cggggaagtt tcattttgaa
     3121 cctcccttca catcactcaa tcacctcgtc ggcgcgggca agcaggctat cgggcaccgt
     3181 gaggccgagc gccttggcgg tggtcaggtt gatgacgaga tcgaacttcg tcgctgctcg
     3241 accgggatgt cggctggctt gaccccgcgc agaactttgt cgatcaactc cgcagtgcgt
     3301 cgaaacgcat caaggaagtt tgccccataa gacattagac cacctgcttc aacgttctcc
     3361 cgccaattgt aaaccgtcag caatcgcgcg cccacggcca agatgttgat gcgagtccgg
     3421 ttggtgttca caagcgggtc gttacagaca tacagcgcct ccgcgcggcc cttgagcgcg
     3481 gcgaacgcag gtgcgatatc ctccgcccgc cggatttcgg ttctagtgac ctcaaggcca
     3541 agcttgcggg ccgctgcctg ggccccgtcc atttccagca caacggaggg attgtcggga
     3601 ttgcctatga tcgccaaccg gcggagaccg gggacaacct cgcgcaatag ttcaaaccgc
     3661 ttgccagcaa gctcggtctg ctgaaccgat aggccggtga cgttgccgcc cggatgcgcc
     3721 aagctcgcga ccaggccggt gttgactggg tcgcccgcca ccgcgaacac gatcgggatg
     3781 accgatgtc
//
LOCUS       DATMPU010000024         3010 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_636883,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000024 DATMPU010000000
VERSION     DATMPU010000024.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 3010)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3010)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3010
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_636883"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            510..2264
                     /locus_tag="VNY06_00505"
     CDS             510..2264
                     /locus_tag="VNY06_00505"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013165165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 3 large subunit"
                     /protein_id="HWX13355.1"
                     /translation="MTKLMAGAEMVIRALQDHGVTTLFGYPGGAVLPIYDALFHQDQI
                     KHILVRHEQGAAHAAEGFARSSGKPGVLLVTSGPGATNAVTGLTDALMDSIPLVCITG
                     QVPTHLIGSDAFQECDTVGITRHCTKHNYLVRQIEDLPRILHEAFYVAQHGRPGPVVV
                     DIPKDVQFAIGTYIGPENIEHKTYRPRREGDPALIARAVAMMTAARRPVFYTGGGIIN
                     SGPEASRLLRELANLTGFPLTSTLMGLGAYPASGKHWIGMLGMHGTFEANNAMHGCDL
                     LIAIGARFDDRITGRLDAFSPGSKKIHIDIDPSSINKNVKVDLGIVGDCTHVLRQLIE
                     AWRGHSTDPATLAKWRAQIEKWRERKSLTFKNSTEVIKPQFAVQRLYELTKGRDTYIT
                     TEVGQHQMWAAQHYHFQEPNRLMTSGGLGTMGYGLPAAIGAQLAHPGSLVVDIAGEAS
                     ILMCIQELSTAAQYRLPVKIFILNNEYMGMVRQWQELLHDGRYSESYTVALPDFVRLA
                     EAFGAHGIRCSNPGRLDQAIIEMIDTPKAVVFDCIVDQTENCLPMIPSGKAHNEMILP
                     GETEEIVSAINASGKVLV"
     gene            2269..2820
                     /gene="ilvN"
                     /locus_tag="VNY06_00510"
     CDS             2269..2820
                     /gene="ilvN"
                     /locus_tag="VNY06_00510"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018406636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HWX13356.1"
                     /translation="MNAPAAPLSPYSSPLGKANSEVHTLSVLVDNEPGVLARVVGLFS
                     GRGYNIESLTVAEVAHAEHLSRITIVTSGPPEAIEQIGHQLERLVPIHKVTDLTLRGN
                     AVERELAMIKVAGTGQDRDEALRLADAFRARVIDASIESFVFEITGAVGKIDQFIELM
                     RPIGLVEVSRTGVAAMSRGPNPT"
BASE COUNT          619 a          865 c          889 g          637 t
ORIGIN      
        1 tcgctgaaat catgtcggaa cgctcaaggc ctgccgtggc gatccggtcg ccgagcagta
       61 gggcgcgggc cctcactctt gtcgtttttg gggaagcggg aagcgcaggg tgggtgttgg
      121 tcatggccca tgactatcga gcaggaaggg catagaaaag cgccaattca ggaaggcggt
      181 cgctgccaaa gcggtaaagg ctgcgcgctt acaatcaaaa aggcccgcga tcccgcccgg
      241 cacaaaccat attgaccata gttcggctat ccgctaggta taacagacgt atgggtaagg
      301 gtcttgattt cgagggtcta cgtgtgaaga ctattcttag cgtagcgcga gcgccggagg
      361 cgggacggag ataggctccg acttcccgct tcatcggcgc atgtccaggg cccatctggg
      421 ccttttttat tgcctggaag tagaaattga tctgcccgga caaacgcaga aagacggtag
      481 acattgaaac tcatcgcttg gagcaagtca tgactaaact gatggctgga gccgaaatgg
      541 tcatccgggc gctgcaggac catggcgtga ccacgctatt tggctatcca gggggagcgg
      601 ttctgccaat ttacgatgcg ctctttcatc aagatcagat caagcacatt ctcgtccgcc
      661 acgagcaagg tgcggcccat gcggcggaag gctttgcccg ctcgagtggc aagccggggg
      721 tgttgttggt tacctcagga cctggtgcca ccaatgccgt caccggcttg accgacgctt
      781 tgatggattc catcccgctt gtctgcatca ccggccaggt gccgacccat ctcatcggct
      841 ccgacgcgtt tcaggaatgc gacaccgtcg gcatcacgcg ccattgcacc aagcacaatt
      901 acctcgtccg ccagatcgag gatttgccgc gtattctgca tgaggctttt tatgtggccc
      961 agcacggccg gcctggtcca gtcgtcgtcg atattccgaa ggatgtgcaa ttcgcaatcg
     1021 ggacctacat cgggccggag aatatcgagc ataaaaccta ccggccaagg cgcgaggggg
     1081 atccggcctt gatcgcgcgg gcggtggcca tgatgacggc ggccaggcgc ccagtctttt
     1141 ataccggcgg cggcatcatc aattcgggtc ccgaggcttc gcgcctgctg cgcgagcttg
     1201 ccaatctcac gggatttccg ctcacctcaa ccttgatggg gcttggtgcc tatccggcct
     1261 ctggcaagca ttggattggc atgctcggca tgcatggcac ctttgaggca aataatgcga
     1321 tgcatggctg cgatttgctg atcgcaatcg gcgcgcgctt cgacgaccgg atcaccggga
     1381 ggctcgacgc tttttccccg ggctcgaaaa aaatccatat cgacatcgat ccctcctcga
     1441 tcaacaaaaa cgtcaaggtc gatctcggca tcgtcggtga ttgcacccat gtgcttcgtc
     1501 agctgatcga ggcttggcgc ggacattcga ctgaccccgc cacgctcgcc aaatggcggg
     1561 cgcagatcga aaaatggcgg gagcgcaagt cgcttacctt caaaaactca acggaagtaa
     1621 tcaaaccgca atttgcggtt cagcgtctgt atgagctgac caaagggcgc gatacttata
     1681 tcacgaccga agtcggccag caccaaatgt gggcggcgca gcactatcat ttccaggagc
     1741 cgaaccggct catgacctct ggcggccttg gcacgatggg gtatgggctt cccgcggcga
     1801 tcggcgcaca gctcgcccat cccggctcgc tcgtcgtcga catcgcgggc gaggcttcga
     1861 ttctcatgtg tatccaggag ctctcgacgg ccgcgcaata ccggctccca gtcaagattt
     1921 tcatcctcaa caacgaatat atgggcatgg tgcggcaatg gcaggagctt ttgcatgacg
     1981 gccgttattc ggaaagctac acagttgcgc tgcccgattt tgttaggctc gccgaagcct
     2041 ttggggcgca tggaattcgc tgttctaatc ctggccgact cgaccaagcc atcatcgaga
     2101 tgatcgacac gccgaaggcg gtggtctttg attgcatcgt tgaccagacg gaaaattgct
     2161 tgccgatgat tccctccggc aaggcgcata atgagatgat tctgcccggc gagactgaag
     2221 agattgttag cgcaatcaat gcctctggca aggtgctggt gtagtgacat gaatgctccg
     2281 gcggctcctc tctcgcccta ttcctccccg ctcggcaagg cgaatagcga ggtccacacg
     2341 ctctccgtgt tggttgacaa tgagccgggt gttcttgcgc gcgtcgtagg cctcttttct
     2401 ggacgcggct acaatattga aagtctgacg gtagcggagg tcgctcacgc cgagcatctc
     2461 tcgcgcatta ccattgtcac ctcagggccg ccggaggcaa tcgagcagat cggccatcag
     2521 ctggagcgcc ttgtgccgat ccataaggtc accgatttga ctcttcgtgg caatgcggtt
     2581 gagcgtgagc ttgcgatgat caaggtcgcg gggacgggac aagaccgcga tgaagcactg
     2641 cggcttgccg atgccttccg tgccagagtc atcgacgctt cgatcgaaag ctttgtgttc
     2701 gagatcaccg gcgccgttgg caagatcgac caattcatcg agctcatgcg gccgatcggc
     2761 ctcgtcgaag tctcgcgcac cggagtcgcc gcaatgtcgc gcggaccaaa tccgacgtag
     2821 cttcttgaaa gccaactgtt cggagatgca tgacaccgtt tgccgccgca gttcttgcgc
     2881 gtagcgtcgc gcttagccct gattgcggga gacatcggcg agggtagcgt cgacagagcg
     2941 ggaacggccg ccgcggacga ccgtcaacgt caccgttttg tcggcaccaa cctgttcgaa
     3001 tatgctcgca
//
LOCUS       DATMPU010000025         7013 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_3737997,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000025 DATMPU010000000
VERSION     DATMPU010000025.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 7013)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7013)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7013
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3737997"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            167..2173
                     /locus_tag="VNY06_00515"
     CDS             167..2173
                     /locus_tag="VNY06_00515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011088023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl/propionyl/methylcrotonyl-CoA carboxylase
                     subunit alpha"
                     /protein_id="HWX13357.1"
                     /translation="MFKKILVANRGEIACRIIATARRMGIAVVAVYSDADRDALHVEM
                     ADEAMPIGPPPIAQSYLVIERIVAACKETGVEAVHPGYGFLSEQAGFARVLEDNNIAF
                     IGPNPKAIAAMGDKVIAKEIAGRAGIKVVPGVADVIGDVTEAAKAAEAIGYPVMIKAS
                     AGGGGRGMRVVAEREGLAEGFARAESEAKATFGDGRVFIEKLIAAPRHIEVQLLADKH
                     GNIIHLGERECSIQRRHQKLIEEAPSSALDEEGRSKLTAQAVALAKAVNYDSAGTVEF
                     IAGEDKSFYFLEMNTRLQVEHPVTELATGIDLVEQMIRSAAGEKLSIIQDEVRRKGWA
                     VESRILAEDPERNFLPSGGRLKTYRPPAAGVQDGRTLRIDSGVREGSEISIHYDPLIA
                     KIVTHTADRAAAIKSQAQALDSFVVEGPRTNVAFLSAVMRHKRFLAGELSTGFIAEEY
                     PRGFAPVIPDGKIARLMASVAAAADHVAHQRKRAISGQMDKATPVPTAAKRSVLLGNA
                     RFDVEIESRGDGVAVRFEVHGERHLCISGWKPGEPLWEGTVDGECLAVKLRPALNGHV
                     LTHGGAITEARVYTRREAELTAFLPKKKAASGLNVLRSPMPALVKSIEVVAGQEVKTG
                     EPLCIIEAMKMETMLRAEQDGTVKAVHVKPGASIAVDAVIMAFG"
     gene            complement(2306..3673)
                     /locus_tag="VNY06_00520"
     CDS             complement(2306..3673)
                     /locus_tag="VNY06_00520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012589573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HWX13358.1"
                     /translation="MASDILIVDDEADIREIVSGILADEGHGTRAAKNSDEALAAIGA
                     RRPTLVFLDIWLQGSRLDGLQLLKIIKEQNPMLPVVMISGHGNIETAVSAIKLGAYDF
                     IEKPFKADRLVLVAERALEASRLKREVTELRSRAGGDNQIVGKSTAMNQLRQVIERVG
                     PANSRVLITGAPGAGKELVARCIHDASQRASGPFVTLNSATIAPETMEMELFGAEASE
                     GRSRKVGALEEAHGGTLYLDEIADMPKETQSKILRVLVEQNFLRVGGTTRVFVDVRIL
                     SSSSRDLAALIENGSFREDLFHRLAVVPVRVPSLSEHREDIPELVGFFMEQLSITTGM
                     PRRLIAEDAMAVLQTHDWPGNIRQLRNNVERLMILAAGDPEAALTASMLPTEIGALVP
                     STPNGQGGEKLMNLPLREAREVFEREYLFAQINRFGGNISRTAEFVGMERSALHRKLK
                     SLQVD"
     gene            complement(3682..5952)
                     /locus_tag="VNY06_00525"
     CDS             complement(3682..5952)
                     /locus_tag="VNY06_00525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012589572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HWX13359.1"
                     /translation="MGDRKTLRQSLGSKKDPPRFTARFGPVAVIFALFIGLISFLIFS
                     GFTPVAPTDGVVLGLFLANVLCILLVLSFIAAEAYALFKARRAGVAGAELHVRIVGLF
                     SIIAAAPALLMAWVGSVTLERSLNPSFMQDGRGFVQNTIEAAHLFQEGQCRSLLQEAR
                     LTSTDLDRAKLMFEVDRPLFREFFTTRARYLGFTAAALLKSDGTVLERVDTGAVIGNT
                     VVRPESSDFEDARKNEATCLILDEGRTFVALRALLSFQDTFLYAARPVDPFTVEFPRQ
                     ASRLVALYDAFDEHRRGIRIAFATMFVLIALIMLLSATWLGLSFADSLVAPIRRLIAA
                     TDDVASGNLHVQVPVRKSEGDLAHLGETFNKMTSELSLQQRRLIAASKLIDERRLFTE
                     AVLSGVPVAVIGVGPKGEITALNPSAEKLLPQKEETAARLTGQPIATVMPELDGMLAD
                     ARSGQSRLVQGQLSLSRSGLDRLFNVSITSEPSARADKSYVVTLEDITDLVTAQRTAA
                     WADVARRIAHEIKNPLTPIQLSAERLKRKYGRVIEHDRDIFDQCTDTIVRQVDDIKRM
                     VDEFSAFARMPRARLESEDLGECVRQVLFLMRVGHSDISFEDHLPKEPIHVPFDRRLL
                     AQALTNIVKNATEGIAERSSEEGFKGKISVTVTVVGNDVSIDVIDNGKGFPKENRQRL
                     LEPYMTTRADGTGLGLPIVAKILADHEGGLELRDAPSGRGAWVRLFFPLGRRSVAEAQ
                     GGDADAAIAARAKTGG"
     gene            complement(6342..>7013)
                     /locus_tag="VNY06_00530"
     CDS             complement(6342..>7013)
                     /locus_tag="VNY06_00530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012589571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HWX13360.1"
                     /translation="KTNVRIIAATNKELGGLIRQGLFREDLFFRLNVVPLRLPPLRER
                     LEDVGDLAHHFFALAAAEGLPLKRIEPDALERLKRHRWPGNIRELENLTRRLAALYPQ
                     EVVTTQLADLELSVKQPMIIPTSPTVPGIDDSQSSESQRQLTLASAMEHHLSELFQER
                     GGELPPPGLYHRILREIEHPLITAALAATRGNQIKAAELLGLNRNTLRKKVRDLDIRW
                     VRAPR"
BASE COUNT         1518 a         1981 c         2077 g         1437 t
ORIGIN      
        1 accgcacgga tgcaatgccg aggcgcatcc tctttatggg gggctacgtt caacgtagct
       61 cagggctcgc caacgcatac agccgacagt gtcccatgtt atttagcctc tggtctcatt
      121 gtttgttctg gacacggctc ctttgcgcgc ctttacggga tcccaaatgt tcaaaaaaat
      181 cctcgtagcg aaccgcggcg agattgcctg ccgcatcatt gcgacagcgc ggcgaatggg
      241 gattgctgtg gttgccgtct attcggatgc agatagggat gcgctccatg tggagatggc
      301 ggacgaggcc atgccgattg ggccgcctcc tatcgcgcaa agctatctcg tgatcgagcg
      361 cattgtcgcc gcctgcaagg aaacgggcgt ggaagcagtg catcctggtt acggattttt
      421 gtcggaacag gcaggtttcg cgcgcgtatt ggaggacaac aacattgcat ttattggtcc
      481 gaacccgaag gccatcgcag caatgggcga caaggtaata gcgaaggaaa tagcgggaag
      541 ggcgggcatc aaggtcgtgc cgggtgttgc ggacgtgatc ggcgacgtca ctgaagcggc
      601 aaaggccgcc gaagccatcg gctatccggt aatgatcaaa gcctcggcag gtggcggcgg
      661 cagagggatg cgcgtcgtcg ctgaaagaga agggctcgcc gaaggtttcg cccgggcgga
      721 atcggaagca aaagccacct ttggcgacgg cagggttttt atcgaaaaat taattgccgc
      781 tccgaggcac atcgaggttc agctgctcgc cgacaaacat ggaaatatca tccatcttgg
      841 cgaacgcgaa tgttccatcc agcgccgcca ccagaagctg atcgaggaag cgccttcttc
      901 cgccctcgat gaggaaggcc gaagcaagct caccgcgcaa gcggtcgctc ttgctaaggc
      961 tgtcaactac gattcggccg ggacagtcga atttatcgcg ggcgaagaca aaagcttcta
     1021 ttttctcgaa atgaacacac gcctgcaggt cgaacacccg gtgaccgagc ttgccaccgg
     1081 cattgatctc gtcgaacaga tgatccggtc agccgcagga gagaaactat cgatcattca
     1141 agacgaggtg cgccggaaag gctgggcggt tgaatcccgc attcttgccg aggacccgga
     1201 acggaatttt ctcccgtccg gcggccgtct caagacctac cgccccccgg ctgccggggt
     1261 acaggacggc agaacgctgc ggatcgattc cggtgttcgc gaaggcagtg agatctccat
     1321 tcattatgat ccactgatcg ccaaaatcgt gacccatacg gcggaccgcg cggcagccat
     1381 aaagtcccag gcgcaggccc tcgattcctt tgtcgtcgag gggccaagga cgaatgtcgc
     1441 ttttttatcg gcggtaatga ggcataagcg gttccttgct ggcgagcttt cgacgggctt
     1501 tatcgctgaa gaataccctc ggggcttcgc tcctgttatt ccggacggga aaatcgcgcg
     1561 attgatggca agcgttgcag ccgctgcaga tcatgtggcg caccaacgca agcgcgcgat
     1621 ctcaggccag atggacaaag caactccagt cccgactgcg gcgaagcgtt cggtgctgct
     1681 tggcaacgcg cgttttgacg tggaaattga gtcgcgcggc gatggcgttg ccgtgcgctt
     1741 tgaggtccat ggtgaaaggc atctctgcat ttccggttgg aagccaggtg agccgctttg
     1801 ggaaggcacg gttgacggcg aatgtctcgc agtaaaattg cgtcccgcac tcaacggcca
     1861 tgttttgacg catggcggcg ccatcaccga agcacgcgtc tacacacgcc gcgaggcgga
     1921 gcttaccgcg ttcctgccaa agaaaaaggc agcgtctggc ctcaacgtgc tgcgctctcc
     1981 catgccggcc ctcgtcaaat caatcgaagt tgttgccgga caggaggtca aaacaggtga
     2041 accactttgc atcatcgaag cgatgaaaat ggaaacgatg ctgcgcgccg aacaggatgg
     2101 gaccgtgaaa gcggtacatg taaagccggg agcctctatc gccgtcgatg ccgttatcat
     2161 ggcgtttggg tgagcgtggg ttcaaatttt ccagcgttcg ccgctggcgg aaaaggccgc
     2221 agaaaggttt tggatttctg ctattgcgca gccgctggct cataaccaat cggctcttaa
     2281 gcaacaaatt tcaaagcaac aaatttcagt cgacctgaag cgatttcagc ttgcggtgaa
     2341 gcgccgagcg ttccatgcca acgaattctg ccgtgcgcga aatattgccg ccgaaccggt
     2401 tgatttgggc aaacagatat tcccgctcaa agacttcccg ggcttcccgc aatggcaaat
     2461 tcatcagctt ttcgccgcct tggccatttg gcgtcgaagg cacgagcgcg ccgatttccg
     2521 tgggcagcat ggaagcggtg agcgccgcct cgggatcgcc agcggcaagt atcatcaacc
     2581 gctccacatt gttgcgcaat tgccggatgt tgcctggcca atcatgcgtt tgcaaaaccg
     2641 ccattgcgtc ctcagcaatc aatcgccgtg gcatgccggt tgttatcgag agctgctcca
     2701 tgaagaaacc cacgagttcg gggatatctt cgcggtgctc gctaagggaa ggaacgcgaa
     2761 ctggaacgac agcaagacga tgaaagagat cttcgcgaaa cgacccgttc tcaatcaaag
     2821 cggcaagatc gcgcgagctt gacgagagaa tgcgcacgtc cacaaaaacc cgcgtcgtgc
     2881 cgccgacacg aagaaaattc tgctcgacaa gcacccgcaa aatcttgctc tgggtttcct
     2941 tgggcatatc ggcgatttca tcgagataaa gcgtgccgcc atgggcttcc tcgagcgccc
     3001 cgaccttgcg gcttctgcct tcggaggctt cggccccaaa gagttccatt tccatggttt
     3061 cgggcgcgat cgtcgccgag ttgagggtga caaagggccc gcttgcacgc tgggaggcat
     3121 catgaatgca gcgggcgacg agctccttgc cggcaccggg tgcgccggtg atcagaacgc
     3181 gggaattggc cggtcctacg cgctcaatca cttggcgcaa ttgattcatc gctgtcgatt
     3241 tgccgacgat ctgattgtcg ccaccggccc tgctgcgcaa ctcggtgact tcgcgcttca
     3301 gtcgcgaggc ttcgagcgcg cgttccgcca ccagcacaag gcgatccgcc ttgaatggct
     3361 tttcgatgaa atcataggcg ccgagtttta tcgcagaaac cgccgtctca atattgccgt
     3421 gaccggagat catgacgacc ggcagcattg ggttctgttc cttgatgatc tttaggagct
     3481 gcaagccgtc gagccgcgag ccctgcagcc aaatatcgag aaacaccagg gtcggccttc
     3541 tcgccccgat cgcggccaaa gcctcgtcgg aatttttggc tgcacgcgtg ccatggcctt
     3601 catccgccaa gatcccggag acgatttcac gaatgtcggc ttcgtcatca actattagga
     3661 tatcgcttgc catgactcct cctaacctcc cgtcttggct ctagccgcta tggctgcatc
     3721 cgcgtccccg ccctgagcct ccgcgactga gcggcgccct aaggggaaaa acagcctgac
     3781 ccaggcacct cggccgctcg gtgcgtcgcg cagttcaagt cctccttcat ggtctgcgag
     3841 aattttggcg acgatcggaa gaccgagccc cgtgccatcc gcgcgcgtcg tcatgtacgg
     3901 ttcgagcagc cgctgccggt tttcttttgg aaaccccttg ccattgtcaa tcacgtcgat
     3961 ggaaacgtca ttgccgacga cggtgaccgt cacggatatt ttccccttaa atccttcttc
     4021 gctggagcgt tcggcaatcc cttcggttgc gttctttaca atattggtca aggcttgggc
     4081 caaaagccgc cggtcgaagg gaacgtgaat aggctccttg ggcaggtggt cctcaaacga
     4141 aatatcggaa tggccgacgc gcatcaaaaa cagaacttgg cgcacgcatt caccaaggtc
     4201 ttcgctttcg aggcgcgctc tcggcatccg cgcgaaggcc gaaaattcgt ccaccatgcg
     4261 cttgatgtca tcgacttgac gaacgattgt atcggtgcat tggtcgaaaa tatcccggtc
     4321 atgctcgatc acgcgtccgt atttgcgttt caaccgttcg gccgagagct gaattggggt
     4381 tagtggattt ttgatttcgt gtgcaatgcg gcgagcgaca tcggcccaag cggccgtccg
     4441 ctgcgcggtc acgagatcgg tgatgtcctc aagagtgacg acatagctct tgtctgcgcg
     4501 tgcactcggt tcgctggtaa tgctgacatt aaagagacga tcgaggccgc tgcgggaaag
     4561 tgagagttgg ccctgaacaa ggcgcgattg acccgaccgc gcgtcggcga gcatcccatc
     4621 aagttccggc atcaccgtcg ctatcggctg gcccgtgagt cgcgccgcgg tttcctcttt
     4681 ctgtggaagc aatttttcgg cggagggatt gagtgccgtg atttcgcctt tcgggccgac
     4741 gccaatgacc gcgaccggaa caccggagag aacggcctcg gtaaacaggc gccgctcgtc
     4801 gatgagcttg cttgccgcga tcaggcggcg ctgctggagg cttagctccg aggtcatttt
     4861 gttgaaggtt tcgccgagat gggcgagatc gccttcggat ttgcgcacag gcacttggac
     4921 atggagattg ccggaggcaa catcatcggt tgccgcgatg aggcgccgaa tcggggcgac
     4981 aagactgtcc gcaaaagaaa gaccgagcca ggtcgcagac aagagcatga tgagagcgat
     5041 caggacgaac atcgttgcga aagcgatccg gatcccgcgc cggtgctcgt cgaaagcgtc
     5101 ataaagcgca acgagcctcg acgcttgacg cggaaactcc acggtaaaag gatcgaccgg
     5161 ccgggcagcg tataaaaatg tatcttggaa cgagagaagc gcgcgcaaag cgacaaaggt
     5221 ccgcccctca tccaagatca aacatgtggc ctcgtttttt cttgcatcct caaagtcgct
     5281 cgactccgga cgcaccacgg tgttgccaat caccgcgccc gtatccaccc gctcgaggac
     5341 tgtgccatcg gattttagga gcgctgcggc ggtaaaaccg agataccttg cccttgtcgt
     5401 gaaaaactct cggaacaacg gccggtccac ctcgaacatg agcttggcgc ggtcaagatc
     5461 ggtcgaggtg agccttgctt cttggaggag cgaccggcac tggccctctt gaaacaggtg
     5521 cgcagcttcg attgtgtttt gcacgaagcc gcgtccgtcc tgcatgaagg acggattgag
     5581 gcttcgctcc aacgtcaccg aaccgaccca agccatcagc agcgccggcg cggcggcaat
     5641 gatcgaaaaa agtccgacga tcctgacgtg cagctctgcg ccggcgacac ccgcccggcg
     5701 cgctttgaaa agcgcgtagg cctcggcggc aataaaactc aagacgagga ggatgcagag
     5761 gacattggcg agaaatagtc cgagaacgac gccatcggtg ggagcgaccg gcgtgaagcc
     5821 cgaaaaaatc agaaagctga tgaggccaat gaagagggca aaaataaccg ctacgggacc
     5881 aaaacgggcc gtaaaccggg gtgggtcctt cttgctgccc aagctctgcc gcaatgtttt
     5941 tcgatcgccc atagccgcgg actagaatcc atggaaggat taggttgcat caaatatcga
     6001 gacgcgcgtt gcgatattat cacgctgttg ccagattgcg acatttccgg ggccagcgcc
     6061 agcaattgac ataaagatcc gacgaagcaa tcgcacagag gcaggcatgg gattcgacaa
     6121 cggcagtcgc ggacgcgcca agcatcaaat atttaaggct tttctcttta acgatttggt
     6181 ctttaacgga tttggatgtg gcgtcttctt gcgcggtcga gtcaccatgg ggtcggccgc
     6241 aggcggtcgc ggccagcagc ccgccgtgcg taccagcgca gacagccgtc aattcttgag
     6301 ccgagcagtt ccggaccaga cagtcgtgca gtgccggtgc atcagcgcgg tgcacgcacc
     6361 catctaatgt cgagatcgcg cacctttttg cgcaatgtgt tccggttgag ccccaaaagt
     6421 tcggcggcct taatctggtt gcctcgcgtt gcagcaagcg ccgcagtaat caatggatgc
     6481 tcgatctccc gcaggattct gtgatagagg ccgggcggcg gtaattctcc gccgcgttcc
     6541 tgaaacagct cggagaggtg gtgttccata gcgcttgcga gcgtcagctg cctttgagac
     6601 tcgcttgact gcgaatcgtc gataccgggc accgttgggg aagttggaat tatcatcggc
     6661 tgtttgacgc taagttccag atccgccaat tgcgttgtga cgacttcttg gggataaagc
     6721 gcggcgaggc gacgcgtcaa attttcgagt tcccggatat tgcctggcca gcgatgccgc
     6781 ttcaggcgct ccagggcgtc cggttcgatc cgcttcagcg gcaagccttc ggcggcggca
     6841 agggcgaaaa aatgatgcgc gaggtcgcca acatcctcga ggcgctcgcg tagcggggga
     6901 agccgcaaag gcaccacgtt taaccggaag aacagatctt cgcggaaaag gccttggcgg
     6961 atcaggccgc cgagttcctt gttcgtcgcg gcgataatgc ggacatttgt ctt
//
LOCUS       DATMPU010000026         5055 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_3118614,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000026 DATMPU010000000
VERSION     DATMPU010000026.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 5055)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5055)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5055
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3118614"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            76..1317
                     /locus_tag="VNY06_00535"
     CDS             76..1317
                     /locus_tag="VNY06_00535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015933415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HWX13361.1"
                     /translation="MAGRHFLFVPGPTNVPDRVTRAMMVAMEDHRSSKFPELTLPLFR
                     DLKKVFKTAEGQVFIFPSAGTGAWEASLCNTLSPGDKVLASRFGQFSHLWIDLAQRLG
                     LDVQILEEEWGTGVKPDKVESVLRADKSHQIKAVLAVQNETATGVTSDIEAVRKAMDA
                     AGHPAMLYVDGVSSIASIEFQMDEWRVDLAVAGSQKGLMLPAGLGFVCASPRALAAYK
                     TAELKRVYFDFGDMMKANATGYFPYTPALPMLYGLREALIMLLEEEKLQHVFERHHVL
                     ASGVRAAVKAWGLQLCATEPQWYSDTVTAIVVPPGFNGADVIDAAFRRYNLSLGAGLS
                     QVAGKVFRIGHLGDLNDLMVLGAIAGAEMAMRDIGIEIQLGSGVAAAQAHFRSTIMAK
                     EKKPTDQEHFAQLHPEGVLND"
     gene            1579..2520
                     /locus_tag="VNY06_00540"
     CDS             1579..2520
                     /locus_tag="VNY06_00540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003597638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HWX13362.1"
                     /translation="MQHSIVFLDRSTLEAKLRAPNFPHSYKEYAVTAVNEIVDRLRGT
                     TIAIINKVPMREATLAELPDLKLIAVAATGTDVVDKAFCKVRGITVSNIRNYAFNTVP
                     EHVFALAFALRRNLVAYRDDVRAGRWQACDQFCFFDHPIRDMRGSTIGIVGYGAIGKS
                     VAKIAEAFGLKVLAYDVIAQPGLTGFEILLRESDIITLHLPLTPETKNMIGARELRLM
                     KNDAVLINTGRGGLVDEQALADALTGKIIGGAGFDVLTVEPPKQGNILLDLRLPNFIV
                     TPHVAWASREAMQVLADQLVDNIDAFAAGKPQNVVEA"
     gene            2544..4220
                     /locus_tag="VNY06_00545"
     CDS             2544..4220
                     /locus_tag="VNY06_00545"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012605559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HWX13363.1"
                     /translation="MPADIEIAQNATMRRISDVARDKLGITDEHLEPFGYYKAKIALG
                     YIDSLADRPDGKLILVTAISPTPAGEGKTTTTVGLGDALNRIGKKASICLREPSLGPV
                     FGMKGGAAGGGYAQVVPMEDINLHFTGDFGAIGLAHNLLAAMIDNHIHHGNELDFDVR
                     RIAWKRVVDMNDRALRQTTIALGGTANGYPREDGFDIVVASEIMAIFCLATSLKDLKD
                     RLARIVVGYTRDQKPIHAGDLDAHGAMAVLLKNALKPNLVQTLENNPAFIHGGPFANI
                     AHGCNSVLATKTALKLSDYVVTEAGFGADLGAEKFIDIKCRKSGLRPQAVVIVATVRA
                     LKYHGGADLKDLNTENLDALAKGVANLERHVNNIRNHFGLPCVVAINHFTFDTDAEIE
                     LLKKKMAHHEAPIVVCRHWAEGGKGAEELARVVADMIDKVPVDFRFVYEDSASLWDKA
                     AAIATKLYGASKVTADSKVRTQIKRLQDSGYGHYPVCVAKTQYSFSTDAKLRGAPTHH
                     EINIREVRLAAGAEFIVLVCGDVMTMPGLPKVPAATKIDLDDNGKVTGLF"
     gene            complement(4217..>5055)
                     /locus_tag="VNY06_00550"
     CDS             complement(4217..>5055)
                     /locus_tag="VNY06_00550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020174135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HWX13364.1"
                     /translation="PLKALIFDVDGTLAETEEAHRQSFNEAFASFGLDWNWDKELYRT
                     LLQVTGGKERLQHYINESKPPGSAVAHSRFTEIYEEASARYAALVESGAVLARRGVRR
                     LIMEAHERGVRLAIATTSLQDSVLTLLQTILGKEGPAMFAAIAAGDAVANKKPAPDIY
                     FLALEMLRCGPDSCVSIEDSTNGIKAARAAGLPVIATPSYYLLNDDYTLATSVLSDLG
                     EPKSPCHRIAGPSFDRDYVDIDGLNAWLTKQQRSDALARAAPALTNKRAVDALGHRRN
                     RF"
BASE COUNT         1096 a         1399 c         1482 g         1078 t
ORIGIN      
        1 cgcgcggaac gcggttgcgc ttgcgccggt ggttttgtta gtaatgtaac ccggcaaaca
       61 aatggaggat ttcttatggc gggccgccac tttctttttg taccagggcc aaccaatgtc
      121 ccggaccgcg tcacccgcgc catgatggtg gcgatggaag accataggtc ttccaaattc
      181 cccgagctta cgcttccatt gtttcgggat ttgaagaaag tttttaagac cgccgagggg
      241 caggttttca ttttcccatc cgccggcacg ggtgcttggg aagcgtcgct ctgcaacacg
      301 ctttcccccg gtgacaaagt cttggcctcg cgattcggcc aattcagcca tttgtggatt
      361 gatttggccc agcgcctcgg cctcgacgtg cagattttgg aggaggaatg gggtacgggt
      421 gtcaagccgg acaaagttga atctgttttg cgtgccgata agagtcacca aatcaaagcc
      481 gtgttggcgg tccagaatga gaccgcgacc ggtgtgacga gtgatatcga agccgtgcga
      541 aaggcgatgg acgccgctgg tcacccggcg atgctctatg tcgatggcgt aagctcgatt
      601 gcaagcatcg aattccagat ggatgaatgg cgggttgatc ttgccgtcgc gggatcgcag
      661 aaaggtctta tgcttccggc cggtcttggc tttgtttgcg cgagcccccg cgctcttgcc
      721 gcctataaga cggccgagct gaagcgagtc tattttgact tcggcgacat gatgaaggcc
      781 aacgcgacgg gttattttcc ctacaccccg gcattgccga tgctctatgg cttgcgtgag
      841 gcgctcatca tgcttctcga agaggagaag cttcaacatg tgttcgaacg ccatcacgtg
      901 ctcgccagcg gtgtgcgcgc tgctgtcaag gcgtggggtt tgcagctatg cgcgacggaa
      961 ccgcaatggt attcggatac ggtcaccgca attgtcgtgc cgcctggctt caatggcgcc
     1021 gatgtgatcg acgccgcttt ccgccgctat aatttgtcgc tcggtgccgg cctatcacag
     1081 gtcgcgggca aggttttccg gatcggtcat cttggtgacc tcaacgacct tatggttctg
     1141 ggtgcaattg ccggcgcgga aatggcgatg cgcgatattg gcatagagat tcagctgggc
     1201 agcggcgtcg ccgcggccca ggcgcatttc cgctcaacca tcatggccaa ggaaaagaag
     1261 ccgacggatc aggagcattt cgcgcagctg cacccggagg gcgtcctcaa cgactgagac
     1321 ttatgtggtg ccggacgcgc gcgcattcgt cacgcgcgcg gggttccatg tcggccgcac
     1381 acattcgctc ttggagcggg tcctgaaagc ttgaatagat ggcggcccgg cctccgaggg
     1441 aaagccagcc gccgatgggg atcatccaat ctccgcggca acctgtcatc gacatcgatc
     1501 tcacggaatt acggcgcagt gaaaagccga ctgtctgcga agcctcaaga aaaactagat
     1561 tcaaggaaag agtacgctgt gcaacactcg atcgtctttc tcgaccgttc gaccttagag
     1621 gccaagctgc gtgcgccgaa ttttccccat agctacaagg aatacgcagt aaccgcggta
     1681 aatgagatcg tcgaccggct gcggggtacg acaattgcca tcatcaacaa agtgcccatg
     1741 cgcgaagcaa cgctcgctga gctccccgat cttaagctca ttgcagtcgc ggccacgggc
     1801 accgatgttg tcgacaaagc cttctgcaag gtgcgcggaa ttactgtgtc gaacataagg
     1861 aactacgcct ttaacaccgt gccggagcat gtcttcgccc tggcttttgc gcttcgccgc
     1921 aacctcgttg catatcggga cgatgtgcgt gccgggcgct ggcaggcctg cgatcaattc
     1981 tgcttctttg atcatcctat tcgcgacatg cgcggctcga cgatcggcat tgtcggctat
     2041 ggcgcaatcg gcaagtctgt agccaagatt gctgaagctt ttggcttgaa ggtgctggct
     2101 tatgacgtca tcgcgcagcc ggggctcacc ggctttgaaa tcttgctgcg cgagagcgac
     2161 atcatcactc tgcatctgcc gttgacgccg gaaaccaaga acatgattgg cgcgcgcgaa
     2221 ttgcggctca tgaaaaatga tgcggtccta atcaacacgg gccgcggcgg ccttgttgac
     2281 gagcaggcgc tggccgacgc gctgaccggc aagatcatcg gcggcgcggg ctttgacgtg
     2341 ctgaccgtcg agccgccaaa gcaaggaaac atcttgcttg atttgcggtt gccaaatttc
     2401 atcgtcacgc cgcatgtcgc ctgggccagc cgcgaggcta tgcaggttct cgccgaccaa
     2461 cttgtcgaca acatcgatgc attcgcggca ggaaagccgc aaaacgtggt cgaagcctga
     2521 atacggcttc gagaggagga accatgccag cggacatcga aatcgcgcaa aatgcaacga
     2581 tgcgccggat tagcgatgtg gcgcgggaca aacttggcat tacggatgag catcttgagc
     2641 cctttggcta ttacaaagcc aagattgcgc tgggttacat tgacagtctc gcagaccggc
     2701 cagatggcaa actcattctc gtcaccgcga tcagtcctac cccggctggg gaaggcaaga
     2761 ccacaacgac ggttggactt ggcgatgcgc tgaacaggat cggcaagaaa gcatctattt
     2821 gtttgcgcga gccatcgctt gggcccgtat tcggcatgaa aggcggggcc gccggtggcg
     2881 gctatgcgca ggttgtacca atggaggata tcaatctcca ttttactggc gacttcggcg
     2941 caatcggtct tgcccataat cttcttgcgg cgatgatcga caatcatatt caccatggaa
     3001 atgaacttga cttcgacgtg aggcgcatcg cctggaaacg cgtggtcgat atgaacgatc
     3061 gcgcccttag gcaaaccacc atcgccttgg ggggaacggc aaatggatat ccgcgcgaag
     3121 acggttttga tatcgtcgtc gcatccgaga tcatggcgat tttctgcctt gccacttcgc
     3181 tcaaggattt gaaagaccgg ctggcgcgga tcgtcgtagg gtatacgcga gaccagaagc
     3241 cgatccacgc gggcgatctc gatgcacatg gcgcaatggc ggtgctccta aagaatgcgc
     3301 ttaagccaaa tctcgtgcaa acgctcgaaa acaatccggc gtttattcac ggcggaccgt
     3361 ttgccaacat tgctcatggc tgcaattccg tgctcgctac caagactgcg ctgaaactct
     3421 ccgactatgt cgtgaccgag gcggggtttg gcgccgatct tggagccgaa aaattcatcg
     3481 acatcaaatg ccgcaaatcg gggctgcgtc cgcaggcggt ggtgattgtc gcaaccgtcc
     3541 gcgcgctcaa ataccacggg ggcgcagacc tcaaggatct gaacacggaa aacctcgatg
     3601 cactggcgaa gggcgttgcc aatttggaac gccacgtgaa caacatccgt aaccactttg
     3661 gactgccctg cgtcgttgcg attaaccatt tcacctttga cacagatgcc gaaatcgagc
     3721 ttttgaagaa aaagatggcg caccacgaag cgccaatcgt tgtgtgccgt cattgggccg
     3781 agggcggaaa aggcgccgaa gaattggcgc gggtcgtggc ggacatgatc gacaaggtgc
     3841 cggtcgactt ccgctttgtg tatgaggatt ccgcctcgct ttgggataag gcggcggcaa
     3901 ttgccaccaa actttatggc gcgagcaaag tcaccgcaga ttcaaaggtg cgcacgcaaa
     3961 tcaagcggct tcaggatagc ggctatggtc actatcctgt ttgcgtcgca aagacgcagt
     4021 attccttttc gacggatgcg aagctgcgtg gcgcgccgac tcatcatgag atcaacatcc
     4081 gcgaagtccg gcttgccgct ggagctgaat tcatcgtgct tgtgtgcggc gacgtgatga
     4141 ccatgccggg cctgcccaag gtgccggcgg caacgaaaat tgatttggac gataacggca
     4201 aggttacggg gctattttaa aaccggttcc gccgatggcc gagtgcatcc accgcgcgct
     4261 tgttggttag ggcgggagct gcccgagcca aagcatcgga cctctgttgc ttggtgagcc
     4321 acgcgttcaa gccatcaata tccacatagt cgcgatcaaa gctcggaccg gcaatccggt
     4381 ggcaaggact cttcggttct ccaaggtcgc ttaggacgct ggtcgcaaga gtgtaatcat
     4441 cgttaagcaa ataataactt ggcgtggcga tcacgggcaa gccggcagcc ctcgccgctt
     4501 tgatgccgtt ggtcgagtcc tcgattgaca cacaagaatc tgggccgcac cggagcattt
     4561 cgagagccaa aaaatagata tcgggcgcag gctttttatt tgcgacggca tcgccagcgg
     4621 caatcgccgc gaacatggca ggaccctctt tgcccaggat cgtttgcagc agggtcagga
     4681 cactgtcctg aagcgacgtc gttgcgatgg cgagccgcac gccccgttca tgagcctcca
     4741 tgatcaagcg gcgaactcca cgccgtgcta gaactgcacc tgattcgacc agcgccgcat
     4801 agcgggctga agcctcctcg taaatctccg tgaagcggga atgcgccaca gcactgcccg
     4861 gaggtttcga ttcgttgata taatgctgga gacgctcctt gccaccggtg acctggagaa
     4921 gcgtgcgata gagctccttg tcccagttcc aatcgagccc gaaggaagca aaagcctcgt
     4981 tgaaagattg tcgatgtgcc tcttcggttt cggcaagcgt cccgtccaca tcgaatatca
     5041 gagccttaag cggca
//
LOCUS       DATMPU010000027         8083 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_4358946,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000027 DATMPU010000000
VERSION     DATMPU010000027.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 8083)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8083)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8083
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4358946"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..380
                     /locus_tag="VNY06_00555"
     CDS             <1..380
                     /locus_tag="VNY06_00555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX13365.1"
                     /translation="RVFFRILNETPKRPRKDAVSETTRKRLEALNQYDIELYEWVKER
                     FAKQIEPLEPDFSREVRRFEMLNRSVRRIRQLTPRPIHRTMKQLLLSPQRLSQWFCAH
                     ALFAICDTAFFRLDYSTVLVASF"
     gene            1310..2713
                     /gene="trmFO"
                     /locus_tag="VNY06_00560"
     CDS             1310..2713
                     /gene="trmFO"
                     /locus_tag="VNY06_00560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019401898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate--tRNA-(uracil(54)-
                     C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO"
                     /protein_id="HWX13366.1"
                     /translation="MSVRHHFAPIHVIGGGLAGSEAAWQIARAGVSVVLHEMRPKRQT
                     AAHQTASLAELVCSNSFRADDPQTSAIGILHREMRQLDSLIMLAADAHKLPAGGALAV
                     DRNAFSARITQAISSHPLITVAREEIDNLPPEEWDNVIVATGPLTSPSFSSAISKLTG
                     ERDLAFFDAIAPVVYRASLDMEKAWFQSRYDKAGPAGTGADYINCPLTQAQYYALVDA
                     LIAADRAETHAVAEESTPYFNGCLPIEIMAERGRETLRHGPMKPFGLTNPHPCAQKPY
                     AVVQPRQDNALGTLYNMVGFQTKLKHLAQVETFRTIPGLENASFARLGGIHRNTFLNA
                     PKLLDRGLCLKSDPRLRFAGQITGCEGYVESAAIGLSAGRMATAERKGEIFATLPATT
                     AVGALINHITGGHIEIIDAGPPSFQPMNINFGLFPPLAAGDANSEKSLGRKAALSLRA
                     ARELDAWLGGKQSCAAE"
     gene            complement(2732..3667)
                     /gene="ubiA"
                     /locus_tag="VNY06_00565"
     CDS             complement(2732..3667)
                     /gene="ubiA"
                     /locus_tag="VNY06_00565"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012385219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HWX13367.1"
                     /translation="MAEVLLPPASLPDSLQGQLIFRLAPKAWHPFFQLARLDRPAGWW
                     LLLLPCWWSSALASISQGAPLHVRDLLLFFIGAVVMRGAGSTYNDIVDRDIDAKVVRT
                     QTRPLASRRVSVKSAALFLVAQCLAGLTVLLSFNRFTIFLGFASLGVVGLYPFMKRIS
                     SWPQAVLGAAFAWGALVGWSAAFGSLAAAPILLYAGAVFWTVGYDTIYALQDIEDDVL
                     AGIGSTARAFGRHVRSGVLALYVVATVCIEGALLTVSAGWLAQGGFVLFAGHLAWQVV
                     QIDPGNGALALRLFRSNRDAGLALFAGLVAETWLR"
     gene            complement(3737..4180)
                     /locus_tag="VNY06_00570"
     CDS             complement(3737..4180)
                     /locus_tag="VNY06_00570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012591888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX13368.1"
                     /translation="MMPLKRVGSARKALLAAISGLFFFICGAAYAIDCNQDIGALTKK
                     RQGIIDQLNHLAQGGKKQLDPIASCPKLRALVAAERELVAYLAKNKDWCAVPDEAFQN
                     ISASAGKSSKVANQACTVAAQMKKAQEQQASGALNAQQKLPGGPL"
     gene            4382..5137
                     /locus_tag="VNY06_00575"
     CDS             4382..5137
                     /locus_tag="VNY06_00575"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020176776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HWX13369.1"
                     /translation="MPRYDFAAQRLFVTAPLAPEAKIDLDQTQANYVRNVLRLTEGAK
                     ILVFNGQDGEWETSVVAANRKRVTLAVHKMTRPQQPPSSLQYLFAPLKHARQDYMIEK
                     AVEMGAGLLRPVVTCRTQTTRINSERMRFHAIEAAKQCGILSIPDIAPPMRLDAVLGK
                     WDRDCLLIFCDEDAPVENPYEILRSCRSPGLSRKLAVLIGPEGGFDPSERESLLSLPW
                     IVRLPLGPRILRADTAAVAALALLQATLGDWQG"
     gene            5219..5563
                     /locus_tag="VNY06_00580"
     CDS             5219..5563
                     /locus_tag="VNY06_00580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX13370.1"
                     /translation="MNFRKILVSIGCFAAAIGIGSGKPARAGDFQSDNDPVSSPVEPC
                     AVRSARDGQFGGCERIGGHVRIDMVPRIQDTSGFDGPKASPVAVRMDDGTGPRGHLHL
                     PSGLDELDPIRR"
     gene            complement(5591..5944)
                     /locus_tag="VNY06_00585"
     CDS             complement(5591..5944)
                     /locus_tag="VNY06_00585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX13371.1"
                     /translation="MGSARNRGGHACCPTGKYFFAPLQKDMHPPEAPVAANGKGFFVA
                     WTCSNGDDCGGNSIMVYFDSVAGVAQVCLRSEGSDGKVQDLWLANGEARPLAINGCGV
                     GERDPFASPKRFGGK"
     gene            complement(6128..7510)
                     /locus_tag="VNY06_00590"
     CDS             complement(6128..7510)
                     /locus_tag="VNY06_00590"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012590848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HWX13372.1"
                     /translation="MARDVTDSTPISSRSELVAWIEAGAKPPEEFRIGTEHEKIPFYR
                     RGNLPVPYEGLPQLGRGGIRALLEGMQEKLGWAPVTDRAQIIGLYDARSGAGISLEPG
                     GQFELSGGPVETIHETDAELEAHFSAIKTVAALLGIDFLSLGESPKWALNEIPLMPKQ
                     RYAIMARYMPKVGWRGLEMMFRTCTVQANFDYSGEADMVKKLRVSLALQPVATALFAN
                     SPLSEGKLNGFLSDRSEIWRHTDPNRTGMLPFVFEPGMSFERYVDYALSVPMYFVKRG
                     DIYHDVAGANFDDLLQGRLTQLPGERATLADWANHLSTIFPEVRLKRYLEIRGADSGP
                     RPFLTALPALFAGLLYEQNALNAAWDLVKVWSEVDREQLRTAVPRLGLDAEIGGRRLR
                     KVAAEVLCIARAGLARRNRPGPRGQDESFYLAPLDAVLARGSEAERLIRMFETQWTGS
                     VDGAFEECVY"
     gene            complement(7643..>8083)
                     /locus_tag="VNY06_00595"
     CDS             complement(7643..>8083)
                     /locus_tag="VNY06_00595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX13373.1"
                     /translation="GFGGILGQLSHAASQGSLGPVLGQILGGGPAPASPPQPAQVPGP
                     LAGVGGLGGILGAILASLKGQAGASRNAGPATSGASPEQTPSFDAATIQAGLEALKKI
                     LQPGTPPALHSQPETPTAPAPRTEGQQTDISAELDRIVGRNTGL"
BASE COUNT         1769 a         2429 c         2233 g         1652 t
ORIGIN      
        1 atcgtgtgtt tttccggatc cttaacgaaa cgcccaagcg tccaaggaag gacgcggtat
       61 cggaaacaac acggaaacgt ctggaagcat taaatcaata cgacatcgag ttgtatgaat
      121 gggtgaagga acgtttcgcc aagcaaattg agcctctaga gcctgacttt tcgcgggaag
      181 ttcgtcggtt cgagatgcta aaccgctccg tccgtcggat tcgccagctc accccccgac
      241 caattcacag gacgatgaag cagctgctgt tgtcaccgca gcgcttatcg caatggtttt
      301 gcgcacatgc tctatttgcg atatgcgaca cagctttttt ccgattagat tattcgactg
      361 tgctggtagc tagcttctaa gccagcgacg ggccgaggct ctacataccc gatgagctta
      421 cgaccggatt gcatgtccac gacgtcgcca aacttttaga cgtgctgcac gagctcgccg
      481 accagggcaa aaacagccac acggggcgtt acttgcgcga attcctgtaa aggcggcctt
      541 tggccaagga caagcaggcg gcagagtagc tttgcttggc cagtttaaag tcacgcatag
      601 cgaggatctc cgcacaggaa acgctttttt gccaggacgg aggacaagcc cggcgcaaga
      661 tcaagccgct actcctctta caacacgcca gtttgtggtt aggtgcagta aaaaatcaga
      721 aacactgcac aattcgtccc tcggaccttc ccaaaggacg gggcgaggtc tggcagatgc
      781 aacatggcag gagttttgct aggtgagtga cagggcaagc gcaggctgca cgtgttttgg
      841 cctcaaacct tgttgacggt caaggtttgg gagttgaagc actggtactg gcaaagcttt
      901 gttacagcta ccgtataaaa actaagtctc ggtcataaat taagctcaga tcgcaattaa
      961 aggctcagtc ccttggttta acacggtaac ggtcaaaaat cggttgtgcc ggcggggctc
     1021 tggtggctct aacgcccttc gccagccaga agcgcccgct gctcctcccc ggcgggcgct
     1081 ttctttttgg cgtgccggct cgcattcttg aagctttggc gcagccaaag agttttgagc
     1141 caaacgcggc ctctggtttt gcgcctggac acccgacaaa tcgaagaatt ggagcaagag
     1201 cacgatttaa gaaaagtcgt cttgctcgaa aagcggaact tctccatttg gggacaagcg
     1261 cagccacgaa gggtttgcgc tgatccggct ttgcgctaaa tctcagccaa tgtctgtgcg
     1321 ccaccacttt gctcccattc acgtgattgg cggcggcctc gctggcagcg aggccgcgtg
     1381 gcagattgcg cgggctggcg tttccgtcgt cctgcacgag atgcggccga aacgccagac
     1441 agcggcgcac caaaccgcga gcctcgccga gctcgtctgt tccaattcct ttcgcgccga
     1501 tgatccgcag acgagtgcga ttggcatttt gcatcgcgaa atgcggcagc tcgactcgct
     1561 catcatgctt gccgccgatg cccataagct gcccgctggc ggggcgctcg cggtggatcg
     1621 caacgcgttc tcggcccgca ttacgcaggc gatctcctct cacccgctta tcactgttgc
     1681 gcgcgaggaa atcgacaact tgccgccgga agagtgggat aatgtcattg tcgcaacagg
     1741 gcctttgact tcaccctcct tttcttcggc gattagcaag ttgaccggag agcgggatct
     1801 cgcatttttc gatgccattg cgcctgtcgt atatcgggct tcgctcgaca tggaaaaggc
     1861 gtggtttcag tcgcgctatg acaaagcagg gccagctggg accggggccg attatatcaa
     1921 ttgcccgcta acccaggccc agtattatgc cttagtggac gctctcatcg cagccgacag
     1981 ggccgagacc catgcggttg cggaggaaag cacgccctat ttcaacgggt gcctgccaat
     2041 cgaaatcatg gcggagcgtg gccgcgagac tcttcgacac ggcccaatga aaccatttgg
     2101 tctcaccaat ccgcatcctt gcgcgcaaaa accttatgcg gtcgtgcagc ctcgtcagga
     2161 caatgcgcta ggcaccctct acaatatggt tggtttccaa accaagttga aacatcttgc
     2221 gcaggttgaa actttccgca ccattcctgg cctagagaac gccagtttcg ctcgtctcgg
     2281 aggaatccac cgcaatactt ttctgaacgc acccaaattg ctcgatcgcg gcctgtgtct
     2341 aaaatccgat ccaaggttgc gcttcgcagg ccaaatcacg ggctgcgagg gttatgtcga
     2401 aagtgccgca ataggactct ccgctggacg catggcgacg gcagagcgta aaggcgaaat
     2461 tttcgcgacg ctccctgcga cgactgccgt tggggcgctc atcaaccata ttaccggcgg
     2521 acatattgag attatcgacg ccggaccgcc ttcgtttcag ccgatgaaca ttaattttgg
     2581 gttgttcccg cctttggccg cgggtgatgc gaattcggaa aagtcgctag gccggaaggc
     2641 ggcattaagc ttgcgggccg cacgcgaatt ggacgcatgg cttggtggca agcaaagttg
     2701 tgcggctgag taaccgccgc agccgatcac ttcagcgcaa ccaggtctcg gccaccaaac
     2761 cggcaaacaa tgcaaggccc gcatcccggt tcgaccggaa caagcgcaac gcgagggcac
     2821 cattgccggg atcaatctgc acgacctgcc aggcgagatg tccggcaaaa agcacaaaac
     2881 ctccttgcgc cagccaaccg gcactgacgg tcagcaatgc cccttcgatg caaacggtgg
     2941 cgaccacgta gagcgctaaa acccccgaac ggacatggcg gccaaatgca cgcgcggtcg
     3001 aaccaatgcc cgcaaggaca tcgtcttcga tgtcctgcag cgcatagatc gtgtcatagc
     3061 caacggtcca gaaaaccgcg ccggcataga gcaaaattgg cgctgccgcg agcgagccga
     3121 aggccgccga ccacccgacc aaagcgcccc atgcgaaggc cgcgcccaga accgcttgcg
     3181 gccaggagct gatccgcttc atgaatggat agaggccgac gacgccaagc gaggcaaatc
     3241 ccaaaaagat cgtaaacctg ttaaaactta acagcacggt aagcccggca agacactgcg
     3301 ccaccaggaa caacgcagcg ctttttacgc tgacgcgcct tgaagcgagt gggcgcgttt
     3361 gcgtgcggac taccttcgca tcgatgtcac gatcaacgat atcattatag gtcgacccgg
     3421 cgccccgcat gacgacggct ccaatgaaga acaaaagtaa atcacggaca tggagcggcg
     3481 ccccttgcga tatgcttgcg agtgcgctcg accaccagca cggcagcaat agaagccacc
     3541 aaccggccgg acggtcgagg cgcgcgagct gaaagaatgg atgccaggct ttaggcgcca
     3601 gccggaagat gagttggcct tggagcgaat caggcaaaga ggctggcggc agcaaaactt
     3661 ccgccacagc ggtcttgaca tcgcccgcta ctggctcgtc acgcacgcct ttgacttccg
     3721 gctccatcgc gcggcgtcac agcggcccac caggaagctt ttgctgtgcg ttgagggcgc
     3781 ccgacgcctg ctgctcttgc gctttcttca tctgtgccgc gaccgtgcag gcctgatttg
     3841 cgactttgct ggatttgccc gcgcttgccg aaatattttg gaaggcttca tccggaactg
     3901 cgcaccagtc cttgttcttg gcgagatagg ctacgagttc acgctcagcg gcgacaagag
     3961 cccgcagctt tggacaggaa gcgatgggat cgagctgctt tttgccaccc tgggcaaggt
     4021 gatttagttg atcgatgata ccctgcctct tcttagtcag ggcaccaata tcttggttgc
     4081 aatcgatcgc ataagccgcg ccgcagataa agaagaaaag cccgctgatg gcggcaagca
     4141 gcgctttacg cgcggagcca acacgcttaa gtggcatcat gatcctagcc tttcagtgtc
     4201 atgccgatgt gctgtgaaga ctttctatta aaccagccaa attccggagc cttgcattgc
     4261 ctttgcgaac cagacgccaa atcacagctt gcaagtttct gcaagcacgg ctgagacgcg
     4321 gccgcgcaca aaccggaccc tcgcgccgcg ctgtggccga taaaacctag cgggcgcccg
     4381 catgccccgc tacgactttg cagcgcagag gctctttgtc acagccccac tcgcgccgga
     4441 agcgaagatc gatcttgatc agacacaggc aaattatgtc cgcaatgtgt tgcgcctaac
     4501 ggaaggtgcg aaaatccttg ttttcaatgg ccaggatggc gaatgggaaa ccagcgttgt
     4561 cgccgccaat cgtaaacgcg taacgctcgc tgttcacaaa atgacccggc cgcagcagcc
     4621 gccatcctct ctccaatatc tttttgcgcc gctcaaacat gcgcgccagg attatatgat
     4681 tgaaaaagcc gtggaaatgg gggctgggct tctgcgcccg gtcgtcacgt gccggaccca
     4741 aacgacgcgc atcaatagcg aacgtatgcg cttccatgcg atcgaagccg ccaagcaatg
     4801 cggtattctg tccattccgg atatcgcgcc acccatgcgg ctcgatgccg ttctcggcaa
     4861 atgggatagg gattgcctcc ttattttttg cgatgaagat gcgccggtcg aaaaccctta
     4921 cgagatattg cgttcctgcc gcagcccagg attatcgcgc aagctcgccg ttctcatcgg
     4981 cccggaaggg gggttcgacc cgagcgagcg cgagtcgttg cttagtttgc cttggatcgt
     5041 ccgcctgccg cttggcccgc gtattttgcg cgccgacacc gcagcagtcg cggctcttgc
     5101 cttgctccaa gctaccctcg gcgattggca aggataaatc ttaacacatc attatatcgc
     5161 ctcattgtag cgtcaccaaa ttcgggccca gcccgtcaac gaatcgttta ggaatcaaat
     5221 gaatttccgc aaaatcctcg tttctattgg ctgttttgcc gcagccattg gaattggttc
     5281 cggcaaacca gcacgggccg gtgatttcca atcggataat gacccggttt cgagtccggt
     5341 tgagccttgc gccgtccgga gcgcgcgcga cggtcaattt ggaggctgtg aacggatcgg
     5401 cggtcatgtg cgcatcgata tggtcccccg tatccaggac acttctggat ttgacgggcc
     5461 aaaggcatcg cccgtcgctg tccgaatgga cgatggaacg gggccgcgcg gtcatcttca
     5521 tttgccgagc ggcctagatg agcttgatcc tatccgccgc tgatattagc tccgcggttg
     5581 gcgcctcccc tcacttcccg ccaaacctct taggggacgc aaaaggatct ctttcgccaa
     5641 caccgcagcc gttgatggca agcgggcgcg cctcgccatt tgcaagccat aggtcttgca
     5701 cttttccgtc agagccttcg gagcgcaagc agacctgcgc gacgccggcc accgagtcaa
     5761 aatagaccat tatactattg ccgccgcaat cgtccccatt gctacaggtc cacgcgacga
     5821 agaagccctt gccattggct gccaccggcg cttccggagg gtgcatgtct ttttgcagcg
     5881 gcgcaaaaaa atatttgccc gtggggcagc acgcatgacc tcctcgattc cgggctgatc
     5941 ccaaagcggc tttccgtcga cgatttcatc ggatgggtat tggccgaccc attgcccgac
     6001 gaccgtcgag agatcggccg ccatcacagc cttgcagcaa agacaggccc aagccgggca
     6061 acaccttcct ggcaaacgac gtcatttgct cctcctgacg ctgccgcttc gagacaaaat
     6121 tagggcctta atagacgcat tcctcaaaag ccccatcaac gcttcctgtc cattgcgtct
     6181 cgaacatcct aatcagccgt tcagcttcgc tcccccgcgc tagaaccgcg tcgagagggg
     6241 caagataaaa ggactcgtcc tgccctcttg ggccaggccg gttgcgcctg gccagccccg
     6301 cacgggcaat gcacaagact tctgcggcga ccttgcgcag ccgccgcccg cctatttccg
     6361 catcaaggcc gagacgcggg accgccgtgc gcagctgctc tcggtctact tcgctccaaa
     6421 ccttcaccag gtcccaggca gcattcagcg cgttctgctc ataaagaagc cccgcgaaaa
     6481 gcgctggaag cgcggtaaga aaagggcgag gccccgaatc ggcgccgcgg atttcgagat
     6541 agcgcttcag ccggacctcc ggaaaaatgg tcgaaaggtg attggcccag tcagccagcg
     6601 tcgcacgctc accgggaagt tgcgtaagcc tgccttgcaa aaggtcatca aaattcgccc
     6661 cggccacatc gtgatagata tcgccgcgct tgacaaaata cataggcacc gacagagcgt
     6721 aatcgacata gcgctcgaac gacattcctg gttcaaaaac aaaaggaagc atgccggtgc
     6781 ggttcggatc cgtgtggcgc caaatctccg atcggtccga aagaaagccg ttaagcttgc
     6841 cctcactgag cggcgaattg gcaaaaaggg ccgttgcaac cggctgcaag gccaacgaca
     6901 cccgaagttt tttaaccatg tcggcttcgc cggaataatc gaaattggcc tgcaccgtgc
     6961 aagtgcgaaa catcatttcg agtccgcgcc atccaacctt tggcatgtat ctcgccatga
     7021 tcgcgtagcg ctgcttgggc atcaacggga tttcgttaag tgcccacttg ggactttcac
     7081 cgagtgacag gaaatctatc ccgagaagcg cggcaacggt cttgatggcc gaaaaatggg
     7141 cctcaagttc cgcgtcggtt tcatgaatcg tctcaacagg cccgccggag agttcgaatt
     7201 ggccgccggg ctcaagcgaa atccccgccc cgctccttgc atcataaagt ccgatgattt
     7261 gcgctctgtc ggtaaccggg gcccagccca atttctcttg catgccctcg aggagagctc
     7321 ttatcccgcc gcgaccaagt tgcggcaggc cttcataagg taccggcaaa ttgccgcgac
     7381 ggtagaacgg gattttctca tgttccgtgc cgatccggaa ctcttccggc ggttttgctc
     7441 cggcctcgat ccacgcaaca agctcgctgc gcgacgaaat gggggttgaa tccgtaacgt
     7501 cgcgcgccat ttcacacttt ctctagcgcc agatcccggg cccaccggct gcgctaccgt
     7561 gccgctaaat aggcggacgc gcgccggagt gcaaacttcg ctggcaatag agtttgccgc
     7621 accgccagag atgggaccgt cgctataggc cagtattcct gccaacgatc cggtcgagtt
     7681 cggcgcttat gtctgtttgc tgcccttccg tccttggagc gggagccgtt ggtgtttccg
     7741 gctgcgaatg taaagccggc ggtgttccgg gctgcagtat ctttttcagc gcctcaagac
     7801 ccgcctggat ggtcgcggca tcgaaactgg gcgtctgctc cggagaggca ccggaagtgg
     7861 ctggtcccgc gttccgagaa gcacctgctt gacctttaag acttgccaaa atggcgccta
     7921 agataccgcc aagccctccg actccagcca acggaccagg cacttgggcc ggttgcggcg
     7981 ggctcgctgg cgccggtccg ccccccagaa tttggccgag aacggggcca aggcttcctt
     8041 ggctggcagc gtgcgaaagc tgtcccaaaa ttccgccaaa acc
//
LOCUS       DATMPU010000028         6003 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_1569975,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000028 DATMPU010000000
VERSION     DATMPU010000028.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 6003)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6003)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6003
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1569975"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(568..3819)
                     /locus_tag="VNY06_00600"
     CDS             complement(568..3819)
                     /locus_tag="VNY06_00600"
                     /inference="COORDINATES: protein motif:HMM:NF014442.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HWX13374.1"
                     /translation="MDLFAAAGIDLGDGGRGGRPGRSGVGRTLRVPLLSYKVIAEALK
                     RVNFEPTKEQLEIAQKYAEAARSPRFRANETRIRNRFYDEILGTLLGYRKYDPDQTYT
                     IDFEHSTRGGSADVALGHFSERGATNEVIAPFEMKGPLTVDLDAIPPGGRRSPVQQAW
                     DYAIDTPGARWVLVSNCLEIRLYGFGRGRDAYELFDLTKLDDPGEHEKLWLVLSAVRL
                     LTGMTDTLLRDTDNAYKDITDKLYVEYKTLRDRLIAFLTGAADGPKLPMKGAIEVAQK
                     SLDRILFIAFAERTDLLPMKLLERAANARNEFRPEPYWKNFQALFHEIDVGNEGRGIW
                     AYNGGLFAPDEVADAVVIPDPLATDLGALGQWDYKSDVPVTVLGHIFEQSVTDLEKLR
                     AESRGEEPPKISKRKREGVVYTPDIVTRFLVERTIGLTLSERFSAILTAHGRKLPKNG
                     DPIFGEKEKDAERSFWRDYAGSLRSLRVVDPACGSGAFLVAAFDLFAREYRRVVTHLI
                     ALGESVDFDPFDEILTKNLHGVDLNAESVEITRLALWLKTARNKHRLQNLEATIKVGN
                     SLIDDRTFTDQPFDWRTAFVDVPDGFDIVIGNPPYVRMGLIKSVKPYLEKHYVVADDR
                     TDLYAYFFERGVRLLKSGGRLGYISSSTFFRTGSGENLRKFLGDNVAIEAVIDFGDLQ
                     LFEGVTTYPAIITLRKGGTGTQGALSFLKVREELPKDLDAEFTAKATTMPRARLGSGS
                     WQFEGDALARLRDKIIKGKKTLGEVYGAPLYGIKTGLNNAFIIDQETYDRLVKRDKNS
                     AKLLKPFLRGENIKRWRVEPEGLFLINTPKDKVDIDGYPAIRDWLLPFKPELEKRATK
                     QEWWELQQAQLAYQPRFSTPKIIYGHFCQSRNFAFDDEGYFSNDKSYFIPNSSFDLLA
                     LLNSRVAWSFIASISPAVRGGFHEMRVQYLEKVPIPKIATRERNSLTRLGEGNTTAAQ
                     RRLEIQSKIRHRILTDFATPERQKFSRKLEEWWTLDFGGFRAEVKRVFHTEIPVKERG
                     EWEAYLASQAAEVRKLDAAIDKAEREIDAIVYRLFDLTPDEIALLEASIARQY"
     gene            4009..4224
                     /locus_tag="VNY06_00605"
     CDS             4009..4224
                     /locus_tag="VNY06_00605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013503821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1902 domain-containing protein"
                     /protein_id="HWX13375.1"
                     /translation="MSKPVIFTISTAWDDEASVWSGHCDEIPAAADAPTLDELLAKIS
                     AMALDLLPDNHPDIDPAPLSFSNFRFV"
     gene            4228..4512
                     /locus_tag="VNY06_00610"
     CDS             4228..4512
                     /locus_tag="VNY06_00610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX13376.1"
                     /translation="MASARQGDLFEHDGPDPSEDDFETPVYHADPEKVREELHRILAE
                     ARAAKAMPWDAKRAALYRTIFPQMTGCLPEDEGAQLRFEFETELARLEAA"
     gene            complement(4776..5177)
                     /locus_tag="VNY06_00615"
     CDS             complement(4776..5177)
                     /locus_tag="VNY06_00615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015316286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HWX13377.1"
                     /translation="MIFLDTSAVVAMLAREADGPSLADKLEGADERITAGHVILESAM
                     RLSTLFHISPSAADTRVTALLRESSVDVVPITEAIAHGAVTAFERYGKGSKHRASLNF
                     GDCLSYACAKAHGAKLLFKGEDFVHTDVARA"
     gene            complement(5174..5407)
                     /locus_tag="VNY06_00620"
     CDS             complement(5174..5407)
                     /locus_tag="VNY06_00620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015316285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapB family
                     antitoxin"
                     /protein_id="HWX13378.1"
                     /translation="MALHIRDNRAGRLAKQLAARKGITMTRAVVEALEGALAREERPL
                     RERIADIANELDRLGNRGRAVGKREIDALWGNK"
BASE COUNT         1222 a         1728 c         1758 g         1295 t
ORIGIN      
        1 tcgcgcttaa gcgaagcacc tcgcgcgccg gtcataatgc cggcggggtc gatgcccgga
       61 ccgcccggga gcggcagtga cgagccgccg cccgcaggaa ccgcacccgc cccgtcagtc
      121 ggcgtcaccg gttgacgtcc cttacgcgag cgagattgcg ccggttctca tgaaaagcgg
      181 atgggtgtca atatgaacgt gacggcgcat tccgggccgt tatgggcgtg cgaaaattgc
      241 gtattcatgc gggttttcgc aagcgcgcga atgcgcggcc ggcgcagtga tgctgagcca
      301 cagcgtgggc cggcggtcaa atcgcgcagt caggtgcatc gggcgaaatg cgaaaagtcg
      361 ttgaatggca gggacttctg ctcggcttgc gaatctttct tttgagtgcg gggcggaaac
      421 ggctcaacag atcgtgcggg ttcgactcgg cgccgagcaa gcgtgcgtgc tgctcgaccg
      481 caatgatggg caacatgcgg tcataggttg ctccgacaag cagacatagc ccgcattgag
      541 cgaggggcaa tgcgggacca tgggcgttca atattgcctg gcgatcgagg cttcgagcag
      601 cgcgatctcg tcgggtgtca agtcaaagag gcgatagacg atggcatcga tctcgcgctc
      661 ggccttatcg attgcggcat cgagcttgcg cacttcggct gcttgactgg caagataggc
      721 ttcccattcg ccgcgttcct tgacagggat ttctgtgtga aaaacgcgct tcacctcggc
      781 gcgaaagcct ccgaaatcga gcgtccacca ttcctccagc ttgcgcgaaa atttctgtcg
      841 ctcgggagtg gcaaagtcgg tgagaatgcg atgtctgatt tttgattgga tttcaaggcg
      901 ccgctgcgcg gcggtcgtat ttccctcgcc aagccttgtc agactattcc gctcacgagt
      961 cgcgatctta ggaatgggga ccttctccaa gtattggacc cgcatttcat gaaagccgcc
     1021 acgcaccgca ggtgatatgc ttgcaataaa ggaccaagct acccgtgagt taagtagggc
     1081 taggagatcg aacgaggaat ttggaatgaa gtacgactta tcattggaga agtacccctc
     1141 atcatcaaat gcaaaatttc tgctttggca aaaatgccca taaatgattt taggcgtgga
     1201 aaatctcggc tgatacgcca attgggcctg ctgcaattcc caccattcct gcttggttgc
     1261 gcgcttctcc agctccggtt tgaacggcaa tagccagtcg cggatcgctg gatagccgtc
     1321 aatatccacc ttgtccttcg gtgtgttgat gagaaaaagg ccctccggct cgacgcgcca
     1381 gcgcttgata ttctcgccgc gcagaaatgg cttcagcaat tttgctgaat ttttgtcccg
     1441 tttgacaagc cgatcatagg tctcttggtc gataataaaa gcgttgttga gtccggtctt
     1501 gataccgtag agcggtgcgc cgtagacctc gcccagcgtc ttcttgccct tgatgatctt
     1561 gtcgcgcagc cgggcgagag cgtccccctc gaattgccag gagcctgacc cgagccgtgc
     1621 acgcggcatt gtcgtagcct tcgcggtaaa ttcggcgtct aggtccttgg gcagttcctc
     1681 acggacttta agaaacgaaa gagcgccttg ggtgcccgtt ccgccttttc gcagggtgat
     1741 gatggcagga taggtagtca cgccctcgaa caattgtaga tcgccgaaat cgattaccgc
     1801 ctcaattgcg acattgtcgc caagaaactt gcgcaaattc tctcccgagc cggtgcggaa
     1861 gaatgtagat gaggaaatgt aacccagccg accgcctgat tttaggagtc tcacgccgcg
     1921 ctcaaaaaaa taggcataga gatcggtgcg gtcgtccgcc acgacgtagt gcttttccag
     1981 atacggtttg actgacttaa ttagtcccat gcggacatag ggcggattac cgatgacgat
     2041 atcgaagccg tcgggcacgt caacaaaagc cgtccgccaa tcgaagggct gatcggtgaa
     2101 agtgcgatcg tcgatcaggc tgttgcccac cttaattgtc gcttcgagat tttgcaggcg
     2161 gtgtttattg cgcgctgttt tgagccacag cgccagccgt gtgatttcga cagattcggc
     2221 attaagatcg accccatgca aattcttggt gagaatctcg tcgaatggat cgaagtcaac
     2281 ggactcgccg agcgcgatca aatgggtgac gacccggcga tactcccgcg cgaacaggtc
     2341 gaaggccgcc acgaggaatg cgccggagcc gcatgcaggg tcaacaaccc gtaggctacg
     2401 caacgaaccg gcgtagtcgc gccaaaatga gcgctcggcg tctttctcct tttccccgaa
     2461 aatcgggtcg ccgttcttcg gcaatttcct gccatgcgcc gtcaaaatcg cgctgaaacg
     2521 ctcagagagg gtcaatccga tagtccgttc gacgagaaag cgcgtcacaa tatcgggcgt
     2581 ataaacaact ccttcgcgct tacgcttcga gattttcggc ggctcttcgc cgcggctttc
     2641 cgcccttagc ttttcgagat cggtgaccga ttgctcaaag atatggccga gcaccgtaac
     2701 cggaacgtcg cttttgtagt cccactgccc aagagctccg agatcggtgg cgagcggatc
     2761 tggtattacc acggcgtcgg cgacctcatc gggcgcaaac aatccaccat tgtaagccca
     2821 gatgccgcga ccttcattgc cgacatcgat ttcgtgaaag agcgcttgaa aattcttcca
     2881 atacggctcg ggtctgaact cattgcgagc gttcgccgcg cgttccagaa gcttcatggg
     2941 caagagatca gtgcgctcgg cgaaagcgat aaacaaaatt cgatcgaggg acttctgagc
     3001 aacctcgata gcccccttca taggcaactt tgggccatcc gccgcgcccg taagaaacgc
     3061 aatcagtcgg tcacgcaacg tcttatattc gacatacaac ttgtcagtga tatctttgta
     3121 ggcgttgtcg gtgtcgcgca gtaacgtgtc ggtcataccg gtaagaagac ggacggcgga
     3181 caaaactagc caaagcttct cgtgctcccc gggatcgtcg agtttggtga gatcgaaaag
     3241 ctcgtaggcg tcgcgaccac ggccaaatcc atacagcctg atctcaagac aattcgacac
     3301 caagacccac cgggcacccg gtgtatcaat ggcataatcc catgcctgct gaactgggct
     3361 gcgtcgacca cccggaggaa ttgcgtccag atcgacggtg agtgggccct tcatttcgaa
     3421 tggtgcgata acctcattcg tcgctccgcg ctcggagaaa tggccgagcg ctacatcggc
     3481 cgaaccgccg cgcgtcgaat gctcgaaatc gatggtataa gtctggtccg gatcgtattt
     3541 gcgatagccg agcagcgttc cgagaatttc gtcgtagaag cgatttcgga tacgcgtctc
     3601 gttggctcga aagcggggac tgcgcgcagc ttccgcatat ttttgggcga tctccaattg
     3661 ctctttagtc ggctcgaaat tgacgcgctt tagtgcctct gcaattactt tgtaactgag
     3721 caaaggcacg cgtagcgtgc ggccgacgcc cgaacggccc ggtcggccgc cccgtccacc
     3781 gtcgccgaga tcgatccctg ccgccgcgaa aaggtccatg tgaattgtgc aatctattgg
     3841 ggcaagcatc cgaggtcgag atgcgaagtt gggaaactaa caggttcccg caatccgaat
     3901 cccggcttca tacctatcaa cctcggcctc ccccacatag gcaggatatg ataaaatagc
     3961 gcttggttgt ggtgccttcg aggtagcact gcggccaatg gagcgaatat gtcgaaaccg
     4021 gtgatcttca cgatttccac cgcatgggac gacgaggcct cggtctggag cgggcattgc
     4081 gatgagattc cggccgcggc tgacgcaccg acgctcgacg aactgctggc gaagatttcc
     4141 gcgatggcgc tcgatctttt gccggataat catccggaca tcgatccggc tccgctgtcc
     4201 ttttcgaatt ttcgatttgt gtgaaacatg gcatctgccc gccaaggcga tctgttcgaa
     4261 cacgacgggc cagacccgtc cgaggacgat ttcgagactc ctgtctacca cgccgatccc
     4321 gagaaggttc gcgaggagtt gcacaggatt ctcgccgagg cgcgggcggc aaaggcgatg
     4381 ccttgggacg ccaaacgcgc cgcgctctat cgtaccatct tcccgcagat gaccggctgc
     4441 ttgccggagg atgagggcgc gcaattgcgt ttcgaattcg agaccgaact ggcgcggctt
     4501 gaggctgcct aaattttttc tttccggatt tcgctaccgc tcaatccaag ctacgcttag
     4561 cgatccgcgt gaccatggga aaacgcgcga ccaccgggag ggtcccacat gccatccacc
     4621 atcaacctct atcagatcgg cgtctggttc tgcgtcggct tcttcaccgg cgccggttgg
     4681 gcgattgcgg cggtgctggt cgggcgcatc ttgtccgcgg tctgaccgca agcgccggcc
     4741 agtagtccgc attgggccgt cgtcgctggc gcgcctcacg cccgcgccac atccgtgtgc
     4801 acaaaatcct cgcccttgaa caaaagcttc gccccgtgcg ctttggcgca ggcgtaggac
     4861 aggcaatcgc cgaaattcag gctggcgcgg tgtttgctgc ctttgccgta gcgctcgaac
     4921 gcggtgacgg cgccgtgggc gatggcctcg gtgatcggca ccacgtccac cgagctttct
     4981 cgcagcagcg cggtcacgcg ggtatcggcg gcgctcggag atatatggaa caaggtcgaa
     5041 agccgcatcg cagattcgag aatgacgtgg cctgcggtaa tgcgctcgtc cgccccctcc
     5101 aatttatccg ccagtgacgg gccgtccgcc tctcgcgcca gcatggcgac gaccgctgac
     5161 gtgtcgagaa agatcacttg ttgccccaga gcgcgtcgat ctcccgcttg ccgacggcac
     5221 ggccgcggtt gccgaggcga tcgagctcgt tggcgatatc ggcgatgcgc tcgcgcaacg
     5281 gtcgctcctc gcgggcgaga gcgccttcca gcgcttcgac cacggcgcgc gtcatggtga
     5341 tgcctttgcg ggcggcgagt tgcttggcga gacggccggc gcggttgtcg cggatatgca
     5401 gggccatgga gctgctcgtg tgtagacata tctgtgtaga tatctagaca gtgatatata
     5461 cagcagtttt ggcgattact caactggagt ggggcctttc ggctgctttt cgcattcctt
     5521 gagtcagcgg acccccacct agcctccccc tttcaggggg aggaagtgcg agcggcttgc
     5581 gcggaattat cgtccaggtg ccagcgctag gcttgctgct ttctccccct gaaaggggga
     5641 cgattgaggt gggggtcgcg ggccgcttgc gtggcgcgat tggcagcaac atcaatcctg
     5701 ccgccccttt tcccaaccgg aacccgcccc catataggtc attgtcaggg gatggttgag
     5761 gcttgggtcg gtttcttcgt cggttctcgg ccccggcatt tgccaaattc ctgacgccaa
     5821 aactcaccca gtgacgatgt tccggtggtg ccttcggggc ttccgggata ggccgaggga
     5881 ccgaaaaaca tgaatttcga aaagtatacc gaccgcagcc gcggtttcgt gcagtcggcc
     5941 cagtcgctcg cgcagcgcga gggccaccag caatttgcgc ccgaacacct gctcaaggtt
     6001 ttg
//
LOCUS       DATMPU010000029         6356 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_639409,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000029 DATMPU010000000
VERSION     DATMPU010000029.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 6356)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6356)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6356
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_639409"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..190
                     /locus_tag="VNY06_00625"
     CDS             <1..190
                     /locus_tag="VNY06_00625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018406591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="iron permease"
                     /protein_id="HWX13379.1"
                     /translation="WNTSWILSDNGITGRVLHTLIGYTGKPTQMQLLVYLATLGTIFG
                     LKKLLSPPAGQKPKLATN"
     gene            567..2075
                     /locus_tag="VNY06_00630"
     CDS             567..2075
                     /locus_tag="VNY06_00630"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020175637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HWX13380.1"
                     /translation="MSFAKLGLSEKVLQAVAASGYLEPTPIQAEAIPHVLAGRDVLGI
                     AQTGTGKTAAFTLPMLSRLERGRARARMPRTLILEPTRELAAQVEESFVRYGINHKLN
                     VALLIGGVSFGDQDSKITRGADVLIATPGRLLDFSDRGKLLLTAIEILVIDEADRMLD
                     MGFIPDIERICKLVPFTRQTLFFCATMPPEITRLTKTFLHNPARIEVARAATTAATIS
                     QGLVASASGPAKRETLRRLIRGTSDFKNAIIFCNRKRDVAILHKSLQKHGFNAGVLHG
                     DMDQRARTASLEAFKEGSVELIVCSDVAARGLDIPDVSHVYNFDVPTHAEDYVHRIGR
                     TGRAGKTGVAVSIVTPADKKYIIEIERLIARKIDWLPGESLASFPEAAETGQRPLSGA
                     RAHRSRRTFPPGGDLTRTRGSVSPSFQMGEPSDPKPNHTKRSNRGHSSTEAETKPPVA
                     PKARRRRDYDDDDAPVVGMGDHVPMFLQRPAKSKVEKTPVDKGDPGTKTQAG"
     gene            complement(2088..2465)
                     /locus_tag="VNY06_00635"
     CDS             complement(2088..2465)
                     /locus_tag="VNY06_00635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012591212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly accessory protein"
                     /protein_id="HWX13381.1"
                     /translation="MADPQFAVLSLTEAAAARARTLIESAGRPVAGLRVGIKKGGCAG
                     MSYTMDLAPDIEPGDEVIEDQGVKILISPKDLMFLLGTKLDFAVNKLEASFTFQNPNQ
                     TGACGCGESVSITPSADQRSEAG"
     gene            complement(2504..2650)
                     /locus_tag="VNY06_00640"
     CDS             complement(2504..2650)
                     /locus_tag="VNY06_00640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX13382.1"
                     /translation="MNKTAREDGAIAQGEGRKRVPLFEPARRMSSGSLAEAFSRRLDA
                     RDAI"
     gene            complement(2748..2972)
                     /locus_tag="VNY06_00645"
     CDS             complement(2748..2972)
                     /locus_tag="VNY06_00645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX13383.1"
                     /translation="MNVREGLLALVPKVRRTAHLRDGVWRDRLSSGAAAHERRSLLAG
                     HGARAEDQAIGSDFQARMEAPQANPRLPFS"
     gene            3012..3305
                     /locus_tag="VNY06_00650"
     CDS             3012..3305
                     /locus_tag="VNY06_00650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX13384.1"
                     /translation="MLRLFLAEECFSAGITPAELMNAQGRARRERTRKWPLERAAHLV
                     TAGISWAQIRNLIQVLVQAWRRFHLEGKAPGKTTAKPESEPIPSLDSRITRYG"
     gene            complement(3411..>6356)
                     /gene="mfd"
                     /locus_tag="VNY06_00655"
     CDS             complement(3411..>6356)
                     /gene="mfd"
                     /locus_tag="VNY06_00655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003614373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HWX13385.1"
                     /translation="PIRLDFFGDALESIRAFDPETQRATGQLRALDLVPMSEVQLTSE
                     TIRRFRLGYVAEFGAQTRGDALYEAVSEGRRHQGLEHWLPLFYEKLDSLFDYCEGVPL
                     IFDHLTESAANERFAQIEDYYGARKSALDADPGHCPLKPLPPGSLYLTRSEWSSRIGA
                     ASLVSLAPFAQTETGKGRLADCGSRPGRVFAAERADPNANVFSAAISHVRALQTSGKR
                     VMLAAWSDGSRERLAHVLADHGFTATAPVSSLAQALALPPATLALAAVSIEQGFEAPA
                     LAVISEQDILGDRLLGGRRRPRRAEHFLTEAGALTAGDLVVHIDHGVGRFAGLQTIEA
                     AGAPHDCLELHYAEGAKLFLPVENLELLSRYGSEETEIEIDRLGGVAWQRRKARMRKR
                     ILEMAADLIKIAAARQTKAAPKLAPPEGLYAEFCAGFPYEETADQQAAIDAVLDDMAS
                     GRPMDRLVCGDVGFGKTEVALRAAFTAAIGGKQVAVIVPTTLLARQHYANFVARFAHL
                     PVTIAQMSRMVGAAGLRSAKHGAASGEIDIIIGTHAILGKGVSLKDLGLVIVDEEQHF
                     GVKHKERLKELRAEVHVLTLSATPIPRTLQLALTGVRELSLIATPPVDRLAVRTTISP
                     FDDLLVREALLRERYRGGQSFYVCPRIEDLDEAGAFLRTNVPEVKFIVAHGQLPATQL
                     EARMSAFYDGQYNVLLSTAIVESGLDIPAANTLIVHRADMFGLAQLYQLRGRVGRSKI
                     RAYALFTVPPNRALTPQAERRLKVLQSLDTLGAGFELASHDLDIRGAGNLLGDEQSGH
                     IKEVGYELYQEMLREAVEALHAGIEQPSEETWSPSITLGTPVTIPEDYVPDVHLRLSL
                     YRRLAALQSDTEIESFAAEMTDRFGALPAEVEELMQLVAIKVLCRMAHVEKVEAGPKG
                     IMVAFRDNSFANPQGLVRYIAEQGSRAKLRPDMKIVFIRDFEKKATRLEGTSQILRAL
                     AAIAIKKAA"
BASE COUNT         1354 a         1898 c         1850 g         1254 t
ORIGIN      
        1 gtggaacacg tcctggattt tgagcgataa cggtataacc gggcgcgtgc ttcatacgct
       61 tatcggctac accggcaagc cgacccagat gcagcttctg gtttacctcg ctactctcgg
      121 aacgatcttt ggactcaaga agctcttgtc acccccagca ggacaaaaac ccaagctcgc
      181 gacgaattga caacggcaag gaaagcgaag cctcaatatt ggaaagcggc ttcgcggcgt
      241 ggcatttcag cgcatgcagc tttcgttgct ggcccagtcg gtttttcgtg cgaaacgtgg
      301 aagttgattg cgttgcgccg agataagcga tagggagtaa attatcgcct gaatcatcgg
      361 ctgaagcttt cagctgccag tacttcagaa ccgcttgagc ggcatgtcca aggtacgtgg
      421 cgaaatggtc gaagcgcgga atgtttcgcg aacttggacg gttgttctaa cttcaacgtg
      481 aaaggtcggc cgccagaagc gcggcggtcc tgacatggcc ggtaaggacg gtactggttc
      541 catcacagcc cgaatctggg gaaagaatgt ctttcgccaa acttggtctt agcgaaaagg
      601 ttcttcaagc cgtcgcggca tctggatacc tcgaaccgac gcctatccag gcggaggcaa
      661 ttccgcatgt ccttgccgga cgagacgtgc tcggcattgc ccagacaggt accggcaaga
      721 ctgccgcgtt cacgcttccg atgctttcac gtcttgaacg cgggagggcg cgggcgcgga
      781 tgccgcgcac actgatcctg gagccaacac gggaactcgc cgcgcaggtc gaagaaagtt
      841 ttgtccgcta cggcattaac cacaagctca atgtcgcgct tttaataggt ggcgtttcct
      901 tcggcgacca agactctaag attacgcgcg gcgccgatgt tctgatagct actcccggcc
      961 gccttttgga tttttctgat cgcggcaagc tgcttctcac ggcgatcgaa attctcgtca
     1021 tcgacgaggc tgaccggatg ctcgatatgg gattcatccc ggacatcgag cgaatctgca
     1081 agctcgtgcc tttcacacgc cagacgctgt tcttctgtgc caccatgccg ccggagatca
     1141 cgagactcac caagactttc cttcacaatc cagcgcgtat cgaggtggcc cgggctgcca
     1201 cgacggccgc cacaatttcg cagggcctcg ttgcttcggc aagtggtccg gccaagcgcg
     1261 aaaccctgcg ccgtttgatc cgtggcactt ccgatttcaa gaacgctatc attttttgca
     1321 atcgtaagcg ggacgttgcg atactgcaca aatcgcttca aaagcatgga ttcaacgctg
     1381 gcgtcttgca tggcgatatg gatcagcgcg cgcgcacggc gtccctcgag gctttcaagg
     1441 aaggcagcgt ggaactcatc gtctgttcgg acgttgcggc gcgcggcctc gatattccag
     1501 acgtgagcca tgtatacaat ttcgatgtgc cgacccatgc cgaggactat gttcatcgaa
     1561 tcggacggac gggacgcgcg ggcaagactg gagttgcagt ttccatcgtc accccagccg
     1621 ataaaaaata tatcatcgaa attgagcggc tgatcgcccg gaaaatcgat tggctgccgg
     1681 gagagagtct cgccagtttt ccagaggcag cggaaactgg acagcgcccg ctcagcggcg
     1741 cgcgcgccca cagatcgcgc cggactttcc cgcccggagg cgacctgacg cggacgcgcg
     1801 gcagtgtgtc tccctctttt caaatgggtg aaccctccga tcctaagcca aaccacacga
     1861 aacggtcaaa taggggtcat tcgagcacag aggccgagac caagccgccc gtggcgccca
     1921 aggcgcgacg gcggcgcgac tatgacgacg acgacgcgcc cgttgtgggg atgggggacc
     1981 acgtccccat gtttcttcaa aggcctgcta agagcaaagt agaaaagaca cctgtcgaca
     2041 aaggcgatcc gggtacaaag acgcaagccg gctagactgc gcgagcctta accggcctcg
     2101 ctccgttgat cagcggaagg cgtaatcgag acagattccc cgcagccgca cgcgcctgtc
     2161 tggttcggat tttggaaagt aaaactcgcc tccagcttgt tgaccgcgaa atcgagcttc
     2221 gtgccgagta gaaacatcag gtccttgggc gagatcaaaa ttttcactcc ttggtcctca
     2281 atgacctcgt cgccgggctc gatgtcaggc gccaaatcca tcgtgtagga cataccggcg
     2341 caaccgccct ttttgatccc gacccgcaac cccgcgaccg gccgtccggc cgattcaatg
     2401 agcgtgcggg cgcgcgcggc ggcagcttcc gtcagcgaca ggactgcgaa ttgcgggtcg
     2461 gccatagaat cctccgggac ggtcaaaaaa tcgtcggcct cccttatata gcgtccctcg
     2521 cgtcgagtcg ccgcgaaaaa gcttctgcga gtgatcccga ggacatacgc cgcgcaggct
     2581 cgaacaacgg cacgcgcttc cgcccctcgc cttgcgcgat cgcaccatct tccctcgcgg
     2641 ttttgttcac tgtttatttt aggtaatttt gccgactgcc ggtatgctcc ggacccacgt
     2701 tcgctgcttg aatcacggag ccttgggtgc aacgagtgcg agagctatta cgaaaatggg
     2761 agacgcggat tggcttgggg tgcctccatc cgcgcctgga aatcggagcc gatggcctgg
     2821 tcctcggcgc gggcaccgtg cccggcaaga agcgagcgac gctcgtgggc ggccgcacct
     2881 gacgatagac gatcgcgcca tacgccgtcg cgaagatgcg ccgtgcgtcg gacctttgga
     2941 acgagggcga gaaggccctc gcgcacattc atctaactta tgctgacctc ccgatctacg
     3001 gcgaagatga gatgcttcgg ctattcctcg ccgaggaatg tttttccgcg ggcatcactc
     3061 ccgccgagct tatgaacgcg caagggcgtg cccgccggga gcggacccga aagtggccgc
     3121 tggagcgggc ggcccatttg gtcacggcgg ggatcagttg ggctcagatc aggaatctca
     3181 ttcaggtgct cgtccaagcg tggcggcgct tccatctgga aggcaaagct ccaggcaaaa
     3241 ccacggccaa accggaaagc gagccgatcc caagtctcga ttccaggatc acccgatacg
     3301 gctgaatccg gcagagctat gggccggggg cgaaaaggtc cttgcgcatt ttcatctcgg
     3361 ccgatctgcc attctaccgg tttccatttt cgccgggtca tgatcagcgt tcacgcggct
     3421 ttcttgatcg caatggcagc gagcgcacgc aaaatttgac tggtgccctc gagccttgtg
     3481 gcctttttct cgaaatcgcg gataaacaca attttcatgt ccgggcgcag cttggctcgc
     3541 gaaccttgtt cggcgatata tcgcaccaag ccctgagggt ttgcgaaaga attgtcgcga
     3601 aacgcaacca taatcccttt tggccctgct tcaacctttt cgacatgcgc catgcggcac
     3661 aagaccttga tcgcaacgag ctgcatcaat tcctcgactt cggcagggag cgccccaaaa
     3721 cgatcggtca tttcggccgc aaaactttct atttcagtat cggactgcag cgccgcgagg
     3781 cggcggtaaa gactaaggcg caaatgcaca tctggcacat agtcttcggg gatggtgact
     3841 ggcgtgccga gcgtgatcga aggcgaccac gtttcttcgc tgggctgctc aatgcccgcg
     3901 tgcaaggctt caactgcttc ccgcagcatt tcctgataga gttcgtaacc aacttccttg
     3961 atgtgaccgg attgttcgtc ccccagcaaa tttccggcac cgcggatatc aagatcgtgg
     4021 ctcgctaatt caaacccggc accaagcgta tcgagcgatt gcaagacctt gagccgccgc
     4081 tcggcttgcg gcgtcaaggc tcggttgggg ggcacagtga aaagcgcata ggcgcgaatc
     4141 ttcgagcgcc cgacgcggcc gcgcagctga taaagctggg cgagaccgaa catatccgcg
     4201 cgatgcacga tcagcgtgtt ggctgcaggg atatcgaggc cggattcgac aatcgcggtt
     4261 gaaagcagca cattgtattg cccgtcgtaa aaggccgaca tcctggcttc gagttgcgtt
     4321 gctgggagct gaccatgcgc gacaatgaac tttacctccg ggacattcgt gcgcaagaac
     4381 gctcccgctt cgtcgagatc ttcgattcgc ggacagacat aaaaactctg accgccccgg
     4441 tagcgctcac gcagcagcgc ttcgcgcacg agcaagtcat caaatggcga aatggttgtg
     4501 cggacagcta gccggtcgac cggaggtgtc gcgatgaggg agagttcgcg cacgccggta
     4561 agggcaagct gaagagtgcg cggaatgggc gtcgccgaaa gagtcaacac atggacttca
     4621 gcgcgcaatt ccttgagccg ctctttgtgc ttgacaccaa aatgctgttc ttcgtcgaca
     4681 ataacgagcc cgagatcctt taagcttacc ccctttccga ggatcgcatg ggtaccaata
     4741 ataatatcga tttctcccga ggccgcgcca tgcttggcag acctgaggcc agcggcacca
     4801 accattcgcg acatttgcgc aatcgtgacc ggcaaatgtg caaagcgagc gacaaaattc
     4861 gcgtaatgct ggcgcgcaag taatgtagtt ggtacgatga cggccacctg cttgcctcct
     4921 atcgccgcag taaaggcggc gcggagggcg acctcagtct tgccgaatcc gacatctccg
     4981 cagacaaggc ggtccattgg ccggcccgac gccatgtcgt ccaggaccgc gtcgattgcg
     5041 gcctgctggt cggccgtctc ttcataagga aagcccgcgc aaaattcggc atagaggccc
     5101 tccggcggag caagtttcgg tgccgccttg gtctgccgcg cggcggcgat cttgatgagg
     5161 tccgcagcca tttcgagaat gcgcttgcgc atccgcgcct tgcgcctttg ccacgcgacc
     5221 ccgccaagcc tatcgatctc aatctccgtc tcctccgatc cgtagcggga caagagttcg
     5281 agattttcaa ccggcagaaa aagcttggcg ccttcagcgt aatgcagttc gaggcaatca
     5341 tgcggcgctc ccgctgcttc aatcgtctga agacccgcga atcgcccgac accatgatcg
     5401 atatggacca caagatcacc agcagtaagc gccccagctt ccgtgagaaa atgctcggcg
     5461 cggcgtggcc tgcggcgccc gccaagcagc cggtcgccaa ggatgtcctg ctcgctaatg
     5521 accgcgagag caggcgcctc aaaaccttgc tcgatgctaa cagcggcgag cgccagcgtg
     5581 gctggcggca aggcaagggc ttgtgcgagc gacgacaccg gtgccgtggc ggtaaagccg
     5641 tggtccgcaa ggacatgggc aagccgctca cgcgagccat ccgaccaggc ggcaagcatt
     5701 acccgtttgc cgcttgtctg caatgcacgc acatggctga tggcagcgct aaacacattg
     5761 gcatttgggt ctgcgcgctc ggcggcgaag acgcgccctg gccttgaccc gcagtcggcc
     5821 aaacgccctt taccggtctc ggtctgcgcg aacggcgcca aagaaacaag ggacgccgcg
     5881 ccaatgcgcg agctccactc actccgcgtg agataaagag agcccggcgg cagcggtttc
     5941 aacgggcagt gaccggggtc tgcgtccagc gcgctcttgc gggcgccgta ataatcctca
     6001 atctgcgcga aacgctcgtt cgccgccgat tctgtgaggt gatcgaaaat cagagggacg
     6061 ccttcgcaat agtcgaacaa actatcaagc ttttcgtaaa agagcggcaa ccaatgttcg
     6121 agcccttggt gacggcggcc ctcactcacc gcttcgtaaa gggcatcgcc gcgtgtctgg
     6181 gcgccgaact cggcaacata gccgaggcga aatcgccgta tcgtttcgct tgtgagctga
     6241 acttcgctca tcggcacgag atcaagagcg cgcaactggc cggtcgcgcg ttgcgtttcg
     6301 ggatcaaagg cacggatcga ttcaagggca tcgccgaaaa agtcgagccg gatggg
//
LOCUS       DATMPU010000030         8700 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_1263627,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000030 DATMPU010000000
VERSION     DATMPU010000030.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 8700)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8700)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8700
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1263627"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..604)
                     /gene="cobO"
                     /locus_tag="VNY06_00660"
     CDS             complement(<1..604)
                     /gene="cobO"
                     /locus_tag="VNY06_00660"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015928812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HWX13386.1"
                     /translation="MNGEPEAEALRHKQKMEKRKAAQDAEVASKTITQKGLLIVHTGK
                     GKGKSTAAFGLVLRALGYDWPCGVVQFIKGAWNTGERRALARYADLLSWHTMGEGFTW
                     ETQDRARDVTAAAAAWRKAQELMADPKIKLLVLDELNIALRYDYLPPADVVAALQARR
                     LDLHIVVTGRNASPEMLEAADLVTEMSPVKHHFAAGVKAQE"
     gene            complement(608..3571)
                     /locus_tag="VNY06_00665"
     CDS             complement(608..3571)
                     /locus_tag="VNY06_00665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012383937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HWX13387.1"
                     /translation="MSSKMLIDASHPEETRVVVVRGNRVEEFDFESASKRQLRGNIYL
                     AKVMRVEPSLQAAFVEYGGNRHGFLAFSEIHPDYYQIPIADRQALIEEEASAERDEEE
                     DIARPLRRSRRHRRGERNGQWAEKRRDADPSLSEAAPSEFTFDTEGIVQPEAEFEDSG
                     TFEEEPEATVHSPTPSLESAIDLVQDEMGKDSSGGEAGSSEQSAATILVDPATEPAID
                     LVSNEPLTEEQERTDPIDTNENSQILPANWAETGPDGVASDIRSEDEDETAEIEQLGG
                     DAMEEVPVRPLRPRRQYKIQEVIKRRQVLLVQVVKEERGNKGAALTTYLSLAGRYSVL
                     MPNTARGGGISRKITDPADRKRLKSIAQELEVPDGMGVIVRTAGASRTKAELKRDFEY
                     LMRMWETVRETTLQSSAPTLVYEEGSLIKRSIRDLYNKDIDEITVAGEDGYREAKEFM
                     RLLMPSHAKMVQPYRDRVPMFAKFGVETQLDAMFSNQVTLKSGGYIVINQTEALVAVD
                     VNSGRSTREHNIEDTALRTNLEAADEISRQLRLRDLAGLIVIDFIDMEEGRNNRTVER
                     RIKDALKNDRARIQIGRMSHFGLLEMSRQRMRAGVIEGSTITCPQCAGAGTVRSTASI
                     ALHVLRALEDALIKNSDYDLIVKTRTPVALYILNQKRSHLRDLEKRFGVSMTIAADDS
                     LTNTTYYTLQRGETAAGATEPPATTQLESLNVPPAEELVPTPRELQVAAASSDEESLA
                     DSDEPRESESEAYGRQRRRRRRRRGRGSDRDASGIAANAPQPPDDALEAMAKLVQLGP
                     IATSANEGATESAAPFSKNETEANHRSPRGRLRGAGHIPPIAGGEEDSGSAEVVMLEF
                     ESEPDVPASSGDPPSGENVAASGKRQRGRRAPRAGRIAGGSKAAAEPVVDPGSKDHDQ
                     GEAPAVMNEVLSRPEDMESDQSDARRSRPLDANTPLGADEARAHLEEASGPDPDPARP
                     KRSGWWQRARAGWRG"
     gene            4353..5750
                     /locus_tag="VNY06_00670"
     CDS             4353..5750
                     /locus_tag="VNY06_00670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012589769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HWX13388.1"
                     /translation="MTETATRGGLMVKSGFFPKVAGALAAMALAARALGLATLFPTLL
                     SLAPQYFGVRADTATIQTEQVQHPVASSVRLDQSTDRATLTFELNVPLEATAFVLADP
                     DRVIVDLPQTNFALDSEIGKPANLQRRKTDLVASFRFGQLEPGKSRIVIDLGAPAQLL
                     RAGCQRAEVEGDVRARLVIELAKTDKVSFRSAVQAARARLAELATVKKTRDPEPASHM
                     PIIVLDPGHGGIDRGAMARGVVEKDIVFEFAKAVAAKLESTGRFKIVMTRDEDSFVPL
                     SERVRIGRDRNASLFVSIHADTLSEAANVSGATVYTLSDRASDAEAARVAEKENQSDV
                     VAGLDQSDDNGDISGILFDLTRRETRAYSHMFAHTLVNYWRAAGRLNKNPRRSAGFRV
                     LKAPDVPSVLLELGYLSNGADSHALNSAQWRDQASGQVAEAIEAFFASREKNAPITKA
                     GPGFVPVAKIPPPER"
     gene            6114..8531
                     /locus_tag="VNY06_00675"
     CDS             6114..8531
                     /locus_tag="VNY06_00675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018408956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 1A"
                     /protein_id="HWX13389.1"
                     /translation="MRQIIRFLGFIFATGAILFVVASCAAALVVWEFGRDLPDYTTLK
                     DYEPPVMTRVHASDGSLIAEYARERRLYLPSTAIPSLVKNAFISAEDKNFYSHSGIDP
                     EGILRALFVFIQGDRHVQGASTITQQVAKNFLLTKERSFTRKFREALLAFRIEGAYSK
                     DKILELYLNEIYLGLGNYGVAAAALNYFGKSAQELTISQVAYLAALPKAPNNYHPFLK
                     RERAIERRNWVIDRMVENGFVAREDGEKAKAEPLGVNPRVLSPNAFAAGFFAEEVRRA
                     LGERYGEKKLYEGGLSVRSTLDPKMQLMARRALVDGLVRYDESHGFRGPMRHIDMSQD
                     WGIPLAEVPALGDVAPWRLAVVLDMSATGAQIGLQPHHEKSGDVERERLTGTLAAVGL
                     RWTRGAGSRASLGPGDVIYVEPIDGKPGQYRLRQVPEVSGAIVVMDPYTGRVFAMVGG
                     FSFDQSEFNRASQAMRQPGSSFKPFVYATALDNGYTPSSIILDAPIEIDQGPGLGVWR
                     PENFEGTSSGPHTLRHGVEHSINQMTVRLARDVGMPLIAEYAKRFRIYDDLPPYLAMS
                     LGSGETTLVRLTTAYSMLANGGKRIKETLIDRIQDRWGHTIYRHDERICEGCDAAEWN
                     NQSEPRLIDKREQVLDPLTAYQITSIMEGVIQRGTGQAIKEVGKHLAGKTGTTNDAKD
                     LWFVGFSPDLAVGVFMGYDQPRSLGGSAQAAHFTAPIFRDFMKMALAGKPDVPFRAPP
                     GIKLIWVDVKSGMRTSASSPGAIQEAFKPGTAPPDSYAIGDATPRAETVNHDADRLVG
                     TGTGGLY"
BASE COUNT         1740 a         2502 c         2507 g         1951 t
ORIGIN      
        1 cctcttgcgc tttcacgccg gcggcaaaat ggtgttttac gggactcatc tctgtcacaa
       61 gatcggccgc ttccagcatt tcggggctcg catttcgtcc ggtgacgaca atatgcagat
      121 ccagtctgcg ggcttgcagg gctgcaacga cgtcggccgg aggaaggtag tcatagcgca
      181 acgcgatatt gagctcatcg agcacaagaa gtttaatttt gggatcggcc ataagctctt
      241 gcgcttttct ccacgccgcc gccgctgcgg tcacatcgcg tgcgcggtct tgggtctccc
      301 aggtaaagcc ctcgcccatg gtatgccatg agagaagatc ggcgtagcgc gcaagcgccc
      361 tgcgttcgcc ggtgttccaa gcccccttga tgaactgcac gacaccgcaa ggccaatcat
      421 agcccagagc gcgcaacacg agcccgaaag ccgccgtgga tttccctttg cctttgccgg
      481 tatggacgat gagaaggcct ttttgggtta ttgttttgga ggccacctcg gcatcctgtg
      541 cggccttgcg tttctccatt ttctgtttgt ggcgtagggc ttcggcctcc ggctctccgt
      601 tcatgcttta gccgcgccag cccgctcttg ccctttgcca ccagccggag cgtttcggac
      661 gcgccggatc cgggtcgggc cctgaagcct cttccaaatg cgcacgagct tcgtccgctc
      721 cgagtggggt gttggcatcg agtggcctgg atctgcgcgc atccgactga tcactttcca
      781 tatcttcggg ccgcgaaaga acttcgttca tgactgcggg agcctcaccc tgatcgtgat
      841 ccttggaacc cggatcgact actggttcgg cggcagcctt gctgccaccg gcgatacggc
      901 ccgcacgcgg ggcgcgacgg cctcgctgcc gttttccgct tgccgcaaca ttttcgccac
      961 tgggcggatc tccactagaa gcaggcacat cgggctcaga ctcgaactcg agcatcacca
     1021 cttcggcact acccgaatct tcttctccgc cggctatcgg tgggatatgt cccgcgccac
     1081 gcaaacgccc gcgaggggac cgatgattcg cttcggtttc gtttttgctg aaaggggctg
     1141 ccgattccgt tgcaccctcg ttagccgagg tggcaattgg acccaattga acaagtttcg
     1201 ccattgcttc caaggcatca tcgggaggtt gcggcgcgtt ggcggctatt ccggaggcgt
     1261 cgcggtcact accacggccg cgccgacgtc ggcgtctgcg tctttgccgg ccataggcct
     1321 cgctttcact ctcccgtggt tcatcgctgt cggccagcga ctcctcatcg gacgaggcgg
     1381 ccgcaacctg gagttcgcgt ggggtgggca caagctcttc agccgggggc acgttgagcg
     1441 actctagctg cgtcgttgca ggcggctctg ttgcgcctgc tgcggtttcg ccgcgttgca
     1501 acgtataata ggttgtattg gtcaaactat catcggcggc gatcgtcatc gacaccccaa
     1561 accgcttctc taaatcgcgc agatgggagc gtttctgatt gagaatataa agtgcaacag
     1621 gcgtccgcgt tttaacgatg agatcgtaat ccgaattctt gatcagtgcg tcttccagcg
     1681 cgcgcaaaac atgcaaggcg atcgacgccg tcgagcgaac ggtcccggca ccggcgcatt
     1741 gggggcaggt gatggtcgag ccctctatga cccccgctcg catgcgctgg cgggacattt
     1801 ccagaagtcc aaaatgcgac atccggccga tttggatgcg ggccctgtcg ttcttgaggg
     1861 catccttgat tcggcgctcg accgtgcgat tattgcggcc ttcctccatg tcgatgaaat
     1921 cgatgacgat gaggccggcg aggtcgcgca agcgcaattg acggctgatc tcgtcggccg
     1981 cctcgagatt ggtccgcaga gcggtgtcct caatattgtg ctcgcgcgtg gacctgccgg
     2041 aattgacgtc aaccgccacc aaggcctcag tttgattgat gacgatatag ccgcccgact
     2101 tcagagttac ctggttcgaa aacatggcgt cgagctgtgt ttccacgcca aatttcgcga
     2161 acatcggtac gcgatcacga tagggctgga ccattttggc gtggctcggc atcagcaacc
     2221 gcataaactc tttggcttcg cgatagccgt cttctcctgc aaccgtgatc tcgtcgatgt
     2281 ctttgttgta taggtcgcgg atcgatcgct tgatcagtga gccctcttca tagaccagag
     2341 taggtgcgct tgattgcaaa gtggtctcgc gcaccgtttc ccacattcgc atcagatatt
     2401 cgaaatcgcg tttgagttcg gccttggtgc gcgaagcgcc cgcggtgcgg acaataactc
     2461 ccatgccgtc aggaacctcg agttcctgag cgatggattt cagtctcttt cggtcggccg
     2521 ggtcagtgat cttgcgtgag ataccgccgc cgcgcgccgt attcggcatc aatacggaat
     2581 agcggccggc cagggaaaga taggtcgtga gcgccgcgcc tttatttcct ctttcctcct
     2641 tcacgacctg cacaagcaga acttgccggc gcttgatcac ctcttgaatt ttatactgtc
     2701 tgcgcgggcg cagcgggcga actggcacct cttccatcgc atcgccgcca agctgttcaa
     2761 tttccgccgt ttcatcctcg tcctcgctgc ggatatcgct ggccacgccg tcgggcccgg
     2821 tctcagccca atttgccggc aaaatttgac tgttctcatt tgtatcaatt gggtctgttc
     2881 gctcctgctc ctctgtcagg ggttcattcg atacaaggtc aatggccggt tcggtagctg
     2941 gatcgacgag gatggttgcg gcgctttgtt ctgatgatcc cgcttccccg cctgagctat
     3001 ccttgcccat ttcatcttgg acaagatcga tcgctgattc taatgatggg gtgggagagt
     3061 gaaccgtggc ttcgggctcc tcctcgaaag ttcccgaatc ttcgaattca gcctccggct
     3121 ggacaatgcc ttccgtgtca aaagtaaatt cacttggagc ggcttcggac agtgaggggt
     3181 ccgcgtcacg ccgtttctcg gcccattgtc cgttgcgctc gccccgccgg tggcggcggc
     3241 tgcggcgcag cgggcgggcg atatcttcct cctcgtcgcg ctcagcactg gcctcttctt
     3301 caatcagagc ctgccggtcg gctatgggga tttgatagta atcagggtga atttcggaga
     3361 aggcaagaaa accgtggcgg ttcccgccat actccacgaa ggcggcctgg agggaaggct
     3421 cgacgcgcat gaccttggcg agataaatat tgccgcggag ctgtctttta ctggcggact
     3481 cgaaatcgaa ttcttcgacc cggttaccgc gaacaacgac caccctggtt tcttcggggt
     3541 gcgacgcgtc gattaacatt ttgctggaca tttgagactc gttcaggcgt gagggtcagc
     3601 gggccagggc cgccaagcaa tgtccgacag gcgaacactg aggtggggct tgaccggcgc
     3661 aaggccaata cacgcagcgt ttgggagaaa gagtggggga gcgcaatccc ctgggggata
     3721 actgatctgt tattgggcag gctgggcaaa ctgcaccatc ggtaataagg cgatggcggc
     3781 tctgggcttt ttggcgctcg acccgcataa actggagaac tctactttcg catactcggc
     3841 gggcgactaa ccaccaagcg ggggcaggtc ctcggctaat atgctcggcg agggcgtaat
     3901 gcctcttttc gggacaactc atgtcctcgt tacctgttac cggcattgct gggaaagacg
     3961 ccaaccaact atcatccagg cagacaccgc ttcagccaag cctaagtttg tacgcgccag
     4021 aaccgatttg ttcttgttta ggcgctcgca acacgacgcg gctagccaat atacaaatgt
     4081 cctaatcctc aacgcggaaa tggggcgcaa caccagaacc acttcggccg aggacggaaa
     4141 tttccgagcc taaccaattt atcactattc atatcggtat tgttggctgt ttggcaagtt
     4201 tccttggtgc tttgtctcgc cggttccggt tttcgctgtg ggtttcttcc ttgatcacaa
     4261 ctcgttaacc tagcccgctc taaagactaa aggtgaccga ggaaggacga aaacgccgct
     4321 gccgcaacat aaattccaga gcggccgctg cagtgactga gacggcgacc cgaggcggat
     4381 tgatggtcaa gagcggtttt tttcccaaag tcgctggcgc gttggccgcc atggccctcg
     4441 ctgctagggc gctgggactc gccacattat ttccaacgct gctatcttta gctccacaat
     4501 acttcggtgt tagggctgat accgcgacca tccaaacaga acaggtgcag catccggttg
     4561 cgagttcggt ccggctcgat caaagcaccg atcgggccac gcttacgttc gaacttaacg
     4621 tgcccctcga agcaacagcc tttgttcttg ccgatcctga ccgggtcatc gtcgatttgc
     4681 cccaaaccaa tttcgcgctc gattcggaga ttggcaagcc ggcaaatctg cagcggcgaa
     4741 agactgatct cgtcgcatcc tttcgatttg gccaactcga accgggaaag tcacgcattg
     4801 tgatcgatct tggtgcgcca gcccagctgc tgcgggcagg ttgccaaagg gcggaagtag
     4861 aaggtgatgt cagagctcgc ctggtaattg agctagccaa gaccgacaaa gtgagctttc
     4921 ggagtgcggt ccaagccgcc cgtgcgcgat tggcggagct cgcgacagtc aagaagaccc
     4981 gtgatccaga gccagcttcg cacatgccga tcatcgtgct cgatcccggc cacggcggaa
     5041 tcgatagagg tgccatggcg agaggtgtcg tcgaaaagga catcgtgttc gagttcgcta
     5101 aggccgtagc ggctaagcta gagtcaacag gccgtttcaa gatcgttatg acgcgcgacg
     5161 aggacagttt tgtgcccttg tccgagcgcg ttcgaatcgg acgtgaccgc aacgcgtctt
     5221 tgtttgtttc gattcatgcc gatacattgt ccgaagcggc caatgtttcc ggtgcgaccg
     5281 tctacacgct ctcagatcgc gcctcggacg cagaagcagc ccgcgtggcc gaaaaagaaa
     5341 atcagtccga cgtcgtggct ggcttagatc aatccgacga caatggcgat atttccggta
     5401 tcttgttcga tttaacgcgg cgcgagaccc gtgcgtacag tcacatgttc gcccatacct
     5461 tggttaatta ctggcgggcc gcgggacggc tcaacaaaaa tccgcggcgt tcggccggtt
     5521 ttcgtgtgct gaaggccccg gacgttcctt ccgttttgct ggagctcggc tatctttcca
     5581 acggtgcgga tagccacgcg ctgaattcgg cacagtggcg agatcaggcg tccggccaag
     5641 tcgcggaggc gattgaagca ttcttcgcgt cgcgggagaa aaatgcgcct attactaagg
     5701 ccggccccgg ttttgtaccc gtagcaaaaa ttccgccccc cgaaaggtag acttaagatt
     5761 tcaccaaagc cgcgaagtta gcctccggca agaattgcac cctcgaatct ccctaggacg
     5821 aaacggtgct gtgcgtgcaa ttttgcaatc ttcctccgcg gggcgctgac ctggccgaca
     5881 aaatatcaac acaaaagcgc cgcacggagg aggtgacatt tctgcgggat gtgtcctgga
     5941 gagataaatt cggggcggac agctatcgga attggagaac cgaatagcca ttatttggct
     6001 tggattggcg cgccaatatg agcgcaacag ctttcggaca ccggcaacgc tgcaggcagg
     6061 actcaaattg atttcgttcg gcatcagttc cattttcatg aggacctgaa cgtatgaggc
     6121 aaattatccg gttcctcgga ttcatttttg caacgggcgc gatccttttc gtcgtcgcgt
     6181 cgtgcgcggc cgcgctcgtg gtttgggaat tcgggcggga tcttccagac tatacgacac
     6241 tcaaggatta cgaaccacct gtcatgacaa gagtccatgc gagcgatggt tcactaatcg
     6301 ctgagtacgc gcgcgagcgg cgcctttacc tcccgagcac cgcaattcca tcgctcgtca
     6361 aaaacgcctt catttcggcc gaggacaaga acttctattc ccattccggt attgatccag
     6421 agggcatcct tcgagcgctc ttcgtcttta ttcaaggcga tagacatgtc caaggcgcat
     6481 cgacgatcac gcaacaggtc gccaagaact ttctcttgac gaaggagcga tcctttacgc
     6541 ggaaattccg cgaggcgctg ctcgcgtttc gtatcgaggg tgcctattcc aaggacaaaa
     6601 ttctcgagct ttacctgaat gaaatctacc ttggtcttgg aaactacggc gtggctgccg
     6661 cggcgctcaa ttatttcggc aagtccgcgc aggagctcac tatatcccag gttgcctatc
     6721 tcgcagctct gccaaaggca cccaacaatt atcatccttt cctaaagcgg gagcgcgcga
     6781 tcgaacgccg caactgggtt atcgaccgta tggtcgaaaa cggcttcgtc gcgcgcgagg
     6841 atggcgagaa agccaaggcc gagccacttg gcgtcaatcc tcgggttctt tcgccaaacg
     6901 ccttcgccgc tggcttcttt gccgaagaag ttcggcgcgc gctcggcgag cgttacgggg
     6961 agaagaagct ctatgagggt ggtctctctg tacgtagtac cctcgatccc aaaatgcagc
     7021 tgatggcaag gcgcgctctg gttgacggcc tcgtccgcta tgacgaatcg catggttttc
     7081 gtggcccaat gcgccacatc gacatgagcc aggattgggg gattcctctt gccgaggttc
     7141 cggctttggg cgatgtggcg ccttggcggc tcgctgtagt tctcgatatg agcgcaacgg
     7201 gcgcgcaaat tggcctgcag cctcatcatg aaaaatctgg cgatgtcgag cgcgagcggc
     7261 taaccggcac gcttgccgcg gtcggacttc gttggacccg tggcgctggc tcgcgagctt
     7321 cgcttggacc gggcgatgtg atctatgtcg agccaataga cgggaaaccc ggtcagtatc
     7381 gactgcgcca ggttcccgaa gtcagcggcg caatcgtcgt aatggacccc tataccggca
     7441 gggtgttcgc catggtgggt ggcttctcct tcgatcagtc cgagttcaac cgtgcaagcc
     7501 aggcaatgcg gcagccgggg tcctccttta agccttttgt ttatgccacc gcgctcgaca
     7561 atggctatac gccatcctct atcattctcg acgctcctat cgagatcgat caagggcccg
     7621 gacttggggt gtggcgtccg gaaaatttcg agggcacgtc cagcggcccc cacactttgc
     7681 gccacggcgt tgagcattcg atcaaccaaa tgacggtccg gcttgcgcgt gatgtcggca
     7741 tgccgctcat cgccgaatat gccaagcggt ttcgcattta tgacgatctg ccgccctatc
     7801 tcgcgatgtc gcttggctcc ggcgaaacga cgctggtgcg cctgaccacc gcctattcga
     7861 tgctcgccaa tggcggcaag agaattaaag agacattgat cgaccgcatt caggaccgat
     7921 ggggtcatac aatctaccgg catgacgagc gaatttgcga gggatgcgac gcggcggaat
     7981 ggaataacca aagcgaaccg agactaatcg acaaacgcga gcaagttctc gatccgctga
     8041 ccgcatatca aatcacctcg attatggagg gcgtcattca acgcggaaca gggcaggcga
     8101 tcaaggaagt tgggaagcac ctcgcgggca agaccgggac gaccaacgac gccaaggacc
     8161 tttggttcgt cggattctcg cccgacctcg cggtcggggt cttcatgggc tatgatcagc
     8221 cgcgctctct cggaggatcg gcgcaggccg cgcattttac ggcgcccatt ttccgggatt
     8281 ttatgaaaat ggcccttgcc ggtaaacctg acgtcccgtt ccgtgcaccg cctggcatca
     8341 agctaatctg ggtcgacgtg aaatcgggaa tgaggacgag cgcaagttct ccgggagcta
     8401 tccaggaggc gtttaagcct ggcacggcgc cgcccgactc ttatgccatt ggcgacgcga
     8461 caccgcgggc cgagacggtt aatcacgacg ctgaccgcct ggtcgggacc ggaaccggcg
     8521 ggctatatta gttttaaagg gtgctttgcc ggagacgtaa tctcggggaa agcctttatc
     8581 ttaaaaaagt aaatcatgga gttaacgtat cagctcgcaa gccagagaga ggcagcgggc
     8641 ctaagggcgc tatcgccgcc gggcagatcg ggataggaat gtgcgaccag tcaaagaggt
//
LOCUS       DATMPU010000031         3219 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_4052330,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000031 DATMPU010000000
VERSION     DATMPU010000031.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 3219)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3219)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3219
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4052330"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..236)
                     /locus_tag="VNY06_00680"
     CDS             complement(<1..236)
                     /locus_tag="VNY06_00680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX13390.1"
                     /translation="MLKNLIYAVGLTLSLAVASGRAEVPPQPSTAPAEPAKLTGGDDL
                     GKLQAALAQIRESLARHNLTAAELQALREQIDPLS"
     gene            459..562
                     /locus_tag="VNY06_00685"
                     /pseudo
     CDS             459..562
                     /locus_tag="VNY06_00685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_044551230.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            715..939
                     /locus_tag="VNY06_00690"
     CDS             715..939
                     /locus_tag="VNY06_00690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX13391.1"
                     /translation="MNQFVKYRQTAAECCEAARILSDPREKAKMLAMAQSWILLADQA
                     ERNSHLKAATAGLTGHGNRNPALFHIFRQG"
     gene            complement(1556..2092)
                     /locus_tag="VNY06_00695"
     CDS             complement(1556..2092)
                     /locus_tag="VNY06_00695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003598253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HWX13392.1"
                     /translation="MGFFSKDIETIDDLIVHTLQDIDYAEQRILQTLPTMIEKATNLQ
                     LRSAFEHHLAETRNHLHRVERVFEMHGVERETVHCPAIDGIIQEADEVSGEVDDKQVL
                     DATLIAAADAALIAAAQAIKHYEISRYGTLIAWVKQLGHNDCASVLERNLEEEKAADQ
                     KLTHIAESQVNVQASLSA"
     gene            complement(2199..2369)
                     /locus_tag="VNY06_00700"
     CDS             complement(2199..2369)
                     /locus_tag="VNY06_00700"
                     /inference="COORDINATES: protein motif:HMM:NF022150.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KGG domain-containing protein"
                     /protein_id="HWX13393.1"
                     /translation="MARVFPMKSEAFHETASSPRRSAAKAVTPSGGNSLNDPERASRA
                     GHRGGEASHGNR"
BASE COUNT          648 a          920 c          907 g          744 t
ORIGIN      
        1 gaaagcggat cgatttgctc gcgcagggct tggagttctg ccgccgtcag attgtggcgt
       61 gcgaggcttt cccttatttg cgccagggca gcctgaagct tgccgagatc gtcaccgcct
      121 gtcaacttgg cgggctccgc cggtgccgtg gaaggttgtg gcggcacctc cgcccttccc
      181 gacgcgaccg caagtgacaa ggtgagtcca accgcgtaga tgagattctt cagcaaagcc
      241 ggatccctat ctgtttgata acagagaatt tctctggttc aatttaccta aagcagaaaa
      301 gttgaagctc gcaagtcatt gatttttcga tacctgattg taggaatttg cttcgaaaag
      361 gcaactttgc cccacaaccc ctaccgcgac cctccactcc tcgatggttg cgatgagcca
      421 gtttttggaa cttgacgtcc tgcgcctctg atattgttgg tggcgcgcat tattgggcgc
      481 gcgtgaactc aaggccatgg gtcacgaagt gctgctggtg ccgcccgctt acaccaagcg
      541 ctatttcgag cgcggcaaga acgcgtcaca taaaggcaac ggttcgctaa aaaattaagc
      601 tcatggctgg tttcgaattg gacaatggcc ggctttcata atcgggtaag gacttccgct
      661 cacgtctgag cgcctggcaa ttaatacgtc aatttttact agggtattcg cccgatgaac
      721 cagttcgtga aataccggca gaccgccgct gagtgctgcg aggcggcccg gattctctcc
      781 gatcctcgtg agaaggcaaa aatgcttgcg atggcgcagt catggatatt gctggccgac
      841 caagccgagc gaaacagcca tcttaaggcc gccactgctg gattgacggg gcatggaaac
      901 cgaaacccgg ccttattcca tattttccgg caaggctaag aacccatagc gcccccgtga
      961 cacaaacagc tcgcgaagct catgggtcga tatccttacc ggcccaggga caggtcagga
     1021 tcgagataac ctcgagcatg gcattgtggc gatcgccgaa gcctgacgtc ttgacggccg
     1081 cgaccctcga ggccgcttag ccgatgtgct tgtctggccg atgacgctcc gcatcacccc
     1141 agcgatggtg gctggcctga tcggctttgg ggaatttgtg aaaatgagaa ggtatggact
     1201 tcccgcaatt ttcgcatctc aagtggtaaa tcctcaactt ggatgagcct cgatcacagc
     1261 taacttcaac acactccggc agtcaggaca tagatctatc agttgtgctg tgcgcgtcac
     1321 attacgctgc atgatctttc tcccggtgga aaggggctga aactgaattg ggcaccaaag
     1381 gttccgaatt ccatggacgg cgcacgaggc ttcgctattg acgatgagga tgccggttga
     1441 aggggtgcga cgcctatgat gatggtctgt cgtcgcgctc atctgcgtga ggcttcgccg
     1501 cggctgcatt ctggttccgc tcgcaacctt gggcgtactt ttgcgtcatg cggaatcatg
     1561 ccgaaagcga ggcttgaaca ttgacttggg attccgcgat gtgtgtcagc ttttggtcag
     1621 ctgccttttc ttcctcgagg ttcctttcca ggacgcttgc acagtcgtta tgcccgagct
     1681 gcttgaccca ggcgatgagc gtgccgtagc ggctgatttc ataatgcttg atagcctgcg
     1741 ccgcagcgat tagggccgcg tcagccgcag cgatcagggt cgcgtccaga acttgtttgt
     1801 catctacttc gccggagact tcgtcggctt cctgaatgat gccatcgatg gccgggcagt
     1861 ggactgtctc acgttcgacg ccatgcattt cgaacacgcg ctcgacccgg tgcaggtgat
     1921 tgcgcgtttc ggcaagatga tgctcgaaag ccgacctcaa ttgcagattt gtggcctttt
     1981 cgatcatggt cggcaaagtc tgcaaaatcc gttgttccgc gtagtcgatg tcctggagcg
     2041 tgtggacgat caaatcatcg atcgtttcga tatctttgga gaagaagccc atgactttca
     2101 ccttgtcgac ttgaagttga tttgaaccgg agcacgcatc cacaatgcta aaagcacgcc
     2161 tccttcaagc ggaagcaaga gccggagtcc agcggcggtt agcggttacc gtgcgatgct
     2221 tctccgccgc gatggccggc tctgctcgcg cgttcaggat cgttaagcga attgccgccc
     2281 gacggagtga ccgcctttgc ggccgacctc cgcggcgagc tcgcagtctc gtggaaagct
     2341 tcgcttttca tcgggaacac tcttgccatc cttgcggcca gctgcggacg tgcgctcgtg
     2401 atccttgaga aactgcgctc ctcgtcgggc acgctttcac cacctctgcg ggcaatttcg
     2461 cttgtcttag tccatggaag caaagccacg attcgaggtt gtttaacgct gcaaaaaagc
     2521 aaatctcgat gaagtggcgc atacgccgga cggcaccagg ctgatctcga atttcgttca
     2581 caagatcgcc gggctcaagc cagattggac ccggcaaaat tgcgctgccc ggcccgtcag
     2641 taatacccgt ccctgcctca gcgtccgatt ggttcgctcc tgtttccgtg cagcgcagag
     2701 tgaccgcgat ccgatctgcg tggaccatga ccctttcgac atgctggctg atgaattcgt
     2761 ggtcggggat atcattgcct gcgggcttcc atggcacgtc cctatgcaag acggacctag
     2821 gccaaacccg gggtgtgctc gcgacgggcc tacagggacc ccctcagtga ctccgcgact
     2881 ggcccgctgg caggctcttc ggatttcgcc ttgctgcctt tcatcgaacg cgcggccata
     2941 ggacactcgc tgaggtttgc cctggaattt ggtaggttcg tctgccaaac tggcggcatg
     3001 aacagcggtg aaaaactcgc catcctggag gttgcgcctg gaaagcggtc aaggcgcccg
     3061 aatgacgccg gtgcgccgga ggaaacccac gctcgtggcg ctgacccagc catccgctgt
     3121 ttcattgccc ccatccaggc tacgcccgct gtctttggtt catcagcccc cgcgcgcgct
     3181 ccataccctg atgcatagca agcgtaaatt gcttgaaga
//
LOCUS       DATMPU010000032         2680 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_3440093,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000032 DATMPU010000000
VERSION     DATMPU010000032.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 2680)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2680)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2680
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3440093"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            90..707
                     /gene="cobO"
                     /locus_tag="VNY06_00705"
     CDS             90..707
                     /gene="cobO"
                     /locus_tag="VNY06_00705"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015599117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HWX13394.1"
                     /translation="MNEEPEAEALRHKQKMEKRKAAQDAEVASKTIIQKGLLIVHTGK
                     GKGKSTAAFGLVLRALGYDWPCGVVQFIKGAWSTGERKALARYADLLSWHTMGEGFTW
                     ETQDRARDVAAAAAAWRKAQELMADPKIKLLVLDELNIALRYDYLPVADVVAALQARR
                     LDLHIVVTGRNASPEMLEAADLVTEMSPVKHHFAAGVKAQEGIEF"
     gene            734..2176
                     /locus_tag="VNY06_00710"
     CDS             734..2176
                     /locus_tag="VNY06_00710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020095590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobyric acid synthase"
                     /protein_id="HWX13395.1"
                     /translation="MLQGTNSGAGKSLIVAGLCRAYRNRGLEVRPFKPQNMSNNAAVT
                     PDGGEIGRAQATQARACGIAPSVDMNPVLLKPQSGDGAQIVVRGRVQGRARAREYQDL
                     KRKLLPEVLESFERLRARSSLVLVEGAGSAAEINLRKNDIANMGFARAADIPVILAGD
                     IDRGGVIAQIVGTKAVLDPRDARMIEGFVINKFRGDPALFAGGIQLIEEVTGWTSFGL
                     IPYCAEAARLPEEDSLGLSALRPQGDGPVMIAVPVLPGIANFDDLDPFYLEREVRLVM
                     VGSGETLPAEASLVILPGSKTTIADLAVFRREGWDIDLFAHLRRGGHVLGLCGGYQML
                     GKIIRDPLGIDGTPGEAQGLGLLDIETHLEGGKVLLPVQGTGAHDGVPFSGYEMHVGQ
                     TFGSDRTRPLLRFVDGRNDGAVSKDGRVSGCYVHGLFASAAQRSALLARLGGKGSGVS
                     YEESLDVALDAIASHLEKHIDLDRLLTLAR"
     gene            complement(2160..>2680)
                     /locus_tag="VNY06_00715"
     CDS             complement(2160..>2680)
                     /locus_tag="VNY06_00715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019401238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0048472 - threonine-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cobalamin biosynthesis protein"
                     /protein_id="HWX13396.1"
                     /translation="SLAENYSDGVVAPAFFLAIGGLAGAACYKTINTADSMIGHRTER
                     YAAFGFASAKLDDFVNYCPARLSALWIIIASLFTKGASFKNAWRTVRHDARHHPSPNA
                     GWPEAAFAGALGLRIGGPRIYGDVKVNAAWIGTGNRASINDIIIALALYRRACAVHWL
                     VLAMMALAISLA"
BASE COUNT          556 a          769 c          832 g          523 t
ORIGIN      
        1 aagaggcttc agggcccgac ccggatccgg cgcgtccgaa acgctccggc tggtggcaaa
       61 gggcaagagc gggctggcgc ggctaaagca tgaacgaaga gccggaggcc gaagccctac
      121 gccacaaaca gaaaatggag aaacgcaagg ccgcacagga tgccgaggtg gcctccaaaa
      181 caataatcca aaaaggcctt ctcatcgtcc ataccggcaa aggcaaaggg aaatccacgg
      241 cggctttcgg gctcgtgttg cgcgctctgg gctatgattg gccttgcggt gtcgtgcagt
      301 tcatcaaggg ggcttggagc accggcgaac gcaaggcgct tgcacgctac gccgatcttc
      361 tctcatggca taccatgggc gagggcttta cctgggagac ccaagaccgc gcacgcgatg
      421 tggccgcagc ggcggcggcg tggagaaagg cgcaagagct tatggccgat cccaaaatta
      481 aacttcttgt gctcgatgag ctcaatatcg cgttgcgcta tgattacctc cctgtggccg
      541 acgtcgttgc agccctgcaa gcccgcagac tggatctcca tattgtcgtc accggacgga
      601 atgcgagccc cgaaatgctg gaagcggccg atctcgtaac agagatgagt cccgtaaaac
      661 accattttgc cgccggcgtg aaagcgcaag aggggatcga gttctgagga ccgcacggcg
      721 ggcaaagagt ttaatgctgc aaggcacaaa ctcaggtgcc ggaaagtcac tcattgtcgc
      781 cggtctttgc cgagcctacc gcaatcgcgg gctcgaagtt cgcccgttta agccgcagaa
      841 catgtccaac aatgccgccg tgacgccgga tggtggagag atcggccgcg ctcaggcaac
      901 ccaggcgcgg gcttgcggga tcgctccgag cgtcgacatg aaccctgtgc ttcttaagcc
      961 tcagagcggc gatggcgccc agatcgttgt gcggggccgc gtacaaggcc gggcgcgcgc
     1021 ccgggaatac caagacctga aacgcaaact tctgcctgaa gtactcgaaa gtttcgaaag
     1081 acttcgcgcg cgttccagcc tcgtccttgt cgaaggcgca gggagcgcag cagaaatcaa
     1141 tctgcgcaaa aacgacattg ccaatatggg tttcgcgcgc gccgcagaca tccccgtaat
     1201 tttggcgggc gatatcgatc gcggcggtgt cattgcccaa atcgtcggca ccaaagcggt
     1261 acttgatccc agggacgcca gaatgatcga gggttttgtc atcaacaagt ttcgcggcga
     1321 tccggcccta ttcgcaggcg ggattcaact aatcgaggaa gtgaccggct ggacatcttt
     1381 cgggcttatt ccttattgtg ccgaagcagc gcggttgcct gaggaggact cgctcgggct
     1441 ttccgcgctt cggccgcaag gtgatgggcc ggtcatgatt gctgtcccgg ttttgcccgg
     1501 catcgccaat ttcgacgatc tcgacccttt ttatttggag cgcgaggttc ggcttgtgat
     1561 ggtcggaagc ggcgagacgc tgccggccga ggcatcgctc gttatcctgc ccggctcgaa
     1621 aacgacgatc gccgatctcg cggtcttccg gcgcgagggc tgggatattg acctcttcgc
     1681 ccatctgcgc cgcggtggcc atgtattggg cctttgcggc ggatatcaga tgctcggaaa
     1741 gattatccgc gatcctttgg gcattgacgg cactccaggc gaggcgcagg gccttggctt
     1801 actcgacatc gaaacgcacc ttgagggcgg caaagtgctt ttgccggtgc aaggcacggg
     1861 ggcgcacgac ggcgttccat tcagtggcta tgaaatgcat gttggccaga catttggatc
     1921 tgaccgcacg cggcccttgc tgcgtttcgt cgatggtcgc aacgatggcg cggtatcgaa
     1981 agacggccgg gtgagcggct gctatgtgca cggccttttc gcctcagcag cgcagcgcag
     2041 cgctctcctc gcccgccttg ggggcaaggg atctggcgtt tcatatgagg agtcgctcga
     2101 cgtggcactc gacgccatcg cctcccatct cgaaaagcat atcgaccttg accgtctcct
     2161 cacgctagcg agatagcgag cgccatcatc gccaggacga gccaatggac ggcacatgcc
     2221 cgtcgataga gcgccagcgc tattattatg tcattgatgg aagcgcggtt gccagttcct
     2281 atccaggcag cgttgacctt gacatcgccg tagatgcgcg gtcctccgat ccgcaagccg
     2341 agcgcacccg caaatgctgc ctctggccag ccggcgttgg gagatggatg atggcgcgcg
     2401 tcgtgccgca ccgtgcgcca tgcatttttg aatgaggctc cttttgtgaa caggctggca
     2461 atgatgatcc acagcgccga aagtctggca ggacaatagt tgacaaaatc gtcgagtttt
     2521 gcggaggcga agccaaaagc ggcataacgc tctgtgcggt gaccgatcat gctgtcggcg
     2581 gtgttgatgg ttttgtagca ggcggcacca gcaaggccgc cgattgcaag aaagaaggcc
     2641 ggcgccacga cgccatcaga ataattctcg gccaggcttt
//
LOCUS       DATMPU010000033         5943 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_2503750,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000033 DATMPU010000000
VERSION     DATMPU010000033.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 5943)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5943)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5943
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2503750"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..349
                     /locus_tag="VNY06_00720"
     CDS             <1..349
                     /locus_tag="VNY06_00720"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009452437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HWX13397.1"
                     /translation="CMPPGANGKNEPLRVIPSQVDRLERKIDELNAELGPLRSFVLPG
                     GSKAAAALHLARTVTRRAERLLVALANTPGEEVGAPALKYVNRLSDFLFVAARYMNSK
                     GEADILWVPGQNR"
     gene            540..1289
                     /locus_tag="VNY06_00725"
     CDS             540..1289
                     /locus_tag="VNY06_00725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006936816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HWX13398.1"
                     /translation="MKIMIPVKRVVDFNVKIRVKPDGSGVDLANVKMSINPFDEIAVE
                     EALRLKESGNASEVIVVSVGPAEATETIRSGLSMGADRGILVKVAEPIEPLTVAKLLK
                     AIALAEEPRLIILGKQAIDDDCNQTGQMLAALLGWGQGTFASKVILGDGHVDVTREVD
                     GGLQTVSLKLPAVVTTDLRLNEPRYASLPNIMKAKKKVIEEKTPEDLGVDIAPSLRVL
                     KTVAPTGRKAGVKLASAAELVARLKEEAGVI"
     gene            1289..2233
                     /locus_tag="VNY06_00730"
     CDS             1289..2233
                     /locus_tag="VNY06_00730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018453240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HWX13399.1"
                     /translation="MPVLLLAEISNSTLNESTAKALTAAKGLGKPIHILVAGENAEPA
                     AQAAAKLDGVEKILLADDKAYAHQLAEPLAALLVSLASNYSALVAAANSTGKNVMPRA
                     AALLDVMQISDIVKVVAPDVFERPIYAGNAIATVQSTDNRKVITVRTAAFPASGVGGP
                     APIERIATAAAPELSSFKDEILAKSERPELSAAKIIISGGRGLRNAENFKTLIEPIAD
                     KLGAAIGASRAAVDAGYAPNDWQVGQTGKIVAPDLYIAVGISGAIQHLAGMKDSKVIV
                     AVNKDEEAPIFQIADYGLVGDLFELLPKLNEELAKAGR"
     gene            2655..3530
                     /locus_tag="VNY06_00735"
     CDS             2655..3530
                     /locus_tag="VNY06_00735"
                     /EC_number="1.1.1.157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012590051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyryl-CoA dehydrogenase"
                     /protein_id="HWX13400.1"
                     /translation="MDISKIGIIGAGQMGTGIAQVCAQTGIEVALNDISEARINAGLA
                     TIAGNLARHVERNQLEQKAGDAALSLIKPARDFEAFGDCDLVIEAATENEEVKRKIFA
                     KLCPSLKPTALLATNTSSISITRLASITDRPERFIGIHFMNPVPRMQLVELIRGIVTD
                     NATFESAKALVRRLGKTFTVSEDFPAFIVNRILLPMINEAIYTLYEGVGSVESIDTAM
                     RLGANHPMGPLQLADFIGLDTVLSVMQLLHDGMADPKYRPCPLLVKYVEAGWLGRKTQ
                     RGFYDYRSGTPIPTR"
     gene            3711..4280
                     /locus_tag="VNY06_00740"
     CDS             3711..4280
                     /locus_tag="VNY06_00740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012386012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HWX13401.1"
                     /translation="MTIHSQPRVSPEGCPALVLNADFRPLSYYPLSLWSWQNSIKAVF
                     LDRVNIVSSYDKTVRSPSFEMRLPSVISLKSYVKASRYPAFTRFNVFLRDRFSCQYCG
                     SREDLTFDHLTPRSKGGTTTWKNVVAACSSCNLRKGDQLPAEARMWPSRMPYQPSVND
                     LHQNGRRFPPNYLHESWLDYLYWDSVLEP"
     gene            complement(4305..5465)
                     /gene="argE"
                     /locus_tag="VNY06_00745"
     CDS             complement(4305..5465)
                     /gene="argE"
                     /locus_tag="VNY06_00745"
                     /EC_number="3.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018408111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008777 - acetylornithine deacetylase
                     activity [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine deacetylase"
                     /protein_id="HWX13402.1"
                     /translation="MDERLDATLSILERLINFDTESSKSNLALVSFVEDYFKALGVSY
                     VKIPNAAGDKAALFATIGENCDGGIVLSGHTDVVPVAGQAWTSDPFKLRRENGRLYGR
                     GTCDMKGFDAVCLAMIPEFKNTRLTRPIHILLSYDEEITCRGPLDTIAQFGTGLPCPG
                     AVLVGEPTLMQVADAHKGVGGYATVVHGHEAHSSKPALGASAIEAACDLVTELYRFGA
                     KLMAEGDPSRRFDPPASTIHVGMIQGGTARNIMAKLCTFHWEFRALPDVAQNLALRHL
                     ENYAARSVVPKLTRYPKHASIETETLVEVPGLKPVSGSIAESLALKAGRSNRTISVPY
                     ASEAGQFQLADLPAVVCGPGSIDQAHQPDEYIEIAQVESCIAFMRRLTAELS"
     gene            complement(5546..5938)
                     /gene="apaG"
                     /locus_tag="VNY06_00750"
     CDS             complement(5546..5938)
                     /gene="apaG"
                     /locus_tag="VNY06_00750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015689150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HWX13403.1"
                     /translation="MYTIVTHDIQITVLPEFVPERSDADEASYWWAYTVEIANRGEVT
                     VQLTARHWKITDGNGRLEEVKGAGVVGEQPILKPGETFRYTSGTNMSTPSGIMTGTYK
                     MVNEKGESFEAEIPVFSLDSPYARRVLN"
BASE COUNT         1308 a         1700 c         1668 g         1267 t
ORIGIN      
        1 ttgcatgccg cccggtgcga atggcaaaaa cgagccgctg cgcgtcattc cgtcgcaagt
       61 cgaccggctg gaacgcaaaa ttgacgagct caacgcggaa ctcgggccct tgcgttcctt
      121 cgttctgcca ggcggctcga aggccgccgc cgccttacac cttgcgcgca ctgtgacgcg
      181 ccgggccgaa cgcttgctcg tcgccttggc caatacgccc ggcgaagaag ttggcgcgcc
      241 tgcgctcaaa tatgtcaatc ggctttctga tttcctgttc gtcgccgcgc gctatatgaa
      301 cagcaaggga gaggccgata tactgtgggt gccagggcag aatcgttgag aatctggaaa
      361 tgcggattaa ggacgtcatc tgagacaatc tactcttcgg ttgcactcat cgagtcagtg
      421 ggctgggtcc gcgccgaccc atcgatcctt agcctttctg accctgcgcc ggttgcgttg
      481 acttggccag gaacgctatt acccggattg gcctctcgcc ggcagctcgg agattgccca
      541 tgaaaatcat gatccccgta aaacgggtcg tcgatttcaa tgttaagatc cgagtgaaac
      601 cggacggctc cggcgtcgat ctcgccaatg tcaaaatgtc gataaaccct tttgacgaaa
      661 ttgccgtcga ggaggcgctg cggctcaaag agtccgggaa tgcgagcgaa gtcattgtcg
      721 tctcagtcgg tccggctgag gccacagaga cgatccggag cggtctttcg atgggtgcag
      781 atcgcgggat tttggttaag gtggccgagc ccatcgagcc gctcaccgtt gccaagcttt
      841 tgaaggcaat agctctcgcc gaggagccgc ggctcattat ccttggcaag caagcaattg
      901 acgatgattg caatcagacc ggacagatgc tcgccgcgct cttgggatgg ggacagggaa
      961 cctttgcctc aaaagtaatt ctaggcgatg gccatgtgga tgtcacccgc gaggtggacg
     1021 gcggattgca gaccgtgagc ctcaagcttc ccgccgtcgt aacaactgat ctacgcctta
     1081 acgaaccgcg ctacgcctct cttccaaaca tcatgaaggc caaaaagaag gtcattgaag
     1141 agaagacccc cgaagatcta ggcgtcgata tcgcgccgag tctccgagtc ttgaaaacgg
     1201 tcgcgccgac ggggcgcaag gccggggtca aactcgcctc cgcagccgaa cttgtcgcaa
     1261 ggctcaagga agaagcaggg gtgatctgat gccggtgctg ctcctcgccg agatttcaaa
     1321 cagcacgctt aacgaatcga ccgccaaggc tctgacagct gcgaagggcc tcggcaaacc
     1381 catacatatc ctcgtcgccg gggagaacgc cgagcccgcc gcgcaagctg cagccaaact
     1441 ggacggcgtc gaaaagattc tgctggcaga cgacaaagcc tatgcgcatc aactggccga
     1501 gccgctggcc gcgctccttg tctctttggc aagcaattat tcggcgctcg ttgccgcagc
     1561 gaattcgacc ggcaagaatg tcatgccaag ggccgcggcg ctgcttgatg tgatgcagat
     1621 ttccgacatt gtgaaggtcg ttgcacccga cgtctttgaa cggccgatct atgctggcaa
     1681 tgcgattgcg acggtgcagt cgacggataa cagaaaagtc atcacggttc ggactgccgc
     1741 atttccggca agcggggtcg gcgggccggc tcccatcgag agaattgcca cggctgctgc
     1801 gccggaacta tcgagcttca aggatgaaat ccttgccaaa tccgaacgcc cggagctttc
     1861 cgcggcaaag attatcatat ctggcggacg cggcctgcgg aacgcggaaa actttaagac
     1921 cttgatcgag cccatcgccg ataagcttgg cgccgccata ggagcatcgc gcgcggcagt
     1981 cgatgcgggt tacgccccaa atgactggca ggtcggccag actggaaaga tcgtcgcacc
     2041 cgacctctac attgccgtag gtatatccgg cgcgatccag caccttgccg ggatgaagga
     2101 ctccaaagtg atcgtcgctg ttaacaagga cgaagaggct ccaatatttc agatcgccga
     2161 ttacggtctc gtcggcgatc tctttgaact tttgccaaag ctgaacgagg agcttgccaa
     2221 ggcgggccgt tagagcgcgt cgatccaaaa gctcgaaccg gttttgcctc cggacacgcg
     2281 aagagacgaa gaacgagacc gataggatga ttcaaagaat agtcctctcg ctcgaggcgc
     2341 tttgctgaaa tttagagact tctgcacgcg tttgatggaa aaattactac ccgttcggag
     2401 tagctcccgc tgtgctgctg aacgcccacc ctcgaggctt tgcaagtcat cgggcctgtt
     2461 cacgcgtgag ttttgacgaa agccgttcgg cattgaacca atcgttactt cgacagctaa
     2521 atcaactcag tagaaatatc ttcctctttc gtttaaaaat ctgctgggat cgcgcgaaca
     2581 tgcctcggcg agtgcaagcg tgaagtcaat ccgacccggg ccttcgagcc tgtttgccgc
     2641 cgcggacgaa tgagatggac ataagcaaaa tcggaataat aggagccggc cagatgggca
     2701 cgggaatagc ccaggtctgc gcccaaactg gtatcgaagt ggctctcaat gatatatccg
     2761 aagcgcgcat caacgccggt cttgccacca tagctggcaa cctggcacgc catgtcgaac
     2821 gaaaccagtt ggagcaaaag gcgggcgacg cggcgttgag cctgatcaag cccgcgcgcg
     2881 acttcgaggc ttttggggac tgcgatctcg tcatcgaagc cgccactgaa aatgaggagg
     2941 tcaaacggaa gatttttgca aaactttgcc cctcattgaa gccaacggca ctcttggcca
     3001 cgaacacatc atcgatttcg atcacgagac tcgcttcgat cacggatagg cccgaacggt
     3061 tcatcggcat tcatttcatg aatcctgttc cgcgcatgca gctcgtcgaa ctgattcgcg
     3121 gcatcgtgac cgataacgcc acattcgaat ctgccaaggc gctcgttcgc cgccttggca
     3181 agaccttcac agtatctgag gattttccgg ccttcatcgt caaccgcatt ttgctgccga
     3241 tgatcaatga agcgatctat acgctttatg agggcgtggg ctccgtcgaa tcgatcgaca
     3301 cggcgatgcg gcttggcgca aatcatccga tggggccctt gcagctcgcc gattttatcg
     3361 ggctcgatac ggtgctgtcg gttatgcagc tccttcatga tggcatggcc gacccaaaat
     3421 accggccctg ccccttgctc gtgaaatatg tcgaagccgg ttggctcgga cgcaaaaccc
     3481 agcgcggttt ttacgattac cgcagcggta cgccaattcc gacgcgctag tttctcgcca
     3541 cgcgaactgt tgctttaagg tctcttcaca cgcctgtcat caaggccatg aaagactaat
     3601 cctggagggg ctagcgttcg attcgcagcc gttataagag ttccgagctg aaatagaagg
     3661 aggtcgcggc gctgccgctc gctggcagct cgctaggcga ggaaggtgat gtgacgattc
     3721 attcccaacc aagggtctcc cccgaggggt gcccggcgct tgtccttaac gccgacttcc
     3781 gtccattgag ctattatccg ctctcccttt ggtcatggca gaattcgatt aaggcagtct
     3841 ttctcgacag ggtgaatatc gtatcgagct acgataagac ggtccgaagt ccgagctttg
     3901 agatgcgtct tccctcagta atttccctta aaagctacgt aaaagcctcc cgatatcccg
     3961 cctttacccg tttcaatgtc tttctgcgcg accgttttag ctgccaatat tgtggctctc
     4021 gcgaggatct gacatttgac cacctcactc cccgctcaaa agggggcacg acgacctgga
     4081 aaaatgtcgt cgccgcctgt tccagctgca atctccgcaa aggtgatcag ctcccggccg
     4141 aggcccgcat gtggccgtcg cgcatgcctt atcagccgag tgtgaacgat ctccaccaaa
     4201 atgggcgccg ctttccaccc aattatttgc acgaaagctg gctcgattat ctctattggg
     4261 attcggtgct cgaaccctga gcgccctctt acccacgccg agaatcagct cagctcggcg
     4321 gtcaatctgc gcataaaagc aatgcagctt tcgacttgcg caatttcaat atattcgtcc
     4381 ggctggtgcg cttggtcaat tgagcctggt ccgcagacca ccgccggtag atcagccaat
     4441 tggaactgcc ctgcctcgga ggcgtaaggc acgcttattg tgcgattcga ccggccagct
     4501 tttagtgcca gactctcggc aattgacccg ctgactggct tgaggcctgg cacctcgacc
     4561 agcgtctcgg tttcaattga ggcatgcttg ggatagcgcg tgagttttgg aaccacggaa
     4621 cgtgcggcgt aattctcgag gtgccgaagc gctaggtttt gcgcgacatc gggaagagcg
     4681 cgaaactccc agtggaatgt gcagagcttc gccatgatat tgcgtgcggt tccgccttgg
     4741 atcatgccga catgaatcgt cgaggcggga gggtcaaaac gccgggaagg atcgccttca
     4801 gccatgagtt tcgcaccaaa gcggtagagt tcggtcacta gatcgcaagc ggcctcgatg
     4861 gcgctcgcgc caagggcggg ctttgaggaa tgcgcctcat ggccgtggac cacagttgcg
     4921 tatcccccca cgcccttatg tgcatcggcc acctgcatca aagtgggttc gcccacgagc
     4981 acagcacccg ggcatggcag accggtccca aactgcgcga tcgtatcgag cggaccacgg
     5041 caagtaattt cttcgtcgta gctcagaaga atgtggatcg ggcgcgtcag gcgcgtgttt
     5101 ttgaattcgg gaatcatcgc aaggcagacc gcgtcaaaac ctttcatatc gcaggttccg
     5161 cgtccgtaaa ggcggccatt ctcgcgacgc agtttgaaag gatcgctggt ccatgcttgc
     5221 cctgccacgg ggacgacatc ggtgtggccg gacagaacga tgccaccatc acagttttca
     5281 ccgatggtcg caaaaagcgc ggccttgtcg cccgccgcat tcggaatttt cacataggaa
     5341 acaccgaggg ctttgaagta atcctcgacg aaggaaacaa gagcaaggtt ggatttcgag
     5401 ctctcggtgt cgaagttgat aagacgctcg agaatgctga gcgttgcgtc aagcctttcg
     5461 tccatcgcat ggctcggcct gggcatcggc gggcgaaatc aataatctcg cacggacgcc
     5521 atgttcaacg aattcggacg gcagctcagt tgagaacacg ccgggcgtaa ggcgaatcga
     5581 gcgagaaaac gggtatctca gcttcgaatg actcaccctt ctcgtttacc atcttgtagg
     5641 tccccgtcat gataccggat ggcgttgaca tatttgtgcc cgatgtgtag cggaacgttt
     5701 caccgggctt caaaatcggc tgctcgccaa cgacccccgc gcccttaacc tcctcaagcc
     5761 tgccattgcc gtcggtgatc ttccaatgcc gggcggtcag ctgaactgtt acttcaccac
     5821 ggttggcgat ttcgacggtg taggcccacc aatacgacgc ctcatcggca tcggaccgct
     5881 cgggcacgaa ttccggaaga accgtgatct gaatgtcgtg agtgacgatg gtgtacatgg
     5941 atg
//
LOCUS       DATMPU010000034         5734 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_3442244,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000034 DATMPU010000000
VERSION     DATMPU010000034.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 5734)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5734)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5734
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3442244"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..351)
                     /locus_tag="VNY06_00755"
     CDS             complement(<1..351)
                     /locus_tag="VNY06_00755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX13404.1"
                     /translation="MNGDAAPAPALWRRDRFADLPSATGSTREENASREVRGLMLRRY
                     GHAHLDKAIDAFQSVHGLRLDWENSHSARPARKTSLFGKPALEAGRDEKARSVVALSR
                     VPRLAGFAGSPPAAD"
     gene            407..1537
                     /locus_tag="VNY06_00760"
     CDS             407..1537
                     /locus_tag="VNY06_00760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018407033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III PLP-dependent enzyme"
                     /protein_id="HWX13405.1"
                     /translation="MTERILQFLRSRREDGPRLVLDLDVVRENYAAFAKALPDTRVFY
                     AVKANPDPQVLKLLAELGSCFDTASVVEIEQALASGASADRISYGNTIKKERDIQRAY
                     ELGVRLFAVDCEAEVEKLARAAPGAKVFCRILCDSTGAEWPLSRKFGCAAELASRVLE
                     HAHRLGLTAMGLSFHVGSQQRNPRMWDGALKEAAAIFRDLAERGINLQMVNLGGGFPT
                     KYVKNIPAVKVYAQAILHSLRRHFGNHIPETIIEPGRGMVGNAGVIEAEVVLIARKSG
                     NDKVKWVYLDIGKFNGLAETMDEMIRYPIRTTFDGGATEPCVIAGPTCDSVDVLYERE
                     PYLLPVSLEIGSKVLIEGTGAYTTTYSSIGFNGFPPLKSYVI"
     gene            1685..2212
                     /locus_tag="VNY06_00765"
     CDS             1685..2212
                     /locus_tag="VNY06_00765"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014891197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HWX13406.1"
                     /translation="MALVSNFEARAPALWAGDTGTRNVTIGLESPADFWARERLLDTA
                     FGVARFEKTVERLREGRLPAEGLAFAAKDARGLIGTLRLWHIRAGGTPALLLGPLAVA
                     KACRSQGIGRGLIEEALWKATDAGHAAVLLVGDASYYEPFGFSRRHTLGLSLPGPVDA
                     QRFLGLELTPGAPKG"
     gene            2517..3947
                     /locus_tag="VNY06_00770"
     CDS             2517..3947
                     /locus_tag="VNY06_00770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012606114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HWX13407.1"
                     /translation="MPQWPVHGTITGPIVMIGFGSIGRGTLPLIERHFKFDKARLTVI
                     DPEDKDRHLLDERGITFVKKAVARDDFVDLLKPLLMLGGGQPFVVNLSIEVSSAAIMR
                     LCHELGALYIDTVAEPWAGFYTDPRLTASERSNYALREGVLDLRRALGEGPTAISCCG
                     ANPGMVSWFVKQALLNVAADCGIDAGRLSSREGWARLAQRVGVKGIHIAECDTQRAAA
                     PKPRGVFVNTWSVEGFVSEGLQPAELGWGTHEKHMPVEGNRHSFGPDCAIYLMRPGAG
                     TRVRSWTPTAKAQHGFLITHNEAISIADFLTLRENGEVGYRPTCHYAYRPADDAVLSL
                     DEMAGAQWRQQKKSHILDENEIVDGIDELGVLLYGHAKNAYWYGSQLSIEETRRLAPC
                     QNATGLQVTSAVLAGMVWALENPRAGIVEADELDFRRCLDVQRPYLGPVIGKYTDWTP
                     LEGRAVLFPEDVDKEDPWQFKNVLVR"
     gene            4170..5360
                     /locus_tag="VNY06_00775"
     CDS             4170..5360
                     /locus_tag="VNY06_00775"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HWX13408.1"
                     /translation="MDDKPLRQHTAGNGESAYHSRLPLVSIIIVNYNYGRYLREGFDS
                     VAAQTYPNIECIIVDNASTDESADVLREIERHFPGTKILRRKDNGGQSLAAQEGFKAS
                     SGEYVVFVDADDYLLDSFVETHVFVHLSLRIPVGFSSSDMFQAVGSRVVVGTVAYMSD
                     FIRSGRGSRPYLLRRIDEGAPQVWNLPGPGPSIETQVHLVEPSDSGDWMWAPTSGNCF
                     RRDALQLVMDNENLASLRSCTDSYLLRSVNVITGSVVIDRPLGVYRLHGVNVFSKHPH
                     LNGVVSYDRGGPSDHNQTGRKLIIDHLIANAEIFVEKMLSPLSYIGALKALDDSWPQL
                     QSSVPGCRSYLAGRVVNEAPALASALGYFYFVILLKRLRAAPHVIVKAWAKAWVNRKE
                     TKRD"
     gene            complement(5391..>5734)
                     /locus_tag="VNY06_00780"
     CDS             complement(5391..>5734)
                     /locus_tag="VNY06_00780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012383041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family NAD-dependent epimerase/dehydratase"
                     /protein_id="HWX13409.1"
                     /translation="DLIDGLDRLMNTPNEITGPINIGNPREFSIRELAEQILAITGSK
                     SKLIKKPLPDDDPKQRQPDITLAETVLGWRPTIELTEGLHATIAYFDTLLREQGKSAV
                     GSVKLSRAASR"
BASE COUNT         1131 a         1618 c         1725 g         1260 t
ORIGIN      
        1 atcagcagcc ggaggcgaac cggcgaaccc cgccaaacgg gggacgcgac ttaaagcaac
       61 gacggaccgg gctttttcgt ctctgccagc ctccaaagct ggcttaccga agaggctagt
      121 ctttcttgcc gggcgtgcgg agtgggaatt ttcccaatcc aagcgaaggc catgaacact
      181 ttggaaggcg tcaatcgcct tgtcaaggtg cgcatgtccg taacggcgca gcatcagacc
      241 gcgcacctcc cgcgaggcat tttcttcgcg agtggagccg gtcgcggatg gcaggtcggc
      301 gaagcggtcg cgtctccaaa gcgcgggcgc cggtgcggca tcgccattca cccgaagcct
      361 attctctctg gcgcgctttg tccgcgccct tcgaagggat gccgcgatga ccgaacgaat
      421 tttgcagttc ctccgcagtc gccgcgagga tggaccgcgc cttgtgctcg acctcgacgt
      481 cgtgcgcgaa aattatgcgg cctttgccaa ggcgctgccg gacacgcgcg tattttacgc
      541 cgtaaaagcc aatcccgacc ctcaggttct aaagctgctc gccgaactcg gatcctgctt
      601 cgatactgcc tcggtcgttg agatcgagca ggcgctagct tcgggcgcga gcgcggaccg
      661 gatcagctat ggtaatacga tcaagaagga gcgcgacatt cagcgcgcct atgagcttgg
      721 cgtgcgccta ttcgcagtcg attgcgaggc cgaggttgag aagttggcgc gagcagcgcc
      781 gggagcgaaa gtcttttgcc gtattttgtg cgacagcacg ggcgccgaat ggccgctctc
      841 gcgcaaattc ggctgcgctg cggaacttgc ctctcgcgtc ctcgaacatg cgcaccggct
      901 cggccttaca gccatgggcc tttccttcca tgtcggctcg cagcagcgaa atccgcgcat
      961 gtgggatggc gctttgaaag aggcagcggc gattttccgc gatttggccg agcgcggcat
     1021 caacttgcag atggtcaacc tcggcggtgg cttcccaacc aaatatgtga agaacattcc
     1081 ggcggtgaaa gtctatgcgc aggcgatcct ccattcgctg cggcggcatt tcggcaatca
     1141 tatccctgaa acgattatcg agccggggcg aggcatggtc ggcaatgccg gcgtgatcga
     1201 ggcggaagtc gtgctgatcg cgcggaaatc cggcaacgac aaagtgaaat gggtctatct
     1261 cgatattggc aagtttaacg gccttgccga gacgatggac gagatgatcc gctatccgat
     1321 cagaaccacg ttcgatggcg gcgccaccga gccttgcgtg atcgcgggcc cgacctgcga
     1381 ttccgtcgat gtgctctacg agagggagcc ttatttgctg cctgtcagtc tcgaaatcgg
     1441 ctcgaaagtg ctgatcgagg ggaccggagc ttatacgacg acctattcct cgatcgggtt
     1501 caacggtttt ccgccgctca aatcctatgt gatctgagtg tagcggctcg atcttcgcgc
     1561 gggggaaaac gcccccgcgt tcacccctaa acctaaatcc ggacggccag cgcaggctcg
     1621 atagctgagc tcgcggtgcg aggatcgtct cgtgcaaacc cggtataggc cggcggaggc
     1681 tcagatggcc ttggtctcga atttcgaagc ccgcgctcct gctttatggg ctggagatac
     1741 tggcacacgg aatgtcacca ttggcttgga atcgccagcc gatttctggg ctcgcgagcg
     1801 cttgctcgat actgcctttg gcgttgcgcg gtttgagaag accgtggagc gcttgcgcga
     1861 gggccgtctg cctgcggagg gcctcgcgtt cgcagcaaaa gacgcaaggg gcctcattgg
     1921 cacattgcgc ttgtggcata tccgggcggg cggtacgccg gccctcctgc ttggcccgct
     1981 cgcggtagcc aaggcctgcc ggtcgcaagg cattggccgc ggcctcatcg aggaagcgtt
     2041 atggaaggcg acggacgcag ggcacgccgc ggttctgctc gtcggcgatg catcttatta
     2101 tgaacccttt ggcttctccc gccggcatac gcttgggctt tctcttcccg ggcctgtcga
     2161 cgcccagcgt tttctggggc tcgagctcac gcccggtgca cccaaagggt aagaggaatt
     2221 gtcacggcag caggtgtgcg cgaatttccg gctcaccgta gcgaaattgc ggttcggcgc
     2281 gcggcctaaa gccaagtcga gccaagtcga aaccggtttt gcgtccggac gcgcgacgag
     2341 acaaagaatg ttgagcaaga tgacgtttcg aagaacaggc gcatttctct aagcggcctg
     2401 cttccgaggt gccccaaaat gccttcggag ttacaggagc cttggcttga agcttgaggc
     2461 tcattcctgt accgcagagc ttggctcagt tttccctgcc agattggaga aaataaatgc
     2521 cgcaatggcc cgtgcatggc acgattactg gtccgatcgt gatgatcggg tttggctcta
     2581 tcggccgcgg cacgctgccg ctcattgaac gccattttaa attcgacaaa gcacgactta
     2641 cggtcatcga tcccgaggac aaggatcgcc acctcctcga tgagcggggc ataacattcg
     2701 tcaaaaaggc cgtggcgcgc gatgatttcg tcgatctttt aaagccgctt cttatgttgg
     2761 gcggcgggca gccctttgtc gtaaacctct cgattgaagt gtcctcggct gcgatcatgc
     2821 ggctttgtca tgaacttggc gcgctatata ttgatacggt tgccgagcct tgggctggct
     2881 tttataccga tccgcggttg acggcatccg agcgttccaa ttacgccttg cgcgaaggcg
     2941 ttcttgatct gcgccgtgca ctcggagaag gtccgacggc gatctcctgc tgcggcgcca
     3001 atccgggaat ggtatcctgg ttcgtcaagc aagcgctgtt gaatgtggca gccgattgcg
     3061 gcatcgacgc cggaaggctg tcctcgcgtg aagggtgggc gcgattggcg caacgagtcg
     3121 gtgtaaaagg catccatatt gccgaatgcg atacccagcg cgcggcagcg ccgaaaccgc
     3181 gaggggtgtt tgtcaatact tggtctgtcg aaggctttgt atcggaaggc ttgcagccag
     3241 ctgaacttgg ctggggcacg catgaaaagc atatgccagt ggagggaaat cggcatagct
     3301 tcgggccaga ctgcgcgatc tatctgatgc gccccggcgc gggcacgcgg gtgcgctcct
     3361 ggacgccgac agccaaggca cagcatggct ttctcattac ccataatgaa gcgatctcaa
     3421 tcgcagattt tttgaccttg cgcgagaatg gggaggttgg ttaccggccg acctgtcact
     3481 atgcttaccg cccggccgac gatgccgtgc tgtccctcga cgagatggcc ggagcgcaat
     3541 ggcggcagca gaaaaagtcc cacatattgg atgagaacga gatcgttgac gggatcgacg
     3601 agctcggcgt ccttctctat gggcacgcga aaaacgccta ttggtacggt tcgcagctgt
     3661 ctatcgaaga gacgcgccgg ctcgcgcctt gtcaaaatgc gacgggtctt caggtcacct
     3721 cggccgtgct tgcgggaatg gtctgggcgc ttgaaaaccc gcgagccggt attgtcgaag
     3781 ccgacgaact cgacttccgc cgctgcctcg atgtgcaacg cccttatctt ggcccggtca
     3841 tcgggaagta caccgattgg actccgcttg aaggtcgcgc ggtgctcttt cccgaggacg
     3901 tcgataaaga ggatccgtgg cagttcaaaa atgtgctcgt gaggtgagga aagaaaaagg
     3961 cggccttgcg gctcaagcgc tgcaaggccg atctccatgc tgagcagccc gtgcgcgaaa
     4021 tctacgctgg cagtggcaga acggctgggc tccctctcca aagccatttg ctgtatgctg
     4081 tcgtcacgtc aaaccagacc cttctattcg ctgccgcttc cggatttaat acggaaactg
     4141 aagctgtcga gctgtgaaag agccgtgcga tggatgataa accgttgagg cagcacaccg
     4201 ccgggaacgg cgaaagtgcc tatcattcgc gtctcccctt ggtcagcatc atcatcgtca
     4261 actacaacta tgggcgctat ttgcgagagg gtttcgactc tgtcgctgca caaacctatc
     4321 caaacataga gtgcatcatc gttgacaacg cgtcgaccga cgagagcgcg gacgtcctcc
     4381 gagaaatcga aagacatttt ccaggcacca aaatactgcg gcggaaggat aatggcggcc
     4441 aaagcctagc cgcgcaggaa ggttttaagg caagctcggg cgaatatgtg gtctttgtcg
     4501 acgccgacga ctatctgctc gactccttcg tggagaccca tgtcttcgta catttgtcgc
     4561 ttcgcattcc cgtgggcttt tcctcatcgg acatgtttca agccgttggt tcgcgcgtgg
     4621 tggttggcac ggttgcctat atgagcgact tcatacgctc gggccgggga tcccggcctt
     4681 acctcctgcg gcggattgac gaaggtgccc cccaagtctg gaacttgcca ggccccggtc
     4741 cttccattga aacgcaagtc catctcgtcg aaccaagtga ctcgggcgac tggatgtggg
     4801 cgccaacttc aggaaattgc tttcgccgcg atgcacttca gctcgttatg gacaatgaga
     4861 atttggcgtc gctgcgcagt tgcaccgact cctatctcct tcgttctgtc aatgtcataa
     4921 cgggcagcgt tgtgatcgac cgcccgctag gcgtttatcg gttgcatggg gtgaacgtat
     4981 tttccaagca cccccatctc aacggtgtcg taagttatga ccgaggcgga ccttccgatc
     5041 ataaccaaac tggccgcaaa ttgataatcg atcatctcat agcgaatgca gagatatttg
     5101 tggagaaaat gctatccccg ttgagttaca taggtgcgct caaagccttg gacgattcct
     5161 ggccgcagct tcagtcctct gtgccgggct gccgcagtta tcttgcaggc agggtcgtga
     5221 acgaggctcc cgcgctcgct tccgcgcttg ggtattttta ttttgtcatc ttgttgaagc
     5281 ggctgagggc cgcgccccat gtcatcgtaa aggcctgggc aaaagcttgg gtcaaccgca
     5341 aagagacgaa gcgcgattaa accaatctta gcgcgctccg tccccgttgt tcagcgcgaa
     5401 gccgcgcggg aaagcttcac gcttccgacg gcgcttttcc cctgctcgcg caaaagcgtg
     5461 tcgaaatagg cgatcgtggc gtgcaatcct tcggtgagtt cgatcgtggg gcgccagccg
     5521 agtactgttt cggctagtgt aatgtcaggc tggcgctgtt ttggatcgtc atcgggcaga
     5581 ggctttttga tgagctttga cttcgatccg gtgatcgcga gtatttgttc cgcgagttct
     5641 cggatcgaaa attcgcgcgg gttaccgata ttgatcggcc cggtgatttc atttggcgta
     5701 ttcatgagtc ggtccagccc gtcgatcaag tcgg
//
LOCUS       DATMPU010000035         2380 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_3745488,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000035 DATMPU010000000
VERSION     DATMPU010000035.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 2380)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2380)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2380
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3745488"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..435)
                     /locus_tag="VNY06_00785"
     CDS             complement(<1..435)
                     /locus_tag="VNY06_00785"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014761233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase"
                     /protein_id="HWX13410.1"
                     /translation="MTASGALVLFSGGQDSTTCLALALDRHELVETIGFDYGQRHGVE
                     LDQRNIILHSIRRDFPAWRERLGADHLVRIEALRQISETAMTHDIEIKAGAKGLPNTF
                     VPGRNLLFLTLAAALGYRRGFKHIFAGMCETDYSGYPDCRDDT"
     gene            complement(573..1670)
                     /locus_tag="VNY06_00790"
     CDS             complement(573..1670)
                     /locus_tag="VNY06_00790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012496721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HWX13411.1"
                     /translation="MKDNHHLVDFEFTLALNNATGKYFLCRDVIEECGDLIDRVWYGL
                     CPTKQLPTRTMAKVLGRLAVLEISFRLKSRASTHLPLMHNPRPLVFSDPREVILYELK
                     PRDVVLCHDMGPVTHPDLYHPTVKPTYETAFDKIRKAKPLLVFVSECSLKTFVTLYGD
                     DYPSMKLIYPALRMGIHSGPEEPIAAAPQRFLLTVGSVGSRKNHLRSLEAFALSGLAE
                     LGYHYVICGGPEPGFDAVVESAGKIPAVVLPGYVSDNELRWLYAHASGFVLPSLLEGF
                     GLPAAEAIARDLVPLVSRGGALHEVAGDSAILVDPLNANEIADGMRRLALMAEPERQS
                     RLVELRRSIARFSRESAVGSWRATLQQALAT"
BASE COUNT          527 a          664 c          703 g          486 t
ORIGIN      
        1 ggtatcgtcc cggcaatcgg gataacccga ataatcggtt tcgcacatgc cagcaaaaat
       61 atgtttgaag ccgcggcgat agccgagagc tgctgcaagc gtcaggaaca agagattccg
      121 tcccggaaca aaagtgttgg gaagcccctt cgcaccggct ttaatctcta tgtcatgggt
      181 catcgcggtt tcggagattt gccgcaaggc ctcgatacgg acgaggtgat ccgcgccgag
      241 acgttcgcgc catgcaggaa aatcccgacg gattgagtgg aggataatgt tgcgttggtc
      301 gagttcgact ccgtgcctct ggccatagtc gaagccgatc gtctcgacga gctcatggcg
      361 atcgagcgcc agggcaagac aggtcgtcga atcctggccg ccggaaaaca acaccagcgc
      421 gccgctggcg gtcattgcga agcgctcgca aaacctgtca gctccggcag caagacgcgc
      481 aatcgcaatg gaaaggaact tgcgttcatc ggtgctcttt cacgccggcg gaagatgaac
      541 tccgcgcgcc tattttgcct tggcccttgc attcatgttg ccagcgcttg ttgcagcgtt
      601 gcccgccagg acccgaccgc tgattcccgc gaaaaacgcg cgatgctgcg gcggagttca
      661 acgaggcgcg actgccgttc cggctcggcc atcaaggcta gccgtcgcat cccgtccgcg
      721 atctcattcg cattcagcgg atcaacgagt atggcgctgt cgcccgcgac ttcatgcaaa
      781 gcaccccctc gactcaccaa cggaacgaga tcgcgggcaa tggcctcagc agcgggcaag
      841 ccaaatcctt caagcaggct tggcagcaca aacccgctcg cgtgggcata gagccagcgg
      901 agctcgttgt cactcacata acctggcaaa acgaccgcgg gtatcttgcc agcggattcg
      961 acgacggcat caaatcctgg ttccgggccg ccgcagatca catagtgata tcctagctcc
     1021 gcaagcccgg aaagggcaaa agcttcgagc gaccgaaggt ggtttttgcg tgatcccaca
     1081 ctcccaaccg tcaacaaaaa gcgctgcgga gcggccgcga taggctcctc tgggccggag
     1141 tgaataccca tccgcagtgc cggatagatc agcttcatcg aggggtaatc atcgccatag
     1201 agcgtaacga aggttttcag cgagcattcg ctgacgaaaa ccagcagggg tttggctttc
     1261 cgaattttgt caaaagctgt ttcataagtg ggtttgacag ttggatgata gagatcggga
     1321 tgtgttaccg gtcccatatc gtggcagagc acgacgtctc gaggttttag ctcataaagg
     1381 atcacctccc ttggatcgct aaacacgagc ggccgcgggt tgtgcataag cggcagatgc
     1441 gtggatgccc gtgacttgag cctaaagctg atttcgagaa cagcgagacg ccccaaaacc
     1501 ttcgccatgg tgcgagtggg aagctgcttc gttgggcaga gcccatacca aaccctatcg
     1561 atgaggtcgc cgcattcttc gatcacgtct cggcagagaa aatacttgcc cgtggcatta
     1621 ttcagagcca gggtgaactc aaaatcgaca agatggtgat tgtccttcat tggctgcctc
     1681 aaagcggtgc ggcgcggact gagttccggc gtagatcggt gccggtatcg tgcgggaccc
     1741 gcggtctgcc atcgcgcaca aggtcgatac gcgaacgaac gccaaaatgc gggcacaggc
     1801 agttcgaccg cggcaaacct tgaaatcaca gggcggatga taggataaat tgtcatcaat
     1861 aaagttgaag cgcgccgacg acttgctgcc tggcggaagg ctcggcgtcc gcatattcga
     1921 agcgccaaag attagcaggt aatttataga ttgcatgtgt acatcgcttc actggcgggg
     1981 tccaccatgc gtgaggcaaa aatgcggaca gtgccagacc agaatgtcgt ccggcggatt
     2041 gttctttcac gcgcccgagc ggctgctttc gatggcttgc tttccttgac agcgccgctg
     2101 tgccgagggt acccctcgtg gcggtcggtt gggtcccacc tcctcgtccc gcatgactga
     2161 aatcgatggg agaggatttt ggactcggcg cattgggcct gggccgcggc ttgtgtcagc
     2221 cggcacgcat gccgcgtgca gacagtccgg agagcgctgc gggattacgt ttgtgcagga
     2281 aatcgattcc gtaaaaattt gtcgcgccag tctttttgat cttgaatgag gcccgttggt
     2341 gacaaatgcg gatgggctga ccatcaacgg ccgttttctc
//
LOCUS       DATMPU010000036         2779 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_3745841,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000036 DATMPU010000000
VERSION     DATMPU010000036.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 2779)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2779)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2779
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3745841"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..271
                     /locus_tag="VNY06_00795"
     CDS             <1..271
                     /locus_tag="VNY06_00795"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012589405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GTP cyclohydrolase I"
                     /protein_id="HWX13412.1"
                     /translation="GLSKLARLVDTFARRLQTQEALTAQIIGAIDRHLRPRGCAVMLE
                     AQHMCMSMRGVRKHGSETITTQFTGIFRDDPAEQIRFLTMLRDSK"
     gene            351..743
                     /gene="hisI"
                     /locus_tag="VNY06_00800"
     CDS             351..743
                     /gene="hisI"
                     /locus_tag="VNY06_00800"
                     /EC_number="3.5.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018044035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="HWX13413.1"
                     /translation="MTSVPTQSELEEGEAFTPRFGANGLIVCVTRDAADGQVLMLAYM
                     NEEALRLTIETGVAHYWSRSRQTLWRKGDASGRIQKVTEMRTDCDQDALLLTVEPGGD
                     GGACHTGRRSCFYRKIALRSDPGKLVFD"
     gene            1092..2051
                     /locus_tag="VNY06_00805"
     CDS             1092..2051
                     /locus_tag="VNY06_00805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020175837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HWX13414.1"
                     /translation="MRRPKIGIALGAGSARGWSHIGVLLELLAHKINPEIVAGTSIGA
                     VVGGAFAAGKLEEMEAFARSLTKRRVMSMMDVSFSGVSFLGGERLRRELEKEFSGLNF
                     EDLEKPFGTVATEFGSGHEVWLTKGNVSDAIRASYTLPGIFEPVNINGRWLFDGALVN
                     PIPVTLCRALGAEIVLAVNLVSDPSYRGATIHDPRSIEPALDKFHFSPPETAKTLSAR
                     IFDGVSGNLRRVFGKREDGAPGIANAMMDAFNIAQARISRARLAGDPPDVTITARLGK
                     IGLFDFHRADEMIAAGCDAARKALPTILEDIVLALPEDVDAPR"
     gene            complement(2068..2499)
                     /locus_tag="VNY06_00810"
     CDS             complement(2068..2499)
                     /locus_tag="VNY06_00810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012454115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HWX13415.1"
                     /translation="MIVAHILAAKGREVVATQPHRTLAEAAALLTARKIGAVVVADAQ
                     GRILGVLSERDILHAVGNGGAAALEDAVSKHMTTEVLATHENETVDRTMEKMTHRRCR
                     HLPVVEDGKLAGIVSIGDVVKHRLEELEHEHQAMREYIATA"
BASE COUNT          600 a          832 c          767 g          580 t
ORIGIN      
        1 cgggctttcg aagctcgcgc ggcttgtcga tacgttcgcc cgacggttgc aaacccaaga
       61 ggcattgacc gcgcaaatta taggcgcgat cgaccggcat ttgcgccccc gcggctgcgc
      121 cgtcatgctc gaagcccagc acatgtgcat gtccatgcgc ggcgtccgca aacacggctc
      181 ggaaaccatc acgacgcagt tcaccggcat atttcgtgac gatcccgccg agcagatccg
      241 tttcttgacc atgctgcgcg acagcaaatg accgctgcct cgcgagcctt tgcatccttc
      301 cggtccggaa tttgcaccaa aagcggcgtg agccggcgcg gaattccggc atgacatccg
      361 tgcccacgca aagcgaattg gaagaaggag aggcgtttac gccgcgcttc ggcgccaatg
      421 ggctgatcgt ttgcgtgacg cgcgatgcgg ccgacgggca agtcctgatg cttgcttaca
      481 tgaatgagga ggcattgcgt ctgacaatcg agaccggcgt cgcgcattat tggtcgcgct
      541 cgcgccagac tctctggcgc aagggcgacg cttcaggccg aatccaaaag gtcacggaaa
      601 tgcggacaga ttgcgaccag gacgctttgc tattgactgt cgaacccggt ggcgacggcg
      661 gcgcatgcca taccggacgg cgttcctgct tttatcgtaa gattgcgctt cgcagcgatc
      721 ccggcaaact tgtattcgac tgaataaacc ccgatttaag attccaagtt cggctcgcaa
      781 agcaaaacgg gtgaggccaa tcgatattgg cgcaactgcg aaaaaacatc ggtgtttatt
      841 ttgtcaaatt cacgtgttcg agaggaacat tcagataacc ttcaggcatt ttcaatcatc
      901 aagcgagaca attgtgagca gtggaaagga tttctgatca aagccagcct cgcattggcc
      961 gctcggcaaa accgtgaccg tctaggcgag cgcgcttccc cctaggacaa attcaaatga
     1021 accacatacc tttcttatcc aagagcaccg ctaacagcgt catgggtcaa agagccgcca
     1081 actccgcgga aatgcgcagg ccaaaaattg gtatcgcgct cggcgccggc tcggcgcggg
     1141 gctggtcgca tattggcgtc ttgctcgaac tcctcgctca caagattaat cctgagatcg
     1201 tggctggcac atcaatcgga gcagtggtcg ggggcgcctt tgcagctggc aagcttgagg
     1261 agatggaggc gttcgcccgc agcctaacca aacggcgcgt catgagcatg atggacgttt
     1321 cgttttcagg ggttagtttc ctcggcggcg aaaggctccg ccgcgagctt gaaaaagaat
     1381 tctctggcct aaacttcgaa gatctagaaa aaccgtttgg aacggtcgca accgaattcg
     1441 gctcgggtca cgaagtttgg ctgaccaaag gcaacgtctc cgacgctatt cgggcttcct
     1501 acactttgcc tggaatcttc gaaccggtga acatcaacgg gcgctggctc tttgacggcg
     1561 cgctcgtcaa tccgatccca gttacgctct gccgggcgct tggcgcagag atcgtgctcg
     1621 cggtcaatct cgtcagcgac ccatcctatc gaggcgcgac catccacgat ccacggtcga
     1681 ttgagccagc gctcgacaaa ttccatttta gccccccgga aacggccaaa acgctgagcg
     1741 cacgaatctt cgatggcgtc agcggcaatt tgcgccgcgt gttcggcaag cgggaagatg
     1801 gcgcgcccgg gatagccaat gccatgatgg acgccttcaa tatcgcgcaa gccaggattt
     1861 cacgcgcgcg cctcgccggc gatccacctg atgtcaccat cactgcgcgg ctcggaaaaa
     1921 tcggcctctt tgattttcat cgtgccgacg aaatgatcgc agccggctgc gacgcggcgc
     1981 gcaaggcact gcccaccatc ctcgaagaca tcgtcctggc tctgcctgag gacgtcgacg
     2041 ctccgcggta ggtttggccg gccactctta tgcggtagca atatattcgc gcatcgcctg
     2101 gtgctcgtgc tccaattcct cgagccggtg tttcaccacg tcgccgatag acacaatccc
     2161 ggcgagcttc ccatcctcaa cgacgggcag atggcgacaa cggcggtggg tcattttctc
     2221 catcgtcctg tcgacggtct cgttttcatg cgtcgccaag acctctgttg tcatgtgttt
     2281 tgagacggca tcctcaagcg cggcagcgcc gccgtttcca accgcatgaa gaatatcgcg
     2341 ttccgatagc actccgagga tgcggccctg cgcgtcggcg acaaccactg cgccgatttt
     2401 ccgcgccgtc aaaagcgctg cggcttcagc caaagtccgg tgcggctggg tggcgacgac
     2461 ctcccttccc tttgccgcaa gaatgtgtgc gacaatcatt tgcaatccct tcttgattat
     2521 catcgtttat gggttccgcc ctgaaaaccc ggcgcctcgg cgccggtctc cagagaaaga
     2581 aaattgcctc tttagaatgc acctgttctt gaggacgcgc aagtatagtt tgaaagccct
     2641 tcactatcgt ccaagcagcg ttgcattgca gcaccacaat tctttggaag agggcagcga
     2701 ataccgcgcc gcaaaatccc ggcaaccgac cgcaaaattc aagcttcaat ttgcgatagc
     2761 ttgcacggca gaggtgcca
//
LOCUS       DATMPU010000037         3934 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_26895, whole
            genome shotgun sequence.
ACCESSION   DATMPU010000037 DATMPU010000000
VERSION     DATMPU010000037.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 3934)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3934)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3934
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_26895"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(120..1844)
                     /locus_tag="VNY06_00815"
     CDS             complement(120..1844)
                     /locus_tag="VNY06_00815"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWX13416.1"
                     /translation="MQQDISRGSISGLLVKPGLSSQEIVEILAILPSGLASHMPVFAL
                     GGKHAEHPSELILASADFLVLCGATDFDGLTHLVELSDSGRPDLLADFAAKFIIDATA
                     STERLQLRIASMTRTIAIFGGRVTAERAPPLFVGVVVDVVDRDEEAPALTESSGRTVN
                     LARGSDKTRLTAPRSRSSFYPQPGAKSSRESLEAELRRPKVAFRELSFILDTATDGVV
                     VLDWQGLILTLNRSGEALFGYDQHEVQGKPLTSLLAPESSPLALEYFEGLKSRVSSLF
                     NQGREVTVLAREGGTLPVFMTLAQIGAAGSGTPREERFCALLRDLTHWKNVEQELEAA
                     RKEAERANALKSAFLAKVSHEIRTPLNAILGFAEVMIEERFGPIGNPRYKDYIGDIHA
                     SGALVMSLVNDLLDLSKIEAGKMDFAFASIDVNRVVSECVSIMQPQASREQVDTKLSL
                     SPALPNILADERALRQIVLNLLSNAVKFNRPGGQVIVSTLLADSGSVVVRIRDTGLGM
                     REGDIGVAFEPFRRLDAGRPSRGTGLGLPLTKALVEANGASMTFKSKEQEGTLVEVVF
                     PSARILAE"
     gene            2044..2120
                     /locus_tag="VNY06_00820"
     tRNA            2044..2120
                     /locus_tag="VNY06_00820"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2078..2080,aa:His,seq:gtg)
     gene            complement(2181..3113)
                     /locus_tag="VNY06_00825"
     CDS             complement(2181..3113)
                     /locus_tag="VNY06_00825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX13417.1"
                     /translation="MSLSPDDAASQPNSAADRKLSENVPAETRWEGKSRQATRPGLIP
                     ITNAERGFWQDLPPRLEHLVSFGPQVALATCLFGFAWAAGSYFSGDQSSLFSRPKPPA
                     SPSIAQHERIERAELAREVHKMAADIRALQASMETLRDEQGQAAKTRSSLDGLTGRLD
                     AVRNEASAAIAAMAVKAGRVQREPEAKMTQVIDRLDRLERKLTTSAAMASAAPAAAGA
                     TQRTAQIAADPARLPQDGADGSRKSHLITNWVVRDVYGGIALLESTHGTIEVAPGEII
                     PGAGRVKSIERRGPGWIVITSQGLVDSARDVFLP"
     gene            3255..3359
                     /locus_tag="VNY06_00830"
                     /pseudo
     CDS             3255..3359
                     /locus_tag="VNY06_00830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007674967.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
BASE COUNT          832 a         1145 c         1110 g          847 t
ORIGIN      
        1 ggctctcctt gcgactgctt ttttggtccg tttctctggc ctttgtcgtc gcggtcataa
       61 tgtcctccct tagctatgga tcaagagaac gatcgtaacc tgcaacggtt ccttgtgttt
      121 cattccgcca gaatgcgtgc cgaagggaag acgacctcaa ccaaagtgcc ttcctgctcc
      181 ttgctcttga acgtcatcga agctccattg gcttcgacca aggccttggt gagcggcagg
      241 ccgagacccg tgccgcgcga cggccgtccg gcgtcgagcc tccggaaggg ctcgaaggca
      301 acgccgatat cgccttcccg catgccaagg ccggtatcgc gaatgcgaac gacaacgctg
      361 ccggaatcgg caagcagcgt ggacacgatg acttggccgc ccggccggtt gaacttcact
      421 gcgttcgaca gaagatttag gacgatctgg cgaagcgcgc gctcgtcggc aagaatgttt
      481 ggaagcgcgg gcgacaagga gagctttgtg tcaacctgct cgcggcttgc ctgcggctgc
      541 atgatcgaga cacattcgga gacgacacga ttgacatcga tcgatgcgaa agcgaagtcc
      601 atcttgcctg cttcgatttt ggaaagatca agcagatcgt tgacgagact catgacgagc
      661 gcgccagagg catgaatatc gccgatataa tccttgtagc gcggattgcc gatgggaccg
      721 aagcgctcct cgatcatgac ttcggcaaag ccgaggatag cattgagcgg cgtgcgtatt
      781 tcgtggctca ccttggcaag gaaagccgat ttcagcgcgt tggctctttc ggcttccttg
      841 cgtgccgctt caagctcctg ttcaacattt ttccaatggg tgaggtcccg caagagggcg
      901 caaaaccgct cttccctcgg cgtgcccgag cccgccgcac cgatttgcgc aagcgtcata
      961 aagaccggaa gcgtcccgcc ttcacgtgca agaacggtca cttcgcgtcc ttgattgaag
     1021 aggctggaga cgcgcgattt tagcccttca aaatattcaa gcgccagcgg cgagctttcc
     1081 ggcgcgagca aggatgtcaa gggtttgcct tgaacctcat gttgatcgta accgaacagt
     1141 gcctcgccgg aacggttcaa tgtcaggatc agcccttgcc aatcgagcac cacaacgccg
     1201 tctgttgccg tgtccaagat gaagctcagc tcgcgaaacg cgactttcgg gcgccgcaat
     1261 tccgcttcga gggattcacg gctgcttttg gcgcctggct gcgggtaaaa agaactgcgc
     1321 gaacgaggcg cggtcaggcg cgtcttgtcg ctgcctctag cgaggttcac cgtccgccca
     1381 gagctttcgg taagcgcggg ggcttcctca tcccggtcga ctacatcgac gacgacgcca
     1441 acaaacaagg gcggcgctct ttccgcggtg acacggccgc caaaaatagc gattgttcgc
     1501 gtcatgctcg caatgcgcaa ttgcaatctt tcagttgagg ccgtagcatc gatgatgaat
     1561 tttgccgcaa agtcagcgag cagatcgggg cgcccgctgt cgctgagctc aaccaagtgg
     1621 gtaaggccat caaaatccgt ggccccacat aaaacaagaa aatctgcgct ggctagtata
     1681 agttcggagg gatgctccgc atgcttgccg cccagtgcga aaacaggcat atgcgacgca
     1741 agtccggatg gcagaattgc caaaatttcg acgatctctt gcgagctcag ccccggctta
     1801 actaagagcc cagagatgct gcctcgagat atatcctgct gcatggccga ccttcatgaa
     1861 agttgagatt gtgcagcgta gttcggccca aagccgcacc ctattgggcg aaacgatctc
     1921 ttcgttgtga agcgaaacgg ctgagataag acagtatttc gatctggttt tctctcgtgg
     1981 caggccctta ttcaaggtct tgcaatcgcg gatcgatcct tttacgttcg cgcgctccgg
     2041 tacgccgcca tagctcagtt ggttagagcg ctagattgtg gatctagagg tcccccgttc
     2101 gagccggggt ggcggtacca tgtaacgaca tctctccttt gatcgttaaa gaaccgaacg
     2161 caagcaccct tcagcgccta tcagggcaag aaaacgtcgc gggctgaatc aacaagcccc
     2221 tggcttgtga tcacgatcca gccggggccg cgcctttcaa tcgactttac ccttcccgca
     2281 ccaggaatga tttctccggg cgcgacctcg attgtaccat gggtgctctc gaggagggcg
     2341 ataccgccat aaacatcgcg gacgacccag tttgtgatga gatgcgactt tcttgatcca
     2401 tcggcgccgt cttgcggcag ccttgccgga tcagcggcaa tctgcgccgt tctctgggtg
     2461 gcacctgctg cggctggcgc cgcgctggcc attgcagctg aagtggtaag cttgcgctcc
     2521 agacggtcga gccggtcgat cacttgggtc attttcgctt ccggttcgcg ctggacccgt
     2581 cccgccttga ccgccatcgc ggcaattgcg gcgctggcct cgttcctaac tgcgtcaagc
     2641 cggcctgtca gtccatcaag gctcgacctc gtcttcgcgg cttggccctg ctcatcacgt
     2701 aaagtctcca tgctggcttg gagcgcccgg atatccgcgg ccattttatg cacttcgcga
     2761 gccaactcgg cgcgctctat tctttcgtgc tgcgctatgc tgggtgaagc cggcggcttt
     2821 ggacgggaga aaagagacga ttgatcgccg gaaaaatatg atcctgccgc ccaagcaaag
     2881 ccaaacaaac aggtggccaa agccacctgc ggaccgaagc tcaccaaatg ctccaaccgc
     2941 ggcggcaagt cttgccaaaa acctctttcg gcgtttgtaa tcgggatcag gccagggcgc
     3001 gttgcctgcc gggatttacc ctcccatctg gtttccgctg ggacattttc gctgagcttt
     3061 cggtctgcag cagaatttgg ctgagaagcg gcatcgtctg gcgaaaggct catacgcatg
     3121 ctccaaggat agttaaggca tggtaatcat tccctgcctc tattcatctt aaagttgcgt
     3181 taacggattg gcattttgcg caaacaaaca accgcgcgcg acgaactcag atggccgcca
     3241 atcgttatac ttaaacttat ggagctgcag ccagtgatct tggaattgca aactaccatg
     3301 aacgcggtcg atggcgcaac aatcgagccg agaattcgta tcagccaacc cgacgaaggg
     3361 tggtaccgtg acagagcccg ttcgcgagtt tgccggtgcc gttgggaacc ttcatgcgga
     3421 cgcgcccaga ggagcacaca tgagtaacga acgaggcggc tggtgcagtg cgctcgaagc
     3481 ctccgttccc cagtcgcgca ggaagaaatt cgacacaaat ttccttgccg cgtactgagc
     3541 tgcatgcggg agctgcttct gagcagtttc gaagtgaagt gtctgaagcg gccgagatgg
     3601 ggccttgaca aggcgccgac gcctccacgt cccgatttgc gcaagattga acccaacttc
     3661 gcaagatccc ccaagcgcga aacttcactg caagctacga aaccaggaca ccactagcat
     3721 ggaggcgatg atgcttcgga gcggcgattt tctcttaaag gttggcacgg gaagagcggt
     3781 tttgtctggt ctccttttag ccgtcgctca agccagcgcg gttcaagctc agcctttgga
     3841 ttccgcgaac gcgccacccg cgccgcgcca tcggccggcg agtttgctga atgaaaataa
     3901 tttgacttca accgggcaga cggtgccccg cccc
//
LOCUS       DATMPU010000038         2095 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_3126145,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000038 DATMPU010000000
VERSION     DATMPU010000038.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 2095)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2095)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2095
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3126145"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..276
                     /locus_tag="VNY06_00835"
     CDS             <1..276
                     /locus_tag="VNY06_00835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086557984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HWX13418.1"
                     /translation="LSATMSASSASCAPRPAGTRSSTPEPIKDWSLTTLKEKLIKIGA
                     KIVSHGRYVTFQMAEVAIPKTLFADILQLIAELRPPPAALNQGRGTS"
     gene            complement(497..970)
                     /locus_tag="VNY06_00840"
     CDS             complement(497..970)
                     /locus_tag="VNY06_00840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX13419.1"
                     /translation="MAENDSRDDVRTLYREAARTRTDDDRAIVEKIDRLTQSIGRAEQ
                     EIAQLRGQIREVAARRAEIDQARGEFRQRGYDYPGTTFGNETTINDVLGGILEGAMKG
                     IVLGQVLDQGYRRPPASNWGGGGVLPGPMYPPSGHYPVPPMGPSSGDGFRTGGSF"
     gene            complement(1115..1327)
                     /locus_tag="VNY06_00845"
     CDS             complement(1115..1327)
                     /locus_tag="VNY06_00845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX13420.1"
                     /translation="MAVRAAAGHAKIAALINYREARANYAMLNQIPERLRDHANRVGA
                     EFSAERQKLAAFEQDRLIAAGGGPLQ"
     gene            1462..1974
                     /locus_tag="VNY06_00850"
     CDS             1462..1974
                     /locus_tag="VNY06_00850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007674967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HWX13421.1"
                     /translation="MTKISYDGYRFPPEIIQQAIWLYLRFTLSFRDVEDPEGEVLDVL
                     IQSWRNKRAALKLMRKLLRKYGYVPDKLVTDDLRSYAAAVHDLGLSNRHERGRWRNNR
                     AENSHQPTRRRERKMQGFKSPGSAQRFLSTHATTYNTFNVQRHLTSARTHRTFRASAM
                     NIWRAAVAAA"
BASE COUNT          444 a          642 c          592 g          417 t
ORIGIN      
        1 ttgagtgcga ccatgagcgc atcgagtgca tcgtgcgccc cgcggccggc cggaacccgt
       61 agctcgacgc ccgagccgat caaagactgg tcgctgacaa ccctgaaaga gaagctcatc
      121 aagatcggcg caaagatcgt cagccacggg cgctatgtca ccttccagat ggcggaggtc
      181 gccattccca aaactttgtt cgccgacatt ctgcagctga tcgcggaact gcggccgcca
      241 ccggcggccc ttaaccaagg gagaggtacg tcttgatgcc aacaaaatcc gcatttccgg
      301 cgctcggagc gccgatgtca ctagcttggg tgcacgagag ccccgtgtcg agggtttggc
      361 attgccaaaa tccgtcgaaa gtcgcgatct cagcatcaac aaggccggca tccggtggat
      421 accggttaag gcagtttcat tggatcgcga gttggagatc tctatgatcg atattttcag
      481 aaaattctcc aaaatcctaa aacgaaccgc cggtacgaaa cccgtctccg ctggaaggac
      541 ccatcggggg gacaggataa tgacctgagg gtgggtacat cgggccaggc aacacgcccc
      601 cgccgcccca gtttgacgcg ggcgggcgcc gatagccctg gtcgaggacc tggccaagga
      661 caatgccctt catggcgcct tccaaaatgc cgccaagcac atcattgatc gttgtctcat
      721 tgccaaaggt ggtacccgga taatcatagc cacgctgccg gaattcccct cgcgcctggt
      781 cgatttcggc gcgtctggca gcaacttcgc ggatttgccc acgcagctgc gcaatttcct
      841 gttcggcgcg accaattgat tgcgtgagcc ggtcgatctt ttccacgatc gcgcgatcat
      901 cgtccgtcct ggtacgcgcg gcctcgcggt agagcgtgcg cacatcgtcg cgggaatcat
      961 tctcagccat gagctcgatc gccttggtaa aggcgccatt gttatcccgc ccggcgatgg
     1021 catgatactg ttggtcaagt acctccagcc tttggcgcgc ctgcgcaacc tgcgcctcac
     1081 tggaatcgag cgctgctttg gcttctgctg ccttctattg caaaggacca ccgcccgccg
     1141 caatgagccg gtcctgctcg aaggccgcga gcttttgccg ctcggcgctg aattccgcac
     1201 caaccctatt ggcatggtcg cgaagccttt ccggaatctg attcagcatc gcgtaatttg
     1261 cgcgcgcttc acgatagttg ataagtgcgg cgatctttgc gtggcccgcg gcagcgcgga
     1321 ccgccaatgg gcgcgcatct ggccgacgcg agaaaagagc ccccaaaatg ctaggcgtgg
     1381 cgaaacttgt gagcggctac cgcggcattg tcacggcggt tcgggcttgc gggcttgctg
     1441 gcaattccgg tagccaaggg gatgacgaaa atcagctacg acgggtaccg atttccacct
     1501 gagatcatcc agcaggcgat ctggctctat ctccggttca cgctgagctt ccgcgacgtc
     1561 gaagatcccg agggtgaggt cctcgacgtg ctgatccaga gctggcggaa caagcgcgcg
     1621 gcgctgaaac tgatgcgcaa gctgctgaga aaatatggct atgtcccaga taaactggtc
     1681 acggacgacc tgagatctta tgctgccgcc gtccatgatc tcgggctctc gaaccggcat
     1741 gaacgaggcc gatggcgcaa caaccgagcc gagaattcgc atcaaccgac ccgccgacga
     1801 gaacgcaaga tgcaaggttt caagagtccg ggttcagccc agagatttct ctcaacccac
     1861 gcaaccacct acaatacttt caatgttcaa cgccatctca cctcagcaag aacgcaccga
     1921 acctttcgcg cgtcggcgat gaacatatgg cgcgcggcag ttgcggccgc ttgacaatat
     1981 gcgagccgca cgtttctcgc gttcttctta cgacaacgtg acagcacctt gtgtgcagct
     2041 ccaagcgtta acttccctga aaccataacg gtctcctatg gcgcagggag tgcgc
//
LOCUS       DATMPU010000039         4096 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_1577778,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000039 DATMPU010000000
VERSION     DATMPU010000039.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 4096)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4096)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4096
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1577778"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..244
                     /locus_tag="VNY06_00855"
     CDS             <1..244
                     /locus_tag="VNY06_00855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX13422.1"
                     /translation="DLAAEFSLTEIDLPLDYMNGIFCSTALAQLGGKSDIDPQQLVNN
                     LTDDYPRARRNARDALISLGPSAVPRVLVTVFTAIR"
     gene            complement(241..2046)
                     /gene="lepA"
                     /locus_tag="VNY06_00860"
     CDS             complement(241..2046)
                     /gene="lepA"
                     /locus_tag="VNY06_00860"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019402463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HWX13423.1"
                     /translation="MTSHVLKNLRNFSIVAHIDHGKSTLADRLIQSTGTLAEREMVDQ
                     VLDSMEIERERGITIKAQTVRLEYKAQDGEIYILNLMDTPGHVDFAYEVSRSLAACEG
                     SLLVVDASQGVEAQTLANVYHATDAGHEIVPVLNKIDLPAAEPERIRQQIEDVIGLDA
                     SQAVLISAKTGEGIGKVLEAIVGRLPPPRGDETAPLKALLVDSWYDAYLGVVVLVRVI
                     DGMLKKHQRIKMMGTGAQYEIEKVGVFRPKMQDAAELGPGEIGFITAQIKEVADTRVG
                     DTITDVRHPCVQPLAGFKAAQPVVFCGLFPNDAADFGDLRSAMGRLRLNDASFTYEME
                     SSAALGFGFRCGFLGLLHLEIIRERLEREFNLDLIATAPSVIYRIVLRGGGNLELHNP
                     ADMPDPTKIEEIEEPWIRATILTPDDYLGAVLKLCQERRGVQADLSYAGKRAIVRYDL
                     PLNEVVFDFYDRLKSISKGYASFDYAITGYRPGDLVKMSILVNAEPVDALSMLVHRDR
                     AEARGRAMVEKLKELIPQHLFQIPVQAAIGGKIIARETVRALRKDVTAKCYGGDVTRK
                     RKLLEKQKAGKKKMRQFGKVEIPQEAFIAALKMDS"
     gene            2218..2769
                     /locus_tag="VNY06_00865"
     CDS             2218..2769
                     /locus_tag="VNY06_00865"
                     /inference="COORDINATES: protein motif:HMM:NF017416.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HWX13424.1"
                     /translation="MRGAVLETSPIWVISDSIVTAGRETIGRQPGFFDVDDRLKRLSD
                     LGDQLEAFRAVVDFEIFRPELKAALAYSDGVRGGGRPFDPGMMLKILAIQTTNTRSDE
                     RTEFLINDRLSFMRFLRLKLSDRVPDARTIWVFREKLTKAGAVGPLFERFDVTLRQSG
                     YIAMSGQIVDASLIAAPRRRVLS"
     gene            2800..3177
                     /locus_tag="VNY06_00870"
     CDS             2800..3177
                     /locus_tag="VNY06_00870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX13425.1"
                     /translation="MLKPGENSCSISWDRISENEFAAVVAETLAALFPQDPPEFLIRI
                     PYGYFDVKSIHDELSRAGFTSNEAISLASVTRASSPHDAAIAYCHGTPLRNEIEARDL
                     SRVAEATQAVAEALASRFGNGPR"
     gene            complement(3386..>4096)
                     /locus_tag="VNY06_00875"
     CDS             complement(3386..>4096)
                     /locus_tag="VNY06_00875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012332995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009037 - tyrosine-based site-specific
                     recombinase activity [Evidence IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA];
                     GO_process: GO:0007049 - cell cycle [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HWX13426.1"
                     /translation="GAGNRTLLRQLAGLRSFARYLERNGKGKSVAFASLKAPRVKRSL
                     PKPLTIAAARQVILADASAGENQCQWVVARDSAVLCLLYGAGLRISEGLCVKRSEAPV
                     AGRDFLTVTGKGQKTRTVPIIAPVRQAVEAYLALCPYQLVPDGPLFVGVKGGPLSSRI
                     IQLAVARLRGALGLPDSATPHALRHSFATHLLERGGDLRSIQELLGHASLSTTQLYTA
                     VDSTRLLESYRAAHPRGR"
BASE COUNT          885 a         1198 c         1108 g          905 t
ORIGIN      
        1 tgatctggcc gcggaatttt ctttaaccga aattgatcta ccgttggatt acatgaacgg
       61 catattctgt tctactgcct tggcgcaatt aggcgggaag agcgatatag atccacaaca
      121 gttagtcaat aacctaacag atgactatcc aagagcgcga cgcaacgcgc gcgatgctct
      181 aatctcgctt gggccttccg cagtgccccg tgtgctagta acggttttta ccgcaatccg
      241 ttaactatcc atctttagcg cggcgataaa agcctcctgt ggaatctcga ctttcccgaa
      301 ttgccgcatc ttcttctttc ctgccttctg cttctccaaa agcttccgct tgcgagtcac
      361 atcgccgcca tagcatttcg cagtgacgtc cttccgaagc gcccgcaccg tttcgcgtgc
      421 tatgattttt ccgccaatcg ccgcctgaac ggggatctgg aataaatgtt gcggaataag
      481 ttccttgagc ttctcgacca tggcacggcc gcgcgcctcg gcgcggtcgc gatggacgag
      541 catggagagg gcatccacgg gttcggcgtt gaccagaatt gacattttaa cgagatcgcc
      601 tgggcggtag ccagtgattg cataatcaaa actcgcataa cctttcgaaa ttgactttag
      661 ccggtcatag aaatcgaata cgacctcatt gagcggcaaa tcatacctca caatcgcgcg
      721 tttgcctgca tagctcaaat cggcctgcac gccgcgccgc tcctgacaaa gttttaacac
      781 cgcgccgaga taatcatccg gagtcaggat ggtcgcgcga atccacggct cttcaatctc
      841 ttcaatcttg gtcggatcgg gcatatcggc cggattgtgc aattccaaat tcccgccgcc
      901 tcgcagcacg attcgataga taacggaagg cgccgtcgct atgagatcga gattaaactc
      961 cctctcaagt cgctctcgaa tgatctccag atgcaagagc cccaaaaatc cgcagcggaa
     1021 accaaatccc agtgctgctg aactttccat ctcgtaggtg aagcttgcat cattcagccg
     1081 caagcgtccc attgccgagc gcagatcccc aaaatccgca gcatcgtttg ggaaaagccc
     1141 gcagaacacc acgggctggg ccgctttgaa gccagccagc ggctgaacgc aaggatgccg
     1201 cacgtcagtg attgtatctc caacccgagt gtcggcaacc tccttgatct gcgcggtgat
     1261 gaaaccgatt tcgcccggcc cgagctcggc cgcatcctgc atctttggcc ggaatacccc
     1321 gactttctca atctcgtatt gcgcgcccgt tcccatcatt ttgattcttt gatgtttctt
     1381 gagcatccca tcaatgactc gcacgagcac gacgacaccg agataggcat cataccaaga
     1441 atcgaccaaa agcgccttta agggcgcggt ttcatcgccc ctcggcggcg gcaagcgccc
     1501 gacaatcgcc tcgagaacct tgccgatccc ctcccctgtc ttcgccgaga tgagcacagc
     1561 ctgcgacgcg tcgagtccga tcacatcctc gatctgctgc cggatgcgct cgggctcagc
     1621 agcgggcaga tcaatcttgt tcaaaaccgg tacgatctca tgacccgcat cggtcgcgtg
     1681 atagacattg gcgagcgtct gcgcctcgac cccttggctc gcatccacca ccagaagtga
     1741 cccttcgcag gcggcgagcg accgcgacac ctcataggcg aaatcgacgt ggcccggcgt
     1801 atccatcagg tttaggatat agatctcgcc gtcttgagct ttgtattcca accggacggt
     1861 ttgggctttt atggtaatgc cgcgctcgcg ctcgatttcc atggaatcga gcacttgatc
     1921 gaccatttct cgctcggcaa gcgtgcccgt cgactggatc agcctgtcag cgagcgtgga
     1981 tttgccgtgg tcgatatggg cgacaatgga aaaattgcgg aggtttttta agacgtgaga
     2041 cgtcatgcgg gccgagatag caggcgcccg ccgcaaagga aagatgaacc tggaaattgc
     2101 actgcgtccg cctgaaactt gagtgagctt ccgtcgccaa atcaggccgc cctgtaccaa
     2161 ggccagctgt cgcgggcacg cgctactgtc tgatatagaa tatctggcag tgtgcaattg
     2221 cgtggagcgg ttctagaaac ctcgccaatt tgggttattt ctgattccat cgtgacggct
     2281 gggcgggaga caattggaag gcagcctgga ttttttgatg tcgatgatcg attgaagcgg
     2341 ctgagcgatc ttggcgacca actcgaggcg ttccgggcgg ttgtggattt cgagatattc
     2401 cgcccggaac tgaaagcggc gctcgcctat tcggacggcg tgcgaggagg aggtcggcca
     2461 ttcgatcctg ggatgatgct caagattttg gccattcaga cgaccaatac gcgctccgac
     2521 gaacgcacgg agtttctgat caatgatcgc ctgtcgttta tgcgttttct tagacttaaa
     2581 ttgtcggacc gcgtgcccga cgctcgcacg atttgggtat tccgggagaa actcacaaag
     2641 gccggcgccg tcgggccatt gttcgagcgc ttcgatgtaa cgcttcggca atccggttac
     2701 atcgccatgt cgggccagat tgtcgacgcg agcctgatcg cggcgccgcg ccgacgtgtt
     2761 ctttcctgat aagttacgat tttaccggag gcgcaccgcg tgctgaagcc gggcgaaaat
     2821 tcttgttcaa tatcctggga ccgaatttcc gaaaacgagt tcgccgcagt tgtcgccgaa
     2881 acgcttgctg cgctttttcc acaagatcca cccgagtttt tgattcgtat cccttatggc
     2941 tatttcgatg tcaaaagcat tcacgatgag ctttcgaggg cagggtttac gtcaaacgaa
     3001 gcgatttcgc ttgccagcgt taccagggca tcgtcgccgc atgatgctgc catcgcctat
     3061 tgccacggaa caccgctaag aaatgaaatt gaggctcgcg atctgtcacg cgtcgctgaa
     3121 gctacgcaag cggtcgcgga agcactggcg agtcgctttg ggaacggccc acgctgagac
     3181 gcgccggctg ccgacggaat ccaaagtacc accgcgcatc ggaatggcct ttcgcatagt
     3241 cggcactgcc ggtttccggc ccatttgtta gatgtgccaa agttgtctcc gcttactccc
     3301 agtcgcacgc ataatgctgt gctatcttag gcaacacctt taatcacgcg tttcgaatat
     3361 cagcgcggcc tccaagcgcc aaaaatcatc gcccgcgcgg atgcgctgca cgataggatt
     3421 cgagcagcct tgtgctatcg accgccgtat agagctgcgt ggtcgagagc gaggcgtggc
     3481 ccaagagttc ttggatggaa cgcaaatcgc cgccacgctc caaaagatgc gtcgcaaagg
     3541 aatggcgcag agcgtgtggg gtcgcgctat ccgggaggcc aagcgcgccc cgcagccgcg
     3601 caaccgcaag ctggatgatg cgtgacgaga gcggcccacc cttaacacca acaaacagcg
     3661 gtccgtcagg cacaagctga tatgggcaaa gcgcaaggta agcttcgact gcttgcctga
     3721 ccggtgcgat gatcggaacg gtgcgagtct tttgcccctt gcccgtcaca gtcaaaaagt
     3781 cgcgcccggc gaccggcgcc tcggatcgct taacgcacaa gccttctgaa atgcgcaagc
     3841 cagcgccata gagcaaacat aagacggcgc tatcgcgagc gacaacccat tggcactgat
     3901 tttctcctgc gctggcgtcg gctagtatca cctggcgcgc agcggcaatg gtcagcggtt
     3961 tgggcaggct gcgcttgacc cttggcgcct tcagcgaggc aaaggccact gacttgcctt
     4021 ttccgttcct ctcaaggtag cgtgcaaacg agcgcaatcc tgctagttgg cgcagaagcg
     4081 tacggttgcc cgcacc
//
LOCUS       DATMPU010000040         2485 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_957975,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000040 DATMPU010000000
VERSION     DATMPU010000040.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 2485)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2485)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2485
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_957975"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..323)
                     /locus_tag="VNY06_00880"
     CDS             complement(<1..323)
                     /locus_tag="VNY06_00880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012590709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX13427.1"
                     /translation="MLARCSFLPVGIAAAVAFSVLTLPEESLAQDVRHMCSEKYRAAK
                     AEGSLGDETWPQFYSRCTAEMKANPPAATTPPAAEAAPAPLDIRHICSEKYQAAKAAD
                     TLGGE"
     gene            complement(761..1684)
                     /gene="tsf"
                     /locus_tag="VNY06_00885"
     CDS             complement(761..1684)
                     /gene="tsf"
                     /locus_tag="VNY06_00885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014892610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HWX13428.1"
                     /translation="MTNISAAMVKDLREKTGAGMMDCKNALGETGGEIEAAIDWLRKK
                     GLSKAAKKSGRVAAEGLVAVAVRGLDGVAVELNSETDFVARNADFQALAKNIAEVALK
                     TGTADVEALKAAEYPGGGTVGDAIANAIATVGENMTLRRAAALHVGQGFVGHYVHNQI
                     SEDLGKIGVAVALESNADAQTLAPLARLMALHIAAANPLAIDSASLDPAAIARERAVL
                     EGKNEGKPAHVLEKIVDSGLKTYFKEVCLLDQPSIHAEHENKTIGQAVAEVAKTAGTP
                     ISVKAFVRYALGEGIEKKEADFAAEVAAARG"
     gene            complement(1796..>2485)
                     /gene="rpsB"
                     /locus_tag="VNY06_00890"
     CDS             complement(1796..>2485)
                     /gene="rpsB"
                     /locus_tag="VNY06_00890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012590711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HWX13429.1"
                     /translation="TLGGGAAGLTKKERLMISRERDKLEKALGGIKDMGGTPDLIFVI
                     DTNKEQLAIKEAQRLRIPVVAVLDSNSDPDGITFPVPGNDDAGRAISLYCDLAARAAI
                     DGISRAQGSAGFDMGEMEEPSAEALPAADGAVGAVGADERAASDAPAGPGHVLEILTA
                     PRGAPDDLAKLPGVGPQIVKKLNDAGIFHYWQIAAMSPEDAAKIDRMLKLAGRIERDG
                     WIALARSLVAG"
BASE COUNT          514 a          780 c          668 g          523 t
ORIGIN      
        1 ctctcgccgc cgagcgtgtc cgctgccttg gcggcctgat atttttcgct gcatatatgg
       61 cgtatgtcca aaggcgcagg tgccgcctcc gccgccggcg gggttgttgc ggcaggcggg
      121 ttcgccttca tttccgccgt acaccggcta taaaattgcg gccaagtctc atcccccagc
      181 gatccctctg ccttggcggc tcggtacttt tcactgcaca tgtggcggac atcttgggcc
      241 agactttcct ctggcagagt caaaacggag aaggccacag ccgctgcaat tccgaccggc
      301 aaaaaggaac agcgagcaag catgcgaatt ctccttgtat gcaatgcggt agcgtcgctt
      361 cataagccgc gaaatcggcc ataagtcaaa cataaacaat aggcacgccc gccggctctt
      421 agcctagatc ttccgcaacc gcgtaccggg acggttgtat gcaaaggctg ttgtgtccat
      481 tgaaatctcc ggaaacactt ttgctccagt caagagcctc ccaagccgtt gtcaaataca
      541 acggtcgacc cgcaatgcct gaccagacca taacgcgccg attttcaatt tgctcaatgg
      601 ctgcgccatc gagtgaggca cagctggtta agacgagaac agccgagaga acgatggacc
      661 ggtaaacgaa catgcctgta tagcctatac agcgcttcat ttacccgaaa attgggtcaa
      721 gcgcgcggca gggcaacgcg cgtagtataa ggggtcagac ttagccccgc gcggcggcaa
      781 cttcggcggc gaaatcagct tcctttttct caatgccctc tcccagggca tagcgcacaa
      841 aggccttcac actgatcggg gtcccagcgg tcttggcaac ttccgcgaca gcctggccta
      901 tcgtcttgtt ttcatgctca gcatggatcg agggctgatc caggaggcac acttccttaa
      961 aataggtttt tagtcccgaa tcgacgatct tttcgagcac atgcgcgggc ttgccctcgt
     1021 tcttgccttc gagcaccgcc ctttcgcgcg cgatcgctgc gggatcaaga ctggccgaat
     1081 cgatggcaag cggattggcc gcggcgatat gcagcgccat caatcgtgca agcggtgcca
     1141 gcgtctgggc atcggcattc gattcaaggg cgacggcaac gccaattttt cccaaatctt
     1201 cggaaatttg gttatgcaca tagtggccaa caaagccttg gccaacatgc aaagcagcag
     1261 cacgtcgcaa agtcatgttt tcgccgacgg tcgcgatagc attggcgatc gcatcgccga
     1321 ctgtcccgcc gcctggatat tcggcagctt taagagcctc gacatcggcc gtgccagttt
     1381 taagagcgac ctctgctatg tttttcgcga gcgcctggaa atctgcgttc cgggcaacga
     1441 aatcggtctc cgaattaagc tcgacagcga cgccgtcaag gccacgcacc gcaacggcga
     1501 cgagtccctc ggccgcgacc cggccggatt ttttggctgc cttggaaagc cccttcttgc
     1561 gcagccagtc gatcgccgcc tcaatttcgc ccccagtttc gccgagggca ttcttgcaat
     1621 ccatcatgcc ggctccggtc ttctcgcgaa gatccttcac cattgccgca gagatgtttg
     1681 tcatattgcc cttcctttat ggcaggcgaa ccaatgcccg accgaccccg gggcggcgaa
     1741 cctaaagcct gcgaactcca aaaacatttg cccttaatcg gccggacaag cgcattcaac
     1801 ccgcaacgag actgcgtgcg agcgcgatcc aaccgtcgcg ttcgatgcgc ccggcgagct
     1861 tgagcatgcg gtcgatcttg gcagcatctt ccggactcat ggcggcgatc tgccagtaat
     1921 gaaaaatacc ggcatcattg agcttcttga cgatttgcgg ccctacgccc gggagcttgg
     1981 cgagatcatc tggcgcgccg cgcggcgcgg tcaggatctc taaaacgtgc cccggccccg
     2041 caggagcatc tgaggcggcc ctttcgtctg cgccaaccgc cccgactgca ccatccgcgg
     2101 cgggaagcgc ctcagcggaa ggctcctcca tctcacccat atcaaaacca gctgaaccct
     2161 gggcgcgcga aattccgtcg attgccgcgc gcgcggcaag atcgcaatag agggaaatcg
     2221 cccggccggc atcgtcattt ccgggtacag gaaaagtgat tccatcggga tcgctattgg
     2281 agtcgagcac agccacaacc ggtatgcgca atcgctgcgc ttccttgatc gccaattgct
     2341 ccttatttgt atcgatcaca aagatcagat cgggggtgcc ccccatgtcc ttgatgccgc
     2401 cgagcgcctt ttcgagcttg tcgcgctcgc gcgaaatcat cagccgctct ttcttggtca
     2461 atccggctgc gccgccccct agcgt
//
LOCUS       DATMPU010000041         4669 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_30571, whole
            genome shotgun sequence.
ACCESSION   DATMPU010000041 DATMPU010000000
VERSION     DATMPU010000041.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 4669)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4669)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4669
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_30571"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(290..463)
                     /locus_tag="VNY06_00895"
     CDS             complement(290..463)
                     /locus_tag="VNY06_00895"
                     /inference="COORDINATES: protein motif:HMM:NF017206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HWX13430.1"
                     /translation="MRVLVAGATGALGRRLVPLLIAAGYSVAGLTRSPAKLSLLQRMG
                     AERSSPMHLIPRL"
     gene            667..1128
                     /gene="queF"
                     /locus_tag="VNY06_00900"
     CDS             667..1128
                     /gene="queF"
                     /locus_tag="VNY06_00900"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003613903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preQ(1) synthase"
                     /protein_id="HWX13431.1"
                     /translation="MVKVHTGLSLLGRAADGPRNPEEATLDRVPNPHQGENYIARFTV
                     PEFTSLCPVTGQPDFALLVIDYLPGEWLVESKSLKLYLTSFRNHGAFHEDCTLRIGKD
                     LAACLKPRWLRIGGYWFPRGGIPIDVFWSTGELPTGTWVPDQGVQSYRGRG"
     gene            complement(1139..2767)
                     /locus_tag="VNY06_00905"
     CDS             complement(1139..2767)
                     /locus_tag="VNY06_00905"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011158435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HWX13432.1"
                     /translation="MARFIFITGGVVSSLGKGLAAAALGALLQARGYNVRLRKLDPYL
                     NVDPGTMSPYQHGEVFVTEDGAETDLDLGHYERFTGRPALREDNITTGRIYQEIIARE
                     RRGDYLGATVQVIPHVTNAIKDFVLSGNEGVDFVLIEIGGTVGDIEGLPFFEAIRQLG
                     NELPRGHAVYVHLTLLPFITSSGELKTKPTQHSVKELRSIGIQPHVLLCRTDRTIPPD
                     ERRKISLFCNVREAAVIEARDVASIYEVPHAYHAAGLDREVLAAFAIEPAPKPYMGRW
                     NAVMERVTNPEGEITIAIVGKYTGLKDTYKSLNEALCHGGMANLVRVNLDWIESEIFE
                     SSDPTPYLEHVHGILVPGGFGQRGAEGKIRAAGFARERKVPYFGICFGMQTAVIEAAR
                     SLAGVADASSTEFGPATQPVVGLMTEWMRGNELEIRAAKGDLGGTMRLGAYRAELLPG
                     SKISAIYGTTSISERHRHRYEVNTAYRKRLEEKGLVFSGMSPDGLLPEAIEFSDHPWF
                     IGVQFHPELKSRPFEPHPLFASFIAAAVEQSRLV"
     gene            complement(2847..3440)
                     /gene="secG"
                     /locus_tag="VNY06_00910"
     CDS             complement(2847..3440)
                     /gene="secG"
                     /locus_tag="VNY06_00910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015339948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HWX13433.1"
                     /translation="MQSVLIVIHLMIVVVLVAVVLLQRSEGGALGVGGGGGFLTGRSQ
                     ANALTRATAILAALFFATSLGLTFLARLNQGPEATFQSITPVEPGGGGTAKEGEGKAS
                     LLDQLKRMEDQSARPAGLPPGVSLPGQAAPAAPQGTPSVLETKPAAPEGLKLPDQKRP
                     PTPLSTLAPGQPAPATQGQTGPLPLPVKPEKPGLAPP"
     gene            3999..4436
                     /locus_tag="VNY06_00915"
     CDS             3999..4436
                     /locus_tag="VNY06_00915"
                     /inference="COORDINATES: protein motif:HMM:NF022464.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2849 domain-containing protein"
                     /protein_id="HWX13434.1"
                     /translation="MPKVISANRLTDGFVVYAGQGGAWFERLDEARIFASKEDADAGL
                     SLAQDDVTRNLIVEPCLVDVTRDAGGLRPSTLRESIRARGPTIDFLPHRRAITSDAIP
                     PHKSPGKQAAKPVLRTAEADDLRAFGPKAREAKSVATLADGIG"
BASE COUNT         1002 a         1391 c         1327 g          949 t
ORIGIN      
        1 tccatagcgc aaaacaatcc cttctaagag cgcttcggag atgaccgcag attcgagata
       61 tcggatcgcc tcgagggtat ggcgaaacgt tggcggcgga ttggaaagct ttgcgcgacg
      121 aaacggcgca cccctgcggc acgcgcggcc gtcagcaaat tatccgtgcc ctcttttcgc
      181 agccggttgg tcgcggcgaa tcccccatcg attttcgccg gtcgatcttc ccgatagaag
      241 tgagctcgtg aataataatt tgcggccttg tttgccagac cgcatttttt tatagccgag
      301 ggatcaagtg catcggcgac gatcgctcgg cacccatcct ctgcaaaaga ctcaacttgg
      361 caggagagcg cgtcaaaccg gcgacgctgt accccgccgc aatcagtaaa ggaacaaggc
      421 gccggccgag ggctccagtt gctccggcaa cgagaactct cattgaagtg aaacaacccc
      481 gacaaaacta aaggaacagt tggagaatca acggcggcgg gcgaacatgg ttccaggaat
      541 cccgtggcct ccagaaagcg accaaatatt tggccccgcg gcctcacgac tcgtgagata
      601 atacgatagc cgcacatttt ggtaaaaaat attttggcgg caggagctgt ttctggagca
      661 gaccgcatgg tcaaggttca cactgggcta agcttgctcg gccgcgcggc ggatggcccg
      721 cgcaatcccg aagaagcgac gctcgatcgc gtaccaaatc cgcatcaagg cgaaaactac
      781 atcgcccgct ttaccgttcc cgaattcacc tcgctctgcc ctgtcaccgg ccagcctgac
      841 ttcgcgctcc tcgtgatcga ttatcttccg ggcgaatggc ttgttgaatc aaaatcccta
      901 aaactctatc tgacctcctt tcgtaatcac ggcgcctttc atgaggactg cacgttgcgg
      961 atcggcaagg acctcgcggc gtgcctcaag ccgcgctggc tgcggatcgg cggctattgg
     1021 tttccgcggg gcggcatacc cattgacgtg ttttggtcaa ccggcgagtt gcccaccggt
     1081 acttgggttc ccgatcaagg ggtgcagtcc tatcgcggca gggggtagga cgagtgactt
     1141 aaaccagccg cgattgctcc accgccgcgg caataaagct cgcaaagagc ggatgaggct
     1201 cgaaaggccg cgatttgagc tcgggatgaa actgcacccc gatgaaccaa ggatgatcag
     1261 agaattcgat cgcctccggc aagagcccgt ccggggacat gcctgagaaa acaagtccct
     1321 tttcttctaa ccttttccga taggcggtgt tgacctcata gcgatgccgg tgacgctcgg
     1381 atatgctcgt cgtgccatag atcgcagaga tcttcgatcc tggcaacagc tctgcgcgat
     1441 aagcgccaag ccgcatcgtt ccgccgagat cgcctttggc ggcgcgtatt tcgagctcat
     1501 tgccgcgcat ccattcggtc atgagtccaa caactggctg tgttgcggga ccaaattccg
     1561 ttgagctggc atccgctacg ccggccaagg agcgcgcagc ttcgatcacg gccgtctgca
     1621 ttccaaagca gatgccgaaa tagggtactt tgcgctcgcg agcgaaaccc gcggcgcgaa
     1681 ttttgccttc cgcgccgcgc tggccaaagc cgcccggaac caaaatccca tgcacatgtt
     1741 cgagataagg cgtcggatca ctcgactcga aaatttccga ctcaatccaa tcgaggttga
     1801 ccctgaccag attggccatg ccgccatggc acagcgcttc gttcaaggac ttataggtat
     1861 ccttaaggcc agtatatttg cccacaatcg cgatcgtgat ctcgccttcc gggttggtga
     1921 cgcgctccat caccgcgttc cagcggccca tgtatggctt cggcgcgggt tcaattgcaa
     1981 aggcagccag cacctcccga tcgagccccg ctgcatgata ggcgtgcggc acctcgtaga
     2041 ttgaggccac atcgcgggct tcgatcaccg ctgcttcgcg cacattgcaa aaaagactga
     2101 ttttgcgccg ctcatcgggc gggatcgtgc gatcggtgcg gcaaagaagg acatgcggct
     2161 ggatgccaat tgagcgcaat tccttgaccg aatgttgcgt cggtttggtc ttgagctcac
     2221 ccgagctggt gatgaacggg agaagggtga gatggacata gaccgcatgg ccgcgcggca
     2281 attcattgcc gagctggcga atcgcctcga agaatggaag accttctata tccccgaccg
     2341 tgccgccaat ctcgatcagg acaaaatcga cgccttcatt tccgctcagc acaaagtcct
     2401 tgatcgcatt ggtgacatgc ggaatgacct ggacggtcgc accgaggtaa tcgccccggc
     2461 gttccctggc gatgatctcc tgatagatcc ggccggtcgt aatgttatcc tcgcgcagcg
     2521 ccggccggcc ggtgaagcgt tcataatgac cgagatcgag atcggtctcc gccccatcct
     2581 cggtcacgaa gacttcgcca tgctggtagg gactcatcgt ccccggatcg acattgagat
     2641 aagggtcgag cttgcgcagc cgcacattat agccgcgcgc ctgaagcaac gcgcccagtg
     2701 ccgccgccgc gagtcccttg ccaagcgatg aaaccacgcc gccggtaatg aagataaacc
     2761 gcgccatggg ccttggggtt tatcttgttt ccgcctgctt ggggaaggaa attatgcgaa
     2821 gcttcccctt atgcacataa acccagtcac ggcggggcta acccaggctt ctccggcttc
     2881 actggcagag gcagaggacc ggtctgacct tgggtggcgg gagcgggttg acccggcgca
     2941 agcgtcgaga ggggcgtcgg ggggcgcttt tgatcaggca gtttcagccc ttccggtgca
     3001 gctggtttgg tttcgaggac gcttggcgtt ccttgcggcg cggccggtgc ggcctgaccg
     3061 ggcaagctca cgcccggagg cagccccgcc ggccgggccg attggtcttc cattcgtttc
     3121 agctgatcga gcaagctggc cttgccttca ccctctttcg ctgttccgcc gcctcccggt
     3181 tccaccggag tgatgctttg gaaggtcgct tccgggcctt ggttcagccg cgcaagaaat
     3241 gtcaggccta ggctcgttgc gaagaacagt gccgccaaaa tcgcagtcgc tcgagtcaag
     3301 gcgttcgcct ggcttcgtcc ggtcaaaaaa ccgccgccgc ccccgacccc aagcgctccg
     3361 ccctcggaac gctggaggag cacaaccgcg acgagcacaa ccacgatcat gagatgaatg
     3421 acgatcagca ccgattgcat gaaaattttc tctttgagcc caggggttcc ccgctccgcc
     3481 ccgctgaagc acaggtaccc gattttactt ttagacgtct cctgccgtcg tggctagatc
     3541 ataatctaac ctacgacggg aaagcgccag ctttggccag cgtcgttaca gacggctgcc
     3601 caggcgcgcc ttttggtttt gcgccccctc caagccgatc gtcgggcaaa ttccggggaa
     3661 atcagggcaa cggagaccat ggggccaagc tataacgtag gcgcgacggg gtggcaaaat
     3721 ttttagggca ttgcaatctg gctggcctgc gccacacccg ccgcagctat gatttaccat
     3781 gaatagggca gcgaagttac ctcctgccgc aaattcacca cacatgtggc aattgcgcca
     3841 caacagttaa acccacaaac gtcagcgatc ttttaaggac tatcgacggc ggggaatcat
     3901 atttacaaat cttttttgtg tatttccgca aacactcgac aaactctgtt aattagccta
     3961 tatcctggca aactctgttg tacatagagc ctcgccacat gcccaaggtt atctccgcaa
     4021 accgcctcac tgatggattc gtggtctatg ccggccaggg cggagcttgg tttgagcggc
     4081 tcgatgaggc gcgtattttt gccagcaaag aagacgcaga cgcggggctg agccttgccc
     4141 aggatgatgt cacgcgtaat cttattgtcg aaccttgtct cgtggatgtc acgcgggatg
     4201 caggcggtct acgaccgtcg acgctgcgcg aatccattcg cgcgcgaggc ccaacgatag
     4261 atttcttgcc gcatcggcgc gccatcacat ctgacgcgat tcccccgcac aaaagccccg
     4321 gcaagcaagc cgcgaaaccg gtgttgcgca ccgcagaggc cgatgacctg cgagcctttg
     4381 gacccaaggc gcgtgaagct aaatctgtcg ctactcttgc agacggaatc gggtaatgaa
     4441 acggcgcaga aataggccaa cggaaaggcg gcgggggcgc gggccagcgc accggggccg
     4501 agcgatctaa cgagcgatat aaaatgtacc gctacgatca attcgacaga agctttgtcg
     4561 cggcgcgcgc ggcacagttt cgtgatcagg tggcgcgccg gctatcagga gaactgaacg
     4621 aggagcagtt caaaccgctt cgccttatga atggcgtcta tctgcaact
//
LOCUS       DATMPU010000042         5143 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_338420,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000042 DATMPU010000000
VERSION     DATMPU010000042.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 5143)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5143)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5143
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_338420"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            132..1496
                     /locus_tag="VNY06_00920"
     CDS             132..1496
                     /locus_tag="VNY06_00920"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HWX13435.1"
                     /translation="MTACPTDLPRPPAPVPLDAPLGPLSTIRVLWRNPIETWTKAHFE
                     LPISIGHGVLGKVAVVSEPGAIRRVLVDNAPNYRKDAIQKRLLGPGLRHGLLEAEGDD
                     WRVQRRTLAPLFKPKIVACFAPAFNAAAEELVARLERLREGQIVDIQHEMALVTLDVL
                     GRTIFSDGLGRDAKEVASAISRLLATVGRIDPFDILNFPEWVPRFSKIGGGAAQNFFD
                     SMIDSLIAKRKNLLTFDGASAPRDVLTLLLEAQDPETSAGLSDTQVRANILSFIAAGH
                     ETTANALTWSIFLLSLSKEWREALAQEASEVLPHPVEDYAQRLVKTKAVIEEAMRLYP
                     PAASISRQAEGVDDLAGRHIQMNSLVIISQWVLHRHQLLWENPDRFDPRRFLPGARER
                     IDRFAYLPFGVGPRVCIGASFSMQEAAIILAHLMRSFALSFKTTIRSCRCNASPCAQR
                     AGCQ"
     gene            complement(1633..2706)
                     /locus_tag="VNY06_00925"
     CDS             complement(1633..2706)
                     /locus_tag="VNY06_00925"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HWX13436.1"
                     /translation="MEGSLSRFTTRAVYGARQLSRIGWYLGHGVALRRFSDEVRRREG
                     ESIRPRPRTNGAVPGRGRLYADMAALFQRDLANVEAGIYPLPEDNDGSLLTRLNRSRL
                     FFEDVPDVHRRRESGQGKEVLTPETSGKRPLYYLQNFHFQSGGYLTDESARRYDTQVE
                     VLFSGTANATRRQALPQIHEVLAGRDQRSLKLLDVGCGTGRFLDFVKQAWPRLPAVGL
                     DLSEAYLKEAQRHLERRSRINLVVGNSESLPLPDESQDVVTSIFTFHELPPEARRNSF
                     REFARVMKPNGRLVIVDSIQRGDKPDYEGLLELFPQSFHEPYYLSYVDEDFAAIGRTC
                     GLMHRRDTDAFMSKVMVFDKLVG"
     gene            complement(3050..3583)
                     /gene="thpR"
                     /locus_tag="VNY06_00930"
     CDS             complement(3050..3583)
                     /gene="thpR"
                     /locus_tag="VNY06_00930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012383839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HWX13437.1"
                     /translation="MPRLFTGLEIPSDLAMDLSMLRGGLSGARWIDAENYHLTLRFIG
                     DIDDATAREIHSTLERIRLPPFTVTIEGLSSFGGTKPRALIAKAKSAPVLVELQAQQE
                     RLIRRTGVPPDGRKFIPHVTLARLRMASCAAVAEYLSARGGFFSRHFEATRFVLFSSR
                     ASTGGGPYIVEAAYPLG"
     gene            complement(3693..4433)
                     /locus_tag="VNY06_00935"
     CDS             complement(3693..4433)
                     /locus_tag="VNY06_00935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012456180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylesterase"
                     /protein_id="HWX13438.1"
                     /translation="MTSEITRKYLLLNRMPWPNFDGYGSRRTFLQMGLAAVLLAPGSS
                     CPASSAETETSPKKIIAFGDSLTAGLGLPSDVAFPAVLEKALRTEGYNVTIVNAGVSG
                     ETASGGLARLDWTIGDGADGVILELGANDMLRGINPEVTEAALDAVLAKLKVRKIKVM
                     IAGMKATPSLGPEYKSRFDAIYPALAQKYDAPLYPFLLDGVAGVPALKQNDGLHPNSV
                     GVAHIVKGIMPTVRAFLDQLGTKAARQE"
     gene            4436..5122
                     /locus_tag="VNY06_00940"
     CDS             4436..5122
                     /locus_tag="VNY06_00940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009493660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWX13439.1"
                     /translation="MEEPAVELDNVHLSLGRGAARVHILKGISLTIPPGETAGLIGPS
                     GSGKSTLLMVIAGLEQPDTGTVKVDGVNLGKLGEDALARFRGARIGIVFQSFHLIPTM
                     SALENVAIPLELSGAVAPFERARVELAAVGLKDRLSHYPAQLSGGEQQRVAIARAMAP
                     GPAILVADEPTGNLDEATGASIIDLIFALSRDRGTTLVVVTHDLALAGRCDRRIWLHS
                     GRIEEAAATV"
BASE COUNT         1065 a         1515 c         1489 g         1074 t
ORIGIN      
        1 caacgcgctt tccaagttat gcgaaatcca acagccgcgg ttccaaatag cgatgaccaa
       61 agagcccaga atataagcat actgagcatt tggaactccg gcaacttcca taacatgtac
      121 aatgggtaat catgacagcc tgcccgaccg atttgccgcg cccgcccgca cccgtgcccc
      181 tcgatgcccc gcttggccct ttgtcgacca taagggtctt gtggcgcaat ccgatcgaga
      241 cctggaccaa ggcccatttc gaattgccga tttccatcgg ccacggcgtc ttgggcaagg
      301 tcgcagttgt cagcgagccg ggggcaatcc gccgggtgct cgtcgacaac gcgccgaatt
      361 accgtaagga tgcgatccaa aagcgcctgc ttgggccggg cttgcggcac ggcctcctcg
      421 aagcggaagg tgacgattgg cgggtccagc gacgcacgct cgcgcccttg ttcaagccta
      481 aaattgtcgc ctgctttgcg cccgcgttca atgctgcagc cgaggaactg gttgcccggc
      541 tcgagcgtct gcgcgagggg cagatcgtcg acattcaaca tgaaatggct ctcgttacgc
      601 tcgatgttct cggccgcact atcttttccg acggtctcgg ccgggacgcg aaggaagtcg
      661 cgtcggctat ctcccggctc cttgccacag tcggccgcat cgacccgttc gatattctga
      721 atttcccgga gtgggtgccg cgcttcagca aaatcggcgg cggggccgcg cagaattttt
      781 ttgactccat gatcgactca ctcatcgcca agcgtaaaaa tctcttgacc ttcgatggag
      841 ccagcgcgcc gcgcgacgtg ctcaccctcc ttctagaagc ccaggatcca gaaaccagcg
      901 cggggctgag tgatacacaa gtccgagcca atattctgag cttcatcgct gctggacacg
      961 agacgaccgc caacgccttg acgtggtcga tctttcttct ctcgctctcc aaggaatggc
     1021 gggaagccct ggcgcaggag gcgagcgagg ttttgcctca ccccgtcgag gactacgctc
     1081 agcgtctcgt caagacaaag gcggtgatcg aggaagccat gcgcctttac ccgcccgcag
     1141 ccagcataag ccgccaggcg gagggagtag acgatcttgc cggcaggcat atccaaatga
     1201 acagtcttgt cattatatcg cagtgggtcc tgcaccggca ccagttactt tgggaaaacc
     1261 cagaccgttt cgatccgaga cgattcctgc cgggggcgcg cgagaggatc gatcggttcg
     1321 cttatctgcc ctttggcgtc ggaccccgcg tctgtatcgg cgcatccttc tcaatgcagg
     1381 aggcggccat cattctcgcc cacctcatgc ggtcgtttgc tttgagcttc aaaacaacca
     1441 taaggtcatg ccggtgcaac gcatcacctt gcgcccaaag ggcgggctgc caatgatttt
     1501 gtcgcggcgg cgtccttatt gaacctcagt gcccaggtcc tttgtccgtc aaatgatttg
     1561 attgccgcga tgatagaagg gagttgcgtg tccaattcgc gaatctttga actcgagtcg
     1621 ggcgggcggc gatcaaccta ccagcttatc aaacaccatc accttggaca tgaaagcatc
     1681 cgtgtcgcgc ctgtgcatca aaccgcaagt cctgccgata gcagcgaagt cctcgtcgac
     1741 ataactgaga taatagggct cgtgaaaact ctgcggaaag agttcgagta gcccttcata
     1801 gtcaggcttg tcaccccgct ggatcgaatc cacaatgacg agacgcccat tgggcttcat
     1861 tacgcgagcg aactcacgga agctattgcg gcgcgcctca ggcggcagtt catggaaagt
     1921 gaagatgctc gtcactacgt cttggctttc atccggcaaa ggaagcgatt ccgagttgcc
     1981 gacgacgagg ttaatccgcg accggcgctc caaatgacgc tgcgcctcct ttaggtaagc
     2041 ctccgaaaga tcgaggccga cggcgggaag gcgtggccag gcctgcttga caaaatccaa
     2101 aaaacgcccg gtgccgcaac cgacatccag aagcttcagc gaacgctgat cgcgcccggc
     2161 gagaacctcg tggatctgag gtaaggcctg gcgtcgcgtg gcattcgccg tgccactgaa
     2221 cagcacctcg acttgtgtgt catagcgcct ggctgattcg tcggtcagat agccgccgga
     2281 ttggaaatga aaattctgca gatagtagag gggccgcttt ccgctcgtct ccggggttag
     2341 cacttctttg ccttgcccac tctcccggcg gcggtgaacg tcgggcacat cctcaaagaa
     2401 tagccgtgag cggttgagcc gcgtcagaag cgagccgtca ttgtcctccg gcaagggata
     2461 gatgcctgcc tcgacattgg ccaaatcccg ctggaaaaga gcggccatat ccgcgtagag
     2521 gcgcccgcgg ccaggcaccg caccattggt acgagggcga ggccttatgc tctcgccttc
     2581 ccgtcgccgc acttcgtccg aaaaccggcg cagagcaacg ccatggccga ggtaccagcc
     2641 aatgcgggag agctgccttg cgccgtagac tgcgcgtgtg gtgaagcgcg agagcgagcc
     2701 ttccatgttt ttctcttgct tagcaagtaa gctggattga cctaaaatat cgcccgaaat
     2761 ttagacatca agtgggtatg acacacttgt gtcgaagcta tgattttgca gttcgcgcca
     2821 ttatcaccgc gattgcggtg tgatccatag cagcggtggt cttgcgccag acggcgatca
     2881 tccttgtcca tgtctgcgac cggtttcctc tggcttgaga agaaccagct agacatcccg
     2941 agggaggcga ataggcttgt ggcgcgcgtg gccagagcaa gatgactgtt tttcgaatcg
     3001 tcatcttgct ctcactcttt cccatttttg gctcgacatg ctcttgcgtt cagcccagcg
     3061 gataggcggc ttcgactata taggggccgc cgccggttga tgcccgggac gaaaacaaga
     3121 cgaaacgcgt cgcctcgaag tggcgggaaa agaatccccc acgcgcggag agatattcgg
     3181 caacagccgc gcaggatgcc atgcgtagcc gcgcaagggt gacgtgagga atgaatttgc
     3241 gcccatcagg tggaacgcct gtgcgccgta tgagccgttc ttgctgcgct tgaagttcga
     3301 ccaatacggg cgcggacttg gccttcgcga taagcgcccg gggcttcgtg ccgccaaaag
     3361 aactcagacc ttcaatcgtc accgtaaagg gtggcagccg gatccgctcc agcgtcgaat
     3421 ggatctcccg cgcagtggcg tcatcgatat cgccgatgaa acgcagggtc agatggtaat
     3481 tttccgcatc aatccagcgc gcgccggaaa gtccgccgcg caacatggaa agatccatgg
     3541 cgagatcgga agggatttcg agtcccgtga acaaacgggg catggatccc tcctaaccgt
     3601 cggcccggat tctcgaatcg gagcgctaat agatatgatc ataacggaga ttcggcgcat
     3661 tgggaaagct cgaccgcaaa tccttcctca gcttattctt gcctcgcggc ttttgtgccc
     3721 agttggtcca agaaggcccg gaccgtcggc attatgcctt tgacaatgtg ggcgacgccg
     3781 acgctgttcg ggtgcaatcc atcgttttgt ttcaaggcag gcacccctgc tacgccatcg
     3841 aggaggaatg gatagagcgg cgcatcatat ttttgggcaa gtgccggata aatggcatcg
     3901 aagcgcgact tatattcggg gccaaggctt ggcgtggctt tcattcccgc gatcatcacc
     3961 tttattttac gtaccttaag ctttgctaga acggcatcga gcgcggcctc cgtcacctcg
     4021 ggatttatcc cgcgcagcat atcgttggcg ccgagttcca ggatcacgcc atcggcgcca
     4081 tcgccaatgg tccaatcgag cctcgctagg ccgccggaag cggtttcgcc ggagaccccc
     4141 gcgttgacga tggttacatt gtagccttcc gtgcgcagcg ccttttcgag cacggcgggg
     4201 aaggcgacgt cgcttggcag cccgagaccg gcagttaggc tgtcgccaaa agcaatgatc
     4261 ttttttggcg aggtttccgt ttcggcggat gaggcagggc atgatgagcc cggtgccagc
     4321 agcacagccg caagccccat ttgaaggaag gttcggcgac tgccatatcc gtcaaaattc
     4381 ggccatggca ttcgattgag caaaagatac ttgcgtgtga tctcagaggt catgaatgga
     4441 agaaccggcg gttgaactcg ataatgtgca tttaagcctc gggcgcggcg cggcgcgcgt
     4501 tcatattctc aaaggtatct cgctcacgat cccgcccggc gaaaccgccg gtttgattgg
     4561 cccatcgggt tctggcaaat cgaccctgct gatggtgatc gcgggccttg aacagcccga
     4621 caccggcacg gtcaaagtcg atggcgtgaa cctcggcaag cttggcgaag atgcgctcgc
     4681 gcgctttcgt ggcgcgcgga ttggtattgt atttcaatcg ttccatctca ttccaaccat
     4741 gtctgcgctc gaaaatgttg caattccgct cgaactctcg ggcgctgttg caccctttga
     4801 gcgggcccgc gtggaacttg ccgctgttgg cctcaaagat aggctctcgc attatccggc
     4861 gcaattgtcg ggcggcgagc agcaacgggt tgcgatcgcg cgcgcaatgg cgcccggccc
     4921 cgccattctc gtcgccgacg aaccgaccgg aaatctcgac gaggcaaccg gggcaagcat
     4981 catcgatctc atcttcgccc taagccgtga tcgcggcacg accttggtcg tcgtcaccca
     5041 tgatctcgcg cttgccggca gatgcgatcg caggatttgg ctgcactctg ggcgcatcga
     5101 ggaggccgcc gcaacggtat agcggcagca ggcggaaatt tga
//
LOCUS       DATMPU010000043        10886 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_341023,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000043 DATMPU010000000
VERSION     DATMPU010000043.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 10886)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10886)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10886
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_341023"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(120..1532)
                     /locus_tag="VNY06_00945"
     CDS             complement(120..1532)
                     /locus_tag="VNY06_00945"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HWX13440.1"
                     /translation="MSRSAIPKCIHATRRILLPSSLKKGALSFFDTLIMAIAGSAPAY
                     SITVSTAALIVAAGLAGPAALWVAALPMFGITLAFAQLNRWQPDAGAAYVWVGRAMHP
                     ALGFLAGWALLSLSTIFNVAAALPAGQATLEIFAPGLAHDVLWATAVGALWFLGVLAV
                     VTAGITVSSRTQIALTLLEIFAVIAVCGAAIWSARTAPAAAFSWQWFSPTSFGSFQAF
                     SAGMLVALFYYFGWDVSANLAEETADADWAAGLACILGVLIVVALFLLAQVATQFALT
                     HRDIEAHGGNLLPVLGAAGLPAPWSAIAVVAILVSTIATIETQLLQCTRLLFSMARDR
                     VIGESMGELHPRFQTPWLAGIAVAGVTLLLFASSATLPSIGALMTGLINAIGVQVACY
                     YALAGIACAWHFSTHGGWKTRLFAVVIPLTSALFVAGVGIYQLPFLGWRVSALSIGTI
                     LIGIVPFFYYRWRYRGHFLT"
     gene            complement(1625..2470)
                     /locus_tag="VNY06_00950"
     CDS             complement(1625..2470)
                     /locus_tag="VNY06_00950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003571236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HWX13441.1"
                     /translation="MRRFLFLFCFFAIGPTASAEPSPQWLTLPATPTLPQAAKSGYAP
                     LNDIKIWYAEYGRGEPVILLHGGLANANYWGNQVPELAKSYRVIVMDSRGHGRSTRDN
                     RPFGYGLMASDVIALMDYLEVSKAAIVGWSDGAILGLDIAIHHPERITKLFAFAANSD
                     PSAVKDVTQSPVFNNFIARAKAEYEQLSSTPDAYKSFLDQITGMWAAEPNFTAEQLRA
                     ISVPVLIADGDHDEAIKRENAEFMAKEIPNAGLLLQPGVSHFAFLQDPEKFNCDLLHF
                     LQHVR"
     gene            complement(2704..3663)
                     /locus_tag="VNY06_00955"
     CDS             complement(2704..3663)
                     /locus_tag="VNY06_00955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012591210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
                     /protein_id="HWX13442.1"
                     /translation="MAFRQRTLILILAATLTLGFAGDAFARAGGGFSSGSRGARTFSM
                     PNSTPTAPRAAPMERSASPNEGIFRRASPYSQGGFFSSSFGRGLLGGFLGAGLFGLLF
                     GNGLFGGLGGGLSILGLILQIGLLFLLFKLIMGFIRGNRPSFQSAGLGGRPGNGFGQM
                     GASARGAFGGFGGGQAGPRQDTKLEPAEADFAMFEQRLAEIQSAFSAEDVDRLRHLAT
                     PEMASYFTEELAANAGKGVVNKISDVKFLQGDLAEAWCEQNAEFATVAIRFSLIDTMA
                     DRISGKIVSSDPQVPQQVTEVWTFMRPAGGTPADWKLSAIQQA"
     gene            complement(3759..5768)
                     /locus_tag="VNY06_00960"
     CDS             complement(3759..5768)
                     /locus_tag="VNY06_00960"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HWX13443.1"
                     /translation="MKFRYDINALRALAVTAVVLYHYKVNFIPGGFVGVDVFFVISGY
                     LMTTIITERLARGSFGLWDFYNERARRIIPGLLGLCFGLLAAGYFVLEPGAYQILGSE
                     AIGALLFVSNFQFWEGTSYFDPQSVDKWLLHSWSLSVEWQFYLLYPIILLRLHAARIT
                     RRRLTPILWLLILASFLLCVWSASYYPASAFYLLPQRAWEMFAGGITALQFRYSQWKN
                     PSILIGFGLLSIAIALFAYDNAMAWPSYWALLPVIGTCLVIAANQADASLFRNQIVQT
                     LGKWSYSVYLWHWPIAVAAAYFYFVKTTALKIVCEILILTAILAIGAIFLLLIERIWT
                     RLMPERGLRAAAGGAIAMVLIVAFAFRVTNDGLIGRRQDIASELETYAKAAHDWSYPD
                     ECIGTDSAGNLKPCRLGRAKNRGVLFIGDSFAMHIYGRFAKAAKFDPETSFTFLASLG
                     CPPVTGMRIVQDRYNCDGFVDKALQFAKMHKFKRIVLASRWNAYFTPKEGWMCFETSG
                     GCRLEREPSSYYRQLDAALCDLRSRLLELKDRGAEIIILWTTPWGRWNVPSELAKSKF
                     LGLDTRDMEYIDRVEFENNAAPIKGRLMDLASAIGGRFVDPLEFLCDGRRCQTVDQDG
                     VPYFIDDQHIRASMITTARFRFLDDAAGIREQLSAAPAPVNSPKF"
     gene            complement(5880..7985)
                     /locus_tag="VNY06_00965"
     CDS             complement(5880..7985)
                     /locus_tag="VNY06_00965"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HWX13444.1"
                     /translation="MPCFALQLESQPGNRAKDELNEGSMKFRYDINAVRAVAVTAVVL
                     FHYKVDFIPGGFVGVDVFFAISGYLMTSIIMGRLAKGKFSIWEFYHDRAKRIVPGLLG
                     MCFVLLAAGYLFLEPATYHYLGSTSIAALLFFSNFRFWEATGYFEPQSDVKWLLHTWS
                     LSVEWQFYLAYPIILLGLYKFDKTRRHIIPILWSMALLSFLSCVWLSTNGPPSAFYLL
                     PPRAWELIAGGIVALQFANDGRRYSATLLASGLLFIGISIALYDKNLPWPYYWGLLPV
                     VGTCLVIAANRADAPIFKNKLIQTIGVWSYSIYLWHWPVAVAALYLGFATTTPFKIVA
                     EIVIVAAILAVGRLLLSLGRKFIETRLAENRWPELIWGASALGLTLAFAGTITINQGL
                     ANRRPDGDKEYLAYQTALHDWDYPEACNGTDASGNLRPCRLGLGNDRGVLVIGDSFAM
                     QIYSRFAEIVSRDPDSSFTFLASPACPPLTGIRTVHDRFKCNGFVEKAMQFAEARNFA
                     RIMLVSNWYGYFNPQDGWMCFETGGGCRLEREPESFYPLLDAAFAGLRSRLLELKNRG
                     TQIVIVGATPSGRWNVPTELAKRRFLGMDTTDIEYIDRATFDDRAYPVKSRLMALAAS
                     IGEKFVDPLEFLCDQQRCPTVDSEGVPYFKDDCHYRSAAVRTSRFQFLDDVMGPRKRV
                     STAPTIGSSLQSSNLAQAQ"
     gene            8272..8796
                     /gene="fabA"
                     /locus_tag="VNY06_00970"
     CDS             8272..8796
                     /gene="fabA"
                     /locus_tag="VNY06_00970"
                     /EC_number="4.2.1.59"
                     /EC_number="5.3.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012591209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008693 -
                     (3R)-3-hydroxydecanoyl-[acyl-carrier-protein] dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-hydroxydecanoyl-ACP
                     dehydratase/trans-2-decenoyl-ACP isomerase"
                     /protein_id="HWX13445.1"
                     /translation="MKERCAYFEYEDLIACGRGELFGPGNPQLPLPPLLMFDRISDIS
                     EQGGANGKGGVVAEYSVKPDLWFFGCHFKGDPVMPGCLGLDALWQLLGFYLGWLGLTG
                     RGRALGVGEVKFSDQVLPSVKRVTYGVDLKRVFKTKLVLGIADGWLDADGARIYEAKD
                     LRVGLFKATTPSVA"
     gene            8886..10109
                     /gene="fabB"
                     /locus_tag="VNY06_00975"
     CDS             8886..10109
                     /gene="fabB"
                     /locus_tag="VNY06_00975"
                     /EC_number="2.3.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016921727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase I"
                     /protein_id="HWX13446.1"
                     /translation="MRRVVVTGMGIVSSIGNNTQEVLGSLREARSGIVKAEKYAELGF
                     RSQVHGAPALDPEETIDRRAMRFHAGGTGWNHVAMDQAILDAGLSQDEISHERTGIIM
                     GSGGPSTRTIVEAADIGRAKGPKKIGPFAVPKSMSSTASATLATWFKIKGVNYSISSA
                     CATSNHCIGNAYEMIQYGKQDMMFAGGCEELDWTLSVLFDAMGAMSSAFNDRPDAASR
                     AYDMNRDGFVIAGGAGVLVLEELEHARARGAKIYGEIAGYGLTSDGFDMVAPSGEGAV
                     RCMRMALENVETPIDYINPHATSTGIGDLKEIEAVRAVFGSGEKCPPIAATKSLTGHS
                     LGATGVQEAIYSLLMMQNGFICESANIDDLDPAFEDVDIVRKRRDGVQLRAVLSNSFG
                     FGGTNASIVLKHVDA"
     gene            10096..>10886
                     /gene="fabI"
                     /locus_tag="VNY06_00980"
     CDS             10096..>10886
                     /gene="fabI"
                     /locus_tag="VNY06_00980"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013165088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HWX13447.1"
                     /translation="MSTPEARATLAQGGLMRGKRGVVMGVANDHSIAWGIAKALYDHG
                     AELAFTYQGEALGKRVKPLAESLGAPLILPCDVEDAAAIDQLFGSLEEIWGRLDFAVH
                     CIAYSDKAELKGRYSDTTRENFLRTMLISAFSFTEVARRAAALMQPGGALLTLTFEGA
                     SRVMPNYNVMGVAKAALEASVRYLAADFGPHGIRVNAISAGLVRTLAGAGIANGRMIF
                     NFQKAHAPLRRSLDLADMGGAALYLLSDLSSGVTGEIHHVDLGYH"
BASE COUNT         2527 a         3056 c         3131 g         2172 t
ORIGIN      
        1 acaaattggt gagccgtttt tttgttttat tggcgcctgc acagaaatcg aagtgagctg
       61 caattgcatc tgaaagttaa ctcgtaaata ccgcagaagc tggctcaaac cagatgcttt
      121 taggtcagaa aatgtccgcg atagcgccag cggtagtaaa agaatggtac aatgccaatc
      181 agtatcgtgc cgatgctgag cgcagaaacg cgccagccga gaaagggcag ttggtagatc
      241 ccgaccccgg caacaaataa agccgatgtc aacgggatga ccacggcaaa taggcgcgtc
      301 ttccagcctc cgtgcgtgct gaaatgccaa gcgcaagcaa ttccagccaa cgcatagtag
      361 caagcaactt gcacgccgat cgcattgata aggccggtca tcaacgcgcc gatcgacggc
      421 aatgtggccg aacttgcgaa taacaaaagc gtcacgccgg cgaccgcaat gcccgcaagc
      481 catggtgtct gaaaacgcgg atggagctca cccatcgatt ctccaattac cctgtcacgg
      541 gccatggaaa acagcagcct cgtgcattgg agaagctggg tctcgatggt ggcaatggtg
      601 ctgaccaata tcgccaccac agcgattgcg ctccacggtg ccgggagccc cgcggcgccg
      661 aggacaggaa gcaaattgcc gccgtgggcc tcaatgtcgc gatgcgtgag tgcgaactgg
      721 gttgcgacct gcgcaagcag aaacagggcg acgacgatga gcacgccgag gatgcaagcg
      781 aggccggccg cccagtccgc gtccgccgtc tcttccgcga gattggccga tacatcccag
      841 ccaaaataat agaaaagcgc gaccagcatt ccggcggaaa atgcctggaa cgagccgaag
      901 cttgtcggag aaaaccattg ccaggaaaag gcggccgcag gagcggtgcg cgcactccag
      961 atggccgcgc cacatacagc gatgacggcg aaaatttcga gcaaggtcag agcgatctgt
     1021 gtcctggagc ttaccgtaat gccagccgtg acgaccgcca gcacgccgag gaaccacagg
     1081 gccccgacgg ccgttgccca caaaacgtcg tgggcaaggc cgggcgcgaa gatttccagc
     1141 gtcgcttgtc ctgccgggag ggccgcggca acgttgaaaa tcgtcgaaag actaaggagt
     1201 gcccaaccgg cgaggaaacc caacgccgga tgcatcgcgc ggccaaccca gacataagca
     1261 gctccggcgt cgggctgcca ccggttcaat tgagcaaacg caagcgttat gccgaacatg
     1321 ggcagggcgg cgacccaaag tgcggccgga ccagcgagtc cggccgcaac aatcagggcg
     1381 gcagtgctga cggtgatcga ataggcgggc gccgaacccg caatcgccat gatgagcgtg
     1441 tcgaaaaagc tcagcgctcc ctttttcagc gagctaggca acaaaatcct ccgcgtggca
     1501 tgaatacatt tggggatggc agagcgagac atttggtcat gctcgcaatg ctttatgcaa
     1561 tcaaatttat gacgtggctg agaccacggc ggcagatgcg ggcaccgcaa ggccttcaag
     1621 gactctaccg gacgtgttgc aaaaaatgca gaagatcgca attgaacttc tcaggatctt
     1681 gcaagaaagc gaaatgactt acaccgggct ggagcagcaa tccggcattt gggatttctt
     1741 tggccatgaa ttcggcattc tcgcgcttaa tggcctcatc gtgatctccg tccgcgatta
     1801 agacaggtac agaaattgcg cgaagctgct cggcggtaaa atttggctct gctgcccaca
     1861 tccctgtaat ctgatcaagg aaggatttgt aggcgtccgg cgtcgacgag agctgctcat
     1921 attccgcctt cgcgcgcgcg atgaaattat tgaagaccgg gctttgggtg acgtccttga
     1981 ccgcggaggg gtcggagttg gcggcaaagg caaagagttt cgttattcgt tcgggatgat
     2041 ggattgcaat gtctagtccc aagattgccc catcgctcca gccaacgatc gctgccttgc
     2101 taacctccaa atagtccata agcgcgatga cgtcggacgc cataagacca tagccaaagg
     2161 gtcgattgtc gcgcgtgctg cgcccatgcc cacgactgtc catcacgatg acccggtagg
     2221 attttgccaa ctcgggaacc tgattgcccc aatagttcgc gtttgcaaga ccgccatgaa
     2281 gcaggatcac gggctcaccg cgtccatatt ccgcatacca gattttaata tcgttcagcg
     2341 gagcatagcc gctcttcgct gcttgcggca atgttggcgt tgcgggaaga gtaagccatt
     2401 gcggcgatgg ctccgccgaa gccgtgggcc cgatcgcgaa gaaacagaaa agaaacagaa
     2461 agcggcgcat gaatcttcgg tgttggtcag ctataccgac tttgaccggc gcccagactt
     2521 gacgcccgcg cccagaaaag cacgcgaaat caccgccctc gcgaatcttc gagtgaaatg
     2581 cgccaaaatc gtcttgttgc ttccgcgatc ggtaagataa gcggcgagac aacgcttgcg
     2641 gagagttaaa attttcaata gccatcctgg ccggggcgcg ccttacgctc cggcccgtgg
     2701 ctgtcaggct tgctgaattg ccgacagttt ccaatcagca ggagtaccgc ccgccggtct
     2761 cataaatgtc cacacttcgg tcacctgctg cggcacctgc ggatcgctgg agacaatctt
     2821 gccggagatc cgatccgcca tagtgtcgat caaggaaaac cgtatcgcaa ccgtggcaaa
     2881 ttcggcgttc tgctcgcacc aggcctcggc aagatcgcct tgcagaaatt tcacgtcgga
     2941 gattttgttc accacaccct ttccggcgtt ggcggcgagt tcctcggtga aataagaggc
     3001 catttccggt gttgccagat ggcgcagccg atccacatcc tcagcgctaa aagcggattg
     3061 aatctcggca aggcgctgtt cgaacatcgc aaaatcagcc tctgcgggct cgagcttcgt
     3121 gtcttgccgt gggccagctt gcccgccgcc aaagcccccg aaagcgccgc gcgcgcttgc
     3181 gcccatttga ccgaacccgt tgcccggcct tccacccaaa ccagcgcttt ggaaggaggg
     3241 tcggttcccg cgtatgaagc ccatgatgag tttaaagagc aggaacaaaa gcccaatctg
     3301 caggatcagc cccaagatcg acaggccgcc gccaaggccc ccaaagagac catttccaaa
     3361 cagcaagcca aacaggccgg cgcccagaaa acccccaagc agtccgcggc caaagctgct
     3421 cgaaaaaaag ccaccctgag aatagggcga agcgcggcga aaaatgccct cattgggtga
     3481 ggccgacctt tccatagggg ctgcccgagg agcagtcggc gtggaattgg gcatgctaaa
     3541 agtgcgcgcg cctcggctgc cagaggaaaa gccgccgccg gcccgcgcaa aggcgtcccc
     3601 cgcaaagccc agcgtgagcg tggcagcgag gatcaaaatt aaagttcttt ggcgaaacgc
     3661 catcgggtcc atccttaatt cgtgcggaca agccgatccg ccaaatctat atgggcatgc
     3721 gtatcgaaat cgcaaagggc atgggctggt tgcaacggtc agaatttcgg actgttaacc
     3781 ggtgcgggtg cggcgctcaa ctgctccctg atccctgcgg catcgtccag aaatcgaaat
     3841 cgagccgtcg tgatcatgct cgcccgaata tgctggtcgt cgataaaata gggtacgccg
     3901 tcctggtcca ccgtttggca tcggcggccg tcgcaaagaa attcaagcgg atcgacgaat
     3961 cttcctccga tcgcggaggc cagatccatt aaccggccct taatcggcgc ggcgttgttt
     4021 tcaaattcaa ctctatcgat atattccatg tctctcgtgt ccagtcctaa gaatttgctc
     4081 tttgcgagtt cggacggtac attccatcgt ccccatggcg tagtccagag gatgatgatc
     4141 tcggctcccc ggtccttaag ttcgaggagg cgggagcgca aatcgcagag ggcggcatcg
     4201 agttgccggt aataagatga cggctcccgc tccaagcggc agcccccgct tgtctcgaag
     4261 cacatccatc cctcctttgg cgtaaaatag gcattccatc tcgaagcgag cacgattcgc
     4321 ttgaatttgt gcattttagc aaattgcaac gccttatcga cgaagccgtc gcagttatag
     4381 cggtcctgaa ctattcgcat gccggtgact ggcggacagc caagcgatgc cagaaacgta
     4441 aatgacgtct cgggatcaaa tttcgccgct ttcgcaaacc tgccatagat gtgcatggcg
     4501 aaagagtcgc cgatgaagag cacgccgcgg ttctttgctc ggccaaggcg gcaaggtttt
     4561 aaattgccgg ccgaatccgt gcctatgcac tcatccgggt agctccagtc atgggctgct
     4621 ttcgcgtagg tttcgagttc gcttgcaata tcttgccggc ggcctatcaa gccgtcattc
     4681 gttaccctga acgcaaaagc cactatgagt accatggcga ttgcgcctcc cgcagccgct
     4741 cgcagacctc tctccggcat gaggcgcgtc caaatccgct caatcaataa aaggaaaata
     4801 gcgcctattg ctaagatcgc tgtgaggatt aggatttcac acacgatttt gagcgcggtg
     4861 gtcttgacga aataaaagta agccgctgcg accgcgattg gccaatgcca tagatatacc
     4921 gaataggacc atttcccgag tgtctggaca atttggttcc tgaacaaaga ggcgtcagcc
     4981 tggttcgcgg cgatgacgag gcatgtgcca atgacaggca gcaatgccca ataggagggc
     5041 catgccattg cattgtcata ggcaaaaagg gctatggcga tggagagcaa tccgaagcca
     5101 atgagaattg atggattttt ccactggctg taacggaatt ggagcgcggt gatcccgccg
     5161 gcgaacatct cccatgctct ttgaggcaga aggtagaatg cggatgcggg ataatagctc
     5221 gccgaccaca cacaaagcag aaaggatgca agaatcaaaa gccagaggat tggcgtgagg
     5281 cggcgtcgtg ttatccttgc ggcgtgcaag cgaagcagaa tgattggata aagcaggtag
     5341 aactgccatt ccactgagag gctccagcta tgcagaagcc atttgtcgac gctttgcgga
     5401 tcgaaataac tcgtgccttc ccaaaactgg aaattcgaaa cgaagagcag ggcgccaatc
     5461 gcttccgagc caaggatttg ataggccccg ggctccagca caaaatatcc ggccgcgaga
     5521 agtccaaagc agagacccag aagcccagga atgatgcgtc tggcccgctc gttgtaaaag
     5581 tcccaaaggc cgaaactgcc cctggccagc ctttccgtga tgatggtggt catcagatag
     5641 ccggagatga caaaaaacac atcgactccg acaaagccgc caggaatgaa attgaccttg
     5701 tagtggtaga gcactacggc ggtcaccgcg agcgctctca atgcattgat atcgtagcgg
     5761 aacttcattc gcaccctgtt cgcggccggc ggcgcaccgg ttccttgccg cgcgtcaagc
     5821 tgagatcatt caaggctgac aggacatttc gctatttcgc ctttcagcgg aaacgacggt
     5881 cattgggctt gcgccaggtt agagctttgg agcgacgagc ctatcgtcgg cgcggtgctg
     5941 acccgctttc tgggtcccat aacgtcatcc aaaaattgaa accgtgacgt tcggaccgcg
     6001 gccgagcgat agtgacaatc atccttgaaa tagggtacgc cttcgctgtc caccgtagga
     6061 cagcgctgtt ggtcgcagag gaactcgagc ggatcgacaa atttttcacc tatggatgcg
     6121 gccaaggcca tgagccggct cttcacaggg taggcgcggt cgtcgaacgt agccctatcg
     6181 atatattcga tatccgtggt gtccattcca agaaacctgc gtttggcaag ttcagtcggc
     6241 acattccatc tgcccgaggg cgtggctcca acgatcacaa tctgggttcc acggttcttg
     6301 agttccaaga gccgggagcg caagccggca aatgcggcat ctagaagcgg atagaaggat
     6361 tctggctccc gctccaaacg gcagccgccg cccgtctcga aacacatcca tccatcctgg
     6421 ggattgaagt aaccatacca attcgatacg agcatgatgc gggcaaaatt ccgcgcctcc
     6481 gcgaactgca ttgccttttc gacgaagcca ttgcacttga accggtcgtg aaccgtgcga
     6541 atacccgtta gtggcggaca ggcaggcgat gccaggaagg taaaggagct atcgggatcg
     6601 cggctcacga tttcggcaaa ccggctgtaa atctgcatgg cgaaggaatc gccgatgaca
     6661 agcacgccgc ggtcatttcc tagcccgagc cggcatggtc tcaaattgcc gctcgcgtcg
     6721 gtgccgttgc atgcctccgg atagtcccaa tcatggagcg ccgtctgata ggccaggtac
     6781 tctttgtcac catccggccg gcggttggcg agcccttgat ttatcgtgat cgtgccggca
     6841 aaggccaaag tcagtcccag agcgcttgcg ccccaaatca attcaggcca tctattttcg
     6901 gctagcctcg tctctatgaa ctttctccct agtgacaaca acagcctgcc caccgcgaga
     6961 atggctgcga caatgacgat ttccgcaacg attttgaacg gcgttgttgt tgcaaatcca
     7021 aggtagagcg ccgcaacggc gaccggccag tgccaaaggt atatggagta agaccatact
     7081 ccaatcgtct gtatgagttt gttcttgaag attggcgcat cggcgcggtt tgcagcgatg
     7141 acaaggcatg tacctacgac cggcagcagt ccccagtaat agggccatgg caaattcttg
     7201 tcatagagcg cgatggaaat accgatgaac aatagcccgc tcgcaagaag cgttgcggag
     7261 tatctccggc catcattggc aaattgcagc gcgacaattc cgccggcgat cagctcccac
     7321 gcccgaggcg gcagcagata aaacgcggat ggcggtccat ttgtcgacaa ccaaacgcag
     7381 gacagaaatg acagaagcgc catggaccag aggattggta tgatatggcg ccgcgtttta
     7441 tcgaatttgt acaaacctaa aagtatgatg gggtaggcta agtaaaactg ccattccacc
     7501 gaaaggctcc acgtatggag gagccatttg acgtcgcttt gaggctcgaa atagccggtc
     7561 gcttcccaga aacggaaatt tgaaaagaag agaagagcgg cgatcgacgt cgagccaagg
     7621 taatgatagg ttgccggctc caaaaagagg tagccggcgg caagcagcac gaaacacatg
     7681 ccgagaaggc cggggacgat gcgcttcgcg cggtcatggt aaaattccca aatgctgaac
     7741 ttgcctttgg ccagccttcc catgatgatg ctggtcatga gatagccgga gatagcaaaa
     7801 aaaacgtcga ctccgacaaa tccgcccgga atgaaatcaa ccttgtaatg aaagagaacg
     7861 accgcggtga ccgcgaccgc cctcacggcg ttaatgtcgt aacggaactt catagacccc
     7921 tcgttcaact cgtccttggc ccggttgccg ggctgcgact caagttgcaa tgcgaaacat
     7981 ggcacttgtg ctatccgccg cctcgccgct aggaggcctt gcgacgcgaa tctgagcttg
     8041 cccgcgcagg ccgcgcctcc agcattattg tgccaatttc ccagacttga atattgccta
     8101 gcaaatggct caaacttggc aaaataaggg cgtaggtgaa ccacaacggt ttggcgcaag
     8161 aggctggggc tacgacatct tccggcgcgg cggccggtgt gttagaaggc gaccgaaccg
     8221 cgccggagaa agcccgaacg ggtgccaggt ggggaggtga gtggaacgga catgaaagag
     8281 cggtgcgcct atttcgaata tgaggatctt atcgcctgcg gccgagggga actgtttggc
     8341 ccgggcaatc cccaattgcc gctgccgccg cttttgatgt tcgaccggat ttcggacatt
     8401 tccgagcagg gcggcgccaa tggcaagggc ggagtcgtgg ctgaatattc cgtcaaaccc
     8461 gacctttggt tcttcggctg ccatttcaag ggcgacccgg ttatgcccgg ctgccttggg
     8521 ctcgacgccc tatggcagct cctcggtttt taccttggat ggttagggct aaccgggcgc
     8581 ggccgggcgc tcggcgtcgg cgaggttaag ttttccgatc aagtgttgcc cagcgtcaaa
     8641 cgggtgacct atggcgtcga tctaaagcgc gtgttcaaga ccaaactggt gctgggcatt
     8701 gccgacggct ggctcgatgc ggatggggca cgcatctacg aggccaagga tctgcgggtg
     8761 ggcttgttca aggccacaac gccatccgtg gcctgaggcc gggctgcggg atcgctgtga
     8821 ccttgaacaa tgcggccgcc cggagccgat tcgccgcggg tcgtatcaag caggagtttt
     8881 gaagcatgag acgcgtcgtc gtcactggaa tgggcatcgt ttcctcgatt gggaacaaca
     8941 cccaggaggt gctcggttcc ttgcgcgagg ccaggtccgg aatcgtcaag gcggagaaat
     9001 acgccgagct tggcttccgc agccaggttc atggcgcgcc ggcccttgat ccggaggaaa
     9061 ccatagaccg ccgcgccatg cggttccacg ccggaggcac cggctggaac catgtcgcca
     9121 tggaccaggc aatccttgac gcgggactct cccaagatga aatttctcat gagcgcacgg
     9181 gtattattat gggctctgga ggcccttcga cccgcacgat tgtggaagct gccgacattg
     9241 gccgagccaa gggtccgaag aaaatcgggc ctttcgccgt gccaaaatcc atgtcttcca
     9301 ccgcctctgc cacgcttgcc acttggttca agataaaagg cgtaaattac tccatctcat
     9361 cggcttgcgc gacttccaat cattgcatcg gcaatgctta tgagatgatc cagtatggca
     9421 agcaggacat gatgttcgca ggcgggtgcg aggaacttga ttggaccctt tcggttctat
     9481 ttgacgcgat gggcgcaatg tcctcagcct tcaacgaccg gccagacgcc gcttccagag
     9541 cctatgacat gaaccgggac ggtttcgtca tcgccggcgg agccggcgta ttagtgctgg
     9601 aagaattgga gcatgcccgc gcgcggggcg caaaaatcta tggcgaaatt gccggctatg
     9661 gtctgacgtc cgacggcttc gacatggttg cgccgtctgg ggaaggcgcg gtgcgttgta
     9721 tgcgtatggc acttgaaaac gtcgagacgc cgatcgacta catcaatccg catgcgacct
     9781 cgaccggcat cggcgatctc aaggaaatcg aggcggtgcg ggcggttttc ggttcgggcg
     9841 aaaaatgccc gcccattgct gccacaaaat ccctgactgg ccattcgctt ggggcaaccg
     9901 gcgtgcaaga agcaatctac tcgcttttga tgatgcaaaa tggctttatt tgcgaaagcg
     9961 ccaatatcga cgacctcgat ccagcatttg aggatgttga catcgtgcgc aagcgccgcg
    10021 acggcgtgca attgcgcgcg gtgctttcga attccttcgg cttcggcggc acaaacgcca
    10081 gcatcgttct gaaacatgtc gacgcctgag gcccgtgcca cgctggctca aggaggcttg
    10141 atgcgcggca agcgcggcgt cgtgatgggc gttgcgaatg atcattcgat tgcctggggc
    10201 attgccaagg ccctctatga ccacggagcc gaactggcat tcacctatca aggcgaggcg
    10261 ctcggcaaac gcgtcaagcc acttgccgaa agtctcggag ctccgctcat cctgccctgt
    10321 gacgtcgagg acgcggctgc gatcgatcag ctatttggat ccctcgaaga aatttggggc
    10381 aggctcgatt tcgccgttca ttgcatcgcc tattccgaca aagccgagct caaaggccga
    10441 tattccgaca cgacgcggga aaattttctg cgcaccatgc tgatttctgc tttctccttc
    10501 acggaggtcg cgcgccgcgc tgcggcgctg atgcagccgg gcggcgctct tctgaccctg
    10561 accttcgagg gcgcgtcacg agtcatgccc aactacaatg tcatgggcgt cgccaaggcg
    10621 gcgctcgagg ccagtgtccg ctatctggct gccgatttcg gtcctcatgg cataagggtc
    10681 aatgcgattt cggcgggact tgtgcggacc ttggcagggg cagggatcgc caatgggcgg
    10741 atgatcttca atttccagaa ggcgcacgcc ccgctcaggc gcagtctcga tcttgccgat
    10801 atgggcggtg cggcgcttta tcttctatcg gatctttcaa gcggcgttac cggcgaaatc
    10861 catcatgttg atttgggcta ccatat
//
LOCUS       DATMPU010000044         3905 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_3753299,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000044 DATMPU010000000
VERSION     DATMPU010000044.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 3905)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3905)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3905
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3753299"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(215..661)
                     /locus_tag="VNY06_00985"
     CDS             complement(215..661)
                     /locus_tag="VNY06_00985"
                     /inference="COORDINATES: protein motif:HMM:NF019013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SgcJ/EcaC family oxidoreductase"
                     /protein_id="HWX13448.1"
                     /translation="MVNTVKSLEQVGRNWIAGWNSRDSEAFSRLYAPDGEYVDPSFGI
                     RRKGRDFVRMHHELWWKAIPDFVMKERHIRVADRSVVVEITAEGTFSGGDLGGGKMKA
                     TQKAFRGWTIAVLELNEVGEIVVCTEYYDRSIVPGGEQPPFGELRL"
     gene            829..1041
                     /locus_tag="VNY06_00990"
     CDS             829..1041
                     /locus_tag="VNY06_00990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX13449.1"
                     /translation="MGVFAKVVAKRSFFGAAQEPHGAPGPTQGRIAEMVTPTIASFGT
                     VGMSPLGPFQSWRPDERCPFTGGKQT"
     gene            complement(1121..2554)
                     /locus_tag="VNY06_00995"
     CDS             complement(1121..2554)
                     /locus_tag="VNY06_00995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012111971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HWX13450.1"
                     /translation="MGFAEYSNYDGLGLAELVKKKEIKPAELVEAAMERIERHNPQLN
                     AVVFKAFDEASAMAKTNLSGPFAGVPMLLKDILGFKKGWPNRGGSRFVPAVPSPFDST
                     LVVRFQAAGLIPLGKTNVPEFGALPITEPKLYGPARNPWNLGHSTGGSSGGSAAAVAA
                     GIVPLAHANDGGGSIRIPASCCGLVGLKPTRGRNPLGPVIGDIMGGLVAEHVVSRSVR
                     DTAAVLDATAGPEIGDPYAAPAPPGSYLEATKSEPKRLRIAFTTKAVDGEIDAECKAA
                     SEAAAKLCAKLGHHVEEGRPQLPADDEANFRTNFITIWAAGLAMQVEFLAKRLGKTPA
                     REDFEGLTWGFYQNGKTITAAQYQLCWTRLQASSRQVARWQQPYDAWITPVLGRPPLE
                     IGALNLDETDVMKGLAPILGYVPFTGGQNVTGQPAISLPLAWSKSGLPIGVQFVGRFG
                     EEHLLLQLAAQIERAEPWSNERPPVYG"
     gene            2941..3783
                     /locus_tag="VNY06_01000"
     CDS             2941..3783
                     /locus_tag="VNY06_01000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018648658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1194 domain-containing protein"
                     /protein_id="HWX13451.1"
                     /translation="MRWCISVGAVLVAGAIVGGDVAGVAAPSLKSRFAETGHDVPSVN
                     VELVIAVDISYSMDMDELAIQREGYAQAIVSRDFLQALRAGPDGKVAVTYFEWSMSGD
                     EKIIVPWRVIDGPESADAVAAEIMKSPVRRGSSTSISGAINFAMQLFEENPYRGLRRV
                     VDISGDGPNNDGAPVTGARDAALAKGIIINGLPIMVKEPSYLTTDIEDLDLYYEDCVI
                     GGPGAFIMTINDREKFQEAIRTKLVLEVAGLTLERGIVAAAEEGPRVPCLIGEKKWQE
                     RSGR"
BASE COUNT          770 a         1124 c         1177 g          834 t
ORIGIN      
        1 gccgcgccga cgagctgatt gaatgagcag ccgacttctg ctcacggcac aaagcggaaa
       61 tggcaccatg tctgctctga gtccgctttc gggggtaaag cggaaattag atttgggagc
      121 cgtcaggtca gcttatgacc cgcagcggac tccccgcggg caatccgctg cgatcgtaag
      181 tgtggaccag cgtctttgcg ggtagcataa acatctacag tcgtaattcg ccaaatgggg
      241 gttgttcacc gccaggaacg atgcttcgat cgtagtattc agtacatacc acgatctcac
      301 cgacctcgtt cagttcaagc accgcgatcg tccagccacg gaaagctttt tgggttgcct
      361 tcatcttgcc cccgccgaga tcgccacccg aaaacgtgcc ttcagccgtt atctctacaa
      421 cgacgctacg atccgcgacg cggatgtgcc gctccttcat gacgaaatcg ggtatcgctt
      481 tccaccagag ctcgtggtgc atgcgtacga agtctcgtcc ttttcgacga atgccaaacg
      541 acggatctac gtattcgcca tcaggggcgt acagccgcga aaaggcttcg gaatcgcggg
      601 aattccatcc cgcaatccaa ttgcgcccga cttgttccaa gctcttgaca gtgttcacca
      661 tgacacttct ccttattact acgaagcggc gattgcggcc gcttgggggt ttcgtagtca
      721 tgaaccagag gtattgtcgg caaggcgcta cgataaggcg gccgttcagg atatgatttt
      781 tcacaatgcg tgattaatcg cgggaggcgc gaggattgga tcgagggcat gggcgtcttt
      841 gcgaaggttg tcgccaagcg gagttttttc ggcgccgcgc aggagccgca cggtgcgccg
      901 ggccccaccc aaggccggat tgccgaaatg gtcacgccaa ctatcgcctc ctttggaacg
      961 gtcggcatgt cgcctcttgg cccttttcag tcatggcgtc ctgacgagcg atgtccgttc
     1021 acagggggaa agcagacatg atcttgaagc gccagcactt ccgcttatga cccttagcgg
     1081 aactcgctca attcaaattt gacgtcagct tcttccctcg tcagccgtaa accggcggtc
     1141 gctcgttgct ccacggctca gccctctcga tttgggcggc aagctgcagc agcaagtgct
     1201 cttcgccaaa gcgaccgaca aattgtacgc cgattggcaa gcccgacttc gaccaggcga
     1261 gtggcaggct gatggccggc tggccggtca cgttttgccc gcccgtgaag ggaacatagc
     1321 ccaggattgg cgcaaggccc ttcatgacgt cagtctcgtc gaggttgagc gcgccaattt
     1381 ccagcggcgg tcgcccaagc accggcgtga tccaggcgtc gtaaggctgt tgccatcttg
     1441 cgacttgtcg tgagctagcc tgtaacctcg tccagcaaag ttggtattgc gctgcggtaa
     1501 tggtcttgcc gttttgatag aagccccaag tcaggccttc gaagtcctcg cgcgcgggcg
     1561 tcttacctaa ccttttcgca agaaattcaa cctgcattgc gaggcccgcc gcccagatcg
     1621 tgatgaagtt cgtgcggaag ttggcttcgt cgtctgccgg cagctggggc ctgccttcct
     1681 cgacgtgatg cccgagtttc gcgcaaagct ttgccgcggc ttcgctggcc gccttgcatt
     1741 cggcatcaat ttcgccatcg acggcctttg tcgtgaaggc tatgcggagc ctttttggct
     1801 cgctcttggt ggcttcaaga tatgagcctg gaggtgccgg ggcagcgtag ggatcgccga
     1861 tctctggacc ggcggtggcg tcaagcaccg ccgccgtgtc ccgtacgcta cggctcacga
     1921 cgtgctcggc gacgaggccg cccatgatgt cgccgatcac gggacctagc ggattacgcc
     1981 cgcgcgttgg cttgaggcct acgagcccgc aacacgaggc ggggatgcgg atcgaaccgc
     2041 cgccgtcgtt ggcgtgggcg agcggcacaa tgccggctgc cacggcggca gccgatccgc
     2101 cggaggagcc gccggtcgaa tgaccgagat tccacggatt gcgtgccggg ccgtaaagct
     2161 ttggctcagt gatcggtagg gcgccgaatt ccggcacatt ggttttaccg agaggaatga
     2221 gaccagcggc ctgaaagcgg actaccaacg ttgaatcgaa aggcgaaggt accgcgggca
     2281 cgaagcggga gccgcccctg ttcggccagc ccttcttgaa tccgaggata tccttgagaa
     2341 gcatcggtac gccggcgaaa ggaccggaga ggttcgtctt cgccattgcg ctcgcctcat
     2401 caaaggcctt aaagacaacg gcgttgagtt gcggattgtg ccgctcgatc cgttccatcg
     2461 cggcctcgac gagttcggcg ggcttgatct ctttcttctt cacgagctcg gcaagcccga
     2521 ggccgtcata gttcgaatat tcagcaaaac ccatgctcgc ctcccacgcg tggaaaagac
     2581 agccaaaggc cggcactata taacgttatg ctgtttcgaa attggtgcca atccgcttct
     2641 taagccgacg agattgttga gtagaaggcc ctcaattccg acagtgccgc tcgagtccgt
     2701 tttgtggccc aaagccgaca tgagggaagg cggctctgat gtctgcttcc gcgggcaaag
     2761 cggacattct taagtcctgc agaatgtaag caaatttccc gaaccggact tttccgggct
     2821 tctactcgtt gtcccagatc acgcctcgac tactgcgaac gtgaacgaca attgcgtttt
     2881 tccgcttttc gtggcgcgtt ttgggcgtca ccatccacgc gaacggacgg gagggccgcc
     2941 atgcgctggt gtatctcggt cggggcggtg cttgttgcgg gggcgatagt cggaggtgac
     3001 gttgctggcg ttgccgcgcc gagtctgaaa tctcgattcg ccgaaaccgg gcacgatgta
     3061 ccatccgtca atgtcgagct ggttatcgcc gtcgatatct cctattcgat ggatatggat
     3121 gaacttgcca ttcagcgcga gggctatgca caggccatcg tctcgaggga cttccttcag
     3181 gctctgaggg ccggtccgga cggcaaggtg gccgtgacct atttcgagtg gtcgatgtcg
     3241 ggcgacgaga agatcatcgt tccctggcga gtgatcgacg gcccggaatc ggcggatgcc
     3301 gtggccgccg agatcatgaa gtcaccggtt cgccgaggtt cgagtacctc gatttcgggg
     3361 gcgattaatt tcgcaatgca acttttcgaa gagaatccct atcgcgggtt gcggcgcgtc
     3421 gtcgatattt ccggcgatgg gccgaacaac gacggagccc ccgtaacggg cgcgcgcgac
     3481 gccgccctgg cgaagggtat cattattaac ggcctgccta tcatggtgaa ggagccatcc
     3541 tatttgacga ccgatatcga ggatctcgat ctctattacg aggactgcgt gatcggaggg
     3601 ccgggtgcct tcatcatgac gatcaacgat cgcgaaaaat tccaggaagc gatccggacc
     3661 aagcttgttc tcgaagttgc aggcctcacg ctggagcgcg ggatcgtcgc agccgccgag
     3721 gaggggcccc gggtgccctg tttgatcggc gaaaaaaaat ggcaggagcg cagcgggcgg
     3781 tgagtgtcga gacaatcgtt cagaggcgcc ggacgaacaa acatgtaagc cgcggttacc
     3841 catagcggct tcaacaacag cgcccccgtc gtcaatactg gtatgagttt agcggaatcg
     3901 gccga
//
LOCUS       DATMPU010000045         2530 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_2205531,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000045 DATMPU010000000
VERSION     DATMPU010000045.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 2530)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2530)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2530
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2205531"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..319
                     /locus_tag="VNY06_01005"
     CDS             <1..319
                     /locus_tag="VNY06_01005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003465039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HWX13452.1"
                     /translation="PYIEDGKTIVVSPHKVFGPQTNDLVPQLRKRGIGKIILGGMLAN
                     MCIESHLRELIEQGFEVAVVKDATAGPRHPVWGDGYQAAIINYQFLAHAVLTTNEAVE
                     GMR"
     gene            642..2174
                     /locus_tag="VNY06_01010"
     CDS             642..2174
                     /locus_tag="VNY06_01010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX13453.1"
                     /translation="MDTRAWNSPQSAYAREARCGGSRHAISARKWWLIGMVFAALSLL
                     SAVCSMQPPRFNAEAQVQTGPRLGGVIGLRASAANLNAAGGIGTAIGKARLLASRGLA
                     RRVIKELGIEEYREFDPAANGLGLASRALVFLGIKRDPARVSRDDRVLEAFQERLRVS
                     GPDRQGLLTIAFQAENSELAASAANRLAELYIDMHMDVQKAQPRQAGARIVAFADAPL
                     YPVRVDRLPLLAGMALAVIAFGAFVTIALPRFSLRERRDEAGVEPHPVGCARAIARIK
                     VSERLSPRLTPSLDPPLPITDARAGENPEGGQLIAKVAKRIVSERARVRRCIRILATQ
                     PLAGNAEPFLLLAFARELARGGRAIAICLDSASFLNFGGQAMAPNFKAVPGAGSLLGD
                     LIAGRVLFTEVICRDPASRLHMLPVGQSDDLDLHELENIVDALAETYDFTLIISSPID
                     QVDTAKYVAAKSDFVLLASPVSADGSVIAAEWQLIESGAPEILLIGPETDVGQSLARN
                     AA"
     gene            2332..>2530
                     /locus_tag="VNY06_01015"
     CDS             2332..>2530
                     /locus_tag="VNY06_01015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX13454.1"
                     /translation="MSLPIGRAFALRFVSCQRMDFRRADGYGFRSAAGKASRNLAPSN
                     PDTSNESRNTVGTKSLYRDMHH"
BASE COUNT          521 a          731 c          745 g          533 t
ORIGIN      
        1 gccctatatc gaagatggaa agacgatcgt cgtcagcccg cataaagtgt tcgggccgca
       61 gaccaacgat ctggtcccgc aactgcgtaa acgggggatc ggcaagatca tcctcggcgg
      121 catgttggca aacatgtgca tcgagtccca tctgcgcgag ctaatcgagc agggcttcga
      181 ggttgcagta gtcaaggacg caaccgccgg gccgcggcat ccggtatggg gtgacggcta
      241 tcaagcggcc atcatcaact accaatttct cgcgcatgca gtgttgacga ccaatgaagc
      301 ggtcgaaggc atgcgctgag aacccgcttc cgcatgtcaa gggagctgaa ccatggcgcc
      361 gccaatgaag gtaatctccg tcaatctggg gcggcaagtt gtgccatcct ctcccgcgtt
      421 gaaggaaaag acacggccgc ctacatgccg gcgcgattat gcccgttttc ggaggggaag
      481 gacagcggtt gcgaaatata cttcgcgccg ccaattgtga gtagcggaca agtcccggtt
      541 tttgacgctc acccgtcatt cttatcccga agctccaaaa attaacctcg ctgaaacctt
      601 aacggcgtct tatcgggctg ttcgatcgtt ggagcagcct aatggacacg cgcgcgtgga
      661 attcgccaca gtcggcgtat gcccgagagg caaggtgcgg tggttcgcgc catgccatct
      721 cggctcgcaa gtggtggctg atcggcatgg tgttcgccgc gctcagcctc ctcagtgcgg
      781 tatgcagcat gcagccgccg cgatttaacg ctgaggcgca ggtccaaact ggcccccgtc
      841 tcggcggggt aattggcctg agggcgagcg ccgccaattt gaatgcggcg ggaggaatcg
      901 gcactgcgat tggaaaggcg cgtcttctcg cctcgcgtgg tcttgcccgc agggtcatca
      961 aggagcttgg aatcgaggaa tatcgcgaat tcgacccggc ggcgaatgga cttggcctcg
     1021 cctcccgcgc actcgtattt ctcggcatta agcgcgatcc cgcgcgcgta agccgcgacg
     1081 atcgggttct cgaagccttt caagagcgct tgcgggtgag cgggccagat cgacagggcc
     1141 tcctcaccat cgcttttcag gccgaaaatt cggaactcgc cgcgagcgcc gccaatcgct
     1201 tggcggaact ttacatagac atgcacatgg atgttcaaaa ggcgcagccg cgccaggctg
     1261 gggcccgtat cgttgccttt gccgatgcgc cattatatcc cgttcgcgtg gatagactgc
     1321 cgctcctggc gggcatggcg ttagccgtga tcgcgttcgg tgcatttgtg acaatagcgc
     1381 tgccgcgctt ttccttgcgg gaacgccggg atgaggccgg ggtagagccc cacccagtcg
     1441 gatgtgcgcg cgcaattgcc cgcatcaaag tgagtgaaag actgagcccg cggctaactc
     1501 caagtttaga tccgccgctt ccgatcacgg atgcacgcgc tggcgagaat ccagaaggcg
     1561 gacaactcat cgcaaaggtc gctaaacgca tagtatcaga gcgcgcgcgt gttcgccggt
     1621 gtatccggat tcttgcgacc caacccctgg caggcaatgc tgaacctttc ttgttgctcg
     1681 cttttgccag ggaactcgcg cgtggagggc gcgcaattgc catctgtctg gatagcgcaa
     1741 gctttttgaa ttttggaggt caagccatgg cacccaattt taaggcggtg cccggcgccg
     1801 gctctctgct tggcgacctt attgctggaa gggtcttgtt taccgaagtc atttgccgcg
     1861 acccagcttc gcggcttcat atgctccctg ttggacaaag cgatgatctt gatcttcacg
     1921 aattggaaaa catagtcgat gctctggccg aaacttatga ttttaccctg atcatatcct
     1981 cgcctataga ccaggttgat acggccaaat atgtggccgc gaaatccgat ttcgtgctgc
     2041 tggcaagtcc cgtaagtgcc gatggttccg ttatcgcggc agagtggcaa ttgatcgaaa
     2101 gcggcgcacc cgagattttg ctcattggcc ctgaaacgga tgttgggcaa agcctcgccc
     2161 gcaacgcggc ttgacaaaaa acgccgcaag cgcccaaact caagaattag gtttttccgt
     2221 ccatcgaacg agtggaagcg gcacgatcca gccgataccg agcctgcgag cagagaattt
     2281 ggaggggacc gggcgcctcc cacagcgggc gcgcttttga cgcttgcgcc aatgagcttg
     2341 ccaatcggcc gcgcatttgc cctccgtttc gtgtcctgcc agcgcatgga cttccgccgc
     2401 gctgacggct atggctttcg ctctgccgca ggcaaagcga gcagaaatct ggcgccttct
     2461 aatcctgata catcgaatga atctcgaaac acagtcggta ccaagtcctt gtaccgggat
     2521 atgcaccatg
//
LOCUS       DATMPU010000046         2877 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_1585183,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000046 DATMPU010000000
VERSION     DATMPU010000046.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 2877)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2877)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2877
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1585183"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(221..1213)
                     /locus_tag="VNY06_01020"
     CDS             complement(221..1213)
                     /locus_tag="VNY06_01020"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012563028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HWX13455.1"
                     /translation="MNPASTAKLRSIVLGVGSYLPKRIVSNADLEKSLETSNAWIVQR
                     TGIRQRHVASEDEPTSRLGQLAAEAALAEAGIKATELDLVIVATSTPDYTFPAVATQI
                     QALLGMTNGAAFDVQAVCSGFIFALATADKFLASGSHRRALVIGAETFSRLLDWTDRR
                     TCVLFGDGAGAVVLETQPGYGTAQDRGVLTTQLRSDGRHRDKLYVDGGPSTTRTTGHL
                     RMAGREVFRHAVGMVTDVVSQAFEATGTCAGDLDWFVPHQANRRIIDASAEKLGIAPN
                     KVVITIDLHGNTSAASIPLALAVARNDGRIKRGDLVMIEAMGGGFTWASALIRW"
     gene            complement(1241..2323)
                     /gene="plsX"
                     /locus_tag="VNY06_01025"
     CDS             complement(1241..2323)
                     /gene="plsX"
                     /locus_tag="VNY06_01025"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020175523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HWX13456.1"
                     /translation="MMKPVRIALDAMGGDYGPNVIVPGAARALRLHRDIEFVFFGDEA
                     RIRPLVDMDPELSAVSRVHHTDVSVRMDAKPSQALRAGRRTSSMWLAVEALKKKEADV
                     AVSAGNTGALMAMAKTCLHMMPLIGRPAIAAVWPTLRGESIVLDLGATIGADAQHLVD
                     LAVMGSEMARIVLSIEKPTVGLLNIGVEEIKGNDEVKLASRLLRESIATLDYHGFIEG
                     DDIGKGTVDVVVTEGFTGNIALKTAEGTASQMGQYLREVLSRDLLSKLGYFLARRAFA
                     ALKEKMDPRNVNGGVFLGLNGVVIKSHGGSDAVGTARAVETGYVMARHQLLSKIRNSL
                     PLLHEELSRQPKLETQTAPMAARSEP"
     gene            complement(2664..>2877)
                     /locus_tag="VNY06_01030"
     CDS             complement(2664..>2877)
                     /locus_tag="VNY06_01030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX13457.1"
                     /translation="GSTLSLRMAIGQRWLQGKTKQHLRNTLLVMAAASRNKAPCFDET
                     LSCDRNPPDLGLNPPENEVSAARRMT"
BASE COUNT          628 a          871 c          795 g          583 t
ORIGIN      
        1 caagaattct atcttcgagt caatatcgaa agtagccggg atggatcgaa agtaacctgg
       61 attggcccgg ctatccccaa tttttgcccg ttctgcctgg ccaggcagta aagatagacc
      121 tctgttgacg cggccaatcc gaaccgcgcg acgccagaaa cactgaagca aatcttgaac
      181 gagcgcaaga cgctttgaga cgttggtttg gctatgaagc tcaccagcgg attaacgccg
      241 aagcccaggt gaaaccgccg cccatagctt cgatcatcac caagtcaccg cgcttgatcc
      301 tgccatcatt gcgcgcgact gcaagcgcga gcggaatgga agccgccgaa gtatttccgt
      361 gcaaatcgat cgttatgacg actttgtttg gcgcaatccc aagcttttca gctgacgcat
      421 cgatgatgcg tcggttcgcc tgatgcggca caaaccaatc aagatctccg gcgcaggttc
      481 cggtcgcctc aaaagcttgc gaaacgacat cggtcaccat gccgacggca tggcggaaaa
      541 cttcccgccc cgccattcgc aaatgcccgg tcgttcgcgt cgtggaaggc cctccatcga
      601 catagagttt gtcgcggtga cggccatccg agcgcagttg cgtggtcagc acaccccgat
      661 cttgcgcggt tccgtatccc ggttgcgttt cgagcaccac ggccccggct ccgtcgccga
      721 aaagcacgca cgtccttcgg tccgtccagt cgagaagccg cgagaacgtc tccgcgccga
      781 tcacgagggc acgccggtgc gatcctgagg caaggaattt gtcggcagtc gcaagcgcga
      841 agataaagcc ggagcaaact gcctggacgt caaaggccgc gccatttgtc atcccgagca
      901 gagcttggat ttgggttgcg acagccggaa acgtatagtc aggcgttgag gtcgcgacga
      961 tgacgagatc gagttctgtc gccttaatcc ccgcttcggc gagggcagcc tcggcggcca
     1021 gttggccgag cctggacgtt ggctcatcct cggaagcgac atggcgctgc ctgatccccg
     1081 tgcgctggac gatccatgca ttactcgtct caagcgattt ttcaaggtcg gcattgctca
     1141 cgatgcgttt gggaagatag gagcctactc ctaaaacaat cgagcggagc ttggctgtcg
     1201 atgctgggtt cacgaaatta tccatcggcg cgatgactgc tcagggctcc gagcgcgccg
     1261 ccattggcgc ggtctgcgtt tcgagcttag gttggcggga gagttcctcg tgcaaaagcg
     1321 ggagggaatt gcgaattttg ctcaacagct ggtgtcttgc catcacatag ccggtttcta
     1381 ctgcgcgcgc cgtgccgaca gcgtcggaac cgccgtggct cttaatcacc acaccattga
     1441 ggccgagaaa cacacccccg ttcacattgc gcggatccat cttttccttt aaggccgcaa
     1501 aggcacgccg cgcaagaaaa tagcctaact tcgaaagcaa atcccggctc aggacttcgc
     1561 gaaggtattg ccccatctga ctggccgtcc cttccgccgt cttcaaagct atattgcctg
     1621 tgaaaccttc ggtaacgaca acatcgacgg ttcccttgcc gatatcgtca ccttcgatga
     1681 aaccgtgata gtcgagagtg gcgatactct cgcgcagcaa ccgggaagcc agcttgacct
     1741 cgtcgttgcc cttgatttcc tcaacaccaa tattcagcaa acccaccgtc ggcttctcaa
     1801 tcgacagaac gatgcgcgcc atctcggagc ccatgacggc aaggtcgaca aggtgctgtg
     1861 catcggcgcc gattgtggcc ccgagatcta gaacgatgga ttcgccgcga agcgtcggcc
     1921 aaaccgcggc aattgccggc cggccgatca atggcatcat atgcaggcac gtcttcgcca
     1981 ttgccatcaa cgcgcccgtg ttgccagccg aaaccgcaac atcggcctcc ttttttttca
     2041 gcgcctcaac ggcaagccac atcgacgatg tccgtcgccc ggcgcgcaac gcttgactgg
     2101 gctttgcgtc catccgaacc gaaacatcgg tatgatgcac cctggaaacc gctgaaagtt
     2161 ccgggtccat atcgacaagc ggccgaattc ttgcttcatc accaaaaaac acaaactcaa
     2221 tatcgcgatg cagccgcagc gcgcgggcgg cgcccgggac gatgacgttc ggaccataat
     2281 caccgcccat ggcatcaagc gcaatgcgta ctggcttcat catggactgc gcgactccgt
     2341 gccggcggca ccactttgca tggcacctgg tcctggtttc ttagcgacga aagcattgga
     2401 aaaatgaact cagtgcggcg gcaatggacc ccccggatgg cgctccctgc cgccatgcag
     2461 aggcgtgagt atcgcgcatt gctcttccgc cgaatggacg gcgaccatgg gtaaacaacg
     2521 gaagatcgcc atcattcaag ttttttttgg cagaaacacg aaccaggact tctcggaatt
     2581 tttgcaacgg tgcggctcgc ggtgtcgcag ggaggccgca atctgcgtcc agacaaactg
     2641 acgcacgaga gtcaaggcaa agctcacgtc atcctgcgcg ccgcggagac ctcgttctcg
     2701 ggagggttaa gtccaagatc cggagggttt cggtcgcaag acaaagtctc gtcaaaacag
     2761 ggagctttgt tcctgcttgc ggcggccatg accaggagcg tgtttcggag gtgctgcttt
     2821 gttttccctt gaagccaccg ctgcccgatg gccatccgca aactcaatgt cgagcca
//
LOCUS       DATMPU010000047         5959 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_2823780,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000047 DATMPU010000000
VERSION     DATMPU010000047.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 5959)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5959)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5959
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2823780"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..198
                     /locus_tag="VNY06_01035"
     CDS             <1..198
                     /locus_tag="VNY06_01035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014893112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HWX13458.1"
                     /translation="VKWFNAQKGFGFIEPESGGADVFVHISAVERAGLRGLAEGQKVS
                     YEIVVDSRNRKSSADQLQIAA"
     gene            308..574
                     /locus_tag="VNY06_01040"
     CDS             308..574
                     /locus_tag="VNY06_01040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003612518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX13459.1"
                     /translation="MSANTRELSRHQRLRSKAVPTHKFRVGAYVLHRIGMRSEKASFR
                     VTRLLPDGGAGLQYRIKGERDGIERVVTESFIEPAGENLSFSPP"
     gene            646..915
                     /locus_tag="VNY06_01045"
     CDS             646..915
                     /locus_tag="VNY06_01045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016921306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX13460.1"
                     /translation="MSTFDYGLEAGLFSFKGPNKGPKFLQKALGYRRFARAADAIRFV
                     MEDIPSSMLQGCSLEVDEATYVGAAIRDLYESSDFPLPRQVKSSK"
     gene            1281..3920
                     /gene="mutS"
                     /locus_tag="VNY06_01050"
     CDS             1281..3920
                     /gene="mutS"
                     /locus_tag="VNY06_01050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011995714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HWX13461.1"
                     /translation="MMAQYHAIKAQNPGSLLFYRMGDFYELFFEDAEIAAKALGIVLT
                     RRGKHEGQDIPMCGVPVERAEDYLHRLISQGYRVAVCEQVEDPAEAKKRGAKSVVRRE
                     VVRLVSPGTITEELLLNPLRANCLLAIARGSAPDRPYGLACVDISTGTFLVVDAAEDS
                     LEAEISRLDPAEIVAPEAVCTLPCLARLALETKLSLTPLGNEAGVGPSAERRITDFFG
                     LATLDGLGNLSSAEIAAAAVALLYIERTQISARPALNLPMRIARDALMGIDAATRANL
                     ELGWTLAGRREGSLLATIDKTVTAPGSRLLAERLAAPLTDPQFISARLDAVAFFVDEP
                     GLRKTVRACLKSAPDMTRALSRLAVGRGGPRDLAALRDGLAAAHALADVLAKRPVIPE
                     ELADATEAVRSVDPTVIGSLADALAEALPLNRRDGGFVRSGHDRALDALRALRDESRS
                     VLAALQARYADQLDTKQLKLKHNHFLGYFLEVPQAQGERLKKPPHDTTFVHRQTMAGA
                     MRFSTKELAELETKIASAAEEALAREQAIFDGLASFLLANEAALKRAGGALAAFDVAA
                     ALAELAEGLGWTRPQVDRSLSFAIENGRHPVVEQALRLQGQPFAGNDCELSGAPGIGG
                     KIAIVTGPNMAGKSTYLRQNALIAILAQMGSFVPAKRALIGVFDKLFSRVGAADDLAR
                     GRSTFMVEMIETAAILNQATERSLVILDEIGRGTATFDGLSIAWAVIEHLHERNRARA
                     LFATHFHELTKLSEKLVRLVNLTVRVTDWHGDVIFLHEIIYGAADRSYGIQVAKLAGL
                     PAAVVDRARTLLAEFEAAERLAPIDKQLSELPLFAARAGSEFQPRIGEIEKALDEIEP
                     DDLSPREALEVLYRLKGIHAREG"
     gene            4064..4546
                     /locus_tag="VNY06_01055"
     CDS             4064..4546
                     /locus_tag="VNY06_01055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012591325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HWX13462.1"
                     /translation="MADAAVKSRLEQQVCFAIYSAMHAVNKAYMPLLEKIALTYPQYL
                     AMLVLWESDDVTVKQLGERLFLDSGTLTPLLKRLEGMGLVDRARDPRDERKVRLSLTD
                     KGKELRREALSIPGQIAAAMGRPADDLKAVRKELRRIRNSLLQGRAQKFLESSDAGFD
                     "
     gene            complement(4555..5055)
                     /gene="ruvX"
                     /locus_tag="VNY06_01060"
     CDS             complement(4555..5055)
                     /gene="ruvX"
                     /locus_tag="VNY06_01060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020175304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HWX13463.1"
                     /translation="MAAEVLDLEALASRLHPRQRLMGADLGTKTIGLALSDIERRIAT
                     PLITIRRTKFSKDAAWLISLIDQFNAAGLVIGLPLNLDGSEGPSAQAARAFGRNFAPL
                     SGRPCLFWDERLSTEAVTRSLIAQDASRAQRAEVVDRMAAAYILQGALDRLRALASAM
                     ERDNKP"
     gene            5136..5834
                     /locus_tag="VNY06_01065"
     CDS             5136..5834
                     /locus_tag="VNY06_01065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010909577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HWX13464.1"
                     /translation="MKLIWFGHSAFRIELPAAVILIDPFLTGNPKFSGSAEKAADGAT
                     HIVLTHGHDDHVGDAAKIAKNTGAQLISNFEICMYLHAQGAQNINPGNTGGTIDCGSF
                     SVSFTQALHSSSTIVNGQSVYLGSPNGMVIKPKDAPSIYHMGDTDLFSDMALIAEIYA
                     PEIGLVPVGDRFTMSGKTAALAVKRYFHFDTAIPCHYGTFDVLAPDASAFVAAMQGHP
                     TKVMVPRIGEVLGF"
BASE COUNT         1211 a         1713 c         1765 g         1270 t
ORIGIN      
        1 gtaaaatggt tcaacgctca gaaaggtttc ggattcatag agcccgagtc cgggggggcc
       61 gacgtcttcg ttcatatcag cgccgtcgaa cgtgcgggcc tgcgtggtct cgccgagggc
      121 cagaaggtgt catacgagat cgtcgtcgac tcacgcaacc gaaagtcttc ggcggatcag
      181 ctgcagatag cagcctgagc cggccctgat ctcgatcgcg ctgaccgcgg cgagcctggt
      241 tgggcggaac cgtatcgatg gtcgcgcgag tcgagttcgc cagatctcca tcaggaaagg
      301 gccgtatgtg agtgcgaata ccagggagct atcccgccat cagcgacttc gtagcaaagc
      361 ggtgccgacg cacaagtttc gcgtcggcgc ttatgttctc cacaggatcg gcatgcgatc
      421 ggaaaaagcc tcctttcggg tcacgcgcct gctaccggat ggcggtgcgg gacttcaata
      481 tcggatcaag ggagagcggg acggcatcga acgagtggtt acggagtcgt ttattgagcc
      541 tgccggcgag aacctttcgt tctcgcctcc ctgacagcgc aatcagcggc taaggtgttt
      601 tctggcagtg gccgcaacaa ccgagccgag cagaagggat gaacgatgag cacgttcgat
      661 tacggcctgg aagcgggatt gttttcgttc aagggtccga ataaggggcc aaaatttctg
      721 cagaaggctc tcggatatag acgatttgcc cgggccgcgg acgcaattcg ctttgtgatg
      781 gaggatattc cctccagcat gctgcagggc tgctctctcg aggttgacga agcgacctat
      841 gtgggcgcgg cgatccgcga tttgtatgaa agctccgatt tccctctgcc acgacaggtc
      901 aagtcttcga aataatctcg atagctcccc gccggaagat tcgacgccga gtctgcaaat
      961 gctagcgcgc tcaggatcga cttgcactga ggctgctttt cacaatgaca tgcgttggcg
     1021 aaacgctgtc gctactcgat tcgaatgtca tcggaaaatt aagcgatcta acaagggtga
     1081 atgacaagcc attgtttggg tctttataag attttgctct gcaatcagac aaaaatgttt
     1141 cgggtttgcg catatgggcc agtgcatcgc gccgaggcct ttgctaggct cggctcatgc
     1201 ccaaaagcgc tgctttccca acaacgccgt ctcccggtcc cgtgccagca aataacccgg
     1261 ccgagccaag actatcgccg atgatggcgc aatatcacgc gatcaaggcg caaaatcccg
     1321 gctcgctgct tttctaccgg atgggggatt tttacgaatt gtttttcgag gatgcggaga
     1381 tcgccgccaa agcgcttggc attgttttga caaggcgtgg caagcatgag gggcaggata
     1441 tcccgatgtg cggcgtgccg gtcgagcgcg ccgaggatta tctgcatcgg ctgatcagcc
     1501 agggctatcg cgtggctgtg tgcgagcagg tcgaagatcc agccgaggcc aaaaagcgcg
     1561 gtgccaaatc cgtcgtccgg cgcgaggtgg tgcggctggt gagtcccggc accattaccg
     1621 aggagctttt gctcaatccc cttcgcgcca actgcctgct tgcgatcgcg cgagggagtg
     1681 cgccggatag gccctatggg ctcgcttgcg tcgacatatc aaccggcaca tttttggtcg
     1741 ttgacgccgc cgaggattcg cttgaggctg agatttcgcg cctcgatccg gccgagattg
     1801 tcgcgcccga agccgtttgc acattgccat gcctcgcgcg tctcgcgctc gagaccaaac
     1861 tttcgctgac cccactggga aatgaagcgg gcgttgggcc aagtgccgag cgccggataa
     1921 cggatttttt tgggctcgcg accctcgatg gtttgggcaa tctttcaagt gccgaaatcg
     1981 ccgctgcggc cgttgccctt ctctatatcg agcgcacgca aatttccgcg cggcctgcct
     2041 taaacttgcc aatgcggatt gcgcgcgatg cgttgatggg catcgatgcg gcgacgcgcg
     2101 caaatctcga acttggctgg acattggcgg gcaggcggga gggctccctg ctcgcaacca
     2161 tcgacaagac cgtcaccgcg ccgggcagcc ggctgcttgc cgagcgcttg gccgctcctc
     2221 tgaccgatcc gcaattcatc tcagcgagac tcgatgcggt cgcctttttt gtcgatgagc
     2281 caggcctgcg caagaccgtg cgcgcctgcc tcaaatctgc accggacatg acccgcgccc
     2341 tgtcccgcct tgccgtcggt cgcggcggac cgcgcgatct tgcggcgctc cgcgacgggc
     2401 ttgccgcggc tcatgcgctg gctgacgtcc tggccaagag acctgttatt ccggaggagc
     2461 tcgccgacgc gaccgaggcc gtgaggagcg tggacccgac tgtgatcggt agcctagcgg
     2521 acgcgcttgc ggaagcgctg ccgctcaacc gccgcgatgg cggctttgtc cgctcgggcc
     2581 acgatagggc cctcgacgcc cttcgcgcct tgcgggatga aagccgaagt gtgcttgccg
     2641 cgcttcaggc gcgctatgca gatcaattgg acacaaagca attaaagctc aagcacaatc
     2701 attttcttgg ttattttcta gaggttccgc aggctcaggg ggagcggctc aaaaagccac
     2761 cgcatgatac gacatttgtg catcgccaga caatggcggg cgcaatgcgt ttttcgacca
     2821 aagaacttgc cgagctcgaa accaagattg cttctgctgc cgaggaagcg ctcgcccgcg
     2881 aacaggcgat cttcgatgga ttggcgtcat tcctccttgc caatgaagcc gcgctcaagc
     2941 gagcaggtgg tgcgcttgcg gcatttgatg tcgcggcggc cctggccgag ctggccgagg
     3001 gactgggttg gacgcggccg caggtcgatc gctcgctttc cttcgcgatc gagaatgggc
     3061 gccatcctgt cgttgaacaa gccttgcgcc tccaaggtca gccatttgcc gggaatgact
     3121 gcgaactttc cggcgcgccc ggcatcggcg gcaaaatcgc gatcgtgacc ggcccgaata
     3181 tggcgggcaa gtccacctat ctgcggcaaa atgctttgat cgccatcctc gcgcagatgg
     3241 gctccttcgt gcccgcgaaa cgcgccctta taggcgtatt tgacaagctt ttctcccggg
     3301 ttggcgcggc agacgatctc gcacgcggcc gttcgacctt catggtcgaa atgatagaga
     3361 ccgcagcgat cctcaatcag gcgaccgagc gctcactcgt cattcttgac gagattggtc
     3421 gcggcaccgc gacattcgac gggctttcca tcgcctgggc ggtgatcgag catttgcacg
     3481 aaagaaatcg cgcccgcgct ctcttcgcaa cgcattttca tgagctgacg aagctttccg
     3541 aaaagctcgt ccggctcgtc aatttgaccg tgcgggttac ggattggcac ggtgacgtaa
     3601 ttttcttgca cgagattata tatggagccg ccgatcgttc ctatggcatc caggtcgcga
     3661 agcttgcggg tctccctgcc gcagttgttg accgcgctcg aacgcttctt gccgaattcg
     3721 aggcggcgga aaggctggct ccaatcgaca aacagctctc tgaattgccc ttatttgcgg
     3781 cgcgggcagg ctcagaattc cagccgcgaa tcggcgagat tgagaaagca ttggacgaga
     3841 tcgagccgga cgacttatct ccgcgtgagg cgctggaagt tctttaccgg ctcaagggaa
     3901 tccatgctcg ggagggttaa aaactgttcg aatgagattt atcgcccaac agttaccaag
     3961 ggccggaggc ccaatcgtat aaaaatatac ttagcttagg gatgcatgaa actcgcaaat
     4021 gatatacact acaattaacc acaattgaat taagggctgc atcatggcgg acgccgccgt
     4081 aaagtcgagg ctcgaacagc aggtctgttt cgcaatttat tcggcaatgc acgccgtcaa
     4141 taaggcctat atgccgctgc tcgaaaaaat tgctctcact tatccacaat atcttgccat
     4201 gctggtgctc tgggaatccg atgatgtcac ggtcaagcag cttggcgagc gcctctttct
     4261 cgattcaggc acgctcacgc cgcttttgaa acggctcgag ggaatgggcc tcgtggatcg
     4321 cgcgagagat ccgagggacg aacggaaggt gcgcctgtcg ctcaccgaca agggcaagga
     4381 gcttcggcgc gaggcgctgt ccatcccggg ccagatcgcg gccgcaatgg gccggccggc
     4441 ggatgatctt aaagccgttc gcaaagaact ccggcgaata agaaattctc tgttgcaggg
     4501 ccgcgctcag aaattcttgg aaagtagtga cgccggcttc gattagaact cagcctatgg
     4561 cttgttgtct ctttccatgg cggacgcgag cgcgcgcagc cggtcgagcg ccccttgtaa
     4621 gatgtaagcg gcagccatcc gatcaacgac ttcggcgcgt tgggcgcggg atgcatcttg
     4681 cgcaatcaaa gagcgcgtaa cagcctcggt cgaaagccgt tcatcccaga ataagcaggg
     4741 gcgcccggaa agcggcgcga aattgcggcc aaaagcgcgg gctgcctggg cgctcggccc
     4801 ctctgagcca tccaaattga gcggtaggcc gattacaagc ccggccgcat taaattgatc
     4861 gatgagcgaa atgagccacg cggcatcctt gctgaacttt gtgcgcctta tcgtgatcag
     4921 cggtgtcgcg atgcgccgct ctatatcgga aagagcaagg ccgatggttt tggtgccaag
     4981 gtcagcgccc atcagccgct ggcgcggatg aaggcgcgaa gccaaagctt cgaggtccaa
     5041 gacttccgcc gccatcagcc gcacttggtg tattggtacg agcccgctat atcctgcatc
     5101 ttgccctgct ccttcaagtc ctttcggggt cccgcatgaa gctcatctgg tttggtcatt
     5161 ccgctttccg cattgagttg cccgctgccg tcattctcat cgatccgttc ttgaccggca
     5221 acccgaaatt ttcgggcagc gccgaaaagg cggccgacgg ggcgacccac attgtgctca
     5281 cccacggcca tgacgatcat gtcggcgatg ctgccaaaat tgctaagaat accggcgctc
     5341 agcttatctc gaatttcgaa atatgcatgt atttgcatgc gcagggcgcg caaaatatca
     5401 acccgggcaa cactggcggt acgatcgatt gcggttcttt cagcgtgagt ttcactcagg
     5461 cgctgcactc ctcttcgaca atcgtcaacg gtcaatccgt ttatcttggc agtcccaacg
     5521 gcatggtcat caagcccaag gatgcgccga gcatttacca catgggcgac accgatctct
     5581 tttcggacat ggctctgatc gcggaaattt atgcgccgga aattggcctc gtgccggtcg
     5641 gcgaccgctt cacgatgagc gggaagaccg ccgcgctcgc ggtcaagcgg tatttccatt
     5701 tcgacaccgc catcccatgt cactatggta ccttcgatgt cctcgctccg gacgcgagtg
     5761 cctttgtcgc ggcgatgcaa ggccatccga caaaggtcat ggtgccgcgc atcggcgaag
     5821 ttttggggtt ttgacggcca cgcttgcctt tgcaggcgta tgccaaaatg aaatggcgga
     5881 gatgaacggc aatgagcgat ttttttgggc tcgacgatca gataaccggc gacatccgcc
     5941 tcaatggcat cggctggat
//
LOCUS       DATMPU010000048         2458 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_658677,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000048 DATMPU010000000
VERSION     DATMPU010000048.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 2458)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2458)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2458
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_658677"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1357
                     /locus_tag="VNY06_01070"
     CDS             <1..1357
                     /locus_tag="VNY06_01070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020177338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HWX13465.1"
                     /translation="PVADNPLGEPAFRAGPDTPSVNGLTGGLLTANPNLVNPFRLDRG
                     QAATCDQDHDYTAEQQAFDAGLMDKFPQFAGVGAPSCPAYGKGKNLVMGYFDGNTVTA
                     LWNYAQTFAMSDNSFGTTFGPSTPGAINLISGQTNGASPANQSGDTVQGTIIGDPQPT
                     GDTCTTRDNVKMVTGKNIGDLLNAKGISWGFFQGGFDLTVTNPDGSTGCNRTHTSTVT
                     GTKKVDYIPHHQPFQYYASTANLNHTRPSSVAAIGTTDGANHQYDIHDFFDALANGNF
                     PAVSFLKAPGYRDGHAGYSSPLDEQAFVVVTINTLQRDPEWSSTAVIIAWDDSDGWYD
                     HQLGPIVSQSSTTSDALTGPNACGQAPSGAAQGRCGYGPRLPLLVVSPFAKRNFVDNT
                     VTDQSSILRFIEDNWGLGRTGKGSFDAIAGSLQNMFDFSARPRQDRLCLDPFTGEPAG
                     C"
     gene            1688..>2458
                     /gene="hpnH"
                     /locus_tag="VNY06_01075"
     CDS             1688..>2458
                     /gene="hpnH"
                     /locus_tag="VNY06_01075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017486661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosyl-hopene transferase HpnH"
                     /protein_id="HWX13466.1"
                     /translation="MGIPVLQMAQIGAYVVRQRLAGRKRFPLVLMLEPLFRCNLACAG
                     CGKIDYPDKILNQRLPLADCLAAIDECGAPVVVVAGGEPLLHKDLPQIVKGAIARGKF
                     VTVCTNALLLAKNLDRYTPDRYFNWSIHLDGDAEMHDRSVCQAGVYERALEALKLAKE
                     RGFRVTVNCTLFNNADSVRVAAFFDSVAALGIEGITVSPGYAYERAPDQKHFLNREKT
                     KTLFRQIFALGKGGKAWPFFQSKLFLDFLAGNRTYRCTP"
BASE COUNT          514 a          756 c          677 g          511 t
ORIGIN      
        1 tcctgttgcg gacaatccgc tgggtgagcc cgcattcagg gcgggacccg atacgccgtc
       61 ggtgaacggg ctcaccggtg ggctgctgac cgccaacccc aaccttgtca acccattcag
      121 gctcgatcgc ggccaggcgg ccacttgcga tcaggatcac gattatactg ccgagcagca
      181 ggccttcgac gcgggattga tggacaaatt tccgcagttt gccggcgtgg gcgccccaag
      241 ctgtccggcc tatggcaagg gcaagaattt ggtcatgggc tatttcgacg gaaacacagt
      301 cactgcgctg tggaattatg cgcagacgtt cgcgatgagc gataactcct tcggcacgac
      361 ttttgggccg tccacaccgg gcgctatcaa cctgatctct ggccagacca atggcgcaag
      421 cccggccaat caatctgggg ataccgttca aggaacgatt atcggcgatc cacagccaac
      481 gggcgacacc tgtacgacac gcgacaacgt aaagatggtc accggaaaaa acatcggcga
      541 tctactgaat gccaaaggca tatcctgggg cttctttcaa ggcggatttg atctcacggt
      601 caccaatccg gatggcagca ccggatgcaa ccggactcac acttcgactg taaccggtac
      661 caaaaaggtg gattacatcc cccatcacca gccattccaa tattacgcgt cgaccgccaa
      721 tctgaaccac acccgcccaa gctcggtcgc ggcgattggc acaaccgatg gggcgaacca
      781 tcaatacgat attcatgatt tcttcgatgc cttagccaac ggaaatttcc cggcagtctc
      841 ctttctcaaa gcaccaggct atagggatgg ccacgctggc tactcttccc cgctcgacga
      901 gcaagccttt gtcgtcgtga ccatcaacac cctgcaaagg gatccggaat ggagctcgac
      961 cgcggtcatc atcgcttggg atgactccga cggctggtac gatcatcaat tgggtccgat
     1021 tgtgagtcag tcgagcacga cgtccgacgc actaactggc cccaacgcct gcggccaagc
     1081 accgagtgga gccgcgcagg gccgctgcgg atatggtccc cgtctgccgc ttctggtggt
     1141 ttcgcccttc gccaagcgca acttcgtcga taacaccgtg accgaccaga gctccattct
     1201 gcgtttcatt gaggataact ggggcctcgg ccgcaccgga aaaggctcgt ttgacgcaat
     1261 cgctggttcg ctgcaaaata tgttcgactt cagcgccagg ccgaggcaag ataggctttg
     1321 cctcgatcct ttcaccggcg agcccgcggg ttgttaatga tttaacttgc accggccgga
     1381 gcgccattac agacgctcca gcctataatc tgcgccaatc tttagagagc cgccttttgg
     1441 gcgccagcgg gtttaagccg cagcgttaga aattgcagcg atagaaatta cagcaggtcg
     1501 gcacatcgag catgatcgca tccttggccg ccagtatacc gcacgcgctg atcggctttc
     1561 ggcccggccg aattgacacc cttgcctgta atcgcggaaa aggtagaatt caaacttccg
     1621 gccggactca acgattgatc ctcgcgcggc ccgaccgtgg gtggcggcaa atttttagga
     1681 gcacgctttg ggcataccag ttctgcaaat ggctcagatc ggcgcctatg tggtgcgcca
     1741 gcgcctggcc gggcgcaagc gctttccgct cgtcctgatg ctcgaacctc tgttccgctg
     1801 caatctcgct tgcgcgggct gtggcaagat cgattatcca gacaagattc tcaaccagcg
     1861 tctgccactc gccgactgcc tcgcggcgat cgacgaatgc ggcgcgcctg tcgtggtggt
     1921 cgccggcggc gagccgctgc tgcacaagga tcttccccag atcgtgaagg gcgcgatcgc
     1981 cagaggcaaa tttgtgaccg tgtgcaccaa cgcgcttctg ctcgcgaaaa atctcgatcg
     2041 ctatacgcct gaccgctact tcaattggtc gattcatctc gatggcgacg cggaaatgca
     2101 tgaccgttcg gtctgtcagg ccggtgtcta cgagcgtgcc ctcgaggcct tgaagctcgc
     2161 caaggagcgc ggttttcgcg ttaccgtcaa ttgcacgctg ttcaacaatg ccgattccgt
     2221 ccgagtcgcg gcgtttttcg atagcgtggc ggcgcttggc atcgagggca tcacggtgtc
     2281 gcctggctac gcctatgagc gcgcccccga tcaaaagcat ttcctcaatc gcgaaaaaac
     2341 caagactcta ttcaggcaaa tttttgcgct gggcaagggc ggcaaggcgt ggcccttctt
     2401 ccagtcgaag cttttcctcg actttctcgc cggcaatcga acctatcgct gcacgccc
//
LOCUS       DATMPU010000049         3808 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_349027,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000049 DATMPU010000000
VERSION     DATMPU010000049.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 3808)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3808)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3808
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_349027"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..348
                     /locus_tag="VNY06_01080"
     CDS             <1..348
                     /locus_tag="VNY06_01080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012606126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HWX13467.1"
                     /translation="LPSCNTEAMALHLEEISLAVAPGAHALVLLDQAGWHVSTKLPIP
                     DNITLLPLPPKSPELNPVENIWQFMRDNWLSNRIFKSYEDVLDHCCFAWNKLIDMPWK
                     TISIGTRDWAYRS"
     gene            473..1027
                     /gene="cobU"
                     /locus_tag="VNY06_01085"
     CDS             473..1027
                     /gene="cobU"
                     /locus_tag="VNY06_01085"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012386500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0043752 - adenosylcobinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HWX13468.1"
                     /translation="MPGAGIRAKSLLVLGGARSGKSRYAQRLAESSRLQPVLIATAEA
                     RDAEMALRIERHAAERSAQWTVVEEPVALAEALWREARKERIVVVDCATLWLSKILLE
                     GDNIAAATKDLAQSVAKLSGPVIFVSNEVGGGVVPENGLARAFRDAQGLLNQALAEAC
                     EAVVLVSAGIAVCLKPSPAPVFRF"
     gene            1120..2028
                     /gene="cysD"
                     /locus_tag="VNY06_01090"
     CDS             1120..2028
                     /gene="cysD"
                     /locus_tag="VNY06_01090"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012383088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA];
                     GO_process: GO:0019419 - sulfate reduction [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HWX13469.1"
                     /translation="MKILLSPHLRRLESESIFIMREVAAEFVRPVMLYSIGKDSSVML
                     HLAQKAFYPSKPPFPLLHVDTTWKFREMIAFRDEAAKRAGMELQVYINKEGLKRGVSP
                     IASGSSVHTRVMKTEALRQALDLGHFDAAFGGARRDEEKSRAKERIFSHRSAAHAWDP
                     RNQRPELWQLFNTKIKPGESMRVFPLSNWTELDVWDYILAQDIPVVPLYFAKERPVVK
                     RDGALIMVDDERLPLLPGETPHMKRIRFRTLGCYPLTGAIRSDAGTLPEIIAEMRASR
                     TSERQGRLIDHDESGSMEKKKREGYF"
     gene            2028..>3808
                     /gene="cysN"
                     /locus_tag="VNY06_01095"
     CDS             2028..>3808
                     /gene="cysN"
                     /locus_tag="VNY06_01095"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020174711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysN"
                     /protein_id="HWX13470.1"
                     /translation="MLYATQEILRLQSDTAALPTLRLLTCGSVDDGKSTLIGRLLYEQ
                     SLIPDDQLAALECDSKKHGTDGDNIDFALLVDGLEAEREQGITIDVAYRYVSTPKRSF
                     VIADTPGHEQYTRNMATGASGADLAILLVDARKGILTQTCRHAAIVSLTGIKNVVLAV
                     NKIDLIGFDQACFDKISADFIGFATALKFKSVTPIPISARYGDNISKPSANTPWYRGP
                     TLLDYLETIGIAEDRAKEPFRFLVQWVNRPHLDFRGFSGAVASGTIRQGDELTVASSG
                     RMSRVARIATADGDLESASAGDSVTLTLTDELDIARGDILCDPKNRPEVAEQFAAHLF
                     WMSDDKLLPGRSYLLKTGARTVPVSVTELKYRLDVNSLSELAAKTLRLNEVGFCNLAA
                     TTPIAFDPYKKNQATGAFILIDRLTNATAAAGMIAFGLRRATNIHLQGLSVSKLDRCQ
                     IKHHQPAVLWFTGLSGSGKSTIANLVESRLCANGVHTILLDGDNVRHGLNNDLGFTEV
                     DRVENIRRIGEVAKLMTQAGLVVLCSFISPFRAERRMVRELLDRQEFIEIYVATPLED
                     CIKRDPKGLYKKAMAGEIKNFTGVDQA"
BASE COUNT          818 a         1099 c         1056 g          835 t
ORIGIN      
        1 cttccctcgt gcaacaccga ggccatggct ctccatctgg aagaaatctc gcttgcggtc
       61 gcgccgggcg ctcacgcgct cgttcttctc gatcaagccg ggtggcatgt ctcgacgaag
      121 cttcctatcc cagacaacat caccctcttg ccgttgccac caaagtcacc cgagctcaat
      181 cccgttgaaa atatctggca gttcatgcgc gacaactggc tctcaaaccg catcttcaaa
      241 tcctacgaag acgttctcga ccattgctgc ttcgcctgga acaagctcat cgacatgccc
      301 tggaaaacca tctctatcgg aacccgagac tgggcttatc ggtcatgatc aacgagactt
      361 ggtataagcc ctccagccga gggtatttgc aggcaaattg accctgggac aaacgcggga
      421 gcgatccacc atttgacagc gaaaattcgg ttccgctagg gtagcggtca aaatgccagg
      481 cgccggaatt cgagcaaaga gccttcttgt ccttggcggc gcgcgttccg gcaagagccg
      541 ctacgcgcaa aggctagcgg aatcgtctcg ccttcagccg gtcctgatcg caacagcgga
      601 agcgcgtgac gccgaaatgg ctttgcggat cgaacgccac gccgctgagc ggagcgcgca
      661 atggaccgtc gtcgaagagc cagtcgcgct cgccgaggcg ctttggcggg aagcccgaaa
      721 ggagcggatc gtggtggtcg attgcgcaac gctttggctg agcaaaatcc tgctggaggg
      781 tgacaatatc gccgccgcga caaaagactt ggctcagagc gtcgccaaac tctccggtcc
      841 cgtgatattt gtatcgaacg aagtgggagg tggcgttgtg ccagaaaatg gtctcgcgcg
      901 ggctttccgg gacgctcagg gtctcttaaa ccaagctctg gcggaggctt gcgaggctgt
      961 cgttttggtg agtgccggga ttgctgtttg cctcaaacct tcgccggcac cagtatttcg
     1021 cttctagttc tgcttgtacc tgcgacattg aaatggcgaa agcagtggca taataacaca
     1081 tcgaattacg ttaaattttg catatgacag gtgcagtttg tgaaaattct tctgagcccc
     1141 catttgcgcc ggctggagtc ggaatccatc tttatcatgc gggaggtggc ggcagagttt
     1201 gtccgccccg tgatgctcta ctcgatcggc aaagactcaa gcgtgatgtt gcatcttgcg
     1261 cagaaggcct tctatccatc aaaaccgccc tttccgctgc ttcacgtcga taccacttgg
     1321 aaattccggg aaatgatcgc cttccgcgat gaagcagcga aaagggcagg gatggaattg
     1381 caggtttaca tcaacaagga gggcctcaag cgcggcgttt cgccgattgc atcggggtcg
     1441 tccgtgcata cgcgagtgat gaaaaccgag gcgctgcgtc aggcactcga tcttgggcat
     1501 tttgatgcgg cctttggcgg tgccaggcgc gatgaggaaa agagccgggc caaggaaagg
     1561 attttttccc accgttccgc cgcccacgcc tgggatccgc gcaaccaacg tcccgagcta
     1621 tggcaattgt tcaataccaa gatcaagccg ggcgaatcga tgcgagtttt tcccttatcg
     1681 aattggacag agctcgacgt ctgggactat atcttggcgc aagacatccc tgtcgtgccg
     1741 ctttattttg ccaaagagcg ccctgtcgtc aagcgtgacg gcgcgctcat catggtcgat
     1801 gacgaacgcc tgcctttgct tcccggcgaa acgccgcaca tgaagcgcat tcggtttcgg
     1861 acgcttggct gctatcccct gaccggggcg atccgctcgg acgcggggac tctgcccgaa
     1921 attatcgccg aaatgcgtgc ctcgcgaaca tcggagcgcc aaggccgcct gatcgaccat
     1981 gacgaatccg gatcgatgga aaagaagaag cgcgaaggct atttttgatg ctctatgcca
     2041 cgcaagaaat tttgaggctt caatccgaca cggctgccct ccccacgttg cggttgctta
     2101 cttgcggctc tgtcgacgat ggcaaatcga cactgatcgg ccgcttgctt tacgagcaga
     2161 gcctcattcc cgacgatcag ctcgcagcgc tcgaatgcga ctctaaaaag catggcaccg
     2221 atggcgacaa tatcgacttc gcactgcttg tggacgggct ggaggccgag cgcgagcagg
     2281 ggatcaccat tgacgttgcc taccgttacg tctccacgcc aaagcgttct ttcgtcattg
     2341 ccgatacgcc aggacatgag caatataccc gcaatatggc gaccggtgcg tccggcgcgg
     2401 atcttgcaat tttgctcgtc gacgcgcgca agggaatttt gacccagacc tgccgccacg
     2461 ccgcaatcgt ctcgttgacc ggcatcaaaa atgtggtgct tgccgtcaac aagatagatt
     2521 tgatcggctt cgaccaggcc tgcttcgata agattagcgc agacttcatt ggctttgcca
     2581 cagcgctcaa gttcaaatcg gtcacgccca ttccgatttc ggcgcgctat ggcgacaata
     2641 tctcaaagcc gagcgcaaac acgccctggt atcgcggtcc tacgctgctc gattatctcg
     2701 agaccatcgg aatcgcggag gaccgtgcca aagagccgtt ccgcttcctt gttcaatggg
     2761 tcaatcgtcc gcatctcgat tttcgcggtt tttcgggggc cgtcgcgagc ggaacgatcc
     2821 gccagggtga cgaactcacc gtcgccagct cgggccgcat gtcaagggtc gcgcgcatcg
     2881 ccaccgcgga tggcgatctt gaaagcgcgt cagctggcga ttccgtgacc cttacgctga
     2941 ccgacgagct ggatatcgcg cgcggcgata ttttgtgcga tccgaagaat cggccggaag
     3001 tggcggagca gtttgccgcg cacctttttt ggatgagcga cgataagctc ttgcctggcc
     3061 gctcctatct tttgaaaacc ggcgcaagga ccgttccagt ctcggtgacc gagctgaaat
     3121 accggctcga tgttaattct ctcagtgaac tcgcagcaaa aactttgcgc cttaatgagg
     3181 tcggtttttg caatcttgct gccaccacgc cgattgcctt cgacccctat aaaaaaaacc
     3241 aagcgaccgg ggcttttatt ctgatcgacc gcctcactaa tgctaccgcg gcggcgggga
     3301 tgatcgcgtt cgggcttcgc cgtgcgacca acatccatct tcagggcttg agcgtcagca
     3361 aactcgaccg ctgccaaatc aagcatcacc agccggcagt actgtggttc accggtcttt
     3421 cgggctccgg caaatcgacc atcgccaatc tcgtcgaatc tcggctctgt gcaaacgggg
     3481 tccacaccat cctgctcgat ggcgataacg ttcgccatgg gcttaataac gacttgggtt
     3541 tcaccgaagt ggaccgagtc gaaaacattc gccgcattgg cgaggtggca aagctcatga
     3601 cgcaggctgg ccttgtcgtg ctgtgctcct ttatctcgcc ttttcgggcc gagcggcgga
     3661 tggtacgcga acttcttgac cgccaagaat tcatcgaaat ttatgttgca acgccgcttg
     3721 aggattgcat caaacgggat cccaaggggc tttacaaaaa agcgatggcc ggcgagatca
     3781 aaaattttac cggtgtcgat caggccta
//
LOCUS       DATMPU010000050         6700 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_1591250,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000050 DATMPU010000000
VERSION     DATMPU010000050.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 6700)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6700)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6700
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1591250"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            38..448
                     /locus_tag="VNY06_01100"
     CDS             38..448
                     /locus_tag="VNY06_01100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX13471.1"
                     /translation="MSNQVMTEDEQIDFIKQGGEFGQLVSKLTIRAIDAETLSESLQT
                     TVHLQEAQILSLQRRLANAETQRDDANRDVGLMNDAENKIAAILRETRAQRVATPAVR
                     HEARQELTGAPMHPRELQVVRRTAEENESRERRA"
     gene            787..936
                     /locus_tag="VNY06_01105"
     CDS             787..936
                     /locus_tag="VNY06_01105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX13472.1"
                     /translation="MAVWSGHVAAIVECLGDGTARLYDPNSGGGLTRIDVRPLPALIV
                     DPRGG"
     gene            complement(1324..1488)
                     /locus_tag="VNY06_01110"
     CDS             complement(1324..1488)
                     /locus_tag="VNY06_01110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX13473.1"
                     /translation="MTTPEWLRLIADRIELSPVLILASNRENAKWQHKLSVERRNLLA
                     SVLRAAAERQ"
     gene            complement(1504..1719)
                     /locus_tag="VNY06_01115"
     CDS             complement(1504..1719)
                     /locus_tag="VNY06_01115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX13474.1"
                     /translation="MRTQSDRGADLAVGRLIEAYDQYIELLATSEAALLGLAYAHGFH
                     VPESKVKLGEQMRARIAELKTTVLNKS"
     gene            complement(1733..1945)
                     /locus_tag="VNY06_01120"
     CDS             complement(1733..1945)
                     /locus_tag="VNY06_01120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX13475.1"
                     /translation="MTLRYVRAKGPMYTDMLNNMRQTPLCPLGDAILVWVNEGDFWRL
                     TPTDVETEAKDRAEYERLKAKFGFKE"
     gene            complement(1942..2211)
                     /locus_tag="VNY06_01125"
     CDS             complement(1942..2211)
                     /locus_tag="VNY06_01125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX13476.1"
                     /translation="MSVICNCGAEVPEPGMYCSATTIFICQGREPKPGCGHLLTAEER
                     HWYGSCCEDCEQRWSDRIGEWCDGARDAELDAMFGSNGQSAGNLK"
     gene            complement(2233..2499)
                     /locus_tag="VNY06_01130"
     CDS             complement(2233..2499)
                     /locus_tag="VNY06_01130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX13477.1"
                     /translation="MSAPIALVPKPRAADDVLESVTRLLRDSLEQAERGEIDTVIIIL
                     GHPDGEWSDRCSSTEKLSAAIGRLEITKAEWVVQYLRGRNSGDS"
     gene            complement(2496..2795)
                     /locus_tag="VNY06_01135"
     CDS             complement(2496..2795)
                     /locus_tag="VNY06_01135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX13478.1"
                     /translation="MGEPSIMRQLEIALASSGGSERLFRVHCRPIVAKIESDAKMIAA
                     LREALGVVLRAFKAHLAMQYAPAADRAGLIAARDTAVAALAAFATTDEQTVEESK"
     gene            complement(2810..2995)
                     /locus_tag="VNY06_01140"
     CDS             complement(2810..2995)
                     /locus_tag="VNY06_01140"
                     /inference="COORDINATES: protein motif:HMM:NF021072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheV family putative metal-binding protein"
                     /protein_id="HWX13479.1"
                     /translation="MTNPKIKPCPKCGAPDNLAVYRYDNDTRHVECLDCDYLGPGEGS
                     VRAAIIAHNERQQTTVP"
     gene            3168..3443
                     /locus_tag="VNY06_01145"
     CDS             3168..3443
                     /locus_tag="VNY06_01145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX13480.1"
                     /translation="MPVSQVGHGLSDIIQGFEDFLWGKISDVLSPGVYRLKERLCNIG
                     WWIALGEKSIDNVSRHGQLRVVGGLVGCFSFGYFRVLFVVLKCRGRR"
     gene            complement(3578..4045)
                     /locus_tag="VNY06_01150"
     CDS             complement(3578..4045)
                     /locus_tag="VNY06_01150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX13481.1"
                     /translation="MKEPVDHILRPRLPWRSPNEPAITECGYDASKVKTLTRAEFFQR
                     EKELGQRRCVMLTCMTCADTARRWGTWEDDPRLAVQREAEWERQAPYYRRCPSDERGT
                     VLKDELVAIAGLIEAHRDDFDARILEIRQRRKWNEKKAAMTVKAPPRREPGGL"
     gene            complement(4042..4440)
                     /locus_tag="VNY06_01155"
     CDS             complement(4042..4440)
                     /locus_tag="VNY06_01155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX13482.1"
                     /translation="MTDLIERLEKATGPDRHLNALIYLDVVEPSLRASWRAPNGVGPE
                     GYYARIGPRYTESIDAALTLVPSSAQWSLEADTAWVRWMGKDDVEEAQGGFNGREGTC
                     TALALCIAAIKARAAISNQRQSGRMSGDSR"
     gene            complement(4457..4948)
                     /locus_tag="VNY06_01160"
     CDS             complement(4457..4948)
                     /locus_tag="VNY06_01160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX13483.1"
                     /translation="MIDLDATGRLTKWFVWSVDHLPMTNGYIDSGKDDVPDTAKSGAW
                     YVEHGTTLCHVFWAILWVPLLGAAFAGFMFSMVIMMHVEAHYEFVEKHPFANPVIQVA
                     SYFIPEAFLLGVALLVGIIILALMGGSKTGFFSLLWQYLKGIKQRICPLVHFDGAHMQ
                     PAE"
     gene            complement(4995..5285)
                     /locus_tag="VNY06_01165"
     CDS             complement(4995..5285)
                     /locus_tag="VNY06_01165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX13484.1"
                     /translation="MVTDQEIADAEAVLRAATTAYLEAKEKVHRLKETQACEEYKVGI
                     GMIVSDRRGRTGVVCRVSPTYGGKPWVYAKQIKKDGSRGERELSMYSDWTVV"
     gene            complement(5574..5777)
                     /locus_tag="VNY06_01170"
     CDS             complement(5574..5777)
                     /locus_tag="VNY06_01170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX13485.1"
                     /translation="MNLPADKIPTDAPSFRKWADAACPQWPGELQVLAYRAWQWLEGM
                     SFRSAADHEAFMAGYKKFMGAAS"
     gene            complement(5774..5998)
                     /locus_tag="VNY06_01175"
     CDS             complement(5774..5998)
                     /locus_tag="VNY06_01175"
                     /inference="COORDINATES: protein motif:HMM:NF024252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HWX13486.1"
                     /translation="MADFRKEFAKRLRFTREMRELTQAELGSRSDLDQTHVAHFEAGS
                     RLPGAENLKALAKGLMVSSDYLLGLSERVQ"
     gene            6163..>6700
                     /locus_tag="VNY06_01180"
     CDS             6163..>6700
                     /locus_tag="VNY06_01180"
                     /inference="COORDINATES: protein motif:HMM:NF019146.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5131 family protein"
                     /protein_id="HWX13487.1"
                     /translation="MMDHRYHKVEWGPHGERKRTSTANWQKPRQWAKSAKGTGKRPRV
                     FCASLADVWDNQVPVEWRTDLFHLIRETPKLDWLLLTKRPENIEKMLPRAIGETELWP
                     WPNVWLGTTAEDREQYDRRWSRLREIPARVRFISYEPAIGPLNRPHAWDLGEKPDWII
                     CGGESGKSARMMEPAWARW"
BASE COUNT         1319 a         1967 c         1866 g         1548 t
ORIGIN      
        1 cgcattatcc acaaaccaca cacgggggag ttatcaaatg tccaaccagg tcatgactga
       61 ggacgaacag atcgatttca tcaaacaagg cggcgagttc ggccagcttg tttcaaagct
      121 cactattcgc gcgatcgacg ccgagacgct ttcggaatct ctccaaacca ccgtgcatct
      181 acaagaggca cagattctca gcctgcaacg ccggctggcc aatgccgaaa cgcaacgcga
      241 cgacgccaac cgcgacgtgg ggctgatgaa cgatgccgaa aacaaaatcg cggctattct
      301 tcgcgaaacc agagcacagc gggtcgccac gcctgcggtg cgccatgagg cgcggcagga
      361 attgaccggg gcgccaatgc acccgcgcga actgcaagtg gtgcgccgca ctgccgagga
      421 aaacgagtct cgcgagcgcc gggcttgatc cgagccatag cggccgtcct gatcctcagt
      481 attgccgccc cggccgaagc cgccggccgc aggcatcgtg cccctgaatc cagctttgcg
      541 gcggcgtggc acgcggttac tggcgcgtta tccggcgtag cccactccat tcagacggcg
      601 gcggcagagg ccagcccacg ggcttcctac ggcgaaggcg aagtcgttcc acacccgtca
      661 ggctgtcccc gcacggcctt ctgcggctgt ggcgtggccg tgaaggtatt cggcgaaccg
      721 cggcgcgacc tgtggccatc aagtgcttgg aggcgcttcc cgcgcgcctc atgcgcgccg
      781 ggccgggtgg cggtttggtc cgggcatgtc gcggcgatcg tggaatgcct cggggacggc
      841 acggcgcggc tctacgatcc gaacagcggc gggggactca cgcggattga cgtgcggccg
      901 ttgccggcgc tgatcgtcga tccgcgcggc ggctaatctt gctttagtcg gccgccgcta
      961 aggcggtaac ctctttcggg ttttcattgc gccattgctg ccagctttcg tcggtgagtt
     1021 tgtgcctatt ccattccggc aaatctggct tccgggtgaa aggtgttctt tgacgtggcc
     1081 agcatcgaaa gcatagctgt ccgaacttct cagagtagcg aagatgttcc gagcaaaagt
     1141 gcaacccaca gccacgatcc tcgccgtccg tatttatcat tccgcagaca aacgaaaggc
     1201 cgcgatcaat gcgctcgctg caatcaggat gatcgcacag agccggaaca ccgtagccga
     1261 tgtctcgtcc gtcttttcct gtgcctacag cccaactcat tttttgcttc ccggttaact
     1321 tgtctactgt cgctcggcgg cggcgcgtag aacagacgca agtaagttgc gccgctctac
     1381 cgaaagtttg tgctgccact tggcgttctc tcgattgctg gcaagtatca gcacgggact
     1441 tagctcgatg cgatctgcta tcaatctaag ccattctggc gtggtcattg ttcagcgccc
     1501 cgattaactc ttgtttagca cggttgtttt caattcggcg atccttgcgc gcatttgctc
     1561 gccgagcttc actttgcttt ccgggacatg gaagccatga gcataggcga gcccgagaag
     1621 agcagcttcc gaagtggcca gtagttcaat atattggtca taggcttcga tcagccttcc
     1681 aacagccaaa tctgcgccac gatccgattg tgttctcacg acagttctct tgttactctt
     1741 taaatccgaa cttggccttg agcctctcat attcggcgcg gtcttttgct tccgtctcaa
     1801 catcggtcgg cgtcaatcgc caaaagtcgc cttcgttgac ccaaacgaga atggcgtcgc
     1861 cgagcggaca caatggcgtc tgtctcatgt tgttaagcat gtcagtatac atggggcctt
     1921 tcgcgcgaac gtatctcagt gtcattttaa gtttcctgct gattggccat tactgccgaa
     1981 catagcatca agttcggcat cgcgggctcc gtcgcaccat tcgccgatgc ggtcgctcca
     2041 ccgctgctcg caatcttcac agcatgaacc gtaccaatga cgttcctcgg ctgtgagcaa
     2101 atgaccgcag cccggcttcg gctcacgtcc ttggcagatg aaaattgtcg tcgcgctaca
     2161 gtacatccct ggctcgggca cttctgcacc acaattgcaa atgacggaca tgggcacctc
     2221 cccgctgatc gcttaactgt cgccgctatt tcggccgcga agatattgaa ccacccattc
     2281 ggctttagtg atttcaaggc gaccaattgc cgctgaaagt ttttcggtgc tgctacatcg
     2341 atccgaccat tcgccgtcag gatggccgag gatgataatc accgtgtcaa tctcgccgcg
     2401 ttcggcctgt tcgagtgaat cgcgtagtag ccgcgtcacg ctctcgagca catcgtctgc
     2461 ggctctaggt tttggaacca aggcaatggg cgcgctcatt tgctttcctc gactgtctgc
     2521 tcatctgtcg ttgcaaatgc ggcgagcgcc gcgacggcgg tatcgcgcgc tgctatcaag
     2581 cccgctctgt ccgcagcagg cgcgtattgc atcgccaggt gcgctttgaa cgctctaagg
     2641 acaactccaa gcgcctccct caacgccgcg atcatcttcg cgtcggattc gatcttggcg
     2701 acgatggggc ggcaatgcac tcgaaatagt ctctcgctac ccccactgct tgctagggca
     2761 atttcaagct gtcgcattat ggatggctcg cccattttct gcctgcttgt cagggaacag
     2821 tcgtctgctg gcgttcattg tgggctatga ttgcggcgcg caccgacccc tcgcccggcc
     2881 caagataatc gcaatcgagg cactcaacat gccgagtgtc gttgtcataa cgataaacgg
     2941 ctagattgtc tggcgcgcca cacttcggac agggttttat ttttggattt gtcatttgtt
     3001 gcttccgtta gacttgattt ccacaatctc aaatctactc agcttgacgc cagaaacacc
     3061 cttccaaaaa tccagcaact ccttttgcgc ggcttctcgg gtcggatacg tctcggtgcc
     3121 ccaacgcttc ccattctgat tttgaatttc aaagtaagtc atcgtatgtg cctgtctctc
     3181 aagtcggtca cggtctgtct gacatcatcc aaggctttga ggatttcttg tggggtaaga
     3241 tcagcgacgt tctctcgcca ggcgtctatc gtttgaaaga gcgcttgtgc aatatcggtt
     3301 ggtggatcgc gcttggtgag aagtccatag acaatgtgtc ccggcacggt caactccgtg
     3361 ttgttggggg tttagtcggc tgcttttcgt tcggctattt ccgcgttctc tttgtggtct
     3421 tgaaatgccg cggacgccga tagcgacgtg aagtggcagg agacccaacc gcactcgcac
     3481 ctagcgacga taccgttgtc cgttcgcttg agcgttccgc cttggagccg gtgttgcatc
     3541 tgaatctcca tgctgttcgg cggttagtgc ctgtttgtca tagaccgccc ggctctcggc
     3601 gcggcggggc cttcacggtc atcgcagctt tcttttcgtt ccacttgcgc cgctgcctga
     3661 tctcaagaat gcgcgcatcg aagtcatcgc ggtgggcctc gatcaagccc gcgatggcaa
     3721 ccaactcgtc ctttaggacg gtgccgcgtt cgtcagaggg acagcgccga taatatggcg
     3781 cctgcctctc ccattcggct tcgcgctgca cggccaggcg cgggtcatcc tcccacgtac
     3841 cccagcgcct cgccgtgtcg gcgcacgtca tgcaggtcag catcacacag cgacgttgcc
     3901 caagttcctt ttcgcgctgg aagaactcag cgcgggtaag cgtcttgacc tttgaggcat
     3961 catagccgca ttcggtgata gccggttcgt ttggcgagcg ccaaggcaag cggggtcgca
     4021 ggatgtgatc gactggctct ttcatcgcga atctcccgac attcgtcctg actgtcgctg
     4081 attgctgata gcggcgcgcg cctttatggc tgcgatgcag agtgcgaggg ctgtgcaagt
     4141 gccttcgcga ccattgaagc cgccctgcgc ttcttctacg tcgtccttcc ccatccagcg
     4201 gacccatgcg gtgtctgcct ctaggctcca ttgtgcgctc gacgggacca gggttagcgc
     4261 agcgtcgatg gactcggtgt aacgcggccc tatgcgtgcg tagtagcctt cggggcctac
     4321 cccgttgggg gcgcgccagc ttgcccgcag agacggctct acaacatcga gatagatcag
     4381 cgcattgagg tgccgatctg gtcccgtcgc cttctctaat cgctcgatca aatcggtcat
     4441 ggcgattaac cccgtgttac tcggctggtt gcatgtgcgc gccgtcgaaa tgtacaagag
     4501 gacatatccg ttgtttgatg cctttcagat attgccaaag cagcgagaag aagccggtct
     4561 tgctgccgcc catgagagcc aaaatgataa ttccgactag gagggcaacc cccaacaaaa
     4621 atgcctctgg gataaaatat gaggccacct gaattacagg attggcaaac gggtgtttct
     4681 cgacaaactc gtagtgggct tcgacgtgca tcataatgac catcgagaac atgaacccgg
     4741 caaaggcggc gcccagcagc ggaacccaga ggatggccca gaatacatgg cagagtgtgg
     4801 tgccgtgttc gacgtaccac gcgccacttt tggcggtatc tggcacatcg tcttttccag
     4861 aatcgatata gccgttcgtc ataggtaggt ggtcaactga ccatacaaac cattttgtta
     4921 gtctgccggt cgcgtccaga tcgatcatgt tcttttcctt tggataaacg ggcactaacg
     4981 acgcatttgt cgtttcaaac gacagtccaa tcagagtaca tggaaagctc gcgctcaccc
     5041 cgcgagccgt ctttctttat ttgcttcgcg tagacccaag gcttgccgcc atatgtgggg
     5101 ctgacgcggc agacgacgcc agtgcgaccc cttctatcag aaacaatcat gccgatgccc
     5161 accttgtatt cctcacaagc ctgagtttct ttcaaacggt gtaccttttc tttggcttcg
     5221 aggtaggcag ttgtggccgc tctaagaaca gcctcggcat ctgcaatttc ttgatccgtg
     5281 accatcacgc caccttctcg ctgctctttt ccatccgcat ccgctgcacg gtatgggtgt
     5341 tctcccggct ggcgcggctg tagtccatca gattcatgac cgttgccaat ctagaatctt
     5401 gcggccgtca cgcgttgcga tcaaacggcc gaactttact cggcatagtt ttctgccaac
     5461 gagccatttg acttgccgta tcctgccgcg ctgatattcg tacatcattg cggttcctgg
     5521 atttatcttg tcaccgcctg cggcgcgagc aagaacgcta agttgcagcg aagtcatgat
     5581 gccgctccca tgaatttctt gtaccccgcc ataaacgcct catggtccgc agccgaccga
     5641 aacgacatgc cttccagcca ttgccaagcg cgataggcga ggacctgtag ttcgccgggc
     5701 cattgcgggc aggcggcatc ggcccatttc cgaaatgacg gggcgtcggt tggaattttg
     5761 tcggcgggga ggttcactgt acgcgctccg aaagtccaag aagataatcc gacgaaacca
     5821 tgaggccctt ggcgagcgcc tttaggttct ctgcccccgg aagccgacta ccggcctcaa
     5881 aatgagccac atgggtttgg tccagatcgg aacggctgcc caattcggct tgggttagct
     5941 cccgcatttc acgggtgaac cgcagccgct tggcaaattc cttccgaaag tcagccatat
     6001 caaacctcac gtttccgtca taatatgtgg tattacgggt tagtcaatag ggctagcaaa
     6061 attatttcgg gcgctaaacc tcagttagcc ggcagaaacg tcgctgcgat atggcgactg
     6121 agcgggcgcc ggaatcttcg cgatcattgc tatgccgaag ccatgatgga ccaccgctat
     6181 cacaaggtag aatggggtcc acacggcgag cgcaagcgca ccagcacggc gaattggcag
     6241 aagcctcgac agtgggctaa gtcggcgaaa ggcactggca agcgcccgcg cgtgttctgc
     6301 gcctcgttgg cggacgtttg ggataatcag gtcccggtcg agtggcgcac cgatcttttc
     6361 cacctgattc gggagacgcc gaagcttgat tggctgttgc tcacgaagcg gccggagaac
     6421 atcgaaaaga tgttgccgcg cgccattggc gagacggaac tatggccctg gccgaatgtg
     6481 tggctcggga cgactgccga agatcgcgag cagtatgacc gccgctggag tcggctgcgc
     6541 gagattccgg cccgcgtgag atttattagc tacgagccgg caataggccc gctcaatcgt
     6601 ccgcacgctt gggatttggg cgaaaagcca gattggatca tctgtggcgg cgagagcggt
     6661 aagagcgcgc ggatgatgga gccggcttgg gcgcgttggg
//
LOCUS       DATMPU010000051         3475 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_1591255,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000051 DATMPU010000000
VERSION     DATMPU010000051.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 3475)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3475)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3475
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1591255"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(897..3068)
                     /gene="scpA"
                     /locus_tag="VNY06_01185"
     CDS             complement(897..3068)
                     /gene="scpA"
                     /locus_tag="VNY06_01185"
                     /EC_number="5.4.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018260523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004494 - methylmalonyl-CoA mutase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HWX13488.1"
                     /translation="MSCNTSRVPNLSTLPFVSIPVEVDSIPAAPWLTPEAIPVAPIYH
                     HRDIAGLDFLDGFPGIAPYLRGPYPAMYPMRPWTIRQYAGFSTAEESNAFYRSNLSAG
                     QTGLSIAFDLATHRGYDSDHPRVEADVGMAGVAIDSIYDMRALFAAIPLGEVSVSMTM
                     SGAVLPVLALYILAAEEQGVAKAKLSGTIQNDILKEFMVRNTYIYPPEPSLRIVSDIL
                     AYTAQRMPNFNAISISGYHMQEAGATADLELGYTLADGIEYVRAGLAGGLQIDQFTPR
                     LSFFFATSMNFFMETAKLRAARLLWAKLMQAFLPANERSLALRTHAQTSGWSLAAQDI
                     FNNIVRTTIEAMAATQGHVQSLHTNALDEALALPSNFSARIARNTQLFLQQESGTTRV
                     IDPWGGSYYVEHLTSALAARASAHIAEIEEAGGMAKSIGAGIPKRRIEEAAAKTQARI
                     DSGTDTVIGVNSFKPAKEENIQILKVDTTAVRLLQIAKLTRLRAERNPGEVARALNAL
                     TEGARGKANLLTLAVEAARAKATVGEISFALEKVFGRHNAETITVSGVYAREAGRNSP
                     AFLRSKDLVQAFAENEGHPPRILVAKIGQDGHDRGQKVIASALRDMGFDVEIGPLFAT
                     PDEVARQAVASKVHVLGISSLAAGHLTFVPQLRAALTREGRADIMIVVGGVIPPQDFD
                     ALREAGVTAIFPPGTVVPEAAARLIELLNDRLGYQQKEMIE"
     gene            complement(3155..>3475)
                     /locus_tag="VNY06_01190"
     CDS             complement(3155..>3475)
                     /locus_tag="VNY06_01190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX13489.1"
                     /translation="LIGVSLKGDDGIYLVNVTTHRGDCDKDYQWIIMVSGGRVSSAGD
                     TPMDASGQINSRGVVTLRFERFGQVANVTGRVAKDSGSGTWNSATLQCSGSWRATRRG
                     WADR"
BASE COUNT          729 a          997 c         1000 g          749 t
ORIGIN      
        1 aagcgtttcc ctcgcatcgg gatatccaac ggagggctac tactgcacca acgcctcaaa
       61 cgtcctgcag agctatcgcg gtcttcgcgg cggcaagcct ccattatgcg gtggagggag
      121 cggcgcgctg ctattcggtc aattccatcc agtgcggcac tgccacggcg actcagagct
      181 acgcccaaac cctctatcaa ggcccgagca gtccggcatt ttcggcatca aaaccttcat
      241 gcggccatca agtcagcgga acgctcaact atgtactcag cgcaggggtg gcgcaatgga
      301 caataccgtt gtccgcaacc gcctgctttc cttaagtgta tttggcagtg tggccgtcat
      361 cctctaaatt gcccgccgtc ggccgccagc ctgttcatgg gcttttcccg ggcaaaatag
      421 gaatcagagt ttcggttcca agaagagctt atgttatccg tctgccgttt cagagcatca
      481 cgattcctcg ccatggtcgc gcaacacatg gtattcgtgt agcgacttcg actccgtcag
      541 cgaaacttac atcgagaatg ccttccggca acgggtaggg cgatcaaggc gccgccgccg
      601 ccctcgttgc aattgcacca aaagatcgat cgatcttacc gaatcgaaga cgggtcgctg
      661 tcttcggcct tgtaaggttt ttggccccga cccgacaaaa atgtttcact gaaacatttt
      721 ggtaaggttc tctccgccgc aaacgagccc ccggcttgtg ggattctgct gggcgtcgaa
      781 cacgtttgct gatcggccgc cgttcctcgg cagctcagtc attccgaaat agcccggcga
      841 gagtcatgga caacgggggt tggtataagt cagcttaagt gacagttctg cggttgttat
      901 tcgatcatct ctttctgctg gtagccgagg cgatcgttta atagctcgat caggcgcgca
      961 gccgcttccg gaacgaccgt acccggcggg aatatcgccg ttactccggc ctctcttaat
     1021 gcgtcgaaat cttgcggcgg gattacgccg cccactacga tcatgatgtc tgcgcgacct
     1081 tcgcgcgtga gcgccgcgcg caattgcggc acgaaggtca gatgcccggc ggcaagcgaa
     1141 gaaattccta aaacatgaac cttgctcgcc accgcttgcc gcgcgacctc atcgggtgtt
     1201 gcgaataagg gcccgatttc gacatcgaag cccatgtcgc gcagcgccga ggcaatcacc
     1261 ttctggcccc ggtcatgccc atcttgtcca atcttggcga caaggattcg tggagggtgg
     1321 ccttcgtttt ctgcaaacgc ctgcaccaaa tccttgctgc gaaggaaagc aggagaattt
     1381 ctgcccgcct cccttgcata gacgccagaa acggtgatgg tctcggcatt atggcggcca
     1441 aagacttttt cgagcgcaaa agatatttcg ccgacagttg ccttggctcg cgccgcttcg
     1501 accgcaaggg tcaagagatt ggctttgcca cgtgcacctt cggtcaaggc atttagcgca
     1561 cgagcgactt cgccagggtt ccgctctgcc cgcaagcgtg tgagcttagc gatctgcagt
     1621 aagcgaaccg cagtcgtgtc gaccttcaaa atctgaatat tttcctcctt ggcgggtttg
     1681 aaactgttaa cgccgatcac cgtgtccgtg ccgctgtcga tgcgcgcctg ggtcttggct
     1741 gctgcctcct cgatgcgccg ctttgggatt cctgctccta tggatttcgc cattccgccg
     1801 gcttcttcga tctccgcaat atgcgccgag gccctggcag cgagcgcgga agtcaggtgc
     1861 tcgacatagt aagagccgcc ccatgggtcg atgacgcgcg tcgtgccgct ttcctgttgc
     1921 aggaagagct gggtgttgcg cgcaatgcgt gctgagaaat tgcttggcag cgctaacgct
     1981 tcatcgagcg cgttcgtgtg caaggattgc acatgacctt gggtggcagc catcgcctcg
     2041 attgtcgtgc gcactatgtt gttgaaaata tcctgcgcag cgagagacca gccggaagtc
     2101 tgtgcatgag tgcgaagggc tagggacctt tcgttggcgg gaagaaatgc ttgcatgagc
     2161 tttgcccaaa ggagccgggc ggcgcgaagc ttcgcagtct ccatgaagaa attcatcgat
     2221 gtcgcaaaga agaaggacaa ccttggcgta aactggtcga tctggagtcc gcccgcaaga
     2281 ccggcacgga catattcgat cccatccgct agcgtgtagc cgagttcgag atcggcggtc
     2341 gcacctgcct cttgcatgtg atagccggag atcgagatgg cgttgaaatt gggcatacgc
     2401 tgcgcagtat aggctaaaat atcggaaacg atgcgcagcg agggctccgg cggatagata
     2461 taagtgttgc ggaccatgaa ttccttgaga atatcatttt gtatcgtgcc gctgagtttg
     2521 gcctttgcca ctccttgctc ttcggcagcc aagatataga gcgccagtac cggcaggacc
     2581 gcgccgctca tcgtcatcga aacggaaact tcgccaagcg gaattgcggc aaacagcgcg
     2641 cgcatgtcgt agatcgagtc gattgcgaca ccagccatgc caacatcggc ttcgacccgt
     2701 ggatgatcgg agtcgtagcc gcgatgggtg gcgaggtcga aagcgattga aagcccggtc
     2761 tgcccggcag agagattgct tcggtagaag gcgttcgact cctcggcggt ggaaaaaccc
     2821 gcatattggc ggatggtcca aggccgcatg ggatacatgg ccggataagg gccgcgcaaa
     2881 taaggcgcta tgcccggaaa accgtcgagg aaatctaggc cagcgatgtc cctatggtga
     2941 tagatcggcg caacagggat agcttcgggt gtcagccagg gtgcggcagg gatagagtcg
     3001 acttcgaccg gaatcgagac aaatggcagc gtggacaaat ttggaacgcg gcttgtgttg
     3061 cagctcatgg gagagcgctc cgggcgagag gcaccgccta ggatatggtt ctgcgcgcgc
     3121 taaacggcca tctcggccct aggagccggt cgctttaccg gtcagcccag ccccgccttg
     3181 tggccctcca cgagccagag cattgcaggg ttgccgagtt ccaagtgccc gaaccagagt
     3241 ccttcgcaac ccttccggtt acgttggcaa cttgcccgaa acgctcgaac cgcaaggtga
     3301 caacaccgcg agaattgatc tggcccgatg cgtccatggg cgtatccccc gccgagctga
     3361 cccgtccgcc ggaaaccata ataatccatt ggtaatcttt gtcacaatct cctcggtgcg
     3421 tcgtgacatt aaccaaatag atcccatcgt cgcccttcag gctgacgcca atgag
//
LOCUS       DATMPU010000052         6151 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_2830141,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000052 DATMPU010000000
VERSION     DATMPU010000052.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 6151)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6151)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6151
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2830141"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(139..372)
                     /locus_tag="VNY06_01195"
     CDS             complement(139..372)
                     /locus_tag="VNY06_01195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014892820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine rich repeat-containing protein"
                     /protein_id="HWX13490.1"
                     /translation="MPNRLLSAGLIASFLLAAPVKSQEMADIKTYCMSDIERLCKGIE
                     PGGGRILKCLKANKKEMSVGCAQALQKLKAKKQ"
     gene            complement(804..3053)
                     /locus_tag="VNY06_01200"
     CDS             complement(804..3053)
                     /locus_tag="VNY06_01200"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018408859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HWX13491.1"
                     /translation="MAALGDSDRTDSTALAEVLLPLAIDHPYSYLIPHGTSIAPGAFV
                     EVPLGTKLTDGVVWQVSARASGNTNLKPIVNRRDLSPLSTNLRKFIDWVARWTLSPRG
                     MVLRMAIGAPLHARPELLRVGVRFNGPAPRRMTPARARVLAAAEGGIAFSKAALAEAA
                     ACSAGVIDSLVDEGTLETIALPPELVALPCDPAYATPVLEAAQQSAALELAARARARK
                     FSATLLEGVTGSGKTEVYFEAVAAALAMGRQSLILMPEIALTAQFLERFAARFGARPG
                     EWHSGVSAGKRARLWGAVAKGEAKAVIGARSALFLPFNDLAAIIVDEEHEPAYKQEDG
                     VAYHARDMAVVRGQIENCPVVLVSATPSLESRVNAAHGRYGHSRLASRFNAKALPALT
                     AIDLRTDAAPRGKWIAPKLASEISKTLATGEQALLFLNRRGYAPLTLCRACGHRFNCP
                     NCTAWLVEHRFRRALMCHHCGHVERKPAVCPSCEAVDSLTACGPGIERLAEEAAELFP
                     GAKILTLSSDFPGSTERLRAEIDAISGGDCDIVIGTQLVAKGHNFPYLSLAGIIDADV
                     GLTTGDPRAAERTFQLLQQVTGRAGRFETAGRAFIQTWQPEHPVMRALLSGDSERFYE
                     EEIHQRRRAGLPPFGRLAAIVVSAKDGALAQAHARALVQTAYALPPSDSWSLPPPGSP
                     PNADELTLLGPAEAPIAVIRGRYRFRLLVRAPRSADLQAFLRAWLAAGPKEKGSLRVA
                     VDVDPQSFL"
     gene            complement(3181..3896)
                     /locus_tag="VNY06_01205"
                     /pseudo
     CDS             complement(3181..3896)
                     /locus_tag="VNY06_01205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003607015.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
     gene            4263..5672
                     /locus_tag="VNY06_01210"
     CDS             4263..5672
                     /locus_tag="VNY06_01210"
                     /EC_number="2.7.7.13"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020174665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate
                     guanylyltransferase/mannose-6-phosphate isomerase"
                     /protein_id="HWX13492.1"
                     /translation="MKILPVIMCGGAGTRVWPESRESLPKQFISLIGSLSTFQMASEM
                     LSDSVFETPVVISNHDYRFLVAEQLEKIDRKARIVLEPVRRDSGPAVAVAAELALQSG
                     PETIVAVLAADHVVRDQRGFVELCKKAAEAAALSYIVTLGIKPDEPATGYGYIRPGKA
                     VTPDGAVLKVEAFVEKPDAATAETYVAENYLWNSGNFIFRADVMQEELRNFEPNMASA
                     AAEAVAQAKQDLNFLVLDAEAFGRAPKKSIDYAVMEHTAKAAVVPADIGWSDVGNWRA
                     VWQLSDRDQLGNSIRGRGIVMNASNVHVRSEEHLTTVVGVDDVIVVTTQDAVLVLNKA
                     YGDNVKQLVDQMKSEKRPEAMEHKRSYRPWGYYQSVDYGARYQVKRIVVKPGARLSLQ
                     KHFHRAEHWIVVKGTAEVTRDNEVHLVHENESIYLPIGCVHRMANPGRIDLELIEVQT
                     GSYLGEDDIVRLQDVYNRG"
     gene            5727..>6151
                     /locus_tag="VNY06_01215"
     CDS             5727..>6151
                     /locus_tag="VNY06_01215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020174671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX13493.1"
                     /translation="MVRQISSLPFARNFLTVCAAVAAAGASLGPAAARPLVPAERQYI
                     PYDGVLPACDDPAVFERIQGRFHDREAEFWNTGLEILGFERVKEIGYKTNGEDYIPRR
                     YCSARAYLNDEKSHQVSYSINEDLGIIGFGFGVEWCVSGL"
BASE COUNT         1348 a         1702 c         1869 g         1232 t
ORIGIN      
        1 aagcatgctt gtgctcgcct atagattacg tgagcggcac tctggcgctc aaatggcgga
       61 gaaaggtagc cacatctagc agcggcgaga tgggctcaaa gaagtgccgg agggcgaccg
      121 ctctccggca gcagtgggct actgcttttt cgccttgagt ttttgcagcg cctgcgcgca
      181 gccaacgctc atttccttct tattggcttt gagacatttc aaaatccggc cgcctccggg
      241 ttcgatgcct ttgcaaagcc gctcaatatc gctcatgcaa taggttttga tgtccgccat
      301 ttcctgtgat ttgacggggg ctgcgagaag gaatgaggcg atgaggccgg cggacaaaag
      361 gcggttcggc attttcttct ccctatattc gtccggcgac gcccctaccg cgccggaaca
      421 attctctaac gcagccgatc cttagcatta gagttttgcc aagtatacat cgcaaaaagc
      481 tctgcggatc gacatctatc gcagccttaa gtctcgcaaa tagtgacacg gaatgcccca
      541 tttgttcttg aggtgctcca tgctgccgtc ctgaagcatc gccccaagcg cgattcacgg
      601 ccgccaccag agccggctca ttctttccaa acccgattcc gagcagttgc gggtcgtccg
      661 gaacttgcgc aacgatgcgc agggcagggc gcttcgccac cagccatgct gccaccggag
      721 tgaccttcat accgctgtga tctgccctgc ctcaagatcc gtcatcgccg cttcgatccg
      781 gtcgaagcag cccaagcaat cgctcacaaa aagctctgcg gatcgacatc gacggcgacg
      841 cgaaggcttc ccttttcttt aggcccggcg gcgagccagg cgcgcaaaaa tgcttgcaga
      901 tcggcggagc gcggtgccct aaccagaagc cggaagcgat agcgcccgcg aatgaccgcg
      961 atcggcgcct cggcaggccc gagcagggtc agctcatccg cattcggcgg gctgcccggc
     1021 ggtgggagcg accagctgtc cgatgggggc agggcatagg cggtttgcac gagcgccctt
     1081 gcatgggctt gcgccaaggc accgtctttg gcggagacga cgatcgccgc gaggcgcccg
     1141 aagggaggca ggcccgcgcg gcggcgctga tgaatttcct cctcataaaa gcgctcgcta
     1201 tcgccagata aaagcgcgcg catcaccgga tgctccggct gccatgtctg gatgaaggca
     1261 cggcccgcgg tctcaaatcg cccagcgcgc ccagttacct gctgcaatag ctgaaaagtg
     1321 cgctcggccg ctcgtggatc gcccgtcgtc aggccaacat ccgcgtctat gatgccagca
     1381 agcgaaagat aggggaaatt atggccttta gcgacaagct gggtgccgat cacaatatcg
     1441 cagtcgccac ccgagatcgc atcgatttcg gcgcgaagac gctccgtgct gccgggaaaa
     1501 tcggacgaga gggtgagaat tttggcgccc ggaaatagct ctgccgcttc ttccgcgagc
     1561 cgctcgatac cggggccgca ggcagtgagc gaatcgacgg cttcacagga cgggcagaca
     1621 gcgggtttgc gctcaacatg gccgcagtga tggcacatga gcgcccggcg gaaacggtgc
     1681 tcgacgagcc aggccgtgca gtttgggcaa ttaaaacggt gtccgcacgc gcggcagagg
     1741 gttaagggcg catagccgcg ccggttgaga aacaacagcg cctgttcgcc agtggcgagc
     1801 gttttggaaa tttctgatgc gagtttcggg gcgatccatt tgccgcgcgg tgcggcgtcg
     1861 gtgcgaagat ctatcgctgt gagcgcgggt agcgcctttg cgttgaaacg ggaggcaagc
     1921 cggctatggc catagcggcc atgggcggca tttacgcggc tttcaagcga cggtgttgcc
     1981 gaaacaagca caacagggca attttcgatc tgcccgcgca cgaccgccat gtctctcgcg
     2041 tggtaggcaa cgccgtcttc ctgcttgtag gccggctcat gctcctcatc gacgatgatt
     2101 gccgcgagat cgttgaaagg aagaaacagt gccgagcgcg cgccaatgac ggccttagct
     2161 tcgcccttgg cgacagcgcc ccaaagccgc gctcgcttgc cggcgctgac gcccgaatgc
     2221 cattcgcccg gccgcgcgcc aaagcgtgcc gcaaagcgtt ccaaaaattg cgcggtgagt
     2281 gcgatctcgg gcatgaggat gaggctctgg cgccccatcg caagcgccgc ggcgacagcc
     2341 tcgaaataga cctcagtctt cccggaaccg gttacgcctt caagtaaagt cgccgagaat
     2401 ttgcgggcgc gtgcacgcgc tgcaagctct aatgcggcgc tctgctgcgc ggcttcgaga
     2461 acgggcgtcg catatgcggg atcgcagggg agcgccacca gctcgggagg aagcgcaatc
     2521 gtctcaagcg tgccttcgtc aaccaatgag tcgatgacac cggccgaaca ggctgcagcc
     2581 tcggcgagcg ctgccttcga aaacgctatt ccgccttcgg cagcagcgag aacccgggcg
     2641 cgggcggggg tcatccggcg cggcgcgggt ccattaaagc gaacgccaac gcgcagcagt
     2701 tccggccgag catgcaaagg cgcgccaatc gccatacgca agaccatgcc gcgcgggctc
     2761 aaggtccagc gtgcgaccca gtcgatgaac tttcgcagat tggtactaag aggggagagg
     2821 tcacgccggt tcacgatggg tttgagattt gtattcccag acgcccgggc ggaaacttgc
     2881 cagacgacgc catcggttag tttcgtgcct aaaggcactt cgacgaaagc accgggcgca
     2941 atgcttgtgc cgtgcggaat cagataggaa tagggatgat cgatcgcgag aggcaataag
     3001 acttcggcaa gtgctgtgct gtctgtccga tccgagtcgc cgagcgctgc catttcattt
     3061 cgggggccgc ttacgtcttt ttcgtgccgg tggcctctcg gcgtttcttt gtgcgagcga
     3121 attctcggat tttacgcgtg ctggcggcgc gccggaattt tcatgcaggg gttgcatccc
     3181 gcgccttttg cgcgcgagca tgttgagact ctcaaccata gcggaaaacg ccatcgccgc
     3241 gtagatgtag cctcttggga aatgaaaacc aaatccgtcg gcgattaatg ttgcaccgat
     3301 caacagaaga aagcctagcg cgagcattac aatagttggg ttacgacgaa tgaaattggc
     3361 gagcgggccg gaagcgacta gcatcaacat gactgctata atgacagcgc cgatcatgat
     3421 tggaatatgc tcggtcatgc cgaccgcggt aatgatgctg tcaatggaga aaacaaggtc
     3481 aagaagaacg atctggccag ctgtgactgg aaaggtcgcg cggtgcggcc gcgcgacctc
     3541 ggcagtctcg accggatcga tggtgtgatg aatctctgtc gttgccttga agacgaggaa
     3601 gagcccccca aaatgaggat cagatcacga taggaaaagc tttgcgtgaa aatagtgaac
     3661 aagggtgcgg tgagcctgac ggcatagggc gccccagtga cgaggactag ccgcaaaagg
     3721 agggaaaggc cgattccgat ccgccgtgcc cttgctccct cctttggcgg taatcttgac
     3781 gataaaagcg ctataaagac gagattatcg acgccaagca caacctccat cacggccagg
     3841 gtggcaagtg cgagccacgc cgaaggatcg gcggcgagca ccaggagttg atccatcaca
     3901 agccgttctt cagaaggatt ttcgcgcgtg acgcgttaaa ggcgagtgtg caccgtatag
     3961 gccaaggatt tccggagcaa gatcaagccg cataagcgaa tgggcgcaca ggatgaaaat
     4021 cgcatccctc ccggaacgaa tagcagttcg cctaaaccct cgaccttggc attcgaagct
     4081 attaacaacc gtcataattg tggctacgag cgccgacacg ggcgaccgtt tgcgcagcac
     4141 gaggggctat tcaaggtgac tatcatggtg caatgcgtca aaacacgcgc ggattcgccg
     4201 aatgtggcta tgaaagccat aggtacattg tactgtcatt tcattgtaag gagtaagaag
     4261 acatgaaaat tcttccagtc ataatgtgcg gtggtgctgg aacgagggtt tggccggaat
     4321 cgcgcgaaag tctgccgaag caatttatat cccttattgg ttcgctttcg accttccaga
     4381 tggcgagcga aatgctatcg gacagcgtat tcgagacgcc ggtcgtcatt tccaatcacg
     4441 attaccgttt tcttgtcgcc gagcaattgg agaagatcga tcgcaaggcg cgcatcgtcc
     4501 tcgaaccggt tcgcagggat tccggaccgg cggttgcggt tgctgccgaa cttgccttgc
     4561 agtcagggcc tgaaacaatc gtcgccgttt tggccgccga ccatgtggtg cgcgaccaga
     4621 ggggattcgt tgagctttgt aagaaggcgg ccgaggcggc cgcgctcagc tatatcgtca
     4681 cactcggcat aaagccagac gagcccgcga cgggttatgg ttatatcagg ccgggaaagg
     4741 cggtcacacc agatggagcg gtcttaaagg tcgaggcatt tgttgaaaag ccggatgctg
     4801 cgaccgcgga aacttatgtc gccgaaaatt acctctggaa ctcgggcaat ttcattttcc
     4861 gtgccgatgt gatgcaggaa gagctgcgga attttgagcc aaatatggcc tcggctgcgg
     4921 ctgaagccgt tgcccaggca aaacaggatt tgaatttcct tgtgctcgat gccgaggcct
     4981 ttgggcgggc gcccaagaaa tctatcgact acgctgtcat ggagcacacg gccaaggcgg
     5041 ccgttgtacc tgccgacatt ggctggtccg acgtcggaaa ctggcgggca gtttggcaat
     5101 tgtcggaccg cgatcagctc ggcaattcta ttcgcggccg gggcattgtc atgaatgcgt
     5161 caaacgtcca tgttcgctca gaggaacatt tgacgacagt cgttggtgtc gatgatgtca
     5221 ttgtcgtcac gacacaagat gctgtgctag tcctcaacaa agcgtatggc gacaatgtga
     5281 agcagctcgt cgaccaaatg aaaagcgaga aacgcccgga agccatggaa cataagcgat
     5341 cgtaccggcc gtggggctat tatcaatcag tcgattatgg tgcgcgctat caggtgaaac
     5401 gcattgtcgt gaaaccgggc gcgcgcttat ccttgcaaaa gcattttcac cgcgccgagc
     5461 actggattgt tgtcaagggg acggcggagg tcacaaggga caatgaagtc catcttgtcc
     5521 acgagaacga gtcaatttac ttgccgatag gatgcgtgca tcgcatggca aatcccggcc
     5581 ggatcgatct ggaattgatt gaggtgcaga ctggctctta tcttggcgaa gacgatatcg
     5641 tccggctcca ggacgtctat aatcgcggct gacatgcggc gtttcgcgaa acgcctgcaa
     5701 atggacggat cgaggcattg ccagttatgg taagacaaat ttcaagcttg cctttcgcac
     5761 ggaatttttt gaccgtttgc gcggcggtgg ccgccgccgg cgcgtcccta gggcccgccg
     5821 ccgcgcgccc cctggttccg gcggagcggc agtatattcc ctacgacggt gtgttgcccg
     5881 cttgcgacga tcccgcggtt ttcgaaagga tacaggggcg cttccatgat agggaagccg
     5941 agttctggaa caccggcctt gagatcctgg gcttcgaaag agtcaaggaa atcggctata
     6001 aaacgaacgg ggaggattac atcccccgcc gctattgcag cgcgcgggct tacttaaacg
     6061 atgaaaaatc ccatcaggtt tcctattcga tcaacgagga tcttggcatc ataggtttcg
     6121 gtttcggcgt cgagtggtgc gtgagcggac t
//
LOCUS       DATMPU010000053         3987 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_2519895,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000053 DATMPU010000000
VERSION     DATMPU010000053.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 3987)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3987)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3987
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2519895"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..320)
                     /locus_tag="VNY06_01220"
     CDS             complement(<1..320)
                     /locus_tag="VNY06_01220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012591494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HWX13494.1"
                     /translation="MFQKGWLHAAAICIFLLGIAFEATAAPLFSHNLPSGEALKAIPV
                     VVFGKNSRRSVEEFAAEQKLDPAELRTRFAGSGLIECGDAHGAGQLTVTNNLVTTAAH
                     VFFN"
     gene            856..1821
                     /gene="mdh"
                     /locus_tag="VNY06_01225"
     CDS             856..1821
                     /gene="mdh"
                     /locus_tag="VNY06_01225"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015822219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HWX13495.1"
                     /translation="MARNKIALIGAGQIGGTLAHLAALNELGDIVLFDIAEGTPQGKA
                     LDLAESAPIEGFDAALAGSNSYESIAGADVVIVTAGIPRKPGMSRDDLLGINLKVMES
                     VSTGIKKYAPDAFVICITNPLDAMVWALQKSSKLPPNKVVGMAGVLDSARFRYFIAEE
                     LKVSVEDVTAFVLGGHGDDMVPSLRYSTVAGIPLPDIVKMGWTTQERLDAIVSRTRKG
                     GGEIVALLKTGSAFYAPAASAIAMADSYLKDKRRVFPCAVKLNGEYGVKDLYVGVPVV
                     VGANGVEKIVEVSLDSSESQMFEKSVGSVRELVEACKSFNPTLAP"
     gene            2175..3371
                     /gene="sucC"
                     /locus_tag="VNY06_01230"
     CDS             2175..3371
                     /gene="sucC"
                     /locus_tag="VNY06_01230"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019199374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HWX13496.1"
                     /translation="MNVHEYQAKAVLKEFGVPVPRGIAVLQAADASPAAKELGGPIWV
                     VKAQIQAGGRGKGKFKEPEAGTGGGVRIAKSVPEVESFAAQMLGRTLVTVQTGTAGKE
                     VNRIYLEDGASIVKEFYLSVLVDRLASRIAFVVSTAGGMNIEEVARLAPDQIVSFNVD
                     PATGIMPHYGRTVASALGLTGSLAEQAENLTMQLYAAFVAKDMALLEINPLIVSAEGE
                     LKCLDAKISFDDNALYRHPEIAALRDETEEDAKEINASKHDLSYIALSGTIGCMVNGA
                     GLAMATMDIIKLYGAEPANFLDVGGSATKDKVAEAFKIITADHQVKGILVNIFGGIMK
                     CDVVAEGIIAAVKETGLSVPLVVRLAGTNADLGKKFIAESGLNVIPAGDLDDAAQKIV
                     AAAGRV"
     gene            3376..>3987
                     /locus_tag="VNY06_01235"
     CDS             3376..>3987
                     /locus_tag="VNY06_01235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006609294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HWX13497.1"
                     /translation="MPILADKETKVICQGFTGKNGTYHSEQAIFYGTKLVGGVSPGKG
                     GATHLGLPVYNSVAEARTATGADASVIYVPPSGAADAICEAIAAELPLIVCITEGIPV
                     ADMVKVKRALCGTKSRLIGPNCPGVVTAGQCKIGIMPGDIFKPGSVGIVSRSGTLTYE
                     AVFQTTIEGLGQTTAVGIGGDPVKGTEFIEILELFLADDLTKSI"
BASE COUNT          917 a         1087 c         1124 g          859 t
ORIGIN      
        1 tcgttaaaga aaacatgtgc cgctgtggtc acaaggttat tggtgactgt gagctggccc
       61 gcgccatgcg cgtcgccgca ttcgataaga cctgatccgg cgaaacgggt acgaagctct
      121 gctggatcta gtttttgctc cgccgcgaat tcctcgacgc tgcggcggct gtttttgcca
      181 aaaacgacga ccggaatggc cttgagcgct tcgccactcg gcaggttgtg tgaaaagagc
      241 ggcgctgccg tagcttcaaa agcaatccca agcaggaaaa tgcaaatggc tgcagcgtgg
      301 agccaaccct tttgaaacat tgcgctttct cctatcgatt cggccagcca tcagcaatag
      361 ggcagaccga aacagttcat caagctcgac cgcgatcgta tttagccatt gcgaaaacaa
      421 aaaggcagaa tccgaactga ggtgcccgca tttggttaac catgtgaatg ctgcttcctt
      481 ccgtctcggg agcacaaaaa ccgcaggccg cacggtggac cctaaagctc cacccattta
      541 gccacttaag ctcaagagct ggcttttcca atgaggcgag aatacgacaa aagccttgcg
      601 tgagccgtgg cgccaccccg gctcagcaat cacttaaatg tgccgaacgg gctaatccca
      661 gtttgtccca ggcgccaatt tacccctatt gtcgactctg ttagtataca atttctgggc
      721 agcgagcgat ggtaaagccc cgagttgcac atttcaccgc cgcaatgata acgtcgctgg
      781 gaaccggaat agcttcccac cgctcctgat tgtccgagcg tgtggccgca ggaggcagcg
      841 aaggacgaat agcacatggc gcgcaacaaa atcgcattga tcggagctgg tcagatcggg
      901 ggcactttag ctcaccttgc ggcgctcaat gagcttgggg atatcgtgct ctttgacatt
      961 gccgagggga caccgcaagg caaagcgctc gatcttgccg aatcggcacc gatcgaaggt
     1021 tttgatgcgg ccttggcagg aagcaatagc tatgaatcaa tagcaggagc cgatgttgtc
     1081 atcgtgacgg ccggcattcc gcgaaaaccc ggaatgagcc gggacgatct cctcggcatc
     1141 aacctcaagg tcatggaatc ggtcagcacc ggcatcaaga aatatgcgcc agacgccttt
     1201 gtcatctgca ttaccaaccc tctcgacgcc atggtttggg cattgcaaaa gtcttcgaaa
     1261 ctcccgccga acaaggtcgt cggcatggcc ggcgttctcg attcggcaag gttccgctat
     1321 ttcatcgccg aggaattgaa ggtctcggtc gaagatgtca ccgccttcgt tctcggcggc
     1381 catggtgacg acatggtccc ctccttgcgc tactccactg ttgcgggaat acctttgccg
     1441 gacatcgtca agatgggatg gacaacccag gaacggctcg atgcaattgt ttcgcgcacc
     1501 cgcaagggag gcggcgaaat tgtcgcgttg ctcaagaccg ggtcggcctt ttatgctccc
     1561 gccgcgtcgg cgatcgcaat ggccgactct tatctcaaag acaagcggcg ggtgtttccc
     1621 tgcgccgtga agctcaacgg ggaatatggg gtcaaagacc tatatgttgg ggtcccggtg
     1681 gtcgtcggag ccaatggtgt cgaaaaaatt gtagaggttt ccctcgattc atccgaaagt
     1741 cagatgtttg agaaatcagt cggctcagtg cgagaattgg tcgaagcctg caagtctttc
     1801 aatcccacgc ttgcacccta aattgttgcc gcagacggct catcggtcgg gaggattttg
     1861 gtcgcgctgg tcgtcactca tagtaactcc gcgccatcaa ttagggacaa aatacctaat
     1921 actcaattag ttacacagtt ttgctaaggt ttccaggtaa tattcgccgc ggtttcaatc
     1981 cgcctcctgc acccccgcgc gccacctagc cgcgcacacc gttgagttgg cgcagaaagt
     2041 gctagagcaa gacgacggtt cttccaatcg tccttttgct caagttcttg gtttttctcg
     2101 cgtctcagga ggccaaaccg gcttccgctt ttgctggaca cgcctcatat gaagcgcgaa
     2161 gcatgagact ttagatgaat gtccacgagt atcaggccaa ggccgtcctc aaggaatttg
     2221 gagttcctgt tcctcgaggg atcgcggtgc tgcaggccgc cgacgcgtcc cccgccgcga
     2281 aggagctcgg cggtccaatt tgggtcgtga aggcgcaaat tcaagcgggt gggcgtggca
     2341 agggcaagtt caaggaacca gaagccggta ctggcggagg cgtcagaatc gccaaatcgg
     2401 tgcccgaggt agagagcttt gcggctcaga tgctgggcag aacgctggtg acagtgcaaa
     2461 ccggcacggc aggcaaagag gtcaaccgga tttatctcga agacggagca agcatcgtaa
     2521 aggaattcta tctgtccgtt cttgtcgacc gcttggcaag ccgcatcgcg tttgttgtct
     2581 cgaccgccgg cggtatgaac attgaggaag tggcccgcct tgcgcccgac cagatcgtaa
     2641 gctttaatgt tgatccggcg acgggaatta tgccgcatta tggccggacg gttgcaagtg
     2701 ccctgggcct cacgggcagc ctcgctgaac aggcagaaaa tctcactatg caactctatg
     2761 cagcgttcgt cgccaaggac atggcgttgc tggagattaa tccgctaatt gtttcagctg
     2821 agggtgagct caagtgcctc gatgcaaaaa tctcgtttga tgacaacgcg ctataccggc
     2881 atcctgagat tgctgccctg cgcgacgaga ccgaagaaga cgcgaaggaa atcaacgcgt
     2941 cgaagcatga cctcagttac atcgcgcttt cgggaacgat cggctgcatg gtcaatggcg
     3001 cgggtctcgc tatggcaact atggacatca tcaagcttta cggcgccgaa cctgcaaact
     3061 tcctcgatgt tggcggcagc gctaccaagg ataaggtagc ggaagccttt aaaatcatta
     3121 ctgccgacca tcaggtcaaa ggcatccttg tgaatatctt cggcggtatc atgaagtgcg
     3181 atgtcgtcgc cgagggtatc atagccgccg tcaaggagac gggtctctcc gttccgctcg
     3241 tcgtccggct cgcaggcaca aacgccgact tagggaaaaa attcatcgcc gaatcgggtc
     3301 ttaacgtcat tcccgcgggc gatctcgacg atgccgcgca aaaaatcgtt gcagccgccg
     3361 gaagagtttg aagctatgcc aattcttgcc gacaaggaaa cgaaagtcat ttgccaagga
     3421 tttaccggta aaaacggaac ctaccattcc gaacaggcaa ttttctacgg cacaaagttg
     3481 gttggcggcg tgtcgcccgg taaaggcggc gcgacgcatc tcggcctgcc tgtctacaat
     3541 tcggtcgcgg aagcacgcac cgcaacgggc gcagatgcca gcgtcattta tgtcccgccg
     3601 tctggagccg cggatgcgat ctgcgaggcg atcgcggcag aattaccgct cattgtctgc
     3661 atcaccgaag gcatcccggt cgcggacatg gtcaaggtca aacgcgcgct gtgcgggaca
     3721 aaatcccgtc tgatcggacc aaactgtccg ggtgtggtga ccgccgggca atgcaaaatc
     3781 ggcattatgc cgggcgatat attcaagccc ggttcggttg gaattgtgtc gcgctccggc
     3841 accttgacct atgaggcggt atttcagaca acgatcgaag gacttggcca gacgaccgcg
     3901 gttggtatcg ggggtgaccc agtcaagggt acagaattca ttgagatatt ggaattgttc
     3961 ctggccgacg atcttaccaa atcgatt
//
LOCUS       DATMPU010000054         5258 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_660801,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000054 DATMPU010000000
VERSION     DATMPU010000054.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 5258)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5258)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5258
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_660801"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1687..2508)
                     /locus_tag="VNY06_01240"
     CDS             complement(1687..2508)
                     /locus_tag="VNY06_01240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX13498.1"
                     /translation="MHKLHCISNACGTQRVDAQAASNGAIPDELVPCPRPGRGSQRSG
                     GNTDYGAGQPDNQYAQKAKNSFFSASNFFRNGVFSKAAEGFALAEGYYREAGDTQNAA
                     NARRNKNLSTCNDRLNYLIVNDPEALRILLGEGKELRNPVVIDGITTFEPMLGACSEF
                     PPEADTIRRKLAALEEEKHATSTPEQSTDNGKSPYRPVDVTQCISSWRKPMNLGYIDW
                     GVKNSCDVAISFDIDDCDMNEDLQAVCTVKTLTVAAHSDYDGSNYHQPANARNYR"
     gene            complement(2558..2791)
                     /locus_tag="VNY06_01245"
     CDS             complement(2558..2791)
                     /locus_tag="VNY06_01245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX13499.1"
                     /translation="MRQHSNLAKDTAEKVVKDVRRATRKQYSAEEKIIRAHAIGTHRV
                     AGNRALWAGQPGARVLAAQSHDEAPAPQAMSVA"
     gene            complement(2887..3054)
                     /locus_tag="VNY06_01250"
     CDS             complement(2887..3054)
                     /locus_tag="VNY06_01250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX13500.1"
                     /translation="MVRFLPATLVLAATFAAVARPAGAVSMCGDRNFGGTIVHECYDA
                     ADSYVTFTLDI"
     gene            4791..5000
                     /locus_tag="VNY06_01255"
     CDS             4791..5000
                     /locus_tag="VNY06_01255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX13501.1"
                     /translation="MSLKCAHRWRVQRRRVRIIVPDQGLSDMNDVLQQGGGWRVQSDC
                     QSSDLRPGTDTAWALSASITRTRIW"
     gene            complement(4974..>5258)
                     /locus_tag="VNY06_01260"
     CDS             complement(4974..>5258)
                     /locus_tag="VNY06_01260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008140202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HWX13502.1"
                     /translation="VEVCPVDCFYEGENMLVIHPDECIDCSVCVPECPVDAIQPDTDP
                     GLEKWLSLNAKYAKIWPKITVKKALPPDSREWEGKPDKLQYFSPNPGAGD"
BASE COUNT         1158 a         1489 c         1456 g         1155 t
ORIGIN      
        1 gcacccagcg gcaattctgc attcgtaagc agcaggaggc tgccttgtgt cctcgatgcg
       61 atcataccga ccgacaaacg cacggaggaa tgctacaggc ttcgccacga gggaggcgag
      121 tgctcttgtt caccgaattg atcggccgct ggcgtgagag aaccgatgaa tgcccggaca
      181 ccaaaagacg ctctgccgac ttaactttgc cacttgccca tctattttgc gggcgcaacc
      241 aatccgctgc cggtagaccc cccggagcgg tgaccaccga gaccggtgat gtcacttggt
      301 tgatggggtg gcgccgggcg gcttggagcg ccttgaccat aaggaacaca gattgagttc
      361 tcgccgtagc cttggatcat gcggtacatg cccgcagggc aatcctgcgc ccacgctgga
      421 gcgacacaca acatcgcggc tatggacgca aatgctaacg gagatgtctt caaggagtcc
      481 tcctatttgg tgggccccgg cggccagcac tggctagttg caaatgcccg cgcggcagat
      541 gcacgagtag tttcctgccg cattttgcag actcattcct gtaatggaag gaatctgaaa
      601 cgtttgcggt ggtcgagtgt gataggactg cgatgggcag acgaggcgag gctgacccca
      661 cgatgctgag ctgcccgtgg acaccgcagc atcgacgaag ccaacagaga cattgaaacc
      721 gtccggacag gagctactca cctgatagat cggcatgcca gttgggtcgt attctccact
      781 cggagtaacg cttatgcatc cgcaatcatt tggtccgggc gctcgattcc cgagcgtgca
      841 gcccccaccg gttttcaaat aggcttcaac ccatggtcgg cattgccgaa gcaaattttg
      901 gacaggcgga ttgtgggctt gggcgctgac gacggcttca aatgccgatt ctcccatttg
      961 ccgatgcaaa ttgtaggtat tggtcgctcc ccgatcggca aatacccagt ccacatttat
     1021 ctttgctttt gcctggagtg cagggtcgca cggcccggac cctcgacact gatctatgga
     1081 ttgatcgcaa ttgcaagctt gaacagcatt tcgccggcag ctggctgcgt tctgtagccc
     1141 ggtttgctga acgcaggttc ctgcgtgcag cgttatttcc tcattgcaac taagagccaa
     1201 cagcggctcg gacatcaatg acacgagcag gaagatgcca acacgcgcgc cgctcaccat
     1261 gccgaatggg cggtggccat gcgcaatcca tattgccgtg ggtatcactg caagctgtta
     1321 caaaggggcg gcattgggca gccgccggcg gtcattgctt ggcagtaaac ctgaacgcac
     1381 tctttaaatg agccagcttg gccgctggat cctccaccta tattttgcca gttgcggtaa
     1441 caatttgacg cgttcgcaag cgcctgttga cacattgcag cggtgccttg agcgaggtgt
     1501 gttgcgttcg taggggcgcg aaaaaagtca gatgcaaggg tgatttgcgt gccggttaac
     1561 aaaaatgcga acgctccagc tgccacgtaa ttcacgatta taccctcccc atgagtaaat
     1621 catggagtgc aaccaggact gcgcgcgaaa tcggcttaga cagcttcagg ccctaattct
     1681 tcgtcctcac cgatagttcc gcgcatttgc tggctgatga tagttgctac catcgtagtc
     1741 ggagtgggcg gcaactgtca gcgtcttaac tgtgcagacg gcctgcaaat cctcgttcat
     1801 atcacagtcg tcgatgtcga aggaaatcgc aacatcgcaa ctgtttttga cgccccaatc
     1861 aatgtagccc aaattcattg gtttcctcca actggagata cattgtgtga catcaaccgg
     1921 acgatagggg gacttaccgt tatccgtgct ttgttccggt gtagacgtcg cgtgtttttc
     1981 ttcttccagt gcggctagtt tgcgacgtat cgtatccgct tcaggtggga attcggagca
     2041 cgcgccaagc attggctcaa acgtcgttat tccgtcaata acgaccggat tccgtaattc
     2101 cttgccttcg cccaacagaa ttcgaagagc ttcagggtcg ttaacgataa gatagttgag
     2161 gcggtcgttg caagtagaca agtttttatt tcggcgcgca tttgcagcat tctgagtgtc
     2221 gccggcttcg cggtaataac cctctgccaa agcaaaaccc tcggcggctt tgctaaagac
     2281 cccattccgg aagaagttac ttgccgaaaa aaaactattc ttggcctttt gcgcatactg
     2341 attatcgggc tggcccgccc cgtaatccgt attgccgcct gatcgttgtg atccgcgccc
     2401 cggccggggg cagggcacga gttcgtccgg aatggcgccg ttgctggcgg cctgcgcgtc
     2461 aactctttgg gtcccacagg cgttgctgat gcaatgcagc ttatgcacgt tggaatttcc
     2521 tcccaaacgc atcgaccgta accatacaat gacgcaatca cgcgacactc atggcctgcg
     2581 gagcaggcgc ttcgtcatgc gattgagctg ccagaacgcg agccccaggc tgaccagccc
     2641 aaagagcccg attgccggcc acgcgatgcg tgccgatagc gtgcgcccgg atgatctttt
     2701 cctccgccga atactgcttg cgtgtcgccc ggcggacgtc cttgacgacc ttctcggccg
     2761 tatccttcgc caaattggaa tgctgtctca tcttcgctcc tgtgaacgct acgatgagcc
     2821 agaaacactc ccttcctcaa gctgcccaat ttggcccacg agtgctgacg gtggacagcg
     2881 gttcagtcaa atgtcgagcg taaacgtcac gtagctgtcg gcggcatcgt agcattcgtg
     2941 cacaattgtc cccccgaagt tgcggtcacc gcacatgcta acggcgcccg ccggccgggc
     3001 aactgctgca aaggttgccg caagaacgag ggtcgccggc agaaatctga ccacgaagtt
     3061 cgtgcgcgct gattgagctg ttcccgtcat tgacgccgat ttcagccctt gaagcaagga
     3121 gagaaagtgc tctaacattt gttccgcttc gtcgagatcg tggcataaac agctgccaaa
     3181 cgagattgga tatcgaagtc ttatagcgct tcgaccaggg aaacaagcgc ctccggcgtt
     3241 ctctgccgac gctgttcttg gcatgcaact cgccgcaaat tatgggaaga aaagaataag
     3301 atcggaacat ctgtatcagc tctatgcata cttgaagaat tgcaagtcgc aaggtgagct
     3361 gccagagggt attttgcttt atccaacgac atcgcagtct ctcgaccttc ggtttgacat
     3421 gggtgggaat aagttgcgca tgaagacact gcaattaaat cagccatggc aaaaaattca
     3481 cgcggaattg tccgagctgc ttatcgcgca aaagtcggac aatttgcctt tttcatcttt
     3541 cgtcgcgtag gccttagctg ttgcgggtct gcgaggccta gttcgtgaag ccgttaagaa
     3601 cataatgagc gcgttcgctc gggtaccaac gcgtgcgata agcatttagc cggttggaaa
     3661 acatgttccc gcccttgcca gaaacgtatg caaggaaatc gggagagcga ctgatttggt
     3721 ccagcacgcc tccccaagat ttaggcgggc ttgaataagc caatctacct cgtcagtcct
     3781 ccacttgagg ggcctgctac ctcccaaatg gcccgcattg ctcttggcga gtccgccatg
     3841 gccgcagaca ttgcgaggag gcccggtccc agatcatcca gtgtgaattc ctcggggcaa
     3901 cgaccacgga gatctaggcg atcgcacgcg agcgcgccgc gccccgctag aagatcgtga
     3961 aatgagcgtc tgcagcacaa agacccgaac cgcggcgctg agcgtgcgta ctcggcgata
     4021 tcctgggcgg gctctctggt tcacactgct aatgcgctgt gagccctttc cgacgccgca
     4081 ccaagcgatg aaagctagcg acgatccaaa ttacaaagcg tccgatgccc caagaaagga
     4141 cccacaacag aacgagtgga atcaacagga agccccaccc tgctacccca agtatcctgt
     4201 cgccgctgcc gaacatcgcg ctaagcactc ccggaagacg gacaccgtcg cgtgcgtagg
     4261 catgattaca cgcatccatt tctgcaatgt atctatacat gtcaaaccga ggcgagtcgc
     4321 ttgacccgta ttgttggtaa gcggaatcag agcagtcgtg caaagcctgt gcggggccac
     4381 gatttgcttc tgtggcaatc aataaaggcg agacaatcgc ccagcaaatg gcaatcacca
     4441 cgaataagcg attccaaggg ttcatgtatt tctgggcctc gccattacta agcgcggatc
     4501 gcttactgct gccactggaa gcgcgttcgc ggcaggctcg tcagtggcga ggatgtagag
     4561 tctacagtgg cggctcgaca gctggggctc agacctagtt cggctccctg tgatcagcga
     4621 ccttcgcgat gagcactgat gatcgatcaa gtcggcgaca gaaacagact gaccatcggc
     4681 gaggatgtag agactacact ggcggcgcgg cagctgagcc tggccccggc gtgggcccca
     4741 acgtcggttg cctgacaatc ctcggggagg cgggcaagcc aagtatgatc atgagcctta
     4801 agtgcgcgca ccgctggcgg gttcagcgcc gccgggtgcg gatcattgtg cccgatcagg
     4861 gcctgtcgga tatgaatgac gtgctgcaac agggcggcgg gtggcgtgtt caatcggatt
     4921 gtcagtcctc cgatctcagg cccggcaccg atacggcatg ggcgctctcc gcctcaatca
     4981 cccgcaccag gatttggtga gaaatattga agtttatccg gcttcccctc ccactcccta
     5041 gagtcgggcg gcagcgcttt tttcaccgta atctttggcc agattttcgc gtatttcgca
     5101 ttcagtgaca gccatttctc aagtcccggg tccgtgtcgg gttgtatagc gtcgacagga
     5161 cattccggca cacaaacact gcagtcgatg cattcgtccg ggtggatcac gagcatattc
     5221 tcgccctcgt agaagcaatc cacggggcag acctcgac
//
LOCUS       DATMPU010000055         2427 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_3140783,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000055 DATMPU010000000
VERSION     DATMPU010000055.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 2427)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2427)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2427
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3140783"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1..382
                     /locus_tag="VNY06_01265"
                     /pseudo
     CDS             1..382
                     /locus_tag="VNY06_01265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012606126.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
     gene            complement(492..1085)
                     /locus_tag="VNY06_01270"
     CDS             complement(492..1085)
                     /locus_tag="VNY06_01270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012590638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1134 domain-containing protein"
                     /protein_id="HWX13503.1"
                     /translation="MHTRREMTVMFAAFAAAVPVIRAKSALADTPAPSGRPAEFSPSE
                     IIDSGHHFFGSISRGLAEVVEAATKRWGKPNGYVLGQEAGGAFIGGLRYGEGTMYTRN
                     AGDFKVFWEGPSLGFDAGADGDRTMMLVYNLPSTDAIFRRYGGVDGSAYLVGGFGMTA
                     LVNDGVFVVPIRSGIGARLGFNVSYLKFTPSSTWNPF"
     gene            complement(1246..2103)
                     /gene="cysQ"
                     /locus_tag="VNY06_01275"
     CDS             complement(1246..2103)
                     /gene="cysQ"
                     /locus_tag="VNY06_01275"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012590639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HWX13504.1"
                     /translation="MYKTKKDIDTCKVKAFDADAVAEIFAEIALDAGVAVMEVYAGDS
                     RARRKTDGSPVSDADEAAEAIILERLAACLPDLPVLAEEAASRGGTRVTGPAFILVDP
                     VDGTREFLSRNREFTINIAFISDGLPCAGVVFAPALGKLWIGGSGASACDAAPGESLP
                     PAARRRVIIARPAPKKGLTALVSRSHTDPATEAFLAELPIKERREAGSSLKFCAIAEG
                     KADVYPRFGPTMEWDTAAGDAVLRAAGGVVVDAHGRPLRYGKSEAHFGNGPFVAWGDP
                     KAVAVGGSQ"
BASE COUNT          538 a          749 c          619 g          521 t
ORIGIN      
        1 ctcatcgcga tatcggtgcg gccttggtct tgccttatgc caacgcccaa gcgatgaatc
       61 tgcatctcaa ggagatcagc cgccaggtga cgcaaggcag ccatgccgtt ttgctcctcg
      121 atggagccgg atggcatcag acgggcggca agctgcgcgt cccaggcaac atcagcctac
      181 ttccctgccg ccctattcgc ccgagttgaa tccggtcgaa aacatctggc agttcctgcg
      241 ccaaaaccac ctcagcaacc gcattttcaa aaactatacc gacatccttg atgcctgttg
      301 cgccgcatgg aacgccctcg tcgaggagcc aaaacgcatt gcctcaatcg ccacacgaga
      361 ctgggcgtcg gtcattccct aatgccgttg gtcacaacac tcatgcacct tgggttgcct
      421 ttggcattga gcgcgcgtgc tcgagccatc gtccgaaaat atctggcaat cgtttgatgt
      481 aggtgttcgt cctaaaatgg attccacgtc gagcttggcg tgaatttcag atagctgaca
      541 ttgaacccaa gccgtgcgcc gatgcccgag cggattggaa caacaaacac accatcgtta
      601 accagcgctg tcatgccaaa accgccgacg agatacgccg agccatccac gccgccgtag
      661 cggcgaaata tagcatccgt cgaaggcaga ttataaacga gcatcatcgt gcggtcgcca
      721 tcggctccgg catcgaaacc aagagagggt ccttcccaaa ataccttaaa atccccggcg
      781 ttgcgcgtgt acatcgtgcc ttcgccgtag cgcagaccgc caataaacgc gccacctgcc
      841 tcctgaccca agacgtaacc attgggtttg ccccaacgct tggtggcagc ctcgacaacc
      901 tcggcaagcc cccttgagat cgacccaaag aagtgatggc cgctgtcaat gatctcactg
      961 ggagagaatt cggcgggccg cccgcttggc gcgggcgtat ccgctagtgc acttttcgcc
     1021 cggatgaccg ggacggcggc ggcaaaagcc gcgaacatca ctgtcatttc ccttcgcgta
     1081 tgcatgatag tcctccgctc aaacttccgc gactgagacc ttgtgactat accgtgcgga
     1141 tattgtgcag acttttgatc taatagcgtt aacgaaggat tgacgggcgc ttatcgcagc
     1201 cttattatgg cgaatatttg cacgttttct tgaccgtgct tgcgattatt gcgaccctcc
     1261 aactgcaacc gccttcggat caccccaagc aacaaagggc ccattcccga aatgcgcctc
     1321 ggatttccca taacgcaatg ggcgtccatg cgcatcaaca acaacgccgc ccgcagcgcg
     1381 caacaccgcg tcgccagctg cggtgtccca ttccatggtt gggccgaagc gcggatagac
     1441 gtcggccttt ccttctgcga tagcacagaa ttttagcgag gagccagcct cacgccgctc
     1501 ctttatggga agttctgcca gaaatgcctc ggtcgcggga tcagtgtgag atcggctgac
     1561 cagcgccgtc aatcctttct taggtgccgg ccgcgcaata atgacccgac gcctggccgc
     1621 cggcggcaga gactcacccg gcgcggcatc gcaagcactg gcaccgctac cgccgatcca
     1681 gagcttccca agcgctggag caaaaaccac tcctgcacag ggcaaaccat cactaatgaa
     1741 cgcaatattg atcgtgaact cgcggttgcg gctcaaaaat tcgcgcgtcc catcgacagg
     1801 atcaaccaaa atgaaggccg gtccagtaac cctcgtcccg cctctcgagg ctgcttcctc
     1861 agcaagcacc ggcagatccg gcaggcacgc cgctaggcgc tctaagatga tggcttcagc
     1921 tgcttcgtca gcatccgaaa cggggctgcc atccgtcttg cgcctggcgc ggctgtcacc
     1981 cgcataaacc tccatcaccg cgacacctgc atcgagcgcg atctcagcga aaatttctgc
     2041 cacggcatcg gcgtcgaacg cctttacttt gcaagtatct atgtctttct tggttttata
     2101 catgaggttt tctgaaacag acgtttgtca catgttcatt gcacaatttg cacttgctga
     2161 acgagagaag ttgcgagata cgataatttg ttaacctctg attcgcagac tagaagatag
     2221 atctttgacg tctcggcgcg ccacggattc gattcggctt gccgttacgt attggtaccg
     2281 tacgcgcgct ggcgacagct tcgggcattg cgcgcgatgc aactttgtag cgcgatgcat
     2341 agacgcaaag cgaagggata gttcatgaca gtgtttgctt tcgactccat cgatctcgcc
     2401 gcgcttttat gctctcgcgt ctgccat
//
LOCUS       DATMPU010000056         3334 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_661542,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000056 DATMPU010000000
VERSION     DATMPU010000056.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 3334)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3334)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3334
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_661542"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..396)
                     /locus_tag="VNY06_01280"
     CDS             complement(<1..396)
                     /locus_tag="VNY06_01280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX13505.1"
                     /translation="MGNCFVRVTSKQRRQDSNLPALAAGRNNSGGASRINAFGDAQSF
                     ARGCDGKLGALVYAVFPLAARGHNNCPAKTFAAKRAVASAISEGESSRRKSVPCTALH
                     DDLLLVWRSLTTLSPLTVSANLGKAGWSWF"
     gene            479..1552
                     /locus_tag="VNY06_01285"
     CDS             479..1552
                     /locus_tag="VNY06_01285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013946038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HWX13506.1"
                     /translation="MIDLSKPFPRQPAAVQWMILVFLSVIFVGTLETLRFPAALLIGP
                     MGAAILATINGMTLRLPPWVFLGAQGIVGCMIAHSGSHPIDSVMFTRWPLLLATVLAI
                     IIASTILGWLLARRGLLPGAVAVWGSTPGAANVMMLMADAHGADIRLVAFMQYLRLLI
                     VTLIASLVARVFVSQGPIRAIIDWFPAISWSAFIATLALVTIGVVAGRALKIPAGPLL
                     LPMALAAALQGLSSLQIELPPWLLALTFASLGWHIGLGFSPATLTHVARAFPKVLALI
                     LVQILICGGLGFLLTSFAGVDALSAYLATSPGGADSIAIIAASANVDLAFVMAMQTFR
                     LVLVILISPALCRVVVRHIDRTG"
     gene            complement(1639..2517)
                     /locus_tag="VNY06_01290"
     CDS             complement(1639..2517)
                     /locus_tag="VNY06_01290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008875107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HWX13507.1"
                     /translation="MSEIDAIRALLSSKPRPVGWQERRKRLDEVGSVWPVAEDIRLET
                     IRLDGLPAEWSFAPGSDAARVLMFFHGGGYCSGSILSHRRMVTEAGRAAGSRTLAVGY
                     RLAPENPFPAALDDALASWRWLRSQNIAANHIAVGGDSAGAGLTVSLITRLREDGEEL
                     PGCAWLASPWTDLTVSGETLVSKDAVDPLIHKAYLMELADAYLPPGMDRKDPRVSPLY
                     TDLKNFPPVLIQVGAEETLLADATRFASAAGAANVPVTLEIWPHMIHAFALWNAHLEP
                     GRRALANAGSFIRKHL"
     gene            complement(2556..>3334)
                     /locus_tag="VNY06_01295"
     CDS             complement(2556..>3334)
                     /locus_tag="VNY06_01295"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HWX13508.1"
                     /translation="PHMILLRSLAFHASFYLLTTVLAIVGLPVLLLNRHRVQNYAKFW
                     TGSTVWLLEKICKTKLEWRGLENLPRGACIIAAKHQSALETQALTTKGADFSFILKRE
                     LMAIPVLGWYLKGAGQLGIDRAKRAQALNDLARQARGAIAQGRQLIIFPEGTRKPVGN
                     TPDYKPGVALLYSETKAVCIPVTLNTGLFWPRHSLVIRPGTVTIAFLEPIAPGLDRKT
                     FMQLLQSRIETETAKLVAEALAADPTLTSVSQKRQAAAGP"
BASE COUNT          626 a          987 c          964 g          757 t
ORIGIN      
        1 gaaccacgac caacccgcct ttccaagatt agcactgact gttaggggtg acaacgttgt
       61 caagcttcgc caaaccagca gcagatcatc gtgcaaggcc gtgcaaggga cgcttttcct
      121 gcgagaggat tccccctctg agatcgcgga agcgacggca cgctttgcag cgaaggtctt
      181 tgccgggcaa ttgttgtgac cacgagccgc cagtgggaac actgcgtaca ccagcgcgcc
      241 aagcttgccg tcacaacctc gcgcaaagga ttgcgcatcg ccgaacgcgt tgatcctgct
      301 tgccccgccg ctgttgttcc ttccggccgc caatgcgggc aggttagagt cttgtcgacg
      361 ctgcttagag gttacacgaa cgaagcaatt tcccatgggc tcatccctcc gccgcctcgt
      421 cccgtagggc ccgtcagaga accgcggtgt cccccgccag ccattgagtg tccgagctat
      481 gatcgacctt agcaagccat tcccgcgcca gccggccgcc gttcaatgga tgattttggt
      541 ctttctttcg gtaatatttg tcggaacact ggaaacactg cgctttccgg cggcgctgct
      601 tatcggcccg atgggggctg ctatccttgc gactatcaac ggcatgacgt tgcggctgcc
      661 tccctgggtt ttcctaggcg cgcaagggat tgttggctgc atgatcgccc acagtggcag
      721 ccatccgatt gactcggtaa tgttcacccg ctggccattg ctcctggcga cagtccttgc
      781 aatcatcatc gccagtacta tccttggatg gcttcttgcg cgtcggggcc tcctgccagg
      841 tgcggtggcc gtatggggtt caacccccgg cgccgccaac gtaatgatgc tcatggcgga
      901 cgcccatggc gccgatatcc gcctcgtcgc cttcatgcaa tatctgaggc tcctaatcgt
      961 tacccttatc gcctcgctcg tcgcgcgcgt cttcgtttcg caagggccga taagagcaat
     1021 aatcgactgg tttccggcga tatcgtggtc agcgttcatc gcaactttgg cgcttgttac
     1081 gatcggcgtt gtcgcgggac gcgccttaaa aattccagca ggaccgctat tgttgccaat
     1141 ggctctcgcc gccgcgctgc agggcctcag ctcactgcag atcgaattgc cgccctggct
     1201 attggcattg acctttgcat cgctcggctg gcacattggc ctcggttttt cgcctgccac
     1261 cctaacacac gtggcccgcg cctttccaaa ggttctggct ttgattctcg tgcagatttt
     1321 gatctgcgga gggctcggct tcttgctcac gagcttcgcg ggtgtcgatg cgctgagtgc
     1381 ctatttggcc acaagcccgg gcggagcgga ctcgattgcg atcattgccg cctcagccaa
     1441 tgtcgacctt gccttcgtca tggcgatgca gactttccgc ctcgtgctgg tcatcctcat
     1501 tagtcccgcg ctttgccgcg ttgtcgtccg gcacatagat cgcacgggct gatttttggt
     1561 tcatctccag tcaggattga catgctcttt cttatttgca cgtgcagttc ggcggatgtt
     1621 tttcgtggtt ctctcatatc agagatgttt acggatgaac gatccagcat ttgcgagcgc
     1681 tcgccggccg ggttcaagat gcgcgttcca gagggcgaag gcatgaatca tatgcggcca
     1741 gatttccaag gtcacgggca cattggcagc accggccgcg ctagcaaagc gcgtggcgtc
     1801 tgcgagcaag gtctcttctg ctccgacctg gatcagcacc ggcggaaagt tttttagatc
     1861 cgtataaagc ggtgaaaccc gcgggtcttt cctgtccatg ccgggtggaa gataagcatc
     1921 ggcgagttcc atcagatagg ccttgtgaat gagcggatcg acggcgtcct tgctgacgag
     1981 cgtctcccca gacacggtca aatccgtcca aggcgaagcg agccatgcgc aaccgggaag
     2041 ctcctcgcca tcctctcgca gccgggtgat cagcgacacg gtcagcccgg cccccgcact
     2101 gtccccgcca acggcgatat gattggccgc gatattttga cttcgcagcc accgccagga
     2161 ggccagcgcg tcgtcgagcg ccgcgggaaa aggattttct ggagccagcc gatagccgac
     2221 ggccagcgtc ctcgagcccg ccgcccgccc cgcttcggtg accatacgcc gatggcttaa
     2281 gatcgagccc gagcaatagc cgccaccgtg aaagaacatg agaacacgtg cggcgtcgct
     2341 gcctggcgcg aatgaccatt cggcggggag gccatcgagc cttattgttt caagccttat
     2401 gtcttcggcg acgggccaga cggacccgac ctcgtcgagg cgtttgcgcc gctcttgcca
     2461 tcccacgggc cggggcttgg aactcagtaa cgctcgaatc gcatcaatct cgctcacaat
     2521 ccattgcctt ctcttcccaa tggtattcgc aaaaattatg gtccggcggc agcctgtctt
     2581 ttttgcgaaa cggatgtgag tgtgggatcg gcggcgagcg cttcggcgac gagctttgct
     2641 gtttccgttt cgatccggct ttgcaaaagc tgcatgaaag tttttctatc aaggccgggc
     2701 gctatcggtt ccaaaaaagc aatcgtgacc gtgccaggtc taatgacaag actatggcgc
     2761 ggccagaaga ggccggtatt caaggtcacc ggaatgcaga cggctttggt ttccgaataa
     2821 agaagcgcaa cgcccggctt atagtccggg gtgttgccca cgggtttgcg tgtgccttcg
     2881 ggaaagatga taagctggcg gccttgcgcg atggctccgc gcgcttggcg tgcaagatcg
     2941 tttaaggctt gcgcgcgttt tgctcggtcg atgccgagct ggcccgcgcc tttaagatac
     3001 cagccaagga ccggaatggc catcagctcg cgtttgagaa tgaaggaaaa atccgcgcct
     3061 tttgtggtga gcgcttgggt ctcgagcgcc gattgatgtt ttgccgcgat gatgcaggcg
     3121 ccgcgcggca gattttcaag cccgcgccat tcgagtttgg ttttgcagat tttttcaagc
     3181 agccaaaccg tcgatccggt ccagaatttt gcgtaatttt ggaccctgtg ccggttcaac
     3241 aaaagaacgg gcaggccgac gatcgcgagc accgtcgtca gaagataaaa ggatgcatgg
     3301 aaggcaagcg aacggagcaa gatcatgtgg ggct
//
LOCUS       DATMPU010000057         3450 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_662836,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000057 DATMPU010000000
VERSION     DATMPU010000057.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 3450)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3450)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3450
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_662836"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            85..207
                     /locus_tag="VNY06_01300"
     CDS             85..207
                     /locus_tag="VNY06_01300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX13509.1"
                     /translation="MARDDFAKFPKAAYMTEIESWRVLAGDRIEFTIRRLPSAD"
     gene            350..754
                     /locus_tag="VNY06_01305"
     CDS             350..754
                     /locus_tag="VNY06_01305"
                     /inference="COORDINATES: protein motif:HMM:NF018527.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1236 domain-containing protein"
                     /protein_id="HWX13510.1"
                     /translation="MGILQKFGAAIALIGSFALPLTAQAQGAGGGAEGAGQIAGPSRQ
                     APGAASSGGPSLIGADQRTHFRKYAMRKHRATPYSFREPVAAGTILPPVGIELYEIPP
                     KFKVPAEYRYAVVNNLVLLVDPVTHQIVEVIE"
     gene            complement(815..2035)
                     /gene="mutY"
                     /locus_tag="VNY06_01310"
     CDS             complement(815..2035)
                     /gene="mutY"
                     /locus_tag="VNY06_01310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012590619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HWX13511.1"
                     /translation="MAFQSHRDRRADIANALLAWYDCHRRDLPWRAPPGECTDPYKVW
                     LSEIMLQQTTVAAVKPYFEAFLSRWPNVAHLSQASRDEVLRQWAGLGYYSRAHNLHAC
                     AKIVAEKLDGNFPVTETALLRLPGVGPYTAAAIAAIAFGQPAAAVDGNIERVVARLFA
                     IKTPLPGAKTSIRAKARQLVPRDRPGDFTQAMMDLGAILCTPRNPCCNVCRLRNFCLG
                     HASGDVVLPRKAARPKRPIRQGAIFIVEREDGAVLVRSRPPKGLLGGMAEFPGTAWER
                     HFDARAAIRHAPVEAVYRKLEHKVAHSFTHFALTLEIYVAEVPGRQRAPAGCRFVPRR
                     ELDREAFPSVMRKVIEAVRFDEARGQGSVKPLKVPSTEQRQEGELAQAGSPPRQRIKR
                     VRHKQTNAVCLALP"
     gene            2103..2570
                     /locus_tag="VNY06_01315"
     CDS             2103..2570
                     /locus_tag="VNY06_01315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012590620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HWX13512.1"
                     /translation="MSAKPIPEGHEAAIPYLAVRNASDALTFYKNAFGAVETLCIAHG
                     AKIGHAELLIGKARVMLCDEFPDHEVLSPQTIGGTPIMIHLYVDDVDAFTSRAIDAGL
                     KVLRPVKDQFYGDRGGKFEDPFGHRWWIATHKEDLTPEELKTRAAALCSNGPA"
     gene            2654..3145
                     /locus_tag="VNY06_01320"
     CDS             2654..3145
                     /locus_tag="VNY06_01320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012590621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DciA family protein"
                     /protein_id="HWX13513.1"
                     /translation="MRNRPGAKIPWSRPLGDLVQSAIDPVLTRRGFGLTALVLYWDDI
                     VGERLAAVSRPVRVQWPARQRDPAVPAALVVRVETGCALELQHLAPVILERVNAHFGW
                     RSISRLVLMQGPVAAPPAARRVAQPLNKAAEAAAAELVGGDLDHSLRLALVRLGARVL
                     AGP"
BASE COUNT          680 a          975 c         1058 g          737 t
ORIGIN      
        1 aaacccggcc gggctcgcac gccgtctcgc caatagggcg gcgcgccgcg cggcagctcc
       61 caagcacgca acgaggcgcg tatcatggcc cgcgacgatt tcgccaaatt tccgaaagct
      121 gcctatatga cggaaattga atcctggcgt gtcctcgcgg gcgatcgcat cgaattcacc
      181 atccgccgcc tgccaagcgc tgattagcta gcaggagttc cgtctttcta gcttcgttcg
      241 tcagcttgtg ggggccattt aagaatgtgt gagacggaag tgccccgatt cgacattctt
      301 aattgagctg caatcgtttg ctcaattcgt gaacatcggg aggtatgtta tgggaatcct
      361 gcagaaattt ggtgcagcta tagcgctcat cggatcgttc gctctgcccc tgactgcgca
      421 agcgcaggga gctggcggcg gagctgaagg ggctggccag atagcgggac catctcgtca
      481 ggctcctggg gccgcttcga gcggcggtcc aagtcttata ggcgccgatc agcgtaccca
      541 tttccgaaaa tatgcgatgc gtaaacacag ggctacgccc tacagttttc gcgaaccggt
      601 tgcggcgggt acgatcctgc cgcccgtagg aatagagctt tatgaaatcc caccaaaatt
      661 caaagtccca gcggaatatc gatatgcggt cgtgaataat ttggtacttc tcgttgatcc
      721 ggtaactcac caaatcgtcg aagtgattga gtgaagccgc cgcccgcaga ccgtgtgttc
      781 cccgagcacg atcccaaaaa ggtgattggc catttcaggg aagcgcgagg caaacggcgt
      841 tagtctgctt gtgccgaacg cgcttgatcc tctggcgcgg cggggatccg gcctgagcaa
      901 gctcaccttc ttgccgctgc tccgttgagg gcaccttgag gggtttcacc gagccttgcc
      961 cgcgggcttc atcaaaccgt acggcttcga tgaccttgcg catcacactc ggaaaggcct
     1021 ccctgtcgag ttcgcggcgt ggcacgaacc ggcagcccgc gggcgcgcgt tgcctaccgg
     1081 gaacttcagc cacatagatt tcaagcgtca aagcaaaatg agtgaagctg tgggcgactt
     1141 tatgctcgag cttccggtaa actgcctcaa ccggcgcgtg tctaattgct gcgcgtgcat
     1201 caaaatggcg ttcccacgcg gttccgggaa attcggccat gccgcctagg agaccctttg
     1261 ggggccggga gcggacaagg accgcgccgt cctctcgttc gacgataaag atggcaccct
     1321 ggcgaattgg ccgctttggc cgcgctgcct tgcgaggcag cacaacgtcg cccgaggcat
     1381 ggccgagaca aaaatttcgc aagcgacaaa cgttgcagca agggtttctc ggcgtgcata
     1441 ggattgcgcc gaggtccatc atcgcttggg taaagtcgcc cggccgatcg cgtggcacga
     1501 gttgccttgc ctttgcccgg atggaggtct ttgcccctgg cagtggcgtc ttgattgcga
     1561 aaagccgtgc aacaacccgc tcgatattgc cgtcgaccgc agccgctggc tgtccaaagg
     1621 caatcgccgc gatcgccgcc gctgtgtaag ggccgacacc aggcaaacga agtaatgcgg
     1681 tttcggtcac tgggaagttg ccgtcgagct tttctgcgac aattttcgcg caagcatgga
     1741 ggttgtgcgc gcgcgaataa tagccaagcc ccgcccattg ccgcaggacc tcatcacggc
     1801 tcgcttgact taaatgagca acgtttggcc agcgcgataa aaacgcttca aaataaggtt
     1861 ttacggccgc gaccgtcgtc tgctgcagca taatttccga aagccagacc ttgtaagggt
     1921 ctgtgcattc gcctggcggc gcccgccacg gcaagtcccg gcgatggcag tcgtaccagg
     1981 cgagaagcgc attggctatg tcagcgcgtc ggtcgcggtg cgattggaag gccatagatg
     2041 cgcctcgttt tgaggctctc cgtcatgcag gcaaatgtca ggcaagacgg agggcgccga
     2101 agatgagcgc taagccaata cccgagggcc atgaagcggc aataccctat ttggccgtgc
     2161 gcaatgcgag cgacgcctta accttctaca aaaacgcatt tggcgcggtg gagacgcttt
     2221 gcatcgcgca tggcgccaaa attgggcatg ccgaactcct aattggcaag gccagggtga
     2281 tgctctgcga tgaatttcct gaccacgaag ttttgagtcc gcagacgatc ggcggcacgc
     2341 ctatcatgat ccatctttat gtcgatgatg tcgatgcatt cacgagccgg gcgatagacg
     2401 cgggccttaa ggtgctccgg ccggtcaagg atcagttcta cggggatcgc ggcggcaagt
     2461 tcgaggatcc ttttggccac cgttggtgga tcgccacaca caaggaggac ctcacgccgg
     2521 aggagctgaa aactcgcgcc gccgctctgt gttccaatgg cccagcctga acccctgtgg
     2581 gcgagagcag agtgagcact tgtctccaaa agtcttttcg tggagcatcg tcagaaatcc
     2641 ggtataagag gggatgcgaa accgtcccgg cgcaaaaatc ccgtggtccc ggccgctcgg
     2701 ggacctcgtg caatcggcaa tcgatccggt gttgacacgg agaggatttg gcctaacagc
     2761 gctcgttttg tattgggacg atattgttgg ggaaagactg gctgctgtgt ctcggccggt
     2821 tagggtgcaa tggccggcgc ggcaacgcga cccggcggtg ccggccgcgc tcgttgtgcg
     2881 tgtcgaaacg ggctgtgcgc tcgaattgca gcatctcgcc ccggtcattc ttgagcgtgt
     2941 gaatgctcat ttcggctggc ggtccatctc gcgtctcgtg ctgatgcagg gcccggttgc
     3001 cgcgccgccc gccgctcgtc gcgtggcgca gcccttgaat aaagccgcag aggccgcggc
     3061 ggcggagctt gttggcggag acctcgatca ttcgttgcgg ctggccttgg ttcggctcgg
     3121 tgcgcgcgtt ctcgccgggc cataattctg gttttgattg gtttcacctt ccctcgtcgc
     3181 tccaaaatac ccgccggagg tgagaggcag cgaacttgct atccatgcgc agcctcactc
     3241 atagcagctc atgccgaaaa gggttggccg tggtaatgga taggggagag ccgttgtaat
     3301 tgatacgggc gtaatgtacc gaatctcctg cggagggtat gctggtgagc aatagcccta
     3361 gtgttctgac tcattcattt gcgtcctgct ttggcgagaa tttttttggc tcggcggtgc
     3421 ttgacgagaa tggttttggc ggtggcggtc
//
LOCUS       DATMPU010000058         6021 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_2522537,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000058 DATMPU010000000
VERSION     DATMPU010000058.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 6021)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6021)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6021
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2522537"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..122
                     /locus_tag="VNY06_01325"
     CDS             <1..122
                     /locus_tag="VNY06_01325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012606126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HWX13514.1"
                     /translation="RIFKSHEDILDHCCFAWNKLIDMPWKIMSIGIRDWAYRS"
     gene            692..2188
                     /gene="guaB"
                     /locus_tag="VNY06_01330"
     CDS             692..2188
                     /gene="guaB"
                     /locus_tag="VNY06_01330"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014893003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HWX13515.1"
                     /translation="MALALITRPSPEEALTFDDVLLLPGHSQILPGAANLKTKLTATI
                     SLNIPIVSSAMDTVTEAKLAIALAQAGGIGVIHRNLDPVEQAEEVRKVKRYESGMVVN
                     PITVFPDETLADALALMRRHGISGIPVVERGPGGKPSRLCGILTNRDVRFADNPLQPV
                     SELMTKNLITVREGVSEQEARRLLHQHRLEKLIVVDDEFRCVGLVTVKDMEKATLHPA
                     ACKDAEGRLRVAAASTVGDQGYERALMLMDAGADCLVVDTAHGHSQAVLDQVKRLKRA
                     SNKAAVVAGNVATSDGAKALIDAGADALKVGIGPGSICTTRIVAGVGVPQLTAIMDCA
                     EVARDANIPVIGDGGIKYSGDLAKAIAAGAGCVMIGSLFAGTDESPGEVYLYQGRSFK
                     AYRGMGSIGAMALGSADRYFQQDINDQLKLVPEGVEGQVPYRGPISVILHQLAGGLRA
                     AMGYVGAADIEEFQARAQFIRISSASLRESHVHDVAITRESPNYPAGE"
     gene            2340..3632
                     /locus_tag="VNY06_01335"
     CDS             2340..3632
                     /locus_tag="VNY06_01335"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012590361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HWX13516.1"
                     /translation="MTPGARISAAIEVVEEIEARKRPAADVLKDWGHSHRFAGSKDRA
                     AIASLVYDGQRKRDSSAFIMGEETPRANVLGALQQMHNLSAGELTALCSGEAHAPPPL
                     SEEELERLCAGNLTEAPDFVAGDFPAWLRSSLDFVFGPALVAEMRALATRAPVDLRVN
                     TLKTSRDKVLNALKHLHAVPTPFSPSGLRLPVSPHGNGPKLSAEPAYIKGLVEIQDEA
                     SQLAAQLALAKPGEQVLDLCAGAGGKTLALAAMMQNKGQIHATDRDGARLMQGIARLK
                     RGGVRNVQLHTPSKDTNVLAGLEERCDLVFVDAPCTGTGTWRRNADAKWRIRPGALTV
                     RISEQSQALEEAARFVNPLGRILYVTCSVLREENEDRLAAFLATHSDYRTVPAPEMAK
                     MAGVPELSQFASRFGFGLRLTPLTSGTDGFFIAMLTRG"
     gene            3883..>6021
                     /locus_tag="VNY06_01340"
     CDS             3883..>6021
                     /locus_tag="VNY06_01340"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012590360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004474 - malate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006097 - glyoxylate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase G"
                     /protein_id="HWX13517.1"
                     /translation="MDYEQIGEIQVVKALRDFIGAEVLPGTDIGEAEFWSGLAGLISE
                     FAPRIRDQLRFRNELQDKIDEYHRATATMPFNPAGYAKFLREIGYIVPEPPNFTIRTE
                     NVLEIARVAGPQLVVPASNARYALNAANARWGSLYDALYGTDAIAETDGAERGKGYNK
                     VRGAKVIDWVRVLFDDLFPLARGSHKEVTAYRVSGGELIATLENGLETRLRHPGRFAG
                     YRKKSLDSFVVLLRHHGLHVEIEIDHNSLVGAESKAGVADVVFEAALSTIVDLEDSVA
                     AVDAQDKITVYRNWLGLMQGWLTATLEKNGLIFERKLAEDRTYIGRDGGSLTLHGRSL
                     LLVRNVGHHIDTDVVLDPAGNPIPETILDAVVTSLIAIHDLKRREGKRNSRHGSIYIV
                     KPKMHGPDEVALANDLFADVEDKLGLPRHTLKMGIMDEERRTSVNLKACIAAAADRVF
                     FINTGFLDRTGDEIHTSIEAGPMVRKNDMKNTDWIAAYENFNVDIGIACGLPGRAQIG
                     KGMWAAPDRMADMLAQKITHPLAGANTAWVPSPTAAVLHAVHYHRVDVASRWKVLARR
                     TPPLLTDLLAIPVCRANWAPDDVQAELDNNCQGILGYVVRWIDQGVGCSKVPDIHDIG
                     LMEDRATLRISSQHIANWLHQGVVTRELVMDTLRRMAQVVDRQNADDPLYQPMAPGFD
                     GHAFQAAVDLIFHGRAQPNGYTEWILHTYRR"
BASE COUNT         1278 a         1623 c         1824 g         1296 t
ORIGIN      
        1 accgcatctt caaatctcac gaagacattt tggaccactg ctgctttgcc tggaacaagc
       61 ttatcgacat gccttggaaa atcatgtcta tcggcatccg cgattgggct tatcggtcat
      121 aatcaatgag acttggtatt aatcctcagt tgggcgttgc ggtctgctgc ccagactgga
      181 gcggccgtgg gcgccgcaca agcttctgtg gagagtggct cgtgctggga ggctggtttg
      241 ggttgctaaa ggaacgtagg gccgatttct ttcaaaacat ctttgggatc tctgctacga
      301 agacgtttgc ccacgccatt tcctgggacg gaaagaacgg cggagttcct gaggaacaag
      361 atgctgtttc gtctccatcg ccgcggttcg gggttggtgc tgggaccatg ttttgtccgg
      421 gcgcggttga ggcatgaaac ggcgggattc gcctctcgcg ggcaaatgct cgtttatttt
      481 ttgcgaatca attttgtgcg cagaggctgc cttaggtcga attgggcgcg ttcagggcca
      541 gcagaggcgg cgaaagaagg cgagcgctct gggcatttgc tcaatggaaa gcaaggttcg
      601 cgcagctttg cgcctttgtc ccagcgtgtt atggagcccg cggttgattg acccgcgcat
      661 caagcccgcc gccgcggggc gcatcacgga gttggctttg gctctgatca cgcgaccctc
      721 gcccgaggaa gcgcttacat tcgatgacgt gctccttctt ccgggtcatt ctcaaatcct
      781 gccaggcgcg gcgaacctca agacaaagct caccgcgacg attagcctaa acattcctat
      841 tgtgtcttcg gccatggaca cggttacaga ggcgaagctc gcaattgcgc tcgcccaggc
      901 gggcgggatt ggcgtcattc accggaactt ggaccctgtc gagcaggccg aggaggtacg
      961 caaggtcaag cgttacgaaa gcggcatggt ggtcaatccg atcacggtct ttccggacga
     1021 aacccttgcc gacgcgctgg cgctgatgcg gcggcacggg atttccggca ttccggtagt
     1081 tgagcgcggg ccgggcggca aaccgtcaag actttgcggc atattaacca accgcgacgt
     1141 cagattcgca gacaacccgc tgcagccggt cagcgagttg atgaccaaga atctcataac
     1201 ggtccgcgaa ggtgtaagcg aacaagaagc gcgccggctg ttgcaccagc accggcttga
     1261 aaagcttatt gtcgttgacg acgagtttcg ctgcgttggc ctcgtaaccg tcaaggacat
     1321 ggaaaaggcg accttgcatc cggccgcctg caaagatgca gaggggaggc tgcgggttgc
     1381 tgctgcctca acggtcggcg accagggtta cgagcgcgcg ctcatgttga tggatgcggg
     1441 cgccgattgt ctcgtcgtcg atactgcgca tggccattcc caagctgttt tggatcaagt
     1501 caagaggctg aagcgcgcgt caaacaaggc tgcggttgtc gctggcaatg tggcgacatc
     1561 ggatggcgcg aaagccctga ttgacgcggg cgctgacgca ctcaaagttg gcatcgggcc
     1621 tggctcgatc tgcacaacac gtatcgtcgc aggagttggc gtgccgcagc tcaccgcaat
     1681 tatggattgt gccgaagtcg caagggatgc aaatattccg gtcatcggcg atggtggcat
     1741 caaatactca ggtgatctcg ccaaggcgat tgcggcaggc gccggttgtg tcatgatcgg
     1801 atcgcttttc gctggaaccg acgagagtcc aggagaagtc tatctctacc agggccgctc
     1861 gttcaaagcc tatcgaggca tgggctcgat cggcgcaatg gcgcttggat cggcagaccg
     1921 ctacttccag caggacatca acgatcagtt gaaactcgtg cccgaagggg tcgaggggca
     1981 ggttccctat cgcggtccga tatccgtaat tctgcatcaa ttggctggag gtctgcgcgc
     2041 cgcaatgggc tatgtcgggg ccgcggacat cgaggagttt caggcacgcg cgcagtttat
     2101 ccggatttcg tctgcgagct tgcgcgagag ccacgtccac gatgtggcca tcacccgcga
     2161 aagcccgaat tatccggcgg gggagtgaac ccgcatttga tcatattttt tttgagatga
     2221 cgcctggtaa tcaatcaagc ctttcttgtg ccagtatttg tggcgggcgg ttcatggcgc
     2281 ttcttgatgt ggctggtctt tgcaatatgc atcctttctg atttttttgc aggaacgcaa
     2341 tgacgcccgg cgcgcgcatt tccgcagcaa tcgaagttgt cgaagaaatc gaagcccgca
     2401 aaaggccagc tgcagatgtg ctgaaggatt ggggccattc gcaccgcttt gcgggatcaa
     2461 aggaccgtgc ggcgatagcc tcgcttgtct atgatggaca gcgcaagcgt gactcctctg
     2521 ctttcatcat gggcgaggaa acaccccgtg caaatgtgct cggtgctttg cagcagatgc
     2581 ataacttgag cgcaggtgag ctcaccgcgc tatgctcggg cgaagcacac gccccgcctc
     2641 cgctcagcga ggaagaattg gaacgcctgt gcgcgggcaa ccttacggaa gcaccggatt
     2701 ttgtcgccgg agattttccc gcatggctaa ggtcctcact ggattttgtg ttcggacctg
     2761 ccttggttgc ggagatgcgc gcgctggcga ctcgcgcgcc cgtcgatttg cgtgtgaaca
     2821 ccttgaagac cagccgagat aaggtgctca atgctctcaa gcatcttcac gctgttccaa
     2881 ccccattttc tccaagcggt ctgcgattgc cagtttcgcc gcatgggaat ggcccaaaac
     2941 tttctgccga gccggcctat atcaaaggcc tcgtcgagat tcaggatgaa gcctcgcagc
     3001 ttgcagcaca gctagctctg gcaaaaccgg gcgagcaggt gctcgatctt tgcgcgggtg
     3061 cgggtggcaa gacattggcg cttgcggcaa tgatgcagaa caaaggccag atacatgcga
     3121 ccgaccggga tggagcaagg ctcatgcaag gcatcgcgag acttaagcgc ggtggggtgc
     3181 gcaatgttca actccatacg ccgagtaaag acacgaacgt ccttgccggt ctcgaagaac
     3241 gttgcgatct tgtttttgtc gatgcgccct gtacgggaac ggggacgtgg cggcgcaacg
     3301 cggatgcgaa atggcgcatt aggccgggcg cgcttacagt ccggatctcg gagcaaagcc
     3361 aggcattgga ggaagccgct cggttcgtca accctctggg ccggattctc tatgtaacct
     3421 gttccgtcct gcgcgaggaa aatgaggata gacttgcagc gtttctcgcc acacattctg
     3481 attataggac tgttccggct ccggaaatgg ctaagatggc gggcgtgcca gaactcagcc
     3541 agtttgcctc tcgctttggt tttggcctac gtctgacgcc gctgacaagc ggaaccgatg
     3601 ggtttttcat cgccatgctc acacgtggct aaatcatgat tttgcagcat tcaggatagt
     3661 gcacgatttt tgcaataccg gtgacaagcg aaaatgcgag ggctgggtca tgacgaaagg
     3721 ccgcggttcg cgaaccatgg cactatgcca aactggaagt gcacggcatc ggcgcgtgcc
     3781 tccttggaat cggcgggcat cggggcgtgc aacaattgat caaagccaca tgaacagagt
     3841 agccgggtta agatgaactc atcgcagcaa gacatgaagc gcatggacta cgagcaaatc
     3901 ggtgagattc aagttgtgaa ggctttgcgc gactttatcg gcgctgaggt cctgcctgga
     3961 acggatattg gagaagccga gttttggagc gggctcgcag gtcttattag cgagtttgct
     4021 ccgcggattc gggaccaatt gcgattccgc aatgagctgc aggataagat agacgaatat
     4081 catcgcgcca ccgcaaccat gccgtttaat ccggcaggct acgcaaaatt tctgcgcgag
     4141 attggctata ttgtcccgga acctcccaac tttaccattc gcaccgagaa tgttttggaa
     4201 atcgcgaggg tcgcggggcc tcaactcgtg gttccagctt ccaatgcgcg ttatgccctc
     4261 aacgcggcaa acgcacgatg gggcagtctt tacgacgcac tctatggcac ggacgctatt
     4321 gccgagaccg acggcgcgga gcgcggaaag ggatacaaca aggtgcgtgg cgccaaggtc
     4381 atcgactggg tccgtgtgct ctttgacgac ttgtttcccc tcgcaagggg cagccacaaa
     4441 gaagtcaccg catacagagt ctcaggtggc gagctcatcg caactcttga aaatggactg
     4501 gaaacgcgac tgcgccatcc cgggcgtttc gccggatata ggaagaaatc gctcgactcc
     4561 tttgtcgtgc tgttgcgtca ccatggcctg catgttgaga tagagattga tcacaacagt
     4621 ttggttgggg ccgagagcaa agcgggtgtc gccgatgtcg tttttgaggc cgctctcagc
     4681 acgatcgtcg acctcgagga cagcgtggcc gcggtcgatg cgcaagataa gatcacagtc
     4741 taccgcaatt ggctggggct gatgcaggga tggttgacgg cgactctcga aaagaatggg
     4801 cttattttcg agcgcaagct agcggaggat cgcacctata tcggccggga tgggggctcc
     4861 ctgaccttgc atgggcgcag cctgttgctg gttcgcaacg tcggccatca tatagatacc
     4921 gatgtggttc tcgacccggc cgggaaccca attcccgaaa ccattctcga tgctgtggtt
     4981 acatcgctga ttgcgatcca cgatcttaag cgaagagaag ggaagcggaa cagccgccac
     5041 ggttcgattt atatcgtgaa gccaaaaatg catgggccgg acgaagtcgc gctcgccaat
     5101 gatcttttcg ctgatgtcga agataagcta gggcttcctc gccacacgct gaaaatgggc
     5161 atcatggacg aagagcggcg aacctccgtc aatctcaagg cctgcatcgc ggcggcagcc
     5221 gatcgcgtgt ttttcatcaa cactggcttt ctcgatcgca ccggtgacga aattcacact
     5281 tcgatcgaag ccgggccgat ggtccgcaag aacgacatga agaataccga ttggattgcc
     5341 gcctatgaaa atttcaatgt cgacattggc attgcttgcg ggctgccggg acgggcgcaa
     5401 atcggcaagg gcatgtgggc cgcgccggac cggatggcgg acatgcttgc gcagaagatc
     5461 acgcatccgc tggcgggagc gaatacggcc tgggttccat caccgaccgc cgcagtgtta
     5521 cacgcggtac attaccatcg agtcgatgtc gcgagccgct ggaaagtgct tgcccgccga
     5581 acccccccgc tcctgacgga tctccttgcc ataccggtct gccgggcgaa ctgggcgccg
     5641 gacgatgttc aagccgagct cgacaacaat tgccagggaa tcttgggcta tgtcgtgcgt
     5701 tggatcgatc agggcgtcgg ctgttcgaaa gtgccggata tccatgacat tggcctgatg
     5761 gaggatcgcg ccaccttacg gatttcaagc cagcacatcg ccaattggtt gcatcagggg
     5821 gtcgtgacgc gcgagctagt catggacaca ctccggcgaa tggcgcaagt cgtggaccgg
     5881 caaaatgcgg atgatccgct ctatcaaccg atggcgccgg gcttcgatgg ccatgctttt
     5941 caagccgcag tcgatctgat ctttcatggc cgcgcccagc caaatggcta cacggaatgg
     6001 atccttcata cttaccggcg c
//
LOCUS       DATMPU010000059        10377 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_1904124,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000059 DATMPU010000000
VERSION     DATMPU010000059.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 10377)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10377)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10377
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1904124"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..246)
                     /locus_tag="VNY06_01345"
     CDS             complement(<1..246)
                     /locus_tag="VNY06_01345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX13518.1"
                     /translation="MEELKVSKRLNPARAFVSRLIRKFEATRFAHSTDCEIWTLSPSD
                     AQFEISLREIGRLVPELNRTKRRMPSQRPNSLAVVTLI"
     gene            492..1637
                     /gene="rseP"
                     /locus_tag="VNY06_01350"
     CDS             492..1637
                     /gene="rseP"
                     /locus_tag="VNY06_01350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012592786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HWX13519.1"
                     /translation="MSMLTSFWSLAGYILPFLFVLSLVVFFHELGHFLIGRWCGVKVE
                     TFSLGFGPELFGFNDRYGTHWRVAALPLGGYVKFLGDANNTSMPDEAALEAMSPQERE
                     VSFSTQKVWKRAAIVAAGPIANFVLAIAIFGSIFYVHGRSILLPVVDTVAADSAAEAA
                     GFQPGDFIVSIDGAKIDSFEDMQRIVQGASDTQLLFVVDRAGKSVELVATPQRRDVAT
                     PFGNARVGVLGVQSKAKPENWHVQTYSFPESVRLAGSETWYVVARTGSYLKGLVSGRE
                     SADQLSGPIRIAEVSGEMAKIGLAALLNLAAILSISVGMLNLLPVPLLDGGHLVYYAV
                     EAMRGKAMNERAQQFGFKIGIMLVTSLMIFATYNDILRLTRQLMHWG"
     gene            1935..4457
                     /gene="bamA"
                     /locus_tag="VNY06_01355"
     CDS             1935..4457
                     /gene="bamA"
                     /locus_tag="VNY06_01355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003611197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HWX13520.1"
                     /translation="MAGAGFPMGYGRIFFGRITVAAAGLALLCALNLAAFAANVLVEG
                     NKRVDSETIAGYFTGSDQASINKGVKELYATGLFSSVKVGHAGGSVVITVVENNVINR
                     VVFEGNSKVKSETLTAEIQSKSRGPYSQAMVDADVERIKDVYRRSGRAAAAVTARTVE
                     LPNGRLDVVFTIDEGEKTGVKAINFVGNQAFSAGKLRDLMQTTEMNLFSFFKTSDIYD
                     PDRIASDLELIRRFYLKNGYADFRVVGSDAQFDAAQGGYIITITVEEGVQYTVGSANV
                     ESHLPSVDSASMQPFVRLSAGETYNGDLVQKTVDALTKELGKRGYPFTQVRPRGDRDP
                     ATRTVNIAFVLDDGPRVYIERIDVRGNTRTRDYVIRREFEIGEGDAYNKVLIDRAERR
                     LNNLGYFKKVRITNAPGSAPDRVIVLVEVEDQPTGSLSLSGGYSTTQGFIAEVSVSES
                     NFMGRGQFVRLALSEGQFSRGADFSFTEPYFLGERIAAGFDLYAKKTDSYQFSFYDNF
                     IVGGTVRFGFPITEELSFSPRYSIFRQDLSVPDNSSRPFNDCPSTGSPIFGITPGFTG
                     FPAVFNFNCLTNGEASLAIKEAQGPFLTSQFGYTIGYNSLDNIKSPTAGFFAELRQDI
                     AGAGGDARYVRTTGDLRYYHEIYDQIVGLIHLQGGDLQSFGGYQLRIVDNFNLGPNLV
                     RGFAPFGIGPRDVSFGLNPQGNPLGGTKYYGGSLEVQFPLYGMPRDLGLKGAFFFDAG
                     TLFGYTGRTVFPLSPLAALNVSPGTVCIPGFVPNNNVVLNFTQSNCISVGGDSSLIRT
                     SAGGSIIWASPLGPIRFDFAKAITKNQFDQTQFFRFTGGTTF"
     gene            4675..5556
                     /locus_tag="VNY06_01360"
     CDS             4675..5556
                     /locus_tag="VNY06_01360"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: protein motif:HMM:NF002060.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HWX13521.1"
                     /translation="MSELFFFPRAGETSLADIVSCTGAAVAEGADLSPLIRGVAPLDE
                     ARPGELTVLDSPEDAVLLEAGLATACFVTPSHAPFVPQTTLALVIDEPYLGFARAMSL
                     LFPQAIEAKSLFGTAGVNPGASIHPEARLEQDVIVDPGVVIGPRAEIGSNSIIGANSV
                     IGADVRIGRNCLISPQVTISHALIGDRVVLHPGVRIGQPGVPLAKVGIGLPSRNTARI
                     PAIGRVIIQDGVEIGANSTLDRGTLSDTVLGEETRVGSLVQIAENVTLGRFCTIAAQT
                     QIPAGTRRADFFSVPPE"
     gene            complement(5832..6530)
                     /locus_tag="VNY06_01365"
     CDS             complement(5832..6530)
                     /locus_tag="VNY06_01365"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003610952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HWX13522.1"
                     /translation="MSMIESVRKQLTPIHPEGYIFVVGFAIVTLVLGSFSGSLFWIGA
                     IATAWCAYFFRDPVRHTPLDVNLVIAPADGVVCSIGYFAPPPELGLSAEPIPRVSIFM
                     SAFDCHVNRAPVAGRVTKIAYRPGAFINADLDKASENNERNGLVIESAGSRFGVVQIA
                     GFIARRIVCFVRQGEVIGAGDRFGLIRFGSRVDVYLPAVARTLVTTGSRAIAGETVLA
                     ELRTTAPRRNFKSG"
     gene            complement(6695..8626)
                     /locus_tag="VNY06_01370"
     CDS             complement(6695..8626)
                     /locus_tag="VNY06_01370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018271078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HWX13523.1"
                     /translation="MGVAKSSARGTLRKLWPYIWPAGRPDLTARIFVTMALLLAGKVI
                     TIAVPFSIKWATDALSDAARAPLFSVIAGPLGFTLLYGLLRILMALLTQARDAIFADV
                     AMHAVRCLARDVFSHLHLLSLRFHLERKTGGLTRILERGRNSIETIVRTTVLTAVPTL
                     VEFFLILGIFLFQFDWRYAAVVTAMILAYMGFTTAATNWRIAIRRAMNESDTDANTKA
                     IDSLLNFETVKYFNAEEREEQRYDKSIARYERMSVKTYTSLAFLNAGQAVIFTLGLTA
                     AMTMTIHGIMSGANTVGDFVLINAMMIQLYQPLNFMGVVYREIRQSMVDIEMMFAILG
                     QRPEVEDRPGAKPLEVTDGRLSFEHVSFYYDARRSILEDVSFEVPAGKTVAIVGPSGA
                     GKSTLSRLIFRFYEPSAGRITIDGQDIAFATQTSLRAKIGVVPQDTVLFNDSIAYNIR
                     YGRFDATKAEVRDAARLARISHFIEQIPGGYEAQVGERGLKLSGGEKQRVAIARTILK
                     APPILVLDEATSALDTFTEREIQDALDIVAKGRTTLVIAHRLSTIVHADEIIVLDKGK
                     IVERGAHGALLARNGLYAAMWKRQYEVRQAEETLRRAAIEEGARLSIKIESEELAEAD
                     SEREEGNPPEKDEEISPDL"
     gene            8872..9507
                     /locus_tag="VNY06_01375"
     CDS             8872..9507
                     /locus_tag="VNY06_01375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020176737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HWX13524.1"
                     /translation="MALNISIGEADSRLVRNICVYCGSAKGADPIYEAAARRLGETMA
                     KTGVGLVFGGGAVGLMGTIAGSVLANGGRVTGIIPNFLVGREGLLQDLEDYLIVDGMH
                     ERKRLMFERADAFVALPGGIGTLEELTEQLSWVQLERHTKPVVIADIGGFWKPLLDLF
                     AHMRERRFIQSPFEVRYLVAEKIEDILPMVETAAGKAAVRGAAKRTVDPRL"
     gene            9601..>10377
                     /locus_tag="VNY06_01380"
     CDS             9601..>10377
                     /locus_tag="VNY06_01380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX13525.1"
                     /translation="MTLEEEIPDADLKTDLSERLIAPAEHRARAPFLLLLGACFLAHA
                     LILGFLLFEDFFLAPGPTVAEEIPVEIVAELPPEEKTEPIVKPLPPPPPDLVTPPVVK
                     AAPPPEKVQLEDVKVAYDAPASGNASQVQKAEVSKETQAPRQAPPPKLVVPQPPQDKL
                     EQENAATPANEKPAEISLPSEPQKLFDDRPDAEALDRAQPEPPAPKPKEKTAQKPSKD
                     TAAQGTKATVAQQLAALAPAPEFSFEAKAKAAQIGGGTEKA"
BASE COUNT         2251 a         2948 c         2900 g         2278 t
ORIGIN      
        1 gattaatgtc accacagcaa gggagtttgg gcgctgcgac ggcattcggc gcttggtcct
       61 attaagctct ggtaccagcc ttccaatttc gcggagggat atttcgaatt gagcatctga
      121 tggggaaagc gtccagattt cgcagtcggt tgaatgtgcg aaccgagtag cctcaaactt
      181 tctgatcaga cggctgacaa aggcgcgcgc cggatttagt ctttttgaaa ctttcagttc
      241 ttccattatc tcaaagagtt ctgaaacgcc ggaccctccg ctaaaattcg ctcgctcatt
      301 ataaggggca acattggcga ggaactcgct tctcgataga accgcaaaga aaattacggc
      361 ccgtcacggc gacaacgctt agcgccgcat tcggccaagc cccaagaaac ttgagcatgg
      421 cacgagcgtt ggcggggccg gaattatcgt cgagccgcgc tgacaaattt atactgcgag
      481 ccaggagtca aatgtccatg ctcacatctt tttggtcgct cgccgggtat attttgcctt
      541 ttctttttgt cttgagcttg gtggttttct ttcatgagct tggccatttt ctgatcggcc
      601 ggtggtgcgg ggttaaggtc gaaacctttt cgctcggctt cggccccgag ctctttggct
      661 tcaatgaccg ctacggcacc cattggcggg tcgcggctct gccgcttgga ggctatgtca
      721 agttcctcgg agacgccaat aacacctcaa tgccggatga agccgccctg gaggcgatgt
      781 ctccgcaaga acgagaggtg agtttttcca cccagaaagt ctggaagagg gcagcgattg
      841 ttgccgcggg gccgattgcg aatttcgttc tcgcgatcgc aattttcggt agcatatttt
      901 acgtgcatgg ccggtcgatc ctgctccctg ttgtcgacac tgtcgccgca gacagcgccg
      961 cggaagcggc tgggtttcaa ccgggggatt tcatcgtttc cattgatggc gccaaaattg
     1021 atagtttcga ggatatgcag cggatcgtac agggggcaag cgacacgcag ctgctctttg
     1081 tcgtcgaccg ggcggggaaa tctgtcgagc ttgtcgcgac gccacagcgg cgcgatgttg
     1141 caacgccttt cggcaacgcg agggttggtg tgctaggcgt ccaatccaag gccaagccgg
     1201 aaaattggca cgtccagacc tatagtttcc cggaatcggt aaggcttgcc ggatcggaga
     1261 cctggtatgt cgttgcgcgt accggcagct atctcaaagg cttggtttcc gggcgggaat
     1321 ccgccgacca gctttcgggc cccatccgga tcgcggaggt ttcgggagag atggccaaga
     1381 ttggccttgc ggccctgctc aaccttgccg caattctgtc gatttcggtt ggaatgctca
     1441 atcttctgcc ggttccgctc ctcgatggcg ggcatcttgt ctactatgcc gtggaagcca
     1501 tgcgcggcaa ggccatgaat gaaagggcgc aacagttcgg gttcaagatc ggcatcatgc
     1561 tggtcacctc gctgatgata tttgccacct ataacgacat ccttcgcctg acgcgccaat
     1621 tgatgcattg gggatgaccg agagggccaa gaggcccggc gcagtgcccg cacggcttca
     1681 gaaatagttg aattaacgcg gtcctcagct caataacagc ggaccgttaa ccctgttcct
     1741 taagcaattg ctcacaggat gcgtgtcttg tctgccacaa tctcgcaaaa accttaaggt
     1801 attgcgaaac gtgaattgtg cgctgaggca aagctggtca aaacaacatt cgggaggtgg
     1861 caagggaatc cttcacctct tcgttacgac gagttgttta tcagcccgcg tcaggtttgg
     1921 agtgagcgtt cgtgatggcg ggggccggtt ttccaatggg atatgggcgt attttttttg
     1981 gccgcatcac cgttgcggct gccgggctgg cgttgctgtg cgcattgaac ctcgcagcct
     2041 tcgccgcgaa cgtattggtg gaaggcaaca agcgcgtcga tagcgaaacg attgccggtt
     2101 acttcacagg ctcggatcag gcctcaatca acaagggcgt caaggaactc tacgccaccg
     2161 gtttgttttc cagcgtcaag gtcgggcacg cgggcggcag cgtcgtgatc accgtcgttg
     2221 aaaacaacgt gatcaatcgt gttgtcttcg agggcaatag caaggtcaag tcagagacac
     2281 tcacagccga gatccagtca aaatcgcggg gtccctatag tcaggccatg gtcgacgcgg
     2341 atgtcgagcg catcaaggat gtctatcgcc gctcaggccg cgccgcggca gcggtgacag
     2401 cccggactgt cgaactgcca aacggtaggc tcgacgtcgt tttcaccatt gatgaaggcg
     2461 aaaagacggg tgtgaaagca atcaatttcg tcggcaatca agcattttcc gctggcaagc
     2521 tgcgggatct gatgcagaca acggaaatga atctgttctc gttctttaag acttccgaca
     2581 tttacgatcc cgatagaatt gcctcggatt tggagctcat tcgccgcttt tatctcaaaa
     2641 atgggtatgc cgattttcgc gttgtgggtt ctgatgccca gttcgacgcc gcgcaaggtg
     2701 gctatatcat caccatcacc gtcgaggagg gcgtgcagta cacggttggc tcagccaatg
     2761 ttgaatccca cttgccaagt gtcgatagtg cgtcgatgca gccgtttgtg cgcctttcgg
     2821 ccggcgaaac ctacaatggc gatttggtcc aaaaaaccgt cgacgccttg accaaggagc
     2881 taggcaaaag gggctacccg ttcacccagg tgcgcccgcg cggcgatcgc gacccggcaa
     2941 cccgcaccgt gaacatcgcc ttcgttttgg atgatgggcc tcgcgtctat attgagcgca
     3001 ttgacgtgcg cggcaatacg agaacgcgcg actatgtaat ccgccgcgag ttcgaaattg
     3061 gcgaaggtga tgcctataat aaggttttga tcgaccgagc agagcggaga cttaataatc
     3121 ttggatattt caagaaggtg cggatcacca atgcgccggg ttcggcgccc gaccgggtaa
     3181 tcgtgctcgt cgaggtcgaa gatcaaccga ccggctcttt gtccctgtcc ggaggctatt
     3241 ccaccacgca aggctttatc gccgaggtct cggtatccga atccaatttt atgggccgcg
     3301 gtcagttcgt gcgcctcgcg ctatcagaag ggcaattcag ccgcggcgcc gacttcagtt
     3361 tcactgagcc ctactttctc ggcgaaagga tcgcggcagg cttcgatctc tatgccaaga
     3421 aaaccgattc ctaccaattt tcattttacg acaacttcat agtcggcggg acagtgcgct
     3481 ttggttttcc gattactgaa gagctgtcgt tctcgccgcg ctattcgatt ttccgccagg
     3541 acctctctgt gcccgataat tcttcgcgcc cgttcaacga ttgtccgagc acgggcagcc
     3601 ccattttcgg gataacgccc ggatttaccg ggttcccggc ggtgtttaat tttaactgcc
     3661 tgacgaacgg cgaggcatcg ctcgcgatca aggaggcgca aggtccgttt ctgacttcgc
     3721 agttcggata tacgattggc tacaattcgc tcgataatat caagagcccg acggcaggct
     3781 tctttgccga gctccgccag gacatcgccg gcgcgggcgg cgatgcacgc tatgtgcgca
     3841 cgaccgggga tcttcgctat tatcacgaga tatacgatca aattgtcggg cttattcacc
     3901 tccagggcgg cgacctgcag tcttttggcg gataccaatt gcgcattgtc gacaatttta
     3961 acctcggtcc aaaccttgtg cgcggtttcg ctccattcgg tattggcccg cgcgacgtct
     4021 cgttcggcct caacccgcaa ggcaatccgc tcggggggac caaatattat ggtggcagtc
     4081 tcgaagtgca gtttcccctc tacggtatgc caagagatct cggtctcaag ggagcattct
     4141 tctttgatgc gggcacgcta ttcggttaca ccggtcgaac cgtatttcct ctttcgcccc
     4201 tggcggcgct caacgtttcg ccgggaactg tttgcattcc aggatttgtg cccaacaata
     4261 atgtggtgct aaactttacg caaagcaact gtatcagtgt tggaggcgat tcttcgctga
     4321 tccgcacctc cgccggaggc tcgatcattt gggcctcccc gctcgggcca ataaggtttg
     4381 acttcgccaa ggccatcacc aaaaaccaat tcgatcaaac tcagttcttc cgttttaccg
     4441 gcggcaccac tttctgactt cctggagcaa gacggcattt cttcaaatct cttatcgcgg
     4501 gccgggacga aatttcggtt cccttttgct cgacacgctt tagcgtctca tcgctccgtg
     4561 cgacgttcgc cacgagacgc gttcgcgtcg agtgctattc cgcgccgcta ccaccttttt
     4621 cctattgtag ctcttctcct tcaggaacta ggaaataagg cgtggagagg tttcatgagc
     4681 gaactattct ttttcccccg tgccggcgaa acatcactcg ctgacatcgt ttcttgcacc
     4741 ggcgcggcgg tcgctgaggg tgcggatcta tcgccgctca taaggggcgt ggcgccgctc
     4801 gacgaagcgc gaccgggaga actcaccgtc ctcgattcgc cggaagacgc agtgcttttg
     4861 gaggcaggcc tcgcaacggc atgttttgtt acgccgagtc atgcgccatt cgtgccgcaa
     4921 acgaccttgg cactcgtcat agatgagcct tatcttggct tcgcgcgtgc catgagcctc
     4981 ttgttcccgc aggcgatcga ggcgaaatcc ttgttcggta cggcgggagt aaatccggga
     5041 gcttcaatcc atcccgaagc gcgcctcgaa caagatgtga ttgtcgaccc cggcgtcgtc
     5101 atcgggccgc gcgcggaaat aggctccaat tcgatcattg gcgcgaacag cgtcattgga
     5161 gcggatgtcc gaataggtcg gaattgcctc atatcaccgc aggtgaccat tagccatgcg
     5221 ctcatcggcg acagggtcgt attgcatcct ggcgtgcgca ttggacagcc aggagtccca
     5281 ttggccaaag tcggaatcgg cctgccctcg cgcaataccg ccagaatacc agcgattggc
     5341 cgggtcatta ttcaagacgg ggtcgagatc ggcgccaatt ccaccctaga tcgcggcacc
     5401 ttaagcgata cggttctcgg cgaggaaacc agggttggca gtcttgtcca gatcgcggag
     5461 aatgtcacgc tcggacgctt ttgcaccatc gcggcgcaaa cgcagattcc agccggcacg
     5521 cggcgcgcgg atttcttttc tgtcccgccc gaatgatcga atagtgaagc atcgagggcc
     5581 ccggtcccct cgtttagagc gagatgactt tgcttcgaaa cgttatcttg cgccaaatcc
     5641 tttatttgcc gcgcgtccga gcgcaatacc gggttccgct tttagctcgg gcacgccggg
     5701 cttgggccgc tctgcaagcg ccaaagatgg agcctcgata ctcaggcaaa acgccggatt
     5761 aagttccaag ccgggcaggc gaaaaacggg gcaaatgagg cgaatggtgg cagagccttt
     5821 ttgacaagcc ctcagccgga cttgaaattg cgccgcggcg cggtggtccg aagctcggcg
     5881 agcaccgtct cccctgcgat cgccctcgag cccgtcgtca ccaaggtacg cgccacggct
     5941 ggcagataga catcgacacg cgaaccaaag cggatcagtc caaacctgtc gccagcgccg
     6001 ataacctcgc cctggcggac gaagcagaca atgcgacgcg caataaatcc cgcgatctgc
     6061 acgacgccaa agcggctacc ggcactttcg atgacaaggc cgttgcgttc gttgttttcg
     6121 ctcgccttgt cgagatcggc attgatgaag gcgcctggcc ggtaggcaat tttcgttaca
     6181 cgcccggcaa caggagcccg gttcacatgg cagtcgaaag ccgacatgaa gattgatacc
     6241 cgcgggattg gctccgcgct caagccaagc tccggcggcg gcgcgaaata gccaatggag
     6301 cagacaacgc catctgcggg cgcgattaca agattcacgt caaggggggt atggcggacc
     6361 ggatcgcgaa agaaataggc gcaccaagcg gtcgcgatcg ccccaatcca gaaaagagag
     6421 ccagagaaag agccgaggac aagcgtgacg atcgcgaagc ccacgacgaa aatataaccc
     6481 tcgggatgga tgggcgtgag ttgctttcgg accgattcga tcatcgacat gggcaatcct
     6541 atctcactta aatattagcc aagggctccg ccaatagaaa gtcctgcccg cgcggttcta
     6601 ttcagtcgcc tcagctaagc taaacgcaac agccgctggg gcacggtaaa atcactcaaa
     6661 aatgaaatca ctttccggtt gtattctccc tcgatcaaag atcgggcgaa atttcctcat
     6721 ccttctccgg cggatttccc tcctcccgct cactgtcagc ctccgccaat tcctctgatt
     6781 ctatcttgat gctgagccta gcaccctctt cgatcgcggc gcggcgcaag gtttcctcgg
     6841 cttgcctcac ctcgtactgg cgcttccaca tcgccgcata gaggccgttt cgtgcaagaa
     6901 gtgctccatg tgcgccgcgc tccacgattt tgcccttgtc gagcacaatt atctcgtcgg
     6961 catggacaat cgtcgaaagc cggtgcgcaa tcacaagggt cgtccttcct ttggcgacaa
     7021 tatcgagcgc atcttgaatt tcgcgttcgg taaaagtgtc gagcgccgat gtcgcctcat
     7081 cgagaacaag aatgggcggt gcctttaaga tggttcgcgc aatcgcgacg cgctgttttt
     7141 ctccgccgga aagttttaat cctcgctctc caacttgcgc ctcatagccg cccggaattt
     7201 gttcaatgaa atggctgatc cgcgccaaac gcgcggcgtc gcgcacttcg gctttggttg
     7261 catcaaagcg gccatagcga atgttataag cgatcgagtc gttaaaaaga accgtgtctt
     7321 gcggaaccac accgatcttt gcgcgcaatg atgtctgcgt cgcaaacgca atatcctgcc
     7381 catctattgt aattcgcccg gcagaaggct catagaagcg gaatatcagc cgcgaaagcg
     7441 tggatttgcc agcacccgac gggccgacga tcgccacggt cttgccggcc ggcacttcga
     7501 agctcacatc ctccagaatc gaacggcgcg cgtcgtaata aaaagacaca tgctcgaaag
     7561 aaagcctgcc gtccgtcact tcgagcggct ttgcgccggg gcggtcctcg acctcgggac
     7621 gctggccgag aatggcaaac atcatttcga tatcgaccat cgactgcctg atctcgcgat
     7681 agacgacgcc catgaaattg agaggctggt agagctgaat catcatcgcg tttatgagaa
     7741 cgaaatcgcc gaccgtattg gcgccgctca ttatgccatg gatggtcatc gtcatcgcgg
     7801 ccgtcagccc aagcgtgaaa atgacggctt ggcccgcgtt gagaaaggca agagaagtgt
     7861 atgtcttgac gctcatgcgc tcatagcggg cgatggattt gtcgtagcgc tgctcctcgc
     7921 gctcttccgc attaaaatat ttcacggtct cgaaattaag caagctgtcg attgcctttg
     7981 tgtttgcatc agtgtcgctt tcgttcatcg cacgccggat tgcgatccgc caattggtgg
     8041 cggccgtcgt gaatcccata taggccagga tcatcgcagt cacgaccgcg gcatagcgcc
     8101 aatcgaattg aaaaagaaaa atccccaaaa tcaggaaaaa ttcgacaagc gtcggaaccg
     8161 cggtcagcac ggtcgtgcgc acgatcgtct cgatcgaatt gcggccgcgt tcgagaatcc
     8221 ttgttaggcc gcctgtcttg cgttcgagat gaaagcgcag cgacagcaag tgcaaatgcg
     8281 aaaatacgtc gcgcgcgaga cagcgcaccg catgcatcgc aacatcggca aagatcgcat
     8341 cgcgcgcctg ggtaaggagt gccattaaga tgcgcagtaa gccgtaaaga agggtaaagc
     8401 caagcggacc cgcgataacg ctaaaaagcg gagcccgcgc ggcatcgctc aaggcatccg
     8461 ttgcccattt gatggaaaag ggaacggcga tcgtaatcac cttcccggcc agcagcagag
     8521 ccatggtgac aaaaatccgc gccgtcaaat ccggacgccc ggcgggccag atatagggcc
     8581 aaagcttgcg aagcgttccg cgcgcagacg atttggcgac tcccatcgcc tgacgcgctt
     8641 gggctttgaa aatgaaaagt gaggacatcg gactcgatta ctggcggagg ctcattctgg
     8701 acccagcaca tccgaatgct atagataatt catcggcgag gtgcgtcatc taagtttttt
     8761 gattaggcca taacacgctc tagagtaatg gttgcatccg ctgcggccca ccgtagcgcc
     8821 tccggttatt acgagccgga ccaatttcac gcggcacaca acaacaggaa catggcatta
     8881 aatatcagca tcggagaggc tgactcccgc ctcgtccgca acatttgcgt ctattgcggt
     8941 tctgccaagg gtgcggaccc aatttacgaa gcagccgcgc gaaggcttgg cgagacaatg
     9001 gcaaagaccg gggtcggtct cgtttttggc ggcggcgcgg tcggactcat ggggacaatt
     9061 gccggatcgg ttctcgccaa cggcggcagg gttactggca ttattcctaa cttcttggtt
     9121 ggccgggagg gactccttca agaccttgaa gactatctca ttgtcgatgg catgcatgag
     9181 cgcaagcggc tgatgtttga gcgtgccgac gcctttgttg cacttcccgg cggcatcgga
     9241 acattagaag agctcaccga gcaattaagt tgggtgcagc ttgagcgtca caccaagccc
     9301 gtagtgattg ccgacattgg ggggttctgg aaacccctgc tcgatctctt cgcccatatg
     9361 cgcgaacggc gttttatcca atcacctttc gaggttcgct atcttgttgc cgaaaagatc
     9421 gaggatatct taccaatggt tgaaaccgcc gcgggcaagg ccgcggtccg gggggcagcc
     9481 aaacggaccg tcgatccgag gttgtgatcg cttgattgag acgaaagcta tagcgtagcc
     9541 ttaattcagc caagccacga tcttgttcgc gacgacagca ggaagcgatt gggggagcgc
     9601 gtgacgctcg aagaagagat ccccgatgcg gatttgaaga ccgatctgtc ggaacgcctc
     9661 attgcgccgg cggaacaccg cgcacgtgcg ccattcttgc ttctccttgg agcgtgcttt
     9721 cttgcccatg ccttgatctt aggtttcctt ctatttgagg atttcttcct cgctcccggt
     9781 cccactgtcg ctgaagagat accggtggaa atcgtcgccg aacttccgcc ggaggaaaag
     9841 accgaaccca ttgtcaagcc tctccccccg ccgccgcctg atcttgtgac tcccccggtg
     9901 gtcaaggcgg cgccgccgcc cgaaaaggtg cagctcgagg atgtaaaagt cgcttatgat
     9961 gcccccgcgt cgggcaatgc cagccaagtt caaaaggcgg aagtatccaa ggaaacacaa
    10021 gctccgcgcc aagctccgcc gcccaaactc gtggtgccgc agccgcccca agacaagctc
    10081 gagcaggaaa atgccgcgac tccggcaaat gagaagcccg ccgagatctc tttgccttcc
    10141 gagccgcaga aacttttcga cgaccggcca gacgccgagg ctctcgacag agcgcagccg
    10201 gagcctccgg cccctaaacc caaagaaaag acagctcaga aaccgagtaa ggataccgct
    10261 gcccagggca ctaaggctac ggttgcgcag cagctcgcag ctttggctcc tgcccccgaa
    10321 ttttccttcg aggccaaagc caaggcagcg cagattggcg gcgggacgga aaaagca
//
LOCUS       DATMPU010000060         3066 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_1595427,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000060 DATMPU010000000
VERSION     DATMPU010000060.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 3066)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3066)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3066
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1595427"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..575
                     /locus_tag="VNY06_01385"
     CDS             <1..575
                     /locus_tag="VNY06_01385"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HWX13526.1"
                     /translation="DFTKAIGLDPKDAVAYRLRAAAYSKKGDRDRAIKDYHKAIDLYG
                     QAIQRDAKTAALWNSRCWTRAIVGELNEAQSDCSESLDLEPNNANAHDSRAFVYLKMG
                     KAEDAVAEYNIAVGLDPSLAGSLYGRGVAKLKKGDKDGGSSDIESAKRIQPDIADEFQ
                     RYGIQLSQLSQSSPHSQTSRARRSRPQADR"
     gene            complement(499..1197)
                     /gene="purQ"
                     /locus_tag="VNY06_01390"
     CDS             complement(499..1197)
                     /gene="purQ"
                     /locus_tag="VNY06_01390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020173878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HWX13527.1"
                     /translation="MKAAVILFPGSNREQDALCALDKVANARPSIVWHGAHSLPHGTD
                     LVVLPGGFSYGDYLRCGAIAARAPIIDAVRAHAERGGLVLGICNGFQILVETGLLPGV
                     LMRNANLHFICRMQHLTVERNDTPFTSRYAKGQIIKVAVAHGEGNFTADAETIARLEA
                     EGRVAFRYCDAQGGRNADANPNGSVNDIAGIYSEGFNVLGLMPHPENLIDPLVGGTDG
                     RGLFESVATIARVD"
     gene            complement(1260..1499)
                     /gene="purS"
                     /locus_tag="VNY06_01395"
     CDS             complement(1260..1499)
                     /gene="purS"
                     /locus_tag="VNY06_01395"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007565237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HWX13528.1"
                     /translation="MKARVVVTLKNCILDPQGKAIEGALKSLGINGVDSVRQGKIFDI
                     ELAISDRNAAEDLLRGACEKLLANQIVENFRVELV"
     gene            complement(1568..2362)
                     /gene="purC"
                     /locus_tag="VNY06_01400"
     CDS             complement(1568..2362)
                     /gene="purC"
                     /locus_tag="VNY06_01400"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010684691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HWX13529.1"
                     /translation="MDHLKPRLIPMNRRRRIYEGKAKVLYEGPEPGTLIQHFKDDATA
                     FNAKKHEVIDGKGVLNNRISEFVFQNLNDIGVPTHFIRRLNMREQLIREVEIVPLEVV
                     VRNVAAGSLSTRLGIEEGTQLPRSIIEFYYKNDQLNDPLVSEEHITAFGWATPPEIDD
                     IMALAIRVNDFLSGLFLGVGIRLVDFKMECGRLWEGETMRIVVADEISPDSCRLWDIK
                     SNDKLDKDRFRRDLGGLVEAYTEVARRLGILQENEQPRAHGPKLVQ"
     gene            2737..>3066
                     /locus_tag="VNY06_01405"
     CDS             2737..>3066
                     /locus_tag="VNY06_01405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012045345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1476 domain-containing protein"
                     /protein_id="HWX13530.1"
                     /translation="MPVRNGFLGTNMTTFDERENAFEEKYAHDEELKFKAKARRDRLL
                     GLWAAEKLGKAGAAAEQYAARLVAAEVEKDSLGQILTTIRTDFDGAGIVIPDREIYKK
                     MDELLALA"
BASE COUNT          722 a          838 c          855 g          651 t
ORIGIN      
        1 acgatttcac caaagccatc ggcctcgatc cgaaagacgc cgtcgcctat cgtttgagag
       61 ctgctgctta ttcgaaaaag ggcgaccgcg atcgcgcgat caaagattac cacaaagcta
      121 ttgacctcta tggtcaggcg atccagcgtg atgccaaaac cgctgcctta tggaatagtc
      181 gctgttggac gcgcgcaatt gtcggcgaat tgaatgaggc acaatcggac tgcagcgagt
      241 cactggatct ggaacccaac aatgctaatg cccacgatag tcgcgctttt gtctacctaa
      301 aaatgggcaa agccgaagat gccgtagctg agtacaacat agcggtgggg ctagacccga
      361 gccttgcagg ctcactctat ggccgcggtg tcgcgaagct gaagaaggga gataaggacg
      421 gtgggagttc cgatattgaa tcggcgaaaa gaatacaacc agatattgca gacgagttcc
      481 agcgctacgg aatacaactc agtcaactct cgcaatcgtc gccacactct caaacaagcc
      541 gcgcccgtcg gtcccgccca caagcggatc gatgagattt tcgggatgcg gcatcaggcc
      601 cagcacattg aacccttccg aataaatccc ggcgatatca ttgaccgagc cgtttggatt
      661 ggcatccgca ttcctgccgc cttgcgcgtc gcaatagcgg aaggcaactc ggccctcggc
      721 ctcgagccgc gcgatggtct ctgcgtcggc cgtaaaattg ccttcgccat gcgcgactgc
      781 cactttgata atctggccct tggcataacg agaggtgaag ggcgtgtcat tacgctcgac
      841 cgtcagatgc tgcatgcggc agataaaatg cagattggcg ttgcgcatca atacgccggg
      901 taacaggccg gtctcaacca gaatctgaaa gccattgcag atgccgagaa cgagaccgcc
      961 ccgttcggca tgagcgcgta cggcgtctat gatcggcgcc cgcgcggcga tggctccgca
     1021 gcgcagataa tcgccatagg aaaaaccgcc gggaagcacg acaagatcgg tgccgtgggg
     1081 gagagaatgc gcgccatgcc agacgatgga aggtctagcg ttagcaactt tatccagggc
     1141 gcacagcgca tcctgctcgc ggttcgagcc cggaaagaga atgacggcgg ccttcatgcc
     1201 tcgacgatat attccgtgct caagatcgct cgcctagaca cgcaggtggt tgcaacagct
     1261 tatacaagct ccactcgaaa attttcaacg atctggttgg caagcagttt ttcgcacgct
     1321 ccgcgcagca ggtcttccgc tgcgttgcga tcgcttatgg cgagttcgat atcaaaaatc
     1381 tttccctgcc gcacgctgtc gacaccatta atgccgagcg atttcagcgc gccctcgatg
     1441 gccttgccct ggggatcgag aatacagttc ttgagcgtga caacaacacg cgctttcaca
     1501 ggtttgatcc cggttcattg gtcaagataa ggaatcgtcc gccggcggcg ctgacgaggc
     1561 gagacaatca ctggacgagc tttggcccat gggcgcgcgg ctgctcgttt tcctgcaaga
     1621 tgccgagccg tctggcgact tccgtatagg cctcgaccag cccgccgagg tcgcggcgaa
     1681 aacgatcctt gtcgagcttg tcgttcgact tgatgtccca cagccggcaa gaatctgggg
     1741 aaatctcatc ggcgacgaca atccgcatcg tctcgccttc ccaaagcctg ccgcattcca
     1801 tcttgaaatc gaccagccgt atgccgacac cgaggaaaag gccggagaga aaatcattga
     1861 ccctgatcgc gagcgccatg atgtcgtcga tttccggcgg cgtggcccag ccaaaggccg
     1921 tgatatgctc ttccgagacc aaaggatcgt taagctgatc gtttttgtaa tagaactcga
     1981 tgatcgagcg tggcagttgc gtgccttcct cgatcccgag acgggttgag agggatccgg
     2041 ccgcgacatt gcggacaacg acctcaagcg gcacgatttc gacttcccga atcaattgct
     2101 cgcgcatgtt gaggcggcgg atgaaatggg tcggcacgcc gatgtcattg agattttgaa
     2161 aaacaaactc ggaaatccga ttgttgagca ccccttttcc gtctatgact tcatgtttct
     2221 tggcattaaa cgcggttgcg tcgtccttaa aatgctggat gagcgttcct ggttcgggac
     2281 cctcgtagag gacctttgcc ttaccttcgt agatgcgccg gcggcgattc attgggatta
     2341 accgtggctt gagatgatcc atgggtggcc gtggctcctt tgaacgtcct aaatcccgca
     2401 tccagatgcg ctggcgcgca agagggggac ggggaggtag gctctgcgct gttttgccgc
     2461 cacgacatca agggaaaccc tgcttgttcc cattctgcaa caagccggac gaaccaaact
     2521 atccgaaagc agaaattctc acaatgccgt gctaactttc acactaggcg ggcaaatcga
     2581 gatatttata tatgattgtt cccctcctta ttagaaacgg acgattatct ttagcagaga
     2641 ttcgtaacgg gacataggaa gaattcgagc tttaacgtgg ctttgctcat tatagtgccg
     2701 ccgcggattt ttgctattat cgcgcacata gcttagatgc cggttcgcaa cggtttcctg
     2761 gggacgaaca tgacgacttt cgacgagcgc gagaatgcct tcgaagagaa atacgcccat
     2821 gatgaagaac tgaaattcaa ggcaaaagcg cggcgcgaca ggttgctcgg tctttgggca
     2881 gcagagaaac tcggcaaggc aggcgcggca gcggagcaat atgctgcccg cctcgttgcc
     2941 gcggaagttg agaaagacag cctgggccag atccttacga ccatacgtac cgatttcgat
     3001 ggcgccggga tcgtcatacc ggaccgcgag atatacaaga aaatggatga gttgcttgcg
     3061 ttggca
//
LOCUS       DATMPU010000061         4001 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_3144448,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000061 DATMPU010000000
VERSION     DATMPU010000061.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 4001)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4001)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4001
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3144448"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..303
                     /locus_tag="VNY06_01410"
     CDS             <1..303
                     /locus_tag="VNY06_01410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015596458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HWX13531.1"
                     /translation="NLSSGTLRPRPSRSSRKGKPSQMFGEVWQWTQSAYLPYPGYRAP
                     QGALGEYNGKFMVGQQVLRGASCVTPAGHTRASYRNFFYPHQRWQFAGLRLASEFL"
     gene            303..1340
                     /gene="egtD"
                     /locus_tag="VNY06_01415"
     CDS             303..1340
                     /gene="egtD"
                     /locus_tag="VNY06_01415"
                     /EC_number="2.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020174854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030745 - dimethylhistidine
                     N-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0052699 - ergothioneine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-histidine N(alpha)-methyltransferase"
                     /protein_id="HWX13532.1"
                     /translation="MPKSNFALRKTAPWPNSEFATSVMNGLSKRCKSLPCKYFYDARG
                     SELFEKITNLPEYYPTRTEIAILEVHAAEIANGVPDRAILVEFGSGSSRKTEILLAAL
                     PGLCAYVPIDVSESALHGAKQRLQKRFPTLEVHPIVADFSRSATLPSNLASLPKLGFF
                     PGSTIGNFSPPSAVRLLRAMRDMLSPEGHLIIGVDLKKEARKLVCAYDDSEGTTAAFN
                     LNLLARINRELEGTFDLEGFGHEAIYNPREGRIEMRLVAKKAQAASILGRRFQFFDGE
                     IIHTENSYKYCTGQFQELARAAGWHPNRLWSDEQNLFSVHELISKTSFGTAIRCALFN
                     WLPGERGDSAA"
     gene            1486..2211
                     /locus_tag="VNY06_01420"
     CDS             1486..2211
                     /locus_tag="VNY06_01420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012589378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer-membrane lipoprotein carrier protein LolA"
                     /protein_id="HWX13533.1"
                     /translation="MADFMKRMAVAIAVSLALPGPAPLYADPSPAGQKNLKSASPQSK
                     AGGTSGSPTTSKPFVPLEPEAAVQKANAYFNAASNMVGDFVQISPDGTRSEGKIYVQR
                     PGKLRFEYNPPATLEVVADGLSVAVHDRKTATKDVYFISQTPLKFLLKDQIDLSRDVK
                     IIDVKSAPDSVSILVEDKATFGGTSQIRLIFDPDTFSLKQWQVTDPQGYETLVSLFNI
                     DLSKKPDPRLFQLTQERISNTNN"
     gene            2601..3416
                     /gene="xth"
                     /locus_tag="VNY06_01425"
     CDS             2601..3416
                     /gene="xth"
                     /locus_tag="VNY06_01425"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020173643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008311 - double-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HWX13534.1"
                     /translation="MRLRLATWNINSVRLRIELVARFLKDEAPDVLCLQETKCRDGEF
                     PFVPFHKLGYSHVAISGQKGYHGVAVASKLPLAQISQRFFCGNGDARHICVILAPGGT
                     QSPPITLHNFYVPAGGDEPDPGINPKFAHKLAFLEEMAEWIRLGGIAQSPAILAGDLN
                     IAPLETDVWNHKALLSVVSHTPIEVEKFWAVHAAGPWVDALRHFIPPEERLYTWWSYR
                     ARDWRKSDRGRRLDHIWVSESLKPHLDAARVLPDSRGWEKPSDHVPVIAELQF"
     gene            complement(3664..>4001)
                     /locus_tag="VNY06_01430"
     CDS             complement(3664..>4001)
                     /locus_tag="VNY06_01430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX13535.1"
                     /translation="EVRVRKARFSKPDAKTIKSLQVMLAGEPSLECWANSKDLVKPRI
                     DYFPIGLECRAPMATDKFEWVTIARKGGTSELERKVQWLFENPSMRLLVSEEECADGM
                     GPDAQQYLT"
BASE COUNT          904 a         1165 c         1032 g          900 t
ORIGIN      
        1 aatcttagct ccggtacgct gcggccacgc ccatcgagat cgagccggaa gggcaagcct
       61 tcgcaaatgt ttggcgaggt atggcaatgg acgcagagcg cctacctgcc ctatccaggc
      121 taccgcgcgc cgcaaggagc gctcggcgaa tacaatggga aattcatggt cggtcagcaa
      181 gtattgcgtg gcgcctcctg cgtgacacca gccggacata cgcgggccag ctatcgcaac
      241 ttcttttatc cccaccagcg ctggcagttt gccgggctgc gcctcgcctc ggagttctta
      301 tgatgccgaa atccaacttc gctctgcgca aaactgctcc ttggccaaat tccgaattcg
      361 caacgagcgt catgaatggc ctgtcaaagc ggtgcaaaag ccttccttgc aaatattttt
      421 atgacgcgcg gggaagtgaa ctttttgaga aaataaccaa tctgccggaa tattatccaa
      481 cgaggaccga gattgcgatc ctcgaagtgc atgcggcgga aatcgccaat ggcgtgcccg
      541 accgtgccat acttgttgaa ttcggttcag gctcaagccg caagaccgag atcttgcttg
      601 ctgcccttcc aggactttgc gcctatgttc cgatcgatgt atcagaaagc gcgcttcatg
      661 gagcaaagca gcgccttcaa aagcggtttc cgacacttga ggtgcacccg atcgtagcgg
      721 acttttcccg gtccgccacg ctaccgtcca acctcgcgtc gctccccaaa ttgggtttct
      781 ttccaggctc gacaatcggc aatttttctc ctccttccgc cgtccggttg ctccgcgcca
      841 tgcgggacat gctatcgccc gaaggacacc tgatcattgg ggtcgacctc aaaaaggagg
      901 cccggaagct cgtctgcgcc tatgatgatt cggaaggtac caccgccgcc tttaacctca
      961 atctcttagc gcggatcaac cgcgaactcg aaggtacgtt cgacctcgaa ggcttcggcc
     1021 atgaggcaat ttataatccg cgcgaggggc gcatcgagat gcgccttgtc gccaagaagg
     1081 ctcaagccgc gagcatattg ggccgacggt tccagttttt cgacggtgaa atcatccaca
     1141 cagagaattc gtacaaatat tgcactggac aatttcagga gcttgcccgc gcagcaggct
     1201 ggcatcccaa ccgcctatgg tccgatgagc agaatctctt cagcgtgcac gagctgatct
     1261 ccaaaacatc cttcggtacg gcgattcgtt gcgcgctctt caattggctg ccgggcgagc
     1321 gcggcgacag cgccgcttga aaacgcgctc actggccttg agccaaagag catgggtggg
     1381 gtaagagctt ctttgcactc atatatttga ttgcaaaggt ttagctggac ctagagtctc
     1441 caaagtcggt gcctcgaggt ttgagaacaa aaaggcacgg tgcaaatggc tgactttatg
     1501 aaacggatgg cagttgcgat agcagtctcc ctcgctttgc cggggccagc gccgctttat
     1561 gcggatcctt cgcccgccgg tcagaagaac ctaaaatcgg cttcgcccca aagcaaagct
     1621 ggcggaacct ctggtagccc cacgacatca aaaccctttg ttcctttgga gcctgaggcc
     1681 gccgtccaaa aagccaatgc ctatttcaat gccgcctcca acatggtcgg cgatttcgtt
     1741 cagatcagcc ccgacggcac caggtcggaa ggtaaaatct atgtccaaag gcctggaaag
     1801 ctgcgcttcg aatataatcc gcccgccacg ctcgaagtcg tcgccgatgg cctctccgtc
     1861 gctgtgcatg accgcaaaac cgcaacgaag gatgtttact tcattagcca aacgccgctg
     1921 aaatttctgc tcaaggatca aattgatctt agccgcgatg ttaagatcat tgatgtgaag
     1981 agcgcgcctg actccgtctc aatactggtt gaagacaagg caacatttgg cggcacctcg
     2041 caaatcaggt tgatattcga tcccgataca ttctcgctaa aacaatggca ggtgaccgac
     2101 ccgcaaggct atgaaactct tgtttcgctt ttcaatattg atttgtcgaa aaagcctgac
     2161 ccaaggctgt tccagcttac ccaggaaagg atatcgaaca caaataattg acaatgcgag
     2221 cggcgagcct ttgattgccg caggggtgca aaaaaaaaga aattttttca ataagatagg
     2281 gcaaagtgcc cactttctca aaatcaatcc ccccgccgaa cgaagcaagc tcaacctaga
     2341 tacgcgcttg aaagcgggca gttcaggttt attttaacgc ctgtgagcga cgaaactgat
     2401 caggcgcatc cccccgttcc gcctgacgcg ttgttgccgg acccctcccg gtatatctcg
     2461 ctcctagggc gcttggtatt taccaggcgc cctttttcgt tgccacgagc agccctaaca
     2521 actgcgaacc tgtgggcttc tatcctgcaa cgcttgcgtc agtacctccc gctcggatat
     2581 tctgagctgc ggagacctgc gtgcgtttga gacttgccac ttggaacatt aattccgttc
     2641 ggctgcggat cgagctcgtc gctcgcttcc tcaaagacga agcgccggac gttctctgcc
     2701 ttcaggagac aaagtgcagg gacggcgagt ttccttttgt ccccttccac aagcttggct
     2761 actcccatgt cgcgatcagc ggtcaaaagg gctatcatgg cgtggctgtc gcttcaaaac
     2821 ttccgctggc gcagatttct cagcgcttct tttgcggcaa cggcgacgcc cgccacatct
     2881 gcgtgatcct ggcacctggc gggacccaaa gcccgcctat taccttgcat aatttttacg
     2941 tccccgcggg gggcgatgag ccagatcccg gcataaatcc aaagttcgcc cataaactcg
     3001 cgtttttaga ggagatggca gagtggatcc gcttgggcgg gatcgcacag tcgccggcga
     3061 tcctggcggg cgatctcaat atcgctccgc tcgaaaccga cgtttggaat cacaaggccc
     3121 ttctctctgt cgtgagccac acgcccatcg aggtcgaaaa gttttgggcc gtgcatgcgg
     3181 ccggtccatg ggtcgatgcg ctgcgccatt tcataccccc cgaagagcgg ctctatacct
     3241 ggtggagcta tcgcgcgcgg gattggcgaa aatccgacag ggggcggcgg cttgaccata
     3301 tttgggtcag cgaaagcctc aagccgcacc tcgatgctgc gcgggtgtta cccgacagcc
     3361 gcggctggga gaagccttcg gatcacgtgc ccgtcatcgc cgaattgcaa ttttagcgat
     3421 ctcaaagaac gcagaaccgc cgcacatcat tgatccatca tgcggggtaa aacacaattg
     3481 tcgctcggca cagccgtgcc gttgcacgcg tcacaagccc ggcgtgcaaa taagccgtcg
     3541 tctcccaagc tcaaggatta gaacgaaaaa ttcagagtga ctatgaaatc cggcatgtcg
     3601 cttgcggtaa ttcgcaatct tccgcgcctt tgcggatcgc ttatctccag cccactatag
     3661 aatttaagtc aaatattgct gggcgtcagg tcccatccca tccgcgcact cttcctcgct
     3721 gactagcaag cgcattgaag gattctcgaa gagccattga acttttcgtt cgagttccga
     3781 ggtgcctccc ttgcgcgcaa tggtaaccca ttcgaatttg tctgtggcca tcggagctct
     3841 acattctagt ccaatgggaa aataatcaat cctaggtttg acaaggtctt tggaatttgc
     3901 ccagcattcc agcgaaggct cacccgccaa cataacttgc aacgacttga tggtcttggc
     3961 atctggtttc gagaaccgtg ccttgcgtac gcgcacttcc g
//
LOCUS       DATMPU010000062         3247 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_4384940,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000062 DATMPU010000000
VERSION     DATMPU010000062.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 3247)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3247)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3247
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4384940"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            55..1461
                     /gene="ahcY"
                     /locus_tag="VNY06_01435"
     CDS             55..1461
                     /gene="ahcY"
                     /locus_tag="VNY06_01435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012383599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HWX13536.1"
                     /translation="MTHTALDYAIANIDLASWGRKEINIAETEMPGLMAVRAEYKGKR
                     PLKGARIAGSLHMTIQTAVLIETLKALGADVRWASCNIYSTQDHAAAVIAATGTPVFA
                     IKGESLTDYWTYTHKIFEWSDGDTPNLILDDGGDATLLIHLGMRAEAGDTDFLSKPGN
                     EEEEILFAAIKKRLASNPGWYSRCGHAIKGISEETTTGVHRLYIMERDGKLLWPAINV
                     NDSVTKSKFDNLYGCRESLVDGVRRGTDVMMAGKVAMVAGFGDVGKGSAASLRNAGCR
                     VMISEVDPICALQAAMEGYEVTTMEDAVPRADIFVTCTGDVDVITVEHMRAMKDRAIV
                     CNIGHFDSEIQVAGLRNFKWHNVKPQVDEIEFQDGKRIILLSEGRLVNLGNATGHPSF
                     VMSASFTNQTLAQIELWTKQGRYNRKVYTLPKHLDEKVASLHLGKIGAKLTKLTPKQA
                     AYLGLPQEGPFKPEHYRY"
     gene            1795..3144
                     /gene="glmM"
                     /locus_tag="VNY06_01440"
     CDS             1795..3144
                     /gene="glmM"
                     /locus_tag="VNY06_01440"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012589818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HWX13537.1"
                     /translation="MAGKYFGTDGIRGIANASITPEIAMKVAQATGIIFRRGDYRHRA
                     VIGKDTRLSGYMIENALVSGFTSVGMDVLLLGPMPTPAVAMLTHSMRADVGVMISASH
                     NPYEDNGIKLFGPDGYKLSDELEAKIEVLMDRASMLKLSKPADLGRAMRVEGDRARYI
                     EFAKRTLSGNFSLDGLRIVVDCANGAAYKVAPEALWELGAEVVPIGVEPDGLNINRDV
                     GSTAPAALIKKVREVRADIGIALDGDADRVMIVDEAGKIVDGDQVLAAIARSWKEKGR
                     LSRPGIVATVMSNLGLERFLQGLGLSLARTPVGDRYVLEHMLAEGYNVGGEQSGHIIL
                     SDYSTTGDGLVAALQLLAIVKQRNKPVSQVCRLFEPVPQIVRSVRFKPEKPLEESAIA
                     RAAEKGRAILGQDGRLIVRPSGTEPVIRVMAEAENLDLIESVVADVCAALSALAQAA"
BASE COUNT          726 a          876 c          914 g          731 t
ORIGIN      
        1 atgtatggcc tgctatcgac gcacatgttt ttctctctgg agcaacatca tcgcatgacc
       61 cacaccgcac ttgattacgc cattgccaac atcgaccttg cctcctgggg ccgcaaagaa
      121 atcaacattg ccgaaacgga aatgccgggt cttatggcgg ttcgcgccga atataagggc
      181 aaacggccac tcaagggcgc acggatagct ggatcgcttc atatgacgat tcagaccgcc
      241 gtattgatag agacactaaa ggcgcttggc gcagatgtgc gctgggcctc ctgcaacatc
      301 tattcgactc aagatcatgc cgcggctgtg atcgccgcca cgggaacgcc cgtgtttgcc
      361 atcaagggcg aaagcctcac cgattactgg acctatacgc ataagatctt cgagtggagc
      421 gatggcgaca cgccgaacct tattctagac gacggcggcg acgccacctt gctcattcat
      481 ttgggcatgc gcgcggaagc gggcgacacg gattttctca gcaagcctgg caacgaagag
      541 gaagagatcc tgtttgccgc gattaagaaa cggctcgcct ccaatccagg ctggtatagc
      601 agatgtgggc acgccattaa ggggatcagc gaggagacga cgaccggcgt ccacaggctc
      661 tatattatgg agcgcgatgg caagctcctt tggcccgcca tcaacgtcaa tgattctgtc
      721 accaagtcga agttcgacaa tctttatggc tgccgtgagt cacttgtcga tggggttcgc
      781 cgcgggaccg acgtgatgat ggcgggcaaa gtcgcgatgg tcgcgggatt tggcgatgtc
      841 ggcaagggct ctgcggcgtc gttacgcaat gccggctgca gggtcatgat ttctgaggtc
      901 gacccgatct gcgccttgca ggcggcgatg gagggttatg aagttaccac catggaagat
      961 gccgtgccgc gtgcggatat cttcgtgact tgcaccggcg atgtcgacgt cattacggtc
     1021 gaacatatgc gtgcgatgaa ggatcgcgca attgtctgca atataggcca tttcgattcc
     1081 gagattcagg tcgctggcct gcgcaacttc aaatggcata atgtcaagcc gcaagtcgac
     1141 gaaatcgaat ttcaggacgg caaaaggatc atacttttgt cggaaggccg cctcgtcaat
     1201 ctcggcaatg cgacaggaca tccttcattc gtgatgtcgg cgtcgttcac caatcagacc
     1261 ttggcgcaaa tcgagctttg gaccaagcaa ggccggtata atcgcaaggt ctacaccttg
     1321 cccaagcatc ttgatgagaa ggtcgcttcc ttgcacctcg gcaagatagg ggccaagcta
     1381 accaaactca ctcccaaaca ggcagcctat ctcggcctgc cgcaggaagg ccctttcaag
     1441 cccgaacatt accgctatta gcattaacgc ggttcgcgag ttggcgcacg gcgccgctcg
     1501 cgagccaaaa cgccactttc atgatataca tatcaaggtc taatctttgt tatgattccg
     1561 caatgttaac aaacgcgcgc ggcgccgcgt tcaacatctg aaactcttag actgaattgg
     1621 aatgcacgca aaacgaggcc acctttcaat tttaggtcgc tcgtttaccg tgcacgtgat
     1681 aaaatacccg cattgccgct tgcgttactt ttacattccg caaaatgatg tcatttagcg
     1741 tggtccgcct agacatcgca ggtcggcgga ttgcctcagg aggttagccc atcaatggca
     1801 ggcaaatatt ttgggaccga cggtatcagg ggtatcgcga atgcgtcgat tacacccgaa
     1861 atcgccatga aggttgccca ggcgactggc attatctttc ggcgcggcga ctaccgtcac
     1921 agagctgtga tcggcaagga cacgcggctc tctggctata tgatagagaa cgccttggtc
     1981 tcggggttca cctcggttgg catggacgtt cttttgcttg ggccaatgcc aactcccgcc
     2041 gttgcgatgc tcacgcactc tatgcgggcg gatgtcggcg tcatgatctc tgcctcgcat
     2101 aatccttatg aagacaacgg gatcaaattg ttcgggcctg atggctataa attatcggac
     2161 gagcttgaag cgaaaatcga ggtgctgatg gatcgggctt ctatgctcaa attgtcaaaa
     2221 cctgctgacc ttggccgcgc catgcgggtt gaaggggacc gcgcacgtta catcgaattt
     2281 gccaagcgta ctctctcagg gaatttttcg cttgatggct tacgcatcgt cgtcgattgc
     2341 gccaatggcg ccgcttataa ggtcgcgccc gaggccttgt gggaacttgg cgccgaagtc
     2401 gtacctattg gtgtcgaacc ggatggtctt aatattaatc gcgacgtagg ctctactgcc
     2461 cccgcggctc tcatcaagaa agtgcgggag gtgcgcgccg atattggcat agccctcgac
     2521 ggtgacgccg acagggtgat gatcgtcgac gaggcaggaa aaattgttga tggcgatcaa
     2581 gtgctcgccg ccattgcacg aagctggaag gaaaaaggcc gcctgtcgcg gccgggtatt
     2641 gtcgcaacag tcatgtcgaa ccttggtctc gaacgctttc tccaagggct cggtctgtcg
     2701 ctcgcgcgga ctcccgttgg cgatcgctac gtgctcgaac atatgctcgc ggaaggatat
     2761 aatgttggcg gtgagcagtc cggacacatc attctttcgg attattccac gacgggagat
     2821 gggctcgttg ccgctttgca gcttctggca attgtcaagc agcggaataa gccggtaagc
     2881 caagtctgca ggctattcga accggtcccg cagatcgtgc gaagcgtccg tttcaagccc
     2941 gagaaacctt tggaggagtc cgctatcgcc cgtgcggctg agaaaggccg cgcgattctc
     3001 ggtcaagatg gccgccttat tgttcgtccg tccggcaccg agcccgtcat ccgggtcatg
     3061 gctgaagccg aaaacctcga tctcatagaa agtgttgttg cggatgtctg cgcggcgctt
     3121 tcggccttgg cccaagcagc gtgacatcga cttaccaacg ccgtatcagg gcggaaacat
     3181 acggaagcca gcggcgtcat gactcatcta acatattgat aatcaagggg attgcttctg
     3241 acggttt
//
LOCUS       DATMPU010000063         2999 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_2218340,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000063 DATMPU010000000
VERSION     DATMPU010000063.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 2999)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2999)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2999
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2218340"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..656
                     /locus_tag="VNY06_01445"
     CDS             <1..656
                     /locus_tag="VNY06_01445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020174169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWX13538.1"
                     /translation="VSIMPPYETYVLYEVYITPGLMAMIAFFSGMQSSLSMVYDREMG
                     NMRALLVSPFPRWFLLTSKLAAGTAVSLAQVYAFLIVAYFWEIEPPSLWGYLTVLPAL
                     VLAGLMLGALGMLLSSLIKQLENFAGIMNFIIFPMFFASSALYPLWRVRESSPWLAFV
                     CELNPFTHAVELIRFAFYERMNFPALAIVLATTIGFLVAAIMAYDPSRGILSRREGP"
     gene            complement(668..1036)
                     /locus_tag="VNY06_01450"
     CDS             complement(668..1036)
                     /locus_tag="VNY06_01450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019198108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c family protein"
                     /protein_id="HWX13539.1"
                     /translation="MAAWAGSAAGVLAGGDAAEGEKIFSLKCKVCHQIGEGAKNFVGP
                     ELNGIIGRKAGSVPDFNYSDANKSSGITWDEASLNEYLTNPKAKIPGTKMIFAGLPKE
                     SDRDNIIAYLSQFGADGKKK"
     gene            1386..2285
                     /locus_tag="VNY06_01455"
     CDS             1386..2285
                     /locus_tag="VNY06_01455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016921547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWX13540.1"
                     /translation="MQKTGTFAWELAIVTERGLAKDAGLDVVVTEVASPEAAKVALMG
                     GSADLILSDWLWVARQRNLGGRLIFFPYSTTLGALMVPAASPIQNLVGLKGQKIAVAG
                     GPLDKSWLLLQALAKQSGQDLAKEASIIYGAPALLYQKTLSGEADATLNYWNFCVALE
                     ARGYRRLLGMDEIERRLGAKGPVAMVGYVFSEEFTQNHAPVLTRFFEAAAKAKEILVQ
                     SGTDWEKIGQRIGVTEKAELALYRQTYIDGIPRRSVAQEAADARQLYRVLTKIAGPDI
                     IGNATELDEGTFYRPVEAVPNEP"
     gene            2317..>2999
                     /locus_tag="VNY06_01460"
     CDS             2317..>2999
                     /locus_tag="VNY06_01460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018266978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HWX13541.1"
                     /translation="MLILAWQLGSSFTAHNMLPAPLAVLSVIGAEARSGALFLNLAVT
                     LARVAAAFALAMVLGAALGVAMGRIRTIDRFCDPLLVVLLNLPALVVIVLAYIWLGLN
                     ELAAIVSVTLNKLPNTVVTLREGARALDKSLDEMALVFDMPLGKKIRHVVLPQLAPYF
                     AAAMRSGLSLVWKIVLVAELLGRSNGVGFEIGIAFQLFDVTRLLAYALPFVALMLAIE
                     TFFVQPFER"
BASE COUNT          584 a          863 c          818 g          734 t
ORIGIN      
        1 gcgtttcgat tatgccgccc tacgagacct atgtgcttta tgaagtttat atcacgcctg
       61 gcctcatggc gatgatcgca tttttcagcg gcatgcaatc ctcgctctcg atggtctatg
      121 atcgcgagat gggcaatatg cgcgcgcttc ttgtcagtcc cttcccgcgc tggtttcttt
      181 taacctccaa gcttgccgct ggaacggccg tgtcgctagc gcaagtttat gcgtttctaa
      241 tcgtcgctta tttttgggag atcgagccgc cgtcgctttg gggttatctc accgtgctcc
      301 cggcgctcgt tctcgcgggc cttatgctgg gggcgctcgg catgctgttg tcgtcgctga
      361 tcaagcaatt ggaaaatttc gccgggatca tgaacttcat catcttcccg atgttttttg
      421 cctcgtcagc gctctatcct ctgtggcggg tgagggagag cagtccctgg ctcgcttttg
      481 tttgcgagct taatcctttt acccacgcgg tcgaacttat ccgcttcgct ttctacgaac
      541 ggatgaactt cccggccctt gcaattgtac ttgcaacgac aattgggttt cttgtcgcag
      601 ccatcatggc ttatgacccc tcgcggggga tactttcgcg gcgcgaggga ccataacttt
      661 gcgccaatta ttttttctta ccgtcggcgc caaattgcga aagataggcg ataatgttgt
      721 cccggtcgct ttccttggga agaccagcga agatcatctt cgtacccgga atcttggcct
      781 tcggattggt caaatattca ttgagcgatg cctcgtccca ggtgatgccg gacgatttat
      841 ttgcatcgga atagttaaag tccggcacgg agcccgcttt gcgcccaata atgccgttca
      901 actctggtcc gacaaagttc ttggcacctt cgccaatctg atggcagacc ttgcatttta
      961 gcgagaagat cttttcgcct tcggcggcat ctccgccggc cagcactccc gcggccgaac
     1021 cggcccaagc agccatggct acaagacata aatagtgacg cttcatttcc ggtttccttc
     1081 cctaactaaa gtgaggggcg cttcaatgct gcctcgccca tcgcttccct accatggcca
     1141 aaaaacgaac ctctgagact cacacaaaac atgtatcctg agacgatggc aattgacaac
     1201 ccagacttgg caggaaatct catgccatgc agcacccaac gcattcgagc accacgcgac
     1261 aagaacacaa acagtgcagt ccccggcgga cctgccgtcg ctcaattctt tacgcttttt
     1321 tctgtctcgc caccgctgcc ttactcaatg gtaaagccga ggcagctgac acaatccgcg
     1381 tcgcagtgca aaaaaccggg accttcgcct gggagcttgc gattgtgact gagcgagggc
     1441 tggcaaagga tgccggtctc gacgtcgtgg tcaccgaggt agcctcgcct gaagccgcga
     1501 aggttgctct catgggcgga tcggccgacc tgatcctcag cgactggctg tgggtcgcgc
     1561 ggcagcgaaa tctgggcggc cggctgattt ttttccccta ctcgaccacg ttgggcgcgc
     1621 tgatggttcc agcggcgtca cctattcaga atttagtcgg attaaagggc cagaaaattg
     1681 ccgtcgcggg cggtcctctc gacaaaagct ggctgctgct ccaggcactc gccaaacaat
     1741 ccggccaaga cctagcgaaa gaggcgagca ttatttatgg cgcacccgcc cttctttatc
     1801 aaaaaacctt gagcggcgag gccgatgcca ctttgaacta ttggaatttc tgcgtcgcac
     1861 tcgaagcgcg gggctaccgg cggctccttg gcatggacga aatcgaacga cgcctcggcg
     1921 cgaagggacc ggtggcgatg gtgggctatg tttttagcga ggagtttacc caaaaccatg
     1981 cgcccgtgct cacgcggttt tttgaagccg cggcaaaggc caaagaaatt ctggttcaat
     2041 ccgggacgga ttgggaaaag attgggcaac ggattggggt tacagaaaag gcggaactcg
     2101 cgctctaccg gcaaacctat atagatggca ttccgcgccg ctccgtcgcc caggaagctg
     2161 ctgatgcgcg gcaactctac cgcgtcctta ccaaaatcgc aggcccggac attattggca
     2221 atgcaacgga actcgacgag ggaacattct accgacccgt ggaagcggtg cctaacgagc
     2281 cctagcgccc gagctttggg actagcgcca ctcgctttgc tgatccttgc gtggcaattg
     2341 ggttcgagct tcactgctca taacatgctg cccgcgccgc tcgccgtcct ttccgtaata
     2401 ggcgcagagg cgcgctcggg tgcgctcttt ctcaacctcg cagtaacgct tgcccgagtc
     2461 gcggctgcct ttgctctggc aatggttttg ggggccgccc tcggcgtggc catgggccgc
     2521 atcagaacaa tcgaccgatt ttgcgatcct ttgctggtcg tactcttgaa cctgccagcg
     2581 cttgtcgtca tcgtgctcgc ctatatttgg ctcggactga atgaattggc tgcgatcgtt
     2641 tccgttacct tgaataaatt gccgaatacg gtggtgacgt tgcgcgaagg cgcgcgtgcg
     2701 ctcgacaaga gtctcgatga gatggcgctt gtgttcgata tgccgctcgg caagaaaata
     2761 agacatgtcg tgctgccgca gctcgcgccc tatttcgcgg ctgcgatgcg ttcgggccta
     2821 tcgcttgtgt ggaagattgt tcttgtcgcc gaattgctcg gccgctcgaa cggtgtcggc
     2881 ttcgaaatcg gcattgcgtt ccagcttttt gacgtgacgc ggctactggc ttacgcgctt
     2941 ccctttgtcg ctctcatgct ggcgattgaa acattcttcg tgcagccgtt cgagcggca
//
LOCUS       DATMPU010000064         3089 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_978614,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000064 DATMPU010000000
VERSION     DATMPU010000064.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 3089)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3089)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3089
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_978614"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(206..712)
                     /locus_tag="VNY06_01465"
     CDS             complement(206..712)
                     /locus_tag="VNY06_01465"
                     /inference="COORDINATES: protein motif:HMM:NF017849.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF934 domain-containing protein"
                     /protein_id="HWX13542.1"
                     /translation="MPILKGNVFLADDWRYLAPSEVPPPAGKVILTATRWQELRAEGR
                     AAGSSLGLHVEPGQDLRIFLPDLVRFGLIVIAFPKFADGRGYSLARQIRTHYGYAGEL
                     RASGEVLFDQLQLLARCGFDSFEITNAATLRLLEAGRRPMFEIFYQPGLGPEILEETR
                     PWARRASE"
     gene            complement(715..1449)
                     /locus_tag="VNY06_01470"
     CDS             complement(715..1449)
                     /locus_tag="VNY06_01470"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020176411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenylyl-sulfate reductase"
                     /protein_id="HWX13543.1"
                     /translation="MAAHESALFGDSETAARLAAAFAGMDPRSLMRLVIEDLFKGKTA
                     LVSSFGADSAVLLHMAADLDKNLPVIFIDTRVLFPETLAYRDELVALLGLTNVTTIWP
                     RSDQLAREDPEDFLWAANPDRCCEIRKVLPLAEALQGYDAWISGRKRFQALSRDNLAL
                     FESEGGRIKINPLAHWTARSVLGYLETHALPRHPLVAKNYPSIGCIPCTSPVRPGEDA
                     RAGRWRGRHKVECGLHTLPLYAGADI"
     gene            complement(1436..>3089)
                     /locus_tag="VNY06_01475"
     CDS             complement(1436..>3089)
                     /locus_tag="VNY06_01475"
                     /EC_number="1.8.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020012471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050311 - sulfite reductase (ferredoxin)
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="nitrite/sulfite reductase"
                     /protein_id="HWX13544.1"
                     /translation="RYDQFDRSFVAARAAQFRDQVARRLSGELNEEQFKPLRLMNGVY
                     LQLHSYMLRIAIPYGTLSSRQMRKLAYIARRYDKGYGHFTTRQNIQFNWPALADCPDI
                     LDELAEVEMHAIQTSGNCIRNVTADHFAGAAADEIEDPRPYAEILRQWSSLHPEFSYL
                     PRKFKIAVNGAAKDRAAIRVHDIGYQIVKNAEGEVGFAVYVGGGQGRTPFIAHKIRDF
                     LPKASLLAYNNAIMRIYNLEGRRDNKFKARIKILIHEKGADTIRAEVESEFANHRDEA
                     LALPQHEIARICAYFAPPWLPARPAYSMPLERRKASDPAFAHFAAQNMVPHKTPGYGI
                     VTISLKPIGGIPGDASAESMELVADLAEQFSQDEIRVTHEQNLVLPHVALNDLPEVFD
                     GLAAQGLATANANLVTDIIACPGLDYCALATARSIPIAQRISERFGDSVRAREIGELK
                     INISGCINACGHHHVGHIGILGLEKSGAEAYQITLGGAGDESCAIGTLTGRGFSSDEV
                     VGAIETLVDTYLNLRSSPEENFVAAYRRVGMAPFKEALYGRA"
BASE COUNT          690 a          854 c          930 g          615 t
ORIGIN      
        1 atcccgaatt aattacccgg aactcgtatc agatctcatc agaaccaagg tctcttgggc
       61 cagtgtggca gggctcattc aggctagcac gacttgcaac acgaacggaa cgactaccgt
      121 tgtcgtggcg agcgctacaa atgaacggcg tctctggcca agaccttatc ctatccatgg
      181 cggtagcata gaggcacgcc gtgggtcatt ctgaggcgcg gcgcgcccaa ggtctcgtct
      241 cttccagtat ttcgggccca agtccaggct gataaaaaat ctcgaacatg ggcctgcggc
      301 ctgcctcaag aagcctgagg gtggcggcat ttgttatttc gaaactgtcg aagccgcaac
      361 gggcgagcaa ttgcagctgg tcaaacaaaa cctcgccgct tgcgcgcagc tcgcccgcat
      421 agccataatg ggtgcgaatc tggcgagcga gcgaatagcc gcggccgtcg gcgaatttgg
      481 gaaaggcaat tacgatgagg ccaaagcgga cgaggtcggg caaaaaaatt cgtaagtcct
      541 ggcccggctc aacatgcagg ccaagcgagc tgccagcggc gcgcccctct gccctcagct
      601 cctgccatct tgtcgctgta agtatgacct tgccagcagg cggcgggact tcgcttggcg
      661 ccagatagcg ccagtcatcc gccaagaata cattgccttt taaaatcggc atcgtcaaat
      721 atccgcgccg gcataaagcg gtaaggtgtg aagtccacac tcgactttat gacgcccgcg
      781 ccagcgcccc gcacgggcgt cttcgccagg ccttacgggt gaggtgcaag gtatgcatcc
      841 gattgaagga tagtttttgg caacaagcgg atggcgtggc agggcatggg tttcaaggta
      901 gcccaagacg ctgcgcgctg tccaatgtgc gagcggattg atcttgatgc ggccgccctc
      961 gctttcgaag agcgcaagat tgtcgcgcga gagtgcctga aaccgtttgc gaccgctgat
     1021 ccaagcatca tagccttgga gcgcctccgc aagcggcaag accttgcgaa tttcgcagca
     1081 acggtcgggg ttggccgccc aaagaaaatc ttccgggtct tccctggcga gctgatcgga
     1141 ccgcggccag atggtcgtga cattcgtcag gccaagaaga gcgacgagtt cgtcacgata
     1201 agcgagagtc tccggaaaca aaactcgcgt gtcaataaat ataaccggca gatttttgtc
     1261 gaggtctgcg gccatatgga gaagcacggc ggaatccgcg ccaaaactcg agacaagagc
     1321 agtctttccc ttaaacaggt cttcgatgac taagcgcatc aaggaacgcg gatccatgcc
     1381 ggcaaatgcc gcggccagcc gcgcggcggt ttcggaatcg ccaaataagg cagattcatg
     1441 cgcggccata aagagcctcc ttgaacggcg ccatgccgac ccgccgataa gcggcgacaa
     1501 aattctcttc ggggctgctc cgcagattca gataagtatc gacgagcgtt tcaatcgcgc
     1561 caacgacctc gtcagaagaa aagccgcggc cagtgagggt gccgatggcg caactttcgt
     1621 cgccggcgcc gccgagcgtg atctgatagg cttcagcacc agacttttcg agaccgagaa
     1681 tcccaatgtg gccgacatgg tgatgtccgc aggcattgat acagccggaa atattgatct
     1741 tgagctcgcc gatttcccgt gcgcggacac tatcgccaaa tcgctccgaa atgcgctgag
     1801 cgattggaat tgagcgcgcg gtcgctaggg cacaataatc gagccctggg caggcgatga
     1861 tgtcggtcac aaggttcgca tttgcggtgg caagaccctg cgcagcaagc ccgtcgaaaa
     1921 cttccggcag atcattcagc gcaacatgag gcagcacgag attctgctcg tgcgtgaccc
     1981 gaatctcgtc ttgtgaaaac tgctcagcga gatcggcgac caattccatg gactcggcgc
     2041 tggcatcgcc cggaatgccg ccgatgggtt ttaaagatat ggtgacgatc ccataaccgg
     2101 gcgtcttgtg tggtaccatg ttttgcgcgg caaagtgagc aaatgcggga tcgctcgcct
     2161 tgcgccgttc aagcggcatg gagtaggcgg ggcgcgcggg aagccacggc ggcgcgaaat
     2221 aagcgcagat gcgagcgatc tcgtgctgcg gaagggcgag cgcctcatcc cggtgattgg
     2281 caaactcgga ctcgacctcg gcgcgaatgg tgtccgcgcc tttttcgtgg atcaggattt
     2341 tgatgcgcgc cttgaattta ttgtcgcgcc gcccctcgag attatagatg cgcataatcg
     2401 cattgttgta ggcgaggaga ctggccttgg gcaaaaagtc acggatctta tgtgcgatga
     2461 acggggtgcg gccctggcct ccgccgacgt agacggcaaa gcccacctcg ccttcagcgt
     2521 tcttgacgat ctgatagccg atgtcatgca cgcggatcgc ggcacggtcc ttggcggcgc
     2581 cgttgaccgc aatcttgaac ttgcgcggca gataggaaaa ttccggatgg agcgacgacc
     2641 actgccgcag aatttcggca taggggcggg gatcttcgat ttcatcggca gctgcgccgg
     2701 cgaagtgatc ggccgtgaca ttgcggatgc aattgccgga agtttgaatt gcatgcattt
     2761 caacttcggc aagctcatcc aagatatccg ggcaatcggc aagcgcaggc cagttgaatt
     2821 gaatgttttg ccgcgtcgtg aagtggccgt agcccttgtc atagcgccgg gcgatgtagg
     2881 cgagcttgcg catttgccgc gacgacaggg tgccgtaggg aatagcgatg cgaagcatat
     2941 aggaatgaag ttgcagatag acgccattca taaggcgaag cggtttgaac tgctcctcgt
     3001 tcagttctcc tgatagccgg cgcgccacct gatcacgaaa ctgtgccgcg cgcgccgcga
     3061 caaagcttct gtcgaattga tcgtagcgg
//
LOCUS       DATMPU010000065         8273 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_3767322,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000065 DATMPU010000000
VERSION     DATMPU010000065.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 8273)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8273)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8273
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3767322"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..244
                     /locus_tag="VNY06_01480"
     CDS             <1..244
                     /locus_tag="VNY06_01480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020174793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carbamoyl phosphate synthase small subunit"
                     /protein_id="HWX13545.1"
                     /translation="VSMNHGFAVDPQTLPANARETHKSLFDGSNCGLELTDRPAFSVQ
                     YHPEASPGPRDSHYLFARFAALMEKAKTAKKAVRTG"
     gene            611..1915
                     /locus_tag="VNY06_01485"
     CDS             611..1915
                     /locus_tag="VNY06_01485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX13546.1"
                     /translation="MKHESMSVVFRSSDTPAEAAGKLLIRLGLAALFLGLPCAGIFWH
                     GAIYVLMPVGAILVLIGAFLDTLNHAGRRLGEALLTPTGATALLVGLWAALSLIWTPF
                     PAEAGVRFLQSSAAAILAGLAAIYLPQQTKTLDLYLLPAGLALTSVATIVLAYFDTPW
                     FFGGFAFDETLFERALITAIVLVWPALGLLSLRENWIAAASLAVLVAVVALAGFAQIA
                     LLAMGAGAFVFATAMSGPSKTARVLAWLMASLFILSPLLPLLYELGLRLTGVAAGPAS
                     APMLIWKDLVVSQWPRLITGHGYDFVHRGLNFGYLPAGTPTSLLFVLWYDFGLVGAAG
                     FALLLFQLFRDAGRIPASAAPAVLAGLVSVLTIAILGIATSQIWWLTLIACDAIAFAL
                     LIKGADRGRRPNVEAIRAIEAGSLKSVSAESPSVSGGSAKTS"
     gene            2170..2676
                     /locus_tag="VNY06_01490"
     CDS             2170..2676
                     /locus_tag="VNY06_01490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012590065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HWX13547.1"
                     /translation="MIETVITIRNAREGDAEGIAAVHDAAWRDAYRGLIPGRELERMI
                     TRRGPSWWQSAITRGSRLLVLDFDETIGGYTSYGRNRVPSLHYGGEIFELYIAPEFQG
                     LGFGTRLFEVARKDLAEHGYPSVLVWALADNERAVQFYLKLGGRIVRQAVERFGSETR
                     ARVAFAFH"
     gene            complement(2824..3465)
                     /gene="metW"
                     /locus_tag="VNY06_01495"
     CDS             complement(2824..3465)
                     /gene="metW"
                     /locus_tag="VNY06_01495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012383584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine biosynthesis protein MetW"
                     /protein_id="HWX13548.1"
                     /translation="MAKVDEAGRVRAFPARVDLLLVADMVEAHSRVLDVGCGDGTLLR
                     LLAEEKGVDGRGIELSQRGVNDCVAKGLSVIQGDADTDLADYPDNVFDYVILSQTLQA
                     TRQPRTVMEHMLRIGRHAIVSFPNFGHWRIRFQVAFGGRMPLTRNLSCAWYETPNIHF
                     CTIQDFIALVKEIGAHIERGVALDRFGAPLAFSEPWIWNLLGEQGVFRLARKT"
     gene            complement(3478..4668)
                     /locus_tag="VNY06_01500"
     CDS             complement(3478..4668)
                     /locus_tag="VNY06_01500"
                     /EC_number="2.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020173605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-acetyltransferase"
                     /protein_id="HWX13549.1"
                     /translation="MQFVPISKAAGIREVDAPQSLVVQFGADRPLKMEAGASLSYLTI
                     AYQTYGELNAAKSNAILICHALTGDQHVANTHPITKKAGWWPTMVGPGRPIDTSRYFV
                     IASNVVGGCMGTTGPASLNPATGRPYGLELPLVTIKDMVRAQAMLIDRLGIDSLFCVA
                     GGSMGGMQVLQWVASFPERVFAAMPIATSARHSSQNIAFHEVGRQAIMADPDWRQGRY
                     LEEGVIPTKGLAVARMAAHITYLSDEALQSKFGRKLQNRSAPTFSFDADFQIENYLRH
                     QGTSFVDRFDANSYLYVTRACDYFDLAADYGGSLAAAFKSVRTRFCVVSFNSDWLYPT
                     QASRAIVHALNAGGASVSFVDIETDRGHDAFLLDTPEFIATSHGFLDAAARARGIAPA
                     TCVS"
     gene            complement(4837..5304)
                     /gene="panD"
                     /locus_tag="VNY06_01505"
     CDS             complement(4837..5304)
                     /gene="panD"
                     /locus_tag="VNY06_01505"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012590061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HWX13550.1"
                     /translation="MRKVVGGKLHGIRVTEANLDYHGSITLDPEHCEAAGILPLEFVE
                     VWNKHSGARISTYVILGERGSRCCIVNGAAARTCQRGDELIVCSSIYLDEDRISGLSP
                     SVVTLDDDNNIVEALRYSVTLDSAGRFQFMILDESGVRREVPLRSSSGGRRRI"
     gene            complement(5508..7532)
                     /locus_tag="VNY06_01510"
     CDS             complement(5508..7532)
                     /locus_tag="VNY06_01510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015918710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl/propionyl/methylcrotonyl-CoA carboxylase
                     subunit alpha"
                     /protein_id="HWX13551.1"
                     /translation="MFSKILIANRGEIACRIIKTAKRLGIATVAVYSDADRDAMHVAL
                     ADEALRIGPPKPQESYLDGEKILKAARICGAEAIHPGYGFLAENADFAENCAEAGIAF
                     IGPPPEAIRAMGDKAQARALMEEAGVPLLPGYHGSEQDETSLAQEALHIGYPVIIKPS
                     AGGGGKGMKVVDGAADFNEKLASAKREALSAFGDDRILIEKYLMRPRHVEVQIFADRA
                     GNSVHLFDRDCSLQRRHQKIIEEGPAPKIPDELRHAMRNAAVEAVRVIGYVGAGTVEF
                     LLSSDDGAFYFLEMNTRLQVEHPVTEMITGLDLVEWQIKIAAGHALPLRQEGIVSSGH
                     AIEARLYAENPARGFLPQAGRLLHLRFPDPGPHLRVDTGVAAGDTIPVDYDPLISKII
                     VWGEDRENALLRLRGALGGIRVAGPRTNLDFLLAIAAHEAFGAAPLGTDFVERQLESL
                     LAEEKFVPPEVVAIAAIGLICERVAETHERASRTSDPWTPWSKLNGWRLNGEARETIH
                     LYEILAGGGSEHGLEVIYLRQGFRLDLQQAIFSCANARLARDGALEADLDGHRLKAVW
                     LRNGQEVLVYVGPRGPYRFEANRPAAEAARREGAAGQLTAPMPGRVAALLVAPGTLVE
                     TNQPLLVLEAMKMEHLLRAPRDGVLKKFNFQLGDPVAEGAELVTFETADG"
     gene            complement(7807..8273)
                     /locus_tag="VNY06_01515"
                     /pseudo
     CDS             complement(7807..8273)
                     /locus_tag="VNY06_01515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012631264.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
BASE COUNT         1707 a         2397 c         2366 g         1803 t
ORIGIN      
        1 cgtatcaatg aatcacgggt ttgcggtcga tccgcagacc ctgccggcga atgcgcgcga
       61 aacccacaaa tcgctgtttg atggttcaaa ttgcggtctt gaattgacgg accgccctgc
      121 cttttcggta cagtaccacc cagaagcatc gccaggccca agggatagcc attatttgtt
      181 tgcccgcttc gctgccctga tggaaaaggc gaaaacggcg aagaaggccg ttcgcaccgg
      241 gtgaggatgg tgtgcccgcg cgggacttga cccagtgagt ggtttggagc ggcataagtt
      301 tggatgaaag cctgcatgcg gtcaatacgc tagctcgtcc cccaaagcct cctcgacacc
      361 acaactccac ccaaaataga taggctatcg ccttgctgcg cggtgcgcac attttgcggt
      421 tgaatatcct gccaacgaca ggatcgcatt gagtaatatt taaaattgat agttatcact
      481 taatacaccg gcaagtttgc cctcctggct ttcacgcttt aaagttgcga attaatcgcg
      541 gcggaaaggc gtatctcatt ttttgcgcga aatgggtcta agcgaatccc acacgcccgg
      601 gcctttattc atgaaacacg agtccatgtc cgtcgtcttt cgatccagtg acacgccggc
      661 cgaggccgct gggaaacttc tgattcggct tggactggct gccttattcc tcggtctgcc
      721 ttgcgcggga attttctggc atggcgcaat ctatgtttta atgccggtgg gtgcgatctt
      781 ggttctcatc ggggcatttt tggacacctt gaaccatgcc ggccggcggc taggggaggc
      841 actccttacg cctacgggcg ccacggcttt gttagtgggc ttatgggcag ctttgtctct
      901 aatttggacc ccgttcccgg ccgaagcggg tgtacggttt ttgcagtctt ctgctgcggc
      961 tattcttgcc gggctcgccg cgatttattt gccgcagcag acgaagactc tcgatttata
     1021 tctgctacct gcgggcctcg ccctgacgag cgtggctaca atcgtcttgg catatttcga
     1081 tacgccttgg ttttttggcg gctttgcttt cgacgaaacg ctgttcgaac gcgccttgat
     1141 tactgcaatc gtcctggtat ggccagcact gggactgctc tccctgcgcg agaactggat
     1201 tgccgcggca agcctcgcgg tgctcgtggc cgtcgtggca cttgccggat ttgcccagat
     1261 cgcgctgctc gcgatgggcg ctggcgcatt cgtcttcgcc accgccatgt ccggcccgtc
     1321 caagacggca cgagtcctgg cctggctcat ggcaagctta tttatattat ctccacttct
     1381 tcctttgctt tacgagctcg gactcagatt gaccggagtt gcagcgggcc cagcttcagc
     1441 gccaatgctt atttggaagg accttgtcgt ctcgcagtgg cctcgtttga ttaccgggca
     1501 tggatatgat tttgtgcatc gagggttgaa cttcggctat ttgcccgcgg ggacaccgac
     1561 aagcttgttg ttcgttctct ggtacgattt tggactcgtg ggtgccgctg gtttcgcgct
     1621 tctgcttttt cagcttttca gagatgccgg gcgaattccg gcaagcgcgg cgcctgctgt
     1681 gctcgccggc ctcgtctcgg tcctgacgat agccattttg ggaattgcaa cgtctcagat
     1741 atggtggttg acgctcattg cctgcgacgc catcgccttc gcgctcctca tcaagggagc
     1801 agaccgaggc aggcggccga acgtcgaggc gattcgcgcg atcgaagccg ggtctctaaa
     1861 atccgtctcg gcagaaagcc cctcggtctc tggtggctcc gccaagacgt cctagcgcgt
     1921 gtcccccgca aaagtcgcgg aaccggtttt gcgtcaggac aggcgacaaa tcttgaatcc
     1981 agagcacacg atgacgtttc gaagaatggt caccttgctc cagcatcatt tatgggattt
     2041 tgatcatggg cccatcacac tatcgtgaga gcggcgaacc gtgttctttt cgcaatggcc
     2101 gcgcaacaaa tgcaagaaag cgcgaaagag ctcttgcagc ggcggcccaa gcggccttaa
     2161 cattgcaaaa tgatcgaaac ggtcattacc ataaggaatg ctcgcgaagg cgatgccgaa
     2221 ggaatcgccg cggtgcatga cgctgcttgg cgcgacgctt ataggggcct cattccgggc
     2281 cgggagctcg aacgtatgat tacgcggcgc gggccaagct ggtggcagtc agccatcact
     2341 cgggggtccc gcctgcttgt ccttgatttt gatgagacga tcggcggcta caccagttat
     2401 gggcgcaacc gcgtaccttc gctgcattat ggcggagaga tttttgagct ttatatcgcg
     2461 ccggaatttc aggggcttgg tttcggtacg cgtctttttg aagtggcgcg aaaagatctt
     2521 gccgaacatg gctatccttc cgttcttgtt tgggcgctgg ccgacaatga gcgggcggtg
     2581 caattttact tgaaacttgg gggcagaatt gtgcgccagg cggtggagcg ctttgggtcc
     2641 gaaacccgcg cccgggtggc cttcgctttt cactagttct tttgggccgc atcgtgtgcc
     2701 cgctccgcag gctgcgcgtt tcgcaaactc gatccaagcc caaagtgtcc taaactcctt
     2761 gagccgctgc ttacccgagt gccacgccgg ccaccgggca gacaaaatta gcgtcagcgg
     2821 agctcacgtt ttgcgtgcga ggcggaacac gccttgttcc ccaagcaagt tccaaatcca
     2881 gggttcgctg aaggccaatg gcgcgccaaa gcgatcaagg gcgacaccac gctcgatatg
     2941 agcgccgatc tcctttacga gggcgataaa atcctgaatg gtgcaaaaat ggatatttgg
     3001 cgtctcgtac caagcgcagg ataagttgcg tgtcaggggc atccgcccgc cgaaggcaac
     3061 ctgaaaccgg atccgccaat gaccaaaatt cggaaacgac acgatcgcat ggcggccgat
     3121 tcgcagcatg tgttccatga ccgtgcgcgg ctggcgggtc gcctgcaatg tctgtgacag
     3181 aatcacatag tcgaagacat tatccggata gtcggcaagg tccgtgtcgg cgtccccctg
     3241 aatgaccgat agccccttgg caacacagtc attgacgcct ctctgggaga gctcgatgcc
     3301 gcgtccgtcc acccctttct cttcggcgag cagccgcaac aatgtgccat ctccacagcc
     3361 aacatcgagc acgcgggagt gcgcctccac catatccgcg acaagcagca gatcgacgcg
     3421 agctgggaag gcgcgaacgc gcccagcttc gtctactttc gccaccgcgg ccatgcctca
     3481 actcacgcaa gtggctggcg caatgccacg tgcccgcgcc gccgcatcca agaacccgtg
     3541 cgatgtcgcg atgaactcgg gagtgtccaa aagaaacgca tcatggccgc ggtccgtctc
     3601 gatatcaaca aaggaaaccg aggcgccgcc agcgttcagc gcatgaacga tcgcgcggga
     3661 ggcttgggtc ggataaagcc agtccgaatt gaaggagacg acgcagaacc gcgttctcac
     3721 gctcttaaac gccgctgcca gagacccgcc ataatcggcg gcaagatcaa aataatcgca
     3781 ggcccttgtc acatagagat aggaattcgc gtcaaatcgg tcgacgaagc ttgtgccctg
     3841 gtggcgcaga tagttttcaa tctggaagtc ggcatcaaac gaaaacgtcg gcgcactgcg
     3901 attttgcagc ttgcggccaa acttgctctg aagggcttcg tccgacaaat aggtgatgtg
     3961 cgccgccatt ctggcaacgg cgagcccctt cgtcgggatg acgccctctt cgagatagcg
     4021 gccttggcgc caatcggggt ccgccatgat tgcttggcgg ccaacctcat gaaaagcaat
     4081 attttgcgag gaatgccggg cggaagttgc aattggcatt gccgcgaaga cccgttccgg
     4141 gaaactcgca acccattgca ggacctgcat cccgcccatt gaacccccgg cgacgcagaa
     4201 aagcgagtcg ataccgaggc ggtcaataag catcgcttgc gcgcgaacca tgtccttgat
     4261 cgtaacgagc gggagttcga gaccataggg ccttccggtc gcgggattaa gcgaggcagg
     4321 tcccgtcgtg cccatgcagc cgccaacaac attcgatgca atgacgaagt agcggctggt
     4381 gtcgatagga cgccccggac cgaccatagt gggccaccag ccagccttct tggtgatggg
     4441 atgcgtgttt gcgacatgct ggtcgccggt taaggcatgg cagattaaaa tggcattgga
     4501 cttagcggca ttcagctcgc cataggtctg ataggcgatg gtcaaatagc tgagcgatgc
     4561 gccagcttcc atcttcagcg gacggtctgc gccaaactgc acgacgagac tctgcggggc
     4621 gtcgacctcg cgaattcccg ctgctttgct tattggaaca aattgcacag ttctatcagc
     4681 cgttcggtat ggcgaacatt atgtgcgcca tatgggagcg cagcaaaaaa agagtcaagt
     4741 ttgcgccgcc ctgcagcggg tggcgaggcc aacttgcatc gtcttttctt cgtcgaatcg
     4801 gcaaatggcc ccgcaccagc gccgtcccgc caaatatcag attctccgcc ttccgcccga
     4861 gctcgaacgt agcggaacct cgcggcgaac gcccgattca tcgaggatca tgaattgaaa
     4921 acgccctgcg ctgtccagag taacggaata acgcaaagcc tccacaatat tattgtcatc
     4981 gtccaaggtc acgacagaag gagacaggcc cgagattcta tcttcgtcca aatagattga
     5041 gctgcagacg atgagttcgt cgccgcgctg acaggtccgc gctgcagctc cattgacaat
     5101 gcagcaccga gaaccgcgtt cacctagaat gacataggtt gagatacgcg ccccggaatg
     5161 cttgttccag acctcgacga attcgagcgg caggatgccg gcggcttcgc aatgttccgg
     5221 atcgagcgtg atcgagccgt gataatcgag attggcctcg gtgacgcgga tgccatggag
     5281 cttaccgcca acgaccttac gcatgcttag ccgtttccat aaatgggaac agaacagacg
     5341 aggcttgatt gcagcaaaca agcctagggg cttctcttta aaacatttcc tggagcagca
     5401 cgatttgccg ccgaacgtgg cggataagga aagcctctta ggcgcttgcc gcccgccgcg
     5461 ccagcctaca tttaggcagc cgaaggccaa atgtcacgcg gcaaggccta gccatctgca
     5521 gtctcgaatg tgacgagctc ggcgccctcc gcaaccgggt cgccgagttg gaaattgaac
     5581 tttttcaaga ccccatccct gggcgcgcgc agcaaatgct ccattttcat cgcctccaaa
     5641 acgagcagag gctggttggt ttcgaccaag gttccgggcg caacaagcag cgcggcgact
     5701 cgtcccggca ttggcgcggt gagttgcccc gcggcccctt cccgccgcgc cgcctcggcg
     5761 gccgggcggt tggcttcaaa ccggtaaggc cctctcggcc ccacatagac aagcacttcc
     5821 tgcccgttgc gaagccaaac cgccttcaag cggtgaccgt cgagatcggc ttcgagagcc
     5881 ccatcccttg caagccgagc gttcgcacac gaaaagattg cctgctggag atcaaggcga
     5941 aagccttggc gcaaatagat gacctcgagg ccatgctcgc tgcccccacc ggccaagatt
     6001 tcgtagagat gtatcgtttc gcgcgcctcg ccattgagcc gccagccatt tagcttgctc
     6061 cacggggtcc aagggtcgga ggttcggctc gctctttcgt gcgtttccgc tacccgctcg
     6121 cagatgaggc caatggccgc gattgcaaca acctctggcg gcacaaactt ttcttccgcc
     6181 aggaggcttt caagctggcg ttcgacaaag tcggtaccca gtggggcggc accaaaggct
     6241 tcatgcgcag caatggcgag caaaaaatcg agattcgttc gcggccctgc tacacgtatc
     6301 ccgcccaacg caccgcgcag acgcaagaga gcattctcgc gatcttctcc ccaaacgatt
     6361 attttggaaa tcagcggatc gtaatcaaca ggaattgtat ctcctgccgc aacgccggta
     6421 tcgacacgaa gatgcggccc cgggtccgga aatctcagat gcaagagacg gcctgcttgt
     6481 ggcaagaaac ctcgcgccgg attctcggca taaagccgtg cctcaatcgc atggccgctt
     6541 gaaacgatcc cttcttggcg cagagggagg gcgtggcccg cagcaatttt gatctgccat
     6601 tcgacgagat cgagaccggt gatcatttcg gtcaccggat gctcgacctg gagccgggtg
     6661 ttcatttcga gaaagtagaa ggcgccatca tcagacgaca gcaaaaattc gactgtaccg
     6721 gcgcccacgt agccaattac gcgcacggct tcgaccgccg cgttgcgcat cgcgtggcgc
     6781 agctcgtcgg ggattttagg ggcaggccct tcctcgatga ttttttggtg cctgcgctga
     6841 agtgaacaat cgcggtcgaa tagatggaca gaatttcccg ccctatcggc gaagatttgc
     6901 acttccacat gacgcgggcg catcagatat ttttcaatta aaatgcggtc atcgccgaaa
     6961 gccgacagtg cttcgcgttt ggcgctcgca agtttttcat taaagtccgc tgctccatcg
     7021 acgactttca tgcccttgcc cccgccaccc gccgacggtt ttattatgac gggatagcca
     7081 atgtgcaggg cttcttgcgc cagcgaggtt tcgtcttgtt cgctgccatg atagcctggc
     7141 aagagaggaa cgcccgcctc ttccatgagg gctctggcct gggctttgtc ccccatcgct
     7201 ctgatggcct ctggaggcgg accgatgaag gcaatgcccg cctcggcgca attttcggcg
     7261 aaatccgcgt tctcggccaa aaacccatat cctggatgaa tggcctctgc cccgcaaatt
     7321 cgcgcggcct tcaaaatttt ctcgccgtca agatagctct cttggggttt tggtggccct
     7381 atccgcaaag cctcgtcggc aagcgcgaca tgcatggcat cgcgatccgc gtcggaataa
     7441 accgcaactg tcgcaatgcc gagccgcttt gcggttttta tgatccggca ggcaatctcg
     7501 ccgcgattgg ctatcaggat tttggagaac atcacggcgg tcccgagtcg gcacgagttc
     7561 ttgtattctg accacaaatg actgcctaaa gccagacctc cacaaaacgt ctagggggat
     7621 cagtttgaat ttctgctcat gaccgatttc gttgatttag tcgctgagcg aacgtgattt
     7681 gggtgtcgtc cgcgttctgt cgtgctggtt gagggtaatt ttctggacag gtcgctcata
     7741 aacccagtga ttggttcggg cgagcgctcg cgctgtccgc gaggcgaatg ccgtcggcgt
     7801 cgaccgaagc ttcgccagct ttcgtaaatt ctgagcggtg gcggcgagca agaactcgtc
     7861 tctcgcgccg gcgggtccac gcaggcgcag acgaccaagc ctgaggatgc gtttgagatg
     7921 ggcgaagagc atcgcgattt tcttgcccag atgtcgcgat tgttcgaagg caggcgtgct
     7981 ggcgagggtt cgagcaaaat cgcgggcatc ctcatcaagg tcgcgtggaa ccttacgatg
     8041 tggcgtgttg gggcagcagc gcggcttcag ggcgcatgcc tggcaatctg gcttgctggc
     8101 gcggtagagc cgcgtcccat ccttagtgac gcccgatctc ggggttttga aactccgacg
     8161 aaactgcacc aggagcttgc cttgcgggca agtgtcgtga ttgcgctccg gatcgaagac
     8221 aaagtcggat cgcgagaacg tgccgtccgt tcggttcgac ttgtcgaaca ccg
//
LOCUS       DATMPU010000066         6661 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_981137,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000066 DATMPU010000000
VERSION     DATMPU010000066.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 6661)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6661)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6661
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_981137"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..612)
                     /gene="opgC"
                     /locus_tag="VNY06_01520"
     CDS             complement(<1..612)
                     /gene="opgC"
                     /locus_tag="VNY06_01520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012591231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OpgC domain-containing protein"
                     /protein_id="HWX13552.1"
                     /translation="MRAPNEIDFWRGFALVTIFVNHIPGLYYERFTFRNVSLSDSAEL
                     FVFLAGWTMRKLIDGPADALPAKWLVLRLESRAFQIYIAQLVVTEFAIAMLAAAALYL
                     DAPFLLDWHNASAIFNDPVEAHLGLILLTHQLGYFNILPLYVVLVFIGPAVALLHRAA
                     TVLLLLVSVTVYAAALVLGLNLPTWPVEGTWFFNPLAWQLIYVL"
     gene            813..1364
                     /locus_tag="VNY06_01525"
     CDS             813..1364
                     /locus_tag="VNY06_01525"
                     /inference="COORDINATES: protein motif:HMM:NF013324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="paraslipin"
                     /protein_id="HWX13553.1"
                     /translation="MGAFVFLGGLIAIGLAIVDHLHRLSLPIGFVYSPLFWFGYVAIL
                     TTATVVKFVRQQEVLVIERLGRYNRTLTAGVNIVWPIVERVAYVFDLREQVMDVPAQD
                     AITKDNATVTIDGILYYKIVHARDAAYGAQDIRRAIINLAQTTMRSAIGSMELDKTFE
                     QRAEINDKVVKDVAEAAQSWALK"
     gene            1361..1855
                     /locus_tag="VNY06_01530"
     CDS             1361..1855
                     /locus_tag="VNY06_01530"
                     /inference="COORDINATES: protein motif:HMM:NF027525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HWX13554.1"
                     /translation="MTRYEIKDIKMPRSLLESMEPQMKAERDKRAVVLESEGVKQSQI
                     NRADGERQAAILRSEGEQQAAINVAEGQAEAIRLVRDQIKQDGGSEAVQLEVAKSALE
                     QYGKLAKQGNSLIIMGDNADPSGWLAKAMAVLKTAGTSPSPSSPSPVPPWQIRERGQD
                     GRDH"
     gene            1839..2330
                     /locus_tag="VNY06_01535"
     CDS             1839..2330
                     /locus_tag="VNY06_01535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004448234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HWX13555.1"
                     /translation="MDGIIDNPEILFLIAGFVLFGIDIFIIGLSPLMFVATGALVTSG
                     LLYLSGWQGGILHLTGWNPGFLEALAICAVISFLIAMVGRRPLQVFQNSNVQEDRSSD
                     LIGRELITTQDVTKTGGWVHWSGTQWQARLADSVQVERVGPGVRMQVVQVKNLALILV
                     PVA"
     gene            complement(2641..3483)
                     /locus_tag="VNY06_01540"
     CDS             complement(2641..3483)
                     /locus_tag="VNY06_01540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012115556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HWX13556.1"
                     /translation="MTKVWQDGRIVGLTEAHISVADRGFLLGDGLFETMPVHRGRLFD
                     LDAHLARLASGLVLLNLAGAVDLAKLRAGIADYLAAEDLASAVLRLTITRGYGSRGLL
                     PPEAPCPTIVMTLSSLPPARKEPLSLHVATGTRRNECSPLSRIKALPYLDNMLALQEA
                     RAHGADEAILLNTRGNIACASVANVFAVREGRLETPPLSDGALPGTMRALVLSLAKDL
                     GLVPVDRSLNVEDLAAADEVFITNSISRVMTVTECNGKPLGDRAGSALARLRALIAMR
                     FDAA"
     gene            complement(3480..4043)
                     /locus_tag="VNY06_01545"
     CDS             complement(3480..4043)
                     /locus_tag="VNY06_01545"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018386979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HWX13557.1"
                     /translation="MILVIDNYDSFVYTVANYLQELGEAPEVVRNDAELPQEIPEAIV
                     ISPGPCTPNEAGISMRLVSQHSGRVPILGICLGHQCIGEVFGGRVTRAARPMHGEASE
                     VRHEASHILHGLPNPLRVGRYHSLIVRLDASDGPLAATAWSEENEIMALEHRAHPTFG
                     VQFHPESILTEHGREMLRNFLKHIGRR"
     gene            complement(4040..5407)
                     /gene="pabB"
                     /locus_tag="VNY06_01550"
     CDS             complement(4040..5407)
                     /gene="pabB"
                     /locus_tag="VNY06_01550"
                     /EC_number="2.6.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012591397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008153 - para-aminobenzoic acid
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component I"
                     /protein_id="HWX13558.1"
                     /translation="MHVIEIDFSDPIAAADALRGLPFLTFLDSAVTDERLGRYSFVVA
                     DPFARLEFVKAAPDWVARLKALLGHYHTATLPGLPPFQGGAAGLFSYDLGRSLERLPE
                     PTFDPLAYPDLSLGLYDVTVAFDLVARRAFIISTGFPETEPRLREWRAVERASVFEAL
                     LRHGGHSLPQAGLISLEGWTSNFTREGYEKVVREVIDRILAGDIFQANLAQRFEAPVP
                     LGFDHFGFYRRLRKLNPAPFSAYLDRGDFFIASASPERFLRVEGDRVETRPIKGTRAR
                     FVDGLLDALQSRALTESRKDRAENIMIVDLLRNDLSKVCVPGSVQVPQLCALETYASV
                     HHLVSTVTGRLRPGQGPVDLLAAAFPGGSVTGAPKVRAMEIIIELERYARGPYCGAIG
                     YIGFDGTMDTNIVIRTASFRGGSCVVQAGGGIVTASEPAAEYEETLDKARAIFAAFGA
                     KELAS"
     gene            complement(6466..>6661)
                     /locus_tag="VNY06_01555"
     CDS             complement(6466..>6661)
                     /locus_tag="VNY06_01555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX13559.1"
                     /translation="LPSILAALIFSPRGFDTPRFMRHIAASINMAHGYVMLYEVARRN
                     RILLKMQNPRVCARPYVIDG"
BASE COUNT         1601 a         1859 c         1882 g         1319 t
ORIGIN      
        1 aaggacatag atgagctgcc atgcgagcgg attaaaaaac caagttccct caaccggcca
       61 ggtcggcaaa tttaaaccta gaacgagggc agccgcgtag actgtgaccg aaaccagcag
      121 cagtaggacc gttgcggcgc ggtgaagcag ggcgaccgct ggaccaatga aaactaagac
      181 aacataaagt ggaaggatgt tgaaatagcc gagctggtgt gtaagcagaa tgagacctag
      241 atgcgcctca actggatcgt tgaagatggc tgaagcgttg tgccaatcga ggaggaacgg
      301 cgcatcaagg tatagcgccg ccgccgcaag catcgcgatt gcaaactcgg taactacgag
      361 ctgagcgata tagatttgga aagcgcgaga ctctagccgc aacaccagcc atttcgctgg
      421 caacgcatcg gccgggccgt caataagttt gcgcatggtc cagccagcaa gaaacacaaa
      481 cagctccgcc gagtccgaca gtgaaacgtt gcgaaaggtg aaacgctcgt aataaagtcc
      541 cgggatatga ttgacaaaaa tcgtaaccag ggcaaaaccg cgccagaaat cgatctcatt
      601 gggtgcgcgc atggtctctc ccaaagccct ctcgcaccgc ccctaggccc tcagagcggt
      661 tcaatgtgca gcgctactcc attagcccaa attagagcct cgcaatgccg caacaataga
      721 cgccgtatta ccgaattgtg atttcggatt agaagatttt caatttgtac tgccgaagaa
      781 ttgaactcac cagaagcaaa gggagtaaaa ttatgggcgc tttcgttttc ctcggtggac
      841 tcatcgccat tggcttggcg atagtcgatc acctccatcg gctcagttta ccaatcggct
      901 tcgtctacag cccgctattc tggttcggtt acgttgccat tttgacaact gcaaccgtcg
      961 tcaagtttgt gcgccagcaa gaagtcctcg tgatcgaacg acttggacgc tataaccgga
     1021 cactgacagc cggggtcaac atcgtatggc cgatcgtcga gcgtgtggct tacgtgttcg
     1081 acctgcgcga gcaggtcatg gacgttcccg cgcaggacgc cataaccaaa gacaatgcga
     1141 cggttacgat tgacggcata ctttactaca agattgtcca cgcccgggat gccgcatatg
     1201 gcgctcaaga tatccgccgt gcgatcatca atcttgcaca aacgaccatg cgctccgcaa
     1261 ttggttcgat ggagctcgac aagaccttcg agcagcgggc cgagatcaac gacaaagtcg
     1321 tcaaggatgt cgccgaagcc gcccagtctt gggcgctcaa gtgacccgtt acgagatcaa
     1381 ggatatcaag atgccgcggt cgctgcttga atccatggag ccgcagatga aagctgagcg
     1441 cgacaaacgt gccgtggttc tcgaatcgga aggtgtcaaa cagtctcaga tcaaccgtgc
     1501 agatggagaa cgccaagcag ccatactacg gtcagaaggc gaacagcaag cggcaatcaa
     1561 tgtcgctgaa ggccaggccg aagccatcag actggttcgc gaccaaatca agcaggatgg
     1621 tggctccgaa gctgtccagc ttgaggtcgc aaaatcggcg cttgagcaat atggaaagct
     1681 cgcgaagcaa ggcaattccc ttatcatcat gggcgataac gctgacccga gcggctggct
     1741 cgctaaggcg atggcggttc tcaaaaccgc tggcacctct ccatctccgt cttccccaag
     1801 tcctgttccg ccgtggcaaa ttagagagag aggacaagat ggacgggatc attgataatc
     1861 cagagatact ttttcttatt gccggtttcg tgctttttgg catcgatata ttcatcattg
     1921 gtctttcacc cttgatgttc gtcgcaacgg gagcgcttgt gacgagcgga ctcctctacc
     1981 tctctgggtg gcagggcggc attttgcacc ttacggggtg gaatcccggt tttcttgaag
     2041 cacttgcaat atgcgccgta attagctttc tgattgcaat ggtcggaagg cggccgcttc
     2101 aggtgttcca gaactccaat gtgcaggaag atcgcagcag cgacctcatc ggacgcgaat
     2161 tgattacaac gcaagatgta accaagacgg gtggctgggt gcattggtct ggaacgcagt
     2221 ggcaggcccg cttggccgac agcgtccaag ttgaacgcgt cggccctggt gtgcggatgc
     2281 aagtggtgca agttaagaac cttgcgctta tacttgttcc ggtcgcctga atgcgtggcg
     2341 cccttatgaa atgctgctaa gagcgtgtcg agccaaaagt gggaaccggt tttgcgtccg
     2401 gacacgcggc aaagatttag agcaagatga cgattcgaag gaaagtcatc ttgctctgga
     2461 tcgcgaggcc acctccaaag cataaatcct gaccctttac ctgccgccta gtccagacct
     2521 gcttatggcc gttggaggtc agtgaattgg cgccgcccca gtattgtaaa tcgagccgga
     2581 aaagcaagtt ccggcttcct atcatcgcgg tcctgagagc cctgccccgt tgcttgcgta
     2641 tcacgctgcg tcgaacctca ttgcaatgag cgcgcgcagc cgtgccaagg cgctgccagc
     2701 gcgatcgcca agcggcttgc cgttgcattc cgtgaccgtc atgacccggc taatactgtt
     2761 ggtaataaaa acctcatccg ccgcggcaag gtcctcaaca ttaagggagc ggtcgaccgg
     2821 gaccaatccc aaatcctttg cgagcgatag cacaagggct cgcatggtgc ctggcaaggc
     2881 gccgtcggat agcggaggcg tttccaggcg cccttcccga actgcgaaaa cattggcgac
     2941 ggaggcgcaa gcaatgtttc cacgtgtatt gagcaatatt gcttcatcgg caccatgagc
     3001 ccgggcctcc tggagtgcca gcatattgtc taagtagggc agtgccttga tccgcgagag
     3061 aggcgagcac tcgttgcgcc gggttcccgt cgcaacatga agcgacagag gttctttccg
     3121 tgcgggcggc agggacgaca acgtcatcac gattgtagga caaggcgctt caggcggaag
     3181 gaggccacgc gatccgtatc ccctggttat ggtcagccgc aagacggccg acgccaaatc
     3241 ctcggccgcc agataatccg caattccagc gcgtagtttg gcgagatcga cagccccggc
     3301 taagttcaac aagacaagtc ctgaggcgag ccgcgcgaga tgcgcgtcga gatcaaataa
     3361 ccgcccacga tgaactggca tcgtctcgaa taagccgtcg cccagcaaaa acccacgatc
     3421 ggcgacggaa atatgcgcct ccgtcagacc gacaatccgg ccatcctgcc aaaccttagt
     3481 catcgccgtc cgatgtgctt tagaaaattg cgcagcattt cgcggccgtg ttccgtaagg
     3541 attgattcag gatggaactg cactccaaaa gtcggatgcg cgcgatgttc cagtgccatg
     3601 atctcgttct cctcggacca agccgttgcc gcaagcggac catcgcttgc gtcgagcctc
     3661 acgatcaagg agtgataccg cccgacgcgc aagggattgg gcaagccgtg caaaatatgc
     3721 gaagcttcat ggcgcacttc gcttgcctcg ccatgcattg gacgggcggc ccgtgtaaca
     3781 cgcccgccaa atacctcacc gatacattgg tgccccaggc aaataccgag aattggcaca
     3841 cgccctgaat gttgcgaaac aagccgcatc gatattcccg cctcatttgg cgtgcagggg
     3901 ccgggagaaa tgactatggc ttcgggtatt tcttgcggga gttcggcatc attgcgcacg
     3961 acttccggcg cttcccccaa ttcctggaga taatttgcga ccgtgtagac aaaggagtca
     4021 taattgtcga tgacgagaat cacgaagcca attccttagc gccgaaagca gcgaaaattg
     4081 ctcttgcctt atccaaagtc tcttcatatt ctgcggcagg ttcggacgca gtgactatcc
     4141 cgcctccggc ctgcaccacg cagctgccgc cgcgaaagct cgcggttctg atgacgatat
     4201 tcgtatccat tgtgccatcg aaaccgatgt agccgatcgc gccgcaatat gggcctcggg
     4261 cataacgctc cagctcgatt atgatttcca tcgcccgaac tttgggcgca cccgtgaccg
     4321 agcccccagg aaaagctgcg gccaataggt cgaccggccc ctggcctggt ctcagacgtc
     4381 cggtgacagt cgaaaccaaa tggtgaaccg atgcataagt ctccagggcg caaagttgtg
     4441 gcacttgcac cgagccagga acacaaacct ttgaaagatc gttgcgaagc agatcaacga
     4501 tcataatatt ctcggcccta tccttgcggc tttcggtgag tgcgcgggac tgcagggcgt
     4561 cgaggagtcc atcgacgaag cgggcccgcg tgcccttgat aggccgggtt tcaacgcggt
     4621 caccttcgac tcttagaaat ctctctgggg aggctgaggc aataaaaaaa tctccacggt
     4681 cgagataggc agaaaagggc gccggattga gtttgcgtaa acggcggtag aagccaaaat
     4741 gatcgaagcc aagaggcacc ggcgcttcaa atctttgcgc caagttggcc tgaaaaatat
     4801 cgcccgcgag aatccggtca atgacctcgc gaaccacctt ttcataccct tcccgcgtaa
     4861 aattgctggt ccagccttcg agtgatatca gtcccgcttg cggcaacgaa tggccgccgt
     4921 gtcgaagcaa cgcctcgaaa acgctggcgc gctcaaccgc gcgccattcg cgcagccgcg
     4981 gttctgtctc cggaaatccg gttgagatga tgaatgcacg tcgcgccacc agatcgaacg
     5041 caacggtcac atcataaagt ccaagcgaaa gatcaggata agcaagcgga tcaaatgtgg
     5101 gctcgggaag acgctcgaga ctgcggccga ggtcataaga aaatagccct gcggctccgc
     5161 cttgaaacgg gggcagtcct ggcaaagttg ccgtatgata atggcccaag agagctttta
     5221 ggcgcgccac ccagtccggg gccgctttta caaactccaa acgggcgaag ggatcggcca
     5281 caacgaaact gtagcgtccg agcctctcat cggtcaccgc actgtcgaga aaggtcagaa
     5341 acggcaggcc gcgaagcgcg tccgcagccg ctatgggatc actaaaatca atttcgatga
     5401 catgcatcgc gttgccttcc cgccgtcagg aagcccaaaa cttaagtcga tctcacccat
     5461 cgcctgtcgc gaagctaaaa aaacgggccc gatgagccgg atgcgaggag tgcattgccc
     5521 atttgagggg ataaggcaag cgggcttctt gaccgacgcg attgatctat gcggcggcgc
     5581 ccaaccgcgg catcttggcc acgtcatttg agcattggag ccattttttg gaaccgcaaa
     5641 ggaaccaggg aacgcccgtg atgttcgcgc ggagttgggc gtttacctgc ggtcacggaa
     5701 atgaagggaa gcccatatca gggtgcgaag gatcccgaac acgacggccg cgtaaaggaa
     5761 aaccgagact cgggaagcag caaaagctcc gccgctccgg cagcggtcat cagacggaaa
     5821 gttcgggaca tactccgcgc gcagagcgcg acccagagca tgacgggcgc ccaaaggaaa
     5881 accatgagaa aggcgcgcat aagagcgacg cctaagagca gcaatcaacg gtttccgggg
     5941 cgctgttagc ccggcgttct atttgttagc gcagtgcgtt ctctatatgc ccctcaaggg
     6001 gaggcatatg atccaggcct tcgccaaagc aagaaattat gccgcgcagc cgcatcggtg
     6061 cgcagggagt tcgcgggatt cctggacgag ggaagcgtca aaggcagtgg cacggcgacc
     6121 tgttccttct cacacccgca cggcacgccc gagtgttcaa tctttcttcg ccggaatttg
     6181 cggtttcggc gttgcgcgat ccggctcgct ctgcgcaggc tgttgcgacg gaggttttgg
     6241 cgagtctggc gccttcggag gctccgccgg ccgattcgcg gggcggcctt tatacgcaaa
     6301 taaaggggcg gcagcccgcc aggcgccgcg aaaactcacc cgcggccaga tggcggcggt
     6361 ttgcgctgga gcagattcgc aaatcatttg ttgattaaac atcgagtcct catcgcacgt
     6421 tccagctact gttttaaagc ggcctaggcg tattctgtgt ccgggtcaac cgtcaatgac
     6481 ataaggccgc gcgcaaaccc ttggattctg cattttcaat aaaattcggt tccgccttgc
     6541 aacctcgtaa agcatgacgt agccgtgagc catattgatg ctggcagcga tatgcctcat
     6601 aaaccgaggg gtatcgaaac cacggggaga aaatatgagc gcggcgagga ttgacggcaa
     6661 g
//
LOCUS       DATMPU010000067         5073 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_2531264,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000067 DATMPU010000000
VERSION     DATMPU010000067.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 5073)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5073)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5073
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2531264"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            103..882
                     /locus_tag="VNY06_01560"
     CDS             103..882
                     /locus_tag="VNY06_01560"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006467970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008177 - succinate dehydrogenase
                     (ubiquinone) activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit"
                     /protein_id="HWX13560.1"
                     /translation="MVQFSLPANSRVQTGKTWPRQGEANNLVEFRVYRWNPDDNQNPH
                     IDTYFVDQSDCGPMVLDALIWIKSKIDPALTFRRSCREGICGSCAMNIDGTNTLACTQ
                     YTSAIKGPVKIYPLPHLPVVKDLVPDLSVFYAQHASIEPWLQTATPPPQKEWIQTPQD
                     REKLDGLYECILCACCSTSCPSYWWNGDRYLGPAVLLQAYRWLVDSRDEAAGERLDNV
                     EDPFRLYRCHTILNCAKACPKGLNPAKAIAEIKNLMIARQI"
     gene            948..1526
                     /locus_tag="VNY06_01565"
     CDS             948..1526
                     /locus_tag="VNY06_01565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019198448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HWX13561.1"
                     /translation="MLRFLSCAAAVLLTGALCTAFAAERPATYDEMIARYATAHGVPA
                     AFVHHIVMRESRYNPRLVHHHCYGLMQIKHATARSMGYRGDARGLLDPKTNLTYAVPY
                     LANAYRIAAGNEARATALFSGGYYYAAKRKKMLGELRTASAPALTPESAQPQAFEPAR
                     SSGLLSFLGGTPGAATPERSPQSPSNLFSAVK"
     gene            1714..2004
                     /gene="rpmB"
                     /locus_tag="VNY06_01570"
     CDS             1714..2004
                     /gene="rpmB"
                     /locus_tag="VNY06_01570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012333447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HWX13562.1"
                     /translation="MSRRCELTGKAVLSGNKVSHSNRKTRTRFLPNLCNVTLISDVLQ
                     RRVRMRVAAAALRSVEHCGGLDAFLTKASDTTLSPGALALKREIAKKQAAAS"
     gene            complement(2151..4490)
                     /locus_tag="VNY06_01575"
     CDS             complement(2151..4490)
                     /locus_tag="VNY06_01575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012589637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HWX13563.1"
                     /translation="MTNVFALGTTDASAPISLRARAVALRYLDGLNREQLAAIETIDG
                     PVLVLAGAGTGKTRVLTTRIAHILAEGRARAQEILAVTFTNKAAREMQARLGTLAGPV
                     AEAMAWLGTFHAISTKILRRHAELVGLKSSFTILDTDDQIRLLKQVLQAENIDEKRWP
                     ARSLANQIDAWKNRGLDPAHVPAGEAAAFANGKGSALYRLYQERLKTLNAADFGDLLL
                     ESIRLFRDNPDILKLFHERFRYILVDEYQDTNTAQYLWLRLLAQGRQNLCCVGDDDQS
                     IYGWRGAEVDNILRFESDFPGAKIIRLERNYRSTGHILAAASGLIAHNEGRLGKTLFT
                     DGALGEKPAVTGLWDSEEEARVTGEEIEELQRKGHVLDEIAILVRASFQMREFEERFL
                     AIGLPYRVIGGPRFYERAEIRDALGFLRCVAQPADDLAFERIFNLPKRGLGEATLQLL
                     HEHARRVRVPLAQAAAAIVETEELKPKQRQALRELIGDLSRWSQLIEAKPQGELAEII
                     LEESGYIGMWRKERTADATGRLENLKELVRSMEEFPDLGAFLEHVSLVMDAANTQSDA
                     RVSIMTLHAAKGLEFESVFLPGWEEGLFPHQRSLDDSGRAGLEEERRLAYVGLTRAKR
                     RAKISFATNRRIHGLWQMTIPSRFLDELPPDHVAVVEAATGAAYGGYAQSRFANMDNY
                     ASSYATPGWQRARQRADGPGPGGSWRNQEGRAGRAPAQIEGELVARSSAGSAFAKGAR
                     VFHTKFGPGTVAITDGNKLTVDFDKAGRKMVLESFVEAG"
BASE COUNT         1123 a         1545 c         1367 g         1038 t
ORIGIN      
        1 ggtctcgatt gattaccgcc cagtgcatag tttcacgatg accaatgaaa tgcactatat
       61 tgagcccaaa aagcgcgtgt attgacaaag gctgacaaac agatggtcca attctcactc
      121 cccgccaact cacgcgtcca aaccggcaag acctggccgc gccagggcga ggccaataac
      181 ctcgtagaat tcagggttta tcgctggaac ccggacgaca atcaaaatcc gcatatcgac
      241 acctactttg tcgatcaaag tgattgcggt ccgatggttc tcgacgcgct gatctggatc
      301 aaatcgaaga tcgatccggc gctcaccttc cgccggtcct gccgggaagg catttgcggg
      361 tcctgcgcga tgaatatcga cggcacgaat acccttgcct gcacccaata cacgagcgca
      421 atcaaaggcc cggtcaaaat ttatcccttg ccgcatttgc cagtggttaa ggacctcgtg
      481 ccggatctct ccgtctttta cgcccagcac gcctctatcg agccttggct gcaaacggcg
      541 actccgcctc cccagaagga atggattcaa acgccgcagg accgcgaaaa actcgacggg
      601 ctttacgaat gtatcctttg cgcttgctgc tcgacctctt gtcccagcta ttggtggaac
      661 ggcgatcgct atcttggacc cgccgttctg ttgcaagcct accgctggct cgtcgattcg
      721 cgggatgagg cggcgggcga gcggctcgac aatgtcgagg acccttttcg tctctaccgc
      781 tgccacacga tcctcaattg cgccaaggcc tgccccaaag gcctcaaccc ggcaaaggca
      841 atcgccgaaa ttaagaatct tatgatcgcc cggcaaatct gacacaagcg ccgcgccttt
      901 ctgcggcctc aatccgtgtg cacatctagc ggggggaggc ctctttgatg ttgcgctttt
      961 tgtcttgcgc cgccgccgtg ctgctgaccg gcgcgctttg cactgccttt gcggccgagc
     1021 gtccagcaac atatgacgag atgatcgccc ggtatgcgac ggcgcatggc gtacctgcgg
     1081 cctttgtgca tcacattgtc atgcgcgaaa gccgttataa tccacgcctt gtccatcatc
     1141 attgctacgg gttaatgcaa atcaagcatg cgaccgcccg cagcatgggt tatagaggcg
     1201 acgcgcgcgg gcttctcgat cctaaaacca atctcaccta cgcagttccc tacctcgcca
     1261 atgcttaccg gatcgcggcc ggcaatgagg cgcgcgccac cgcgctcttt tctggcgggt
     1321 attattacgc cgcgaaacgg aaaaagatgc ttggcgaatt acggaccgcg tccgcacctg
     1381 ctctgacacc ggaatccgcg cagccacagg cgttcgagcc ggcccgaagc tcggggctgc
     1441 tctcctttct tggaggaacg ccgggagcgg caacgccgga gcgttcgccg caatctccga
     1501 gtaacctttt ttccgccgtc aaatgaagct taagaatcaa taagatcccc atcaagagaa
     1561 tggcattttg actggcatct ggacctaccc ttgacgccgc aaggggccta gcctaagaga
     1621 cggctccttc atcacgcgct aggattacgc gcgccggcca aacttgagtg gcgggcccca
     1681 aacagtttta gtagccgaac gacaggaata gcaatgtcac gccgttgcga attaaccggc
     1741 aaagccgtgc taagcggcaa taaggttagc cactcgaacc gcaagacgcg gacacgattt
     1801 ttgccaaatc tctgtaatgt gaccctgatt tccgatgtct tgcagcggcg agtccgcatg
     1861 cgcgtggcag ccgccgctct gcgctccgtg gaacattgcg gcggccttga tgcctttttg
     1921 accaaggcga gcgacaccac gctttccccc ggtgcattgg cgttaaagcg ggaaatcgca
     1981 aaaaaacagg cagcggcgag ttaagcaact ttccggttgg actttggaaa acgccggtac
     2041 cgcaagagac gggcgttacg ctgctgccaa cccgacgccg cccgcctttc tgtggccagc
     2101 agactcaagt caccggcatt gcgttaacga acgcggccat aaaggcaaaa tcatcctgcc
     2161 tcgacaaaac tctccagcac cattttgcga cccgccttat cgaaatcaac cgtcagcttg
     2221 ttgccgtcgg tgatagcgac cgtgccgggg ccaaatttcg tgtggaacac acgcgcgcct
     2281 ttggcaaaag cagaacccgc gctcgagcgc gcgaccagct ccccttcgat ttgcgccgga
     2341 gcacgtcccg cccgaccctc ttgattgcgc caggacccgc ccggccccgg cccgtccgcg
     2401 cgctgcctgg cgcgctgcca gcccggcgtc gcataggagg acgcatagtt gtccatattg
     2461 gcaaaccgcg attgcgcata gccgccataa gcagcccccg ttgccgcttc aacaaccgcg
     2521 acatggtccg gcggtaattc atcaaggaaa cgcgatggaa tggtcatctg ccacagcccg
     2581 tgaatgcgcc ggttggttgc aaaactaatc ttcgcgcggc gcttggcgcg ggttagtccg
     2641 acataggcga ggcgacgctc ttcttcgagt ccggcacggc ccgaatcgtc cagcgagcgt
     2701 tggtgcggga aaagcccctc ctcccagccg ggcaaaaaga cgctttcgaa ttcaagcccc
     2761 ttcgccgcat gcaaggtcat gatcgaaacg cgggcatcgg actgcgtgtt cgccgcgtcc
     2821 atgacaagcg aaacatgttc gaggaaggca ccaagatcag gaaactcttc catggagcgg
     2881 acaagctctt tgagattctc gagtcttccc gttgcatcgg cggtgcgctc cttgcgccac
     2941 atgccgatat agcccgactc ctccaaaatg atctcggcaa gttccccttg cggctttgcc
     3001 tcgatcaatt gcgaccagcg ggaaagatcg ccgataagct cgcgcagcgc ctggcgttgt
     3061 ttcggcttta attcctcggt ctcgacgatg gccgcggccg cctgcgccag aggaacgcgc
     3121 acgcgtcgcg catgttcgtg gaggagctgc agggttgcct cgccaaggcc gcgtttgggc
     3181 agattgaaaa ttcgctcgaa ggcaagatcg tcggctggct gcgccacgca gcgcaaaaag
     3241 ccaagcgcat cgcgaatttc cgcacgctca taaaatcttg ggccgccgat cacccgatag
     3301 ggcaggccaa tggcaaggaa ccgttcctcg aactcgcgca tttgaaacga ggcgcgcacc
     3361 agtatggcaa tctcatcgag cacatggccc ttgcgctgca attcttcgat ttcttcccca
     3421 gtcacgcggg cctcttcttc tgaatcccag agcccggtaa cggctggttt ctcgcccaaa
     3481 gcgccatcgg taaaaagcgt ctttccgagc cttccctcat tatgcgcgat aaggccggac
     3541 gcggcggcga ggatatggcc ggtcgagcgg taattgcgct caagccttat aattttcgcg
     3601 cccggaaaat cgctctcgaa gcgcaaaata ttatcgactt cggcgccgcg ccagccatag
     3661 atcgactgat cgtcatcgcc gacgcaacaa aggttttgcc gcccctgtgc taaaagccgc
     3721 agccacaaat attgcgccgt attggtgtct tggtattcgt cgacaagaat atagcgaaat
     3781 cgctcgtgaa agagtttaag aatgtcagga ttgtcgcgaa aaagacgtat ggattcgagc
     3841 agaaggtcgc cgaaatcggc agcattgagc gtctttaatc tctcctgata aagcctataa
     3901 agcgcgctac ccttgccatt ggcgaaagcc gccgcctcgc ctgcgggtac atgcgcggga
     3961 tcaagcccgc gattcttcca agcatcgatt tgattggcga gcgagcgcgc cggccagcgc
     4021 ttctcatcga tattctcggc ctgcagcacc tgcttcagca aacggatctg atcgtcggta
     4081 tcaaggatgg tgaagctcga tttaagacca acaagctccg catggcggcg caagattttt
     4141 gtcgaaatcg cgtgaaaagt cccgagccaa gccatcgcct ccgcaaccgg ccccgcgagc
     4201 gtcccaagcc gcgcctgcat ttcccgcgca gctttgttgg tgaaggtcac ggcgagaatc
     4261 tcctgcgccc gcgcccggcc ttcggccagg atatgggcga tccttgtggt cagaacgcgg
     4321 gtttttccgg tgcccgcgcc agcaagcaca aggacagggc catcgattgt ctcgatcgct
     4381 gcgagctgtt cgcggttgag cccgtcgaga taacgcaacg ccacagcccg cgcgcggagg
     4441 ctgatcggcg cggacgcatc ggttgtgcca agtgcaaaaa cgttggtcaa gaaattgcgg
     4501 ctccgtttcg atccaagagc gactataccg aaggccctcg cgaatcagcc cattatatct
     4561 cccacagtcc aaattggcgt gatgatgatt ttgcgcaatg ctcgaaatgg cgacatttcc
     4621 aacagacgtg gctgctgtgc cctcaattct tagcaatcct atcccccagc tgacacaaaa
     4681 accaagttgg tgtgtcctac aaggcacagt cagacgcttt tatgggtaca cctgttgagg
     4741 ccgcacgaca acaataggcg gccaagatat aaactaccaa aactatacgg cacgggggta
     4801 tcatggatac gaaaccaatc tgccgcatgc ccttgatcct tgcactggca cttatatcgc
     4861 tttttatatt gggaggatgt gccgtagtag atcagtatag ttcgaaagca atagaatata
     4921 acgagcagac ggccgcgtca aaaaactcga gtgtattgct taatattcta cgggccgcgt
     4981 accgcgagcc cctgcaattc actgaggtca ccacggtcac aggaacggct gcggctcagg
     5041 gcggtttaag cgcggacatt ccactcagag tgg
//
LOCUS       DATMPU010000068         6968 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_4391467,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000068 DATMPU010000000
VERSION     DATMPU010000068.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 6968)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6968)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6968
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4391467"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(450..638)
                     /locus_tag="VNY06_01580"
     CDS             complement(450..638)
                     /locus_tag="VNY06_01580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="neprosin family prolyl endopeptidase"
                     /protein_id="HWX13564.1"
                     /translation="MADVAGNDWVGYYPGSIYGGGQLSGYVQEIDFGGETVRSRIWPP
                     MGPTAASRSRTALFIAPK"
     gene            complement(917..1924)
                     /locus_tag="VNY06_01585"
     CDS             complement(917..1924)
                     /locus_tag="VNY06_01585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012454168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX13565.1"
                     /translation="MPRYLLEPSGRTKQGQAPHWPLAAQLVAAALCLMPSFAKAQVAT
                     VDNPSVPALRLPLTPEAAPGERARGMVHLSAQFAANDPQPVHQGLQWRIFDEDLGPDG
                     NHKLVAQSSDATPILPLPDGNFVVHAALGLAGATKRITINGTVLNETLVLNAGGLRIT
                     GQLGDTPVSPAKLSVSIYVPEPVNPEAKLVVANAKSGDTIGLPEGNYHIVSTLLDAGS
                     NGSPSPTNSVISADLRVQAGKLTDATLRHRAATMTLKLENSPGAEALANTAFTVLTPG
                     GDVIREMIGAFPSATLAEGEYVAIARHDGKTYQATFKVMSAVDRDVEVLTREAPTPEA
                     P"
     gene            complement(1929..4457)
                     /locus_tag="VNY06_01590"
     CDS             complement(1929..4457)
                     /locus_tag="VNY06_01590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012335937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX13566.1"
                     /translation="MRPESISAMPQLDQSEVPQSGLPGQDWTPHAFRLALLGLLTFYG
                     LALASWLIGATVVPWDSKNHFYAMFRFVGDAMHNGELPFWNPYHFGGHPAIADPQSLI
                     FTPSMMLFALLAPKASMQLFDAVVLAHLLAGGICMLGLLRRWRWHPLGAVLAAIIFML
                     GGAASSRLQHTGIIISYSFFPAALWCLDIMLERCSYRFVIAFGVAASVMALGRDQVAF
                     LLCTVLISYVAYAAAQSGSTLAYLRARAGVLLLSGALILAIMSVPVLLTMQFLGDSNR
                     PGFPFGVAAAGSLAPVNLITLVAPNFFGSLDHLYDYWGPEYNTMPRADWTDRAVDYLF
                     IGTLPFLLIAWHGFGAGRLFARRAQIFVFLLLLAAAYALGRYTPFFALAFDWVPGVSL
                     YRRPADATFLINIALALAAGFLLHRYIEDGLPRPFSVLPKWQARAFAVATIAVLAILT
                     GAALDFSWRQGHLKNSLLALTMASVVAGTGAALLFGLQARRARVLAASLFVFFSAGDI
                     LWRNAASTLNAEPAARYGVYAGIDSAEAKGIELLRHEIAGRLRAGDRPRVEILGLKGP
                     WQNASIVLKLEDTLGYNPLRIDDYERAVGTGQDAEELAFRRYPDTFRGYNCQLAALLG
                     LQYLVLDRPLTELPRDAPRPKAEALYTSASMYIYKLGKTVPRAYFASAVKRVDNEDIL
                     EEQFIPGFDPSKDVLIDKASMVDLHGGPYAKDVFKPRIRTASLSDVKGVRQKPWEAAA
                     ASEGKALGEQDQPYATIVHYADNAVDIDIAAPKAGIAVLHDLYYPGWEAYVDGVQKPV
                     LRANILFRGVEVPAGRHTVSFVFRPLSMTNLVAALSSVMHRTEE"
     gene            complement(4529..4735)
                     /locus_tag="VNY06_01595"
     CDS             complement(4529..4735)
                     /locus_tag="VNY06_01595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX13567.1"
                     /translation="MGTSEFASFAAKTSICDAIFFVGYTSSFILAIQALGELNGIELR
                     TAFLRLRLRNEFTAKAAFFAFDLP"
     gene            5469..6698
                     /locus_tag="VNY06_01600"
     CDS             5469..6698
                     /locus_tag="VNY06_01600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003612327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HWX13568.1"
                     /translation="MRKFNSLSEYATKRFRLAGKAYLESGDPFFAPIDILRRDAERSG
                     RRFVSFANYDYLGLASHPAVKAAALATLQKLEIGALASRLVGGERSLHRTLESGLAKF
                     LGVESALALVSGYLTNVTTISHILGSHDALFIDELSHNSIVSGAKATVAETVVFRHND
                     LDHLDFLLREKRASYRNALIVAEGLYSMDGDIADLVRLVELKERRKAWLLIDEAHSIG
                     VLGNNGRGLWEHSGVDPEQIDLIIGTLSKTLGSCGGFVAGNAEVIGWLRYTLPGFVYS
                     VGLSPVIAAAAQTALQLMQEESWRIERLRQNAELFMNLAHEAGLDTGPAIGRGVVPIL
                     FSGSYETLAASSHLMDHGYYVPPIIQIGVPKDQPRLRFFISALHTEAEIGGVIELLAM
                     REVERVVSPVRSLTAAT"
BASE COUNT         1575 a         1906 c         2046 g         1441 t
ORIGIN      
        1 ctcgctgcaa attgatcgtt cccggaaagc ggccgatcaa cgcattcaat ttctttgtga
       61 gacgtggcca tttattcaat ccatatctta acaatcttga tggaaagtta cgcttatgct
      121 gacaaacata tggggatcta caagcgctcc ctaaggctca acaataaccc gcgcttcatt
      181 tcgcatctaa tttttgcatt ggaagccgaa aatcaatggc ccggaatttg tgttaaaatc
      241 ttggttaccg ctgaaaaagg ctgcttgcag gaataaccac aatatttcaa ggccataggc
      301 tgtgcatgca aaaacgagta ggagacagcg gcgtctagcc cgaccatcca aggtgccgct
      361 cgttttttgg ggttttccaa ctgaaacctt gaaagtcgat ggattaccgt attactcccc
      421 aagcggcggc aaaagagttc gcaagccgct cactttggcg ctatgaagag cgcagttcgc
      481 gaacggcttg cggcagtcgg acccatcggc ggccaaatcc tcgagcgaac tgtctcgccg
      541 ccgaagtcga tttcctgcac ataaccgctg agctgtccac ctccatagat cgagcccgga
      601 tagtagccga cccagtcatt gccagccaca tccgccaatt gccgcggtag aggtaaaatg
      661 gccgaagccg cctcgccatc atccgaaaaa gaacctccat ccgtcgaaaa agaaaaccgc
      721 tcaggacggg tccgagcttt ccgcatgatc cgtcatcggt cacatgggat agccctgcta
      781 tcgtcgccga tgccactttc atcgtggtga tggccttgta tccgctctag tctagcggca
      841 taccccatcg cttttctcct gagcgatcac cggcccttcg cgctgatcct tgcggcaatt
      901 tttgccagcg gcggagtcag ggcgcctcgg gcgtgggcgc ttccctggtg agcacctcga
      961 catctctgtc gacggcggac atcaccttga aagtggcttg ataggtcttg ccgtcgtggc
     1021 gcgcaattgc gacatactcg ccctcagcga gcgtggctga aggaaacgcg ccgatcatct
     1081 cgcggatgac gtcgccacct ggcgtcaaga cggtgaaagc cgtattggcg agggcttcgg
     1141 ctccggggct gttctcaagt tttaaggtca tggtggcggc gcgatgacgc agcgtcgcat
     1201 cggtgagctt cccggcctga accctaagat cggcgctgat aaccgaattc gtcgggcttg
     1261 gcgaaccatt gctccccgca tcgagcaagg tagagacgat atgataattg ccttccggca
     1321 gtccgatcgt gtctcctgac ttggcattcg cgacgacgag cttcgcctcc gggttgacgg
     1381 gctcgggtac atagattgaa accgagagtt tggctggact gaccggcgta tctccgagct
     1441 ggccggtgat ccgcagcccg ccggcgttca gcacgagagt ttcgttgagc acagtgccgt
     1501 tgatggtgat gcgttttgtc gcgcctgcaa gccctaaggc ggcgtgaaca acgaaattgc
     1561 cgtcgggaag cggcaggatc ggggttgcgt cagaggattg cgcaacgagc ttgtggtttc
     1621 cgtcgggccc caggtcctcg tcaaaaatcc gccattggag gccttggtgc accggctgcg
     1681 ggtcattggc cgcaaattgg gccgaaagat gcaccattcc gcgcgcccgc tcgccaggag
     1741 cagcctcagg ggtgagcggc aggcgcagag cgggaacgga aggattgtcg actgtcgcga
     1801 cctgggcctt ggcaaacgat ggcattaagc acagcgcagc cgcaacgagt tgcgcagcca
     1861 aaggccaatg cggcgcctgt ccttgcttgg tgcggccgga cggttcaagc aagtaccgcg
     1921 gcaaagccct attcctccgt ccggtgcatg acgctcgaga gcgccgccac gagattggtc
     1981 atcgacagcg gtcgaaaaac aaagctgacc gtgtgacggc cggccggaac ctcgacgccc
     2041 cgaaacaaaa tatttgcgcg aaggactggt ttttgaaccc catccacgta ggcctcccag
     2101 cccggatagt aaaggtcatg gagcacggca attcccgctt tcggcgccgc aatatcgatg
     2161 tcgaccgcat tatctgcgta atgaacaatc gtggcataag gctggtcttg ctctcccaaa
     2221 gccttgcctt cggacgccgc ggctgcttcc catggctttt gcctaacgcc cttcacatcg
     2281 cttaggctcg cggtccggat acgcggtttg aagacatctt tcgcataagg gccaccgtga
     2341 agatcaacca tgcttgcttt gtcgatcagg acatccttcg acggatcaaa accgggaata
     2401 aactgctctt cgaggatatc ctcattgtcg actcgtttga ctgccgaggc aaaataggcc
     2461 ctcggcacgg ttttgccgag tttatagata tacatggaag cgctggtata gagcgcttcc
     2521 gcttttggtc tcggcgcgtc gcgcggcagc tctgtcaagg ggcggtcaag gaccaaatat
     2581 tgaagcccaa gcaatgccgc gagctggcaa ttatagccgc gaaaggtgtc tggatagcgg
     2641 cggaaggcga gctcctcggc gtcttggcct gtgccgacgg cgcgctcgta atcgtcgata
     2701 cgcaagggat tgtaaccaag cgtatcctcg agcttcagga cgatcgatgc attttgccac
     2761 ggtcccttga gtcccaatat ctcgactcgc ggccgatcgc cggcgcgcag ccggcccgcg
     2821 atttcgtggc gcaaaagctc gatgcctttg gcttcagccg agtcgatgcc ggcataaact
     2881 ccgtagcgcg cagcaggctc cgcattgaga gtcgaggcgg catttcgcca aaggatatcg
     2941 cccgcgctga aaaacacaaa caggcttgca gcgagcaccc gcgcgcggcg tgcctgcagg
     3001 ccaaacagca aggcggcgcc agttcctgcc acgacggacg ccatcgtgag cgcaagaagc
     3061 gaatttttta ggtgaccttg ccgccaggaa aagtcgagag ctgcgcccgt cagaatggct
     3121 aacaccgcaa ttgttgccac ggcgaacgcc cgtgcctgcc atttcggcag cacggaaaaa
     3181 gggcgcggca gcccatcctc gatatagcgg tggagcaaaa atccggccgc gagagcaagc
     3241 gcgatattta tgagaaaggt cgcatccgct gggcgcctgt aaagagacac gccgggaacc
     3301 cagtcaaagg cgagtgcaaa gaaaggagta tagcgcccaa gcgcgtaagc ggccgccaac
     3361 agcaacagaa agacgaagat ttgggcgcgc cgtgcgaaca gccgtcctgc tccaaaaccg
     3421 tgccaggcaa tgagcaagaa gggcagcgtg ccgataaaaa gatagtcgac ggcgcggtcg
     3481 gtccagtcag cacgcggcat ggtgttgtac tcaggccccc aatagtcata gaggtgatca
     3541 agggaaccaa agaaatttgg tgcaaccagt gtgatcagat taaccggcgc aagagagccg
     3601 gccgcggcga ccccaaaggg aaagcccggc ctgttcgagt cgccgagaaa ctgcatcgtc
     3661 aaaagaaccg gcacggacat tatcgcgagg atgagagcgc cgcttaacag caacacgccg
     3721 gcgcgcgcgc gcagataggc aagcgtgctg ccagattgtg cggcggcata ggcaacgtag
     3781 cttataagga ctgtgcaaag cagaaaggcg acttgatcgc gcccgagcgc catcacggac
     3841 gcggcgacgc cgaacgcgat tacgaagcgg taggagcagc gctccagcat aatatcaagg
     3901 caccataagg cggctggaaa gaaggaatag gaaatgatga tgccggtgtg ctggagcctc
     3961 gaggatgccg caccgccaag catgaagatg atcgcggcaa ggaccgcacc taaaggatgc
     4021 cagcgccaac gccgcaagag gccaagcata cagattcctc cggcgagaag gtgtgccagg
     4081 acgacggcat caaaaagctg catcgaagcc tttggcgcca agagcgcgaa cagcatcatg
     4141 ctcggcgtga agatcaagga ttgcggatcg gcgatcgccg gatggccgcc gaaatgataa
     4201 ggattccaaa acggaagctc gccattgtgc atagcgtcgc caacaaagcg gaacatcgcg
     4261 taaaagtggt ttttcgaatc ccatggcacg acagttgcac cgatgagcca agaggcgagc
     4321 gcgagtccgt aaaaagtaag gaggcccaac aacgccagcc ggaaagcatg cggcgtccag
     4381 tcctgacccg ggaggccgga ttggggcact tcagattggt cgagctgcgg catggcggaa
     4441 atggattcgg gtctcatgcg atggtgtaaa taattggatg ggttcggacc gatgctgggc
     4501 gtcacggtag gcagcctgcc gtaggagtct acggcaggtc gaaggcgaaa aacgcggctt
     4561 ttgccgtaaa ttcatttcgt aagcgcagac gcagaaaagc ggtccggagt tcgatgccat
     4621 taagttcgcc gagtgcttga atagccaaaa taaagctcga agtgtaaccg acgaagaaaa
     4681 tcgcatcgca aatagacgtt ttggcggcaa aagaagcaaa ttctgacgta cccaagactt
     4741 gcgccgagac gctgcgtggg ctgagccata acgcgtgtcg ttgaccatag atttgagatg
     4801 tctttccaga taaattgagc ggcgatatcg actaagggag ggaacgatgc cgcaacccgt
     4861 ccttaagtgg caatttgtct gcaactcggc aagacacaag taaacaataa gccatgaacg
     4921 cgccatgaac gcggcggctg ggcctaccta agaggcggac ttttgtgaga cgggcagcat
     4981 cccggcaagg atcgcgtcat ttcgccctgt gttggacatt ggcaagcttt gcagcggaac
     5041 ggaaacagac tcttgttcgt ctgaggaaaa aagcgcgtct tgacgggctg gcggtgtgtt
     5101 gcctggatcg ccactgattc tcgacgcgta aattggtcgc gcacaatgaa cttatggctc
     5161 ggttccggtg ccgcgaactt ggctttgtta cgttttgtaa ccggaattta cactagaaag
     5221 taagttaatg ataagaaata tgtttttgga ttatcaacag gaaggcggca agtgtacgta
     5281 aggtttttgg gaagttccgc ttttgctact atcggcgcat gatgctatgc cacgattcgc
     5341 ctgtgctggg tcttggcaat ccggaagggc ttagaagaaa aatgctagaa gaagaacgca
     5401 agacgtgact ttcgctgccc cggagtcttt gtcgactcgt gaattcacct ggacctccga
     5461 acgttaaagt gcgcaaattc aatagccttt ccgaatatgc cacgaaacga ttccgcctcg
     5521 ctggaaaagc ctatcttgag agcggcgatc cattctttgc gcccattgac attttgcgcc
     5581 gggatgccga gaggagtggc cggcggttcg taagcttcgc caattacgat tatttggggc
     5641 ttgcctcgca tcctgcggtt aaggccgcgg ctttggcgac gttacagaaa ttggagatcg
     5701 gcgcgctcgc ttcgcgcctt gtcggcggcg agcggtcctt gcaccgcact ttggaatctg
     5761 gacttgcaaa gttcctcggc gtggagtcgg cgcttgctct cgtcagcggc tatctgacga
     5821 atgtgacgac gatctctcat atccttgggt cccatgacgc cctcttcatt gatgagttgt
     5881 cgcacaacag catcgtgagc ggggcaaagg cgaccgttgc ggaaaccgtt gttttccgtc
     5941 acaacgacct cgaccatctc gattttcttt tgcgcgagaa gcgggccagt taccgcaacg
     6001 ccctcattgt cgccgagggt ctctatagca tggatggcga catcgcagac ctcgtccgcc
     6061 tcgtcgaatt gaaggagcgg cgcaaggctt ggctgctgat cgacgaggcg cattcgatcg
     6121 gtgtccttgg caataatggg cgcggacttt gggaacattc cggtgttgat ccggagcaaa
     6181 tcgacctgat tatcggcact ttgtcgaaga ctttgggttc gtgcggaggt tttgtcgctg
     6241 gcaatgcgga ggtgattggt tggctgcgct acacgctgcc cggttttgtc tatagtgtcg
     6301 gcctctcccc cgttattgcg gccgcagccc agactgccct ccaattgatg caggaagaga
     6361 gctggcgcat tgagcggttg cgccagaacg ccgaactctt catgaatctt gcccatgagg
     6421 ctgggcttga tacgggaccg gcaattgggc gtggcgtggt cccgatcctt ttctccggta
     6481 gttatgagac tttggcagct tccagccatc tcatggacca cggctattat gtgccgccga
     6541 ttattcaaat aggcgttccg aaggaccagc cgcgcctgcg tttcttcatt tcggcgttgc
     6601 ataccgaggc agagatcggg ggcgtcatag aattgctggc aatgcgggag gttgagcgcg
     6661 tggtctcgcc ggttcgttcg ctgaccgctg caacctgaag gctttgcccg ggttcaatcc
     6721 gcgtgacatg tcctgcgccg caagccaatc gcgtgatggt gctgtcgcag ccagttaaat
     6781 gccgccccat caagcagcgt tcatgagcac caacgcacgc gagatcgata tttttccggc
     6841 cgacgggcta cggaattttc tcgccttctg tcgcctgccg cccgcctgat ttggtaacct
     6901 cgtctttgct gaaagtcgct atggttcctc cggacgcgat gcaaatgatc aactcgagtc
     6961 ctcgcgaa
//
LOCUS       DATMPU010000069         3443 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_55328, whole
            genome shotgun sequence.
ACCESSION   DATMPU010000069 DATMPU010000000
VERSION     DATMPU010000069.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 3443)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3443)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3443
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_55328"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..470
                     /locus_tag="VNY06_01605"
     CDS             <1..470
                     /locus_tag="VNY06_01605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012592797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="topoisomerase C-terminal repeat-containing
                     protein"
                     /protein_id="HWX13569.1"
                     /translation="GGELVIKAGRYGPYVKYGKINASLPQGADPTTFPLEEAVALVAA
                     KEVGPGQKAVMQGRLLGEHPSGGAITVRAGRFGPYVNRGKTNAALQKGISPETVTLEE
                     AVRLIDDKEGTGSRKETKKTADAGPRKAAKKAPEPQNLAVDAPVKKPKKAKRA"
     gene            575..2845
                     /gene="rnr"
                     /locus_tag="VNY06_01610"
     CDS             575..2845
                     /gene="rnr"
                     /locus_tag="VNY06_01610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012386321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008997 - ribonuclease R activity
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HWX13570.1"
                     /translation="MAFITSEREVLGEKGRAKIGKREIARAFNLKGEARIALKTLLKE
                     LEAEGGVARRGKSLHRPGLLSKVVLANIAWRDPDGDLIAEPVEWDRAQGPVPRILVMS
                     KASGRPQALAPGLGDRALLRVEAVAGAGPDEPAYSGRVIKILPRARSPFLGIFRADAH
                     GGGQVVPVEKKNLDLGEFLVAPGDQGAAQDGDLVAIEALRAGRPSLPAAKVRERLGAI
                     DSEKSISLIAVHAHQIPHIFGLDALAEAERARPASRRGREDWRAIPFVTIDPEDAKDH
                     DDAVHAEPDLSKENPGGFAISVAIADVAAYVRPGSALDLEAQDRGNSVYFPGRVVPML
                     PERISNDLCSLRPEEDRPALAARITIAADGRKLSHSFHRVMMRSAAKLSYMQVQTAID
                     NGPRDDTAAGLREHVLVPLYGAYSALKIAREKRGPLDLDLPERKIILDEMGAVKSVII
                     PPRLNSHRLIEEYMILANVAAAEMLEARNQAFVYRVHDEPSLEKLNSLAEFLASLGIK
                     LAKGSVLRAAQFNGILARVGGTDNEHIVNEVVLRTQAQAEYAVENYGHFGLNLRRYAH
                     FTSPIRRYADLIVHRALISALDLGADGLEALTGKELAEIAARISAAERRAMAAERETS
                     DRLIAAHLAGQAGAEFKGRISGATSAGLFVRLDETGADGFIPAATLGDDYFRHDPARH
                     ALIGSRTGQIHQLGDIVTVRLVEVAPLAGALRFELLTESGPVQARRRPANAKGRARKK
                     SHTTSQLLSARKAAKR"
     gene            3185..3361
                     /locus_tag="VNY06_01615"
     CDS             3185..3361
                     /locus_tag="VNY06_01615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX13571.1"
                     /translation="MRRYGCTLRYGRPWSSILSLWILPIMKGGLVAHQWALRMHGFET
                     AQRPGSAIGKSDSK"
BASE COUNT          707 a         1014 c         1068 g          654 t
ORIGIN      
        1 agggcggcga gctcgtcatc aaggccgggc gctatgggcc ctatgtcaaa tatggcaaga
       61 tcaacgcctc cttgccgcaa ggcgccgacc ccaccacttt tcccctcgag gaggctgtgg
      121 cacttgtcgc ggccaaggag gtggggcccg ggcagaaagc ggtcatgcag gggcgcttac
      181 tcggcgagca cccttcgggt ggcgcgatca ccgtgcgcgc ggggaggttt ggcccttatg
      241 tcaatcgcgg caagacaaat gcagccttgc aaaaaggcat ctcgccggag acggtcacgc
      301 tggaagaggc ggttcgcttg atcgatgaca aggaagggac gggaagccgc aaagagacta
      361 agaaaaccgc cgatgccggc ccgagaaagg cagcgaagaa agctcccgag ccgcaaaacc
      421 ttgcagtcga tgcaccggta aaaaaaccta aaaaggcgaa gcgcgcttga aaagatccgc
      481 cggtgataaa aaatccggcc gatacgcatt taaacccgta acgccccctc cctctgggca
      541 gcgcagcgaa aacttgcctt cgcgcgcaga tattttggct tttattacta gcgagcgcga
      601 ggttttgggc gaaaagggaa gggccaagat cggcaaacgc gaaatcgccc gcgcctttaa
      661 ccttaagggc gaagccagga tcgctctcaa aactttgttg aaagagctcg aggccgaggg
      721 cggcgtcgca aggcggggca agagcctgca taggccgggg cttttgtcaa aggtcgttct
      781 tgccaatatc gcctggcgcg accctgacgg cgacctcatt gccgaacccg tcgaatggga
      841 ccgggcgcag gggcccgtgc cgcgtatcct tgtgatgtca aaggcaagtg ggaggccgca
      901 ggctctggct ccagggcttg gcgatcgcgc cctgttgcgg gtggaagcgg tggctggcgc
      961 cggaccggat gagcctgcct actccgggcg ggtcatcaaa atcctgccgc gcgcaagatc
     1021 gccgttcctt gggatttttc gtgccgatgc ccatggaggc ggccaggtcg tgccggtgga
     1081 aaagaaaaac ctcgatctcg gcgagtttct tgtggcgcca ggcgatcaag gcgcggccca
     1141 ggacggcgat ctcgtagcca ttgaggcatt gcgtgcggga aggccaagcc ttcccgcggc
     1201 aaaagtgcgc gaaaggcttg gcgccatcga cagcgaaaaa tcgatcagcc tcattgcggt
     1261 ccacgcgcat cagatcccgc atatctttgg cctggacgct ctcgccgagg ccgaacgggc
     1321 gagacccgcg tcaaggcgcg ggcgcgagga ttggcgcgcg atcccctttg tgaccataga
     1381 tccggaggat gccaaggacc acgacgacgc tgtccatgcc gaaccggact tgagtaaaga
     1441 gaacccgggc ggttttgcta tcagtgtcgc cattgccgat gtcgcggcct atgtgaggcc
     1501 tggctcggcg ctcgatcttg aggctcagga tcgcggcaat tcggtttatt ttccaggccg
     1561 ggtcgtgccg atgctgccgg agcggatctc caacgacctc tgttcgctgc gcccggagga
     1621 agaccgcccg gcgcttgccg ctcgcatcac aatcgccgcc gatgggcgca aattgagcca
     1681 tagctttcat cgagtcatga tgcgctcggc tgcaaagcta tcttatatgc aggtccaaac
     1741 ggcaatcgac aatgggccgc gggatgatac ggcggctggc cttcgcgagc acgttttggt
     1801 cccgctttac ggggcttata gcgccctcaa aatcgcgcgg gaaaagcgcg gcccgcttga
     1861 cctcgatctt ccggaacgca aaataatcct cgatgagatg ggcgctgtga aatccgtcat
     1921 catcccaccg aggcttaact cgcatcgcct catcgaggaa tatatgatcc tcgccaatgt
     1981 ggcggcggcc gaaatgttgg aagcgcgcaa tcaagctttc gtctaccgcg tgcatgatga
     2041 gccctcgctc gaaaaactca acagcctcgc cgaatttctg gcttccctcg gtataaagct
     2101 cgccaaggga tcggtgctgc gagcggcgca attcaacgga atattggcgc gcgtgggggg
     2161 cacggacaat gagcatatcg tcaatgaagt cgtgctgcgc actcaggcac aagcggaata
     2221 tgcggtggag aattacggcc attttggcct caacctgcgg cgctatgcgc atttcacttc
     2281 gccaatccgc cgctacgccg atctcattgt ccaccgcgcc ttgatttccg cgctcgatct
     2341 tggggcagat ggcctggagg ctcttacggg caaggagctt gcggagatcg cggcgagaat
     2401 ttccgccgcc gaacgaaggg ccatggcggc cgaacgcgaa acatccgatc ggctgatcgc
     2461 ggcccacctc gcgggccagg ccggggcgga gtttaagggg cgcatatcag gcgcgaccag
     2521 cgctggcctt ttcgtcaggc tcgacgaaac cggcgcggat ggtttcattc cggcggcaac
     2581 gcttggtgat gactattttc gccatgaccc ggcccgccac gcgctgattg gctcgcggac
     2641 cggtcaaatc caccagcttg gcgatattgt gaccgtgagg ctcgtggaag ttgccccgct
     2701 tgcaggcgcg ctccgcttcg agctcttgac cgaatccggc ccagtccagg cacgccgaag
     2761 gcctgcgaat gccaagggta gggcgcgaaa gaaatcgcat accacttcgc aactgctttc
     2821 tgctaggaaa gccgccaaac gatgagtctg ggacgcgaag cctgtcgccc ttgagagggg
     2881 cgggtattgg agagaatgat gctgcgcctg ttatggattc attaagattc gcccgcgagg
     2941 ttccttcttc ggccgccggc aagccgcgcg atgtgcggcc cgcgatccgc aaggggcttg
     3001 ccggccgttg ccccgcttgc ggtaaaggca agattttctg agcctatctc aaggtcagtg
     3061 acgaatgccc gacatgtggc gaggaattgc atcattaacg tgcagacgat gccccaccct
     3121 atatgacaat cgtcgttgtg ggtcacatcg tcggcacctt gttgcttgtg accgaggaag
     3181 ccggatgcgc cgctatggct gcacactgcg ctatggccga ccctggtcgt ccatcctatc
     3241 tctttggatt ttgccgatca tgaagggcgg tctcgttgcc catcaatggg ccttgcgcat
     3301 gcatggtttt gaaaccgcgc aacggcccgg aagcgcaata ggcaaaagcg acagcaagtg
     3361 agccgagtga caagcgcgcc aagtcttatt gcatccctca cgccgcgcga aactcttctc
     3421 tcgctcgcct cggccatcgt ctc
//
LOCUS       DATMPU010000070         4618 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_1604502,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000070 DATMPU010000000
VERSION     DATMPU010000070.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 4618)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4618)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4618
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1604502"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..504
                     /locus_tag="VNY06_01620"
     CDS             <1..504
                     /locus_tag="VNY06_01620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020176112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HWX13572.1"
                     /translation="PGVLFLNGSINAQLSVPIYQGGAEYAGIRQAKEALGQARLQADL
                     QRDLVRSSVVSTWGQLESARAVILSSKAAVTSSEIALDGVRQEAEVGQRTTFDILLQQ
                     QILLNARVNLVIAQRDRVVASYAVMEAIGRLTAANLNLTVTQYDPTIHFDQVKDKWIG
                     LRTPDGR"
     gene            776..1171
                     /locus_tag="VNY06_01625"
     CDS             776..1171
                     /locus_tag="VNY06_01625"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012591632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HWX13573.1"
                     /translation="MADVRVICAIGKRGQLGLNGQLPWEGNPGAEYKADVERFFALTR
                     GHVLIAGPRTIASFPGWAWSQMTLVEIHSTDKPQEVLADYSDRVVYIGGGPPVWKAYA
                     QFVNHWDVTRLPYDGDADRYFDPSWLCAW"
     gene            complement(1210..2412)
                     /locus_tag="VNY06_01630"
     CDS             complement(1210..2412)
                     /locus_tag="VNY06_01630"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018259972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HWX13574.1"
                     /translation="MSFLADAILRIKPSATIAVTQKARDLKNAGREVISLSTGEPDFD
                     TPDNIKKAAIAAIERGETKYTPVPGIPPLREAVSLKFRRENGLSYKPSQTIVGTGGKH
                     ILFNALLATVDRGDDVIIPAPYWVSYPDMVLIAGGTPVAVETKMEHGFKLQPEALEAA
                     ITPRTKWLILNSPSNPSGAAYTWTELKQLTDVLLRYPHVWILTDDIYEHLTYGDFKFA
                     TPAQVEPQLIDRTLTMNGVSKAYAMTGWRIGYAAGPEHLINAMDTLQGQQTSGACSIA
                     QWASVEALTGPQDFIAERRKIFETRRDLVVSMLNQAKSLNCPAPEGAFYVYPSCAETI
                     GKKTPDGRVISNDEDFATALLEAEGVAVVHGTAFGLGPNFRISYATATEALAEACAKI
                     QRFCASLA"
     gene            2693..2857
                     /locus_tag="VNY06_01635"
     CDS             2693..2857
                     /locus_tag="VNY06_01635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX13575.1"
                     /translation="MSIADNFVQKPAAGFVRAYDARAARRQFQISITLVCVLAFAAIA
                     LGVLTQLARP"
     gene            3118..3306
                     /locus_tag="VNY06_01640"
     CDS             3118..3306
                     /locus_tag="VNY06_01640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012383301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX13576.1"
                     /translation="MHFRLTPPGILIFFVSLILAAAAVATVYMHVPIVGHYVATHRFW
                     VMTAAYAVLLAGVIFERL"
     gene            3480..3989
                     /locus_tag="VNY06_01645"
     CDS             3480..3989
                     /locus_tag="VNY06_01645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013946914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HWX13577.1"
                     /translation="MSDLVVIAFPSEAKAEEVRQKVLELQKEYLIELGDAAIAVKQPD
                     GTIKLNQLVSTTAAGAVSGTFWGTLIGLIFLMPLAGAAIGAASGALGGYLTDIGVNDT
                     FMKEVAASLQPGNAALFLLVRKLTTDKVLEDLKGVGGTVLRTSFDHTKEDALRAALAG
                     VTQAAQPPA"
     gene            complement(4077..4496)
                     /locus_tag="VNY06_01650"
     CDS             complement(4077..4496)
                     /locus_tag="VNY06_01650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012591127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HWX13578.1"
                     /translation="MRQALHSLHIQTKGKGLVEIGGGVSLWIQQQNISTGLLTIFCRH
                     TSASLLIQENADPDVRTDLENFFEAIAPEDNKRYVHRTEGLDDMPAHIRAALSQTQLA
                     VPIVSRRMVLGTWQGIYLFEHRRAPHRREVVLHLFGD"
BASE COUNT         1010 a         1239 c         1340 g         1029 t
ORIGIN      
        1 ccgggggttc tgttcttaaa cggttcaatc aatgctcagc tttccgtgcc aatctaccaa
       61 ggcggcgcgg agtatgcagg aatccggcag gccaaggaag cgctcggtca ggcgaggctc
      121 caggccgacc ttcagcgtga tctcgtccgc tcgtccgtgg tctcgacttg ggggcaactt
      181 gaatccgccc gcgccgtcat cctgtcatcc aaggccgcag tcacgtcgtc cgagatcgcg
      241 ctcgacggcg taaggcaaga ggcggaagtc ggccagcgca cgacatttga tattctactg
      301 cagcagcaaa ttttgcttaa cgctagggtc aacttggtta ttgcgcaacg cgaccgcgtt
      361 gttgcatcct acgcggtcat ggaggccatt ggccgactga ccgcagcaaa cctgaacctc
      421 accgttacac aatatgatcc aacgatccat tttgaccagg tcaaagacaa atggatcgga
      481 ctgcgcacgc cggatggacg gtaaggcaat cgtggccgtc accgtttggc agtcaatcct
      541 tccatgccat tggttaaagt ccatgccgac tgcactggga ggcagagtat aggaatttag
      601 ctgccaccgg ccgtggatcg ctttggagca atacgcctcc aaatgacgcg aacgccatga
      661 gcatagccca ggagatttgg aataacggag cgctgccgtc ccattgcggc agaatgatct
      721 aacaaatcgc tggggcgtgc acgcggaaaa aaaccaatgc cgcttggaga gaacgatggc
      781 cgatgttcgc gtcatttgcg ccataggaaa acgaggtcag ctcggtctca atgggcagct
      841 tccctgggag ggcaatccgg gagccgaata caaggccgat gtggagcgat tttttgcttt
      901 gacccgaggt catgtgctga tcgccgggcc gcggacgatc gcctcttttc caggctgggc
      961 ctggtcacaa atgacgttgg tggaaattca ttccacagac aaaccgcaag aggtcttggc
     1021 ggactattct gatcgcgttg tctatattgg aggcgggccg ccagtctgga aggcctatgc
     1081 gcaattcgtt aaccattggg acgtgacgcg cctgccttac gatggtgacg ccgaccggta
     1141 tttcgatcct agctggttat gtgcgtggta accgtgaatc attgggttgt gggatgaccg
     1201 gccttgtact caggcgaggc ttgcacagaa acgctgaatt tttgcgcagg cttcggcaag
     1261 cgcttcggtg gccgtcgcat aggaaatcct aaaattgggg ccaagcccaa aggccgtgcc
     1321 atgcacgacg gcaacccctt ccgcttcaag aagcgctgtg gcaaaatcct catcattgct
     1381 aatcactctg ccatcgggcg ttttcttgcc tattgtctcg gcgcaagagg gataaacata
     1441 gaaagcccct tcaggggccg ggcagttcaa agattttgcc tggttgagca tcgagaccac
     1501 aaggtctcgc cgcgtctcaa aaattttgcg ccgctcggca atgaaatcct gcgggccggt
     1561 tagcgcctcg accgatgccc attgcgcgat cgagcaggcc cccgaggtct gctggccctg
     1621 caaagtgtcc atcgcgttga tcaagtgctc cgggcctgcg gcatagccaa tccgccagcc
     1681 ggtcatcgca taagcctttg agacgccatt catggtaagc gtgcggtcaa tgagctgcgg
     1741 ctcgacctgt gcgggcgttg cgaatttaaa gtcgccatag gtaagatgtt cgtaaatgtc
     1801 gtcggtcagg atccaaacat ggggatagcg cagcaagaca tcggtgagct gctttaattc
     1861 cgtccaggta tacgccgcgc cggaggggtt tgacggtgag ttcaaaatta gccatttggt
     1921 cctcggcgta attgcggcct cgagcgcctc gggttgcagc ttgaagccgt gctccatttt
     1981 tgtctcaacg gcaaccggtg tgccgcccgc aatcagaacc atatcgggat agcttaccca
     2041 atagggggca ggaatgatca cgtcgtcacc gcgatcgacg gtggcgagaa gggcattgaa
     2101 caatatatgt ttgcccccag tgccgacaat tgtctgcgac ggcttgtagc ttaatccatt
     2161 ttcgcgcctg aacttgaggc taactgcctc gcgcaaaggc ggtatgccgg gaaccggcgt
     2221 atatttcgtt tcgccacgtt cgattgcggc aatcgccgcc ttcttgatat tgtctggcgt
     2281 atcgaagtca ggctcgccag ttgaaagtga aatgacttcc cgtccggcat ttttgagatc
     2341 gcgcgccttc tgcgtaaccg cgatcgtcgc agatggtttg atgcgcaaaa tggcatcggc
     2401 aagaaaagac atttggacta tctccgcgcg gaaaaacagg gcgcgcttac gggcatgcgc
     2461 gagaaaggac ttggaccata ggtatgtagt taccagaagg caaccggaaa caccgctggc
     2521 gaaatcggcc ttaggcgggc agatttacgg cgaattaaca ttgaacctac ttccgcagtg
     2581 catcggccca agaaactaaa tatcaagcag gaactttaaa cggggcatca gagtgttggc
     2641 gaggaaatgg ggtttggcgg gaatgccttg ccgccgaaga ggagattgcg gaatgtcgat
     2701 cgcggacaat tttgtacaaa agcccgccgc cggatttgtc cgcgcctatg atgcgcgggc
     2761 tgcaaggcgg caattccaaa tttcaatcac gctcgtttgc gtgcttgcat tcgcagccat
     2821 cgcgctcggc gttctgacac agcttgccag gccatagccg cggggcgttc tgcgaaaaag
     2881 gatgggcagc acagatgtcg cggaaatttt tttagtcacc cgcgcttcat taggcaggtt
     2941 tctcgcccgc ttttcgcctg gatcctcgag aagccgccaa aagcaattgg cgacgcgggc
     3001 agctcagtcc aggttcagaa ctatttgcgg cagcttgcca aatggccaaa ttttcgccgc
     3061 gcggcttcaa tctacggtaa gttaagcggt tgaccttcat ccgagccgga ttatgctatg
     3121 cacttccgat tgaccccgcc gggcattctc atattcttcg tctctcttat ccttgccgcc
     3181 gcagcggtcg cgaccgtcta tatgcatgtt ccaattgtcg ggcattatgt cgccacgcac
     3241 cggttttggg tgatgacggc ggcttatgcg gtgttgctag cgggcgtgat cttcgagaga
     3301 ttgtagcaat gtcctcggaa atggcctacg cttggcaaga ggcgcctcgg gttcaagcag
     3361 cgtgttccgc ttcacattcg tgacgtattc aataggatat agaccgggtc cgtgtagtgg
     3421 ccctgcgcgg gaacaaaaaa aaacaaattg ctgcgaatga cacaggagag aaacacgcca
     3481 tgagcgatct tgtcgttatt gcatttcctt ccgaagccaa ggccgaggag gttcgccaaa
     3541 aggtgctcga actgcaaaag gagtatctca ttgaacttgg cgatgccgcg attgccgtaa
     3601 aacagcccga tggtacaatc aagctcaatc agctcgtgag cacgacggcg gccggggcag
     3661 tctccggtac tttttgggga acgctgatcg gtttgatttt cctgatgccg ctcgctggtg
     3721 ccgcgatcgg cgccgcctcc ggcgcactcg gcggctatct gactgacatt ggggtcaatg
     3781 acacttttat gaaagaggtc gcggctagct tgcagccggg caatgcggca ctctttttgc
     3841 tcgttcgcaa gctgaccact gacaaagttc tcgaggatct gaagggcgtt ggcggcacgg
     3901 tcttacgcac ttccttcgat cacacgaagg aggacgcgct gcgcgccgct ctagctggag
     3961 taacccaggc tgcgcagccc ccagcgtgaa tgaggacccc ggctactctc gctcttaagc
     4021 gcgtcgccag tgggtccctg gctgaatggg gccagagccg ttgcgcagcg gcgcgtctag
     4081 tctccgaaga gatgcaagac gacttctctg cggtgcggcg cacgcctgtg ttcaaaaaga
     4141 taaatcccct gccaggtccc gagcaccata cgccgggaga caattggaac agcaagctgc
     4201 gtttgcgata gcgcggcccg aatatgcgca ggcatgtcgt ccaagccttc cgttcgatgg
     4261 acatagcgtt tgttgtcttc tggggcaatt gcttcgaaaa agttttcgag atccgttcga
     4321 acatcagggt cggcgttttc ctgaatgagg agcgaagccg aggtgtggcg gcagaaaatc
     4381 gtcaaaagcc cggtcgagat attttgctgc tgaatccaaa gcgagactcc gccgccgatc
     4441 tcaacaaggc ctttgccttt ggtttgaatg tgcagggaat ggagcgcttg gcgcattgga
     4501 atgatcgagg attcgagcgg gactatgccg cgtgtttaaa aactcaggat ttggtacgcg
     4561 ggtcaatgtc cgatatcacc tttcatcgtc ggccaccatc aaaggcgaga aactcgcc
//
LOCUS       DATMPU010000071         5818 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_4082816,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000071 DATMPU010000000
VERSION     DATMPU010000071.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 5818)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5818)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5818
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4082816"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(81..157)
                     /locus_tag="VNY06_01655"
     tRNA            complement(81..157)
                     /locus_tag="VNY06_01655"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(121..123),aa:Arg,seq:ccg)
     gene            complement(392..580)
                     /locus_tag="VNY06_01660"
     CDS             complement(392..580)
                     /locus_tag="VNY06_01660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020174562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HWX13579.1"
                     /translation="MSRETHLAALEQRHDALDKEIERESVHPARDELKLTELKRRKLQ
                     LKDEIAKLQCDRPLLSTL"
     gene            956..1174
                     /locus_tag="VNY06_01665"
     CDS             956..1174
                     /locus_tag="VNY06_01665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012590954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF465 domain-containing protein"
                     /protein_id="HWX13580.1"
                     /translation="MTEKLSEEDRALLQAEFERLRQEHRDLDAAIEALLQIGSGDRLQ
                     IQRLKKRKLALRDRLAFLEDQLTPDIIA"
     gene            2421..3476
                     /locus_tag="VNY06_01670"
     CDS             2421..3476
                     /locus_tag="VNY06_01670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012386104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWX13581.1"
                     /translation="MEHQILDRDRLIVRRLIDALPGAVVAIDAADRIVAVNAPSKDFF
                     PALRLDDLLARSLRTPDVLDAVKRARASGVPERVTWLERVPVERFFEANVAPMNGPFE
                     PTMILTLRDLSEVRRVERMRVDFVANASHELRTPLASLLGFVETLQGPAREDKPAREK
                     FLAIMRDQAQRMARLVDDLLSLSRIEQNLHLRPQAPVDMVGILRHMADTLAPMAAEND
                     VRLLLEVPAKAVVTGDRDELIRVAENLIENAIKYGASDPRCVNRQVKITLAAEARQCV
                     MTVCDYGPGIAPEHLPRLTERFYRVDAGQSRAKGGTGLGLAIVKHIVARHRGRLGIDS
                     KLGQGATFSVTIPLHES"
     gene            3755..4723
                     /gene="pstS"
                     /locus_tag="VNY06_01675"
     CDS             3755..4723
                     /gene="pstS"
                     /locus_tag="VNY06_01675"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013946236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HWX13582.1"
                     /translation="MPPGAGATFPYPLYAKWADTYKKDTGVGLNYQSIGSGGGIKQIK
                     ANTVTFGASDMPLKPAELKEAGLVQFPTVVGGVVPVINIEGIKPGELVLDGPTLAKIF
                     LGEITSWDDPAIKKLNPKVKLPSLAIVVVHRSDGSGTSFLFTDYLSNESPDWKSKVGS
                     STAVEWPAGIGAKGNEGVANNVTQTKGAIGYVEYAYAKQNKMPYAGMINKDGKRVVPN
                     TESFSAAAAGADWANAPGFYLILTDQHGAKSWPIAGATFILMHTVPDDPATAAEALKF
                     FSWAYQNGSKMAEELDYVPLPSPVVALIKKKWAAEIKGADGKPLLN"
     gene            4867..>5818
                     /gene="pstC"
                     /locus_tag="VNY06_01680"
     CDS             4867..>5818
                     /gene="pstC"
                     /locus_tag="VNY06_01680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011501312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HWX13583.1"
                     /translation="MANNVAVDNQGLNAAEARLGKALAGFKFRDTTFHLLTLAAAWVV
                     LLLLGGVIVSLVIGAMPSIRAFGLNFYTTESWNPVTEKFGAMAPMYGTIVTSIIAMTI
                     AVPSAIGIAIFLTELCPYWLRKPIGIAIELLAGIPSIIYGIWGLFVFAPFLQQYVQPP
                     LIALFADVPVLSVLFAGPPYGIGVLTAGIILAIMVLPIVTSISRDVFDTVPPVLKEAA
                     YGVGCTTWEVISSVVIPYTRIGLLGGVMLGLGRALGETMAVTFVIGNAHRIAPSLLAP
                     GTTISATIANEFTEAVRDLYTSSLIQLGLILFVITFIILAI"
BASE COUNT         1265 a         1635 c         1567 g         1351 t
ORIGIN      
        1 gcgggcaatc ttagcgctta ctatgtgctt attttcaaat ttctagcact aaaagaaaat
       61 atctaagcta ttgattttat tggcgcaccc gaagggattc gaacccctgg cctctgcctt
      121 cggagggcag cgctctatcc agctgagcta cgggtgcctt gaagcagcgt gatcttaaca
      181 gaagtctact gacgcgcgct atctgggata accccgccgc gttttagcag aacttcttgg
      241 agactgaagg cttgccacga aatggccgtt gcattccctc taaccgaggg gcaccggcta
      301 agtccaacgc ccgcaacgcc cctgcgatgg gcgcggtcgc tgcggccaga ccagccggca
      361 ccgccgcttt acagtttaaa agcgatgaaa ttcagagtgt actaagcaat gggcggtcgc
      421 actgcagctt ggcaatttca tccttgagtt gcagcttccg ccgtttcaat tcggtcaatt
      481 ttaactcgtc cctggcggga tgcaccgatt ccctttctat ctccttgtcg agcgcatcat
      541 gacgctgttc gagagcggcc aaatgagttt cgcgtgacat gcagaactcc ttcttccgaa
      601 ccacataaga ttcgcaagtg tgccacagga ttctccattc gcgaagccgc ttcgttggtc
      661 atttcgatca aattttcggc ctttggggac atcgttaata aataattcag gaatcggcta
      721 cctttgcctg gccgaagtaa aaaaacagct ggagatggtt acgaggcatg atgggttgca
      781 tagaccagtg gcgacaaacc cgcggataaa caatagctta gccttgaggg taaacggcgc
      841 gagcattgcg aggcacagcc aagagagctg tacgccaccc tgttgtttag tccgttcatc
      901 gattgagcta gtatagcggc ccagaaaatt ggatggccca gagaaatggc tgcgcatgac
      961 agaaaaacta agcgaggagg accgcgctct actgcaagcg gagtttgagc gcttgcgaca
     1021 ggagcaccgt gatctcgatg ccgcgatcga ggcgttgctc caaattggca gcggcgaccg
     1081 gctgcaaatc cagaggctta agaagagaaa acttgcgctg cgcgatcggc tggcatttct
     1141 tgaggatcag cttacgcccg atatcatcgc ctgatcgtgg tgcgccaacg gagctcgttt
     1201 gccgctcgct cgcagagcga aggcgatttg tcataagtga cctgcaggta gcaactaaca
     1261 agcatttcga agaatctggt accagaacag gtttcggcga agtatttagt aacctgctat
     1321 tgttttagct ttgatcttgt aatataaata ttcctatgta tcgagttgtg ctaacgatta
     1381 ttgaatagag gtaggtttct gacgctggcc ttctgtgtct gtccggatag aagttgaatc
     1441 tgctgaatct gttgtgattc aagggaggcg gcctgatttg aggaggggcc gccgatggcg
     1501 accaaccccg ctgcctctgt cacacaaagg gaatggaact caggattaag aagccctgtt
     1561 gcaaccctga actcagaaga cattttttcc gcttggccac ggccctgaca tcgaagaagc
     1621 gaggagtaaa ccgattaaaa aaattcttct catccttcgg tacagcctca acggtaattt
     1681 tatttacagt gacgtcggaa ctccgattga agaacttccc tgcgtggaaa ccatcattta
     1741 accccgcgta aagaaccaga ttttctacct aaggctgaga aagagaaagg cttttggacg
     1801 aggcgctggc gatgaataaa tgacggaacc aaactggaaa tgtgtttact gctgctcccg
     1861 cgccgagggc tattccaata cttataacag caactcctct tcgcatcttc cagtacctcc
     1921 aataagttta ttttcctgac tcccaccggc gccgagttgg tgcgactgca gatagggttt
     1981 cacggcacgg gcatcgcggg acccatgttc cctgtttccg acataagcaa gatacgcagt
     2041 agcgttcgca gcggcgttct cgagcattcc ggcggcggca tcacgccgtg caagcaagat
     2101 caacggaccc tattggggtt tggcgtagag caagatgact tttcttcgaa tcttcatctt
     2161 gctctaattc cattgtcgca tgtccggacg ccaaatcggt ttccactttt ggctcgatcc
     2221 gctctaagat accttcttcg ttgcaagacc gcctagacag tcggttagga cgaggacctc
     2281 aagcctagtc aggcccaacc aactttgtct gtgccgcaaa ttgctgcttt cgccgtgtca
     2341 tcagcccgac atcaatctga gacttgatat tagcgcgccc tcctgtccat aaggcctgcc
     2401 gatcgggctt tgggggctcc atggaacacc aaattctcga ccgtgaccga ttgattgtgc
     2461 gcagactcat cgatgccctg ccgggggcgg tcgtggcaat cgacgcggcc gaccgcatcg
     2521 tcgcggtcaa cgcgccgtca aaggatttct ttccagcatt gcgactggac gatctcctcg
     2581 cacgaagcct ccgcacgcca gatgtactcg atgcagtcaa acgagcacgc gcttcgggcg
     2641 ttccggaacg cgtgacttgg ctcgagcgcg ttccggtcga aagatttttt gaagccaatg
     2701 tcgctcctat gaatggaccg ttcgaaccaa cgatgatctt gacattgcgc gatttgagcg
     2761 aggtgcgccg cgtcgaacgc atgcgggtcg attttgtcgc caatgcgagc cacgaactgc
     2821 ggacgcctct tgcctcgctt cttggctttg tcgagaccct tcagggaccg gcacgcgagg
     2881 acaaaccagc aagagaaaaa ttcctcgcca ttatgcggga ccaggcccag agaatggcgc
     2941 gcctcgtcga tgaccttcta tccttgtcgc ggattgagca gaatttgcat cttcggccgc
     3001 aggcgcccgt ggatatggtc gggatcttgc gccacatggc cgacaccttg gcccccatgg
     3061 ctgccgaaaa tgatgttcgc ttgttgcttg aagtacccgc caaggccgtc gtgacgggag
     3121 atcgcgacga gttgatccgg gtcgccgaga atcttatcga gaatgccata aaatacggtg
     3181 cctcagatcc ccgctgtgtc aacaggcagg tcaaaatcac gctcgcggcc gaagcgcgcc
     3241 aatgtgtcat gacggtttgc gactatggtc ccggcattgc accggaacat ctgccgcgat
     3301 taactgaacg tttttatcgg gttgacgccg gccagagccg cgccaagggc ggcaccgggc
     3361 ttggccttgc tatcgtcaaa catattgtcg cgcgccatcg cggccggctc ggcatcgact
     3421 cgaaacttgg ccaaggggcg acctttagcg tcacgatccc cttgcacgaa agctgaggcg
     3481 aaaacgcccg gcgtcatcag actgtcaaat aagtttcata ctggcctcac cggttcccta
     3541 tagcgtggga accgggcgag ggcttggctt agctatgtcc ggcttccggc gcccggccgg
     3601 acttatctgc cggcccgcat tccgaccctg tttcgagcgg caccatcacc ttgctgccga
     3661 tgtaaaatag ccaaagcgag gcatgaaatg acgttttctt gcatgcgaac ggccttcgcc
     3721 gccgccttaa gccttatgac aagcctccca gtcgttgccg cctggcgccg gcgcgacctt
     3781 cccctatccg ctctatgcaa aatgggccga tacttacaaa aaggacaccg gcgtcggcct
     3841 taactatcaa tcgatcgggt ctggcggcgg catcaagcag atcaaggcca acaccgtgac
     3901 tttcggtgcg tccgatatgc cgttgaagcc cgccgagctg aaggaggccg gcctcgttca
     3961 gtttccaacg gtggtaggcg gcgttgtgcc ggtgatcaat atcgaaggga taaagcccgg
     4021 cgagctggtc cttgacggcc cgacgctcgc caagatattt cttggggaga ttacaagctg
     4081 ggacgacccg gccatcaaga agctgaaccc aaaagtcaaa ttgccatcgc tggcgattgt
     4141 cgtcgttcac cggtccgacg gatcgggaac gagctttctc ttcaccgatt atctttcgaa
     4201 cgaaagccca gactggaaat ctaaggtcgg ctcgagcacc gccgtcgaat ggccggctgg
     4261 tatcggcgcc aagggcaatg aaggcgtcgc caacaacgtc acccagacga agggggcgat
     4321 cggctatgtc gaatatgctt atgccaaaca gaacaaaatg ccttatgccg gtatgatcaa
     4381 caaggacggc aaacgtgttg tgcccaacac cgagagcttt agtgcagcgg cagccggtgc
     4441 agactgggcc aatgcccccg gcttctatct aattctcact gaccagcatg gcgccaagtc
     4501 ctggccaatt gcgggcgcca cttttatcct gatgcacacg gttccagacg acccagcgac
     4561 tgctgctgag gcgttgaagt ttttcagctg ggcctatcag aacggcagca agatggccga
     4621 ggaacttgac tatgttcccc tccccagccc ggttgttgcc ttgatcaaga aaaaatgggc
     4681 ggccgaaatc aagggcgcgg acggcaaacc cttgctcaac tagagcgctg gccggagggc
     4741 cgctcaggcc actccggcca gttcataccg tcccttcgtg gttttcggta tcgcgggcag
     4801 caggttgcgt catccttagc ggctctttag gctcgaggat aatgcaaaat cgaaaggcaa
     4861 agtacgatgg ccaataatgt ggcggtcgac aaccaaggat taaacgcggc cgaggcccga
     4921 ttgggcaaag cgcttgcggg tttcaagttc cgtgacacga cgtttcacct tttgaccttg
     4981 gccgccgctt gggtcgttct cctgcttctc ggcggggtga tcgtctcctt ggtcatcggc
     5041 gctatgccgt ccattcgcgc tttcggactc aatttttata cgacggaatc gtggaatccg
     5101 gtcaccgaaa aattcggtgc catggcgccg atgtatggca cgatcgtcac ctccataatt
     5161 gccatgacga tcgcggttcc gtccgcgatt ggcatcgcaa tttttctgac tgagctttgc
     5221 ccctattggt tgcgcaagcc gatcggtatc gcaattgaac ttttggccgg gattccatcg
     5281 atcatttatg ggatttgggg gctttttgtt tttgctccct tcctgcaaca atatgtccag
     5341 ccgccactca ttgcgctttt tgccgatgtc cccgttcttt ccgtgctgtt tgccggcccg
     5401 ccctatggca tcggcgtgct gacggcagga atcattttgg cgatcatggt gctgccaatt
     5461 gtcacctcga tttcccggga cgtcttcgac acggttcctc cagtcctcaa ggaggcagcc
     5521 tatggcgtcg gatgcaccac ttgggaagtg atcagctcag tggtgatccc ctacacgcgc
     5581 atcggccttc tgggcggcgt catgctaggc ctcggccgtg cgctcggcga gaccatggct
     5641 gtgacctttg ttatcggcaa cgcccataga atagcacctt cgctgctcgc gccaggcacc
     5701 acaatttcgg cgacgatcgc caatgagttc accgaagctg tccgcgatct ttacacctcg
     5761 tcgcttattc agctcggcct gatcctcttc gtcatcacct ttatcattct cgcgatct
//
LOCUS       DATMPU010000072         2197 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_3471351,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000072 DATMPU010000000
VERSION     DATMPU010000072.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 2197)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2197)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2197
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3471351"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(422..1714)
                     /gene="gltA"
                     /locus_tag="VNY06_01685"
     CDS             complement(422..1714)
                     /gene="gltA"
                     /locus_tag="VNY06_01685"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012590699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004108 - citrate (Si)-synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HWX13584.1"
                     /translation="MSATTGFFNIGDKTVEMPVKTGTIGPSVVDITKLYAQTGLFTFD
                     PGFTSTASCESEITYIDGDEGVLLYRGYPIEQIAEHGDFLETCYLLLYGQLPTPAQKK
                     DFDYRITRHTMVHEQFARFFHGLRRDSHPMAVLVASVGALSAFYHDSTDISDPTQRMV
                     ASLRLIGKMPTLAAMAYKYSLGQPFIYPKNDLDYTSNFLRMCFAVPCEEYKLSPVFAR
                     ALDRIFILHADHEQNASTSTVRLAGSSGANPFACIAAGVACLWGPAHGGANEAALKML
                     MEIGTVENIPKYVARAKDKNDPFRLMGFGHRVYKNYDPRAKIMQRTCHEVLTELGIKD
                     SSLLEVAMALEKIALSDEYFIEKKLYPNIDFYSGITLKAMGFPVSMFTVLFAVARTAG
                     WIAQWKEMIEDPSQKIGRPRQLYKGPAMRDYVPIENRA"
BASE COUNT          515 a          587 c          613 g          482 t
ORIGIN      
        1 aagagctgcg acggcaagga aaacgtcagc aaccgcaatt ggggtgccat ttagtgaaaa
       61 aaggacgcgc tccatgccgc gatccttaac tgattcgctg tatcatggga acgaataatg
      121 aacatctgaa ttaggcgcaa aaacgtcgcg ccgcctcaat tgtgctcgtc cactgtaagc
      181 ctgtcagaac tgaaaattga cgaaactttc tcaaggacac caaagggcat gatgtcggag
      241 taaagcggcg cggctaaaaa gcgatcgcaa ggaacggata gcacgcagct ttgcagagat
      301 gtctggcctg gcaagattaa gacagctggc ttgcggtcaa gcttatgtca attttctgcg
      361 gatggagtcg aggcgcgtgc caggcctaac ctgcaaagac ttgcggtgtt agcggatgcc
      421 gttatgcgcg attttcgatc ggaacatagt cccgcatagc cgggccttta tataactggc
      481 gcgggcgtcc aattttctgg ctcggatcct cgatcatctc cttccattgc gcgatccagc
      541 ctgcggtgcg cgccaccgcg aaaagtacag taaacatgga caccggaaag cccatcgcct
      601 tcagtgtgat tccggaataa aaatcgatat tcggatagag ctttttctcg atgaagtatt
      661 catcgctgag ggcgatcttt tcgagcgcca tcgcaacctc aagcaacgaa ctatctttga
      721 ttccgagctc ggttaggacc tcgtgacatg tcctttgcat aattttcgcc cgcgggtcat
      781 agtttttgta gacccgatgg ccgaacccca tgagccgaaa tggatcgttc ttgtctttgg
      841 cgcgggcgac atactttgga atattctcga ccgtgccgat ctccatcagc attttgagcg
      901 cggcctcatt ggcgccacca tgagccggac cccagaggca agccacgcct gccgcgatgc
      961 aggcaaacgg atttgcgccg gacgaaccgg caagccgcac tgtcgaggtc gacgcatttt
     1021 gctcgtgatc agcatggagt atgaagatcc gatcaagcgc gcgggcaaaa actggactga
     1081 gcttatattc ctcgcatggc acggcaaagc acatgcgcaa gaaattcgac gtataatcga
     1141 ggtcgttctt cggatatatg aatggttggc caagcgaata tttgtaggcc atagcggcaa
     1201 gcgtcggcat tttgccgatc aggcgaagcg aagcgaccat tcgctgggtc ggatccgaaa
     1261 tgtcggtgga atcgtgataa aaggccgaca gtgcgccaac actcgccacc aacacggcca
     1321 tgggatgaga gtcacgccgc aggccatgaa agaacctcgc aaactgctca tgcaccatcg
     1381 tgtgccgcgt gattctataa tcgaaatcct tcttttgcgc aggggtaggc aattggccgt
     1441 aaagcaacag gtagcacgtc tctaggaaat cgccatgctc ggcaatctgc tctattggat
     1501 agccgcgata aagcagcacg ccttcgtcac catcgatata ggtgatctcc gactcgcagc
     1561 tcgccgtcga agtgaacccc ggatcaaaag taaacaggcc ggtttgcgca taaagcttgg
     1621 tgatatccac gacggagggg ccgatcgtcc cggtcttgac gggcatttcg acggtcttgt
     1681 cgcctatatt gaagaagcca gtcgtcgcgc tcatggggcc tgctttctaa aggttcatag
     1741 cggtgagcct ggcaagtctc cggctccgcg ccacgataat gatgcagcgc aacagcacta
     1801 gctcatacag acaattcttt caagcgggca cactgccttt gcacggcggc aaaatgaggg
     1861 cttccggaac ccaatgctca attattgtcc ggtcgctaaa agtgctgctg aaggagccga
     1921 acgccgctga ccatgttgtc ggctctggcg acaaatcttc tcgtggagtt tggttggcgc
     1981 cgctttggca gcggccacac tgtggtgaga cggcaccgcg ggggttgccg cctgacagcg
     2041 tagtttgacg gctttatcct cctaacgcgg ccgccgcaac taaagaggcg cgtggccgtc
     2101 acattgtggc ggccacgacc ttgttgtctt ggagaatttg gcggggagcg gtcttcgccg
     2161 caaacggcgg ctctaacgtt aggccggacc ttcttgc
//
LOCUS       DATMPU010000073         3385 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_4395123,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000073 DATMPU010000000
VERSION     DATMPU010000073.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 3385)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3385)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3385
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4395123"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..363
                     /locus_tag="VNY06_01690"
     CDS             <1..363
                     /locus_tag="VNY06_01690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020087303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF983 domain-containing protein"
                     /protein_id="HWX13585.1"
                     /translation="ALEASSSAGRQPRNVWPAIRKGIAGHCPACGIGRIFRAYLKVND
                     TCPTCGEELYHQRADDAPPYMTIVVVGHIVVGLLVVTEEYWPDAPLWLHVAIWPALAL
                     TLSLWLLPIMKGALIAYQ"
     gene            441..1622
                     /locus_tag="VNY06_01695"
     CDS             441..1622
                     /locus_tag="VNY06_01695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020175809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWX13586.1"
                     /translation="MTSAPSLIASLTPRETLLSLASAIVSVTIVGIGLSLTMPLLALR
                     LDQQGFSARAIGLNSTAGGLAVLVGAGAVPALVRQFGVKRLLLLSILISCISLLNFAL
                     TNNYWTWLAIRAVFGGALSTLFVTSEFWINAVAPPRQRGFVLGFYATSLSAGFAAGPM
                     ILGFTGTEGISPFLAGVVLFALASAPIVIWSGKAPVIKSVPNAGLLAILTCVPLATIA
                     ALLHGAIETASLGLLPVYALRAGDSPQTGALLVSLFALGNVVFQLPIGFVSDHMARGK
                     LLVFLASISLCGAVAMATAGPERFLLFCFLLVIWGGVVGSLYAVGLAHLGSRYSGADL
                     ASANAAFIMLYALGMIIGPPIAGFGMDLISPNGLFFSIAAMLGLYLILAWLRNARIST
                     G"
     gene            1746..2495
                     /gene="ubiE"
                     /locus_tag="VNY06_01700"
     CDS             1746..2495
                     /gene="ubiE"
                     /locus_tag="VNY06_01700"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012591243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HWX13587.1"
                     /translation="MVEETHFGFSHVPLKDKQGRVDEVFDGVAPRYDLMNDLMSGGLH
                     RVWKDIFISKIKPSKKKRFRHIDVAGGTGDIAFRVAKAGSSLTEVVVLDINAAMLQEG
                     RRRAAKQHFEAALDFVEANAEELPFKENSFDTYTIAFGIRNVPRIGKALNEAHRVLRR
                     GGRFLCLEFSDVNVPLLDRLYEAYSFHVIPLLGRYFAGDDKPYSYLVESIARFPDAES
                     FCGKIGGAGFARASFTRLSGGIVAIHSGWKA"
     gene            2612..>3385
                     /locus_tag="VNY06_01705"
     CDS             2612..>3385
                     /locus_tag="VNY06_01705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012591244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HWX13588.1"
                     /translation="MPSSFGSFLRLLRAGLVLARAGVFSDLDPAILPPAARLPLRLAH
                     LLGPRRAVTPKAKAAGGLANLPAAISRLGPSYVKLGQFLATRPDVVGTRVAQELELLQ
                     DRMAPFSRRVAIATIEAAFGKPLDLIFTSFEEPVAAASIAQVHKARVKGRVGNGEVAV
                     KVLRPGVERFFKRDLSDMYFAARFAERWSSASRRLKLIEVVDTLARTVTMEMDFRLEA
                     AAASEFAENTAQDKDFCVPKVDWDRTTKEVLTLEWVDGTP"
BASE COUNT          596 a         1002 c          970 g          817 t
ORIGIN      
        1 gcccttgagg cttcatcctc ggccggccgc cagccgcgca atgtgtggcc tgcgatccgc
       61 aagggaattg ccggccattg ccccgcctgt ggtatcggca ggattttcag agcctatctc
      121 aaggtcaatg acacctgccc gacatgtggc gaggaattgt atcatcaacg cgccgacgat
      181 gcgccaccct atatgacaat cgtcgttgtg ggtcacatcg tcgtcggctt gttggttgtg
      241 accgaggaat attggcccga tgcaccgctg tggctgcacg tcgccatatg gccggccctg
      301 gcgcttacct tatcgctttg gcttttgccg atcatgaagg gcgccctcat cgcctatcaa
      361 tgagccttgc gcatacatgg ttttgaagcc gcgcaacggt ccggaagcgc aataggcaaa
      421 agcgacagca agtgagccga gtgacaagcg cgccaagtct tattgcatcc ctcacgccgc
      481 gcgaaactct tctctcgctc gcctcggcca tcgtctcggt gacaattgtc ggcatcggtt
      541 tatcgctgac catgccgctg cttgcgctgc ggctcgatca acaaggattt tccgcgcgcg
      601 cgattggcct taattcgacc gctggcgggc ttgcggttct tgtcggcgcc ggcgccgttc
      661 ccgccctggt gcgtcagttt ggcgtgaagc ggctcttgtt gctctcaatt ctcatcagct
      721 gcatcagtct cctgaacttt gctctgacga ataattattg gacttggctg gccattcgcg
      781 cggtcttcgg cggcgcgctc agcacgctct tcgttaccag cgaattttgg atcaatgcgg
      841 tcgctccgcc gcgccagcgt ggcttcgtct taggatttta tgcaacgagc ctctcagccg
      901 gttttgctgc tggtcccatg attttaggtt tcaccggcac cgaaggcatt tcgcctttcc
      961 tggccggtgt ggtgctgttc gcactcgctt cggcaccgat tgtcatttgg agcggcaaag
     1021 cgcccgttat caaaagtgtc ccgaacgccg ggcttctcgc cattcttacc tgcgtgccgc
     1081 tggcaacaat cgccgcattg cttcatggcg caatcgaaac cgcaagcctt ggcctgttgc
     1141 cggtctatgc gctgcgcgct ggcgatagcc cgcaaaccgg agcgctgctc gtcagtctct
     1201 ttgcactcgg caacgttgtg tttcagctcc caattggctt tgtctccgat catatggcaa
     1261 gaggcaaact tttggtcttt cttgcctcca ttagtctttg cggagctgtt gccatggcta
     1321 ctgccgggcc cgagcgattt cttctctttt gtttcctgct tgtcatatgg gggggcgtcg
     1381 tcggcagcct ttacgcggtc ggcctcgcac accttggttc gcgctatagc ggcgcggatt
     1441 tggcgagcgc aaatgcagcc ttcatcatgc tctacgcgct gggcatgatc atcgggccgc
     1501 ccattgctgg cttcggcatg gatctcatct cgccaaatgg gttgttcttc tctatcgccg
     1561 ctatgctcgg actttatctc atccttgcgt ggttgcgcaa cgcgcgcata tcgactggat
     1621 gagtgttggc ggacggcgtg tcatgcccga aagcagacaa atggccatga cgctcgcccc
     1681 gcgctgtgct aacccggacc ggagctccat tgattactcc ccaacctctg tctgcggaca
     1741 agtaaatggt tgaagaaaca catttcggct tttcgcacgt tcccctaaaa gacaaacaag
     1801 gtcgggtgga cgaggttttt gatggggtcg cacctcgcta cgacctcatg aatgatctca
     1861 tgtccggcgg cctccaccgc gtctggaaag atatttttat cagcaagatc aagccatcca
     1921 agaaaaaacg cttccgccat attgatgtcg cgggcggcac cggcgacatc gcctttaggg
     1981 tcgcgaaggc aggctcgagt ttgaccgagg ttgtagtcct cgacattaac gctgccatgt
     2041 tgcaggaagg gcgcaggcgc gccgccaaac agcattttga agcggcgctg gactttgtcg
     2101 aggcaaacgc ggaagagctg ccgtttaaag agaacagttt cgatacctat acgatcgcct
     2161 ttggcattcg caatgtgccg cgcattggca aggcgctcaa tgaggcgcat agggtgctgc
     2221 gacgcggcgg cagatttctt tgtcttgaat tttcagacgt gaatgtgcct ctcctcgatc
     2281 gcctctacga ggcctattcg tttcacgtga tcccgcttct cggccgctat tttgcaggcg
     2341 atgacaagcc ttacagctac cttgtcgaat cgattgcgcg ttttcccgat gccgagagtt
     2401 tttgcggcaa gatcggcggg gcaggctttg cgcgggcctc atttacgcgg ctttcgggcg
     2461 ggatcgtcgc cattcattcc ggctggaagg cgtaggccat gttctctgat cgcacgcaag
     2521 tcggcaacca tctggtaatt cgcatgcatg accgcttgcc ctgctctccg gctttgccgg
     2581 atcgagcccg gccttagcgc gggcggcggt tttgccttct tccttcggat cctttttgcg
     2641 gttgctgcgc gcgggattgg tgcttgcgcg cgcgggcgtc ttcagcgacc tcgaccccgc
     2701 gatattgccg cccgccgcgc gcttgccttt gagactggcg cacttgcttg gcccgcgccg
     2761 ggcagtaacg ccaaaggcca aagccgctgg cggtcttgcc aaccttcccg ccgcgatcag
     2821 ccggctcggt ccatcctatg tcaagctcgg tcagttcctt gcgacgcggc ccgatgttgt
     2881 cggcactaga gtggctcaag agctcgaatt attgcaagat cggatggcgc cgttttcgcg
     2941 ccgagtcgcc attgcgacaa tcgaggccgc attcggcaaa ccgctcgatt tgatctttac
     3001 aagcttcgaa gagcctgttg cagcggcatc gatcgcgcaa gtgcacaagg cgcgggtgaa
     3061 aggaagggta ggcaacggcg aagtcgccgt caaagtgctg cgtcccggtg tcgagcggtt
     3121 ctttaagcgg gatctctccg atatgtattt cgcggcgcgt ttcgccgagc gctggtcgag
     3181 cgcgtcacgc cgcctcaaac tcatcgaggt cgtcgatacg ctcgctcgca ccgtcacgat
     3241 ggaaatggat ttccggcttg aggccgctgc agcatccgaa ttcgctgaaa acacagcaca
     3301 ggataaggat ttctgcgtgc caaaagtcga ctgggaccgg accacgaaag aggtgctgac
     3361 gctcgaatgg gtcgatggaa caccc
//
LOCUS       DATMPU010000074         3758 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_365416,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000074 DATMPU010000000
VERSION     DATMPU010000074.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 3758)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3758)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3758
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_365416"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..678)
                     /gene="hemH"
                     /locus_tag="VNY06_01710"
     CDS             complement(<1..678)
                     /gene="hemH"
                     /locus_tag="VNY06_01710"
                     /inference="COORDINATES: protein motif:HMM:TIGR00109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HWX13589.1"
                     /translation="MASSNVSATSNKPVPHGNIGILLVNLGTPDAPRFWPVRQFLKEF
                     LSDPRVIETPRIFWWPILHFIILVPRPRRIAKHYAAVWNKETDEGPLKAITWSQAEKL
                     ATWIGAGGLDPKRQHAARETFFIAWAMRYRKPDIAQGIVTVTEHGCTRILVLPLYPQY
                     AAATTASVHDEIFETLKAMRWQPAVRIAPPYFDDRTYIDVLATSLRAGLSRLDFVPDT
                     ILVSFHGL"
     gene            complement(881..1564)
                     /locus_tag="VNY06_01715"
     CDS             complement(881..1564)
                     /locus_tag="VNY06_01715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018267886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HWX13590.1"
                     /translation="MQQSKKPPATTLDRLTLVRARIARAARDAGREPQDVTLVCVSKT
                     FAAQDIAPVIGAGERVFGENRVQEALEKWPPLKADNPGLELHLIGPLQSNKAHDAVLL
                     FDVIETVDREKIAKALASEIQKTSRHPHLYVQVNTGAEPQKAGVLPQEADAFIANCRK
                     KFDLEISGLMCIPPLDDKASPHFAFLNQIAARNGILALSMGMSADFELAIELGATHVR
                     IGSAIFGER"
     gene            complement(1576..2061)
                     /locus_tag="VNY06_01720"
     CDS             complement(1576..2061)
                     /locus_tag="VNY06_01720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX13591.1"
                     /translation="MPRSIHAAFLAATVGAVTILAVGTARSQDAKSICARVTNDDRVR
                     RLPITLVPAARQLFGFSADTPSGYVRKGTSFRCMNGKVFLCNTGANLVCGKANTSRTS
                     AGAEDFCKQNPGSDVVPMAATGHDTVYEWKCVGNKAVISKHAETVDPRGFITENWKQL
                     D"
     gene            2353..2904
                     /locus_tag="VNY06_01725"
     CDS             2353..2904
                     /locus_tag="VNY06_01725"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012589179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HWX13592.1"
                     /translation="MKLSTLLLSLCVGLFATAAWAALKPGDAAPDFTVNAALGGKEFT
                     FSLTEALKKGPVVLYFYPKSFTSVCTAEAHEFAENIENFAAAGASVIGLSGDKIEVQR
                     DFSAKECRDKFPVGADPSFSVIKAYDAAFNIPVVGAAFANRISYVISPEGKILYAYSD
                     SHAEKHIENTLAVVKKWRAERRP"
     gene            complement(3176..>3758)
                     /locus_tag="VNY06_01730"
     CDS             complement(3176..>3758)
                     /locus_tag="VNY06_01730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX13593.1"
                     /translation="LAEHPPNDILERRESSAVAAVSPPDDLTASLSEAISSGRAQQAF
                     NELLKRIEDRNRALSAEAMLEKLKGADQMDALTRRELFAVVLEKQSQRILNLMRYVID
                     GAKAHHPLLGGLSPMLDRLIAFDLSTANGLSNEATAALGLARQALLTQMLDDYAGGRL
                     DSDRLAMLIGGGPGAAIVGRAIMEPIISSIPER"
BASE COUNT          767 a         1077 c         1124 g          790 t
ORIGIN      
        1 caggccgtgg aaggaaacca ggatcgtatc gggaacgaaa tcgagacgcg acagcccagc
       61 ccgaagggaa gtcgccagaa cgtcgatata ggtgcggtcg tcgaaatagg gcggcgcaat
      121 ccgcaccgcc ggttgccagc gcattgcttt caatgtttcg aaaatttcgt catggacgct
      181 cgccgtcgtg gcggcggcat attgcggata aagcggcagg accaggattc tcgtgcagcc
      241 atgctccgtg acggtcacga ttccttgcgc gatgtccggc ttgcgatagc gcatcgccca
      301 agcgatgaag aatgtttcgc gcgccgcgtg ctgccgttta ggatcaaggc cccctgcccc
      361 gatccaagtc gcgagctttt cggcctgcga ccaggttatc gccttcagcg gaccctcgtc
      421 cgtctccttg ttccaaaccg cggcatagtg cttggcgatc cggcgcgggc gcggaaccag
      481 aatgataaag tgcaggatcg gccaccagaa aatccgtgga gtctcgataa cccgcgggtc
      541 ggacaaaaac tccttgagaa actggcgcac cggccaaaag cggggggcat ccggcgtgcc
      601 aaggttgacg agcagaatgc cgatatttcc gtgaggaacc ggtttgttgc tcgtcgcgga
      661 gacattggag gacgccatat atctgagact cgcttatcga ggtggtccat tgcggcagca
      721 cctttagggc gcattcgttg gaaacggaaa tgctaaagcg acacgccgcg cgatgtttcc
      781 gcatcttcgt gccgagcgga attcgcacgg attcgatgac gcattcgtga acgttccacg
      841 gccttctcgg aataggcgcc ggaatcagag ggcggcactc tcagcgctct ccaaaaatcg
      901 cgctgccgat ccttacatgg gtcgcgccga gctctatggc gagctcgaaa tcggcgctca
      961 ttcccatcga gagcgccaaa atcccattgc gcgcggcgat ctggtttaaa aacgcgaaat
     1021 gcggcgaggc cttatcgtcg aggggcggaa tgcacatcaa acctgaaatt tcgaggtcga
     1081 attttttacg gcagttcgcg atgaaggcgt ccgcctcttg cggaagaaca cccgccttct
     1141 gcggttcggc tccggtattg acctggacat aaaggtgggg atggcgggag gttttttgaa
     1201 tttccgaggc gagcgccttg gcgatctttt cccggtccac ggtctcgatc acatcgaaaa
     1261 ggaggaccgc gtcatgcgct ttattcgact gcagggggcc gatgagatgg agttcgaggc
     1321 cgggattgtc cgccttgagc ggcggccatt tttctagcgc ctcctggacc cggttctcgc
     1381 caaaaacccg ctctccggcg ccgatcacgg gcgcaatgtc ttgcgcggca aaagtcttgg
     1441 agacgcagac gagggtcacg tcttgcggct cgcgtccggc gtcacgcgcc gcgcgggcga
     1501 tgcgggcacg caccaaggta aggcggtcga gggtggtggc gggaggtttt ttgctttgtt
     1561 gcaaagcttt tacctttaat ccagctgctt ccagttttcg gttatgaagc cgcgtggatc
     1621 gaccgtttcg gcatgtttgg agatgaccgc tttatttcct acgcatttcc actcgtagac
     1681 cgtgtcgtga cccgtggcgg ccatggggac gacatccgaa ccagggtttt gtttgcaaaa
     1741 atcctctgcg ccggccgacg tgcggctggt attggccttc ccgcaaacga gattcgctcc
     1801 cgtgttacac aaaaaaacct tgccgttcat gcaacggaag gaggtgcctt tccgaacgta
     1861 tccgctcggt gtgtcagccg aaaagccaaa aagctggcgt gccgctggga cgagagtgat
     1921 cggaaggcgt ctcacgcgat cgtcattggt gaccctcgcg caaattgatt ttgcgtcctg
     1981 cgagcgggcc gtgcccacgg ccaagatcgt cacggcgccg acggtcgcgg ccaagaaagc
     2041 cgcgtggatg gatcgaggca tgatggctcc gttcgtcaaa acgatttgga aagttacctc
     2101 ccgagattgg ccgcggtaac ccatcgatca acgcgaagcg ggtgcttttc ataacagccg
     2161 ggcgggccgt tccgcaaagc cgccggcaca caggcttgag atatgtggga ttgcgccgaa
     2221 aattggtagt gaacgcctga tgacaaccag cgaaaaaaac gcgtccggtc actccgcttt
     2281 gatttgatcg ggaggagcat tatgtgttgt tgaactgtca cttgacgagc acgaaactaa
     2341 aggatgcgcc caatgaagct atcgaccctg ctcctctctc tttgtgttgg cctcttcgca
     2401 accgccgcat gggcggcgtt gaagccggga gacgccgcgc ccgatttcac cgtgaacgcc
     2461 gcgctgggcg gcaaggagtt cacattctcc ctcactgagg ctttgaagaa gggaccggtg
     2521 gttctctact tttatcccaa atccttcacg agcgtctgca ccgccgaggc gcatgaattt
     2581 gccgaaaaca tcgagaattt cgcggcggct ggagcgagcg tcattggcct ttcgggcgac
     2641 aagatcgagg tgcagcgcga cttttcggcc aaggagtgcc gcgacaaatt cccggttggc
     2701 gccgacccaa gcttttcggt gataaaagcc tatgacgccg cgttcaacat tccggttgtg
     2761 ggcgcggcct ttgccaaccg catttcctat gttatttcgc ccgagggaaa gattctttac
     2821 gcctactccg attcccacgc ggaaaaacac atcgagaaca cgctggccgt cgtgaagaaa
     2881 tggcgcgcgg agcgcaggcc ctgaagctcc gggtctctcg cactaccgtt tgcctcgaac
     2941 cattgttggc tatctcgtca ccagtgagtc cgccgggcgc gcgaaaatgt cttatccgga
     3001 gcggaaatcg taccaaaatg tttcacgtga aacattttgg tacgatcggg gccgaaaagc
     3061 ttacggcgct catacgtcgc gcggccttgg gacatgtggg attgcacgga aaattggtat
     3121 tgttggcggt tggatggcgc ggccgcgcgc ggcgcgagcg acacgggcac ccacgctagc
     3181 gctccggaat cgacgaaatg atcggctcca ttatcgctcg accaacgatg gcggctccgg
     3241 ggcccccgcc aatcagcatc gccagacggt cactatccag gcgaccgcca gcgtagtcat
     3301 ccagcatctg cgtcaaaagc gcctgtcgcg ccaggccgag cgcggctgtc gcctcgtttg
     3361 ataggccatt tgccgttgac agatcgaacg caatgagccg atcaagcatc ggggacagcc
     3421 caccaaggag cgggtggtgc gccttcgctc cgtcgattac atatcgcatc aaattcagta
     3481 tccgctgcga ctgtttctca agaacgaccg cgaacaactc tctgcgcgtc agcgcgtcca
     3541 tctgatcggc acctttgagc ttctccagca tcgcctccgc gctaagagcc cggttgcgat
     3601 cttcaatccg cttcagtagc tcgttgaatg cttgctgtgc tcggccgctg cttatggctt
     3661 ctgagaggct agcggtcaga tcatctggtg gggacactgc ggccacggcc gaagactctc
     3721 gtcgctcaag aatgtcattt ggcgggtgct cggccaga
//
LOCUS       DATMPU010000075         3783 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_2535656,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000075 DATMPU010000000
VERSION     DATMPU010000075.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 3783)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3783)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3783
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2535656"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1227)
                     /gene="glcF"
                     /locus_tag="VNY06_01735"
     CDS             complement(<1..1227)
                     /gene="glcF"
                     /locus_tag="VNY06_01735"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019963207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcF"
                     /protein_id="HWX13594.1"
                     /translation="MQTNFSLAQLRNPATAEAEKILRACVHCGFCTATCPTFLLLGDE
                     LDSPRGRIYLIKDMLERNRPADETVVKHIDRCLSCLACMTSCPSGVNYMHLVDHARSH
                     IEATYKRPLADRLLRKFLGFVLPYPDRFRLALAAAPLARPLKTLYPRLGRTGRRLAAM
                     MRLAPVSGRKAPASPRYAAPPPTSAPKARVALFRGCVQQVLRPEINDAAIRLFSRLGV
                     ESVQLSGEGCCGALVHHLGKETAALAQARANIDAWSAEIERNGLDAIVITASGCGTMI
                     KDYGFLLRNDEAFAERAARVSTLTKDVTEFLASRELAAPVSPQHKVVAYHSACSMQHG
                     QKIDAVPRELLRQAGFEVCDIPEGHLCCGSAGTYNILEPEIAERLRDRKIKNIIAASP
                     DVIATGNIGCITQLATG"
     gene            complement(1229..2428)
                     /locus_tag="VNY06_01740"
     CDS             complement(1229..2428)
                     /locus_tag="VNY06_01740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008593891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HWX13595.1"
                     /translation="MSHFIPADQAEVAEVVTEAVAKHAPLSIIGLGTKSALGRPVRNP
                     CRLDLSRLDGISFYEPEELVLSALAGTPIATIEALLAASGQELAFEPMSFAALYGTGH
                     GTIGGSLMANLSGPRRIKAGAARDFVLGLKAVSGRGKAFKAGGRVVKNVTGYDLSRGL
                     AGSFGTIAVATEITLKVLPRAETSATLLLFGLDPGSAIKALCAAMGAPADVSGAAHLP
                     SFPAATQGFENAITALRVEGFAPSVEGRLALLETLLKNFAPMAKLDAPASEGLWHAIR
                     DVAPLAEAREKIVWRLSVAPTAGPKIAEAVGRAHQVEVLFDWSGGLVWLALDPCPDGA
                     VVLIREAIAQNGGGHASLIRGPVELRKKIPVFEPQPGALASLSRRLKEAFDPRAILEP
                     RRMWAEF"
     gene            2579..3532
                     /locus_tag="VNY06_01745"
     CDS             2579..3532
                     /locus_tag="VNY06_01745"
                     /inference="COORDINATES: protein motif:HMM:NF012703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginase family protein"
                     /protein_id="HWX13596.1"
                     /translation="MMKFAVITFPYTTDTDLGTAAGPEALLRAGLTNWLREKGHDLAG
                     PFHVKLSACEEAASGAWNKLGFANAHLARLVSDAVRNRAFPLILESNCYAAIGVWAGL
                     QMSAGKASQRLAMVWIDAHGDCNTPETTLSGMLSGMPVAIAIGLCLDRFRKQSGLYAP
                     IDQRDTVMVCVRANDPLEQELIDQLEIEMVPVLDIKGDCRKLRAAMEHLSRSVNAIYV
                     HFDVDALDETEVASMWLTAPDGPTARELAAAMEIVMAFPKIAAFGVSDINPERDVEGQ
                     MVHAALTVIKGGIAALPGKSTLRHSGSRGRAPASREKGKRQ"
BASE COUNT          811 a         1065 c         1149 g          758 t
ORIGIN      
        1 tccggtcgcg agctgggtaa tgcagccgat attgcctgtg gcgatgacgt cgggcgatgc
       61 cgcaataata tttttgatct tgcgatcgcg cagccgctca gcaatctccg gctccaatat
      121 attgtaggtg ccggctgagc cgcagcataa atgtccttcc ggaatatcgc agacttcgaa
      181 acctgcttga cgcaaaagct cgcgtggcac ggcatcaatt ttttgaccgt gctgcatcga
      241 acaggcggag tgataggcga ccaccttgtg ctgtggactg accggagcgg ctagttcgcg
      301 cgaggcaaga aattcggtca cgtcctttgt gagcgtggaa acccgcgcag ccctttccgc
      361 aaaggcctcg tcattccgca gcaggaaacc ataatccttg atcatcgtgc cgcagcccga
      421 cgccgtgatc acaatcgcgt cgagcccatt gcgctcgatt tccgcgctcc aggcatcgat
      481 gttggctcgc gcctgggcca gcgcagccgt ttccttgccg agatgatgga ccagcgcgcc
      541 gcaacagcct tcgcctgaca actgaacgga ctcgacacct agtcgagaga ataagcggat
      601 cgcggcatcg ttgatttccg gccgaagaac ctgctgaacg cagccgcgaa aaagcgcgac
      661 gcgggctttt ggtgccgatg tgggcggcgg tgcggcatag cgcggagatg ccggggcttt
      721 tcggccagaa actggtgcaa gtcgcatcat ggcggcaagc cgccgccctg tccggcccaa
      781 gcgtggatag agagttttga ggggtcttgc caaaggcgcc gcggcaagtg cgaggcggaa
      841 gcggtctgga tacggcagaa cgaagccgag aaatttccgc aacaaccggt ccgccagcgg
      901 ccttttgtac gtcgcctcaa tatggcttct cgcgtggtcg acaagatgca tataatttac
      961 gcccgacggg caggaggtca tgcaggcgag gcaggaaagg cagcggtcga tatgtttgac
     1021 caccgtctca tcggctggcc tattcctttc gagcatgtcc ttgatgagat agattcgccc
     1081 gcgcgggcta tcgagctcgt cgccgagcaa taaaaaggtc gggcaggtcg cggtgcaaaa
     1141 gccacaatgc acgcaggcgc gaaggatctt ttccgcttcc gctgtcgccg gatttctgag
     1201 ctgcgcgagc gaaaaattcg tctgcatttt aaaattccgc ccacatgcgc cggggctcca
     1261 gaatggcgcg aggatcgaaa gcctccttca gccgccgcga cagagaggca agcgcaccag
     1321 gctgcggctc gaagaccggg atttttttgc gcaattcaac gggcccgcgg ataaggctcg
     1381 cgtggccgcc gccattttgc gcaatagcct cacgaatgag caccaccgcg ccatcggggc
     1441 aagggtcgag ggcaagccaa accaggccgc ccgaccaatc aaacaggacc tcgacttgat
     1501 gcgcgcggcc caccgcctcg gcgattttcg gtccagcggt cggagcgacg gaaagtctcc
     1561 agacgatttt ttcacgcgcc tcggcaagcg gcgcaacgtc gcgtatggca tgccaaagcc
     1621 cttctgatgc aggagcatcg agcttcgcca tgggcgcaaa attttttagc agcgtttcaa
     1681 gcagcgcgag gcgcccttcg acggaaggcg caaagccttc aacgcgcaaa gccgtgatcg
     1741 cattctcaaa accttgcgtt gctgcgggga aagacggcag atgcgcggca ccagatacgt
     1801 cagcgggcgc gcccatcgcg gcacaaagcg cttttatcgc ggatccggga tcgaggccga
     1861 aaagtagcaa tgtcgcggac gtctccgcgc gcggcagaac ttttagtgtg atttcagttg
     1921 cgactgcgat cgttccaaat gacccggcaa ggccgcgcga gagatcatag cccgttacat
     1981 ttttgacgac ccggccgccg gccttaaatg ctttgccacg gccggagacc gccttgagcc
     2041 ccaagacaaa atcgcgcgcg gctcccgcct tgatccgacg cgggccggag agattagcca
     2101 taagactgcc gccgatcgtt ccatgccccg tgccataaag cgcggcaaaa gacatcggtt
     2161 cgaaggcaag ctcctgacca cttgctgcca agagcgcttc gatggtcgca atcggcgttc
     2221 cggctagcgc ggagaggaca agctcttcgg gttcgtaaaa gcttatgcca tcaaggcggg
     2281 aaagatcgag ccggcaaggg tttcttaccg gtctgccgag cgccgacttt gtgccgaggc
     2341 cgatgatcga aagcggcgcg tgctttgcca ccgcttcggt gaccacttcc gccacctcag
     2401 cctgatcagc ggggatgaaa tgagacatgt ttgatcaggg actcaatcga accaaggagt
     2461 ctatagcttt tttcaattca gctgcgggaa ggcttttggt aggattgccg ggcgaggccg
     2521 ctgtatatgt gcaaaagcgc gagctttgtc ccttgtacga aaatttttgt tcggcccaat
     2581 gatgaagttt gcggtcataa cgtttccata caccaccgat acagatttgg gcacagcagc
     2641 tgggcctgaa gccctgctgc gggcagggct gacaaactgg ctgcgtgaaa agggccacga
     2701 tttggccggt cctttccatg tcaaacttag cgcatgcgaa gaggcggcct ctggcgcctg
     2761 gaacaagctc ggctttgcca atgcgcatct tgcgcggctc gtctcggatg cggtgcggaa
     2821 cagggctttt ccactgatct tggagagcaa ttgctacgcc gccataggcg tttgggcggg
     2881 gctgcaaatg tctgccggga aggcgtcgca gcgcctcgcc atggtctgga tcgacgcgca
     2941 tggcgattgc aacacgccgg agaccacgtt gagtggcatg ctgagcggca tgccagtcgc
     3001 cattgcgatc ggcctatgcc tcgaccggtt ccgcaaacag tcgggtctct atgctccgat
     3061 agaccagcgc gacaccgtca tggtttgcgt gcgcgcaaat gaccctcttg agcaggagct
     3121 gatcgatcaa cttgaaattg aaatggtgcc ggttttagac atcaagggcg attgccgaaa
     3181 acttcgcgcc gcgatggagc acttgagtcg gagtgttaac gcgatctacg tgcacttcga
     3241 tgtcgacgcg ctcgacgaaa ctgaagtggc ctcgatgtgg ctcacggcgc ccgatggtcc
     3301 aacggcgcga gaattggcgg cggcgatgga gatcgttatg gcctttccaa aaatcgccgc
     3361 ctttggggtt agcgacatta atccggagag ggacgttgag ggtcagatgg tgcatgcggc
     3421 gctgactgtc attaaagggg gtattgcggc gttgccgggt aaatcgactc tccggcacag
     3481 tggctccaga gggcgcgcgc cagcttcaag agaaaaaggg aaacggcaat gaatttgagc
     3541 aagcatggtc atcatcatga aggaacccag gagcggtcta cccgccctga cttgacgcga
     3601 ttgcatcata cgccgctttt ttgggttgcc cccatcttga tgctggtttc catcactgtc
     3661 tacgtgatgt cattatcaga tgatttcgca gaaccaggaa ggaccggaca cggttgagtt
     3721 ggcccctgtt ggttcagagg ttcgcaaaag tgcagggcga aaacgggaaa aagtcctcgt
     3781 caa
//
LOCUS       DATMPU010000076         3670 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_676345,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000076 DATMPU010000000
VERSION     DATMPU010000076.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 3670)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3670)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3670
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_676345"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..1890)
                     /gene="shc"
                     /locus_tag="VNY06_01750"
                     /pseudo
     CDS             complement(1..1890)
                     /gene="shc"
                     /locus_tag="VNY06_01750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020177153.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0051007 - squalene-hopene cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0019746 - hopanoid biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="squalene--hopene cyclase"
     gene            complement(2062..2376)
                     /locus_tag="VNY06_01755"
     CDS             complement(2062..2376)
                     /locus_tag="VNY06_01755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX13597.1"
                     /translation="MVDVKHFRDASEDIAEDFGALREEVAKLSASVSELVRAQASSAN
                     AVMEGTESARQRLSSGAAEAQNRLRGVGADIEATIERNPLAAVLTALSAGFADRNDER
                     RT"
     gene            complement(3129..3557)
                     /locus_tag="VNY06_01760"
     CDS             complement(3129..3557)
                     /locus_tag="VNY06_01760"
                     /inference="COORDINATES: protein motif:HMM:TIGR02284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PA2169 family four-helix-bundle protein"
                     /protein_id="HWX13598.1"
                     /translation="MDPKKETIKSSITGMDDFVVLTECERGEDAAKRAYEAALNEDLP
                     ADIKALVQRQYEGVKTITSVSAICGTNTPTSEAERAELQTVSAAPNGGGVLFMRSVLE
                     LRNVSLFAKCSVALPVDEILNRASSTRAQKGNLRASVTIY"
BASE COUNT          762 a         1123 c          995 g          790 t
ORIGIN      
        1 atgatagcgg agaaaaaaca cccggggaaa accggtcgcg gtaaaacgct cttccttcca
       61 aaatccgtcc tccccctggg tcttcgcaag ataatttatc cccgacagaa ccgtagcatc
      121 gtcaaccata ccggcggcca tcaatcctag caatgcccat gccgtttgcg atggcgtgct
      181 cggtgcgcgc tggtagccag cataatcgag cgcataactc tcgccgcctt cgccccagcc
      241 gccgtctttg ttctggatgc ttttcagcca ggcggcggct ttttgcacct ccgcgcttcg
      301 cggatcgcgt tggaccgcat taaacgcgca gagcaccgac caggttccgt agatgtaatt
      361 cattccccac cggccaaacc agctgccatc cttctcctgg gtgcgcacga gatagtcgaa
      421 gccgcgcgcg gccgctttgt tgcccgccgg cgtctcgccg agctggccca gcatggaaag
      481 acaacgcgcc gtcacatccg ccgtcggcgg atcgagcaag gcaccgtgat cggcgaaagg
      541 aatataattc aaataatcat aatcattatc agcatcgaag gcgccccagc cgccattggc
      601 gctttgcagg ccttcgatcc attctcgcgc gcgcgaaatc gcgtccttgt agtctggtcc
      661 agggcctgcc gctccatggc ttgccgcgct ggccatcgcc attgcgacga cagcggtgtc
      721 gtcgacatcg ggataatagg cattggcata ttgaaatgcc cagccgcctg gccgcacatg
      781 gggcttttgc acggcccaat cgccggcgac accggtaact tgcagaggtt tgagccagga
      841 aagcgcggcg cgaactggcg gcgccactgc ttccccacca gcttccatca gcgcgtgaca
      901 ggcaagcccc gtgtcccaca ccggcgagac gcagggctgg caataagcct cgtcttcctc
      961 gacgacgagc agcttgtcga tcgcattaag gacctcgacc attttcggat cttgccttcc
     1021 atagccgagc gcgtcgaaca tcatcgccga gtaggccatc gccggataga tcgcgccgag
     1081 cccatcctcg ccattcaggc gttcttcgac gaagccaacc gccttgccga tcgcgcgctt
     1141 gcgcaagctt ttggggaaat aaggctctga ccagcgcaaa acgctgtcca ctccggcaaa
     1201 cagcgtcgtc cagggaaatt tttggtgggg cgcgccgggc caatggcgaa ccctgtccgg
     1261 cggttcgacg aaaagttcgc cgatggtgat attgcgcgga tttcgcgccc gcggcttcac
     1321 cgcgttcaca accatcagcg gaatgagaag ggtgcggccc cagtaggaga ttttggtgac
     1381 atgaaacgga aaccagcgtg ccaacagcat gatctcgacc ggcatgaccg gcacgctgcg
     1441 ccaaggtacc gcgccgaaaa gcgctagtaa ggcacgcgta aaaacatttg tgtttgccgc
     1501 tccaccatgc gccaggatgg ccgcgcgggc acgccgcatg tgatccgcct cgggcaaatc
     1561 gcccacggcc ttcagggcga aatacgtctt gacgctgctg ctgacgttga aggggccatc
     1621 ggtgaacagc ggccaaccgt catgcgtggc ctgttagcgc cgcagatagc ttgcgagctt
     1681 ggcttcgagc ccggcatcgg ctggctcgcc gagaaaatgc ttgaaaagaa tatattccgc
     1741 cgggatcgaa acatccgctt cgagctcaaa gacgaaatgt ccgtcctcgc gctgctcggc
     1801 caaaagcgct tgcgttgacc gggaaaccgc cgtttcgatc tcggcgaggc ccgggtccgc
     1861 gggctccgaa gcggatgacc ggaaagacac acgatttgac accacgagcg gtctactcgt
     1921 tccgcctgcg attagtcgca gcgtaattcc agagaacgtc aagatgagcc atgcttaacg
     1981 aaccttgtct tactaaacca agacgttaaa cgcaccgacg aatggctcgg cggctgcaag
     2041 ccgcctcatc agtgaagtca tctacgtgcg ccgctcatca ttccgatcag caaaacctgc
     2101 ggacagtgcc gtcagcaccg ctgccaaggg gttgcgctca atcgtggcct caatatccgc
     2161 gcccacgccg cggagccgat tttgcgcctc ggcagcgcca ctggaaagcc gctgccgggc
     2221 actttcggtc ccctccatga cggcattggc actcgaagct tgtgcacgca caagctcgga
     2281 gacggatgca gatagtttag cgacctcctc ccgcagcgcg ccgaagtcct cggcgatgtc
     2341 ttccgaagcg tccctaaagt gcttaacgtc gaccatactg ctctatccct tttgcgccac
     2401 ctatgactcc ggcggtcgcg gtttcctggt tgtcacgacc agtacgaaac tcatttcgat
     2461 atgactggtt cccggagcat ccaaagcatt tgcggtaggt ttatccccgt tctttgaact
     2521 cgagctgtca cgagcattgg gctcgcttga gcgatagtgc cgagaccccc gcttcttgct
     2581 tgtgcgtcca tcaacccggg gcgcgattgg ctcctccgat accccattct gaaaacgcca
     2641 aacaattgga ccgcaaccta aacgccgcgc cacttccata gttcctggca tttcaccgtc
     2701 atgtgatttg ctccaaccca tgcccctttt ttcgaatgtt gatcggtcaa agctgctttc
     2761 cgcgttccgg cgcattgcca agcatcgccg tgagcctttg gatgggtgct tagtatggcc
     2821 tgtaattgca tctttcacat tcctttggat ttttggctac gttcacatcc atgatcatgc
     2881 aagcgctccg aaccccttta gcggaaacct cgttctcgcc gcgcttcacc aagagagcgg
     2941 cgctggcgac gatatgctgt gctttggatt ttgattcgtt gggcgtccat ggggtacgca
     3001 gatcccagcc ataatgatga aggccgttac gccggtggca gtcattagaa tgtcaacgaa
     3061 ctgcaagtag tgagcaaatt ccgcgagtgc ctcctttcgc atcaacccag gcgttcttcc
     3121 aagatcaatt agtaaatcgt aacggaggca cgaaggtttc ctttttgagc gcgtgtgctt
     3181 gaggcgcggt tcaaaatttc gtcaaccggc aatgcgacgg agcacttggc gaaaagagac
     3241 acattacgca gctcgagcac gctgcgcata aaaagtacgc cgccaccatt gggggcagcg
     3301 gatacagttt gcaactcggc acgctctgct tcagaggtgg gcgtgttggt tccgcagatc
     3361 gcggatacgc tcgtgattgt cttcacccct tcgtactgcc gttgaacaag cgcttttata
     3421 tcggcaggta ggtcctcgtt tagggctgct tcataggcgc gttttgcagc gtcttcgccg
     3481 cgctcgcatt ccgtaagcac cacaaagtca tccatacccg tgatcgagga cttgattgtc
     3541 tcttttttgg gatccaaacc ttcgtcttca gacgaagaga ggcttgagcc gttttttggt
     3601 catagactga ctccgtaaaa tgggcgtctc cgggggcaaa cgcccatttt acggagtcag
     3661 tctatgagct
//
LOCUS       DATMPU010000077         5935 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_1607787,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000077 DATMPU010000000
VERSION     DATMPU010000077.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 5935)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5935)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5935
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1607787"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(177..1055)
                     /locus_tag="VNY06_01765"
     CDS             complement(177..1055)
                     /locus_tag="VNY06_01765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012386188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HWX13599.1"
                     /translation="MIYDIRHLTLYEYGSTVTFSHCALRLLPTDGPGQTVLSSELRIT
                     PEPAQTVERTCFFGNRLTILTIGTAHRELSVEVTASVEIDRRDPEGPKESQAFESVRE
                     EAFVSASLDRSSPAHFLHASRLVPRFNPVTDYARESFPARRPVLDGAKELMRRIKADF
                     KYDTKATVVSTPISEAFSKRHGVCQDFAHIMIAGLRGLGLPAAYISGYIRTIPRPGKK
                     RLEGADAMHAWVSLWCGKSHGWVGLDPTNAMMIGNDHIVLAKGRDYADISPVAGIVLG
                     SREQDVDVRVDVVPRV"
     gene            complement(1052..1759)
                     /locus_tag="VNY06_01770"
     CDS             complement(1052..1759)
                     /locus_tag="VNY06_01770"
                     /inference="COORDINATES: protein motif:HMM:NF016086.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-E domain-containing protein"
                     /protein_id="HWX13600.1"
                     /translation="MRLSAFPCPRCTPCASFIRERLSTDTWRLIGDLNQNLDADWRTA
                     LTEPEVFERADAALRTIAAISGLAQENMNRGAGWRMFDAGRRIERSINTCRLARHFAS
                     ADAPADDLDVLLDLVDSQITFRSRYLLGLALNPVRDMVVLDSFNPRSVAFQVERLDEH
                     LATLPLLSDDGMLEAPRRLILQLAAEVATATARCLNNEKILGFEQSLLALADAIAARY
                     FLQGPHVARAEESSALA"
     gene            complement(1669..2754)
                     /locus_tag="VNY06_01775"
     CDS             complement(1669..2754)
                     /locus_tag="VNY06_01775"
                     /inference="COORDINATES: protein motif:HMM:NF025761.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circularly permuted type 2 ATP-grasp protein"
                     /protein_id="HWX13601.1"
                     /translation="MTREGKVHVRTIAGLKRADVIWRRIDSDFADSLELNSHSQLGVP
                     GLVDALREGGVVIANALGSGVLEVPVMMSFMPRLCQTLLGKELRLPNIATWWCGQNKA
                     RQVVVEDMDELAIAGAFGNPMPGFANNQPLIGAAFTAEEKEQLKAAITERGVDFVGQE
                     VVNLSTTPVWHDGRLLPRPFVLRVYAARTSKGWMIMPGGFCRISGRTDARAVAMGEGV
                     QSADVWLLADKPVAMVSLLPTDETVRIRRIMGTLPSRAADNLFWLGRYLERSEATLRL
                     VRCLASRVVESDTVTAKPRSAVSKLKSLLIAWNAAPALPVSGTMTLAAIALHSEEEYG
                     SALSLVRDARRALRSYGSGSRQILGGS"
     gene            complement(2654..3568)
                     /locus_tag="VNY06_01780"
     CDS             complement(2654..3568)
                     /locus_tag="VNY06_01780"
                     /inference="COORDINATES: protein motif:HMM:NF016090.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circularly permuted type 2 ATP-grasp protein"
                     /protein_id="HWX13602.1"
                     /translation="MEFGISTKPKADFDAAARITELTSGYAPLPGIPDEFIGPDGRPR
                     AHWMRFLFALMELSAEGIIRRFATADRHMRDIGVSYRAYGDTSERSWPLSHVPLLIEA
                     SEWREIAQGIEERAQLLELILADIYGEGRLIASGDLPATAVTGPEFLYPLHGLTPPGG
                     KFLHIYAADIGRGPDGRWWVLGDRAQAPSGAGYALANRLVLARAFPDLYRDINVERLA
                     PFFEAFRLGLASMAQRSDPRICLLTPGPFNQTYFEQAYLARYLGFLLVEGGDFDDARG
                     QGACPHDRRAQARRCHLAAHRQRFRRFT"
     gene            complement(3569..4117)
                     /gene="mog"
                     /locus_tag="VNY06_01785"
     CDS             complement(3569..4117)
                     /gene="mog"
                     /locus_tag="VNY06_01785"
                     /EC_number="2.7.7.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020173526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin adenylyltransferase"
                     /protein_id="HWX13603.1"
                     /translation="MAENIRRAAIIGVVTASDRASIGIYEDQGGPAIIAYLERILKTP
                     FRVVRKITPDGFESVRDTLIELADEHHCDLILTTGGTGPSPRDLTPEATEAVAPRVLP
                     GFGELMRQESLKQVPTAILSRQIAAHRGPCLIINLPGKPSSIETCLNSVFPAVPYCLD
                     LIGAAYIETDAAILKSFRPKQA"
     gene            complement(4446..4904)
                     /locus_tag="VNY06_01790"
     CDS             complement(4446..4904)
                     /locus_tag="VNY06_01790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015932376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1178 family protein"
                     /protein_id="HWX13604.1"
                     /translation="MIRYALRCVRSHEFESWFQSGEVFDAQAKAGLVACPHCGATDVN
                     KAVMAPAVASRGSIEPAPSAANANTQAAVALLDRHDREMQAMIHAFRKRVFGAAEDVG
                     PRFAEEARKIHDGLVPERPIHGQANYEDARALIEEGMAILPLPPALDDFN"
     gene            complement(4901..5167)
                     /gene="grxC"
                     /locus_tag="VNY06_01795"
     CDS             complement(4901..5167)
                     /gene="grxC"
                     /locus_tag="VNY06_01795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012592642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HWX13605.1"
                     /translation="MPDVTIYTTASCPYCIRAKALLRKKNVSFREIALDDDPEARREM
                     AAQAHGRRTVPQIFFNGVHIGDSDELHELDRTGALDKLVAGLAR"
     gene            5427..5813
                     /locus_tag="VNY06_01800"
     CDS             5427..5813
                     /locus_tag="VNY06_01800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016921947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2852 domain-containing protein"
                     /protein_id="HWX13606.1"
                     /translation="MEVLAMVLGFIVFWPIGLAVLGWKIWQKKSGYQGDIISFGREKW
                     ANRAQWGFSTNQAHGFGGGAASTGNLAFDEWRAAELARLEEEYQRLAAAEREFAEYMN
                     NLRRARDREEFERFMNERRRRQEGPA"
BASE COUNT         1235 a         1701 c         1800 g         1199 t
ORIGIN      
        1 gccgacgcca tttggggtgc gaaaatgttg ttgcggcgca aactcattga caacaagaag
       61 ggaaaacaat cgctgccgct atcgtcacat gggggcgtga gcgacagccg gggctaatgc
      121 gctttcgctg ttctcatgga ctgtccatgc catccgattc gccggatatt ctcgtgtcac
      181 actctcggca caacatcgac ccgcacatcg acatcctgct cgcgtgagcc gaggacaatt
      241 ccggcgactg gggatatatc cgcatagtcg cggcctttcg ccaagacaat gtggtcgttg
      301 ccgatcatca tcgcgttggt cgggtcaagt ccaacccagc catgagattt gccgcaccag
      361 agcgataccc aagcatgcat cgcatcggca ccctcgagac gctttttgcc aggccgcgga
      421 atcgtgcgga tgtagcccga aatataggcg gcaggcaggc cgaggccacg caggcctgca
      481 atcatgatat gcgcgaagtc ttggcacacg ccgtgccgtt tgctgaaggc ttccgagatc
      541 ggcgttgaga ccactgtcgc tttcgtatca tatttgaaat cggctttgat tcgccgcatc
      601 aattcctttg caccatcgag aactggccgc ctggcgggaa aactttcccg cgcgtagtcg
      661 gtaacggggt tgaaccgcgg cacgagacgg cttgcatgta gaaaatgggc aggtgaactc
      721 ctgtcgagag aagcgctgac gaaggcttcc tcccgcacgg attcgaaggc ttgagattcc
      781 tttggccctt ctggatctct ccggtcgatt tcgacgcttg cagtaacctc cacggaaagt
      841 tcgcgatgcg ccgttccaat cgtgaggatc gtgaggcggt tgccaaaaaa acacgtccgt
      901 tcgaccgttt gcgcaggttc tggagtgatg cgcagttcgc tcgaaagcac tgtttgtccc
      961 ggaccgtcgg tcggcaagag ccgcaaggcg caatgcgaga atgttacggt cgagccatat
     1021 tcataaagcg tcagatgtcg aatatcgtag atcacgccag tgcactcgat tcttcggccc
     1081 tggcgacatg aggtccctgc aggaagtaac gcgcggcgat tgcatcggcg agagcgagaa
     1141 ggctctgctc gaagccgaga atcttttcat tgttgagaca gcgcgcggtt gctgtcgcaa
     1201 cttcggcggc aagttgcagg atcagccggc gcggcgcctc aagcatacca tcgtcgctga
     1261 gcagcggcag agtcgcgaga tgttcgtcga gccgctccac ttggaaggct accgagcggg
     1321 gattgaatga atcgagcacg accatgtcgc ggaccgggtt gagggccaga ccaagcaaat
     1381 accgcgaccg gaaggtaatt tgtgaatcaa cgagatcgag caagacatcg agatcgtccg
     1441 ctggcgcgtc ggcgcttgcg aaatggcgcg ccaagcggca ggtgttgatg ctccgctcga
     1501 tacgccgccc ggcatcgaac atgcgccatc cggcaccgcg gttcatgttt tcctgagcca
     1561 agccggagat tgccgcgatc gtccgcaagg ccgcgtcggc tcgttcgaaa acctcaggct
     1621 cggtaagcgc tgtgcgccag tcggcgtcaa ggttttgatt gaggtcgcct atgagccgcc
     1681 aagtatctgt cgagagccgc tcccgtatga acgaagcgca cggcgtgcat cgcggacaag
     1741 ggaaagcgct gagccgtatt cttcctcgct gtgcaaggca atcgcagcaa gcgtcatcgt
     1801 gcccgaaaca ggcaaggccg gcgccgcgtt ccaggcaatg agcaggctct tgagtttaga
     1861 taccgccgat cgaggtttgg ctgtcacggt gtcagattcg acgactcggc tcgcaaggca
     1921 gcggacgagc cgcaatgtcg cttccgaacg ttcgagatag cgcccgagcc agaataggtt
     1981 atccgcagct ctgctcggca atgtgcccat gatgcggcga attcgaaccg tctcgtcggt
     2041 tggcagcagg ctcaccattg cgacaggctt gtcagcgagc agccagacat cggccgattg
     2101 cacgccttcg cccatcgcca cggcgcgggc gtcggtgcgg ccggaaatgc ggcagaagcc
     2161 gcctggcatg atcatccacc ccttcgacgt gcgtgccgca tagactctta aaacgaatgg
     2221 ccgcggcagc agcctgccat cgtgccatac gggcgtcgtc gatagattca cgacctcttg
     2281 acctacgaag tccacgccgc gctctgtaat tgcggccttg agctgttcct tttcttcggc
     2341 ggtgaacgcg gctccgatga gcggctgatt attggcaaag ccgggcatcg gattcccgaa
     2401 cgcgcccgcg atcgcaagct cgtccatatc ctcgactacc acctggcgcg ccttattctg
     2461 gccgcaccac caagtcgcga tattgggcag ccgcaactcc ttacccagta aggtctggca
     2521 gagcctcggc atgaagctca tcatcaccgg gacttcaagc acgcccgacc caagcgcatt
     2581 ggcaataacg acaccgcctt cgcggagcgc atcaacaagg cccggaacgc caagctgcga
     2641 atgggagtta agttcaagtg aatcggcgaa atcgctgtcg atgcgccgcc aaatgacatc
     2701 ggcgcgcttg agcccggcga tcgtgcggac atgcaccttg ccctcgcgcg tcatcaaaat
     2761 cgcccccttc gaccaggagg aagccgaggt agcgggcaag ataggcctgc tcgaaatagg
     2821 tttggttaaa cgggccgggg gtcagaagac agatgcgcgg gtcagaccgc tgcgccatag
     2881 aggcaaggcc gaggcggaaa gcttcgaaga atggggcaag ccgctcgaca tttatgtcgc
     2941 ggtagaggtc ggggaaagcg cgcgccagga ccagcctatt ggcgagcgca tagccggcac
     3001 ctgaaggtgc ctgagcgcga tcgccaagaa cccaccagcg gccatcggga ccgcgaccaa
     3061 tatcggccgc gtaaatgtgc agaaatttgc cgcccggggg cgttaggcca tgcaacggat
     3121 aaaggaattc tgggccggtg acggcggtgg ccggcaaatc gcccgaggcg atcagccggc
     3181 cttcgccgta aatgtcggcc aagatcagct ccaggagctg ggcgcgctcc tcgattccct
     3241 gtgcgatttc gcgccactca cttgcctcga tgagtagcgg cacatgcgaa agcggccaag
     3301 agcgttcgct tgtgtctcca taggcgcggt aagatacgcc aatgtcgcgc atgtgtcggt
     3361 cggcggtggc gaagcgccgg atgatgcctt ctgcgctgag ttccatcaat gcgaagagaa
     3421 accgcatcca atgcgcccgg ggccggccat ctgggccgat gaattcatcc ggaatgccag
     3481 gcagcggcgc gtaaccagag gtgagctcag ttatgcgtgc tgccgcgtca aaatccgcct
     3541 ttggcttcgt tgatatgccg aactccattt aggcttgctt tggccggaag gattttagga
     3601 tcgccgcatc cgtctcaata taggcggcgc cgataagatc aagacaataa ggcaccgctg
     3661 gaaagaccga attgaggcag gtttcgatcg acgaaggttt gcccggaaga ttgatgatga
     3721 gacaaggccc gcggtgcgcc gcgatttggc gcgacaggat cgcggtcgga acctgcttca
     3781 agctttcctg gcgcatgagc tcgccaaagc caggcaaaac gcgcggtgcc accgcctcag
     3841 ttgcttccgg tgtcaggtcg cgcggagagg gtccggttcc gcccgtggtc aggatcaaat
     3901 cgcaatgatg ctcgtcggca agctcaatca gcgtgtcccg cacggattcg aatccgtctg
     3961 gggtaatctt ccgcacaacg cgaaaagggg ttttgaggat tctttcgaga taagcgatga
     4021 tggcaggccc gccttgatcc tcgtaaatgc cgatgctggc acggtcggaa gctgtgacga
     4081 cccctatgat cgccgcgcgc cttatattct ccgccatgcc aacccttccg ctgctttgct
     4141 cgcttgctaa gacatagcgg atcggaaggg atttttccaa ccgcttgcgt ggcccgacgc
     4201 tcgaagcttg gaagcatgcc catttttttt ctttgccgta tcaactctac caatcgtacc
     4261 aagtgtccgg ttgggccaaa aaccttacaa ggccagatac ttctagcagc ctttggctaa
     4321 ctggaatcgg gcaagaaatt ggtatttttg gcggactctt ttttggttga gccaggcttt
     4381 ctgcgggagc gtgcctattc aggccaattg tgcaggtcgg gaagatcgct gacggtgaag
     4441 ctaccctaat taaaatcatc cagcgcggga gggagaggca aaatcgccat gccttcttcg
     4501 atcaaggcac gggcatcttc ataattcgcc tggccgtgga tcggacgctc cgggacgagg
     4561 ccgtcgtgaa ttttgcgcgc ctcttcggca aaacgcgggc ctacgtcctc ggcggctccg
     4621 aagacgcgct tgcgaaaagc atggatcatt gcctgcattt cgcgatcatg ccggtcgagg
     4681 agagcgaccg cagcttgggt atttgcgttc gcggcggatg gagcgggctc tatgctcccc
     4741 cggcttgcga ccgcaggcgc catgaccgct ttgtttacgt ctgtcgcccc gcaatgcggg
     4801 caagcgacga ggccagcctt cgcttgcgcg tcaaaaacct cgccgctctg aaaccaactc
     4861 tcgaattcat ggctgcgcac gcagcgcagg gcgtatctga tcatcgcgcg aggccagcca
     4921 caagtttgtc gagtgcgccg gttcggtcaa gctcgtggag ctcatcgctg tccccgatat
     4981 gaacaccgtt aaaaaaaatc tgcggcacag tcctacgccc atgcgcctgc gccgccattt
     5041 cccggcgcgc ctcagggtcg tcgtcgagcg cgatttctcg gaatgatacg tttttcttgc
     5101 gcaacaatgc cttggcccgg atgcaataag ggcagcttgc tgttgtataa attgtgacgt
     5161 ccggcattac agttaccttt tgctcgcgcc ttatatgggg gtgtgcggca aaagattccg
     5221 cttcccgagc atcgcaacag caaaagccgt acctatattg ggagcgccag tctccgccgc
     5281 tgcgcctcgg gcgtgcggcg gaaaataagg ctgtaatgga gtttaagatg agtgctgatg
     5341 gcggtgtgtc ttgtcaatct tactcgtccc ggcgatccgg gggaggccag tggcgaggat
     5401 gtgcgccggg agaaccctgg aaagcggtgg aagtcctcgc gatggttttg ggctttatcg
     5461 tgttttggcc aatcggcctt gccgttctcg ggtggaaaat ctggcagaaa aagtccggtt
     5521 atcaaggcga catcatttcc tttgggcggg aaaaatgggc caaccgcgcg cagtggggct
     5581 tttcgacaaa tcaggcccat ggttttggcg gtggagcagc gtctacgggg aatctggctt
     5641 ttgacgaatg gcgcgcggcg gaacttgcgc ggctcgagga agagtaccag cggctcgctg
     5701 cagccgagcg ggagtttgcc gaatatatga acaatcttcg ccgcgccagg gaccgtgaag
     5761 aattcgagcg cttcatgaat gagcgcagac gccggcaaga aggtccggcc taacgttaga
     5821 gccgccgttt gcggcgaaga ccgctccccg ccaaattctc caagacaaca aggtcgtggc
     5881 cgccacaatg tgacggccac gcgcctcttt agttgcggcg gccgcgttag gagga
//
LOCUS       DATMPU010000078         4604 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_1917042,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000078 DATMPU010000000
VERSION     DATMPU010000078.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 4604)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4604)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4604
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1917042"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..311)
                     /gene="rplQ"
                     /locus_tag="VNY06_01805"
     CDS             complement(<1..311)
                     /gene="rplQ"
                     /locus_tag="VNY06_01805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012330317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HWX13607.1"
                     /translation="MYHGHAKRRFGRTHEHRKAMFSNLCQALIKHEQIVTTLPKAKDL
                     RPVVEKLVTLGKRGDLHARRQAIAQIRDTALVNKLFAVLGPRYKDRHGGYTRVLKAGF
                     R"
     gene            complement(403..1419)
                     /locus_tag="VNY06_01810"
     CDS             complement(403..1419)
                     /locus_tag="VNY06_01810"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020185768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HWX13608.1"
                     /translation="MIQKNWQELIKPSKLDVFAGDEPKRMAMIVAEPLERGFGLTLGN
                     ALRRILLSSLQGAAISSVHIDGVLHEFSSIPGVREDVIDIVLNIKDIALKMPGDGPKR
                     MVLRKQGPGKVTAGDISTTGDISILNPELAICTLDEGAEIRMEFTVNTGKGYIPAERN
                     RAEDAPIGLIPIDSLYSPVRKVSYQIENTREGQILDYDKLTIQVETNGSLSPEDAIAF
                     SARILQDQLNVFVNFEEPHRVEAAPSIPELAFNPALLKKVDELELSVRSANCLKNDNI
                     VYIGDLILKTEAEMLRTPNFGRKSLNEIKEVLAQMGLHLGMDVPGWPPENIDELAKRF
                     EEHY"
     gene            complement(1521..1910)
                     /gene="rpsK"
                     /locus_tag="VNY06_01815"
     CDS             complement(1521..1910)
                     /gene="rpsK"
                     /locus_tag="VNY06_01815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007603045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HWX13609.1"
                     /translation="MAKETTRVRRRERKNIASGVAHVSSTFNNTMITITDAQGNTIAW
                     SSAGTMGFKGSRKSTPYAAQMAAEDCARKAAEHGMRTLEVEVSGPGSGRESALRALQA
                     AGFTVTSIRDVTPIPHNGCRPCKRRRV"
     gene            complement(2032..2400)
                     /gene="rpsM"
                     /locus_tag="VNY06_01820"
     CDS             complement(2032..2400)
                     /gene="rpsM"
                     /locus_tag="VNY06_01820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011651425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HWX13610.1"
                     /translation="MARIAGVNIPTNKRVIVALQYIHGIGPAKAKEICGRVNIPSERR
                     VLDLTDAEVLQIREAIDRDYIVEGDLRRDVATHIKRLMDLGCYRGLRHRRQLPVRGQR
                     THTNARTRKGKAKPIAGKKK"
     gene            2716..3186
                     /locus_tag="VNY06_01825"
     CDS             2716..3186
                     /locus_tag="VNY06_01825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX13611.1"
                     /translation="MSTPEETNKRPKKPPREGIPLERILIALGLFGVVILVFMGVRTL
                     TERVKLDAVPIAGEWQAQRESWRIEFKPDNTIVSSTGPSRPDASQPWTSGKGKYKVDY
                     FGTLWVTLDDGKVFKATLSPEMPNRFDLIEMATEGVTVFERVAQPQPNPPQSPK"
     gene            complement(3224..3472)
                     /locus_tag="VNY06_01830"
     CDS             complement(3224..3472)
                     /locus_tag="VNY06_01830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013950526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX13612.1"
                     /translation="MNRLLIMAAIAALIFLPAAAHAQERLGDGALGALTGALVGGPVG
                     LVAGGVVGYTAGPAIASSWGLRGHRHYHRARYRRHHPQ"
     gene            complement(3845..>4604)
                     /locus_tag="VNY06_01835"
     CDS             complement(3845..>4604)
                     /locus_tag="VNY06_01835"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012385643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HWX13613.1"
                     /translation="ALMAASLANGHTILINAAREPEVVDLAACLNKMGARIKDAGQSI
                     VEIEGVTSLAGARHIVMPDRIEAGTYAMAAVMAGGDVLLEGANSSLLESAIEAIARCG
                     AEFSETNTGLRVKRNGSGLAAVDVVTKPFPGFPTDLQAQFMALMTRAKGTSRITETIF
                     ENRFIHVQELVRLGAHIKLEGDSAVVEGVDELQGAPVMATDLRASMSLVIAALAANGE
                     TIVNRVYHLDRGFEHIEEKLGACGAVIQRLSDRS"
BASE COUNT          985 a         1395 c         1237 g          987 t
ORIGIN      
        1 tagcggaagc ctgccttcaa aaccctcgtg tagccaccgt gccgatcctt gtagcgcggg
       61 ccaagcacgg caaagagttt gttcacaaga gccgtatctc tgatctgcgc aatggcctgc
      121 cgccgcgcat gcagatcacc gcgctttcca agcgtcacga gtttttcgac gaccggccgg
      181 agatcctttg cctttggcaa ggtggtcacg atctgctcat gcttgatcag cgcttggcac
      241 aaatttgaaa acatcgcctt gcgatgctca tgcgtgcggc cgaagcggcg cttggcgtgt
      301 ccatgataca tggcgctctc cattatctcg cggccgccgt gccgaggtac ggccgctctc
      361 gttagatctc attctccatt gcacgagaca aggcaaatag gatcagtaat gctcctcaaa
      421 ccgcttggcg agctcgtcaa tgttttccgg gggccaaccc gggacatcca tgccgagatg
      481 caaacccatc tgggcaagga cttccttgat ctcgttgagc gacttgcggc caaaattcgg
      541 cgtgcgaagc atttccgctt ccgtcttcaa aatgagatcg ccgatgtaga cgatgttatc
      601 gttcttgagg caatttgccg agcgcacgga gagttcgagc tcatcgacct ttttgagaag
      661 cgcaggattg aatgcaagct caggaatcga aggcgccgcc tcgacgcggt gcggctcctc
      721 gaagttgacg aagacattga gctggtcctg aagaatgcgg gcggaaaaag caatcgcgtc
      781 ttcgggcgaa agcgatccgt tggtttctac ctggatggtc agcttatcgt aatcgagaat
      841 ttgtccttcg cgcgtgttct cgatctgata gcttaccttc ctgaccggcg agtaaagact
      901 atcaatcggg atcaatccga ttggcgcatc ttctgccctg ttacgttcag ccggaatata
      961 acccttgccg gtattgaccg taaactccat gcggatttcg gccccttcat caagggtgca
     1021 aatggcgagt tcagggttaa ggatggaaat gtcgccggtc gtgctgatat cgccggcagt
     1081 gaccttgccg gggccttgtt tccgcaaaac catccgcttt ggaccgtcgc ccggcatttt
     1141 cagggcgatg tccttgatat tgagcacgat gtcgatcacg tcctcacgta cgccagggat
     1201 cgacgaaaac tcgtgcaata cgccatcgat atgtaccgaa ctaattgccg cgccttgcag
     1261 cgaagacaac aaaatgcggc gtagcgcatt gccgagggtc aggccaaagc cgcgctcgag
     1321 cggctcggcc acgatcatgg ccatgcgctt tggttcgtca ccggcaaaca catcgagttt
     1381 gcttggcttg ataagctcct gccaattctt ctgaatcacg accggacctt tcctttatgc
     1441 gatttaacgg acgccgctgc cccgcgcccg acgccgcgcc ttttgggcga gaagtgttgg
     1501 gtaatttccc tcagaggagt ctacactcga cgccgtttgc acggccggca gccattgtgc
     1561 ggaataggcg taacgtcccg gattgatgtc accgtgaatc ccgccgcttg cagcgcccgc
     1621 aaagccgatt cccgccccga gcctggcccc gaaacctcga cctcgagcgt gcgcattcca
     1681 tgttcggcag cttttctcgc gcagtcctcg gctgccatct gcgccgcata gggcgtcgat
     1741 ttgcgcgatc ccttgaaccc cattgtcccc gccgacgacc aggcaattgt gttgccctgc
     1801 gcgtcggtga tcgtaatcat tgtgttatta aaagtcgaac tgacgtgggc gacgccggat
     1861 gcaatgtttt tgcgctcgcg gcggcgaacg cgtgtcgtct cttttgccat tgattcattc
     1921 cttcaatcgc gcccgtcatt ccaggcgctc gggagatagt agtaatgacc gcagcggacg
     1981 cctgaccggc tctccttaga gacaaccgcc aaaatctgct cctcactgac gctacttttt
     2041 cttccccgcg atcggcttgg ccttaccctt gcgcgtgcgg gcgttagtat gggtgcgctg
     2101 cccgcgaacc ggcaattgcc ggcggtgacg aagcccgcga tagcagccaa gatccatcag
     2161 ccgcttgatg tgcgtcgcaa cgtcgcggcg aaggtcccct tcgacgatgt aatcgcgatc
     2221 aatcgcctcc cgaatttgca ggacttccgc atccgtcaaa tcgagcacgc ggcgctcgga
     2281 cggaatattg acacgcccgc aaatttcctt ggctttcgcc gggccaatgc cgtgaatata
     2341 ttgaagcgcg acgattaccc gcttgttggt cggaatattt acgcctgcta tacgagccaa
     2401 agccctgttc tccatgtgcg ccgcgcagcg gccaaagctt tgcggcggaa caattcgtcc
     2461 tgcgtccagt ggagactggc cccttgcggg attcctggca acagaagatc ccagcagcca
     2521 acggccccca agaatcctcg gttcaggcag gaattggtct cgttagccga tcgcgcgcgc
     2581 gccgtcaaca ggcccgggtt acccgggaac cgcggcttgg tccgcgcctc ccgtttgcgt
     2641 tgctcttgcc agccatcaat ctgcgtctat aactaccttg ctgctcgaac acccaggctc
     2701 agaaaagagg ttgcgatgag tacgccagag gaaacgaata aaaggccaaa gaaaccgcca
     2761 agagagggga taccgctcga acggatcctg atagctctcg gcctgtttgg cgtcgtcatc
     2821 ctcgtcttca tgggagttcg cactttgaca gaaagggtca aactcgacgc cgtcccaatc
     2881 gccggcgaat ggcaagctca acgcgaatcc tggcgcatcg agtttaagcc ggacaataca
     2941 attgtgagtt caaccggtcc ttctcggccg gatgcctcgc agccatggac ctcaggaaag
     3001 ggcaaatata aggtggatta cttcggtact ctgtgggtaa ctttggacga tggcaaggtc
     3061 tttaaggcga cgctgtcacc cgagatgccg aaccggttcg accttattga gatggctacg
     3121 gaaggggtaa ccgttttcga gcgggtcgcg cagccacaac caaatccgcc tcaatctcca
     3181 aaatagactc gctccaaaat agactcgccg caagtcgcgc gccttattgc gggtgatgac
     3241 gccggtaacg ggcccggtga taatgcctgt gccctctaag cccccaactc gaagcaatag
     3301 ccggcccggc cgtatagcca acgaccccgc ccgccaccaa accgaccggg cctcccacca
     3361 aagcgcctgt cagcgcgccc aaggcgccat cgccaagccg ttcctgcgcg tgtgcggcag
     3421 cagggagaaa aatcaaagcc gcaattgcgg ccataatcaa aagcctgttc atagcgtcaa
     3481 tgttcccccg aaggctacgg cgtcccacaa ccacgccaga cacatcatcc aatctgcagc
     3541 cctgccccag tatcatgaaa aaaataggga attgccgagg tctttgcggg cttcgcgctc
     3601 cgagcgccac agtccgcagg gatcgaagtc agatcgttaa ctgccgattg acctttcgag
     3661 gtgagcaaac ccgaccagaa tcctgacttc agacctcagc gccttagcgg catatcgtgc
     3721 gacggattga gagcccttag gctgaaaaca acgcgtgaca atccacagcg tcgactgtcc
     3781 agcacggtta aagaataacg gcacaagaac tccgcttctt tagtcgaatt tgcaccgcag
     3841 tacatcagct ccggtcggaa agccgctgga ttactgctcc gcaggcccca agcttttcct
     3901 cgatatgctc gaagccgcga tcgagatgat agaccctatt gacgatcgtt tcgccgttgg
     3961 ctgcgagcgc ggcgatgaca agagacattg atgcgcgcaa atccgtcgcc atgacgggtg
     4021 caccttggag ctcgtcaacc ccttcgacaa ccgcagaatc accctcaagc tttatatgtg
     4081 cacctagccg gaccagctcc tggacgtgaa taaagcggtt ctcgaaaatc gtttcggtaa
     4141 tccgcgaagt gcctttggcc ctggtcatca gggccatgaa ctgggcttgc aaatccgtcg
     4201 gaaatccggg aaatggcttg gtgacgacgt caactgccgc aagacccgag ccgttgcgct
     4261 ttacgcgcag ccccgtattc gtctccgaga attcagctcc acagcgcgcg atggcttcaa
     4321 tcgcgctttc caataggctt gagttcgcgc cttccagcaa tacgtcgccg cccgccatga
     4381 cggctgccat tgcataggtg ccagcctcaa tgcggtccgg catgacaatg tgccgcgcac
     4441 cggcaaggct ggtaacacct tcgatctcga caatcgattg tcccgcgtcc ttgatccttg
     4501 cgcccatttt attgagacat gcggcaagat cgacaacctc cggctccctt gccgcattga
     4561 taagaatcgt gtggccattg gcgagcgaag ctgccatgag cgcc
//
LOCUS       DATMPU010000079         4431 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_368011,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000079 DATMPU010000000
VERSION     DATMPU010000079.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 4431)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4431)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4431
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_368011"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            585..761
                     /locus_tag="VNY06_01840"
     CDS             585..761
                     /locus_tag="VNY06_01840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018267802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX13614.1"
                     /translation="MNVPTGGSLSIKTVCIFAAIGAVLGGADGATAAGFQEAVIFVII
                     GGMIGGIIGVFVGR"
     gene            829..1047
                     /locus_tag="VNY06_01845"
     CDS             829..1047
                     /locus_tag="VNY06_01845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007564690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1192 domain-containing protein"
                     /protein_id="HWX13615.1"
                     /translation="MAEDDDGVFGAPPRKAGTSHEIGQNVDDLSAQEITERIAILRAE
                     IARLEEARRAKQASLGAADAFFKFDRSR"
     gene            1086..2021
                     /locus_tag="VNY06_01850"
     CDS             1086..2021
                     /locus_tag="VNY06_01850"
                     /inference="COORDINATES: protein motif:HMM:NF021389.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2090 domain-containing protein"
                     /protein_id="HWX13616.1"
                     /translation="MSQDMRIGYNKPLYILPFDHRSSFEKGLYGWSGVLSAEQTEQIA
                     RAKDVIYDAFKFALRAGVAKDRTGILVDEQFGARILRDANANGFITAMPAEKSGQTEF
                     QFEFGAQFAGHIEQFKPTFVKVLVRYNVEDDEATNRRQAARLKELGDYCHSHDYYFMF
                     ELLVPATHEQMDRFEGDQNLYDRELRPSLMVSAIKALQNAGVEPDVWKIEGLDRREDC
                     RKLADAARCDGRDEVECIILGRGSNEQKVAEWLRTAAGVPGFIGFAVGRTSFWESLVA
                     WRDAKISREQAVEDIAGRYLEWVKLFEGQRHNGWT"
     gene            complement(2168..2722)
                     /locus_tag="VNY06_01855"
     CDS             complement(2168..2722)
                     /locus_tag="VNY06_01855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX13617.1"
                     /translation="MAKTKRQNGGDFDAILSGFAQQPSKKAPAYQARRPAPPAQSKPI
                     SGAPRSENPVRRLFKNISFPWPTKISGFMGNPGRRPQDAYDETVAETKPAPRVDSPQS
                     ETPQATKIDPPKPQKTEDEAIAEELGLSPDLGAIDLKRIRRDFAKKKHPDRFEPPERS
                     GAERRMSIANMLIDELMRQSRSHC"
     gene            2990..3565
                     /locus_tag="VNY06_01860"
     CDS             2990..3565
                     /locus_tag="VNY06_01860"
                     /inference="COORDINATES: protein motif:HMM:NF019430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spy/CpxP family protein refolding chaperone"
                     /protein_id="HWX13618.1"
                     /translation="MKPGLTVALTMTAIISTAIALATLSGRADPAAFPSPTQKHEFSD
                     ADRAAFLDARIAALHAGLKLSPEQEKLWPAVETALRTAGKSAMDRYQKFKNEPADRNL
                     IDRIRQRGENAVARGQNIEAIADAAAPLYATLNEEQKHRLPVLMHGLGPHFFHHHFAM
                     MGHDRENWRGHWDEHGGIEPEGGGQPGQDVR"
     gene            3712..4299
                     /locus_tag="VNY06_01865"
     CDS             3712..4299
                     /locus_tag="VNY06_01865"
                     /inference="COORDINATES: protein motif:HMM:NF013786.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HWX13619.1"
                     /translation="MGNTLAESEKERADRFLRPEDRALSIVTRGSLRCLLSEATGIAP
                     DKILLAEGPFGKPYLCGLRGPHFNVSHSGSFALIGLSDSRPIGVDIERMRVTGDELNV
                     ARSYFSATEYRALQGLWKDARLSSFFKIWTCKEAVLKACGAGISEHLKDFSVELTTDG
                     FAVHPEPHWFAPALASVSAKPVEVPPGYVGCCALA"
BASE COUNT          980 a         1191 c         1292 g          968 t
ORIGIN      
        1 tgacgtctta gggtcagcgc cccgatcgtt gcgaaagcgg caagtggcac cgcgcaggaa
       61 gagtcaagac tgaaggcaac gcttagcaat atgagcgagc cgatccagaa cagggggcgc
      121 caatcggaac caagcgagaa ccgcgccatt tttgcttctc cttatcctga cagccaggcg
      181 atttcagcac cttcccgggc cctaggtcaa gccgcaacga ttttgtcggc gacaaatcat
      241 agccgaacgc tgatgatggt ttgtcggaca gaaaacggga aagcgcgcaa ttggtctgga
      301 acaattttcc aataaggtca ttattgagct taaggtcggt gaactcaaag aagccgcgct
      361 ctatctctta agccgagagg ctttacccgc gccgacgttg gctgacgaag aactcgggat
      421 aaaggcgaac ttcccgaaaa ccgcaaaacg atgagctccc tgaggcctgc gcgtggttcg
      481 cgcaagacgt gaatagcgct ggagccaagg ccgcgcggcg caaattgaaa ggacgcgttt
      541 gcgtgaaaaa gcaggtggtc tcaggcaaat gagtgggtac gctcatgaat gttccaacag
      601 gcggctctct gtcaatcaaa actgtctgca tattcgccgc catcggcgcg gttcttggcg
      661 gggctgacgg agcgaccgcc gcaggctttc aagaagccgt aatattcgtc ataatcggcg
      721 gaatgattgg cggaataatc ggcgtttttg tcggcaggta agccgccgaa acactctttg
      781 gctcctttcc aaagatgtgt ctctgacggc aaaaagaggc ggtttgctat ggcggaagat
      841 gatgatgggg tttttggcgc accgccacgc aaggcgggga catcccatga gatcggacaa
      901 aatgtcgacg acctttccgc gcaagagatc accgagcgca ttgcgatctt gcgggctgaa
      961 attgcgcgcc tcgaggaggc gcgaagagct aaacaagcgt ctctcggcgc tgccgatgca
     1021 tttttcaagt tcgatcggtc acgctgacgc gatcaaggct ttgtctaatt cgcctcgaaa
     1081 acaaaatgag ccaagacatg cggatcggtt ataacaagcc tctctacatc ctgccctttg
     1141 accatcgcag ctccttcgaa aaaggcctct atgggtggag tggagtcctg agcgcggagc
     1201 aaaccgagca gatcgcccgg gccaaagacg tcatctatga cgcatttaag tttgctcttc
     1261 gggccggagt ggcgaaggac cggactggca tattggtcga cgagcaattt ggtgcgcgta
     1321 tcctgcgcga tgcgaacgcc aatggcttca tcaccgcgat gccggcggaa aagagcgggc
     1381 aaaccgaatt tcaattcgag ttcggcgcgc aattcgccgg acatatcgag caattcaagc
     1441 ccactttcgt caaagtcctg gtgcgctaca acgtcgaaga tgacgaggcc acgaatcgcc
     1501 ggcaagccgc gcgtctaaaa gaacttggcg actattgtca tagtcacgat tactatttca
     1561 tgttcgaact tctggtgcct gccacccatg agcagatgga ccgcttcgaa ggcgaccaga
     1621 acctttatga ccgcgagctg cgtccctcgt tgatggtctc ggcgataaag gcacttcaaa
     1681 atgccggcgt tgagcccgat gtctggaaga tcgaggggtt ggatcggcgc gaagattgcc
     1741 gcaaactcgc cgacgcagcg cggtgcgacg gtcgcgatga ggttgagtgc atcatcctcg
     1801 ggcgcgggtc caacgagcag aaggtcgcgg aatggttgcg caccgcggca ggcgtgccgg
     1861 gatttatcgg gtttgcggtg gggcggactt cgttttggga gtcgctcgtc gcgtggcgcg
     1921 acgccaaaat atctcgcgag caggcagtcg aggatattgc cggcagatat ctcgaatggg
     1981 tgaaactatt cgaaggccaa cgtcacaatg gctggacatg agttactgtc gagcgcttgg
     2041 gctcgacggc gagaggtcgg attcggctat ggatatctca tcgccgagaa agagaacatt
     2101 ggagcccacg gaatgaagac cttacttcgc cgccgcagga tatggactaa cttgaatgtc
     2161 gaatgctcta gcaatgggag cggctttgcc gcatcagttc atcgatgagc atattggcaa
     2221 ttgacatgcg ccgttccgca ccgctgcgct ctgggggttc gaatctgtcg ggatgtttct
     2281 tcttcgcgaa atcgcggcga atgcgcttaa gatcgatagc tccgaggtca gggcttagac
     2341 caagctcctc ggcgattgcc tcgtcttccg tcttttgggg cttgggcggg tcaatcttgg
     2401 ttgcctgcgg cgtttcactt tggggcgaat cgacccgagg ggccggcttt gtttcggcta
     2461 ctgtttcgtc ataggcgtct tgcggccgcc gtccggggtt ccccataaaa ccagaaattt
     2521 tggttggcca gggaaaggaa atatttttga acaggcggcg gacggggttt tcggaacgtg
     2581 gcgcgccgct aatgggtttg ctctgagccg gcggcgctgg ccgccgagcc tgataggcgg
     2641 gcgctttttt gctcggttgc tgggcaaatc cggaaagaat agcgtcgaag tcgccaccgt
     2701 tttgtcgctt agttttcgcc atcgccggag cgggcctcca tcaacgcccg gttgtccacc
     2761 cgcggcacta tgcccgcgtg gagttgatcc gcggttaatc caatgacgcc atttgcagca
     2821 aaacgtctgt ggccgcgtgc agaatggttt cttaaagata tgccaacatg ataaaaggtt
     2881 cacttgaagc catctcagac cttctctact cctcgcagca gacgggccag atccctaggg
     2941 caccgcaatc ggtgacgcgg atttgcaacg atcaacatgg agtgcgccaa tgaaaccagg
     3001 tctaactgtt gccttgacaa tgacggccat tatcagcacc gccatcgccc ttgccacctt
     3061 gagcggccgg gcggaccccg ccgcctttcc ttctcccacg caaaagcatg agttctcgga
     3121 tgccgaccgt gctgcattcc tcgatgcgcg aatcgctgcc ctccatgctg gtttgaaact
     3181 gtcgccggag caagaaaaac tctggcctgc cgttgaaacc gcgctgcgca ccgctggcaa
     3241 gagcgccatg gacagatatc agaaattcaa aaacgagccc gcagatagaa acctcattga
     3301 tcgtatccgg cagcgcggcg aaaacgcggt ggcgaggggc caaaatattg aggcaatcgc
     3361 agatgcagct gcgccgcttt atgccacact gaacgaagag caaaaacacc ggctgccggt
     3421 gctcatgcat gggctcggac cgcatttctt ccatcatcac ttcgccatga tgggtcatga
     3481 ccgcgagaac tggcgcggcc attgggatga gcacggtggc attgaaccgg aaggcggtgg
     3541 tcaaccagga caagacgtcc gctgataaat accagcacaa taaattccgc atcgcgactc
     3601 aataccgatg acggtcttgg gctgcccctc aaacggctac gtttgtcagg tagcgcgggc
     3661 gtagaacttt ggcttgctag cccttgcagc acaggcgcga tcgcggagct attggggaat
     3721 acgcttgctg aaagcgaaaa ggagcgggca gatcgttttc ttcgccccga ggaccgggcg
     3781 ctctctatcg tgacacgcgg gagtttgcgt tgcctgctaa gcgaggcaac gggcattgcg
     3841 ccggacaaaa tcctcttggc agaaggacct ttcggaaagc cctatctttg cggactccgc
     3901 gggccacatt tcaacgtgtc ccattcgggc tcttttgcat tgattggatt atctgacagc
     3961 cgcccgatcg gcgtcgacat cgagcggatg cgtgtcacag gcgacgaact aaacgtggcc
     4021 cggagttatt tctccgccac cgaatatcgc gctcttcaag gcctgtggaa ggacgcacgc
     4081 ctttcttcct tctttaaaat atggacctgc aaggaagccg ttctcaaagc ctgtggcgcc
     4141 ggaatatccg aacatcttaa agatttttcc gtagaattga ccacggatgg ttttgcggtc
     4201 catcctgagc ctcattggtt tgcgcctgca cttgcctcgg tttccgccaa gccggtggag
     4261 gtcccgccgg gttatgttgg atgctgcgcg ctagcctgaa ttgtgcgtaa aaggaagacc
     4321 ggctggacga ttcctatttt tgccttaatt tcttttccta attttgccta ttaatttttc
     4381 gaggcaatgg tgaggctcgg tctgagctgg atctcactca gcggcctcta c
//
LOCUS       DATMPU010000080         4391 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_2853327,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000080 DATMPU010000000
VERSION     DATMPU010000080.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 4391)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4391)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4391
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2853327"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..305
                     /locus_tag="VNY06_01870"
     CDS             <1..305
                     /locus_tag="VNY06_01870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX13620.1"
                     /translation="GHAILLSEARLLTLDLQTGRARPVTALPTMIPTSRREVCYRASG
                     LVLWRKCEVRPYPLYGPYWRESRLSASHLHPPSVSRTPAARVLRDARTRGTSRVNL"
     gene            complement(466..2610)
                     /gene="pnp"
                     /locus_tag="VNY06_01875"
     CDS             complement(466..2610)
                     /gene="pnp"
                     /locus_tag="VNY06_01875"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012589317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HWX13621.1"
                     /translation="MFEIHREELDWAGRKLVLETGRVARQADGAVVATYGETTVLATV
                     VSAKSAKAGTDFFPLTVNYQERAFAAGRIPGGYFKREGRATEKETLVSRLIDRPIRPL
                     FPNGYRNETQVIATVLSHDLENDPDVVAMVASSAALTLSGIPFMGPVGATRVGFINGT
                     LNINPSLEALKNSDLDLVVAGTGDAVLMVESEAKELSEAVMLEAVMSGHKAFQPVIEA
                     IIRLAEKAAKPPRDLAAADKSAVEAAVRELAEAELREAYKITAKQERYGAVDAVKEKV
                     TAVLLPANGNTKFSAEEVAKVFYELQAKVVRWNILDTGIRIDGRDVRTVRPILAEVGV
                     LPRTHGSALFTRGETQAFVVATLGTGEDEQFIDSLEGTYKERFLLHYNFPPFSVGETG
                     RLGSPGRREIGHGKLAWRAIHPMLPSAAEFPYTIRVVSEITESNGSSSMATVCGSSLA
                     LMDAGVPLKRPTAGIAMGLILEGGRYAVLSDILGDEDHLGDMDFKVAGTEEGVTSLQM
                     DIKITGINEEIMRLALEQARDGRLHILGEMAKALSGARAELGEFAPRIESFRIPTEKI
                     REVIGTGGKVIREIVEKTGAKVNIEDDGTVKVASSDGDSIRAAINWIKSIASDPEVGQ
                     IYDGTVVKVVDFGAFVNFFGSKDGLVHISQLSKGRVAKTGDVVKEGDKVKVKLLGFDD
                     RGKVRLSMRLIDQTTGEDLEGKERLEQQEAGE"
     gene            complement(2929..3198)
                     /gene="rpsO"
                     /locus_tag="VNY06_01880"
     CDS             complement(2929..3198)
                     /gene="rpsO"
                     /locus_tag="VNY06_01880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011995947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HWX13622.1"
                     /translation="MSITAERKQALINDYAQKPGDTGSPEVQVAILTERIANLTEHFK
                     THLKDNHSRRGLLKLVSQRRQLLDYVKTRDEPRYKSLIERLGIRR"
     gene            complement(3847..4056)
                     /locus_tag="VNY06_01885"
     CDS             complement(3847..4056)
                     /locus_tag="VNY06_01885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX13623.1"
                     /translation="MLDSCLGLRDGEEIQKKGIAVFHKFFGSNALFLWKSVVGDRGDG
                     LHVPFLTGLGDEVVWPGTGSTTPGP"
BASE COUNT          929 a         1266 c         1213 g          983 t
ORIGIN      
        1 tcggtcatgc gatactccta tctgaagcga ggttgctaac cctcgatctt cagacaggac
       61 gcgcccgccc cgttaccgcg ctgcccacga tgatacccac cagccggcgc gaggtgtgct
      121 accgcgcgag cggtttagtt ctgtggcgca aatgcgaagt gcggccgtat ccgctatatg
      181 gtccctattg gcgggaaagc agactctcag cgagccacct tcatcccccc tcggtgagcc
      241 gcactcccgc agcccgagtc ctgcgcgatg cgcgaacacg tgggacgtcg cgcgtcaatc
      301 tctgaccaga ggcagggcct gacggcgcag gagtcaataa atttccggcg ccctgctttg
      361 aattcgacct tcatgttcgt catgccaggt ccccaccgcc tgagcgcgcc gatctcaatg
      421 ccgctggtat cagagcggcc ttttatgcgg gtggggattt aagcgctatt ccccggcttc
      481 ctgctgctcg agcctttcct tgccctcaag atcttcgccg gtcgtttgat cgatgaggcg
      541 catcgaaagg cgcaccttgc cgcgatcatc aaagcccagg agcttgactt tgaccttgtc
      601 gccttctttc accacatcgc cggtcttggc gactcggccc ttggacagct gcgaaatatg
      661 gacgagcccg tccttcgagc caaagaagtt tacgaaggcg ccaaaatcaa ccactttcac
      721 gaccgtgccg tcatagatct ggccgacctc cggatcgctt gcgatggatt tgatccaatt
      781 gatcgcggcg cgaatcgaat cgccgtcgga agaagcaact tttacggttc catcatcctc
      841 aatattgacc ttggcgccgg ttttctcgac gatctcgcgg attactttgc caccggtacc
      901 aatgacttcg cggatttttt cggtgggaat tcgaaacgac tcgatgcggg gtgcgaattc
      961 gccaagttcc gcccgcgcgc ccgaaagcgc cttggccatt tcgcctaaga tatgcagccg
     1021 gccgtccctt gcttgctcca aggcgaggcg catgatttct tcattgatgc cggtgatttt
     1081 tatatccatt tgcagcgatg tgacgccttc ctcagttccg gcaaccttaa aatccatgtc
     1141 gccgagatga tcctcgtcgc cgagaatatc ggagagtacg gcgtagcgtc cgccttcaag
     1201 aatcagaccc attgcgatgc ctgcggttgg ccgtttcaac ggcacaccgg cgtccatcaa
     1261 tgcaagggac gagccgcaaa cggtcgccat cgaagaggag ccattcgact cggtgatctc
     1321 ggagaccacg cggatcgtgt agggaaactc cgccgcgctt ggcagcatcg gatgaatggc
     1381 gcgccaggcg agcttgccat ggccgatttc gcgccgtccc ggcgatccga gccgccccgt
     1441 ttcgccgacc gagaagggcg ggaaattata atgcagcagg aagcgctcct tataagtccc
     1501 ctccaaactg tcgatgaact gttcgtcctc tcccgtaccg agcgttgcga caacaaacgc
     1561 ctgtgtctca ccccgggtga aaagcgccga cccatgggtg cgcggcagga cgccgacctc
     1621 cgcgaggatg gggcgcacag ttcgcacatc gcgcccgtca atgcggatac ccgtgtcaag
     1681 gatgttccac cgcacgacct tggcttgcaa ttcgtaaaag accttggcga cctcctccgc
     1741 ggagaatttt gtattgccat tggctgggag aagcaccgcc gtgacttttt ctttgaccgc
     1801 gtcgaccgcg ccatagcgtt cctgtttggc ggtgatctta taggcctcgc gcaattcggc
     1861 ttcggccaat tcgcggaccg ctgcttcgac cgcggacttg tcagctgcgg cgagatcgcg
     1921 cggcggcttg gcggcctttt cagcaagacg gatgatcgct tcgatcaccg gctggaaagc
     1981 cttatggccg ctcatgacgg cctcgagcat cacagcctcg gaaagctcct tggcttccga
     2041 ctcaaccatc agaacggcat cgcccgttcc agcgacgacg agatcaagat ccgagttctt
     2101 cagcgcttcg agtgacggat tgatattcag cgtcccgttg atgaaaccga cacgcgtggc
     2161 tcccaccggc cccatgaagg ggatcccgga taaagtgagt gccgccgagc ttgcgaccat
     2221 ggccacgaca tcggggtcgt tctcgaggtc gtgcgaaagg actgtcgcga tgacttgtgt
     2281 ctcgttgcga tagccgtttg gaaacaaagg gcggattggg cggtcgatga ggcgcgagac
     2341 gagggtctcc ttttcggtcg cccggccttc gcgcttgaag tagccgccgg gtatgcgccc
     2401 agccgcaaag gctctttctt gataattgac ggttagcggg aaaaaatcgg ttccggcttt
     2461 ggctgatttc gccgatacga ctgtggcaag gacagtggtt tcaccataag tcgcaacgac
     2521 ggcaccgtct gcttggcggg caactcttcc cgtctcgaga acgagcttgc gcccggccca
     2581 gtcgagttct tcacgatgta tttcgaacat tcacgcgtct ttcttgggta gcgatgaaac
     2641 ccgcggccaa agcaagatgg cgagaagctg ttccacagcg tcttgcgatc ctgccatgag
     2701 cgatgagatt caccatgctt tggcgtggcg gccccatgcc gccacattcc tttccttgcg
     2761 ccagaaagtc cttagaactc tgccgctttt cgccaatcgt tcgcgtgcga gctctttaaa
     2821 cttgcttggt tctgacgatg aacgtcagaa cctgtatttg accttggagc attccctaca
     2881 agcgccctta tcgaggcacg acgcggaaac tgcatccgag ggacctccct agcgccttat
     2941 gccgagccgt tcgatgagag acttataccg cggctcatct cttgtcttga catagtcgag
     3001 cagctggcgg cgctgggaaa cgagcttgag gagcccacgg cgcgagtgat tgtctttgag
     3061 atgggtctta aaatgctcgg tcagatttgc gatacgctca gtcaaaatcg ctacctgaac
     3121 ctctggagaa ccggtgtcgc ccggcttttg ggcatagtca ttgatcagcg cctgtttgcg
     3181 ctcggccgta atcgacatca gaagacctcg ttggaaaaat gatgcgagac aagcctcggc
     3241 aagtcgaagg caagccccca attgcgccag cgaaaaggtt ttttcacggc tgctgcacta
     3301 gggaaaattt tccggaccac gcggcccggc cgggatgtcg tccagcaggg tcgagcgaat
     3361 ggcgcattct cgagcaggtc gtgtgtttat acatgaaacc agccccaggg gaaggggtag
     3421 ggctgatgaa acgcctttat ttgaagttgt ttttccacaa atgcaattcc gggcagcaag
     3481 aggctattca gcgcgtttct tttatcgatt cttggggttt cacagataaa ttcaatgtat
     3541 tccagcggtg atggtcggat ggagttataa atgctcgaga atttcgtttc cttgggaaca
     3601 attttacatg ttcttacggg taaactaaac cacgaagtca tcgcattcta cgtgtttgcc
     3661 gctgctacta tattactatt ttgtctcgtc attttaattt gtagactgac gcgacggacc
     3721 aagaaaaagc aagttctgcc tagccctgcg aacaatttgc cagcccttac ggacaaaaga
     3781 aacgacggca aggcccagga taaacaggct gattcgccgt caagctctcg gtcgccggaa
     3841 gcctcgctaa ggcccaggcg tggtcgagcc agtaccaggc cataccactt cgtcgccgag
     3901 cccggtgagg aacgggacat ggaggccgtc gccgcggtcc ccgacgaccg atttccacag
     3961 gaacaacgca ttgctgccga agaacttatg gaatactgcg atgcccttct tctggatttc
     4021 ctcgccgtcg cgcagaccga ggcaactgtc gagcatgttg tttcttagta agtcattttg
     4081 gcgccacgag aggggcaccg atgggccgat gttagacact ggggtcgaaa aaacagcttc
     4141 gaacgtggga atgagctgcc tgaagtgctg agacaggcgt aagggcatcc tcgttcgcga
     4201 ggaatgccaa caacttgacc gatcccacag agggcagcat ttaccaaacg ctaaccccat
     4261 acttcaaaat attcaaaatg atgagcctat cgctgccgac atcccgagtg ctgaaagctc
     4321 gcaacagcgg ttttattagg gatttgaaac ggaaacaact aaagggcctc gctccttgca
     4381 gagattcacg t
//
LOCUS       DATMPU010000081         2905 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_62508, whole
            genome shotgun sequence.
ACCESSION   DATMPU010000081 DATMPU010000000
VERSION     DATMPU010000081.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 2905)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2905)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2905
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_62508"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..323)
                     /locus_tag="VNY06_01890"
     CDS             complement(<1..323)
                     /locus_tag="VNY06_01890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008143951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX13624.1"
                     /translation="MRQNCTQAAFRVDRIGSHYFTVGGRSSIRRLAYQRRAARTGFLR
                     PGIVWLGGIKSHMNGVKASLLDRYAAKTGRAFLRFDYSGHGRLMAFLKPARLASAESA
                     KETGRS"
     gene            495..1616
                     /locus_tag="VNY06_01895"
     CDS             495..1616
                     /locus_tag="VNY06_01895"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020174813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HWX13625.1"
                     /translation="MPSSRAAIGVAAALSAVGARALVACTDRLKGELQAKGGIFLPFP
                     LRSKNPLTMALRVRRLARLIEAERADIVHVSSRALGWIAYGATRLTKTPLVTSFGSSY
                     EGCNPIAVRYNSVLARGDMVLVESNFAADLVAKHYPSAAGRIRVIHRGIDCQVFAPSA
                     VAPARVEEVRRQWKVAPHEQIVLVSGPTRFGSGHKILVEAARLLSRSGLSGVKFILSS
                     DNREPVACGRDIDRAIAQEGLQGIMYRAGHCDMSAALLAATIVVMPATGPRDLGEAAV
                     NAQAMGTPVIAANLGAAPEIILAPPAVAESSRTGFLVPPGDAPALAVTIASVLTLGAT
                     AGSRLSSRAIEHVEARYSAARMCAETLKAYVDVRRSGDL"
     gene            1789..2316
                     /gene="infC"
                     /locus_tag="VNY06_01900"
     CDS             1789..2316
                     /gene="infC"
                     /locus_tag="VNY06_01900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018267530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HWX13626.1"
                     /translation="MKAAPVPQKDGPRINHEIRVREVQLIDAEGQNRGVVDIYEAQQT
                     AEEAGLDLVEIVPSAIPPVCKILDYGKFRFLEQKKSAEARKKQKIVEVKEIKLRPGID
                     DHDYGVKMKAVRRFFEEGDKVKVTLRFRGREMAHQDLGLRLLERVKSEIVNIAKVEIE
                     PSIEGRQMVMVLAPK"
     gene            complement(2407..>2905)
                     /locus_tag="VNY06_01905"
     CDS             complement(2407..>2905)
                     /locus_tag="VNY06_01905"
                     /inference="COORDINATES: protein motif:HMM:NF014715.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005536 - glucose binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0051156 - glucose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HWX13627.1"
                     /translation="LGTEEAVIWPYLDKGSLGRVCAETILSGHGLLRLHRARCAARGV
                     ATAIPSEIALIDKARENHRGEEAATLRHCWLLVARFTGDLALAFLAKGGVTLAGGILP
                     RILEFCDPARFRAAFESKTPYGELMRGIGTRLIVAENTVFSGMAEIAAAPQDFAINYA
                     QRAWR"
BASE COUNT          603 a          847 c          860 g          595 t
ORIGIN      
        1 gaccgtccgg tttccttggc ggattctgcc gaggccaatc gtgccggctt caaaaacgcc
       61 atcagacggc catgaccaga atagtcgaaa cgcaaaaaag cgcgtccggt cttcgccgcg
      121 taacgatcga ggagcgatgc cttgactccg ttcatatgcg atttgattcc gccaagccaa
      181 acaatgcccg gccgaagaaa ccctgttctt gccgcgcggc gctgataggc taatcggcgg
      241 attgatgaac ggcctcccac ggtgaagtaa tgggaaccga tccggtccac tcgaaatgcg
      301 gcttgcgtgc aattctgcct catcgctttg ctcgcttcct gatacctaaa aattaaccag
      361 tttagtcttg gctttgggct tcctgagcgc caactcgtcc aatgccgtca attacgagtt
      421 tacctttcca cgacagcgtc cggcacccgc tggataaggt aacggtctta caaattgttc
      481 ctgggaatga ggtcatgcct tcatcccggg ccgcaatcgg cgtcgccgca gcgctcagtg
      541 ctgttggtgc gagagcgctc gttgcatgca cagatcggct gaagggtgag ttgcaggcca
      601 agggtggaat atttttgccg ttccccttgc gcagcaaaaa tccgctaacc atggccttga
      661 gggtccgacg tctcgctcgc ctcatcgaag cagaacgcgc cgatatcgtg catgtgagct
      721 cccgagcgtt gggctggatc gcttatggcg ccacgaggct gacgaaaacc ccgttggtga
      781 cgagttttgg ttctagctac gaaggctgca acccgattgc ggtgcgctat aattcggtgc
      841 tggcgcgggg cgatatggtt ctcgtcgagt caaacttcgc cgctgactta gttgcgaagc
      901 actatccgtc ggcagcagga agaatccgcg tcatccatcg ggggattgac tgtcaggttt
      961 ttgcaccgag tgccgtcgcg cctgcccggg tcgaagaagt tcggcggcag tggaaagtgg
     1021 cgccgcatga gcagattgtg cttgtatcgg gaccaacgcg cttcggcagt ggccacaaga
     1081 tcctggttga ggcagcgcgg ctcttgtcac gaagcggcct ctcgggggta aaattcatcc
     1141 tctccagcga caaccgggaa ccggtcgcgt gcggccgcga catcgaccgc gcgatcgcgc
     1201 aggagggact gcaaggcatt atgtaccgtg ccggtcactg cgacatgtca gcagcgcttc
     1261 tcgccgctac gatcgttgtc atgcccgcga ccggcccgcg agacttgggc gaggccgccg
     1321 tcaatgccca ggcgatggga acaccagtca tcgcagccaa tttgggggct gcgccagaaa
     1381 tcatcctcgc gccgccggcg gtggccgaat cctcacgcac gggatttctg gtgccgccag
     1441 gcgacgcgcc agcgttggcc gtaacgattg ccagcgtgct aactctcggt gccaccgcgg
     1501 gcagcaggct ctcatcgcgt gctatcgagc atgtcgaagc acgctattcg gctgcgcgta
     1561 tgtgcgccga aaccctcaaa gcctatgttg atgtgcggcg cagcggggat ttgtgaccag
     1621 cggcccacat ttttcgcctc ccgctgggtg acctcgtcat tgacagaacg gtccaatcaa
     1681 ccgatgtttc atccgggtcc ggccatcgtc acgagcctcg ttcgtgattc gcgcgtagag
     1741 ccgggttcga ttcatcggca aaaaggagat ttagccattc gccgccctat gaaagccgcg
     1801 cctgtcccac agaaggatgg accgcgtatt aaccatgaaa tccgcgtccg cgaagttcaa
     1861 ttaatcgatg ccgaaggcca aaatcgcggt gtcgtcgaca tttacgaagc tcagcaaacg
     1921 gccgaggaag cgggacttga tctcgttgaa atcgttccat cggctattcc cccagtctgc
     1981 aagattttgg attacggtaa gtttcggttt ctggagcaga aaaaatccgc ggaagcccga
     2041 aagaagcaga agatcgttga ggtcaaggaa atcaagctgc gcccgggaat tgacgaccat
     2101 gattacggcg tcaagatgaa agccgttcgc cgctttttcg aggagggtga caaggtcaaa
     2161 gtcacgttgc gcttccgggg ccgcgaaatg gcgcatcagg atttgggcct gcggcttctc
     2221 gaacgggtaa aatccgagat cgtgaatatc gccaaggtcg aaatcgagcc atcgatcgaa
     2281 ggccgccaaa tggtcatggt gctcgcgcct aaatagagca gcgagcgtgc ccaatcccgt
     2341 caagcgataa atcggcggaa gttggctgag aggttgcggc tgacgcgacg gtgatctcat
     2401 gggcgcctat cgccatgcgc gttgcgcgta attgatcgca aagtcttgcg gcgctgcagc
     2461 aatctcggcc atcccggaga agaccgtgtt ctcggcgaca atcagccgtg tgccaatgcc
     2521 gcgcatcaat tcgccatagg gcgttttgct ctcaaacgct gcacgaaaac gcgccggatc
     2581 gcaaaattcc aatatccttg gcaagattcc tcctgccaag gtgacgcccc ctttcgccag
     2641 aaacgcgagc gcgaggtcgc ccgtaaaacg tgcgacgaga agccagcaat gccgcagcgt
     2701 cgctgcttcc tcgccgcggt gattttcgcg ggccttgtca atgagcgcta tctcgctggg
     2761 gatggcggtg gcaacgcccc gcgccgcgca tcgcgcgcgg tggagccgca ggaggccgtg
     2821 gcccgataga atcgtttcag cgcacactct gccaagcgac cctttgtcca ggtaaggcca
     2881 gatcaccgct tcttcggtcc cgagc
//
LOCUS       DATMPU010000082         3423 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_3781297,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000082 DATMPU010000000
VERSION     DATMPU010000082.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 3423)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3423)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3423
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3781297"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2273)
                     /gene="valS"
                     /locus_tag="VNY06_01910"
     CDS             complement(<1..2273)
                     /gene="valS"
                     /locus_tag="VNY06_01910"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011158133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HWX13628.1"
                     /translation="MMEKTFDPKSVEGRIAAAWREAGAFRADRPERAEAAPFTIVIPP
                     PNVTGSLHMGHALNTTLQDILCRFERMRGKDVLWQPGTDHAGIATQMVVERQLMENQE
                     PGRHVLGREKFLERVWQWKAQSGGAIIEQLQRLGASCDWSRERFTLDEGLSRAVTKVF
                     VELYKQRLIYKDKRLVNWDPELQTAISDLEVKQVEKRGSFHWSEDSGERLDIEALQKA
                     LNRDPNGHLYYLKYPILGRNDEFITVATTRPETMLGDMAVAVHPEDARYQNLIGKTII
                     LPLVDRPIPIIADEHSDPEKGTGAVKITPAHDFNDFEVWQRNAAAFRGFEEGFEYLPF
                     NIDEPINILTPSGQIKTSASDDFERKRGPIYPPVPDYLAKLGADANDEFGIPSDIPFE
                     LRGRDRFDARSKIVEMLAKKGLIKKIEPHVHMVPHGDRSGVVIEPRLTEQWYVKADVL
                     ALPALEAVRSGRAKFIPENWEKTYFQWLENIEPWCISRQLWWGHRIPAWYGPDGQVFV
                     APTLEVAKREAALYYASKGSFNTDYTSIDGLQLAADIRSGAYDLERGHDEDVLDTWFS
                     SALWPFSTLGWPDETPDLKRRYPTNVLVTGFDIIFFWVARMMMMGLHFMEEVPFRDVY
                     IHALVRDEKGAKMSKSKGNVVDPLHLIDTFGADALRFTLAAMAAQGRDIKLSTQRLEG
                     YRNFGTKLWNAARFAEMNACVLEAGFDPKQTALALNGWIIGETAKAITEVTAAIETHR
                     FNDAANAAYKFVWNIFC"
     gene            complement(2667..>3423)
                     /locus_tag="VNY06_01915"
     CDS             complement(2667..>3423)
                     /locus_tag="VNY06_01915"
                     /inference="COORDINATES: protein motif:HMM:NF025847.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4436 family protein"
                     /protein_id="HWX13629.1"
                     /translation="LEFAEPGSTTPLNIYVTMIDMDPVREELTVLLDFATEIGPHGKH
                     FPGVPRRDLTVHVGDGSFVQDIALKAQLRAPVRSLFASLYADLSAVILSISMRAASRS
                     ARLKRQGRRPQPVRLTVWPAMLQWTVGIEQTDPAATSNPGVDLAIRITRPTPFVVIAV
                     AVYTAMAVVGLSGLMIGALVFLGVRRVEATLTGTLSAMIFAVPALRSSLPGVPPLGGH
                     ADIVVFVWVELAVIIGLILFMIAWMERGLSPES"
BASE COUNT          783 a          970 c          921 g          749 t
ORIGIN      
        1 tcgcaaaaaa tgttccagac aaatttatag gcagcgttgg cggcatcatt gaaacgatgg
       61 gtttctattg ccgctgtcac ctcggtaatt gccttcgccg tctcaccgat aatccagcca
      121 ttgagcgcaa gagccgtttg cttaggatcg aagccagcct ctagcacgca ggcgttcatt
      181 tcagcaaaac gcgctgcgtt ccagagcttt gtgccaaaat tccgatagcc ttcgaggcgc
      241 tgcgtggaga gttttatatc gcgcccctgc gccgccatcg ccgcgagcgt gaagcgcaag
      301 gcgtccgccc caaaagtgtc gatgaggtgc aagggatcga cgacattgcc cttggatttc
      361 gacatcttcg cgcccttctc gtcgcgcaca agggcgtgga tatagacatc gcgaaagggg
      421 acttcctcca tgaaatgaag gcccatcatc atcatccgtg caacccagaa aaagatgatg
      481 tcgaagccgg tgacgaggac attcgtcgga taacggcgtt ttaggtcagg cgtctcgtcc
      541 ggccagccaa gggtggaaaa tggccaaagc gcggaagaaa accaagtgtc gaggacgtct
      601 tcgtcatgac cacgttcaag atcataggcg ccacttcgaa tgtcggcggc cagttgtagt
      661 ccgtctatcg aggtgtaatc agtattgaag ctgcctttgc tcgcgtagta aagcgcagcc
      721 tctcttttgg caacttcaag agtgggtgcg acgaaaactt gcccatccgg gccataccac
      781 gcaggaatcc gatggcccca ccagagctga cgcgaaatac accaaggctc aatgttttcc
      841 aaccattgga agtaggtctt ttcccaattc tcgggaatga atttcgctct gccggagcgc
      901 acagcctcca gagccggaag agccagaacg tcggctttca cataccattg ttcggtaagg
      961 cgcggctcaa tcaccacacc agatcgatcc ccatgaggaa ccatatgaac atgcggttcg
     1021 atttttttga taagtccttt ctttgccagc atttctacga ttttggagcg agcatcgaaa
     1081 cgatctcgtc ctcgaagttc gaacggaata tcagagggaa taccgaattc atcattggcg
     1141 tccgcgccca acttcgcaag gtagtcgggc acaggtgggt atatcgggcc tctcttgcgt
     1201 tcaaagtcat ctgacgccga tgttttgatt tggccgctcg gcgtcaaaat gttgataggc
     1261 tcgtcaatat tgaatggcaa atattcaaag ccttcttcaa aaccccggaa ggctgcggcg
     1321 ttgcgttgcc aaacttcaaa atcattgaag tcgtgcgccg gggtgatctt aaccgcgcca
     1381 gtgccttttt caggatcgga atgctcatcc gcgataatcg ggatcggccg atccacgagc
     1441 ggcaagataa tggtttttcc aatgagattt tgataacgcg cgtcctccgg atgtacggcg
     1501 acggccatat ccccgagcat ggtttccggg cgggtcgtcg caacggttat gaattcgtca
     1561 ttccggccaa gaatcggata cttaagataa tagaggtgcc cgttcgggtc gcggttcaat
     1621 gccttttgaa gtgcttcaat atctagccgc tcgccgctat cctccgacca gtgaaagctg
     1681 ccgcgtttct cgacctgttt gacttcgagg tcggagatcg cggtttgaag ttctgggtcc
     1741 caattgacga ggcgcttatc cttatagatc aggcgttgct tatagagctc aacgaaaacc
     1801 ttcgtaacgg cacgcgaaag tccttcatcc agggtaaagc gctcacgcga ccagtcgcag
     1861 gaagcgccga gcctttgcaa ttgctcgatg atcgccccgc ccgactgcgc tttccattgc
     1921 catacacgtt cgagaaattt ctctctgcca aggacatgcc ggcccggctc ttgattctcc
     1981 atcaattgcc gctcgaccac catttgcgtg gcgatgcctg cgtgatcggt ccccggctgc
     2041 caaagtacgt ccttgccgcg catgcgctca aaccggcaaa gaatatcctg taacgttgtg
     2101 ttgagcgcgt ggcccatatg cagggagccg gtcacattgg gcggcggaat gacgatcgtg
     2161 aaaggcgcgg cctccgcacg ctccgggcga tcggcgcgaa atgcgcctgc ctcccgccat
     2221 gccgccgcta tgcggccttc gaccgatttc ggatcgaaag tcttttccat catctcatca
     2281 ttgttcggca aaaccccgcc gccaggcgag gcctttcaaa agtgagcgat ggcaaacaag
     2341 cggacaaaat gcaagttagc ggcgaaccaa gcactcgcgg caccaacctt ttggtccgcg
     2401 aggcaagact aaaggcaaaa ttggccaacc aaaatcttcc gctatcttgc tatctctgct
     2461 caagtgcttt tagggcgttt aatggatccc tgacgagatc ttgaagtcca atcgcaagcc
     2521 tggtccgtcg acgcgtctta caccgcagcg ctcgaataga gtccgagcaa gggtcaagcg
     2581 gtccaatagc caacgcgcgg cgtcgtccaa atgttttata ttgcaatttg cgcgattatt
     2641 gccggctttt ctcagctata attcgatcaa gattccggag aaaggccgcg ctccatccag
     2701 gcaatcatga aaagaatgag gccaatgatc acagccagct ctacccacac gaacacgacg
     2761 atatccgcgt ggccacccag cggcggtacg ccgggcagcg aactgcgcag ggccggcacg
     2821 gcgaagatca tggcacttag cgtgccggtc agcgtcgcct cgacccgccg aacgccaagg
     2881 aaaaccagag cgccgatcat cagcccgctc agcccaacca ctgccatggc tgtgtagaca
     2941 gctacggcaa tcacgacgaa gggcgtcggg cgcgtgatgc ggattgctag atcaactccc
     3001 gggttgctcg tcgcagcggg atcggtttgc tctatgccaa ctgtccattg caacattgca
     3061 ggccagaccg tcaggcgaac tggctggggg cgtctccctt gccttttcaa acgcgctgac
     3121 cgagatgcgg ccctcataga gatcgaaagg ataaccgctg acaaatccgc gtaaagagat
     3181 gcgaagagtg atcggactgg cgctctgagt tgcgccttca gcgcaatgtc ttgcacgaaa
     3241 ctgccgtcgc cgacgtgcac ggtcagatcg cggcgcggta ctccggggaa atgctttcca
     3301 tgcggcccaa tttcggtggc gaagtccaga aggaccgtca gttcctcgcg gactggatcc
     3361 atgtcgatca tcgtgacata gatgttgaga ggcgttgtcg atcccggctc ggcaaactcc
     3421 agc
//
LOCUS       DATMPU010000083         4671 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_3161655,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000083 DATMPU010000000
VERSION     DATMPU010000083.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 4671)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4671)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4671
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3161655"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..120
                     /locus_tag="VNY06_01920"
     CDS             <1..120
                     /locus_tag="VNY06_01920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HWX13630.1"
                     /translation="LIDEIAAWEDDRNVSHAKADWQFTTADARIKLKYLYPSI"
     gene            434..3409
                     /gene="uvrA"
                     /locus_tag="VNY06_01925"
     CDS             434..3409
                     /gene="uvrA"
                     /locus_tag="VNY06_01925"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020175278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HWX13631.1"
                     /translation="MTGAPDLLSTLKPSPERAQKNPPLSGPPEAPLKGNVIKIRGARE
                     HNLKNIDLTIPRGKLVVFTGLSGSGKSSLAFDTIYAEGQRRYVESLSAYARQFLEMTQ
                     KPDVDQIDGLSPAISIEQKTTSKNPRSTVGTVTEIYDYMRLLFARIGIAYSPTTGLPI
                     ESQTVSQMTDRVMALPEGTRLYLLAPVIRGRKGEYRKEIAEFTKRGFQRLKIDGAFYE
                     ITDAPTLDKKLKHDIDVVVDRIAVRPGIEARLSESFETALELADGIAIAEYADEKDDS
                     SSPKRIVFSSKFACPESGFTIPEIEPRLFSFNNPFGACPHCGGLGAEQTADPELIVPD
                     PKLTLRKGAIAPWARSSSPYYLQTLEALAKHYKFRLDTRFESLPEKVRDVILYGSGAE
                     EVRFSYDDGMRGYDVVKPFEGVAANLERRFHETESEWARDDIARYMTASPCGVCNGAR
                     LRPEALAVKIEGYDISQLTGLSVHDARAWFAELPGKLDEKRGDIARRILKEISDRLSF
                     LADVGLGYLTLARSSGTLSGGESQRIRLASQIGSGLTGVLYVLDEPSIGLHQRDNGRL
                     LETLRRLRDLGNTVIVVEHDEDAILTADHVVDVGPGAGIHGGEIVAQGTADELMENPN
                     SLTGQYLRGTLDVAMPRHRRAPRRGHKLTIVNAHGNNLKNISAEIPLGLFVCVTGVSG
                     GGKSTLIIDTLYKAAARRLNGAFEHPSPHSSIEGLEYLDKVIDIDQSPIGRTPRSNPA
                     TYTGAFTPIREWFAGLSEAKARGYQPGRFSFNVKGGRCEACQGDGVIKIEMHFLPDVY
                     VTCDVCKGKRYDRETLEVKYRGHSIADVLDMTVEETATLFRAVPSIREKMATLARVGL
                     GYIKTGQQANTLSGGEAQRVKLAKELSKRATGRTLYILDEPTTGLHFHDVAKLLNVLH
                     ELADQGNTVVVIEHNFEVIKTADWIIDLGPEGGDGGGEIVAAGPPEVIMKAKNSHTGR
                     YLREFLQRRPLAKDKQAAE"
     gene            3584..4174
                     /locus_tag="VNY06_01930"
     CDS             3584..4174
                     /locus_tag="VNY06_01930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX13632.1"
                     /translation="MRAVVQHCLPDEHFDCMCLNGQDFTSLWVRPTATIAKMFHQLPA
                     EKQRAVRCAIGEHVTMDVAAIFDRPSKFFTIVREPVDRVISNFFFNRTATDLTSYPFI
                     KDLTLEEYLDSGLGLDHNNHQVRMLSNCPELDAPWDPKGRPISTPPVERRHLEMAKRN
                     VEKRFIVAAPMEKYTALVWFLKRLYGWPPIGCFSGS"
BASE COUNT         1065 a         1373 c         1259 g          974 t
ORIGIN      
        1 ctcatcgacg aaatcgcagc ttgggaagat gatcgaaatg tcagccacgc caaggccgac
       61 tggcaattca ccacagcgga cgcacgtatc aaactcaagt acctataccc gtcaatctga
      121 ctgaatcgag ccactagaca gtttttcggc ccgatccgac atgcttcaca gcgttcgctg
      181 tttctggctt gtaagaattt tgcccgcgac cggacaaaaa tgtttcacgt gaaacatttt
      241 ggtacgattt ttcacacgtc gcgcacgtaa aacttatccc ggttttcttg aacccggctg
      301 cgttgcaaaa gcaaaccggc ccgctacatt gaggcacagc cttgcatccg gcggcccaat
      361 ttcttaacgg cgcagatcgc ccattcagat tgcctcgatg aagcaaccga aaccgcacgc
      421 gacctccggc caaatgaccg gcgcgccgga ccttctttca acattgaagc cttcgccgga
      481 aagggcgcag aaaaacccgc ccttgagcgg acctccggaa gcgccgctca aaggcaacgt
      541 gataaaaatt cgcggcgccc gcgagcacaa tctcaaaaac attgacctca ccatccctcg
      601 cgggaaactt gtcgtgttta cgggcctttc cggctcggga aaatcttcgc tcgctttcga
      661 taccatctat gcggaaggcc agcgccgcta tgtcgaatcg ctctcggcct atgcgcggca
      721 gtttttggag atgacgcaaa aaccggacgt cgatcagatc gacggcctct cccccgcaat
      781 ctcaatcgag cagaagacga cctccaaaaa tccgcgctcg acagtcggca ccgtcaccga
      841 gatctatgat tacatgcggc tgctcttcgc ccgcatcggc atagcctatt cgccaacgac
      901 ggggctgccg atcgaaagcc agacggtgag ccaaatgacc gaccgggtga tggcgctgcc
      961 tgagggcacc aggctctatt tgcttgcgcc ggttatccgc ggccgcaaag gcgagtatcg
     1021 caaggaaatc gccgaattca ccaagcgcgg ctttcagcgg ctcaagatcg acggcgcttt
     1081 ttatgagatc acggatgcgc cgacgctcga taaaaagctc aagcacgaca tcgatgtcgt
     1141 ggtcgataga atcgcggttc ggccgggaat cgaggcaagg ctttcggaaa gctttgagac
     1201 cgcgcttgaa cttgccgatg gcatcgcaat cgccgaatat gcagatgaga aagacgatag
     1261 cagctcgccc aaaaggatcg tattttcgtc aaaattcgcc tgccccgaat cgggcttcac
     1321 catccccgaa atcgagccgc gcttattctc tttcaacaat ccttttggag cctgcccgca
     1381 ttgcggcggc cttggcgccg agcagactgc cgatcctgag ctcattgttc ccgatccaaa
     1441 actcaccctg cgcaaaggcg cgattgcgcc ttgggcgcgg tcaagctcgc cctattatct
     1501 gcagacgctc gaagccctag caaaacatta caaatttcgt ctcgacaccc gttttgaatc
     1561 gcttccggaa aaagttcgcg atgtgatcct ttatgggtct ggcgcggaag aggtgcgttt
     1621 tagctatgac gacgggatgc ggggttatga tgttgtaaaa ccttttgagg gcgttgccgc
     1681 aaatctcgaa cggcggtttc atgagacgga gagcgaatgg gcacgcgacg acatcgcccg
     1741 ctatatgacc gcttcgccct gcggtgtctg taacggtgcg cggctgcgtc cggaggcgct
     1801 ggccgtcaag atcgaaggtt acgacatcag tcaactgacc ggactttccg ttcatgacgc
     1861 gcgagcgtgg ttcgccgagc tgcccggcaa actcgacgaa aagcgcggcg acatcgcgcg
     1921 ccgtattctc aaggagatct ccgaccggct gtctttcctt gccgatgtcg ggcttggcta
     1981 cctcacgctc gcgcgttcct cgggcacgct ttctggcggc gagagccagc gcatcaggct
     2041 cgcttcccaa atcgggtcgg gcctaaccgg cgtgctttat gtgctggacg agccgtcgat
     2101 tggcttgcac cagcgcgaca atgggcggct tctcgaaact ttgcggcggc tgcgcgatct
     2161 tggaaataca gtcattgttg tcgagcatga cgaagacgcg attttgaccg ccgaccatgt
     2221 tgtcgatgtc ggtcccggcg ccgggattca tggcggtgag atcgttgcgc aaggcacggc
     2281 cgacgaactt atggagaacc cgaattccct aaccggacaa tatctccgcg gcacgctcga
     2341 tgtcgcgatg ccgcgccacc ggcgcgcgcc tcggcgcggc cacaagctca cgatcgtcaa
     2401 cgctcatggc aataatctca aaaatatttc ggcggaaatc ccgctcgggc tttttgtctg
     2461 tgtcaccggt gtctcgggcg gcggaaaatc gaccctcatc atcgatacgc tttataaggc
     2521 cgctgcccga cggctaaacg gcgcattcga acatccttca ccgcactctt caatcgaagg
     2581 tctcgaatat cttgacaagg tcatcgacat cgaccaatcg cccatcggcc gcacgccgcg
     2641 ctccaaccct gcaacctaca ccggagcctt cacgcccatt cgggaatggt ttgcgggcct
     2701 ctccgaagcc aaggcgcgcg gctatcagcc aggccggttc tccttcaatg tcaagggcgg
     2761 acgctgcgag gcctgccagg gcgacggggt catcaaaata gaaatgcatt tcctgcccga
     2821 cgtctacgtc acttgcgacg tctgcaaggg caagcgctat gaccgcgaga cgctcgaagt
     2881 gaaatatcgc ggccactcaa tcgccgatgt gctcgacatg acagtcgagg aaacggcgac
     2941 tttgttcaga gccgtgccct cgatccggga aaaaatggca acactcgcac gcgtaggcct
     3001 tggctatatc aagaccggcc agcaggccaa caccttatcg ggcggcgaag cgcagagggt
     3061 gaagctcgcc aaggaactct ccaagcgcgc gacgggccgc accctctata ttctcgacga
     3121 gcccacgacc gggttgcatt tccacgatgt cgccaaactt ttaaacgtgc tgcacgagct
     3181 cgccgaccag ggcaatacgg tggttgtgat tgagcataat tttgaggtca taaagactgc
     3241 ggattggatc atcgatcttg gccccgaagg cggcgatgga ggcggagaga tcgtcgctgc
     3301 aggcccgccc gaagtgatca tgaaggcaaa aaacagccac acggggcgtt acttgcgcga
     3361 attcctccaa aggcggcctt tggccaagga caagcaggcg gcggagtaac ctttgacagc
     3421 cacaaaacgg ccataatgtt gaactgagtt cggttgtgaa tgaaagaatt gtccacttgg
     3481 aggcacgaga aatgccattc ggttctcgcg tcagcaacat tcctggactt gccccacccc
     3541 cagttatgat tttcttccat gttccgaaac gggcggacga acgatgcggg ccgttgtcca
     3601 gcactgcctt ccagatgagc acttcgattg catgtgtctg aatggtcagg atttcacgtc
     3661 tctttgggtg cgacccacgg ctacaattgc caagatgttc catcaactgc cagcagaaaa
     3721 gcagcgcgcg gttcgttgcg cgatcggcga gcacgttacc atggacgtcg ctgctatttt
     3781 cgatcggcca tcgaagtttt tcacaattgt gcgcgagcca gtggatcggg ttatttctaa
     3841 tttttttttc aatcggaccg cgaccgacct gacaagctac cccttcatca aggatctgac
     3901 gcttgaagaa tatttggata gcgggttggg tcttgatcac aacaaccacc aggtgcgcat
     3961 gttgagcaac tgtccggagc tggacgcccc ctgggatccc aagggccggc ccatttcaac
     4021 tcctccggtg gaacgccgtc atctcgaaat ggccaagcgc aacgttgaga aacgcttcat
     4081 cgtcgcggct ccgatggaaa agtacaccgc tctcgtttgg ttcttaaagc ggctgtatgg
     4141 ttggccaccc atcgggtgtt tttccggatc ctaaacgaaa cgcccaagcg tccaaggctg
     4201 gacgccgtat ctgaaacgac acggaaacgc ctggaaacat caaatcagta cgacatcgag
     4261 ctgtatgaat gggtgaagga acgtttcgcc aagcaaacta accctctcga gcctgacttt
     4321 tcgcgggaag ttcgtcggtt cgagatgcta aattgctcct tccgtctgat tctcagactc
     4381 accccccgac caattcacga gacgataaag cacctgctgt tgtcaccgca ggcaatgcag
     4441 agttgaagaa tctgggttgg aaaaatggtg gcgcatacgc acaaccattg cgattaagcg
     4501 taagctctaa gccggcgatg ggccgcggct ctacataccc gatgagcccc cgagcggatt
     4561 gcatttccac gacatcgcca aacttttaga cgtgctgcat gagctcaccg accagggcaa
     4621 aaacagccac acagggcgtt acttgcgcga attcctgcaa aggcggcctt t
//
LOCUS       DATMPU010000084         2842 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_1922467,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000084 DATMPU010000000
VERSION     DATMPU010000084.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 2842)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2842)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2842
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1922467"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            528..1403
                     /gene="dapF"
                     /locus_tag="VNY06_01935"
     CDS             528..1403
                     /gene="dapF"
                     /locus_tag="VNY06_01935"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012590865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HWX13633.1"
                     /translation="MSPLGNRLVSKLNGLGNEIVVLDVRGTAMTVSGAEARAIAGGGD
                     LAFDQLMVLHDPRRAGADAFLTIHNSDGSRAETCGNGTRCVAFILYRETKREKIVLDT
                     CATRLECLRKSESVFTINMGRPHLAWNEIPLRDPFYDTRRIELQIGPIDDPILHTPSA
                     VNMGNPHAIFWVEDIEAYDLGKIGPLLENHPIFPARANISLAHVLSPEHIALKVWERG
                     AGLTRACGSAACAALVAAARNGFTGRRAIVSLPGGDLDIAWLESNDHVAMTGPVEFEF
                     ETRLDPALFTDIAAQ"
     gene            1400..2662
                     /gene="mtaB"
                     /locus_tag="VNY06_01940"
     CDS             1400..2662
                     /gene="mtaB"
                     /locus_tag="VNY06_01940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012590864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
                     /protein_id="HWX13634.1"
                     /translation="MTSEQEQQSVEVLTFGCRLNLVESEAMRRAALACGRSNLVIINT
                     CTVTAEATRQARQSIRRIKRERPEADIVVTGCAVQINPKYFADMPEVSRVIGNEEKAI
                     PMRWAKNDSVRVDVSDVMAAKGAPSLLSEGIADHTRAFLAVQTGCDHRCSFCIIPYAR
                     GPSRSVPLNEVMRAASRLASAGFREIVLTGVDLTSYGSDLCAAPSLGSLVRAILDAAP
                     SLERLRLSSIDCVEADEDLIAAFAEEPRLMPHLHLSLQSGDDLILKRMRRRHSRTDAV
                     SFCAVLRRLRPEIVFGADLIAGFPTETGTMFENTLALVEECGLTHLHVFPFSPRPGTT
                     AAKMPPVAPEIIRARAKRLREAGEAALREHLDAQTGKFFRLLTERGGFGRTEDFTRVR
                     VGDVPPSRMINVVIAGNDGRVLETMAPA"
BASE COUNT          579 a          830 c          841 g          592 t
ORIGIN      
        1 ggcatcgctt ggcactcccg gcggtggctg ggagcctgaa ccgaagggcg aggacggagc
       61 gggttgcgtg tcaccgcccg gcgaggcggg cgccggcggg ctgtccccgg cggcaggaac
      121 ctctcccggt gattgagcga gggcacgccc gtgcgacgca gccaatgccg ccagaaacag
      181 gcccaatgcc agcgcaagcc aatgcaggaa tttgatgctt cccgtcctca tgtaccgctc
      241 caaacttgtg gcttagccca acgacttcgt tgtcgaggcc gcggaggcta actctacacc
      301 atcctgcagc aaaaaaagcg agcggtcagt acaatctcgc attcagtcaa gaatatgata
      361 ttcttgaaaa ttgtatagca cgtctcgcct gggagacact atggaatgct cctgcgcgcg
      421 tgtggccggc cattaaggcg ggctttgacc cgtagctaag cgcaaatgca aaatcacgcc
      481 gtcgtctgcc aatgatgtaa gagccaagtc agaaggaaca gtgcatcttg agtcctctgg
      541 gaaataggct cgtcagcaag ctcaatgggc tcggcaacga gattgtggtc ctcgatgttc
      601 gaggcacggc tatgaccgtc agcggagcgg aagcgcgcgc aatcgctggc ggcggcgacc
      661 ttgctttcga tcagctgatg gtgctgcatg atccgcgcag agccggggcc gatgctttcc
      721 ttacaattca taacagcgat ggctcgcgag ctgaaacctg cggcaatggg acccgttgcg
      781 tcgcctttat tctctatcgg gagacgaagc gcgaaaaaat cgttttggat acttgtgcca
      841 cgcgactcga atgcctgcga aagtcagaat cggtgttcac gatcaatatg ggccgcccgc
      901 accttgcctg gaatgaaatc ccgcttcgcg atccgtttta tgatacccgc aggattgaat
      961 tgcagatcgg cccgatcgat gaccctattt tgcatacgcc gtcggccgtg aacatgggca
     1021 atccacacgc aattttctgg gtcgaggaca tcgaggcgta tgatcttggc aagattggtc
     1081 ccttgctcga gaaccatcca atttttcctg cgcgcgccaa tatttcgctt gctcatgtcc
     1141 tttcacctga gcatatcgcg ctaaaagttt gggagcgtgg cgcaggccta acacgagctt
     1201 gcgggtcggc agcctgcgct gctctcgtgg cggcggcgcg caacggtttt acaggccggc
     1261 gcgcaatcgt gagcttgcct gggggcgatc ttgacatcgc ttggctcgag agcaacgatc
     1321 atgttgcaat gacgggtccc gtcgaatttg aattcgagac gcggctcgat ccggcccttt
     1381 tcacagatat tgccgcgcaa tgacgtccga acaggaacag caaagcgtcg aagttttaac
     1441 cttcggttgc cggctcaatc ttgtcgagtc cgaggctatg cgccgagcgg ccctagcttg
     1501 cgggcgcagc aatctcgtca tcatcaatac ttgcactgtg acggcggaag cgacaaggca
     1561 ggcgcgccag tccattcgcc gtatcaagcg cgagcgtccc gaggccgaca tcgtcgtcac
     1621 cggctgcgcg gtgcaaatca acccaaaata tttcgccgac atgccggaag tttctcgcgt
     1681 gatcggcaac gaggaaaaag ccattccgat gcgctgggca aagaatgata gcgtccgggt
     1741 ggatgtttcc gatgtcatgg cagcgaaggg agccccctcc ctcttgagcg aaggtatcgc
     1801 ggaccatacc cgcgcgtttc tcgccgtgca gaccggctgc gatcaccgct gtagcttttg
     1861 tattatcccc tatgcgcgcg gcccgtcgcg ctcggtgccg ctcaatgaag taatgcgcgc
     1921 ggcatcccgc ctggcaagcg caggttttcg cgaaatcgtt ttgacaggag tcgatctcac
     1981 ctcctatggc tcggaccttt gcgccgcccc ctcgctaggg agcctcgtca gggcaatcct
     2041 cgacgcggcg ccgagccttg agaggcttcg cctatcgtca atcgactgcg tcgaggccga
     2101 cgaagatctc attgccgcgt ttgcagagga gccgcggctc atgccgcatc tccatctgtc
     2161 gctccaatcg ggcgatgatc tgattctaaa gcgcatgagg cgccgccatt cccgcactga
     2221 cgccgtcagc ttttgtgcgg tattgcgccg gctgcggccg gagattgtgt ttggggccga
     2281 tctcatcgcg ggctttccga ccgagaccgg gacgatgttt gagaacaccc tcgcgcttgt
     2341 tgaggaatgt ggcctcactc acctccatgt gtttcctttt tcgccgcggc ccggaacaac
     2401 cgccgccaaa atgcctcccg tcgcgccgga gatcattagg gcaagggcca agcgccttcg
     2461 cgaggcaggc gaagcggcct tgcgcgaaca tcttgacgcg caaaccggca agttttttcg
     2521 cctccttacg gagcgcggcg ggttcgggcg caccgaggat tttacaaggg ttagggtcgg
     2581 agacgttcct ccctcgcgaa tgatcaatgt cgttatcgcg ggcaatgacg ggagggtgct
     2641 cgaaacgatg gcaccggctt aaagcacgag aagatagttt cggcgatcca gaaacgggga
     2701 aaaaggagcc tcatgtcatc ggaccacctc gtcggtgcgg gaattcgcgc agtttgtccg
     2761 attctgggcc aaaaccttac caaggtccac ttcgaaggta ggttcacccc catagctacg
     2821 gactgcattc gaaggcaatc gg
//
LOCUS       DATMPU010000085         2035 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_372027,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000085 DATMPU010000000
VERSION     DATMPU010000085.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 2035)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2035)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2035
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_372027"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            67..786
                     /locus_tag="VNY06_01945"
     CDS             67..786
                     /locus_tag="VNY06_01945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020186304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CbtA family protein"
                     /protein_id="HWX13635.1"
                     /translation="MVSGFLLSGLLSGIFAGLLAASFASFAGEPLIERAIAFEGAAAQ
                     AAGELHEPEVVSRTVQRGAGLFTASIVYGAAIGGLFSLVFALTYGRLGDFSPRTLAAL
                     LALLGFIALVLVPELKYPANPPAIGAADTIGQRTALYFALLAISLLATILSATLVPPL
                     AARIGIWNGVLVSAIVFIFAAGLASFVLPAVDEAPSGFPASLLWYFRLASIGARAILW
                     GVLGLAFGAMAQSWMKRGHRA"
     gene            824..1921
                     /gene="nagZ"
                     /locus_tag="VNY06_01950"
     CDS             824..1921
                     /gene="nagZ"
                     /locus_tag="VNY06_01950"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012591251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HWX13636.1"
                     /translation="MPCENIASVGNHFLIGLEPSPVLTGHDRALLSDLQPAGVILFKS
                     NFRHDLPYEDWLQIHARLVADIRVATAREQMLIAIDHEGGRVCRTPVPVTRFSYAAQW
                     AGQAAEVGRAMGRELASLGINLSFAPVLDIHTNPKNPVIGARAFGETPEAVIAAALPF
                     LEAMQSEKVLACGKHFPGHGDTSIDSHVGLPLQTIGLDELRARELRPFAAAIGAGIPM
                     IMTSHILYPAIDPGLPVTLSRRFLTEILRDQLGFFGVAISDDIGMGAMNGFFDNPDSA
                     VGLLESGCDMVMVCAHFTDTGRARDFARAIIKAIDDGRLDVAALERSQARVENLLRRA
                     AKNEVMSLPESLFRAHAKAGPLFDSATVEVV"
BASE COUNT          323 a          613 c          605 g          494 t
ORIGIN      
        1 ctttgtgcat gaattcgtgc atgacgggcg tcgtcttctg gccttcccct gccactgaga
       61 gggggcatgg tgagcggctt tcttttgagc gggctgctgt ccgggatctt tgcgggcctg
      121 cttgctgcga gttttgcatc cttcgcgggc gagcctttga tcgagcgtgc cattgccttt
      181 gaaggcgccg cggcacaggc cgccggggaa ttgcacgagc cggaagtggt gagcagaacc
      241 gtacagcgtg gcgccggact gtttacggcg agcattgtct atggcgcggc tatcggcggc
      301 cttttcagcc tggttttcgc gctcacctac gggcgcctcg gcgatttcag tccgcgcact
      361 ctcgctgcgc tcctcgcttt gctgggcttc atcgccttgg tgctcgtacc ggagctgaaa
      421 tatccggcca atccgcccgc gatcggcgcg gcagacacaa tcggccagcg cacggcgctt
      481 tatttcgctt tgcttgcaat ctctctcctt gccacgatcc tctccgcgac gcttgtccct
      541 cctcttgcgg caaggatcgg gatctggaac ggggttctcg tctcggccat cgttttcatt
      601 ttcgccgccg ggcttgcgtc ctttgtttta ccagctgtcg atgaagcacc ctctggcttt
      661 ccggcgagcc tattgtggta cttccgcctg gcttcgattg gcgcccgcgc gattctgtgg
      721 ggagtgctcg gtctggcctt cggcgcgatg gctcaaagtt ggatgaagcg cggacatcgt
      781 gcttaatctt tatccgccaa gtgaatgatc gaagccaagc cgcatgcctt gcgaaaacat
      841 cgcctccgtc ggaaatcact ttctgattgg cctcgaacct tcgccggttt tgaccggcca
      901 tgaccgcgcg cttttgtccg atcttcagcc tgcgggcgtc attttgttca agagcaattt
      961 ccgccatgac ctcccctatg aagattggct tcaaatccat gcgcggctgg tcgccgatat
     1021 tcgcgttgca actgcgcgcg agcaaatgtt gatcgccatt gaccatgagg gcggccgcgt
     1081 gtgccggact ccggtgccgg ttacgcgctt ctcctatgcc gcccaatggg cggggcaagc
     1141 ggccgaagtt ggccgcgcga tgggccgcga gcttgcttcg cttggcataa atttgagctt
     1201 tgcccccgtg ctcgatatcc ataccaaccc caagaacccg gtgatcggcg cgcgcgcttt
     1261 tggcgagacg ccggaagcgg tcattgcggc ggcgttaccc ttcctggagg ccatgcaaag
     1321 cgaaaaggtc cttgcctgcg gcaagcattt tccggggcac ggcgacacca gcatcgattc
     1381 acatgtcggt ctgccgttac agacaatcgg cctcgatgag ctgcgcgcac gggagctaag
     1441 accttttgct gccgcgattg gcgccggtat tccgatgatc atgacgtctc atattctcta
     1501 tcccgccatc gaccccggct tgccggtcac gctctcgcgc cggttcttga ctgagatttt
     1561 gcgcgatcaa ctagggtttt ttggcgtagc gatctccgac gatatcggca tgggagcaat
     1621 gaatgggttt tttgacaacc ccgactccgc tgtgggcttg cttgagtccg gctgcgacat
     1681 ggtgatggtc tgcgcgcatt ttaccgacac aggccgcgcg cgcgattttg cccgcgcgat
     1741 tatcaaggct atcgatgatg gccgcttgga cgttgcggca ctggagcgtt cgcaagcgcg
     1801 cgtagaaaat ctgctcagac gcgcggccaa gaacgaagtc atgtccctgc cagaaagcct
     1861 ctttcgtgcc catgccaagg cggggccgct ctttgactcg gcaaccgtcg aagtggttta
     1921 ggctcatcgc gccgatgcga agcgggccgc tcaattctca tttatcgact ctgcggttcg
     1981 cggcgattgc gctgctttat acggaaagcc agcggatgcc ggttgcgatt cttgc
//
LOCUS       DATMPU010000086         4254 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_3163256,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000086 DATMPU010000000
VERSION     DATMPU010000086.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 4254)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4254)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4254
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3163256"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            100..579
                     /locus_tag="VNY06_01955"
     CDS             100..579
                     /locus_tag="VNY06_01955"
                     /inference="COORDINATES: protein motif:HMM:NF019430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spy/CpxP family protein refolding chaperone"
                     /protein_id="HWX13637.1"
                     /translation="MPIKMARFWVPLGAAIMGVMACLGAVAAQEQPPGVTSYSVIGGR
                     GAMMCRMDEHIDGQIAYLKTELKITDIQTPQWNIFAQAFRAETEKKAQLCREAMEQSR
                     AGASTNLLDSLKMTEDQMAKRLDSLRAIRAAVQPLYSILTKEQKKTADEIMKSAPNS"
     gene            658..1164
                     /locus_tag="VNY06_01960"
     CDS             658..1164
                     /locus_tag="VNY06_01960"
                     /EC_number="3.1.1.106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012045331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HWX13638.1"
                     /translation="MLEIIVEDITRLDVDAIVNAANSSLLGGGGVDGAIHRAAGPDLL
                     AECRKLGGCTTGDAKITQGYKLPARFVIHTVGPIWRGGARGEEDHLASCYRRSLAIAR
                     EHGLRSIAFPAISTGVYGFPPDRAARIAVASLFDAGREHIIFCCFSESSAEYHQQALA
                     ALGKGNQP"
     gene            complement(1161..1820)
                     /locus_tag="VNY06_01965"
     CDS             complement(1161..1820)
                     /locus_tag="VNY06_01965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003612293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HWX13639.1"
                     /translation="MTEPNPSARFAAFFSPRVAFAALTVAGLAVLLVLYVSKAPAGKN
                     TPIDPACADTAKTAERLRPLVHGKIAALTLASQPKPLPELSFATPDGAKAGLADFKGR
                     TVLLNLWATWCVPCRLEMPALDRLQDKLGAKDFAVVAVNVDTARLDRPKAFLEEIGAK
                     NLTLYADDKAVVLQSLKQAGKVLGLPTTILIGKDGCELGILAGPAQWDSQDAWNLFNA
                     I"
     gene            1854..3248
                     /gene="argH"
                     /locus_tag="VNY06_01970"
     CDS             1854..3248
                     /gene="argH"
                     /locus_tag="VNY06_01970"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HWX13640.1"
                     /translation="MSNKMWGGRFSASPSAIMEEINASIGFDFRLALQDIEGSKAHAA
                     MLARTGILTHVDAEEIGKGLDEIAREIEAGTFKFSCALEDIHMNIEARLAELIGPVAG
                     RLHTARSRNDQVALDFRLWLRDSIAALDQQLHGLQRALAEKALSHAASIMPGFTHMQP
                     AQPVTFGHHLLAYVEMFWRDRLRFRDARTRLNESPLGAAALAGTSFPIDRAMTAKALG
                     FDRPTANSLDSVADRDFVLETLSAAAICAIHLSRLAEEIVLWAGPQFGFVTLSDQFST
                     GSSIMPQKRNPDAAELIRAKAGRIVGALNALLIVMKGLPLAYAKDLQEDKEGTFDALN
                     SLSLCLFAMAGMAGDLTPNTMRMKAAARSGYATATDLADWLVRAQKIPFREAHHITGR
                     LVAIAEAQKKGLEKLSLAEMQAVEPRITEDVFRVLSVENSVRSRKSFGGTAPKNVRTQ
                     ARRWLTRLKREKPV"
     gene            3368..3979
                     /locus_tag="VNY06_01975"
     CDS             3368..3979
                     /locus_tag="VNY06_01975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX13641.1"
                     /translation="MDQSWIELIELCLKVLAVLGAGVWAFTALRVLTNSDTANASLRN
                     SEASIKELELRAKQQAVVSVDIKPAVQRSMDGDGYVITAVVELANRGAQNTTINWKDQ
                     PPAFHVRLAKFDADGKPKYDPPTRFRVPLSLNPRSGAVSHVIRPGATESIPFAFKVAS
                     AGLYLLSFRGAIDALERAEAERRGLQVPEAWTGNKYVLVRGES"
BASE COUNT          931 a         1228 c         1219 g          876 t
ORIGIN      
        1 gtgaggctga cacgggtctt cctcgcacgt gccggagccg tctaacaaga gttgctgcag
       61 gtttattcaa taagtccttc gtgcaaggat cgcatttcga tgccgattaa aatggcaagg
      121 ttctgggtgc cgttaggcgc cgcgattatg ggcgtcatgg cttgtttggg agcagttgct
      181 gcgcaagagc agcctccggg ggtcacctcg tattcggtaa tcggcggacg cggggcgatg
      241 atgtgccgga tggacgaaca tattgacggc cagatcgcct atcttaagac ggaactcaag
      301 atcacggaca tacagacccc gcaatggaat atctttgccc aggcgtttcg ggcggaaacg
      361 gaaaaaaagg cgcagctctg ccgggaagca atggagcaat cccgcgcggg ggcgtccacc
      421 aacctcctcg actctcttaa gatgacggaa gaccaaatgg cgaagcggct cgattctctt
      481 cgtgcaatca gagcggcggt gcaaccgctc tactcgattt tgacaaagga gcagaaaaag
      541 accgccgacg aaatcatgaa aagcgcgcca aattcgtaag accgcgttcg aaggagccgc
      601 gctggccgat agcagttaga atactcggcc cgccattgag gcaagcggca gagtgccatg
      661 ctcgaaatta tcgtcgagga catcacaagg cttgatgtcg atgctatcgt caatgctgcc
      721 aattcctcgc tgcttggtgg aggcggcgtc gacggtgcga tccatcgcgc ggcggggcct
      781 gatctcctcg cggaatgccg caagctcggc ggctgcacta ccggcgatgc caaaatcacg
      841 caaggctaca aattgcccgc gcgttttgtg attcacaccg taggcccgat ctggcgcggt
      901 ggcgcccgcg gcgaggagga tcacttggcg tcctgctacc gccgctcgct cgctatcgcg
      961 cgggaacatg gcctgcgctc gattgctttt ccggcgatct ccacgggcgt ttatggtttt
     1021 cctcccgatc gtgccgcaag aatcgcggtc gcaagtttgt tcgacgccgg tcgggagcac
     1081 attatcttct gctgtttctc agaaagttcg gctgaatacc accagcaggc tctggcggcc
     1141 ttgggaaaag gcaatcagcc ctaaatagcg ttgaacaaat tccaagcatc ctgcgaatcc
     1201 cattgcgcag gcccggccaa aatgcccaat tcgcaaccat ctttgccaat gagaatagtg
     1261 gtcggcagac ccaaaacctt gcctgcctgt ttcaaacttt ggaggaccac ggctttgtca
     1321 tcggcgtaga gcgtcagatt cttggccccg atttcctcga ggaaagcttt tggccggtca
     1381 agccgcgcgg tatcgacatt caccgctacc accgcaaaat ccttcgcgcc gagcttgtct
     1441 tgcaaacggt cgagcgcagg catttccaac cggcacggca cgcaccacgt cgcccaaaga
     1501 ttgagtagca ccgtccttcc tttgaaatcg gccaaacccg cttttgcccc gtcaggcgtg
     1561 gcaaagctca gctccggaag cggttttggc tgggatgcca gtgtcaaggc ggctatcttg
     1621 ccatgaacga gcggcctaag ccgctcggcc gtctttgctg tgtcggcaca cgcaggatca
     1681 atgggggtgt tcttgccggc cggcgctttg ctcacgtata ggacaagcaa aaccgcgagg
     1741 ccggccacgg tcagtgccgc aaaggcaact ctcggcgaaa aaaaggccgc aaaacgcgcg
     1801 ctgggattag gctcggtcat agcgataata acttcttaga cgagcatgcg acgatgagca
     1861 acaaaatgtg gggcgggcgg ttttcggcaa gccctagcgc catcatggaa gagatcaatg
     1921 cctcgatcgg tttcgatttc cggctcgcgc tccaagacat agaagggtcc aaagcgcatg
     1981 ccgccatgct cgcgcgaacc ggcatattga cgcacgtgga cgccgaagaa atcggcaagg
     2041 gcttagacga aattgcgcgt gaaatcgagg caggaacttt caaattttcg tgcgccctcg
     2101 aagacatcca tatgaacatc gaagccaggc ttgcggagtt gatcgggccc gtggctggtc
     2161 ggcttcatac cgcccggtcg cgcaacgatc aggtcgcgct tgactttcgc ttgtggctcc
     2221 gcgattcgat cgccgcactc gatcagcagt tgcacggtct gcaacgagcg ctcgccgaaa
     2281 aagcgctttc gcatgccgca agcatcatgc cgggctttac gcatatgcag ccagcccagc
     2341 ctgtgacctt tggccatcat ctcttggcct atgtggagat gttttggcgc gaccgtctaa
     2401 ggtttcgcga cgcgagaaca cgccttaatg aatcgccgct cggcgccgcc gcgttggccg
     2461 gaacctcctt tcccatcgac cgggcaatga ccgcgaaagc actcggcttc gaccggccga
     2521 ccgcgaattc gctcgatagc gtcgccgacc gggactttgt gctcgaaact ctcagcgccg
     2581 cggcaatctg cgccattcat ctctcccgcc tcgccgagga gatcgtgctg tgggcagggc
     2641 cacagtttgg ctttgtcacc ttatccgacc aattttcgac cggctcgtcg atcatgcccc
     2701 aaaagcgcaa tcccgacgcg gcagagctga tacgcgccaa agccggccgg atcgtcggtg
     2761 cactcaatgc cttgctcatc gtcatgaaag gcctgccgct cgcttacgcg aaggatttgc
     2821 aggaagacaa ggaaggcact ttcgatgccc tgaattcttt gtcactatgc ctattcgcga
     2881 tggccggaat ggcgggcgat ctcacgccga acacaatgcg catgaaagcc gctgcgcgtt
     2941 cgggttatgc gacggctacc gaccttgccg attggctggt cagagcgcaa aaaatcccgt
     3001 tccgcgaggc acaccatatc acgggccgcc tcgtcgccat cgccgaggcg caaaagaagg
     3061 ggcttgaaaa gctatcgctc gcggaaatgc aggcagtaga gccgcgaatc actgaggatg
     3121 ttttccgcgt gcttagcgtc gagaactcgg tgcgcagccg aaaaagtttt ggcggcaccg
     3181 cgccaaaaaa cgtgcgcact caggcgcgcc gctggcttac ccggctcaaa cgggaaaaac
     3241 ctgtgtaaaa cggcacgagc gttagatttt gtctctcatt ggctgtcctc ggctgtaggc
     3301 tccttataga tgcgcgccgg ttgcgcccac acgggcggta tggcgctttc atgcgaggat
     3361 caaaccgatg gatcagtcat ggattgaact tatcgagctc tgcctcaaag tccttgcggt
     3421 gcttggtgca ggcgtctggg ctttcactgc gctgcgcgtc ctaacgaaca gcgatactgc
     3481 caatgcttcg ttgcgcaatt cggaagcaag catcaaggag cttgaactgc gggcaaaaca
     3541 gcaagccgtc gtttcagtcg acatcaaacc agcggttcaa cgcagcatgg acggcgatgg
     3601 ttatgtcatt actgcggtcg tggagctcgc caaccgcggc gcccagaata cgaccatcaa
     3661 ttggaaagat cagccgccag ctttccatgt gaggctcgcc aaattcgatg ccgatgggaa
     3721 accaaaatat gatccgccca cgcggtttcg tgtcccactc agcctcaatc ccagatccgg
     3781 cgccgtctcc catgtcattc gccccggcgc gaccgagtcg attccgttcg cattcaaagt
     3841 cgcctcggca ggcctttatt tattgtcatt tcgcggcgct atcgatgctt tggaacgtgc
     3901 cgaggcagaa aggcgcggct tgcaagtgcc tgaagcctgg accggcaaca aatatgtttt
     3961 ggtgcgcggc gagagctgaa acttgaggga ggggcaaaca gttggcgact gcaaagtgtt
     4021 gctaatagtc caagcagaga cgcacgctgc cctgggcgga tttggcgtgg cttgcaggaa
     4081 tgaagtgatg gtcttggcaa attcgaattg gcgccttcgt ttgcaaaggt aaaatcaagt
     4141 gagatgagcc aagatgctga ccagagcctg acagcgtgcc accacaagag ccgccttcac
     4201 aacccaaaat ttcaacgtct acctattcta ccgagctttc ggctgatgtt gcgg
//
LOCUS       DATMPU010000087         3656 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_1614360,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000087 DATMPU010000000
VERSION     DATMPU010000087.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 3656)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3656)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3656
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1614360"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            44..241
                     /locus_tag="VNY06_01980"
     CDS             44..241
                     /locus_tag="VNY06_01980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013845026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HWX13642.1"
                     /translation="MDWNRIEGNWKQLKGKLKEQWGHLTDDDLDKINGRREQLEGKIQ
                     ERYGITRDAARKDIDDWMARQ"
     gene            474..629
                     /locus_tag="VNY06_01985"
     CDS             474..629
                     /locus_tag="VNY06_01985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020487478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3309 domain-containing protein"
                     /protein_id="HWX13643.1"
                     /translation="MLSTILMVVLILLLVGALPAWPYSASWGYGPGGLLGTVLVIVVI
                     LALLGRV"
     gene            complement(1035..2441)
                     /gene="leuC"
                     /locus_tag="VNY06_01990"
     CDS             complement(1035..2441)
                     /gene="leuC"
                     /locus_tag="VNY06_01990"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007598823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HWX13644.1"
                     /translation="MAKPRTLYDKIFDDHLVHTEPDGASLIYIDRHLVHEVTSPQAFE
                     GLRLSGRRVRAPQKTLAVVDHNVPTTDRSKGIDDPESKIQVDTLAHNAREFGVEYFNE
                     RDSRQGIVHIIGPEQGFTLPGTTIVCGDSHTSTHGAFGALAHGIGTSEVEHVLATQTL
                     IQTKAKNMRVTVDGALGDGATAKDIVLAIIGEIGTAGGTGHVIEYAGKAIRALSMEGR
                     MTVCNMSIEAGARAGLIAPDEKTFAYLKNRPKAPKGADWDAAMRYWEGLRSDPDAVFD
                     KEIRLDASKLPPLVTWGTSPEDVVTIEGFVPCLDAATNETQKAKIERALNYMGFRGGE
                     RITDVSIDRVFIGSCTNGRIEDLRAAAKMVEGKHVAASVNAMVVPGSGLVKKQAEAEG
                     LDRIFLAAGFEWREPGCSMCLAMNPDKLAPGERCASTSNRNFEGRQGFRGRTHLVSPA
                     MAAAAAIAGHFVDVREWH"
     gene            2566..2967
                     /locus_tag="VNY06_01995"
     CDS             2566..2967
                     /locus_tag="VNY06_01995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020174808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HWX13645.1"
                     /translation="MRPPVAPYLTVSPALAAITFYTTVFGARQKSIMPSFDGMRIMHC
                     ELLINGGSVMIADAFPEFGQTRVPMPGDLATASVSLEYAEAKTVDEIFAKATSLGAKG
                     ETNPTNSFWGTRVATFRDPFGHRWILNAPLA"
     gene            complement(3000..3464)
                     /gene="rplS"
                     /locus_tag="VNY06_02000"
     CDS             complement(3000..3464)
                     /gene="rplS"
                     /locus_tag="VNY06_02000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020174811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HWX13646.1"
                     /translation="MNIIAELEAAQAAKLLGGKTIPAFAPGDTVVVNVKVKEGDRIRV
                     QAYEGVCIARAGSGLNESFTVRKISYGEGVERVFPVYSPNIDSIKVVRRGKVRRAKLY
                     YLRDRRGKSARIAEKMETPAAKSAREAAKKEAKAAVKSKKAASRAGSPPAAS"
BASE COUNT          800 a         1052 c          998 g          806 t
ORIGIN      
        1 cggcgctcat acgttgccgc ttgtttacct aaaggagacc atgatggatt ggaaccgcat
       61 cgaaggaaac tggaaacaac tgaaaggcaa gctcaaggag caatggggcc atctaacgga
      121 cgacgatctc gacaagatca acggccgccg ggagcagctc gaaggcaaaa tccaggagcg
      181 ttacggcatc actagggatg ctgcgcgcaa ggacatcgac gactggatgg cgaggcagta
      241 atctcgttga cgaagggccg catcgtcggt gcttgatgcg gccccgttcg cggtgcgaaa
      301 ccttatgcat gaattggagt aaggtgcgcg gaaaacgagc tcaggaggga tacagcgaca
      361 agccctacac ttcactcact gcttttgaac cgatttgaga aacaatcttg tgtgtacgga
      421 actttggctt tgcgcagtga ttacgatgca ggaaacggtc aaggagtaat caaatgcttt
      481 ccacgatctt gatggttgta ctcatcctcc tgctagtcgg agcactgccc gcgtggccgt
      541 acagcgcctc ctggggttat gggccaggtg ggttgctcgg caccgtgttg gttatcgttg
      601 tgattctggc cctcctaggt cgcgtttaga tcctggacta cgggcttaat cttttgcgag
      661 gacggggtga tccgcgtctc tcgcaaaact catgcgtgaa cgcggtatga gatagagcaa
      721 gaaaagagac gcaaagctgt cctgatgagg caggccttca taaggccacg ctcactcact
      781 aacgatttag cgccaggagg cagccaatgg cggctgcttc cactgctcaa acaaattcct
      841 gctcgcaatt tcaattcctt cccgccaaac aatcactatc ttattgtcag cgcagaaaga
      901 tttatgagtt ctgaccctag cttctcttga ttcgagacac cccctttggc tcgaccttca
      961 tcaatcgaaa gttttgattt ttgcacgaga cgggaaatgc ccctggcgca attcgttcgc
     1021 cgatctgaaa taactcagtg ccactcccta acatccacaa aatgcccggc gattgccgct
     1081 gccgccgcca tggccggtga gacgagatgg gtgcggcccc taaaaccttg acggccctca
     1141 aaattgcggt tggaagtcga ggcgcagcgt tcgccgggtg cgagtttatc cggattcatt
     1201 gcaagacaca tcgagcagcc aggttcacgc cactcaaatc ccgcagcaag gaaaatccta
     1261 tcaagcccct cggcttcggc ctgtttcttg acgagccccg agcccggcac gaccatggcg
     1321 ttgacgcttg ccgccacatg cttgccttca accattttag cggcggcgcg caaatcctcg
     1381 atcctgccat tggtgcagga gccaataaaa acccggtcga tggaaacatc ggtgattctt
     1441 tcgccgcccc taaaacccat gtaatttaac gcgcgctcaa ttttggcctt ctgagtctcg
     1501 ttcgtcgcgg cgtcaagaca aggtacaaag ccttcgatcg tcaccacatc ttccgggctc
     1561 gtgccccaag tgacgagcgg cggtaatttg gatgcatcga ggcggatttc cttgtcgaag
     1621 acggcgtccg gatcggagcg caaaccttcc caatagcgca tcgctgcgtc ccagtccgca
     1681 cctttcggcg ctttggggcg gtttttcaga taagcaaagg ttttttcatc gggcgcaatc
     1741 aaaccggctc gggcgccagc ctcgatcgac atattgcaga cggtcattct tccttccatc
     1801 gacaaagcac ggatcgcttt cccggcatat tcgatcacat ggccagtgcc tcccgccgtg
     1861 ccgatttcgc caatgattgc aagcacaatg tccttggcag tcgcaccatc gccaagtgcg
     1921 ccatcaacgg tgacgcgcat attcttggcc ttggtctgga tcaacgtctg ggtcgcaagc
     1981 acatgctcga cctcggacgt gccaatgcca tgggcgagcg cgccaaaggc gccatgggtc
     2041 gaggtatggc tatcgccaca gacgatcgtc gtgccaggca aggtaaagcc ttgctcaggt
     2101 ccgataatat ggacgatgcc ttgacgcgag tcgcgctcat taaaatattc gacgccgaat
     2161 tcgcgtgcgt tgtgggcaag cgtgtcgact tggattttgc tttcgggatc atcgatgcct
     2221 tttgagcgat ctgtggtcgg cacattatga tcaacgaccg cgagggtttt ttgcggcgcc
     2281 cggacgcgcc gcccggaaag ccgcagcccc tcaaaagcct gggggctcgt cacctcatgc
     2341 acaagatgac ggtcgatata gatcaaactc gcgccatcgg gttcggtatg gacgagatga
     2401 tcatcaaaga tcttgtcgta aagcgtgcga ggtttggcca tgaggcgggt actgtgctta
     2461 agcagctctt gcaaaaaaac caattcggat ggcagttctt agcggcaaga cgcggcgacg
     2521 ggaagtcccc ccgcgatatc gacgaatgtt tacgcggaga atgccatgcg cccgccggtt
     2581 gcgccctatc tgaccgtttc ccctgcccta gcggcgataa ccttctacac gaccgttttc
     2641 ggagcgcgcc aaaaatcgat catgccctcg ttcgacggca tgcggatcat gcattgcgag
     2701 cttttgatca acggaggctc ggtcatgatt gccgacgctt ttcccgaatt tggccagacc
     2761 cgagtgccta tgccaggcga ccttgcgacc gcttcggtta gcttggaata tgcagaggcc
     2821 aagacagtcg acgaaatctt cgcaaaagcg acaagtcttg gcgcgaaagg cgaaaccaat
     2881 ccgaccaatt cattttgggg gacgagggtt gcgacctttc gcgatccgtt tggccatcgc
     2941 tggatcttga atgccccgct ggcttgagcc cgcgcgatct ccccccaatt cgccgggccc
     3001 taactggcgg ccggaggtga accagcgcgg gaagcagcct tctttgattt gacagccgcc
     3061 ttggcttctt ttttggcagc ctcgcgcgca gacttggcgg caggcgtctc cattttctcg
     3121 gctatgcgcg ctgacttgcc ccgccggtcg cggagataat aaagctttgc gcgccgcacc
     3181 ttgccccgcc gcactacctt gatcgaatcg atatttggcg aatacaccgg aaacacgcgc
     3241 tcgacgcctt cgccatagga aatcttgcgt accgtaaagc tctcattgag accgctgccg
     3301 gcgcgggcaa tgcagacccc ttcataggcc tgcacgcgga tgcgatcacc ttccttcacc
     3361 ttgacattca cgacgacagt gtcgcccggc gcaaacgcgg gaattgtctt tccgcccaaa
     3421 agcttggcgg cctgcgcggc ctctagttcc gcgatgatat tcattagact aaacctctca
     3481 cgccgccgga ccttggtcct gcgcgcgtcg cgatgccctc gtttgaattg gaaagccaac
     3541 ctctacacga cgggaagttg cttgtcgagc ccgggcttaa ggaaaccgcg gccaagaaac
     3601 aaacggagaa atggagaaat aatgtcacgg atgatttttt ttggccgcgg gagcct
//
LOCUS       DATMPU010000088         9401 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_4092559,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000088 DATMPU010000000
VERSION     DATMPU010000088.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 9401)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9401)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9401
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4092559"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..165)
                     /locus_tag="VNY06_02005"
     CDS             complement(<1..165)
                     /locus_tag="VNY06_02005"
                     /inference="COORDINATES: protein motif:HMM:NF024750.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Arm DNA-binding domain-containing protein"
                     /protein_id="HWX13647.1"
                     /translation="MRRREILTALSVQRLSKPGRHADGGNLYLYVDKTGGKKWTFFYQ
                     LAGRQHEMGLG"
     gene            complement(321..3764)
                     /gene="pyc"
                     /locus_tag="VNY06_02010"
     CDS             complement(321..3764)
                     /gene="pyc"
                     /locus_tag="VNY06_02010"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012590395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004736 - pyruvate carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HWX13648.1"
                     /translation="MRKIRRLLVANRSEITIRVFRAAAELGIGTIAIYAEEDKLSLHR
                     FKADEAYQLGTGKGALAAYLSIEDVIRVAKAAKADAIHPGYGFLSESPEFAEACAAAG
                     ITFIGPSPQVLRALGNKLAARNLAASCGVPVMPASPPLPDDPQEIRRLAGEIGYPLML
                     KASWGGGGRGMRQIENEGKVLDSVLSAKREAKAAFGKDEVFLEKLVLRARHVEVQILG
                     DTNGNLVHLFERDCSIQRRNQKVIERAPAPYLDDNARHRLCEAALKIGKAARYESAGT
                     VEFLMDSDTGEFYFIEVNPRIQVEHTVTEEVTGLDIVKAQIRIAEGKRIGVLEETGIP
                     PQDLIRLSGHALQCRITTENPENNFIPDYGRITAYRGAMGFGIRIDGGTAYSGAVVTR
                     FYDPLLEKITSWAPSAEEAIRRMNRALLEYRIRGVATNLAFLQNIINHPKFIASNYTT
                     CFIDETPSLFEFKKRKDRATKLLTWIADVTVNGHPETRGRARPPANLREPALPQLPEI
                     PIQDGARQLLEKLGPKAFAEWMRAETRLLMTDTTMRDAHQSLLATRMRTKDIAAVAGL
                     YAKGLPNLLSLECWGGATFDVAMRFLSEDPWERLALIRERAPNLLTQMLLRGANGVGY
                     TNYPDNVVKYFVRRAAAGVDLFRIFDCLNWVENMRVAIDAVCETGKLAEGGICYTGDI
                     LDPNRAKYSLTYYVGLAKELERAGCHILAIKDMAGLLRPSGARLLVKALREESGLPLH
                     LHTHDTSGISAATVLAAAEAGVDAADAAIDTMSGTTSQPCLGSIVKALRHCDRDTGLD
                     SAVIRKLSTYFEAARLQYAAFESDLKTGTAEVYLHEMPGGQFTNLKQQARALGLERHW
                     DEVAAAYRAANDLFGDIIKVTPSSKVVGDMALMMVAQDLTPQDVLDPKRDIAFPGSVV
                     EMFRGDLGQPPGGWPKTLQAKILKGEEPITVRPGSLLAPADLGAAKAAAEKACGRQIS
                     EDELASYLMYPKVFAEYAATNRRYGPVSVLPTPVFFYGMEPGNEVAIEIEPGKTLVVV
                     LMTIGETDEEGQVKVFFELNGQPRIIWVRNRAAVAKTAPRRKAEDGNPSHIAAPMPGS
                     VATIAVRLGQEVEAGDVIATLEAMKMETVLHAPRDGKIAEILVAPGQQIDARDLLLRI
                     E"
     gene            3970..5010
                     /locus_tag="VNY06_02015"
     CDS             3970..5010
                     /locus_tag="VNY06_02015"
                     /inference="COORDINATES: protein motif:HMM:NF021423.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2125 domain-containing protein"
                     /protein_id="HWX13649.1"
                     /translation="MSSEALAWKQRLRAYSWLIVLLAILAMAPIGWSIFWFVKSRAAA
                     AAVTAWMAQEARQGRVWSCPSQTIGGFPYNIAISCQNLAFQGEAFGKTLSGTIHALRA
                     TSPLLRNDNVLVKLEPPLTAKSSGGDLDISAQWSDLLVELEGTPGMIEQLALMGSQIG
                     LQGVALGTDATGSTFGDVNGYFARSAGRQDQAYDVVVSFHQGQIPALNSFLDTNVPIA
                     LQFEAVMTPGFPGGAASFADTLEKWRAANGRLDVTFASLTSGPVDFNAKGGLGIDSGH
                     RVEGKLDARFAGLEKALRNLNVDPTLLSFGQAISGFLGGGRDGLKLPVIFSDGFLRIG
                     PVRTGVQVPPLY"
     gene            complement(5039..5986)
                     /locus_tag="VNY06_02020"
     CDS             complement(5039..5986)
                     /locus_tag="VNY06_02020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019400262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate/arogenate dehydrogenase family
                     protein"
                     /protein_id="HWX13650.1"
                     /translation="MPDGLGAPFDEPLFQRLALIGMGLIGSSLAHVCRRKGLVREIIA
                     ADASPEVRSQVAGLALANRVAETPSAAVEGADLVILCVPVGAMGRIAQEIAPYLLRGT
                     ILSDVGSVKTAIIKAVAPHVPATIHFVPAHPVAGTEQSGPKSGFATLFLNRWSILTPP
                     EGTSSKAVARLSSFWTGAGANVEVMQAAHHDLVLAITSHLPHLIAYNIVGTAAGLEEV
                     TQSEVIKFSAGGFRDFTRIAASDPTMWRDIFLNNKEAVLETLGRFNEDLSALQRMIRN
                     GDGKGLFDLFARTRAIRRGIIEQGQETAAPDFGRRAGET"
     gene            complement(5979..7109)
                     /gene="hisC"
                     /locus_tag="VNY06_02025"
     CDS             complement(5979..7109)
                     /gene="hisC"
                     /locus_tag="VNY06_02025"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020187168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HWX13651.1"
                     /translation="MRKIALSRTDARPAPRASVLSIDPYVPGKSAALGAAKGFKLSAN
                     ETPLGPSPRARDALRDYADHLQDYPDRAAIALRAALGAKHGFNPEKIVCGNGSDEILH
                     LLASVYIGPGDEGIFTEHGFLVYKIAILAAGGTPVVAGETNLTADVEKILAKVGPRTK
                     MVFLANPNNPTGTYLSSAEIQRLALSLPRHVLLVIDAAYAEYVTRADYSPSHELVENS
                     ENIVMTRTFSKIYGLAGIRLGWCYAPRDVCDALNRIRGPFNINGAANAAGIAALQDAA
                     HIAKAVAHNETWLAWLTDKISALGIKVTPSVGNFLLLHFADKTRAMEADRFLTRRGLI
                     LRAVSAYGLPHCLRLTVGTEEANRLVAAALKDFAAIESPARA"
     gene            complement(7132..7983)
                     /locus_tag="VNY06_02030"
     CDS             complement(7132..7983)
                     /locus_tag="VNY06_02030"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012383318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046417 - chorismate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HWX13652.1"
                     /translation="MVDLSPHDTLDELRCEIDRIDAALHFLLMERGKIIHRLIEAKAR
                     QGGGSAFRPGREAEMMRRLVSRHKGLLPLDTVEGIWRIIISTFTFVQSNYSVHTDISG
                     GDAAMRDSCRFHFGFTVPYVAHQSAAGVIEAVTASSGDLGVVQAEHHELAGPWWRGLA
                     APDAPKIIARLPFVERPDHPAGTPIFVIAKPLAEALVRDVVLYDVTFPKGGEDFASAC
                     AEHGFEVLGQSQDDAGRSLMVAANGAASALREEIARAGTADAEIAEIGSHAARFDLSS
                     AESVAAQ"
     gene            complement(8296..9147)
                     /gene="dapD"
                     /locus_tag="VNY06_02035"
     CDS             complement(8296..9147)
                     /gene="dapD"
                     /locus_tag="VNY06_02035"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012386239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008666 -
                     2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HWX13653.1"
                     /translation="MLDLERIINAAFETRASIDARTKGDTRDAVDTALSLLDTGKLRV
                     AEKIEGASGLGSWRVNEWLKKAVLLSFRLNEMSAISGGPGGTSWWDKVPSKLAGWGPA
                     EHAAAGFRSVPNCVVRRSAYIAPGVVLMPSFVNLGAYVDSNTMVDTWVTVGSCAQIGK
                     NVHLSGGVGIGGVLEPLQANPTIIEDECFIGARSEIVEGVIVGQGSVIAMGTFISAST
                     KIIDRATGEVRLGYVPPYSVVVSGNLPGKPLASGAPGPSLYCAVIVKTVDAKTRAKTS
                     INELLRD"
     gene            complement(9216..>9401)
                     /locus_tag="VNY06_02040"
     CDS             complement(9216..>9401)
                     /locus_tag="VNY06_02040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012386238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HWX13654.1"
                     /translation="IIPHQKGMTTVLVVPKPGQKVHREAFETAHEAAALHIDYFTSDL
                     SQFLNGFAPPCLNSSGP"
BASE COUNT         1931 a         2660 c         2829 g         1981 t
ORIGIN      
        1 cccgaggccc atttcatgct ggcggccggc gagttggtaa aaaaatgtcc attttttgcc
       61 gccggttttg tcgacgtaaa gatacagatt cccgccgtcc gcatggcgtc cgggcttgct
      121 cagccgttga accgacaacg ccgttaggat ttctcgccgc ctcattggtg ctcactctga
      181 gaacctagtg caagtcctag tccaaacgcc ggattgtgtc aacctgatgt ggacatgttt
      241 gcacagttaa ggcatggatc tgcttggttt ttaaaggatt tttgcacagt cctggacagc
      301 ggcggacagg ttccggacgg ctattcgatc cgcagcaaca aatctctggc gtcgatctgc
      361 tggccggggg ctaccaaaat ttccgcaatt tttccgtcgc gcggggcgtg cagcacggtc
      421 tccattttca ttgcttccaa tgtggcgata acgtcgcctg cctccacctc ttgtccaagg
      481 cgcaccgcga tggttgcgac cgagcctggc atgggtgctg cgatatggga cggattgcca
      541 tcctcggctt tgcgccgtgg agcggtcttc gccaccgccg cgcgattgcg cacccagata
      601 attcgcggct ggccgttcaa ttcaaaaaag acttttactt gcccttcttc gtcggtctcg
      661 ccgatcgtca tcaggacaac gacaagcgtc ttgccgggct cgatctcgat ggcaacctcg
      721 ttgccgggct ccatgccata aaaaaacacg ggcgttggca gtacggagac gggtccgtaa
      781 cggcggtttg tcgccgcata ttcggcgaag acttttggat acatgagata agaggcaagc
      841 tcgtcttcgc tgatttggcg cccgcacgcc ttttcggcgg cggccttggc agcgccaaga
      901 tcggccgggg cgagcaacga gcctggccgc acagtgatgg gttcttcgcc cttaagaatc
      961 tttgcctgca aagttttggg ccatcctccc ggtggttgac cgagatcgcc cctaaacatt
     1021 tcgacgacgg aacctggaaa ggcgatgtcg cgcttggggt cgagcacatc ttgcggtgtc
     1081 agatcctggg cgaccatcat cagcgccatg tcgccgacca cttttgatga aggcgtcact
     1141 ttgatgatat cgccaaaaag atcatttgcg gcgcgatagg ccgcggcgac ctcgtcccag
     1201 tgcctttcga gaccgagtgc gcgcgcctgc tgcttgagat tggtgaactg gccgcccggc
     1261 atttcatgta agtagacctc ggcggtaccg gttttgagat cgctttcaaa agccgcatat
     1321 tgcaagcgcg ccgcttcaaa ataggtgctg agcttgcgga tcaccgcgga atcgaggccc
     1381 gtgtcgcgat cgcagtggcg caaggccttg acgatcgagc cgaggcaggg ctgcgatgtc
     1441 gtgcccgaca ttgtgtcgat tgcggcatct gcggcatcga ctccggcctc cgccgccgcg
     1501 agcacggtcg ccgccgatat acccgacgtg tcatgggtgt ggaggtgcag cggcaagcca
     1561 gactcctcgc gcagggcttt gacgaggagg cgcgcgccag aaggccgcag gagccccgcc
     1621 atgtctttga tggcgaggat atggcagccc gcccgctcga gctccttggc gaggccgaca
     1681 taataggtga gtgaatattt ggcgcggttg gggtcgagga tgtcgcccgt gtagcagatc
     1741 ccaccttcgg cgagtttgcc ggtctcgcac acggcgtcga ttgcaacgcg catattctcg
     1801 acccagttga ggcaatcgaa aatgcggaag agatcaactc ccgcggccgc ccggcggaca
     1861 aaatatttca ccacattgtc gggataattg gtgtagccaa cgccattggc gccgcgaagc
     1921 agcatttgag tcagaagatt tggcgcccgc tcgcgaatca gtgcaagacg ctcccaaggg
     1981 tcttccgata aaaatcgcat cgcgacatca aaggtcgcgc cgccccagca ttcgagggag
     2041 aggaggttcg gcaagccttt ggcatagagt cctgcaacgg cggcgatgtc cttcgtgcgc
     2101 atacgcgttg cgagcaggga ttgatgcgca tcgcgcatgg tcgtgtcggt cataagcaaa
     2161 cgggtctcgg cgcgcatcca ttcggcaaaa gctttaggcc cgagcttttc gagcaattgc
     2221 cgtgcgccat cttgaatcgg aatttcaggc agctgcggca gggcaggttc ccgcagattg
     2281 gccggcggcc gcgcgcggcc gcgggtttcg ggatggccat tcacggtcac atcggcgatc
     2341 caagtcagaa gttttgttgc ccggtctttg cgtttcttga actcgaagag agatggcgtc
     2401 tcgtcgataa aacacgtggt gtagttgctg gcaataaatt ttggatgatt gataatgttc
     2461 tggagaaagg cgagatttgt tgcgactccg cgaattcggt attcgaggag ggcgcggttc
     2521 atcctgcgaa ttgcttcctc ggccgatggg gcccaggaag tgattttttc aagcaaaggg
     2581 tcatagaagc gcgtgacgac cgcgccggaa taggctgtgc cgccgtcgat tctaatgcca
     2641 aagcccatgg cgccgcgata ggcggtgatg cggccgtagt cgggaataaa gttgttttcc
     2701 ggattttccg tcgtgatacg gcattgcagg gcatgacccg aaagccggat gaggtcctgg
     2761 ggcggaatcc ctgtttcttc gagcacgcca atacgcttgc cttcggcgat gcgaatctgt
     2821 gctttcacaa tatcaaggcc cgtaacctct tcggtcactg tgtgctcgac ctggatgcgc
     2881 ggattgactt cgatgaaata aaattcacct gtgtccgaat ccatcaaaaa ctcaaccgtt
     2941 ccggcgctct cgtatcgggc ggccttgccg attttgagtg ccgcttcgca gaggcggtgg
     3001 cgcgcgttgt catcaagata aggggcgggt gcgcgctcga tcactttctg gttgcggcgc
     3061 tggatcgaac aatcgcgctc aaaaagatga acgagattgc cgtttgtgtc tccgaggatt
     3121 tgaacttcga cgtgccgcgc ccggagcaca agtttttcga gaaaaacttc gtccttgccg
     3181 aaggcggcct ttgcctccct cttggcgctc agaacggaat cgaggacttt gccttcgttc
     3241 tcgatctggc gcatgccacg cccgcctcct ccccaggagg ccttgagcat gaggggatag
     3301 ccgatctcgc cggcaagacg cctaatctct tgcggatcgt cgggcagagg cggggacgcc
     3361 ggcatgaccg gcacgccgca ggaggccgcg agattccgtg cggcgagctt attgccaagg
     3421 gcgcgcaaaa cctgtgggga cgggccgatg aaagtgatgc ccgccgctgc gcaggcttcg
     3481 gcgaattccg gactctcgga aagaaaacca tatccgggat gaatcgcatc ggccttggct
     3541 gctttcgcaa cccggatcac gtcttcgatc gaaagatagg ccgcaagcgc ccccttgccg
     3601 gtgccgagct gataggcctc atccgcctta aaacggtgaa gcgaaagctt atcctcttcc
     3661 gcatagatgg caatcgtgcc aattccaagt tctgctgccg cgcgaaagac ccggattgta
     3721 atctcggagc ggttggcgac gagcaggcgg cggatcttgc gcatggacac ttgatttggt
     3781 tgaggaggtg cttttcattt tcctgcaaat catgcaccac gggcccggtc aacaatgagg
     3841 cgatggcagg agccggcgag cttccgttgg gggggtccgt tctggccgcc cagccttggc
     3901 ccggcgccgg tcagcatgcc acgaaaaaga aacgtttcgt ttgaacaaaa ttctgggaga
     3961 aaacacctta tgagttctga agccctcgcg tggaagcagc gcctgcgcgc ctattcctgg
     4021 ctgattgtgc tcctcgccat tctcgcgatg gcgccaatcg gctggtcaat tttctggttt
     4081 gtcaaatcgc gcgcggccgc cgcagcggtg acagcgtgga tggcgcagga agcgcggcaa
     4141 ggccgtgttt ggtcctgccc ttcgcagaca atcggcggtt ttccttataa cattgcaatt
     4201 tcttgccaga atttggcgtt tcaaggcgaa gcctttggca agacattgag cggaaccata
     4261 cacgccctcc gcgcgacctc gccgcttctg cgtaacgaca atgtgctcgt gaagctcgag
     4321 cctccgttga ctgcaaaaag tagcggcggc gatctagata tctcggcgca atggagcgac
     4381 ctgctggttg agctcgaagg cacaccgggc atgattgaac agcttgcgct catgggtagc
     4441 caaattgggc tgcaaggcgt ggctctagga acggacgcta cgggatcgac ttttggagat
     4501 gtaaacggct atttcgccag gtccgccggc cggcaggacc aagcctatga tgtcgtggtg
     4561 tcgttccatc aggggcaaat tccggcgctc aatagcttct tggacacaaa cgtcccaatc
     4621 gcgctgcaat tcgaagccgt aatgacgccg ggctttccag gcggcgcggc aagttttgca
     4681 gacacgctcg aaaagtggcg cgcggcgaat ggccggcttg atgttacttt tgcctcgctg
     4741 accagcggcc cggtagactt caacgccaaa ggcggtctcg gtatcgacag cgggcaccgt
     4801 gttgaaggca agctcgatgc gcgttttgcc ggactagaga aggcgctccg gaatctaaat
     4861 gttgatccaa cccttctgag cttcggacag gcgatttctg ggtttttggg cggtggaagg
     4921 gacggcctga aacttccggt aattttttcc gacggttttc ttcggatcgg tccggttcgc
     4981 acgggggtcc aggtaccgcc gctctactaa tcgcttaagg ccacctgcaa agggggcgtt
     5041 acgtctcgcc cgcccggcgg ccaaagtccg gcgcggccgt ttcctgtcct tgctcaatga
     5101 tgccgcgccg gatcgctctt gtcctcgcga agagatcgaa gaggcccttt ccatcgccat
     5161 tgcggatcat ccgctgcagc gcgctcaaat cctcgttaaa ccggccgagc gtttcgagaa
     5221 cagcttcctt gttgttgagg aaaatatccc gccacatcgt cgggtcggaa gctgcgatcc
     5281 gggtgaaatc gcgaaatcct cctgcagaga atttgatcac ctcggactga gtgacttctt
     5341 caagaccggc ggccgtgccg acgatattat aggcgatgag atggggtaaa tggctcgtga
     5401 tcgcgagcac cagatcgtga tgcgcagcct gcatgacttc cacattggcg cccgcgccgg
     5461 tccagaagga gctaagccgt gccacggcct tggagcttgt tccctcaggc ggggtgagga
     5521 tgctccagcg gttaagaaaa agcgtcgcaa atccgctttt agggccggat tgctcggtgc
     5581 cggccaccgg atgcgccggg acgaaatgaa tcgtggcggg aacatgcgga gcgacggcct
     5641 tgatgatcgc ggttttgacc gagccgacgt cagaaaggat tgtgccgcgc agaagatagg
     5701 gcgcgatttc ctgcgcgata cggcccatcg cgccaacggg aacgcagaga atcacaaggt
     5761 cagcgccttc gaccgcggcg gatggcgttt cggcgacgcg attcgccaga gcgaggccag
     5821 cgacttgcga gcggacttct ggggatgcgt cggccgcaat gatctcgcgc acaagcccct
     5881 tacgccggca cacatgcgcg agagaagagc caatgagtcc catgccaatg agtgcaaggc
     5941 gctgaaacaa aggctcgtcg aagggcgccc ctagaccgtc aggcacgtgc cggactttcg
     6001 atagctgcaa agtccttgag ggccgcggcg acgagacggt ttgcttcctc ggtgccgacc
     6061 gtgagccgca ggcaatgcgg cagcccatag gcggaaacgg cccgcaggat caagccccgg
     6121 cgcgtgagaa agcgatccgc ttccatcgct cgcgtcttgt cagcgaaatg cagtaggaga
     6181 aagttcccaa cgctcggcgt gactttgatg ccgagcgcag aaattttgtc cgtgagccag
     6241 gcgagccaag tctcgttgtg ggcgacagcc ttggcgatat gagcggcgtc ttgcagcgcc
     6301 gcgatcccag ctgcattggc cgcgccattg atattgaaag gcccccgaat ccgattgagc
     6361 gcatcgcata catcacgcgg cgcatagcac cagccgagcc taatgccggc gagaccatag
     6421 attttcgaaa acgttctggt catcacaata ttttcgctgt tttcgacaag ctcatggcta
     6481 ggcgagtaat cggcgcgcgt cacatattcg gcataggccg catcgataac aagaaggaca
     6541 tggcgcggca ggcttagcgc gagccgctga atttcggcgg aagaaagata agtcccggtc
     6601 ggattattcg gattggcaag gaagaccatt ttggtacgag gaccgacctt ggcgaggatt
     6661 ttttcgacat cggctgtgag atttgtttcc cccgcaacga cgggcgttcc tcccgccgcg
     6721 agaatggcga ttttatagac gagaaaacca tgttcggtga atatgccttc gtcacccggg
     6781 ccgatataga cggaggcaag gagatggagg atttcatccg agccattgcc gcacacaatt
     6841 ttttccggat taaaaccatg ctttgcgcca agtgccgccc gcagcgctat ggctgcgcgg
     6901 tcgggatagt cctggagatg atccgcgtag tcgcgcagcg catctcttgc gcgcggtgat
     6961 ggcccaagcg gcgtttcgtt agcggaaagc ttgaaccctt tcgctgcgcc tagagcggca
     7021 cttttgcctg gaacataagg gtcaatgctc agaacagagg cgcgcggggc tggccgtgcg
     7081 tcggtacggg acaaggcaat ctttctcatg agagcgaatt ccaggatgga ctcattgcgc
     7141 cgctacactt tcggccgagc tcagatcgaa gcgcgccgcg tgactgccga tttcggcgat
     7201 ctctgcatcg gctgtgcccg cgcgagcgat ttcttcgcgt agagcgctcg ccgccccatt
     7261 tgctgcgacc atcagcgagc gtcccgcgtc atcctgcgat tgtccgagaa cttcaaaccc
     7321 atgttcggca caggctgaag caaagtcttc accacccttg ggaaatgtca cgtcgtaaag
     7381 gacaacatcg cgcaccagtg cttcggcgag cggttttgcg atgacaaata tgggcgttcc
     7441 cgccggatga tcagggcgct cgacgaaggg cagccttgcg atgatcttgg gtgcatccgg
     7501 cgcggcaaga ccacgccacc aaggtccggc aagctcatga tgctctgcct gcacgacgcc
     7561 tagatccccg cttgaagccg taacagcctc gataacccct gcagcgcttt gatgcgcgac
     7621 atagggaacc gtgaacccaa aatggaaccg gcaagaatca cgcattgcgg cgtcaccgcc
     7681 cgaaatatcg gtatgaacgc tgtaatttga ttgaacaaag gtgaacgtcg agatgatgat
     7741 cctccagatg ccctcgaccg tatcaagcgg cagcaggcct ttgtggcggg aaacgagtct
     7801 gcgcatcatt tccgcctcgc ggccaggccg gaatgccgag ccgccacctt gccgcgcctt
     7861 ggcttcgatg agccgatgga tgatcttgcc gcgctccatc aacaaaaaat gtagcgccgc
     7921 gtcgattcga tcgatctcac agcgaagctc gtccagagta tcgtgagggg acagatcgac
     7981 catattgcgc cggttccaga tgtgccagaa ctgtcttaga gcattttttg gcgaagtggg
     8041 aagaggctcg gcgtaagcct aggctctata cgccaggctg tttgtgacga gggtcgtaag
     8101 ttcgttatta agtgagcaga cacagctcgg agcaggtttt gtggttgtgt ctgtcccagg
     8161 ccgccgttgc ccttatgcct cttcgtaggc caggcaacag tccagcgacc tagagcaagg
     8221 caaaagagcg caactcatcg cgggaaaatg gccacgggtt ggaaccgcgg ggtaagctcg
     8281 tcggcagggc cgccatcagt cccgcaaaag ttcgttgatg gaggtttttg cccgcgtctt
     8341 cgcatcaacg gttttcacga tcacggcgca gtaaagcgaa gggccgggcg cgccgctagc
     8401 caaaggcttt ccgggcagat tgcctgagac cacgaccgaa taaggcggca catagcccaa
     8461 acggacctcg cccgttgccc tgtcgatgat tttcgtcgag gcagaaataa atgtccccat
     8521 cgcgatcacg gaaccttggc ccacaatcac tccctccacg atttccgagc gcgcgccaat
     8581 aaaacactcg tcttcgatga tggtcggatt ggcctgcaaa ggttcgagca cgccgccgat
     8641 cccaaccccg cccgatagat gcacattttt gccgatctgt gcgcaagagc ctacggtcac
     8701 ccaagtatcg accattgtgt tgctgtcgac ataggcgccg agattgacaa aggacggcat
     8761 caggaccacg cccggcgcga tataggcgga acgccggacg acgcaatttg gcaccgagcg
     8821 gaatcctgcc gcggcatgtt cggcgggccc ccagccggcg agtttggagg gaaccttgtc
     8881 ccaccagctg gtgcccccgg gaccgcccga aatcgcggac atttcgttca agcgaaacga
     8941 caacagcacc gcttttttca gccattcatt gaccctccag gagccgaggc cagaggcgcc
     9001 ttcaatcttt tcggcaacgc ggagcttgcc ggtatcgagc aagctcaagg cagtgtcgac
     9061 ggcatcgcga gtgtcgcctt ttgtcctcgc atcaatgctg gcgcgggtct cgaaagccgc
     9121 attgataatt ctttcgaggt cgagcatcta aatctccaga agcacgtgca tgggtggtga
     9181 ggttttgatg cgccgtgacc agttgtcaat ggcccttaag ggccgctcga attgagacag
     9241 ggcggagcga agccattcag gaattgcgag agatcgcttg tgaaatagtc gatatgaagt
     9301 gccgccgcct cgtgagcagt ctcgaaggct tcgcgatgaa ctttttggcc gggttttggc
     9361 acgaccagca cggtcgtcat ccctttctga tgcggaatga t
//
LOCUS       DATMPU010000089         2172 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_4403890,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000089 DATMPU010000000
VERSION     DATMPU010000089.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 2172)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2172)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2172
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4403890"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(74..629)
                     /locus_tag="VNY06_02045"
                     /pseudo
     CDS             complement(74..629)
                     /locus_tag="VNY06_02045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611663.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(692..1726)
                     /gene="ilvC"
                     /locus_tag="VNY06_02050"
                     /pseudo
     CDS             complement(692..1726)
                     /gene="ilvC"
                     /locus_tag="VNY06_02050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018319725.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
BASE COUNT          446 a          648 c          593 g          485 t
ORIGIN      
        1 cagtatgcgc cataggatga tgaacaaaga actcattatg tccgccatgc gagagctgca
       61 gcgatgcaga cgaggccagg aagttacatg ccaacctgtc atggcgcgtg gcgatacgcc
      121 tgaagtcctt tcaatcgacc gattgtcagt aaaggcgctt gctttggttc cgcgatcctt
      181 cagcccccga cgcaactcgg cgctgtcatc ggccttgtgt cgccgatgag gcgttgcgat
      241 gctgtggcga tgagccgttc cgcggcgggg cagtcatccg ccggtcagga ggatggcgag
      301 aaggtggccc cgagcatccg cgagtgcagg gacctttgtg ttgcgcccgg ctgctttccc
      361 cccttttcca cccgctgccg agcggtgcgc tttgacgtgc ggggagtcga tcatccgggt
      421 gctggccgat ctcccatttc cggccagttc ccggaacaaa ttttcccaga ctccgcgtcg
      481 cgccggcgca ccaatcggtt gcagatcgtc gtcggtgggc cacaggccgg gcaatcccac
      541 cgccgctcaa ccccgcaaac atccgtcggc aagcaaggct caagccgctt ccactgctca
      601 tcgctcagcc aaaacacatt cctgctcaca atttcaattc cttcccgccc aacaatcact
      661 atcttattgt cagcgcagaa agatttatga gctagttctt ttccttgtcc acaagcctgc
      721 ttgcgccgat ccagggcatc atggcgcgca gccttgcacc cacctcttct attgggtgtg
      781 cggccgacct tgcccgcatc gccttaaacg aggtctggtt gacttgattc tcgagcatcc
      841 agtcgcgggt aaagcgtccg gattggatat cgctcaaaac ccgcttcatt tccgccttcg
      901 tctcgggtgt aataatgcgc ggcccggtga catattcgcc atattcggca gtgttcgaga
      961 ccgaataatt catggttgca atgccgccct cataaatgag atcgacaatg agctttacct
     1021 catggaggca ttcaaaatag gccatctctg gcgcatagcc ggcctcgacc aatgtctcaa
     1081 agccgttgcg gatgagttca acgagaccgc cgcagagcac agcctgttcg ccgaaaagat
     1141 cggtttcgca ttcttcgcga aaggtcgtct cgatgaggcc cgcgcggccg ccgccaattg
     1201 ccgacccata tgatagggca aggtcgtgcg cattaccgga agcattgcgg gcgactgcaa
     1261 caagacaagg gactccaccg ccgcgcttat attccgaccg cacggtatgg ccaggccctt
     1321 tcggcgcgac catcaagaca tcgagatcgc cgcgcggctc gatgagattg aaatggatgt
     1381 tgagaccatg cgcgaacatc aaggctgcgc cttctcttaa attgtgacga aggtgctcgt
     1441 gataaatgtc gcgctgcaat tcgtcgggag cgagcatcat caccacatca gcccatttgg
     1501 cggcatcgcc gacagttttt acctcaagcc ccacggcttc cgcctttttg gaggtggcag
     1561 aatccttacg cagggccacg catacctctt tgacgcctga atcccgcaaa ttcaaagcgt
     1621 gggcatggcc ctggctccca tagccgatga tcgcgacttt tctgcccttg ataagattga
     1681 tatcggcatc ggaatcgtaa tagacgcgca taacttgacc ttctctcctt cggcggcgag
     1741 ccttgtgggg gcttcaattg gcatgattta ggtttttagg ccagcccgta atcctttgga
     1801 tcccactcac cggatcaaac gttggcgtag ggcggcggtc cgaacgcaca gcggcaagcg
     1861 gaggcgatag catgaccaac cggttcattg gaattgcagt tttttgactt cttattctgg
     1921 caagcgcctt tgtcgcggag ccatatttgg ttgtctggtg gttctccgcg acggcacctc
     1981 ggactatcac gccgcgctcc gatcttaccg aggcagaacg aacgacggtc aagctatttc
     2041 aagccgtttc cccttcggtc gtatatgtag gacgggactg caggacctct tcacgcctgg
     2101 gcagcaagag agcgaggtgc aggcgggaac gggaatcatt tgggatgccg ccgggcacgt
     2161 gatcaccaac ta
//
LOCUS       DATMPU010000090         3304 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_684840,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000090 DATMPU010000000
VERSION     DATMPU010000090.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 3304)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3304)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3304
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_684840"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..581
                     /locus_tag="VNY06_02055"
     CDS             <1..581
                     /locus_tag="VNY06_02055"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015686491.1"
                     /note="Catalyzes the rate-limiting step in dNTP synthesis;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="vitamin B12-dependent ribonucleotide reductase"
                     /protein_id="HWX13655.1"
                     /translation="SIKYATSILDYVFRELAVSYMERFDLAHVDPSGEGFDALGKGVA
                     EGKPASDTRYVSKGLTRSRTDRLKVMQGGQGVADRASSPSPQGGEGRGEGGPTSKVTA
                     LTSSAPRAEFSGTSALKAEPEQKLSPTELLEQHNRTDARAHARAAAAEKRAEARAKGY
                     EGEACGECGNFTLVRNGTCMKCDTCGSTTGCS"
     gene            790..1152
                     /locus_tag="VNY06_02060"
     CDS             790..1152
                     /locus_tag="VNY06_02060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HWX13656.1"
                     /translation="MANTLTRFGQYCRALRTSHEMTMADQALAFDCPVSLISSIESGR
                     ENPPREYLDQFRKWLNLNDSQHSDLIKRTRSNVVALRQLRSAANNTTSMRLFRKISKM
                     SPEQIRSFRKQVEGEAFE"
     gene            complement(2104..2601)
                     /locus_tag="VNY06_02065"
     CDS             complement(2104..2601)
                     /locus_tag="VNY06_02065"
                     /inference="COORDINATES: protein motif:HMM:NF025559.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-amino-thiazoline-4-carboxylic acid
                     hydrolase"
                     /protein_id="HWX13657.1"
                     /translation="MGVFDDYLVDPKLSLLDKTRIQAQVLVPVLRALRAELGREKADA
                     IVKEALRGWSKNLFAAIADGVDGSPRRKRAAMHCAMAAATEQDATVETRRHDKEALEF
                     DVTHCRFAEFFCALGEAELGALLICETDFDIAAAGGGEVAFTRDQTIMQGAPSCTFRY
                     KFAPR"
     gene            complement(2631..3041)
                     /locus_tag="VNY06_02070"
     CDS             complement(2631..3041)
                     /locus_tag="VNY06_02070"
                     /inference="COORDINATES: protein motif:HMM:NF017470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HWX13658.1"
                     /translation="MANIVGHYLSFEGRLARLPFFSRGVFLGVIAAALAMASIPLFAR
                     EGLGWWFGISVVIASLVLLFVGGFSLTIRRLHDMGLSGYHAIWVSAAQAAWAPLSYGP
                     PEVMLAGLPLAAVGVWLLFWPGNRGPNRFGERPG"
BASE COUNT          741 a         1019 c          940 g          604 t
ORIGIN      
        1 actcgatcaa atacgccacc tcgatcctcg actacgtgtt ccgcgagctg gcggtctcct
       61 acatggagcg cttcgacctc gcccacgtcg acccgagcgg cgaaggtttt gacgccctcg
      121 gcaaaggcgt cgctgaggga aagcccgcgt ccgatacgcg ctacgtgtcg aagggcttga
      181 cccgctcgcg taccgaccgc ctcaaggtga tgcagggcgg ccaaggcgtc gccgaccgcg
      241 catcttctcc ctctccccag gggggagagg ggcggggtga ggggggaccg acttcaaagg
      301 tcactgcgct gacctcctcc gccccccgcg cggaattttc cggcacgtcc gcgctgaagg
      361 cggagcccga gcagaaactc tcaccgaccg agctccttga gcaacacaac cgcaccgacg
      421 cgcgtgccca cgcgcgcgcg gccgccgcgg aaaagcgcgc cgaagcgcgc gccaaaggct
      481 acgaaggcga agcctgcggc gagtgcggga atttcaccct ggtgcgcaac ggcacgtgca
      541 tgaagtgcga cacgtgcggg tcaacgacgg ggtgttcgta aatttacaat cgaggaaaat
      601 ccggccatga ccacgtcacg caaagatgcg tcttgggctt caaggcttct aagaagcaaa
      661 agcaccagca aaaccgtaaa atccgtagca ggttcagact tagcgcaggc tcgccataag
      721 cggcggccgg cccgatcgtt gaataaaaag aaatagttac ttggcagccg cactaactag
      781 gagggggaaa tggcaaacac gctcacccgc ttcggccaat attgccgagc actcagaacg
      841 tcgcacgaga tgacaatggc cgatcaagcc ctggcgttcg attgtccggt aagcctcatt
      901 tcttcgatcg aatccggacg agaaaatcct ccgcgtgagt acttagatca gtttcgcaaa
      961 tggttgaacc tcaatgatag ccaacatagc gacctgatca aacggacccg gagcaacgtc
     1021 gtcgcgcttc gtcaacttcg ctccgccgct aacaatacca cctctatgcg cctcttccga
     1081 aagataagca aaatgagccc tgaacaaatc aggagcttcc ggaaacaagt tgaaggcgag
     1141 gcattcgaat gattgatgac ttttgcgcac ctcctcaatc actggtcaat atagagcaga
     1201 aatcgcttgc gtggcggcac gcgttgggcg ttcatgatac ctgggctcct gacatagcgc
     1261 ggctcatcga gaccaaattg ccaagattga ttccggcttt tgctttagtc gtcggacgaa
     1321 atgagcgccc ttgacaatgc cgaagcttat actgagtttt gacccgccca agatagcagt
     1381 ctctgaaaga gtctacaggc tcgcgtgcca acatgatggt cgagcgcgca tgacgttcgc
     1441 tcacgaattg gggcatcttg ttatgcaccc aggggcagcc aaacctcgaa tcgcaagcgg
     1501 caacaagacc gatcagaaca tcaggatata ccaaagcgct gagtggcagg cgcgcaaatt
     1561 cgccgcctat tttttacttc cgcggaacat cgcgacactg ttttcgtcag ttcaagatct
     1621 agcggagaac tgtcgcgcaa gcttccaagc agctgagata cgattcaaag agcttgggct
     1681 tcccgcccaa cccattcctg actgtgcgaa agaactagcg aagaagattg aggccgcttc
     1741 ttctaagcct tcgttgcgca agatttaacg accgcgctcc gattaaaatt tcgagccaat
     1801 taagcttcta acaacaacag caaattttca cccgatagcg acctaatttt tttcgtcaag
     1861 ctggcaagcg cagagtgggc ataggcgcga agcgccatgc ccacgcgttt tatctgaaaa
     1921 ctcccggatg ttgtgatggg cacgcttcgc tttgcccacc ctacatccgt catcctgagg
     1981 tgcgcgcccc cggcgcgcct cgaaggatgc gagccacact gccacggccg tcgcccttcg
     2041 aggctcgcta cgctcgcgcc tcaggatgac ggaattggct cagcgtaaag cttcccctca
     2101 tctttaccgc ggggcgaact tgtagcggaa cgtgcagctt ggcgcgcctt gcatgatggt
     2161 ctggtcgcgc gtgaaggcga cttcgccgcc gcccgccgcg gcgatgtcga aatcggtctc
     2221 gcagatcagc agggcgccga gctcggcctc gccgagcgcg caaaagaatt cggcgaaccg
     2281 gcaatgcgtg acgtcgaatt cgagcgcttc cttgtcgtgg cggcgtgtct ccaccgtagc
     2341 gtcctgctcg gtcgccgcgg ccatggcgca atgcatcgcc gcccgtttgc ggcgcgggct
     2401 gccgtcgacc ccgtcggcta tggccgcgaa caggtttttc gaccagccgc gcaaggcctc
     2461 tttgacgatc gcatcggcct tctctctccc cagctcggcg cgcagcgccc tgagcaccgg
     2521 caccagaacc tgagcctgga tccgcgtctt gtccaggagc gacagtttgg gatcaaccag
     2581 atagtcgtcg aatacaccca tctcttggtt tcctccggca gggcctgaaa tcaacccggc
     2641 ctttcaccga accggttagg accgcggttg ccgggccaga acaagagcca tacgccgaca
     2701 gcagcaagcg gcagtccggc gagcatgact tcgggcggtc cataggagag cggagcccat
     2761 gccgcttgcg ccgcactaac ccagatcgcg tgatagcccg acaatcccat atcgtgcaga
     2821 cgccgtatgg tcagggaaaa gcctccgacg aagagcagca cgagcgacgc tatgacaacg
     2881 gagatgccga accaccaccc caggccttcg cgagcgaaca agggaatgct cgccatggcg
     2941 agggcggccg cgataacgcc cagaaaaacg ccgcgactga agaacggcag ccgcgcaaga
     3001 cggccctcga aggaaagata atgcccgacg atgtttgcca tggcgcccca ttgtatctgc
     3061 agcgggcgta gctgatcgaa agcaatcgta gcccggatgg agcgaagcgc aatccgagtc
     3121 gatctatccg ctcgccgggc agaaaatttc gtcggtagag gattcatcgt tgaggtttac
     3181 cccggatttc gctttgctcc atccgggcta caattcattg ctaaagctgc gccgccgagg
     3241 cgggcgcgaa gccggcgtgc agccgttcgg acgagcgctc gagctcggct agccgccgcg
     3301 gcag
//
LOCUS       DATMPU010000091         2212 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_1926377,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000091 DATMPU010000000
VERSION     DATMPU010000091.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 2212)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2212)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2212
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1926377"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(251..1239)
                     /locus_tag="VNY06_02075"
                     /pseudo
     CDS             complement(251..1239)
                     /locus_tag="VNY06_02075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018263627.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
     gene            1833..>2212
                     /locus_tag="VNY06_02080"
     CDS             1833..>2212
                     /locus_tag="VNY06_02080"
                     /inference="COORDINATES: protein motif:HMM:TIGR02276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX13659.1"
                     /translation="MHANENGHRGSAILGTVLMLAAMASRVLPARDFAALWALMALVA
                     VFGARPAAAAPFAYVTDSRGSGQSVSVIDTASNTVVAIVPVAANAAGVAANGKHVYVA
                     HILSPGTVSVIDTATNKVVATIPVG"
BASE COUNT          454 a          607 c          669 g          482 t
ORIGIN      
        1 cggaaatggt cgcggctcag aacaacgccc atttcactgg cctttggctc gaagcgccgc
       61 tcggcatggt caaggagcgt gtggccaaac gcgcgatgga tgcctccgat gcaactgtcg
      121 agtttgtcac ggcgcaggcg aaacagcgat cgattggcag cccctcgacg cctcaggctc
      181 tttggaagcg acagtcgcag gtgctttggc cgcgatattg ggatgactgg aaacgatctg
      241 cgcgccccaa ctaccctggc ggaatcagca cctcggcgcc cgtgagcctg cctgctcgca
      301 attggccaag gcgctgtttg cctgattgag cggataagtc ttcgtccgag tctcgatttc
      361 catttgcgcg gcaaaggcga gaaattctat cccgtcctgc cgggtgagat tggcgacgga
      421 aacgatcctg cgctcttgcc acaaccagcg atagggaaaa ggcgggatat cgctcatgtg
      481 tattccggcg caaacaacag tgccgccctt ctgcaccgag gtgagtgcga ccggcaccag
      541 ctcccccagt ggcgcataga tgattgccgc atcgagtggt tctggcggtt tttcgtccga
      601 gctcccggcc cagctggcac cgagccgcaa ggcaaagtcc tgcgccgctt tgtctccggg
      661 gcgcgtgaag gcgaagacat ctcgcccctg tttgcgcgcg gcctgcgcga cgatatgagc
      721 cgccgcgcca aacccataaa ggccgaggac ctttgcgctt cccgcaaaac gaagcgagcg
      781 ccagccgatg agacctgcac ataacaaggg tgcaagcgcg gcggcgctgc tttcttcggt
      841 caatggaaaa gcgtagcgtg cgtcggctat cgcgcttgtc gcatagccgc catttctcgt
      901 gtatccggtg aagatgggcc ggtcgcagag attttctctg tcgctcgtgc aaaaggcgca
      961 ggttccgcac gtcgatccga gccagggaat gccgacccgc gccccgatgg caagcgccgc
     1021 gacgttgggg cctagtgcgt cgatccgccc aacgatttca tgtccaggaa ttatcgggag
     1081 ttttgggtcc ggcaattcgc catcgacaac atgaagatca gtgcggcaga ccccacaggc
     1141 ttcgacgcgg atacgaagtt ctcctggtcc agggataggg tcaggcagct cctcgaggat
     1201 gagaggcttg cctatttcgt gcaggaccat gcccagcatt gtaagcttcc cggagaataa
     1261 tttccgtgcc ccacttgtga atgatggctg cttgcgctta caagcgcact tggccggagg
     1321 tcgtgtgcgg ttttggttgc ttcatcgagc caatctgaag gggcgatctg cgccgttaag
     1381 aaattgggcc gccggatgca aggttttgcc ttaatttagc gggccgtttg gcttttgcaa
     1441 cgaagccggg ttcaagaaaa ccgtgataat tttgcgtgcg tgacgtgtga aaaatcgcac
     1501 caaaatgttt cacgtgaaac attttggtcc gattgcgggc aaaaatctta caaaccagaa
     1561 acagctttgg ctgcggaatt tgtcggatcg ggccgaaaaa cggtctttta ggtcatccgc
     1621 tgcggagctg ctgaatatgc gcatagtgac tcgcgcgcgc cctgccaaaa tttcgaggct
     1681 ttttgaataa atcccacgaa tgatcgatag cgacgttgtg ctcttccgca gaaatggtca
     1741 tggtctagca gtttcgcggt tcggacatct gaagtaagga taagtataag caatcctcaa
     1801 tatttaagag gatttatcta gcggagggga tcatgcacgc aaacgaaaac ggtcatcgag
     1861 gaagtgctat tcttgggacc gtgctgatgc tggcggcaat ggcgagccgc gtcttaccgg
     1921 ctcgggattt tgcggcccta tgggccttaa tggcgctggt tgcggtcttc ggtgcccggc
     1981 cggcggcggc ggcgccgttc gcctatgtga cggattctcg tggttctggt caaagtgttt
     2041 cggtgatcga caccgcaagt aatacggtgg tcgccatagt tccggtggcg gctaacgccg
     2101 ccggggtcgc cgcgaatggg aaacacgtct acgtggcgca tattttgtcc cccggaactg
     2161 tctcggtgat cgatacggcc accaacaaag tcgtggccac gatcccagta gg
//
LOCUS       DATMPU010000092         4004 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_997361,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000092 DATMPU010000000
VERSION     DATMPU010000092.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 4004)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4004)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4004
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_997361"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            423..1382
                     /locus_tag="VNY06_02085"
     CDS             423..1382
                     /locus_tag="VNY06_02085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012383949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase family protein"
                     /protein_id="HWX13660.1"
                     /translation="MKARIREVLSVTTLLLSHPSYALHDTGPHHPERPDRVRAILEAL
                     EAESFAGLHRADAPNIDRAALLRVHPARYIDAIEAAGPAQGTVYLDPDTVMSAKSYQA
                     IAHSAGGAAAAVDAVMTGRANNAFVAMRPPGHHAGMASPMGFCFFNNAAIAARHAKAI
                     YGAERVAILDFDVHHGNGTQEIFWSDPSVLYASSHQMPWFPGTGARAECGEHGNIINA
                     PLSAGDDGAQFREALNSQILPRFENFSPDLIVISAGFDAHRHDPLGGLRLAESDFVWA
                     TTKVMEIAEKHAKGRVVSVLEGGYELEGLARSAAVHVLTLMGA"
     gene            1446..2213
                     /locus_tag="VNY06_02090"
     CDS             1446..2213
                     /locus_tag="VNY06_02090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012384221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HWX13661.1"
                     /translation="MMYRAQPSDGIAWVTGASSGIGRAVALELAQRKFTVAATSRRAS
                     ELESLTEQSSAIFSFPGDITDRAAMATLVSQIESVHGQIALALLNAGIYFISELDRFS
                     ADVVWRTFETNVGGTINCLAPVLAAMERRGKGQIAVTSSLAGYGGIARSSAYGSSKAA
                     LIYMAEALKLTFEPKGLTIQIVNPGFVHTAMTAQNNFKMPFIMVADRAATLICDNFEK
                     GGFEIAFPRRLAYSFKAMCLLPYALYFPLMGRTAKWT"
     gene            complement(2315..3700)
                     /locus_tag="VNY06_02095"
     CDS             complement(2315..3700)
                     /locus_tag="VNY06_02095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012591719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HWX13662.1"
                     /translation="MKARIRGSLDEESRQLLDKMVQNRLNAEPAKAPSSEVCAPQIPE
                     GVMDFQNLPGYRELKLQRSMAALVGLENPYFHLHETAAGATTIIGGRHFTNYSSYDYL
                     GLNHHSDVRTAAQTAIDQFGTSASASRLVAGERPGHQALEKALAQHYCQEDCAVFVSG
                     HATNVSTIGAILGPKDLIVHESLAHNSILAGATLSRAERRSFPHNDAEALDTLLQSIR
                     YQFERVLIVVEGLYSMDGDIPDLPKLVEIKRRHRAWLMVDDAHGLGVLGPKGYGLFDH
                     FGSDPRDVDIWMGTLSKTLAGCGGFIAGPTALVEFLKCFSGGFVYSVGLSPPLAAGAS
                     AALAVLHREPDRVEKLRLISRRFYDSAKARGWNTGTSAGLAIIPFIAGNSIRAVALSQ
                     KLFERGINVQPIIHPAVPERAARLRFFLTSEHRAEQIDETIEAIGDAMNEISESATLQ
                     NIPGFSQGDAP"
     gene            complement(3892..>4004)
                     /locus_tag="VNY06_02100"
     CDS             complement(3892..>4004)
                     /locus_tag="VNY06_02100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013169202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HWX13663.1"
                     /translation="LRDLGISSIRLRTGTPMTYVGLSGFGIEISSTEGLE"
BASE COUNT          873 a         1139 c         1130 g          862 t
ORIGIN      
        1 cccctcgggc aggctacgcg ccattgtgtg ctcccgacac ccgcgcctcg aatttccgtc
       61 ggcagctcca agttagctgg agttttcgtt gttatataga taaactcgcg aaggcatgac
      121 ccagactggg tgagccgaga ctgttacaag gccattgacc cataaccgca tgccgcatta
      181 cccaatccgc ctagtctaaa gcgcgcggtg tgcgtcaccg ccgcatagac agcgggcctg
      241 agacgcaata aaccaggcgc aattccctgc cggaacggac atgggttcta atatcgaacg
      301 tcccctcccc ccgctcggcc gcctatattg gaacgtgccg accggcacgg ggatcggaga
      361 acggactcca tcgaaatggg cagaaattgg cccgcctagt cagtcgtgac accggtgctg
      421 cggtgaaagc aagaattcgc gaagtcctct ccgtgaccac gctcctttta agccatcctt
      481 cctacgccct tcacgatacc ggacctcatc atccagaacg tccggaccgg gtccgggcga
      541 tcctggaggc gctagaagca gagagctttg ctggacttca tcgcgcggat gcccccaata
      601 tagatcgcgc ggcactcttg cgtgttcatc ctgcgcgcta catagacgcg atcgaagccg
      661 cggggccggc tcaagggaca gtctatctcg atcctgatac cgtgatgagc gcaaaaagct
      721 accaggcgat tgcgcatagc gcgggcgggg ccgcagcagc ggttgacgca gtcatgactg
      781 gtcgcgccaa caatgcgttt gtcgcgatgc gaccgccagg ccatcatgct gggatggctt
      841 ccccgatggg attttgcttc tttaataacg ctgccatcgc ggcccggcac gcaaaagcca
      901 tctatggcgc ggagcgcgtg gcgattcttg atttcgatgt acaccatggc aatggaaccc
      961 aggaaatttt ctggtcggac ccgtccgtcc tctacgcctc cagtcaccaa atgccatggt
     1021 ttccgggtac cggcgcccgc gctgagtgcg gcgaacacgg caatatcatc aatgcgccgc
     1081 tctcagccgg cgacgatggt gcacaatttc gcgaggcgct caattcgcaa atcttgccac
     1141 gttttgaaaa cttcagtccc gatctcatcg ttatctcggc tggctttgat gcgcacaggc
     1201 atgatccttt aggaggcctt aggctagccg aaagcgattt tgtctgggcg acaacgaagg
     1261 tgatggagat tgcagagaag cacgcaaaag ggcgagtagt ctcggtgctc gaaggcggct
     1321 atgagctcga gggtctggcc cgatcggcgg cagtgcatgt tttgacattg atgggggctt
     1381 agcttctaga gttgcgttgc gctaagtgcg gccaatgaaa gaaaggagac attgggggat
     1441 cattaatgat gtatcgcgcg cagccatcgg atggcatcgc ctgggtcaca ggagcgagct
     1501 caggcatcgg ccgcgcggtc gcccttgagc ttgcgcaacg caagttcacc gtcgcggcga
     1561 cgtctcgtcg cgcgtcagag cttgagtcct tgacggaaca atcctcggcc atatttagct
     1621 ttcctggcga tatcaccgac cgggcagcca tggccacgct ggtgtcccaa attgaatctg
     1681 tgcatggaca gattgcgctc gccttgctga acgctggcat ttattttatc tcagaactcg
     1741 atcggttctc tgcagacgtt gtttggcgca ctttcgagac caacgtgggc gggaccatca
     1801 attgtcttgc tcccgtgctt gccgccatgg agcggcgcgg taagggtcag attgccgtca
     1861 cgtcatccct tgctggatac ggaggcatcg caaggtcgtc ggcatatggc tcatccaaag
     1921 ccgcattgat ctacatggcc gaagccctca aactgacctt tgagcctaag ggcctcacta
     1981 tacaaatcgt caatccaggt ttcgtccaca cggcgatgac cgctcaaaac aatttcaaaa
     2041 tgccgttcat aatggtcgca gaccgggcgg cgacacttat ttgtgataat tttgaaaaag
     2101 gtggctttga aatcgcgttt ccgaggcggc tcgcctattc ctttaaggca atgtgtctct
     2161 tgccttatgc cttatatttt ccgttgatgg gacgcacggc gaaatggacc tagcgctgaa
     2221 tcgaaagaat cgacgaccgc gccgttgaag cgtctccaga aatgggaact ggtactgtgg
     2281 gacaccttgc gggattctga ctttatcgat acggtcacgg cgcgtcgcct tggctaaacc
     2341 caggtatgtt ttgtaatgtg gcgctttctg aaatctcatt catcgcatcg ccgatcgcct
     2401 cgatcgtttc gtcaatttgt tccgcgcgat gctcggatgt cagaaagaaa cgcaggcgtg
     2461 cggcgcgctc tggaaccgcc gggtggatga tgggctgtac attaattccg cgctcaaaca
     2521 gcttttggct caaagccaca gcccgaatcg aattgcctgc tataaaggga atgatcgcaa
     2581 gtccggcgct tgtccccgtg ttccaaccgc gcgccttagc ggagtcataa aacctgcggc
     2641 tgataaggcg cagcttttcg accctgtccg gttcccgatg cagaacagca agggcggccg
     2701 aagcgcccgc tgccagcggc ggcgaaagcc cgaccgagta gacgaacccg ccggaaaagc
     2761 atttcagaaa ttccacgaga gcggttggac cggcgataaa tccgccgcat ccggcaagtg
     2821 tcttcgacaa cgtccccatc cagatatcga catcgcgcgg atcagaacca aaatggtcga
     2881 ataagccgta tcccttcgga ccaagcacgc ctaacccatg agcgtcgtca accatcaacc
     2941 acgccctatg ccgccgcttt atctcaacga gctttggcag gtcaggaata tcgccgtcca
     3001 tgctgtacag gccttcaacc acgatcagca cgcgctcgaa ctgatagcga attgattgaa
     3061 gcagggtgtc gagcgcttcg gcatcattgt gcgggaacga gcgccgctcg gcgcgggaca
     3121 aggtcgcgcc ggcaaggatg ctattgtgag caaggctttc gtggacgatc aaatccttgg
     3181 gcccaagtat ggctcctatc gtcgaaacat tggttgcatg gccgctgaca aaaacggcgc
     3241 aatcctcctg gcaataatgt tgcgcgagcg ctttttcgag cgcctgatga ccggggcgct
     3301 ccccagcgac aaggcggctc gcagaagccg aagtgccgaa ttgatcaatg gcggtctgcg
     3361 cggcggtgcg cacatcggaa tgatggttga gaccgagata atcataggac gagtaattgg
     3421 tgaaatggcg cccgcctatg atcgtcgtcg cgccagccgc ggtttcgtgc aggtgaaaat
     3481 agggattctc gagaccaacc agagccgcca tcgatcgctg aagcttcagc tcccggtatc
     3541 cagggagatt ctgaaaatcc atcacgcctt ctgggatttg gggggcgcaa acctcagagc
     3601 tcggcgcctt ggccggctcg gcattaagac ggttctgcac catcttgtcg agcaactgtc
     3661 ggctctcttc gtccaaactg cctcgaatgc gtgccttcat gcgaaattct ccaggcggtg
     3721 taagcgctat ctcttatgcg atacgatgct ttatccttcc taccgagttt tggccaagct
     3781 tgaaatgaaa aggaagcgag gaagcacacc aaaaccttgg gttttttggg ccgcagcggc
     3841 agcccggcag gacgttaagg tgaatttggc tgcgggctgg ctcgcgcgag cttactcaag
     3901 cccctcagtt gatgatattt cgatcccaaa acctgacagg cccacatagg tcatgggcgt
     3961 tcccgtgcgc agacggattg atgaaattcc gagatcgcgc agga
//
LOCUS       DATMPU010000093         8762 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_4095177,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000093 DATMPU010000000
VERSION     DATMPU010000093.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 8762)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8762)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8762
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4095177"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1196
                     /locus_tag="VNY06_02105"
     CDS             <1..1196
                     /locus_tag="VNY06_02105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012383462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HWX13664.1"
                     /translation="CGLAQPFVQRRRATRKDRPPISAIIPIKLLDPDFEIAHASMFAQ
                     DYPAYEVLIGAAEQESPALDAAHRIAASHPEVSCRFLRSAATAVSPKLNNLAPPLAAA
                     SHDLVFTKDSNITLDPGTMAAFVQNFTQGVGLVVAVPVAVRAENFAGRIESYLINGHA
                     RIQLTASSLGLGFGIGKTMLFRQSDLTKAGGISALSHALAEDTALSRALARQGLKTVF
                     SHQTVAQEVGRRSFAEIYARQLRWSVIRRQNEPFTFPLEPLASPLPAAFAGALAAPLT
                     SCPSSLAFALTLFTWFCAETGFAFCKGWEVSLWSPLAFLGREILAIVSWLHAFTTHQV
                     VWAQVRFDARHGARGELWRPAAMPDQLGHHLSDTEGCATPRIEEVDKPQHSTGRSGEY
                     KPIGS"
     gene            1258..1749
                     /locus_tag="VNY06_02110"
     CDS             1258..1749
                     /locus_tag="VNY06_02110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012589567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family protein"
                     /protein_id="HWX13665.1"
                     /translation="MFRESFQSHAASLIASCRRTGVMIATAESCTGGLLAGLITTIPG
                     SSEVFDRGFVTYSNAAKIECLGVSPKVLETFGAVSQEIALAMAAGALAHSNADLALSI
                     TGVAGPGGGTLQKPVGLVFFGLARKGCDPKVAEMRFGVIGRDAVRSAAIASAIDLLLE
                     ALR"
     gene            complement(1775..2233)
                     /locus_tag="VNY06_02115"
     CDS             complement(1775..2233)
                     /locus_tag="VNY06_02115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013895635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RatA family
                     toxin"
                     /protein_id="HWX13666.1"
                     /translation="MPSFRTTRRVRHRANEMFDLVADVESYPRFVPLCRGLRVRRRDD
                     ENGKITLIADMEVGYKAIRETFTSRVTCERSEHKILVEYIDGPFKHLENVWNFYDQDE
                     GKSCLVEFRIAYEFRSRALGAIMGGMFDSAFRKFAEAFEDRADAVYGKAR"
     gene            complement(2294..3256)
                     /gene="lipA"
                     /locus_tag="VNY06_02120"
     CDS             complement(2294..3256)
                     /gene="lipA"
                     /locus_tag="VNY06_02120"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018043106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HWX13667.1"
                     /translation="MELSDNLPDQQFRSRHPEKAHRPDQPVARKPAWIRVKAPGSAQW
                     AITHKIVKEHGLVTVCEEAGCPNIGECWEKKHATFMIMGDTCTRACAFCNVKTGLPRA
                     LDPSEPARVAEAVQKLGLAHAVITSVDRDDLDDGGAEHFAKVIAAIRATSPNTTVEVL
                     TPDFLRKQGALEQVVAAKPDVFNHNLETVASKYLKVRPGARYFHSIRLLQRVREIDPQ
                     IFTKSGMMLGIGEERDEVLQLMDDLRTADVDFLTIGQYLQPTNKHHPVLRFVPPDEFA
                     AYAEAALAKGFLYVSASPLTRSSHHAAEDFAWLKARRMALRERA"
     gene            complement(3317..4759)
                     /gene="lpdA"
                     /locus_tag="VNY06_02125"
     CDS             complement(3317..4759)
                     /gene="lpdA"
                     /locus_tag="VNY06_02125"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012254145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HWX13668.1"
                     /translation="MANSYDILIIGGGPGGYVAAIRAAQLGFKTAVVEREHLGGICLN
                     WGCIPTKALLRSSEIFHYLNHANDYGLMVEGRIGFDPDAVVARSRNVSAQLNNGVGFL
                     LKKNKVDIVWGEATIKHAGEVLVTAPRKPTRRPPNPMPKGVLDAGRYRAKNIIIATGA
                     RPRVIPGIEPDGKLIWTYFEAMMPEKFPSSLIVIGSGSIGIEFASFYRTMGTKVTVVE
                     IFPQILPAEDAEIAAHARKNFEKQGIEILTSAKVTKVEKVSDGVVATVEDAQGVARAL
                     EAERVISAVGVTGNVEDLGLEALGVRSEHGIICTDGVGRTNVPGLYAIGDVAGPPMLA
                     HKAEHEGVICVEGIKGLDPHPIDETMIPGCTYCSPQIASVGLTEDKAKALGFEVMVGC
                     FPFLGNGKAIALGESEGLVKTILDKKIGKLLGAHLIGAEVTELIQGFVVAMNCETTEA
                     ELINAVFPHPILSEMMHESVLDAYGRVIHS"
     gene            complement(4794..6128)
                     /locus_tag="VNY06_02130"
     CDS             complement(4794..6128)
                     /locus_tag="VNY06_02130"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007590625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004738 - pyruvate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0004742 - dihydrolipoyllysine-residue
                     acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex dihydrolipoamide
                     acetyltransferase"
                     /protein_id="HWX13669.1"
                     /translation="MPFDILMPALSPTMEKGNVARWLKKEGDKIKAGDVLAEIETDKA
                     TMEVEAVDEGVLAKILVPEGTADVPVNDVIGVIAGEGDDTRSVASRDFQRPSAAGSSD
                     FPKSPQVPSHGQMPAMDRFAPAPASALPPDASQNGQLGERLFASPLARRIAKELGLDL
                     TSIEGSGPHGRITERDVKAARARAPADAPLQETPSAPALPDEAVKKLFAPGSFAEVPH
                     ELMRKTIARRLVEAARTIPHFYLVADCNIDALLALREAINVAAPRDKADTPAYKISVN
                     DFIIKALALAMIRVPDANVTWTEAAMLKHRRADVGVAVAVPGGLATPIVHAAETKSLS
                     TISNEMKELSARARARKLKPEEYQGGSTAVSNLGMFGIKSFSAIINPPQATILAVGAA
                     EQRAIARNSATCVATIMSVTLSCDHRAVDGALGAELLGVFKNFIEKPAGMLA"
     gene            complement(6331..7710)
                     /locus_tag="VNY06_02135"
     CDS             complement(6331..7710)
                     /locus_tag="VNY06_02135"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015666656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex E1 component
                     subunit beta"
                     /protein_id="HWX13670.1"
                     /translation="MVTNVLMPALSPTMEQGKLSKWLKKEGDVVRSGDVLAEIETDKA
                     TMEVEAADDGTLTKILVPDGTDNVAVNTPIAVIAGDGEDAAPVEAPPYLPLRQSGPEV
                     DEAWPPAKAAQAKAALAPAKSSNLEIPEGTAMISQSVREALRDAMAEEMRRDGDVFII
                     GEEVAEYEGAYKVTQGLLQEFGSRRVVDTPITEHGFAGLGIGAAFAGLRPIVEFMTFN
                     FAMQAMDQIINSAAKTRYMSGGQMNCPIVFRGPNGAAARVAAQHSQDYAAWYSHVPGL
                     KVVVPYSAADAKGLLKSAIRDPNPVVFLENEILYGQKFEVPALDDFLVAIGKARVARR
                     GRDVTIVSFGVGMVYASKAADALAGDGIDAEVIDLRTIRPMDSETIIRSVKKTGRCVT
                     VEEGWPQSGVGAEIIARLMEQAFDYLDAPVARICGKDVPMPYAANLEKLALPSVEEVI
                     AAVKTVCYR"
     gene            complement(7753..>8762)
                     /gene="pdhA"
                     /locus_tag="VNY06_02140"
     CDS             complement(7753..>8762)
                     /gene="pdhA"
                     /locus_tag="VNY06_02140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018181590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045250 - cytosolic pyruvate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HWX13671.1"
                     /translation="RKPAVEKTPSAPSNNATFTKEEELSSLRSMLLIRRFEEKAGQMY
                     GMGLIGGFCHLYIGQEAVVTGVRMAAREGDQTIASYRDHGHMLACDMDPKAVMAELTG
                     RRSGAARGKGGSMHMFSREKHFYGGHGIVGAQVPIGSGLAFANRYRSNSNVCFTYFGD
                     GAANQGQVYESFNMAELWKLPVVYIIENNLYAMGTSVSRASALADFSKRGQSFDIPGE
                     QIDGMDVRTVKSAAVRAADWCRSGNGPIILEMQTYRYRGHSMSDPAKYRSKDEVQKMR
                     EERDPIEQVRARVLANKQANEDDLKKIDAEVRAIVAEAADFASHEPEPDSAELWTDVL
                     A"
BASE COUNT         1823 a         2597 c         2386 g         1956 t
ORIGIN      
        1 gctgcggcct ggcgcagcct tttgtgcagc gccggcgcgc gacgcgcaag gatcggccgc
       61 ccatctcggc gattattccg ataaaactgc tcgaccccga tttcgagatc gcccacgcct
      121 cgatgtttgc ccaagactat cccgcctacg aggtcttgat cggcgcagcc gaacaagagt
      181 ctcccgcgct cgacgcggcg caccgcatcg cggcctccca tcccgaggtt tcttgccgtt
      241 ttctgcgctc ggcggccacg gcggtcagcc caaaactcaa taatctcgcg ccgccgctcg
      301 ccgccgcgag ccatgatctc gtattcacca aggattcgaa tatcacgctc gaccccggca
      361 cgatggccgc tttcgtgcaa aattttacgc aaggcgtcgg gctcgttgtc gccgtgccgg
      421 tcgccgtgcg ggccgaaaat ttcgcaggcc gaatcgaaag ttatttgatc aatggtcatg
      481 cccggatcca gctcacggca tcatcattgg gtctaggttt tggtattggc aagacaatgt
      541 tattccggca aagtgatctc actaaagcgg gcgggatatc ggccttaagc catgcacttg
      601 ctgaggatac cgctctctcg cgggcgctcg cgcgccaagg gctaaagacg gttttttcgc
      661 accaaacagt cgcccaagag gtcggcaggc ggagttttgc cgaaatctac gcccggcagc
      721 tccggtggag cgtgatcagg agacaaaatg agcccttcac ctttccgctc gaaccattgg
      781 cgagcccgct tccggcggcc tttgccgggg cgctagcggc gccgctcacc tcctgccctt
      841 cctcgttagc ttttgccttg accttgttca cctggttttg cgccgagacc gggtttgctt
      901 tctgcaaggg ctgggaggtt tctttgtggt ccccgctggc gtttctcggg cgggagattc
      961 tcgcgattgt ttcatggctg catgcgttta caacgcatca ggtcgtgtgg gcgcaagtac
     1021 gcttcgacgc acgccacggt gcccgcggtg aattgtggag acctgcggcc atgccagacc
     1081 agctgggtca tcatctctct gacaccgaag gatgcgctac gccacggata gaggaagtgg
     1141 ataagccgca gcattcaaca gggcgcagcg gcgaatataa accaataggc tcttagaacc
     1201 tgttgagcca caagtggaaa ccggttttgc gtccggacag gcgacgaaag ctcatccatg
     1261 ttccgggaat cgttccaaag tcatgcggcc agcctcattg cttcgtgccg taggaccgga
     1321 gttatgatcg ccaccgccga gtcttgtacc ggcggccttt tggcgggttt gatcacgacc
     1381 ataccagggt cttcagaagt attcgaccga ggcttcgtca cctattccaa tgccgcgaag
     1441 atcgaatgtc ttggggtttc gccaaaggtt ttggaaactt ttggggcggt cagtcaggag
     1501 atcgcgctag ccatggcggc tggtgcgctt gctcattcca atgctgacct tgcgctatca
     1561 attacaggcg tcgctggacc aggaggcggc actttgcaaa agcccgtcgg acttgtgttt
     1621 tttggtctcg cccgcaaagg ctgtgatcct aaggttgcgg aaatgcgttt cggtgtcatt
     1681 ggccgcgacg ccgtccgcag cgccgcgata gcaagtgcaa tcgatctcct tctcgaagct
     1741 ctacgttaac gcaaatgcaa gtaacgcatc cgcctcagcg cgctttgcca tagacggcat
     1801 cggcgcgatc ttcaaaagct tcggcgaact tgcggaatgc tgagtcgaac attccaccca
     1861 tgatagcacc cagcgcacgg ctccggaatt cataggcgat tcgaaattcg actaggcaag
     1921 atttaccttc gtcttggtcg taaaaattcc acacgttttc tagatgctta aacggtccat
     1981 caatgtactc aacgaggatc ttatgctctg agcgctcaca ggtcacgcgg ctcgtaaatg
     2041 tctcgcgaat tgccttatat ccaacctcca tgtccgcaat gagcgttatc ttgccgttct
     2101 cgtcatcgcg tcgtcgcacc cgcaagcctc ggcaaagcgg cacgaatctt ggataggact
     2161 cgacgtccgc cactaggtca aacatttcgt tggcgcgatg gcgaacccgg cgagtcgtac
     2221 gaaaggatgg catgctgctc agaaagcgtg ataagtgaat tgggagcgcc acttgccaaa
     2281 gatttgtaaa tgctcaggca cgctcccgca aggccatccg tcgcgctttt agccaggcaa
     2341 aatcttcagc agcatgatgc gaggaccgcg ttaagggcga ggccgaaacg tacaaaaacc
     2401 ccttggcgag tgcggcctcg gcgtaagcgg caaattcgtc gggaggcaca aagcgcaaga
     2461 ctggatgatg cttgtttgtc ggctgcagat attgccctat cgtcaggaag tcgacgtccg
     2521 cggtgcgcag atcgtccatt agttgcagca cctcatcacg ctcctcgcca atccccagca
     2581 tcattccgga cttagtgaag atttgagggt caatttccct cacgcgctgc aacaatctga
     2641 tcgagtggaa atagcgcgcg cctggccgaa ctttgagata tttggatgca actgtttcaa
     2701 gattatgatt gaacacgtct ggcttggcgg cgacgacttg ctccagcgcg ccttgtttgc
     2761 gcagaaaatc cggggtcaag acttcgacgg tggtattcgg gctcgtcgcg cggatggcgg
     2821 cgatcacttt cgcgaaatgt tccgctccgc catcatcgag atcgtcgcgg tcaacagaag
     2881 taattacggc atgcgcgagc ccgagctttt gaaccgcttc ggcgacgcgt gcaggctccg
     2941 acggatccag tgcgcgcggt agaccggtct tgacattgca aaacgcgcag gcgcgcgtgc
     3001 aagtgtcgcc catgatcatg aaggtcgcgt gtttcttctc ccagcactct ccaatattcg
     3061 gacagcctgc ctcctcgcat acggtcacga gcccatgttc tttaacgatc ttgtgagtta
     3121 tcgcccattg cgccgatcca ggcgccttga cgcggatcca ggcgggcttg cgcgctaccg
     3181 gctggtcggg gcgatgcgcc ttttcaggat ggcgggacct gaattgctgg tccggaaggt
     3241 tatcggaaag ttccatgacg gccattatgt ccaagaatat aggtgttttg caccacaatc
     3301 caaagccgtt gcggcgttac gaatggatca cccttccata agcatcaagc acgctctcat
     3361 gcatcatttc cgatagaatc gggtgtggaa acactgcatt gatgagttcc gcttcggtgg
     3421 tctcgcaatt catggctacg acaaagcctt ggataagttc ggtcacctcg gcgccaatca
     3481 aatgcgcgcc caaaagcttc ccgatttttt tatccaaaat ggtcttaacg aggccctcag
     3541 actctccaag tgcgattgcc ttgccgttgc cgaggaaggg aaagcagcca accatgacct
     3601 cgaaacccag cgctttggct ttgtcttcgg tgaggccgac agacgcgatt tgcgggctgc
     3661 aataagtgca gcccggtatc attgtctcgt ctatagggtg cggatcgagc cccttgatcc
     3721 cctctacgca gatcacgccc tcgtgctccg ccttatgcgc aagcataggc ggtccagcaa
     3781 catcgccgat tgcgtagagt cccggcacat tggtgcgccc cactccgtcc gtgcaaataa
     3841 ttccgtgttc gcttcttacg cccaaagctt caagcccaag gtcctcgaca ttgccggtga
     3901 cgccaacggc ggaaatgacg cgctctgctt ccagagctcg tgcgacgccc tgggcgtcct
     3961 ctaccgttgc cacaacccca tccgagacct tctcgacctt tgttactttg gccgaggtaa
     4021 gaatttcaat gccctgcttt tcgaagttct tacgggcatg tgcagcgatc tccgcatcct
     4081 cggctggtag aatctgcgga aaaatttcaa caaccgtgac ttttgtgccc atcgttcgat
     4141 agaaagaggc gaattcgatg ccgatagaac ctgaaccaat tacgatgagg gagctaggaa
     4201 atttttccgg catcatggcc tcaaaatagg tccagatgag ttttccatcg ggctctatcc
     4261 ctggaatgac gcgaggccgc gcgccggttg cgatgataat attcttggct ctatacctgc
     4321 ctgcgtccaa aacacctttc ggcataggat ttggcggccg ccttgtaggc tttcttgggg
     4381 cggtaaccaa aacctcgcca gcgtgcttga ttgttgcctc tccccagact atgtcgactt
     4441 tgttcttttt gagcagaaag ccgacgccat tattcaattg cgccgaaacg ttgcgggacc
     4501 ttgccacgac agcatcaggg tcaaagccga ttctgccttc gaccatgagg ccgtagtcat
     4561 tggcatgatt gaggtaatgg aagatttctg acgagcgcaa cagcgccttt gttggaatgc
     4621 aaccccaatt gaggcaaatg ccaccgagat gctcacgctc gacaacggcc gtcttgaagc
     4681 cgagctgggc ggcacgaata gccgcgacat agccgccggg gcccccacct atgataagta
     4741 tatcgtagga attagccatt gggcgacaat tcgaaggcag cggggataat gtgtcacgcc
     4801 agcatccctg ccggtttttc gatgaaattt ttgaataccc cgagaagctc agcacctaaa
     4861 gcgccatcca ccgcgcgatg gtcgcaggaa agcgtcacgc tcatgattgt ggcaacgcag
     4921 gtcgcgctat tcctcgcaat cgcccgctgc tcggcggcgc cgacggcaag gatcgtcgcc
     4981 tgcggcggat tgattatcgc cgaaaaactc ttgatgccaa acatgccaag gttcgatacc
     5041 gccgtagagc cgccctgata ttcctccggc ttcagttttc gcgcgcgggc acgagcggaa
     5101 agttccttca tttcgttcga aatcgtcgac aagctcttgg tctcggccgc gtgaacgata
     5161 ggggtggcga gaccgccggg taccgcgacc gcaacgccaa catcggcacg gcgatgcttt
     5221 agcatggcgg cctcggtcca ggtcacattc gcatcgggaa cccgaatcat cgccagcgcc
     5281 agcgccttga tgatgaaatc attgacagag attttgtagg cgggcgtgtc cgccttgtcc
     5341 ctgggcgcgg caacatttat cgcttcccgc aatgccagca gcgcgtcgat attgcagtcg
     5401 gcgacaagat agaaatgcgg aatcgtcctc gccgcctcaa caagccgccg agcgatggtc
     5461 ttgcgcatca attcatgcgg gacttccgca aaggaacccg gcgcgaagag ttttttgacc
     5521 gcctcgtccg gcaaggcggg agccgaggga gtctcttgca gcggtgcgtc agccggagca
     5581 cgcgcgcgtg ctgcctttac atcgcgctcg gtgatgcggc cgtgcggccc ggaaccctca
     5641 attgaggtga ggtcgaggcc caattctttg gcgatacgtc gcgccaaggg cgaggcgaac
     5701 agccgctcgc caagctgccc attctgcgaa gcgtccggcg gcagtgcgct ggctggagcg
     5761 ggcgcgaaac gatccattgc aggcatttgg ccgtgcgatg gaacctgtgg cgacttcggg
     5821 aagtcggagc ttccggcagc ggaaggacgc tgaaagtcgc gcgatgcgac ggacctcgtg
     5881 tcatcccctt cgcccgcaat gaccccaatc acatcgttga cgggaacatc ggcggtgcct
     5941 tccggtacta agattttggc gagcacgccc tcatcgacgg cttcgacctc catagtcgcc
     6001 ttgtcagtct cgatctcggc gagcacatcg ccagccttga tcttatcccc ctccttcttc
     6061 agccagcgcg cgacattgcc tttttccatt gtcggcgaaa gggctggcat gagaatgtcg
     6121 aagggcatag ctatttttct tttccgttca aagccattgg gctcgcgaat gcccaaagac
     6181 attcatgact ttcttgtgtt tgatgggagc aatcaaagga gcagaccaac tttgcacctc
     6241 ccctgcaata atgccaaatt ggcccgagag cacgaccttc ggtgcccgtt cgaataaagc
     6301 tacaacaata tacgcctagc cctagctcgg ctaccggtaa caaactgtct tcacagctgc
     6361 aataacctct tccacgctgg gcagcgccag cttttcgaga ttagccgcgt aaggcattgg
     6421 cacatccttg ccgcaaattc gcgcaaccgg cgcatcaaga tagtcaaaag cctgttccat
     6481 gagcctcgcg ataatctcgg cgccgacgcc gctttgcggc cagccttcct cgaccgtcac
     6541 gcagcgtccg gttttcttga ccgatcgaat aattgtttcg ctatccatcg ggcggatcgt
     6601 gcgaaggtca atgacttcgg cgtcgattcc atcgccggcc agcgcatctg ccgccttgga
     6661 ggcatagacc atgcctaccc cgaaggagac gatggtaaca tccctgcctc gacgtgcgac
     6721 ccgcgccttg ccgatggcca cgagaaaatc atcgagcgcg ggcacttcga atttttgccc
     6781 gtaaaggatc tcattctcga gaaatacgac tgggttcgga tcgcgaatgg cgctcttcaa
     6841 aagtcccttg gcatcggcag cagaataggg cacgacgacc ttgagccctg gaacatgcga
     6901 ataccaggca gcataatcct ggctgtgctg cgcggccacc cgcgcggccg cgccattggg
     6961 gccgcgaaac acaattgggc aattcatctg tccgccggac atatagcgtg ttttggcagc
     7021 ggaattaatg atttggtcca tcgcctgcat cgcgaaattg aaggtcatga attcgacaat
     7081 cgggcgcaaa cctgcgaagg ccgcaccaat ccccagccca gcaaaaccat gttcggtgat
     7141 cggcgtatca accacccggc gggagccaaa ttcctgcagc agcccttgcg tgactttata
     7201 ggcgccttcg tattcggcga cctcctcgcc gataataaaa acatccccat cgcggcgcat
     7261 ttcttcggcc atggcatcgc gcaaggcttc acgcacgctc tgcgagatca tcgccgtgcc
     7321 ttcaggaatt tcgaggttag agctcttggc aggtgcaagt gctgcttttg cctgcgcggc
     7381 ttttgccgga ggccaggctt cgtccacctc cgggccagac tggcgtaaag gaagatatgg
     7441 gggcgcctct accggtgcag catcctcgcc atctccagca atcactgcga tcggcgtatt
     7501 gaccgcaaca ttgtcggtac cgtccggcac cagaattttg gtgagcgtgc cgtcatcggc
     7561 cgcctcgacc tccattgtcg ccttatcggt ctctatttca gcgaggacgt cgcccgaacg
     7621 caccacgtca ccttctttct taagccattt tgagagcttg ccttgttcca tcgttggcga
     7681 aagcgcgggc atgagaacat ttgtcaccat ttggtgtccc agtaatatcc tgccggtctt
     7741 ccgtgcaccc gactatgcta gcacgtccgt ccacaactca gcggagtccg gctccggctc
     7801 gtgcgaggca aaatccgcgg cctcggcaac gatcgcgcgg acctccgcgt caattttctt
     7861 aagatcgtcc tcattggctt gcttgtttgc tagaacgcgc gcgcggacct gctcgatcgg
     7921 atcgcgttcc tcgcgcattt tttgcacttc gtccttcgac cggtatttgg ccgggtccga
     7981 cattgaatgg ccgcgatagc gataagtctg catttcgaga atgattggac cgttgccgga
     8041 cctgcaccaa tcggcagcgc gaacggcggc ggattttacc gtgcgtacat ccatgccatc
     8101 tatttgctcg cctggaatgt cgaaggactg gccacgcttg gaaaagtcgg caagcgccga
     8161 agctctcgaa acagaagtgc ccatggcata gaggttattc tcaatgatat aaacgacagg
     8221 aagcttccaa agttcagcca tgttgaagct ttcataaact tggccctgat tcgccgcgcc
     8281 atcgccaaaa taggtgaagc acacattgct attgctacga taacgattgg caaaggctag
     8341 accgcttccg atcggcactt gcgcacccac gatcccatgg ccgccataaa aatgcttttc
     8401 cctcgaaaac atgtgcatgg agccaccctt gcccctcgcg gcgccgctac gcctgccagt
     8461 caattccgcc atgaccgcct tgggatccat gtcgcaggca agcatatgac catgatcgcg
     8521 gtagctggcg atggtctggt caccctccct cgctgccatc cggacacccg tgacaacagc
     8581 ctcttgacct atgtagagat ggcaaaagcc gccgatcagc cccattccat acatctgccc
     8641 ggccttctct tcgaaacggc gaatgagaag catggatcgt aaggaagaga gctcttcctc
     8701 tttggtgaac gtggcattgt ttgagggcgc gctgggcgtc ttctctaccg caggcttgcg
     8761 gc
//
LOCUS       DATMPU010000094         4723 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_3483675,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000094 DATMPU010000000
VERSION     DATMPU010000094.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 4723)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4723)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4723
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3483675"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(224..601)
                     /locus_tag="VNY06_02145"
     CDS             complement(224..601)
                     /locus_tag="VNY06_02145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX13672.1"
                     /translation="MNKQIAGIFDAAGLMDARGHDLRRTFASVAAELIFSYGDVTVVE
                     LLGHAKRGVTERHYVRHPDAVLISAADRVAHVVAWALGGEQAEVLSLQKEIASQQTRT
                     HRLVSTPRQSTGLVPSLDVHPQS"
     gene            complement(762..1541)
                     /locus_tag="VNY06_02150"
     CDS             complement(762..1541)
                     /locus_tag="VNY06_02150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016920520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HWX13673.1"
                     /translation="MTSARDKIASPGAALVTGAAKGIGRRIAERLAGASYAVVMHCSR
                     GSLADAEAAAANISSNGGKACAMACDLRDTARLSRLTAEAAEIFGPLTLLVNNAALFA
                     ADEAASFELGRFDAHLTVNLKAPLILSRDFAAQLPAGLEAAIVNLIDQRVFRPTPQFF
                     TYSVSKSALWAATRTMAQAFAPMGIRVNAVGPGPVSPNFTQGEAGFALEAHGVPLARA
                     VSPDDVAEAALYLATARNVTGQMIAVDAGQHLAWKTPDVVL"
     gene            complement(1848..3827)
                     /locus_tag="VNY06_02155"
     CDS             complement(1848..3827)
                     /locus_tag="VNY06_02155"
                     /inference="COORDINATES: protein motif:HMM:NF024986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HWX13674.1"
                     /translation="MFRRLREMRLSRARVRARRHAADPAAATPLPTAVPDGAEKFRKQ
                     ARDLDAARKVVEDAAAVSAGLWFSYLSLLLYLAIAVGSVTHKDLLLESPLRLPILNVD
                     LPLVAFFVLAPFMFLVSHAYTLIHFVMLAQKAGALDDEVNAHYGDAPDTREGFRRQLP
                     SNIFVQLLAGPGDIRMGGLGVLLSLIVWITLVLAPVLLLLLIQVQFLPYHNEAVTLWH
                     RAMVLADVILLWALWPAVLESRSTITRPSPKRHPVLALWGLIAIGLAFTAITFPGERL
                     EEWVGSKQWIPPNTVTAWLGARDLDGTAIKTSLHDLLVEGPIDERLRRRKSPFSNTLV
                     LPGFDAPAATKSGDPSKLDSGKPILVLVGRRLEGVDFRNADLRKVELAGVRLQGANLR
                     YAWLQGAWLTEVPLQGASLESAKLQGASLEKAQLSGASLKDAQLQGAWLFKADVRGAS
                     LEQAQLQNTSLKDARLEGTSLKDAKLQGADFRGSKLEGSDLSGAELACANFSDASATT
                     AFRSAAPESKLSPNQADAVSNRITQEVPGGTSIENALKRGEKLKPDAPCTSEIEASRE
                     HALEAAGAVDLQAYQIALADELKSLACSGDSNAAYALRGFITNDRIPDTGKQAARLVD
                     DILKPDCPVSSELTEQDRAALKKLATGVQASESKQ"
     gene            4457..>4723
                     /locus_tag="VNY06_02160"
     CDS             4457..>4723
                     /locus_tag="VNY06_02160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017964538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase"
                     /protein_id="HWX13675.1"
                     /translation="MKSATLKQRSGAAVDKATIEAFRHGFHGQALLSGDAGCDPARRI
                     WNASIDKHPGLIARCSDVADVVRAVKFSRANNLLVAVKSGGHNVA"
BASE COUNT         1001 a         1386 c         1358 g          978 t
ORIGIN      
        1 cgtcattatg tccgccgtcc tgacgcggtg atgatttccg ccgcggaccg tgtggcgcac
       61 gtcgctgcat gggccttggg aggcgagcaa gccgaggtcc tttccctcca ggaagaaatt
      121 gcttcgcgcc gataggtgaa atgagacacg caaggatagg ctggccagca gacaagaacg
      181 catcgcttgg tttccacctc ccttcgatgc ttgcggatgc gcgctatgac tgaggatgaa
      241 cgtccaagct cggaaccaga cccgtactct gacgaggggt ggaaaccaag cgatgcgttc
      301 ttgtctgctg cgaagcaatt tctttctgga gggaaaggac ctcggcttgc tcgcctccca
      361 aggcccatgc aacgacgtgc gccacacggt ccgcggcgga aatcagcacc gcgtcaggat
      421 ggcggacata atgacgctcg gtcaccccgc gtttggcatg tcccagcaac tccacgacgg
      481 tgacatcgcc atagctgaaa atgagttctg ccgcgacgct ggcgaatgtc ctgcgcaaat
      541 catgcccgcg cgcatccatg aggccggctg cgtcaaagat tccggcaatc tgtttattca
      601 aatcagcgct tttgtccgcg tgccggttcg gaaaaaagat ggaatcactg tgattacaat
      661 aggaaagaaa gctatgaaaa agacagggaa aacgtcagaa agttcaatcc tctctcgcga
      721 accattcgtc taatcgaatt ccgtcctggt ataaccgctt cctatagcac tacatccggt
      781 gtcttccagg cgaggtgctg gccagcgtca accgcgatca tctgccccgt cacattccgc
      841 gcagtggcaa gataaagcgc cgcctcggcg acatcgtctg gcgacactgc gcgggcaagc
      901 ggtacaccat gcgcctcgag cgcaaagccc gcctcgccct gggtgaagtt aggcgagacc
      961 ggaccaggcc ccactgcgtt tactcttatt cccatcggcg caaaagcctg cgccatggtt
     1021 cgcgtcgccg cccacagcgc cgatttgctc acactatagg tgaaaaactg tggtgtgggg
     1081 cgaaatacgc gctgatcgat taggttgacg atcgcagctt cgagccccgc tgggagctgc
     1141 gcggcgaaat cgcgtgagag tatcagcggc gctttcaaat tcacggtcag atgcgcgtcg
     1201 aagcgtccga gctcgaaact ggccgcttcg tctgccgcga aaagcgcggc attgttgaca
     1261 agcagggtga gggggccaaa aatctccgct gcttcggcag tgagccggga gaggcgggcc
     1321 gtatcgcgca aatcacaagc catggcgcat gctttgccac cgttcgaaga gatatttgcc
     1381 gccgccgcct ctgcgtcagc gagcgatccc cggctgcaat gcatcacgac ggcatagctg
     1441 gcgccggcaa gacgctcggc aatcctgcgc ccaattcctt tcgccgcacc cgtcacaagc
     1501 gcggcgccgg gcgaagcaat tttatctctt gcgctggtca ttccgttaat tcaggtgagc
     1561 ccctgcgcct catcttatgg ccgggcgaaa ctcggccttc tggcacggca ccagtaacgc
     1621 cggggctgct ccgaacaagt ccgttatgac tcgagagtca tgcgctggac tctggtatat
     1681 ttcggtgccg atcacccacc gccaggcgca ccgatggcat gtcttgaggc gtttgttaag
     1741 ttcgctgggg ccgcgacgtt ggcgttgcgg tacaggtcct cgcgatccgc gtggctctgc
     1801 gcgagcgctc ttgcctgagc gggatcctga aatggcatgc tcataaacta ttgcttgctt
     1861 tcgctggcct gcactccagt agccagcttt ttcagcgcgg ctctgtcttg ttcggtcagt
     1921 tcgctggaga ccgggcaatc aggtttcagg atgtcgtcca ctaatcgggc agcctgcttg
     1981 cctgtgtccg gtatgcggtc attcgttatg aagccgcgaa gggcatatgc ggcattactg
     2041 tcgcccgaac aggcaagact cttgagttcg tccgccaaag ctatttgata cgcttgtaaa
     2101 tcgacggcgc cggctgcttc gagtgcgtgt tcccggctcg cctcgatttc agaagtgcac
     2161 ggtgcgtccg gcttcaattt ctcgccgcgc tttagcgcat tttcgattga tgtgcctccc
     2221 ggcacctctt gcgtaatcct attgctaacg gcgtcagcct gattcggcga gagcttactt
     2281 tctggcgcgg cacttctgaa tgcggtggtc gcggaggcgt ctgagaagtt cgcgcatgcc
     2341 aactccgctc cgctcaaatc ggagccttcc agtttcgatc ctcgaaaatc cgctccttga
     2401 agcttcgcgt ccttgaggga cgtaccttct agccgcgcgt ccttgaggga tgtattctga
     2461 agctgcgcct gttcaagtga cgcgcctcga acgtcagcct tgaaaagcca agcgccctga
     2521 agttgcgcgt ctttaagcga tgcgcctgaa agttgcgcct tctcgagcga ggcgccttgt
     2581 agctttgcac tttcgagaga cgcgccctga agcggcactt cagtaagcca cgcgccctga
     2641 agccacgcat atcgaaggtt ggctccctga agtctcacgc ccgcgagctc aactttgcgt
     2701 aaatccgcat ttctgaaatc aacgccctcg agccgccgtc cgacgagcac gagtatcggc
     2761 ttgccgctgt cgagcttgct tgggtcgccg gatttcgtgg ctgcgggggc atcaaagccc
     2821 ggaagcacca aggtgttcga gaagggactc ttccgccggc ggagccgctc atcgattggg
     2881 ccttcgacca agagatcgtg gagcgaggtc ttgatagcag tgccgtccag gtcccttgcc
     2941 cctagccacg ccgttaccgt gttgggtgga atccactgct tgctgccaac ccactcctcc
     3001 agccgttcgc cgggaaatgt tatggcagtg aaggcaaggc caatggcaat gaggccccaa
     3061 agcgccaaga cagggtgccg ctttggggaa ggccgggtga tcgtactacg gctttccagc
     3121 acggcgggcc acagcgccca caacagaatc acatcggcga gaaccattgc gcgatgccac
     3181 aaagtgacag cttcattgtg gtagggcaaa aactgcacct ggatcagaag cagcaggagc
     3241 accggggcta agacgagcgt gatccacacg attagcgaca agaggactcc gagaccaccc
     3301 attcgaatgt ctcccggccc cgccagaagc tgcacaaaaa tattgcttgg cagttgcctc
     3361 cggaagcctt cgcgcgtgtc gggcgcatcg ccataatgcg cattaacttc gtcatcaagc
     3421 gcacccgcct tctgcgccag catgacgaaa tggataagcg tataggcgtg tgagaccaga
     3481 aacataaaag gcgccagaac aaagaaggcg acgagcggca agtcgacgtt cagaatgggc
     3541 aatcttagcg gggattccag cagaagatct ttgtgggtga cggaaccaac cgcaatagca
     3601 agatagagaa gcagcgagag atagctaaac cacaatcccg cgctgacagc ggccgcgtcc
     3661 tcgaccacct tgcgcgccgc gtcgagatca cgcgcctgct ttctgaattt ttccgccccg
     3721 tcaggaacgg ctgtcgggag gggcgttgca gcggccgggt cagcggcgtg ccttcgcgct
     3781 cgcactcttg cgcgggaaag acgcatttca cgaaggcgcc taaacatttg ggtcctcctc
     3841 aaacccatta ttggcacagg caagcgtgcg cgttaagagc ctatctcaag ggctcacacc
     3901 gatccgcggc ctcctcagca ttccggcaac tggtgaggca tgggtcaaac attgcccccc
     3961 aaaagtttgt ccgattccga gccaaaacct taccaaaatg tttcacgtga aacatttttg
     4021 tccgatcaaa gcccaaaacc ttacaaaccc gcgtacgtct gcaggcttca tctgagtaag
     4081 tttacgcgga aatttggtat aatcggcgag tcgaagctat ggtgatgcgg gtcgcgaggt
     4141 ttggccggat cgatgccgct cacatcaggc gcattgttcc ggcgggtaag accactgttt
     4201 accgccggca attcgtaacc caattttgaa ggcgatgaac cccgtaaacg cttcggtgag
     4261 cgttgcggag ttggcgatac tcgtagtaag ttgagataga gggccgagcg aggttcaccc
     4321 aggccaaaat catcaacatg gagaggtagc aattatcgcg cggtctgtaa cgtgacggcc
     4381 tggcctcgca attaagatgt aatacgcccc acccacgttc ttcccgctgt ctcggtccat
     4441 ttcaacgagg aagaacatga aatctgcaac gctcaaacag cgcagtgggg ccgcggtgga
     4501 caaggcgacc atcgaagcgt tcagacatgg attccatggg caagcgttgc tgtcgggcga
     4561 tgcgggctgc gatccagccc ggcgcatttg gaatgcgagc attgacaagc atccgggcct
     4621 gatcgcccgt tgctcggatg tggccgacgt tgttcgcgcc gtgaagtttt cgcgtgcgaa
     4681 caatcttctc gtcgcggtca aaagtggcgg ccacaatgtc gcc
//
LOCUS       DATMPU010000095         6663 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_3485045,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000095 DATMPU010000000
VERSION     DATMPU010000095.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 6663)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6663)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6663
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3485045"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(312..465)
                     /locus_tag="VNY06_02165"
                     /pseudo
     CDS             complement(312..465)
                     /locus_tag="VNY06_02165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002716583.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3096 domain-containing protein"
     gene            complement(789..1514)
                     /locus_tag="VNY06_02170"
     CDS             complement(789..1514)
                     /locus_tag="VNY06_02170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018267333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HWX13676.1"
                     /translation="MSKNLPDKAMVFAAGLGTRMRPLTDEVPKPLIKVAGKPLIDHAL
                     DRFALAGVATAIVNVHYLASQIEAHVAARKAPKIIISDEREELLGQGGGIAKALPFLW
                     PEPFFIANTDAFWIEGPAESLHQLAAAWNPDEMDILLLVAAVATSIGVDSFGDFNLAP
                     DGRLIRRAERERAPFVYTGVGIIKPELFVGRTVEVFPLTPFFFEAAYRGRLFGQRLDG
                     LWLHVGTPEAIADAEQMIERIRM"
     gene            complement(1751..3307)
                     /gene="tsaE"
                     /locus_tag="VNY06_02175"
     CDS             complement(1751..3307)
                     /gene="tsaE"
                     /locus_tag="VNY06_02175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012589708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HWX13677.1"
                     /translation="MVLDTVQPNASEAVWHRELHDETAIVELAAEVASLAGPGDLMTL
                     SGDLGAGKTAFARALIRLLAGDQNLEVPSPAFTLMQSYEGMSFPILHADFYRIEKPSD
                     LVELGFEEATEGALVLVEWPERADGFLDADRLDIAFRLDEDRGADYRAITMTGTGSFA
                     GRLAHAKAVHELIENSGWHAAKREFLQGDASTRSYERLVKPGGQTAILMYSPQRPDGP
                     PVRYGKSYSAIAKLAENIKPFIAVGRGLRMEGLSAPEIFAFNLEAGLAIIEDLGSEPI
                     TAEGGIIEERYAMALAALAHLHGANLPDTLTLDDAEPYRIPAYDMDALSIEIELLIDW
                     YAPYIARAQLSSGAKATFVNLWRQAVQDILSHRQVWTLRDYHSPNLLWLSAREGLAKA
                     GVLDFQDCVLGHPAYDVVSLLQDARVDILEQEEMKLLAYYARLRREADPSFDVVTFLR
                     AYAILGAQRATKILGVFARLDERDHKPQYLAHMPRVENYLGRDLRHPALAELKHWYEE
                     HLPKIVGVAP"
     gene            complement(3343..5835)
                     /locus_tag="VNY06_02180"
     CDS             complement(3343..5835)
                     /locus_tag="VNY06_02180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012589709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWX13678.1"
                     /translation="MERRSRQQDRTIIWRAGAFAMCLHFVLAASPALGGETGQLGAVA
                     SLRSLAGVAEPTLIAGLAIVLGLATLLHLSQRRRWLETEAGLYSKLSTLQAKLDRAQI
                     FLGAEPQIVVAWGDPGAEPEIQGDAGLVVDGTNPTLVLTFGTWLPSATALTLDTSVSR
                     LRARGEIFRLKATSLDGRYLEIEGRPFGGYAVLRIRGVSGDRLEAARLQSLQAATAEQ
                     LEALRSLLNAVPFPAWTRDGTGKLHWVSAAYAGAVEAKNQEAAVSRGIELIESQAREA
                     SAASRGTGAVWRGRAQAIVAGERRMLEIVDAPAGSGSAGMAADVSELESLRSNLNQQT
                     EAHQRTLDQLSTAVAIFDSAKRLVFCNIAYRQLWSLDQAWLDEKPTDSEVLDRLRAAR
                     LLPEQADFRAWKSELLGAYQSLDTDEQVWFLPDGRTLRAVISPNAQSGVTYLFDDVTE
                     RYRLVSEFNASMRVQSETLDTLKEGVAVFGSDGRLKLFNPAFATLWALRPADLDGQPH
                     IDRVAQLCTPLFADEESWAALRGAVADLPDQRVGFERRLSRSDGSVLDCAAAPLPDGA
                     TLLTFIDMTASVNIERALTERNQALSERNQALTDAERLRNDFVHHVSYELRSPLTNII
                     GFIQLLDDPAIGPLNDKQREYAGYVMKSSSALLALINDILDLASIDADAMELALEEVD
                     IAQIIRAAAGDVEDRLAEASLTLQTAVPENIGGFSADGKRIRQILFNVLTNAVGFSSP
                     GHTIGLAAARKDGKIYFSVTDEGSGIPPEIREHVFDRFKTYTTGSRHRGAGLGLSIVR
                     SFVELHGGSVEIDSVPGEKTTVTCIFPARGRS"
     gene            complement(6065..>6663)
                     /locus_tag="VNY06_02185"
     CDS             complement(6065..>6663)
                     /locus_tag="VNY06_02185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009337927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HWX13679.1"
                     /translation="RLAPQRRRRLSPMNMATELGITTTAIVMLMCMKERTTSIRATKW
                     FTAMKWISLTVHIGATHASAATQNHDMCAAFVHVAADAVVSVLAIIGLLLGRFFGWVF
                     MDPIMGIIGALVIANWAYGLIRDTGGILLDMSADKAIADKLRHMIESGGDLLADLHIW
                     RLGPGHYGAIVSIVTAKSRDVDYYRSCLSRFPVLVCDH"
BASE COUNT         1474 a         1952 c         1877 g         1360 t
ORIGIN      
        1 aatcatatta agccgccttc tgtcgagagc ggccttcctg cggtagaagc gaagtgggct
       61 gcagcgatta tggctacctg tccaagctag ttcgagaggt tcagcggtcc ggttagagcg
      121 atccgatagc gcccacgcca cattcaacat cgtttttcat cagcgactgg aacaacgcgt
      181 cgtgaagaat tgcggctggc gttttgctgt gtgtacgccc gagtcacctt tcggcgagat
      241 tgacaccaat agcctgaact gttgaaggat cgtcagattg cctctttccg tctcctcact
      301 tcaaatttgt atcatcgaca cagcccgagt cccaagatcc cagagacgat caaaaaagat
      361 cgccacgatg taattgagca gccgcggcac gatcaagatg agaatgccgg cgagcgaaga
      421 gacgatcggt cgaaggtgtg cgacgcctat ggtgcctatg gtcattttct tattccctct
      481 actgtaggct tcagttatcg gcggcgaaat cgcgcttgaa aacatggggc ccttcgatca
      541 cttgttgagt tcgccatgat cggagcctaa atgctctaca aaaacttaaa tcgccttcaa
      601 aaccccacga atttgtgcgt catctgttga atgcgggttg tcccatttat tcacttagct
      661 ggcgcgcccc acagatggtg acgagcccgg gcgcgacgac ataaggtagt gcaggtcagg
      721 acatattgac actgctccaa gagcgcaccg ttctcggcct tgatatcgaa ggcgccgctc
      781 acctttccct acatccttat gcgctcgatc atttgttccg cgtccgcaat tgcttcaggc
      841 gtcccgacgt gaagccaaag tccgtcaagc ctttggccaa acaaacgtcc ccgataggcc
      901 gcctcgaaaa agaatggtgt caagggaaaa acttcgactg tcctgcctac gaatagctcc
      961 ggcttgatga tgccgacacc cgtatagaca aagggagccc tctcccgctc tgcccgccgt
     1021 atcaaccggc catcgggagc caaattaaag tcgccgaagg agtcgacacc aatgctcgtt
     1081 gccaccgcag cgacaagcag aagaatgtcc atctcatccg gattccaggc ggcggcgagc
     1141 tggtgcaaac tttcggccgg cccttcgatc cagaaagcgt ccgtattggc gataaaaaac
     1201 ggctccggcc agagaaacgg cagtgccttg gcaattccgc cgccttgacc gagcagctct
     1261 tcgcgctcat ctgagatgat aatttttggg gctttccttg ccgcgacgtg ggcttcgatt
     1321 tgactcgcga gataatgcac attgacgatc gctgtcgcaa cgccagccaa tgcaaaacga
     1381 tcgagcgcgt ggtcgatcag aggctttccc gccaccttga tcaaaggttt gggcacctcg
     1441 tccgtcaagg gtcgcatgcg cgtgcccaaa ccggcggcga aaaccatggc tttgtctggc
     1501 agattcttgg acatgcaagg gccggctaac gaactgttga atgcgatttc tttgtaaggt
     1561 caattctcgg cggcttggca aattttgttt gtgcaactgc gcgcgaagtg cgcgataaaa
     1621 atccaaagga gaccgcatcc gaatatgttg ccggagcaag atgacgtttc ttcgaaccga
     1681 ggtcttgcgt gcgagtctag tttggccgcg cctacagcaa gaccggattt cacctttggc
     1741 tcgacacgtt ctatggagcg acgccaacaa tttttggcag atgctcctcg taccaatgtt
     1801 tgagctcggc caaggcggga tgacggagat cccgtccgag ataattttcg acgcgcggca
     1861 tatgtgcaag atattgcggc ttgtggtcgc gttcatcgag ccttgcaaaa acgcccagga
     1921 ttttggtcgc acgctgcgcg cccaaaatcg cataggcgcg cagaaaagtc acgacgtcga
     1981 aacttgggtc cgcctcacgc cgcagacgcg cgtaataagc gagcagtttc atttcctctt
     2041 gctccaaaat atcgacgcgg gcgtcctgca gaagggagac aacatcgtat gcgggatggc
     2101 ccaagacaca atcttgaaag tcgagcacgc ccgccttggc taacccttcc cgagcggaga
     2161 gccaaaggag atttggggag tggtaatcgc gcaaggtcca aacctgccga tgagacaaaa
     2221 tatcctggac tgcctgccgc cagaggttga caaaagtcgc cttggcaccg gacgaaagtt
     2281 gggcgcgggc aatataaggc gcataccagt cgatcaaaag ttcgatctcg atcgaaagcg
     2341 catccatgtc ataggcagga atccggtaag gctccgcgtc atcgagcgtc aaggtgtctg
     2401 gtaggtttgc gccatggagg tgcgcgagcg cggccaacgc catggcatag cgctcttcga
     2461 tgatgccgcc ttctgctgtg atcggctctg atcccaaatc ttcaatgatt gcaagtccag
     2521 cctcgaggtt aaaggcaaaa atttcaggag ccgagaggcc ttccatgcgc agccctcggc
     2581 cgacagcgat gaatggtttt atattctcgg caagtttggc gatggcgcta taggatttgc
     2641 catatcgaac aggcggtccg tccggacgtt gaggcgaata catcaaaatg gcggtctgac
     2701 cgccgggctt caccaaacgc tcatagcttc gcgtcgacgc atcaccctgc aagaattcgc
     2761 gttttgccgc atgccaaccg ctgttctcga taagttcgtg aacggccttc gcatgcgcga
     2821 gccgcccggc gaaggatcca gttccagtca tagttatggc gcgatagtca gcgccgcggt
     2881 cctcgtcgag cctaaaggcg atatcgagtc tatcggcatc aagaaatccg tctgcccgct
     2941 ccggccattc gacaagaacg agtgcgccct cggttgcttc ctcaaacccc aactcaacga
     3001 gatcgcttgg cttttcaata cggtagaaat cggcatgaag gatggggaaa ctcattccct
     3061 catacgactg catgagggta aatgcagggc tcggcacttc gaggttttgg tcgcctgcaa
     3121 gcagccgaat gagagcgcgc gcaaatgccg tctttccggc accgaggtcg cctgacagtg
     3181 tcatgagatc gccgggaccg gcaaggcttg cgacctccgc ggcgagttcc acaattgcgg
     3241 tctcgtcgtg aagttcacgg tgccagaccg cttcggacgc gtttggttgg accgtgtcta
     3301 agaccatcgc tttagtcccc gctcccgcga cattgcttgg tgtcagcttc gccctcgcgc
     3361 aggaaagatg caagtgacag ttgttttttc ccctggtaca gagtcgattt cgacgctgcc
     3421 gccatgcaat tcgacaaagg accgtacgat ggaaagtcca agtcccgcgc cccggtggcg
     3481 cgaccccgtc gtataggttt taaaccggtc gaaaacatgt tcgcgaatct cgggcggaat
     3541 cccgcttcct tcgtcggtaa cgctaaaata gattttgccg tctttccgcg ccgccgcgag
     3601 gccgatggtg tgccccggcg aggaaaatcc aaccgcattc gtaagaacat tgaagaggat
     3661 ttggcgtata cgtttgccat cggcgctaaa gccgccaata ttttcgggca ccgcagtctg
     3721 cagggtcagc gatgcttcgg ctaggcggtc ctcgacatcg cctgccgcgg cccggatgat
     3781 ttgcgcgatg tcgacctctt ccagggccaa ttccatcgca tcggcatcaa tcgaggcgag
     3841 atctagaata tcgttgataa gggccaggag cgccgacgag gatttcatta cataccccgc
     3901 atattcgcgc tgtttgtcgt tgagagggcc gatcgcggga tcgtcaagca gctgaatgaa
     3961 gccgatgata ttggtgagcg gcgagcgcaa ttcgtaggag acgtggtgga cgaaatcgtt
     4021 gcgcagcctt tccgcgtcag tcagagcctg attgcgctcg ctcagcgcct gatttcgttc
     4081 ggtcaacgcg cgttctatat ttacgctggc ggtcatatcg atgaaggtca gaagggtcgc
     4141 gccatcgggc agcggcgcgg ccgcgcaatc gagcacagaa ccgtcggagc gcgaaagacg
     4201 ccgttcaaac ccaacgcgct ggtcaggcaa gtcggcaacg gcaccgcgca gcgcggccca
     4261 actctcctca tcggcaaaga gcggcgtgca gagctgtgcc acgcgatcaa tatgcggctg
     4321 cccatcgagg tcggccggac gaagcgccca gagggtggca aaggctgggt tgaacaattt
     4381 gaggcgtccg tcgctgccga acaccgcgac gccctctttc aacgtatcga gcgtttcgct
     4441 ctgcacccgc atggaggcgt tgaattccga gaccagacgg tagcgctcgg taacatcatc
     4501 gaaaagatag gtgacgccac tttgcgcgtt ggggctgatc acggcgcgca atgtccgccc
     4561 gtccggcaaa aaccaaacct gttcgtcggt atcgagggat tgataggcac caagcagctc
     4621 cgatttccag gcccggaaat cggcctgctc gggcaacagc cgcgccgccc ggaggcgatc
     4681 gagaacctcg gaatccgtgg gcttttcgtc aagccaggcc tgatcgagcg accaaagctg
     4741 gcgataggcg atattacaaa agacaagacg cttggcactg tcgaaaatgg caaccgccgt
     4801 cgacagctgg tcgagcgtgc gctgatgggc ctccgtctgc tgattgaggt tggaacgcaa
     4861 ggattcgagt tctgaaacat ccgctgccat tccggccgag cctgaaccgg ctggtgcatc
     4921 gacgatttcg agcatccggc gttcgccggc cacaattgcc tgggcgcggc cacgccagac
     4981 cgcgcccgtt ccgcgcgaag cggcacttgc ctcgcgcgcc tgactttcga tgagctcaat
     5041 gccgcgtgac acggcggcct cttgattttt ggcctccacc gcaccggcat aggcagcact
     5101 gacccagtgg agtttacccg taccgtcgcg tgtccatgcc gggaaaggga ccgcattgag
     5161 caaggaacga agggcctcaa gctgctcggc ggtggccgcc tgaagtgatt gaaggcgtgc
     5221 ggcttcaaga cggtcgccgg acacccccct aatccgcaaa acggcatagc ctccgaacgg
     5281 ccgcccctcg atttcaagat atctcccatc gagactcgtc gcctttaaac gaaagatctc
     5341 accgcgcgcc cgcagcctgc tcacggaggt gtcaagtgtc agcgctgttg cggatggcag
     5401 ccacgtccca aaggtgagca cgagggttgg gtttgtccca tccacgacga gcccggcatc
     5461 cccctggatt tcgggctccg cgccaggatc tccccaggca acgacgatct gcggctccgc
     5521 gccaagaaaa atctgagcgc gatcgagctt tgcttgcagt gtggaaagct tggaataaag
     5581 ccctgcctcg gtttcgagcc agcgccgccg ctgggacaaa tggagtagcg tggcaagacc
     5641 caaaacaatt gcgagcccgg caatcagcgt cggctccgcc actccagcaa ggctgcgcaa
     5701 actggccact gcgcccaatt gaccggtctc cccgccaagc gccggcgacg cggccagaac
     5761 gaaatgaagg cacatggcga aggctccagc gcgccaaatg attgtccggt cttgctgccg
     5821 agagcggcgt tccactgatc cctcctcgcg cggctgagcc gggttttatc acccgaatca
     5881 gccaagttta cgcaatcatt cctgccatgt gaaaagtgcc gaacagtgtc cccaatactt
     5941 atatgacaaa tgcgccttgc atcacgattg ccggtactta ccaaggagca gacttgacca
     6001 taaccggaag ttgagcagtc cggccaatgg gctccggttg cttggctccg ccgtcctcgg
     6061 cacttcaatg gtcacatacg agcaccggga aacggctgag gcagctgcgg taatagtcca
     6121 cgtcgcgcga tttcgcggtt acgatcgaga cgatcgcgcc gtagtgtccc ggaccgagcc
     6181 tccagatatg gagatccgcc aacaggtccc cgccgctctc gatcatgtgc cgcagcttgt
     6241 cggcgatcgc cttgtctgcg ctcatatcca agagaatacc gccggtgtca cgaatcaacc
     6301 cataagccca attggcaatg accagggcgc caatgatacc catgatcgga tccataaaga
     6361 cccagccaaa aaaccgccca agaagcaacc cgatgatcgc cagtaccgaa accacggcat
     6421 cggctgccac atgcacgaaa gccgcgcaca tgtcgtggtt ctgcgtggcg gcggacgcgt
     6481 gcgtggcgcc aatgtgcacg gtcaacgaaa tccatttcat ggctgtaaac cacttcgttg
     6541 cccgtatcga tgtggtgcgc tccttcatgc acatgagcat cacaattgcc gtggtggtga
     6601 tgcccaattc cgtggccatg ttcatgggtg ataatcgccg cctccgctga ggagccaagc
     6661 ggt
//
LOCUS       DATMPU010000096         3152 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_2549627,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000096 DATMPU010000000
VERSION     DATMPU010000096.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 3152)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3152)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3152
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2549627"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1361
                     /locus_tag="VNY06_02190"
     CDS             <1..1361
                     /locus_tag="VNY06_02190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018409701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HWX13680.1"
                     /translation="NVRDESAEDIRIVIDPRTRTIDPDIMMEQLFRLTELESRFPLNM
                     NMLADGVVPRVVSFREALLQWLGHRRDVLLRRARHRQAEIMHRLKLVAGMLIVFLNLD
                     VVIRIIREADDPRQELQTRFSLSEVQAAYILDTRLRSLRRLEEMQLRRENDDLAKDKR
                     EIEGLLASEAKQWKTIASEIRALKKLYGPETKIGRRRTSFGQAPDIASADVTETMIER
                     EPVTVVVSEKGWIRALKGQVADLASVQFKGDDALAVSFFAETVSKILVLSSDGRIFTL
                     DAAKLPGGRGQGEPIRLVADIAEGEMIIGVFPYAAGQKMLVASSDGRGFVASQDEMVG
                     GTRKGKLLLNVAKPASSALIVPAEGDHVAVIGENRKLLIFPLVQLPEMSRGKGVRVQR
                     YRDGGISDAKVFFLREGLSWNDSAGRTFSLAARDLRDWVGNRAESGRLPPKGFPKNNR
                     FG"
     gene            complement(1384..2124)
                     /locus_tag="VNY06_02195"
     CDS             complement(1384..2124)
                     /locus_tag="VNY06_02195"
                     /EC_number="2.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012591130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004057 - arginyl-tRNA--protein
                     transferase activity [Evidence IEA];
                     GO_process: GO:0016598 - protein arginylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginyltransferase"
                     /protein_id="HWX13681.1"
                     /translation="MTREPRDAPQFYLTAPSPCPYLPGRQERKVFTHLIGRRATALND
                     QLTQSGFRRSQTIAYRPACERCRACVSIRIKVKEFSFSRNFRRVAEINRDLTGAVVRA
                     EPTSEQYLLFRRYLDARHCDGGMADMTGIDYAMMVEDSHVETRLIEYRLSGQSAGAGE
                     DRPLVAACLTDLLADGLSMVYSFYMPQEQRRSLGTYMILDHIDRARCLGLPHVYLGYW
                     VQGSRKMAYKARFLPQERLGMNGWERVE"
     gene            complement(2169..2642)
                     /locus_tag="VNY06_02200"
     CDS             complement(2169..2642)
                     /locus_tag="VNY06_02200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014890242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HWX13682.1"
                     /translation="MSDREVFDRPAFREPALVEPYLPARALAGVRTRRIVAFFFDLIF
                     VSILCFTLFVALAFLTFGFSLIILPPLFPVVAFFYNGLTVSGHGMATPGMRAMDLEMR
                     LTDGSGVPFLNAAVHAVLFYVSTMFLPVFLVSLFSANKRCLHDMVAGVIVTRRSM"
BASE COUNT          757 a          894 c          880 g          621 t
ORIGIN      
        1 ccaatgtgcg cgacgaatcc gcggaagata tccgtattgt cattgacccc cgcacgcgca
       61 ccatcgatcc cgatatcatg atggaacagc tctttcgatt gaccgagctc gagagccggt
      121 ttccactgaa tatgaacatg ctcgccgacg gtgtggtgcc gcgagtcgtc tctttcagag
      181 aggctttgct gcaatggctc ggacatcgcc gcgacgtgct cctgcgccgt gcgcgccatc
      241 ggcaggccga gattatgcac cggctcaagc tcgtcgccgg catgcttatt gtctttttga
      301 accttgacgt ggtgatcagg attattcgcg aggccgacga tccaaggcaa gagctgcaaa
      361 cgcgtttttc tctttccgaa gtgcaggcgg cctatattct cgacacgcga ttgcgcagtc
      421 tgcggcggct tgaggaaatg caattgcgcc gcgagaacga cgacctcgcc aaagataagc
      481 gcgagatcga gggtttgctc gcaagcgagg ccaagcaatg gaagacgatt gcctctgaga
      541 tccgcgccct gaagaaacta tatggccccg aaacaaagat cggccgccgc cgcacgagct
      601 ttggtcaagc gccagacatt gcgagcgccg atgttacgga aacgatgatt gagcgcgagc
      661 cagttaccgt cgtggtctcg gagaaaggct ggatccgggc gcttaagggc caagttgcgg
      721 atctcgcatc ggtacaattc aagggcgacg acgctctggc cgtctcgttt ttcgccgaaa
      781 ccgtttcgaa aatcttagtg ctttcgagtg acggcaggat tttcacgctt gatgctgcca
      841 aattgccagg cgggcgcggc caaggtgagc cgattcgtct ggtggccgac attgccgaag
      901 gcgaaatgat tattggcgtg tttccctatg ccgcgggcca aaaaatgctg gtcgcttcga
      961 gcgatggccg gggctttgtc gcaagccaag acgagatggt cggcggtacc cgcaaaggaa
     1021 aactgcttct taatgtggca aaaccggctt catccgcgct tattgttcca gccgaaggcg
     1081 accatgtcgc ggtgatcggc gaaaaccgca agctgctcat ctttccgctt gtccaactgc
     1141 cggaaatgtc ccgcggaaag ggcgtgcgag tgcaaagata cagggatggc gggatcagcg
     1201 atgcaaaagt cttctttctc agggagggtt taagctggaa cgattcagcg gggcggacat
     1261 ttagcctcgc tgcgcgagat ttgcgggatt gggtgggaaa cagggcagaa tcgggccgcc
     1321 tgcctccaaa aggatttccc aagaataacc gtttcggctg aagcaaggcg gcgtgcgcac
     1381 cccctactct acccgctccc agccgttcat tccgagccgt tcctgcggca gaaaacgtgc
     1441 cttataagcc attttcctcg agccttgcac ccaataaccg agatagacat ggggcaggcc
     1501 aagacagcgc gcacgatcaa tatggtcgag aatcatatag gttccaagcg aacggcgctg
     1561 ctcttgtggc atgtagaagg aataaaccat cgacagaccg tcagcaagaa ggtcggtcag
     1621 acaggcagca acgaggggcc gatcctcccc tgccccagca ctctgcccgg acaaacgata
     1681 ttcaatgaga cgggtttcga catggctgtc ctcgaccatc atcgcataat caatgccggt
     1741 catatcggcc atgccaccgt cgcaatgccg cgcgtcaagg tagcggcgga aaaggagata
     1801 ttgctcggag gtcggctcag cccgcaccac ggcgccggta aggtcacgat tgatttcagc
     1861 aaccctgcga aaattgcgtg agaaactaaa ctccttgacc ttgatgcgga tcgacacaca
     1921 ggcgcggcaa cgctcacagg ctggccgata tgcgatcgtc tgcgaccgcc gaaatcccga
     1981 ctgcgtgagc tgatcgttca gggccgtagc gcgcctgccg atcaaatggg taaatacctt
     2041 gcgctcttgc cggccaggca aataagggca cggcgacggc gcagtcagat aaaattgtgg
     2101 cgcgtcgcgc ggctctcgcg tcaagctaac tcgccctttc caagttttcg gaacgaaaac
     2161 cctgtttgtt acatgctcct ccgggtgaca ataacgccgg caaccatatc atgcaggcat
     2221 ctcttgtttg cgctgaaaag cgaaacgaga aacaccggca ggaacatggt actcacataa
     2281 aagagcacag catgcactgc ggcgttaaga aaaggcaccc cactgccgtc cgtaagacgc
     2341 atttcaaggt ccatggcccg catccccggc gtcgccattc cgtggcctga gaccgtgagg
     2401 ccattataga aaaatgccac gactggaaag aggggcggca agataataag cgaaaacccg
     2461 aaggtcaaaa aggcgagggc aacgaagagc gtgaaacaaa gaatggaaac gaaaatcaaa
     2521 tcgaaaaaaa atgccacaat ccgccttgtg cgaacgcccg caagagcgcg cgcgggaaga
     2581 taaggctcaa caagcgccgg ctctcgaaag gccggtcgat caaacacctc cctgtcgctc
     2641 attatcatat ccctcgggcg aatcttctca aataaccaac tttacctgcc tccgagaccg
     2701 gagcgcgata cgctgcggtc tccaatgtgg tcctaatgac aacatggcac aagtgccgcg
     2761 gtccaaattg aaatcttcac ctgtcgggcc gcgccaaatt ctgttcttgc catgttacgc
     2821 gcccacgcca aaaagggaag atgaagcgtc cgcccttttg ttttttcgtt tagcttggcc
     2881 ttttcagcct agattcgggg cggcgtttcg ctcctgcagc acgcaaatgc gcaggctcgg
     2941 ccatccctca ttggccagga cagcttcaag attctaccaa cgcctgcgca tgcagcaatc
     3001 tgaaactgcg agagacgaag ctgttcaatc gagagctacg tccacgagtg acatgttgca
     3061 gcgcagttgc gcgtaacagt cattgattgc atgccgcctc caggcctacg atccgggcag
     3121 aacaacccgc cccgcaatgg cattcgcggc tg
//
LOCUS       DATMPU010000097         3987 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_3174902,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000097 DATMPU010000000
VERSION     DATMPU010000097.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 3987)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3987)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3987
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3174902"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..129)
                     /locus_tag="VNY06_02205"
     CDS             complement(<1..129)
                     /locus_tag="VNY06_02205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006023409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HWX13683.1"
                     /translation="MQGVERHVTLITGASGGIGADLARVFARHGHDLALVARRGAKL"
     gene            complement(189..785)
                     /locus_tag="VNY06_02210"
     CDS             complement(189..785)
                     /locus_tag="VNY06_02210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020174074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerB family tellurite resistance protein"
                     /protein_id="HWX13684.1"
                     /translation="MSVVALNGKMRFPPAGTIMRSGPKSEALMFDALKRFVEEVSGAQ
                     VPRRNFGEEDYRLAAVALLVHVANVDGKVDLAERRELQTIIKERFGLDTTATAELTAL
                     AEQSDREAVDFYHFTSVLKRALDDGERLKIVEMLWNIAFADGAVHELEENTIWRIAEL
                     LGVSTRDRVKLRQKVAGQPPSDPYAGSPWSVSNEEGKS"
     gene            1412..3847
                     /gene="pheT"
                     /locus_tag="VNY06_02215"
     CDS             1412..3847
                     /gene="pheT"
                     /locus_tag="VNY06_02215"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019904746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HWX13685.1"
                     /translation="MKFTLSWLKDHLETDATLEEIAATLTKIGLEVEHVDNKASALKG
                     FVIARVVEAKPHPNADRLRLCFVDVGEGPPVQVICGAPNARTGMKSVFAAPGTYIPGK
                     KIMLGKGVIRGIESNGMLCSGAELELSDEYDGILDLPDAAPVGAAYAAWALLDDPVIE
                     INLLPNRPDAAGVNGIARDLAAAGLGRLKTAPITPVNGNFPCPVAVRLDFPPQDAHLA
                     PLFALRLVKGVRNRQSPEWLQRRLKAIGLRPINALVDITNFLTYDRARPLHVFDAQKV
                     KGNLIVRRAKPGEELIALDGRTYKLHGENVVISDATGVESLAGIMGGQATGCDETTSE
                     VLIESALWDPVNIARSGRWLGILSDARYRFERGVDPVFCLPGLELATNLVIEFCGGVA
                     SGTCVAGTIAPPDRRMTFPWGELNRLTGLSLEISEMERILESLGFELGNSSENPGEVS
                     VKVPSFRPDIEGKTDLVEEILRIAGVDRVAPAALPRAFGDHVKGSVLTPLQRRIRITK
                     RALASNGLVEAVTWSFVSRQRALAFGGGGASLALANPIAPEHSDMRPSLLPGLIAAAQ
                     RNADRGFGDVGLFEVGQIFLSDSEEGEKIAATAIRRGMAKEKGIGRHWSMPHATVDSF
                     DTKADALALLSALGVPAAGLQLIPGGPPWFHPFRSATIQLGPKNVIGAFGELHPRTLE
                     AVGAEEPVAGFELFLNDIPAPKLRSTKAKPKLELSEFMPVQRDFAFLADQSVKAADIV
                     KAAAAAERALVAHVGVFDVYEGAGIPEGKKSVAAFVTLQPREKTLTEAEIDAAAGKII
                     AEVKKKTGASLRG"
BASE COUNT          824 a         1174 c         1163 g          826 t
ORIGIN      
        1 gagtttagcg cccctgcgtg cgacgagagc gagatcatga ccatgacggg cgaagacgcg
       61 ggcaagatcg gccccgatcc cgcccgaggc gccggtaatc agcgtgacgt ggcgctcaac
      121 accctggatc cgggcttctt ccgggcccgg cccgacctcg acggagggct caatgcttac
      181 ggcttcgctc acgatttccc ttcctcgttc gacactgacc aagggctacc ggcgtaaggg
      241 tccgaaggtg gttgtccagc aaccttctga cgcaacttaa ctcggtctcg cgtagatacg
      301 ccgagcagtt cggcaatccg ccagattgtg ttttcttcca actcatggac tgcgccatcc
      361 gcaaacgcaa tgttccagag catctccaca attttcagcc gctcgccgtc atcaagcgcg
      421 cgtttgagaa cgcttgtgaa atggtagaaa tcgaccgctt cgcggtcgct ttgctcggca
      481 agggcggtca gctcggcggt ggccgttgtg tcgaggccga accgctcttt gatgatcgtc
      541 tgcagctcgc gccgctcggc gagatcaact tttccgtcca cattggcgac atgaaccagc
      601 aaagccacag cagcgagacg gtaatcctcc tcaccaaaat tccggcgagg gacctgtgcg
      661 ccggaaacct cttcgacaaa gcgttttagg gcatcgaaca tcaaagcctc cgacttaggt
      721 cccgaacgca tgatagtgcc cgcgggcgga aagcgcattt tgccgttcag cgcaacaaca
      781 gacaagtcca gtaaccctcg ggccggtgat aagcgcggcg taaccggccg atccagtcct
      841 ggcatgggtg acgagaggga aaacagactt tctcgtcaag ccaatcgatg agagtcaagc
      901 cagttacatc cttatagggg cggccgccgg aaaaatgccc gagccaaacg gatcgccaag
      961 gcaattcctc ggccgttgct gcaggtggtg cccaaaatgg cgccccgacc gagcagggaa
     1021 tgcccccata atctgtaact tgtccgattg cagggtgtgt atcccaatcg gcagcgcggc
     1081 ttacgccaca aaaggccaaa catagtgcgc ccaagacgat tcctgcggtt atccgcctag
     1141 ccgcacgcca tgcggccccc gttttcctcg cgttcacaac tacaaattta cctagcttta
     1201 ccgtaacaat acacatggca ccctcttcta tcctggataa ccgccaggct caacctgtta
     1261 tcaagcgggc aatctgccat agcgtcgggc caatagctgg ccgggcggga taggctgctg
     1321 ctcatttgcc agagatgaga gcgaagaaag atttccctca cggccaaaac acactagaga
     1381 cacaaattct ctggcacctt ttggaaccga aatgaagttt accctatcct ggctcaagga
     1441 tcatctcgag accgatgcga cgctcgaaga gattgccgcg acgctgacca aaattggtct
     1501 tgaggtcgag cacgtcgata acaaggcttc cgcgctcaag ggctttgtga tcgctcgcgt
     1561 tgtcgaggcc aagcctcacc ccaatgctga ccgtctgcgg ctttgctttg tcgatgtggg
     1621 agaaggcccg ccggtgcagg ttatttgcgg cgcgcccaat gcccgcaccg gcatgaagtc
     1681 ggtgtttgcc gcgccaggga cctatattcc cgggaagaag atcatgctcg gaaagggcgt
     1741 cattcgcggc attgagtcaa acggcatgct ctgttcgggc gccgaacttg aattgtccga
     1801 tgaatacgac ggcattctcg atttgccgga tgcggctcca gtcggcgcgg cctacgctgc
     1861 atgggcattg ctcgacgatc cggtgatcga gattaatctt ttgcccaacc ggccggatgc
     1921 tgcgggggtc aacggtatcg ctcgcgacct cgcagcggcg ggcttgggca ggctgaagac
     1981 cgcaccaata acgcccgtga acggcaattt tccctgccca gtcgcggtgc ggctcgactt
     2041 cccgccgcag gacgcgcatc tcgcgcctct gtttgcattg cgtctcgtga aaggcgtgcg
     2101 caatcggcaa agccctgagt ggctacagag gcggttgaag gcaatcggtc ttcggccaat
     2161 taacgcgctc gtcgatatca cgaactttct tacctacgac cgggcgcgtc cgctgcatgt
     2221 tttcgatgcg caaaaagtca agggcaatct catcgttcgc cgcgccaagc cgggcgaaga
     2281 gctaatcgcg cttgacggcc gtacttataa gctccacggc gaaaacgtcg tgatctccga
     2341 tgcgacggga gtcgaatccc ttgctgggat catgggcgga caggccactg gctgcgacga
     2401 gacgacgtcc gaggtgctga tcgaatcggc cttatgggat ccggtaaata tcgcgcggag
     2461 cggacgctgg ctcggcattc tttccgacgc gcgctatcgc ttcgaacgcg gcgttgaccc
     2521 cgtattctgt cttcccggac ttgaacttgc gacgaatctg gtgatcgaat tttgcggcgg
     2581 ggttgcctca gggacatgtg tcgcggggac aatagctccg cccgataggc gtatgacttt
     2641 tccctggggc gaactgaatc gcctgacggg tctttcgctc gagatctcgg agatggaacg
     2701 gatactggag agccttgggt tcgagcttgg caattcttcc gaaaatccgg gggaggtcag
     2761 cgtaaaagtg ccctctttcc gccccgatat cgagggcaag accgaccttg tcgaggagat
     2821 cctgcgtatt gcgggagtcg atcgggttgc gccggcggca ctgccgcgcg cgttcgggga
     2881 ccacgtcaag ggatccgtgc tgacgccgct gcagaggcgt attaggatca ccaaacgtgc
     2941 cctcgcctcc aatggcctcg ttgaagccgt cacctggtcc ttcgtttcgc gtcagcgggc
     3001 actcgcgttt ggcggtggtg gggcatctct tgctctagcc aatcccattg cccccgagca
     3061 ttccgatatg cgcccgagcc tgcttccggg tttgatcgcg gcggcacagc gcaacgccga
     3121 tcgcgggttt ggcgatgtcg ggctgttcga agtcgggcaa attttcttga gcgacagcga
     3181 agagggcgag aagattgcag cgaccgcgat tcgccgcgga atggctaagg aaaaaggaat
     3241 tggacggcat tggtcgatgc cgcacgccac ggttgattcc ttcgatacca aggcggacgc
     3301 tctcgctctt ctttctgcgc tcggcgtgcc ggccgccggt ctgcaactca ttcccggcgg
     3361 acctccatgg ttccatccct tccggtcggc cacgatccaa ttggggccaa aaaatgtcat
     3421 aggcgcgttt ggggaactcc atccacgcac cctcgaagcc gtgggagcag aggagccggt
     3481 cgctggtttt gagctgtttc tcaatgacat accggcgccg aaattgcggt ccacaaaggc
     3541 caaaccgaaa ctcgaacttt cggagtttat gcccgtgcag cgtgacttcg ctttcttggc
     3601 tgatcaaagc gttaaagcgg ccgatatcgt gaaggccgct gccgcggctg agcgcgcgct
     3661 cgtcgctcat gtcggcgtct tcgatgtcta tgaaggcgcg ggaatacccg aagggaagaa
     3721 atcggttgcc gctttcgtta ccttgcaacc gcgtgaaaaa accctgacag aggccgagat
     3781 tgacgccgca gctggcaaga tcatcgccga agttaaaaag aaaactggcg cgagcctcag
     3841 aggatagaac cttgagaacc attctgcttg cctttttcac ggccgcgttg attgtggttt
     3901 cgggtcaatc aaaagccgct ccgcgcaaca tcgacgagtg cgaaaaaatt caggcggcgg
     3961 atgcttacaa tctctgcctg gcctcat
//
LOCUS       DATMPU010000098         7550 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_2550452,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000098 DATMPU010000000
VERSION     DATMPU010000098.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 7550)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7550)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7550
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2550452"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..671)
                     /locus_tag="VNY06_02220"
     CDS             complement(<1..671)
                     /locus_tag="VNY06_02220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009859278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HWX13686.1"
                     /translation="MKFPQAEDIREKLEKRLGRLHARQRLGIEKDHEGQVFGQGMRFL
                     HIENLLFSRSLIEGVLRLSGLYWQGRRNACKIRVKHNDIVSRRVPRPFDGFRILHLSD
                     LHTDMSEPAMSRILDLLPGLEYDLCVLTGDYRGKTFGSFDLALARMARLREALNGPVF
                     GVLGNHDTIQMVPGLEGMGIRMLLNESVGIERGGQRIHLAGIDDADFYKVDNIEKAAD
                     TIPHE"
     gene            1286..2584
                     /gene="glyA"
                     /locus_tag="VNY06_02225"
     CDS             1286..2584
                     /gene="glyA"
                     /locus_tag="VNY06_02225"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014889958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HWX13687.1"
                     /translation="MSQTAPSPTIQYPFFSAGLAVADPEIAKAIELELGRQQHEIELI
                     ASENIVSKAVLEAQGSVMTNKYAEGYPGRRYYGGCQFVDMAENLAIERVTRLFDCKFA
                     NVQPNSGSQANQGLFLALMQPGDAFMGLDLAAGGHLTHGSPVNLSGRWFKVVPYGVRK
                     DDYRIDMEALARLAEEHNPKLIIAGGSGYPRHWDFAGFRAIADSVGAYFVVDMAHFAG
                     LVAGGVHPSPFPHAHVVTSTTHKTLRGPRGGLILTNDEAIAKKLNSAVFPGLQGGPLM
                     HVIAAKAVAFGEALKPEFKIYAKQVADNAKALAASLTERGYAITTGGTDNHLMLVDLR
                     PKQLTGKAAEASLGRAGITCNKNGVPFDTASPMVTSGIRLGTPAATSRGFGIAEFKKV
                     GALIAETLDGLAKNGDAGNAGVEAKVKADAAALTERFPVY"
     gene            2679..3197
                     /gene="nrdR"
                     /locus_tag="VNY06_02230"
     CDS             2679..3197
                     /gene="nrdR"
                     /locus_tag="VNY06_02230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014889957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HWX13688.1"
                     /translation="MRCPYCGSLETQVKDSRPSEDSSAIRRRRACPDCGGRFTTFERV
                     QLRELMVVKKSGRRVPFDRDKLMRSLEIALRKRPVDPERVERMVNGIVRQLESQGDSD
                     IPSERIGELVMEGLRALDSVAYVRFASVYRNFREARDFNALIDELANGADGTPDQPTT
                     PSPTKARSPSQA"
     gene            3194..4306
                     /gene="ribD"
                     /locus_tag="VNY06_02235"
     CDS             3194..4306
                     /gene="ribD"
                     /locus_tag="VNY06_02235"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012384225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HWX13689.1"
                     /translation="MSQSNEDLRFMAAALAIGRRGLGLSAPNPSVGALVVKDGVILAR
                     GWTKPGGRPHAETEALRDASLDATGATLYVTLEPCSHHGQTPPCTDAIVASGVSRVVY
                     AIDDPDPRACGSAEKILETAGIGVTKGVLEVEAKHANLGHFLCVTQKRPMITIKLVLT
                     ADFYAARQGGEPRLLITGEPANGLVHIMRAMHDAIMIGIGTVLADDPLLTVRLPGVED
                     RKPMRIVLDGDLRLPPQSRLAATAAKIPALAIAGEGASEDRAARLRVAHVEIAHVRRD
                     AAGHVDLEAALNLLASRGLTRIFCEGGPRVAAALIGQRLADEVFLFTAPKPLAQKGVL
                     GLDAATASVLADPQQYLLAESRMIGADRLTRRERVD"
     gene            4307..4930
                     /locus_tag="VNY06_02240"
     CDS             4307..4930
                     /locus_tag="VNY06_02240"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015666435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HWX13690.1"
                     /translation="MFTGVVSDVGVVEEAEARANLRRLHIACAYPAERVTPGASIACA
                     GVCLTVTASGSSGKGGWFEVDAGLETLARTTAKHWKKGTRLNLERALRLGDELGGHMV
                     SGHVDGVAEIVSIEEFEGMRRIGFEAPHDLARFIAEKGSVALDGTSLTVNWVEGDIFS
                     VLLIPHTLKVTTWESAQISMGVNLEVDLLARYAARLTEARGGQQQGA"
     gene            5072..5752
                     /locus_tag="VNY06_02245"
     CDS             5072..5752
                     /locus_tag="VNY06_02245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX13691.1"
                     /translation="MQTGQELLQKWDARAPRLYLRMEVTGEGLALGARTILAKMARDE
                     RGAPFLFLEDEQRTIALLTTAYQRPFEPYLLAKLSRACELWSENEKALAHIHLTHAGL
                     PPCDEGQALRLFVAEELLESETTPHTLLKAQGFDTAALDLSKFSPAQPRVSSGNGRES
                     GQWTSGDADISPVAFRSPKERRGHRGGGGVFDAIRSFFGPATRRAKAERKRGCDGEKT
                     GSGRRLQT"
     gene            6103..6384
                     /locus_tag="VNY06_02250"
     CDS             6103..6384
                     /locus_tag="VNY06_02250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX13692.1"
                     /translation="MNNEKDSDDYKRTQEIRAILLKDWDPLGIGDNPNLADEYDHYIP
                     AILRLFDTHGTVEELERYLADIEEKWELTPDPAAWRAAKKIFDALKESE"
     gene            6626..6865
                     /locus_tag="VNY06_02255"
     CDS             6626..6865
                     /locus_tag="VNY06_02255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX13693.1"
                     /translation="MPPCELVAAVVAPPTLLKAQGFHPPPLDMLKFNPAQPRVPSGNG
                     RESGQWTSGDADITPVAFEGDTATAVEAAYLMRFF"
     gene            7315..>7550
                     /locus_tag="VNY06_02260"
     CDS             7315..>7550
                     /locus_tag="VNY06_02260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX13694.1"
                     /translation="MSDEDRAQRVRDTLLKDWDPLIVGDNPHLTDEYDDLIPGILHLL
                     DAHCTAEQLGHHLASIDAKWGGEPAWPNIERTVG"
BASE COUNT         1644 a         2137 c         2227 g         1542 t
ORIGIN      
        1 tcttcgtgcg ggatcgtgtc ggcagctttt tcgatattgt ccactttata aaaatccgcg
       61 tcatctatcc ctgccagatg aatgcgttgc ccgccgcgtt cgatgccaac gctttcgttg
      121 agcagcattc gtatgcccat cccctccaga ccgggtacca tttggatcgt atcgtggttg
      181 cctaagacgc cgaacaccgg accattaagc gcctcgcgga gccgagccat tctagcaagc
      241 gccagatcaa acgatccaaa agtcttcccc ctatagtcgc ctgtgaggac gcaaaggtcg
      301 tattctaggc ctggcagaag atccaggata cggctcattg ccggctcgct catgtccgta
      361 tgcagatcgc tcagatgcaa tatcctaaag ccgtcgaatg gcctcggaac gcggcgcgag
      421 actatgtcgt tgtgcttaac tcggatcttg caggcattcc gccgtccctg ccaatacaat
      481 ccggatagcc gaagaactcc ctcgattaga ctgcgcgaga aaagcagatt ctcgatatgg
      541 agaaagcgca taccctgtcc gaagacctgc ccctcatgat ccttctcaat gccgaggcgc
      601 tgccgcgcgt gcagccggcc gagccgcttt tctaatttct ccctaatgtc ttccgcttgg
      661 ggaaacttca ccggggcatg taatggatgc ttcattcgca ggtcacagat ggcccgaggc
      721 tcattggctt gccatgcttc ttaactcctt taggatacca gacaagattg acctggagct
      781 gcccgcatag ggagagacgc gagactggtt ggattgaagc ttgtctcagg gcaggggcac
      841 ccatctaaaa aatgaaaaat tggcacgttt tgagtggtgg tcgcttagtg ccaccgcaat
      901 aggcaaatag aggcgttggc aattaacccc tgccagctgg gcagagatgt gtcccggatc
      961 tcccagagcg gccccggact ctgaggactt gctaaatcgc cgcccaactt gccggtattt
     1021 cgcctaatcc cgcccgccaa aggccgtcag acgttatgac cgtgtaagat ttttgcccat
     1081 tatcagacaa aaatgatttc acgcgagaca tttttgaaga tttagaaacg gaatcggacc
     1141 agttttgcgc accaaatttc gcccgcgaaa gccgggctcg cgccttcacg cgggcaaaag
     1201 ggcgtgctaa caaaaaaccc ttaatcaccg tgcctggctg gcagcattgc cgttgccgct
     1261 aggtttttag agcaggatcc gcagcatgag ccagaccgct cccagcccaa caatccaata
     1321 tccctttttt tccgctgggc ttgccgttgc cgatcccgag atcgccaagg cgattgagct
     1381 cgaactcggc cgtcagcagc atgaaatcga gctgatcgcc tcggaaaata tcgtctctaa
     1441 agccgtgctc gaagcccagg gttcggtgat gacgaacaaa tatgccgagg gttatccggg
     1501 ccggcgctat tatggcggct gccaatttgt cgatatggcc gaaaacctcg cgatcgaacg
     1561 tgtgacccgg ctctttgact gcaaattcgc caacgttcag ccaaattcag gcagccaggc
     1621 aaaccagggc ctatttctgg ccctgatgca gccgggcgac gcgtttatgg gccttgatct
     1681 cgcggcgggc ggccatctca cccatggctc gccggtcaat ctctccgggc gctggttcaa
     1741 ggtggttccc tatggggtcc ggaaggacga ctatcggatc gacatggagg cgctcgcccg
     1801 cctcgccgag gagcataacc ccaaacttat catcgccggc ggctcgggct acccgcgcca
     1861 ttgggatttt gccgggttcc gagcgatcgc ggattcggtt ggcgcttatt tcgtggtcga
     1921 tatggcgcat ttcgccgggc ttgttgcggg cggcgtgcat ccctcgcctt tcccgcatgc
     1981 ccatgtggtg acatccacga cccacaagac gttgcgcggc ccgcgcggcg gtctcattct
     2041 caccaatgac gaggcgatcg ccaaaaagct caactcggcc gtgttccctg gcctccaggg
     2101 cgggcctttg atgcatgtga tcgcggcgaa ggccgtcgcc ttcggcgaag cgctgaagcc
     2161 cgaattcaag atctatgcaa agcaggtcgc agacaacgcc aaggctttgg cggcttcgct
     2221 caccgagcgc ggctatgcga ttacgaccgg cggcacagac aatcacctca tgctcgttga
     2281 tcttcggcca aagcagctca ccggcaaggc agcggaggca agtctcgggc gcgcggggat
     2341 cacctgcaac aagaatggcg tgccgttcga taccgcaagc ccgatggtga cctcgggcat
     2401 ccggctcggg acccccgccg cgacatcgcg cggttttggc atcgcggaat tcaaaaaggt
     2461 cggcgcgctg atcgccgaaa ctctggatgg cctggccaag aatggggacg ctggcaatgc
     2521 aggggtcgag gccaaagtga aggccgacgc agcagcattg accgagcgct tcccggtcta
     2581 ttgagcctgc gatctttggt gccgcaagac caggtaacgt gcgccagcgt gatggcgtgc
     2641 gccatcgttc caggatcttg ctttgaggcc tatccgccat gcggtgccct tactgcggca
     2701 gtctcgaaac ccaggtcaaa gactcgcgcc cgagcgaaga ttcctctgcc attcgccgcc
     2761 gccgggcctg tcccgattgc ggcggccggt tcaccacttt cgagcgcgtg caattgcgcg
     2821 agctgatggt ggtgaaaaag tccggccgcc gcgtgccttt cgaccgcgac aagctcatgc
     2881 gctcgctcga aatcgctttg cgcaaacggc ccgtggatcc ggaacgcgtc gagcggatgg
     2941 tgaatgggat cgtgcgccag cttgaaagtc agggggatag cgacattcca agcgaacgga
     3001 tcggcgagct cgtgatggaa ggacttcgcg cgctcgacag cgttgcctat gtgcgctttg
     3061 cctcggtcta ccgcaatttt agagaggcgc gggatttcaa cgctcttatt gatgagcttg
     3121 cgaatggcgc cgacggaacg cccgaccagc caacgactcc gtctccaaca aaggcgcgtt
     3181 cgccgagcca ggcatgagcc aatctaacga ggacctgcgg ttcatggctg cggcgcttgc
     3241 catcggacgg cgggggctcg ggctttcagc gcccaatccg tctgtcggag cccttgttgt
     3301 caaggacggg gttatcttgg cgcgcggttg gaccaagccc ggcgggcgtc cccacgcgga
     3361 aaccgaagct ttgcgtgacg cgagtttgga tgcgacaggt gcaacgctct atgtgacatt
     3421 ggaaccctgc agccatcatg ggcagacacc accttgcacg gatgccatag tcgcgtcggg
     3481 agtatcccgc gttgtttatg caatcgatga tcccgatccc cgcgcatgcg gatccgccga
     3541 gaaaatcttg gagacggcag gtattggcgt gaccaagggt gttctagaag tcgaggcgaa
     3601 gcacgccaat ctcggccatt ttttatgtgt cacacaaaag cgcccgatga tcaccataaa
     3661 gctcgtattg acggcggatt tttacgcggc gaggcagggc ggcgagccgc gtctcctgat
     3721 taccggtgaa cccgccaatg gcttggtgca tatcatgcgc gcgatgcatg acgcgatcat
     3781 gattggcatt ggcacggttt tggcggatga ccccctttta acggtgcggc ttcctggcgt
     3841 tgaggaccgc aagcctatgc ggattgtgct cgatggcgac cttcgcctgc cgccgcaatc
     3901 ccggctggca gcgacggccg cgaaaatccc agcacttgct attgctgggg aaggcgcgag
     3961 cgaagatcgt gcggcgcgct tacgtgtggc gcatgtcgaa attgcgcatg tccgccggga
     4021 cgcggcaggg catgtcgacc ttgaggctgc gctcaatctt ctggcgtcgc gcggcctcac
     4081 ccggattttc tgtgaggggg gtccgcgggt tgctgcggca ttgatcgggc aaaggcttgc
     4141 cgacgaagtc tttctcttca ccgctccaaa gccgctcgcg caaaaaggtg ttttgggtct
     4201 cgatgcggcg acagcctccg tgctcgccga tcctcagcaa tatcttcttg ccgagagccg
     4261 aatgattggt gctgaccggc tcacccgccg tgaaagagtg gactaaatgt tcaccggtgt
     4321 cgttagcgac gtgggcgtag tcgaagaggc cgaggcgagg gcaaatctgc gccgtctgca
     4381 tattgcctgt gcctatcccg cggagagggt cacgcccggc gcgtcgatcg cctgcgccgg
     4441 tgtttgcctc acagtaaccg cgagcggcag cagtggcaag ggcggctggt ttgaagtaga
     4501 cgccggtttg gaaacgcttg ccaggacgac ggcaaagcac tggaaaaagg gtacaaggct
     4561 taatctcgaa cgagccttga gattaggcga tgaattgggc ggccatatgg tgagcggcca
     4621 tgtagatggc gttgccgaaa tcgtttcgat cgaagagttt gagggcatgc ggcgcatcgg
     4681 cttcgaggcg ccccatgatc ttgcgcggtt catcgcggaa aaaggttcgg tggcgcttga
     4741 tggcacatcg ctcacggtca attgggtgga aggggatatt ttttcggtgc tgcttattcc
     4801 gcacacgctg aaggtcacga cctgggaaag cgcgcagata agcatgggcg ttaatctcga
     4861 agtcgatctc ttggcccgtt atgccgcaag gctcaccgag gcccgcggcg ggcagcagca
     4921 aggcgcatag ccgcgcgcat ggactgcggc gtgccccgag ccgttcgcac caaattcctt
     4981 cgcggcaaag tctacttcgt cttttaggcg aatcagctgc tcgccgttac actggctcct
     5041 cgattctgcg agtttagaag cggggatttg ggtgcaaacg ggccaagagc ttttgcaaaa
     5101 atgggatgcg cgggctcccc gcctttacct gcgcatggaa gttaccggcg aagggctcgc
     5161 gctcggcgcg cggacgattt tggcgaaaat ggcgcgggac gagagaggcg cgccgtttct
     5221 tttccttgaa gatgagcaac gcaccatagc gctgctcacg accgcctatc agcggccttt
     5281 cgaaccatat cttctcgcca agctcagccg cgcctgcgaa ctttggagcg agaacgaaaa
     5341 agcccttgcg catattcatt tgacccatgc cgggttgccg ccctgcgacg aaggccaggc
     5401 tttgcgccta tttgttgccg aggaacttct cgaatcagaa actacgcctc acacgctctt
     5461 aaaagcgcag gggtttgata cagcggcgtt ggatttatcg aagttcagtc cggcgcagcc
     5521 gcgcgtctcc tccggcaacg gacgagagag cgggcaatgg acaagcggcg atgccgatat
     5581 cagcccggtc gcatttcgca gccccaaaga aagacgcggc cacagaggtg gaggcggcgt
     5641 atttgacgcg atccgctcct tttttggacc ggctacgaga agagcgaaag ccgaaagaaa
     5701 gcgaggctgc gacggagaga aaaccgggtc aggaagacgg ctccaaacct gaaagcgagc
     5761 cgcctaagca gtcgctgcca aagacggtaa ggtcagcccc aaaaccgtct gatttcgtag
     5821 gccaagactt cggcaagctc ggagtgggca tcgaaaagcc ggaactcgat atccacgaaa
     5881 ttgatggcca cgccatccga agaatggctg agcgtggggt gtcattggat gatatattca
     5941 agacggtcaa tcatccgttg atcgtcctgc aacaggcgag agggagagta ttttacctgt
     6001 cgaacaaggc cgcggtcgta attaaccctc gtggaagagt cataagctct tactccactt
     6061 cggattttga ttcaaaaata aaggcacttt tggagcacgt gtatgaataa cgaaaaagac
     6121 agtgacgact acaaaaggac tcaagaaatt cgagccattc tcctcaaaga ttgggatcct
     6181 ctgggaatcg gcgacaatcc aaatcttgcc gacgaatacg atcactacat tccagctatt
     6241 cttcgcctgt ttgacactca cggtacggtg gaggaactag aacggtatct agctgacatt
     6301 gaagaaaaat gggaactaac tccggatccg gccgcttggc gagcggccaa gaagatcttt
     6361 gatgctttga aagaaagtga gtaaccccac ggtgctcgtc gagcgcgcca cggcccactg
     6421 gatacgcgga ggtggcgctt agcaccggaa cggtccctgc gaaaatggtg cgggatggac
     6481 gcagcaagcc aagtctcgcg cttgacgacg agccgcgcat cgtggcgctg ttcgccaccg
     6541 cgtatgagca gccagtcggg cctcaagtcc tcgccaagat caggcgggct tgcgagcttt
     6601 ggaacgcggg tgaaaaggcg cgagcgtgcc gccttgcgaa ctcgttgcag ccgtggtcgc
     6661 gccgccgact ctgctaaaag cgcaaggctt tcatccgcca ccgctcgaca tgctgaaatt
     6721 caatccggcg caaccgcgcg tcccctccgg caacggacga gagagcgggc aatggacaag
     6781 cggcgatgcc gatatcactc cagtcgcatt tgagggagac acggccacag cggtggaggc
     6841 ggcgtatttg atgcgatttt tttgaccggt tgcaggaagg gcgaactact aagttaagcc
     6901 agcctccacc agagagaaaa ccagatcacg aagagggaat caaacctgaa ggcgaggcgc
     6961 ccaaaggatc accgcctagt cggtaacctc tgcctcaaag tcatctgatt tcgtaggcca
     7021 agacttcggc aaactcggag tgggcattga aaagccgaac ctcagtatca gccggctttc
     7081 tgaccacggg gagtttaggg cgcaaacacg aggtgtgcca gtcgatgatt tgcaaagcac
     7141 agtcgaaaac ccgcttcttg ttcttcgaca atcatcagga agattctttt atctttctga
     7201 caaagcggtg gttgtcttgg accgcgaagg atcagttgta accacctatc cagcatcgaa
     7261 gttcgataca acgattagaa cgatattaga ccagatatct aaaaggggcg gaaaatgagc
     7321 gatgaagatc gagcccagcg ggtgcgcgac actcttctca aagattggga cccgcttatc
     7381 gtgggtgaca atccacatct taccgatgaa tatgacgacc tcattccagg gatccttcac
     7441 ctgctcgacg ctcactgcac agccgagcag ttgggacacc atctcgcgtc aatcgatgcc
     7501 aaatggggcg gtgagcccgc ttggccgaat attgagcgga ctgtcgggaa
//
LOCUS       DATMPU010000099         5474 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_2245856,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000099 DATMPU010000000
VERSION     DATMPU010000099.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 5474)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5474)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5474
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2245856"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            232..668
                     /locus_tag="VNY06_02265"
                     /pseudo
     CDS             232..668
                     /locus_tag="VNY06_02265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019997028.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
     gene            complement(679..972)
                     /locus_tag="VNY06_02270"
     CDS             complement(679..972)
                     /locus_tag="VNY06_02270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012589122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX13695.1"
                     /translation="MILSVNPLIAMGVLASTAATDAAYVFFNAAVTSRRRIWAANWSA
                     IWYLLSAFAVISYTENAIYVLFAAAGSWLGAFASVSWLGHGSASVAMGPKSPP"
     gene            complement(1009..1476)
                     /gene="rnhA"
                     /locus_tag="VNY06_02275"
     CDS             complement(1009..1476)
                     /gene="rnhA"
                     /locus_tag="VNY06_02275"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012385299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HWX13696.1"
                     /translation="MTGEVSIWTDGACSGNPGPGGWGAILSFGGHEKELFGGEASTTN
                     NRMELTAAIEALETLKRPCAVHLHTDSNYLKAGITAWIVTWKRNGWRTADKKPVKNVE
                     LWQRLDMAAARHDIDWRWVKGHADDVMNERADALARKGMRAFNAPGPVESPSG"
     gene            complement(1473..2438)
                     /locus_tag="VNY06_02280"
     CDS             complement(1473..2438)
                     /locus_tag="VNY06_02280"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012385300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HWX13697.1"
                     /translation="MAVYTEVNDADLARFLVDYDLGQVLALKGIAEGVENSNFFLHTG
                     RGYFILTLYEKRVDERDLPFFLGLMEHLAAHGLKCPQPVKNQHGGALGRIAGRPAALV
                     TFLEGMWRRNPSVRCCAAVGGALGKLHRLGASFAGNRANTMGLATWPAMFQAAQPRAD
                     ELQPCLAAEIAKELDFLAGNWPRHLPSGIIHADLFPDNVLFLENEVSGLIDFYFACTD
                     QLAYDVAICLNAWCFEPDGSYNITKGLAFFSAYESIRRLTAGEGEAFLVLARGAALRF
                     TLTRLVDWLNVPASALVHPKDPLEYVKKLRFHQQAHNARELGLLR"
     gene            complement(2570..3298)
                     /locus_tag="VNY06_02285"
     CDS             complement(2570..3298)
                     /locus_tag="VNY06_02285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012590299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF502 domain-containing protein"
                     /protein_id="HWX13698.1"
                     /translation="MNMPPDDTPPSDGYGPTATVKGFGLGGRIRNWFLTGVIVAGPLA
                     ITVYIVWWFVDTIDNWVKRIVPNGFWPDSYLPLQLPGLGVVLAFLGLTLLGFLAANLA
                     GRTFIRFGEAMLARMPVVRSIYKSAKQIFETVFSSSGTTFRRVGMIEFPGEGKWSIVF
                     ICTPPGASFAGHLSSKEYISVFLPCTPNPTTGFYFFLPAREVIELPISPDDAVKLIVS
                     CGVIQPDASAVIAQIGNNPKRISA"
     gene            complement(3691..4164)
                     /locus_tag="VNY06_02290"
     CDS             complement(3691..4164)
                     /locus_tag="VNY06_02290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX13699.1"
                     /translation="MNIVCFGLILFLISHSSVFAKQDGVPHFDIEHTCRAARSYAGAD
                     KEVAYKGCLKDEADAREQLAQKWTSFKSADRSDCVLQGSEPMPSYVELLTCLEMSAEV
                     GVLNAPNGARGNKALGSVRQNPLGPGQGGPPSGTPAPTPEMPVPTPENGGLAKPN"
BASE COUNT         1257 a         1516 c         1518 g         1183 t
ORIGIN      
        1 acggtgaaaa gtgaacggca aagttacgag ttcgccgtcg atctagatgc tgcttccgat
       61 caaaggaaag acgctcaatt ggcaccgaag tctgttccgt gatctgatca aaatgaataa
      121 ataacaagaa tcgcccttcc agttatgctg cagctttttc gctgcccgcg ggaaccgtca
      181 tggcatgatg ccaatctgag tggagctttg gcgtttgata aaggagcaca aatgacaatc
      241 cagattggcg attccatccc caagacatct tttttaaagg caaaaaaata gtcctctttg
      301 gcgtggctgg tgccttcacg ccagtttgcg acaaaaacca tttgccaggg tttctcaaca
      361 atatcggcgc gttcaaaggg agaggaatcg atgacatcgc cgtaacaagc gtcaatgata
      421 ttttcgtcat ggatgcttgg gcgaagggta ccggagccaa cgacaagatc acttttctcg
      481 ctgatggaaa cggagacttt gcgcgcgctc tcgggctttc gcttgatctc acagcgcgcg
      541 ggctcggcat tcggtcgcag cgctatgcga tgatcgtcga cgacggcgtg gtacgaaaac
      601 tcaacgtcga agcgtcaccc ggcaaggtcg agacctcgag cgcggaagcc cttcttaagg
      661 acctttaggc atctcgcgct acggtgggct cttgggcccc atcgcgacgc tggcggagcc
      721 gtgcccaagc cagctgaccg aagcaaatgc accaagccac gagcccgctg cagcaaacag
      781 gacgtagata gcattttccg tatagctaat gactgcaaat gcagagagca ggtaccaaat
      841 ggcgctccaa ttcgccgccc agatacggcg tctgctggtc acagcggcat tgaagaacac
      901 ataggctgca tctgtggcag cggtggaggc aagcacgccc atcgcgatga gcgggttaac
      961 ggatagaatc ataggaagtt cttcccttag agctgcaaca attccggttt atcctgaggg
     1021 gctttcaaca gggccaggtg cattgaacgc gcgcatcccc ttccgcgcga gggcatcggc
     1081 ccgctcgttc atgacatcgt cggcatggcc cttcacccaa cgccaatcaa tgtcgtggcg
     1141 cgcggcggcc atatcgagcc tttgccatag ttcaacattt ttcacgggct tcttatccgc
     1201 agtgcgccag ccattgcgct tccaggtaac gatccatgcc gttattccgg ccttaagata
     1261 gttcgagtcc gtatggagat ggacagcgca aggccttttc agcgtctcca gggcctcgat
     1321 cgccgcggtt aattccatgc ggttattggt cgtcgaagct tcgcccccaa atagctcctt
     1381 ctcatggcct ccgaagctga ggatagcgcc ccagcctcca gggccagggt tccccgagca
     1441 ggcgccatcg gtccaaatcg agacctcgcc tgtcatcgca acaaaccgag ttcccgggcg
     1501 ttgtgcgctt gctgatggaa gcgcagtttt ttgacgtatt cgagcggatc cttcgggtgc
     1561 accagagcac ttgctggcac attcaaccag tcgactaggc gagttagggt gaagcgaaga
     1621 gctgcgcctc tcgcgaggac caaaaatgcc tcgccttcgc cagcggttaa tctgcgaata
     1681 ctctcatagg cgctgaaaaa tgctagtccc ttggtgatgt tgtacgaccc gtccggctcg
     1741 aaacaccagg cgttgaggca aatcgcaaca tcgtaagcaa gttgatccgt gcaggcgaag
     1801 tagaagtcta tgagccccga tacttcattc tcaagaaata aaacattgtc gggaaagagg
     1861 tcggcatgga tgatgccgct tggtaaatga cgcggccagt ttccagcaag aaagtccaat
     1921 tccttggcta tttccgcggc gaggcatggt tgcaattcat cggcacgcgg ctgcgccgct
     1981 tgaaacatcg cgggccaagt cgcgaggccc atggtattgg cccgattgcc agcaaaactg
     2041 gcaccgaggc gatgaagctt tccgagcgca ccgccaacgg ctgcgcagca ccggacgctt
     2101 gggtttcttc gccacatacc ttcgagaaat gtcaccaatg ccgccgggcg tcccgcaatc
     2161 ctgccgaggg caccgccgtg ctggtttttg actggctgcg gacatttcaa tccgtgtgca
     2221 gccaaatgct ccatgagccc gaggaaaaac ggcaggtctc tttcgtcgac tcgcttctca
     2281 taaagcgtaa ggatgaaata gccgcgccct gtgtgcagaa aaaaatttga attttctacc
     2341 ccttcggcga tcccttttag ggcaagaact tggccgagat cataatcgac aagaaaccgg
     2401 gcaaggtccg cgtcattgac ttcagtataa acggccatgg cgcatccgcg cggagagata
     2461 ggcgggcggc aggtcccccg gcacgccctc gtgttagctg gctctcagcc caaggtcaaa
     2521 cacgggggcg gcggcacggc aaggaatggg gcggaggtcc actcgcagct catgcagaaa
     2581 tacgctttgg attgttgccg atttgggcaa taaccgcgct cgcatcgggc tggatgacac
     2641 cgcaagatac aatcagcttg acggcatcgt cggggctgat tggaagttcg atgacctcgc
     2701 gcgccggcag gaagaagtaa aagcccgtcg tcggattagg ggtgcatggc aagaataccg
     2761 aaatgtactc tttgctcgag aggtggccgg cgaaggacgc gccgggcggc gtacaaatga
     2821 aaacgatgga ccatttcccc tctccaggga actcgatcat ccccaccctg cggaacgtcg
     2881 tgccagaact ggaaaatacg gtctcaaaaa tttgtttcgc gcttttatag attgaacgca
     2941 cgaccggcat gcgtgccagc atcgcttcgc cgaatctgat gaaggtccgg cccgcaagat
     3001 ttgcggcaag aaaaccgagc aaggtaagcc caaggaaagc caacaccacg ccgagcccag
     3061 gcaattgcaa cggcaaatag ctatccggcc aaaaaccgtt aggcacgatg cgtttgaccc
     3121 aattgtcgat ggtatcgacg aaccaccaca caatatagac ggtgattgcg agtggacccg
     3181 cgacgatgac cccggtcaga aaccaattgc gaatgcgccc cccgaggccg aaacctttaa
     3241 ccgtcgcggt cgggccgtag ccgtctgagg gaggcgtgtc atctggcggc atattcattt
     3301 ccattcgccc agtggcattc gaacccatgt gacaggttta gctcttttgc ggcccgaaaa
     3361 ttgcacggcc gatcagtttg gccgaaaatg ttttcaaatt ttcgcccgtt cggaggcatt
     3421 ctgcctattt ccgcgtgaca ttgcgtgaaa cgtaagctac ggttacatcg taagccgttc
     3481 aaggccgaag ccgtgataag ttcggcatag cagtccaagt cttgggagga cgcctgttct
     3541 gtcgttgtag caccgcaacg tggtgcagcc ttcataccta aagacaaggc cggcgaacag
     3601 gttatggaca ggactctcgg aggcggagat acgcaaagta tctctgcctc tttttttagc
     3661 aaaattacgt gccggcggga cgcagaggtt tcaattgggc ttcgctagac ctccattttc
     3721 gggcgtcggc acaggcattt caggcgttgg cgcaggcgtt ccgctcggtg ggccgccctg
     3781 gccgggcccg agcggatttt gccgcacgga gccaagggcc ttattgccgc gggcgccatt
     3841 aggcgcattg agcactccga cttccgcgct catctcaagg caggtcaata gctcgacgta
     3901 gcttggcatg ggttcggagc cttgcaggac acagtcgctg cggtctgccg acttaaaact
     3961 agtccatttt tgtgccaatt gctcgcgcgc atcagcctcg tccttcaaac agcctttgta
     4021 agcgacctcc ttatcggcgc cagcatagct gcgcgctgct cgacatgtgt gttcaatgtc
     4081 gaagtgtggc accccgtcct gctttgcaaa tacgctgctg tgcgaaatga ggaataggat
     4141 caaaccaaag caaacaatat tcaagcgaat gagacgcctc gcgttagtgt aagccgtttt
     4201 ggtcctgtag atcaagcgcg tcgtccctct tgtcgcaacc accacggaca aaaccccaag
     4261 attacgcgga tgttggaaat tcgtttccca cttatgggcc gtttgcgtct agctttatat
     4321 tttttttgta tcattcttga tcagttcaaa gttcgcatgg tgagtattat gaagtaaatg
     4381 atggcgaact gcatcaaatt tattgggaag tgtcatgcgc aagaatggct gccgcaaggt
     4441 ttatttgcga aaaaaaatct cgttacattt ttgaaatggt ttgcggcttt cctagctgcc
     4501 gcgataggcg gcttcggcac cattacaccg gctatggcag cccccttcgc ttatgtcggt
     4561 ggcggagagg gcctaggtat atctgtaatc gacataagct ggtcgccact atttgggcta
     4621 agcgcgcaat tggtggcgta gccgtcagcc cggatgggaa acgcgcctat gtcacagcaa
     4681 atggcccgtc ggctggcact ggctccgtcg aagtgatcga taccgtcagc cacaccgtga
     4741 tagccgacgt cccagtgcca acactagccg ggggcggagg catcgcaatc gctgtttgga
     4801 tcgcaattaa tcccgatggc aagcacgcct atgtgacgac attgattgac ttggcagcaa
     4861 caaggtggtg ggcacaagta ggtggcagcc ccggggccat agccgtcatg ccagatggga
     4921 aacacgctta tgtcacgagc gacgcttatg tcacgagcgg cacaccagcg ttgcgttgtt
     4981 gtcatgagcg ccagcgccgc catccatcgg cagtgccccg aaccaagatg cgtttctgtt
     5041 tcaatccaac ttcactttga gcagcacgca aggcaaagac cgccagattc ctttgagaag
     5101 caagcgaacg ggtcgtttac cttcgctggc tccgtcattg gcgtgaccaa gcaagcgacg
     5161 atcaagccga cgcgcacgct gcgctattcg ttctccgcca cagcgcagcc gccaatttgg
     5221 ccgggtcggt taatcccatt caggtgtcgc tgatcatcgg aaacgacagc ggaacgatgt
     5281 cgttcaatgc cgtcgctacg aaggtagtaa cgaagtagtt ggtccgacac cttcagttag
     5341 gggaagaggc ggtccggctg ccggccgttc gcgatcgtca cttctcatag tgatccgcga
     5401 caaaccggtt caacagccgg acgccaaacc cagagcccca gctttgattg atttcgctcg
     5461 ccggcgaacc catg
//
LOCUS       DATMPU010000100         4611 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_692789,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000100 DATMPU010000000
VERSION     DATMPU010000100.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 4611)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4611)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4611
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_692789"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..241)
                     /locus_tag="VNY06_02295"
     CDS             complement(<1..241)
                     /locus_tag="VNY06_02295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012591089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase"
                     /protein_id="HWX13700.1"
                     /translation="MTKFAAGDMEICAVPVQGPDPEVFREAMSRIAASVHIVTTDGPA
                     GLAGITATTIASITLEPPMMLFCIKKTSPSAVRIIE"
     gene            complement(656..1387)
                     /locus_tag="VNY06_02300"
     CDS             complement(656..1387)
                     /locus_tag="VNY06_02300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012385195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HWX13701.1"
                     /translation="MTNDVTLPAAALAGLLSFLSPCVLPLVPPYLTFIAGTTIEEVAV
                     HREVRARRSILLAAVQFVFGFSTVFVGLGATASSFGQLLHQYSSRLSLLAAVAIILMG
                     LHFLGVFRFAVLYREARVEVQRPRGVWGSYVMGLAFGFGWTPCIGPILAAILAVAASQ
                     NTVGKGILLLTFYSAGLGMPFLLAALAFEPFVRVIKRFRPYYRAIEQVIGVLLVATGI
                     GFLTGSVQDVSAWLLQNFPGLANLG"
     gene            1653..3107
                     /locus_tag="VNY06_02305"
     CDS             1653..3107
                     /locus_tag="VNY06_02305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012591097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HWX13702.1"
                     /translation="MTVFLVSFCNILFSFAIGLLVAKFLGPEEYGRFALAYATAIFVQ
                     TGFFDWVRLGATRFYSERIRSEEPAVRATLDFGFAIITLSLAAGASLLLLSGIKFTLS
                     YGLIALALGVAVANGIFDYRMALVRARFDDRLFTRLLLVKNIAALALMGGGAFLFSSA
                     KTTLIGTILSLSASVMTARAALHDPGSKPRFARFSIARSLMTYSLPIVGANLLYLAIP
                     LANRSILAIVEGFSETGQFSLAYDFGVKAIQAIGSALDIVLFQLAVATHELWGPVQAR
                     RQIARNLTIVIAMVLPACTGLWLTLPSIEHLAVPEAFRGHFDMLLPLMMTGLFCMAVI
                     QYGINPIFQIEKKTAPLVVAALAACAADPLLLFMLPRDSGASRLAIAQSGALLLALAV
                     LICSAARAWRPWPRTRDFAVIVFGNAAMSVIVLPLRGQDPGVATLILQVAAGVATYAC
                     VLLGLDLAGLQSLTISRLRLTFARVKTLPTLRDR"
     gene            complement(3201..4133)
                     /locus_tag="VNY06_02310"
     CDS             complement(3201..4133)
                     /locus_tag="VNY06_02310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018261994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008276 - protein methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006479 - protein methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HWX13703.1"
                     /translation="MTAAVLEGLPPNGAAHVMRLACHEAAARGIADIIVETFDPATTA
                     ASAFEERPDANTWKSEGPWIVEAYFGTPPDDSKVRALVAIVAGEAAARKAVFSRIDER
                     DWVKSSLEGLKPVRAGRFIIHGAHGRDAVKSNDIAIEIEAALAFGSGHHGSTRGCLIM
                     LDRVARKSRPRAILDLGTGSGILAIAAAKLFRRKVYAGDIDPVCVHTATANAKQNGAA
                     TYVRPVLARGTAHQLLRQGAPYDMVMANILARLLRDLARDIARRTLPGADIILSGLIA
                     RDVQGVIAAYRAQAIAVVQRIDVEGWATLLMRRN"
BASE COUNT          976 a         1331 c         1280 g         1024 t
ORIGIN      
        1 tctcgataat ccgcaccgcc gagggcgaag tttttttgat gcaaaacagc atcatgggcg
       61 gctcgagcgt gatggatgca atggtggtcg cggttatccc tgcaagaccc gccgggccat
      121 ctgtcgtaac aatatgaacg gatgcggcga tgcggctcat tgcctcgcga aagacttccg
      181 ggtcagggcc ttggaccggc acggcacata tctccatgtc tcctgctgca aatttggtca
      241 tggccccgca aagctccttc tgcatcgaga ctacaacgtg gcgttgcttg gagtcaccag
      301 cttgctctaa atcattgttt tgctgcatgc ccggcgcaaa actggttctc actgtcgacg
      361 cgctctagga ctagcaggca atgataagtt ttttgattgc aaagcgatca tgagccttgt
      421 gaatcttggt tggaaacata gaccaaagca gcagttgcgt gcgtatcgca cggtctattc
      481 aaaacttcgt atctcatcca ctcccggtat gcacatcagg cgattccgga acggcggaaa
      541 agcagagccg taccatccgc catggcatag gcgatgcatc gcggcaaggc gttcagactt
      601 gcctttggcc gatatgataa tttaagtatt tgcaaactct tacgcttcga tgcagctaac
      661 ccaaattcgc aaggccagga aaattttgca gcagccacgc cgagacatcc tgaaccgaac
      721 cggtcaggaa gcctattccc gtggccacga gcaacacgcc aatgacctgc tcaattgccc
      781 tgtagtaggg acgaaacctc ttgataacac gtacaaaggg ctcaaaggcg agtgccgcca
      841 gcaagaacgg catgccgagc ccagccgaat agaaagtgag cagcaaaata ccctttccga
      901 ccgtgttctg ggaagcggca actgcaagga tcgcagcgag aattgggccg atgcaagggg
      961 tccagccgaa gccaaaggct aaccccataa cataggagcc ccagacacct cggggtcgtt
     1021 gcacctcgac tcgagcctcc cgataaagaa ccgcaaagcg gaatacgccc aagaaatgca
     1081 agcccatcaa aatgatcgca acagcagcga gcagcgaaag ccgcgacgaa tattgatgca
     1141 aaagctgacc gaaactcgac gccgtagcgc caaggccaac gaaaacagtg gaaaagccga
     1201 agacgaactg cacagcggcg aggaggatag accgccgcgc gcgaacctcc cggtggacag
     1261 cgacctcctc aattgtcgtc cccgcaatga aggtaagata aggcggaacc aaaggcagca
     1321 cgcaggggct caagaaagac agaaggcccg caagcgcggc agctggaaga gtcacatcat
     1381 tcgtcatgtg cccttctagc ttgttccaag acgatgcaaa agcgtcacgg tcccgaaaac
     1441 ggctgccgga ccggccgttt cataaaatta acgtgacgcc ctataagatg cggtagcagt
     1501 ggttcagaac atgttcagga aatattcatg gaagtaatcg ttagcctcgc cgcgacgctg
     1561 agagctgcca tcactaggct tttctgcttt gttcaacttc cggcatgcgc aagtgccaac
     1621 accgcttcac atgggccggg tgcacacttt cgatgacggt tttcctcgtc tctttttgca
     1681 acatcctgtt cagtttcgcg atcgggctgc tcgttgcgaa gtttcttggg ccagaggaat
     1741 atggccggtt tgcccttgcc tatgcgacgg cgattttcgt gcagaccggc tttttcgatt
     1801 gggtccggct cggtgccacg cgtttctatt ctgagcggat aaggagcgaa gagccagccg
     1861 ttcgggcgac cctagatttc ggttttgcca taattacctt gagcctcgct gccggggcaa
     1921 gtcttcttct gctaagtggt atcaagttca ctttatctta cggtttgatt gcattggcgc
     1981 ttggcgtcgc ggtcgccaat gggattttcg attatcgcat ggcgctggtt cgtgcccggt
     2041 ttgacgaccg tctttttacc cgccttttgc ttgtcaagaa tattgcggct ctcgctctca
     2101 tgggcggcgg agcctttttg tttagctcgg ccaagacgac cctcattggc acgattctca
     2161 gtctcagcgc ctcggttatg accgcgcgtg cggcattgca tgatccggga tcaaaaccgc
     2221 gcttcgcgcg gttttccatc gctcggtcgc ttatgaccta tagcctgccc atcgtcggtg
     2281 ccaatctgct ttaccttgca attccgctcg ccaatcgctc aattcttgcc attgtcgaag
     2341 gtttttccga gactggacag ttttctcttg cctatgattt cggagtcaaa gcgattcagg
     2401 cgatcggctc agccctcgac atcgtcctgt ttcaactcgc ggtcgcgaca catgaattgt
     2461 ggggtccggt gcaggcccgg cgacaaatcg cccgcaattt gaccatcgtc attgccatgg
     2521 ttctgccagc atgcactggt ctttggctga ccttaccgtc aatcgagcat ctggccgtgc
     2581 cagaggcttt tagggggcat ttcgatatgc tcttgccttt gatgatgacg gggctctttt
     2641 gtatggctgt tattcaatat ggtatcaatc cgatcttcca gattgagaaa aaaaccgcac
     2701 ccttggtcgt ggcagcactc gcggcttgcg ccgccgatcc actcttgctc tttatgttac
     2761 cccgggatag cggcgcatcg cgccttgcaa ttgctcaatc tggcgcactg ctgcttgcac
     2821 tcgccgtgct catttgttct gcagcccggg catggcgacc ctggccgcgt acccgcgact
     2881 ttgccgttat cgttttcggc aatgccgcga tgtctgtgat cgttctgccg ctgcgcgggc
     2941 aggatcctgg tgttgcaacg ctcatcctac aagtcgcagc tggtgtcgcc acctatgcat
     3001 gcgtcctatt aggcctggat cttgctggtt tgcagagcct cacgatctcg cgtctgcgcc
     3061 ttaccttcgc acgggtaaag actttaccga ctctacgcga tcgctagagc gtggccggaa
     3121 aggtgggggc cggctttgcg tcccgacgcg taacaaaaca actagagcaa gaggacgatt
     3181 cgaagaaaag tcattttgct ttagttgcgc cgcatgagca aagttgccca accttcgaca
     3241 tcgatccgct gcaccacggc aatggcctgc gcgcgatagg cggcaatgac gccttgaaca
     3301 tcgcgcgcga ttaggcccga cagaattatg tctgcgccag gcagggttcg tcttgctatg
     3361 tcacgggcga gatcacgcaa caggcgcgca agtatgttgg ccataaccat gtcgtagggc
     3421 gccccttgcc gcaatagctg atgcgcagtg cccctcgcca aaaccgggcg cacgtaggtt
     3481 gcagcaccat tttgctttgc atttgcagtc gcggtatgca cgcaaacagg atcgatatcc
     3541 ccggcatata ctttgcgccg gaacagcttg gcggccgcga tggcaaggat ccctgagcct
     3601 gtaccaagat cgagaatggc gcgagggcgg gatttacggg ccacgcggtc cagcatgatg
     3661 aggcaaccgc gcgtcgaacc gtggtggccg ctgccgaagg caagcgcagc ctcgatctcg
     3721 attgcgatat cattggactt gacagcatca cgcccgtgag caccatgaat aatgaaacgc
     3781 ccggcccgaa cgggtttcag cccttcgagg gaactcttga cccagtcccg ctcatcgatt
     3841 cggctgaaaa ctgctttccg cgcggcggcc tcccccgcca caatggcgac aagcgcgcga
     3901 accttcgaat catctggagg ggttccgaag taggcctcga caatccacgg accctcgctt
     3961 ttccaggtgt ttgcgtcggg tctctcttca aaggccgagg cggcggtcgt ggccgggtca
     4021 aaagtctcga caatgatgtc ggcgatgccc cgcgccgctg cctcatggca ggcaaggcgc
     4081 atcacatgcg cagcaccatt cggtggcaag ccttcaagca cggcagcagt caaccgttac
     4141 cccattgtcc gattctgccc tcaaatctta ccaaaatgtt tcacgggcta taatattcac
     4201 gggctataat aggagaacac cccccgcaca aaacagcgtc acagacgtaa ccctagcaat
     4261 acgcgaggcg cacgttacaa tattacacta tggttggtat ggttggtgtg aatggggcgg
     4321 gtgacgcgtg gcgtctaggt ccgtgacggc gcccgtgggc cagtcctgcg gccagtccta
     4381 cggccagtcc tgcggttggt tctggggttg gttctggggt tgcggcttga ggggctgggc
     4441 gagggtcgcg ggcgtgcggc cagtgcccgt ccctagcacc ccgcccgcac cagccgcagg
     4501 cttggccgca cgtctccgtc tccgcgcgtt gggggagcgt ggacctgggg cattagcacg
     4561 ccctctttca taaccaatgc accccgcgcc ttagttgagg ctgtgggcca g
//
LOCUS       DATMPU010000101         4900 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_693298,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000101 DATMPU010000000
VERSION     DATMPU010000101.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 4900)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4900)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4900
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_693298"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>4900
                     /locus_tag="VNY06_02315"
     CDS             <1..>4900
                     /locus_tag="VNY06_02315"
                     /inference="COORDINATES: protein motif:HMM:NF016724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HWX13704.1"
                     /translation="VEAMWSALERLGVGAGARILEPSVGVGNFLGLMPQSLMPGASRA
                     GVEVDSITARIARLLYPDSRIFESGFEEVAFPDHFFDVAIGNVPFGDYGVHDDHYKPW
                     QTRCIHDYFFVKSLDTLRPGGVLAFITSRYTMDKEDDRIREHLASQADLLAAIRLPNT
                     SFKDNAGTVVTTDIIFLQKRGAGQAPANALWRQVLDYTYSPEQLFSFSLNEYYVRNPH
                     MMLGTMELVRGSYAREQPELIGDITKAGLEAAIAALPRDVYAPRAEPLAPTVLSFIGN
                     DGAFAGVKNGAFVIVDDVLGIRQDGQFVPASLTLKAEARVRGMMKVRGYVRGVFEIQL
                     VDNTDEVIAEARRNLNYAYDRFVEDFGYLSNVDNRRAFASDPDAPLLLSLEEDYDGAA
                     GRARKAAIFERPTLERYRPVEHVDTAAEALAVSLNEHGRLDWSRMEAVTGRAAAALQQ
                     ELGPLVYENPESGRWETADAYLSGNVRHKLAVARAAAAIDKKYHRNIAPLEGAQPADL
                     TPGEISARLGSTWIPTDDLGDFVADVIEASHTAVRVDYVPEVATWAVTAKPYAKSNVA
                     NTTTYGTERFTAIDLIEDALNGKVPTAYDTIPGPDGDKRVINEARTLEAREAQQKLKD
                     KFAEWIWQDAQRAARLAEIYNEKFNSVRLRSYDGAHMTFPGMNKSILRKGDLDPHQKN
                     AVWRILQGGSTLLAHCVGAGKTWIMTAAVMEARRIGLAKKSMVVVPNHLVEQWGKAFL
                     QLYPQANIFVAGKEHFQGDKRAKAMSRIATGTFDAVIVSHKSFELLPVSDALFDGYMQ
                     EELEALEKAIREASADKSSRRIVKILEADKKRLVKRIEDRAKREKKDKTVTFEELGIT
                     NIFVDEADLYKNLGFTTKMARVAGLPNSNSNRAVDMHIKTRYLRKQNNGKGVVFATGT
                     PISNTMAELYTMKRYLAPEALEAAGMAAFDAWAANFGETVTSLEVTPDGGGYRMKTRF
                     AKFVNLPELLTMFRDFADVQTADMLNLPRPALKGGQNKVVVAPSTPELKTYVASLVKR
                     ADRIKTGSVDPRDDNMLKITSDGRKAALDMRLVDPFATVHPESKVNLVINEVFTIWKE
                     TEQQRATQLVFCDLSTPSPDRFHVYGAIRSGLIERGVPVSEIDFIHDADTDAAKQALF
                     EKVNSGRGRILLGSTEKMGAGTNVQRFLYASHNVDAPWRPRDIEQRDGRILRPGNTHP
                     GVLIFRFVTEGSFDAYMWQTLEAKARFIQQVMNGETSVRTAEDITGGALTYAEIKAIA
                     SGNPAVMEKVKVDTEVRKLDSLRTSHAKRKFEIRRKIGVTQTRIARNEEQLAMVGKDI
                     ATREANMGEQFAMTLAGRRYTGKEGREQAAKELNGVILTLSGDAEAKCRGQIAGFDMM
                     TRATPDREVTAYLRGFAAYTFHHNLENPLGTIMSIERTLRGFEAIQGRLESDIARDQK
                     ELAEYRVESEKPFEYEEQLKQLLVKQAQLNAELDLGKDDKQAVTAESEGGAAKAVEGL
                     VAVVDGLMGGGQKSTKQQAHDKAQAAGNVETRDAEAYAAKDVALIDQARIPAELERQV
                     LEDQPVEIFRAASDGDKIHSYLQSLQQQQSVSAELQADRSEIIKEYDRIVDGRPEARK
                     ELEQTNPALVAARDKAVTELRRE"
BASE COUNT         1103 a         1384 c         1503 g          910 t
ORIGIN      
        1 gtcgaggcga tgtggtcggc gcttgagcgt ctcggtgtgg gagccggggc gcgcatcctc
       61 gagcccagcg ttggcgtcgg caattttctc ggcctcatgc cgcaatcgct gatgccgggg
      121 gcgtcacgcg ccggtgtcga agtggattcg atcaccgccc gtatcgcgag gctgctttac
      181 ccggattcca gaatcttcga gagcggtttc gaggaggtgg cgtttcccga tcattttttc
      241 gatgtggcga tcggcaatgt gcccttcggc gattacggcg tccatgacga tcattacaaa
      301 ccgtggcaga caaggtgcat tcacgattat tttttcgtga aatcgcttga tactctgcgt
      361 ccgggtggcg tgctggcttt tatcaccagc cgctacacga tggacaagga ggacgacagg
      421 atccgtgagc acttggcctc gcaggcggat ctgctcgccg ccatccgctt gcccaacacg
      481 agcttcaagg acaatgccgg cacggtcgtc acgaccgata tcatcttttt gcagaaacgc
      541 ggcgcggggc aagcgccggc caatgctctc tggcgacagg tattggatta cacgtattca
      601 ccggaacagt tgttttcgtt ttcgctcaac gaatattacg tgcgcaatcc gcacatgatg
      661 ctcggcacca tggagctcgt gcgaggcagc tatgcacgag agcagccgga acttatcggc
      721 gacatcacca aagccggttt agaggctgcg attgccgccc tgccgcgcga tgtgtacgca
      781 ccgcgcgccg agccccttgc tcccaccgtt ctgtccttta tcggcaacga cggcgctttc
      841 gccggcgtca agaacggcgc gtttgtgatc gtggatgacg tgcttggcat ccggcaggac
      901 ggacaattcg tcccggcctc tctgacgctc aaggcggagg cacgggtacg cggcatgatg
      961 aaagtgaggg gttacgttcg tggggtcttt gagatccaat tggtcgataa cacggacgag
     1021 gtcatcgcag aggcccgtcg caacctcaat tatgcctacg accgtttcgt cgaggacttc
     1081 ggctatttgt cgaatgtgga caaccgccgc gccttcgcca gcgatcctga cgcgcccctg
     1141 ttgctttcgc tggaggagga ttacgacgga gccgccggca gggccaggaa agccgcgatt
     1201 ttcgagcggc cgacgcttga gcgctatcgc cccgttgagc atgtcgatac ggcggcggaa
     1261 gctctcgccg tctcgctcaa cgagcatggc cggctcgact ggagccgcat ggaagcggtt
     1321 acaggccggg cggctgccgc cttgcagcag gaattggggc cgcttgttta cgagaacccg
     1381 gaaagcggca gatgggaaac cgccgatgcg tatctcagcg gcaacgtgcg gcacaagctg
     1441 gcggtggcgc gcgccgccgc tgcgatcgat aaaaaatatc accgcaacat agcgcccctc
     1501 gaaggcgcgc agcctgccga cctcaccccc ggcgagatct cggcgcggct cggctcgacg
     1561 tggattccaa ccgatgatct tggcgatttt gttgccgatg taatcgaggc cagtcatacc
     1621 gccgtgcgcg tcgattatgt gcccgaagtg gcgacatggg cggtgacggc caagccatac
     1681 gccaagtcga acgtggccaa taccacgacc tacggcaccg agcgtttcac ggcgatcgat
     1741 cttatcgagg acgcgcttaa tgggaaagtg ccgaccgctt acgataccat tccaggcccg
     1801 gacggcgaca agcgggtgat caatgaggcg cgaacgcttg aagcgcgcga ggcgcagcaa
     1861 aagctcaagg ataaatttgc cgaatggatt tggcaggacg cgcagcgcgc ggcgcgcctc
     1921 gccgaaatct acaacgaaaa attcaattcg gtccgcctgc gtagctacga cggggcgcat
     1981 atgacttttc cgggaatgaa taagtcgatc ttgcgcaagg gcgatctcga tccccaccag
     2041 aaaaacgcgg tctggcgcat attgcagggc ggcagtacct tgctcgccca ttgcgtggga
     2101 gccggcaaga catggatcat gaccgccgcg gtgatggagg cccgccgcat cgggcttgcg
     2161 aaaaagagca tggtggtggt gcccaaccat ctggttgagc aatggggtaa agcgtttctc
     2221 cagctttacc cgcaagccaa catctttgtc gccggtaagg agcattttca gggcgacaag
     2281 cgggcgaagg cgatgtcgcg cattgcgacc ggcactttcg acgcggtgat cgtgtcgcac
     2341 aaaagctttg aacttctgcc ggtctcggat gccttgttcg acggctatat gcaggaggag
     2401 ctggaggcgc tcgaaaaagc catccgtgag gcgagtgcgg ataaaagcag ccgccgcatc
     2461 gtcaaaatcc tcgaagcgga caagaaacgg ctcgtcaaga gaatcgagga tagggccaag
     2521 cgcgagaaga aagacaagac tgtcaccttc gaggaactcg gcattaccaa catttttgtg
     2581 gacgaagccg atctctataa aaatctcggc ttcactacca aaatggcgcg cgtggcggga
     2641 ttgccgaact ccaacagcaa ccgcgccgtc gacatgcaca tcaagacccg ttacctgcgc
     2701 aagcagaata acggcaaggg cgtcgtgttc gctaccggca cgccgatatc gaacacaatg
     2761 gcagaacttt acaccatgaa gcggtacttg gcgccggaag ccctggaagc cgctggcatg
     2821 gctgctttcg acgcctgggc cgcgaatttc ggggaaacgg ttacaagttt ggaggtgaca
     2881 ccggacggcg gtggttaccg gatgaaaaca cgcttcgcca aattcgtgaa tttgccggaa
     2941 ctccttacga tgttccgcga ttttgccgat gtgcagacag cggacatgct caatcttccg
     3001 cggccggcgc tcaaaggtgg gcagaacaag gttgttgtgg cgccttcgac gccggagctc
     3061 aaaacctatg tcgctagcct tgtgaaacgc gccgaccgga ttaaaaccgg cagcgtggat
     3121 ccgcgcgacg acaacatgct gaagatcacc agcgacggac gcaaggcggc gctcgatatg
     3181 cgcctggtgg acccgtttgc caccgtccat ccggaaagca aggtcaatct ggtgatcaac
     3241 gaagtattca ctatttggaa ggaaaccgaa cagcaacggg ctacccaact ggttttctgc
     3301 gatttgtcga cgcccagccc cgatcgtttc catgtgtacg gtgccatccg ctcaggattg
     3361 atcgagcgcg gcgttcccgt gagcgaaatc gatttcatcc atgacgccga caccgacgcg
     3421 gccaagcagg cgttgttcga gaaggtgaac agtgggcgcg ggcgcattct gctcggctcg
     3481 accgaaaaaa tgggcgctgg gacaaatgtt cagaggtttc tttatgcctc gcataatgta
     3541 gacgcaccat ggcggccgcg cgacatcgag cagcgcgacg ggcgcatttt gcgccccggc
     3601 aacactcacc caggagtgct gatttttcgc tttgtcaccg agggctcgtt cgatgcctat
     3661 atgtggcaaa ctctggaagc caaggcccgt ttcattcagc aggtgatgaa tggcgagacc
     3721 tcggtccgca cagccgagga catcaccggg ggtgccctca cctatgccga gatcaaggct
     3781 atagcatccg gcaatcccgc cgtgatggaa aaggtgaagg ttgatacgga agtgcgcaag
     3841 ctggattcgc tgcgcacgtc gcacgcgaaa cgcaaattcg agatacggcg caaaatcggt
     3901 gttacgcaaa cgaggatcgc caggaacgag gagcagctag ccatggtcgg taaggacatt
     3961 gccacccgcg aggccaacat gggcgagcaa ttcgccatga cgctcgcggg ccggcgctac
     4021 accggcaagg agggccgcga acaggccgcg aaggaattaa acggcgttat cctcaccctt
     4081 agcggcgatg cggaagcaaa atgccgcggc cagatcgcgg gcttcgatat gatgacgcgg
     4141 gcgacgcccg atcgcgaggt aaccgcctat ctgcgcggct ttgctgcgta tacatttcat
     4201 cacaatctcg aaaatccgct tggcacgatc atgagcatcg agcgcacgct gcgcggtttt
     4261 gaagccatcc aggggcggtt ggagagcgac atcgcgcgcg atcaaaagga gctcgctgaa
     4321 tatcgggtgg aaagcgagaa gccgttcgaa tatgaagaac agctgaagca attgctcgtc
     4381 aagcaagcgc aactgaatgc cgagcttgat ctcggcaagg acgataaaca ggcggtaaca
     4441 gccgaaagtg agggaggcgc cgccaaagct gtcgaggggc ttgttgcggt tgtcgacggt
     4501 cttatgggcg gcgggcagaa atccacgaaa cagcaggcgc acgacaaggc gcaagcggcc
     4561 ggcaatgtcg agacgcgcga cgccgaagcc tatgcagcca aagacgtagc gctgatcgac
     4621 caagctagaa ttccggcgga actggagcgc caggtcttgg aggatcagcc cgttgaaatc
     4681 ttcagggcgg catcagacgg cgacaaaata cacagttatt tgcagagtct ccagcaacag
     4741 cagtccgtat ctgcagagct acaggccgac agatccgaga tcatcaagga gtatgatcgc
     4801 atcgtcgacg gccggcccga agcgcgaaaa gagcttgagc aaaccaaccc ggccctggtt
     4861 gctgcccgtg acaaagccgt aaccgagctc cggcgcgaaa
//
LOCUS       DATMPU010000102         3675 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_1625031,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000102 DATMPU010000000
VERSION     DATMPU010000102.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 3675)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3675)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3675
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1625031"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            484..2064
                     /locus_tag="VNY06_02320"
     CDS             484..2064
                     /locus_tag="VNY06_02320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020176722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HWX13705.1"
                     /translation="MRANHSESDIAGVVVDGNTGKTLYARNENEQRFPASITKVMTLY
                     LLFEQLEQGRLRLDSDIRVSAFAAAQKPTKLGLHPGGSIRVDDAIRAVVTRSANDIAV
                     AIAETIGGDESHFATLMTQKARELGMTRTHFANASGLPNADQFTTAHDLAILGRAVQE
                     RFPRYYGYFATRVFYFRHAAIVNHNHLLDRVEGMDGIKTGYTNASGFNLLTSVKRGGH
                     YIVAVVLGGPSARSRDRIIAGLIEHHIEDGATVRAASLLTGTNAMEAAKALQGRTSSP
                     GSIESPSASALRPAFTPGAPRPRAAVDPIQVASINADNVPPYRPRPAFISGAPKPAGP
                     NDRALKAEPSWKQKHLDGSTARGLPEDRAGPSTATPSALREGSSDAQHYPAKFANRIS
                     EPSQIATVERVQQKPEPVLRPDTRQSKAVEQDDDSKIGHLALSTTEVPRVAEERDGWM
                     IQIGATPNVDKATELLARARSEGPKTLARARAFTEKIQKGSETLYRARFAGLEEDTAA
                     FVCKSLKRAGFSCFATRN"
     gene            complement(2117..2836)
                     /locus_tag="VNY06_02325"
     CDS             complement(2117..2836)
                     /locus_tag="VNY06_02325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015927107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HWX13706.1"
                     /translation="MIYLLAGLLLFWLMTVALKAFATANPAMLARIICRGSGSVAILA
                     GTILLFRGRIDMALGLGGLGVWLLGLRGGPLSRILRASGRSRRANVSCVRSAMIEMEL
                     DHDTGKMRGTVLAGPDEGKSLEAMTRPQCEALYNLCCADDPDGARLMEAYLDRRFAGW
                     RSAAQNQNDSRRGQARSRFGMMSEDEAYEVLGLHKGASEEEVVRSHRALMKKLHPDHG
                     GSTNLAARVNEAKDVLMRRHQ"
     gene            complement(2851..3570)
                     /locus_tag="VNY06_02330"
     CDS             complement(2851..3570)
                     /locus_tag="VNY06_02330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015685559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HWX13707.1"
                     /translation="MGRPEQKGGAIAPRSSTKLDIDAFLDAAARLPTAADTGRGRLVF
                     ALDATMSRQATWDIAQSVQGRMFVTAAAHGGLEVQLVYYRGLSECKASGFVNGGQRLA
                     SLMTKIAVGAGQTQIEKVLRHVIDETHKGPVRALVFVGDAMEEQLDALVGLAAELGLL
                     GVKAFLFQEGRDRVAEQAFRKIAMLTGGAYATFDLSAPDRLVALLSAAAAYAAGGRRA
                     LEFEARAAGTGAADLLLSQLR"
BASE COUNT          853 a         1154 c          951 g          717 t
ORIGIN      
        1 atgtcatcga ttttatctat catttgaagt tcctttaccg ttggcgcccc agtcctcaag
       61 taaccgaagc atcaagacac cgaggccgtt cgcctcatga ttgtcatata gtgcgttgca
      121 caaatcacgt caagttgatg ctgcagcgca ccgtgatgcg cggatacagc ttcgcacagg
      181 ctacgagtcc caggcttaac taagcggtaa ccgcaacaga ctaacgttct gaaagcaagc
      241 agccggctgc tgacatagaa tctgccctgc aggggaccgg aaatattgca aatgttgaac
      301 ctttgcgaga catttagccg caatctaagg cccgcggcgt gcatttgcct ggttatagct
      361 acagcaagtt ttagcccctg ggatgccgaa gcaaggcatt ggcgcgcgca ccaccggcac
      421 gccgcggccc attatcattt tgcgcactgg cgcgatcgtc acgcttcaat tcgctacggc
      481 aatgtgcgcg caaatcacag cgaatcggac atcgcaggtg ttgtagtcga cggcaatacc
      541 ggtaagacac tttacgcccg taacgaaaac gaacagcggt ttcccgcctc gatcaccaaa
      601 gtgatgacgc tctatctctt attcgaacag ctcgaacagg ggcgtttgcg actcgacagc
      661 gacattcgag tctcagcctt tgcggcggca caaaagccaa ccaaattagg gctccaccca
      721 ggtgggtcga tccgcgtgga cgacgcgatt agagcagttg ttacacggtc ggcaaacgac
      781 atcgcagtcg ccatcgccga aaccatcgga ggagacgaat cgcactttgc aactctcatg
      841 acgcaaaagg cgcgtgaact tggcatgacc cgcacgcatt tcgccaacgc ctcgggtctg
      901 cccaatgccg atcagtttac gacggcacat gaccttgcaa tactcggccg cgccgttcag
      961 gagcgttttc cgcgatacta tggctatttc gcaacgcggg tgttctattt tcgccacgcc
     1021 gctatcgtga accacaatca tctcctcgat cgtgtcgagg gtatggacgg tatcaagacc
     1081 ggttatacca atgcttccgg attcaatctc ttaacttcgg taaagcgcgg cgggcactat
     1141 atcgtcgcgg tggtgcttgg cggcccaagc gcacggagcc gcgatcgcat catagcagga
     1201 ttgatcgaac atcacatcga ggatggcgcc accgttcgtg cagcctcgtt gctgactggc
     1261 acgaacgcca tggaagccgc aaaagccttg caaggccgca cgagcagccc aggctctatc
     1321 gaatcgccgt cagctagcgc cctcagaccc gcatttacgc ccggcgctcc tagaccccgg
     1381 gctgccgttg atccaattca ggttgcttcg ataaatgccg ataatgtacc gccctataga
     1441 ccaagacccg catttatttc cggagcaccc aagccggcag gcccgaatga tcgcgcactc
     1501 aaggccgagc ccagttggaa gcagaagcac ctagacgggt cgaccgcgcg cggccttccc
     1561 gaagatcgcg ccggcccttc gacggcaacc ccatccgccc ttcgtgaagg ctcaagcgac
     1621 gcgcaacatt accctgccaa attcgcaaac cgaattagcg agccctcgca aatcgccacc
     1681 gtagagcgcg ttcagcaaaa gccagaacca gttttgcgtc cggacacacg acaaagcaaa
     1741 gcagtagagc aagatgacga ttcgaagata ggtcaccttg ccctatcaac caccgaggtc
     1801 ccccgcgtgg ctgaggagcg cgatggatgg atgatccaga tcggcgcaac cccaaacgtg
     1861 gacaaagcaa ccgaacttct tgcccgcgcc agatccgaag gtcctaaaac tttggccagg
     1921 gcacgcgcct ttaccgaaaa aattcaaaaa ggttcagaga ctctctaccg cgcgcgcttt
     1981 gcaggtctcg aggaggatac cgcggcattc gtctgcaaat ccctcaaacg tgcagggttc
     2041 tcctgttttg caacaagaaa ttgaatgatt tgccaaaacg caacgctgga tttcagttta
     2101 acctcaaaac gcagacctat tgatgacgcc gcatcagcac atccttagct tcgttgactc
     2161 gagccgccag attcgtcgat cccccgtgat cgggatgcag cttcttcatc aacgcgcggt
     2221 gcgaccgaac gacttcctct tccgaagcgc ccttgtgaag tcccaagact tcatacgcct
     2281 catcttcgga catcatgccg aagcggcttc gcgcttgtcc gcgccgagag tcgttttggt
     2341 tttgcgctgc cgaacgccat ccggcaaacc ggcggtcaag ataggcttcc ataaggcgcg
     2401 ccccgtcggg atcatccgca cagcagaggt tgtagagggc ttcgcattga ggtctggtca
     2461 ttgcttccag tgattttccc tcgtcgggcc cagccagcac tgttcctcgc attttgccag
     2521 tatcgtgatc aagctccatc tcgatcattg ccgacctaac gcaggaaaca ttcgctctcc
     2581 ttgaacggcc tgaggcgcga aggatccgcg acagcggccc accgcgaagc ccaagcaacc
     2641 acacgcccaa tccaccaagc ccaagcgcca tatcaatgcg tccccggaac aataatatcg
     2701 ttcccgcaag aatcgccaca ctgccactgc cgcggcagat gatgcgagcg agcatcgcgg
     2761 gattggccgt cgcgaaggcc ttgagcgcga cggtcatgag ccaaaacagc aaaagtccgg
     2821 cgagtaaata gatcattcta tgccgccagt ttagcgcaat tgcgacaaca gaaggtcggc
     2881 tgctccagtt cccgccgcgc gggcctcgaa ctcgagggcg cggcgacctc cagcggcata
     2941 ggccgccgcc gccgagagca acgcaacaag cctgtccggg gcggacaagt cgaaggttgc
     3001 gtaggcgccc ccggtcaaca tggcaatctt ccgaaaagcc tgctccgcta ctctatcgcg
     3061 cccttcttgg aacaagaaag cctttacgcc caaaagcccc aattccgcag cgagccccac
     3121 caaagcatcc agctgctcct ccatggcatc cccgacgaaa accagggcgc ggacggggcc
     3181 cttgtgcgtt tcatcgatca catgccgcag cactttctcg atctgggtct ggccggcacc
     3241 gactgcgatc ttcgtcataa gcgaggctaa acgctggccg ccgttcacaa agcccgatgc
     3301 cttgcactca gaaaggccac ggtaatagac aagttggacc tcgagaccgc catgggctgc
     3361 agcagtcaca aacatgcggc cttgaacgct ttgcgcgatg tcccaggttg cctggcgact
     3421 catcgtggca tcaagcgcga agacgagccg cccccttccc gtatcagccg cagtcggcaa
     3481 tcttgccgcc gcgtcgagaa acgcatcgat gtccaacttg gtactggacc taggcgcgat
     3541 agcgccaccc ttttgttctg gtcgccccac ttagctccct cgagaatttc attttctagg
     3601 aatttataga tcgtgctagc acggcgcaac ggcaagcgtc tctcccccgg aaaggctagg
     3661 gaagctcgct tgagc
//
LOCUS       DATMPU010000103         6943 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_1010506,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000103 DATMPU010000000
VERSION     DATMPU010000103.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 6943)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6943)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6943
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1010506"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..304
                     /locus_tag="VNY06_02335"
     CDS             <1..304
                     /locus_tag="VNY06_02335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome c family protein"
                     /protein_id="HWX13708.1"
                     /translation="KKAGRTSNRANPAIISRRVPGPSQVGRAKGSFQGFEYSEALKSK
                     GGNWTYADLDLFLANPKTFAQGTKMAFAGESDPAKRADIIDYLHSLSDNPEPLPAN"
     gene            489..2405
                     /locus_tag="VNY06_02340"
     CDS             489..2405
                     /locus_tag="VNY06_02340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020174152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HWX13709.1"
                     /translation="MLGRKIVTASHFKRLSIALTRRSALKLGIAGLVLPRVFLAGARA
                     AAAETETHGLSIFGDLALPADFTHFPYVDPDAPKGGEISLQTSSTSGNQNFTTFNTLN
                     IFILKGDGAAGMGLIFDSLMAGSGDEPDSLYGLIARAVRISADKNTYRFLLRREARFH
                     DGSPLTAGDVAFSLNILKTKGHPSIRQPLRDLDAAEAEADDILLVRLKPQHSREAALI
                     VAGQPIFSAAYYKNHSFDETTLEPPLGSGAYKVGDFDTGHYIAFGRVPDYWGRNLPVN
                     IGQANFDRIRFEYFGDRKVAFEAFKAGVFTFREEFTSAVWATGYDFPAIKEGKVLRAT
                     LPDESPMGTQGWFLNIRRDKFKDPRIRQAIGLAFDFEWTNANIMYGVYSRTTSFFQNS
                     PMAAQGSPAPQELALLEPFRKDLDPFVFGEVYVPPVSDGSGQDRELLRQAAKLLSDAN
                     CTRKGTALVLPDGKTFEIEFLDFDNALQPHTAPFIKNLRLLGIEARYRMVDAAQYKRR
                     LDDFDYDIVTSRFGLGLTPGEGMRSAFGSEAANLPGARNVSGIANKAVDSLIEKALVA
                     ATREELTFICRAIDRILRCGHYWVPMWNKPNHLVAYWDLFGRPERGPRYDIGVVSTWW
                     YNEEKAKQIHLSGR"
     gene            2416..3504
                     /locus_tag="VNY06_02345"
     CDS             2416..3504
                     /locus_tag="VNY06_02345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016919262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter permease YejB"
                     /protein_id="HWX13710.1"
                     /translation="MLAYIARRLLFMVPTLFGILFINFVIVQFAPGGPVERILAQLQG
                     VESGATARFGGGGDIGNTGGADLSSKYRGAQGLDPKFIAELEKQFGFDRPAYERFFIM
                     LKNYATFDFGRSYFRDVPVWQLIKEKLPVSISLGLWMTLLSYSISIPLGIKKAVSDGS
                     RFDIWSSAVVITGYAIPSFLFAILLIVLFCGGSFWQIFPLRGLTSENWSALSLSGKIT
                     DYFWHITLPVISMTLGAFATSTFLTKNSFLDEIGKQYVQTARMKGLSERAVLYGHVFR
                     NAMMIVVAGFPGAFIAAFFGASLLIETIFSLDGLGLLSFESIVNRDYPVVFADLYIFA
                     LLGLVVNLLSDLTYMWIDPRIDFETREV"
     gene            3507..4676
                     /locus_tag="VNY06_02350"
     CDS             3507..4676
                     /locus_tag="VNY06_02350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009494576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWX13711.1"
                     /translation="MRASYSNGTAVTAPAPARARRQWFQLSPINRRRFANFRANKRGY
                     WSFWIFLFLFTVSLFSEFVANDRPLLVVYRGEILFPILRDYPEEKFGGFLAQTDYRDA
                     VIAKEIREHGFMIWPPIRYSYKTINRQLPVPAPSPPTWMLTEAQCGTAVLRYDKKQNG
                     TCDSIEWNWLGTDDQGRDVLARLLYGFRLSILFGLTLAGVSSIVGITAGAVQGYFGGW
                     TDLIFQRLIEIWSSLPHLYLLIIISSIITPSFFVLLGILLLFSWVSLVHVVRAECLRT
                     RNFEYVNAARALGLSNMRIILRHVLPNATVATLTFLPFVLNGSVTTLTALDFLGFGLP
                     PGSPSLGELLLQGKSNLAAPWLGITGFVVIALMLSLLVFIGEAVRDAVDPRKTLS"
     gene            4737..6359
                     /locus_tag="VNY06_02355"
     CDS             4737..6359
                     /locus_tag="VNY06_02355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018265733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWX13712.1"
                     /translation="MTNTLLEVRNLNVHFRQAGVTTSAVDGVSFALARGRTLGLVGES
                     GSGKSVTALSILRLLGRAALVQGKVLFKGEDISACSESRLRDIRGAGITMVFQEPMTS
                     LNPLHTIERQVGEILELHGARGAQKIRSRIVELLEEVGIPKPAERLSAFPHQLSGGQR
                     QRVMIAMALANRPDLFIADEPTTALDVTVQAQILELLKRLQARHNMAMLFITHDLGIV
                     RKIADDVAVMQKGKIVETGPVVEIFSSPSHPYTKALIEAEPKGAAPAINLAAKTILEG
                     KDVRVWFPIKRGFFRRTVGFVKAVDGVSVTVREGETVGVVGESGSGKTTLGLALLRLI
                     RSKGAIVYLGRRIDGLNFKAMRPLRRDMQIVFQDPYGSLSPRLPVADIVAEGLTVQYK
                     RLSIQRRREIVAEALKDTGLDPGAMDRYPHEFSGGQRQRIAVARAIVLEPNFIVLDEP
                     TSALDMSVQAQIVDLLRDLQKRRGLAYLFISHDLKVVRALASEIIVMRDGKVLESGPA
                     SELLANPQSAYTRALFAAAFDIKAENGAGLEQ"
     gene            6337..6906
                     /locus_tag="VNY06_02360"
     CDS             6337..6906
                     /locus_tag="VNY06_02360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX13713.1"
                     /translation="MARAWSNDSNAQKSGLEVSAAFASAKAAQRSLAQSMARGLGPSG
                     IHVALAVIDGVIDSPRARSQLADKPDTFFLAPDAVAETVYCLTKYGLLPRTGELTSPS
                     RFLFGNGEGLRAGRQAIGQIERAQRSKKIRSVAARPRLRFSGNRFLIIIRRWGTHGRY
                     HKSALSRVLMHLDLRLARWAIAHHQIPID"
BASE COUNT         1366 a         2023 c         1880 g         1674 t
ORIGIN      
        1 aaaaaaggcc gggcggacgt caaaccgtgc caatcctgcc ataatttcga gaagggtgcc
       61 gggcccaagt caggtcggcc gcgcaaaggg ctcgttccaa ggcttcgaat attccgaagc
      121 cctcaaatcc aaaggtggca actggaccta cgccgatctc gacctctttc tggccaatcc
      181 gaagaccttt gcccaaggca cgaaaatggc ctttgcgggc gagagcgatc ctgcaaagcg
      241 cgccgacatc atagattacc tgcacagtct atcggataat cccgagcccc tgccggcaaa
      301 ttaggctttg ggagctgtga ttttgcggct tcggattctc tgcccagccg ttttatgttt
      361 atatagccta tttgtatagc ctatcgccca cccaccgctc ggtttaagcg agatttccga
      421 aaaattgccg tcttattgga taaaagcaaa gagatagaag cgggcggcga tcctcgtaaa
      481 gaaccgtcat gcttgggaga aaaatcgtga ccgcgagcca ttttaagcga ttgtccatcg
      541 cgctgacgcg ccgctcggcg ctgaagcttg gcatagcggg cttggttctg ccgcgcgttt
      601 tcctggccgg agcgcgggca gccgcagctg agaccgagac gcatggtctg tcgatcttcg
      661 gcgatcttgc cctgccagcg gatttcaccc actttcctta cgtcgatcca gatgccccca
      721 agggcggcga gatttccctg caaaccagct cgacctcggg caatcagaat tttaccacct
      781 tcaataccct aaatatcttt atattgaagg gggatggggc cgccggaatg gggctcattt
      841 ttgattcgct gatggccgga agcggcgatg aaccggattc gctttacggc cttatcgcgc
      901 gtgcagtgag aatatcggcc gacaagaaca cctaccgttt tcttctgcga agggaagcaa
      961 gatttcacga tggctcgcct ttgaccgccg gcgatgtcgc cttttcccta aatattctga
     1021 aaacaaaggg ccatccttcg atccgccagc ctctacgcga tcttgatgcg gcggaggcgg
     1081 aggcggacga catcctgctt gtccgcctaa aaccgcagca tagccgggaa gcggcactga
     1141 tcgttgctgg acagccgatt ttttctgccg cctattacaa gaaccattct ttcgatgaaa
     1201 cgacattgga gccgccgctc gggtcaggcg cctacaaggt cggcgatttc gatacggggc
     1261 actatatcgc tttcggtcgc gtgccagatt attggggcag aaatcttccc gttaacatcg
     1321 gccaagcaaa tttcgatcgc atccggtttg aatattttgg cgaccgaaaa gtcgctttcg
     1381 aggcttttaa agcgggcgta tttacctttc gcgaggaatt cacctcggct gtgtgggcta
     1441 cgggctatga cttccctgcc attaaggagg ggaaggtttt acgcgcgact ctgcccgatg
     1501 aatcgccgat gggcacgcag ggctggttct tgaacataag gcgcgacaag ttcaaggacc
     1561 caagaatccg gcaggcaatc ggtcttgcct tcgatttcga atggaccaac gccaatatca
     1621 tgtatggcgt ctattcgcgc acgacgtcct ttttccagaa ttcaccaatg gccgcgcaag
     1681 ggagccccgc tccgcaagaa ctcgcgctgc tcgagccgtt ccgcaaggac cttgaccctt
     1741 ttgtattcgg cgaggtctat gtcccacctg tgtccgatgg ttcgggacag gaccgcgagc
     1801 ttttgcggca agcagcaaaa ctcctctccg atgcaaactg cacgcgcaag ggcacggcct
     1861 tggtcctgcc agacggaaaa acctttgaga tcgagtttct ggattttgac aatgctctgc
     1921 agccgcacac agcgcccttc atcaagaatt tgagactgct tggcatagaa gcgcgctacc
     1981 ggatggtcga tgcggcccaa tataagcgcc gccttgatga ttttgactat gacatcgtga
     2041 cctcgcgctt tggccttggt ctcacgccag gggaaggcat gcggtccgca ttcgggtcgg
     2101 aagcggcgaa ccttcccggc gcgcgcaacg tttcggggat cgccaacaaa gccgttgata
     2161 gtttgatcga aaaagctttg gtcgccgcaa ctcgggaaga actgactttt atctgccgcg
     2221 ccatcgaccg tatcctgcgc tgtggccatt attgggttcc gatgtggaac aagcccaatc
     2281 atctcgtcgc ttattgggat ctgtttggcc ggccggaacg cggaccgaga tatgatatcg
     2341 gcgtcgtttc gacctggtgg tacaacgagg aaaaagccaa gcagatacat ctctccggcc
     2401 gttgaagggc gtttgatgct agcctatatc gcgcgccgcc ttttgttcat ggttccgacg
     2461 cttttcggaa tcttgttcat caactttgtc atcgttcaat tcgcgccggg cgggcccgtc
     2521 gagcgcatat tggcgcaatt gcagggcgtc gaatcgggag cgaccgcccg cttcggcggc
     2581 ggcggcgaca tcggaaacac cggcggcgcg gatctctcct ccaaataccg tggcgcgcag
     2641 gggcttgatc cgaaattcat cgccgagctc gaaaagcaat tcggattcga caggccggcc
     2701 tatgagcgct ttttcattat gctcaagaat tatgcgacat tcgattttgg ccggtcttat
     2761 tttcgcgacg tgccggtctg gcagctcatc aaggagaaac tgccggtatc catctcgctc
     2821 ggactttgga tgacgctcct ttcctattcg atatcaattc cgctcggcat caagaaagcg
     2881 gtcagcgacg gctcgcggtt cgacatctgg tcttcggccg ttgttatcac cggctatgcg
     2941 atcccaagtt ttctcttcgc aattcttctc attgtccttt tttgtggcgg ttcgttttgg
     3001 cagattttcc cgctgcgcgg cttaacctcg gagaattggt ccgcgctttc tctttctggc
     3061 aagatcacag actatttttg gcacatcacc ctgcctgtta tctcgatgac tctcggcgct
     3121 ttcgcgactt cgactttcct aaccaaaaac tcgtttcttg atgaaatcgg caagcaatat
     3181 gttcagaccg cgcggatgaa gggactgagc gagcgggccg tgctctatgg ccatgtgttc
     3241 cgcaacgcca tgatgattgt cgttgcgggt tttcccggcg ctttcatcgc ggcatttttc
     3301 ggcgcctctc tcttgatcga aaccattttc tcactcgatg ggcttggcct gctttcgttc
     3361 gaatcgatcg tcaatcgaga ttatccggtc gttttcgccg acctttatat ttttgcgctg
     3421 cttggccttg tcgtgaatct cctttcagat ctaacctata tgtggatcga tccacgcatc
     3481 gattttgaaa cgcgggaggt ttagcaatgc gcgcctccta ctcgaatggg accgcggtaa
     3541 ctgctcccgc accggcccgt gcgcgccgcc agtggtttca gctaagcccg atcaatcggc
     3601 gccgctttgc caactttagg gccaacaagc gcggctactg gtctttctgg attttccttt
     3661 tcctttttac cgtttcgctc ttttccgaat tcgtcgccaa cgacaggcca ctcctcgttg
     3721 tctatagagg cgagattctg tttccgatcc tgcgcgacta cccggaagaa aaatttggcg
     3781 gctttctggc acagaccgat taccgtgacg cggtgatcgc aaaggagatc cgtgaacatg
     3841 ggtttatgat ttggcctccg atccgctatt cctacaaaac gatcaaccgc caattgcccg
     3901 tgcccgcgcc ctcgcccccg acctggatgc tgaccgaagc ccaatgcggc acggctgtct
     3961 tgcgttacga caaaaagcaa aatggcactt gtgacagcat tgagtggaat tggcttggga
     4021 ccgacgatca gggccgcgac gttctggcga ggctgctcta tggttttcgt ctatcgatcc
     4081 tgttcgggct cacgctcgcg ggagtttcct caatcgttgg aatcaccgcg ggggcggtgc
     4141 aaggctattt tggcggctgg accgatctta tcttccagcg tcttatcgaa atttggtcct
     4201 cgctccctca cctttacctc ttgatcatta tctcctctat catcacgccg agcttttttg
     4261 tactgcttgg catacttttg ctgttttcct gggtctcgct tgttcatgtc gtgcgcgccg
     4321 aatgtctgcg cacgcgcaac ttcgaatatg tcaacgccgc gcgcgcgctt gggctttcca
     4381 atatgcgcat catccttcgc catgtgcttc ccaatgcgac ggttgcaacc ttgacctttc
     4441 tgccctttgt gctgaacggc tctgtcacga ctttaactgc tctcgatttt ctgggctttg
     4501 gcttgccgcc cggttctcct tcccttggcg agcttttgtt gcaaggaaaa tccaatctag
     4561 ccgcgccctg gcttggcatc accggttttg tggtcatcgc gctcatgctc tcccttctcg
     4621 tctttatcgg cgaagccgtg cgcgatgccg ttgatccgag gaagacactt tcatagcggc
     4681 gagtagtccc tttcacgcat cgcaaaaatc ctctatttgg tcttacgaga ctgtttgtga
     4741 ccaacactct ccttgaagtc cgcaatctca acgtccactt ccgccaagcc ggcgtgacca
     4801 catctgccgt cgacggcgtg tccttcgcgc ttgcaagagg gcgcactctg ggacttgttg
     4861 gcgaatcggg ctccggcaaa tccgtcaccg ccctttctat cctgcgcttg cttggacgcg
     4921 ccgctttggt tcaaggtaag gtgctgttca agggcgagga catctccgcc tgctccgagt
     4981 cgcggctgcg cgacattcgt ggcgcgggga tcactatggt gtttcaagag ccgatgacct
     5041 cgctcaatcc gctccatacg atcgagcggc aggtcggcga aattcttgaa ctccatggcg
     5101 cgcgcggagc gcaaaaaatc aggagccgca tcgtcgagct cttggaggag gtcggcattc
     5161 ccaagcctgc cgagcggcta tccgccttcc cgcatcagct ctcgggcggc cagcgccagc
     5221 gcgtgatgat cgccatggcg ctcgccaaca ggccagacct cttcatcgcc gatgaaccaa
     5281 caaccgcact tgatgttacg gtgcaagcgc aaatcttgga gcttttgaag cggcttcagg
     5341 cccgccataa catggctatg ctctttatta cccacgacct cggaatcgtg cgtaagatcg
     5401 ccgacgatgt ggccgtgatg cagaagggca agattgtcga aacagggccg gtcgtcgaga
     5461 tttttagctc cccaagccat ccctacacaa aggcgctgat cgaagccgag cccaaaggcg
     5521 cggccccggc gatcaatctt gccgccaaga ccattctgga aggcaaagat gtccgcgttt
     5581 ggtttccgat aaaacgcggg tttttccgcc ggacagttgg cttcgtcaaa gccgtcgatg
     5641 gcgtctctgt gactgtgcgc gaaggcgaga cagtcggcgt tgtcggcgaa tcgggttctg
     5701 gtaaaaccac gctcggcctt gctcttcttc gtctcatacg ttcaaaaggc gcaatcgtct
     5761 atctcgggcg ccgcatcgat gggctcaatt tcaaggcgat gcggccgctc cggcgcgaca
     5821 tgcagatcgt gtttcaagat ccctacggct ctctgtcgcc ccgcctgcct gtggccgata
     5881 tcgtcgccga aggcctcacc gtacaatata agcgcctctc gattcaacgc cggcgcgaga
     5941 tcgtggcaga agcgctcaaa gacaccggcc tcgaccccgg agccatggat cgctatccac
     6001 atgaattttc gggcggtcag aggcagcgca tcgcggtcgc gcgcgccatt gtgcttgaac
     6061 ccaattttat tgtgctggat gagccgacgt cggcgctcga tatgtcagtg caagcacaga
     6121 tcgttgatct tttgcgcgac ttacaaaagc ggcgcggact tgcttatttg ttcatcagcc
     6181 acgatcttaa ggtggtccgg gcgctcgcaa gcgaaatcat cgtcatgcgc gacggcaagg
     6241 ttctcgagtc tggcccggcc tcagagcttc tcgccaaccc gcagagcgcc tacacgcgcg
     6301 cgctttttgc ggcggccttc gacatcaagg ccgagaatgg cgcgggcttg gagcaatgat
     6361 tccaatgcgc aaaaatctgg acttgaggtt tcggccgcct tcgcctcggc gaaagccgcg
     6421 caacgcagcc tcgcccagtc gatggcgcgc ggccttgggc cttcgggaat ccatgtggcg
     6481 ctcgcggtca tcgacggcgt cattgattcg ccgcgagcgc gcagccagct tgcagataag
     6541 cccgacacat tcttcctagc gcccgacgcc gtcgccgaaa ccgtttattg cctgacgaag
     6601 tacggccttt tgccgagaac tggcgagctt acttcgccgt cgcgtttcct attcggcaac
     6661 ggagaaggct tgcgggctgg gcgtcaggcg attggccaaa tcgaacgagc gcagcgcagc
     6721 aaaaagatca ggagcgtagc ggcgaggccg cgtcttcgat tctcggggaa ccgttttttg
     6781 attatcatcc gccgttgggg gacgcatggt cgttaccaca aatcggcact ttcccgagtg
     6841 ctaatgcatc tcgacctacg cctggcacgc tgggcgattg cacaccatca aataccgatt
     6901 gactgacgaa aggttgcgtc ggcgccgaac caagctcgaa att
//
LOCUS       DATMPU010000104         8482 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_384291,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000104 DATMPU010000000
VERSION     DATMPU010000104.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 8482)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8482)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8482
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_384291"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            320..940
                     /locus_tag="VNY06_02365"
     CDS             320..940
                     /locus_tag="VNY06_02365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020176590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HWX13714.1"
                     /translation="MANPRAWVRLLSGKRLDLLNPTPFDWEDEDLALGLARTYRWGGH
                     SAWPLPLSVAQHSLLVLALHRQRHKDTAIAPAELRELLHDADEGLLGFDCISVVKPFL
                     GEAYRLLTERLANAIALRYRLPPWTSADRKAHRQADWVAAASEAVHVVGWTQSEVRNT
                     LRIPYQALSTDPLCTIYGGKAWEPWAPPVATERFLVELKRLVSPQS"
     gene            1079..1591
                     /locus_tag="VNY06_02370"
     CDS             1079..1591
                     /locus_tag="VNY06_02370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012320921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-tyrosine phosphatase family protein"
                     /protein_id="HWX13715.1"
                     /translation="MPRIHVCPLSRLPHTAEASGARSLVTLIDEGTPVDRPTGIAAEK
                     HLALILSDISVETFGHTLPNDSHVEKLLAFVRHWDQAAPLLIHCFAGVSRSTAAAFIA
                     ACALLPHRDEAEIALAVRKNSPTATPNPRLVAIADKLLGRKQRMTSAVARIGRGADCS
                     EGIPFALELR"
     gene            1668..1916
                     /locus_tag="VNY06_02375"
     CDS             1668..1916
                     /locus_tag="VNY06_02375"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020176593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HWX13716.1"
                     /translation="MTATDTDIAAKSFEDALKELEQIVDRLEKGTVGLEESIAIYARG
                     EALKSHCNALLHNAEQRIEKISLGPDGRPAGTEPLDVE"
     gene            1916..2683
                     /locus_tag="VNY06_02380"
     CDS             1916..2683
                     /locus_tag="VNY06_02380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012384169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HWX13717.1"
                     /translation="MPGSLVAKPNSLANGLRLAPARQRMDSMAEYAFPPPPLITLPAP
                     PGKPFPVRRIFCVGQNYRDHAKEMGGNPDTDPPFFFMKPASAIVQNRTTIPYPPGSRD
                     LQHEAELVVALQAGGRNIPVEKANDMIFGYAVGLDMTKRDVQAEFREKGRPWEMAKAF
                     DFSAPCSAIALKIQTGAIAKGKIECMVNGELRQSGDLSHMIWGVPEIVAYLSQQVEIA
                     SGDLIFTGTPSGVGPVTKGDRIEARIAGLEPLVIEIG"
     gene            complement(2695..3099)
                     /gene="mce"
                     /locus_tag="VNY06_02385"
     CDS             complement(2695..3099)
                     /gene="mce"
                     /locus_tag="VNY06_02385"
                     /EC_number="5.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013421170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA epimerase"
                     /protein_id="HWX13718.1"
                     /translation="MIGRLNHIAIAVKDLEAASALYRGALGAKVSNPTALPDHRVRVV
                     FVKLPNTRIELIEPLGDHSPITSFLAHHPGGAIHHICFEVEDILAAREQLIGAGTHVL
                     GDGIPKIGAHGKAVLFLDPKDCAGTLIELEQR"
     gene            complement(3121..4791)
                     /locus_tag="VNY06_02390"
     CDS             complement(3121..4791)
                     /locus_tag="VNY06_02390"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018391207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HWX13719.1"
                     /translation="MNDGYDELVFVPLGGLGEIGMNLALYGYGRKGSWKWLVVDCGVA
                     FAGPDLAGIDIVIPDISFIEKMRGDLAGLVITHAHEDHIGALTEIWPRLGCKLYATSF
                     AAALFGAKRVGEPGSPDVQFSIVAQGEVKVIGPFNIEFVGMAHSIPESCALAIRTPLG
                     TILHSGDWKIDPSPGIGKVTDAKRLAAIGAEGVLALVSDSTNILRDGLSPSEADVAKS
                     LRDIIGAASGRVIVTIFASNVARIRAVAEAAMAAGRAVVLVGRAMERAVTVARECGYL
                     DGIPEFLSKEMFSQLPRDELVVLATGSQGESRAATARISEGSHPVVTLNPGDKVIFSS
                     RTIPGNEREVGRIINNFIRSGIDVVTDRTALVHASGHPRRGEVAQLYSWTRPHIAIPA
                     HGEDLHLAEHASFAKAQGVQNVIAARNGDIVLLAPGKPGIVGKVPNGRLCKDGNSLIS
                     ASDEALRTRQKLAVSGIVTIALAVTPKGDLAGTPDVVTAGIPARTGGGAAIDTIVDDA
                     LFQTFDQLPRPKRRDADIVSNAIGKAVREAVAAAWGKRPAVQVLVVEV"
     gene            complement(4904..5713)
                     /locus_tag="VNY06_02395"
     CDS             complement(4904..5713)
                     /locus_tag="VNY06_02395"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012591917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HWX13720.1"
                     /translation="MGVALFCLSRSAQDQAFRLVHKSELASTNGDALARAKEGDPGHL
                     WVVADTQTAGRGRNGRSWASPTGNLYASLLLRDPSPQHKAAELGFVAGVAAAHALREI
                     LRGDPRIKIKWPNDILYDGAKLCGILLEGTTLPNGRFACVVGIGANCRTHPKNTPYPA
                     TDLMTIMGLPAAPEVVFEQLSASMAHWLGVWASGADFASIRAEWLPLAAGRGTRIRIA
                     LPSHAVEGAFQTIDEAGRLIVVQDTGKVVTMDAGDVFFAQQSMTAAKAVNG"
     gene            complement(5726..7159)
                     /gene="nuoN"
                     /locus_tag="VNY06_02400"
     CDS             complement(5726..7159)
                     /gene="nuoN"
                     /locus_tag="VNY06_02400"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012385349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HWX13721.1"
                     /translation="MSLLSTALAHCLPELILAVGAVGLVLFGAIRGKDSDSLVTELAS
                     GLLAVAIVVILLGTKTDAVVLNGAFIDDSFGRFMKVFTLAGSLVTLVMSQDFLAKEKI
                     DKFEFPILILLATLGMLMLISAAGLIALYLGLELMSLALYVLAAFHRDNIKATEAGLK
                     YFVLGALSSGILLYGASLLYGFAGTVSFAGILAATASGASLGVTFGLVFIMAGLAFKM
                     AAVPFHMWTPDVYEGAPTPVTAFFASAVKVAAIAITLRVILTAFEGIPGQWRQIVIFI
                     SILSMVLGAFAAIGQANIKRLMAYSAIGHIGFALVGLAAGGEAGVASVLAYMAIYLVM
                     TLGTFAGILAMRVNGKGVENIADLSGLARTDGVMAFFLATMMFSLAGVPPLAGFFAKW
                     YVFNAAIQAGLYPLAVIGILSSAIGAYYYLRVVKVMYFDEPAPAFDRPAPAPRAVLAV
                     TGLMVVFLFAYPSLFIEATRAAANSLF"
     gene            complement(7173..>8482)
                     /locus_tag="VNY06_02405"
     CDS             complement(7173..>8482)
                     /locus_tag="VNY06_02405"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020175213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HWX13722.1"
                     /translation="EKGWFGYGLTYKMGVDGISLPFVLLTTFLMPICILASWQSIQNR
                     VKEYLVAFLVLETLMVGVFCALDLVLFYLFFEGSLIPMFLIIGIWGHARRIYASFKFF
                     LYTFAGSVLMLLAVMAMYGVAGTTDIVALLQTHFPSSMQKWLWLAFFASLAVKTPMWP
                     VHTWLPDAHVEAPTAGSVILAGVLLKMGGYGFIRFSLPMFPEASAYFTPLVFALSVVA
                     IIYTSLVALVQEDMKKLVAYSSVAHMGFVTMGLFSVTAQGVQGAVFQMISHGLVSSAL
                     FLCVGVVDDRMHTRDIAAYGGLVARMPLYAAIFMIFTLANIGLPGTSGFIGEFLTLIG
                     TFKVNSWVVLLAATGTILSACYALYLYRRVIFGVLDKPSLAAISDLSWREAAVFAPLV
                     IFTIFYGVHPAPVLDSSAASIDALIKGYDTSLAVTKAAALTLP"
BASE COUNT         2074 a         2411 c         2345 g         1652 t
ORIGIN      
        1 caccgctgag gccattcaga tgttggtaat tccgaaattg ctgaacaaat cagaatgcgg
       61 ttccggctgg aacattccgg cgtgtgcaat tcctgcatct gcggttgtgc cagtaagcgt
      121 gataatctat ttccgtggca cagaagcttg gtgcgctcgt cgctgtagct gtctctgatg
      181 aggaaggcaa gcgagtgaag ttacaactca aatcgttttc gccatgaggg acctaaaaag
      241 aaaacgcgtt ttagatgcgg cgaaccctat gtcgcaaggc cagggccgcc cgtttccgct
      301 ctgcggcgcg cagccaccaa tggctaatcc ccgcgcgtgg gttaggcttc tttccggcaa
      361 acgcctcgat ctcttaaacc caacgccttt cgactgggag gatgaggacc tcgcattagg
      421 gcttgccagg acttatcgct ggggcggcca ctctgcttgg cccttgccgc tgtcggtggc
      481 acagcattcg ctcctcgtgc ttgccttgca ccgccagcgc cacaaagata ctgcaattgc
      541 cccagccgag cttcgcgaac tgctgcatga tgcggatgaa ggtctcctcg gcttcgattg
      601 tatttccgtg gtaaagccgt ttctcggcga ggcctatcgt ttattgaccg agcgattggc
      661 aaacgcgatc gctctccgtt accgtttgcc gccatggact tctgccgata gaaaggcgca
      721 taggcaagcc gactgggtcg ctgccgcaag tgaagccgtc catgtcgttg gttggacgca
      781 gagcgaggtg cgcaatacct tgcgcatacc ctatcaggcg ctttcaacgg atccattgtg
      841 cacaatctat ggcggcaaag cttgggagcc atgggcaccg cccgttgcca ctgagcggtt
      901 tttggtcgag cttaagcgct tagtcagccc acaaagttag gctttagctt ttggtctagt
      961 cttagctcat cacaatcgcg cgaggtggca ttgaaaagca acgcctgagg attcccgtca
     1021 ggcacaaatt cgtctattgg atgcgcgatc tttagcgagc ttccggaggg ttcgattgat
     1081 gccccgcata catgtatgcc cgctgtccag attgccccac acggcggaag cgtccggtgc
     1141 gcgaagcctc gtcacgctca tcgatgaagg tacgcctgtc gacagaccaa ccggcattgc
     1201 cgccgaaaaa catttggcgc tgattctttc agacatctct gtcgaaacct ttggccatac
     1261 gcttcctaat gactctcatg tcgaaaagct cttggccttt gtgcggcact gggaccaggc
     1321 ggcgcctcta ttgatccatt gcttcgcggg cgtgagccgg tcgacggcgg ccgcttttat
     1381 cgccgcctgc gccttgctcc cacatcgcga cgaggcagag atcgcattgg ccgtgcggaa
     1441 aaattcgcca accgcgacac ccaatccccg gcttgttgcc atcgctgaca agttgcttgg
     1501 acgcaaacag cgaatgactt ccgccgttgc aagaataggc cgcggcgcgg actgttccga
     1561 gggaatccct tttgctctgg agttgcgttg aatctgaagt gcagttacag cagtgatacg
     1621 aaatcgggag tggttcgtgt gaaggaagcc gaaacacaga ggcaagtttg actgcaaccg
     1681 acacagatat cgccgcgaag tcgtttgagg atgcactcaa ggaactcgaa cagatcgtgg
     1741 ataggctcga aaaaggcact gtggggctcg aggaatccat tgcaatttac gcccgcggcg
     1801 aggctttgaa gtcgcattgc aacgcgttgc tccacaatgc cgagcagaga attgaaaaaa
     1861 tttcgctcgg gcccgatggc cgtccagccg gcaccgagcc gctcgatgtg gaataatgcc
     1921 gggatcgctg gtagctaagc ccaattctct cgccaatggg ctgcgtctcg ccccagcaag
     1981 acaaagaatg gattccatgg ctgaatatgc cttccccccg ccgccgttga tcacgcttcc
     2041 agcgccgccc ggaaaacctt ttcccgtccg gcgtattttc tgtgtaggcc agaattatag
     2101 ggaccacgct aaggagatgg gaggcaatcc tgataccgat ccgccgttct tcttcatgaa
     2161 accagcgagc gctattgttc agaaccgcac aacaattccc tacccgccgg ggagccgcga
     2221 tttgcaacat gaggccgagc tggttgtcgc cttgcaagcg ggtgggcgaa acattccggt
     2281 cgaaaaagcc aatgatatga ttttcggcta tgcggtcggc ctcgatatga cgaagcgcga
     2341 cgtccaagcc gaattcaggg aaaagggaag gccttgggag atggccaagg ccttcgactt
     2401 ctccgcgccc tgttctgcga ttgcgttgaa gatccaaacc ggcgctattg ccaagggcaa
     2461 aattgagtgc atggtaaacg gcgagctgcg tcagagcggc gatctctccc atatgatatg
     2521 gggggttcca gaaattgtcg cttatctttc gcagcaagtc gaaatcgcat cgggtgatct
     2581 catctttaca ggcacgcctt cgggcgtagg gcctgttaca aagggcgata ggatcgaggc
     2641 taggatcgcg gggctggagc cgctcgtcat cgagattggc tgagcctgaa ccaattagcg
     2701 ctgctcgagt tcgatgaggg tcccggcgca atctttcgga tccaggaaaa gcaccgcttt
     2761 cccatgcgca ccaattttag gaatgccatc gccaaggaca tgcgtgcctg cgcctatgag
     2821 ctgctcccgg gcggccaaaa tgtcctcaac ctcaaaacaa atatggtgga tggcgccgcc
     2881 gggatgatga gccagaaagc tggtgatcgg ggaatggtca ccgagcggct cgatcagttc
     2941 gattctggta ttcggaagct tgacgaatac caccctcacg cggtggtctg gcaaggcggt
     3001 gggattgctc acctttgctc caagcgcgcc gcgatagaga gcactcgcag cctcaagatc
     3061 cttgactgcg atagcgatgt gattcaagcg tccaatcaca caaattgttc ctttacttga
     3121 tcacacctcg accaccagaa cctgaactgc gggccgcttg ccccatgccg ccgccacagc
     3181 ttcgcgcacc gccttcccga ttgcgttgga gacgatatct gcgtcgcgcc gctttgggcg
     3241 gggcaattgg tcgaaggtct gaaagagagc atcgtcgacg atcgtgtcga ttgcggcacc
     3301 accacccgtt cgtgccggta tcccggcggt tacgacatcc ggcgttccgg cgaggtcgcc
     3361 cttcggggta accgccaagg cgattgttac gattcccgaa accgccagct tctggcgtgt
     3421 tctcaaggct tcgtcactcg cggagatgag actattgccg tccttgcata agcggccgtt
     3481 gggcactttt ccaacaattc caggctttcc cggcgcgagc agcacaatat cgccgttgcg
     3541 ggccgcgatc acattttgga cgccttgcgc tttggcaaag gatgcatgtt ccgcaagatg
     3601 gaggtcctca ccatgcgcgg ggatcgctat atgcggcctt gtccaggaat aaagctgtgc
     3661 gacttcgccg cggcggggat gccctgatgc gtgaacgagc gcggtacgat cggtaacgac
     3721 gtcaatgccg gaacgtatga aattattgat aatacggccg acttcccttt catttccagg
     3781 aatcgtgcgc gaggagaaaa tgactttgtc gcctgggttg agcgtaacca ccggatggct
     3841 gccttcggaa attcgggcgg tcgcggcccg gctttcgccc tggcttccgg ttgcaagaac
     3901 aaccaactca tctcgcggca gctgcgaaaa catctccttc gacaggaact ctggaatgcc
     3961 atcgaggtag ccgcactccc gtgcgacagt gacggctctt tccatagcgc ggccaaccag
     4021 tacgacagcg cgccccgccg ccatggcagc ctcggcgaca gcacgaatac gcgctacatt
     4081 cgaggcaaaa atggtgacaa tgacgcgacc cgacgcagct ccgatgatgt cgcgcaatga
     4141 cttggcgacg tcggcttctg aggggctcaa tccgtcccgc aaaatattcg tcgaatcaga
     4201 aacaagcgcc aaaacgccct ccgcgccgat ggcagcaaga cgctttgcgt ccgtgacttt
     4261 gccaattccc ggggagggat cgattttcca atcgccagaa tgaaggatcg tcccaagcgg
     4321 cgtgcggatg gcaagagcgc aactttctgg gatggaatgc gccataccaa caaattcgat
     4381 gttgaaaggc ccgatcacct ttacctcacc ttgcgcgaca atgctgaatt gaacatcggg
     4441 cgatccgggc tcgccgaccc gtttggcgcc gaaaagtgcg gcggcaaagc tcgtcgcgta
     4501 gagcttgcat ccaagccgag gccagatttc ggtgagagcc ccgatgtggt cctcatgcgc
     4561 atgtgtgatg acgaggccgg ccaaatcgcc ccgcatcttt tctataaagc taatgtctgg
     4621 gatgacgata tcgatgcccg caagatcggg accggcaaat gcgacgccgc aatcgactac
     4681 aagccatttc cagcttccct tgcggccgta gccgtaaagg gcgaggttca tgccgatttc
     4741 gccaaggccc ccaagcggca caaagacgag ctcgtcatat ccatcattca tttccgaaaa
     4801 ccttccaccg gcaggtgccc cgagccggat gcgcctcccg gctaatgttg aaacaacata
     4861 aagagataca caacaatatg aaatgcagcc cgaagtgctt agcctatcca ttgaccgctt
     4921 tggcggcggt catagattgc tgcgcaaaga aaacatcccc cgcgtccatc gtcacgacct
     4981 tgcctgtgtc ctgcacgaca atgagccgtc cggcctcatc aattgtctga aaggcgcctt
     5041 cgaccgcgtg cgaaggcagt gcaatacgga tccgagtgcc acgcccggcg gctaaaggca
     5101 gccattcggc gcgaatcgat gcaaagtccg cgcctgaagc ccagacgccg agccaatgtg
     5161 ccattgacgc cgaaagctgc tcaaacacaa cttcaggtgc agcgggaagc cccataattg
     5221 tcatgagatc ggttgccggg taaggcgtat ttttgggatg cgtccggcag tttgcgccta
     5281 tacctacgac gcaagcgaac cgtccattag gaagagtcgt accttccagc aagattccac
     5341 aaagtttggc accgtcgtag agaatatcat ttggccattt gatcttaatt cgcgggtctc
     5401 cccgcaagat ttcgcgcaaa gcatgtgcgg ccgcaacgcc agcgacaaat ccgagttcgg
     5461 cggccttatg ttgcggcgac ggatcacgca aaagcaggct cgcataaaga ttgccggttg
     5521 gagatgccca cgaccggcca ttgcggcccc ggcccgcagt ttgggtgtcg gcgacaaccc
     5581 acagatgccc cggatcaccc tctttcgcgc gcgccaacgc atcgccattg gttgaggcga
     5641 gttcgctttt gtgaaccagg cggaatgctt gatcttgggc ggatcgggaa agacaaaaca
     5701 gggcaacacc cactatgcgc aaaattcaaa acagcgagtt cgcggccgct ctggtcgcct
     5761 cgataaagag gctcggatag gcaaacaaaa acacaaccat aagtccggtc accgcaagaa
     5821 ctgcacgcgg agccggagct ggcctgtcga aagcaggcgc cggctcgtcg aaatacataa
     5881 ctttgacgac ccgaagataa tagtaagcac cgatagcact cgacagaatg ccgatgacgg
     5941 caagcggata aagccctgcc tgaatggctg cattgaaaac ataccatttg gcaaaaaagc
     6001 cggcgagcgg cggtacaccg gccaaggaaa acatcatcgt tgcaagaaag aacgccatca
     6061 ccccatcggt acgggcgaga ccggaaagat cagcgatgtt ttcgacgcct ttgccattaa
     6121 cccgcatcgc tagaatgcca gcaaaagttc cgagcgtcat gacaagatag atcgccatat
     6181 aggcaaggac gctagccact cccgcttcgc cgcccgcagc aaggccgacc agcgcgaagc
     6241 cgatatggcc gatcgcagaa taagccatca gccgcttaat attggcctgg ccaattgcgg
     6301 caaaggcgcc aagcaccatc gaaagaatgg aaatgaatat gacgatctgc cgccactgcc
     6361 cgggtatgcc ctcgaaagcc gtgaggatta ccctcagcgt tatggcgatc gcggccacct
     6421 tcaccgctga agcgaaaaac gcggtcaccg gcgtcggcgc gccttcatat acgtctgggg
     6481 tccacatgtg aaatggcacg gccgccatct tgaaggcgag tccggccatg ataaacacaa
     6541 ggccaaaggt aacgccgaga gaggcgccgc tggcggttgc cgccagaatg ccggcaaagg
     6601 aaacggtgcc ggcaaatccg tacagcaagg aagcgccata aagcagtatg ccggacgaaa
     6661 gcgcgccaag gacaaaatat ttgagtccag cttccgtggc cttgatgtta tcgcggtgga
     6721 aagccgctag gacatagagg gcgagcgaca tcaattcaag cccaagataa agggcgatca
     6781 atccagccgc cgaaatcagc atcagcattc cgagcgtcgc caaaagaatg agaatcggaa
     6841 actcaaattt gtcgatcttc tccttagcaa ggaaatcctg gctcataacc aaagtgacaa
     6901 gcgacccggc aagcgtaaac accttcataa accgcccgaa gctatcatct atgaaggcac
     6961 cattaagtac aaccgcatcg gtcttggttc cgagcaagat gaccacaatg gcaactgcaa
     7021 gcagaccgga agcgagctcc gttacaagac tatccgagtc cttgccacga atggctccaa
     7081 acagaacgag cccgactgcg ccaaccgcca gaatgagttc gggcaagcaa tgcgcgaggg
     7141 ctgtggaaag caatgacatc tgatctcagc ctctagggta atgtaagcgc tgcagcctta
     7201 gtcacagcga gagaggtgtc gtaaccctta atcagagcat cgatcgaggc cgccgaacta
     7261 tcgaggacag gagccggatg gacgccatag aagatggtaa aaataacgag cggcgcaaac
     7321 acggctgcct cgcgccatga gagatcgctt atcgccgcaa ggcttggttt gtcgagcaca
     7381 ccaaaaatga cccggcgata aagataaagt gcgtagcagg ccgataaaat ggtaccggtg
     7441 gccgcgagca gcacgaccca actgttgacc ttaaacgtcc cgatgagcgt caggaattcg
     7501 ccgatgaatc ccgaggttcc gggcaggccg atattcgcga gcgtaaagat catgaaaatg
     7561 gctgcataaa gcggcatgcg tgcgacgagg ccaccatagg ccgcgatgtc tctcgtatgc
     7621 atccggtcgt cgacgacgcc gacgcagaga aatagggcgc ttgagaccag accatgcgag
     7681 atcatctgaa acaccgcgcc ctgcacgccc tgcgcggtga cgctaaaaag acccatcgtc
     7741 acgaagccca tatgagctac cgaagaatag gcgacgagct ttttcatatc ctcctgcacc
     7801 aaggcaacaa gcgaagtgta gatgatcgcg acaaccgaca atgcaaagac cagcggcgtg
     7861 aaataggcag aggcttccgg gaacattgga agcgagaagc ggataaaacc ataaccaccc
     7921 atcttgagca ggacacccgc caggataacc gaacccgcgg tcggcgcttc gacatgcgcg
     7981 tcaggaagcc aagtatgaac tggccacatc ggtgtcttga cagcgagtga ggcaaagaac
     8041 gcgagccata accatttttg catcgacgat ggaaaatgcg tctgtagcaa ggccacgatg
     8101 tcggttgttc ctgccacgcc atacatcgcc atgaccgcaa gcagcatgag caccgagccc
     8161 gcgaacgtat aaagaaaaaa cttgaagctc gcatagattc gccgcgcatg cccccaaatg
     8221 cctatgatga ggaacatcgg gataaggcta ccctcgaaga aaagataaaa gagcacgaga
     8281 tcgagagcac aaaagactcc aaccatcagt gtctcgagaa cgaggaaagc gaccaaatat
     8341 tccttgacgc gattctgaat cgattgccac gacgcgagga tgcaaattgg catcaaaaaa
     8401 gtggtcagaa ggacgaaggg aagcgaaatt ccgtcaacac ccattttata ggtgaggcca
     8461 tagccaaacc agcctttctc ct
//
LOCUS       DATMPU010000105         4074 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_384465,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000105 DATMPU010000000
VERSION     DATMPU010000105.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 4074)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4074)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4074
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_384465"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..796)
                     /locus_tag="VNY06_02410"
     CDS             complement(<1..796)
                     /locus_tag="VNY06_02410"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HWX13723.1"
                     /translation="MHNAVHIIGAGLAGLAAAVRLAGGPRNVVLHEAAWQAGGRCRSY
                     FDSSLGMTIDNGNHLLLSGNHDALAFLAAIGGADQLSWPCEANFAFIDYRTKERWHLH
                     PNAGPFPWWIFSPHRRVPATGARDYLELLRLLLSSNDMPLDTVLRRRGMAYQRLWRPF
                     FLAALNTDLAEGSSHLAAGIVWETLARGGRACRPLVAARGLSAAFVDPALAYLVNRGV
                     MVSFEHRLRAIALANGRVAALDFGEDSVSLEAADKVILAVPSYAARE"
     gene            complement(796..1647)
                     /gene="hpnD"
                     /locus_tag="VNY06_02415"
     CDS             complement(796..1647)
                     /gene="hpnD"
                     /locus_tag="VNY06_02415"
                     /EC_number="2.5.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020177155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016767 - geranylgeranyl-diphosphate
                     geranylgeranyltransferase activity [Evidence IEA];
                     GO_process: GO:0016117 - carotenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="presqualene diphosphate synthase HpnD"
                     /protein_id="HWX13724.1"
                     /translation="MSGASGVAQRAGQRARGSSFNAAMRILPRPRREAMFEIYSFCRA
                     VDDIADEAGPREARRQALAQWRRDIDALYCRSGIRHSTREGLEGLNQAIRAFSLRRED
                     FCAVIDGMEMDVETDIRAPDFATLDLYCDRVASAVGRLSVRAFGILQEEGQDLAHHLG
                     RALQLTNILRDLDEDAEMGRLYLPREDLRAARIEENEPRKVLANPALDLACASTVARA
                     KAHFAKAQEIMARCSRECVRAPRIMADVYSAILTRLEKRGFAPPRPRIRAPRHRILLA
                     ILRYGLL"
     gene            complement(1644..2522)
                     /gene="hpnC"
                     /locus_tag="VNY06_02420"
     CDS             complement(1644..2522)
                     /gene="hpnC"
                     /locus_tag="VNY06_02420"
                     /EC_number="2.5.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011315493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004311 - farnesyltranstransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="squalene synthase HpnC"
                     /protein_id="HWX13725.1"
                     /translation="MKADLLVKPTKTHKGENFPVASLLIAPKHRAAILAYYRFARAAD
                     DVADSPSLTPDDKLAGLDLFEATLLGHCDTIKSALPLRVVLAERKLPPRHALDLLRAF
                     RMDAVKQRYADWSELMNYCAYSAAPVGRFVLDVHGESEVTWPASDALCTALQIINHLQ
                     DCGADYRKLNRIYIPQDVLAAQGLGAEALGAAKASPALRMALHETAQRNAPLVAAGAK
                     LPQAVRDFRLCLETAVIAQLARRLNSFLLERDPLSEDVHLTRLRSLIYSGFGVAQGLR
                     AFLRLRRVAATQAGGA"
     gene            complement(2519..3511)
                     /gene="hpnA"
                     /locus_tag="VNY06_02425"
                     /pseudo
     CDS             complement(2519..3511)
                     /gene="hpnA"
                     /locus_tag="VNY06_02425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012592215.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hopanoid-associated sugar epimerase"
     gene            3708..>4074
                     /locus_tag="VNY06_02430"
     CDS             3708..>4074
                     /locus_tag="VNY06_02430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012592213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HWX13726.1"
                     /translation="MNKTSSPGTKSASPDVKSSPRALIRLLPYLLRYKPLIVAALIAV
                     ATAAAATLAVPLAIRRMIDHGFSAASASLIDTYFAGLIAVAGILALASGSRYYFVTIL
                     GERIVADLRTDLFRHLCRLD"
BASE COUNT          865 a         1199 c         1216 g          794 t
ORIGIN      
        1 tctcgcgcgc ggcataggaa ggaacagcaa gaatgacttt gtctgctgcc tcgagtgaca
       61 cgctatcctc gccgaaatca agagcagcga cacggccatt ggcaagggcg attgcgcgaa
      121 gcctatgctc aaagctgacc attacgcccc ggttgacaag ataagccagc gccgggtcga
      181 cgaaggcggc agagaggccg cgcgcggcga cgagaggccg gcaggcgcgc ccgcctctgg
      241 caagtgtctc ccacacgata ccggcggcaa gatgagacga gccttcggca aggtcggtat
      301 ttaaggccgc aagaaaaaat ggtcgccata accgttgata tgccatgccg cggcggcgca
      361 ggaccgtatc gagcggcata tcattgctgg atagcaaaag gcgcaagagt tcaagataat
      421 cgcgcgcacc ggtggccggc acccgccggt ggggtgaaaa aatccaccaa ggaaatggcc
      481 ccgcatttgg gtgcagatgc cagcgctctt tggtcctgta atcgatgaaa gcaaaattag
      541 cttcacaggg ccaagataat tgatccgcac cgccgatcgc agcgaggaag gcaagcgcat
      601 cgtgattgcc cgacaagagc aaatgattgc cattatcgat tgtcatgccg agcgaggaat
      661 cgaaataaga acggcagcgc ccgcccgcct gccacgcggc ctcgtgcaga acgacatttc
      721 gcgggccgcc agcaaggcgc accgctgcag caagcccggc aagccccgcg ccaataatat
      781 gcacggcatt gtgcatcaaa ggagtccgta acgcaggatc gctagaagaa tgcggtggcg
      841 gggtgcgcga atgcgcgggc gaggcggcgc aaacccgcgc ttttctaagc gcgtcagaat
      901 ggcggagtag acatctgcca tgattctcgg cgcgcgtacg cattcgcgcg agcagcgcgc
      961 catgatctct tgcgcctttg cgaaatgggc ctttgcgcgg gcaacggttg atgcacaggc
     1021 gaggtccagc gccggattgg caaggacttt tcgcggttca ttttcttcta ttcgcgccgc
     1081 gcggagatcc tcccgcggca ggtagagcct tcccatttcc gcgtcttcgt cgagatcgcg
     1141 caaaatattg gtgagctgga gtgcgcggcc aagatgatgt gcgaggtctt gaccttcctc
     1201 ctgcagtatc ccgaaggcgc gcacggaaag tcgcccgacg gcgcttgcca ctcggtcgca
     1261 ataaagatcg agggttgcaa aatctggcgc gcgaatgtcg gtttcgacat ccatttccat
     1321 gccgtcaatc acggcgcaaa aatcctcccg ccgcagacta aaggcgcgga tggcttggtt
     1381 caggccttct aatccttctc gcgttgagtg cctgatgccg gaacggcaat agagcgcatc
     1441 gatgtcgcgg cgccattgag caagcgcttg cctgcgcgcc tcccgtggcc ccgcttcatc
     1501 cgcgatgtcg tcgacggcgc ggcagaagga atagatttca aacatggcct cgcggcgggg
     1561 ccgaggaaga atgcgcatcg cggcattaaa ggagctgccg cgcgcgcgtt gcccggcgcg
     1621 ctgagccacc ccggatgcgc cgctcatgca ccaccggcct gcgtcgcagc aacccgccgc
     1681 aaacgcaaaa aagcccgcag cccctgggca acgccgaagc ccgaatagat aagcgagcga
     1741 agtcgcgtca aatgaacgtc ttcgctcaag ggatcgcgct cgagcaggaa ggaattgagc
     1801 cttcgagcaa gctgcgcgat cacggcggtc tcgaggcaca aacggaaatc ccgaaccgct
     1861 tgcggcaact ttgcgccggc cgcaacgagc ggtgcgtttc gttgcgccgt ctcatgcaga
     1921 gccatgcgca gagccggaga ggcttttgcc gctccgagcg cctccgcgcc aaggccttgc
     1981 gcggcgagca cgtcttgcgg aatgtaaatg cgattaagct tgcggtaatc ggcgccacag
     2041 tcttgaagat ggttgatgat ctgaagcgcc gtgcaaagcg catccgacgc gggccaagtc
     2101 acttcgctct cgccatgcac atcgagaacg aatcgcccaa ccggcgcagc cgaataggcg
     2161 caatagttca tgagttcaga ccaatccgcg tagcgctgct tgacagcatc catccgaaag
     2221 gcgcgcagca agtcgagtgc atgacgtggc ggaagctttc gctccgccag aacaacgcgg
     2281 agcggcaatg cggatttgat cgtatcacag tgtcctaata atgtcgcttc aaacagatcg
     2341 agtcccgcaa gcttgtcatc aggcgtaagt gagggactgt cggcgacatc atcggcggcg
     2401 cgggcgaagc gataataagc gaggattgcg gcacggtgtt ttggcgcgat gagcaaggag
     2461 gcgacgggaa aattttcccc cttgtgcgtt ttcgtcggtt taactagcag gtcagctttc
     2521 atcgcaatcg tccattggca cggaaccacg ccagtgcgtc tttgagcgct tcggtgtagg
     2581 gccgcgcctt gtaaccgagg tcgcgttcgg ccttggcaga gctaaagaac atgcgatgct
     2641 ttgacatccg caatccatcg actgtcaaga aaggttctcg acttgtgtgc catggcttgc
     2701 gccgccaaag caaagggata cattagatga cgcggcaggc gtaattttgg cggctcacgg
     2761 ccgcacatcg ccgcaatctc ggctagaaaa tccgcgaagg tgacgttttg tccgccgaga
     2821 atatagtggt ctccgggctt ggcgtgcagc agggcggcga ggtggccgtt tgcgacatcg
     2881 tcgacatgca cgacattaag acccgtgtcg acaaacccgg ggatgcggcc ggacgccgcc
     2941 tctatgacga tccggccggt tggtgttggc ctctgatccc gcggtccaat cggtgccgtc
     3001 ggatgcacga taatggctgg caaatcttgc gaggtgatca gttcctcgac gattcgctcg
     3061 gcagcgatct tgctttgctt ataggctccg atcgcgtatc gttcggtctg ctgcatcgtt
     3121 tcgtcagcgg gcggtccgcc ggccgaacat gcaatggttg ccacgctgct cgtgtaaacg
     3181 atgcgctcga ccccttgcgc cagggccgtt tgcatcaaaa ccctggtccc ttctttgttg
     3241 ttgtgcagaa tttcttgcgg tttgcgcgcc caaaggcggt agtcggcggc aacatggaac
     3301 agaaaacgca cgcctgcatg gcccgcgcga cgaccaaagg atcacgcatg tcgccttctg
     3361 caatctctaa atcaagacca ccaagatttt tccggctgct cgcttggcgc agcaaagcgc
     3421 ggacgcgata gccagagcgg acaagggcat gcgctacggc ggacccgaca aagcctgaag
     3481 cgcccgtgac cagaaccttg tccgctgcca ctccgcttga cctccattac aacggatgca
     3541 atccccatac ctatatcggg ccaagtttac gcccctatcg cgtcaatcga agtgtctgct
     3601 ttgcctgatc agtattggtg cccgctggca ttgggtggac gctttcgagg cgtcggtgta
     3661 gggatgaatt tcaactggcc gacaaaattc aatagtataa ccgcgcaatg aacaaaactt
     3721 cctcgcctgg gacaaaatcc gccagtccag atgtcaaatc atccccccgc gcgctcatcc
     3781 ggcttttgcc ctatctcttg cgctacaagc cgcttatcgt cgcggcgctg attgcggttg
     3841 cgacggccgc ggcggcgact ttggcagtgc cactcgcaat ccggcggatg atcgaccacg
     3901 gcttttcggc cgcgagcgcg agcctgattg acacctattt tgctggcctt attgccgtgg
     3961 ccggtattct ggcgctagct tcaggatcgc gatactactt tgtgacaatt ctcggggaac
     4021 gtatcgtcgc cgatctccgc acggatcttt ttaggcatct ttgccggctc gacg
//
LOCUS       DATMPU010000106         4590 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_2250497,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000106 DATMPU010000000
VERSION     DATMPU010000106.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 4590)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4590)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4590
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2250497"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(558..809)
                     /locus_tag="VNY06_02435"
                     /pseudo
     CDS             complement(558..809)
                     /locus_tag="VNY06_02435"
                     /inference="COORDINATES: protein motif:HMM:NF014430.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            1396..1782
                     /locus_tag="VNY06_02440"
     CDS             1396..1782
                     /locus_tag="VNY06_02440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX13727.1"
                     /translation="MTNDDVHEDRARVIVDAFLQNKSMEQKQDYLARGRRFGKLDVAQ
                     LNEGWIMAVGSWLAHKSQRDELLMDDLASELRLRRLEPPYRAVEKELADRFIQADGSK
                     REEKIRNVVQEIYDFMNKNERYSTSS"
     gene            1950..2930
                     /locus_tag="VNY06_02445"
     CDS             1950..2930
                     /locus_tag="VNY06_02445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009490784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HWX13728.1"
                     /translation="MAGAVAVALAAAPVLTCGPACAQVKWNLLSAYPADNFHSQNLEA
                     FAKDLAEATGGRLLIRVYPNASLHPASAIKTAVRIGQAQMGEILISLHDNEEPIFGID
                     AVPFLATSYHEARNLWAVSKPLIERKLAGQGLMVLFAVPWPPQGIFAGKAIDQIADMK
                     GLSWRVYNAATQRIAQIVDAYPVTIQAADLEQALATGLISALMTSAATGYDVKVWDRL
                     GYFYDTRAWLPKNVTLINKAAFDGLDKPTQESVLKVAAAAEARGWWWSQEKTKWYTEQ
                     LAAHGMRVLSPSMALRTGLEQIGERVTQEWLMRAGAEGLATIDAYRRRTM"
     gene            complement(3256..3774)
                     /locus_tag="VNY06_02450"
     CDS             complement(3256..3774)
                     /locus_tag="VNY06_02450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX13729.1"
                     /translation="MLYPYPPRCSALYQKLRNQPDTVKGAITDRSNTQWQCDLSINRT
                     DVQPVGFSRDMGIGKMFSAMQYDRLIERTLRDAQGVLRANLPPAKNLSDNQAVQSLRA
                     IVRAPQVREVLERGQDTALCFVLRAVNRILADDGQPDRMTINRLWDVLDEPEFNRMLG
                     RLQNMKKPPAQP"
     gene            3947..4129
                     /locus_tag="VNY06_02455"
     CDS             3947..4129
                     /locus_tag="VNY06_02455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX13730.1"
                     /translation="MATKSDQKTLQRKSDELDEIIAMFAETHAQTVELRRMLERTCRR
                     AEAALALAREIRRLRL"
     gene            complement(4382..>4590)
                     /locus_tag="VNY06_02460"
     CDS             complement(4382..>4590)
                     /locus_tag="VNY06_02460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX13731.1"
                     /translation="HYLRLSSLPNPPGHLLVHSSPKAVIAITKRRFETPDKRGCPKSV
                     NLDIYAANIAWMALATPKRVKPTP"
BASE COUNT          976 a         1284 c         1325 g         1005 t
ORIGIN      
        1 ctgaacgttg cacctcgaca attactgccc agcttaattg cctggcgaca ctctttccac
       61 tttcaatgcg gggtctcgca tgcgcgcgag catcaatttt gatctgcaaa tgcatcaacc
      121 gggcaatgac gtggacgacc ggcggctttt ggaattatgc gccgagtggc aaatggcgcg
      181 ggcacagcaa gagataaatt gggccgagca gaatttggcc agcaggcaag tgtgcagtgc
      241 aaactcctga gctgcccatg tctcagatgg gacatgtatg gacggcgccc gcggtgcaag
      301 aaaaaagaat ctgacctctc agcaaaacgt tcgggtgcag ccatgtatcc ggcctttgaa
      361 tgcagccgtt gtggctgctc gcccggatgt gatccgctga tcggtcccaa acaaaagcac
      421 gcgtttcttt cctcgcgtgg cactcagcgg gttttcccga tcgacggtct cgaccgtttc
      481 gcatcaacgt catcatcacc ccttgcagtt cgtgaacgca ccgacgcaac ctcatcctcc
      541 cgttgccaaa tcctttctta cgccgccgca agccgtggcg cccggtagcg ccctccactg
      601 gccaaaaacg cccaggcgat gcgggccatc ttgttggcaa gcgcaatggc cacgaccttg
      661 aacggtcgcc gtgccaggag ctgcgtcagc cacggatatt tctccgggtt ctgcctggcc
      721 cgccgaagca cagaatgcgc cgcgaccaca agaatatgcc gcaggtagcg gtcgccctgc
      781 ttcgagatcg gaccaagctt ctgcttgccc ccggttgaat cttgtcagag gtgtcccgca
      841 gattagaccc cccaagtaat tgaaaagcta agcttttcac caaaacagcg gaaagagctt
      901 ttttgtgatg gctatcacag tttttgtgtg aagggcctca tacagtccca acatgcaaat
      961 tcgggttgcg ggagaatcgc tgtgacaatg acctcatatt tccgttccca ggaagcgcct
     1021 actgggcccc catgcaatca ttccatgtgg cgtaagctct tcgcggctat gactgaggga
     1081 ccaacgccga gggcggaaga cgcaatcgcc gcctttcttc ataagcacca gcacgattta
     1141 gcacccgcgt tgtggatcga actcgagagg cgccgcctag ccccgtaaat cggcagtcct
     1201 cgttcgcacg cggctccggg cctccagatc cgcttgcggt cgatcatgtc gagagtaccg
     1261 ccggctaatt ttgctcggca cgcgacgcat ctgactcgca tggggcgaag tcacggcaat
     1321 tgaacctggc ggcatgtagc cgtgcgcttg agttggccaa gcgatcgatc gttggacaca
     1381 taaaggctag ccgagatgac caacgatgat gtgcacgaag atcgcgccag ggtgattgtc
     1441 gatgcatttc ttcagaacaa gagcatggag caaaagcagg attacctcgc ccgcggacgg
     1501 cgcttcggca agctcgatgt cgcacagctc aatgaaggct ggatcatggc agtcgggagc
     1561 tggcttgctc ataaatctca gagagatgag ctactgatgg atgatctggc gtccgagctg
     1621 cgactgcgca gactcgaacc gccgtatcgc gctgttgaga aagagctcgc cgaccgcttt
     1681 attcaggcgg acgggtctaa gagagaagaa aaaataagaa atgtcgtcca ggaaatttac
     1741 gacttcatga acaagaacga acgctactct acgagttcct agaacgctac tatcgtccaa
     1801 actggtgatc aaatgaatcg catgcggtat gtggtttgca tcggagcgtc gcgggtcacg
     1861 cccgcggagc gctttcttgc agacgctagt ggcgacgtgg ccaaacaccg gaacatcttg
     1921 gtatcatgac cacagtgcga tgctctagat tggcgggggc tgttgcggtc gcgctagcgg
     1981 ctgcgccggt gttgacctgt gggcctgctt gcgcgcaggt caaatggaat cttctctccg
     2041 cttatcccgc ggacaatttt cacagtcaga acctcgaagc tttcgccaag gatttggcgg
     2101 aggcgactgg aggtaggctt ttgatcaggg tctatcccaa tgcttcgttg catcccgcgt
     2161 cggctatcaa gaccgcggtg cggatcgggc aggcgcaaat gggcgaaatc ctgatttcgc
     2221 tgcacgacaa tgaagagccg atatttggaa tcgatgccgt accgttcctg gcgaccagct
     2281 atcacgaggc gcgcaacttg tgggcggtat cgaagccgct gatcgagcgc aagctcgcgg
     2341 gacaaggttt gatggttctg tttgccgtgc cgtggccacc acagggcatc ttcgccggca
     2401 aggcgatcga tcagatcgcc gatatgaagg gcctatcctg gcgtgtgtat aatgctgcca
     2461 cgcagcgcat cgcgcaaatt gtcgatgcct atccggtcac gatccaggcc gccgatctag
     2521 agcaggcact cgccactggc ttgatcagtg cccttatgac ctcggccgcg accggctacg
     2581 atgtcaaggt gtgggacagg ctgggctatt tctatgacac ccgagcctgg cttccgaaga
     2641 atgtcacctt aatcaataag gcggcgtttg acgggctcga caaaccgaca caggagtcgg
     2701 tactcaaggt cgctgctgcc gccgaggcgc gcggctggtg gtggtcgcag gagaagacca
     2761 agtggtacac ggagcagctt gccgcccatg gcatgagagt attatcgccc agtatggcct
     2821 tgaggacagg gctggagcag atcggcgagc gcgtcacaca ggaatggcta atgagggccg
     2881 gcgctgaggg gctggcgaca atcgacgcct atcgcaggcg aacaatgtga tgctttatga
     2941 acgaaaaaga tgcgtcgccg ttcgcggctg cgcattcggc agtcgggccg tacttccttc
     3001 tcaactctac gaaatcccga tcctgattaa cattcccatt gtaaacagcg gctcgtgcca
     3061 cgaccggcgc tcgcaacgcg aggtcacccg ccattgctga gccggagatt gctgttgctg
     3121 cgagaattat caaaatcttg cgcattgcat gattccccac aaatgcattc aaaactcttg
     3181 gggctgatcc gacgcatcgt caggttgacc ttgctctgca gcacgtgcgc tgagatgtgt
     3241 gatacagtac ctcgtttatg gctgagcggg tggttttttc atgttctgta gacgacccag
     3301 catgcgatta aattcaggct catcaagaac gtcccatagc cgattgattg tcatgcgatc
     3361 cggctgtccg tcgtcggcta ggatgcgatt tacggcacgc agcacaaagc aaagtgcggt
     3421 gtcttggccc cgctccaaca cttctcggac ttgtggcgca cgaacaatgg cacggaggga
     3481 ttggactgct tgattatcgg ataggttttt cgctgggggc aaattcgccc gcaggactcc
     3541 ctgagcgtcc cgcaaagttc gttcgatcag gcgatcgtac tgcatggcgg aaaacatttt
     3601 accgattccc atgtctcggg aaaagccaac gggttgcacg tctgtacggt tgatcgacag
     3661 atcgcactgc cattgcgtgt tactgcgatc ggttattgcc cctttgactg tgtctggctg
     3721 atttcgcaac ttctggtaga gcgcactgca tcgcggtggg tacggataca gcatcgccct
     3781 agcccctccg agtgtgagat tggtcgccgt ggtccttggc gggccgcagg gtctagcgtg
     3841 cccgaagata cccggtggag aacaggccag agccggttgc agtcgagatc atcgcatgaa
     3901 tgatgtcgtc cactgaatga tgtcgtcgcg acttgcgagt attgcggtgg ccacgaaatc
     3961 cgatcaaaag acattgcagc gcaaatcgga cgagctggac gagattattg ccatgttcgc
     4021 tgaaacacac gcccagacgg tcgagctacg gcggatgctg gagcgtacct gccgccgcgc
     4081 tgaagcagcc ctcgcccttg ctcgcgagat caggcgcttg cgtctctagg ccgctgtgtc
     4141 ggcggccatt gagggatcgt ttcggagctc atcgacgatc acactcaact cgtccgccaa
     4201 ttgctgtccg ggcaagcgat ctttgaacac cttgccgccc tcgatcggcg catcgatgcg
     4261 gatatacgct tcaatatcat tctcccgtag tgccgtgctc ctcttgcgcc gctcgcactt
     4321 tgctcggcgt tcacgctatc cctgctgctt catccacggg cattaccccg cttccgcgct
     4381 actacggggt tggcttgacc cgtttcggcg tggcgagtgc catccaggct atgttagcgg
     4441 cataaatgtc gaggttaacc gacttgggac agccgcgttt gtctggggtc tcaaaccgac
     4501 gttttgtgat agcgatcaca gcttttggcg aggaatggac cagtagatgc ccaggaggat
     4561 tgggcaatga agataatcgc aggtagtgtc
//
LOCUS       DATMPU010000107         4172 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_385468,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000107 DATMPU010000000
VERSION     DATMPU010000107.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 4172)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4172)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4172
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_385468"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..253
                     /locus_tag="VNY06_02465"
     CDS             <1..253
                     /locus_tag="VNY06_02465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020176130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase"
                     /protein_id="HWX13732.1"
                     /translation="VPRVMPLACDRKLLSQLTQAAFGQRRKMLRQSLKSLSGGNAEAL
                     LAEANINPELRSEEIQIEEFIALAGALKNFRREPGSLPD"
     gene            617..1276
                     /locus_tag="VNY06_02470"
     CDS             617..1276
                     /locus_tag="VNY06_02470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX13733.1"
                     /translation="MARARAFRPRSIGIIMDDFGLAQWVGGPGTYPYKSLVLGNVTYN
                     NGGRGIQVGPTSGNVSVYNNTSYNNNLDTIWAETCRGEVFVWGNSTNVVNNVLYAVPM
                     NTGKITDHNSGICWAGIPDKNDTFSHNLTFNGTVGQNSVTVQGTTNDTVNLNAIIANN
                     TLLGTDPKLANVGTKDFYPLAGSPVLGGGTPGPLGYPAYTVDGFEQPSPPNIGALAVR
                     P"
     gene            1657..2142
                     /locus_tag="VNY06_02475"
     CDS             1657..2142
                     /locus_tag="VNY06_02475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011475185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion associated locus B family protein"
                     /protein_id="HWX13734.1"
                     /translation="MWLALATVSFLASGHSAFCQGVVKSKHGDWDLRCETPPGAAKEQ
                     CALLQSVAAEDKPNINLTVIVLKTADGKNRLLRVIAPLGLLLPSGLGLRIDDIDVGRA
                     GFVRCLPTGCIAEVVMEDKLIEQMKNGKKATFIIFQTPEEGIGIPLSMTGFKEGFDDL
                     P"
     gene            2129..2827
                     /locus_tag="VNY06_02480"
     CDS             2129..2827
                     /locus_tag="VNY06_02480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX13735.1"
                     /translation="MTCRKPGRKSARKRSLARGINPFLAALVLLGLAGCGGHLANFDA
                     PQSPDSNGPAGAKLLATMLGMKKNEAASDVAAAPGPQDRHIFCPEVVLLEGTEASRAY
                     AGTPASSANLRYQFALTETARECALDAGELKLKIGVAGKVLLGPAGSAGSFTVPVRMA
                     ILRKSDNEPEVSKLYHAKVNVGQSETEADFTIVSEPIHVPFIQDHSEEDYSIRVGIDE
                     GPVKDEKPRKGAKP"
     gene            2988..3566
                     /locus_tag="VNY06_02485"
     CDS             2988..3566
                     /locus_tag="VNY06_02485"
                     /inference="COORDINATES: protein motif:HMM:NF023113.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3280 domain-containing protein"
                     /protein_id="HWX13736.1"
                     /translation="MTILYRDGLFATLALLEFSLLKRKVVRTTMLAVMLLFVGSGVAA
                     AQRGSGEKLKVAFFGFELVNTSLQPTTDAEKQRLIVVGDTLTQMLAGSGRYEVVLLSD
                     ALKQKIERSARVTGCNGCQIEWAREAGADLAVWGTVQKVSNLILNENVYIDDARSGRE
                     FFSQSVDIRGNTDESWVRGMRYLLRYKLLEKR"
     gene            complement(3799..4068)
                     /locus_tag="VNY06_02490"
     CDS             complement(3799..4068)
                     /locus_tag="VNY06_02490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX13737.1"
                     /translation="MHYIIVAALSFKHGAFAHESEWRLFALVPRDNTTHVRFDEKKLP
                     YMLPYILMPYNANALAKDLLKEIWLAPAHPNEVDIATTFFVPEVW"
BASE COUNT          983 a         1103 c         1152 g          934 t
ORIGIN      
        1 cgttcctcgc gtgatgccgc tagcctgcga taggaagtta ttgtcgcagt tgacgcaagc
       61 agcattcgga caaaggcgga agatgctgcg gcaatcgctg aaatcgctca gcgggggcaa
      121 tgccgaggcc ttgctcgccg aagcgaacat caatccggaa ttgcggtccg aagaaatcca
      181 aatcgaggaa tttatcgcct tggccggcgc gcttaaaaac ttccgccgcg aaccaggctc
      241 acttccagac taatcgccga aggatccatt tgatcagccg gtctgatcga attgcgtgct
      301 tcgccgcttc gccgagagcg cgggaatatg ccggatgtaa gtgtcgaaga agaggagaca
      361 agcacgaatt gacctcacgc gagatgcatt cgaagtctgt cagtgcgtag cggcgctggc
      421 tcatgatcgc ttctccaagc cgcttgaatc acctttcgcc aaataacaaa atccactcta
      481 tgaagttgtt tcctagagtt tgatggcaac ggcatcggtg tctatgagcc ggtgaagatt
      541 ccaggattta gctccgattt gccatgggac aaccagacat atcatattca ggtccttcaa
      601 aacgtggtgc atgacaatgg cgagggcccg agcattccgg cctcgaagca tcggcattat
      661 catggatgat ttcgggttgg cgcaatgggt tgggggcccc ggcacttatc cttacaaaag
      721 ccttgtactt gggaatgtta cttataacaa cgggggacgc ggtattcagg ttggacctac
      781 ctctggcaat gtcagtgtct ataacaacac ctcatacaac aacaatttgg atacaatatg
      841 ggcggagact tgtcgaggcg aggtgttcgt ttggggtaac agcaccaatg tggtcaataa
      901 tgttctctat gccgttccta tgaatacggg gaaaattact gaccacaact ccgggatttg
      961 ctgggctgga attcccgaca aaaatgacac cttcagtcac aatctgacat tcaacgggac
     1021 cgttgggcaa aatagcgtta ccgttcaggg aacgacgaac gacaccgtga atctgaatgc
     1081 catcatcgcc aacaacacat tgctcggcac tgaccccaaa cttgccaatg ttggcacaaa
     1141 agacttttat cctctcgccg ggtcgccggt gctcggcggc gggactccag gcccattagg
     1201 ctatcctgct tacacagtcg atggttttga acagccaagc ccgcctaata taggcgcgct
     1261 ggcggtccga ccatgaaatc ttcgcggatt ggccgccaaa tgcgaagttg cgaacaacga
     1321 gtccacggag ccacgcgcct gggccttgta ggttttatat ttatgctccc gctaattcat
     1381 agaaaacctc aaatttttct cggccgtggc ggcctctcgt cgcgccggct gtgcccctcg
     1441 cagccgcgcc aaacccctgc ctcgcatcgg ccagcacatt ggattcgcat gccgggccat
     1501 gatcacgaac ttgacggcct cggacaaatt cacccatgag tattatgatt cgggtttctg
     1561 cgcggcttga agcctgttgg ggcccaatgg tcaattaagg gagttttcgt ggcgcaagcg
     1621 ggcaaatcaa ggaacgttgc gaactcgcgg cggctcattt ggctcgcgct cgcaacggtc
     1681 tccttcctgg caagcgggca cagcgctttt tgccaaggcg ttgtcaaatc caagcatggc
     1741 gactgggatc tgcgctgcga gactcctccc ggcgcggcaa aggagcaatg cgcgctgttg
     1801 cagagcgtcg ccgcagaaga caagccaaat atcaatctta cggtgatcgt gctcaaaacg
     1861 gcggatggaa agaatcggtt gctgcgtgtc attgcccccc tcgggcttct tcttccgtcc
     1921 ggcctcggcc taagaatcga cgatatcgat gtcggccgcg cgggttttgt ccgttgcctt
     1981 ccgaccggtt gcatcgcgga agttgtcatg gaagataaat tgatcgagca aatgaagaac
     2041 ggcaaaaagg ccaccttcat cattttccaa acgcccgagg aagggatcgg cattccgctc
     2101 tccatgacgg gatttaagga aggcttcgat gacttgccgt aaaccgggca ggaaatcagc
     2161 gaggaagcgc tcgctggccc gcggcatcaa ccccttcctt gccgcgcttg tccttttggg
     2221 actggctggc tgcggcggcc atcttgccaa tttcgacgcc cctcaatctc ctgattcgaa
     2281 tggcccagct ggtgccaaac ttcttgctac tatgctgggc atgaaaaaaa acgaggccgc
     2341 gagcgacgta gccgcggcac cgggaccgca ggatcggcat atattttgcc cagaagtcgt
     2401 ccttctcgaa gggactgagg cgagccgggc ttatgccgga acgccggctt caagcgccaa
     2461 tctgcgctat caatttgcgc tcactgaaac ggcccgagaa tgcgcgctcg acgccggtga
     2521 attgaagctt aagattgggg ttgcaggcaa ggtgctgctt ggccccgcag gctctgccgg
     2581 aagttttacg gtgcccgtgc gcatggccat tcttcgcaaa agcgataatg agcccgaagt
     2641 gagcaagctt tatcacgcca aagtaaatgt cggtcaaagc gagacggaag cggatttcac
     2701 gattgtttcc gaaccgattc acgtgccctt catccaagac catagtgagg aagattattc
     2761 gataagggta gggatcgacg agggcccggt gaaggacgaa aagccacgca aaggcgccaa
     2821 accctaacgc gtgccaatcg taccaaaatg taggacaatc tgcgcggcgg ggctgtgcaa
     2881 ccgagccgcg atgccctctt gcatttcata aagctggaca gacgaaaaca atgtcccgca
     2941 cgtgaagatc cgtcacgata aattagtcgc gcgaaagggc cgtggttatg acgattttat
     3001 accgcgatgg gctcttcgcc accttagcac tccttgaatt ctctctcctc aaaagaaagg
     3061 ttgtgcgcac cactatgctg gccgtgatgc tcttatttgt cggaagtggc gtagcggcgg
     3121 cgcagagggg ctcgggtgaa aagctgaaag tcgccttttt cggcttcgag ttggtcaata
     3181 cctcgctgca gcctacgacc gacgccgaaa agcagcgtct tatagttgtg ggcgatacgc
     3241 tgacgcaaat gctggccggc tccggacgct acgaagtcgt gctgctttcg gatgcgttga
     3301 agcagaagat cgagcgaagc gccagggtca cgggctgcaa tggctgccag attgaatggg
     3361 cgcgggaagc gggtgccgat ctcgccgtct ggggcacggt gcagaaggtc agcaacctga
     3421 tcctcaacga gaacgtctat atagatgacg cgaggagcgg gagggagttt ttcagccaaa
     3481 gcgtcgatat ccgcggcaat acggacgaat cctgggtgcg cgggatgcgt tatctcctgc
     3541 gctataagct acttgagaag cgctgagcga ccgctagcac aaggtgccgc cgtccggtat
     3601 gtggtccaag atttctcgct ggcctctgga ttgagcgagt tctgtgtaac tgcgcgcgct
     3661 gcgtcttgcc acttgatgaa tcccaccggg aagggcaaac tgcgaggcga gagttaaagc
     3721 aacagagcca acgcggccag ccgctcacgg catcaaattt ttcgaaaagg aatctgtgat
     3781 tttttgacct caataagctt accagacttc tgggacaaaa aatgtcgttg caatatccac
     3841 ctcattggga tgagccggag caagccatat ttctttaagc aagtctttag caagcgcgtt
     3901 cgcgttgtac ggcatcaaga tatatggaag catgtagggt aatttctttt catcaaatct
     3961 cacatgggtt gtattgtccc ttggaaccag cgcaaacaac cgccactcgc tttcatgggc
     4021 aaacgcacca tgtttaaagg acagagctgc aacgattatg tagtgcatgg tattcagcct
     4081 cgttcaactc ctctggtctt ccgccgttgg gatagtgctg tttaagtaaa ttactaatgt
     4141 tatttatata atttgtgata cctcgcttca ac
//
LOCUS       DATMPU010000108         2604 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_1012803,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000108 DATMPU010000000
VERSION     DATMPU010000108.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 2604)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2604)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2604
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1012803"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..574
                     /locus_tag="VNY06_02495"
     CDS             <1..574
                     /locus_tag="VNY06_02495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX13738.1"
                     /translation="SLLKGKSAVAQSTPVSTEGDPAAIGGPGLSGSIGLGLEIANIAA
                     LPDAVDYAQDEFYCANFTPSEIAHSIKEPVAKAAFQSLLAAKRAIVKSGAVSEPQEGY
                     KNIEISFDGEGRPCYPGCLLSLSDTGTMAAAACFWPAAQPATAAEIARAEQRIIPTRI
                     RIVALLVVLSLLVLFGIGFWKILDFLLPHR"
     gene            755..2122
                     /locus_tag="VNY06_02500"
     CDS             755..2122
                     /locus_tag="VNY06_02500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010682606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HWX13739.1"
                     /translation="MQDEEADRFSARAARYARVGANVGGVAARIASARLIGRAASGNT
                     SAAALTQALGTLKGPVMKVAQMMATVPDLLPPEYAEELQKLQNEAPPMGAAFVRRRMQ
                     AELGTDWLSRFSSFDLRPAAAASLGQVHRATANDGTALACKLQYPDMKSAVEADLRQL
                     DVVFALHRRLNSVIDTREMAKEIGERVREELDYRREAKHAALYRDILVNVEDVRVPGV
                     LPELSTGRLLTMQWLEGRRLLDFKGARESVRNAIAKAIFKAWWIPFSRFGVIHGDAHL
                     GNYAVFGMGETEDAGGINLFDYGCIRIFPSKFVGGVVDLYEGLLHKDFDRTVHAYETW
                     GFRGLTKDLAETLNIWARFVYAPLLDDRVRSVADGVSPAHYGRKEAFTVHQALKEKGP
                     VLVPREFVFMDRAAIGLGSAFLHLDAKLNFYQLFHETMGKFSDAALRERQARALARAG
                     LAQPA"
     gene            2341..2499
                     /locus_tag="VNY06_02505"
     CDS             2341..2499
                     /locus_tag="VNY06_02505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020173396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aa3-type cytochrome c oxidase subunit IV"
                     /protein_id="HWX13740.1"
                     /translation="MAATAENEPQPAGHPGMDYDEHEKTYRMFLHLIKYSVAGVTAIL
                     ALLAFLWG"
BASE COUNT          524 a          731 c          789 g          560 t
ORIGIN      
        1 cagtcttttg aagggcaaga gcgcggtggc gcaaagcact cccgtttcga cagaaggaga
       61 tccggcagcg attggcggcc ccggtctttc tgggtcgata ggtttgggtc ttgagatcgc
      121 aaatatcgcg gctctgccag acgctgtgga ttatgcgcag gacgagttct actgcgccaa
      181 tttcacaccc tccgagatcg cccatagcat caaagagccc gtggcaaaag ccgcgtttca
      241 aagccttctc gcggccaagc gtgctatcgt aaagtcgggc gcggtgagcg agcctcagga
      301 agggtataag aacatcgaga tcagctttga tggcgaagga aggccttgtt accccggctg
      361 cctgctctct ctaagtgaca caggcacaat ggctgccgca gcctgtttct ggccggcggc
      421 gcagcccgcg acggcagcag aaatcgctcg tgcggaacaa agaatcatcc cgacccgaat
      481 caggattgtc gctcttctcg tggtgctttc gcttttggtt ttgttcggaa taggattttg
      541 gaagattctc gattttttgc tcccgcaccg ctaaagtttc gctgggcagc aagcttcgcg
      601 ttccgccgcg ggacccgcta caagcctctg tgaagaggtg gattttgctt gttgctccac
      661 tttattcttc ttagaggaag atgtttcccc ttagaggaaa gtgcggcctt ggcgcataag
      721 cgggccgcca ttccattgag atcgagtcga acgcatgcaa gacgaggaag ccgaccggtt
      781 ttccgccaga gcagcgcgtt atgcccgcgt cggggctaat gtcggcggcg tggccgcgag
      841 gattgcgagc gcccgcctta ttgggagagc cgcatcgggc aacaccagtg cggcggcttt
      901 gacccaagcg cttggcacgc tcaaaggacc tgtgatgaag gtcgcccaaa tgatggcgac
      961 tgtcccggat cttttgccgc cggaatatgc ggaggaactg caaaagctgc aaaacgaggc
     1021 gccgccgatg ggcgcggcct ttgtcagacg ccgcatgcag gcggaactcg gaacagactg
     1081 gctatcgcgt ttttcatcct tcgaccttcg tccggcggca gcggcttcgc tcggccaggt
     1141 ccaccgggca acggccaatg atgggactgc gcttgcttgc aagctgcaat atcccgacat
     1201 gaaatctgcc gtcgaagccg atctgcggca gctcgacgtg gtgttcgcgc tgcaccggcg
     1261 tctcaattcg gtcattgaca cgcgcgaaat ggcgaaggaa atcggcgagc gcgtgcgtga
     1321 ggaattggat taccgccgcg aagccaagca cgccgcgctc tatcgcgaca ttctcgtcaa
     1381 tgtcgaggac gtgcgtgttc ccggcgtgct gcctgagctc tcgaccggcc ggctcctgac
     1441 catgcaatgg ctcgaggggc gcaggctcct cgatttcaag ggcgcgagag aatccgtgcg
     1501 gaatgcgatc gccaaggcga tcttcaaggc ctggtggatt cccttcagcc ggtttggcgt
     1561 catccatggc gatgcccatc tcggcaatta cgccgttttc ggtatgggag aaacagagga
     1621 tgctggcggg ataaacctct tcgattacgg ttgcatccgt atttttcctt cgaaattcgt
     1681 tggcggtgtt gtcgatctct acgagggact gttgcacaag gattttgacc gtaccgttca
     1741 cgcctatgaa acatgggggt ttcgcgggct gaccaaggat ctcgccgaaa ctttgaatat
     1801 atgggcgcgc ttcgtttatg cgcctttgct ggacgaccgg gtgcgcagcg tcgcggatgg
     1861 cgtctcgccg gcccattacg gacgcaagga agcgtttacg gtccaccagg ccttgaagga
     1921 aaaggggcct gtcctggtgc ctcgggagtt cgtcttcatg gatcgcgcgg cgatcggcct
     1981 tggcagcgcc tttctgcatc tcgacgcgaa attgaatttt taccaattgt tccatgagac
     2041 tatgggcaaa ttctcggatg ctgccttgcg ggagcggcag gcgcgcgcgc ttgcaagggc
     2101 cggactggcg caacccgctt agccaggccg ggggcgtatc gctgcgatgc caaaaccgat
     2161 ggcggtcaga gctccatgca gcgatttgcc acggaacaag agctatccga gaaggcgagg
     2221 tgccgcgaag gcagctgccg ctctgcacaa aatttggcaa gcgccgcgct tttccctctt
     2281 gcgcgcggcg aaaatcattg caaaagttaa ggttgaacca gacccggcga tcaaaatgag
     2341 gtggccgcaa cggcggaaaa cgagcctcaa cccgcgggcc atcccggcat ggactatgac
     2401 gaacacgaaa aaacctatcg gatgttcctt cacctgatca aatattccgt cgcgggcgtg
     2461 accgctatcc tcgctttgct ggcctttctc tggggctgat attcccatta caaagcgagc
     2521 cattccctgt ggccaggctt ggccggcgga caagaatcgg gctgggtcac agcttcaagc
     2581 attcccggag ctattcatgc taat
//
LOCUS       DATMPU010000109         9909 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_1944651,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000109 DATMPU010000000
VERSION     DATMPU010000109.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 9909)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9909)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9909
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1944651"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..349
                     /locus_tag="VNY06_02510"
     CDS             <1..349
                     /locus_tag="VNY06_02510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019401561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HWX13741.1"
                     /translation="IHQEPAFAELFIAHLLGRTIRVEADLVDQLFNSSEKRLARLLLL
                     LANFGKEDNPEPIIAKISQETLAEMIGTTRSRVSFFMNKFRKLGFIDYNGDIQVHSSL
                     LNLVLHEEPHIET"
     gene            723..968
                     /locus_tag="VNY06_02515"
                     /pseudo
     CDS             723..968
                     /locus_tag="VNY06_02515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012385361.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
     gene            993..2179
                     /locus_tag="VNY06_02520"
                     /pseudo
     CDS             993..2179
                     /locus_tag="VNY06_02520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012334124.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
     gene            2255..2905
                     /gene="nuoE"
                     /locus_tag="VNY06_02525"
     CDS             2255..2905
                     /gene="nuoE"
                     /locus_tag="VNY06_02525"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020175204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HWX13742.1"
                     /translation="MSVRRLAEKQPKDFAFTAEGEAKCKEILKKYPEGRQASAVVALL
                     WLAQKQNDFWLPKAAIEKVAQRLGMPFIRVLEIATFYTMFNLSPVGKFHVQMCGTTPC
                     VLAGSDAIKKILQGRIGDQGTVTADGKLSWIEVECLGACCNAPMVQINEDYYEDLTAE
                     KFEKLLDDLAAGRAVRIGSQTGRVSSEPVGGLTSLASLYGADGRSGPMSAADISQS"
     gene            2915..4225
                     /gene="nuoF"
                     /locus_tag="VNY06_02530"
     CDS             2915..4225
                     /gene="nuoF"
                     /locus_tag="VNY06_02530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016847754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HWX13743.1"
                     /translation="MLEDKDRIFTNLYGFGDWRLKGAIARGAWDNTKKLIEKDREWII
                     NEVKASGLRGRGGAGFPTGLKWSFMPKPDPARPSYLVLNADESEPGTCKDREIMRHDP
                     QLLIEGCFLACFAMQAHVCYIYIRGEYILEGWRLEAAIAEAYAANLIGKNNIHGWPFD
                     IYVHHGAGAYICGEETALLESLEGKKGMPRLKPPFPANMGLYGSPTTVNNVESIAVVP
                     DILRRGAAWFASFGSKNNAGTKLFCVSGHVNRPCNVEEAMSIPFRELIERHCGGIRGG
                     FENLLAVIPGGSSVPCIPAHEIIDAAMDFDTLRDLKSGLGTAAVIVMDKSTDIVRAIA
                     RISYFYMHESCGQCTPCREGTGWLWRVVSRMAEGRAHKREIDMLLEVTTQIEGHTICA
                     LGDAAAWPVQGLIRHFRPEIERRIDQYAANPHPEPVRDFGVAAE"
     gene            4481..6544
                     /gene="nuoG"
                     /locus_tag="VNY06_02535"
     CDS             4481..6544
                     /gene="nuoG"
                     /locus_tag="VNY06_02535"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012591909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006120 - mitochondrial electron transport,
                     NADH to ubiquinone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HWX13744.1"
                     /translation="MSKLIVDDVEVDVPPEYTLLQACEEAGAEIPRFCFHERLSIAGN
                     CRMCLVEVKGGPPKPSASCAVAVKDLRPGPNGEPPVVLTKSPMAKKARQGVMEFLLIN
                     HPLDCPICDQGGECDLQDQAMAFGVDASRYAENKRAVEDKYIGPLVRTSMNRCIHCTR
                     CVRFMAEVAGTGEMGATGRGEDMEITTYLERAMGSELQGNIADLCPVGALLPKPYSFK
                     ARSWELVKTESIDVMDALGSAIRIDTRGREVMRILPRINEAVNEEWISDKAREIVDGL
                     KTKRLDRPFVRENGQMREASWQTAFEAIAVKVRQSSPQRIGAIAGDLCAVEEMFALKL
                     LIQSLGSASLDCRQDGAKLDPRLGRASYIFNPAIAGIDSADGLIIIGSNPRKEAPVLN
                     ARIRKRWLKGGFPIAVIGERADLTYEYKYLGAGTQALVALAEEPPGSIERPIFLIGQG
                     ALARQDGHAILALAARAAAAHGALKPGWSGFAILHTAAARVGGLDLRFVPGQKGLDAE
                     AMAKRGALDVLFLLGADEIAVEPGAFVIYQGTHGDRGAARADVILPGATYTEKSGIYV
                     NTEGRVQLAERANFPPGDAREDWAILRALSDVLGHKLPFDSLNALRKKLYEAHPHFGK
                     RDEIAAAEPLDLARLTAAGVMLASAPFASPITDFYMTNPVARASAVMAECSAVAGGFA
                     REAAE"
     gene            6550..7578
                     /gene="nuoH"
                     /locus_tag="VNY06_02540"
     CDS             6550..7578
                     /gene="nuoH"
                     /locus_tag="VNY06_02540"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012385355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HWX13745.1"
                     /translation="MTSSAWIGPLFLTLVKSVAFLVVLLIFIAYVLYADRKIWAAVQL
                     RRGPNVVGPWGLWQSFADMLKFVFKEPVIPDGANKGVFLLAPFVMGMLALSAWAVIPV
                     SEGWAIADLNVGVLYIFAISSLSVYGIIMGGWASNSKYPFLSALRSAAQMISYEVSIG
                     FVFITVLLCAGSLNLGDIVAAQDTRFGLLGWYWLPLFPMFAIFFISALAETNRPPFDL
                     VEAESELVAGFMVEYSSTPYMLFMLAEYVNIITMSALTTVLFLGGWLSPLPFFPLTMV
                     PGVIWFILKVCLVFFMVSMAKAIVPRYRYDQLMQLGWKVFLPISLGMVILVAGVLQFT
                     GWGANMGH"
     gene            7604..8092
                     /gene="nuoI"
                     /locus_tag="VNY06_02545"
     CDS             7604..8092
                     /gene="nuoI"
                     /locus_tag="VNY06_02545"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020185822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HWX13746.1"
                     /translation="MKLDHAARAVFLSEFVGAFLLAMRYFFKPKATLNYPHEKNPQSP
                     RYRGEHALRRYPNGEERCIACKLCEAICPAQAITIEAGPRRNDGTRRTTRYDIDMVKC
                     IYCGFCQEACPVDAIVEGPNSEFATETREELYFDKDRLLENGDRWEREIARNLALDAP
                     YR"
     gene            8158..8766
                     /locus_tag="VNY06_02550"
     CDS             8158..8766
                     /locus_tag="VNY06_02550"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013652821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HWX13747.1"
                     /translation="MSIASFFFYLFSSVMIAAASMVISSRNPVHSVLFLILAFFNAAG
                     LFVLLGAEFLAMILVVVYVGAVAVLFLFVVMMLDVDFAELKQGFSQYLTVGASLGLVV
                     LIEIALVVGAWTIGPVSIANVTTPMPPGMTNTMALGHVLYTQYAYLFEAAGLILLTAM
                     IGAIVLTLRHKAGVKRQNIALQNARTPAEAVEIRKVTSRQGV"
     gene            8813..9121
                     /gene="nuoK"
                     /locus_tag="VNY06_02555"
     CDS             8813..9121
                     /gene="nuoK"
                     /locus_tag="VNY06_02555"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011510679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HWX13748.1"
                     /translation="MTVSLAQFLIVAAILFTLGTAGVILNRKNIIVVLMSIELILLSV
                     NINLVAFSAFLGDLTGQVFALFILTVAAAESAIGLAILVAYYRNRGSIAIEDINLMKG
                     "
     gene            9129..>9909
                     /locus_tag="VNY06_02560"
     CDS             9129..>9909
                     /locus_tag="VNY06_02560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012252753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HWX13749.1"
                     /translation="MYAAIVFLPLLGFLIAGLFGWRIGDRPSEFVTTGLLFVSGLLSW
                     VVFFQVALGTAPASVSVLGNWLTSGALQVDWSLRIDSLTAVMLVVVTTVSSLVHLYSI
                     GYMADDPCRPRFFAYLSLFTFAMLMLVTADNLVQMFFGWEGVGLLSYLLIGFWYEKPS
                     ANAAAIKAFVVTRVGDFGFALGIFLVFVLTQSVGFEQIFAAAPALAHHTIHVFGLDLD
                     AMTIASLLLFMGAMGKSAQFLLHTWLPDAMEDRPRNRTERQA"
BASE COUNT         1923 a         2708 c         2925 g         2353 t
ORIGIN      
        1 gatccatcag gaaccagcgt tcgccgagct gttcattgcc catcttcttg gccggaccat
       61 ccgcgtcgaa gccgaccttg tcgatcagct gttcaattcg agtgagaaac gtcttgcccg
      121 tttgctcctg ctgctggcga attttggcaa ggaagacaat ccggagccga taatcgcgaa
      181 aatcagccag gagacgcttg cggagatgat cggcacgacc cggtctcgtg tgagtttttt
      241 catgaacaaa tttcgaaaac tgggcttcat tgactacaac ggcgacatcc aggtccacag
      301 ttcattgttg aatctcgttc ttcacgagga gccgcacatc gagacttaaa tgtgctctgt
      361 tatgctggcg cgtgtggcat gcagctccgc ccgcttgaaa tgatatgtgg gcaatttact
      421 tccccggcac acaggggcag cttaaaacag cgccttaatt cggtgacgct ttctgaccca
      481 tctttttctc aagatccgaa atctcttctg gcgttgccgg acggccagtc aatctctgat
      541 gggccgcgct atagattgtt gtgcgggtgt ccatgggctc gccgggcctc agcaagaagc
      601 ccggcagcat gccgctcgtg cgcacgccat gggcgcggtc gtaggggtgt tcgcggttcc
      661 agccctggtt ggatggtttg ttctcgtaat agacgacgag ggaccggcgc agcggcggga
      721 cttttgcagg ccatccagac ttgcggcgcc tattgactga ttacggcttc gacggccatc
      781 ccttgcgcaa ggatttcccg atgacgggtt atgtcgaggt gcgctacgat gaccagcaga
      841 agcgtgtcgt ctatgagccg gttcagctca cccaggaatt ccgctcgttc gatttcctgt
      901 cgccctggga gggcgcgatc gactatgtct tgcctggcga cgagaaggcc aagggcaacc
      961 cgcaatgacg aacgcctcaa gactgattta acatgagcga caatccagtc cgcactttca
     1021 cgatcaattt tggcccgcag catccggccg cgcatggcgt cttgcggttg gttctcgacc
     1081 tcgacggtga agtcgtcgag cgcgtcgacc cgcatatcgg gttcctccat cgcggcaccg
     1141 agaagctgat ggaggcgcgc ccctatttgc agaatgtgcc ttatttcgac cggctcgatt
     1201 atgtcgcgcc gatgaatcag gaacatgcct tctgccttgc catcgagaaa ttgctcggcc
     1261 ttgaagtccc gcggcgcggc cagcttctgc gggttttgtt ttgcgagatt ggccggattc
     1321 tgtcgcatct tctctgtgtc acgacgcagg ctatggacgt cggtgccttg acgccgccgt
     1381 tatggggctt cgaggaacgc gaaaaactca tggttttcta cgagcgcgcg tcccgcgcct
     1441 tcacgccaat tatttccggc ccggaggcgt gcatatggac gtaccgcaac agctgatcga
     1501 cgacatcggg gcctggtgtg acccgttcct aaaagtctgc gacgatctgc aggctctgtt
     1561 tatcgaaaac cgcatattca agcaacgcaa tgtctatatc ggtgttgtga gtttggatga
     1621 ttgctggcgc tggggatttt cgggttgcat ggtgcgcggc tcgggcgcgc cttgggattt
     1681 gcgcaaggcg cagccctatg aatgctacga ggaaatggat ttcgacattc ccgtaggccg
     1741 acacggcgac aattacgacc gccaagtcat tcgcatggaa gagatgcgcc agtcggtcaa
     1801 gatcatgaaa caatgcatca ataagctttc cgcggcagac ggcaaagggc ccgttgccgc
     1861 taaggatggc aaggtggtgc cgccaacccg cccggagatg aagcgctcga tggaggcttt
     1921 gatccatcat ttcaagcttt atacggaggg cttccatgtg ccggcgggtg aagtctattg
     1981 tgcggtcgag gcgccaaaag gcgagtttgg tgtttacctc ctatcggatg gcaccaataa
     2041 accttaccgc tgcaaaataa gggcaccggg ctttgcgcat ctcgccgcga tggattttct
     2101 ttgccgcaaa tcgatgctcg ccgatgtgag tgccattctg ggttcgctcg atatcgtctt
     2161 cggggagata gacaggtgac aggatctctg cgccgcagga gcaatgacac taaatacgct
     2221 gcccgtgtga ctttgatacc aaagagctaa actgatgtcc gttcgacgtc ttgccgagaa
     2281 acagccaaaa gactttgcct tcaccgctga gggtgaggca aagtgcaagg aaattttaaa
     2341 aaaatatccg gaagggcgcc aggcgtctgc agtagttgcg ttgttgtggc tggcacagaa
     2401 acaaaatgat ttctggctgc caaaagcggc aatcgagaag gtcgcgcaaa ggcttggaat
     2461 gccttttatt cgcgtgcttg agattgcgac cttctacacg atgttcaatc tctcgccggt
     2521 cggcaaattc catgtccaga tgtgcggcac gacgccctgc gtgcttgccg ggtctgacgc
     2581 gataaagaaa attcttcaag ggcgaatcgg cgaccaagga acggtgacag ccgatggcaa
     2641 gctttcctgg attgaggtcg agtgtcttgg cgcttgttgc aatgcgccga tggttcagat
     2701 caacgaggat tattacgaag atctgacggc tgaaaagttc gagaaacttc ttgacgatct
     2761 cgccgcgggc cgcgcggtga ggattggctc gcaaaccggg cgcgtcagct ccgagcccgt
     2821 aggcggactc acctcattgg cctcgcttta tggcgccgac ggcaggagcg gtcccatgag
     2881 cgctgccgat ataagccaat cgtaaggcaa agagatgctc gaagacaagg atcgcatctt
     2941 caccaatctc tacggcttcg gcgattggcg cctcaagggc gcgatcgcgc gcggcgcctg
     3001 ggacaacacg aagaagctga tcgaaaaaga cagggagtgg atcatcaacg aggtcaaggc
     3061 ctcggggctg cgggggcgcg gcggcgcggg atttccgacc ggcctcaaat ggtccttcat
     3121 gccaaagccc gatccggcgc ggccctccta tctcgtcctt aacgccgacg aatccgagcc
     3181 cggcacctgc aaggaccggg agatcatgcg ccatgatccg cagcttctca tcgaaggctg
     3241 ttttttagcc tgcttcgcga tgcaggccca tgtttgctat atctatatcc gtggcgaata
     3301 tatcctcgaa gggtggcgtc ttgaggccgc gattgccgag gcctatgcgg caaatctcat
     3361 cggcaaaaac aacatccacg gctggccatt cgacatttac gtccaccatg gcgcgggcgc
     3421 ctatatctgc ggcgaggaaa ccgcgctttt ggagtcgctc gaaggcaaga aaggcatgcc
     3481 ccggttgaag ccacctttcc ctgccaatat gggcctttat ggctcgccga cgaccgtcaa
     3541 caatgttgag tccatcgccg ttgtgccgga tatcctgcgc cgcggcgccg cttggtttgc
     3601 cagctttggt tcgaagaaca atgcgggtac caagcttttc tgcgtctcag ggcatgtcaa
     3661 taggccgtgc aatgtggaag aggcgatgtc gattcccttc cgcgaactca tcgagcggca
     3721 ttgcggcggg attcgcggcg gctttgagaa tttgctcgcg gtgatccccg gtggctcctc
     3781 ggtaccttgt attccagcac atgaaatcat cgacgcagcg atggattttg acaccctgcg
     3841 ggacttgaag tcaggccttg gcaccgccgc ggtcatcgtg atggacaaat caaccgatat
     3901 cgtgcgcgcc atcgcgcgga tcagttattt ctatatgcat gagagctgcg gccaatgcac
     3961 accgtgccgg gaagggacgg gttggctttg gcgcgtcgtc tcgcgcatgg cggagggccg
     4021 cgcgcacaag cgtgagatcg atatgctgct cgaagtcacg acgcagatcg aaggccatac
     4081 gatttgcgct ctaggggacg cagcggcttg gccagtgcag gggctcatcc gtcattttcg
     4141 gcctgaaatc gagcggcgca tcgatcaata tgcggcaaat ccgcatccgg agccggtgcg
     4201 cgactttggc gtcgcggcag agtagcgaag ggcggacgcg cgttcgtcct gcatattgga
     4261 atattggtgg cgctctgacg cgacgagcgg tcaggctaca aaggacgcag gcgatttgcg
     4321 gattcacagg aaagcgttgg aagagaggct ggcaggaacg aggtggctta cacaaaattg
     4381 ctattctctt ccaaagcaat tcgctgcatc gaccggatcg gaacgcggtg gggtcgggcg
     4441 gcaactcaat cttgccgaag attcgcagtg aggtaacggc atgagcaaac tcatagtgga
     4501 cgacgtcgaa gtcgatgtcc ctccggagta cacgcttctc caggcatgcg aagaagcggg
     4561 tgcggagatt ccacgtttct gctttcatga gcgtctttcc attgcgggca attgccggat
     4621 gtgcctcgtc gaggtgaaag gcgggccgcc caagccttcg gcgtcctgtg ccgtggccgt
     4681 aaaggatctt cgtccgggac cgaatggcga accgcctgtc gtgctcacca aatctccaat
     4741 ggcgaaaaaa gcccgccaag gggtcatgga attcctgctg attaatcatc cgctcgattg
     4801 cccgatatgc gaccaaggcg gcgagtgcga tttgcaggat caggccatgg cattcggcgt
     4861 cgacgcctcg cgctatgccg aaaataaacg cgcggtcgag gacaaatata tcggccctct
     4921 ggtccgcact tcgatgaacc gttgtattca ttgcacccgc tgtgtgaggt tcatggcgga
     4981 agtcgctggc actggggaga tgggcgcgac cgggcgcggc gaggacatgg aaatcacgac
     5041 ctatcttgag agggcgatgg gctcggagct gcaaggcaat attgccgatc tctgcccggt
     5101 tggcgcgtta ttgccaaagc cttattcgtt caaggcgcgg tcgtgggagc tcgtcaagac
     5161 cgagtcgatt gatgtcatgg atgcgcttgg ctcggccata cgtatcgata cgcgagggcg
     5221 ggaggtcatg cgcatcctgc cccgtatcaa tgaagccgtg aatgaggaat ggatttccga
     5281 taaggcacgc gagattgttg atggtctcaa gaccaaaagg cttgatcgcc cttttgtgcg
     5341 cgagaatggc caaatgcgcg aggcctcttg gcagaccgcc ttcgaggcga tcgctgtgaa
     5401 agtacggcaa agttcgccgc agcggatcgg ggcgatcgcc ggcgacctct gcgctgtcga
     5461 ggagatgttt gcgctaaaac tcttgattca aagcctcggt tctgcttcgc tcgattgccg
     5521 gcaggatggc gcaaagctcg atccccgcct cggccgcgca agctatattt tcaatcccgc
     5581 catcgccggt atcgattctg cggatggcct catcatcatc ggctcgaacc cgcgcaagga
     5641 agcacccgta ttgaacgcgc gaattcgcaa gcgctggttg aagggtggat tccccatagc
     5701 ggtcatcggt gagcgcgctg atcttaccta tgagtacaaa tatctcggcg ccgggacgca
     5761 agcgctcgtg gccctcgccg aagagccgcc gggctcaata gaacggccca tctttttgat
     5821 cggccaaggt gcgcttgctc ggcaggatgg tcacgccatt ctggcgcttg ccgcaagggc
     5881 cgcggctgcc catggtgctt tgaagcccgg ctggagcggc tttgctatcc tgcacacggc
     5941 tgcagcgcgc gtcggcggcc tcgatctgag attcgtgccg ggtcaaaagg gacttgatgc
     6001 cgaggccatg gcgaaaaggg gcgcgcttga cgtcctattt ctgcttgggg ccgatgagat
     6061 cgcagtggaa ccaggcgcgt tcgtaatcta tcaagggacg catggcgacc gaggcgccgc
     6121 ccgcgccgat gtcattttgc cgggagcgac ctacacggag aaatctggca tctatgtcaa
     6181 tacggagggc cgcgtgcaac tcgcggagcg cgccaacttt ccacctgggg acgcacgcga
     6241 ggattgggcc attctgcggg ccctttccga cgtgctggga cataaattgc cgttcgattc
     6301 gctgaacgcc ctgcgcaaga agctttatga ggcgcatccg cattttggca agcgcgatga
     6361 gatcgctgcg gccgaacctt tggaccttgc gagactaact gcggccgggg ttatgttggc
     6421 cagcgcgcca tttgcttccc cgatcacaga tttttatatg accaatccgg ttgcgcgcgc
     6481 ctcggcggta atggccgaat gttcggcggt tgccggagga ttcgcgcgcg aggcggcgga
     6541 gtaaagccca tgacctcttc ggcctggatc ggacctctgt ttttgacgct ggtgaaaagc
     6601 gttgcgttcc ttgtcgtgct tttgatcttt attgcctatg ttctttatgc ggaccgcaag
     6661 atatgggcgg ctgtgcaact tcgccgcggc cccaatgtgg ttggtccctg ggggctgtgg
     6721 cagagctttg ccgacatgct gaagtttgtc tttaaggagc cagttatccc cgacggcgcc
     6781 aataaaggcg ttttcttgct cgcgccattc gtgatgggaa tgctggcgct ctcggcctgg
     6841 gcggtcattc cggtgagcga aggttgggca atcgccgatc tcaatgtcgg tgttctctat
     6901 attttcgcga tttcctcgct cagcgtttac ggaatcatta tggggggctg ggcatcgaac
     6961 tccaaatatc cgtttctctc cgcactgcgc tcggcggcgc agatgatctc ttacgaggtc
     7021 tcgatcggct ttgtcttcat cacggttttg ctctgcgcgg gcagcctcaa tctcggcgat
     7081 atcgtcgccg cccaggatac acgctttgga ttgcttggct ggtactggct tccgcttttt
     7141 ccaatgttcg ctatcttttt tatttcagcg cttgccgaga ccaaccgccc gccttttgac
     7201 cttgtcgagg ctgaatcgga gcttgtcgct gggttcatgg tcgaatattc ctccacgccc
     7261 tacatgctct ttatgctcgc cgaatatgtg aacatcatca cgatgagcgc gctgacgaca
     7321 gtcctatttc tgggcggttg gctttcgccc ttgccatttt ttccgctcac aatggttcca
     7381 ggcgtcatct ggttcatttt gaaggtttgc ctcgtttttt tcatggtctc catggccaaa
     7441 gcaatcgtgc caaggtatcg ctacgatcag ctgatgcagc ttggttggaa agtgtttctc
     7501 ccgatctcac ttggcatggt gatccttgtt gcgggcgttc tccagtttac tggctggggc
     7561 gcaaacatgg ggcattgagt gtggcttgca tggatgaacc atcatgaaac ttgaccatgc
     7621 cgcaagagcg gttttcctaa gcgaatttgt tggcgcattc ttgcttgcga tgcgctactt
     7681 tttcaagcct aaggcgacgc tcaattatcc gcatgagaag aatccgcagt cgccgcgcta
     7741 tcgcggtgaa catgcgctgc ggcgctaccc aaatggcgag gagcgctgta tcgcttgcaa
     7801 gctctgcgag gccatttgtc cggcacaggc aatcaccatc gaggccgggc cgcgccgcaa
     7861 tgacggcaca cgccgcacca cgcgctatga catcgatatg gtgaagtgca tttactgcgg
     7921 cttctgtcag gaggcctgtc cggtcgatgc gatcgttgaa gggccaaatt cggaattcgc
     7981 gactgagacg agagaggaac tctatttcga caaggaccgg ctgctggaaa acggcgatcg
     8041 ctgggagcgt gaaatcgcgc gcaatctcgc gctcgacgcg ccctaccgct aggtatttga
     8101 ccacagccga aggatgggtt tgccagacga gcggcgaggg gctagcgagg ttaaccgatg
     8161 agtattgcat cctttttctt ttatctcttc tcgtcggtca tgattgccgc tgcgtccatg
     8221 gtaatttcct cccgcaatcc agttcactcg gtactgtttt tgatcctcgc ctttttcaat
     8281 gccgctgggc tgttcgtttt gcttggggcg gagtttttgg cgatgatcct cgtcgttgtt
     8341 tatgtgggag cggtagccgt tctcttcctt ttcgtcgtga tgatgctcga cgtcgatttt
     8401 gccgagctta aacagggatt ttcgcaatat ctgacggtcg gcgcatcgct cggtctcgtg
     8461 gttttgatcg aaatcgcgct tgtcgtcggg gcttggacga tcggacccgt gtctatcgcc
     8521 aatgtcacga cgcccatgcc gcctggtatg accaacacga tggcactcgg gcacgtgctc
     8581 tatacgcaat atgcctatct gttcgaggcg gcaggactca tactactcac tgcaatgatt
     8641 ggggcaattg ttttaactct tcgccacaag gctggcgtaa agcgccagaa tattgcattg
     8701 cagaacgcaa ggacccctgc cgaggcggta gagatccgca aggtcacatc gaggcaagga
     8761 gtttgaacca ggccgttgca gtccgcgcgt gcggcgtggc aagggaaccg tcatgacagt
     8821 gtcgctcgcc caattcttga ttgtcgccgc cattctgttc acgctcggca cggcgggcgt
     8881 cattttgaac cgcaagaaca tcatcgtagt gctgatgtcg atcgagctga ttttgctttc
     8941 ggtcaacatt aaccttgtcg cgttttccgc tttcctcggt gatctcacag gccaggtttt
     9001 tgcactgttt atcttgacgg tggctgcggc cgagtcggca atcgggcttg ctatacttgt
     9061 agcctattat cgcaaccgcg gttcgatcgc gatcgaagac atcaacttga tgaagggctg
     9121 agccaaagat gtatgcagcc atagtctttc tgccgctctt gggatttctg attgccgggt
     9181 tgttcggctg gcggatcggt gaccggcctt cagaatttgt taccacgggc ctgttgtttg
     9241 tctccggcct cctgtcctgg gtcgtctttt ttcaagttgc gctggggacg gccccggcga
     9301 gcgtttcggt tctcggcaat tggctcacat cgggcgcgct gcaggttgac tggtcgctga
     9361 gaatcgactc gcttaccgcg gtcatgctcg tcgtcgtcac aaccgtctca tcactcgtgc
     9421 atctctattc gatcggctat atggccgacg atccgtgccg gccgcgattc ttcgcctatc
     9481 tctcgttgtt cacttttgcc atgctcatgc tggtaacggc cgacaatctt gtccaaatgt
     9541 tcttcggctg ggaaggcgtg ggcctcctct cctatctgct gatcgggttt tggtatgaga
     9601 agccgtcggc gaatgctgcg gctatcaagg catttgtcgt gacccgcgtc ggcgatttcg
     9661 gctttgctct gggaattttc ctcgtcttcg tccttaccca gtcggttggg ttcgaacaga
     9721 tcttcgcggc ggcacctgct cttgcccacc acacgatcca cgtctttgga cttgacttgg
     9781 acgcgatgac catcgcttca cttcttctct ttatgggcgc gatgggaaaa tcggcgcagt
     9841 tccttctgca tacgtggttg cctgacgcaa tggaagaccg gccgcgcaat cgaacggaaa
     9901 ggcaggcag
//
LOCUS       DATMPU010000110         3133 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_698884,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000110 DATMPU010000000
VERSION     DATMPU010000110.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 3133)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3133)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3133
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_698884"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..346
                     /locus_tag="VNY06_02565"
     CDS             <1..346
                     /locus_tag="VNY06_02565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_260200023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HWX13750.1"
                     /translation="AHDKGRRHDFRLFKTSKVHLHPETKAVVDSGYQGLQRAHANTAI
                     PKNCSKKNPLSATDTARNRATSSERVPCEKVIAMLKCFKIIADRYRNRRRRSSLRFFL
                     IAAIYNIELKTA"
     gene            366..1595
                     /gene="glp"
                     /locus_tag="VNY06_02570"
     CDS             366..1595
                     /gene="glp"
                     /locus_tag="VNY06_02570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003609437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990586 - divisome complex [Evidence
                     IEA];
                     GO_function: GO:0061599 - molybdopterin molybdotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gephyrin-like molybdotransferase Glp"
                     /protein_id="HWX13751.1"
                     /translation="MYNSNELLSVPEARICILASVPKDRSFEDVALEQAAGRTLARDL
                     AAKRTHPPKAVSAMDGYAVRACDLTHLPVKLKQIGESTAGFGFSGSLGPKEAVRIFTG
                     APVPEGCDTILIQEHARVDAGFVEPLETVAAGRHIRARGIDFTTGEVLLPAGLRLGAV
                     SLALAAAMNFAKVTVTRRPLVAILTAGDELVRPGKAIGADQIVATNSYAIAALIEGAG
                     GEPLDLGIANDELAAVEKGISAARASGADLLVTLGGASVGDYDLVKPALARQGMELNF
                     WRIAMRPGKPVIHGRLGPMIILGLPGNPASAIVAGIVFLVPLVRALCGDPDAGRDQSE
                     PAILGKALRANDNRQDFLRARLTSNQAGLPIATPFEEQDSSLLRILAQSQCLVLREPH
                     AKPAVAGDPCRMIRLAF"
     gene            1715..>3133
                     /gene="glpD"
                     /locus_tag="VNY06_02575"
     CDS             1715..>3133
                     /gene="glpD"
                     /locus_tag="VNY06_02575"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: protein motif:HMM:NF008899.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HWX13752.1"
                     /translation="MDTRSSVDLFIIGGGINGAGIARDAAGRGLKVMLCEQGDLASAT
                     SSASSKLIHGGLRYLEYYRFGFVREALREREMLLGIAPHLVWPARFILPHDKSLRPSI
                     VIQAGLLLYDWLAPRKRLDRSRRLDLRTAQEGTPLKQTVATGFAYSDCRVDDSRLVVL
                     NAVDARERGADIRTRTRCISAKRESGVWNATLLDLHGATKTIVTARALVNAGGPWAAE
                     MLTGVIDCNAGQTKLQLVKGSHIIVPRLYEGEQCYVLQNEDRRIVFVMPYETNYSLIG
                     TTDIPFAGDPTTAKISADEIAYLCTAVSHYFREELGPKDVQESFSGVRPLYDDGATNA
                     SAASRDYVLDLDAPSAGAPLLSIFGGKVTTYRRLAEDAIDRLSPYLKPPNAGHWTATT
                     PLPGGNIKGGDFGQFLHRLRGKYPWLDVGTASRLARAYGTKAELILGDAQSARDLGTD
                     FGAGLTKAEIDYLIDQEFALTAE"
BASE COUNT          679 a          906 c          910 g          638 t
ORIGIN      
        1 cgcccatgat aaaggacggc gccacgactt caggctgttc aagacgtcaa aagttcatct
       61 gcatcctgaa acgaaagccg ttgtcgacag cggctatcaa ggcctgcaga gagcgcacgc
      121 caacacggcg ataccgaaga actgcagcaa aaagaatccg ttatccgcaa ccgacacggc
      181 cagaaaccgc gctacctcca gcgaacgcgt gccttgcgag aaggtcatcg ccatgctcaa
      241 atgcttcaag attatcgctg accgctacag aaatcgccgc cgcagatcca gcctgcgctt
      301 cttcctcatc gccgcaatct acaatatcga actcaaaacc gcataaaggt tatgcaagag
      361 gcccaatgta taatagcaac gagctgctat cggtgccaga ggcccggatt tgtattctgg
      421 caagtgttcc aaaggaccgc tcctttgagg acgtggcact ggaacaagcg gccggacgaa
      481 ccctcgctcg ggatctcgcc gccaagcgca cgcacccccc caaggcggtc tccgccatgg
      541 acggctatgc cgtgcgagct tgcgacctga cgcacctccc ggtgaagtta aaacaaatcg
      601 gcgaaagcac tgctgggttt ggattttctg gttcccttgg tcccaaggaa gcggtgcgga
      661 ttttcaccgg tgcgccggtg ccggaggggt gcgacacgat tctgatccag gaacacgccc
      721 gcgttgatgc aggtttcgtc gaaccattgg aaacggtggc agccggccgc catattcggg
      781 cgaggggcat cgactttaca acgggagagg tcttattgcc cgccgggctg cggcttggtg
      841 cagtaagtct ggcgcttgcc gcggcaatga atttcgccaa ggtaacggtt acaaggcgtc
      901 cactggtggc cattcttaca gccggcgacg aattggtgcg gcctgggaag gcaattggcg
      961 cggatcaaat cgtagcgacc aattcctacg ccattgccgc cctcatcgag ggagctggcg
     1021 gcgagccgct cgatcttggt attgccaacg atgagttggc ggccgtcgaa aaaggcataa
     1081 gcgccgcgcg cgcatccggc gctgacctgt tggtgactct tggcggtgca tctgttggtg
     1141 actatgatct tgtaaaaccg gcgctcgccc ggcaagggat ggagctgaat ttttggcgta
     1201 ttgcgatgcg tccaggaaaa cccgtgattc atgggcgact tgggccgatg attatccttg
     1261 gcttaccagg aaatccggct tccgcgatcg tcgcaggaat cgtttttctt gtgccgctcg
     1321 tgcgtgcgct ctgcggggat cccgatgcgg gccgagatca gagtgagccg gcaatcctcg
     1381 gcaaagcgct tcgcgccaac gacaaccgcc aagattttct gcgagcaaga cttacatcca
     1441 atcaagcggg gctaccgatc gcgacacctt tcgaggaaca ggattcttcc ctgttgcgaa
     1501 ttctcgcgca atcgcaatgc cttgttttgc gcgaacctca tgcgaagccc gcggtggcgg
     1561 gtgacccttg ccgcatgata cggcttgctt tctgacataa gaaccgcagg cacatttgcg
     1621 caacgcgacc tcgctgcggt gatcatcact tcaggcaaga attgttgtta gcgctgatga
     1681 gagcggccaa aacgaggatg cttttagcag attgatggac acgcgatcaa gcgtcgacct
     1741 ctttataatt ggaggcggca tcaatggagc cggcatcgcg cgtgatgccg ccgggcgcgg
     1801 gctcaaagtg atgctctgcg agcagggcga tctcgccagc gcgacgtcgt cggcatcgag
     1861 caagctcatt cacggtgggc tgcgttatct agaatattat cgcttcggct tcgtgcggga
     1921 agccctgagg gaacgggaga tgctgctcgg tatcgcgccg cacctcgtgt ggcccgcacg
     1981 cttcatcctg ccgcacgata agagtttacg tccttctatc gtcatccagg caggactttt
     2041 gctttacgat tggctcgcac cgcgtaagcg gttggaccgc tcgcggcgcc ttgatcttcg
     2101 cactgcgcaa gaagggacac cgctcaaaca aaccgtggcg actggctttg cctattcgga
     2161 ctgccgcgtg gatgattcaa ggctggtcgt actgaacgcc gtcgatgccc gcgagcgcgg
     2221 cgcagatatc cgtaccagaa cacgctgcat ttcggcgaag cgcgaaagcg gagtgtggaa
     2281 cgcaacactg ctcgatctgc acggcgccac taaaaccatc gtgactgcgc gcgccctcgt
     2341 taatgctggg ggaccttggg cggccgaaat gctgaccggc gtaatcgact gcaatgccgg
     2401 tcaaacaaaa ctgcagcttg tcaagggaag ccacatcatc gtgcctcgcc tctacgaagg
     2461 agagcaatgc tacgtgctgc aaaacgaaga ccgccgtatc gttttcgtga tgccttatga
     2521 aacgaactat tcgctgattg gcacgaccga tatcccattt gcgggcgatc ccacgacggc
     2581 gaaaataagc gccgatgaaa tcgcttacct ctgcaccgcc gtctcgcatt atttccgcga
     2641 agagttaggc cctaaagatg tgcaggaaag cttttcgggc gtgcgtccgc tttatgacga
     2701 cggcgcaaca aatgcgtcgg ccgccagccg cgactacgtg cttgatctcg acgcgccgtc
     2761 agcgggggcg ccgctacttt cgatatttgg cggaaaggtc accacttacc ggcgtttggc
     2821 ggaagacgcg atcgacagac tgtcaccata tctcaaacca cctaacgcag ggcactggac
     2881 tgcgacgaca ccgctccccg gcggtaacat caaaggcggc gatttcggcc agtttcttca
     2941 ccgactgcgc ggcaaatatc cgtggcttga tgttggaaca gcgtcgcggc tggcccgcgc
     3001 ttatgggacc aaagcggaat taatccttgg cgatgctcaa agcgcccgcg atcttggcac
     3061 ggatttcggt gctggactca caaaggcgga gattgattat ctgatcgacc aggaattcgc
     3121 gctcaccgcc gag
//
LOCUS       DATMPU010000111         3416 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_1630312,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000111 DATMPU010000000
VERSION     DATMPU010000111.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 3416)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3416)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3416
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1630312"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..216)
                     /locus_tag="VNY06_02580"
                     /pseudo
     CDS             complement(1..216)
                     /locus_tag="VNY06_02580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014492775.1"
                     /note="One of the components of the high-affinity
                     ATP-driven potassium transport (or KDP) system, which
                     catalyzes the hydrolysis of ATP coupled with the exchange
                     of hydrogen and potassium ions; incomplete; partial in the
                     middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit B"
     gene            complement(200..706)
                     /locus_tag="VNY06_02585"
                     /pseudo
     CDS             complement(200..706)
                     /locus_tag="VNY06_02585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013529643.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
     gene            complement(719..808)
                     /locus_tag="VNY06_02590"
     CDS             complement(719..808)
                     /locus_tag="VNY06_02590"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0043462 - regulation of ATP-dependent
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit F"
                     /protein_id="HWX13753.1"
                     /translation="MTFDNLLGAIATAVVMIYLVIALIRPERF"
     gene            1252..1515
                     /locus_tag="VNY06_02595"
     CDS             1252..1515
                     /locus_tag="VNY06_02595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX13754.1"
                     /translation="MARLSGAIPPSGAWFSFADRGVPPMLVCSLAQLDDALSLTTTVA
                     EMRADARSGRNGWLRGRVDVLDGEGKFAFVAAVGCRAIRCRDR"
     gene            complement(1496..1690)
                     /locus_tag="VNY06_02600"
                     /pseudo
     CDS             complement(1496..1690)
                     /locus_tag="VNY06_02600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012222646.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS481 family transposase"
     gene            complement(1814..>3416)
                     /locus_tag="VNY06_02605"
     CDS             complement(1814..>3416)
                     /locus_tag="VNY06_02605"
                     /inference="COORDINATES: protein motif:HMM:NF039364.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF6165 family protein"
                     /protein_id="HWX13755.1"
                     /translation="DEAVRLNPAAPDKWLNRGNVLQDLDRNPEAVASYNEALRTKPHY
                     PEALSGLGVALKEAGQIDEALVYFDEALKHKPGYPDARNNRAGALLLKGSLKAGFEDF
                     ESRWDRSNAPPKTLISDLPIWEGQKLAGQAIVVWDEQGLGDLIQFSRYLLCLVDAGAD
                     VTLLCRKNMHRLLRTLPKPIRLLEALDSNESFAFQCALMSLPCGFQTSLETVPAPIPY
                     LHPELGLVAKWAGRIGTERFRIGVGWQGNKLIDLQRSIPLACFAPLAAIEDVRLISLM
                     KDQAAIEVETPAGGRFTIESLGNDFDAGSDSFMDCAAAMASLDLVVTSDTAIAHLAGA
                     MGRPVFLALKRVPDWRWLMHREDCPWYPSMRLFRQAEKGDWEPVFKQIASSIAADVTK
                     KAGQLTGAPRAMTAITIPGAVGELIDKITILELKESHIGEAAKLENIRRELTLLRKLK
                     SECGLGGARLAELQAELKSMNGVLWNIENALRGHESREDFGAVFVSLARQVYRTNDRR
                     ATLKKKINLLFNSIIIEEKSYSITS"
BASE COUNT          713 a          992 c         1015 g          696 t
ORIGIN      
        1 ggtgcgcgcc ttgcgcaagg tcgcggcctg cgccttgcca cgtccctcgg ccacggcttc
       61 cgcgaaattc gcaaaaagca aggtgatcca gagccaaagg cagatctgaa acgtgaattt
      121 cgccgtacct gcttccccac cggtcaggag ctcacgcaag tagatgatcg tcgtcagcac
      181 gctcacgatc tcgacgacga ttgccaatgg ttcgcgtcga gcggcgagca aagcccctga
      241 tgagggcgat ggcgagcgcg atgccggtcg cggcggatac gaaattatgc acggtgagcc
      301 cggccatctg ggtcagatag ctcatcgtcg tctcgggcgt gtaggattgc caattggtgt
      361 tggtgacgaa gctcaccgac gtattgaagg cgagatcggg agagaccgca tcgagatgct
      421 gcgggttgaa gggaaggacc gcctgcaagc gctgcaacgc gtaaagggcg aggaagcccg
      481 ccaaactgaa agtgagcacg gcgaggccgt agctcttcca gtcctgctcc tcatcttcct
      541 tgaccccgct cagcgcatag attgtccgct caaggggccg cagtggaagc gacagaaaag
      601 tgcgctcgcc cttgaagacg cgggtcatat aatagccgaa cggcttcacc agaaggacga
      661 ccaccccgca aaaaagcgcg atctgaacaa aaccattggc tgtcattgca tctctttctc
      721 aaaagcgctc gggtcgtatc aaggcgatga cgagataaat catgacgacc gcggttgcga
      781 tcgcgccaag caagttgtcg aatgtcatgg ctcttccctc acaagcgggc gcaggcgttc
      841 agatagagcg ccgtgacggc aaaggccgcc acgccaatgg cgacaaagac gaagtcgaga
      901 gcgatgctca gcattggagg tcctttccgg cgcgtcgccg gtcatgccca acagtgcttt
      961 cacgagcatc gcgaaagcac cctgttgttt tggcccgtcg cgatttctcg gcgaactgat
     1021 gcctacttcg cctgaaaatg ctccaggcta gcaatgcggg ctcgagtatc gggaacggca
     1081 cgtatagatt ccagggtgct caacggcgcc ggatataaaa attccgtata acccgcagtc
     1141 cccgaatgac gcgctcattt gccgcgcggt tggagcgatt cagctatgat aaatcagcat
     1201 attgcgtgtt gttggtcgag gcggccatgg cgaagccggc ggaggaatcc catggcgagg
     1261 ctctcaggcg cgattccgcc ttccggcgca tggttcagtt ttgcggaccg gggggtaccg
     1321 ccgatgctgg tttgctcgct ggcgcaactc gacgatgcgc tcagccttac tacgacggtg
     1381 gccgagatgc gcgccgacgc gcgctcgggt cggaatggct ggctgcgcgg gagggtcgat
     1441 gttcttgacg gcgagggaaa gtttgcattt gtggcggccg ttgggtgccg cgctattcgg
     1501 tgccgcgatc ggtgagaaca cgctgcgcta cggcgccgtt ctccgcgcgg aagggcaaga
     1561 cccggccgtt tcagagaatg gcggccatga tcggcgtctc gcaatcatag agcttggcga
     1621 agccggcctt ggcataggtg tcgatgaaag tttgccgata gacccggcca acccctttca
     1681 gcgtgcccat acggaggtgt cccgcgccgc aaagcccaag gcataccact ttccggcaat
     1741 ctgaactcta attgccgcgc cgcaagaact cgttggagac ccgcacccga cccaaggcgg
     1801 cgggaaagcc ggttcaactt gttattgaat atgatttctc ctcaataatt atggaattga
     1861 agagcaaatt gatctttttc tttagagtgg ccctacgatc gttggtccta tagacctgcc
     1921 ttgccagcga gacgaagacg gcgccgaagt cttcccttga ttcatgtccc cgcagggcat
     1981 tctcgatgtt ccagagcacg ccattcatgg acttgagttc ggcctggagt tcggctagcc
     2041 gcgcgccgcc gaggccgcat tcgctcttga gcttcctcaa cagcgtgagt tcgcgccgga
     2101 tattttcgag cttcgctgct tccccgatgt gactttcctt gagctccaag atcgtgatct
     2161 tgtcgatgag ctcaccaacc gctccgggga ttgtaattgc cgtcattgcg cgcggcgcgc
     2221 ccgtcaactg gccagctttt ttcgttacat ctgccgcgat gctcgatgcg atctgcttaa
     2281 aaacgggctc ccagtctcct ttttcggctt ggcgaaagag ccgcatgctg ggataccaag
     2341 ggcaatcctc gcggtgcatc agccagcgcc aatccggcac gcgtttcagg gcgagaaaaa
     2401 ccggccttcc catggccccg gcaagatgcg caatcgcagt atccgaggta acgacgaggt
     2461 cgaggctcgc catcgccgcc gcgcagtcca tgaaggaatc ggatccggca tcgaaatcgt
     2521 ttcccaagct ctcgatcgtg aaccgcccgc ccgccggcgt ttccacctcg atggcggctt
     2581 gatccttcat aaggctgatc agccgcacgt cttctatcgc cgcgagcggc gcgaaacaag
     2641 caagcggaat cgacctttga aggtcgatca atttgttgcc ttgccagcct acgccgattc
     2701 ggaaacgttc ggtgccgatt cgcccggccc atttcgcgac gaggcccagt tctggatgaa
     2761 gataggggat tggcgccgga acggtctcca aactggtttg gaaaccgcaa ggaaggctca
     2821 tgagcgcgca ctgaaaagca aaactttcgt tcgaatcgag agcttcgagg aggcgaatcg
     2881 gcttcggcaa ggtgcgcaga agccggtgca tgttcttgcg gcacagcaag gtaacatcgg
     2941 cgccggcgtc aacgagacat agcagataac gcgaaaactg gatcaggtcg ccgagaccct
     3001 gctcatccca tacgacaatt gcttgcccgg ctaatttctg gccctcccat atcggcaaat
     3061 ccgagatgag ggtttttggc ggcgcattcg aacggtccca gcggctttcg aaatcctcga
     3121 agccggcttt caagctaccc ttgaggagga gcgcgccggc ccggttgttt cgcgcgtccg
     3181 gatagccggg cttatgtttc aaggcctcgt cgaaatacac cagagcctcg tcgatctggc
     3241 ctgcctcctt aagagccacg ccaagaccgg acaaggcttc gggataatga ggtttggtac
     3301 gcagggcttc gttatagctg gccacggctt ccggattgcg atcgaggtcc tgcaaaacat
     3361 tgccgcgatt aagccacttg tctggcgccg cgggattaag gcggacggct tcatcg
//
LOCUS       DATMPU010000112         7778 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_1951831,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000112 DATMPU010000000
VERSION     DATMPU010000112.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 7778)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7778)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7778
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1951831"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(157..271)
                     /locus_tag="VNY06_02610"
                     /pseudo
     CDS             complement(157..271)
                     /locus_tag="VNY06_02610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611977.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(414..971)
                     /locus_tag="VNY06_02615"
     CDS             complement(414..971)
                     /locus_tag="VNY06_02615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012589333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HWX13756.1"
                     /translation="MTADLLAVIPNLRAFAVSLCGNIDHADDLVQETLVKAWTNIDSF
                     VAGTNLRAWLFTILRNIYYSEYRKRRREVADPDGAFAANLATAPAQSGHMDMLDFRAA
                     LQQLPSDQREALILIGASGLSYEEAAGICGCAVGTMKSRVNRARNRLAEMLAITSGEE
                     FGHDGTWPATIESPAGQHTERDEGD"
     gene            complement(1046..1198)
                     /locus_tag="VNY06_02620"
     CDS             complement(1046..1198)
                     /locus_tag="VNY06_02620"
                     /inference="COORDINATES: protein motif:HMM:NF037848.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016989 - sigma factor antagonist activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NepR family anti-sigma factor"
                     /protein_id="HWX13757.1"
                     /translation="MSKSDIADQIGVHLRCIYDDVLAQPVPGRFLDLLQQLECNAGSR
                     PSKDTM"
     gene            1182..1358
                     /locus_tag="VNY06_02625"
     CDS             1182..1358
                     /locus_tag="VNY06_02625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX13758.1"
                     /translation="MSDFDTAFLEQGAPPIPVSGSGRPSSCFPPGLSDSFALAALIVG
                     FLGFTDLVTEIPLQ"
     gene            1646..2470
                     /locus_tag="VNY06_02630"
     CDS             1646..2470
                     /locus_tag="VNY06_02630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020173992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWX13759.1"
                     /translation="MLTSQAISSQIPYLRRFSRALTGTQAGGDAYVLETLEAIIADPA
                     ELAKCADPKTALYRLFLKVWRTVPINDHVDQAAFAGDEAGAQRNLEAITLRPRIAFLL
                     SALEGFDTSQVAEALDCTEDEAASLIDAAGKEIANQIRTDVLIIEDEPFIALDLQSLV
                     EEQGHRVVDIARTHREATEAVQRQKPGLILADIQLADGSSGLEAVNQILESVSIPVIF
                     ITAYPERFLTGTPPEPAFLVTKPFNIDSLKAVISQALFFDRRSHRKDEPEKSDSLV"
     gene            2697..2867
                     /locus_tag="VNY06_02635"
     CDS             2697..2867
                     /locus_tag="VNY06_02635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX13760.1"
                     /translation="MNKRKEQALILLEAAQGVTIDTEAWLGKADTDNLYVLIVSLILA
                     FIAFVILMRRRF"
     gene            complement(2842..3915)
                     /locus_tag="VNY06_02640"
     CDS             complement(2842..3915)
                     /locus_tag="VNY06_02640"
                     /inference="COORDINATES: protein motif:HMM:TIGR01840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHB depolymerase family esterase"
                     /protein_id="HWX13761.1"
                     /translation="MRLKLPVSGSIGNARSRRSDLADVKTSGEYQRMYAVAEFGSNPG
                     NLAMFACVPRGAAKPSALVVALHGCQQTAREFDLATGWSALGREHGFAVLFPEQKCGN
                     NPQNCFNWFVEKHIRPNDGEIASIRSMIEKLLRDYKLDCGRVFVTGLSAGGAMACALL
                     ASCPDLFAAGAIIAGLPYGAALGPIDAMRAMAFGPEHSARELGNLVRRAAPKQTKWPK
                     ISIWHGSRDEVVAPVNASALVRQWLDIHQIDEAGVVLDEVDGQMRRSWRDGAGTIFVE
                     HYAIDGMAHGVPIDARASLVPRALAAPFALDAGISSTVRIAKSWDLLQHPGSEEGGVR
                     PSRNGEDWFHLFESWFKSAGASR"
     gene            complement(4095..5555)
                     /locus_tag="VNY06_02645"
     CDS             complement(4095..5555)
                     /locus_tag="VNY06_02645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012590425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HWE histidine kinase domain-containing protein"
                     /protein_id="HWX13762.1"
                     /translation="MAPPTKPDFEAVFDCLPTPLLVMDRDLTIIALNRAFLQATKTSR
                     EAVVGNNILTAFPAAQESRGILQQSLERARDQGVTDVVPLVSYNCARDGCDEEHLWSC
                     AHIPVRDKDGQIAYVLKTAQEISELPSSKGFEGLPSAARMGEFSPQSGNLLVLNQTLL
                     ATVQHLRRLVMQAPSFMCILRGPRLVFELVNIAFSMLAGGRDLIGKSLREGLPECEGQ
                     EYPRILENIFKTGEVHVGRKTRILLQDSPDCEMEEHYIDFVAQPIMDAKGEVTGIFIE
                     GNDVTDHVNTEQRQALLIRELHHRVRNTLATVQGLLTTTANTAASFEEFQESFSGRIA
                     SLAKTHAVITEQLHQSISFQQLLTQELGPYTDDQGLRIRLSGPKVELPSQIAVPLGMA
                     VHELTTNAVRHGALAKDEGRIEVDWDLIDRDGERALLCEWSEFAGPPVTPPSRDGFGS
                     MLLKRGLSQQIRADVKVDFAPEGLRLRMAVPLQFGR"
     gene            5919..>7778
                     /locus_tag="VNY06_02650"
     CDS             5919..>7778
                     /locus_tag="VNY06_02650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003610986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exonuclease H3TH domain-containing
                     protein"
                     /protein_id="HWX13763.1"
                     /translation="MSRQMAEADRPLQAGDHVFLVDGSSFVFRAYFQSIRQDAKYNYR
                     SDQLPTGAVRLFCTKLFQFLREGAAKIKPTHLAIIFDKSENSFRRELYPPYKANRSEP
                     PDDLIPQFPLMRAAVRAFGLTPIEQDRFEADDLIATYACEARGRGADVLIVSADKDLM
                     QLIGPGVAMYDPASGEAGSKGARDERKIGTEEVREYFGVPPEKVVDVQALAGDSTDNV
                     PGARGIGLKTAAQLINEYGDLESLLARANEIKQPKRRENLTDPDSVTHIRISKQLVSL
                     VRDVPLEVPIDDLALHAPDGKTLVAFLKAMEFSMITKRAAEAFGVTADLIEPDPEFVG
                     AAGWRGSDRAGPAIAAGEADKSPSANMSRGAPKRTARYGQQTLVTPSATGPNHLVEAR
                     ASAARKEKLDTKSYETISRFEELDAYIAAALETGAISIDTETSTLQTIQAELIGISLC
                     LAPGRACYIPLLHRAEISELFSSSDLQPGQLPETEVIARLKPLLEDASVLKIAQNMKF
                     NWLIFKQRGVTVRPVEDTMLLSYVLDAGRTDHGMDALSENHLGHTPIKISDVAGSGRS
                     LIGFSRAAIDRATEYSAECADITLRLWRVLKPRLPAEHMTNVYETLERPMIEVL"
BASE COUNT         1722 a         2288 c         2106 g         1662 t
ORIGIN      
        1 tttggcgggg caaaggcttc ggcaaagggc ctggtcttgg cccccttttt accccgcgcc
       61 gaacggtgga cttaatggcg gtgctatcga tagcgttctc gtaaggtgtg ccgacaatct
      121 ggcttatctc attgaacatc ctttgccacc ctgctgcgac cagcgatgcc agcggttata
      181 gatggccgta cgctttgggg caatccggcc agcgcatgcc cgattgcagc ccatcgagaa
      241 tcccgctgat cacgaggcga tcatcgaccc gccgtggccc cacgccgttt ccggcccggt
      301 aagacagaga atgatcacgc gccatttaat gcttcgttaa atttgagtgc agacccttaa
      361 cagctcaatt gcatgggaat ttgcggcgca agacgttatt cgcgatgcca atgtcagtca
      421 ccctcgtctc gctcagtatg ttgtccagcg ggcgactcaa tagttgccgg ccaagttccg
      481 tcatgcccaa actcttcccc cgaggtaatc gctagcattt cggcaagccg gtttctggcg
      541 cgattgaccc ggctcttcat ggtgccgacg gcacagccgc aaatgccagc cgcttcttca
      601 taggagagcc ccgaagcacc gatcagaatt agagcttcgc gctgatcgct aggcaactgc
      661 tgaagcgctg cacgaaagtc gagcatatcc atatggccgc tctgcgctgg agctgtggcg
      721 aggttagcgg caaatgcgcc gtcgggatca gcaacttcgc gccggcgttt gcgatattcc
      781 gagtaataaa tattgcgtag aatcgtaaag agccaggcgc ggagattggt ccccgcgaca
      841 aaggagtcga tattggtcca ggccttcacc aaggtttcct gaacgagatc gtcggcgtga
      901 tcaatattgc cgcaaaggga caccgcgaag gcacgcaaat tggggatgac cgcaagaagg
      961 tccgccgtaa tctgcgagct tgcctcacgt ccccgcgttg gctcgcccaa aggcagactg
     1021 cccgcccttt ccttttgcgc cacgctcaca tggtgtcctt gctgggtcta gagcccgcgt
     1081 tgcattcaag ctgctgcaaa agatcaagga agcgccctgg tacgggctgc gccaaaacgt
     1141 catcatagat gcagcgcaaa tgcacgccta tttgatcggc aatgtcggac tttgacaccg
     1201 ccttcctcga gcagggcgcg cctcctatcc cggtttcggg gtccgggcgc cctagctcat
     1261 gcttcccccc ggggctctca gatagtttcg cgcttgccgc cttgattgtt gggtttctcg
     1321 gctttaccga tctcgtcacg gaaatccccc tgcaatgagg ctattctcga cttcatgccc
     1381 atcgccgact ttccagggct gcgaggaaaa ttttttcggc gtcggacccg cgccacatct
     1441 gctccaggat tgccggcgga ttcgaccccc tccctttccc gtgcgcttgg ctcgcggcgt
     1501 aaaaggatta taagcggtaa gtctgaacgt ctggcaagag cgatagttcc gtatctagga
     1561 actttccggt catttgggat tttagccttg ctggatcgac cgagcataat atcaattcaa
     1621 tcgtggaaga aaagtggtgg cctcgatgct aacatcccag gcaatttcgt cgcaaattcc
     1681 ctatttgcgc cggttttccc gtgccctgac ggggacccag gctggaggag acgcatatgt
     1741 tcttgaaacg ctggaagcca tcatcgccga tccggcagag cttgcgaaat gcgcggatcc
     1801 gaagacagcc ctgtaccgct tattcctcaa ggtttggcgt acagtcccga taaacgatca
     1861 tgtcgatcag gcggctttcg cgggggacga agccggtgcg cagcgcaatc tcgaagcaat
     1921 tactttgcgt ccacgtatcg cgttccttct cagtgcgctt gagggcttcg atacgtctca
     1981 ggttgcggag gcgctggatt gcaccgagga tgaagctgcc tctcttattg acgccgcggg
     2041 caaagagatc gccaatcaaa ttcgcaccga cgttttaatc atcgaggacg aaccttttat
     2101 tgcccttgat cttcagtcgc tcgtcgagga gcaagggcac cgggtcgtcg atatcgcgcg
     2161 tacccatcgc gaggcgaccg aagcggttca gcgccagaag ccgggcctca tactcgcgga
     2221 tattcagctt gcggacggaa gctcgggact cgaagccgtg aatcagatcc tcgaatctgt
     2281 ctccataccg gtgatattca ttactgcata tccagaacga ttccttaccg gaacgccgcc
     2341 agagcctgct tttctcgtaa ccaaaccgtt taatatagat agtctgaagg cggtcattag
     2401 tcaggcactc ttctttgatc gccgatcgca tcgcaaggat gagcccgaaa agagcgatag
     2461 cctcgtttaa aggtcctccc gcccaccttg ctggagcgtg ccatttggcc tcgatcattt
     2521 cggggataat agtggaaagc tcgcctcgcg gcgccagcgg cgatgatgtg tctcggcgca
     2581 acggccgtgg ccgcgcgacg gaatccgcgc gtctcgcgtt tcgggtaggg cggccgggtt
     2641 gtcgagcgtg tatccacaac tgagccctgc ctcgccaagc gtgcttccgt gcttagttga
     2701 acaaacgaaa ggaacaagcc ctgatcctgc tagaggctgc gcaaggggtc acaatcgaca
     2761 ccgaagcttg gctcggcaaa gcggacacgg acaacctcta cgttctgatt gtcagcctga
     2821 ttcttgcttt cattgcgttc gttatcttga tgcgccggcg cttttgaacc aggattcgaa
     2881 aaggtgaaac cagtcctcgc cgttgcgcga ggggcgcact ccgccttcct cgctgcctgg
     2941 atgctgcaag aggtcccatg atttcgcgat gcggacagta gaagaaattc cggcatcgag
     3001 agcgaaaggg gcagccaagg ctctgggaac gagggaagcc ctcgcgtcga ttggcacacc
     3061 atgagccatc ccatcaatgg cataatgctc gacaaaaatc gttcccgccc cgtcgcgcca
     3121 tgaccggcgc atttgaccat ccacctcatc gagcaccacg cctgcttcgt cgatttgatg
     3181 gatatcgagc cattgccgga ccagggccga cgcattgact ggtgcgacca cttcatcgcg
     3241 gctcccgtgc caaatggaaa tcttgggcca tttcgtttgt ttaggcgctg cccggcgcac
     3301 gagatttccc agttcccgcg cagagtgctc agggccaaag gccatcgctc gcattgcgtc
     3361 aatgggccca agtgccgctc cataggggag gcccgcgatg atcgctcctg cagcgaacaa
     3421 atcggggcag gaggcaagaa gcgcgcatgc catggccccg ccggcagaaa gacccgttac
     3481 aaagacgcgt ccgcaatcga gtttgtagtc gcggagcaac ttctcgatca tgctccgaat
     3541 cgacgcgatc tctccatcat tggggcggat gtgtttctca acgaaccaat tgaagcaatt
     3601 ctgcggattg ttgccgcatt tctgctctgg aaacaggaca gcaaacccat gctcccggcc
     3661 gagcgccgac cagcccgttg ccaaatcaaa ctccctcgcg gtttgctgac agccatgaag
     3721 ggccacaacc agcgccgaag gctttgccgc gccgcgcgga acgcaagcga acatcgcgag
     3781 attacccgga ttcgacccaa actccgcgac tgcatacatc ctttgatatt ctccggacgt
     3841 cttgacatct gccaaatcgc ttcgccgcga gcgagcattg ccgatgctgc ccgagacagg
     3901 cagctttagt ctcacctaaa ttggttttca attcgacact atcgaaaaat atacttggca
     3961 ggtgaggcgt tacaattaac gtcctgccac caaagttagt cgcatttcac tgatgcatcc
     4021 cggcccggtc atcgtggcag acgcatgggc aacatgtcgc aggggcagga ttgggcggcg
     4081 ccttagcgcc ggcctcaccg cccgaattgc agcggcacgg ccatgcgaag ccttagtccc
     4141 tcgggcgcga aatcaacttt aacgtcggcc cggatctgct gcgacagacc ccgtttcagg
     4201 agcatcgatc caaaaccatc gcgagacggc ggtgtcacgg gagggccagc gaattcgctc
     4261 cactcacaca acagcgcacg ttccccgtcc ctgtcgatca agtcccagtc gacctcgatc
     4321 ctgccttcgt ctttagcaag cgcaccatgc ctcacggcgt tcgttgtcag ttcatggacc
     4381 gccattccga gcggcaccgc gatctgcgat ggcagctcga ctttaggccc agagagacgg
     4441 atccggaggc cctgatcgtc ggtatagggg ccaagctcct gagtcagcaa ttgctggaac
     4501 gaaattgatt ggtggagctg ctcagtgatg acggcatggg tcttggcgag cgacgcgatc
     4561 cgcccagaaa aagattcctg aaactcttca aaactcgccg cggtattcgc tgtggtggtc
     4621 aaaagccctt gaacagttgc aagcgtgttt cgcacacgat ggtgcagctc gcgaataagg
     4681 agcgcttgcc gctgctccgt gttgacatgg tccgtgacat cgttgccttc gataaaaatt
     4741 cccgtgacct cgcccttggc gtccataata ggctgggcga cgaaatcgat ataatgttcc
     4801 tccatctcac aatccgggga gtcctgcaga agaatgcgag tcttgcggcc aacatgaact
     4861 tccccggtct taaaaatatt ctctaaaatg cgggggtatt cttggccttc acactcaggc
     4921 agtccctcgc gcaggctctt cccgatgaga tcacggccac ccgcaagcat cgaaaaagcg
     4981 atattgacca attcaaacac cagtctaggt ccacgtaaaa tacacataaa acttggggcc
     5041 tgcatcacga gccggcgcag gtgctggacc gtcgctaaga gggtctggtt aagaaccagc
     5101 agatttccac tttgagggct aaattctccc atgcgcgccg ccgagggcag tccctcaaat
     5161 ccctttgagg acggcagctc ggaaatttcc tgcgctgtct tgagcacata ggcaatttgt
     5221 ccgtccttgt cacgaaccgg aatatgggcg cagctccaga gatgctcttc gtcacagcca
     5281 tctcgggcac aattgtagga gaccaaggga accacatcgg tgacaccctg gtcacgggca
     5341 cgttcgagtg attgttgcaa aatcccgcga ctttcttgcg ccgcggggaa agcggtaagg
     5401 atattgttcc caacgaccgc ttcccgggac gtctttgttg cctggagaaa agcccgattc
     5461 aaggcaatga tcgtgagatc ccggtccata actaaaagcg gcgtaggcag acaatcgaaa
     5521 acggcctcga aatccggctt tgttgggggc gccatataga actccaggaa atgaatgacc
     5581 ggtacagtga aaccgcgctc aaccggtgtg ttcgccctta gattgctttc ctcatgaaac
     5641 gaggttttcg gccagatgcc gtttagaaca gcgcgttatg tcggcgtccg gctttggtgc
     5701 ggatcaataa gatggacaac aggcctacat gacaagcctc aaaagaaaca cgccccagcg
     5761 ccgtgaacgg acctccaggt tcgcctttct ggaatgccta acgaataagc agtaccaatg
     5821 gttgcataga aatgccgctt gctaaggcgg tttttttcct cttcctagct cagccatgag
     5881 aaacgccgag acttagcccc accagcctaa cgaaagaaat gtcacgacaa atggccgagg
     5941 ctgaccgccc tctccaagca ggagatcatg ttttcctagt cgatggctcg tcgtttgtct
     6001 ttcgcgccta ttttcagtcg attcgacagg atgccaaata taactaccga tccgaccagc
     6061 tgccaactgg ggcggtaaga ctcttttgca ccaaactttt ccagttcctg cgcgagggcg
     6121 cggcgaagat caagccaacc catctcgcga tcatcttcga taaatcggaa aattcgttcc
     6181 gtagggagct ctacccgcca tataaagcca accgctcgga accgccggat gacctgatcc
     6241 cgcaatttcc gctgatgcgt gcggcggtgc gggcgtttgg actgacccct attgagcagg
     6301 accgcttcga ggcggacgat ttgatcgcca cctacgcctg cgaggcccgc gggcgcggcg
     6361 cggatgttct tatcgtttcg gccgacaagg atttgatgca gctcatcggc ccaggcgttg
     6421 caatgtatga tccagcctcg ggagaggctg gaagcaaggg tgcacgtgac gagcgcaaga
     6481 tcggtacgga agaagtgcgc gaatacttcg gcgtgccacc cgaaaaagtc gtcgatgttc
     6541 aggccctcgc tggcgattct accgataatg ttcccggcgc acgcggcata gggcttaaga
     6601 cagcagctca actcatcaac gaatatggcg atctcgaatc attgcttgct cgggcaaatg
     6661 aaatcaaaca gccaaagcgc cgcgaaaatc taactgatcc cgacagcgta acgcacattc
     6721 gcatatcaaa acagctggtc tcgctcgtcc gcgatgttcc gcttgaagtg ccgatcgatg
     6781 atcttgccct acatgcgccc gatggcaaga cgctcgtcgc ctttctcaag gcgatggagt
     6841 tttccatgat taccaaacgc gccgcagagg ctttcggcgt aacggcggat ctgattgagc
     6901 cagatcctga atttgtgggt gctgctggct ggcgcggcag cgatagggcg ggtccagcta
     6961 tcgccgccgg cgaagcggac aaatccccgt ccgcgaacat gagtcgcggg gcacccaaaa
     7021 gaaccgcgcg ttatggccag caaacccttg tcacgccgtc cgcaaccgga ccaaaccatc
     7081 tcgtagaagc acgcgccagc gcagctcgca aggaaaaact cgacaccaaa agctatgaaa
     7141 cgatttcgcg ttttgaagag ctcgacgcct atatcgctgc agccctcgag accggcgcga
     7201 tttcgattga tacggaaacc tcgaccctgc aaaccataca ggccgagctc attggcattt
     7261 ccctatgcct tgcgccgggg cgggcctgct acatcccatt gcttcaccgt gccgaaatca
     7321 gcgaactttt ctccagcagc gatcttcagc ctggccaact tcctgaaacg gaggttatcg
     7381 cgcgactcaa acctctgctc gaagacgcga gcgtcctcaa aatcgcgcaa aatatgaaat
     7441 tcaactggct gatttttaag cagcgcggcg ttaccgtacg gccggtcgaa gacacaatgc
     7501 tcttatccta tgtgctcgat gcaggccgca cggaccacgg aatggacgcg ctgagcgaga
     7561 accatctcgg tcatacgcca attaaaatct ccgacgtggc aggttccggg cgttccctca
     7621 ttggtttttc ccgggccgca attgaccggg ccaccgaata ttcggccgag tgcgccgata
     7681 tcaccctgcg actctggcga gtcttgaagc cgaggcttcc cgccgagcac atgaccaatg
     7741 tttatgagac attggagcgt ccaatgatcg aagttctg
//
LOCUS       DATMPU010000113         6100 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_2884669,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000113 DATMPU010000000
VERSION     DATMPU010000113.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 6100)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6100)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6100
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2884669"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(268..1407)
                     /gene="dnaN"
                     /locus_tag="VNY06_02655"
     CDS             complement(268..1407)
                     /gene="dnaN"
                     /locus_tag="VNY06_02655"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012319207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HWX13764.1"
                     /translation="MKVTLERAALLKSLGHVHRVVERRNTIPILSNVLLHGENQSLLL
                     KATDLDLEVTERLPADVSAAGVTTVPAHVLYDIVRKLPDGAQVSLESHGESGQLQLRS
                     GRSRFNLQSLPDADFPDLTTGDLSHSFKLASGDLKRLIDKTQFAISNEETRYYLNGVY
                     LHTMDVEGHTMLRAVATDGHRLARVEVPAPKGALAMPGVIVPRKAVAEIQKLVENLDQ
                     DVRIEISPSKARFTFGDPSVHLGQVVLTSKLIDGTFPDYMRVIPSGNDKRLTVDREPF
                     QVAVDRVATISSERGRAVKLTLADAKLILSVTNPDLGSAVEELDVDYDSSPIDIGFNA
                     RYLLDITEQLDSDTVLFKLSDPGSPTLLQDRDGAAALYVLMPMRV"
     gene            1818..3791
                     /locus_tag="VNY06_02660"
     CDS             1818..3791
                     /locus_tag="VNY06_02660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018264527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HWX13765.1"
                     /translation="MLYWGAIATVWVAVAGTGVIAFEATKLPPIDALAVPKRPPNIAI
                     LDENGVLLANRGDTGGAAVKIGDLPLYLPKAFIAIEDRRFYSHFGVDPFGVLRAVYHN
                     ISNGGTVHGGSTLTQQLAKNLFLTQERTVSRKIQEAILALWLEHNYAKNQVLELYLNR
                     VYFGSGAYGVEAAAQKYFGHGARDVTLQEAAILAGLMKAPARLAPNRNPAGAQERAQQ
                     VILAMAGEGYISNAMARLALAHPAQVLRAQNKNSANYAADFVMDILDDTVGAINGDIT
                     VSTTLNASLQLGAERALREELDRYGTAFGVEQGALVAMDPNGAIKALVGGRNYNESEF
                     NRAIAAKRQPGSAFKPFVYLAALESGLTPDTVRQDGPINVKGWQPENYSRAYYGPVTL
                     MQALSLSLNTVAVRLGLEVGPKAVETVAHRLGIASELAVNPSLALGTSEVTPLELVSA
                     YAPFANGGIGVQPLIIWKVKTAAGKLLYQRRGASNGRVIDPNYVGMMNSMLQETLLTG
                     TARKAELPGWQAAGKTGTSQEFRDAWFVGYTSHLIAGVWLGNDDGSPTKKASGGTLPV
                     AIWSRFMKAAHQGVPPQPLPGGWWRANPAPFANVPAADSGPALIPSSDWPTGARGTGV
                     PLPPANVPNPSSAARSRAARPSGRSFFDSLFGG"
     gene            complement(3806..4573)
                     /locus_tag="VNY06_02665"
     CDS             complement(3806..4573)
                     /locus_tag="VNY06_02665"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020174510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWX13766.1"
                     /translation="MTEPLVSFQSAGKIYETGTVALAGLDLEVRAGEFLTLLGPSGCG
                     KSTVLRLTAGLEELTSGSLTRGFELDQASIGFVFQDPTLMPWANVFDNVYLPLRLRGT
                     KRHDAAPTIEKALALVGLSSFSRAFPRELSGGMRMRCSIARALMLRPLLLLMDEPFAA
                     LDEITRFKLNDDLLRLQCELGTTIVFVTHSVFESVFLSSSIVVMAARPGHVVAKIAID
                     SPSPRSMEFRTSSLYARYCAEASRALHRGMEGSGKDE"
     gene            complement(4606..5595)
                     /locus_tag="VNY06_02670"
     CDS             complement(4606..5595)
                     /locus_tag="VNY06_02670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018316418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWX13767.1"
                     /translation="MCLLPLLSALALAASPAAALDKVTFATNWLAEAEHGGFYQAKAD
                     RTYEKYGLDVTILQGGPNANNRLSLAAGKIEFDLGANLIQAFDAVSQNIPILAVAALF
                     QKDPFVLLSHPGVGLDHIEDLPKATAFIGKDGFVSVYQWLKLVYGFADEKVRPYTFNS
                     APFLADKNSIEQGYATSEPFEVERLGKFKPNVFLIADYGYDSYSTTIETTRELIEKNP
                     DLVQRFVEASIIGWYNYIYGDNRAANALIKRDNPDISDDQLAYSVTKMKEYGIVDSGD
                     SLTLGIGAMRDERIASFFAKMVRAGLFKPNLDYKRAYSLQFVNKGVGLNLRLH"
     gene            complement(5905..>6100)
                     /locus_tag="VNY06_02675"
     CDS             complement(5905..>6100)
                     /locus_tag="VNY06_02675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020175127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX13768.1"
                     /translation="TGMPYALDQTPKAYLTQMALYHAVLAPLWPEKSVKTLLIWTAGP
                     RAIWLPGEMLDAALAALAAP"
BASE COUNT         1377 a         1688 c         1736 g         1299 t
ORIGIN      
        1 tgtcgcgtaa ccggcgcatg tagagcaagg tgactttatc ttcgaatcgc cgtcttgctc
       61 tcattcatcg tttcggcgcg tgtccggacg caaaacctgt tcccattatt ggctcgacaa
      121 cctcgagacc ttgcctcgtc cttatgagcg aacgacttca acacacgcat taacaaaaaa
      181 agttgcgcgc acagcctggg cgagcaaagc tgcttaaagg aggtcgcttt tcgtcgttag
      241 ccctgccgta aaattgtttt gaactgctca tacccgcatc ggcatcaaaa catagagcgc
      301 agcggcgcca tcgcgatctt gcagcagcgt gggagaaccg ggatcggaaa gcttgaataa
      361 gacggtgtcg ctgtcgagct gctcggtaat gtcgagcaga tagcgggcat tgaatccaat
      421 gtcgattggc gacgagtcat agtcgacgtc aagttcttcg accgctgagc caaggtcggg
      481 attggtgacg gaaagaatga gtttcgcgtc agccagggtc agcttaacgg cccggccccg
      541 ctcggacgaa atcgtagcaa cgcggtcgac ggccacctgg aatggctcgc ggtcgacagt
      601 caggcgcttg tcattgccag acgggatgac gcgcatatag tccggaaacg tgccgtcgat
      661 caatttggag gtcagtacga cttgtcccaa atgcaccgaa ggatcgccga acgtaaagcg
      721 ggcctttgac ggtgagattt cgatgcgaac gtcttggtca aggttctcta cgagtttctg
      781 aatttcggcc accgccttgc gcggcacgat aacgcctggc atcgccagag ctccttttgg
      841 ggctggaact tccacccgcg cgagcctgtg accgtcggtc gcgacggcac gcagcatcgt
      901 gtgaccttcg acatccatcg tgtgcagata aacgccgtta agatagtagc gtgtttcttc
      961 attggaaatt gcaaattgag tcttgtcgat caagcgcttc agatcgcccg aagcaagttt
     1021 gaagctatgg ctcaggtctc ccgtggtgag gtctgggaaa tcggcatcgg gcaaagactg
     1081 tagattgaac cgcgaacgtc cggaacgcag ctgcaattgg ccagactctc catgcgactc
     1141 gagtgatact tgagcgccat cagggagctt gcggacgatg tcatagagaa cgtgagcggg
     1201 caccgttgtg acgccggctg cgctgacgtc tgccggcagg cgctcggtaa cctcaaggtc
     1261 gagatcggtc gctttgagga gcagcgattg gttttccccg tgcaacaaaa cattcgacag
     1321 aatgggaata gtattgcgtc gctcgacgac gcgatgcaca tggccgagag attttaacaa
     1381 ggccgctcgt tcgagagtga ctttcatagt gttatccaat cggggcattc gttcgatccg
     1441 tcacatgtgc cacgcgccgg agaaaggggg ctgactttgg cgcggctttg gccaaaccgc
     1501 aagcggctgc gggagcacca ccctacgctt ttccccgggc gatcaaaggg caagcggcgc
     1561 gccgaccctt tacctgccct taatccgctc aatttaaaat aaaaaaagcg ccatctcccc
     1621 caggaaatgc ggccgctgac gcatcgtcga tgaattggca acgcggtggc acggttagaa
     1681 tggcatagcg taaatcgccc cggcgctcgg agcttgccta ttgccaacct tcatcaacca
     1741 ttaggcccga aaccgtgcag cgctacagcg taagatcacg gagccaaacc aagaagcgtc
     1801 gctcgcttct tgcccaattg ctctattggg gtgcgatcgc aacggtctgg gtcgcagtgg
     1861 ccggcacggg cgtcatcgcc ttcgaagcaa caaagctgcc gccgatcgac gctctggcgg
     1921 ttccgaaacg gccgccgaac attgcgattc tcgacgaaaa cggagtcctg cttgcaaacc
     1981 ggggtgacac cgggggggcc gcggtcaaaa tcggtgactt gcccctatat ctgccaaagg
     2041 ccttcattgc aatcgaggat cgccgctttt attcgcattt tggtgtcgat ccctttgggg
     2101 ttttgcgcgc cgtctaccat aatatttcaa acggcggcac agtgcacggc ggatcgacgc
     2161 tgacgcagca gctcgccaag aacctctttc tcacccagga acgaaccgtt tcgcgcaaga
     2221 tccaagaagc aatcctcgca ctctggctcg agcacaatta tgcgaagaat caagtcctcg
     2281 aactatattt gaaccgcgtt tatttcggct caggcgccta tggcgtcgaa gctgctgcgc
     2341 aaaaatattt tggccatggc gcgcgcgatg tgacacttca agaagcggca attcttgcgg
     2401 gcctcatgaa agctccggcg aggctcgcac ccaatcgcaa tccagcgggc gctcaagagc
     2461 gcgcccaaca agtcatttta gcaatggctg gggaaggcta catcagcaat gctatggcaa
     2521 ggctcgctct tgcccatccc gcgcaagtcc tgcgcgctca gaacaaaaac tcggccaatt
     2581 atgccgctga tttcgtgatg gacattctcg acgacacggt cggtgcgatc aatggggaca
     2641 ttactgtctc gacgactctc aatgcatcat tgcagctcgg ggcggaaagg gcgctgaggg
     2701 aagagcttga tcgctatggc acggcttttg gggtcgagca gggcgcgctc gttgcaatgg
     2761 atcccaatgg tgcgatcaag gcgctggtcg gcgggcgcaa ctataacgag agcgagttca
     2821 atcgtgcgat cgcagccaag cgccagccgg gctctgcatt caagcctttt gtttatcttg
     2881 cggcgctcga aagcggcctc accccagaca ccgtgcgcca agacgggccg atcaatgtga
     2941 aaggctggca gccggaaaat tactctcgtg cctattacgg cccagtcacg ctcatgcagg
     3001 cgttatctct ttcattgaat acagtggcgg ttcgcctcgg tcttgaagtc gggccgaagg
     3061 ccgtcgagac cgtcgcgcac cggcttggca ttgcgtccga acttgccgtc aatccctcgc
     3121 tcgcgcttgg gacatcggag gtcactccgc tcgaactcgt cagcgcctat gctcccttcg
     3181 ccaatggcgg aatcggcgtt caaccactga tcatttggaa ggtgaagacc gcggcgggta
     3241 agctgcttta tcaacgcagg ggcgcaagca acggaagggt tatcgatccg aactatgtgg
     3301 ggatgatgaa ttccatgctg caggaaaccc tgctgaccgg gaccgcgcga aaagccgaat
     3361 tgccgggctg gcaggcagcg ggcaagaccg gtacgagcca ggagtttcgt gatgcctggt
     3421 tcgtcggcta cacgagccat ctcatcgccg gcgtatggct tggcaatgac gatggttcgc
     3481 caaccaaaaa agcctctggc ggcactttgc cggtcgcgat ctggtcgcgc ttcatgaaag
     3541 ccgctcatca aggcgtgccg ccgcaaccgc ttccaggcgg atggtggcgc gccaatccag
     3601 cgccgtttgc gaatgtgccg gcggcggaca gcggccccgc tttaatccca tcatctgatt
     3661 ggccgacagg ggcgaggggg accggcgtcc cactgcctcc ggccaacgtc cctaacccca
     3721 gttccgccgc gcgctcccgc gcggcgaggc caagtggccg gagttttttt gattctctct
     3781 ttggcggcta aagccgcatt tctctttact catctttgcc ggagccctcc atccctcgat
     3841 gcaaagcgcg cgaagcttcc gcgcaatagc gagcatagag ggaactcgtg cgaaattcca
     3901 tgctgcgcgg tgaaggagag tcgattgcta tttttgcgac gacatgaccg ggccgtgccg
     3961 ccatcactac gatcgagctt gacagaaaaa cgctttcgaa aaccgaatgg gtcacgaaga
     4021 cgattgttgt tccaagctcg cattgaaggc gcagaaggtc gtcgttaagc ttgaaccttg
     4081 tgatctcgtc gagcgcggca aaaggctcgt ccatcaatag aagcaaagga cgcagcatga
     4141 gcgcgcgggc gatagagcaa cgcatgcgca tcccgcccga aagctcgcgc ggaaaggctc
     4201 ggctaaagga agaaaggccg acaagcgcca aggccttttc gatcgtcggc gcagcatcgt
     4261 gccttttcgt gccgcgtaac cgtagcggca aataaacatt atcgaagacg tttgcccacg
     4321 gcatcaaggt aggatcctgg aacacaaagc cgatgcttgc ttgatcaagc tcgaagcctc
     4381 gtgtgagcga gcctgacgtc aattcttcaa gacccgcagt gagccgcaga acggtcgact
     4441 ttccgcagcc cgacggtcca agcagagtca aaaactctcc tgcccgcacc tcgaggtcga
     4501 ggccagcgag cgcaaccgtt ccggtctcat agatttttcc agcgctttga aaagagacga
     4561 gcggctctgt catcacggaa atagtagagc ctcgcttttg cctgttcaat gcagccgcag
     4621 attgagaccc acacccttgt tgacgaactg caagctatag gcgcgcttat aatcgaggtt
     4681 gggcttgaaa agtcccgccc gcaccatttt ggcaaagaag ctcgcaattc tttcgtctcg
     4741 catggcgcca ataccaagcg taagagagtc gcccgaatcg acaatgccat attccttcat
     4801 cttggtcacg gaataggcaa gttgatcgtc gctgatatcg ggattgtcgc gcttaatgag
     4861 agcattagcg gcgcgattgt cgccatagat ataattatac cagccgatga tcgaggcttc
     4921 gacaaagcgc tgtacgaggt ctgggttttt ctcgatcagc tctcgcgttg tttcgattgt
     4981 ggtcgaatag gaatcatagc cgtaatcggc gatcaaaaac acatttggtt tgaatttccc
     5041 cagtcgctcg acctcaaaag gttccgaggt cgcatagccc tgctcgattg aatttttgtc
     5101 ggcaaggaag ggcgccgaat taaaagtata gggccttacc ttttcgtccg caaaaccgta
     5161 gacgagcttc agccattgat agaccgagac aaagccatcc ttaccgataa aggccgtggc
     5221 cttcggcaaa tcctcaatat ggtcgagacc aacgcctgga tgcgacagca aaacaaaagg
     5281 gtctttttga aaaagcgctg cgaccgcgag gatgggtatg ttttggctca ccgcgtcaaa
     5341 ggcctgaatc aaattggcgc cgaggtcgaa ttcgattttt cccgccgcga gcgacagcct
     5401 gttgttggca ttggggccgc cttgcaggat ggtgacgtcg agcccgtatt tttcataagt
     5461 tcggtcggcc ttggcctgat agaagccgcc atgctcggct tcggcgagcc agttggtggc
     5521 gaatgtcacc ttgtcgagcg cggcggccgg agaagccgca agggcaagcg ccgaaagcag
     5581 ggggagcaag caaattcgtt tcaaggcgaa tcctcgatcg ttttgtagaa cccgtgcgcg
     5641 cgatagggcg tcgttgtccg attttgcccc ggaatcggac aaaaatgttt caggtgaaac
     5701 attttagtac gataattggc gaaaacctta caagcctcat acagcgcgtg gccttgattt
     5761 gcgcaggata gggcgaaaaa tcggtgcttt tggcagtttt gaaatatacc gtccgttctg
     5821 gcacattttt taagatctcg aaaagcgggc gatcctttag gtggccgctt gacaattaaa
     5881 actcgatcgc cgatggcccg gcgatcacgg tgccgcaaga gcggctaaag ccgcatcaag
     5941 catctcccca ggaagccaaa tggcgcgagg ccccgccgtc cagatgagca gcgtcttaac
     6001 gcttttttct ggccagagcg gcgctaaaac tgcgtgataa agggccattt gcgtgagata
     6061 agcttttgga gtctgatcga gcgcgtaggg cattccagtc
//
LOCUS       DATMPU010000114         3416 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_4110722,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000114 DATMPU010000000
VERSION     DATMPU010000114.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 3416)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3416)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3416
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4110722"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(246..1586)
                     /locus_tag="VNY06_02680"
     CDS             complement(246..1586)
                     /locus_tag="VNY06_02680"
                     /inference="COORDINATES: protein motif:HMM:NF016311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase N-terminal domain-containing
                     protein"
                     /protein_id="HWX13769.1"
                     /translation="MLASALSPIEISVQALKPSLLLSAYRLASAGLGAAGPLYLYWRG
                     KIGRDDFLRRHERLGRSYLARPGGRLALLHMASSADTLGAKALVEKLGQLGFTVLLSI
                     RDPEFGTFQAPRFPPSLHQLSPLDTPQSVTRFLDHWRPDIVLLSGPEIRPILIFEANR
                     REVPLALVNARVSARSFLIWRGFLGFSASLLSRLDIALAQTNADAERLRALGARSVRV
                     TGSLKYDVAPAPADQFALARLVARIGTRPAWVADGIALSEKEIVITAHRHLARQFSNI
                     LTVIVPDNPKHAFEIAQTAAQMGFIAGIRGADRKTAPFPDIYIAHTADEAGLFYRGAG
                     VVFVGKSLSRGGGKNPVQAAQLGCAILHGPEIDEFEDLFAALDNSGGGMLVFDADTLA
                     KQLALLFFDKAELRAMARAAAETAEFFGGASTLTIEAIAPYIAQVMVAPKAIGG"
     gene            complement(1558..1833)
                     /locus_tag="VNY06_02685"
     CDS             complement(1558..1833)
                     /locus_tag="VNY06_02685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016552914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4170 domain-containing protein"
                     /protein_id="HWX13770.1"
                     /translation="MTTQDKQLLHFVFGGELTDLSGTEFRDLSNIDIVGIYPDYASAL
                     QAWKAKAQATVDNAHMRYFIAHLQKGQRVSAASTVPIGNARKCAVPN"
     gene            complement(2092..3201)
                     /locus_tag="VNY06_02690"
     CDS             complement(2092..3201)
                     /locus_tag="VNY06_02690"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HWX13771.1"
                     /translation="MTNADGLTINGRFLTQSMTGVQRYAREIIIELDQLLKSCPLREG
                     MHTKLIVPAGTKTDLALRAISIKETRLGDGPLWTQIVLPFVKQGVLLSLGNIGPVLAV
                     KQVICIHDLNTFLVPESYSRAFRLYYRAALTPLARNAARVVTVSRFSADMLHKFGLRR
                     REEIVVIPNGHEHVKRWRPERSPYAAKGERQRPFVFVLGSRAVHKNVEMLFSIAQELD
                     ASGLDIWVAGGSGSYFSAVDPRPAPPNVRMLGFVTDDDLAALYGHALCFAFPSLTEGF
                     GLPVLEAMALGCPVVASNAASLPEVCGDAGLLADPTSPGDWLNQIGRLEADPGLVRDL
                     RAKGLGQAQHFSWKASAKAYLDLMAPFLGPAARTN"
BASE COUNT          782 a          999 c          983 g          652 t
ORIGIN      
        1 atctctatgg gtcggccgat tggcaacata gcggacattg cctttcatca agcggctgag
       61 cgcggcatcc gcgccgatgg cgtttggcgc cggctgacca gcgcacgaga gacacatgtt
      121 tggcgatcct atttttcaca gcatttttaa gcggtaatct catcgcgacc gcgtgacaag
      181 agtgaagccg gacttgatag caggcccgac cgcagcatcg catttcagct tgcttgcctt
      241 gacccttacc cgccaatagc cttcggcgcg accatcactt gcgcgatgta aggcgcaatg
      301 gcctcgatgg tgagcgtaga ggcgccgcca aaaaactctg ccgtctcggc cgccgcgcgc
      361 gccatcgcgc gcaattccgc tttatcgaag aagagaagcg cgagttgctt ggcaagcgta
      421 tcggcgtcaa acaccagcat gccgccgcca gaattgtcga gagcagcaaa aagatcttca
      481 aactcgtcaa tttctggccc atggagaatt gcgcagccga gctgcgccgc ctggactgga
      541 ttcttgcccc cgccacggct taaggacttg cccacaaata cgacgccagc gccgcgataa
      601 aacaatcctg cctcgtcagc cgtgtgggca atataaatgt ccgggaaagg ggcggttttg
      661 cgatctgcgc cacgaatccc ggctataaaa cccatttggg cggcagtttg ggcaatttca
      721 aaagcatgtt tggggttatc cggtacaatc accgtgagaa tatttgaaaa ctgccgcgcc
      781 aagtggcgat gggccgtaat cacgatctcc ttctcggaga gagcaattcc gtcagcaacc
      841 caggcaggcc tcgtgccaat acgtgcaacc agtcgcgcca aggcaaattg atcggcgggc
      901 gcgggcgcga catcatattt gagactgccc gtcacccgca cggatcttgc gccgagcgcc
      961 cgcaagcgtt cggcatcggc gtttgtttgc gccaaagcaa tatccaacct gcttagcagg
     1021 gaggctgaaa agcccaaaaa tccgcgccag attaaaaaag agcgggcaga aacgcgtgca
     1081 ttcacgaggg ctagaggaac ctcccggcgg ttcgcttcaa aaataaggat cggccggatt
     1141 tcgggcccgg agagcaggac aatgtctggc ctccaatgat caagaaaccg cgtgacggat
     1201 tgcggcgtat cgagaggcga aagctgatgc aacgacggcg gaaaccgcgg cgcctgaaac
     1261 gtgccgaact ccggatcgcg gatcgacaaa agcacggtaa agccaagctg cccgagcttt
     1321 tcgacaagcg ccttggcgcc aagtgtatcc gcgctgcttg ccatgtgcag caaggccagc
     1381 cggccgccgg gccttgcgag ataggaccgc ccgagccgct catgccgccg caaaaaatcg
     1441 tcgcgcccga ttttcccgcg ccagtaaaga tatagtggcc ctgccgctcc gagccccgcg
     1501 cttgctagcc ggtaagcaga aagaagcagc gaaggtttta gggcttgcac ggaaatctca
     1561 attggggaca gcgcacttgc gagcattacc gataggaacg gtagacgcgg cgctcacgcg
     1621 ttgtcctttc tggagatgcg ctatgaaata acgcatatgg gcattgtcca cggtggcctg
     1681 cgccttggct ttccaagcct gcagcgcaga cgcatagtcg ggataaatgc cgacgatgtc
     1741 gatatttgat aaatcacgaa attccgtgcc gctcaaatcc gtgagttcgc caccaaatac
     1801 gaaatggaga agctgcttgt cttgcgttgt catgagcttc ccgttcgaag ttctgacgga
     1861 agatgccttt ccctccatct aaagctttcg ccatagtctt ggaagaggcg accttgccct
     1921 tctgttgagt acgcaaggcg cgggccacac ggttctcgcg cccataagac cggggtgaaa
     1981 caaggaccag gccgcaagtt cgctctccgg gaaatttgga gacccagcaa gttttcagcc
     2041 agccgcgttc cgaattttgc agtatccgtc tggcatcctt aaggggctca ttcaattggt
     2101 ccgcgcggca ggacccagga atggcgccat gaggtccaaa taagctttcg cgcttgcttt
     2161 ccaggaaaaa tgctgagctt gccccaggcc cttggcgcgc agatcgcgca ccaggccggg
     2221 atcggcctca aggcggccga tctggttaag ccagtcgccc ggggatgtcg gatcggcgag
     2281 aagcccggcg tcgccgcaga cctctggcag gctcgccgca ttggaggcaa cgacagggca
     2341 acccaaggcc atcgcctcaa ggacaggcag accgaatccc tctgtcaacg aaggaaacgc
     2401 gaagcaaagc gcgtgcccgt aaagcgccgc gagatcgtcg tcggtgacaa acccgagcat
     2461 gcgcacattt ggcggtgcgg ggcgtgggtc cacggccgaa aaatagctgc ccgaaccgcc
     2521 ggcaacccaa atatccaacc ccgaggcgtc gagttcttgg gcgatcgaaa acagcatttc
     2581 aacattcttg tggacggccc ggctgccaag cacaaagacg aaaggccgct gtctttcccc
     2641 ctttgcggca taaggcgagc gctctgggcg ccagcgcttg acatgttcat gaccattggg
     2701 aatgaccacg atttcctcgc gccgccggag accgaattta tggagcatgt ccgcggaaaa
     2761 gcgcgagacc gtgacgacgc gcgcagcatt ccgcgcaaga ggcgtaagcg cagcccggta
     2821 atacaagcgg aacgcgcggc tatagctttc cggcacgaga aatgtgttca gatcgtggat
     2881 gcagatgacc tgctttacgg ccagtaccgg cccaatgttg ccgaggctta gcagcacgcc
     2941 ttgtttcaca aagggcaaga caatttgagt ccagagcgga ccgtctccga gcctggtctc
     3001 tttgattgaa atcgcgcgga gagccaaatc ggtctttgtg cccgctggaa cgatgagttt
     3061 ggtgtgcatg ccttctcgca gcgggcatga ttttagaagc tgatcgagct cgatgataat
     3121 ttcgcgcgcg tatctctgca cgccggtcat gctctgcgtg agaaaacggc cgttgatggt
     3181 cagcccatcc gcatttgtca ccaacgggcc tcattcaaga tcaaaaagac tggcgcgaca
     3241 aatttttacg gatcgatttc ctgcacaaac gtaatcccgc agcgctccga gccggactgt
     3301 ctgcacgcgg catgcgtgcc ggctgacaca agccgcggct caggcccaat gcgccgagtc
     3361 caaaatcctc tcccatcgat ttcagtcatg cgggacgagg aggtgggacc caaccg
//
LOCUS       DATMPU010000115         3001 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_1635458,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000115 DATMPU010000000
VERSION     DATMPU010000115.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 3001)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3001)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3001
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1635458"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(201..776)
                     /locus_tag="VNY06_02695"
     CDS             complement(201..776)
                     /locus_tag="VNY06_02695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX13772.1"
                     /translation="MHRGEHEPILSHELFAAVQAEIAANAVARQIRLSGSAAILAGRL
                     FDDRGNRMSPTHANKRGVRYRYYVSHAILQNRKTEAGSIARVPAPDVENLVCDGVRRH
                     LAASGEVEPPTTLADRDLIERHVERVIIKPEALEVCLIPPCEASDQAEDPIVDEQASC
                     RPQMATIGLAWTAPNFSVVKGIIHAPSAKRR"
     gene            1507..>3001
                     /locus_tag="VNY06_02700"
     CDS             1507..>3001
                     /locus_tag="VNY06_02700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015950511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching N-terminal
                     domain-containing protein"
                     /protein_id="HWX13773.1"
                     /translation="MDAAIELSEHYIEVQGSLVQRRLRSLKHGDAFAVFDDYGDIGVI
                     GGGPEGLYFNDTRFLSWYGLRFEGKRPLLLGSVVQDDNAALSVSLANPDIHSDGLISL
                     PRDTIAIQRTKFLWQAVCYERIGFHNYADSIRHFCIDIGFGADFRDLFEVRGTSRERR
                     GTCTSVVTEEVVGFYYEGLDNLKRKTELRFWPLPSRVETNRATFEIELAPNARKSLFV
                     SVLCEDRNKPRCDSFGHAFRARRRAIRYTATGIATIESSNELFNEVCRRATADLYMLM
                     TRTSYGLYPYAGIPWYSTVFGRDGIITAMLLLWLDPSVAKGVLGFLAETQAKTFDPLS
                     EAQPGKILHETRNGEMARLGEVPFRHYYGSVDATPLFVMLAGMYFDRTGDLGTIAAIW
                     PNIKAAVRWIDTFGDADGDGFVEYARQTKSGLVNQGWKDSYESIFHADGALAIGPIAL
                     CEVQGYVFAAKKQAAKLAHRMGEVDLETKLRGEAQILRKNFEAAFWCQ"
BASE COUNT          633 a          815 c          886 g          667 t
ORIGIN      
        1 ccctataacg cctaacttct atttaagttc ccgtcgggcg acaggaaggc gaggggagcg
       61 agcaggcgga tgtgccgttc gccttggggc cccaagctct gcgacctcgg cgaaggtggc
      121 gacgcggccg agccggatgt catcgatcca tctccgagct ttggcaattg cggcaagcaa
      181 agcatcacgg ctttcgggcc tcatcgccgt ttagccgagg gcgcatgaat gatgcccttc
      241 accaccgaga agtttggcgc agtccatgcg agcccgatcg tggccatctg cggacggcag
      301 gaagcctgct catcgacgat ggggtcttcg gcttgatccg acgcctcgca cggaggaatg
      361 aggcagactt ccaatgcctc gggtttgata atcacgcgct cgacatggcg ttcgatcagg
      421 tcgcgatccg caagggtggt cggcggctct acttcccccg aagctgctag atgcctgcgc
      481 acgccgtcac acacgaggtt ctcaacgtcg ggagccggca cgcgggcaat gctgcccgct
      541 tcggttttgc gattttgcaa aatcgcatgc gagacatagt accggtagcg cacgccgcgc
      601 ttgttcgcat gcgtcgggct catccggttg ccgcgatcgt caaagagacg gccggcgagt
      661 atagccgccg agccactgag ccggatttgc cgggcaacgg cgttggcggc aatttcggcc
      721 tgcacggccg cgaacagctc gtgactgagg atcggctcgt gctcgccgcg atgcactttg
      781 ccccgggccc gaggaggaac aggtactggc ggccaaacct ggcagccgga ttcctcaagg
      841 cggccgcttt acaacaagtt tgagatcgcg aaacaaattc tctcgaacat agcgtgaccg
      901 cgcgaaatag cacgatcgct cgcatttgtg cgccgcaatg cactgtggtg cgcgaatggg
      961 tcatgtttcg gaaaaagata tgagccaaga gcagaagaat aggtcagtac aattcttgcc
     1021 gcattgctgg agtttttatt gagacttgag attggcattt aggtgaagat catgacacac
     1081 ttcccgcttg atccgccacg tatcaacaaa gaggacagaa aacaacgttc ctcgtactgc
     1141 catgagtgtc ttgagcggtt cacggcaagc gatgtgcgct gcagctatgg caaccgattc
     1201 tttcacaagg agtgctacaa gcgctggcag gaaaagagga tatttcaatg tgttagttcg
     1261 ttaaacagcg gccaatacga tcgctgcggg gcgtaacaaa ctcccattct ttgatctcca
     1321 aaagttagat ccatatagat cggcggtcat gccgccagtc tatgtctagt gttcaacttt
     1381 gggcgcggcc tggcggaaca aggccgatag gcatggccgc tacccttcac atctgaaagc
     1441 gtactttcag ggagaacgga catcgttggc agcacgcttg agacggtgcg tcgcatagaa
     1501 agcgtcatgg acgcggcgat agagctgtcc gagcattata tcgaggtcca ggggtctctc
     1561 gtccagcggc ggctccgtag cctgaaacat ggcgatgctt ttgcggtctt tgacgattac
     1621 ggcgacattg gcgtcatcgg cggcggaccc gaaggacttt atttcaacga cactcgcttt
     1681 ttgtcttggt atggactccg tttcgagggc aaacggccct tgttgttggg ctcggtcgtt
     1741 caggacgaca atgccgcgct ctcggttagt cttgccaatc ccgatatcca ttcagacgga
     1801 ctcatttcgc tgccgcgcga cacgattgcg atccagcgca ccaaattcct ttggcaggcg
     1861 gtctgctatg agaggattgg gtttcacaat tacgccgata gcattcggca tttttgcatc
     1921 gacattggat ttggcgccga ttttcgagat ctgtttgaag ttcgcggaac ctcacgcgaa
     1981 cggcgcggca cctgcacatc ggtagtaacc gaggaagttg tcggatttta ttacgagggg
     2041 ctcgacaatt taaagcgcaa gaccgaatta cggttctggc cgctgccctc tcgtgttgag
     2101 acaaatcgcg cgacgttcga aatagagctt gcgcccaatg cccgcaagtc gctcttcgtg
     2161 tcggttcttt gcgaggatag gaacaagccg cggtgcgatt ccttcggaca tgctttccgt
     2221 gcgagacggc gcgccatccg gtacacggcg accggcatcg cgactatcga aagttcgaac
     2281 gaactcttca atgaagtctg ccggcgggcc actgccgacc tttacatgtt gatgacgcgc
     2341 acgagctatg ggctttatcc ctacgcggga atcccatggt acagcacggt cttcggccgt
     2401 gacgggatta tcacggccat gttgcttttg tggctcgacc cgtccgtcgc caaaggcgtt
     2461 ctcggcttcc tcgccgagac tcaggcaaaa accttcgatc ctctttccga ggcacaacct
     2521 ggcaagattc tgcatgaaac ccggaacggg gaaatggcgc gccttggtga agtgccattc
     2581 agacactatt acgggtctgt cgatgccacg ccactctttg tcatgctggc cggtatgtat
     2641 ttcgaccgga ctggggacct cggaacgatc gccgcgatct ggccaaatat taaggcagct
     2701 gtgcgatgga tcgacacgtt tggcgatgcc gatggagacg gctttgttga atatgcgcgg
     2761 caaaccaagt ctggccttgt caatcaaggc tggaaggact cttatgagtc aatctttcac
     2821 gccgatggcg cattggcgat cgggcctatc gcgctttgcg aagtgcaggg ctatgtgttt
     2881 gccgcaaaga agcaggcggc gaaactcgcg catcgcatgg gcgaggttga tctggagacc
     2941 aagctgcgtg gcgaggcgca aattctgcgg aagaatttcg aggcagcttt ttggtgccag
     3001 g
//
LOCUS       DATMPU010000116         2765 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_2564742,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000116 DATMPU010000000
VERSION     DATMPU010000116.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 2765)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2765)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2765
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2564742"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(376..1926)
                     /locus_tag="VNY06_02705"
     CDS             complement(376..1926)
                     /locus_tag="VNY06_02705"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012321448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Do family serine endopeptidase"
                     /protein_id="HWX13774.1"
                     /translation="MGVLPVWRGIENAAGDAQRRTSTSAAFLVGCALALGIAGSVTPF
                     STPAVAKGPESLADLADGVSDSVVNISATQTIDDKHAGNAPELGPGTPFDDLFEEFFR
                     RHQQGGGERQDQKRHERKSNSLGSGFVIDASGIVITNYHVIADANEVTVILSDGQKLK
                     AEIVGKDEKVDVAVLRVKPEKPLKAAKFGDSEKMRVGDWVVAVGNPFGLGGTLTAGVI
                     SARHRNIDSGPYDNYFQTDAAINKGNSGGPLFNMAGEVIGVNTAILSPSGGSIGIGFA
                     TPAATVIPVIEQLQKYGETRRGWLGVRIQNIDDTIAESLNLGKLRGALVAGTDDRGPA
                     KAAGIKAGDVIIKFDGTEIKESRDLPKIVAAAPVGKDVEIVVMRQGKPIAKMIKLGRL
                     EENEKQASLGGTQGESKMKAPPGPVEKALGMEFSSLSEDLRQRYAIKSAVAAGVVITG
                     VDPDSSAAEKHLQPGDVLMEINQEPVKEPSDIAKKLEALKGSGKKSALLLVANGQGEV
                     RFVALGLP"
     gene            complement(2034..>2765)
                     /locus_tag="VNY06_02710"
     CDS             complement(2034..>2765)
                     /locus_tag="VNY06_02710"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HWX13775.1"
                     /translation="RSKLAGLAAATLILVLAVYGTALLANIPMAALAGTLMFVAIKLV
                     RPREILAVSRSAFGEFLLIFTTFAAIILLPVGTGVAIGIVLSLLHGMWSVTRPQIVAF
                     ERVPGTSIWWPPGRSTKGGTIDGVLVIAFQAPLSFLNAYAFQEGVQRVIRECPTPPSL
                     IVLEASSIIDIDFTAAQILLSFIKDCAQQKISVAVARLESLRAQDGFERFGITDALPR
                     THLFHSVDEAIRALVKKSNEFGTNS"
BASE COUNT          581 a          820 c          761 g          603 t
ORIGIN      
        1 cttcgaggcg tctgaccacg acgcggtcgt gcaaaggacg aaagctcatg tttctaggtt
       61 ccctactatg ccgctggcgg tgcgccgggg ttatacccag aacgccttgc caagcttgcg
      121 gctaaattga aacgacagtc cgttagcact cacaaacaga gagtgcgaac agcacgcccg
      181 gtaactaggg atcacgttcc ggtgtgtcaa gcggttaatt ttgaacttcg ctatctctgc
      241 ttcttgcgct ctccgagata gtgcaggggc aaattcagac tacccaaact ggatgaaaac
      301 gcccagccgc ggcgaaattt gcccccgccc tgcaagcctc cagaagtcgc cgtcccgccg
      361 ccagatgtct gctcgttaag gcaggccgag cgccacaaag cggacttcac cctgaccatt
      421 ggcgacaagc agcaacgcag atttcttgcc gctacccttc aatgcctcaa gtttcttggc
      481 gatgtcggac ggctctttga caggctcctg attgatttcc atcagaacgt cgcccggttg
      541 gagatgcttt tcggcggcgc tcgagtcggg atccacaccg gttatgacga ctccagcggc
      601 gacggcgctt ttgatcgcgt atctttgccg cagatcctcg ctaaggctcg aaaattccat
      661 cccgagggcc ttttcgaccg ggcccggcgg cgccttcatc ttggattcgc cctgcgttcc
      721 gccgagggaa gcctgttttt cattctcttc caaacggccg agcttaatca ttttggcgat
      781 cggtttgccc tgccgcatga cgacgatttc gacgtcttta ccgaccggcg ccgcggcgac
      841 gatcttgggc aggtcgcgcg attccttgat ttcggttcca tcgaatttga tgatgacatc
      901 gccggccttg atgccggccg ccttggccgg acctctgtcg tcggttccgg caacgagcgc
      961 acctcggagt ttgccaagat tgaggctttc cgcaattgtg tcgtcgatat tttggatacg
     1021 cacgccgagc caaccgcgcc gcgtctcgcc atatttctgc aattgctcaa tgacaggaat
     1081 gaccgtcgca gcgggcgttg cgaagccaat tccaatcgac ccgccgctcg gcgacaggat
     1141 cgccgtattc acgccaatga cttcgcctgc catgttaaac aatggcccgc ccgaattccc
     1201 tttgttgata gcagcgtcgg tttgaaagta gttgtcatag ggcccagagt cgatgttgcg
     1261 gtggcgagcc gaaatgacac ccgcagttag cgtgccgcct agcccaaacg ggttgccgac
     1321 ggcgaccacc cagtcgccca cacgcatctt ttcgctgtcg ccaaattttg ccgccttgag
     1381 aggcttttcc ggcttgaccc gcagtaccgc aacatcaacc ttttcatctt tgccgacaat
     1441 ttcagccttc agtttctggc cgtcgcttaa gatcacggtc acttcgttgg catcggcaat
     1501 gacgtggtaa ttggtgatga cgatgccgga tgcgtcgatg acgaaaccgg agccgagcga
     1561 atttgatttg cgttcgtgcc ttttttgatc ttgccgttcg ccgccgccct gttgatgccg
     1621 ccggaaaaac tcctcaaaga gatcgtcaaa aggtgttcca ggcccgagct ccggggcatt
     1681 gcccgcgtgc ttgtcatcga tcgtttgtgt cgcggagata tttaccactg aatcgctcac
     1741 cccatcggct aggtcggcaa gggattctgg tccttttgcg acggccgggg tggaaaatgg
     1801 cgtaacgctt ccggcaatgc ccagagcgag tgcgcagccg accaggaaag cagcgctagt
     1861 gctggttcgc cgctgcgcat cacctgcggc gttttcaatg ccgcgccata ccggaagtac
     1921 acccatgaaa cctatctcct ttaagcaaaa acgaatcggg cttggggatt cggatagaag
     1981 cgccgcaaat caccgaggat ccaccatctc tcatcgatcc ggcccgcctt ctactacgaa
     2041 tttgtcccaa actcattcga ctttttcaca agcgctctaa tcgcttcgtc cacgctgtga
     2101 aacaaatgcg tccgcggcag agcatcggtg atcccaaagc gctcaaaacc gtcctgggcg
     2161 cgcagtgatt cgaggcgggc gactgcgacg gaaatcttct gctgcgcgca atccttgatg
     2221 aaggaaagga gaatctgcgc cgcggtgaag tcaatgtcga taatgctgct tgcctccaaa
     2281 acgatgaggc ttggcggcgt ggggcattct cttatgacac gctgcacgcc ctcttggaag
     2341 gcgtaggcat tgagaaaaga gagcggcgcc tgaaaggcga taaccagaac gccatcaatc
     2401 gtccctccct ttgtgctacg gcccggcggc caccaaatcg aagtgccggg aaccctttcg
     2461 aaagcgacaa tttgcgggcg ggtcaccgac cacattccat gcagcaacga caacacgatg
     2521 ccgatcgcaa ctcctgttcc gaccggcagc aggataatcg cggcaaatgt cgtgaagata
     2581 aggaggaatt cgccgaaagc gctccgcgag acggctagga tctcacgcgg gcgcacaagc
     2641 ttgattgcga caaacatcag tgtgcctgca agtgcggcca tcggaatatt ggcaaggagc
     2701 gcggtgccgt aaacggcaag cacgagaata agcgtggcgg cggcaagccc ggcgagtttg
     2761 ctgcg
//
LOCUS       DATMPU010000117         2430 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_2261967,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000117 DATMPU010000000
VERSION     DATMPU010000117.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 2430)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2430)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2430
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2261967"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..647
                     /locus_tag="VNY06_02715"
     CDS             <1..647
                     /locus_tag="VNY06_02715"
                     /inference="COORDINATES: protein motif:HMM:NF025388.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4238 domain-containing protein"
                     /protein_id="HWX13776.1"
                     /translation="VLSMAWAHFFLCPPAQREIAKGIHANEVRAMARSLFEGHRLDPA
                     TLNIEIEREADFFKRFGTKHLASATWAFYNQDWIILLNETETPFITSDNPAAFVPNSK
                     PPERILPISPSLCLYAKIDLSIAETDHFDWGSPPNGSITFRNISRPSAMKINRFTAIN
                     ANDLVFAQSVDVGIAALVKKYRDVRPRVEPMRVALPADDGALKGAALTIGPRTK"
     gene            complement(690..1673)
                     /locus_tag="VNY06_02720"
     CDS             complement(690..1673)
                     /locus_tag="VNY06_02720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020174467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HWX13777.1"
                     /translation="MLSFHAAGKDGIEVAMDAETIPSAVSWIDAERPDPAEIAFLERT
                     LKIEVPTLETLSEIETSSRLRSDKDWLYLSIPMIHRTDGFMPALTPLGFVLSKDVLLT
                     IRFKHMKAFDDVLETISRCPAQAGGPGALVVISEVIVGHAADVLEGVGSDLDGLSEKI
                     FGTNAANGRRHKPREDGAQLRLVLRKVGRNGDLTSKIEDLLLGMARMVPYVAANAAPY
                     LEAGIRAKLKSLQRDIASLNDYETHLTDKIHFLLDATLGLTNIDQNNIFRILTVVSVI
                     GIPPTFFASMWGMNFKNIHEYDWTYGYEFGLFIIAVSALIPIIWFKWRGWW"
     gene            complement(1699..>2430)
                     /locus_tag="VNY06_02725"
     CDS             complement(1699..>2430)
                     /locus_tag="VNY06_02725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012590430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HWX13778.1"
                     /translation="IAAITAYAVAMGKTPIVVEDCPGFAVNRTLMPYLIAFLRLVHDG
                     VDFQEIDKAMETFGWPMGPAYLIDVIGMDISHHVVEIISAGFAPRMDAPFESAIQILL
                     GVGRLGQKNGRGFYKYRSDSKGRPRKEIDPETERLLAAGQPNGKIRIGEEKIALRMML
                     PLILEAARCVEDGIAASPGEVDMCLILGLGLPRYLGGALKYADYLGLKNVVDRADNWA
                     GLGPIYRPSERLRAMAATGDVFYPA"
BASE COUNT          539 a          699 c          670 g          522 t
ORIGIN      
        1 tcgtgctctc catggcttgg gcgcattttt ttctttgccc cccggcgcag agggaaattg
       61 ccaaaggtat tcatgcgaac gaagttcgtg ccatggctcg aagtctcttc gaaggccacc
      121 ggctcgatcc cgcgactctc aacattgaaa tcgaacgcga agctgatttc ttcaaaaggt
      181 ttggcacaaa gcacctcgcc agcgcaacgt gggctttcta caaccaagat tggataatcc
      241 tgctcaatga aaccgaaacg ccgttcataa cgagcgacaa tcctgcagcc tttgttccaa
      301 attcaaaacc gccagaaagg attctaccga tttcgccgtc actttgtctg tacgcgaaga
      361 tcgatttgtc gatcgctgag acggatcatt ttgattgggg atcaccgccc aatggatcaa
      421 tcactttcag gaatatttcg aggcccagtg caatgaaaat taaccgcttt accgctatca
      481 atgctaacga cttggtgttc gctcaatccg tggatgtcgg aatcgcagct cttgtaaaaa
      541 agtaccggga cgtcaggcct cgggtcgaac ccatgagagt cgctcttcca gccgatgatg
      601 gggctctaaa aggcgcggct ttgacgatcg gaccacgcac caaataaata acctcagtca
      661 ttatcccagt gcgatagcgc cttccgccct taccaccagc cgcgccattt gaaccatatg
      721 atggggatga gcgcgcttac ggcaatgata aacagcccaa attcatagcc ataggtccag
      781 tcgtattcgt gaatgttctt gaagttcatt ccccacatgc ttgcgaagaa tgtcggcgga
      841 atgccaatca cggagacgac tgtcagaatt ctgaaaatat tgttttgatc gatgttcgtc
      901 agccccaagg tggcatcgag caggaaatga atcttatcgg ttagatgcgt ctcgtaatcg
      961 ttgagggaag caatgtcgcg ttgcaggctt ttaagctttg cgcgtatgcc ggcttcgaga
     1021 tagggcgccg cattggccgc gacatagggc accatgcgcg ccatgcccaa aagcaggtct
     1081 tcgatcttcg aggtcagatc gccgttgcgt ccgaccttgc gcagcacgag acgtaattgc
     1141 gcgccatcct cacgcggctt gtggcgccgg ccgttggcgg cattcgtgcc aaagattttc
     1201 tcggacaggc catcgagatc gctaccgacg ccttccagca cgtcggcggc atgaccgacg
     1261 atcacttccg aaatgacgac caaggcgccg gggcctcccg cttgcgccgg gcaccgcgaa
     1321 atagtttcca gcacatcgtc gaaggctttc atatgcttga aacggatggt taggagaacg
     1381 tctttcgaca agacgaatcc aagcggtgtc agcgcgggca tgaaaccatc cgtccggtgg
     1441 atcatgggaa tgctgagata gagccagtct ttatcgctgc gcagccggct cgacgtttcg
     1501 atttcggaga gcgtttcgag tgtcggcacc tcgattttga gcgtgcgttc gagaaaagca
     1561 atttccgccg ggtccggtcg ctcggcgtcg atccaactta ccgcggacgg aatcgtctcg
     1621 gcgtccatcg cgacctcgat cccatctttg ccggcggcgt gaaagctaag catgcggcct
     1681 cgcgtatcgg tgaagagctt atgcggggta gaaaacatcc ccggtggcgg ccatggcgcg
     1741 caggcgttcg ctggggcggt agattgggcc aaggcccgcc cagttgtcag cgcgatcgac
     1801 gacgttttta agcccgagat aatcggcgta tttgagcgcg ccgcctagat agcgcggcag
     1861 gccgaggcct agaatgaggc acatatccac ctcccccggc gacgcggcga tgccatcctc
     1921 cacgcaccgc gccgcttcaa ggatcaacgg cagcatcatg cgcagggcga tcttctcctc
     1981 gccaatgcgg atcttgccat tgggttgccc ggccgcgagc aggcgctccg tctccgggtc
     2041 gatttccttg cgcggccggc ctttggaatc gctccgatat ttataaaagc cgcggccgtt
     2101 cttctggcca aggcgcccca cgcccaacaa gatttggatt gccgactcga aaggcgcgtc
     2161 catgcgcggc gcgaaccccg ccgagatgat ctcgacgaca tggtgcgaaa tatccatgcc
     2221 gatgacatcg atgagatagg ccgggcccat cggccagcca aaagtttcca tcgccttgtc
     2281 gatttcttga aaatcgacgc cgtcatgaac gagacgcaga aacgcgatga gataaggcat
     2341 cagcgtgcgg ttgacggcaa accccgggca atcctcgacc acgatcggcg tcttgcccat
     2401 ggcgaccgca taggcggtga tcgccgcaat
//
LOCUS       DATMPU010000118         2295 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_3505889,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000118 DATMPU010000000
VERSION     DATMPU010000118.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 2295)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2295)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2295
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3505889"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..369)
                     /locus_tag="VNY06_02730"
     CDS             complement(<1..369)
                     /locus_tag="VNY06_02730"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HWX13779.1"
                     /translation="MTLDGQRIVILGGSSGIGLATAQAAAKEDASVVIASSRKLRLDE
                     ALATLQAGAEGYALDLTKAVKVFFAGLGPFDHLVFTAGETLQLGRLADTDIAAARGFF
                     GLRYWGAYTAAKYGCGSIRPG"
     gene            complement(747..1973)
                     /locus_tag="VNY06_02735"
     CDS             complement(747..1973)
                     /locus_tag="VNY06_02735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003612326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX13780.1"
                     /translation="MVKIEIVPVNGFLRFAAFCKLPRLLYAGQHGFAPPLDVERWANF
                     GHKLNPHFNLVEAQEFLAKRDGQWAGRISAQVYRPEITPVSASRAQFGALDAIDDIEV
                     TRALTEAAEEWLRARGADRINGPFSPTINGEVGMLVEGFDARPMIFMPWNPPYLAGHL
                     EALGYQKARDLNSYRFEIENFGLDEKPRMLNREEWHDRLNLRPLKLDQLKKGETALMS
                     ELFNDGWSANWGFVPFTVEELDSTADVLKYIMSPEFGIVVELDGVPQSFAIALPNLHE
                     ITADLNGRLFPFGLLRAISRIRKHKFEAARIVLLGTRKPLQNSVMGGVILLAMIEEFR
                     RRGASASIKHLEVGWVLEDNMAMRRPIELFGGKVDKIHRVYEKRFNGQAARRSALPQR
                     SRAGARKDLSLRCRRK"
BASE COUNT          480 a          673 c          669 g          473 t
ORIGIN      
        1 gcccggtcgt atgctgccgc aaccgtattt ggctgccgta taggcgcccc aatagcggag
       61 gccgaagaaa ccgcgcgcag cggcgatgtc ggtgtcggcc aggcggccaa gctgcagcgt
      121 ctcgccggcg gtgaagacca gatggtcgaa aggaccgaga ccggcgaaga acactttcac
      181 cgccttcgta aggtcgagcg cgtagccttc cgcgccggcc tgcagggtgg cgagtgcttc
      241 gtccagccgg agcttgcggc ttgaggcgat cacgaccgat gcgtcttctt tcgccgcggc
      301 ctgtgccgtg gcgaggccaa tccccgagct gccgccaagg atcacgatgc gctgtccgtc
      361 aagtgtcata gtttctgtcc tttcgctgtg gtgcacaggc aattcgtcga aaattgaatt
      421 gccgctggcg cgtctttgtc catttccatg atgactgggg atcatcatag cttgcgatgg
      481 caggcgctcg gcggcaagac ggggaatttc gcgcgggccg ccgaaccgtt gggtctaacg
      541 cggttaggcg taagccgggc tgtagcagac tcaacggctt ggtccgcgtc tccatgacct
      601 catccggatg cgggtagttt gttttgacag ttcggggaac catcgcggtg acacgaagtc
      661 cgctttttgg cgcagcagaa ttcagcctga gacagggctt gcgacgccct caactcggcc
      721 aggggctgcc aaatgccagg ggcgactcat ttgcgtcgac accggagcga caaatccttc
      781 cgcgcgcccg cgcgggagcg ttgcggcagg gccgatctgc gcgccgcctg gccattaaag
      841 cgcttctcat agaccctgtg aatcttgtcg accttgccgc caaacagttc aatcggccgg
      901 cgcatggcca tattgtcttc aagcacccag ccgacttcga gatgttttat ggaggccgag
      961 gctccgcgcc ttcgaaactc ttcgatcatc gccaacagaa taacgccgcc catgacgctg
     1021 ttttgcagcg gctttcgcgt gccgagcaga acgatgcgcg cggcctcgaa tttgtgcttt
     1081 cggatgcgcg agatcgcccg cagcaggcca aagggaaaga gccgtccgtt gaggtcagca
     1141 gtgatctcat gcaagttggg aagcgcgata gcaaaggatt gagggacgcc gtcaagctca
     1201 acgacaatgc cgaactcagg cgacataata tatttgagca catcggccgt ggagtcgagc
     1261 tcctcgaccg tgaagggaac aaagccccaa ttggcgctcc agccatcgtt gaacagctcc
     1321 gacatgaggg ccgtctcgcc cttcttcaac tgatcgagtt ttagcggtct cagattcagg
     1381 cgatcgtgcc attcctcgcg gttcaacatg cgtggctttt cgtcgaggcc gaaattttcg
     1441 atctcaaagc gataggaatt aagatcacgc gctttctgat atcccagcgc ctcgagatgg
     1501 cccgcaagat agggcgggtt ccacggcatg aagatcatcg gccgagcgtc aaagccttcg
     1561 acgagcatgc ccacctcgcc attgatggtg ggcgaaaagg ggccattgat gcgatcggcc
     1621 ccgcgtgcgc gcagccactc ttccgcagcc tcagtcaatg cgcgcgtgac ctcgatgtcg
     1681 tcgattgcgt cgagcgcccc gaattgcgcg cgcgaggcgc taacgggcgt gatttccggc
     1741 ctatagactt gcgccgaaat gcggccggcc cattgcccgt cgcgctttgc caaaaactcc
     1801 tgcgcctcga ccaaattgaa atgggggttc aatttatgtc cgaaattggc ccaccgctcg
     1861 acgtcgagag ggggcgcaaa gccgtgttgt cccgcataaa gcaggcgcgg aagtttgcag
     1921 aaagccgcaa accgcaaaaa cccattgacc ggaacgattt cgattttcac tataagcaga
     1981 gctggtaaca gcccgcgcgc aaccccacaa gccggggctt tctttcggcg ccaaaaatcc
     2041 tagcaaaaaa taccctgcga aacatttttg ttcagccggg acccaaagtc tataccaact
     2101 ctcggggagt gtgacttgga ggtgaattcc gaaagctgtg caaatttgaa ttaacaaaaa
     2161 gggcgcggcg cctcgttttt tgccccctca cggcgatgga cttgcatctt gccgaaatca
     2221 caaaagagag cgcctagcct gctcctcccc tcggttggtt tcatactcgg attaaaaatc
     2281 cgggttagtt tttct
//
LOCUS       DATMPU010000119        10640 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_715707,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000119 DATMPU010000000
VERSION     DATMPU010000119.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 10640)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10640)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10640
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_715707"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..225
                     /gene="rplL"
                     /locus_tag="VNY06_02740"
     CDS             <1..225
                     /gene="rplL"
                     /locus_tag="VNY06_02740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008385454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HWX13781.1"
                     /translation="PAEEQTEFTVVLAAIGEKKIEVIKEVRAVTGLGLKEAKDLVEAA
                     PKPLKEGVTKEEAEKIKAALEKAGAKVEFK"
     gene            488..4609
                     /gene="rpoB"
                     /locus_tag="VNY06_02745"
     CDS             488..4609
                     /gene="rpoB"
                     /locus_tag="VNY06_02745"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010686894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HWX13782.1"
                     /translation="MAQTFTGRKRIRKFFGHIREVAEMPNLIEVQKASYDQFLLMEEP
                     QGERPDEGLQSVFKSVFPISDFSNTALLEFVKYEFEPPKYDVEECRQRGMTFAAPLKV
                     TLRLIVFDVDPETGAKSVKDIKEQDVYMGDMPLMTTNGTFIVNGTERVVVSQMHRSPG
                     VFFDHDKGKSHSSGKLLFAARIIPYRGSWLDIEFDAKDIVYARIDRRRKIPATSILYA
                     LGMDGEEILSIFYKSIAYTRDGDNWRLPFEPDRMKGMKAAVDLFDADTGEIALESGKK
                     LTLRTARLLSERGVKFLRAHDEDLHGQYIAADLYDAKTGEIFAEAGDEITAKSLAHLI
                     AAGFAELPVLDIDHVNVGPYMRNTLAVDKNKRREDALFDIYRVMRPGEPPTVETAEAM
                     FHSLFFDAERYDLSAVGRVKMNMRLDLDAPDTVRTLRKEDIIAVMRALVELRDGRGEI
                     DDIDHLGNRRVRSVGELMENQYRLGLLRMERAIRERMSSVDVDTVMPQDLINAKPAAA
                     AVREFFGSSQLSQFMDQTNPLSEITHKRRLSALGPGGLTRERAGFEVRDVHPTHYGRI
                     CPIETPEGPNIGLINSLATFARVNKYGFIETPYRRVKESRVTEDVVYLSAMEESKYYV
                     AQANAPIDSENTLTEDLVVCRHLGDVVMVPRERVDFMDVSPKQLVSVAAALIPFLEND
                     DANRALMGSNMQRQAVPLVRADAPLVGTGMESVVARDSGAAIAARRTGVVDQVDATRI
                     VIRASEETDASKPGVDIYRMMKFQRSNQSTCINQKPLVRVGDLVGKGDIIADGPSTDL
                     GDLALGRNVLVAFMPWNGYNFEDSILLNERIVRDDVFTSIHIDEFEVSARDTKLGPEE
                     ITRDIPNVSEEVLKNLDEAGIVYIGAEVQAGDILVGKITPKGESPMTPEEKLLRAIFG
                     EKASDVRDTSLRVPPGVQGTIVEVRVFNRHGVEKDERAQAIEREEIERLAKDRDDELA
                     ILDRNVYARLMDVLIGKKAVAGPKGFKRDTLLSKEVIEEHPRSQWWMFAVDNDRTMAE
                     LEAMRKQYDEAKKGLENRFLDKVEKLQRGDELPPGVMKLVKVFVAVKRKIQPGDKMAG
                     RHGNKGVVSKIVPQEDMPFLTDGQPVDIVLNPLGVPSRMNVGQILETHLGWACAGLGK
                     QVGEAVNVYLREKQTRPLRDRLIEIYDAKSQIMALDDETVVELGENLRRGVPVATPVF
                     DGARERDIVNMLESAGIDKSGQVTLYDGRTGEAFDRKVTVGYIYMLKLHHLVDDKIHA
                     RSIGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTLQEMLTVKSDDVAGRTK
                     VYESIVRGDDAFESGIPESFNVLVKEMRSLCLNVELNQTSRSHNQLPSAEAAE"
     gene            4723..8916
                     /gene="rpoC"
                     /locus_tag="VNY06_02750"
     CDS             4723..8916
                     /gene="rpoC"
                     /locus_tag="VNY06_02750"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012384345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HWX13783.1"
                     /translation="MNQEVMNLFSPAVQPQAFDQIQISIASPEKILSWSFGEIKKPET
                     INYRTFKPERDGLFCARIFGPIKDYECLCGKYKRMKYKGVICEKCGVEVTLSRVRRER
                     MGHISLAAPVAHIWFLKSLPSRIGLLLDMTLKDIERILYFESYIVLDPGLTPLKDRQL
                     LSEDEYLRAQDEYGQDSFTAVIGAEAVREMLRSMNLPKIAEALKVEIAESTSELKPKK
                     LAKRLKVIEAFMHSGNKPEWMVLTEVPVIPPDLRPLVPLDAGRFATSDLNDLYRRVIN
                     RNNRLKRLIELRAPDIIIRNEKRMLQEAVDALFDNGRRGRVITGANKRPLKSLADMLK
                     GKQGRFRQNLLGKRVDYSGRSVIVVGPDLKLHQCGLPKKMALELFKPFIYARLDAKGL
                     SATVKQAKKLVEKEKPEVWDILDEVIREHPVMLNRAPTLHRLGVQAFEPKLIEGKAIQ
                     LHPLVCAAFNADFDGDQMAVHVPLSIEAQLEARVLMMSTNNILHPANGQPIIVPSQDI
                     VLGLYYLSLMRDGEPGQGMLFSNMGEIEHALHAKVISLHTGVKGRAWTYDNDGQRVSK
                     IFDTTPGRMMLGQLLPRSKKISFDVVNKLMTKKEISNMIDIVYRNCGQKETVVFCDRV
                     MSLGFLEAFKAGISFGKDDMVVPETKPRIIADTSSLAKEYEQQYNDGLITQGEKYNKV
                     VDAWAKCSDKLAEEMMAHISTVQKDENGRDQPINSIYMMAHSGARGSPTQMKQLAAMR
                     GLMAKPSGEIIESPIISNFKEGLSVLEYFNSTHGARKGLADTALKTANSGYLTRRLVD
                     VAQDSIITANDCGSSSGIRMRAIIDAGQVVASLATRILGRTAAEDLKEADGKVIVASG
                     EMIEERHIDRINAAGIQEVKIRSVLTCEAKNGVCCKCYGRDLARGTPVNMGEAVGVIA
                     AQSIGEPGTQLTMRTFHIGGAAQIADQSFIESNFDGIVRIRNRHLARNGEGDLMVMAR
                     NVAIVIEGPSGAERAVHRIQYGARLRVDEGDKIKRGQRIAEWDPYTRPILTEIDGIVG
                     FEDLVEGASMTETIDEATGIAKRLVMDWRLNQRSATLKPAIAIKSPDGKIAKLPRGGE
                     ARYVLPVDSIIAVDPGGKVRAGDIIARISMESAKTRDITGGLPRVAELFEARRPKDHA
                     IIAEASGTVQFGRDYKNKTRISIIPNEEGADPIEYLIPKGKHIHLQDGDVIEKGDYIV
                     DGNPAPHDILAIKGVEELAAYLVNEIQEVYRLQGVSINDKHIEVIVRQMLQKVDIVEP
                     GGTDFLPGEQVDRIELDEANALAVAEGKKPASGTPVLLGITKASLQTRSFISAASFQE
                     TTRVLTEAAVNGKADSLEGLKENVIVGRLIPAGTGAAMAKFRRVANMRDQMILADKAE
                     SAAVVEPALLPQPEVPAGE"
     gene            9001..>10640
                     /locus_tag="VNY06_02755"
     CDS             9001..>10640
                     /locus_tag="VNY06_02755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012385135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24B family metallopeptidase"
                     /protein_id="HWX13784.1"
                     /translation="MFESLYQSFEDQADPSQGAARVAALRAELVRRGLDGFVVPRADE
                     HQNEYVAPCAERLAWLSGFTGSAGLAIVLKERAAIFVDGRYALAVRDQVDLSVFATVT
                     LTETSPQQWLETNLAGGMRLGYDPWLHTPSQLERFSKAAEAAGAELIAVEPNPIDSMW
                     SGRPLPPLGKITLHSRRFAGEVAKRKLGRVAAALGNNDALLVSDPHAVAWVFNIRGHD
                     VAHTPLPLSYALIFKNAEPRLYADARKLDNHTRHSLSALAEIEEPACLKRDLEILGGE
                     GKKLLLDSTSAPAKLAAVFKDAGGICELGTDPIAGMKACKNATELEGARNAHLRDGAA
                     MVRFLHWFAVHAPGGKLTEIDAARALETFRRDTQKLKDVSFPSIAAAGANSAIPHYRL
                     TVKTNAKIHKGIFLIDSGGQYEDGTTDITRTISVGKPTAQTRDRFTRVLKGHIAIARA
                     VLPKGTTGAQIDALARVPLWRAGLDFDHGTGHGVGSYLSVHEGPQRISKLGSAVLEPG
                     MILSNEPGYYRAGHWGIRIENLIVVERRAIAGEEREMLG"
BASE COUNT         2412 a         2837 c         3144 g         2247 t
ORIGIN      
        1 cctgctgagg agcagaccga attcactgtt gtccttgccg caatcggtga gaagaaaatc
       61 gaagtcatta aggaggtgcg tgcggtgacg ggacttggcc ttaaggaagc aaaagatctt
      121 gtcgaagccg cgcccaagcc cttgaaagaa ggggtcacca aagaggaggc tgagaaaatc
      181 aaagcagcgc tcgagaaagc gggcgccaag gtggagttta agtgattgca gcgttacggc
      241 agctgtcgtc ttttgagccc ggccgcgacg gtctgagaaa ggccgtcgcg gccgggccaa
      301 acccttgttt gcattgttat agaaacggtc cggacagttt atcactgcca ttcggtctct
      361 aatcatagat tgttggggct gagcaagtcc cgtcctccta gcgacatggc tggacgcggg
      421 ccgagcgggt gtccccgcga gaaattcgct gtatacacga ggtgcgagga gcgacatggc
      481 tcatagtttg gcgcagacgt tcaccggtcg caagcgtatt cgcaagtttt tcgggcacat
      541 ccgcgaagtt gcggaaatgc caaatttaat cgaagtccaa aaagcttcct atgaccaatt
      601 tcttctaatg gaggagccgc aaggcgagcg gcctgacgaa ggcttgcaat cggtctttaa
      661 gtcggttttc ccaatttctg atttttcaaa caccgcgctg ctcgagtttg ttaagtatga
      721 attcgagcca ccgaaatacg atgttgaaga atgccgccag cgcggaatga cctttgccgc
      781 gccactcaaa gtaaccttac gtctgatcgt cttcgatgta gatcctgaaa ctggcgcgaa
      841 gtcggtcaag gatatcaagg agcaggatgt ctatatggga gacatgccct tgatgacgac
      901 gaacggtacg ttcatcgtga acggaaccga gcgggtcgtc gtttcgcaga tgcatcgttc
      961 gcccggcgtt tttttcgatc acgacaaggg aaaaagccac tcctccggca agctcttgtt
     1021 cgcggcccgg attatccctt accgtggttc gtggctcgat atcgaattcg acgccaagga
     1081 tattgtttat gcgcgcatcg accgccgcag gaagattccc gcgacttcaa ttctctatgc
     1141 ccttggcatg gatggcgaag agattctttc aatattttat aagtcgattg cttacaccag
     1201 ggatggtgac aactggcgtc tacccttcga gccggatcgc atgaaaggca tgaaggcggc
     1261 ggtcgacctc tttgatgctg acaccggtga gattgcgcta gaatccggca aaaaactaac
     1321 cctaaggaca gctcgccttc tgtcagagcg gggagtgaaa ttcctgcggg cgcatgacga
     1381 agatctgcac gggcagtaca tcgccgcgga tctctacgac gccaaaacgg gcgaaatctt
     1441 tgccgaagct ggcgatgaga tcaccgccaa gtcgttagcg catttgatcg cagcgggttt
     1501 tgcggagctg cccgtgctcg acatcgatca tgtcaatgtc ggcccctaca tgcgcaatac
     1561 acttgccgtc gacaagaaca agaggcgtga ggacgccttg ttcgacatct accgcgtgat
     1621 gcgtccaggt gaaccaccga ccgttgaaac ggcggaggcg atgtttcatt cgctgttttt
     1681 cgatgcggaa cgctatgatc tctcggcggt cggccgcgtg aagatgaaca tgcgcctgga
     1741 tcttgatgcc ccggatactg tgcgcacgct gcgcaaggaa gacatcatcg cggtcatgcg
     1801 agcgcttgtc gaactacgcg atgggagggg tgagatcgac gacatcgacc atctcggtaa
     1861 ccgccgcgtg cgctcggttg gcgagctcat ggagaatcaa tatcggctcg gtttgctgcg
     1921 gatggagcgg gcgatcaggg aacgaatgtc ttccgtcgat gtcgacactg tcatgccgca
     1981 agacctgatc aatgccaaac cggctgcggc cgcggtacgc gagttttttg gctcgtcgca
     2041 attgtcgcag ttcatggatc agacaaaccc cctttccgaa atcacgcata agcggaggct
     2101 gtcggcgctt ggcccggggg ggctcacccg cgagcgagcc ggtttcgagg tgcgtgatgt
     2161 gcatcccacg cattacggac gtatttgccc catcgaaacg ccggaaggcc caaatatcgg
     2221 cctcatcaat tcgctcgcca ccttcgcgcg ggttaacaaa tatgggttta tcgaaacgcc
     2281 ctaccgccgg gttaaagaga gccgcgtgac cgaggacgtg gtctatcttt cggctatgga
     2341 agaatccaaa tattacgtcg cgcaagcgaa tgcgccgatc gactccgaaa atacgctgac
     2401 ggaagatttg gttgtctgcc ggcacttggg tgacgttgtc atggttccgc gcgaacgcgt
     2461 cgatttcatg gatgtttccc ctaagcagct ggtttcggtt gccgcagccc tcattccttt
     2521 tcttgagaat gacgacgcga accgagcgct catgggctca aacatgcagc gtcaggcagt
     2581 cccgttggtg agggcggatg ccccgcttgt cggaacaggc atggagtcgg tggttgcgcg
     2641 ggactcaggc gcggcgatcg cggcacgcag gaccggcgtt gtcgaccaag ttgacgcgac
     2701 gcgcattgtc attcgggcga gcgaggaaac cgacgcaagc aagcccggcg ttgatatcta
     2761 ccggatgatg aagtttcaac gttcgaatca atcgacctgt atcaaccaga agcctctggt
     2821 ccgcgttggc gatttggtcg gcaaaggtga catcatcgcc gatggcccat cgacggatct
     2881 tggcgatctc gcgcttggcc gaaatgtact cgtcgctttc atgccgtgga acggctataa
     2941 ttttgaggat tccatcctgc tcaacgaaag aatcgtgagg gatgacgttt tcacctcgat
     3001 ccatatcgac gaatttgagg tgagtgcgcg cgataccaag cttggcccag aggaaatcac
     3061 gcgcgacatt ccgaacgtat cggaagaagt tttgaagaac ctcgacgaag ccggaattgt
     3121 ctatatcggt gccgaggtcc aggcgggcga catattggtc ggcaagatca cgccgaaggg
     3181 tgaaagcccg atgacgccgg aagaaaaact tttgcgcgcg atatttggcg aaaaggcgtc
     3241 ggatgtgcgc gatacctcgt tgcgggttcc gcccggcgtt caaggtacca ttgtcgaggt
     3301 acgtgtcttc aaccggcacg gtgttgaaaa ggacgagcga gcgcaggcga tagaacgcga
     3361 agaaatcgag cgcctcgcca aagaccgtga tgatgagctt gccatccttg accgcaatgt
     3421 ctatgcgcgc ctgatggatg ttcttattgg caagaaggcc gtcgccgggc cgaagggttt
     3481 taagcgcgac actttgctca gcaaggaggt catcgaagaa catccccgct cgcaatggtg
     3541 gatgtttgcc gtcgataacg accggaccat ggccgagctt gaagccatgc gcaagcaata
     3601 tgacgaggcg aagaagggcc ttgagaaccg ctttctcgac aaggtcgaaa agttgcagcg
     3661 aggtgatgaa ttgccgccag gggttatgaa gctcgtcaaa gtattcgtcg ccgtgaagcg
     3721 caagattcag ccgggcgata agatggcggg gcggcacggc aacaaggggg tcgtctccaa
     3781 gattgtgccg caggaagaca tgccatttct aacggacggt caaccggtcg atattgtgtt
     3841 aaatcccctt ggcgtgccat cccgcatgaa tgtcgggcag atcctcgaga cccatctcgg
     3901 ctgggcctgc gccggattgg gtaagcaggt gggcgaggcg gtgaatgtct atttgcggga
     3961 gaaacaaacc aggcccctgc gtgataggct cattgaaatc tacgacgcca aatcacagat
     4021 tatggcgctc gatgacgaaa ccgtggtcga acttggcgaa aatctgcgcc gcggtgtgcc
     4081 ggtcgcgaca ccggttttcg atggcgcgcg cgaacgggat atcgtcaata tgctcgagtc
     4141 cgcaggcatc gacaaatctg gtcaggtcac gctctacgat gggcgtacgg gggaagcttt
     4201 cgaccgaaag gtgaccgtcg gctatatcta catgcttaag ctgcatcatc tcgtcgatga
     4261 taagatccat gcgcgttcga tcgggcctta cagtcttgtc actcagcagc cgcttggcgg
     4321 caaggcacaa tttggcggcc agcgcttcgg cgagatggaa gtctgggcgc ttgaggctta
     4381 tggagcggcc tacactttgc aggaaatgct gactgtaaaa tccgatgacg ttgccggccg
     4441 tacaaaggtt tatgaatcga tcgtacgcgg cgatgacgct ttcgaatcgg gcattcctga
     4501 aagtttcaat gtgctcgtta aggagatgcg ttcgctttgc ttgaatgttg aacttaatca
     4561 aacttcgaga tctcacaatc agctgccctc ggccgaggct gccgaatagg ttcgagcttc
     4621 ggcgattgcg ttgcagccaa agccgccccc gcgcggcatc attcaaatgt ttccggcaac
     4681 aggcctgcgg cggcgcgcat ggtctaggat caggagatat caatgaatca agaggtcatg
     4741 aatcttttca gcccggcagt gcagccgcag gctttcgatc agatccagat ttctatcgct
     4801 agccctgaaa agatactgtc ctggtccttt ggcgagatca agaagccgga gacgatcaat
     4861 tatcgcacgt ttaagcccga acgggatgga ctgttctgcg cccggatttt tggcccgatc
     4921 aaggactatg agtgtctgtg cggcaaatat aagcggatga aatataaggg tgtcatctgc
     4981 gaaaaatgcg gcgttgaggt taccttgtcg cgggtccggc gtgagcgcat ggggcatata
     5041 tcgctcgccg cgcccgttgc gcatatctgg tttttaaaat ctctgccgtc gcgcatcgga
     5101 ttgctgcttg atatgacgtt gaaggacatc gagcgcatcc tctatttcga atcctatatc
     5161 gtgcttgatc cggggcttac accgctcaag gaccgccaac ttctctctga ggacgagtat
     5221 ctgcgtgcgc aggatgaata tggccaagat tccttcaccg ctgtcattgg tgccgaagcg
     5281 gtgcgcgaaa tgctgcgtag catgaatctg cccaagattg ccgaggcgct caaggtcgag
     5341 atcgccgaat cgacgtcgga attaaaaccc aagaagcttg caaaaaggtt gaaggtcatc
     5401 gaggccttca tgcattcggg caacaagccg gaatggatgg tcctgaccga agttccagtc
     5461 attccgcctg atcttaggcc gctcgtgccg cttgacgccg gccggtttgc cacctcggac
     5521 ctcaatgacc tctaccggcg cgtgatcaac cgcaacaacc gtttgaaacg gcttattgaa
     5581 ctgcgggcgc cggatattat catccgcaac gagaaacgca tgttgcagga ggcagtcgac
     5641 gcgctatttg acaatggccg gcgggggcgg gtcattaccg gagccaacaa gcgcccgctc
     5701 aagtcgcttg ccgacatgct taagggaaag cagggccgct tccggcaaaa tttgcttggc
     5761 aagcgcgtcg actattccgg ccgttcggtg attgtggttg ggccagatct caaactccat
     5821 caatgtggtt tgccaaagaa gatggcgctc gagctcttca agcccttcat ctatgcgcgg
     5881 cttgatgcaa aagggctgtc tgcaaccgtc aaacaagcga agaaacttgt cgaaaaggaa
     5941 aagccagaag tctgggatat cctcgatgaa gtgattcgtg agcatccggt gatgttgaac
     6001 cgggcgccga cgctccaccg tctcggtgtt caggcttttg aaccgaaatt gattgaaggt
     6061 aaggccatcc aattgcatcc gctcgtttgt gctgcgttca atgcagattt cgacggcgac
     6121 cagatggcgg tacacgtccc gctctctatc gaggcgcaac tcgaagcgcg ggtgttgatg
     6181 atgtcgacca acaacattct tcacccagcc aacggccagc cgatcattgt gccatcgcag
     6241 gatattgtgc tcggccttta ttatctctcg ctaatgcgcg acggcgagcc gggacaggga
     6301 atgttgtttt cgaatatggg tgaaatcgag catgcgttgc atgccaaggt cattagcctg
     6361 catacgggcg tcaagggcag ggcatggacc tacgacaacg acggccagcg ggtgtcaaaa
     6421 atattcgata ccacgcccgg ccggatgatg cttggccaat tgcttccgcg cagcaaaaaa
     6481 atttccttcg atgttgtgaa caagctcatg accaagaaag aaatttcgaa catgatcgac
     6541 attgtctacc gcaattgcgg gcagaaggag acggtcgttt tctgcgaccg ggtcatgtcc
     6601 cttgggttcc tcgaggcctt caaggccgga atttccttcg gcaaggacga tatggttgtg
     6661 cccgagacca agccgcgcat tatcgcggat acaagttcgc tcgccaagga atatgagcaa
     6721 caatataatg acggactgat cacccagggc gagaaataca acaaagtcgt tgatgcgtgg
     6781 gccaaatgct cggacaagct tgccgaggaa atgatggccc acatctccac agtgcaaaaa
     6841 gatgagaacg gccgcgacca gccgataaac tcgatctaca tgatggcgca ttcgggcgcc
     6901 cgcggatcgc caacgcagat gaaacagctc gccgcaatgc gcgggctgat ggcgaagccg
     6961 tcgggcgaaa tcatcgagag cccaatcatc tcgaacttca aggaaggtct aagcgtgctc
     7021 gagtatttca actcgacgca cggcgcgcga aagggtcttg ccgacacggc cttgaaaacc
     7081 gcgaattccg gttatctgac ccgccggctt gtcgatgtcg ctcaggactc gatcattacg
     7141 gcaaatgatt gcggatcgag cagcgggatc cgcatgcggg ccatcatcga cgcagggcag
     7201 gttgttgcct cgctcgcaac gcgtatcctt ggccgcacgg ccgctgagga tttgaaagag
     7261 gccgacggca aggtcatcgt ggcctcagga gagatgattg aagaacggca tattgatcgg
     7321 atcaacgccg ctggaattca ggaagtcaag attcgttcgg tcttgacttg cgaggccaag
     7381 aacggcgtgt gctgcaaatg ctatggccgg gatctcgcgc gcgggacgcc ggtcaatatg
     7441 ggtgaggccg tcggcgtgat cgccgcgcag tcgattggcg agccaggaac gcagcttacc
     7501 atgcgcacct tccacattgg gggtgcggcg caaatcgccg atcaatcctt catcgagtcc
     7561 aattttgatg gaatcgtgcg gattcgcaat cggcatcttg cacgcaacgg cgaaggcgat
     7621 ctgatggtca tggcccgcaa tgtcgcgatt gtgatcgagg ggccgagcgg cgcagaacgc
     7681 gcggttcaca gaatccaata tggcgcgagg ctcagggtcg atgaaggcga caagatcaaa
     7741 cgcggtcagc gaattgccga gtgggatccc tacacgcgcc cgattttgac cgaaatcgat
     7801 gggatcgtcg gcttcgaaga cctcgtcgag ggtgcctcca tgaccgaaac gatcgatgag
     7861 gcgacaggca tcgccaagcg cctggtcatg gattggcgcc tcaaccaacg ttcggcgaca
     7921 ttaaaaccag cgatcgcgat caaatctcca gacggcaaga ttgcgaagct gccgcgcggc
     7981 ggtgaagcgc gctatgtctt gccagtggac tcgatcatcg ctgttgaccc aggcggcaag
     8041 gtaagggccg gcgatattat cgcgcgcatt tcgatggaat cggctaagac gcgcgacatt
     8101 acgggcggcc ttccgcgagt tgccgaattg ttcgaggccc gccggccaaa ggatcatgcg
     8161 attatcgccg aggcttctgg cactgtgcaa ttcggacgtg attataaaaa taagactcgc
     8221 atatcgatca ttccaaatga ggagggcgcc gaccccatcg aatatctcat tccgaagggc
     8281 aagcacatcc acctccagga cggcgacgtt attgaaaagg gcgactacat cgtcgatgga
     8341 aatcccgcgc cgcacgatat cttggcgatc aaaggtgtcg aggaactcgc tgcctatctc
     8401 gttaacgaga ttcaagaggt ctaccggctg caaggtgtca gcatcaacga caagcatatc
     8461 gaggtgattg tcaggcagat gttgcagaaa gtcgatatcg tcgagccggg tggcaccgac
     8521 tttctaccgg gcgagcaagt cgaccggatc gagctcgacg aagccaacgc tttggcggtc
     8581 gcagagggaa agaaaccggc gtcgggaact ccagtgcttc tcggcattac caaggcctcg
     8641 ctgcaaacgc gctccttcat ctcggctgcg tcgttccagg aaacaacgag ggttctgacc
     8701 gaggcggccg tcaatggaaa ggcggacagt ttagaagggc tgaaagagaa tgttattgtc
     8761 ggtcgcctta tccctgccgg gaccggcgcg gcgatggcca aattccgccg tgtggccaat
     8821 atgcgcgatc agatgatttt ggcggacaaa gccgaaagcg cggccgtggt agaaccagcc
     8881 ttactgccgc aaccggaggt gccggctgga gaataggaca tccgccaggc agcctgttta
     8941 ccacgcgcct caatagatgc ccgggttgcg aggcgcgtga actcgcccta gtcttggctc
     9001 atgttcgaaa gtctttatca gtccttcgag gatcaggcag atccgtcgca aggagcggcg
     9061 agggttgcgg ccttgcgggc cgagctcgtc cgccgcgggc ttgatggatt cgttgtacca
     9121 cgcgcggacg aacaccagaa tgaatatgtc gctccttgcg cagagcggct ggcgtggctt
     9181 tcgggtttta ctggttcggc tggcctcgcg atcgtgctca aagagcgcgc ggccatcttt
     9241 gtcgatggac gctatgcgct cgccgtccgg gatcaggtcg acctttcggt cttcgcaacg
     9301 gtgacattga ccgagacaag ccctcagcaa tggcttgaaa ccaatctggc cggcgggatg
     9361 cggctcggat atgatccttg gctgcatacg ccgtctcaat tggagcggtt cagcaaagcc
     9421 gcagaagcgg cgggcgcgga acttatcgcc gttgagccca atccaatcga cagcatgtgg
     9481 agcgggcggc cgttaccgcc gcttggcaaa atcaccttgc atagccgccg gtttgccgga
     9541 gaggtagcca agcggaaact cggtcgagtc gctgccgcgc tcggcaacaa tgacgcgctg
     9601 ctcgtcagcg atccacatgc ggttgcctgg gttttcaata ttcgtggcca cgatgtcgcg
     9661 catacgccac tgccgctcag ctatgcttta atctttaaaa acgccgagcc acgtctttat
     9721 gccgacgcgc gcaagctcga caaccacacg cgccactcgc tttctgccct tgccgaaatc
     9781 gaagagcctg cctgtctcaa gcgtgatctt gaaattttgg gcggggaggg caagaagctt
     9841 ttgctcgatt ccacgtcagc ccccgccaag ctcgcagccg tcttcaaaga cgcaggcggc
     9901 atttgcgaat taggcaccga tccgatcgcc gggatgaagg catgtaagaa tgcgacggaa
     9961 cttgaaggcg cgcgcaatgc gcatctgcgc gatggtgcgg cgatggtgcg ctttctacac
    10021 tggtttgcag tccatgcgcc gggtggaaag ttgaccgaaa tcgatgccgc ccgcgctctc
    10081 gaaactttcc gccgcgatac gcaaaagctg aaagatgttt cattcccgtc aattgccgca
    10141 gctggcgcaa attccgcaat tccacactat cgcttgacag tcaaaaccaa cgccaaaatc
    10201 cacaaaggca tattccttat agattcgggc ggccagtatg aagatggcac gaccgacatc
    10261 acgcgcacga tttccgtcgg caagccgacc gctcagacgc gcgaccgctt tacccgtgtc
    10321 ctcaagggcc atatcgccat agcccgtgct gtccttccca aaggcaccac gggggcgcag
    10381 attgacgcgt tggcgcgtgt gcccttgtgg cgggcgggtc ttgatttcga ccatggcacg
    10441 ggccatggcg ttggctctta tctgtcggtg catgaaggac cgcagcggat ttccaagctg
    10501 ggatccgccg tccttgaacc tggcatgatt ctttccaatg agccgggcta ttaccgcgcc
    10561 gggcattggg gaattagaat cgaaaatctc attgtggttg aacgaagggc catcgcgggg
    10621 gaagagcgtg agatgctggg
//
LOCUS       DATMPU010000120         5045 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_2263466,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000120 DATMPU010000000
VERSION     DATMPU010000120.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 5045)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5045)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5045
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2263466"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(115..312)
                     /locus_tag="VNY06_02760"
     CDS             complement(115..312)
                     /locus_tag="VNY06_02760"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX13785.1"
                     /translation="MLERFGRLDEALACFDAIEALTPGDSANLVLQKLGRHKEALAAY
                     EEAARRGPPDADTEQGMFSKD"
     gene            complement(327..704)
                     /locus_tag="VNY06_02765"
     CDS             complement(327..704)
                     /locus_tag="VNY06_02765"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HWX13786.1"
                     /translation="MSVLPREQLSSAVNNDEFRNKTLLQIAVTEAIALLNGGKGQEAV
                     SQIVQYADIAAQSCVGCYVFGLIHFNAGKLRDALSWLNRALALQPAFVDALSARAVVH
                     QRLGQPQEALKSFEAVLKLRPGR"
     gene            1124..2113
                     /locus_tag="VNY06_02770"
     CDS             1124..2113
                     /locus_tag="VNY06_02770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020173617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extensin family protein"
                     /protein_id="HWX13787.1"
                     /translation="MARHVLPFAASAALTLALAGCSNYETPRRPAWRGAAEKACLAQK
                     PFQVSAYIQPAREIDGPGICGLFYPFRVSALRDGGVRLNSVYTLDCPMIARLNAWVSE
                     IMQPAARARFGQEVVEIDAMGAYSCRTMNNRPGGRISEHAFGNAIDIGGFRLADGREI
                     TIVHDWTRGDEQTRAFLQDVHSGACGQFTTVLGPGSNIFHYNHIHVDLALHGNTSSGP
                     RRICRPLLGPGPVPEPPRDGLPNPPEIEEEIDIAESRNPTGETLVMHPGPGQGPPARI
                     PDSLFADAARIPTARAYAPLPPEPVGAPFRPKSAHVPEGRPSDWDLTSSIPAR"
     gene            2552..2890
                     /locus_tag="VNY06_02775"
     CDS             2552..2890
                     /locus_tag="VNY06_02775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX13788.1"
                     /translation="MPQGDIEETKASALLPNLKIEILHGRLPTGDAERLTISVLAVPS
                     FEAFGRYLEAANPFLFWMRFAQTAWAPWLSAFPASTPRSALGRLSPAAQIVASSPPSK
                     RDGKSNSDAK"
     gene            complement(3032..4147)
                     /locus_tag="VNY06_02780"
     CDS             complement(3032..4147)
                     /locus_tag="VNY06_02780"
                     /inference="COORDINATES: protein motif:HMM:NF016196.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF445 domain-containing protein"
                     /protein_id="HWX13789.1"
                     /translation="MRRCGIIKELYPGGVPNQVTEPNVNLCQRVSVKLKEARLPNVSD
                     DTILRAAGYRVTRSALVRPWEFISLNFLAPEEVAAKLERIDAADWISRWLKEPENVKL
                     AARRLHGSLRPLLEFLREEQIRTFSRGVIRNGIDSIAAAPLAARVLSVLAAHGHHDAV
                     FDFAIETAQKFLSDNRETFRQRVAKNSIYWLPNWVDAKLADAFLAALLDTLDGACAAD
                     GPVRLQYRAALNRLVTRLAKDPEMFDQAERLKADVLDNSVVDSYLDWLSTEIEERIKA
                     EAAAPDGLFSSGLEHSLLTLGRWLESDEHIHAMINRWARQLVLSTVVPNRGEIGTFVA
                     EVVAKWDTMTLVEKLELQVGRDLQSVSMEPWSAGSSD"
     gene            4128..4598
                     /locus_tag="VNY06_02785"
     CDS             4128..4598
                     /locus_tag="VNY06_02785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX13790.1"
                     /translation="MMPHLRIEHRVLPAGPTICDMIANAALYLGLVRYVALSGHGDAG
                     GLPYADALHNFYAAARHGLDATIAWPSTGAIAADVLLLELLIPKAREGLVDLGIDDSE
                     GSFLDIVEARVRSRQTGAPWQRKAFEARGRDMYKLMAAYCERQRSGAPVHQWEL"
BASE COUNT          988 a         1519 c         1474 g         1064 t
ORIGIN      
        1 cttgcggccg ggacagcgcc tgcaaagcta ttcctttgtt gtagagggcc tgcgcataac
       61 cgtcgcgata ttgtgaggca agatcgtagg cggcgagcgc ttcctctagg cgtcctagtc
      121 tttggagaac attccctgct ccgtgtctgc gtccggcggg ccgcgccgcg cggcctcttc
      181 atacgcagcg agcgcttctt tatggcggcc aagcttctgc aagaccaagt tcgcgctatc
      241 tccgggagtt aaggcctcga tcgcatcgaa acaggccagg gcctcgtcga gccgtccgaa
      301 tcgttcaagc aatgcgccgc gattggtcag cgccctggcc gcagtttgag gactgcttcg
      361 aaggatttga gagcctcctg cggctgacca aggcgctgat gaacgaccgc ccgagcggag
      421 agcgcatcaa caaaggctgg ttgcaatgca agtgcacgat tcaaccagga caatgcatcg
      481 cgcagcttcc cggcattgaa atgaatgagc ccaaagacat aacagcccac acagctctga
      541 gcggcgatat ccgcatattg cacaatttgg ctgaccgcct cttgcccctt gccgccattg
      601 agcaaggcaa tagcctctgt gacggctatt tggaggagcg tcttgttgcg gaactcatca
      661 ttgttgacgg cactgctgag ttgttcgcgt ggaaggacgc tcatttaccc gtgctcatca
      721 ggatccggat cacccagccg gacccgcggt ttagcggcag gctagctggt tccaccgtga
      781 ggtttcaaaa ctgtcgaggc gagcacgacg ccgatcgcgc ccggactcga catgaaagat
      841 tggttttgga ttgaagcgaa acaaactttc gcgggcctga cggccatctc ctgcgccaca
      901 aaccgcctca cctgtttacg agcaagcacc gcggttgcga acctgggtct atgatcgcaa
      961 gacgccgatc accgcggccg atctcttgaa tgaccgggtg ctgatgttcg cagctgcccg
     1021 aaagacgccc ccaagtgcag gttttgccct tgcgatgcgc ggtttgaaac cgttaaccgc
     1081 aatgctttaa ggccagttaa gatattgcgc gcatgtttta gcaatggcgc gtcacgttct
     1141 gccatttgcc gctagcgcgg ccctgacgct ggcgttagcg ggctgctcaa actatgaaac
     1201 gccgcgccgg ccagcttggc gcggcgcggc agaaaaagct tgtctcgccc aaaagccttt
     1261 tcaggtttcg gcttatattc agcccgcgcg cgagatcgac ggcccgggta tttgcgggct
     1321 tttttacccc tttagggtaa gcgcgctgcg cgatggcggg gtgcgcctta attcggtcta
     1381 tacacttgac tgcccgatga ttgcgcggct aaacgcgtgg gtgagtgaga tcatgcagcc
     1441 cgccgcgcgg gcgcgtttcg gccaggaagt ggtcgagatt gacgcgatgg gagcctacag
     1501 ctgccggacg atgaataatc ggccaggagg acgcatttcc gaacatgcgt ttggcaatgc
     1561 gatcgatatt ggcggttttc gtctcgccga tgggcgcgag ataaccatcg tacatgattg
     1621 gacgagaggc gacgaacaaa cgcgggcttt tttgcaagac gttcactccg gcgcgtgcgg
     1681 ccaatttacg accgtgctcg gccccgggtc taacatcttt cactataatc atattcatgt
     1741 cgatctcgcg ctccacggaa acacttcttc cggcccgcgc cggatctgcc gccccttgct
     1801 cgggccaggc ccggttcccg aacctccgcg ggacggtcta cccaatccgc ctgagatcga
     1861 ggaggaaatc gatattgccg aaagcaggaa tcccacgggc gaaacgctcg ttatgcaccc
     1921 cgggccgggt caagggccgc ctgcacgcat tcccgatagc ctctttgcgg atgccgcacg
     1981 gatacctacg gcccgcgcat atgcgccgct ccccccagaa ccggtcggag cgccattccg
     2041 cccaaagtcg gcccatgttc cggaaggtag accgtccgac tgggatctca catcctcgat
     2101 tcctgcgcga tagagcgggg cctgcgccgg ctcctccttt ggctcggcgc acagaccagg
     2161 tagccgcgtg gccagtgttt gacctgccac gcggctgcgc cggggaggga gagattcggc
     2221 gttctcgtcc ggagggacgt ttaagtgtct gccgccgagg aggttcctcc ccccggctgc
     2281 acagtttggc tccgcgccaa ggccctgacg tcacctcggg tactataatg agcaatcggg
     2341 gggcgcatcc cgacagtctc tttccgggaa acgcaacgcg cctggaccgt ggacaaagtg
     2401 gagggcgcgc cggtttcctt ggcgggccga gccgtagggc ggcggcgcgt ggcgtagtgg
     2461 cggcgaagct ggtatgttgg ccgtcaaacc ttcctttgga gggcgctggc tcagtcctgc
     2521 cttcctaccg gctcaaaata tggagagaga gatgccgcaa ggcgatatcg aggaaaccaa
     2581 agcctccgcg cttttgccaa accttaagat cgaaatcctt catggccgct tacccaccgg
     2641 ggatgctgag cggctaacga ttagcgtgct ggcggtccca tccttcgaag catttggacg
     2701 ctatttggaa gccgcaaatc cttttctgtt ctggatgcgt ttcgcacaga cggcatgggc
     2761 gccctggctt agcgcttttc cggcatcaac gccgcgaagc gcccttggcc ggctttcccc
     2821 ggcagcgcaa attgtggcgt cttcaccccc ctcaaagcgc gacggcaaaa gcaattctga
     2881 tgccaagtga tgaggcgcga tagcgggagt cccggggcgt gcgatgtgtt ggcacaattc
     2941 tactcagtgc agggcaggtt tctatttggg gccggcttcg ttgaccattc atttggcctt
     3001 taaccgaagg cctgggtcac tgcgaaaatg gtcagtccga cgagcccgcc gaccagggtt
     3061 ccattgatac ggattgcaga tccctgccga cctgaagttc gagcttctcc accagcgtca
     3121 tggtatccca cttcgcgacc acctcagcga caaaggttcc gatctcccca cgattaggca
     3181 cgactgtgct caaaacgagc tgccgcgccc aacgattgat catggcgtgg atgtgctcat
     3241 cactttctaa ccatcggcca agagtgagca gcgaatgttc caaaccgctg gaaaagagtc
     3301 cgtccggagc agctgcctca gccttaattc tttcttcgat ctcggtgctc aaccaatcga
     3361 ggtagctatc cacgaccgaa ttatcgagca catcagcttt gagccgctca gcttgatcaa
     3421 acatctccgg atccttggcc aagcgggtga ccaggcgatt gagagccgcc cgatactgga
     3481 gtcgaactgg gccgtccgca gcgcatgccc cgtcgagggt gtcaagcaga gccgccaaaa
     3541 aagcgtcggc cagctttgcg tcgacccaat tcggaagcca gtagatgctg ttctttgcca
     3601 ctctctgccg gaaggtctcc cggttgtcac tgaggaactt ctgcgccgtc tcgattgcga
     3661 aatcgaacac tgcgtcgtgg tgaccatgcg ccgccaatac tgacagcaca cgggcggcca
     3721 agggtgccgc cgcgatcgag tcaatgccgt tacggatcac gccgcggcta aaggtacgga
     3781 tctgctcctc gcgcaaaaac tcgagcaaag gccgcaatga gccatgtaag cgccgcgccg
     3841 ccagcttgac gttttcaggc tccttgagcc agcgggaaat ccagtcggcc gcatcaatcc
     3901 tttcgagctt cgccgccact tcctctggag cgaggaagtt aagggagata aactcccagg
     3961 gcctcaccaa tgcgcttctt gtgacgcggt aaccggcggc tctcaaaatt gtgtcgtcag
     4021 aaacgttcgg cagcctggct tccttgagct tcacactgac tcgctggcat aggtttacgt
     4081 tgggttcagt gacttggttg ggtacgccgc cgggataaag ctccttgatg atgccgcatc
     4141 tacgcatcga gcatcgggtc ctcccggcag gcccgaccat ttgcgatatg atcgccaatg
     4201 cggcgcttta tttaggcttg gtgcgatatg ttgctctatc cggccacggc gacgcaggcg
     4261 gtcttcctta tgccgatgcc ttacacaatt tctatgcagc agctcggcat ggcctcgacg
     4321 caacgatagc atggcctagc acaggcgcta tcgccgctga cgttcttctc ctcgaactgc
     4381 tcattccaaa ggcgcgagag gggttggtgg atctcgggat agatgacagt gagggcagct
     4441 ttctggatat cgtcgaggcg cgggttcgtt cgcgacagac cggcgcccct tggcaacgca
     4501 aggcctttga ggcgcgagga cgcgatatgt acaaattaat ggccgcctac tgcgaacgtc
     4561 agcgcagcgg cgcgccagtg caccaatggg aactgtgagg cgatcgtgac cgagctgaaa
     4621 tgcgccgtct tcgagtcgat tccagactgc tttcttgact gccccgcagg gcgccttgct
     4681 gaactgcttc ctgggccgtg cctcattgat cttcccggac gcgagcggcg ccctttattc
     4741 gtttccgttt tgttgcatgg cagtgagcac actgaggccg tgcgaaatca ccctgcggga
     4801 acggctgcga taaattcacc gagcagtagg accggatgga cctgttcatt cgcaccgtcg
     4861 gaatcgcttg agcgacggtc aaacgcttgc tcttcctgcg ccgtattgcg gcggtttgac
     4921 taaaagccat tatccggggg cttcaccccg agccgacgcc tcgcgaaatc cggcgctcgc
     4981 gctcctgacg caacatcctt catccgtcag gcggcgttaa tagaagcgtc cagcttcatg
     5041 ccgcg
//
LOCUS       DATMPU010000121         3241 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_2570784,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000121 DATMPU010000000
VERSION     DATMPU010000121.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 3241)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3241)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3241
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2570784"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..149)
                     /locus_tag="VNY06_02790"
     CDS             complement(<1..149)
                     /locus_tag="VNY06_02790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012383221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N utilization substance protein B"
                     /protein_id="HWX13791.1"
                     /translation="MATPGQRSAARLAAVQALYEMEISGKGLNETKLEFESFWIGNEI
                     EGVQY"
     gene            complement(149..640)
                     /gene="ribH"
                     /locus_tag="VNY06_02795"
     CDS             complement(149..640)
                     /gene="ribH"
                     /locus_tag="VNY06_02795"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012589702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HWX13792.1"
                     /translation="MSLTRRSPPVLGGSFEGARFLIVEARFYEDIAAMLLEGAKTAFA
                     KAHAAFEIVIVPGALEIPLAMAIALDHAAATQHPFDGAVALGCVIRGETHHFEIVANE
                     SARALMQLALARRLAFGNGILTVDTEAQALHRADPVKGDKGGDAARAALSIFRFKQEI
                     RGH"
     gene            complement(769..1137)
                     /locus_tag="VNY06_02800"
     CDS             complement(769..1137)
                     /locus_tag="VNY06_02800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX13793.1"
                     /translation="MSGFGSFCGISSGLVGILIAALALGGCNKTTATAPVAPAVPQGP
                     ARPVWPSLPETAACTKELNHYQTVLDADVGTGNLNRSVYEEIEADLSRAASACADGKD
                     GEARAIIHSTKIKHGYRASA"
     gene            1825..2274
                     /locus_tag="VNY06_02805"
     CDS             1825..2274
                     /locus_tag="VNY06_02805"
                     /inference="COORDINATES: protein motif:HMM:NF014732.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzz/FepE/Etk N-terminal domain-containing
                     protein"
                     /protein_id="HWX13794.1"
                     /translation="MNRMLKVDKSLLSDEVGQKPAGPPAEGPRNFYLRVLRHQRPTVI
                     VIMVLVMAIALIYLFTATPTYVGTAYMVIDTHQLQLLQDPQQAQRGAINVDNGMVSTQ
                     IQLLKSQNVSRAVIAKLNLTEDNEFAGPPGFFLIGRLTSLFTPWCRH"
     gene            2271..>3241
                     /locus_tag="VNY06_02810"
     CDS             2271..>3241
                     /locus_tag="VNY06_02810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="GumC family protein"
                     /protein_id="HWX13795.1"
                     /translation="MTEEEEKSQLLRRVQAKFEDQRTITRVEQSYEMEIDAQSEDRNK
                     AAKIANAIAEAYIDDTLEAKYQATRRANVWLQDRLQELRAQVAAEQKAVVAFRQKNNI
                     SYVDTGGGKFYVDTPGKLVNEQQLSELNSQLILAQAATAQAKARYDRIQEVMKQEIPD
                     ASVGDALNNQVIIKLRGLYLDLAARAGIYSQKYGSNHLSVVALRSQMREQLLAIRDEM
                     GKIEQSAKSDYEIALAREQSLFASVKSAMSQSQLTNQTQIQLQELESKAQTSRTLHDN
                     FLQHYMETIQQQSFPITEARLVGPADAPLSKSYPKTWLILLLALVGGS"
BASE COUNT          761 a          910 c          894 g          676 t
ORIGIN      
        1 ttatactgga ccccttcgat ttcattgccg atccagaagg actcgaactc caacttcgtt
       61 tcattgaggc ctttgcctga gatctccatt tcataaagtg cctggactgc ggcaaggcgg
      121 gcggcggaac gctggccggg agtggccatc aatgccccct gatttcctgc ttgaagcgga
      181 aaatcgaaag cgcagcgcgg gcggcatcgc cgcctttgtc gcctttgacg gggtcggcgc
      241 gatgaagagc ctgggcctcg gtgtcgacgg tcaatatgcc attgccgaaa gcgagccttc
      301 gcgcaagtgc aagctgcatc agggcgcggg cgctttcgtt cgcaacgatc tcgaaatgat
      361 gagtctcgcc gcggatgacg cagccaagcg caacggcgcc gtcaaagggg tgctgcgtgg
      421 ccgcggcatg atcaagggcg atcgccatgg caagcgggat ttcgagcgca ccgggcacga
      481 tgacaatttc gaacgcggca tgcgccttcg caaaagcagt ttttgccccc tcaagcagca
      541 tcgcggcaat atcttcataa aagcgcgcct cgacaatgag gaagcgggcg ccctcgaaac
      601 ttcctcctaa aactggcgga gaccggcgcg tcaaagacat gcttggttac tcggagagag
      661 gcgcgcgcga gcctaagcga aacagcccgc cagaccattt tcgaataaat aggatggctt
      721 ggctcttagg ccgccaaaag gcaaacggct gccgccatct tgctgtgcct aggcagaagc
      781 acgatatcca tgcttgattt tcgtcgaatg aatgatcgcg cgcgcttccc cgtcttttcc
      841 atctgcgcaa gcacttgccg cacggctgag atcagcctca atttcttcgt aaacagaccg
      901 gttgagattt cccgtaccaa catcggcatc cagcacggtt tgatagtgat tgagttcttt
      961 cgtgcaggcc gcggtctcgg gcaggctcgg ccagaccggc cgggccgggc cctggggaac
     1021 ggctggcgct acgggcgcgg tcgccgtcgt tttattgcag ccgccaagag caagtgcggc
     1081 aatgagaatc ccgaccaacc cggacgagat gccgcaaaat gagccaaatc cagacattct
     1141 ccgcattgca aactccgggt agcgcgccga gttttaggat ctccagccgc catcgtccct
     1201 acatatttgg ccgcctataa tcaataatta ccgctcaaag atccaccgtt cgccggccta
     1261 tttccttgag gctcatacca aagtcggccg cgagcgccgc gagacacccc agtccgagtc
     1321 tcggctcaac tccacgccgc caacatgatc tttgctatag gaagatttca ttgccaatga
     1381 ttggtctcag cgtcgaggcg gctcggcagg tcggtttttg cggcgcacaa ttacctagaa
     1441 ttgacgcata aactaatgta tgatgttcta ggtcagcaaa cagcgccgtt gggcgcgcgg
     1501 ctttcgcccc tttcaggaat acctttcgaa acgggttcaa tttttgcgtc aatcatcatt
     1561 taacacccac tggtcgattg cgttggaagg accgtgcgag ttcgcaagaa acggggtatc
     1621 ccgattcatc cttcagaggg gcgcacggct gtgcttagaa tgcacgattg cgagagcaaa
     1681 cattatagcc caagcatgga tcatgcggga ccgaacgggg gcggagaatc gatcgtgcga
     1741 ccattttgcg ctctgaagca aaggtgattt ttcttttggc tcatttgcga agacaggcgg
     1801 cagcttatta gctgtacgag cggcatgaat aggatgctaa aggtcgacaa aagtcttctt
     1861 tcggatgagg tggggcagaa gcctgcgggc ccgccggccg aagggccgcg caatttctat
     1921 cttcgcgttt taagacatca gcggccgaca gtgatcgtaa tcatggtttt ggtcatggcg
     1981 atcgcgctta tttacctatt taccgcgacc ccgacttatg tcggcaccgc ctacatggtg
     2041 attgacacgc atcaactgca acttctgcaa gatccgcagc aggcgcaacg tggcgccatc
     2101 aatgtcgata atgggatggt gtcaacccag attcaacttt tgaaatcaca aaatgtcagc
     2161 cgggccgtaa ttgcgaagct aaatcttacc gaggacaacg aattcgctgg ccctcctgga
     2221 ttctttctca tcggtcgctt gacgtcccta ttcacgcctt ggtgccgcca ttgaccgagg
     2281 aagaggagaa atcgcagctc ctgcggagag ttcaggccaa attcgaggac cagcggacca
     2341 taacccgggt cgagcaaagc tatgagatgg agatcgacgc gcaatcggag gaccgcaaca
     2401 aagctgcgaa aattgccaat gcgatcgccg aggcttacat cgatgacacg ctggaagcta
     2461 aataccaagc gacacggcgg gccaatgttt ggctccagga tcgcttgcag gagcttcgtg
     2521 cacaagtcgc cgcagagcag aaggctgtcg tagcttttcg ccagaagaat aacatctcct
     2581 acgtcgatac cgggggcggc aaattctacg tcgacacccc tggaaagttg gtgaacgagc
     2641 agcaactctc cgagctcaac agccagctta ttcttgccca agcggcaacc gcgcaggcga
     2701 aggcccgcta cgaccgcatt caagaggtga tgaagcaaga gattccggat gcgtccgtcg
     2761 gcgatgctct caacaaccaa gtgatcatca aactgcgggg gctatatctc gatttggccg
     2821 ctcgcgctgg aatttactca cagaaatacg gttccaacca tttgtccgtc gtcgcgctac
     2881 gctcgcagat gcgtgaacag ctgctcgcca tccgggatga aatggggaag atcgaacaaa
     2941 gcgcaaagag cgattacgag atcgcattgg cgcgggaaca gagtttgttt gccagcgtca
     3001 aaagcgcgat gtcgcaatcg cagttgacca accaaaccca aattcagttg caggaattgg
     3061 aaagcaaggc ccagacctca aggacgctcc atgacaattt cctccagcac tatatggaga
     3121 caatccagca acaatccttc cccatcaccg aagcccgtct tgtgggaccg gccgatgccc
     3181 cgttgagcaa gagttatccg aagacttggc tcatcctgct gctcgctttg gtgggcggct
     3241 c
//
LOCUS       DATMPU010000122         3488 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_2572695,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000122 DATMPU010000000
VERSION     DATMPU010000122.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 3488)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3488)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3488
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2572695"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..436)
                     /locus_tag="VNY06_02815"
     CDS             complement(<1..436)
                     /locus_tag="VNY06_02815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009489630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate binding protein"
                     /protein_id="HWX13796.1"
                     /translation="MKRRELMLLVGGALTATRTLRAQQEVMRVIGSLSPGSPSWAQAP
                     FWNALRQGLSESGYVEGQNVAVEYRWAEHRYDQLPSLAADLVGRNVDVIVTSGTPGIL
                     AAKSATSTIPIVFTGGGDLVATGVIASMARPGGNLTGVGTMNP"
     gene            504..734
                     /locus_tag="VNY06_02820"
     CDS             504..734
                     /locus_tag="VNY06_02820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018269317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HWX13797.1"
                     /translation="MPKGPQGQKRPADTVANAIRVAKILTGEVEEDMGDSGKDKAAQA
                     LGKKGGVARAKKLSPEDRAAIARKAAAARWKG"
     gene            complement(664..1704)
                     /locus_tag="VNY06_02825"
     CDS             complement(664..1704)
                     /locus_tag="VNY06_02825"
                     /inference="COORDINATES: protein motif:HMM:NF022021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P63C domain-containing protein"
                     /protein_id="HWX13798.1"
                     /translation="MDENGLRGQAGGRARAEILPPERKQEIARKAAQARWLAATHRGN
                     FKKDFGLDIDCYVLNDALKTAVISQRGMGQAIGFSKRGSRLPVFVNSKTMDGYIGRDL
                     RAKIEKPLVFQASAAAAGNSVSGRAYGYDAAILIDLCNSILTAKADGKLKGGRYNRMT
                     EQAQTIVSASAKSGIQRLVYALAGYNPSSEEVIEAFKLYVQEEARKYEPEFPNELYMQ
                     WHRLYNLAVPDRGKPWQFRHLTVRHVYYPLAKSSGKILDLLRALKAQDGDQSKKLFQF
                     LNKIGARALRIQLGRILEMAESSPNRTEYEKKVIDRFGGQRELDLVLPIPSIAPPLPS
                     AQLRLDLPGIAS"
     gene            1840..2748
                     /locus_tag="VNY06_02830"
     CDS             1840..2748
                     /locus_tag="VNY06_02830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_040609789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HWX13799.1"
                     /translation="MNKLPLAKRVQILSMLCEGSSMRSISRVAEVSINTVYKLLAEAG
                     PVCTRFHDATVHGVASQRIQCDEAWAFCYAKQKNVAGAKAAPEGAGDVWTWTALDAES
                     KLILSWAVGGRDAGYALALMDNLRSRITTRVQLTTDGHRAYLEAVEEAFGADIDYGML
                     VKLYGAETGGQGHERKYSPSECIGARKQTITGNPDPKHVSTSYTERHNLTMRMSMRRF
                     TRLTNAFSKKVDHHCDALALYFVFYNFVRRHQTLRVSPAMAAGISDRLWSMEDIVALI
                     DTAEGEPKKRGSYKPRQPKSDAEISN"
     gene            3348..>3488
                     /locus_tag="VNY06_02835"
     CDS             3348..>3488
                     /locus_tag="VNY06_02835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007676863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HWX13800.1"
                     /translation="MTDFPRSLIEFQQRFGDEAACAQYLIATRWPDGFVCPGCGGGKA
                     WRL"
BASE COUNT          717 a          986 c         1011 g          774 t
ORIGIN      
        1 cggggttcat agtgccgacg cctgtgagat tgccgcccgg ccgggccatg ctggcgatga
       61 cgccggttgc gactagatcg ccgccccccg tgaagacgat tgggatggtc gaagtagcgc
      121 ttttcgctgc taatattcca ggggttccgc tcgtcacgat aacatccaca tttcgaccga
      181 caagatcagc ggctagtgag ggcagctgat catagcgatg ctccgcccag cggtattcga
      241 ccgccacgtt ttgcccctca acgtagccac tttcgctcaa tccctggcgt aatgcgttcc
      301 aaaaaggcgc ctgtgcccat gagggcgaac cagggctgag ggaaccgatc acccgcatca
      361 cctcctgctg tgcgcggagg gtgcgcgtcg ccgtcagcgc tccacctacg agaagcatca
      421 gctcgcggcg tttcatttaa tcgcctcgct tttcgctctc gcgccgttat atgcttagcg
      481 acaggcaatg atcgagaggg acgatgccga agggaccgca aggccagaaa cgacccgccg
      541 ataccgtcgc caacgcgatc cgcgtcgcca agattctgac cggagaggtc gaggaggaca
      601 tgggcgattc gggcaaggac aaggctgctc aggcgctcgg gaagaaggga ggcgtcgcgc
      661 gcgctaagaa gctatccccg gaagatcgag ccgcaattgc gcggaaggca gcggcggcgc
      721 gatggaaggg atagggagca ctagatccag ctctctctgt cctccaaacc gatcaataac
      781 tttcttctcg tactctgttc tgtttggcga tgattccgcc atttcgagta ttcgtccgag
      841 ttgtatccgg agtgcccgtg caccaatctt atttaagaat tggaataact tcttgctttg
      901 atccccatct tgcgctttaa gcgctcttag gagatcaagg attttcccgc tactttttgc
      961 cagaggataa tagacatggc gaacagtcaa gtgcctaaac tgccagggtt tcccgcgatc
     1021 aggcactgcg agattataaa gacggtgcca ttgcatgtaa agttcattcg gaaactccgg
     1081 ctcgtatttt ctcgcttctt cttgaacgta gagcttaaac gcttcgataa cttcttctga
     1141 cgatgggttg taaccagcca atgcatagac caggcgctgt atgcctgatt tcgccgatgc
     1201 gctaacgatg gtttgagcct gttcggtcat ccggttatag cggccgccct tcagctttcc
     1261 gtcggctttt gccgtcaaga tcgagttgca gaggtcgatc aagatagcgg cgtcgtagcc
     1321 gtaggcgcgt cctgaaaccg agttccccgc ggccgcggcc gatgcttgaa aaacaagagg
     1381 tttttcgatt tttgccctta agtcgcggcc tatgtagccg tccatggttt tgctgttcac
     1441 aaagaccggg agacgcgatc cgcgcttgct aaagccgatc gcttgaccca ttccgcgttg
     1501 gctgatcacg gccgttttca gggcgtcgtt cagcacatag caatctatat cgaggccaaa
     1561 gtcttttttg aaattgcctc gatgcgtcgc ggcgagccat cgtgcttgcg ccgccttccg
     1621 tgcaatttcc tgttttctct cgggcggcaa tatttcagcg cgcgccctgc cgcctgcctg
     1681 gccgcgcaac ccgttttcgt ccatttgcac gcatcctcca tttctggaac atgcttgcat
     1741 gatacgccgg actagctcac cattgcaagc atgaaagcgg aggcacttat gcttgcgaat
     1801 ttccttgacg ccaagcaaaa acgttcagat aataatggca tgaacaagct gcccctcgcc
     1861 aagcgcgttc agatcctttc catgctctgc gaaggctcat cgatgcgctc gatcagccgg
     1921 gttgctgaag tatcaatcaa taccgtctac aagctgttgg ctgaggctgg cccggtctgt
     1981 acgcggttcc atgatgcgac cgtgcacgga gtggcgagtc agcgcatcca atgcgatgag
     2041 gcttgggcat tttgctacgc caagcagaag aacgttgctg gcgctaaagc cgccccggag
     2101 ggcgctggcg acgtttggac ctggaccgca ttggacgccg aatcgaagct aatcctgtcc
     2161 tgggcagtgg gcgggcgcga tgcgggctat gcgctggcgc tcatggataa tctacgaagc
     2221 cggatcacaa cgcgggtgca gctcactacg gatgggcatc gcgcctatct ggaagccgtt
     2281 gaagaagcct ttggcgcgga tatcgattac ggaatgctgg tcaagctgta cggcgcggag
     2341 actggcgggc aagggcacga acgcaaatac agcccctcag aatgcattgg cgcgcgcaaa
     2401 caaactatca ccgggaaccc ggacccgaag cacgtcagca cgagctacac ggagcggcac
     2461 aatttaacta tgcgcatgag catgcgtcgg ttcacgcgct tgaccaatgc gttcagcaag
     2521 aaggtcgatc accattgcga cgcgctggcg ctctatttcg tgttctacaa cttcgttcgc
     2581 cgacatcaga cgttgcgcgt ctcgccggca atggcggccg gcatttccga tcggctatgg
     2641 tcgatggagg atatcgttgc gcttatcgat acggctgagg gtgaaccgaa gaagcgcggc
     2701 tcatacaagc cgcgccaacc gaaatcggac gcggagattt caaattgagt cactacccat
     2761 cttcgggacg tcttgacgag atgcgcgggt cgggagcggc ggtaggttta gttgccttgc
     2821 cgattgcggg tctggttctc ctcattctta agtttttggc atcaagctgg ttcagcaggc
     2881 ggcaaatgcc gagcgcgata ccgatgcttt gatgaccgaa agcggttagc gccgctcgcc
     2941 ggtcacgacc atatccgtgt cgttgttatg tgcttggcaa catcagtctg ggttgggatg
     3001 atcctttatt gcgtggctcc cgcaatgaga acagacgagc cgttacgcga ctcgggaaca
     3061 ggagtctcgg ggtcgtatcg taggccattt cggcgggatc gagctcaacc tacggcaatc
     3121 ccggcacccg gcgaagccgt gtggctcccc cgaaggggag gggcaccaca cgatcaggcg
     3181 aacatgggca gccgcagcat tcccgagcgt catcggcggg gccgggctcc gacctcattc
     3241 acgagttaag ccttatccct ctgcgtccgg cggcacaaaa tgttgtagct gcgagggcag
     3301 gttcgtgata gaacagataa tgaacataac tgccctcggc agctccaatg accgactttc
     3361 ctcgctcctt gatcgagttc caacagcgct tcggcgatga ggccgcctgc gctcagtatc
     3421 ttatcgcgac gcgttggccc gacgggtttg tctgcccggg atgcggtggt ggtaaagcct
     3481 ggcggctg
//
LOCUS       DATMPU010000123         2931 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_95944, whole
            genome shotgun sequence.
ACCESSION   DATMPU010000123 DATMPU010000000
VERSION     DATMPU010000123.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 2931)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2931)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2931
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_95944"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(113..271)
                     /locus_tag="VNY06_02840"
                     /pseudo
     CDS             complement(113..271)
                     /locus_tag="VNY06_02840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011044137.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(548..2264)
                     /locus_tag="VNY06_02845"
                     /pseudo
     CDS             complement(548..2264)
                     /locus_tag="VNY06_02845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018329524.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
     gene            2640..>2931
                     /locus_tag="VNY06_02850"
     CDS             2640..>2931
                     /locus_tag="VNY06_02850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HWX13801.1"
                     /translation="MSQQQRGQRQQPDGEPTHINQLQATLNVVPAHTWYTTSSGGLTF
                     VNKRIADYLGLPNDHPLRFGIDIGAPWDAHIPLLHPDDHEEARKVWSTCLRTG"
BASE COUNT          614 a          852 c          823 g          642 t
ORIGIN      
        1 ccggtgcaaa gccaaacgcc aaaggaaccc tattgtctgc aatgggtctt ggtcgtgtaa
       61 aagcggccct tagggacgtc ggtcgtggta gcccaggagc ctcgaggtcc caggccacga
      121 tggccgcgat cagcggcttg atcctgacga tgttcatgac ccgtgtcaga ttataggcca
      181 ggactgagag ggccatttcg gcggctacct ttgggagcgc tttggtcagg aagtgtgtcg
      241 cgcccatgcg agcctttatc gtgccgaacg gcatggccaa gaggttgttg tcgaggcgcg
      301 tcgtggcgaa cagtggctta gcttaccgat gacgcgctac cgcgattcat tcgctcactt
      361 ggtagcttgc caactttcat cagctgggct cgatggtgtt ccgatcatgg caatcgacag
      421 catgactgcc gagaagcgcc taaccgaagc gggtattggc attggacttt tgccgggcca
      481 aagccggaag ttcgcgggcc ttattcgacc acctcgtcgg cgcggacgag cagcgtcgcc
      541 gtcagggtca ttccggcagc cctgctaagc gcaggccctc tgtgtatctg gcgaaatctt
      601 cagagctgaa tggaggcaaa acgtcagcga gattagatat ccgtaacgtt ggatcgattt
      661 ggcggatgcg tgacattgct ttttgggcct gctcaatacg acctgcaaat gcgtgactag
      721 cagcaaaggt gcgcagcgcg ccaggatgat ccggctgctc tcgcagcacc atttccgccc
      781 acaaagccgc gtcatcgttg cgtccggcga aaaagtgagc catcgccatg tagaagtgcg
      841 acctgtgcat cgatggatcg actgggttaa gtcgcattgc gtgcgcaaca ttttcgatcg
      901 cattttcagg ccgcccccgg caaatgtcca tccaacctgt atatgcccaa acgaatgcga
      961 ggtttggatt gagtgtgcgc gcgcgatcaa cgtaagttgc gcctcctgcc aggtcacgag
     1021 caacataagc aagaatgtac cctgcagttc cgagcacgac cgcgtcgtcg ttactcaacg
     1081 cgaccgcctg tcggaccagc cgggtcgcct cggcgatttc tgttgcgcgg tcaatcatcc
     1141 aaccatttac tctacgcaat gcatagcatc gagctgccat ggcgtagggc gaagcgaaag
     1201 ccggatcgag cgcgatagcc ttcaagtaca aacgcaatgc ctcttgattg gcatccttcg
     1261 ttgcaccctg gtaggccgcc atgccgcgca gataataatc gtacgcatct aggctttcag
     1321 ttggcttccg ccttgcgcgc gcaatctctg cttgctcaag cttgggagcg atcaatccga
     1381 caacgctggc ggtgacttcg tcttgcaggt caaaaacgtc ctcaaggcgg ccgttgaagt
     1441 gatcagccca aagatgcgtg ccagtcgcag cttcgattag gctgcccggt gatgcgaacg
     1501 cgattgcccg cttttcttac gctaccttca aggacgtagc ggacccctag ttcgcgccca
     1561 acctgcttca cgtcgacagc cttacccttg taggtgaagg tcgaattgcg cgcgatcacg
     1621 aacagcaatt tgaagcgaga tagcgcggtg atgatatcct ccaccattcc atcagcaaaa
     1681 tactcctgct gggggtctcg actcatgttc tggaaaggca gcaccgcaat ggagggcttg
     1741 tcgggaagcg gaagcggctg gttggggacc ggagaagctt ttgcggccgg tgtggaagcc
     1801 agcttgttgc ccgactggac gctccacgcg cgcatgggtt cggcgatgtt cttcaaggcc
     1861 tgaaggccca tgtcatcgta gatgatgtcg atctttcctc ggacttggcg ataggcatcg
     1921 tcagaaatgc aaatgccacc cggcttcgcg atcccctcta gacgggcggc gatgttaacg
     1981 cagtcgccga aaatatcgtc ttcgtcgatg ataatgtcgc caacatgaat gccgatacga
     2041 aactcgatcc gcctatcttg aggcgtgttg gcattttgtt ttgccatgcc gcgctgaact
     2101 tctacggcgc atcgcgctgc gtcgaccgcg cttgcaaact cgaccagcat gccgtcgcca
     2161 gtcgttttga ctattcgtcc gcgatgctcc gcgatcttgg ggtcaaccaa cccctttctg
     2221 aaggctttaa gccgggccag agtaccctct tcgtcgccgc ccataagacg gctataaccg
     2281 gcaatatccg ctgcgagaat ggctgccagt cgccgatcaa cccgattgcc gctcaagtgg
     2341 gacccctcaa gaccggaaaa tcgctctctc cgatcaaacc acacagaagt gttatcccgc
     2401 tagggcaaat gtccgagctg gatcatttgc gtcgattggc ctcatgccag ccatttccga
     2461 tctactgcca atagccgact tccggtcgat gtggggaccc atagcggaca ttgttctgca
     2521 gcttcgggcc cggcggtcgt catcccgcta cgcaaaacgc gtgcatgctc ggtttgatca
     2581 ccaagtcgaa tttcggtagg gtcatacgtc tgggacgtga ctctgggtgg aattggcgca
     2641 tgagtcagca acagcgcggg caacgccaac agccggatgg cgaacccact catatcaatc
     2701 agctgcaggc cactctaaac gtggttccgg cgcatacttg gtacacgact tcctccggcg
     2761 gactcacgtt cgtcaataaa cgaattgcgg attaccttgg tcttccaaat gatcatccgc
     2821 tacgtttcgg catcgacatt ggcgcaccgt gggacgccca catcccgttg ctgcatccgg
     2881 acgaccacga agaagcgcgc aaagtctggt caacctgcct gcgcacgggc g
//
LOCUS       DATMPU010000124         6319 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_2573712,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000124 DATMPU010000000
VERSION     DATMPU010000124.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 6319)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6319)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6319
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2573712"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1088
                     /locus_tag="VNY06_02855"
     CDS             <1..1088
                     /locus_tag="VNY06_02855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012384335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HWX13802.1"
                     /translation="EILADHHIVFIGPKPEHIRLMGDKIEAKATALRLGIPCVPGSTG
                     AVTSDREAASISEGLGFPVLVKAVAGGGGRGMKIARSASDLPVAIETARIEATAAFGD
                     DSVYVEKFLEKPRHIEIQILGDGYGKAIHFGERDCSLQRRHQKLWEESPSPALNVSQR
                     LEIGEICATAMRELGYSGLGTIEFLYEGGKFYFIEMNTRIQVEHPVTEMVTGIDLVHE
                     QIRLAAGSPLTLTQNQIVLQGHAIECRINAEHAASFRPSPGKIAYYHPPGGLGVRVDS
                     AVYAGYNIPPNYDSLVGKLIVHGRNRNEALMRLRRALDEFIVDGIDTTIPLFQTLVRN
                     ADIQNGLYDIHWLEKFLALGGMDAAGM"
     gene            complement(1163..2464)
                     /locus_tag="VNY06_02860"
     CDS             complement(1163..2464)
                     /locus_tag="VNY06_02860"
                     /inference="COORDINATES: protein motif:HMM:NF016830.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate porin"
                     /protein_id="HWX13803.1"
                     /translation="MLPPELQPTIASSVPALAEFKNGLYDLGYNLLFSYFADPWANPT
                     GGVRQGAAYEGLLYMVLDADLAKIAGLDGLNFRASAYQIHGRQLSKDNLFNLAPIDGI
                     EARPTTRLFELWIEKKFSDFARIRIGQLSADNQFFISEFGNSLYVNSTFGWPTIFHAD
                     LPGTGPNYPLATPGVRIKVTPNDQLAFLVGLYNGDPAGSGFTGLEEIKDPAGINFRLK
                     DPPLLMAEVQYRYNQDKAASGLAGTIRLGGLYHFGKFNDQLFDVDGQSLAAPASNGIK
                     KTYRGNYSLYGVIDQMLWRLPGDDPKKGVGAFARVAGTPMDRNLIDFYAEAGINFMGL
                     WDKRPDDNFGIASSFTHLSPNVRELDAETALFTKTPLPLRNYELVFELTYQAQVISGW
                     TVQPDFQYIFHPGGGTIDPVNPFVGRIPDAAVFGLRTQISF"
     gene            complement(2610..2683)
                     /locus_tag="VNY06_02865"
     tRNA            complement(2610..2683)
                     /locus_tag="VNY06_02865"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2649..2651),aa:Gly,seq:tcc)
     gene            2800..3015
                     /locus_tag="VNY06_02870"
     CDS             2800..3015
                     /locus_tag="VNY06_02870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX13804.1"
                     /translation="MRSVNAAGHDFRVALRSSPRESELRGSNTHRKKSASLLRVIESG
                     SVQQAVFMDEDFIYSKDRLVFFKIYLN"
     gene            3254..4957
                     /locus_tag="VNY06_02875"
     CDS             3254..4957
                     /locus_tag="VNY06_02875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015664745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX13805.1"
                     /translation="MTSALAMASFLAASVSAEAVGSGAQPSYEQLLEKLDRMEARIRS
                     LEGELHHKAERDANQGSKAHQAKTTNRSPAKGSKEATNAAEQTAGQQSAALEPPAPAK
                     PPPGANLHLAPAPGYLGAAETAALETPPPASTDLFGVAPAPVHGLRIGAYGEFAFGAR
                     QNPDAFGQWQTGFDTVRLVLTPTYQFNDNIIFNSEIEFEHGGVAFDADDKLHGTAEIE
                     QAYVDFRISPYFNIRAPGIDLVPIDWLNLYHEPTNFYSVRRPELDNGLIPSTWKAPAA
                     SIWGQIVEGLNYQFQVSQALEDFGSDFSLRTDANGVPLFPLGYAPGIDGIDALGFSQA
                     PLGDFRQLSNYLGYTFRLSYAPTFLPGFDGGSSVYFTPSTTPRGAYGDTGILLGRSSL
                     TIFETDFRYHVPDTGLEFRGEFADVIFGNPANLRANNDTDPFDNVGKSMFGGSAEVAY
                     HVPLGPIFGPILGSNWEAVPFYRYTYQNFQTGGFAGTDFNFPTGLGVMQFHDVGFAVY
                     PTPELVLKLNYTKALNLAPGGAQSDSVLGGVGFFFDVQTARTCSRRDAGSTFRPTNIG
                     V"
     gene            4965..5426
                     /locus_tag="VNY06_02880"
     CDS             4965..5426
                     /locus_tag="VNY06_02880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006614956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HWX13806.1"
                     /translation="MVGFVGAPAAAVAITLPAYAYQYSSVGAAQRQAFPGARFEEIQP
                     QRVWKALAGDRLIRYFFVDHVIGKHLYIDYSVALGADGRVRRVEILTYRESYGFEVAN
                     AGWLAQFAGKTSGSALEINSDIRNISGATLSSRHVTEGVKRILAYYATHLR"
     gene            5407..>6319
                     /locus_tag="VNY06_02885"
     CDS             5407..>6319
                     /locus_tag="VNY06_02885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008962935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HWX13807.1"
                     /translation="MPPTCDNIRRLRPLLGTFVEVAVAGAAVADMEAAVEAAYAAIRT
                     VHRLMSFHDEESDVSRLNHGAFEAATGVHHWTFQVLEAALDFHRRSNGMFDIRIAPAL
                     QRFGLLPYHAGDLAHDAPLTLGQCGIELLADDRVRFDDRATRIDLGGIAKGFAVDRAI
                     DVLRDLGMPSGLVNAGGDLAVFGPRGLMVTVRDPGRPSRVLCHVELRDAAIASSGRQF
                     DPLQSLDAWGPAVIDPASGEPVCALIGATVRAPSCLLADALTKVVMAGGPYAAPVLRQ
                     FDADALFVSAEGEVYITPEWRDAAVLAA"
BASE COUNT         1358 a         1809 c         1789 g         1363 t
ORIGIN      
        1 ccgagattct tgccgaccat cacattgtct tcattggccc caagcccgag catatccggc
       61 ttatgggcga caagatcgag gcgaaagcaa cggcgctccg gctcggcatt ccctgtgtcc
      121 ctggctcgac cggcgcggtc accagcgata gggaagctgc ttctatctct gagggactgg
      181 gttttcccgt gctcgtaaag gcggtggcgg gcgggggtgg acgcggcatg aaaatcgccc
      241 gcagcgccag tgatcttccg gttgcaatcg aaacggcaag gatcgaagct acggcggcct
      301 tcggcgatga ctcggtgtat gtcgaaaaat tcctcgaaaa gccgcgccac atcgaaatac
      361 agattctcgg cgatggttac ggcaaggcga ttcacttcgg ggaacgcgat tgctccttac
      421 aacgccggca ccagaaactc tgggaagaga gcccatctcc tgcgctcaac gtatcccagc
      481 gcctggaaat tggcgagatc tgtgcaactg caatgcgtga attgggctat agcggactag
      541 gcacaatcga gtttctctac gaaggcggca aattctattt tattgaaatg aatacaagaa
      601 ttcaggtcga gcatccagtt accgaaatgg taactggtat tgatctcgtt catgagcaaa
      661 tccgacttgc cgcgggatct cccctgacgt taacccagaa ccagatcgtc cttcaggggc
      721 atgctatcga atgccggatc aatgcggagc atgcggcaag cttccgccca tcgcctggaa
      781 aaatcgccta ttatcacccg ccaggcggcc ttggcgtgcg cgtcgatagc gcggtctacg
      841 ccggttataa cattccgccc aactatgact cgcttgttgg caagctcatt gtacatgggc
      901 gaaaccgcaa tgaagcactc atgcgccttc gtcgcgcgct cgatgagttc attgtcgacg
      961 gaatcgatac gacgattccg ttgttccaaa cgctcgtgcg caatgccgac atccaaaatg
     1021 gactctacga catccattgg ctggaaaaat tccttgcctt gggcggaatg gacgcggcgg
     1081 gcatgtaagg ctcagccggg cgcgacgcgg ccggaataaa gcacgtcgtg gttccccaaa
     1141 tttctctgcc tcacaatagc agttagaaac tgatctgcgt gcgcaaacca aaaacagcgg
     1201 cgtcgggaat gcggcctaca aaaggattga cgggatcaat cgtgccgcca ccggggtgga
     1261 agatatattg gaaatcgggc tgcacggtcc agccggaaat gacttgcgcc tggtaggtca
     1321 gttcgaatac caattcataa ttgcgcaatg gcaaaggggt tttcgtaaac aatgccgtct
     1381 cggcatcgag ctcgcggaca ttgggagaga gatgcgtaaa ggatgaagca atcccaaaat
     1441 tgtcatccgg ccgcttgtcc caaagcccca taaaattgat gcctgcctcg gcatagaagt
     1501 cgatcaaatt gcgatccatg ggagttcctg caacacgcgc aaatgcgccg acgcccttct
     1561 tcggatcgtc gcccggcagg cgccagagca tctggtcgat cacgccgtaa agggaataat
     1621 ttccccggta agtctttttg atgccgttgc tcgctggggc ggcgagcgac tgaccatcga
     1681 catcaaaaag ctgatcgttg aacttgccga agtgatacag tcctccgagc ctgattgtcc
     1741 cggcgaggcc tgaggccgcc ttgtcttgat tgtaccggta ttgcacctcg gccattaaca
     1801 atggcggatc cttgaggcga aagtttattc ccgccggatc tttgatctct tccaggcccg
     1861 tgaacccaga ccccgctgga tcgccattgt atagaccgac caaaaaagcg agctgatcgt
     1921 tcggcgtcac tttgatccga acaccgggtg tcgccaaagg atagtttgga cccgtgcctg
     1981 gcaggtctgc atggaatatt gtcggccacc cgaacgtgct gttgacatac aaactgttac
     2041 cgaattcgct gatgaagaat tgattgtcgg ctgagagctg accgatgcgg attcgggcga
     2101 agtcactaaa tttcttttcg atccaaagct caaacagccg tgtcgtcggc cgcgcctcga
     2161 tgccgtcgat gggtgcgaga ttgaaaaggt tatctttgga cagctgacgg ccatgtattt
     2221 gataagcgct ggcgcgaaaa ttgagaccgt ccaaccctgc aatcttcgca aggtccgcat
     2281 cgaggaccat gtaaagaagg ccttcatagg ccgcgccttg ccggacaccg cccgtcggat
     2341 tggcccaggg atccgcaaag tagctgaaca gcaaattgta accgagatcg tagaggccgt
     2401 tcttgaactc agcgagcgcc ggaacgcttg aggcgatcgt cggctgcagc tctggcggca
     2461 gcactacatt tctccgcccc ggtataggaa tggtggcgtc ctgcgcctgc cccaatttgc
     2521 tacttgctgc ggcgcatccg acacccagaa gcacaacaaa tataagcctg cgccacatgt
     2581 gtgtccctgc caaaaatggg cgcggaaatt ggagcgggtg aagggaatcg aaccctcgta
     2641 ttcagcttgg aaggctgctg ctctaccatt gagctacacc cgcgtcagtt ttatgtcact
     2701 tgcctcgcgt caatgaccgt cttccacggg acccggtcat cctaaggccg agtgagacat
     2761 tagcggcact tcgtgtgcgg ccgaagctat aaggcgagct tgcgctctgt caacgcggct
     2821 ggacatgatt tccgggtcgc gcttcgttca tcgcctcgag aaagtgagtt gcgcggaagc
     2881 aacacccacc gcaaaaaaag cgccagcctg ctgagggtaa ttgagtcagg ttcggtccaa
     2941 caggcagttt tcatggatga agattttatc tattctaaag accgcttggt gttctttaaa
     3001 atctatttaa actagcattt gagattgaca ttcgatgttt gaaataaaat agtatagaag
     3061 gaagctagat tagaatgagt actatctaca ttgatctggc gcgcaactga accacgaatc
     3121 tggccatgga aagagcgcgg atgcacctga gccaacgaaa catgaggcgg aaatgccgaa
     3181 ccactgcatc ccatgtactc cacttgcttg ccggactgat ttccatgacc aggctggaaa
     3241 gcgttattcc gcattgacct ccgcacttgc catggcttcc tttctggccg cctcggtcag
     3301 cgcggaagct gtggggtctg gcgcacaacc aagctatgaa caattgctcg aaaagctcga
     3361 tcggatggag gcgcgcatcc gttcgctcga aggcgagctg caccacaaag ctgagcgcga
     3421 tgccaatcag ggttccaagg cccatcaggc caagaccact aaccgcagtc cagcaaaagg
     3481 atcaaaggaa gcaacgaatg ccgcggaaca gactgctggc cagcaatccg cagcgctcga
     3541 acccccagcg cccgccaaac cgccgcctgg tgcaaacttg catctggcac cagcccccgg
     3601 gtaccttggc gcagcggaaa cagccgcctt agagacgccg ccgccagcca gcacagattt
     3661 attcggcgtt gcgcccgcgc ccgtccacgg cctcaggatc ggcgcctatg gcgaattcgc
     3721 tttcggggca cggcaaaacc ccgatgcgtt tggtcaatgg caaactggct tcgatacggt
     3781 ccggctggtt ctaacgccaa cctatcagtt caacgacaac atcatcttca attcggagat
     3841 cgagttcgag cacgggggcg ttgcgtttga tgccgacgac aagctgcatg ggacggccga
     3901 gatcgaacag gcctatgtcg attttaggat ttctccgtat ttcaacattc gcgcgcccgg
     3961 catcgatttg gtgccgattg attggctcaa tctgtatcat gagccgacca atttctacag
     4021 cgttaggcgc ccggaactcg acaacggcct gataccgtcg acgtggaaag cccccgccgc
     4081 cagcatctgg gggcaaatcg tcgagggtct gaactatcag tttcaagtca gccaagctct
     4141 ggaggatttc ggcagcgatt tcagcctgcg cacagatgcc aacggcgtcc cgctttttcc
     4201 actcggatat gctccgggga tcgacggcat cgacgcgctt ggtttttcac aagcgccttt
     4261 gggcgacttc cggcagctca gcaattatct aggttacact tttcgcctga gctacgcgcc
     4321 aacgttcctg cctggatttg acggcggctc gagcgtttac ttcaccccaa gcacgacccc
     4381 acgtggcgcc tatggcgata ccggcatctt gcttggccgc tccagcctca caattttcga
     4441 gaccgatttc cgttatcatg tcccagacac gggtctcgaa ttccgcggcg aattcgccga
     4501 cgtcatcttt ggcaatcctg cgaatttgcg agctaacaac gacaccgatc ccttcgacaa
     4561 tgtcggcaaa agcatgtttg gcggatccgc agaggtcgct tatcacgttc cgcttggccc
     4621 aatctttggt ccgatcctcg gtagcaattg ggaagcggtt ccgttctatc gctacaccta
     4681 tcagaacttt cagacgggcg gcttcgcggg aaccgatttc aacttcccca caggcttggg
     4741 cgtgatgcaa ttccacgatg tcggcttcgc cgtctatccg acaccggaac tggtgttgaa
     4801 gctcaactac acgaaggcac tgaatttggc tccgggcggc gctcaatccg acagcgtttt
     4861 gggaggggtc ggtttcttct ttgacgtgca gacggcaaga acgtgctccc ggcgcgatgc
     4921 tggtagcacg ttccggccaa cgaatatcgg agtctagcgc atggatggtt gggttcgttg
     4981 gagcgccggc ggcggcggtt gcgataaccc tgcccgctta cgcctatcaa tattccagcg
     5041 tgggggccgc gcaaagacag gcctttccgg gagcgcgttt cgaggaaatc cagccccaac
     5101 gggtgtggaa ggccctcgcc ggcgaccggc tcatccgtta ttttttcgtc gaccacgtta
     5161 tcggcaagca tctctacatc gattattcgg tcgctctcgg cgcggatggc cgggtccgtc
     5221 gggtggaaat cctgacctac cgggaatcct acggctttga ggtggcgaat gcgggctggc
     5281 ttgcgcaatt cgccggaaag acgagcggca gcgcgctcga aatcaattct gacattcgca
     5341 atatctcggg cgcgaccttg tcgtcgcggc atgtgaccga aggtgtaaaa aggatattgg
     5401 cctattatgc cacccacttg cgataatatt cgccggttgc ggccgttgct tggcaccttc
     5461 gtcgaggtcg cggttgcggg cgccgccgtc gcggacatgg aggcggcggt agaagcggcg
     5521 tacgcggcaa tccgcaccgt ccatcgcttg atgagcttcc atgacgagga gagcgacgtg
     5581 agccgcctca accatggcgc gtttgaagcg gcgaccggcg tgcatcattg gacctttcag
     5641 gttttggaag ccgcgctcga cttccaccgg cgctcgaacg gcatgttcga cataagaata
     5701 gcccccgctt tgcaaagatt tggcttgttg ccgtatcacg cgggcgatct tgcccatgat
     5761 gcgccattga cgttaggcca atgcggaatc gaattgctcg cggacgatcg agtccggttt
     5821 gacgaccgcg ccacgaggat cgatctcggc gggatcgcca agggatttgc cgtcgatcgc
     5881 gcgatcgatg tcttgcggga tcttgggatg ccatccggcc tcgtcaacgc cggcggcgat
     5941 cttgccgtct ttgggccaag gggcctaatg gttactgttc gcgatccggg ccgcccaagc
     6001 cgggtcttgt gccacgtcga gttacgcgac gcggcgatcg cgtcgagcgg tcgccaattc
     6061 gatcccttgc aatctttgga tgcgtggggc cctgccgtca tcgaccccgc cagcggcgaa
     6121 ccggtttgcg cgctcatcgg agccacagtc agggcgccct cctgcctgct ggcagacgcg
     6181 ctgactaagg tggtcatggc cggcggccca tacgccgcgc ccgtgctccg ccaattcgac
     6241 gccgatgccc tctttgtgtc ggccgaaggg gaggtttata tcactccgga gtggcgcgat
     6301 gctgccgttc tggcggctt
//
LOCUS       DATMPU010000125         4138 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_2894736,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000125 DATMPU010000000
VERSION     DATMPU010000125.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 4138)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4138)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4138
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2894736"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..387)
                     /locus_tag="VNY06_02890"
     CDS             complement(<1..387)
                     /locus_tag="VNY06_02890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012590191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate ferredoxin oxidoreductase family
                     protein"
                     /protein_id="HWX13808.1"
                     /translation="MADAVRGPVDLPQDLRFTSLDDKYDLTKRRIFVSGPQAIVRLLL
                     MQKELDRRNGLNTAGFISGYRGSPLGGLDLAFLRAKRPLDAAGILFQPGLNEDLAATA
                     IWGTQQAEMRGEGKFDGVFSLWYGKGP"
     gene            complement(533..1660)
                     /gene="pqqE"
                     /locus_tag="VNY06_02895"
     CDS             complement(533..1660)
                     /gene="pqqE"
                     /locus_tag="VNY06_02895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013165882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0018149 - peptide cross-linking [Evidence
                     IEA];
                     GO_process: GO:0018189 - pyrroloquinoline quinone
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroloquinoline quinone biosynthesis protein
                     PqqE"
                     /protein_id="HWX13809.1"
                     /translation="MSAAPAKIDPPAGLLAELTHRCPLGCAYCSNPLELDTRENELDT
                     GVWKRVFSEAAALGVLHVHLSGGEPAARRDLVAIVAHCAKEGLYTNLITSGIGVTEER
                     IAELANCGLDHVQLSIQDSDAQSADRIAGYNGAFARKEMVAAWVTREGLPLTVNAVIH
                     RANVSRARKMVELAVRLGARRIEIAHVQYYGWAMLNRAALMPPLAEAEAVIAEVEALK
                     KSFCGTIVIDHVIPDYHARTPKACMGGWGKRILNVTPSGKALPCHAAETIPDLIFWNV
                     RDHSLEDTWLNAPAFNVFRGVEWMREPCRSCPRKHIDYGGCRCQAFALTGDAREADPV
                     CHLSPNHSKVQEIAAILAQGEGRDGKPTYHYRRMKSREPAQ"
     gene            complement(1657..1929)
                     /gene="pqqD"
                     /locus_tag="VNY06_02900"
     CDS             complement(1657..1929)
                     /gene="pqqD"
                     /locus_tag="VNY06_02900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020185124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_process: GO:0018189 - pyrroloquinoline quinone
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroloquinoline quinone biosynthesis peptide
                     chaperone PqqD"
                     /protein_id="HWX13810.1"
                     /translation="MAESDSIPRLPRGVRLKYDNTRAEWLLLAPERVIKADKIAVEIL
                     KRCDGTATLGAIVDDLAGQFSADRARVETDVRALLSELAAKRMVDL"
     gene            complement(1944..2702)
                     /gene="pqqC"
                     /locus_tag="VNY06_02905"
     CDS             complement(1944..2702)
                     /gene="pqqC"
                     /locus_tag="VNY06_02905"
                     /EC_number="1.3.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012386228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0018189 - pyrroloquinoline quinone
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroloquinoline-quinone synthase PqqC"
                     /protein_id="HWX13811.1"
                     /translation="MIEAGQPMPGEELEARLRQIGAERYHNLHRFHRRLHSGQCTYEE
                     VQAWALNRYYYQATIPIKDATILTRMPDPADRRAWRKRIEDHDGLREGDGGIERWLKL
                     TDSLGFSRDYVASTQGILPATRFAVDAYVDFCRTKSLVEAVASSLTELFSPTIISERV
                     AGMLGHYGFVSQETLAYFSQRPVQAKRDSDFALGFVKTHALTRSEQKAVLAALEFKCS
                     ILWAQLDALWSAYVDRAIPPGAWLPGTNAHGQAA"
     gene            complement(2699..3595)
                     /gene="pqqB"
                     /locus_tag="VNY06_02910"
     CDS             complement(2699..3595)
                     /gene="pqqB"
                     /locus_tag="VNY06_02910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012453680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0018189 - pyrroloquinoline quinone
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroloquinoline quinone biosynthesis protein
                     PqqB"
                     /protein_id="HWX13812.1"
                     /translation="MRIIVLGSAAGGGFPQWNCLCPGCRLAWNGDKRVKPRSQSSLAV
                     SADGERWLLLNASPDLRQQIIASPSLHPRGGARHSPICAVFVTNADVDHLAGLLTLRE
                     QQSFMLFGSEATLEQAKAGVFGVLNKDLVQTRPVACDRKVDTGLGITVTPFAVPGKVP
                     LYLEGEDVEIGGENEATAGVEISNGAASFFYVPGCAKINEGLKTRLAGARLIFFDGTT
                     YTDDEMIALGLSNKTAWRMGHVAMSGENGSLKLLAPCRIERKIYVHINNTNSVLIEDS
                     SEREAVLRAGWEVAYDGMEVCL"
     gene            complement(3871..>4138)
                     /locus_tag="VNY06_02915"
     CDS             complement(3871..>4138)
                     /locus_tag="VNY06_02915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013950276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HWX13813.1"
                     /translation="NGILPIKVPAEDLAKLMDDSRRGSNATLSVHLESQEIRGPVGGV
                     ISFDIDPFRKHCLLHGLDDIGLTLQKASSIEAFEAKVAAERVWL"
BASE COUNT          895 a         1254 c         1149 g          840 t
ORIGIN      
        1 aggcccctta ccgtaccaga gcgagaaaac gccatcgaat ttgccttccc cgcgcatctc
       61 agcctgttgc gtcccccaaa tggcggtcgc ggcgagatcc tcattgaggc ccggctgaaa
      121 caaaatgccc gctgcatcga gcggacgctt ggcgcgcaag aaagcgagat caagaccacc
      181 cagcggcgaa ccgcgatagc cagagataaa gcccgcggta ttcagcccat tgcggcgatc
      241 gagctccttt tgcatgagca ggagcctgac aatcgcctga ggcccggaga caaaaatccg
      301 cctcttggtc agatcatatt tatcgtcgag tgaggtgaat cggaggtcct gggggaggtc
      361 gactgggcct cttaccgcgt ccgccatcgc ttacgtctcc tgccggacgc ttgcgcccgg
      421 ccccgcgaag tctgccattg cgcagaggca aatataagcg ggcagaaccc tttttccaag
      481 gtttcaccgt cttttccacc ggatgaacgc atgcttgaaa gcgtgaaaga cgctattgtg
      541 ctggctcgcg tgacttcatt cgccggtaat ggtaggtagg cttgccgtct ctcccttctc
      601 cttgcgccaa gatcgcagcg atttcttgaa ccttgctatg gtttggcgaa agatggcaca
      661 cgggatcggc ctcccttgca tcgccggtca gggcaaacgc ctgacagcgg cagccgccat
      721 agtcgatgtg tttgcgcggg catgagcggc aaggctcgcg catccattcg accccccgga
      781 agacgttgaa ggctggcgcg ttgagccaag tgtcttcaag cgaatgatct cgcacattcc
      841 agaaaatgag atccggaatg gtttcggcgg catggcaggg aagcgctttg ccagagggcg
      901 tgacgttcaa aatgcgtttg ccccacccgc ccatgcaggc cttgggggtt cgcgcatgat
      961 aatcggggat cacatgatcg atgacgatcg ttccgcaaaa ggacttctta agcgcctcga
     1021 cctcggcgat taccgcttct gcctcagcca agggcggcat cagcgcggcg cgattaagca
     1081 tggcccagcc gtaatattgc acatgtgcga tttcgatccg gcgggcgcct aagcggactg
     1141 cgagctcaac catttttctg gcgcgcgaaa cattagcgcg gtgaatgacg gcatttaccg
     1201 taagcggcag gccctctctc gtgacccatg cggcgaccat ctctttacgc gcgaaggcgc
     1261 cattgtagcc ggcgatacgg tcggccgatt gggcatccga atcctgaatg gaaagctgca
     1321 catgatcgag cccgcaattc gcgagttcgg cgatacgctc ttccgtcacg ccgatgccgg
     1381 aggtgatgag attggtataa aggccttctt tggcgcaatg ggcaacgatc gcgacgagat
     1441 cacgccgtgc cgccggttcg cccccggaaa gatggacatg cagaacgcct agcgccgcgg
     1501 cctcagaaaa aacgcgtttc caaaccccgg tgtcgagttc gttctcgcgc gtatcaagct
     1561 ccagtggatt ggagcaatag gcgcagccca gcgggcagcg atgcgttaac tcagcgagca
     1621 gtccggcggg cgggtcaatt tttgccggcg cagcgctcat agatccacca tgcgcttggc
     1681 cgcaagttct gagagcaacg cccgcacatc cgtctccacc ctggcccgat cggccgagaa
     1741 ttgcccggca agatcatcaa cgattgcccc taatgtggcg gtcccatcgc aacgcttcaa
     1801 tatttctacg gcgattttat cggctttgat gacccgctca ggcgcaagca gaagccattc
     1861 ggcgcgcgta ttgtcatatt tcaaacggac gccgcgcggc agacgcggta tgctatcgct
     1921 ttccgccatg ctcgtgcccg ttgtcatgcg gcttgcccat gtgcgttcgt tcccggcaac
     1981 caggccccgg gcgggatggc gcgatcgaca taagcgctcc acagcgcgtc aagctgcgcc
     2041 caaaggatcg agcatttgaa ttcaagtgcg gcgaggacag ccttttgctc agaccgcgtg
     2101 agagcatgcg tcttcacaaa accgagggca aaatcgctgt cgcgcttggc ttggacaggc
     2161 cgctgcgaaa aataggcgag cgtttcttgg ctgacaaaac cataatgccc gagcatgccc
     2221 gcgacgcgct cgctaataat ggtcggcgaa aacagttccg tcagcgatga ggcgaccgcc
     2281 tcaacgaggc tctttgtccg gcaaaaatcg acataggcat caacagcaaa gcgcgtggcc
     2341 ggcaagatgc cttgcgtcga agccacatag tcgcgcgaaa atccgaggct atccgtcaac
     2401 ttgagccaac gctcgatgcc gccatcgcct tcgcgcaaac cgtcgtgatc ttcgatgcgc
     2461 ttgcgccaag cccgccgatc ggcgggatcc ggcatcctcg tcagaatcgt cgcatccttg
     2521 atcgggatcg tcgcctgata ataatagcgg ttgagcgccc aggcctggac ttcttcataa
     2581 gtacattgtc cgctgtgcag acggcgatgg aaccggtgca gattgtggta acgctcggcg
     2641 ccgatctggc gcaaccgggc ctcgagttcc tcgcccggca tcggctggcc ggcctcgatc
     2701 ataagcacac ctccatacca tcataagcga cctcccagcc cgcccggaga accgcctcgc
     2761 gttcggacga atcctcaatc agaaccgagt tcgtattgtt aatatgcaca taaatcttgc
     2821 gttcgatcct gcagggcgca agcagtttca gcgagccgtt ctcaccgctc atagcaacat
     2881 gccccatgcg ccaggcggtc ttgttcgaca gacccaatgc gatcatttca tcatcggtat
     2941 aggtcgttcc gtcaaaaaaa ataagccttg cgcccgcaag ccgcgttttc aaaccttcgt
     3001 tgatcttggc gcagcccggc acataaaaaa agctcgcggc cccgttgctg atctcaaccc
     3061 cggcggtggc ttcattttcg ccgccgatct caacgtcctc gccttcgaga taaagaggga
     3121 cctttccagg aaccgcgaaa ggcgtgaccg taattccaag gccggtatca accttgcggt
     3181 cgcatgcgac cggcctcgtt tgaaccagat ccttgttcaa tacgccaaaa acccctgcct
     3241 ttgcctgctc cagggtcgct tcgctcccaa agagcataaa actctgctgc tcgcgaagag
     3301 tcaaaagacc ggcgagatga tcgacatcgg cattggtgac aaaaaccgcg caaatgggcg
     3361 agtgcctcgc cccgccccgc ggatgaaggc ttggggaggc tatgatttgc tgacgcagat
     3421 cgggcgacgc attaagaagg agccagcgct ccccatcggc gctaacggcc agactggatt
     3481 ggctgcgcgg ctttacacgc ttatcgccat tccaggcaag ccgacacccc ggacagaggc
     3541 aattccactg gggaaagccg ccgccggccg cggaacctaa gacgataatg cgcaaagcgc
     3601 gcgccaatca caagtttttg gcggaagttc aagcgctcag gtcagatttc agctgacaaa
     3661 tagcttgtta cttccatgcc gatgcagact tcggtgataa cgggagtggt ccaagtcatg
     3721 tcagcccttg cctccatttt taactgttgc gacttttttc agacgcaaac gtttccttgc
     3781 cctgaccagt ttcaactggc ctgcccctat ttagcatcgg ccggagcctg gcgccatgcc
     3841 tttttcaggc aaataaaggc ggccataatg tcatagccac acacgttccg ccgcgacctt
     3901 ggcctcaaag gcttcgatgc tggaagcctt ttgcaaggtc aagccgatat cgtcaagccc
     3961 gtgcaggagg cagtgtttgc gaaacgggtc gatgtcgaag gaaatcaccc cgccgacggg
     4021 tccgcgaatt tcctgggact ccaaatgaac cgagagcgtg gcattagagc cgcgcctgga
     4081 atcgtccata agcttggcga ggtcttccgc cggcactttt atgggcaaaa tcccattc
//
LOCUS       DATMPU010000126         2911 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_98681, whole
            genome shotgun sequence.
ACCESSION   DATMPU010000126 DATMPU010000000
VERSION     DATMPU010000126.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 2911)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2911)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2911
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_98681"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1476
                     /locus_tag="VNY06_02920"
     CDS             <1..1476
                     /locus_tag="VNY06_02920"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HWX13814.1"
                     /translation="MARGESPKTFMDVLTKLFEERFRSPADHVQVLQGHLSGSGRKLV
                     RLTNGKVSAIGVLYNIREENVAFLEFSRYFRRHGLPVPEIYAEDLSQGAYLEEDLGDT
                     TLYEFLSANRAGQNIAPPVIEAYRKVVAVLPRFQVEVGRGVNYKASYPRASFDRQSIT
                     WDLNYFKYYFLRLAGVPFNEQALEDDFGGLTEFLLKADRDYFLYRDFQSRNVMWRDAS
                     PWFIDYQGGRKGALQYDIASLLFDAKADLPPHLRQRLLDEYLARLSDFIPLERDAFMR
                     HYYAYVYVRLMQALGAYGFRGFYEQKTHFLQSVPYALKTLHWLLEHVELPIALPTLMD
                     AFRGMLSSEKLQALPSDAESVSETKHLTVRIFSFSFHRGLPKDETGHGGGFVFDARSL
                     PNPGREERYKPFTGKDAPVIDYLSRQQSVHQFLAGVTSLVDSSLSNYQSRGFKNLMVS
                     FGCTGGQHRSVYLAEELAKHLRARNGLNVVVRHLELEKQGK"
     gene            1473..2228
                     /locus_tag="VNY06_02925"
     CDS             1473..2228
                     /locus_tag="VNY06_02925"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HWX13815.1"
                     /translation="MKAMILAAGLGTRLRPLTDDRPKALVEVGGRTLLEITLARLTSF
                     GIREVIVNVHHFADKIVDYLQANRNFGIDIAISREDVLLDTGGGLKKATYFFLRNSAS
                     AEDPFVLHNVDVISTIDLRRMTQFHLENRALATLAVQDRQTSRYLLFDDRLELCGRKS
                     GVDGTPELVRQHSPQPRPLAFSGIHVISPRFFSLMTEEGVFSIIAPYLRLAAREEKIL
                     GFRSDEYQWRDVGRLEDVTQADRDARSLSAKTR"
     gene            complement(2253..2867)
                     /locus_tag="VNY06_02930"
     CDS             complement(2253..2867)
                     /locus_tag="VNY06_02930"
                     /inference="COORDINATES: protein motif:HMM:NF019846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HWX13816.1"
                     /translation="MNPAAFVAEALADFATVGAIAPSSRYLTQAMLRPLPLENARVVV
                     ELGPGTGVMTRGLLNLLPFDAVLLAFEINPRFARHLKSTITDPRLVVIHARAEALLEE
                     LSRRGYRQVDAILSSLAMGFMSDRQRHALLSEISISLKKKGTFTQYQYLHSMQLRDRQ
                     LTKFDLSGLLEEYFRSVQRKIIWRNLPPAFVFTCRGARVGASAI"
BASE COUNT          590 a          885 c          779 g          657 t
ORIGIN      
        1 atggcaaggg gtgaatcgcc caagactttt atggacgtac tgacgaagct ctttgaagag
       61 cgtttccgct ccccggccga tcatgtgcag gttctccagg gccacctgag tggatcgggg
      121 cgcaagctcg tgcgcctcac aaacggcaaa gtcagcgcga tcggagttct ttacaacata
      181 cgcgaagaga acgtcgcctt ccttgaattc tccagatatt ttcgccgtca tgggctgccc
      241 gttcccgaaa tttatgccga agacttgagc cagggcgctt atttggaaga agacctcggc
      301 gacaccacgc tttatgagtt tctatccgca aaccgtgccg gacagaatat cgcgcccccg
      361 gtgatcgaag cctatcgcaa agtcgtcgcg gtattgcctc gcttccaggt cgaagttggc
      421 cgcggcgtga attacaaggc cagttatccc cgggcaagct ttgaccgcca gtccattacc
      481 tgggatttga attacttcaa atactatttt ctgaggcttg cgggcgtccc cttcaacgaa
      541 caggccctgg aagacgattt cggcggtttg accgaattcc tgttgaaagc cgaccgcgat
      601 tactttcttt atcgggactt ccaatcccgc aacgtcatgt ggcgcgacgc ctctccctgg
      661 tttatcgatt atcaaggcgg acgcaagggc gcgttgcaat acgatatcgc ttccctcttg
      721 ttcgatgcca aggccgatct gccgcctcac ttgcgtcagc ggttgctcga cgaatatctc
      781 gcccggcttt ccgatttcat cccgctcgaa cgcgacgcgt ttatgcgcca ctactacgcc
      841 tacgtttacg tccgcctcat gcaagccttg ggtgcctatg gctttcgtgg cttttatgag
      901 cagaaaactc attttctgca aagcgtgccc tatgcgctga aaaccttgca ttggctgctg
      961 gagcatgtgg aattgcccat cgcgctgcct accctgatgg atgccttcag ggggatgcta
     1021 agttcagaga aactgcaggc tttgccctcc gacgcggaat cggtgtccga aacaaaacac
     1081 cttaccgtgc ggattttcag cttctcattt caccggggat tgccaaaaga cgagaccggc
     1141 catggcggcg gattcgtctt cgacgcgcga agtctgccca atcccgggcg ggaagagcgc
     1201 tacaagcctt tcaccggcaa agacgcgccc gtcatcgact atctcagccg tcagcagagc
     1261 gttcatcagt tcttggctgg cgtcacgtcg cttgtggact cgagcttgag caactaccag
     1321 agccgcggct tcaaaaacct gatggtctcg ttcggctgca ctggaggcca gcaccgttcg
     1381 gtctatctcg ccgaggaact ggccaaacac ctgcgcgccc gcaacgggct caacgtcgtg
     1441 gttcgccatc tcgaactcga gaagcagggc aaatgaaggc catgattttg gccgccggcc
     1501 tgggtacgcg gttgcgcccg ctcactgacg atcggcccaa ggcgctggtg gaggtcggtg
     1561 gccgcacgct gctcgaaatc actctcgcgc gcttgacctc ctttggaatt cgcgaagtga
     1621 tcgtcaacgt gcatcatttc gccgacaaaa tcgtcgatta tctgcaggcg aaccgtaact
     1681 tcggcatcga cattgccatc tctcgcgaag acgttttgct cgataccggc ggcggcttga
     1741 agaaggccac ctattttttc ctccggaatt ccgcctctgc cgaagacccg ttcgttctgc
     1801 acaacgtcga cgtcatcagc accatcgatc tccggcgtat gacccaattt cacctcgaaa
     1861 atcgcgctct ggcgacgctc gcggtgcagg accggcagac cagtcgctac ttgctgttcg
     1921 acgatcggct cgagctttgc ggtcgcaaat ccggtgtgga tggcacgccc gagttggttc
     1981 gccaacattc accccagccg cggccgctgg ctttctccgg catccatgtt atttctcctc
     2041 gcttcttttc gctgatgacg gaagaaggcg tgttctccat catcgctccc tatctgcgcc
     2101 tcgccgcccg ggaagagaaa atcctcggct ttcgttccga tgaatatcaa tggcgggatg
     2161 tcggcaggtt ggaagatgtc acgcaggccg atcgggacgc gagatcgctt tctgccaaaa
     2221 ccaggtaggt tatccgattc gccaaatctt gttcagatag ccgaggcccc gactcttgct
     2281 cctcggcagg tgaatacgaa ggcaggcggc aggttgcgcc agataatctt ccgctgcacg
     2341 gaacggaaat actcctcgag cagtcccgaa agatcgaatt tagtcagttg ccggtcccgc
     2401 aattgcatgc tatgcagata ctgatactgc gtgaaagtcc ccttcttctt gaggctaatg
     2461 ctgatttcgc tcaataacgc atgcctctgc cggtccgaca tgaatcccat cgccagcgac
     2521 gaaagtatcg catcgacctg ccggtacccg cgccggctca gttcctccag cagcgcctcc
     2581 gcgcgcgcat ggatgacaac caggcgcggg tcggtgatcg tcgatttcaa gtgccgcgcg
     2641 aaacggggat tgatctcgaa ggccagcaac acggcgtcaa aaggcagcag atttagcagg
     2701 cctcgcgtca tcactcccgt gcccggcccc agctcaacaa ctacccgcgc gttttccaag
     2761 ggcagcggtc gcagcatcgc ttgtgttaga taacgcgaac tcggcgcgat cgccccgacg
     2821 gtggcgaagt cggccaaggc ttcagcgaca aatgcggccg gattcatttc gttgtctctt
     2881 tcatgcgccc agcttacctc agcattgccc a
//
LOCUS       DATMPU010000127         2513 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_1038982,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000127 DATMPU010000000
VERSION     DATMPU010000127.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 2513)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2513)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2513
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1038982"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            16..1017
                     /locus_tag="VNY06_02935"
     CDS             16..1017
                     /locus_tag="VNY06_02935"
                     /inference="COORDINATES: protein motif:HMM:NF013648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWX13817.1"
                     /translation="MLSRRSLLLFPLALEAAFMESAWASDFIDSSGRTVRLPDSIKRI
                     IPAGPPAEALLYSLAPETLVGLIEFWTDLQRQAGIEKVRDLPTIPRITRKEGTPEAEA
                     IQDLHADIIIDYGSIDARYVTLADKIQSATGIPYLVLDGRLTKVPDIVGRLGSILHRE
                     ERAREIAKTAEFALQKLAPITSKTVGERVSVYYARGSDGLRAIHAGSSLNEGIVLAGG
                     RNVVSPGEGTFSVMSVDEVAALKPSVVILADPKAAERGAPLRKALPTETRFLVDRGLP
                     YGWIERPPSLNRLIGALWLASRLYPDEISFPVDDARRMSVALFHQVPTDATINEMFR"
     gene            1143..1934
                     /gene="modA"
                     /locus_tag="VNY06_02940"
     CDS             1143..1934
                     /gene="modA"
                     /locus_tag="VNY06_02940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018183676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0030973 - molybdate ion binding [Evidence
                     IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HWX13818.1"
                     /translation="MFSLGIAAVALPRPGFAGDAAAPPAVPAGADTGPVVFAAASMKT
                     ALDAVAAAWKANGGKTVSIVYASSAVLAKQIEQGAPADIFISADLQWMDYLDKAKLIR
                     AGTRRNLLGNQLVLIEPSDADSKVKIAQGFDLAGAAGDGKIAVCAIDSCPGGIYAKEA
                     LEKLGVFETVEPKLAQADNIRNALAFVARAEARFGIVYKTDANAEPKVKLVGTFPASS
                     HSPIVYPVALVAASKNPDAASFLAYLSSQAATKIFTGQGFEILSN"
     gene            complement(2231..>2513)
                     /locus_tag="VNY06_02945"
     CDS             complement(2231..>2513)
                     /locus_tag="VNY06_02945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012113084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HWX13819.1"
                     /translation="VVRSGPFVFPGQRHGNLLSPRALEMVLHRMKIEGVTVPGEATSF
                     PREIAEAALAHVAGDATERAYRRGDALEKRRGLMEAWAGFCEPSRMCSR"
BASE COUNT          523 a          757 c          689 g          544 t
ORIGIN      
        1 cctggagtcc ctcaaatgct atcacgccgt tccttgctgc tgttcccgct cgctctcgaa
       61 gccgccttca tggagtcggc ctgggctagc gatttcatcg attcctccgg gcggacggtt
      121 cgtctccccg attccataaa acgcatcatt ccggcgggac cgcccgccga ggcgctactc
      181 tatagtcttg cacctgaaac attggtcgga ttgatcgagt tttggaccga tctccaaagg
      241 caagcgggta tcgagaaggt tcgcgattta ccaactatcc cgcgcatcac gcgtaaagag
      301 gggactcccg aagcggaggc gatccaagat cttcacgctg acatcattat cgattacggc
      361 tccatcgacg cgcgttacgt cacgcttgcg gacaagattc aatccgcaac aggcatcccc
      421 tatctcgtcc tcgacgggag gctcaccaaa gtccccgaca tcgtagggcg cttaggctcg
      481 atcttgcacc gggaagagcg cgccagggaa attgcgaaga ctgcggagtt tgctcttcaa
      541 aaattggcac cgatcacgtc aaagactgtt ggtgagcggg tttccgtcta ttacgcgcgc
      601 ggaagcgacg gcttgcgagc aattcacgcg ggatcgagtc tcaatgaggg aatagtgctt
      661 gccggcggac gcaatgtcgt ttcgccaggc gaaggcacat tttccgtgat gagcgtcgac
      721 gaagtggccg ctctgaaacc ttcggtcgtt attctcgccg atccgaaggc ggcggaacgg
      781 ggcgcgccgt tgcgtaaagc tctaccgacg gaaacgcgct ttctcgtcga tcgcggcttg
      841 ccgtatggct ggatcgaacg gcccccctct ctcaatcgtc ttatcggcgc cctatggctc
      901 gcgtcacgcc tttatccaga cgaaatttcc ttcccggtcg atgatgcccg caggatgagc
      961 gtcgcgctgt ttcatcaggt cccaacagac gcaacgatca acgaaatgtt tcgttgacgc
     1021 caaccatcct acattcgcat agactttctg ttcccagtaa acacgacaga tatggatttt
     1081 ctgaaatgtg tcaaaaagcg agtttgatga ttacggccac acgacgcgga ttggccaccc
     1141 ttatgttcag tttggggatt gcggccgtcg cgcttccgcg ccccggattt gccggtgacg
     1201 ccgctgcgcc gcctgccgtg cctgcgggtg cggacacggg gccagtcgtt tttgcggctg
     1261 ccagcatgaa gacggcgcta gacgcggtcg cggccgcctg gaaggccaat ggcggcaaga
     1321 cggtgtcgat cgtttacgcg tcctccgccg tgcttgcaaa gcaaatcgag caaggggcgc
     1381 cggccgatat attcatttcc gctgacctcc aatggatgga ttatctcgat aaagccaagc
     1441 tcatccgtgc aggaacgcgg cgcaacctct taggcaacca attggttctg atcgagccca
     1501 gcgatgccga cagcaaagtc aagatcgcgc aaggctttga tctcgcgggg gccgcaggcg
     1561 atggtaaaat cgcagtttgc gccatagatt cgtgtccggg cggaatttac gccaaggaag
     1621 ctctcgaaaa attgggggtc tttgagactg tcgaaccaaa gctcgctcag gcggataata
     1681 tccgcaatgc tctagccttc gtcgcgcgtg ccgaagcgag atttgggatt gtctataaaa
     1741 ctgatgccaa tgccgaaccg aaggtgaaac tggtcggcac atttccagca tcgagccata
     1801 gcccgattgt ctatcccgtc gctcttgtcg cggcctcgaa aaatccggac gcggcatctt
     1861 tccttgctta cttatcctcg caggcagcaa ccaagatttt taccgggcag ggcttcgaaa
     1921 ttctgtcaaa ctgacgttcc ctaagctttg caaggaccat cgaaggaccc ctcgaaaccg
     1981 cggggtatcc gcaagagttc cgtatccaac actaacacct tgatttcgtt ctggaacttc
     2041 acgctccgcc aggcctgatt ttctgagacc gacgcctgca cgagctgaca cataatgccg
     2101 gtgtcatttt ccgtggtctt cgctcgcgga gaggcgccgt cattcggcat accatcgcgg
     2161 acaaggttga ccccaagcca agtcacgtct acctgcgatg cggactgtca ctggctcgcc
     2221 tgtggccgtg tcatcgagag cacattctac tcggctcgca aaatcccgcc catgcctcca
     2281 tgagcccgcg acgcttctcc aaggcgtcgc cacgcctgta tgctcgctcg gtggcatcgc
     2341 ccgccacatg ggcgagcgct gcttccgcaa tctcgcgcgg gaaagaggtt gcctcgccag
     2401 gaaccgtcac gccttcgatc ttcatccggt gaagcaccat ttccaacgcc cttggcgaaa
     2461 gcaggttgcc atgtcgctgg ccgggaaaga caaacggtcc ggaccggacc aca
//
LOCUS       DATMPU010000128         5079 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_1346646,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000128 DATMPU010000000
VERSION     DATMPU010000128.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 5079)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5079)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5079
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1346646"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(708..2687)
                     /gene="thrS"
                     /locus_tag="VNY06_02950"
     CDS             complement(708..2687)
                     /gene="thrS"
                     /locus_tag="VNY06_02950"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020175665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HWX13820.1"
                     /translation="MIFVTFPDGSSRQFKSGIRGDEIARSISPSLAKRTVAMVLDGGL
                     RDLADRLTNDARIEFLSRDDPRALELIRHDAAHVLAEAVQTLFPGTQVTIGPVIENGF
                     YYDFYRLEPFTPEDFPAIEEKMTEIIARDKPFAKEVWTREEAKAWFADHGEAFKVELI
                     DTIPANEEVKIYKQGEWLDLCRGPHMTSTGKIGHAFKLMKVAGAYWRGDSTKPMLQRI
                     YATAFANPGELAAYLTQLEEAERRDHRRLGREMDLFHFQEEGPGTVFWHPKGWTLFQT
                     LISYMRRRQSELGYIEVNTPQVLDRALWETSGHWQTFRENMFITQTEDKRVFALKPMN
                     CPGHVQIFKNGLKSYRDLPMKIAEFGIVHRYEPSGAIHGVIRVRAFTQDDAHIFCTED
                     QIMEESFKVNDLILSIYQDFGFKDVVIKLSTRPEKRVGLDDAWDRAEAALSHVLKTLA
                     QQGAKTGVNPGEGAFYGPKLEYTLRDAIGREWQCGTTQIDFNLPGRFGAFYIGPDSEK
                     TTPVMIHRAVFGSLERFAGILIEHYAGHLPLWLSPLQIVVATITQDANDYAYEIMAAA
                     RKRGLLVEGDLRNEKITYKVREHSLAKVPVLFVLGKREAAERSVSIRRLGSQEQTYMK
                     LDEALAMIEAEAAPPDLLRAGQNKIERQMQSVNGG"
     gene            3395..4087
                     /locus_tag="VNY06_02955"
     CDS             3395..4087
                     /locus_tag="VNY06_02955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX13821.1"
                     /translation="MNFLETTSEVCSLRAEEERILASFTRRLIEISAAASGRASGTPP
                     TAALNMMRVPAVMLDEDGFVTDANAAAQAIFDDDVRVKDRRVFLRDCESRAQLKEAIE
                     NLRTTPRLNPLITDPIIVHRQDKLPVILRIWPCYGGANEDAEGMRVVVTLNALGPRPG
                     PPAPILAKTFGLTPSEAKLACIIARGAPPNIAAQELKISRETARNQLKSVFAKTDTHR
                     QSELVALLLQVE"
BASE COUNT         1165 a         1339 c         1332 g         1243 t
ORIGIN      
        1 attgcgtcta catacacata agacacttct ctcaggcaca tacatgtgcc gctctatgta
       61 atgataccgg tgaaggactc ttataagtct tttaacgagc tatcatgcaa cactccattc
      121 acgcagtttt tttgttcgtg accatgtggg cttggtgttt gccctcaacg gggatcgctc
      181 atgcacaaga tgttccggcc atctgtgcga aagccggaaa tgatgaccgg ctgagagtca
      241 taccggccat gctcatccca cgagtgcgct accttttcgg cttctctgca gatgcgcccg
      301 ccgcattggt tcaaaaaagt acatcctatc gatgcatgtc aggagaggtt tggctatgca
      361 attacggcgc taacctcgtt tgtggaaaag ccaatgctac ccgccggtca ccgggtgcca
      421 gtgaattttg caaggcgaac cccggctcgg atgtcgtgcc catggccgcc acgggtcacg
      481 atacgatttt tgagtggaaa tgcgctggaa aagtagccaa aatctccaag caaatcgaaa
      541 ccgtcgatcc tcgcggcttt ctcaccagga attggaagca gttggagcac taaggggcgg
      601 caccggatga tgcggccggc cctatcggac ttcttcgtcg tccatgcggg ggcttagcgg
      661 tcagagaatt cgcccgtttg tagtccatct tcccaaaagt tttcacatta gccgccgttt
      721 acgctttgca tttggcgctc aatcttattc tgcccggcac gcaataaatc gggcggggcc
      781 gcctctgcct cgatcattgc taaggcctcg tcgagcttca tgtaagtctg ctcctgcgaa
      841 ccgagccttc ggattgacac gctgcgttcg gccgcctccc tctttcctaa gacgaaaagg
      901 accggcacct ttgccaacga atgttcgcgc accttatagg taattttctc gttgcgaaga
      961 tcaccctcga caagaagacc tcgtttgcgc gccgctgcca ttatttcata agcgtaatca
     1021 ttggcgtctt gggttatggt cgcgacgacg atttgcagcg gcgagagcca gagggggagg
     1081 tggcctgcat aatgctcgat gagaatgccg gcaaagcgct cgagtgagcc aaaaaccgca
     1141 cgatgtatca ttactggggt cgttttctcg ctatctggcc caatgtagaa agcaccgaag
     1201 cggccgggca ggttgaaatc aatctgcgtc gtgccgcact gccattctcg cccaatggcg
     1261 tctcgcaagg tatattccag cttcggccca tagaaggcgc cttcgccagg attgaccccg
     1321 gttttggcgc cttgttgcgc aagcgttttc aaaacatgtg acagagctgc ctcggcccga
     1381 tcccaagcat cgtccaagcc gacacgcttt tccggtcgcg tcgacagttt gatgacaacg
     1441 tccttgaatc caaaatcctg gtagatcgag agaatgaggt cgttgacctt aaagctctcc
     1501 tccatgattt gatcctcggt gcagaaaata tgcgcgtcgt cctgggtgaa tgcccttacc
     1561 cgtatgacgc catggatcgc accagaaggt tcatagcgat gcacaatgcc aaattcggcg
     1621 atcttcatcg gcagatcgcg ataggacttc agtccgttct tgaagatttg cacatgaccg
     1681 ggacaattca tcggcttgag tgcaaagact cgcttgtcct ccgtctgcgt aatgaacatg
     1741 ttttcgcgaa acgtctgcca atggccagat gtctcccaga gcgcgcggtc aagcacctgt
     1801 ggggtgttga cctcaatgta gccgagctcg ctttgtcgcc gccgcatgta ggaaatgagc
     1861 gtctggaata aggtccatcc ctttgggtgc caaaagaccg tccctggtcc ctcctcttga
     1921 aaatggaaca aatccatttc ccgccctagt cgccgatggt cgcggcgttc tgcctcctcg
     1981 agctgagtta gataggccgc aagttcccca ggatttgcga aagcggtcgc atagatccgc
     2041 tgaagcatgg gtttggtcga atcaccccgc caataggcgc ctgcgacctt catcaacttg
     2101 aaggcatggc caatttttcc ggtcgaggtc atgtggggac cgcggcagag atcgagccac
     2161 tcgccctgct tgtaaatttt cacttcttca ttggccggga tcgtgtcgat gagttcaact
     2221 ttaaatgctt cgccatggtc ggcgaaccag gccttggcct cctcgcgggt ccagacttcc
     2281 tttgcaaaag gtttgtcgcg cgcgatgatc tcggtcatct tttcttcaat tgcgggaaaa
     2341 tcctccggcg tgaaaggttc aaggcgataa aaatcatagt agaacccatt ttcaatcacc
     2401 ggcccgatcg tgacttgcgt gccggggaaa agcgtctgca ccgcttcggc gaggacatgg
     2461 gcggcatcgt ggcggataag ttccagtgct ctcgggtcat cgcggctcaa gaattcgatt
     2521 cgagcgtcat ttgtcaggcg atcggcaaga tcacgcaatc cgccatcaag gaccatggcg
     2581 accgtgcgct tggccagcga aggcgagatg ctccttgcaa tttcgtcgcc cctgatgccg
     2641 gatttaaatt ggcgtgatga gccgtctgga aaagtaacga aaatcatgaa cagtctccca
     2701 gccgctactc gctgcagcca aacagcgtaa gcgcaatccg ttttgagtcg gtagcgtctt
     2761 ccacgttccg cgaattctgt tgttgatgga gggttttaac acaagcgccg cggaaaaagc
     2821 gtcacgcgta agttaccgca agctctagaa tcgtcggaag gatgaagcca tggtgccaaa
     2881 aggaaacggc atttttacgt gtaaaattag ctgcaattat tacgtgttgc aatttcggta
     2941 ccgatatggt tatgatcaat cttatacata gcatcaccta tacaattgtt aatggataaa
     3001 ttacgaattt tgaaactcat aacttgctat agttgaattt ggaggttgtt atgcaacgga
     3061 gcatttgcga atcccacttc tttccttcta aattatttcg caaatgccct gagcgtcacc
     3121 aaatcttctg ttttccattg ggtcatgggc atggctttgc cctcgccgag tcggcttttt
     3181 tgttattttt gaagtcctca cgaaagacaa tactctttac agaactgtca agccttgtta
     3241 ttcatcaaag gcttatgcga taggagttgc tcccgtaact tttaccctaa aagcaagaac
     3301 ggctcagttt ttgaaaccat gggagttaag tcgcagcatt aaatcggctg tttcttgtca
     3361 agatttacgg ggtaattaat tagggaaatt atgaatgaac ttccttgaga caacctcaga
     3421 agtctgcagt ctccgtgcag aagaggaacg aatccttgct tcgttcacgc ggcgcttaat
     3481 cgaaatttcg gcggccgcga gcgggcgtgc ctccggaact cctccaacgg ctgcgctgaa
     3541 tatgatgcgc gtgccggcag tcatgctcga cgaagacggg tttgtcactg atgcgaacgc
     3601 tgccgcccaa gccatattcg acgatgatgt cagggtcaaa gatcgacgcg tctttttgcg
     3661 tgactgcgag tcccgggcgc agctcaagga agctatcgag aatttgcgaa cgacgcctcg
     3721 tcttaatcct ctgatcacgg acccaatcat tgttcaccgc caggataaat taccggtcat
     3781 tttgcgcatt tggccttgct acggcggagc gaacgaagat gccgagggta tgcgcgttgt
     3841 tgtgacgttg aatgccctgg gccctaggcc ggggccgccc gcccccatcc tcgccaagac
     3901 ttttggtctc acgccttcgg aggcgaagct cgcctgcatt atcgctcgtg gcgccccacc
     3961 caacatcgcc gctcaggagc tgaaaatatc ccgggagacg gctcgtaatc agttgaaatc
     4021 cgtcttcgcc aagacagaca cgcatcggca gagcgaactc gtcgctctgc ttcttcaagt
     4081 cgagtgacct agccgactcc aatttgctct cggcgtgacc gtgcgtttcg gcggatcgcg
     4141 cgaaccggca gtcctgagcg cgatcttctg cccccatgca gcaagcgaca tacagttttc
     4201 attcgcggtc ccgatgcctt caaacggggc tttgagaggt tgactaaaaa atcgcctagc
     4261 gcgcgagtcg tatgactgcg atctggatca cggcgagagt gatgaaaatt tgaaataacg
     4321 cggcgggatt ttcccactcc ttggcgaggc gtcgattacg accaaaccat gagaaaattc
     4381 tgcgatccaa gagtgcggca acataatgaa gcgttttgtc tcatcgatgg ggctaatgat
     4441 ttcgatgcgc agcgacgtcg ggggaggcga gcacggacca aatcataacc aaaattggga
     4501 atggagttga cgcaggaatt tagccaaggc aggcgtgctc cggcgcataa agacttggct
     4561 tgcaattttt ttcgcttgtg gtgccagggc acgcctgtac gccaaaccca aagaatgtaa
     4621 ccgcctgtta cttgttctcc atttcccgcc gcgcgcccgc cctttcggca agcggaacga
     4681 agacgcgccc ttttcagggt cttttaagat cactctgatc caatgctgct ttctgcggtg
     4741 acgcgagatt ttcctgcaca cccatagtcg gagttgtgtt cggcttgatc gcacccgagc
     4801 ggattagggt catttcgaat tttaggggct gaaggcattc gaacggccaa gagccacagc
     4861 tcggagtttg aagcggagcg caatgcgttt atcgccaaag cgcaggagct gatgcgcgag
     4921 caccagcttg ggataggaga ggtcgaggct gcaaatcctg ataatcccct tggctcagat
     4981 attaatcaag ggctcatagg tgcactggaa acagataaaa tcaaggggtt agctgtaaaa
     5041 attgaatggc tgataagtta tataaattgc ataatgagt
//
LOCUS       DATMPU010000129         4755 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_2271831,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000129 DATMPU010000000
VERSION     DATMPU010000129.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 4755)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4755)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4755
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2271831"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            153..2150
                     /gene="tkt"
                     /locus_tag="VNY06_02960"
     CDS             153..2150
                     /gene="tkt"
                     /locus_tag="VNY06_02960"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009763426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HWX13822.1"
                     /translation="MLAQDKKLNHDDMANAIRALAMDAVEKAKSGHPGLPMGTADIAT
                     VLFTKVLKYDAAAPDWPDRDRFVLSAGHGSMLLYALLYLTGEPSMTIDELQRFRQLGS
                     LTPGHPEYRHTPGVETTTGPLGQGLGTTVGMALAERMLNAEFGSIVDHRTYVLASDGD
                     LMEGISQEAIAIAGHLRLNRLIVFHDENHVSIDGPLSLADSVDQVKRFQACNWNAVRI
                     DGHDQAAILRAIRKAQKSEKPSLIACKTIIGFGMPTRAGTSKAHSDAPGPEEIAGARR
                     NLNWPYEPFFVPQPILAAWRKAGKRGRPRRKAWNAALQLLPTAAREEFERRLRGDLPA
                     SFGEAILGYKRKLAEERAPVATRKASQAALEVLAKAVPELITGSADLTPSNNTKVSAT
                     PEIGPASYGGRYLHWGVREHGMAAAVNGIAVHGGFIPSGATFLIFSDYCRPALRLAAL
                     MGIHVIQVFTHDSIGLGEDGPTHQPVEHLAALRAIPNMLVFRPADQQETLECWQLALE
                     AKTSPSILALTRQNLPPVRNVFTPENLSAAGAYEVAPSEGKPAKVSIFASGSEVSIAL
                     GARSILAESGIAARVVSVPCMDLFLKQDQTKRREIIGDAPIRVAVEAAVRQGWDEIIG
                     EDGIFVGMTGFGASAPYRDLYNRFKITPEAVAEAARLRCNN"
     gene            2230..3237
                     /gene="gap"
                     /locus_tag="VNY06_02965"
     CDS             2230..3237
                     /gene="gap"
                     /locus_tag="VNY06_02965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011924279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HWX13823.1"
                     /translation="MAIRVAINGFGRIGRNILRAIVESKRKDIQIVAINDLGPVETNA
                     HLLRFDSVHGRFPDEIKIGKDSIDCGAGPIKVTAIRDPRELPHRELAVDIALECTGFF
                     TSKDKASAHLKAGAKRVLISAAADHADLTVVFGVNHAKLTKDHLIVSNASCTTNCLAP
                     VAKILNDAIGIETGFMTTIHSYTGDQPVLDTMHKDLYRARAAALSMIPTTTGAARAIG
                     LVLPELNGKLDGAAIRVPTPNVSVVDFKFVAKRKTTAEEIKETVKQASQQQMKGILTF
                     TESPNVSADFNHDPHSSIVHMDQTKVMDGTFVRVMSWYDNEWGFSNRMADVAAAMGKL
                     I"
     gene            3234..4463
                     /locus_tag="VNY06_02970"
     CDS             3234..4463
                     /locus_tag="VNY06_02970"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012386372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HWX13824.1"
                     /translation="MTRPAPFYVFRTLDDIDVAGKRVLVRADLNVPMEDYRITDTTRI
                     ERVLANIREISDRKAKVIILSHLGRPKGGPDAKNSLKPVAVEFERQLGRYIGFASDCA
                     GDVAKSAVQAMKDGDILLLENTRFHDAETKNDPAFVTALAELGDIYVNDAFSTAHRAH
                     ASTEGLAHKLPAYVGRSMQLELEMLKRMLADPKRPLAAIVGGAKVSTKFELLGNLMRQ
                     VDSMFVGGGMANTLLLASGKEVGRSLCEPKLTDAARKILADADSAKCRLVLPVDVIVA
                     TALEPDTRTKTVDVDSVSEDQMILDIGPRTISQAVGILASARTLVWNGPVGAFEVAPF
                     ETGTLTIAKVAAQLTKVGALESIAGGGDTIAALNQAGVAGDFTYVSTAGGAFLEWLEG
                     KKLPGVEALRWASAKSA"
     gene            complement(4516..4671)
                     /locus_tag="VNY06_02975"
     CDS             complement(4516..4671)
                     /locus_tag="VNY06_02975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX13825.1"
                     /translation="MQLLFPIHFTATIIGETDLLLSMTALRAAAGGGHEAIIDKGGPL
                     AKFAAKR"
BASE COUNT         1004 a         1420 c         1365 g          966 t
ORIGIN      
        1 ttaaagagaa ctataaggaa ccacaggcaa agcattgaca caagtgtttt ggaggttacc
       61 cggctagact tgcctagttt tatccaccgc aggctgctca aagggcgcac gacgccaaca
      121 tgctctaaaa atcttcgttg ggtgaacagg tgatgctcgc gcaggacaaa aaactaaacc
      181 atgacgatat ggcgaatgcc attcgcgcgc tcgccatgga tgcagtggaa aaggcgaaat
      241 ccggtcatcc ggggctcccc atgggcaccg ccgatatcgc cacggtgctg ttcaccaagg
      301 tgctcaaata tgacgccgct gccccagatt ggccggatcg cgaccgtttt gtcctgtccg
      361 ccggtcatgg ctcgatgctg ctctatgcgt tgctttatct gaccggcgaa ccgagcatga
      421 ccatcgatga actgcaacgc ttccgccagc tcggatcgct gacgccgggc catcccgaat
      481 accgccatac gccgggggtg gagacgacca cggggccgct cggccagggg ctcggcacga
      541 cagtcggcat ggcgcttgcc gaacgcatgc tgaacgccga attcgggtcg atcgtcgacc
      601 atcgcactta tgtgctggcg tccgacggcg atctcatgga ggggatcagc caggaggcaa
      661 tcgcgatcgc cggtcacctg aggcttaacc ggctcatcgt gtttcatgac gaaaatcacg
      721 tctcgatcga tgggcctctc tcgctagcgg attccgtcga tcaggtgaag cggtttcaag
      781 cgtgcaactg gaacgccgtc cgcatcgatg ggcatgacca ggccgccatc ctgcgggcga
      841 tccgcaaggc gcaaaagtcg gaaaagccga gcctcatcgc ctgcaagacg ataatcggct
      901 ttggcatgcc gacgcgggcc ggcacatcga aggcccatag cgacgccccc ggcccggagg
      961 aaatagccgg cgcgcggcgc aaccttaatt ggccctacga gccctttttt gttccgcagc
     1021 ctattttggc cgcctggcgc aaggccggca aacgcgggcg cccgcgccgc aaggcctgga
     1081 acgcggcgtt gcaacttctg cccacggccg cgcgggaaga gttcgagcgc cgtttgcgcg
     1141 gcgatttgcc cgcctctttc ggcgaggcca ttttaggcta caagcgcaag ctcgccgagg
     1201 aacgtgcgcc tgtcgccacg cgtaaagcct cccaggccgc actcgaagtt ctggcgaagg
     1261 ccgtaccaga gctcattact ggatcggccg atcttacccc ttcgaacaac accaaagtct
     1321 cggcgacgcc agagattgga cctgcatctt acggtggtcg ctatctccat tggggtgtgc
     1381 gcgagcacgg catggccgcg gccgtcaatg gcatcgcggt acatgggggc tttattcctt
     1441 ccggcgcgac atttttgatt ttcagcgatt attgccgccc ggccttacgc cttgccgccc
     1501 tcatgggcat tcatgtgatc caggtcttta cgcatgattc catcggcctt ggcgaggatg
     1561 gcccaaccca tcagccggtt gagcacctcg ccgcgctgcg cgctattccg aacatgcttg
     1621 ttttccggcc ggcagatcaa caggagacgc tggaatgctg gcagcttgcg ctcgaagcca
     1681 aaacctcgcc ttccattctc gcgctgacac ggcaaaatct cccgcccgtt cgcaatgttt
     1741 tcacccccga aaatctatcg gcagccggag cctatgaagt tgctccctcg gagggcaaac
     1801 cggcaaaagt ttcaatcttt gcgtccggtt cggaagtctc gatcgcactt ggcgcgcgaa
     1861 gcattctcgc cgaaagtgga attgctgcgc gagttgtttc ggtgccttgc atggaccttt
     1921 tcttgaagca ggatcaaacc aaacggcgag aaattattgg cgatgcgccc attcgcgtcg
     1981 ctgttgaagc agcggtccgt caaggttggg atgagattat tggcgaagat gggatttttg
     2041 tcggcatgac aggttttgga gccagcgcac cttacagaga cctttacaat cgctttaaaa
     2101 tcacgccaga agccgtcgca gaggcagcgc gtttgcgctg caataattga cccttaatct
     2161 ttattaacgc ataccacggc gctcggggca gcgcaacatt tctcggttca ccgccaggaa
     2221 ggagaagata tggcgatcag agtcgctatc aacggttttg gccgcattgg ccgcaatatt
     2281 ttgcgcgcaa tagtcgagtc gaagcgcaag gacattcaga ttgtcgcgat taacgatctt
     2341 ggccctgtcg agaccaacgc ccatctgctg cgcttcgatt cggtgcacgg ccgttttccg
     2401 gacgagatca agatcggcaa agactcaatt gattgcggcg ccgggccaat caaggtgacc
     2461 gcgatccgcg atccgcgcga attgccgcac agggagcttg ccgtcgacat cgcgctcgaa
     2521 tgcactggct ttttcacctc gaaggacaaa gcctccgcgc atttgaaggc gggcgccaag
     2581 cgcgtcctta tctccgcggc ggccgaccat gccgatctga ccgtggtctt tggggtcaat
     2641 cacgccaagc tgaccaagga tcatctgatt gtctccaacg cgtcctgcac caccaattgc
     2701 ctcgctccgg tcgccaaaat cctaaacgac gcgatcggca tcgaaacggg gttcatgacc
     2761 acgatccact cctacaccgg cgaccagccg gtgctcgaca caatgcataa ggacctttac
     2821 cgggcccgag cggccgcgct ttcgatgatc ccgaccacga ccggggcggc gcgggccatc
     2881 ggcctcgtcc tccccgagct caacggcaag ctcgatggcg ctgcgatccg cgtgcccacc
     2941 ccaaacgtgt ccgtcgtcga tttcaaattc gtcgccaagc gcaagactac ggccgaggaa
     3001 atcaaggaga ccgtcaagca agcttcacag cagcagatga aaggcatcct tacctttacc
     3061 gaaagcccta acgtgtcggc ggatttcaat cacgacccgc attcgtcgat cgtccatatg
     3121 gatcagacaa aggtcatgga cgggacattc gtccgcgtca tgtcttggta cgacaatgaa
     3181 tggggatttt cgaaccgcat ggccgatgtc gctgccgcca tgggaaaact catatgacga
     3241 gacccgcacc tttctatgtt tttcggacgc tcgacgatat cgatgttgcg ggcaagcgcg
     3301 tgctcgtgcg cgccgatctc aatgtcccca tggaggatta caggatcacg gatacgactc
     3361 gcatcgagag ggtgcttgca aatattcgcg agatttcgga ccgaaaagcc aaagtcatca
     3421 ttctctcgca tctcgggcgc ccaaagggcg gccccgacgc gaaaaattcg ctcaaacccg
     3481 tcgcggtgga atttgagcgc cagctcggac gctatatcgg ctttgcctct gactgcgccg
     3541 gagatgtcgc gaagagcgct gtccaagcga tgaaggacgg cgatattctt ttgcttgaaa
     3601 atacaaggtt ccacgacgcc gagacaaaga atgatccggc ttttgtcacg gcgcttgccg
     3661 agctcggcga catttatgtc aatgatgcct tttcgaccgc gcatcgcgcc catgcctcga
     3721 ccgaggggct cgcgcataaa cttccggcct atgtcggccg cagcatgcag cttgagcttg
     3781 aaatgctcaa acgcatgctg gccgacccaa aacgcccgct cgcggcgatt gtcggcggcg
     3841 ccaaggtctc gaccaaattc gagctcttgg gcaatctcat gcgtcaggtc gactctatgt
     3901 ttgtcggtgg tggcatggcc aatacgctgc tgcttgcgag cggcaaggag gtcggccgat
     3961 cgctctgcga gccgaaactc actgacgccg cccgcaaaat tcttgccgat gcagattcgg
     4021 cgaagtgcag actcgtgctt cctgtcgatg tgattgtcgc gacggcgctt gaacccgata
     4081 cgcgcacaaa gacggtggat gtcgattccg ttagcgagga tcagatgatt ctcgacatcg
     4141 gccctcgcac catctcccag gcagtcggga tccttgcctc agctcgcacg cttgtctgga
     4201 acgggcccgt cggggccttt gaggtcgcgc ctttcgagac cggcacgctg acgatcgcga
     4261 aggtcgcggc gcagctcacc aaagtggggg cgctcgaatc catcgccggc ggcggtgaca
     4321 cgatcgcggc gctcaaccag gcgggcgtcg cgggcgattt cacttatgtg tcgacagcgg
     4381 gcggcgcttt tctcgaatgg ctcgaaggca agaaactgcc gggcgtcgaa gcgctgcgct
     4441 gggcgagcgc aaaaagcgcg tgaggtgtgc agcattagaa ttcttaactt gacgtaggtt
     4501 atcagggtcc gccattcatc tcttggcggc gaatttggcg agcggtccac ccttgtcaat
     4561 gatcgcttcg tgaccgcctc cggcggcggc ccgaagagcc gtcattgaca agagcaggtc
     4621 cgtctcgccg ataatggtcg ccgtgaaatg aatgggaaat agcagctgca aacagctgag
     4681 cctgaaatct caaatccaaa gtaaagacag gtgatggccg aaatcgtttt tgtgcatggc
     4741 atagaccagc agcaa
//
LOCUS       DATMPU010000130         5190 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_725940,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000130 DATMPU010000000
VERSION     DATMPU010000130.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 5190)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5190)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5190
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_725940"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..299)
                     /locus_tag="VNY06_02980"
     CDS             complement(<1..299)
                     /locus_tag="VNY06_02980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003609360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HWX13826.1"
                     /translation="MNLPTHPALDRLQFFRVNAAFARLRSLFVLASFGLAVSGCGYNT
                     IPTLEEQAKARWGDVQNQYQRRADLIPNLVATVQGYAKQEKEVLTSVVEARAKATS"
     gene            494..1363
                     /locus_tag="VNY06_02985"
     CDS             494..1363
                     /locus_tag="VNY06_02985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015928997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HWX13827.1"
                     /translation="MNPELEHLTFDLADVSVHAVAAGPQDGPLAILLHGFPEFWYGWR
                     SQIMPLAKAGFRVVVPDQRGYNQSSKPKAWQAYAVPCLVGDVLGLADSLECEHFMLAG
                     HDWGGIIAWACAMEHEDRVGRLAILNAPHPSVFWKYARTHPAQVLRSWYVFYMQLPWL
                     PEFLFEAANFRMMTETLAKTSRPGTFSELDFEAYREAWRQAGAPAGMINWYRALRAST
                     PSGNPTLEIPVRILWGARDHFLAPDLAERSLRYCRRGKLIVFPEATHWLQHEEAATVS
                     NALTDFFSGAGKA"
     gene            1406..2113
                     /locus_tag="VNY06_02990"
     CDS             1406..2113
                     /locus_tag="VNY06_02990"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HWX13828.1"
                     /translation="MPSAAIRFIVGKLRHLTNQRIAAIGRLCSHHDYDQIWRSDGYLE
                     KFHKVKPQTIARNRLNLGEFGTHVECLRLAALLFSAPDVKRVMDVAAGSHWQFPIAYK
                     GAYGLELSGVDIDADAVGANDSLDFRFVDDVCRNSRIGIGNYDLMTSYSGVEHFPDVQ
                     KFLETAFISLKPGLSSDAKGGALIAQFPSPLAPFAILNRLLPQGLKRKIINTLYSDKA
                     DELGYAVSYDKCRYSEF"
     gene            complement(2333..3331)
                     /locus_tag="VNY06_02995"
     CDS             complement(2333..3331)
                     /locus_tag="VNY06_02995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003611585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HWX13829.1"
                     /translation="MELSIIEQNPEPPGGSVLAITTRDNKTLRAAHWSCGENCVGTVA
                     ILPGRTEFIEKYFEVIRELLSRNFDVAILDWRGQGGSARLTKNSRKGHVGDFRAYERD
                     LDAFAEEVLALHCRPPWFALGHSMGAAIALAKARSGRSPFARMVLTSPMIDLCGLRFR
                     RTLQVLARVLVLSGQGRRFVPWGGANRSLQEGFQGNILTSDEKRYARNAAIIEAAPEF
                     TIEDPTVGWINAAFRLMRRFKEEQFPRRLLTPVLILAAGADRLVDTAATEEFAHRLKA
                     GKCIVLKGAEHEILMETDPLRELFWAAFDAFLPGEHAIGDQPGRQHDRDNSGGNRS"
     gene            3596..4066
                     /locus_tag="VNY06_03000"
     CDS             3596..4066
                     /locus_tag="VNY06_03000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020186938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20 family protein"
                     /protein_id="HWX13830.1"
                     /translation="MRQFDLSPLYRSTIGFDRLFNMIDQAAGFEAAPTYPPYDIERTG
                     ENAYRISVAVAGFAANELSIETKENALTIRGSRERETESGTADREILHQGIAARAFER
                     RFQLADHVQVTGASLENGLLHVDLFRELPEAQKPRQIPITTEGAKPTVSADKAA"
     gene            complement(4216..>5190)
                     /locus_tag="VNY06_03005"
     CDS             complement(4216..>5190)
                     /locus_tag="VNY06_03005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020173422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-eliminating lyase-related protein"
                     /protein_id="HWX13831.1"
                     /translation="KDPWSAKAARLVREAFDRDCTCFLVNTGTAANALALAAICPPFG
                     AIFCHTESHITEDECGAPEMFTGGAKLIGISGHSGKIAPAELKAVIAQYPRGVDKQVQ
                     PAALSLSQATESGTIYSCDELALLAALAHDAGLMVHMDGARFANAIVGLKAIPAEMSW
                     KAGIDVLSFGATKNGALGCEAVIFFDPAKGESMPYLRKRSGHTLSKGRFLGAQMTAYL
                     ENEHWLDLAAVANAHARDLAHGLAKIPGVRMPWPCEANEIFVILPRSTDAALRAGGAV
                     YYPWKSRDFGPRCEPPARDEVFVRLVTSFATKPSDIENFLVIAAGAGR"
BASE COUNT         1092 a         1483 c         1481 g         1134 t
ORIGIN      
        1 gaggtcgcct tggcccgtgc ctcgacaact gaggtcagca cctccttctc ctgcttggcg
       61 tagccttgaa cggtcgcaac cagattggga atgagatcgg cgcggcgctg atattggttt
      121 tggacatcgc cccatctggc cttggcctgc tcttcgagcg ttgggatcgt gttgtagccg
      181 cagcccgaaa ctgcgagccc aaaagaggcg agaacgaaga gtgagcgcag gcgcgcaaag
      241 gccgcattca cgcggaagaa ttgtaggcga tcgagcgccg gatgtgtagg caagttcatg
      301 agcgaaacct ccagtactgg tcaaactaac cggttcgggc aatttgggca aaggcgcgag
      361 gaacgccgcg cttgtcagtc ctccggaatg gccgttgtta agttggcggc aaatctcgca
      421 aagttttatg cgccgcgcaa acgcacggcg tgttatagcg ggaccttgta gccacagcgc
      481 gtggaaatgg ttcatgaatc ccgagctgga gcatctgact tttgacttgg ccgacgtgtc
      541 ggtccatgcg gtcgcggcgg gcccgcagga tggccctttg gcaattctcc ttcatggatt
      601 tccggaattc tggtacggct ggcgcagcca aatcatgccg ctcgccaaag caggttttcg
      661 cgtagtcgtc ccagatcagc gcggctataa ccaaagcagc aagccaaagg cttggcaggc
      721 ctacgcggtg ccgtgtcttg ttggcgacgt tcttggcctc gcggacagct tggagtgcga
      781 acattttatg ctcgcgggcc atgattgggg tggaatcatc gcctgggcgt gcgccatgga
      841 gcatgaagac cgagtcggcc gccttgcaat cttgaatgcc ccgcatccaa gcgtgttctg
      901 gaaatatgcg cgtacgcatc cggcgcaagt cttgcgcagc tggtatgtgt tttacatgca
      961 acttccctgg ctgccggaat ttctattcga ggccgcaaat ttccggatga tgacggagac
     1021 actcgccaaa accagccgtc caggaacctt ttccgaactg gattttgagg cctatcgcga
     1081 agcgtggcgg caagctggag cgccagctgg catgatcaac tggtaccgcg ccttgcgcgc
     1141 ctcaacgcca agcggcaatc ccacgcttga aattccggtg cggattcttt ggggcgcgcg
     1201 ggaccacttt ctcgcgcctg acctcgcgga acgaagcctg cgttactgcc ggagaggcaa
     1261 gctgatcgtg tttcctgagg ccacgcactg gttgcagcac gaggaggcgg ccacagtctc
     1321 gaatgcgctc acggattttt tttcgggcgc gggtaaggct tgacacgcgc ccgaggccta
     1381 tcgccggctg taaactgcgg tgcgattgcc gtccgccgcg attagattca tagtcgggaa
     1441 acttcgccat ttaaccaatc aacggatagc agccatcgga cgtctttgtt ctcaccatga
     1501 ttacgatcaa atttggagaa gcgatggata tcttgagaag tttcataaag ttaaacctca
     1561 gactatcgcg cgtaaccgcc ttaacttggg agagtttggg acgcatgtgg aatgcttgcg
     1621 cttggcagca ctcttatttt cagctcccga tgtaaagcga gttatggatg tcgccgccgg
     1681 ttcgcactgg caatttccaa tcgcctataa aggcgcctac gggctcgagc tctcgggcgt
     1741 tgatattgac gcggacgcgg ttggcgccaa tgattcgctt gattttcgat tcgtagatga
     1801 cgtctgccgc aattccagaa tcggcattgg aaactatgat ctcatgacaa gttattcggg
     1861 cgtggagcac tttccagacg ttcaaaagtt tctggagact gcgttcattt ccctaaagcc
     1921 gggcctgagt tctgacgcga agggcggcgc gctgatcgcg caatttccct cgcccctcgc
     1981 tccttttgct atactcaaca gactattgcc gcaaggtctg aaaaggaaaa taataaatac
     2041 cttgtattct gacaaagctg atgaactcgg ttacgccgtt tcatatgata aatgccgtta
     2101 ctcagagttt taaggcagcc gctgaacgct cgggatttca agtggagtat tatctatgct
     2161 cgtatatgtc tagcggctac ttcaactggt ttctgccttt atttttattg tcgcagctac
     2221 ttgatattgt tcggctcagc ttcggaacga aggatatggc gtcctacaat ctttttgtgc
     2281 tgcgccgtcc tggcccgcat tttgagatca aatggtcatg gcacgccagt aactaacttc
     2341 tgttcccccc gctgttatcc cgatcatgtt gtctcccggg ttggtccccg atcgcgtgtt
     2401 cgccggggag aaaagcatcg aaagccgccc aaaagagctc gcgcaaagga tccgtctcca
     2461 ttaggatttc gtgttcggcg ccctttagga caatgcattt tccggccttg aggcggtggg
     2521 cgaattcctc cgtcgcggca gtatcaacta agcggtcggc gccagcggcg aggatcagga
     2581 ctggcgtcag aagccggcgc ggaaactgct cctccttgaa gcgtcgcatg aggcgaaagg
     2641 cagcgttgat ccaaccgact gtaggatctt cgatggtgaa ctcgggcgcc gcttcaatta
     2701 tcgcggcatt ccgggcgtag cgcttttcat cagaggtcag gatattgccc tgaaaacctt
     2761 cctgcaggga gcggtttgcg ccgccccaag gcacgaaacg gcggccttga ccggataaga
     2821 cgagtaccct ggcaaggacc tgcagggtgc ggcggaagcg aagcccgcaa agatcgatca
     2881 tcggggaggt gaggaccatg cgggcaaaag gcgaacggcc agaacgcgct ttcgcaaggg
     2941 cgatcgccgc gcccatcgaa tggccaagcg caaaccacgg aggccgacag tgcagcgcga
     3001 ggacttcttc cgcaaaggca tcgagatcac gttcataggc tcggaaatcg ccgacatggc
     3061 ccttacggct gtttttggtg agacgggccg agccaccctg cccgcgccaa tccaagattg
     3121 cgacatcgaa gttccggctt agcaactcac gaataacctc gaaatatttt tcgatgaatt
     3181 ccgtgcgtcc gggaaggatc gcgaccgttc ccacacaatt ctcgccacag ctccagtgtg
     3241 ccgcccgcaa ggttttatta tcccgcgtcg tgatcgccaa gacagagccg ccgggcggtt
     3301 ctggattttg ttcaattata ctcagttcca tcaataaatt ggaacctctc gggtccaatc
     3361 gcgatccgtc cgagtccccg acgagtgctc gcactgcgcc cccattatcg gttgccttct
     3421 ttcccctctt gcaaagccaa accctatccc taaatcagaa tggcgcgggc cccttacgga
     3481 cgcgcgggcg gaaacaaaac cttggcgcag cgagaacgta gctggctaag cctgttgttc
     3541 tgctcggcaa caatctttcg tgcaacacct cgcttcattc aaggaggatt aggaaatgcg
     3601 tcagtttgat ctttctcccc tgtaccggtc gaccatcggc ttcgaccggc tgttcaatat
     3661 gatcgaccag gcggccggat ttgaagccgc gccgacttac ccgccctacg atatcgagcg
     3721 caccggcgaa aacgcctacc ggatttcggt agcggttgct ggctttgcgg caaatgagct
     3781 ttcgatcgaa accaaggaaa atgctctaac cattcgtggt tcgcgcgaac gtgagaccga
     3841 aagcggcaca gcagatcggg aaatccttca ccaaggcatt gccgcccggg ccttcgaacg
     3901 ccggttccag ctcgccgatc acgtgcaagt gaccggcgca agccttgaaa acggcctgct
     3961 tcacgtcgat ttgtttcgtg aactcccaga ggcgcaaaaa ccgcgccaga taccgatcac
     4021 aaccgagggc gccaagccaa cggtctcagc ggataaggcg gcgtaactgc gctagccggg
     4081 cggctcccct cccgcgcccg gtggttgtca gggcgtctcg caagaggcgc ccttttttgg
     4141 tttttgaacc ctcgaattga accgttacca gaccgaggag gcttgggcga agcgggcgga
     4201 ttgcccgacc ggtgctcaac gaccggcgcc tgccgcaatg accaggaagt tttcgatgtc
     4261 gctaggcttc gttgcaaacg aggtaaccag ccggacgaaa acctcgtccc gggccggcgg
     4321 ttcgcagcga ggaccgaaat cacgagattt ccaagggtag tagaccgcgc cccccgctct
     4381 tagcgcggcg tcggtggagc gcggcaaaat cacgaaaatt tcattcgcct cgcagggcca
     4441 tggcatgcgc acaccgggga tcttcgctag cccatgcgcg agatctcgcg catgggcgtt
     4501 tgccaccgcg gcgagatcga gccagtgctc gttttcaaga taggcggtca tttgcgcgcc
     4561 aagaaagcgt cctttggata gtgtatgccc gctgcgcttg cgcaggtagg gcatgctctc
     4621 tcctttcgcg ggatcaaaga atataacggc ttcgcagcct agagcaccat tcttggtggc
     4681 gccgaaggac aaaacatcga tgcctgcctt ccagctcatt tcggccggaa tggctttcag
     4741 gccaacgatg gcattggcga agcgggctcc gtccatgtga accatgagcc ctgcgtcatg
     4801 cgcaagcgcc gcgagcagag caagctcgtc gcagctataa atcgtccccg attcggttgc
     4861 ctgagaaagg gacagggccg cgggttgaac ttgtttgtct accccgcgcg gatattgcgc
     4921 gatgaccgcc tttagttccg ccggcgcgat cttgcccgag tggcccgaaa ttccgataag
     4981 cttcgccccg cccgtgaaca tttctggtgc gccacactca tcctccgtaa tgtgcgattc
     5041 agtgtggcag aaaatcgctc caaagggcgg acagattgcg gcaagcgcca gagcatttgc
     5101 cgcggttccg gtgttcacca agaaacacgt gcaatcccgg tcaaatgctt cgcgcaccag
     5161 ccgtgcggcc tttgcgctcc aaggatcctt
//
LOCUS       DATMPU010000131         7671 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_3204996,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000131 DATMPU010000000
VERSION     DATMPU010000131.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 7671)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7671)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7671
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3204996"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            388..669
                     /gene="yajC"
                     /locus_tag="VNY06_03010"
     CDS             388..669
                     /gene="yajC"
                     /locus_tag="VNY06_03010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012384000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HWX13832.1"
                     /translation="MIMQLVPFAIIFVIAYVLVIRPQRKRSKQQADLLKNVRRGDNIV
                     MSGGLIGKITRVVDDNEIELEIAPSVRVRAARAMISEVRSKGEPVKDQG"
     gene            753..2348
                     /gene="secD"
                     /locus_tag="VNY06_03015"
     CDS             753..2348
                     /gene="secD"
                     /locus_tag="VNY06_03015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018408105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HWX13833.1"
                     /translation="MLRFATWKIASILAMTLFAFLLIVPSLLGPGRRDALISFMPKWL
                     PVRAIVLGLDLQGGSHVLLEVDKNSVIKTLVDNLRDSVRRVLREEKVPITGGIGALPR
                     GVQVRIPDPSEREKAMPKLRALAASSGGVLSAADRAPTFDVTESDNGLIQFTATEAGI
                     ESKVRRAVEQSIEVLRRRVDALGTTEPNIQRQGDDRVLVEVPGLQETSKLKEILGTTA
                     KLEFRLVAEPGADPAEVELLDEVEEGKKIPIQKRVMVQGEDLTDAQPGFDPRTQEPVV
                     NFRFNIRGGQRFGEVTAENVGKLFAIILDGKVISAPRIISPITGGSGQISGHFTVEQA
                     NNLAILLRAGALPAKLTIVEERTVGPGLGQDSIDAGKRAAYVGAAFVAFYMLITYGIF
                     GVFANVALFVHISFIFAGLVLLGATLTLPGIAGIVFTIGMAVDSNVLIYERIREEAHM
                     GRSILSALDAGFKRAFATIVDSNVTMFVAAAILYFLGSGPVRGFAVSMALGILTSIVT
                     AVTMTRMMIALWYRYQRPTKLPI"
     gene            2482..3423
                     /gene="secF"
                     /locus_tag="VNY06_03020"
     CDS             2482..3423
                     /gene="secF"
                     /locus_tag="VNY06_03020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003611019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HWX13834.1"
                     /translation="MKLLRLAPENTKFPFMRFRRVSYPFSALLSIIAVVLFINLGMNF
                     GIDFSGGTLIELRAKNGAADLAKLRSLAEHMDVGGVEVQGFGGPSDVTLRFGLQAGGD
                     VTQQAAVNRVISAIGSDYEVRRTEVVGPRVSGELVQSGTLGVVIAILAVLCYLWFRFE
                     WQFAVGAIIATMHDLLLTVGFFALTRLEFNTTSIAAILTIVGYSLNETVVVLDRIREM
                     MRKYRKMPTDQLIDVSVNAVLPRTIMTATTVFLALLSLALFGGEVIRSFSLAMIWGIF
                     VATYSSIFICSPMLIYLGLRADTVGPAPAATPTKTPA"
     gene            3473..4342
                     /locus_tag="VNY06_03025"
     CDS             3473..4342
                     /locus_tag="VNY06_03025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015928859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene/squalene synthase family protein"
                     /protein_id="HWX13835.1"
                     /translation="MMAAADSLTKGSDYAYCEDLIRRNDPDRWLASLFVPQDRRRHIH
                     ALYAFSLEIARVREAVSEPLLGEIRFQWWRDAHEGKNAGDARANPVAAALLDTIARFD
                     LPKAPLLELIQARLRDIYGNRLETVSALESYTEVTCANLFRLATLVLDGDEAVASLGV
                     SGHAGIAYGITGLMRSLPWHSAHGALFVPAEILQAHGVQPDEFAALRTSSALLAALAD
                     LRNLARRHLDIFGPQLHSLPEKSQPAFLPICLCEPYLQLMEKPGYDPFRTIVELPQWR
                     RQWILWRASRRWG"
     gene            4435..5616
                     /gene="bioF"
                     /locus_tag="VNY06_03030"
     CDS             4435..5616
                     /gene="bioF"
                     /locus_tag="VNY06_03030"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015932723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008710 - 8-amino-7-oxononanoate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HWX13836.1"
                     /translation="MQSLDDFAAHKLEELERASLRRGLFDSLREEGTLVTRDGRRLLS
                     FSCNDYLNLTHHPALKAAAIAAIERYGAGAGASRLVTGNHPLFGELEARLARLKGTDA
                     ACVFGSGYLANTGIIPVLAGKKDLILIDELAHACLWAGTQLSRAPTLTFRHNDVGHAR
                     SLLHDKRPQSRHALLVTDGVFSMDGDLAPIANLAALCDAYDTWLMTDDAHGLGVIGGG
                     RGSAFAADTRLVPLQMGTLSKAIGGYGGYLCASKPVIDLMKTRARTLIYSTALPPAMA
                     AAAIAALTIIENEPELCAKPLAKAQAFTSHLGLAKAQSPIVPLIVGAPEAALNASRKL
                     AEQGFLVTPIRPPTVPQDTARLRFAFTAMHPDDEIARLAQDVRSLSEFPSPGKIVREG
                     A"
     gene            5617..6294
                     /gene="bioD"
                     /locus_tag="VNY06_03035"
     CDS             5617..6294
                     /gene="bioD"
                     /locus_tag="VNY06_03035"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012591142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HWX13837.1"
                     /translation="MPAIFITGSGTGVGKTFVATGLIAHFRALGRPVSALKPLVSGFD
                     PASPLGSDPAMLLEALERSVTIDELQRISPWRFRAPVSPDIAARAEGRTIDFQEIADF
                     CRAAIAKTVGSLLIEGIGGIMVPLDRRHTVLDLMVALNQPLILVGGSYLGTLSHVLSA
                     QDVILRRGLDLCAVVISTSEGEPPPLCETLVTLGNFVSAPLIGLTRQAAGGRNREAFQ
                     RLAELVV"
     gene            complement(6267..6725)
                     /locus_tag="VNY06_03040"
     CDS             complement(6267..6725)
                     /locus_tag="VNY06_03040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX13838.1"
                     /translation="MQLDKPQHQESRNKPDVDKFRLSRQFFTWICLGLAFLVAAQDAK
                     AGGSPLDTILSTHLFADVPEAKDFVRESRPPPEALDYQPVTGPDREGPKLRNKDELKA
                     LQNELENAAAQNEEIARKRLGFKKPAPVKPANPTKPDNGEESQTTNSARR"
     gene            7030..>7671
                     /locus_tag="VNY06_03045"
     CDS             7030..>7671
                     /locus_tag="VNY06_03045"
                     /inference="COORDINATES: protein motif:HMM:NF018827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I poly(R)-hydroxyalkanoic acid synthase"
                     /protein_id="HWX13839.1"
                     /translation="MSAQETTSLETAIESQLTAPRASAPPPQNEKPAPGNSRSQTASA
                     DHAPASETAGNDTAEAPAAPDFGRLAFNLARFMEQGGRALAAYFDPSRSGASTGVSDQ
                     LTDAYRVLSPISAHWLSDPARALEAQASLAGKFLGLWANSLRRLSGEQEKPVVPLDPT
                     DKRFAAPEWREVPYFDFLRQAHAINSQWAEDLIQRSTDVDPRVREKARFYLRQI"
BASE COUNT         1474 a         2248 c         2187 g         1762 t
ORIGIN      
        1 cctgttggct aagggttcgg tgcaaagttc cggtcgtcaa ttgtgctcgt caatggcgct
       61 ttcgaagaat gaaaaatgtg gctttagagt agtttccaaa tttccggtat atcgaccgcc
      121 atttcccggt tttacagaat cgcccaggct caacctaggc agcaggctat attccggttc
      181 cgcacggcta gatccgcgca gaccgtcgtc tccttcctag gaccaagtgc cattgctttc
      241 gcccgtgccc tggctatatt cgcgcgccgc gcacggccaa gtcatggcgg gccggcgcaa
      301 gttttgaggc gacgaataag gagaagggat ttgttcacac ctgcttttgc ccaggctgcc
      361 gccggaggag cccccggccc gggagacatg attatgcagc tggtgccctt cgccataatt
      421 tttgttatag cctatgttct cgtcattcgg ccgcagcgca agcggtcaaa gcaacaggct
      481 gatctcctaa aaaatgtgcg ccgaggcgac aacattgtca tgagcggcgg gctgatcggt
      541 aaaattacac gcgtcgttga cgacaacgag attgaactcg aaattgctcc gagcgtgcgc
      601 gtacgcgctg cgagggcaat gatttcagaa gtgcgatcga agggcgagcc tgtaaaggat
      661 caaggctgaa gctgcggtcg catttggatt tcgcggtaac tccagcggac ggcgaaagcc
      721 gcgttcgcaa ccccagggga tagtgcatat ctatgctgcg ttttgcgacc tggaagatcg
      781 catccatttt ggcgatgacg ctctttgcgt ttctcctcat cgtgccgagc ttgctcggcc
      841 ctgggcgccg cgatgctttg atttccttca tgccgaaatg gctgccggtc cgtgccatcg
      901 tgctcggact cgatttgcag ggaggatcgc atgttctttt ggaggtcgac aagaattcgg
      961 tcataaaaac ccttgtcgat aatctccgcg acagtgtcag gcgggttctg cgcgaagaga
     1021 aagtgccgat cacaggcggc ataggtgccc tgccgcgtgg ggtgcaagtg cgcatccccg
     1081 acccgtccga gcgtgaaaag gcaatgccta aacttcgggc gctggcagca tccagcggcg
     1141 gtgtcttgtc ggctgccgat cgcgctccga cctttgatgt gaccgagagc gacaacggcc
     1201 tcatccaatt cacggcgacc gaggctggca tcgaaagcaa ggtgcgccgc gccgttgagc
     1261 aatcgatcga agtgctccgc cgtagggttg atgcgctcgg caccacggag ccaaatattc
     1321 agcgccaagg cgatgatcgc gtgctcgtcg aagtgccagg cttgcaggaa acgagcaagc
     1381 tcaaggaaat tctcggaacg accgccaaac ttgagttccg cctcgtcgcc gagccgggcg
     1441 ccgaccccgc cgaggtagaa ctcttggatg aggtggagga aggtaagaaa atcccgatcc
     1501 aaaagcgggt catggtgcag ggcgaggatc tgaccgatgc ccagccgggc ttcgaccccc
     1561 gcacgcaaga gcccgtcgtg aatttccgct tcaacatccg cggcgggcaa agatttggcg
     1621 aggtcaccgc agaaaacgtt ggaaaattgt ttgcgatcat tctcgacggc aaggtcatct
     1681 ctgcaccgcg catcatcagc ccgattaccg gcggttctgg ccaaatctcg gggcacttca
     1741 cggttgagca ggcaaataat cttgccatat tgttgcgcgc cggcgctttg cccgccaagc
     1801 tcaccatcgt cgaggagcgc acggtcggtc cgggtctcgg ccaggattca atcgatgcgg
     1861 gcaagcgtgc agcctatgtc ggtgccgcgt ttgtcgcctt ctatatgctc atcacctatg
     1921 gcatatttgg cgtcttcgcg aacgtcgcgc tcttcgtgca tatctctttc atctttgcag
     1981 ggctcgtgct gcttggggcg accctcactc ttccgggcat cgctggcatt gtcttcacga
     2041 ttggcatggc ggtcgattcc aatgtcttga tctatgaacg cattcgggag gaggcccata
     2101 tggggcgctc gatcctttcc gcgctcgacg ccggattcaa gcgcgcattt gcaacgatcg
     2161 tcgactcgaa cgtcaccatg ttcgtcgccg ccgccatttt gtattttctg ggatcaggac
     2221 cggtgcgcgg ctttgccgtc tccatggcgc taggcattct gacttccatc gtgacagcgg
     2281 taacaatgac aaggatgatg attgcgcttt ggtaccggta tcagcggccg acgaaattgc
     2341 cgatttgacg gttcgcgtgg ctatttggaa ttgggcccta agggagagta tattttatgt
     2401 ggcaacgcac ccttgcgcag aagcgcggcc tggcgaccgc ttcgttaact atagctccat
     2461 ctcggcaaag gccctgaccc aatgaaactt ttgcgcctcg cccccgaaaa taccaaattt
     2521 cctttcatgc gttttcgtcg cgtgagctat cctttttcgg cgctgctctc gattattgcg
     2581 gtcgttcttt ttatcaattt gggcatgaat ttcgggatcg atttttccgg cggcaccttg
     2641 atcgaactgc gcgccaagaa cggcgccgcc gatcttgcaa aactgcgttc cttggccgag
     2701 cacatggatg tcggcggcgt cgaggtgcag ggatttgggg ggccttccga cgtgaccttg
     2761 cggtttggac ttcaggccgg gggcgatgtc acgcaacagg cggctgtcaa tcgcgtcatt
     2821 tcagcgatcg gcagcgacta tgaggtccgg cgcacggaag ttgtcggccc ccgcgtatcc
     2881 ggcgaacttg tacaatctgg tacgcttggg gtggtcatcg cgatcctcgc ggtgctgtgt
     2941 tacctttggt tccggttcga atggcaattc gccgttggag ccatcatcgc gacgatgcat
     3001 gatcttttgc tgaccgtcgg attttttgcc ctgacaaggc ttgaattcaa caccacatct
     3061 attgccgcca ttctcaccat tgtcggctat tccttgaacg aaaccgtcgt ggtgctcgac
     3121 cgcattcggg agatgatgcg caaataccgc aaaatgccaa ccgaccagct gatcgatgtt
     3181 tcggtcaacg cggtcttgcc tcgcaccatc atgaccgcga caacggtgtt tctcgccctt
     3241 ctctcgctcg ctttattcgg cggcgaagtg atccgctcgt tctcgctggc gatgatctgg
     3301 ggcattttcg ttgccactta ttcgtcgatc tttatttgct ccccgatgct gatctacctc
     3361 ggcttgcgcg ccgacaccgt tggtccggcg cctgccgcga cacccacaaa aacgccagcc
     3421 tagatccggg ttcaagcaag caggcttaaa agttcttgtc tgacaagggc tcatgatggc
     3481 cgcggcggac agtctgacta agggttccga ttatgcctat tgcgaggacc tcatccgccg
     3541 caacgatccg gatcgctggc tcgcgagcct tttcgttccc caagaccggc ggcggcatat
     3601 ccatgctctc tacgcattca gccttgaaat cgcccgcgtg agagaggccg tatccgagcc
     3661 cttgcttgga gagatccgtt tccaatggtg gcgtgatgcg cacgaaggaa aaaatgctgg
     3721 ggatgctagg gccaatcctg ttgccgccgc acttctcgat acgatcgcgc gctttgacct
     3781 acccaaagcg ccactgcttg aactgatcca agcgcgcctt cgcgacattt acggcaatcg
     3841 gcttgagacc gttagcgcgc ttgaatccta cacagaagtg acatgcgcaa atctttttcg
     3901 cctcgccacg ctcgtccttg atggcgacga ggcagtcgcg agcctcggcg tttcgggcca
     3961 cgctggcatc gcctatggga tcactgggct gatgcggtct ctgccttggc attctgcgca
     4021 tggcgcgctt ttcgtcccgg ccgagattct tcaggcgcac ggtgttcagc ccgacgaatt
     4081 tgccgccttg cgcacttcct cggctctgct tgccgcactt gcggatttgc gcaaccttgc
     4141 gcgccgccat cttgatattt ttggtccgca gttgcacagc ctcccggaaa aaagccaacc
     4201 cgcctttttg cctatctgcc tgtgcgagcc ttatttgcaa ctgatggaaa aacccggcta
     4261 cgatccattc agaaccattg tcgagctccc gcaatggcgc cgacaatgga ttctttggcg
     4321 tgcctcccgc cgctggggtt agctcccgtc gcagttgcat cgcttgcgta aatgcgggtc
     4381 ctgcaccggg cgaaggccgg cactcttatc gcgagaatga gaactaaaac acctatgcaa
     4441 tccttggatg attttgccgc ccataaattg gaggagctgg agcgcgcctc tttgcggcgc
     4501 ggccttttcg atagcttgcg cgaagaggga actttggtca cacgcgatgg ccgccgcttg
     4561 ctctcctttt cctgcaacga ttatctcaat ctcacccacc atcccgcgct gaaagccgcg
     4621 gccatagcgg caattgagcg ctatggcgcg ggcgcgggcg cttcgcgtct cgtcaccggc
     4681 aatcatccgt tgtttggcga gctcgaggcc cggcttgcgc ggctcaaggg aaccgacgct
     4741 gcctgcgtat ttggatcggg ctaccttgca aataccggca ttattcctgt gcttgcgggc
     4801 aaaaaggatc tcattttgat cgatgagctc gcgcatgcct gcctttgggc tggaacccaa
     4861 ttgtcgcgcg cgccgacctt gaccttccgc cacaatgacg ttggacatgc aagatccttg
     4921 cttcacgaca agcggccgca atcacgccat gcgcttctcg tcaccgacgg ggttttctcc
     4981 atggacggtg atcttgcgcc gatcgcaaac ctcgccgcgc tctgtgacgc ctatgacacc
     5041 tggctcatga ccgacgacgc gcatgggctt ggcgtgatcg gcggcgggcg tggttcggct
     5101 tttgcggctg atactcggct cgtgccattg caaatgggaa cattgtcgaa ggcaatcggc
     5161 ggatatggcg gctatctctg cgcgtcgaaa cccgtgatcg atttgatgaa aacgcgcgcg
     5221 cggactttaa tttattcgac cgctcttcct ccagccatgg cggccgcggc gatcgccgcc
     5281 ttaacgatca tcgaaaacga gccggaactc tgcgcaaaac ctctcgccaa agcacaggcg
     5341 tttacttccc atcttggcct agccaaggcg caaagcccaa ttgttccttt gatcgttggc
     5401 gcgcccgaag cggcgctgaa cgcctcacgc aaattggcgg agcagggatt tttggtaacc
     5461 cccatccgcc ctccgaccgt ccctcaggac acggcacgac tgcgctttgc cttcaccgcc
     5521 atgcatcctg atgacgaaat cgcgcgcctc gcgcaggacg tgcggtcgct cagcgaattt
     5581 ccgtcaccgg gcaaaatcgt gcgtgagggc gcctgagtgc ccgccatctt catcaccggc
     5641 tcgggcaccg gcgttggcaa gacatttgtt gccaccggcc tcatcgctca tttcagggcc
     5701 ctcggcaggc cggtcagcgc cctaaaacct ttggtaagcg gttttgatcc ggcgtcgccc
     5761 ttgggcagcg atccagccat gctgctcgaa gctcttgaac gcagcgtaac gatcgacgaa
     5821 ctccagcgta tttcgccatg gcgctttcgc gcgccggttt cgccagatat tgccgcgcgg
     5881 gccgagggcc gaacaatcga tttccaggaa atcgctgatt tttgtcgcgc ggcgatcgcc
     5941 aagactgttg gcagcttatt gatcgagggc atcggcggga tcatggttcc gctcgaccgg
     6001 cgccacacgg ttctcgattt gatggtggcc ctcaatcagc cattgatcct tgtcggcggc
     6061 agttatctcg ggacgctcag ccacgtcctc tcagcgcaag atgtgatttt gcgccgcgga
     6121 cttgatcttt gcgctgttgt catctccacg agcgagggcg agccgcctcc gctttgcgaa
     6181 actttggtta cgctcggcaa tttcgtcagt gcccctctga ttggcttgac gcggcaagca
     6241 gcggggggac ggaataggga ggcgtttcag cgcctggcag agttggtcgt ttgactttcc
     6301 tcgccattgt ctggttttgt ggggttggct ggtttgacgg gagcaggttt tttgaagccc
     6361 aggcgctttc gcgcaatttc ctcgttctgc gcggccgcgt tttcgagttc gttctgtaat
     6421 gccttaagct cgtctttgtt tctgagcttc ggtccctcac gatcgggacc cgttaccggc
     6481 tgatagtcga gtgcctccgg cggcggccgg gactcgcgca cgaaatcctt tgcctccggc
     6541 acgtcggcga agagatgcgt gctcaagatt gtgtcgaggg gcgagccacc cgctttggca
     6601 tcttgcgcgg caacaagaaa agcaagcccg agacaaatcc aggtgaaaaa ctggcgtgac
     6661 aggcgaaatt tatccacgtc tggtttattc ctgctctctt gatgttgcgg cttgtctagt
     6721 tgcatgccgg tacaatccaa gtttattgtg tcggaaacgc gacgaaaagg cccggcagga
     6781 atggacccgg cagtctcaag ataggctcgc gcagtcaagc gtcaatgccg gtagagcttt
     6841 gccatttaga gcttggtgaa ccaaaagtgg aaaccgattt tgcgtcccga caagcgacaa
     6901 aaccatgagt tagggcacaa ggacgattgc aaagacaaag tcatcttgct caatgtacac
     6961 ttaacatgac gtgcgtgttg acttttgttg ccggaatttc gcaggcaccg gcaaagcgta
     7021 gaagatgaaa tgagcgcgca agaaaccacg agcctggaaa cggcgattga aagccagctc
     7081 accgcgccaa gggcgagtgc gcccccgcct caaaacgaaa aaccggcgcc cggcaattct
     7141 cggagccaaa ctgcgtcggc cgatcatgct ccggcttctg aaacagcggg caatgacaca
     7201 gccgaggctc cggcagcacc agacttcgga cgtcttgcct ttaatttggc gcgcttcatg
     7261 gaacagggtg gccgcgccct tgctgcctat tttgacccgt cgcgaagcgg cgccagcaca
     7321 ggtgtatctg accaactgac cgacgcctac cgtgtgctga gccccatctc cgcgcattgg
     7381 ttgagcgacc cggcgcgggc gcttgaagcg caagcgtccc tagctggcaa attcctcgga
     7441 ttatgggcca atagtttgcg ccgcttgtct ggtgagcagg aaaaaccggt cgtgcccctt
     7501 gaccctacgg acaaaaggtt cgccgctccg gaatggcggg aggttcctta tttcgatttt
     7561 ctgcggcaag cgcacgccat taactcgcag tgggccgaag atctcatcca gcgttccacc
     7621 gatgtcgatc cgcgtgttcg cgaaaaggcg cgattctacc tccggcagat a
//
LOCUS       DATMPU010000132         2624 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_2902399,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000132 DATMPU010000000
VERSION     DATMPU010000132.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 2624)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2624)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2624
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2902399"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1788
                     /gene="glyS"
                     /locus_tag="VNY06_03050"
     CDS             <1..1788
                     /gene="glyS"
                     /locus_tag="VNY06_03050"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012318137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HWX13840.1"
                     /translation="VLAEILPPVIKSFPWPKSMRWGEASARPGSLRWVRPLHAILATF
                     GPETEETNVVPFAVDGVRSGNLTFGHRFMAPGQIKVRRFDDYVSALEKAKVVLDPARR
                     RDIILYDALNLAMAKGLKLVEDEALLLEVAGLVEWPVVLIGEFDKAFLDIPPEVVRTT
                     IRVNQKCFVLEDPESKKLSNTFILVANIEARDSGAAIAKGNARVVQARLADAQFFWHT
                     DLKVKLEGRLDKLNQIIFHERLGSQGARAERLSALARIIAPYAGADILLAERAGRLAK
                     ADLTSEMVGEFPELQGVMGRYYAAAQGEHESVAEAIEDHYKPQGQSDRVPSAPVAISV
                     ALADKLDLLTGFFAIGEKPTGSKDPYALRRAALGIIALALENKLRLPLRTLFAEALAQ
                     ISFVGVSVFASGANELELMGFFSDRLKVYLRERGTRYDLIDAVFALPGQDDLLLIVTR
                     VEALGRFLDTSDGNNLLAGYRRAANILRAEEKTDGPGAFDGACDPKFLREPAETHLWN
                     TIQSVSLGTQHHLALAFERIGHPGFAQDTSFGAAMECLSSLRAPVDAFFDAVTVNAED
                     ALLRLNRLRLLSELRRAMHWAADFSKIAG"
     gene            1941..2273
                     /locus_tag="VNY06_03055"
     CDS             1941..2273
                     /locus_tag="VNY06_03055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007153503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HWX13841.1"
                     /translation="MAQPGGGSPQKANVALFVRLEAKPGKEAEVAEFLKGGLPLVEAE
                     PATIAWFGIRLGPSTFAIFDAFPDEAGRQAHLSGKVAAALMEKAKDLLAEPPSIQKAD
                     VLAAKLPG"
BASE COUNT          548 a          742 c          768 g          566 t
ORIGIN      
        1 gttctggccg agatcttgcc gccagtgatc aaaagctttc cttggccaaa atccatgcgt
       61 tggggcgagg cctcggcgcg gcccggatca ttgcgctggg tgcgtcccct gcatgcgatc
      121 ctcgccactt ttggtccgga gaccgaagag acaaacgttg ttccttttgc cgtcgatggc
      181 gtgcgctccg gaaatcttac ctttgggcac agattcatgg cgcccggcca aatcaaagtc
      241 cgccggttcg atgattatgt ttcggcgctg gaaaaagcca aagtcgtgct cgatccagcg
      301 aggcggcgcg atattattct ttatgatgcg cttaatcttg caatggcgaa gggcctcaag
      361 ctcgtcgagg atgaggccct actgcttgaa gtcgccgggc tcgtcgaatg gccagtggtt
      421 ctgatcggag agtttgacaa ggccttcctt gacattccgc cggaagttgt gaggacaacg
      481 attcgtgtca atcaaaagtg tttcgtgctg gaggatcccg aaagcaagaa actgtccaac
      541 acattcattc ttgtagccaa tattgaggcc cgtgatagcg gcgcggcgat cgcgaaaggc
      601 aatgcgcggg tcgtgcaagc caggctcgcg gacgcccagt ttttttggca caccgatctc
      661 aaagttaaac tcgaagggag attggataag ctcaatcaga tcatctttca tgaaagactc
      721 ggaagccagg gcgcgcgggc agagaggctc tcagcacttg cccgcatcat cgcaccttat
      781 gctggcgccg acatactcct agccgagcgc gcaggtcgcc tcgccaaggc cgatttgaca
      841 tccgaaatgg ttggagagtt tccggaattg cagggggtca tggggcgcta ttatgcggcc
      901 gcgcagggcg agcatgagag cgtggcggag gcgatagagg accattacaa gccacagggc
      961 cagagcgatc gcgtgccttc cgcgcccgtt gcgatcagcg tcgcgctcgc cgacaagctc
     1021 gacctgctaa ccgggttctt tgccattggt gaaaagccga caggaagtaa agacccttac
     1081 gcgctgcgcc gcgcggctct tggcatcata gctctcgccc tcgagaacaa actgcgcctt
     1141 cccttgcgaa cccttttcgc tgaggccttg gcacaaatca gcttcgtggg cgtgagcgta
     1201 ttcgctagtg gcgccaatga actcgagctc atgggctttt ttagcgaccg gctcaaagtt
     1261 taccttcgcg agcgcggtac taggtacgat ctcattgacg ccgtcttcgc cctgccgggt
     1321 caagacgatc tcctacttat tgtcaccaga gtcgaagcgc ttggccgctt ccttgacacg
     1381 agtgacggca acaatcttct tgccggctat cgccgcgccg ccaatatttt gcgcgccgag
     1441 gaaaaaaccg atggtccggg cgcgttcgat ggcgcctgcg atccaaaatt tcttcgagaa
     1501 cccgcggaaa cccatctttg gaacaccatt cagagcgtga gtttaggtac gcagcatcat
     1561 ttggcgctcg ctttcgagcg catcggccat cccggattcg cccaggacac ctcctttggg
     1621 gctgcgatgg aatgtctgtc ctcgctgcgc gcgccggtcg atgcgttttt cgatgctgtg
     1681 acggtgaacg ccgaggatgc tttgctgcgc ctaaaccggc ttcggctctt aagcgaattg
     1741 cggcgcgcga tgcattgggc cgccgatttt tccaagatcg caggatgata tcgctgcagc
     1801 ttgcaagaat aagggaaagg tggcaggcta cgggtataag tgacggtggt ctcaaaaaac
     1861 ttcaatttcc gaaggaggaa actatgcggc gccgtgaatt ccttgccgct atgggaacag
     1921 ctgcggcggt ttcgtccgcc attgctcagc ctggcggcgg cagcccgcaa aaggccaatg
     1981 ttgcgctatt cgtccggctt gaagcgaagc caggaaagga ggccgaggta gcggaatttc
     2041 taaagggcgg tcttccgctt gtcgaagccg aacctgcgac gattgcctgg ttcggcattc
     2101 gtcttggacc ctcaaccttc gcaattttcg acgctttccc cgatgaagcc gggcggcaag
     2161 cgcatttgag cgggaaagtg gccgccgcgc tgatggaaaa agctaaggat ttgctggccg
     2221 aaccgccgtc gatccaaaaa gccgacgttt tggcggcgaa attgcctggt tagcgctctt
     2281 cgcgcggcgc agctcggcat caatggaatt ccgaagcccc tccgcagccc ggatcgccag
     2341 aggctcgcgc gcgccggtca cactcgcagc atgacgaagc tcggccatgc caagcaaata
     2401 atttcaatgc gatcataatt gatttcgatg cagcggataa taacaaacga tttcccccgc
     2461 cagctcgccg cgcgtatttt tcgcccggtc tgctagccga gatgcgtggt catgcagaca
     2521 tcggcacgtg gcagcctgtg aagtctatca acctatgggg ctagtaatat gagcttagta
     2581 actggaacga cacgcgccgg cgaagtcgcc gccgcggacg cccc
//
LOCUS       DATMPU010000133         5584 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_4439680,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000133 DATMPU010000000
VERSION     DATMPU010000133.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 5584)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5584)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5584
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4439680"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(139..880)
                     /locus_tag="VNY06_03060"
                     /pseudo
     CDS             complement(139..880)
                     /locus_tag="VNY06_03060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018390305.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
     gene            complement(923..2407)
                     /gene="purF"
                     /locus_tag="VNY06_03065"
     CDS             complement(923..2407)
                     /gene="purF"
                     /locus_tag="VNY06_03065"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011664748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HWX13842.1"
                     /translation="MGSLEHGDLEPDASEIDLDEGRLREKCGVFGIFGHPDAAAITAL
                     GLHALQHRGQEAAGIVTFDGHRFNSERRLGLVGDHFSKGPTIERLPGESAIGHVRYST
                     TGETILRNVQPLFAEINAGGFAIAHNGNLTNGLALRRDLVQSGAIYQSTSDTEAILHL
                     VARSRRPHLVDRFVEALRSVEGAYSLVALSNKKLIGARDPLGIRPLVLGELDGQYILA
                     SETCALDIIGAHFLRDIGNGEIAVISDTGIESLRPFPPRPMRPCIFEYIYFARPDSIV
                     HGKPVYNVRKAMGAELAREAPVDADVIVPIPDSGVPAALGFAQHSGIPFELGIIRNHY
                     VGRTFIQPFQSVRELGVRLKHSANRAVVEGKRIVLIDDSIVRGTTSVKIVRMMREAGA
                     REVHFRISSPPIAHPDYYGIDTPDRDRLLAATHTLSEMSSYIGCDSLAFLSVDGIYRA
                     MGEKCRDPDRPQYTDHCFTGDYPTRLTDTGGDTKAQLSLLAEAS"
     gene            complement(2743..3330)
                     /locus_tag="VNY06_03070"
     CDS             complement(2743..3330)
                     /locus_tag="VNY06_03070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012589458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HWX13843.1"
                     /translation="MPSYLDLGLIAVILISAFLAMLRGFTREVLAIISWGAAALAAIY
                     LHPYVLPYVKPYIAKDVFALAASAAAVFFVTLIAVTLVTIKLSDIILDSKIGALDRSL
                     GFAFGAARGLILCVIAYVFFNWLVPVQTQPEWVKSARMRPLLQVTGDQLMAVLPEDPE
                     GLLNKLKKGKSPGLTGEPSPDTGQEPKRAEPGKGT"
     gene            4140..4826
                     /locus_tag="VNY06_03075"
     CDS             4140..4826
                     /locus_tag="VNY06_03075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012592277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HWX13844.1"
                     /translation="MHSLAHVPFFKDAADLDFTRFDRRCTWRQYSGEEIVVDYEDGST
                     DVYFIVSGEVRVLIRTAAGKEVILGEIKAGQFFGEMAAIDGAKRSANVTALTNSEVCI
                     MPTSVFREMIFSSQTICERVLRLLTGRVRELNARITEHSIFDLKHRLYSELLRMARPR
                     QGRADQRAVTPPPLHHVIAARIGCRREQVTRELSALADEGLIEKTRGALVLLRPHVLE
                     TRLDEAMRGC"
     gene            complement(4942..5265)
                     /locus_tag="VNY06_03080"
     CDS             complement(4942..5265)
                     /locus_tag="VNY06_03080"
                     /inference="COORDINATES: protein motif:HMM:NF018460.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HWX13845.1"
                     /translation="MTDILSLFDAAPYRLIAGALTGVILGSFATMLSYRVPRRVSIIW
                     PGSYCPACKTRLQPRDLVPLLSFAAQRGRCRFCGNFIGWRYVVIETALSLAGAGAFVF
                     FGSGF"
BASE COUNT         1244 a         1599 c         1623 g         1118 t
ORIGIN      
        1 agaggtgatc aaagatcaag cggcgggccg cgcgcgccaa ggtaaattct gtgcgtccgg
       61 agactcgaaa gcttgctttc cggcagccag accaacagag acatagcacg cggagcgcta
      121 tttgcgacga ggatcgtttc aagaaggcgg catgaagctc accacccggt cggctctgaa
      181 atcataaagc ttacctgtct cttgccactc cggcatgcac agggcgatga ttttgggcgc
      241 aaagtcttcc ggtgccatta gacttaatgg atcctcgccc ggcattgcag ccgcccgcag
      301 acgggttccc aatggccccg gatcgacgac catcaccgta attttggaaa tattcattgt
      361 ttccgctgca taggtgcggg caagggtgtc gagcgcggcc ttagtaatcg cgtaagcccc
      421 ccaatatggt tttagttgcg cggcatgccg acgtaatgac gatcacccgc ccggcttcgg
      481 cgcggcgcaa aggaacatcg agcgaccgga tcaatcgcca attggcggtc aggttgaccg
      541 ctaaagcttc ggcccacctt gttgtttcga tatgggcaag cggcgtcatc gggccgagca
      601 cgcccgcatt ggcgacaaat atttccagcc ttccccagcg ctcgaaaatg gcagccccga
      661 ggcggtcgag tgcatcaaaa tccttgaggt cgcaaggcac cagcgtcgct tcgctacccg
      721 cgatgcggat ggcgtcgtcg agttcttcga gcgcgccttg ggtgcgtgca agggcaatca
      781 catgcgcgcc cgcgcgggca agctctagag ccaccgcgcg gccaataccg cgcgacgccc
      841 cggtcaccaa agcgatacgg ccgtcaagcg ggcggggcat gagggggttt atagtttatg
      901 ctgggcgggc caagcgccat tcctaacttg cctcggcaag cagcgagagt tgggccttag
      961 tgtcgccgcc ggtgtcggtg agccttgtcg gatagtctcc cgtaaagcaa tggtcggtat
     1021 attgtggtcg atcaggatcg cggcatttct cgcccatcgc tcggtagatg ccatcgacag
     1081 acagaaaggc gagggaatcg caaccaatat atgaactcat ttcactgagc gtatgggtcg
     1141 cggcgagcaa tctgtcgcga tccggcgtgt cgatcccgta ataatcgggg tgggcaatcg
     1201 gcggcgagga aatccggaaa tgcacctccc gtgcgccagc ctcccgcatc atgcggacga
     1261 ttttgaccga agtcgtcccg cgcacgatcg aatcgtcgat gagcacgatc cgcttgccct
     1321 cgacgactgc gcgattggcg ctgtgtttca agcgcactcc caactcccgg acggattgga
     1381 acggctgaat gaatgtgcgc cccacataat gattgcggat gatgccaagc tcgaagggaa
     1441 tgccggaatg ctgcgcgaag cccagggccg cgggcacgcc ggaatcgggg attggaacga
     1501 tgacatcggc atcgaccggg gcttctcgcg cgagctcggc gcccatcgct ttgcgcacat
     1561 tgtacacggg ctttccatgc acgatcgagt ccggccttgc aaaataaata tattcgaaaa
     1621 tgcaggggcg catcgggcgc ggcggaaacg gcctcaggct ctcaatgccc gtgtcggaga
     1681 tcacagcgat ctcgccattg ccaatatcgc gcaagaaatg cgcgccgatg atatcgagtg
     1741 cgcaggtctc cgaagcaaga atgtattgcc cgtcgagctc gccaagaacg agcggccgga
     1801 ttccgagcgg atcgcgggcc ccaatcagct tcttattgct gagcgcgacg agcgaatagg
     1861 cgccctcgac tgagcgcagc gcctcgacga atcggtcgac aaggtgtggc cgccggctgc
     1921 gggcaacgag atgcagaatt gcctcagtgt cggatgttga ttgatagatt gcgccgctct
     1981 gtacaagatc tcgccgcaga gcgaggccat tggtgagatt gccgttgtgc gcgatagcga
     2041 aaccgcccgc gttgatttcg gcgaaaagcg gctggacatt tcgcaaaatg gtctcgcctg
     2101 tcgttgaata gcgcacatgg ccaatggccg attcgccagg caggcgttcg atcgtcggtc
     2161 ccttggagaa atggtcgcca acaaggccga gccggcgttc ggagttgaag cggtgaccat
     2221 caaaggtcac gatgcccgcc gcctcctggc cgcggtgctg cagagcgtgc agacccagag
     2281 cggtaatggc cgcggcgtcg gggtggccaa aaattccaaa aaccccgcat ttctcccgca
     2341 gacggccctc atcgaggtcg atctcagagg catccggctc aagatcccca tgctcgaggg
     2401 atcccatgcc ggagcctatg ctccttcccg cgagatctgc gtttgccatt ttctgcctcc
     2461 ccgaaactgc gggcatgatc agggataatt acgccccgag tttcgcgata tggatctgca
     2521 attttcggat tatgggccct tttccggccc agaatcgcca cgggaatgtc tcaggaatta
     2581 cataattgac atgccggcgg atgtcagcct cgaatgcgtc gcgaacttaa atctgttgcg
     2641 cctgcgctct ctgagggtgc cagcaaaacg taagcttttc cccaacaatc cgcctgcaaa
     2701 cgctggcccc ttgccgcgac aagaaggatc ggacagccgc gcttaggtgc ccttgcccgg
     2761 ctcggccctt ttgggttcct gcccggtgtc tggcgagggc tcccccgtca acccagggga
     2821 tttccccttt ttaagcttgt ttaacaaacc ttccggatcc tccggcagaa cagccatgag
     2881 ctggtcccct gtcacctgga ggagcggacg catgcgcgcg gattttaccc attccggctg
     2941 ggtttgcacg ggcacgagcc aattgaaaaa cacataggca atgacacata ggatcagtcc
     3001 gcgcgcagcg ccgaatgcaa aaccgagtga acgatccagc gcgccaatct tggaatcgag
     3061 gataatgtcc gacaatttga tcgtcaccag ggttacggcg atcaaggtca caaaaaaaac
     3121 cgctgcggca gaagcggcaa gcgcgaacac atccttcgcg atatacggct ttacataggg
     3181 caggacatag gggtggaggt atatagcggc cagagccgcc gcgccccacg atatgatcgc
     3241 gagcacctcc cgggtaaagc cgcgcaacat agcaagaaag gcggaaatca agatgaccgc
     3301 gatcaatccc aggtcgagat aagatggcat tggtttggcc tctcgcgcct aagcgcaatc
     3361 aaatttgccg ttgggttagg aaagttgccg gcaatataga atagaattct gcggcttttc
     3421 ccttaatatt cccgataatg caagggggag cggtgagatt ccatagattc cgccgtgatt
     3481 gggcggcagc gcgtgacgcg cctaaactgc cgtacgaaac tctcgaccag ctaagaaatc
     3541 attttggtga tttcatcacg cctacaactt tggccgccgg cttaaaaccc tgaggggctg
     3601 gttctacgaa tccaccggca aactccggac aagaaccgag agcgattcac tctcgaactg
     3661 atccatcaaa tgccgggcct aaaaaaccag caaaccacca cattgtgcgt ctgcgcacgt
     3721 cggcggaaac ctatttcgaa tagatatggt ggtgtttcag caccgaaaaa tttgcctgcg
     3781 agcgcgctgc aatcgcgtcg cgctcgcagt gttaacttcc ttcacagggg cgggccacct
     3841 cggttccgcc agccagcttc gccaccatcg cgcccttccc ccatcgtctc ctatcgccga
     3901 tccgccggag cgctcgcgct ttcggcttgt gctcggcgat gagccgcgaa ggctacgctc
     3961 caatcggccg caaatgcgca tggcggttaa cggtagatca gcggccgatc cggcttttgg
     4021 ccttgcagca cgaaacagcc gtttgctttt ctttgacaaa tttcgttaaa ggaattcccg
     4081 tcacacctaa ccaagctttc gcaaatagct tttgcctctt tattaaagaa tgggcggcca
     4141 tgcacagcct cgctcatgtt ccgtttttca aggatgcggc cgatctggat ttcacccggt
     4201 ttgaccgtcg gtgcacatgg cggcaataca gtggagagga gattgtcgtc gattacgagg
     4261 acggttcgac ggacgtctat tttattgttt caggtgaagt cagggttttg attcgtaccg
     4321 ctgctggcaa ggaggtcatt ttgggcgaga tcaaagctgg ccagttcttc ggtgagatgg
     4381 cggcaatcga tggcgcgaaa cggtcggcca atgtgaccgc cctgacaaac tccgaagttt
     4441 gtattatgcc gacaagtgtg ttccgggaaa tgatattctc atcgcaaaca atctgcgagc
     4501 gcgttttgag gcttctgact ggacgtgttc gcgagctcaa tgcgcggatt acggaacatt
     4561 cgattttcga tctaaagcac cggctctact ctgagctttt gcgcatggca cgtcctcgcc
     4621 aaggaagggc ggaccaacgc gccgtcacgc cgccgccgtt gcatcacgtc atagccgcac
     4681 ggattggctg ccggcgcgag caggtgacac gtgaactgtc ggcactggcc gatgaaggac
     4741 tgattgaaaa aacgcgcggc gcgctcgtcc ttttgcggcc acacgtcctg gagacacgcc
     4801 tcgacgaggc gatgcgtggc tgctagccta tgccataatt tgacgcatcg ggctaatgaa
     4861 aaagcctatg cattgtggcg cttgcgtgtt ccagccagat tgcgatcgcc gtaaccgacg
     4921 ctacgattag agtcagcgcg atcaaaagcc agagccgaaa aagacgaagg cgcccgcccc
     4981 tgcgagcgac agcgcggttt cgatcacgac atagcgccag ccgataaagt tgccgcaaaa
     5041 gcggcacctg ccgcgctgtg cggcgaagga cagcaagggc accagatcgc gcggctgaag
     5101 ccgcgtcttg caggccggac aatatgatcc gggccaaatg atcgaaacgc ggcgcggcac
     5161 tcggtaactc aacatcgtcg caaagctacc gaggatgacg ccggtcaaag ctcccgcgat
     5221 cagacggtac ggtgctgcgt cgaaaagcga gaggatgtct gtcatgaatt tccttgacgc
     5281 ttaaaatcat aatgcggact tggccgtgga gcgagcggtt aatccagaag cccggccgcg
     5341 gatcatgcca aatgcttcgc gtgaaaaagt tcctgcgcta gagcgtgtcc aacagaattg
     5401 gaaccggttt gcgcctggac acgcgacaaa acaaagattt agagcagatg acgatttcgg
     5461 gaaaagtcat cttgctctag gaaggccgat cggcgctcac tcaccctccc aaggaaaggt
     5521 ccaggtttcc gtgagcgcgc gcgatccggt gcgcaggaaa gcgcgcaaat cggtcgattg
     5581 acca
//
LOCUS       DATMPU010000134         2529 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_2274543,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000134 DATMPU010000000
VERSION     DATMPU010000134.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 2529)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2529)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2529
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2274543"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            77..910
                     /locus_tag="VNY06_03085"
     CDS             77..910
                     /locus_tag="VNY06_03085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013500811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HWX13846.1"
                     /translation="MAQSPEKLPVVATFSILSDFARNVGGERIEVTALVGPNGDTHVY
                     QPKPADAKELGAARLILVNGLGLEGWIDRLIKASGAKALVVVATKGIKPQQMREEGRI
                     EQDPHAWQSIANAKIYVANIRDALIAVDPDGASAYRANADAYHGKLDALEKEVTGKIA
                     EIPPKQREIITTHDAFGYFGTAYGFRFIAPEGVSTETEASARDVAKIIRQIKAQKIPA
                     VFLENVTDPRLARNIATESGAKIGGTLYSDALSPPDGPAGTYIEMMRNNIRELSAALT
                     Q"
     gene            1039..2067
                     /locus_tag="VNY06_03090"
     CDS             1039..2067
                     /locus_tag="VNY06_03090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020173796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HWX13847.1"
                     /translation="MAEKIPVTVLTGYLGAGKTTLLNRILSEDHGQKFAVIVNEFGDI
                     GIDNDLIVDADEEVFEMNNGCVCCTVRGDLIRIIEGLLKRRGKFDAILVETTGIADPA
                     PVAQTFFVDADVRDAARLDAVVTVADAKWLSAQLKDAPEAKDQIAFADVILLNKIDLV
                     SQDEVSDIEARIRAINPYAKLHKTVYCGLPLDAVLRRNAFDLERVLELEPEFLDGEDR
                     HDPGDHEHEYHGHDRHESHGLKHYHDEEMQAVALSVDGDIDPEKFLPWLQDFVQREGP
                     SLLRSKGILAFKNEPRRFVFQGVHMLLDGDLQREWKPGEKRLSKLVFIGRNLPKDVLK
                     EGFLACAA"
     gene            2288..>2529
                     /locus_tag="VNY06_03095"
     CDS             2288..>2529
                     /locus_tag="VNY06_03095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX13848.1"
                     /translation="MLPEETTAAFVAVSPDGTTQEIADEKSRWIDALVTRDDGAVAWS
                     SGKNVRARDPSGSVRTILAPSSVRGLAFLPKGYRIA"
BASE COUNT          566 a          724 c          721 g          518 t
ORIGIN      
        1 cacacggaga ttcggcatgg cacgttatcg ttatgttata accttattca ttgcttgcgc
       61 gagtgcctgg caggctatgg cacagtcccc cgaaaagctg ccggtcgtcg cgaccttttc
      121 gattctttcc gatttcgccc gcaatgttgg tggcgaacgg atcgaggtca cggcactcgt
      181 tggcccgaat ggtgacactc atgtctatca gccaaaacct gccgacgcca aagagctcgg
      241 cgcggcgcga ctcattttgg taaacggcct aggcctggag ggctggatcg accggctgat
      301 aaaggcgtcg ggcgcgaagg cacttgtcgt cgtcgcgacg aaaggaatta agccgcagca
      361 aatgcgcgag gaagggcgta tcgagcaaga cccccatgcc tggcaatcaa tcgccaatgc
      421 caagatctat gttgccaaca tccgtgatgc cctgatcgct gtcgaccccg acggcgcatc
      481 ggcctatcgc gccaatgcgg atgcctatca cggcaagctc gacgcactcg aaaaagaggt
      541 cacggggaaa attgccgaga ttccccccaa acagcgtgaa atcatcacga cccatgacgc
      601 attcggctat ttcggcacgg cctatggttt ccgcttcatc gcgcccgagg gcgtttcgac
      661 ggagacggag gcaagcgccc gcgacgtcgc caaaatcatc cgtcagatca aggcccaaaa
      721 aatcccggcg gtgtttctcg aaaatgtgac cgacccgaga ctcgcgcgaa acatcgccac
      781 agaatccggg gcaaaaatcg gcggcacgct ttattcggac gcgctttcgc ctccggacgg
      841 accggccgga acctatatcg agatgatgcg caacaatatc agggaactct ctgctgcgct
      901 gacgcaatag ggcgaacttg acggcctcca gcaagtttaa gacaaagcaa tagaaaagcc
      961 ttctcgattg cggcctcacc cgcgcgttct tggaagaagg gcgttgctcg atttgctgcc
     1021 aagccaccgg agttccgcat ggccgaaaaa attcctgtaa ccgtgctcac tggttatctt
     1081 ggcgcgggca agacgacgct tctcaatcgc attctctccg aggaccatgg gcagaaattc
     1141 gcggttatcg ttaatgagtt cggcgacatc ggcattgaca atgatcttat cgtcgatgcc
     1201 gatgaggaag tgtttgaaat gaacaatggc tgcgtctgtt gcaccgtgcg cggcgatctt
     1261 atccggatca tcgaaggtct gctcaagcgc cggggcaaat ttgacgcaat cctcgtcgag
     1321 accacaggga ttgccgaccc tgccccggtg gcgcagactt ttttcgtcga tgccgatgtg
     1381 cgcgacgccg caaggctcga tgcagtcgtt accgttgcgg atgccaaatg gctcagcgcc
     1441 cagctcaaag atgcacctga ggccaaggat cagatcgcat tcgccgacgt gatccttctg
     1501 aacaaaatcg atctcgtcag ccaggacgaa gtcagcgaca ttgaggcgcg catccgggcg
     1561 ataaacccct atgccaagct gcacaagacc gtgtattgtg gcctgccact ggacgcggtg
     1621 ctccgccgca acgcttttga cttggagcgc gtcctcgaat tagagcctga atttcttgat
     1681 ggcgaggacc gtcatgatcc cggcgatcat gagcatgagt atcatgggca tgatcgccac
     1741 gagtcgcatg gcctaaagca ctatcatgac gaagagatgc aagcggtggc gctatccgtc
     1801 gatggcgaca ttgatccaga aaaattcctg ccctggctcc aagactttgt acaaagggag
     1861 ggaccttccc ttctgcggtc gaagggcata ctcgctttta agaacgagcc aaggcgattt
     1921 gtgttccaag gggttcacat gctccttgat ggcgatctcc agcgcgagtg gaagccgggg
     1981 gaaaagcggc tgagcaaact cgtcttcatc ggccgtaact tgcctaagga cgtgctcaaa
     2041 gaggggttct tggcctgcgc ggcataacgc gcgtaaacct gaaaagtcat gaccgctgct
     2101 gctctcaccg ccaatgtcat tccatttgat gctggcgcat ctgtcgccgc ggccggcttt
     2161 cttgagcaca ctccggcatt tgccctcgac gatggcaatg tgctgctcag aacggctcag
     2221 ggcccggtga gcgtcgcagc gcatcccggc gcttccataa ttgttgccgc tgtttccgga
     2281 aaacaatttg ttaccggagg agacgacggc cgcgttcgtt gcggtctccc cggatggcac
     2341 gacgcaggag atcgcagatg aaaaaagcag atggatcgat gcgctcgtca caagggacga
     2401 tggtgcggtc gcttggtcga gcggcaaaaa tgtgcgtgcc cgcgatccgt ccggaagcgt
     2461 aaggaccatc ctggcaccct caagcgtgcg tggccttgcc ttcctgccga aaggctaccg
     2521 gatcgcatt
//
LOCUS       DATMPU010000135         6104 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_4441664,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000135 DATMPU010000000
VERSION     DATMPU010000135.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 6104)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6104)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6104
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4441664"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..330)
                     /locus_tag="VNY06_03100"
     CDS             complement(<1..330)
                     /locus_tag="VNY06_03100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HWX13849.1"
                     /translation="MADLLPRFLSAIVLMIAALLALWLGGVVFDLIWLFAALAVGYEW
                     QNLISAPRPRLRSLLAALGLVLGALFSAEVWLGSACASLGVAGVVVALVAGRGRRMWA
                     FCGIVYAA"
     gene            complement(427..1215)
                     /locus_tag="VNY06_03105"
     CDS             complement(427..1215)
                     /locus_tag="VNY06_03105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012592783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HWX13850.1"
                     /translation="MAMDETARSARQRQPRAPIHVGIIMDGNGRWAARQGLPRVEGHR
                     RGVQALRETVRAAIEFGLDYLTVYSFSQENWNRPVKEVADLMGLLKRFIHNDLNEMQD
                     AGVRVRVIGARSNLKADIRTLLEEAEERTKDNTRLNLIVAFNYGSRQEIAAAARILAQ
                     EAAEGLIKPSEIDEALFARRLETAGIPDPDLIIRTSGEQRLSNFMLWQAAYTELVFLP
                     VHWPDFNRQAFAAALKEFSARERRFGGVTGQYRSENLLTSVAKT"
     gene            complement(1445..2005)
                     /gene="frr"
                     /locus_tag="VNY06_03110"
     CDS             complement(1445..2005)
                     /gene="frr"
                     /locus_tag="VNY06_03110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018407583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HWX13851.1"
                     /translation="MANEFDISELRRRMQGAVAALKQELRGLRTGRASASLIEPVHVD
                     AYGQSMPINQVATISVPEPRMISIQVWDKAMVAAVDKAIRNSNLGLSPIVEGQVMRIR
                     IPELNEQRRKEMAKVAHKYAEEARIAIRHVRRDGLDTLKKLLKDKSLSEDDEKRQGHE
                     IQKITDEFVDEIDSALNAKESEIMQV"
     gene            complement(2079..2825)
                     /gene="pyrH"
                     /locus_tag="VNY06_03115"
     CDS             complement(2079..2825)
                     /gene="pyrH"
                     /locus_tag="VNY06_03115"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012592781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HWX13852.1"
                     /translation="MNNSSENKFARKPLAWKRIVVKLSGEALMGRLSHGLDHDVLTSI
                     AADLKDVAARGVEVAVVVGGGNFFRGIEGADRGIERARADSIGMLATVMNALALEYAI
                     EKQGQAARTMSALPMPALCEAYSHQAALHHLAKGRTVVLGGGTGNPFFTTDTGAALRG
                     AELSCDLIMKATKVDGVYSADPKRDPRAIRYDQLTHDEAISRNLAVMDTAAFALAREN
                     GIPIIVFSIREKGAILSALEGRGNATLVLP"
     gene            3359..4219
                     /locus_tag="VNY06_03120"
     CDS             3359..4219
                     /locus_tag="VNY06_03120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018405891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent
                     methylenetetrahydromethanopterin/methylenetetrahydrofolate
                     dehydrogenase"
                     /protein_id="HWX13853.1"
                     /translation="MTKKLLFLLDTDPVPSVFDTVVGYDGGADHVIGYGGVTPENAAA
                     LVDGTIYTRGPKEKQNTAIFVGGGKMTAGEAVFEAVKKRFFANFRVSVMLDSNGSNTT
                     AAAGVALLAKAKPLRAKKAVVLAGTGPVGMRAAAMLHIEGAEVAITSRTKQRAIAASK
                     AIEDRFGFAPVPIEADDNDSRAAAVKGVEIVFAAGAIGVPLLDEKVWQNDSVIELIAD
                     CNAQPALGISGIEAIDKAKERHGKIVIGALGLGGLKLKLHRVCIGQLFESSDQVFDAE
                     NIYAQAKILA"
     gene            4245..4874
                     /locus_tag="VNY06_03125"
     CDS             4245..4874
                     /locus_tag="VNY06_03125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018405890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclodeaminase/cyclohydrolase family protein"
                     /protein_id="HWX13854.1"
                     /translation="MTTKSSIATFLDDLASECPTPGGGGAAAVCGAIGAALVSMVAKL
                     TIGKKNYEAVWEDLKIVNTEAEALRAELTRAIEEDVVAFNSVMGAYALPRATDEEKAK
                     RTAAIQGALKDATLAPLRAVKACFGVIRLSASVAEKGNLNVISDAGVAVLAANAGLRS
                     AALNVFINAKAIKDRDFAEKQIAEVNALIAQATQMTESVYQTVRTKIGS"
     gene            4964..5947
                     /locus_tag="VNY06_03130"
     CDS             4964..5947
                     /locus_tag="VNY06_03130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018484858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HWX13855.1"
                     /translation="MLAFDSRRAALAFAAALISSTALAIAAPASARAAMQAVAPTVHY
                     HTVKVDGIDIFYREAGPANAPVVLLLHGFPTSSHMFRNLIPALADRYHVIAPDYPGFG
                     ESAAPDHMKFAYTFAHYADLVDGLLGQLGAKQYAMYVMDYGAPIGYRLALKHPERVTA
                     LIVQNGNAYEEGLKAFWDPIKAYWTDDSEAHREALSVLVQPTTTKFQYIDGVEDISRI
                     DPDNWGHDQALLDRPGNKDIQLDLFHDYSTNVPLYPQFQAFFRDRKPPTLIVWGKNDK
                     IFPADGAGPYLRDLPDAELHLLDTGHFALEDKLDVMAPLIHDFLDRKVGQR"
BASE COUNT         1282 a         1782 c         1750 g         1290 t
ORIGIN      
        1 agccgcatag actattccgc aaaatgccca catcctccgg cctctgcctg caaccagagc
       61 gacgacgact ccggcgacac cgaggctagc gcaagctgag ccgagccaga cctcggctga
      121 aaacagcgcg ccgagaacga gacccagagc agcgagcagt gagcgcaagc gagggcgcgg
      181 tgccgatatc agattctgcc attcgtagcc gacagccaga gcggcgaaga gccaaatcag
      241 gtcaaagacg acgccgccca gccacagcgc gaggagggcc gcgatcatca aaacaatggc
      301 cgaaaggaaa cgagggagaa ggtctgccaa gccgctccgc cgcaagaaat caagccgcgg
      361 agggtcggtc ggcggcgcgc ccgaggcatc gttccgcata gaaatcagca tccggcgcag
      421 gaaaaatcat gtcttagcga cactcgtcag cagattttca ctacgatatt gccctgtcac
      481 gccaccaaag cgcctttcgc gcgctgaaaa ttctttcagt gccgctgcga aagcttggcg
      541 gttgaagtcc ggccagtgca ccggcaggaa aaccagttcg gtataggccg cttgccacag
      601 catgaaatta gacagtctct gttcgccgga ggtgcggata atgagatctg gatcggggat
      661 gccggccgtt tcgagacggc gggcgaataa cgcctcgtct atctccgatg gtttgatgag
      721 gccctctgct gcctcttggg ccaaaatcct tgccgcggcg gcgatctcct gcctgctccc
      781 atagttgaag gccacgatta agttgagccg tgtattgtcc ttcgtgcgct cctctgcttc
      841 ctctagcagg gtgcggatgt cggctttcaa attgctgcgg gcgccaatga ccctgaccct
      901 tacgccggcg tcctgcattt cattgagatc gttgtggata aagcgcttaa gaagccccat
      961 gagatcggcg acttccttga ccggccggtt ccagttctct tgggagaagc tgtagaccgt
     1021 gagataatcg aggccgaact caatcgcggc gcggacggtc tcgcgaagcg cctgtacgcc
     1081 gcgccggtgt ccttcgaccc gcggcaatcc ctggcgcgca gcccatctcc cattgccgtc
     1141 catgataatg ccaacatgaa tgggtgcacg aggttggcgc tgtcgcgcgg aacgcgcagt
     1201 ttcatccata gccatggatg gcatcgtcat ttgttggcca tcagccgtct ctgacatcgt
     1261 ccctggcaat tccttcgccc agtgcaagac taaagagccg ccacagattc gcttaagtta
     1321 gtcttggggc gacattcccc gggggacccg taataacaga attggttagc ccgtcagcaa
     1381 taatctcggt caggcattag attgaaagcg caagccgagc gtacttagaa cagatcgagg
     1441 caacctaaac ttgcatgatc tcagattcct tagcatttag cgcggaatca atttcatcga
     1501 cgaattcatc ggtaattttc tggatttcat gtccctggcg tttctcgtcg tcctcgctga
     1561 gcgacttatc ttttaggagc ttctttaacg tatctaaccc gtcgcggcgg acatggcgga
     1621 tcgctatgcg cgcctcctcg gcgtatttat gcgcgacctt ggccatttcc ttgcgccgct
     1681 gctcattaag ttccggaata cggattcgca taacctgacc ttcgacaatc ggggaaagcc
     1741 ccaaattgga attgcggata gccttgtcga cggcggcgac catagctttg tcccacacct
     1801 gaatcgaaat catgcgcggt tccggcacgc tgattgtcgc aacttggtta atgggcatgg
     1861 actgcccgta agcatcgaca tgcacgggct caatcaggct tgccgacgcg cggccagtcc
     1921 ttagaccgcg caattcttgt ttcaaagcgg ctactgcgcc ttgcatgcgc cgccttagct
     1981 cggagatatc aaattcattg gccatcttgg tacccttgta tgtttccccc gcgccccaat
     2041 caaggttgtc gtgcccgaaa acgcgacaca ctgggaaatt atggcaagac cagagtggcg
     2101 ttgcctcgtc cctcgagagc agaaaggatc gcgccttttt ctcttattga aaagacgatt
     2161 atgggaattc cattttcgcg cgcaagcgcg aaggcggccg tatccatgac cgcgagattt
     2221 ctcgaaatgg cctcgtcatg cgttaactgg tcatagcgga ttgccctagg gtcacgcttc
     2281 gggtccgccg aatatacccc gtcaaccttc gttgccttca tgatgaggtc gcaggaaagt
     2341 tcggcgcccc gcaaggccgc cccagtgtcg gtggtgaaga atggattgcc cgtaccgccg
     2401 ccaaggacca cagtccggcc ttttgcaaga tgatgcaatg ccgcttggtg tgaataggcc
     2461 tcgcaaagcg cgggcatcgg caaggccgac atcgtgcgcg ctgcctggcc ctgtttctcg
     2521 attgcgtatt cgagagcaag cgcgttcatc accgttgcga gcatgccgat ggaatcggct
     2581 ctagcccgct cgatccctct gtctgcacct tcgatgccgc ggaaaaaatt tccgccaccg
     2641 acaacgactg caacctcgac gccgcgcgcc gccacgtcct tcagatcagc cgctatactg
     2701 gtcaatacat cgtggtcaag gccatgactg agccttccca tcaaggcttc gccggaaagt
     2761 ttgacaacga tccgcttcca ggcaagcggc ttacgcgcaa acttgttttc gcttgaattg
     2821 ttcattgctt caatgtggtt tttgctggca aaatcttgag tgaaagagga gttgatacgg
     2881 aacccttcgc gcgccttaga gaagctttgc ttcggccgcc cgcggcagct caatggtcgt
     2941 cctccgacat ccttacaagg gctacgggta aagcaaatgg agaaaagttc aagacttgct
     3001 gaaaatagcc gctgggtcgc ctgttggccg ggccgcgctt cactgatgca acgatggcta
     3061 gaccccttaa tctggctcat ctacccaata atgtttttgt ctatagcatg gttcgcggcg
     3121 cggcgtttgg tttatcctta gccttttata gatgcggtaa agcttggaac tttgcgggtc
     3181 atgggcaacg ccatcatttc tgctctctat cttttttgtg ccgggggccg gtttgacggc
     3241 cttcgacggc gcgcttggtt cgggcaacca cgggcggagc gggcttggca ggaaagccga
     3301 actctgacaa ataggagctt tcgcactgcg cgggacgatc taactcaacg agaccttcat
     3361 gacaaagaag cttttgtttc tgttggacac cgatccggtg ccgagcgtct tcgacacggt
     3421 cgttggctat gacggcggtg ccgatcacgt cataggttat ggcggagtga cgcccgaaaa
     3481 cgctgctgcg ctcgtcgacg gaacgatcta cacgcgcggt cccaaggaaa agcaaaatac
     3541 cgcgattttt gttggcggcg gcaaaatgac tgctggagag gctgtttttg aggcggtcaa
     3601 gaagcgcttt ttcgccaatt tccgcgtgtc cgtgatgttg gatagcaacg gctctaatac
     3661 cacggctgcg gccggtgttg cgctgctcgc caaggcaaag ccgcttagag ccaagaaggc
     3721 agtagtactc gccggcacgg gccctgtggg catgcgtgct gcggcgatgc tccatattga
     3781 aggcgccgag gtcgcgatta cctcgcgcac gaaacagcga gctattgccg cgagcaaagc
     3841 aatcgaagac cgcttcggct ttgcaccggt gccgatcgaa gcagacgaca atgattcccg
     3901 cgcggcagcg gtcaagggcg ttgagatcgt gttcgccgct ggcgccatcg gcgttccatt
     3961 gctcgacgaa aaggtttggc agaacgactc ggttatcgaa ctaattgccg actgcaacgc
     4021 gcagccggcg cttggtatca gcgggatcga ggcgatcgac aaggcaaaag agcggcacgg
     4081 caaaatcgtg atcggcgcgc tggggctcgg cggcctcaag ctgaaactgc atcgcgtttg
     4141 catcggtcag ttgtttgaga gttcggacca ggtgtttgat gccgaaaaca tctatgcgca
     4201 ggccaaaatt ctggcgtgat tctttttttg gggccgcgac aaccatgacc accaaaagtt
     4261 ctatcgcgac ctttcttgac gatcttgcga gcgaatgtcc gacgccgggc ggtggcggcg
     4321 cggctgctgt gtgtggcgct atcggcgcgg cgctagtctc catggtggcc aagctcacca
     4381 tcgggaagaa gaattatgaa gcggtctggg aagacctcaa gattgtgaat accgaggctg
     4441 aggcgctgcg cgctgagctt acccgcgcaa tcgaagagga cgtggtggcg ttcaattccg
     4501 tgatgggcgc gtatgcgctg ccgcgcgcaa ccgacgagga gaaggccaaa cgcaccgcag
     4561 cgattcaagg cgccctgaaa gatgcgacgc tggcgccctt gcgtgccgtc aaggcctgtt
     4621 tcggggtcat ccgcctatcg gcctctgtgg cggaaaaagg caatctcaat gtcatcagcg
     4681 atgctggggt tgcggtgctt gctgcgaacg ccgggctacg cagcgcggca ctgaatgtgt
     4741 tcatcaatgc caaggcgatc aaggatcgtg attttgctga gaagcaaatc gccgaagtca
     4801 atgcgttgat tgcgcaggcg acgcagatga cggaatctgt ttatcagacg gtcagaacca
     4861 agatcggttc gtgagatcgc gatatcatgg cgtaaacgcg ttaagttcaa gaccctaatt
     4921 ccaataaccc cgccccccga acagacaggt gaggagaccc gagatgctcg cttttgattc
     4981 ccgccgcgcc gcgttggcgt tcgccgccgc gctgatttcc tcgaccgcgc tggcgatcgc
     5041 ggctcctgcg agcgcacggg ccgccatgca ggccgtggcg ccgacggtcc actatcacac
     5101 ggtcaaggtc gatggcatcg acatctttta ccgcgaggca ggccccgcga acgcaccggt
     5161 ggtgctcctg ctgcacggct tcccgacctc ttcgcacatg ttccgcaatc tgatcccggc
     5221 cctggccgac cgctaccatg ttatcgcgcc ggactatccg ggatttggcg agagcgcggc
     5281 gccggatcat atgaagttcg cttacacctt cgcccactat gccgacttgg tggatggttt
     5341 gctgggccag ctcggcgcca agcaatacgc gatgtacgtc atggattacg gtgctccgat
     5401 cgggtaccgg ctcgcgctca agcatcccga acgcgtcacc gcgctcatcg tccagaatgg
     5461 caacgcctat gaagaggggc tgaaggcatt ctgggatccg atcaaggcct actggaccga
     5521 cgactcggag gctcaccgcg aggcgctgtc cgtgctcgtc cagccgacca ccaccaaatt
     5581 ccagtacatt gacggcgtcg aggacatctc ccggatcgat ccagacaatt ggggccacga
     5641 ccaggcgctg cttgatcgcc ccggcaataa ggatatccag ctcgacctgt tccacgacta
     5701 tagcaccaac gttccgctct atccgcaatt ccaggccttc ttccgcgacc gcaagccacc
     5761 gacgctgatc gtctggggca agaacgacaa gatcttcccc gccgatggcg cggggcctta
     5821 cctccgcgat ctgcccgacg ccgagttgca tttgctcgac accggacatt tcgcgctgga
     5881 agacaagctc gacgtgatgg cgccgctcat ccatgatttc ctcgaccgca aggttggtca
     5941 acgctgatgc gaagcggctc gggcacttcg atgccccgct gtgacgcgag gagcagcaca
     6001 ccatggacat ccacgaacat ggagcggatc ttcaaggccg cgaagtcgaa cggctatgaa
     6061 gtgttcatct cgccgcgtta cttctatccg accgacaacg gttg
//
LOCUS       DATMPU010000136         5690 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_730005,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000136 DATMPU010000000
VERSION     DATMPU010000136.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 5690)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5690)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5690
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_730005"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(150..1058)
                     /gene="rsmA"
                     /locus_tag="VNY06_03135"
     CDS             complement(150..1058)
                     /gene="rsmA"
                     /locus_tag="VNY06_03135"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020176130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HWX13856.1"
                     /translation="MQISRQRSTAAVQLDGLPPLREVAAAHGLIAKKTLGQNFLFDLN
                     LTRRIARAAGPLAQATIVEVGPGPGGLTRALLAEGARKLIAIERDTRCIPALAEIQSH
                     YPGRVQIVEENALEINFAELVSGEIPGRVCANLPYNVATALLVHWLETEPWPPWFDRL
                     VLMLQREVAERIVSGPAERAAYGRLSVLANWRCTTRILFNVPASAFTPPPKVRSSLVE
                     LVPRVMPLACDRKLLSQLTQAAFGQRRKMLRQSLKSLSGGNAEALLAEANINPELRSE
                     EIEIEEFIALARALKNFRRELGSIRD"
     gene            complement(1078..2145)
                     /gene="pdxA"
                     /locus_tag="VNY06_03140"
     CDS             complement(1078..2145)
                     /gene="pdxA"
                     /locus_tag="VNY06_03140"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003614202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HWX13857.1"
                     /translation="MTQPLAPTNLPLALSQGDPSGIGPELTLKAWLKTHETPDAPLFL
                     TVADPEHLAAVARHFELKVPVKTVTAGEAPQMFRHALPVCALEQSVSGRPGAPDIQDA
                     AGTIAAIETCVRLIRDGEASATVTNPIAKQLFYQANFHHPGHTEFLAELAQRYFGTRA
                     RAIMLIWSPELAVVPATVHVPLARVPALLTRELLVETAIIVNRDLASRFGIRNPRLAF
                     TGLNPHAGESGAMGREEIDVIAPALADLAAAGVSVSGPVAADSLFRPAARKNYDAVIA
                     MYHDQALIPIKTIAFDRAVNVTLGLPFIRTSPDHGTAFDIAEQGIADPASLLAALNLA
                     AKLAAHAARTVAPPQIASSSP"
     gene            complement(2159..3115)
                     /locus_tag="VNY06_03145"
     CDS             complement(2159..3115)
                     /locus_tag="VNY06_03145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012591227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX13858.1"
                     /translation="MDRVQDARRLFAARGVGFWVGFISAITLIAPCLVPAGDLAFAQT
                     IVASINGDPITNIDIDERMKTLRVLRKPATREAAIESLFSDRLKIREAGKYSINPQDS
                     DLSPQVVSTAKEMNLPPEALLAALEHAGVTPDHFKAHFRAELAFGIFVQALNKGVEAS
                     EEQVRAELAKQGGKAASGMEYTVRQVILAVPHSTTPTTLTERAHDAERLREKFLSCES
                     GIPIAEAMTDVTIRDPIVRTAVELNDRLRQLLDSTPTGRLTPPNRTTEGIEMVAVCKK
                     GASKDDSAARAAIAQKLLAAHVTADAERRLKELRAKAVVAKY"
     gene            complement(3209..>5690)
                     /gene="lptD"
                     /locus_tag="VNY06_03150"
     CDS             complement(3209..>5690)
                     /gene="lptD"
                     /locus_tag="VNY06_03150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012383660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0010033 - response to organic substance
                     [Evidence IEA];
                     GO_process: GO:0061024 - membrane organization [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HWX13859.1"
                     /translation="IVEAAELRYDVEKNTVSAEGNARVYYNDKTLEADRVVYDRKSGR
                     VYAEGHAKFTDRDGTVMHGERFDLTDDFRDGFIESLRADTPPKKYINWPPGPLPGGLP
                     ALEPPPPTTGTYQTHLSAPRAERIGGDTTVFDKGIYTACEPCKDNPDKPPLWRVRAKR
                     IIHKNDEKMIYYEDASIEFVGIPLAYAPYLSGPDPSVTRKSGILTPYLAYNNLLGAGI
                     GVPIFWAFAPNYDLTYTPIYYSQQGFFSNAEWRQRFETGEYNIRATGIAEQNPSVFPV
                     SPYGSRDQNLRGSFESKGQINFADEWKFGWEFTLLSDKYYLNDYHVPSQTLSWNYFSE
                     TTSTAYVTGQGDRGFFDLRGYTFEGLSSHDLQVQQPIAHPLWDYNKTFDIDPAKSFGI
                     GGQAELDFNLTSLSASSASFQAVGPRDLDHAFGLYDICINYIPGRLYGDCLLRGLGGD
                     YTRATAEASWQRKYIDPIGEVFTPFAFARLNGEWLDINTSNIYTFSSAAGSSTYLNQS
                     QQNFLGTKDASLFGAFTPGVGIDYRYPFLAKTSFASMVFEPIGQIILRPNKQIGSNSL
                     VNLDAQSLVFDDSTLFDWSKYSGYDRFETGVRANYGAQLTASFNDGGYINLIGGQSYQ
                     VAGTNSYATPDAANVGLSSGLDTRRSDYVTALTIVPNPIFSFIAKARFDVDSLAARRL
                     DLLATYNFGALTGAIQFANYEAQPVIGYDVRREGLSISSRYKFNENYYAQGNVTFDMS
                     RHLYPPALIDFTNPGPFAIAAFGAGGGYQDECTNFTVSYTSNYQDYGSGSLVRNQTIL
                     LQLQLRTLGNTKISQTFSNTTSLDGVKY"
BASE COUNT         1263 a         1523 c         1664 g         1240 t
ORIGIN      
        1 atgcttgtgg atgtgcctaa acccaaggcg ggatctggac aggtcgtaat caaggtggat
       61 ggcgccgggg taaatattcc cacgctctcg gcgaagcggc gaagcacgca atccgatcag
      121 accggccgat caaatggatc cttcggcgat tagtctcgaa ttgagcctag ttcgcggcgg
      181 aagtttttaa gcgcgcgggc caaggcgata aattcctcga tttcgatttc ttcggaccgc
      241 aattccggat tgatgttcgc ttcggcgagc aaggcctcgg catttccccc gctgagcgat
      301 ttcagcgatt gccgcagcat cttccgcctt tgtccgaatg ctgcttgcgt caactgcgac
      361 aataacttcc tatcgcaggc tagcggcatc acgcgaggaa cgagctcgac gagcgaggaa
      421 cgaaccttgg gcggtggcgt gaaggccgag gcgggaacat tgaacaaaat acgggtcgtg
      481 cagcgccaat tggccaaaac ggacaagcgc ccatatgcgg cgcgctcggc tgggccggag
      541 acaatgcgct cggccacttc gcgttgaagc atcaaaacga gcctgtcgaa ccaaggcggc
      601 cagggctccg tttcaagcca gtgaacgaga agcgcagtcg cgacattgta gggcaggttg
      661 gcgcaaacac gcccgggaat ttcaccggaa acgagctcag cgaaattgat ctccagcgcg
      721 ttctcttcaa caatttgaac tcggcccgga tagtggcttt ggatttcggc aagcgcggga
      781 atgcagcgcg tgtcgcgctc aattgcgata agttttcgtg cgccttccgc aagaagggcc
      841 cgagtgagcc ctccagggcc cgggccgacc tctacgatgg tcgcttgcgc caacgggcca
      901 gcggcacgcg cgatgcggcg ggtgagatta aggtcaaaga gaaaattctg gccaagagtt
      961 ttcttcgcaa tgagcccatg cgcagcggcg acctcgcgca atggcggcag gccatcgagc
     1021 tgaactgccg ccgtcgaacg ctgccgggag atttgcatga tcagctttga gcagaattta
     1081 tgggctagac gaggcaattt gcggcggtgc aacggttcga gctgcgtggg ccgcgagttt
     1141 cgctgcgagg tttaatgctg caaggaggct tgctggatcg gcaatgcctt gctctgcgat
     1201 gtcgaaggct gtgccgtgat cgggcgatgt gcggatgaag ggcaagccca aggttacatt
     1261 gacagcgcgg tcaaacgcaa tggtcttgat tgggatcaag gcctggtcat ggtacatcgc
     1321 tatcacggcg tcgtaattct tgcgcgcggc tggccggaac aaactatctg ccgcaaccgg
     1381 tccgctcact gaaacaccag cggccgcaag atcggccaga gcgggcgcga tgacatctat
     1441 ttcctcccgt cccatggcgc cgctttctcc tgcgtgcgga ttaagcccag tgaacgccaa
     1501 gcgtgggttc cttatcccaa aacgcgaagc gagatctcga tttacgatga tagctgtctc
     1561 gacgaggagt tcgcgcgtca atagggccgg cacgcgcgcg agcggcacat gaacggtcgc
     1621 gggcacgacc gccagttcgg gcgaccagat gagcatgatc gcccgtgcgc gcgtgccgaa
     1681 gtagcgctgc gccaattcag ccaaaaactc agtgtggccg ggatggtgaa agtttgcctg
     1741 ataaaatagc tgcttggcaa ttggattggt gacggttgcc gaggcctctc cgtcacggat
     1801 aagccgcacg caagtctcga tcgccgctat ggttcctgcg gcatcctgga tgtccggagc
     1861 gcccggtctc ccgctgacag attgctccaa ggcacacacc ggcaaggcgt ggcggaacat
     1921 ctgcggcgcc tcccctgcgg tgacggtctt gaccggaact ttcagctcga agtgcctggc
     1981 gactgcagcg agatgctctg gatcggcaac agtaaggaaa agcggcgcat ccggcgtttc
     2041 gtgcgtcttg agccatgcct tcaacgtaag ttcaggaccg attccggagg gatcgccctg
     2101 ggagagcgcc agcggaaggt tggttggggc caatggttgg gtcatcgaga tcttttgatc
     2161 agtacttcgc aactacggct ttcgcgcgca attccttgag ccggcgctcc gcgtcggctg
     2221 tgacatgggc cgccagaagc ttttgagcga tggccgccct tgccgcgcta tcgtccttgg
     2281 acgcgccttt tttgcacacc gcgaccattt cgattccctc ggtcgttcgg ttcggcggcg
     2341 tgagacggcc ggttggggtt gaatcaagca attggcgtag cctatcgttg agttcgactg
     2401 cagtcctgac gatcgggtcg cgaatcgtca cgtcggtcat tgcctcggca atgggtatgc
     2461 cgctttcgca gctcaagaac ttctcacgca agcgttcggc atcatgcgcg cgctccgtca
     2521 gggtcgtggg agtcgtcgaa tggggcaccg ctaaaataac ctggcgcacc gtatattcca
     2581 ttccggaggc cgctttgccg ccttgcttgg caagttcggc gcggacctgc tcctcgcttg
     2641 cctcgacgcc tttgtttagg gcctgcacga agataccgaa ggcgagctct gcgcgaaaat
     2701 gcgccttgaa atgatcaggc gtcacgccgg catgctccag tgctgcgagc aaggcctccg
     2761 gagggagatt catttccttg gcggtactga caacttgcgg ggaaagatcg ctatcttgcg
     2821 ggttaatgct atattttccc gcctcgcgga tcttcagtcg atcagaaaag agactttcaa
     2881 tcgcagcctc gcgtgtcgcc ggtttgcgca agacacgaag cgtcttcatt cgttcgtcaa
     2941 tatcaatatt tgtgattgga tcgccattga tgctcgccac gattgtttgg gcgaaggcaa
     3001 gatccccggc gggaacaagg caaggcgcga taagagtgat ggctgagatg aatcctaccc
     3061 aaaaaccgac gccgcgtgcg gcaaaaagcc tgcgtgcatc ttgtaccctg tccattgccc
     3121 agttgtctcc tcctacgggt gggcctatgc cgagcctctc cctccaggct tcattcccgc
     3181 cggatactgc cttgcgggag taaagaattc aatatttcac accgtcgagg gacgtggtgt
     3241 ttgagaaggt ttggcttatc ttcgtgtttc caagtgtgcg cagctgaagt tgcagcaata
     3301 tggtttggtt gcgtaccaag gatccgctgc cgtaatcttg atagttcgaa gtgtaactga
     3361 ccgtgaagtt tgtgcactca tcctgatagc cgccgcccgc accgaaagcc gcgatcgcaa
     3421 aggggccagg attggtaaaa tcaattaaag ctggggggta taggtggcgg ctcatatcga
     3481 aagttacgtt tccctgcgcg taataattct cgttgaactt gtagcgtgag gaaattgaca
     3541 ggccttcgcg gcgaacgtca tagccgatga ccggttgagc ctcataattc gcgaactgaa
     3601 ttgcacctgt cagcgcaccg aaattatatg tcgccaggag atcgagccgc cttgccgcca
     3661 aggaatcgac atcgaaccgc gccttggcga taaaagagaa aatcgggttg ggcacgatgg
     3721 tgagcgcggt aacataatcg gagcgcctcg tgtcgagccc ggagctcaga ccaacattgg
     3781 cggcgtctgg ggtcgcataa gaatttgttc ccgcgacctg ataggattgc ccccctatga
     3841 ggttgatata gccgccatca ttgaaactcg ccgttagctg cgcaccataa ttggctctga
     3901 cgccggtttc gaatcggtcg taaccagaat atttgctcca atcgaacaat gtggaatcgt
     3961 caaagacaag actctgcgca tccaaattca cgagcgagtt cgaaccaatt tgcttatttg
     4021 gacgcaaaat gatctgtccg atcggttcga acaccatgga ggcgaagctc gtcttggcaa
     4081 ggaacggata gcgatagtca atgccgaccc ccggggtgaa tgcgccaaat aaactcgcgt
     4141 ctttagtgcc aagaaaattc tgctgcgatt gattgagata ggtcgaactg ccagcggccg
     4201 acgaaaaagt gtatatattg gacgtgttga tgtcgagcca ttcaccgttt agcctggcga
     4261 aagcgaaggg ggtaaagact tcgccgatcg gatcgatata tttgcgctgc caggacgcct
     4321 cggcagtagc ccgcgtatag tcgccaccaa ggccgcgcag aaggcaatca ccgtataagc
     4381 ggcccggaat ataattgata caaatgtcgt aaagcccgaa ggcatggtct agatcccgcg
     4441 gtccaacggc ctgaaagctc gcggagcttg ccgaaagact cgtcagattg aaatcaagct
     4501 cggcctgccc gccaatacca aacgatttgg caggatcaat atcgaaggtc ttattatagt
     4561 cccatagcgg atgagcgatt ggctgctgca cttgaaggtc atggctcgac aaaccttcga
     4621 aagtatagcc gcgcaaatca aaaaaaccgc gatctccctg accggtaaca taagccgtcg
     4681 aagtggtttc gctaaaatag ttccacgata gcgtctggct cggtacgtga taatcgttga
     4741 gataatattt gtcggaaaga agcgtaaatt cccagccgaa cttccattca tccgcgaaat
     4801 tgatctggcc cttgctctcg aacgaaccgc gcaagttttg gtcgcgggat ccataaggag
     4861 aaacggggaa gacggacgga ttttgttcgg cgatacctgt cgcccgaata ttatattccc
     4921 cggtttcaaa tcgctgccgc cattcagcat tgctgaaaaa accctgctga gaataataaa
     4981 tgggcgtgta cgtaaggtcg taattgggag caaacgccca gaagatcgga accccgattc
     5041 ctgcccctag cagattgttg taagcgaggt aaggagtaag aatgccggac ttgcgcgtga
     5101 cgcttgggtc ggggccggaa aggtacggcg cataggctag agggatgcca acgaattcga
     5161 tcgaagcatc ctcataatag atcattttct cgtcgttctt atggatgatg cgcttggcgc
     5221 gcacacgcca caagggcggc ttgtcaggat tgtccttaca aggttcgcaa gccgtgtaaa
     5281 tgcccttatc aaagacggtg gtatcgcctc caatacgctc ggcgcgcggc gcgctgagat
     5341 gtgtctggta ggttccggtg gttggtggcg gtggctccaa ggctggcagg ccgccaggca
     5401 atggaccggg cggccagtta atatatttct tgggcggcgt atctgcgcgc aggctctcga
     5461 taaaaccgtc gcggaaatca tcggtgaggt cgaaccgctc gccatgcatc acggtgccat
     5521 cgcggtcggt gaatttcgcg tgaccttcgg catagactct gccactcttg cgatcataaa
     5581 ctacgcggtc cgcttcgagc gtcttgtcgt tgtaataaac ccgcgcattt ccttcggcag
     5641 aaactgtatt tttctcaaca tcatagcgaa gctcagcggc ctcaacaatc
//
LOCUS       DATMPU010000137         6014 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_1973395,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000137 DATMPU010000000
VERSION     DATMPU010000137.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 6014)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6014)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6014
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1973395"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..667)
                     /locus_tag="VNY06_03155"
     CDS             complement(<1..667)
                     /locus_tag="VNY06_03155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018408212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HWX13860.1"
                     /translation="MSIEAHVPFCPVTGRPAVRQIQWVSAGLLRDLWRIEFKTDARAA
                     FRGIDGFGLWESPTGLYFFDPPLEGGHDFYAQFYSKLTGLGLYSQTYIRHEFTMAARS
                     IPAGARVLDVGSGLANFRSCLPKANYTGLDPHMADKSKSAEIRCETLAQHLIEHAGTY
                     DAVCAFQVLEHVTAPAILFAEMVQAVRPGGQIFAGVPHVPSAMTRIPNFIINAPPHHL
                     TWWT"
     gene            complement(792..1097)
                     /locus_tag="VNY06_03160"
     CDS             complement(792..1097)
                     /locus_tag="VNY06_03160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012589138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HWX13861.1"
                     /translation="MTRERKIVRVTIKGRVQGVGYRVWTRMEASARGISGWVRNRLNG
                     DVEAVFAGPPDAVEALCAACWRGPSQAGVTRVNVEGAGEAELALSGAVSGFHQIATR"
     gene            complement(1103..2356)
                     /locus_tag="VNY06_03165"
     CDS             complement(1103..2356)
                     /locus_tag="VNY06_03165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014766249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWX13862.1"
                     /translation="MISLLRAFTPNQRHAVVASYLGWTLDAFDFFILVFVLKDVAQEF
                     GTDVTTVSVAITLTLAARPIGALLFGVAADWYGRRITLMIDILLFSALEFATGLAPSL
                     LIFMIVRLAFGVAMGGEWGVGAALAMETIPPRARGFVSGVLQTGYPSGYLLASVVYFA
                     LFGLIGWRGMFMAGALPALLVLYIRRNVEESPVFLSHAARRVDSGLANLPHIKALRAN
                     VGRFVYAVLLMTAFNFLSHGTQDLYPTFLQVQQKFSPHTVGLIAIIYNIGAIIGGLFF
                     GALSQHAGRRRVIVITSLLVLPVIPLWAYGPGPAVLAGGAFIMQFAVQGAWGVVPVHL
                     NELSPAGVRGAFPGFAYQLGNLLASANATIQAGIAEAYGGNYAFALAAVTAIVAATLA
                     ALAYFGIEAHGIAFDPDDASHKKDS"
     gene            complement(2473..2892)
                     /locus_tag="VNY06_03170"
     CDS             complement(2473..2892)
                     /locus_tag="VNY06_03170"
                     /EC_number="1.-.-.-"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016841382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HWX13863.1"
                     /translation="MFFDFLRQKRLAVISTAHPSGTPESALIGFAVTPDLGFVFDTSS
                     TSRKAVNLKARPEAALVIGWDDEITIQVEGVAHQPQGAELDAAKAAYFSVWPLGRDRE
                     SWPNIVYFVVSPRWLRYSSFATGPEIAEFSFGRHVGS"
     gene            complement(3056..3937)
                     /gene="panC"
                     /locus_tag="VNY06_03175"
     CDS             complement(3056..3937)
                     /gene="panC"
                     /locus_tag="VNY06_03175"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013501994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HWX13864.1"
                     /translation="MTQIPIIATSAASSVEIAHDVASLRAKLAEWQRAGDTIALIPTM
                     GALHAGHISLVREAKKRARWAVMSIFVNPAQFAPSEDFGTYPRSFHADRALFAAAQGD
                     LLFAPRVEDVYGEGFSTTINVRGPAAVELEDRFRPTHFAGVAIIVAKLLNQCRADFAI
                     FGEKDFQQLKVVTRMAHDLDFETRILSAPTVREADGLALSSRNVFLSAEERALAPSLY
                     AALCACATDIKNNVPIATALDMARAKLAAAHFAADYFEARHAETLEPIASLGDGPVRL
                     LAAAWLGKTRLIDNIAV"
     gene            4107..>6014
                     /locus_tag="VNY06_03180"
     CDS             4107..>6014
                     /locus_tag="VNY06_03180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012605808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase"
                     /protein_id="HWX13865.1"
                     /translation="MADSISPDVASSALLYHRLPRPGKLEIVATKPLGTQHDLSLAYS
                     PGVAAACLAIAADPGEAASLTLRQNLVAVVTNGTAVLGLGSIGPLAGKPVMEGKAVLF
                     KKFAGIDVFDIEIAENDVERLVDIIVALEPTFGGINLEDVKAPECFEVERKLRARMSI
                     PVFHDDQHGTAIIVGAAVLNAMELTGKKIDTVKIVTSGAGAAALACLDFLVVLGARRE
                     NIFVTDIEGVVYCGRKALMNDYVAVYAKDTDARALRDVIGGADIFLGLSAGGVLTPEM
                     VKTMAERPLVMALANPTPEIEPEEAAAARPDAMICTGRSDYPNQVNNVLCFPFIFRGA
                     LDVAATTINEAMKLAAARALADLAHEPPSDVVARAYGGKARQYGKDSLIPVPFDPRLI
                     LRIAPAVAKAAMDTGIAKRPLADLALYEQTLTQFVFRSGFVMRPVINAAKACPKRVAY
                     ADGEDERVLRTTQTVVEEGIAKPILVGRPDVINARIERFGLSVRPGQDFELIDPSYDQ
                     RFRSYVETCLESAGRRGVTPKAARLLVRTNSTVIAAIAVKRGDADAMICGLDGRFSPH
                     LPYIRDIIGLAPGATNLSAMCLMITGKGAFFIADTHVQRDPVASEIAEMALLCAAHVR
                     RFGIEPKIAFVS"
BASE COUNT         1320 a         1737 c         1759 g         1198 t
ORIGIN      
        1 tggtccacca tgtcaggtga tggggtggcg cgttgatgat aaaattcgga atgcgagtca
       61 tggcagatgg gacatgaggc acgccggcaa atatttggcc gcccggcctc accgcttgca
      121 ccatctcagc gaacagaatc gctggtgcgg tcacatgttc gaggacctgg aaggcgcaca
      181 ccgcgtcata agtccctgca tgctcgataa gatgctgggc aagtgtttcg cagcggattt
      241 cggcggattt gctcttgtcg gccatgtgtg gatcgagccc ggtgtaatta gcttttggca
      301 ggcatgacct gaaatttgcc aacccactac cgacatcaag gacgcgagcg cccgctggaa
      361 tggaccttgc tgccatcgtg aattcgtggc gaatgtatgt ttgcgaatag agacctaggc
      421 ctgtgagttt tgaatagaac tgggcgtaga agtcgtggcc tccttcgagc gggggatcga
      481 agaaatagag acctgttggt gactcccata atccgaatcc atcgatacct cggaaggctg
      541 ctcgagcgtc agtcttgaac tcaatgcgcc aaagatctct gaggagcccc gccgacaccc
      601 attggatctg ccggacggcg ggccgccccg tgaccgggca aaagggtaca tgagcttcaa
      661 tggacatttt ccaacatcgc gagtagcttc gtgaatggcc tgacctattc ataaaaccgg
      721 caggtttgca aactgcccac tcacaggact cgctgctttg cgttatcaag cagactgtgt
      781 gcggccaaaa tttatcgcgt ggcgatttgg tgaaagcctg acactgcgcc cgatagcgcg
      841 agttccgcct cgccagcgcc ctcgacgtta acccgtgtca cgcccgcttg tgatggtccc
      901 cgccagcacg cggcgcaaag cgcttcaacg gcatctggcg gcccggcaaa aaccgcttcg
      961 acatcgccgt tcaaccggtt tctgacccat cccgaaatgc cccgggcgga cgcctccatc
     1021 cttgtccata cgcgatagcc gacgccctga acgcggcctt tgatcgtcac ccggacaatc
     1081 tttcgttccc tagtcacgga aactatgaat cctttttatg gctggcatcg tcaggatcga
     1141 atgcaatgcc atgcgcttcg atgccaaaat aggctagggc ggccaatgtc gccgcgacga
     1201 ttgccgtgac agccgcaagt gcgaaagcat aatttcctcc ataggcctca gcaattccgg
     1261 cctgaatcgt ggcattcgcc gaggccagca aatttccaag ttgataggca aagcccggaa
     1321 aagccccccg cacgccggca ggtgacagct cgttgagatg tactgggacg accccccaag
     1381 ccccctgaac ggcgaattgc atgataaatg cgccacccgc cagcacggcc gggccagggc
     1441 cataggccca tagcggaatg accggcagaa ccaaaagcga agtgatcaca atgacccggc
     1501 gccttccggc atgttgtgag agggcgccaa aaaagagacc gccaatgatc gcaccgatat
     1561 tgtagatgat cgcgatgaga ccgaccgtgt gcggcgagaa cttctgttga acctgcaaga
     1621 acgtcgggta gagatcctga gtcccatggc tcaggaagtt gaatgcggtc atgagcagga
     1681 cggcgtagac gaagcgtccg acattggcgc gaagggcctt gatgtgcggc agattggcaa
     1741 gaccagaatc cacccgccgc gccgcgtgag acaagaagac tggcgattcc tcgacattgc
     1801 ggcggatata gagaacgagc agcgccggaa gggcgccggc catgaacatt ccgcgccagc
     1861 caattagtcc gaaaagagcg aaatagacaa ccgaggccag aagataaccg cttggataac
     1921 cagtctgcag aacacccgac acgaaacccc gtgcgcgcgg cgggatcgtt tccatcgcga
     1981 gcgcggctcc aacaccccat tcgccgccca tcgccacgcc aaaagcgagc ctcacgatca
     2041 tgaaaatgag aaggcttgga gcgagaccgg tggcaaattc gagcgccgaa aaaagcaata
     2101 tatcgatcat gagcgtgatt cggcggccgt accaatcggc cgcgacgcca aagagcaaag
     2161 cgcctatggg ccgtgccgcc aatgtaagtg tgatggctac ggagacggta gtgacatcag
     2221 ttccaaattc ctgcgccacg tccttcagca caaagacgag aatgaagaaa tcgaaagcgt
     2281 cgagcgtcca gccgaggtag ctcgcgacga cagcatggcg ctgattggga gtgaacgccc
     2341 tgagcaagga aattataatc gctcctcaag gaccggagaa ggacaaggga ccggcggaag
     2401 gcagggccaa actcaccata tttttcgctg ctcgtagaga aaaccggtag ggtctcagtt
     2461 tgaatcccgc tctcaactcc cgacgtgacg cccgaagcta aattctgcga tttccggtcc
     2521 ggtcgcaaag ctggagtagc gcaaccatct cggagagacg acgaagtaaa cgatgttcgg
     2581 ccaactttcc cgatctcgtc caagaggcca gacagagaaa tatgctgctt tggctgcgtc
     2641 tagctccgcg ccttgcggct gatgcgcgac gccttctact tggattgtta tttcatcgtc
     2701 ccagccaata accagcgcag cttccgggcg tgccttcagg ttcacggctt tccgtgatgt
     2761 tgaggaagtg tcgaagacga agcccaagtc aggagtgaca gcaaacccaa tgagtgctga
     2821 ctcgggagtt ccagatgggt gggcggttga tatcaccgcc aggcgtttct gacgcagaaa
     2881 gtcgaaaaac atcttcgcat ttcattgaac gccactaccc tgttccgcgt gtttgcctca
     2941 gactcaggca ggttcgaggg cgacttgcaa tggcccaatt ccacccctag ggaaattcaa
     3001 actgagacac aaccgaaaaa cgtgtcttcg gctggcccgc cggtccccct gctggttaaa
     3061 cagcgatatt gtcgatgagc cgcgtcttgc caagccacgc agccgcgagc agccggacgg
     3121 gtccatcgcc gagcgaggcg atgggctcta gcgtttccgc gtgacgcgcc tcaaaataat
     3181 cggcggcgaa atgagccgcg gcgagttttg cgcgggccat atcgagcgct gttgctatag
     3241 gaacgttgtt tttgatgtcg gtagcgcatg cgcaaagcgc cgcataaagg cttggcgcga
     3301 gcgcgcgctc ctctgctgac agaaagacat tgcgtgaaga tagcgcgagg ccgtccgctt
     3361 cgcgcaccgt gggcgcagag agtattctcg tttcgaaatc gagatcgtgc gccatccgcg
     3421 tcacgacttt gagctgctga aagtcctttt cgccaaaaat cgcgaaatcg gcgcggcatt
     3481 gattgagaag ttttgccacg atgatcgcga cgccggcgaa atgcgttggc cggaaacggt
     3541 cctccaattc cacggccgcc gggcccctca cgttgatcgt ggtggaaaat ccctcgccat
     3601 aaacgtcctc gactcgcggc gcgaacagca gatcgccctg cgcggccgcg aataaagccc
     3661 tatccgcgtg aaaggagcgc ggataggttc caaaatcctc cgaaggcgcg aattgagccg
     3721 ggttcacaaa aatcgacatc accgcccagc gcgcgcgttt tttcgcttca cggacaaggg
     3781 aaatatgccc ggcgtgaagc gcgcccatgg ttggaatcag ggcaatggta tcgcccgcgc
     3841 gctgccattc cgcaagcttt gctctgaggc ttgccacgtc atgcgcaatt tcgacgctgc
     3901 ttgctgcgga ggtggctatg atcgggattt gtgtcatcga gtcataacat ctgcggctgc
     3961 cggctcgcgg ccccgtgccg ggccaagcgc gatgcttccc ggccaggaaa cgttaactta
     4021 atggccagga cgctggttca cctgcgtggt ggacatcggc gcgcggtcag gctatctcct
     4081 gcccataatc cagagaaacc aggctcatgg ccgactccat cagtcccgat gtggcgtcga
     4141 gcgcgctgct ctaccatcgc ctgccgcggc ccggcaagct agagatcgtg gcgaccaagc
     4201 cgcttgggac gcaacatgat ctgtcgcttg cttattctcc gggcgttgcc gcggcatgcc
     4261 tcgcgatcgc cgccgatccc ggcgaggctg cgtcgctcac cttacggcaa aatctcgttg
     4321 cggtggtaac gaatggcacg gcggtattgg ggcttggcag cataggtcct ctcgcgggca
     4381 agcccgtgat ggaaggcaag gccgttctat tcaagaaatt cgcaggcatc gatgtattcg
     4441 acatcgagat agcggagaat gacgttgagc gtctggtcga tatcatagtg gcgcttgagc
     4501 caacttttgg cggaatcaat ctggaagatg tgaaagcccc ggaatgtttc gaggtcgagc
     4561 gcaagctgcg cgcgcgaatg tcgattccgg tttttcacga cgatcagcat ggcaccgcga
     4621 tcattgtcgg cgcggcggtc ctcaacgcca tggagcttac cggcaaaaaa attgacaccg
     4681 tcaagattgt gacctcgggc gcgggggcgg cggcgcttgc ctgcctcgat ttcctggttg
     4741 tccttggtgc taggcgcgag aacatttttg ttaccgatat cgaaggtgtc gtctattgcg
     4801 gccgcaaggc cttgatgaat gattatgtgg cggtctatgc caaggacacg gatgcccgcg
     4861 ccctcaggga cgtcatcggc ggcgccgata tttttctcgg gctttcggcg ggaggtgtcc
     4921 tgacgccaga gatggtgaaa acgatggccg aacggccgct cgttatggcc ctcgccaacc
     4981 ccacgcccga aatcgagcct gaagaggcag ctgcagcaag gcccgatgcg atgatctgca
     5041 ccgggcgctc cgattacccc aatcaggtca ataatgtcct ttgcttcccc ttcatctttc
     5101 gtggtgctct cgacgttgcg gcgacgacca tcaacgaagc aatgaagctc gctgcggcgc
     5161 gcgcgctcgc tgatctcgcg cacgaacctc cttccgacgt ggtcgccagg gcctatggcg
     5221 gcaaggcacg gcagtacgga aaggacagtc tcatcccagt gccattcgat ccgcgcttga
     5281 tcctgcgcat cgcgccggca gtcgcaaagg ccgctatgga tacgggcatt gcgaaaagac
     5341 cgcttgcgga tcttgcgctt tatgaacaga ccttgaccca gtttgtgttc cgttccggct
     5401 tcgtcatgag accggtgata aatgctgcca aggcctgccc taagcgcgtg gcttatgcgg
     5461 atggggagga tgaaagggtg cttcgcacga cccaaaccgt ggtcgaggaa gggatagcca
     5521 agcccattct cgtcggccgc ccggacgtga ttaacgcccg catcgaacgg tttgggcttt
     5581 ccgtgcgccc cgggcaagac ttcgagctca ttgatccaag ttatgatcag cgttttcgca
     5641 gctatgtcga aacttgcctt gagtcggccg gcaggcgagg cgtcacacca aaagccgcgc
     5701 gactcctggt acggacgaat tcgaccgtta tcgcggcaat cgcggtcaag cgtggtgacg
     5761 cggacgcaat gatttgcggt cttgatgggc ggttcagccc ccacttgccc tatatcaggg
     5821 acattatcgg gctggctcct ggcgcaacca atctctccgc catgtgcctc atgatcaccg
     5881 gaaagggcgc cttttttatc gccgacacgc atgtgcaacg ggatcctgtg gcttcagaga
     5941 tcgcggaaat ggcgctcctg tgtgccgccc acgtccgccg atttggtatc gagccaaaaa
     6001 tcgcttttgt gtcg
//
LOCUS       DATMPU010000138         5277 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_3520162,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000138 DATMPU010000000
VERSION     DATMPU010000138.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 5277)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5277)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5277
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3520162"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            122..1903
                     /gene="rpsA"
                     /locus_tag="VNY06_03185"
     CDS             122..1903
                     /gene="rpsA"
                     /locus_tag="VNY06_03185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018408883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HWX13866.1"
                     /translation="MASTNTYAPSREDFAALLEESYGESEAIEGSVIKGRVVGIEKDV
                     AVIDLGLKTEGRVPLKEFHGAGREGSVQIGDEVEVYLERIENALGEAVISRDKARREE
                     SWVKLEKAFEKQEKVEGIIFNQVKGGFTVDLDGAVAFLPRSQVDIRPIRDVAPLMQTP
                     QPFQILKMDRRRGNIVVSRRTVLEESRAEQRHEIVANLAEGQVIDGIVKNITDYGAFV
                     DLGGIDGLLHVTDVAWRRVNHPTEVLTIGQTVRVKIIKINHETHRISLGMKQLLDDPW
                     QNIDAKYPVNARFQGRVTNITDYGAFVELEPGIEGLIHVSEMSWTKKNVHPGKIIATS
                     QEVEVQVLEVDPVKRRISLGLKQTLVNPWEAFAAKYPPGTELEGEVKNKTEFGLFIGL
                     DGEVDGMVHLTDLDWNKPGEQVIDNYKKGDTVKAKVLDVDVGKERISLGIKQLTPDPF
                     AVKPAGETGAKASDEDGAGLKKGSVITCEVLAVKEGGIDVKLVGTDLTAFIKRSELAR
                     DRADQRTERFAVGEKVDARITVFDRRARKISVSIKALEVAEEKEAIAQYGSADSGASL
                     GDILGAALKAREDTPKKSRKGKTEETE"
     gene            complement(2095..3198)
                     /gene="hppD"
                     /locus_tag="VNY06_03190"
     CDS             complement(2095..3198)
                     /gene="hppD"
                     /locus_tag="VNY06_03190"
                     /EC_number="1.13.11.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008836695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003868 - 4-hydroxyphenylpyruvate
                     dioxygenase activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylpyruvate dioxygenase"
                     /protein_id="HWX13867.1"
                     /translation="MGPFPHDAPPAKISFENPAGTDGFEFVEFAHHDPNALKALFEQM
                     GFELVARNKYRDIRVYRQGGVNFILNADRQSFGGGFVDPHGPCAPAMAWRVVNAEHAL
                     AHAVERGATPYLGTTGAKTIDAPAILGIGGSLIYFIDIFGAKGSPYDAEFDWVGERDP
                     VPAGHGIDYIDHLTNNVFRGAMDRWYQFYQNLFGFRQIRYFDISGKKTGLYSRALTSP
                     CGKIRIPLNESADDKSQIEEFLHQYKGEGIQHVACGSKDIYATVKWLRANGLAFMPSP
                     PDTYYKRIAARLPGHDEPSELLRDSGILIDGEVVEGMRPKLLLQIFSKTVIGPIFFEF
                     IERKGDEGFGEGNFQALFESIEADQIERGVLRA"
     gene            3307..3807
                     /locus_tag="VNY06_03195"
     CDS             3307..3807
                     /locus_tag="VNY06_03195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012040899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HWX13868.1"
                     /translation="MPESQELGALDQLDLKLLSALEADGRLTNAELGDRIGLSASQCS
                     RRRIRLEEIGIIDGYHARLNSGRLGLGLRAFIQVSLSPHSKENSRLFRDFVNGIEEIQ
                     EAYALTGDADYLLKIVAANLSGLAHVINDLILPHASVAHVKSSIVLDELKDSHRLPLK
                     AAKSRA"
     gene            complement(3808..4490)
                     /locus_tag="VNY06_03200"
                     /pseudo
     CDS             complement(3808..4490)
                     /locus_tag="VNY06_03200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007591838.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
     gene            complement(4522..>5277)
                     /gene="phhA"
                     /locus_tag="VNY06_03205"
     CDS             complement(4522..>5277)
                     /gene="phhA"
                     /locus_tag="VNY06_03205"
                     /EC_number="1.14.16.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004448142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004505 - phenylalanine 4-monooxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine 4-monooxygenase"
                     /protein_id="HWX13869.1"
                     /translation="AETLPSRACDAFFRGLEALDLYGQGIPDFAQLNEKLSALTGWTT
                     VAVPGLVPDGIFFDHLANRRFPAGNFIRGADELDYLKEPDIFHDVFGHVPMLTDPVFA
                     DYMQAYGKGGQRALSLGHLQNLARLYWYTVEFGLLETPSGLQIYGAGIVSSRTESIFA
                     LASPSPNRLGFSLERVMRTLYRIDDFQQVYFVIPSLQCLLDVTTKTDFGPLYTKLSRL
                     PDIPIAAMEPGDRVFTLGTQDHANSGGRLGQGA"
BASE COUNT         1226 a         1441 c         1531 g         1079 t
ORIGIN      
        1 cctttttgcg ccagcccggt ttcgggggtc tgcctgacaa atagcagaaa ttggcggact
       61 ggaagcgcaa ctaactctaa ccaaactggc gccgcccgca tagggacacc caggagaaca
      121 aatggcatct accaacacct acgcgccgtc gcgcgaggat tttgcggctt tgctcgaaga
      181 aagctatggc gagagcgaag cgattgaagg gtcggtcatc aagggtcgag tcgtcgggat
      241 cgagaaggac gttgcggtta tcgatcttgg gctcaagacc gaaggccgcg tgcccctaaa
      301 agaatttcat ggagccgggc gggaaggctc cgtccaaatc ggcgatgagg tcgaagttta
      361 tctcgagcgg atcgagaacg cgctgggcga ggcggtgatc tcacgcgaca aggcgcgccg
      421 ggaggaaagc tgggttaagc tcgaaaaggc tttcgaaaag caggaaaagg ttgagggcat
      481 catcttcaat caggtcaagg gcggcttcac ggtcgatctc gatggcgccg tcgcgttctt
      541 accgcgcagt caggttgata tccgcccgat acgtgatgtg gcgccgctga tgcagacgcc
      601 gcagcccttc caaattctga aaatggaccg ccgccggggc aatatcgtcg tctcgcgccg
      661 taccgttctc gaggaatctc gggccgaaca gcgccacgag atcgtcgcca atctcgcgga
      721 aggtcaggtg attgacggca tcgtcaaaaa cattaccgat tacggcgcct tcgtcgatct
      781 tggcggcatc gacgggcttc tacatgtgac cgatgtcgcc tggcggcggg tcaatcaccc
      841 aaccgaagtt ttgacgatcg gccaaacggt gcgggtaaag atcattaaga tcaatcatga
      901 gacccaccgc atttcactcg ggatgaagca attgctcgac gatccgtggc agaatatcga
      961 cgcgaaatat ccggtcaacg cgcggttcca aggcagagta accaatatca ccgattatgg
     1021 agctttcgtc gagctcgaac ccgggatcga gggcctgatc cacgtgtccg aaatgtcctg
     1081 gaccaagaag aatgtgcatc caggcaagat tattgcgacc agccaggagg tcgaagtcca
     1141 ggtgctcgaa gtcgatccgg tcaagcgccg catctcgctt ggcctcaagc aaacgcttgt
     1201 caatccctgg gaagcctttg ccgcgaaata tccccccggc accgagctcg agggcgaggt
     1261 caagaacaag accgagttcg gacttttcat tggcctcgac ggggaagtgg acggcatggt
     1321 tcatctgacc gatctcgatt ggaacaagcc cggagaacag gtcatcgaca actataaaaa
     1381 gggcgatacc gtcaaagcga aggtgctcga tgtcgatgtc ggaaaggagc gcatctcgct
     1441 tggcatcaaa caattgactc cagatccgtt cgcggtgaag cccgcgggcg aaactggtgc
     1501 caaggcaagc gatgaggatg gcgctgggct taagaagggt tccgttatca cttgcgaggt
     1561 actggcggtg aaggagggcg gaatcgacgt aaaactcgtc gggaccgatt tgaccgcttt
     1621 cattaaacga agcgaactcg cgcgtgaccg cgctgatcag cggacggaac gcttcgctgt
     1681 tggcgagaaa gtcgatgcga ggatcaccgt attcgaccgc cgcgcccgca agatatcggt
     1741 ctccatcaag gcgctggaag tcgccgagga aaaggaagca attgcccaat atggctcggc
     1801 cgattctggc gcgtctcttg gtgatatttt gggcgccgcg ttaaaggcgc gcgaggatac
     1861 gcccaaaaag tcccgcaagg gcaagaccga agaaaccgaa tagagctttg cgcccgtatg
     1921 ggcatcagaa cgaagacggc ggaagcacaa aaaagcgaga agacgatcag catcaaagct
     1981 gcttgaagag gtatcggcgt acggttattt gcttttttgg cgcggtgcta gttatcaggt
     2041 gacgctgagc agctgccgca acggcgcgcg cgatttcgac tcgcaccgga tattttatgc
     2101 cctcagcaca ccacgctcga tctgatccgc ttcgatcgac tcaaagagcg cctggaaatt
     2161 accttccccg aaaccctcat ctcccttgcg ttctataaat tcgaagaaaa tggggccaat
     2221 gacggtcttg gaaaaaattt gcaacaggag ttttggcctc ataccctcaa cgacttcgcc
     2281 gtcaatgaga attccactgt cccttaagag ttcggatggc tcgtcatggc cgggaagccg
     2341 cgcggcaatt ctcttgtagt aagtatcggg tggtgatggc ataaacgcta ggccgttcgc
     2401 ccgtagccat ttcacggtgg cataaatatc cttggatccg caggcaacat gttgaattcc
     2461 ttcgccctta tattgatgca ggaactcctc aatctgactc ttgtcgtcgg cggattcgtt
     2521 caagggaatc cggatctttc cgcacgggct cgtcaaggcg cgcgaatata gcccggtttt
     2581 tttcccggaa atgtcaaaat agcgaatctg acggaaacca aagagatttt gatagaattg
     2641 ataccagcgg tccatggcgc cgcgaaacac attgttggtc aggtgatcga tatagtcgat
     2701 gccgtggcct gctggtacag gatcgcgctc gccgacccaa tcgaattcgg catcataggg
     2761 cgagcccttg gcgccaaaaa tgtctataaa atagatcagg cttccgccaa tcccgaggat
     2821 ggctggagcg tcaattgttt tggcgccggt cgtgcccaaa taaggcgtag cacctcgctc
     2881 gaccgcatgg gcgagagcat gctcggcatt gacgacgcgc caagccatgg cgggtgcgca
     2941 cggaccgtgg ggatcgacaa atcccccgcc aaaactctgc cgatcggcgt taaggatgaa
     3001 gttgaccccg ccttgacgat agacgcggat atctcggtat ttgttccgcg cgacaagctc
     3061 aaaccccatt tgctcgaaca acgccttgag cgcgttgggg tcgtgatggg cgaattccac
     3121 aaattcgaaa ccatcggtcc cggctgggtt ttcgaagctg attttggccg gcggcgcatc
     3181 atgcggaaac gggcccatcg gtctcctcat cgatttttgg ctagttgaaa tatagcgcga
     3241 aacccgccgg agaagtgtgt atagtgacgg acacccccaa aaacaaatgc acgaatcgtg
     3301 tgagaaatgc ccgaatcgca agagttaggc gcgcttgatc aactcgatct caagctcctt
     3361 tcggcgctcg aggccgacgg gcgactaacc aacgccgagc tcggcgacag gatcggcctc
     3421 tccgcgtcgc aatgttcccg tcgccgcata aggcttgagg aaattggcat tatcgacggg
     3481 tatcacgcgc gcctgaatag cggccggctc gggctcggtc ttagggcctt catccaggtc
     3541 agtctgtccc ctcattccaa ggaaaattct cgactattca gggactttgt aaatggaatt
     3601 gaagaaatac aggaggccta tgcgttgacc ggtgacgccg attatctttt gaaaattgtg
     3661 gctgccaatt tgagtgggct cgcccatgtc attaacgatt tgatcctgcc tcacgcgagc
     3721 gtggcgcacg tcaaatcgtc gatcgtgttg gatgagctca aggatagcca ccgcttgccg
     3781 ctcaaggcgg ccaaatcgcg tgcttgatta cgtccgtcca gaaatgagga gttccgcgcc
     3841 ggtgatcgct gcggcgctat cggagagaag aaacaagatg gccttggcaa tatcttccgg
     3901 tttcacccag cgcgaaaaat cagccttcgg catatccgcg cgattgcgcg gcgtatctat
     3961 gattgtcggc agcactgcat tgacgcgggc gccagcgttc ttttcttcct ccgcaaggct
     4021 ttcggtcagc cgcagcacgc cagccttcga ggcggcataa gcgcccattc ccgcgccagt
     4081 ttttttggcg ggtgctgatc ccacgttgac gattgcaccc tgcgtcgcgc gaagataggg
     4141 aagcgccgct cttattgccg tcattgctgt taatagattc atacggaaga gatcggtcca
     4201 gccggaaaga tcgccatcgc cgagcgtctg ccagcggaag ccgcccgcgg cattagcagg
     4261 ccgtcgatct tgccgaaacg cgcggcaacc gcgtcaatgg cttgcttggt ggctgcaaaa
     4321 tctgtcaatt ccacgccgcc gagtaccatt aggcgatccc gcgcaggaat cttgtctgct
     4381 tgtctgcggg cggggatggc gacataggct ccggccgcga gcgccgcttc catcacggca
     4441 cccccgagca cgccaaaacc tccggtgaca actataagct tgtcccgcat gtgctgttcc
     4501 tcctcaacga atggcaagtg attaggcccc ttggcccagg cgtcccccgc tattggcatg
     4561 gtcttgagtg cctagcgtaa aaaccctatc gccaggctcc atcgcggcga ttgggatgtc
     4621 gggcaggcgc gacaatttgg tgtagagagg gccaaaatcg gtcttagttg taacgtcgag
     4681 caaacattgc agcgacggaa tcacgaaata gacctgctgg aaatcgtcga tgcggtagag
     4741 tgtgcgcatc accctttcga ggctgaaccc caagcggttg ggtgaagggg aggcgagggc
     4801 aaaaatcgat tccgttctcg acgacacaat gccagcaccg taaatttgca agccgctcgg
     4861 ggtttccaaa agcccgaatt cgacggtgta ccaatagagg cgggcgaggt tttgcaggtg
     4921 accgagggac agcgcccttt ggccaccctt cccataagct tgcatgtagt cggcgaatac
     4981 gggatcagtc agcatcggca catggccaaa gacatcgtga aaaatatctg gctctttcaa
     5041 atagtcgagc tcatcggcgc cgcggatgaa attcccggca gggaagcgcc ggttcgcgag
     5101 atggtcgaaa aatatgccat cgggcacgag gcccggtacc gcaacggtcg tccatcccgt
     5161 aagcgccgaa agcttttcgt tcaattgcgc aaagtcagga atgccttggc cgtacagatc
     5221 gagcgcctcc aggccgcgaa aaaaagcgtc gcaggcgcgg ctaggcaggg tctccgc
//
LOCUS       DATMPU010000139         7262 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_3522244,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000139 DATMPU010000000
VERSION     DATMPU010000139.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 7262)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7262)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7262
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3522244"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(173..2188)
                     /locus_tag="VNY06_03210"
     CDS             complement(173..2188)
                     /locus_tag="VNY06_03210"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012592260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HWX13870.1"
                     /translation="MSIGGLGQIQTYGVYLQRAQLRGDNEFGLDPAIEAVGPRAATFD
                     RRRVSLRWLSGTLLTGISGAGLIGAAIYAALGHQTYFTELPFPALSQRKEINLDFGVN
                     PRKGDRLVKAVDIGAGKQRFQAQAAIRAGDKEVVKLHTFTHIQTSLTLASLGLSEDVP
                     TYNPLKVLADARAPIDAAQELVQDDAEIAWTTRDLGDQSFSASAALSIEEVQAQVAEQ
                     VKNTMAAGAKPLALPPQLLLMRTSRANLPGVLAYANPSEQITTAPFSTIEVRMVPENV
                     TSVGRSPQAAQVTQMEERLVVVRRGETIENVLRAAGLSKEQVNDVISAFGVTRGDAAV
                     AEGARLKLLFADIDGSGSNMTLARLSVYSGETLETNIAVNDRGGYVRVTPPRHVAKRP
                     SRDESEEADDSDAMRLYDSFYETALKQEIPRPIVDELVRIFANDVDFQRGVSAGDSFE
                     VLYDDSEEGEAHNQLLYAAINARNETYRYYRFQTPDDASIDYYDQDGRSTRKFLVRMP
                     IVGGRFTSGFGMRFHPVLGYTRPHMGVDWAAPIGTPIFSAGNGTIVQAGWDSGYGRRI
                     EVQHANGYITTYNHLSGFARGATEGAHVRQGQVIGYLGQTGLATGPHLHYEILVNGHF
                     VDPMRVRLARTREFDGRMLANFKRERDRIDGLVLKAPNAPIVAERQP"
     gene            3008..3388
                     /locus_tag="VNY06_03215"
     CDS             3008..3388
                     /locus_tag="VNY06_03215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX13871.1"
                     /translation="MGLQSARLQPVAPACSLLAFAVLLCSINNTMAGAAPLNLVCNGN
                     SYKADGPFPTPEAYSLAIGGGKSVLIGGPGNAQPTKASIVANNPFQLKFRTSKFTAEY
                     FHFTGDLFLIHGDGHLTRLVCQPS"
     gene            complement(3721..4137)
                     /locus_tag="VNY06_03220"
     CDS             complement(3721..4137)
                     /locus_tag="VNY06_03220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012384630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MucR family transcriptional regulator"
                     /protein_id="HWX13872.1"
                     /translation="MSETSSSNSFIELAADIVSAYVSNNSVPVNDLPSLISDVHAALL
                     RVTSGAIGATTEAPKPAVPAKKSITREYLVCLEDGRKFKSLKRHLRTQYNMSPDEYRE
                     KWGLPPDYPMVAPDYAKARSNLAKQMGLGQQRRRKR"
     gene            4453..6600
                     /locus_tag="VNY06_03225"
     CDS             4453..6600
                     /locus_tag="VNY06_03225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020175458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HWX13873.1"
                     /translation="MPKLKRSLFSHSAAPPRIAKRRAEKIVHGVTLGDDYAWLKASNW
                     QEVLRDPVVLPDDIRTVIEAENDYAEATLAPAAELRATLVKEMRGRIKEDDCEVPNED
                     GAWFYFARHNAGGQHPVFCRVARSGGSEEVLLDGDAEGRGKSFFDIGTVRQAPDHTKL
                     GWSADEMGAELYEIRIRDLWTSEPGLSGSSPDRGEVIQDTSGEFVWMADSLGFYYVRM
                     DENHRPAEVYRHRIGTDSAADVCVFEAHDPGLFVHINRSQSARFAIISVSDHDASECY
                     LLDLFDHHAMARLIEPRSPGLRYDVEHHGDRLFIRTNAEGAEDFKIMSAPLSSPGRSS
                     WIEEVPHRRGRMIIRLAVYPNYLLRIEREAALPRIVVRHLASSEEHAIAFTDEAYSIG
                     LDERFEFNTDTFRFSYSSMTTPWETYDYNLATRERVLRKRQIIPSGHDPACYVTRRFF
                     ATAEDGEQVPISLLHRAGLRLDGSSPLLLYGYGAYGSHVPASFSTNRLSLVDRGFIYA
                     IAHVRGGTDKGWHWYTDGKFGKKTNTFNDFITVARHLIASGYTSAGRIIAQGGSAGGM
                     LMGAAVNLAPELFAGVIADVPFVDVLNTMLDENLPLTPPEWLEWGNPISDPAAFAVIR
                     SYSPYDNIEAKTYPPILALGGLTDPRVTYWEPLKWVAKLRDRVTGGGPILLNTNMDAG
                     HGGAPGRFTQLEEVALQYAFALGCVEGALETST"
     gene            complement(6611..>7262)
                     /locus_tag="VNY06_03230"
     CDS             complement(6611..>7262)
                     /locus_tag="VNY06_03230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX13874.1"
                     /translation="NTAAVDPHSELGSGPIVPASTAGEAEENTPQHPPPAEETYAETR
                     RNGGRDEPWPRQNNGGSEPLIVPPFPEMPIAADEVQSSPRNSWPTEPALIETILPEAG
                     HMPHEPVVEARREGTEKSLPEAQEEKNLQQPPLTSKAGIEASLGEWETGNEPPSDQAI
                     SGDTLAIVGRYEFDGTSYVRYADGSIEARTGHAVIHFKSMAELKTYMDSQAKKSEE"
BASE COUNT         1564 a         2007 c         2021 g         1670 t
ORIGIN      
        1 cgcctatgca gggctggtac cgacgccgtg gcggagcggc tcgatcgaca cgcgtgatac
       61 ttgcacattg atctgatcgg cgcagtggca aatgtttcac gtaaaacatt tctagaatag
      121 ttctaaatga ccatctttta gagccaagta tgatccttgc tccggccgta atctacggct
      181 gcctctcggc gacgataggc gcgttcggcg ccttgagtac caagccgtcg attcgatcac
      241 gttcgcgttt gaaattggca agcatgcgtc cgtcgaattc tcgcgtgcgc gcgagcctca
      301 cccgcattgg atcaacgaaa tgtccgttga cgaggatttc gtaatggagg tgggggccgg
      361 tggctaagcc agtttggccc aaatagccga tgacttggcc ttgccgcaca tgagcgcctt
      421 ccgtggcacc gcgggcaaac ccggacaggt gattgtaggt cgtgatgtaa ccgttggcgt
      481 gctgaacctc gatgcggcgg ccatagcccg aatcccatcc cgcttggacg atcgtgccat
      541 tgcctgcgga gaagatgggc gtgccgatag gggccgccca atccacgccc atatgcggac
      601 gcgtatagcc aaggactgga tgaaacctca ttccgaagcc ggaggtaaac cggcccccta
      661 caatcggcat acgaacgaga aatttacgtg tcgatcggcc atcttgatca tagtaatcaa
      721 tggaggcgtc atctggagtt tggaaacggt aatagcggta ggtttcattg cgcgcgttaa
      781 ttgccgcata aagcagctga ttgtgcgcct ctccctcttc gctgtcgtca tagagaacct
      841 cgaatgagtc gccagcgctc acgcctcttt gaaaatcgac gtcattggcg aaaatccgga
      901 caagttcatc gacgattgga cgtggaattt cttgtttcag cgcagtttcg taaaatgaat
      961 cataaagacg catcgcatct gagtcgtcgg cttcttcgga ttcgtcccgg cttggccgct
     1021 tggcgacgtg gcgcggcggg gtaacccgga cgtagccgcc gcgatcgttg accgcgatgt
     1081 tcgtttccaa agtctcgccg gaatagaccg aaagacgggc caaagtcata ttgctgcctg
     1141 agccatcgat atcggcgaaa aggagcttaa gccgcgctcc ttctgccacc gcggcgtcgc
     1201 cccgcgtgac tccaaacgcg ctgatcacat cgttgacttg ctccttactg agcccggcgg
     1261 cacgcagtac gttctcgatt gtctcgccgc ggcgcaccac aactagccgc tcctccattt
     1321 gagttacctg cgcggcttgc ggcgagcgcc cgacgctggt cacgttttcg ggaaccattc
     1381 gcacttcgat tgtggaaaaa ggggccgtcg tgatctgttc ggagggattg gcataggcga
     1441 gaacgcccgg aagattggcg cgggaggtgc gcatcaaaag aagttgcggc ggcaaggcaa
     1501 gcggtttagc gcccgcggcc atcgtgtttt tcacctgttc ggcgacctga gcctgaacct
     1561 cctcgatcga caatgctgcc gacgccgaaa aggattgatc tcctaaatca cgggtggtcc
     1621 acgcgatttc ggcgtcgtcc tgaaccagct cctgcgcggc atcaattggc gcgcgcgcat
     1681 cagcaagcac ttttagcgga ttgtaggtcg ggacgtcttc ggagaggccg aggctcgcca
     1741 atgtcaggga cgtttggatg tgggtaaaag tatgaagctt cacgacttcc ttgtcgccgg
     1801 cccggatcgc tgcttgcgcc tggaagcgct gctttccggc gccgatatcg accgccttga
     1861 cgaggcgatc gcccttacgc ggattgacgc cgaaatctaa attgatctcc ttgcgctgcg
     1921 aaagcgcggg gaacggcaac tcggtgaaat aagtttgatg ccccagtgcc gcgtagatcg
     1981 ctgcgccaat gaggcctgca cccgaaattc cagtgaggag agtcccggaa agccagcgca
     2041 aagagacccg tcgccggtcg aatgttgcgg cacgagggcc aacggcttca atggcgggat
     2101 ccagcccaaa ttcattatct ccgcgcagct gagcccgttg caggtaaaca ccgtaagttt
     2161 gaatttgccc gagcccaccg attgacattg cgttgcttat ttcctggcaa ctgcggccca
     2221 tttgtcatga gcctggtctc gagtgacaca acataacgaa acccgcaacc gagcgccttg
     2281 aaatcgtgaa tcaaaccgtt ttgccgcaac tgcagacata cgcgaatgga acaagcgacc
     2341 ttccctccac aaggccgaag ggcaaagcgt catcgcggtc gaagcaaagc ttttgaaaat
     2401 ggctcaaata cggcgtagct catggaagtt tcccgaggca tacgactaag gtattggtat
     2461 gttcaaacgc gctcttgtaa aaattaactc attagcgctc gagcgggtgg actttcgaaa
     2521 aagtctgcat cccctaaagt aagacacgct ctgctcttgt cgccaagtga ccaagcaggc
     2581 gcttaatccg taaaatttga agcgaagtct ctcatagttg gatttgagaa ggaacctcgg
     2641 ccggatgtca aggagctttg gacgccgact gcagcttgaa cttctaaggt ctaagcagct
     2701 attcgggcta catcgaacga ttcaagagct cgttgcagtg cgatgagcgc cggagtccct
     2761 aaattattgt gcgcctccgc tcagttccat ctcattgatc gcaagcgaga actgcgcatt
     2821 gtatccacat ttggatgggt atagggtgcg caattatcgc agccgaatgc cttatgtcgt
     2881 ggaacaaatg cttaggtcat ggtctttggt tcttcgtcag ccgtcgcaaa ttttggattg
     2941 cccacgaaat ctgaacgttt ttgggcagca ccggcgcaga ccatcattct agtttgagga
     3001 ggatcaaatg ggacttcaat cagcaagact tcaaccggtt gcgccggcgt gctcactgct
     3061 cgcattcgcg gtgctgcttt gctcaataaa caatacgatg gccggagctg cgccactcaa
     3121 ccttgtctgc aatggaaata gttacaaagc ggacgggccc tttcccacgc cggaggcata
     3181 ttcgcttgca ataggcggag ggaaatcggt cttgattggt gggccaggca atgcgcaacc
     3241 gaccaaggct tcgattgtcg ccaataatcc ctttcagttg aaattcagaa cgagcaaatt
     3301 tacagccgag tactttcatt tcaccggcga tttgttcctg atccacggcg acgggcacct
     3361 caccaggctc gtttgtcagc catcctgaac acatcaatgc gtttgcctga gctggaaagg
     3421 gacgcaaggc agcgcagtcg cgcgcctaca atggagcatg acgcttgggc cgcgccacgc
     3481 gtatcgagac tccgaatcga tacaagttgc ggccgacacg ttggtaggtg ccataatgaa
     3541 atggccgcgc agacgggaac caacatatca ggattcccat ccgtgattgt taacttcaat
     3601 actatgcgtc aaaacctaaa aatattatgc gccagaacct caacaaaaat tacgcggtaa
     3661 cagcgcgctg cgccgtaatt tcatccaatg ccggagcgtt ttgaaagctt accgccgtgg
     3721 ttaccgtttg cggcggcgtt gctggccaag tcccatctgc tttgcaagat ttgagcgcgc
     3781 tttggcgtaa tccggagcga ccatcggata atccggcgga aggccccatt tctcgcgata
     3841 ttcgtccgga gacatattat attgcgtccg cagatgcctt ttcagggatt taaacttccg
     3901 gccatcctcc agacaaacga gatattctct cgtgatagat ttcttcgccg gcactgccgg
     3961 ttttggcgcc tcggtggtgg cacctattgc gcccgatgtc acgcgcagca atgccgcatg
     4021 cacgtcgctg atcaaggatg gtagatcatt tacaggcacc gaattgttac tgacataggc
     4081 tgaaacgatg tctgcagcta gctcaatgaa actgttagaa cttgaggttt cgctcatagc
     4141 gccctgcatc tcagagtaga acgtgtcgcc ggtagtatac gggtcaaata gcgatcgcct
     4201 attttatgcc cgttgtcaca agaccaacca gaagaaagct cttttaagcg atttcccgcg
     4261 aatcaacttt caagaacttc tagcccttct aaggggaagg cttgttttgt gcaagtagca
     4321 atacgaaaac ttgagtgcaa ttaagcaaaa aggagttgaa agacagggga ccagcgtgaa
     4381 taacgccgct gcgacgcgct aaacatccct tcttacctat gcggcgcgag ggggcagctt
     4441 cagggaatat agatgcccaa gcttaaacgt tccctttttt ctcactcggc cgctcctccc
     4501 cgcatcgcaa agcgccgggc ggaaaagatt gtccatgggg ttactctcgg cgacgactat
     4561 gcttggctga aggcatccaa ctggcaagaa gtcttgcgcg atccggtagt tttacctgac
     4621 gacatccgca ccgtgatcga ggccgagaat gattatgcgg aggcgacact cgcacctgca
     4681 gccgagttgc gcgccacgct cgtcaaagag atgcgcggca ggattaagga agatgattgc
     4741 gaagttccga atgaagatgg ggcctggttt tactttgcgc gccataatgc ggggggacaa
     4801 catccggtgt tctgcagggt tgcgcgcagc ggcggctccg aagaggtcct gctcgatggc
     4861 gacgcggaag ggcgaggcaa gagctttttt gacataggca cggtccgaca ggcgccagat
     4921 cacaccaagc tgggctggag cgccgatgag atgggcgctg agctctacga gattcggatt
     4981 cgcgatctct ggacctccga gcccggcctc tcgggcagtt cgcctgatcg cggagaagtg
     5041 atccaagata ccagcggcga gtttgtgtgg atggccgatt cgctcggctt ttactatgtg
     5101 cgaatggacg aaaatcaccg cccggctgaa gtctaccgtc accgtatcgg aacggattct
     5161 gccgccgatg tttgcgtctt tgaagcccat gaccctggcc tcttcgtcca catcaaccgc
     5221 agccaatcgg cgcgctttgc aatcataagc gtcagcgatc acgatgcttc cgaatgctat
     5281 ctgctcgatc ttttcgatca ccacgcgatg gcgcgactta tcgagcctcg ttctccggga
     5341 ctgcgttatg atgtcgaaca tcatggcgac cggcttttta tacgaaccaa tgccgaggga
     5401 gcggaagatt tcaagatcat gagcgcgccc ctttcgagcc cagggagatc gtcctggatc
     5461 gaagaggtcc cgcatcggcg agggcgcatg atcattcggc ttgctgttta tcctaattat
     5521 ctcttgcgta tcgagcgcga ggcagcgctg ccgcggattg tcgtgcgcca tctcgcaagc
     5581 agcgaggagc atgcaatcgc atttaccgac gaggcttatt cgatcggtct tgatgagcga
     5641 ttcgaattta acacggacac ttttagattt tcctattcgt caatgacgac gccgtgggaa
     5701 acctatgact acaatctcgc gacccgcgaa cgtgtcctgc gcaagcgtca gatcattccg
     5761 tcaggtcacg atcctgcgtg ctatgtgacg cggcggttct ttgccactgc ggaggatggt
     5821 gagcaagttc cgatctcctt gcttcaccgg gctggactgc gcctcgacgg gtcctctccc
     5881 ttgttgcttt acggctatgg cgcctatggc tctcacgtgc ccgcctcctt ttccaccaat
     5941 cggctgagcc tcgtcgaccg gggattcatc tatgcaatcg cccatgttcg aggcggcacg
     6001 gacaagggct ggcattggta cacggatggg aagttcggca aaaaaacgaa tactttcaat
     6061 gacttcatca ccgtagcgag acatctcatc gcgtctggct atacgagcgc gggccgaatc
     6121 attgcgcagg gcggcagcgc ggggggaatg ctcatgggtg ccgccgtcaa tctcgcgccg
     6181 gaattgttcg cgggtgtcat cgccgacgtc cctttcgtcg atgtcctgaa cacgatgctc
     6241 gacgaaaacc tgcctttgac gccgccagag tggctcgaat gggggaaccc aatttctgat
     6301 cccgccgcat tcgcggtcat ccgctcttat tctccttacg acaatatcga ggcaaaaact
     6361 tacccgccaa ttctcgcact gggaggtctc acagatccgc gtgtgaccta ttgggagccg
     6421 ctcaaatggg ttgctaagct gcgcgacaga gtgacgggcg gtggtccaat tctgctcaac
     6481 accaatatgg acgcggggca tggcggcgcg ccaggacgct ttactcagtt agaagaagtc
     6541 gctctgcaat atgccttcgc gcttggctgc gtcgaaggcg cgctcgagac cagcacctga
     6601 tcttccgtgc ctactcttcc gatttttttg cttgagaatc catgtaagtt ttcagttcgg
     6661 ccatggattt gaagtgaatg acggcgtggc ctgtccgcgc ttcgatcgat ccgtccgcgt
     6721 acctgacgta tgaggtaccg tcgaattcgt aacgtccaac gatggcaagt gtatcgccgc
     6781 tgattgcttg atccgaaggc ggctcattgc ccgtttccca ctcccccaga ctagcctcaa
     6841 ttccagcttt tgaagtaaga ggcggttgct gtaaattttt ctcttcctgc gcttcgggca
     6901 aagatttttc tgtaccttcc cttctcgctt ccacgacggg ttcgtggggc atgtgccctg
     6961 cctccggcaa gatcgtctct attaaagcag gctcggtagg ccagctattg cgcggcgagg
     7021 actggacttc gtcggcagca attggcattt cggggaatgg cggcacaata agcggctcgc
     7081 ttccaccatt gttttggcgc ggccatggct cgtcgcgccc cccgtttcgc ctggtctcgg
     7141 cataggtctc ttcggccggc ggcgggtgtt gcggcgtgtt ctcttcggct tcgcccgcgg
     7201 ttgaggcggg tactatcgga cctgagccca gctccgaatg cggatctacc gccgctgtgt
     7261 tt
//
LOCUS       DATMPU010000140         2010 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_3212179,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000140 DATMPU010000000
VERSION     DATMPU010000140.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 2010)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2010)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2010
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3212179"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1654
                     /locus_tag="VNY06_03235"
     CDS             <1..1654
                     /locus_tag="VNY06_03235"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HWX13875.1"
                     /translation="ARIEYRIGINLGDIIVEGDDIFGDGVNVAARLEALAEPGCLCIS
                     ASAYEQVRDKVGFKFRDSGEQTVKNIARPIRAFTTRPGTGAAARQPRSAPSTGRRTLL
                     TIGAAAATIILAAGAIYWWMSAQPKRPLPTGAPAVGQAEASRPAIALPDKPSIAVLAF
                     NNMSDDTKQDYFSDGISEDLITDLSKISGLFVIARNSSFVYKGKAVSIALVAQELGVR
                     YVLEGSVRKAANRIRINAQLIDATTGTHCWAERYDRQLEDVFAVQDELARTIAAVLAA
                     QVNRVEAEHTLLRPPATWQAYDFYMRAADLLVSFQSTYKLEELYEARRLLERCLALDA
                     NYARAHAAYSFAWIVAWINSLDGDFLSPVALDRAHLFGRNAVQLDRNLPQGHAQLGYV
                     LAFEAQHELSIAEFERAIELNPNFTDRRFAIPLIFAGQFDRAVQCIQMHKRADPFYFP
                     VASGFLGLALYMLKRYSEALTPLLECVSRAPNLRDGHVWLAATYAQLERPEQARQQAA
                     EVLRIEPTYTTERTAKPIMRFRNASDADHLFDGLHKAGLPRD"
BASE COUNT          406 a          623 c          585 g          396 t
ORIGIN      
        1 cgcgcgcatc gagtatcgca tcggcatcaa tctcggcgac atcattgtcg aaggcgacga
       61 tatctttggc gacggcgtca atgtggcggc ccggctcgaa gcgctggccg agcccggttg
      121 cctctgcatc tccgcctcgg cttacgaaca ggtgcgcgac aaggtcggct tcaaatttag
      181 ggatagcggc gagcagacgg tgaagaacat cgctcggccg atccgcgcct tcaccacgcg
      241 gccggggaca ggtgccgccg ctcggcagcc gcgaagcgct ccgtcgactg gccgccggac
      301 gctgctgacg attggcgcag ccgcggccac gatcattctg gccgccggcg cgatctattg
      361 gtggatgtcc gcgcagccga aacgcccgct gccgactggc gcgcccgccg tcggtcaagc
      421 cgaagcgagc cgacctgcga tagcgctgcc cgacaagccg tcgatcgccg ttctcgcttt
      481 caacaacatg agcgacgata ccaagcagga ctacttcagc gacggcatct cggaagatct
      541 catcaccgac ctgtcgaaaa tctccggctt gttcgtaatc gcgcgaaatt cgagcttcgt
      601 ctacaagggc aaggcggtat cgattgctct ggtcgcccaa gagctcggcg tccgctacgt
      661 gctcgagggc agcgtccgga aggccgccaa ccgcatccgg atcaacgccc agctcatcga
      721 cgcgacgacg ggcacgcatt gctgggccga gcgctacgac cgtcagctcg aggatgtctt
      781 cgcggtccag gatgagctcg cgcggacaat tgcggcggtc ctcgccgcgc aagtaaacag
      841 ggtcgaggcc gagcacaccc tgctcagacc gccagcaact tggcaagctt acgacttcta
      901 catgcgcgcc gccgatcttc tcgtctcgtt ccaatcaacc tacaagcttg aggagctcta
      961 cgaggcgcgg cgcctacttg agcgctgcct tgcactcgat gcgaattatg ctcgcgcgca
     1021 tgctgcctac tcctttgcct ggattgtggc ttggatcaat tcgctggatg gtgatttctt
     1081 gagtccggtt gcacttgatc gagcccatct attcgggcgt aacgctgtgc aactcgatcg
     1141 aaacttgccc caaggccacg cgcaactggg atacgtgttg gcattcgagg cgcagcatga
     1201 attgtcgatt gccgagttcg agcgcgcaat cgaactgaac cctaacttta ccgatcgccg
     1261 cttcgctatt cccctcatct ttgccggtca gtttgataga gccgttcagt gtattcagat
     1321 gcacaagcgt gccgacccct tctactttcc cgtcgcctcc ggcttccttg gcttggccct
     1381 atacatgctg aagcgttatt ccgaggcgct cacacctctg ttggagtgtg tttcgcgcgc
     1441 cccgaatttg cgagacggtc acgtgtggct agcggcgacc tacgcgcaat tggagcggcc
     1501 tgagcaagcc cgtcaacaag cggccgaggt actccggatt gagccaacgt acacaacgga
     1561 gcggacggca aaaccgatca tgagattcag gaacgcgtcg gacgcagatc atctctttga
     1621 cggcctgcat aaagcaggcc tgccgcggga ctagcaacgc ggcggtaaga aaccaagcag
     1681 aggtctgcaa tggggcacac tgagaagaac tcggtgcgag catatgccgt ccgggttgcc
     1741 cctgagagcg gacctttctc aatgcagtcg gcatgtctca caggggccaa taggtgacat
     1801 cgcaacgggt tcaagcgagc tgtcggcgtt ctcctggctg acatgtcgac ggcgcgacac
     1861 caggcaaata tattgcgaga aagcgtcttg atgcgtggcc gctattggag ctgcggagat
     1921 gagcaaaacc agaagttttc catggatctc cagccttcct taactgccgc cggcgaccaa
     1981 aaccaacgaa gccttcaaag ctttcatcgt
//
LOCUS       DATMPU010000141         2362 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_1978092,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000141 DATMPU010000000
VERSION     DATMPU010000141.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 2362)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2362)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2362
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1978092"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..526
                     /locus_tag="VNY06_03240"
     CDS             <1..526
                     /locus_tag="VNY06_03240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX13876.1"
                     /translation="FRFRIVAGIEIDNSAAGISGQTTVDGSFPSGTPYSTTFGSRGDD
                     IGTARLRLGYSWGRFLPYLTAGFTFGVIETPYDFSTPGFFSSAESTAVRSGVFPHVGN
                     GGMGVENAINSNLTVKTVYLYEFINARRTSFSPGDGSTIAFGTRTMYHIVRLGLNCRF
                     DWLSPVPAPVAARY"
     gene            complement(644..>2362)
                     /locus_tag="VNY06_03245"
     CDS             complement(644..>2362)
                     /locus_tag="VNY06_03245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020174212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HWX13877.1"
                     /translation="QRVARLMPTADALQMLFKGDQIKPKAAKTMNLVHEVTPAAEIVA
                     KAKAWIAAGGKARAPWDEPNFRLPSGKVFSPQGMMIWPPANAIYRRETHNNYPAAKAI
                     LHSVYEGLQLPFDLALRVEARWFARILRSPEAAAMIRTLFISMGELNKGVHRPAAIPA
                     STIRRVGIIGAGFMGAAIAYVTASAAMEVVLIDRDQQQADKGKEICDKLISGQVLRGR
                     AKAADKEALLTRIKPTAEYNNLAGSDLVIEAVFEDRAVKADVTQRAHAVLSPGTIFAS
                     NTSTLPITSLAQAYGEPENFIGLHFFSPVEKMQLVEVIVGEKTGDKALAMALDFVRAI
                     KKTPIVVQDSRGFYANRCVLNYVHEGHIMLLEGVPPAMIENVARMAGMPVGPLALNDE
                     VGLDLALNIIEVAKRDLGKDGVDPAQEILLRTMVAGHGRSGRKNGKGFYDYPAQGPKS
                     LWPGLETFQPSKLDPDRIDIRELKQRFLVIQAVEAARVMQEGVVTDPREADVGSIIGF
                     GFAPFTGGTLSYIDGMGAANFVALCEKLRQQYGKRFTPPQILIDMAKSHETFYGRFAG
                     QSKRVA"
BASE COUNT          486 a          707 c          640 g          529 t
ORIGIN      
        1 cttccgcttc cgcattgtgg ccggaatcga aatcgacaac agcgcggcag gcatcagcgg
       61 ccagacaacc gtcgacggct catttccctc ggggacgccg tattccacca ccttcggctc
      121 caggggcgat gatatcggca ccgcgcggct acgtcttggc tactcttggg gccgcttctt
      181 accctatctc acagccggtt tcacctttgg ggtgatcgaa accccctacg atttctcaac
      241 gcctggattc ttcagctcgg cagaaagcac ggcggtccgc tcgggcgtat ttcctcatgt
      301 gggaaacggt ggcatgggcg tcgaaaatgc catcaactcc aatttaacgg taaaaaccgt
      361 atatctctac gaattcataa atgcccggcg aacatcgttt tctcccggag acggaagcac
      421 catcgccttc ggcacgcgca caatgtatca tatcgttcgg ctgggattga actgtaggtt
      481 cgattggctg tcgccggttc cggcgcctgt tgccgcaaga tactaaagcg ttttcttgtc
      541 gcatcaaacc tcttcctgcc gactcgaagc ttcttgaccg agaggctagc tgtcgctctg
      601 aatcgatttt tgagggaaaa agcgcgcccg atttcgggac aaattacgcc acgcgcttcg
      661 actgcccggc gaagcgccca taaaatgttt cgtggctttt cgccatgtca atgaggattt
      721 ggggcggggt gaagcgcttg ccgtattgtt gccggagctt ttcgcaaagc gcgacgaagt
      781 ttgccgcgcc catcccgtca atatagctca gcgttccccc ggtaaacggc gcgaaaccaa
      841 agccgatgat cgaccccaca tcggcctcgc gcggatcggt aacgacaccc tcttgcatca
      901 cgcgcgcggc ctcaaccgcc tggatcacga gaaagcgttg cttcagttcc ctaatatcga
      961 tccgatccgg atcgagcttg gagggctgga acgtttcaag tcccggccag agactctttg
     1021 gaccctgcgc cggataatca taaaagcctt tgccattctt gcgcccagac cggccgtgac
     1081 cggcgaccat agttctaagc aaaatctcct gcgccggatc gaccccgtct ttcccaagat
     1141 cacgctttgc cacctcaatg atgttcaatg cgagatcgag cccgacctcg tcgttcagtg
     1201 cgagcggccc aaccggcatc cccgccattc tggcaacatt ttcgatcatt gcgggcggaa
     1261 cgccctcaag cagcatgata tggccttcgt ggacataatt gaggacgcag cgattggcgt
     1321 aaaagccgcg cgagtcttgc acgacaatcg gcgttttctt gatcgcgcgc acgaaatcca
     1381 aagccatggc gagcgctttg tcgccggttt tctcaccaac aatcacttcg acgagctgca
     1441 tcttctcgac tggcgagaaa aaatgaaggc caataaaatt ctccggttcg ccataggctt
     1501 gcgcaagcga ggtgatcggc agcgtcgagg tattcgaagc gaagatcgtg ccgggggaga
     1561 gaacagcatg tgccctttgg gtgacatccg ctttgaccgc gcgatcttcg aacactgctt
     1621 cgatgacgag gtccgagcct gcaagattat tatactctgc cgttggctta attctcgtaa
     1681 gcagcgcttc cttatcggcg gccttggcgc ggcctcttaa cacctgcccg gaaatgagct
     1741 tgtcgcagat ctccttgccc ttgtctgcct gttgctggtc cctatcgatc agcacgacct
     1801 ccattgccgc gcttgctgtg acataggcga tggccgcgcc cataaagcca gcgccgatga
     1861 tgccgacgcg gcggattgtg gacgcaggaa tggctgcggg gcggtgcacg cccttgttca
     1921 attcgcccat cgagatgaat agcgtgcgaa tcatcgccgc cgcctcaggc gaacgcaaaa
     1981 tcctagcaaa ccagcgtgct tcgacacgca gcgccaaatc gaatggcaat tgcaagcctt
     2041 catagacgct gtgcaaaatc gccttcgccg ccggataatt gttgtgggtc tcgcgccggt
     2101 agatcgcatt ggctggtggc caaatcatca tgccttgggg cgagaatact tttccggaag
     2161 gaagcctaaa gttcggttcg tcccaagggg cacgtgcctt tccgcccgcc gcaatccagg
     2221 ccttggcctt ggcgacgatt tcggccgcgg gcgtgacctc atgtacgagg ttcattgttt
     2281 ttgccgcttt cggcttgatc tgatcaccct taaaaagcat ctggagcgca tcggcggtcg
     2341 gcatcaggcg ggcgacgcgc tg
//
LOCUS       DATMPU010000142         2609 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_2591843,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000142 DATMPU010000000
VERSION     DATMPU010000142.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 2609)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2609)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2609
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2591843"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..182)
                     /gene="ndhC"
                     /locus_tag="VNY06_03250"
     CDS             complement(<1..182)
                     /gene="ndhC"
                     /locus_tag="VNY06_03250"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003608837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HWX13878.1"
                     /translation="MSSLFDQYLPLVIFMAVAALISGALLLAPFFAAYKAPDPEKLSV
                     YECGFNAFDDARMKFDV"
     gene            complement(397..825)
                     /locus_tag="VNY06_03255"
     CDS             complement(397..825)
                     /locus_tag="VNY06_03255"
                     /inference="COORDINATES: protein motif:HMM:NF036806.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX13879.1"
                     /translation="MAEEKLGAKEAGHGFFSSDAEASLDGPFWDELDLPGSTGARSER
                     ADFETTPGGGRTSLPGSGDPCPPVDLHRETPQADIATRVTELLGPAPVSVDELARLAD
                     APARQVRTVLMELELAGRIEWLGGDLVSSLPVSRPLSNDN"
     gene            complement(865..1299)
                     /locus_tag="VNY06_03260"
                     /pseudo
     CDS             complement(865..1299)
                     /locus_tag="VNY06_03260"
                     /inference="COORDINATES: protein motif:HMM:NF014533.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
     gene            complement(1354..1677)
                     /locus_tag="VNY06_03265"
     CDS             complement(1354..1677)
                     /locus_tag="VNY06_03265"
                     /inference="COORDINATES: protein motif:HMM:NF045295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX13880.1"
                     /translation="MAQDPLSKPLTALDEGEKFDWLELLRCEYIGPRTFQRLLNRHGS
                     AGAALAALPELIASGKAGRPIRIATIEETEREIEATLNLADALSPCASRSIRCFCAAR
                     TRHRR"
     gene            complement(1677..2261)
                     /gene="plsY"
                     /locus_tag="VNY06_03270"
     CDS             complement(1677..2261)
                     /gene="plsY"
                     /locus_tag="VNY06_03270"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012592806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HWX13881.1"
                     /translation="MLQNVSAFFFGYFCGSIPFGLILTRAVGTVDLRTIGSGNIGATN
                     VLRTGRKGLAALTLLLDLLKGTAAVLIAAHFARGNPMLLAALGAFLGHLFPVWLNFKG
                     GKGVATFLGCLLATAWPAALGFGVIWLAVAYFSRYSSAAALAASALTPIVLAVLGENG
                     AALTFGFMAILLWERHWANIVRLAAGTESRIGSS"
BASE COUNT          588 a          810 c          767 g          444 t
ORIGIN      
        1 acgtcgaatt tcattcttgc gtcgtcaaaa gcgttgaagc cgcattcgta aacagagagt
       61 ttctctggat cgggtgcttt ataagcggcg aagaagggcg caagaagcag ggcgcctgat
      121 atcaaggcgg caactgccat aaagatcacg agcggcagat attgatcaaa taagctcgac
      181 atggcgcgcc tcggacaaaa cctgcggggc caggagcgaa gtcgattgcg gccggcctgc
      241 gcccccaggc gatccgtacc gcagtgcaat gaaatgcgca agatggcagc gagaaatttg
      301 cgccagcgca gctttctcct agcctctgga agcggcgatg agaaatgtat agcagcctta
      361 gcgtgctaag gcggccgcag caatcattcg ctgttatcaa ttgtcattag agagcggccg
      421 agacaccggc agggaggaaa cgagatcacc cccgagccat tcgatcctgc cggcaagttc
      481 gagttccatt aaaacggttc gcacctgccg tgccggagca tccgccaagc gagcaagttc
      541 atcgaccgag accggcgctg gccctaagag ctcggtgacg cgcgtcgcga tatcggcctg
      601 cggggtctcc ctgtgcaagt caaccggtgg acaggggtcg cctgaaccgg gcaagctggt
      661 tctgccgcca cccggcgttg tttcgaagtc tgcgcgctca gagcgcgccc ccgttgagcc
      721 gggaaggtca agttcatccc aaaaaggccc gtcgaggctc gcctcggcat cggaactgaa
      781 aaaaccgtgg ccggcctctt tcgccccaag cttctcctct gccattgcat caagcacgtc
      841 ttccggcttg gtgcagaacg acgccccgtc gcgcagcaac tggttggcgc cttccgcgcg
      901 cggatcgagc ggcgagcctg gaactgcgaa aacttcccgc ccctgttcgg cggcgaatcg
      961 tgcagtgatc agggagccgg accgccgcgc cgcctcaacg acgacgaccg cccggctcag
     1021 ccccgcaaca atgcggttcc ggcgcggaaa atcgcgtccc cgcgcctccc agccgaaagg
     1081 catttcgctg atcgcggctc cttgctcgag tatttgctcg agcaagtttt ggtgctcagc
     1141 cgggtaaata ttgcccaaac cacctgcaac gaccgcaatc gttccggtct cgattgcggt
     1201 ttgatgtgcc cgtgcgtcaa tgccacgcgc aagacccgag acaatcacat ggccggcccg
     1261 gccaatggcg cgagcgagct gggccgcgag ggcaagcccc gaacaccgcg atcttcgatc
     1321 gctgaaggct caaaagattg ccgcgaacgg caatcagcgg cggtgccgag tccgtgcggc
     1381 gcaaaagcat cggatactcc ggctcgcaca gggcgataaa gcgtccgcca agttaagggt
     1441 tgcctcaatc tcgcgctcgg tctcttcgat cgtcgcgatg cggatcggac gtccggcttt
     1501 gccgctcgcg attaattccg gcaacgcggc aagcgccgcc ccagcgctcc cgtggcggtt
     1561 gagcaggcgc tgaaacgtcc gcgggccgat atattcgcag cgcagaagct ccagccaatc
     1621 aaacttctcc ccctcatcta aagcggtaag cggcttgctg agcggatcct gcgccatcag
     1681 cttgacccga tccgcgactc cgttcctgcc gcaagccgaa cgatgttggc ccaatgcctt
     1741 tcccacaaaa gaatcgccat aaagccaaat gtaagcgctg cgccgttttc gccgaggacg
     1801 gcgaggacaa ttggggtcag cgcgctcgcg gcgagcgctg cggccgagga gtaacgggag
     1861 aaataagcaa ccgcgagcca gattacgcca aagccgagcg ctgccggcca ggcggtcgca
     1921 agaaggcagc cgaggaaggt cgcaacgccc tttcctccct taaaattcag ccaaaccgga
     1981 aacaaatggc cgagaaaagc gccaagcgcg gcaagaagca taggattgcc acgcgcaaaa
     2041 tgagcggcga tcagaaccgc ggcggttccc ttgagcaagt caaggagcag ggtcagcgcg
     2101 gccaagccct tgcggcccgt gcgcaaaaca tttgtcgcac caatattgcc ggaaccgatt
     2161 gtgcgaaggt cgacggtacc gaccgcgcgc gtcaagataa gtccaaacgg aatggagccg
     2221 caaaaatagc cgaaaaaaaa agccgaaaca ttttggagca ctttgggaaa ccggcaagtt
     2281 ttaaggcggg tgatagatta ccctaacctt ccaaaggcga agcggcaacc cgctggcgga
     2341 gggcctctga tccacatctc tttgcatcga gacccgcatg gcaaagcgaa gtgctcattt
     2401 acggatgctt agcccaaccg ttgccgctca gctagcgcgc ttgaccaagc caaagttagg
     2461 gcctacccag ggaatgggac acctttcccc aacgaaaggc gcccatctga aaggatggct
     2521 attatggcat tgacactccc cgccgcgcgg cccgcccggc cgcactttgc ttgtggtcct
     2581 tgcgccaagc atccgggctg gcagcttca
//
LOCUS       DATMPU010000143         8435 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_4448659,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000143 DATMPU010000000
VERSION     DATMPU010000143.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 8435)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8435)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8435
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4448659"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..318
                     /gene="rplD"
                     /locus_tag="VNY06_03275"
     CDS             <1..318
                     /gene="rplD"
                     /locus_tag="VNY06_03275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016843745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HWX13882.1"
                     /translation="KKLRVLALKHALSAKAKEGQIMVFAEVRSTDAKTKSLRTSLRKL
                     GLTNALIIDGTEVATNFQLAARNIPQIDILPVQGINVYDIIRREKLVLTKAAIEVLEA
                     RFK"
     gene            315..626
                     /locus_tag="VNY06_03280"
     CDS             315..626
                     /locus_tag="VNY06_03280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012384353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HWX13883.1"
                     /translation="MSPSKLGHDSQHYEIILAPVITEKATMASEANQVIFKVRKDATK
                     PQIKAAVERLFDVKVAAVNTLIRKGKRKLFKGTRGVQSDVKKAVVTLAEGHKIDVTTG
                     L"
     gene            648..1487
                     /gene="rplB"
                     /locus_tag="VNY06_03285"
     CDS             648..1487
                     /gene="rplB"
                     /locus_tag="VNY06_03285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012589619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HWX13884.1"
                     /translation="MALKTFKPVTPSLRQVVIVDRTGLYKGKPLKQLTEGKSSSGGRN
                     NNGRVTVRFRGGGHKQSYRIVDFKRRKLGVAAKVERIEYDPNRTAFIALIRYADGEHA
                     YILAPQRLAAGDEVIAGLQADVKPGNAMALANIPVGTIVHNIEMKIGKGAAIARAAGS
                     YAQVVGRDEGYVIMRLTSGEQRLIHGQCFATIGAVSNPDHMNISHGKAGRSRWLGQRP
                     HNRGVTMNPVDHPHGGGEGRTSGGRHPVTPWGKPTKGKKTRSNKSTTKFIVTSRHKSK
                     KKG"
     gene            1490..1768
                     /gene="rpsS"
                     /locus_tag="VNY06_03290"
     CDS             1490..1768
                     /gene="rpsS"
                     /locus_tag="VNY06_03290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012384355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HWX13885.1"
                     /translation="MARSIWKGPFVDGYLLKKAETARNSGRSEIIKMWSRRSTILPQF
                     VGLTFGVYNGHKHIPVSVTEDMIGHKFGEFSPTRTFHGHAGDKKAKRG"
     gene            1781..2170
                     /gene="rplV"
                     /locus_tag="VNY06_03295"
     CDS             1781..2170
                     /gene="rplV"
                     /locus_tag="VNY06_03295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012384356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HWX13886.1"
                     /translation="MGKEQAPRALKENEAKAVARMLRVSPQKMNLLAQLIRGKKVDKA
                     LADLAFARKRSALDVKKTLESAIANAENNHNLDVDDLVVAQAYVGNAMILKRFSARAR
                     GRSGRLRKPFSHLTIIVRETGADASQA"
     gene            2181..2894
                     /gene="rpsC"
                     /locus_tag="VNY06_03300"
     CDS             2181..2894
                     /gene="rpsC"
                     /locus_tag="VNY06_03300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008969405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HWX13887.1"
                     /translation="MGQKVNPIGLRLGVNRTWDSRWFAGKAEYGKLLHEDMAIRATLM
                     KNLKQAAISKIIIERPHKKCRVTIHSARPGVVIGKKGADIDKIRKTITKMTDSEVVIN
                     IVEVRKPEIDAVLVADSIAQQLERRVAFRRAMKRAVQSAIRLGAEGVRINCSGRLGGA
                     EIARLEWYREGRVPLHTLRADVDYGTATAHTAYGTCGIKVWIFKGEILEHDPMAQDKK
                     LTEQDRTGGERRRRDRDAA"
     gene            2927..3340
                     /gene="rplP"
                     /locus_tag="VNY06_03305"
     CDS             2927..3340
                     /gene="rplP"
                     /locus_tag="VNY06_03305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005973000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HWX13888.1"
                     /translation="MLQPKRTKFRKAFKGRISGAAKAGFELNYGQFGLKALEPERITA
                     RQIEAARRALTRHMRRAGRVWIRIFPDVPVSKKPTEVRMGKGKGAPEYWVCRVAPGRI
                     MFELDSVSADLAQEALRLAAAKLPIKTRFVQRIVE"
     gene            3350..3574
                     /gene="rpmC"
                     /locus_tag="VNY06_03310"
     CDS             3350..3574
                     /gene="rpmC"
                     /locus_tag="VNY06_03310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015928594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HWX13889.1"
                     /translation="MKQRQRLSDLKVMSNDQLEQELLNLKKEQFNLRFQRATGQLENT
                     ARVRTVRRDIARIKSLSAQKRREGAKLKQE"
     gene            3599..3838
                     /gene="rpsQ"
                     /locus_tag="VNY06_03315"
     CDS             3599..3838
                     /gene="rpsQ"
                     /locus_tag="VNY06_03315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012384360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HWX13890.1"
                     /translation="MPRRILQGVVVSDKQDKTIVVKVERRFTHPLLKKTVRRSKKYHA
                     HDETNAHKAGDAVSIEETKPISKLKRWSVVKVAGK"
     gene            3925..4293
                     /gene="rplN"
                     /locus_tag="VNY06_03320"
     CDS             3925..4293
                     /gene="rplN"
                     /locus_tag="VNY06_03320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011567679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HWX13891.1"
                     /translation="MIQMQTNLDVADNSGARRVMCIKVLGGSKRKYAGVGDTIVVSIK
                     EAIPRGRVKKGDVMKAVVVRTAKDIKRPDGSVIRFDSNAAVLLNNQSEPVGTRIFGPV
                     PRELRARNHMKIISLAPEVL"
     gene            4293..4610
                     /gene="rplX"
                     /locus_tag="VNY06_03325"
     CDS             4293..4610
                     /gene="rplX"
                     /locus_tag="VNY06_03325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012384362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HWX13892.1"
                     /translation="MAAKIKKGDRVVVLAGRDKGRAGEVVGVAPSEERAIVRGLNMVQ
                     RHHRQTAKQEGGIVSKEAPIHLSNLAIADPKNGKPTRVGFKTLPDGRKVRFAKRSGEL
                     IDG"
     gene            4603..5355
                     /gene="rplE"
                     /locus_tag="VNY06_03330"
     CDS             4603..5355
                     /gene="rplE"
                     /locus_tag="VNY06_03330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012589610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HWX13893.1"
                     /translation="MAEAKTGKDGSKGPGKSAAKVSAKGSPKDSPKGAAKEAAKSGKS
                     AKAPAGHGREDGARAAPAKDFAPPTDYAPRLKKYYADVVQPRLIEQFGYKNRFEVPSI
                     EKIVLNMGVGEAVTDTKKVTLAAADLGLIAGQKPVVTRARKAISTFKVRENMPIGAKV
                     TLRRTRMYEFLDRLITIALPRVRDFRGLNPKSFDGKGNYAMGVKEHIVFPEIDYDKAE
                     SILGLDVVVCTTAKSDDEARALLRAFNFPFRQ"
     gene            5397..5702
                     /gene="rpsN"
                     /locus_tag="VNY06_03335"
     CDS             5397..5702
                     /gene="rpsN"
                     /locus_tag="VNY06_03335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013652496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HWX13894.1"
                     /translation="MAKKSSIEKNKNRLRLVKQYAGRRRRLKAIANDESLTMEERFAA
                     RLKLAELPRNSAASRLRNRCEVSGRPRGFTRKVKMSRIALRELGSKGLIPGLVKSSW"
     gene            5714..6112
                     /gene="rpsH"
                     /locus_tag="VNY06_03340"
     CDS             5714..6112
                     /gene="rpsH"
                     /locus_tag="VNY06_03340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014890749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HWX13895.1"
                     /translation="MAMNDPLGDMLTRIRNAQMRRMGKVQTPGSRLRAHVLDVLQAEG
                     YIRGYSTTEYGNGRTEFEIELKYADGAPVIREIERVSKPGRRVYAAVGAMPRVANGLG
                     ITIVSTPKGVMADHAARDAHVGGEVLCKVF"
     gene            6137..6670
                     /gene="rplF"
                     /locus_tag="VNY06_03345"
     CDS             6137..6670
                     /gene="rplF"
                     /locus_tag="VNY06_03345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015928601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HWX13896.1"
                     /translation="MSRIGKKPVAIPAGVTAKVDGQSISVKGGKGELSFTAPDHVNVS
                     FSDGKIAVEPRNDSKAARAMWGMTRAMVNNLVSGVSKGFERKLEINGVGYRAAVSGKN
                     LQLSLGYSHDVSFPIPLGIAIATPKPTEVVITGIDKRKVGQTAAEIRALRPPEPYKGK
                     GIKYSDEFIFRKEGKKK"
     gene            6697..7059
                     /gene="rplR"
                     /locus_tag="VNY06_03350"
     CDS             6697..7059
                     /gene="rplR"
                     /locus_tag="VNY06_03350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018390616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HWX13897.1"
                     /translation="MAKNKSATSRRLARVRRSLRAHAYGKPRLSVFRSSCQIYCQVID
                     DSVGRTLAAASSLEKANRSSLKSGATVETAKIIGQQIAERALQAGISEVVFDRGAYMY
                     HGRVRALAESAREGGLKF"
     gene            7132..7698
                     /gene="rpsE"
                     /locus_tag="VNY06_03355"
     CDS             7132..7698
                     /gene="rpsE"
                     /locus_tag="VNY06_03355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012589605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HWX13898.1"
                     /translation="MAREGDGRSRDREDRDSEFMDRLVHINRVAKVVKGGRRFGFAAL
                     VVVGDQKGRVGYGHGKAREVPEAIRKATETAKRSLIRVPLREGRTLHHDVHGRHGAGR
                     VLLRAAPAGTGVIAGGPMRAIFEALGMHDVVAKSQGSSNPYNMVRATFDALRREDSPR
                     AIAGRRNLKVSVLQARRQGGETEATSEA"
     gene            7709..7915
                     /gene="rpmD"
                     /locus_tag="VNY06_03360"
     CDS             7709..7915
                     /gene="rpmD"
                     /locus_tag="VNY06_03360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018184922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HWX13899.1"
                     /translation="MTAKTAEKIVVEQVRSPIGRPASQRAVLIGLGLNKIGRRSSLED
                     TSAMRGMIAKVAHLIRIVEERQGG"
     gene            7936..8424
                     /gene="rplO"
                     /locus_tag="VNY06_03365"
     CDS             7936..8424
                     /gene="rplO"
                     /locus_tag="VNY06_03365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012384370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HWX13900.1"
                     /translation="MKLNELTDSPGASRSRMRVGRGIGSGKGKTCGRGVKGQKARTGV
                     VVKGFEGGQMPLHRRLPKRGFWNPFATLYNEVNLDRIQAAVDADKLDASAPVTIESLI
                     AAGVCAKPRDGVKILGQGALTVILAFEVAAASKNAVAAIESAGGSIVLLAKQAQDQAS
                     ET"
BASE COUNT         1984 a         2278 c         2656 g         1517 t
ORIGIN      
        1 aaaaagctgc gcgtgctcgc cctcaaacat gcgctttccg ccaaagcaaa ggaaggccaa
       61 atcatggtct ttgccgaggt gaggtcgaca gacgccaaga ccaaatcttt gagaacgagc
      121 ttgcgaaagc tcggcctcac caatgcgctg attatagacg gcactgaggt ggcgacgaac
      181 ttccagcttg cagcgcgcaa cattccgcaa atcgacatcc taccggtgca ggggatcaat
      241 gtctacgata ttatccgccg cgagaagctc gtcttgacca aagccgcgat cgaagtcttg
      301 gaggcgcgtt tcaaatgagc ccttccaaac tcggccatga cagccagcat tacgagatca
      361 ttctcgcccc ggttatcact gagaaggcaa cgatggcgtc ggaggcaaat caggtcatct
      421 tcaaggttcg aaaggacgca acgaagccac agatcaaggc ggcagtcgaa cgtctctttg
      481 acgtcaaagt ggcagccgtt aacacgctca tccgcaaggg caagcgcaaa ttgtttaaag
      541 gcacacgcgg cgtccaatcc gacgtgaaaa aagccgtcgt gaccctcgct gagggtcata
      601 agatcgacgt aaccacaggg ctctgagccg atgcgaggac gacaccaatg gcactgaaaa
      661 ctttcaagcc ggttacgcca agccttcgcc aggtcgtgat tgtcgatcgc acgggccttt
      721 ataaaggcaa gcccctcaag cagctaaccg aaggcaagtc gagttccgga ggacgaaaca
      781 acaacggccg tgtcaccgta cggtttcgcg gcggcggcca caaacagagt taccgcatcg
      841 tagatttcaa gcggcgcaag cttggtgtgg ccgccaaggt cgagcggatc gaatatgatc
      901 cgaaccggac cgcctttatc gcgctcatcc gctacgccga tggcgaacac gcttatattc
      961 tggcgccgca gcggctcgcc gcgggggatg aggtgatcgc cggcctgcag gccgatgtga
     1021 agccggggaa cgcgatggcg ctcgccaata ttcccgtcgg cactattgtc cataatatcg
     1081 agatgaagat cggcaagggc gccgccatcg cgcgtgcggc gggcagctat gcccaagtcg
     1141 ttggccgcga tgaaggctat gtcatcatgc gtctcacctc gggcgaacag cgactgatcc
     1201 acggccagtg cttcgcgacc atcggggccg tctcgaatcc cgatcacatg aatatctcgc
     1261 atggcaaggc tggacgaagc cgctggttgg ggcaaaggcc gcataaccgg ggtgtgacga
     1321 tgaatccggt ggaccatccg catggcgggg gcgagggccg cacttcggga ggacgccatc
     1381 cggtgacgcc ctggggcaaa ccgaccaaag gcaagaaaac caggtctaac aagtccacca
     1441 ccaaattcat cgtgacctcg cgtcacaaga gcaagaagaa gggctaatca tggcgcgctc
     1501 gatctggaag ggcccgtttg tcgacggcta tctgctcaaa aaggcggaaa cggcgcgcaa
     1561 ttccggccgg tccgaaatca tcaaaatgtg gagccgccga tcgacgatat tgccgcagtt
     1621 cgtagggctc accttcgggg tctacaatgg ccacaagcac atccccgttt ccgtgaccga
     1681 ggacatgatc ggccataaat ttggtgagtt ttctccgacc cggaccttcc atggtcatgc
     1741 cggggacaaa aaagccaaac gaggctaagc gagagcgatt atgggcaaag agcaagcgcc
     1801 gcgcgcgcta aaggaaaacg aagccaaggc ggtcgcaagg atgctgaggg ttagcccaca
     1861 gaagatgaat ttgcttgcgc aattgatccg cggcaagaag gtcgataagg cgctcgccga
     1921 tctcgcgttt gcccgcaagc gcagtgcgct cgacgtgaaa aaaacactgg agagtgcgat
     1981 tgccaatgcc gagaacaatc acaacctcga tgttgacgat cttgtcgtcg ctcaggccta
     2041 tgtcggcaat gcgatgatcc taaagcggtt cagcgcgcgg gcgagggggc gctccggccg
     2101 cctccgcaag cctttttcgc atctgacaat catcgtccgg gaaaccggcg ccgacgcctc
     2161 tcaggcgtga ggagaaagag atgggacaaa aagtcaatcc gatcggctta cggctcggcg
     2221 tcaacaggac ctgggattcg cgctggttcg cgggcaaggc cgaatatggg aagcttttgc
     2281 acgaggacat ggcaattcgc gccacactca tgaaaaacct caagcaggcg gcgatctcaa
     2341 agattattat cgagaggccg cataagaaat gccgagtgac aatccattcc gcacgccctg
     2401 gcgtggtgat cggcaagaaa ggcgcggaca tcgacaagat ccgcaagacc atcacgaaga
     2461 tgaccgactc cgaggtggtc atcaatatag tcgaggtgcg taagcccgaa atcgacgcgg
     2521 tcctcgttgc cgattcgatt gcccagcaat tggagcgccg cgttgctttc cgccgggcca
     2581 tgaagcgggc ggtgcagtcc gcgatcaggc taggtgccga gggcgttcgg atcaattgct
     2641 cgggccgcct cggcggggct gaaatcgcga ggctcgaatg gtatcgcgaa ggccgcgtgc
     2701 ccctgcatac gctgcgcgct gatgtcgatt acggcaccgc gacggcacat accgcctatg
     2761 gcacctgcgg aatcaaggtc tggattttca aaggggaaat cctcgaacat gatccgatgg
     2821 cgcaggacaa gaagctgacc gagcaggacc gcactggcgg cgagcggcgg cgccgcgacc
     2881 gcgatgccgc ctgaaccttc ggaataggaa ttacgagtct taagccatgt tgcaaccgaa
     2941 gcggacaaaa ttccggaaag cctttaaggg ccgtatcagc ggtgctgcga aggcagggtt
     3001 cgaactgaat tatggccagt tcgggctgaa ggcgctcgag cctgagcgga ttaccgcgcg
     3061 ccagatcgag gccgcgcggc gcgcgttgac ccgtcacatg cggcgcgcgg gccgcgtatg
     3121 gatcaggatt tttccggatg ttccggtctc gaaaaagccg accgaggtgc gcatgggcaa
     3181 gggcaaaggc gcaccagaat attgggtgtg ccgcgttgca ccgggccgca tcatgttcga
     3241 gctcgatagc gtgtcagctg atctggccca ggaggcgctt cgtctcgctg cagccaagct
     3301 gcccataaag acacgctttg ttcagcgcat tgtcgaatag ggcggaaccg tgaagcagag
     3361 acagcgcttg tcagacctta aggtaatgtc caacgatcag cttgagcaag agctcctgaa
     3421 ccttaagaag gagcagttta acctgcgctt ccagcgggcg acagggcaac tcgaaaacac
     3481 ggcgagggtg cgtacggtgc gccgcgatat cgcaaggata aaatcgcttt cggcgcaaaa
     3541 gcggcgcgaa ggcgccaaat tgaaacagga atgaggctga gaagaggttt tagatatcat
     3601 gccgaggcga attcttcaag gtgtcgtggt aagtgacaag caagacaaga cgattgtcgt
     3661 caaggtcgag cgccgtttca cgcatccgct gctgaagaaa acggtgcggc gctcaaaaaa
     3721 atatcatgcg catgacgaaa cgaacgcgca taaggccggg gatgcagtct caatcgaaga
     3781 gacgaaaccg atctcgaagt tgaagcgctg gagtgtcgtt aaagtggcgg gcaaataagg
     3841 atttaaggtt agttgagagg gacggcctcc atcaggtcgc ggcccagcgc agcgcaagag
     3901 cgaacggaaa taaggtgcaa gacgatgatt cagatgcaga ccaacctcga cgtcgccgat
     3961 aattctggcg cgcgccgtgt catgtgcatc aaagtgctcg gcggctcgaa acgcaaatat
     4021 gccggggttg gtgacacgat cgtcgtttcg atcaaggagg cgatcccacg cggacgcgtc
     4081 aaaaagggcg atgtgatgaa ggcagtcgtt gtgcgcaccg ctaaggacat caagcgtcct
     4141 gacggctccg tcataaggtt cgattccaat gcggcggtgt tgctcaacaa tcagtcggag
     4201 ccggttggca ctcgcatctt tgggccggtt ccgcgcgaac tccgcgcaag aaatcatatg
     4261 aagatcatct cccttgcgcc agaggtgttg tgatggcggc aaaaatcaag aagggggacc
     4321 gggttgttgt cctcgctggg cgcgacaagg gccgcgcggg ggaagtggtt ggcgttgccc
     4381 caagcgagga acgggccatt gtgcgcgggc tcaatatggt gcagcgacat caccggcaaa
     4441 ccgcaaagca ggaagggggc atcgtctcga aggaggctcc gatccatttg tccaatctgg
     4501 cgattgccga tcccaagaac ggcaagccaa cgcgagtggg atttaagact ttgccggatg
     4561 gccgaaaggt ccggttcgca aagcgttctg gggaattgat cgatggctga ggcgaaaacc
     4621 ggcaaggacg ggtccaaggg gccgggtaag tccgcggcca aggttagcgc caaaggctct
     4681 cctaaggaca gtccaaaggg tgctgcgaaa gaagcggcga agtcaggcaa gtcggcgaag
     4741 gcgccggctg gccacggcag agaagacggg gcgcgtgccg ccccggcgaa agactttgcg
     4801 ccgccaaccg attacgcccc gcggctcaaa aaatattacg ccgacgtcgt gcagccccgg
     4861 ctcattgagc aattcggcta caagaaccgg ttcgaagtgc caagcatcga aaagatcgtg
     4921 ctaaatatgg gggtgggtga agcggttacc gacacgaaga aggtcacgct cgccgccgcc
     4981 gatcttggtt tgattgcggg gcagaagccg gtcgtcacgc gcgcgcggaa ggcgatctcg
     5041 accttcaaag ttcgcgagaa tatgccgatt ggcgccaagg ttacattgcg ccggacgcgg
     5101 atgtatgaat tcctcgatcg cctcatcacg atcgcgttgc cacgggtgcg ggattttcgc
     5161 ggcctcaacc cgaagagctt cgacggcaag ggcaattatg cgatgggggt caaggaacat
     5221 atcgttttcc ctgaaatcga ctatgacaag gctgaaagca ttttgggtct cgatgtggtc
     5281 gtgtgcacga cggcgaaatc cgatgacgag gcgcgcgcat tgttgcgtgc cttcaacttc
     5341 ccgttccggc agtgaagcgg caacggttca aacgcggata ccagaggtaa agatagatgg
     5401 cgaagaaaag ttcgattgag aaaaacaaga accgcttacg gctggtcaag caatatgctg
     5461 gccgccggcg ccgcttgaaa gcgatcgcca atgacgaaag cctgaccatg gaggaacggt
     5521 tcgccgcgcg tctgaagctt gctgaacttc cgcgcaattc cgccgcttcg cgtcttcgta
     5581 accgctgcga ggtgagcggc cgcccgcgtg gctttacccg caaagtaaaa atgtcgcgca
     5641 tcgcactccg ggaattgggg tcgaagggct tgatccccgg cctcgtgaag tcgagctggt
     5701 aggaggtcgg atcatggcga tgaatgatcc cttgggcgat atgctcaccc gcatccgcaa
     5761 tgctcagatg cggcgcatgg ggaaagtgca gacgccgggc tcgcgccttc gcgcgcatgt
     5821 tctcgatgtc cttcaggcgg aagggtatat tcgtggctat tctacaacag aatatggcaa
     5881 cggccggacc gagttcgaaa tcgaattgaa atacgccgac ggcgctccgg tcattcgcga
     5941 aatcgagcgg gtatcgaagc caggccgccg cgtctatgcc gcggtgggag ctatgccgcg
     6001 cgttgcaaat gggctgggaa tcaccattgt ttcaactccc aaaggcgtga tggccgacca
     6061 cgccgctcgg gatgctcatg tcggcggtga agtcctctgt aaggtctttt agccggcgga
     6121 atgaggaact ctcagaatgt ctcgtatcgg aaagaaaccg gttgcgattc ctgccggagt
     6181 cacggccaag gtcgacggcc aatccatctc ggtgaaaggt ggcaagggcg agctatcctt
     6241 caccgcgccc gatcacgtga acgtgagttt cagcgatggc aagatcgccg tggagccgcg
     6301 caacgacagc aaagctgcgc gggcgatgtg gggcatgacg cgtgcgatgg tcaacaatct
     6361 cgtttccggc gtttcaaaag gatttgaacg caagctcgag atcaatgggg tcggctaccg
     6421 ggccgcggtt tcgggaaaaa acttgcagct ttcgctgggt tatagtcatg atgtgagttt
     6481 tccgatcccc ttgggaattg caattgcgac gccgaagccg acggaagtcg taatcaccgg
     6541 catcgataaa cggaaagtgg ggcagaccgc ggcggaaatc cgcgccttgc gcccacccga
     6601 accttacaaa ggtaagggga tcaaatattc tgacgaattc atcttccgta aggaaggaaa
     6661 gaagaagtag aagagacgaa agcgaggatt gagtgcatgg cgaaaaataa gagtgcgacc
     6721 tcccgcaggc tggctcgcgt ccggcggtcg ttgcgggcgc atgcctatgg caagccgcgg
     6781 ctgagcgtct tccgttcgtc ctgccagatc tattgccaag tcattgacga tagcgtgggc
     6841 cgcacgcttg ccgccgcctc gtcgctggag aaagccaacc gcagcagtct gaaatcgggt
     6901 gcgaccgtgg agactgcgaa gattatcgga cagcagattg ccgagcgcgc cctgcaggct
     6961 ggaataagcg aagtggtgtt cgaccggggc gcctatatgt atcacgggcg tgtcagggct
     7021 cttgccgaaa gcgcgcgcga aggcgggctt aagttttaga ccaagcagca gagagcattc
     7081 tgccaatgcg cgggaacaat tacaaggtag actggacaat ggaacggacc catggcgcgt
     7141 gaaggagacg gccgtagccg cgatcgcgag gaccgcgaca gcgaattcat ggatcgtttg
     7201 gtccacatca accgcgttgc aaaggtggtg aaaggcggcc gcagatttgg ctttgcggcg
     7261 cttgtcgttg tcggcgatca gaagggccgc gtcggttatg gccatggcaa ggcgcgcgag
     7321 gtgcctgaag cgattcgcaa ggcgaccgaa actgcgaagc gctcccttat ccgggttccg
     7381 ttgcgggagg gccgcacgct gcaccatgac gtccatggac ggcatggggc ggggcgtgtc
     7441 ctgttgcgtg cggctccagc gggaactggc gtcatcgccg gcggcccgat gcgcgctatc
     7501 ttcgaggccc ttggcatgca tgatgtcgtt gccaagtccc aaggatcctc aaacccgtac
     7561 aatatggtgc gggcgacttt cgatgccttg agacgggagg attccccgcg tgccattgcc
     7621 gggcgccgta acctgaaggt ttccgttctt caggcccgcc gccagggagg cgagacagaa
     7681 gctaccagcg aagcatgagg agcacagcat gacggcaaag accgcggaga aaattgtcgt
     7741 ggaacaggtc aggagcccga tcggccgtcc cgcctcgcag cgcgcggtgc ttattggtct
     7801 cgggctaaac aagataggac ggcgctcctc gcttgaagat accagtgcga tgcgcggcat
     7861 gatcgcgaaa gttgcccatc tcattcgcat tgttgaagag cggcaaggcg gctgagacca
     7921 tttgaggacg cgatgatgaa gctcaacgaa ttaacggata gtcctggcgc ctcgcgttcg
     7981 cgcatgcggg tcggccgggg gatcgggtcc ggcaagggca agacctgcgg acgcggtgtc
     8041 aaaggtcaaa aagcgcgcac tggggtcgtg gtcaaagggt tcgagggcgg ccagatgcca
     8101 ctgcaccggc ggctgccaaa acgcggtttc tggaatccct ttgcaaccct ctacaatgaa
     8161 gtcaatcttg accgaatcca ggcggccgtg gatgcagaca agctcgatgc ctcggcaccg
     8221 gttacgatcg agtcgctgat cgccgcaggc gtttgcgcca aaccgcgcga tggggtgaaa
     8281 attctcgggc aaggagcgct gacggtaata ctcgcgttcg aagtagccgc ggcatcgaaa
     8341 aacgctgtgg ccgcaatcga gagcgcgggc ggatcgattg tactgctcgc taagcaagcc
     8401 caagatcaag cgagcgaaac ataagtggac cggca
//
LOCUS       DATMPU010000144         2102 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_2912799,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000144 DATMPU010000000
VERSION     DATMPU010000144.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 2102)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2102)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2102
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2912799"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..195)
                     /locus_tag="VNY06_03370"
     CDS             complement(<1..195)
                     /locus_tag="VNY06_03370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011315659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HWX13901.1"
                     /translation="MQLSNLAASWQPRLLSLLRIITALLLLQHGTAKLLGFPHVAMFD
                     GLKLFSLLGVAGILEFGGGVL"
     gene            384..1616
                     /locus_tag="VNY06_03375"
     CDS             384..1616
                     /locus_tag="VNY06_03375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012591346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HWX13902.1"
                     /translation="MHAIAVAVVSALVLSGALGIFLLCRQAAAVAAHCESVPAGFQGE
                     ISLEEHQRAAAYGSASARLGIAKTVFDTILAALWLTILLGPLYLLVAQLIPPGISRSV
                     AIVVAFALIERLFHLPFSVFSTFWLEARFGFNRATPLMFIRDRIKGLLLSCALGVPLL
                     FGLFWLPGVLPNSWWLAAWAALIALAIAMSVIFPVIIDPMFNKFMALPDGPLRVRIEA
                     LLGKCGFASNGLYVMDASRRSTHGNAYFTGFGKAKRIVLFDTLLQNHTQDEILSILAH
                     EVGHYKLGHIGQRIAETAAYAFLGFAVLGFTFANGRLAGAFGLPSDPGLILIVVLTAM
                     GPFLHLLSPLTSYLSRRAEYQADNFARAMTGPSPMASALTRLARDNLSTLTPDWLYAL
                     FYCSHPPVPARIARLRGT"
     gene            complement(1646..1822)
                     /locus_tag="VNY06_03380"
     CDS             complement(1646..1822)
                     /locus_tag="VNY06_03380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX13903.1"
                     /translation="MPAYAKGLLPWSVVSRPLEGEAPTIDVAVGYNRSSASPILRLFL
                     SRLDELTGPTNQKG"
     gene            complement(1822..>2102)
                     /locus_tag="VNY06_03385"
     CDS             complement(1822..>2102)
                     /locus_tag="VNY06_03385"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HWX13904.1"
                     /translation="AFLRVEPGYDLEYHVVDREPLIVLMPSDHRLTAREAVRPQDFVG
                     EIFIGGSNKAAVLRTVTEDYLRRTSSWITGWTIWRWPCRWSRPPAGWR"
BASE COUNT          383 a          640 c          588 g          491 t
ORIGIN      
        1 cagcacgccg cctccaaact ccagaattcc ggcaacgcct aagagcgaaa agagctttag
       61 cccgtcgaac atggcgacat gcggaaagcc aagcaatttc gccgtcccat gctgaagcag
      121 caaaagagcg gtgatgatcc gtaaaaggct gagaagccgc ggctgccagc tggcagcgag
      181 gttcgaaagc tgcatgacga aaaatccttg tcccttgcgg ttcgaaaccc aaccaacgag
      241 agcgagatgg cttttcttgc aatcgtccta ttgctctaga tctttgtttt gtcgcttgtc
      301 tggacgcgaa acctacccac ttttgcggga cacgccctaa gcttatataa taaggcaatg
      361 ccgcgttttt ctggactagt cggatgcatg cgatagccgt tgccgttgtt tctgctcttg
      421 tcttgtccgg tgcgctcggg atttttctgc tttgccggca ggccgccgcc gttgccgcgc
      481 attgcgagag cgttcccgct ggttttcaag gcgaaatcag cctcgaggag catcaacggg
      541 cggccgcata cggttcggcc agcgcgcggc ttgggatcgc caagaccgtc ttcgacacca
      601 tccttgccgc gctatggctc accatccttc ttgggccgct ttaccttctg gtagcacaac
      661 tcattcctcc cggcatctcc cgcagcgttg cgatcgtggt tgcctttgcg ctgatcgagc
      721 ggctgttcca tctgccattt tctgttttca gcaccttttg gctggaagcg cgtttcggct
      781 ttaatcgcgc aacccctttg atgttcatac gcgaccggat caagggtttg ctgctatctt
      841 gcgcgctcgg cgtgccattg ctttttggcc tcttttggct gccaggcgtg ctgcccaact
      901 cttggtggct tgccgcatgg gcggccctca tagctctggc aattgcaatg agcgtgattt
      961 ttccagtcat tatcgatccg atgttcaata agttcatggc cctgccagac ggaccgttga
     1021 gagtgcgtat cgaggcgctt cttggcaaat gcggatttgc atcaaacggg ctctatgtca
     1081 tggacgcgtc gcggcgttcg acgcatggca atgcctattt taccggattt ggaaaagcca
     1141 agcgcatcgt ccttttcgat acgcttttgc aaaatcacac ccaagacgaa atcctgtcga
     1201 tccttgccca tgaagtcggc cactataagc tcggacatat tggccagcgg atcgcggaaa
     1261 ctgcggctta tgcgtttttg ggcttcgctg tgctcggctt taccttcgcg aacggcagac
     1321 tagctggcgc attcggcctg ccctccgatc ctggtttgat tttgatcgtc gtcctgaccg
     1381 cgatggggcc tttcttacat ctcctgtcgc cgctgacgag ctacctctcg cgccgggccg
     1441 aatatcaggc cgacaatttc gctcgagcga tgaccgggcc ttcgccaatg gccagcgcct
     1501 tgacgcggct cgcccgcgat aatctttcga cgctcacgcc agactggctt tatgcattgt
     1561 tttattgttc ccatccccct gtgccagcgc ggatcgcccg attgagaggc acctgacgcc
     1621 ccccaggctg tgcaaaaacg tggggtcagc ccttctggtt cgtcgggccc gtcaattcgt
     1681 cgagccgcga caggaacagc ctgagaatcg gcgaggcgct cgacctgttg tagcccaccg
     1741 ccacatcgat cgtcggtgcc tcgccttcca gcgggcggct gacgacggac cacggcagca
     1801 agcccttggc gtaggcgggc atcagcgcca gcccgcgggt ggacgggacc agcgacatgg
     1861 ccatcgccat attgtccacc ccgtgatcca gcttgaggtc cggcgcaaat agtcctcggt
     1921 gacggtgcgc agcacggcgg ccttgttcga accgccgatg aaaatttcgc cgacgaaatc
     1981 ctgcgggcgc acggcttcgc gtgccgtgag gcgatggtcg ctcggcatca ggacgatgag
     2041 cggctcccga tccacgacgt gatactcgag gtcgtagcca ggctccacgc gcaggaaggc
     2101 ca
//
LOCUS       DATMPU010000145         4069 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_1056412,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000145 DATMPU010000000
VERSION     DATMPU010000145.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 4069)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4069)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4069
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1056412"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..868
                     /locus_tag="VNY06_03390"
     CDS             <1..868
                     /locus_tag="VNY06_03390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012496272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HWX13905.1"
                     /translation="PARFDRIEGLEHLDKVIDIDQSPIGRTPRSNPATYTGAFTPIRE
                     WFAGLPESKARGYEPGRFSFNVKGGRCEACQGDGVIKIEMHFLPDVYVTCDVCKGKRY
                     NRETLEVLFKGKSIADVLDMTVEEALDFFKAVPRVRDILTLLRRVGLDYIHVGQQATT
                     LSGGEAQRVKLAKELSKRATGRTLYILDEPTTGLHFHDVAKLLEVLHELVESGNTVVV
                     IEHNLEVLKTADWIIDLGPEGGDGGGEIVATGTPEDIAAVKRSYTGQFLKPVLARGEA
                     GGKRKRRIEAAE"
     gene            924..1541
                     /locus_tag="VNY06_03395"
     CDS             924..1541
                     /locus_tag="VNY06_03395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX13906.1"
                     /translation="MPLLAFWESNPEEVGKSSIEQIVAMAGDGNLKDGSSCSDELRKY
                     LAEAESAKLGTYIEHCLESGFSKSGYVFQDLVNELGRRLDYKVTNGRYQGTVSEIGHD
                     GIWVSPEGQTIIAEVKTTDAYRIPLDTIAGYRQKLLAANKTASPSSILILVGRQDTGE
                     LEQQIRGSRHAWDIRLISADALMKLVQLKENSEDSETGGAKFAAF"
     gene            1583..2140
                     /locus_tag="VNY06_03400"
     CDS             1583..2140
                     /locus_tag="VNY06_03400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX13907.1"
                     /translation="MFTAATDVETASPEFVDGDKTKAVPTSKDIIVAPSGWEFTDSVI
                     LDKKRADIIDAMSRKTKTKLIKKSRALFWDATHEVRIACSISKRYPKSYLYWYAFHPE
                     WREFLNEGRESYFVLGCMDLKLAFAIPLKVIAANLDALNTTTTSKKTYWHIHIVESAS
                     GGYAVPLRHGKQLSIDTYQIPIEQM"
     gene            2330..2470
                     /locus_tag="VNY06_03405"
     CDS             2330..2470
                     /locus_tag="VNY06_03405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX13908.1"
                     /translation="MPAADLYPYVYVNADGTARELHPSEQKYLETEFKGGGGAAPYVK
                     RA"
     gene            2796..3449
                     /locus_tag="VNY06_03410"
     CDS             2796..3449
                     /locus_tag="VNY06_03410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018042766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HWX13909.1"
                     /translation="MAEPGAPAAAFPKPTRPVAAIVSPIWHDEDERDAAGESDQLIRL
                     LGIKSGMTVADIGAGSGYYVVRLSPIVGAGGRVIAEDVVPVYLRRLRERVRDLELQNV
                     TIGLGEPHDPRLPPDSVDVAILVHMYHEIAQPYALLYNLVPALKPGARVGIVDAFRPT
                     AEHGTPPSVLRCELAAVGYREISLDRLGGANAYLAIFAPPSVASRTRPEAIAACKSL"
     gene            3946..>4069
                     /locus_tag="VNY06_03415"
     CDS             3946..>4069
                     /locus_tag="VNY06_03415"
                     /inference="COORDINATES: protein motif:HMM:NF024750.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX13910.1"
                     /translation="MTPRGRKVFIVMYRLGGAGSRLRKYTIGPYGRITLPMARAQ"
BASE COUNT          885 a         1196 c         1190 g          798 t
ORIGIN      
        1 ccctgcccgg ttcgaccgca tcgaaggcct cgagcacctc gacaaggtga tcgacatcga
       61 ccagtcgccg atcggccgca cgccgcgctc gaacccggcg acctataccg gcgcgttcac
      121 gccgatccgc gaatggttcg caggcctgcc ggaatcgaag gcgcgcggct acgagcccgg
      181 ccgcttctcc ttcaacgtca agggcgggcg ctgcgaagcc tgccagggcg acggcgtcat
      241 caagatcgag atgcactttc tgcccgacgt ctatgtcacc tgcgacgtct gcaaggggaa
      301 gcgctacaac cgcgagacct tggaggtgct gttcaaagga aaatcgatcg ccgacgtgct
      361 cgacatgacc gtcgaggagg cgctcgactt cttcaaggcg gtgccgcggg tgcgcgacat
      421 cctcacgctc ctgcgccgcg tcggcctcga ctacatccac gtcggccagc aggcgacgac
      481 gctctccggc ggcgaggcgc aacgggtaaa actcgccaag gaactgtcga agcgcgccac
      541 cggccgcacc ctctacatcc tcgacgagcc gaccaccggc ctgcacttcc acgacgtcgc
      601 caaactcctc gaggtgctgc acgagctggt cgagagcggc aacacggtgg tggtgatcga
      661 gcacaacctc gaagttttga agaccgccga ctggatcatc gacctcggcc ccgaaggcgg
      721 cgacggcggc ggcgagatcg tcgccaccgg cacgccggaa gacatcgccg ccgtcaagcg
      781 cagctatacc gggcagttcc tcaagccggt gctggcgcgc ggcgaggccg gcgggaagcg
      841 caagcggcgg atcgaggcgg cggagtaagt gccctgggtt gctatactat tatgaaattc
      901 gtcgcatcaa tgccggacgt gccatgcccc ttttagcctt ttgggagtca aatcctgagg
      961 aagtaggcaa atcctcgatc gagcagatcg tggcaatggc aggggacgga aatctcaagg
     1021 atggcagctc ctgctcggac gaattgcgca aatatttggc tgaagcggaa tctgcgaaac
     1081 taggaacgta cattgagcat tgcctagaat ctggtttttc gaaaagcgga tacgtttttc
     1141 aagatcttgt aaacgaactc ggtcgaaggc tggattacaa ggtaacaaac ggccgttatc
     1201 aaggcacggt tagcgaaatc ggacatgacg gcatctgggt atcacccgaa ggccagacga
     1261 tcatagccga ggtcaaaacg acagacgcct accggattcc gctggataca atcgcggggt
     1321 accgtcaaaa gcttctggcc gctaacaaaa cagccagccc gtcatcaatt ttaatccttg
     1381 ttggcagaca ggataccggt gaactcgaac aacaaattcg cggatcgaga cacgcttggg
     1441 acatacgctt gatcagcgca gacgctctca tgaaattagt tcaactaaag gagaattccg
     1501 aagattcaga aactggagga gcaaaattcg cagccttttg actccgttag aattcactcg
     1561 gttagatggg cttgtcgatg tcatgttcac ggcggcaaca gatgtcgaaa ccgcttcgcc
     1621 ggaatttgta gacggagaca agacaaaagc cgttcctact tcgaaagata ttattgttgc
     1681 accaagcgga tgggaattta ccgactccgt gatccttgac aagaagaggg ccgatataat
     1741 cgatgcaatg tcgcgcaaga cgaaaacgaa attgatcaaa aaaagtcgtg ctttattttg
     1801 ggatgcgacg catgaggtac gaattgcttg ctcgatctca aagcgatacc caaaatctta
     1861 tctttactgg tatgcgtttc atcctgaatg gcgcgagttc ctaaacgaag gaagggagtc
     1921 gtactttgtg cttggttgca tggatttaaa actggcgttt gctattccac ttaaagtgat
     1981 tgctgcaaat cttgacgctt taaacacgac gacaaccagc aagaaaactt attggcacat
     2041 tcacattgtg gaaagtgcca gcggcggcta cgccgtgccc ttgcgtcatg gcaagcaact
     2101 gtccatcgat acataccaaa tcccgatcga gcaaatgtag ctctcccctt ggtgtgtcac
     2161 caccgggctt ggcccgacga ccgtgagatt tcccagccgc gtgcattttc gggcaagcaa
     2221 gcgtagcccg gatggagcgc agcgcagtcc ggggagcggc cgaagaggcc ttgtgccaga
     2281 cctggatttc gcttcgcttc atccgggcta cagctgccgg tgtccatgca tgccagccgc
     2341 cgatctctat ccgtacgtct atgtcaatgc cgacggcacg gcccgcgaac tccatccgag
     2401 cgagcaaaaa tatctcgaaa ccgagttcaa gggcggcggt ggcgccgcgc cttatgtgaa
     2461 gcgagcgtag ggtgggcaaa ggcgcaacgc gccgtgccca cgtagtgaaa ccgcgtgggc
     2521 ttcgctgcgc tcagcccacc ctacatattc gtctgatcgg taggagcaag tgagcgacgg
     2581 taaaggttga aaccccaggc ggcgccaagg cctatcaagt ccagaaaggt ggcttcgagc
     2641 acccgatgat cgatgcgcta caagcattca aatcggcaca ttggccggca gcggacggct
     2701 tgcggccgtg gacgatgatg cgacattgca aggcgcttgc cgctgttttt gccgtcttgt
     2761 tcgcgacatc aagcgcgggc tttgcccaag acttcatggc ggagcccggc gcgccggctg
     2821 cggcttttcc gaaacccact cgtccggtcg ctgctatcgt cagccccatc tggcacgacg
     2881 aagacgagcg cgacgccgcc ggcgaatccg accagctcat acgtctgctt ggcatcaaat
     2941 ccgggatgac ggtagcggac atcggcgccg gcagcggcta ctacgtcgta cggctctccc
     3001 cgatcgtcgg cgccggcggc cgcgttatcg cagaagacgt tgtgccggta tatttgcggc
     3061 ggctgcgcga gcgggtgcgc gatctcgaat tgcagaacgt caccatcggc ctcggtgaac
     3121 cgcatgatcc gaggttgccg ccggattcag tcgatgttgc gatcctggtt cacatgtatc
     3181 atgagatcgc tcaaccctac gccttgcttt acaacctcgt gcccgcgctc aagccgggcg
     3241 cgcgtgtcgg aattgtcgac gctttccggc cgacggccga gcacggcacg ccgcccagcg
     3301 tcctgcgatg cgagcttgca gccgtggggt atcgtgagat cagcctcgat cggctcggcg
     3361 gcgccaacgc ctatctggcg atcttcgcgc caccctccgt tgcaagccgc acgcggccgg
     3421 aggccatcgc cgcatgcaaa tcgctatagc agcggcaaac caacgcgagg cccctgcacc
     3481 tcggccgcat ccttcgaggc tcgctgccgc tcgcacctca ggacgacggt gtacgttagt
     3541 cacattttca ttgacactcg tccccgttta tgttaccggc acaatgcccg ctcatccgag
     3601 ggacgtcacc ggtgacggcc gattgacgga gcgggtgcgg ttcctgcgcg cggggctcgt
     3661 aaccccgttc tcgggcggac tcggtaatgg cgttcccgca gattgacgat gatggccaag
     3721 cattgattta tcgccatatc ttccgcaggg atagtggatg tggatggccg gtgatcgccc
     3781 ttgtttcccc ttcagtgtta cctatgtgaa ctcaaggtag cacgggaaat aggggaacaa
     3841 caccattcct cgcgtccata tgaccaagtc ggcgattgac ggatttgccg tcgccgaaag
     3901 gatacagtgt actgggatca aggactgccg ggctttggcg tcaaggtgac gccgaggggc
     3961 cgcaaggtct tcatcgtaat gtatcggctt ggcggcgccg gctcacgatt gcgcaaatat
     4021 accatcggtc cctacggacg catcaccttg ccgatggcgc gtgcccagg
//
LOCUS       DATMPU010000146         6543 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_2285984,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000146 DATMPU010000000
VERSION     DATMPU010000146.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 6543)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6543)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6543
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2285984"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            139..639
                     /locus_tag="VNY06_03420"
     CDS             139..639
                     /locus_tag="VNY06_03420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015928071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HWX13911.1"
                     /translation="MSLLQIELDRTEHPVDIIERIAALNHWPFDRGEQDEISISVAGG
                     WTQYTVAFTWLAEVEVVHVACAFDLKIPETRRLELASLVSLINEELWIGHFDIWPTDG
                     VVMFRHAILLSGGAELNSQQCQSALARIVRACERYYQAFQFVVWAGKAGREALATVML
                     ETQGRA"
     gene            641..1468
                     /gene="proC"
                     /locus_tag="VNY06_03425"
     CDS             641..1468
                     /gene="proC"
                     /locus_tag="VNY06_03425"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012384256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HWX13912.1"
                     /translation="MESAEADRFNLSLMLAGAGKMGGAMLRAWLDQGYDPRQISVLEP
                     HPSQEVVSLARAKGFVLGCLSEPPQVLVLAMKPQSFDEAATGLESFADQGTLIISILA
                     GKTFANIASRLPRASAIIRAMPNLPAAIGRGITALAANPAVTPVQRSAAEALLGATGR
                     VEWLAGEHLIDAAAAVSGSGPAYVFYLAEVLAEAGEALGLPADISARLARGTIEGAGE
                     LLFQSPGSTVAELRENVTSPGGTTAAALEILMSEEGLAPVIQRALRAAKRRAEALSG"
     gene            2065..2844
                     /locus_tag="VNY06_03430"
     CDS             2065..2844
                     /locus_tag="VNY06_03430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020174234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HWX13913.1"
                     /translation="MGAAQEVNMADESSGKDEARAEAGPDCKTIAAIAKDPRNAIIDA
                     LLELASERSWEDITISDVASRANVSLSAFRDYFPSKGAVLASFSRRIDHVVLDATGDD
                     LAGEPVKDRLFDILMRRLDALAPYKLGLEAVYEWARRDPLAASALNRVVVNSMRFMLE
                     AAGIDSEGPVGALKLQGLALAWARILRVWFQDDDPGLASTMAALDRELTRGSSFVARA
                     EDLNRLVSPLFSIARSLLESRPSRRQSRPGGLGDRENERSR"
     gene            complement(2943..3479)
                     /locus_tag="VNY06_03435"
     CDS             complement(2943..3479)
                     /locus_tag="VNY06_03435"
                     /inference="COORDINATES: protein motif:HMM:NF008528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FxsA family protein"
                     /protein_id="HWX13914.1"
                     /translation="MVTLMLRLRSDGRFVHRPLLRLPVLIVTYFALWLGAELAAFGAV
                     VHAIGFVGAILLCILTSLAGLTMLRRMGVSAALKLRQVAVKNTTERNGLTKDKLLDGT
                     LAGLGAVLLILPGFVSDLVGLALAAPSFRLWVSERLSLSRLGRHGGRPQTPALINLTP
                     REWSRQEEQKSNTKETLP"
     gene            3577..4296
                     /locus_tag="VNY06_03440"
     CDS             3577..4296
                     /locus_tag="VNY06_03440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009490669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tim44/TimA family putative adaptor protein"
                     /protein_id="HWX13915.1"
                     /translation="MQEPFDITTIIFALLAAFVVWKLRSVLGTRNGTEKPPRNPFATR
                     APGGFENRSGDATPQAIPLPGAAETPISATPVKDEKWKPYAEPGSSAWAGLDSVAAAD
                     PSFAIQPFAAGAKLAYEMIILAFAEGNRKVLRNLLSPDVYEGFAAALDERERRGEKLS
                     TTFVSIDGMTIDDATLVGRTAKIKVHFTSQMITATRDANGTVVDGDPDKVVKIDDIWT
                     FARDTSSRDPNWKLSATETAH"
     gene            complement(4584..4796)
                     /locus_tag="VNY06_03445"
     CDS             complement(4584..4796)
                     /locus_tag="VNY06_03445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX13916.1"
                     /translation="MQSFGVPEENRMNIHKNARLTPVGRERIVMQVESGQTPQAAARA
                     ARICRRKWVAFTTARTPSPDPTPYCR"
     gene            5070..6368
                     /locus_tag="VNY06_03450"
     CDS             5070..6368
                     /locus_tag="VNY06_03450"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019196524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HWX13917.1"
                     /translation="MNAPASAPAKTQDLQHEKAQRCRLCGDRLEHTFVNLGMSPLCES
                     FLAADQLDAMEPYFPLHVLVCGNCFLVQLEEYVSPEHIFTEYAYFSSYSTSWVTHAKA
                     YCDMIKDRLSLGADSLAVELASNDGYLLQHFVPLGVPVLGIEPAANVAKVAVNKGIPT
                     RIDFFGTELAKNLVAEGYRADLILGNNVLAQIPDLNDFVSGMQLLLKPEGVITLEFPH
                     LQRLIEGNQFDTIYHEHFSYFSLVTIDKMAARDGLKLIDVEELTTHGGSIRVYLAHQA
                     SERKPAKAVPDMLARERDLGFLDIATYASFADQVCRTKRRLLAFLIKAKDEGKTICGY
                     GAPGKGNTLLNYCGIGVDFLDFTVDRNPYKHGRFTPGMHIPIHDVGAIDAARPDYIFI
                     LPWNLRQEIVQQMRRVGEWGGKFIVPIPEIAVIDPKELQS"
     gene            6365..6488
                     /locus_tag="VNY06_03455"
                     /pseudo
     CDS             6365..6488
                     /locus_tag="VNY06_03455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009363610.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
BASE COUNT         1384 a         1884 c         1832 g         1443 t
ORIGIN      
        1 caaaaggccc gcacctcgtg attcctttca gccgctgacc aaaacgcaag agttgagtcg
       61 cgcgtcagca agtcggtctg tgtccaaccg caggcgcgtg ctgctgttgg ctgggtactc
      121 atcttcgaag gccatggaat gtcccttttg caaattgagc tcgatcgcac cgaacatccc
      181 gtagacatca ttgagcggat tgccgcgctc aatcattggc ccttcgaccg cggcgaacag
      241 gatgaaattt ccatctcggt cgctggcggc tggacccaat atacggtcgc ctttacatgg
      301 ctcgccgaag ttgaggtggt ccacgtcgcc tgcgctttcg acctcaaaat tccagaaacg
      361 cggcgattgg aactcgcctc gctcgtttcg ctcatcaatg aagagttgtg gatcggccat
      421 ttcgacattt ggcccactga cggagtggtg atgtttaggc atgccatatt gctctcgggc
      481 ggcgccgagt tgaacagcca gcaatgccaa tccgctctcg caaggatcgt gcgcgcatgc
      541 gagcgctatt accaggcgtt tcaattcgtc gtctgggcag gcaaggcagg ccgcgaggcg
      601 ctcgctacag tcatgctgga aacccaaggc cgggcatgac atggaatcag cggaagcgga
      661 ccgcttcaac ctatcgctca tgcttgccgg tgccggcaag atgggcgggg cgatgctgcg
      721 ggcctggctc gaccagggat atgatccccg gcaaattagt gtccttgagc cacatccgtc
      781 ccaggaagtc gtgagccttg cccgcgccaa gggctttgtc ttggggtgcc tttcggagcc
      841 gccgcaagtt ctcgtgctcg cgatgaaacc gcaaagtttc gatgaagcgg ccaccggact
      901 cgagtctttt gccgatcagg gtacattgat catctctatt ctggcgggca agacctttgc
      961 caatatcgcc agcagactgc ctcgggcgag cgcgatcatc cgggcgatgc caaatctgcc
     1021 agcggcgatt gggcggggta tcaccgcgct tgccgcaaat ccggcggtga cgccggtcca
     1081 gcgttcggca gccgaagctt tgctcggtgc gacggggcgc gttgaatggc ttgccgggga
     1141 acatttgata gacgcggcgg cggccgtgtc tggcagtgga ccggcttatg tattttatct
     1201 cgctgaagtc ttggcagagg ctggcgaggc tctgggcttg cccgccgata tatcagccag
     1261 gctcgcacgc ggcacgatcg aaggtgcggg agagctgcta ttccaaagtc ccggaagcac
     1321 ggtggcagaa ctgcgcgaaa atgtaacctc tcccggcggt acgaccgccg ccgccctcga
     1381 aattctcatg tctgaagagg gtcttgcgcc cgtcatccaa cgtgcgttaa gagcggcgaa
     1441 acggcgcgca gaggccttgt ccggatagct tacccttcat gcagctcacg tgctcaatcg
     1501 gctttcttac gttcgtcgcc gccatggtgt tggctacctc atgccgcgcc gccagccagg
     1561 aagatccttt tattgagctc gagacaaatg ctcagggcgt tcacacctat gtgagacgcg
     1621 acgaaattga agccctgacc gatgtcagcc ctgctcattg tgttttgttt ctcacctctg
     1681 gaacgtcatt cccacaaagc aaaaacgtca gggcgtgtca gacatgtgct gtctcgttcc
     1741 aagcagactt tggaaacaca tatatttcac ctcaaggtat cgatacgatg gcgtggacaa
     1801 gcaattcccg ttgccgaata tctctgaaaa gccgaaagtt tgtttacgcc aaagaatcat
     1861 gcaacgaagt tcataaagcc atgcctcgcg aataggacat ccttgctgtg ccgacacaaa
     1921 aggtccctct agcggcggaa gcaaaatccg gttcaaacaa gctcagcagg ttgaggcgct
     1981 ccccggcttt gatccaatcc gacgaaacag tcagtcgatt tgttccttgg aaccaggttt
     2041 tgcacggact agataaacgg gcccatgggt gcagcccagg aggtgaatat ggctgacgaa
     2101 agcagcggca aagatgaagc gcgggctgaa gctgggccgg attgcaagac aatagcggcg
     2161 attgccaagg atccgcgaaa tgcgatcatc gacgccctcc tggagctcgc cagcgagcgt
     2221 agctgggaag atataacgat ttcggacgtc gcttctcgcg cgaatgtctc gctttccgcg
     2281 tttcgcgact attttccatc gaaaggagcg gtcctggcga gtttttcgcg ccgcatcgac
     2341 catgttgtgt tggacgcgac tggcgatgac ttagcgggcg aaccggtaaa agaccgattg
     2401 ttcgacattc ttatgcgccg ccttgacgcg cttgcgcctt ataagcttgg gcttgaggcc
     2461 gtatacgaat gggcgcggcg cgatccactg gccgcctccg cgctcaatcg agtcgttgtg
     2521 aattccatgc gctttatgct tgaagcagca ggcatcgact ccgagggccc tgttggcgcg
     2581 ttgaaattgc agggacttgc gcttgcatgg gcccgcattc tgcgggtctg gtttcaagac
     2641 gatgatccag gcctggcatc cacaatggca gctcttgacc gcgaattgac gcgaggaagc
     2701 agctttgtcg ccagagccga ggatttgaat cggctcgttt cgcccttgtt ctcaattgcg
     2761 cggtccttac tggagtcacg gccgagtcgc aggcagtccc gccctggtgg ccttggagat
     2821 cgcgagaacg aacgatctcg ttaggttgcg cggctctaag gaccaggcgg tgtaaaccgt
     2881 tacctgcatg cagcgtttcc ttggcaaagg cgttaagttt tcatatactg gccgccagac
     2941 catcacggaa gcgtttcctt cgtgttcgac ttttgctcct cctgcctgga ccattcccgc
     3001 ggggtaagat tgatgagagc gggtgtctgg ggacggcctc cgtggcgccc aagccggctg
     3061 aggcttagcc tctcgctcac ccaaagccga aacgaaggtg cggcgagcgc caagcccacc
     3121 aaatccgaaa caaatccggg cagaatcaga agcactgccc caagtccggc caatgtgcca
     3181 tcaagcagct tatctttagt taggccattg cgttccgttg tgttcttaac tgccacttga
     3241 cgcaacttga gcgccgccga tacgcccata cggcgcaaca tcgtcaaacc ggctaaactc
     3301 gtcagaatac aaagaagtat ggcacccaca aaaccgattg catgaacaac ggctccgaaa
     3361 gccgcgagct cggctccaag ccatagcgcg aagtacgtta cgattagaac cggtaagcga
     3421 aggagaggcc ggtgcacaaa tctgccatca gaccgaaggc gaagcattaa ggtcaccatt
     3481 gaggctagat ttcagctttt ttcttcccac ataagatagc ataataagcg ggtaataaaa
     3541 gcgcaccaga tttcaccccg gccatcaagt accccgatgc aagaaccgtt tgacataaca
     3601 accatcattt tcgcgttact cgctgctttt gtcgtgtgga agctgcgatc ggtgcttgga
     3661 acccgcaatg gcactgaaaa accgcctagg aatcccttcg ccactagggc tccgggcggt
     3721 tttgagaacc gaagcggtga tgccacgcct caagccatcc cacttcccgg agcggccgag
     3781 accccgattt ccgctacgcc agtcaaggac gagaaatgga aaccctatgc tgagccgggg
     3841 tccagcgcgt gggcgggatt agactcggtc gccgctgccg acccaagctt tgcaattcaa
     3901 ccattcgccg ctggggccaa attggcatat gagatgataa ttctcgcatt cgccgagggg
     3961 aaccgcaagg tcttgcggaa tcttctttcc cccgacgtct acgagggctt tgcggccgcg
     4021 ctggacgagc gcgagaggcg cggcgagaag ctttcgacga cattcgtttc catcgacggg
     4081 atgacgatcg atgatgcgac gctggttggc cgcaccgcca agatcaaggt ccatttcacg
     4141 tcccagatga tcaccgcgac acgtgacgcc aacggaacgg tggtcgacgg ggaccccgat
     4201 aaagttgtga aaattgacga tatctggacg ttcgcccgag acacgagttc ccgcgatccg
     4261 aactggaagc tcagcgccac tgaaaccgca cattaattgg ccatttcgag cggccaagca
     4321 ttaccaagga cgcctcggct gtctccccga tctcagcgct gaacgcaaga agcgaccggg
     4381 gcgacgtttt tattgcccct ttaaaattcg cggacctttc cggatttgag cgcgacgatc
     4441 acctcgaggc ttttgccgtt tcctgcagtc ctgctatcgc tgccaaattg ccccccttta
     4501 cgctcggcaa cacctgcctc tgtacctcta cgcgcagggc gatgggcttt ccgggcaacc
     4561 gctgaccgcg cagggcgatg atctcaccga caataaggag ttgggtcggg cgatggagtg
     4621 cgcgcggtcg tgaacgcgac ccattttcgg cggcagattc ttgcggctcg tgcggcggcc
     4681 tgcggcgtct gcccgctctc gacctgcatg acgattcgct ctcgaccgac cggcgtcaat
     4741 cgggcattct tgtgaatgtt catccggttc tcctctggaa caccgaagct ttgcaactcc
     4801 agtctccccg atccggaccg agtggacaac ctaatgaaag ctcacatgta taccaatgac
     4861 ttttcttcga atcgtcgtct tgctctaaat ttttgatttg tcgcgcgtcc ggacgcaaaa
     4921 ccggtttcca cttttgctgg atacgctctc ggcgctgccc gccgcagcgt gcccgaagtc
     4981 agggagcatg tcgtcgacgc cgtcatgaat ttttgggtcg atttacgcga atatgataaa
     5041 cgctgctaat caatttaagg agacctccca tgaacgcgcc agcctccgcc cccgccaaga
     5101 cgcaagattt gcaacacgag aaggcgcagc ggtgccgtct ttgcggcgat cgattggagc
     5161 ataccttcgt taaccttggc atgtctcccc tatgcgaaag cttcctcgcg gccgaccaat
     5221 tggacgcgat ggagccgtat tttccgcttc atgtgcttgt ctgtggcaat tgtttccttg
     5281 tgcagctcga ggaatatgtc agcccagagc atattttcac cgaatacgcc tatttttcct
     5341 cctactcgac ttcctgggtg acacacgcca aggcctattg cgatatgatc aaggaccggc
     5401 tttcgttggg tgccgatagt ctcgccgttg agctggccag caatgacgga tatttgctgc
     5461 agcatttcgt gccccttggc gtgcctgtcc ttggcatcga gccggccgcg aacgtagcaa
     5521 aggtcgcggt caacaaaggc attccaacgc gcatcgattt cttcggcacg gagctggcga
     5581 agaatctggt ggcggaaggc taccgggcgg atctcatcct cggcaacaat gtgcttgccc
     5641 agatacccga cctcaacgat ttcgtcagcg gaatgcagct tcttttgaag ccggagggcg
     5701 tcatcacgct ggagttcccg catctgcagc ggcttatcga aggcaatcag ttcgatacca
     5761 tttaccacga gcatttttca tacttctcgc tcgtcaccat cgataagatg gccgctcggg
     5821 acggtttgaa gctgatcgac gtggaggagc tcaccaccca cggcggctcg atccgcgttt
     5881 atctcgcgca tcaagcgtcg gagcgtaagc ccgcgaaagc ggtgcccgat atgctcgccc
     5941 gcgagcgtga tcttggtttc ctcgatattg cgacctacgc ctcgttcgcc gatcaagttt
     6001 gccgcaccaa gcgtagattg ctggcgtttt tgatcaaggc caaggacgag ggcaagacca
     6061 tctgcggcta cggcgcgccg ggcaagggca atacgctact aaattattgc ggtattggtg
     6121 tcgacttcct ggacttcacc gtcgaccgta acccttacaa gcatggccgg ttcacgcccg
     6181 gtatgcatat cccgattcat gacgtcggtg ccatcgatgc ggccagaccc gattacatct
     6241 ttattctgcc gtggaacctg cggcaggaaa tcgtgcagca aatgcggcgt gtcggcgaat
     6301 ggggcggaaa attcattgtg ccgatccccg agattgccgt cattgacccg aaggagctgc
     6361 aatcatgaag gtcgtgctgt tttgtggagg tcttggcacg cgagttcgac catattccaa
     6421 ggcgattcca agaccgatga tccccgggtc atcaacccat cctctggcac gttatgcagt
     6481 attacggcta atacggtcat cggaatttta ctgactgcgt cgtcgcagga tttggagcgc
     6541 aag
//
LOCUS       DATMPU010000147         4003 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_2286946,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000147 DATMPU010000000
VERSION     DATMPU010000147.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 4003)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4003)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4003
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2286946"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            103..513
                     /locus_tag="VNY06_03460"
                     /pseudo
     CDS             103..513
                     /locus_tag="VNY06_03460"
                     /inference="COORDINATES: protein motif:HMM:NF018745.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1345 domain-containing protein"
     gene            complement(1076..2998)
                     /locus_tag="VNY06_03465"
     CDS             complement(1076..2998)
                     /locus_tag="VNY06_03465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012590598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HWX13918.1"
                     /translation="MTKAIAPYGAWMSPVSVELVTEAAIGLSALSVDGEQLYWLEARP
                     SEKGRTVLCRRRLNGEIEDLTPPPFNVGSRVHEYGGGAYRAQAGVVIFSERMDGSVWI
                     IEDGAEPRRIATPEGCRYADFELDLPRRRTFAIREDHRGRPPTDPKAAIVALPLDPGE
                     AELVLFEGPDFLSSPRLSPLGERLAFIAWDHPDMPWDQTRLCVADVTREGTLQPARIA
                     AGETQKAIVEPKWSPDGTLHFCSDRSGWWNLYALRTKKVEALAPVEAEIGGPHWVFRQ
                     RFYDFLPNGSIIAAMVCDGMRSSVLIADGKIAPLAIGQVQECPCAFGRGLAFIATPPS
                     SPSAASVLPTIEGSAPTTIRAAAPAAISQESLSVGNPIDFSTPGGTAHAFWYPPKNRD
                     YEGPPGELPPLIVLSHGGPTSMASNSFSLSIQWWTSRGFGVVDVNYGGSTGYGRIYRE
                     RLYGQWGIVDVEDCAAAAECLIDRGLVDGNHVIIRGGSAGGFTTLAALTSRNLFRAGA
                     SYYGVGDLILLAKDTHKFESRYLDKLIGPLPEAEALYRERSPVNHLDRLSCPVIFFQG
                     QEDKTVPPNQAETMVEAMKGRGLPVAYYSFADEGHGFRKAETLRRALELELDFYGRVF
                     GFTAPNLRERVIIANM"
     gene            complement(3113..3703)
                     /locus_tag="VNY06_03470"
     CDS             complement(3113..3703)
                     /locus_tag="VNY06_03470"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HWX13919.1"
                     /translation="MLAAARAAGVRRFVAQSFAGWLYAREGSFVKTEKDPLDPNPPPT
                     FRHTLEAIRYLESVVMSEALLEGIALRYGAFYGPGTAIGEHAWMVNDIRNRRVPIIGE
                     GGGIWSFIHIDDAARFTLAALTRGAPGIYNIVDDDPAPVSQWLPALAEALDAKPPRWV
                     PAWLARILIGNAVVVMTEARGASNAKAKAIFGSDLT"
     gene            complement(3883..3987)
                     /locus_tag="VNY06_03475"
                     /pseudo
     CDS             complement(3883..3987)
                     /locus_tag="VNY06_03475"
                     /inference="COORDINATES: protein motif:HMM:NF017206.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
BASE COUNT          887 a         1197 c         1128 g          791 t
ORIGIN      
        1 tcatgatcgc gcgggcgtcc ccgcaatcga tccggcggcg ggctaactga tgaggggaaa
       61 tccttcattc tagtcttcac gactctagca gcgcttgcgg cgatcgcggc gatcacggtt
      121 caactcgccg ccgtggccgc cacggaggat ttaccgaaga cacttaaggg cctgcatatc
      181 ggacttgcgg cggcaacgat catcagcgcc tggttcttca tacatctcac ttacgcactc
      241 cattacgcgc atgaatattt cgacgaatat gtcgcctaat cgggcaggcc ggcaaccgag
      301 cggggcggcc tgatttttcc tggaaacgag gaacccgatt attatgactt tctgtatttt
      361 tcctatgtaa ttggtgttgc ctgtcagact gccgacgttg agctttcgtc tcatgcgatg
      421 cgcaggttgg ccctcgtcca ttgcatttta gcgttgttct tcaacagcgc gctgctggcg
      481 cttactatca atatagccgc cggcttaatt tgaggcaggt caaggccgca accgcgatct
      541 tgtcgcttca cgagattcaa tagccagctg tggccagagt ggggggaacc ttcaagcgag
      601 actcagtgtt acctacccct gcggttctgc aggaggagat gtcacgacgg ctggccatcc
      661 accaatccac gcggctcaag cctcagcgca tgcagcggcc ttagccagga gttccacatg
      721 agaaaattat tttttgtgcc cgtcatcggc agcctagctg ccctaggctt catcggccaa
      781 gcagaggcgc ggcccggagg ctgcataaag gatgcgatcg ttggcggaat cgctggccac
      841 ttcgttgggc atggcggcct cggagctgct gccggctgcg catatggaat gcacgagcgg
      901 cataagtata atcgccaaca agaacgccgg tcgagttcgc aacagcgtga catatacgag
      961 ccgcgccgat aaaggaacgc gcgagcgaaa agcgcgccag cgtgggggcc agacaagcgc
     1021 ggccgccgac tcgtcgcttc attgtgatgc agggcttcat tatgctgcag gccgctcaca
     1081 tattggcgat aatgacgcgt tcgcgcaaat ttggcgcagt gaaaccaaac acgcggccgt
     1141 aaaagtcgag ctccaattca agcgcgcggc gaagagtctc cgccttgcga aagccatggc
     1201 cttcgtccgc aaatgagtaa taggcaacag gtaaaccccg ccccttcatc gcttcgacca
     1261 ttgtttccgc ctgattgggc ggcacagtct tgtcttcctg gccttgaaaa aagatcacgg
     1321 ggcaggaaag tctatcgaga tggttgacgg gcgaccgctc acgataaagc gcctcggcct
     1381 caggcaaggg cccgatgagc ttatcgagat agcgggactc gaatttatgc gtatccttgg
     1441 ccagcagtat cagatcgccg acgccataat agctcgcccc tgccctgaaa aggttgcgcg
     1501 acgtcagcgc ggcaagcgtt gtgaagcctc ccgcgctacc cccgcgaatg ataacgtggt
     1561 tgccatccac aagcccgcga tcaatgaggc actcagcggc cgccgcgcaa tcttcaacgt
     1621 cgacgatacc ccattggcca taaagccgct cgcggtagat gcggccataa ccggtcgaac
     1681 ctccataatt tacgtcaacg acgccaaaac cccggctcgt ccaccattgg atgctcaaag
     1741 aaaaactatt cgacgccatg ctcgtcggcc ccccgtggga caagacgata agcgggggca
     1801 actctccggg tgggccttca tagtcgcggt ttttcggcgg ataccaaaat gcatgcgccg
     1861 tgccacctgg ggtggagaaa tcaatcggat ttccaactga gagactctct tgcgagattg
     1921 cagctggggc tgcggcgcgg atcgtggttg gtgcgcttcc ctctatcgtt ggcaacaccg
     1981 acgcagcgga cggcgaagag ggaggcgttg caataaaagc caaaccacgc ccgaatgcgc
     2041 acgggcactc ctgcacctga ccaatagcga gcggcgcgat ctttccgtct gcaatcagta
     2101 ccgaagatcg cattccgtca caaaccatcg cggcaatgat actgccattg ggcagaaaat
     2161 cgtaaaagcg ctgacgaaag acccaatgag gcccgccgat ctcggcctcg actggggcca
     2221 gagcttcaac tttctttgtt cgcaaagcat aaagattcca ccagccgctc cggtcggagc
     2281 aaaaatgcag cgtgccatca ggagaccatt tgggttcgac gattgctttt tgcgtttccc
     2341 ccgccgcaat ccgcgcaggt tgaagcgtgc cctctcgcgt tacatccgct acgcaaagcc
     2401 ttgtttgatc ccaaggcata tcgggatgat cccaggcgat aaacgcaagc ctttcgccca
     2461 aaggcgaaag ccgcggcgaa cttaaaaaat ccgggccctc gaacaaaacc aattcggctt
     2521 cgccgggatc aagcggcaac gcaacgattg cagcttttgg atcggtgggc gggcgcccgc
     2581 gatgatcttc ccggatcgcg aaagtacgcc ggcgcggaag gtcaagttca aagtcggcat
     2641 aacggcaacc ctccggcgtt gcgatccggc gcggttcggc gccatcttca atgatccaca
     2701 cgctgccgtc catgcgctcg ctgaaaatga cgacgccggc ctgcgcacga taggcgccgc
     2761 cgccatattc atgcacgcgg ctgccgacat tgaaaggcgg cggcgtgagg tcttcaatct
     2821 cgccgttgag gcggcggcgg cataaaaccg tgcgtccctt ctcgcttggc cgggcttcga
     2881 gccaataaag ttgttcgcca tcaacagaga gagcgctaag gccgattgcc gcttcggtca
     2941 cgagttcgac actgaccggc gacatccagg cgccgtaggg ggcgatagct ttggtcatac
     3001 cccacttatc ggctatgtaa agcggcaagc caagcccatc gacgatggta ggagacttag
     3061 cgcagctgcg tgtgcgcctg cgtttgcgtc aaaatccttc ccgccagctt ggtcatgtca
     3121 aatccgatcc gaagatggct tttgccttgg cgttcgaagc gccacgcgct tcggtcatga
     3181 cgacaacggc attgccaatc aaaatgcgag cgagccacgc tgggacccag cgtggcggct
     3241 tggcatcaag cgcttcggcc agggcaggca gccactgcga taccggagca ggatcgtcat
     3301 cgacgatatt gtagataccc ggcgcgcccc gcgttaaggc ggctagggta aagcgggcag
     3361 catcgtcaat gtgaataaag gaccaaatac cgccgccttc accaattatc ggcacgcgcc
     3421 ggttgcgtat gtcatttacc atccaggcat gctcacctat agccgtgccc ggaccgtaaa
     3481 aagctccata gcgcaaagca atcccttcta agagcgcttc ggacatgacc acagattcga
     3541 gatatcggat cgcctcgagg gtatggcgaa acgttggcgg cggattggga tcgagcgggt
     3601 ccttctctgt tttgacaaag cttccctccc gcgcgtagag ccaacctgca aagctttgcg
     3661 cgacgaaacg gcgcacccct gcggcacgcg cggccgccag caaattatcc gtgccctctt
     3721 ttcgcagccg gttggtcgcg gcgaatcccc catcgatttt cgccggtcga ccttcccgat
     3781 agaagtgagc tcgtgaataa tagtttgcgg ccttgtttgc cagggatcaa gtgcatcggc
     3841 gacgatcgcc tcggcaccca tcctctgcaa aagactcaac ttggcaggag agcgcgtcaa
     3901 accggcgacg ctgtgccccg ccgcaatcag taaaggaaca aggcgccggc cgagggctcc
     3961 agttgctccg gcaacgagaa ctctcattga agtgaaacaa ccc
//
LOCUS       DATMPU010000148         3468 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_2597403,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000148 DATMPU010000000
VERSION     DATMPU010000148.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 3468)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3468)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3468
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2597403"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..111)
                     /locus_tag="VNY06_03480"
     CDS             complement(<1..111)
                     /locus_tag="VNY06_03480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011500848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HWX13920.1"
                     /translation="MANGQGNGEAAETETAPSINVMVQYTKDFSFENPNAP"
     gene            407..2101
                     /locus_tag="VNY06_03485"
     CDS             407..2101
                     /locus_tag="VNY06_03485"
                     /inference="COORDINATES: protein motif:HMM:NF014622.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HWX13921.1"
                     /translation="MAIRRKVILLLEVLLAMPMEPPRFYLITPPISNTAPYPELSGEA
                     MAGCGVTCLLVRMAAYGDAENEIIFHRLAAPLQERGIACLVADDPQFCVRVHADGVHV
                     NGEGPQLEQALRSLKPGLIVGAGGLRTRHAAMVAGEAGVDYVMFGEGAEQQATLVKQI
                     AWWAEIFAVPCVGYAHDLDSIGDLVCAGADFIALGGAAFSDPRGPQAALHVAAALVTR
                     APETVMAGPRNLRKCFRVSLMAAWAILACACLFLPARAGAGEPRPAENPTGEQGETAA
                     ESLPVPARLGGAPDIAFGAYQRGYYLTAMREAVKRVNADPNDGPAMTLIGELYAQGLG
                     VRRDPTEAARWYKLAADRSDPQATFALAMAKLNGEGVPKDRSGAAQLLEKAAVQDHPG
                     ALYNLGVLAIENDGVTKNFEKAAELFKRAADLGNSDGGYALGLLYKNGSGVEKSEQEA
                     ASWIGRAAKDGNVPAEVEYAIMLFNGAGIEKNETAAAKLFLKAAVSNNPVAQNRAARI
                     LAAGRGLPKDMVEAMKWHLLAKAAGIKDSWLDGELAKLSPQEKIAVETNLRHYMGN"
     gene            complement(2467..>3468)
                     /locus_tag="VNY06_03490"
     CDS             complement(2467..>3468)
                     /locus_tag="VNY06_03490"
                     /inference="COORDINATES: protein motif:HMM:NF012425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase family protein"
                     /protein_id="HWX13922.1"
                     /translation="ENEPQLALQIAEAIATKIQQGPRPAVRDAHPKAHGCVAAEFRVE
                     DDLPQYLAQGVFTPGRTYPAYIRFSNGNPDPNRDDAKGDARGMAIKLLEVPGEKILPD
                     ERDAQTQDFIMINHPVFFIDDPAHYLGLIQRTGSSNPLALVGQLLALGLKGALIAKAV
                     TSSKIASPLETRYWSIVPYRLGDPPHKQAIKFSARPRGPGATSLPDDPTPNFLRETMI
                     RQLEAGKAQFDFEVQPREPSMSVEESTVEWKEEEAPFIKVATITIPRQTFATTERDRF
                     GENLSFTPWHALPQHRPLGSVNRIRRVVYQTISKLRHELNGTAPREPSGFDADMGRA"
BASE COUNT          693 a          963 c         1083 g          729 t
ORIGIN      
        1 cggcgcattc gggttctcga aagaaaaatc cttggtgtat tgcaccatca cattgatgga
       61 gggtgccgtc tcagtctccg cggcttcgcc gttgccttgt ccgttagcca ttgcatcgat
      121 ctcccggggc tgcgggtagc acggatgtag cttcccgaca agcggaccgg atgaggagaa
      181 ttgcggcttt gcgcaagagt actcgggcgg gttctcgtgc ggattggctc gccctatgct
      241 gaggctgcgg atcaggcaac ggtgggccgg ccgatcaaaa ttcagtccca aagaggagcg
      301 aaaatccact atggtcgcac tagtctagcg gtctacggcg tgtcgggcca aaggtgagaa
      361 ccggttttgc gcctggacac gcgacaagct aagcatctag cgcaagatgg cgattcggag
      421 aaaagtcatc ttgcttttgg aggtactgct cgcgatgccg atggagccgc ctcgctttta
      481 tcttatcacc ccgccgatct ccaacacggc tccttatccg gagctctctg gcgaggcgat
      541 ggcggggtgc ggcgtgacgt gcctgcttgt acggatggca gcgtatggcg atgccgagaa
      601 tgaaataatc ttccatcggc tggccgcgcc gctgcaagag cgcggaattg cgtgccttgt
      661 ggcggacgat ccgcaatttt gtgtccgtgt tcatgccgat ggcgttcatg tgaacgggga
      721 gggaccgcag ctggagcagg ctctgcggtc gctaaaaccc gggcttattg tcggtgcggg
      781 agggctacga acgcggcatg ccgcgatggt tgctggcgaa gcgggcgtcg actatgtcat
      841 gttcggcgag ggcgcagagc agcaagcaac tctcgtcaag cagatcgctt ggtgggcgga
      901 aatttttgct gttccttgcg tcggctacgc tcacgacctt gactcgatcg gtgaccttgt
      961 ttgcgccggg gctgatttca ttgcccttgg cggcgcagcc ttttccgatc cacgcgggcc
     1021 ccaagcggcg ttgcacgtgg ccgctgcgct tgtcacgcga gcgccggaaa cagtgatggc
     1081 tggtccccgt aacttgcgaa agtgcttccg cgtttcactc atggcggcat gggcgatatt
     1141 ggcatgtgcc tgtcttttcc tcccagcgcg agcgggagct ggcgagccaa gaccggctga
     1201 aaacccgacg ggcgagcagg gcgaaactgc ggcggaatct ctacccgttc cagcccggct
     1261 cggcggtgcg cctgatatcg cctttggcgc ctatcagcgc ggttattact taactgcgat
     1321 gcgcgaagcc gtgaagagag taaatgccga tcccaacgac ggacccgcga tgaccttgat
     1381 tggcgagctt tatgcgcagg ggcttggagt ccggcgcgat ccgaccgaag cagcccgctg
     1441 gtacaaactc gcggcggatc gatctgatcc gcaagcgaca ttcgccctcg ctatggccaa
     1501 attgaacggc gaaggcgttc cgaaggatcg ctctggggct gcgcagcttt tagaaaaggc
     1561 agcggtgcaa gaccatcctg gggcgctcta taatcttggg gtgctcgcca tcgaaaatga
     1621 tggcgtcacg aagaattttg aaaaggcggc agagcttttc aagagagccg cggacctcgg
     1681 caactcagac ggtggttacg ccctgggtct cctctacaaa aacggcagcg gcgtcgaaaa
     1741 aagcgagcaa gaagccgcga gctggattgg ccgtgcagcg aaagacggga acgttcctgc
     1801 cgaagtcgaa tatgcaatca tgctgttcaa cggcgccggc attgagaaaa acgagacggc
     1861 ggcggcgaag ctttttctca aagcggcggt gagcaataat ccggtcgcgc aaaaccgcgc
     1921 ggcgcgcatt ctcgcggctg ggcgcggctt gccaaaggac atggtcgaag cgatgaaatg
     1981 gcatctcctc gcgaaagccg cgggcatcaa ggactcttgg ctcgatggcg agctggccaa
     2041 gctatcgccg caggagaaaa tagccgtcga gacgaacttg cggcattata tgggaaatta
     2101 gctgtttagt cccggcattc gtcgcggtcc atatgggtcg tctgtagaag gttgtcgtcg
     2161 ccctactcca tcacctgccg ttccgagcaa atccggtaaa cgacgtgcag cagcacggcg
     2221 ctcagcaatt gctcggcggg atcgacgggc gaccctcgct tgcataaagc tttccgaaac
     2281 tatggctgag ttttttcagc tcgatctgcc gtagaggatg cctcagcggg acccgtgcat
     2341 ctggcgaaat ggcggaaaac aattcacctt ggtcccgaaa tcgcccgcgc atggcgcctt
     2401 aaccgatttg tgaccaaagt gaatcatcaa aaagcagcca aaaccagggt tactgccaaa
     2461 tccggctcac gcacggccca tgtcagcatc aaacccgctt ggctcgcgcg gcgctgttcc
     2521 gttcaattca tgacgcaact tggaaatggt ctggtagacg acccgccgaa tgcggttcac
     2581 tgagccgagc gggcgatgct gcggcagcgc gtgccagggc gtgaacgaaa gattctcgcc
     2641 gaaccgatcg cgctccgtgg tcgcaaatgt ttgccgcgga atagtgatag tggccacctt
     2701 gatgaatggt gcttcttcct ctttccactc caccgtcgat tcctcgacgc tcatgctcgg
     2761 ctcgcgcggc tgaacctcga agtcaaattg cgctttccct gcttcaagtt gtctgatcat
     2821 cgtttcgcgc agaaagttcg gtgttggatc atcgggaagg gacgtggccc ctggaccacg
     2881 aggccgcgcg gagaacttga ttgcttgctt gtgtggcggg tcgccaagcc tgtaggggac
     2941 tatcgaccag tagcgcgttt cgagcggact tgcgattttc gacgaagtca ccgctttcgc
     3001 aataagcgca ccctttaggc cgagggcaag gagttggcca accagtgcga gggggttgga
     3061 gctgccggtc cgctggatca gcccaagata gtgcgcagga tcgtctatga agaacacggg
     3121 atggttgatc atgatgaagt cttgcgtctg agcgtcgcgc tcgtcgggca agatcttttc
     3181 gcccggcacc tcgagaagct tgatggccat gcctctcgcg tctcccttgg cgtcatcccg
     3241 gttggggtcg gggttaccgt tcgaaaaacg gatataggct ggataggtcc ttcccggcgt
     3301 gaagacgccc tgcgctagat attgcggcag atcatcctcg acacgaaact cggccgcaac
     3361 acaaccatgc gcctttggat gggcatcacg aaccgctggg cgcggcccct gctggatttt
     3421 tgtggcgatg gcttcggcga tctgcagagc gagttgcggc tcgttttc
//
LOCUS       DATMPU010000149         2872 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_1061425,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000149 DATMPU010000000
VERSION     DATMPU010000149.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 2872)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2872)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2872
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1061425"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(105..1937)
                     /locus_tag="VNY06_03495"
                     /pseudo
     CDS             complement(105..1937)
                     /locus_tag="VNY06_03495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003610513.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase"
     gene            complement(1946..2095)
                     /locus_tag="VNY06_03500"
     CDS             complement(1946..2095)
                     /locus_tag="VNY06_03500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003526460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5989 family protein"
                     /protein_id="HWX13923.1"
                     /translation="MSVVTELFEFLLTRKKFWLIPLLALFLIFGGLLVLSQGSVVAPF
                     IYTLF"
     gene            complement(2106..2528)
                     /locus_tag="VNY06_03505"
     CDS             complement(2106..2528)
                     /locus_tag="VNY06_03505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003610510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SxtJ family membrane protein"
                     /protein_id="HWX13924.1"
                     /translation="MQTHETVSSFRSVAIGSDRKFGAAFGLAFSVLGIWPVVHHHSPR
                     WWLITIAGLFFAAAVLFPRSLAPLNRAWFKLGLVLNRIVSPIVMGGLFFCALVPIGWY
                     LRKRGKDLLSLKLDGNATTYWIERNPPGPAPNSLTKQF"
     gene            complement(2691..>2872)
                     /locus_tag="VNY06_03510"
     CDS             complement(2691..>2872)
                     /locus_tag="VNY06_03510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX13925.1"
                     /translation="KKHLLLPWKSKRGDLHATDHPYLATISRPSDATTLAVRPRRRGP
                     PAPQASLVFRDKHFL"
BASE COUNT          698 a          778 c          826 g          570 t
ORIGIN      
        1 cgccagtcgc aaatcgagcc cgggcgatca tgcttaagga tttcatcggc cggcagatgc
       61 ccgggatatt gcgcgcggca ccacagtaag cgccccaaag accgttagtc gagcgcgaag
      121 gcgctcttgt agtcgcgggc aagttctggt ttttgctttt ctttgcgaaa atgcaatttc
      181 cgaccgcgag gaactcgatt tcactgccca tgaaacagga aaacgcgtcc tctggcgtgc
      241 aaacgatggg ctcgccgcgc acgttgaaac tggtgttcac gaggacggga cagccggtct
      301 gctcgaaacg gctgatcagc gcgtggtagc gcgggttcgt ctccttgtgg acggtttgaa
      361 tgcgggctga ataatcgaca tgggtgacgg cgggaatgtc cgagcgaagc acgttgagct
      421 tgtcgatgcc gaacagcgct cgtgccgaca tcttgcggca gcgcgccggt ttcacatttg
      481 cgacgagcaa catatagggc gaatccgcgt cgagctcgaa aaaatccgcg acgcgctcgc
      541 gcaataccga tgacgcgaaa ggacggaagc tttcgcgata ttttactttg agattgagtg
      601 ccttttgcat tgttggcgag cgcggatcgc cgaggatcga acggttgcca agtgcgcgcg
      661 gcccaaattc catccgaccc tgatgccatc cgaccgcctg gccgttcaca agtgcggccg
      721 cggtgtcatt tataagctgc tcgtctgggc aagcgttaat agactgcgcc tatagctctg
      781 agacgctgct cgatatcggc ctgcctatac gagggaccga gaaacgcccc ctgcatggcg
      841 tcaggctgat ctatgatccg ctcgccgcca gtcgtgcggc gactatcata ataggcggca
      901 agcgcggcac caagcgctcc acccgcgtct ccggcggccg gctgaacgaa gatttgattg
      961 aaatggctat cgcgaagcac tcttccgttc gccacgcaat ttagcgcaac acctcccgca
     1021 agacagagat tctcaagctt cgtttgcgct gaaagcgatc gcgtcagacg caagacgact
     1081 tcctcggtca ccgcctgaac cgaagccgcg agatccatat gacgctgttc gagagggtca
     1141 gagggcttac gtggcgggcc gccaaagatt ccgcagaagc gatcatcgac cattgtgaga
     1201 ccggtacagt aagaaaaata ttcgagattg agccgaaagg agccgtctgg cttcacgtca
     1261 ataagatggt cgaaaattgc ctttgcgtat ttcggttcgc cataaggggc aaggcccatg
     1321 actttatatt cgccggaatt gaccttgaac cccgtataat aggtgaaagc cgaataaagc
     1381 agcccgagcg aatgcggaaa atgaatctcc cggataattt gcagctcggg gccttggccg
     1441 agcgcggctg aggccgtcgc ccattcgccg accccgtcca tggtcaagac aagcgccgat
     1501 tggaacggcg aaggataata ggcggaagca gcatgcgaat aatgatgctc gcaaaaacgc
     1561 agcttatctt tgtctccgag ccccggagta attgcgtcga gctcacgcaa aaggagatca
     1621 cgctgaaaga gcttctcgct gatccaaagc ggcatggcct tgcggaagga agcaaatccg
     1681 cgcggcgcga aagcaagata ggtttcaatg agccgttcga attttaaaaa aggcttctca
     1741 taaaaaacga cgcaatcaat gctctttgcg tcagccttgg cttccgtgag gcaataggta
     1801 atcgcgttgg ccggaaagcc ggggtcatgc tttcggcggg taaaacgctc ttcctgagcc
     1861 gcggcgacaa tctcaccatc gcggacaacc gcggcggcgc tgtcatgata gaaagccgaa
     1921 atgccgagaa cgagcatcca gcggatcaga aaagcgtata gatgaagggt gcgaccaccg
     1981 agccttgact cagcaccaac aaaccaccga aaatcagaaa tagcgccagc agaggtatga
     2041 gccaaaattt cttgcgcgtg agcaagaatt cgaacagctc ggttacaacc gacatcgttt
     2101 gatccttaaa actgttttgt cagactgttg ggtgcgggac ccggaggatt gcgctcgatc
     2161 caataggttg ttgcgttccc gtcgagcttg aggctcagca ggtccttgcc gcgcttacga
     2221 agataccagc cgatggggac gagggcgcaa aaaaacaacc ctcccatgac gatcgggctg
     2281 acaattctgt tcaaaacgag gcccaatttg aaccacgcgc ggttgagcgg ggccaacgaa
     2341 cgaggaaaaa gaactgctgc cgcgaaaaac aagccagcga tcgtaatcaa ccaccaacgc
     2401 ggcgaatggt gatgaacgac cggccaaata cccaaaacgc tgaatgcgag gccgaaagcg
     2461 gcgccgaatt tccggtctga gccgatggcg acgctgcgga agctcgaaac tgtttcatgc
     2521 gtctgcatca ggcgccgatc cctctttctc ggcccttgga ccctgtcaag gcaatccctc
     2581 ccttcagagt ctcaatttcc cttcccgaag gcatagtacc gattcgctca aggcacgcaa
     2641 gagagtcaca gatgcggtat tgtgatgcgc ccttatgctt cggcattgcg tcagagaaaa
     2701 tgcttgtcac gaaacaccaa agaagcttga ggtgctggcg ggcctcgacg acgggggcgc
     2761 actgccaaag tggttgcgtc gcttggccgc gagatggtgg caaggtaagg atgatcggtt
     2821 gcatggagat cgccccgttt gcttttccag ggaagcagga gatgtttctt tg
//
LOCUS       DATMPU010000150         6219 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_3221129,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000150 DATMPU010000000
VERSION     DATMPU010000150.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 6219)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6219)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6219
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3221129"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            112..1470
                     /gene="miaB"
                     /locus_tag="VNY06_03515"
     CDS             112..1470
                     /gene="miaB"
                     /locus_tag="VNY06_03515"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006935416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HWX13926.1"
                     /translation="MYIKSYGCQMNVYDAQRIADILVPDGYAETASIEEADLVILNTC
                     HIREHASEKVFSELGKLRVLKAARASNGKETKIVVAGCVAQAEGEEIFRRQQAVDVVI
                     GPQSYHRLPKILRRPQTKESVADTEFPAQSKFDFLVPPSAQSIRARGVSAFVTVQEGC
                     DKFCTFCVVPYTRGVEASRSVQSIVAEVEALGLAGVREVTLLGQNVNAYHGATHDAGS
                     ATLAKLCARLGEVPGISRIRYTTSHPGDMDSSLIAAHRDLPSLMPFLHLPVQSGSDKI
                     LAAMNRKHARKDYIALIDEIRSARPDIALSSDFIVGFPGESEDDFEATLGLVETIGFA
                     NAFSFKYSARPGTPAAESSHQISEAVKAERLSRLQILLDEQRRAFNAKTVGRTIDVLV
                     EKPGRHAGQIAGKSPYLQPVQMEGSLDYIGEIVRAEIVSAGPHSLFARLLPGHAASEA
                     LR"
     gene            1551..2522
                     /locus_tag="VNY06_03520"
     CDS             1551..2522
                     /locus_tag="VNY06_03520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003602886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HWX13927.1"
                     /translation="MAFDDNRHASLVFGQYDQNLAKLERRLGVVANANGNHVTIKGPA
                     EACEHARLVLENLYTRVELGQSVDLGDVDGAIEEGALQGTLFPKEQETRRGVFEQIGT
                     RRSGKVHARNAAQDLYLRSMKSHELVFAEGPAGTGKTWLAVGFSVLLLEQGAVERLIL
                     SRPALEAGERLGFLPGDMRDKVDPYLRPIYDALADFMDARMLERGMQTGMIEVAPLAF
                     MRGRTLTNACILLDEAQNATSMQMKMFLTRLGEGSRMIVTGDPSQTDLPPGQKSGLSE
                     AIKLLSGLEGIGHVVFREADVVRHDLVRRIVGAYETAAALKAKTERQ"
     gene            2519..3025
                     /gene="ybeY"
                     /locus_tag="VNY06_03525"
     CDS             2519..3025
                     /gene="ybeY"
                     /locus_tag="VNY06_03525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016919023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HWX13928.1"
                     /translation="MNPVIDIAVEAEEWTQLEDHPRLVEAAILAAVGESGISLGPNAE
                     VSVLLCDDAYIQKLNQKWRGIDKPTNVLSFPAPHDVGSSGLLGDIVIAFETAAKEAAA
                     AQIPLHEHVAHLLVHGFLHLAGHDHIKTLEAEAMEMIERAALGRLGISDPYSASLVEE
                     AACANERA"
     gene            3117..4070
                     /locus_tag="VNY06_03530"
     CDS             3117..4070
                     /locus_tag="VNY06_03530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012254488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HWX13929.1"
                     /translation="MSTRSDIEVVLADTSIEDDISSHERALLKNVLALHEVHVGDVMI
                     PRADIVAVSLQAKLADVLEVFRSAGHSRLPVYSETLDEPRGMVHIRDFVAYLTAAANE
                     PHGKVCADGESPAEPQSDSVRAGGFLRRDRDMTLSEANILRPVLFVPASMPALDLLVK
                     MQATRTHMALVIDEYGGTEGLASIEDIMEMIVGDIEDEHDLDEGPKIETRPGGHFVAD
                     ARAGLEEVSSAIGADLTAISDAEEVDTLGGLIITLAGHVPARGEIITGDGIEFEIVDA
                     EPRRVKRVQIRIAGTSSAAADIEKRLPPEEPARQASLPPRR"
     gene            4502..6142
                     /gene="lnt"
                     /locus_tag="VNY06_03535"
     CDS             4502..6142
                     /gene="lnt"
                     /locus_tag="VNY06_03535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020177204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HWX13930.1"
                     /translation="MLAIATEVTLASGWCRSIIAFVTGATGAFAMAPFDFFPALMIPM
                     TAAIWLIDGCAEIRQKSRFPWMSLGLPLAAWRAFSAGWWWGFGYFTAGLWWLGAAFFV
                     DAEEFAWALPLGVAGLPAVLAVFSGLGFMLARLVWLPGAGRLFAFAAGLSFAEWLRGH
                     LFTGFPWNVFGMALGGNLVTAQLASVVGLYGLTIVAILIFSAPAFFGDKPNARWIVCR
                     VPLPVAAAVLAFAGICGFGALRLSEPPPKMISGVNLRIMQPNVAQDEKFRPENGAEIL
                     ARYLALSVREPLGLDGVSLLIWPESAFPFILSRSPWALSEIGEALSKNTLLVTGAARA
                     DGGARTVGRPHYLNAVQVIASGGHILDSYDKVHLVPFGEYLPFQSIFDRLGLRQFVHI
                     PGGFEAGAGPRLLVVPGLPSVVPLICYEAIFPSEVVPRGAARERPGALLNVTNDGWFG
                     ETPGPYQHFAQARLRAIEEGLPLIRAANSGISAIVDPYGRILAKLPLGVEGVLDGGLP
                     QSIAAPPFAGFPFTIVSLFWVLVLSFALAVRFRELNAD"
BASE COUNT         1236 a         1786 c         1788 g         1409 t
ORIGIN      
        1 tggccgaggc ctcgcagacc aagggagata atgtcaaggt tagataaaat tcttgctgcc
       61 caaagaacaa cggaagcagc cgagcaagcg ccgccaaaca cggctctccg tatgtatatc
      121 aagtcttatg gctgccagat gaatgtctat gacgcgcaaa ggattgctga catcctagtg
      181 ccggatggct atgccgagac tgcgtcgata gaggaagctg acctcgttat cttgaacacc
      241 tgtcatattc gtgagcatgc cagcgaaaaa gtgttctcgg aattgggtaa gctgcgcgtt
      301 ctcaaggccg cgcgcgcttc gaacggaaaa gagacaaaaa tcgttgttgc aggctgcgtc
      361 gcgcaggccg agggcgaaga gattttccgc cgccagcaag ccgtggatgt cgtcattggg
      421 ccgcagagct accatcgtct gcccaaaatt cttcgccgac ctcagacgaa ggagagtgtt
      481 gcagacaccg aatttccggc ccaatccaaa ttcgactttc tcgttcctcc gtcagcccag
      541 tcgattcgag cgcgcggggt cagtgctttc gtgacggtgc aagaaggttg cgataagttt
      601 tgtaccttct gcgtggtgcc ctatacgcgt ggcgtggaag cctcccgctc ggtccaaagc
      661 attgtagccg aagttgaagc tcttggctta gccggcgtac gcgaggtgac tttgcttggc
      721 caaaacgtca atgcctatca tggcgcgaca catgatgccg gttctgccac gctcgccaag
      781 ctttgcgcgc gcctcgggga agtgccgggt atttcacgca ttcgttacac cacaagtcat
      841 cctggagaca tggactcgag ccttatagca gctcatcgcg atttgccgtc cctcatgccc
      901 ttcctccatc tgcctgtcca atcagggtct gacaagatcc tcgccgccat gaaccgcaaa
      961 cacgcgcgca aggactatat tgccctcatt gacgagattc gcagcgcgcg gcctgacatc
     1021 gccctttctt ccgactttat tgtgggattc cctggcgaaa gcgaggacga cttcgaagca
     1081 accttgggtc tcgttgaaac aattggcttt gcgaacgcct tctcgttcaa atattcggca
     1141 aggccgggca cccccgcggc cgaatcttcg catcagattt ccgaggccgt aaaggcagaa
     1201 cggctctccc gacttcaaat tcttcttgac gagcagcggc gtgccttcaa cgccaaaact
     1261 gtcggacgca cgatcgatgt gctggttgaa aagccgggcc gccacgcggg ccagatcgca
     1321 ggcaagtctc cctatctcca gccggttcag atggagggca gccttgacta cataggtgag
     1381 atcgttcgcg ccgagattgt ctccgcaggc cctcactctt tgtttgcacg gctattaccc
     1441 ggccatgctg ctagcgaggc ccttcgttga aaccgaacga tcgtgcagta cctttcacgc
     1501 ggaccgaatt caagcggccc tcgggtgaaa acggcgcggc agaggtcacc ttggcgtttg
     1561 atgacaatcg ccatgcatcg ctcgtgttcg gccaatatga tcagaacctc gccaaacttg
     1621 aacgcaggct cggcgtggtc gccaacgcga atggcaatca cgtgacgatc aagggcccgg
     1681 ctgaggcgtg cgagcatgcg cggctggtac ttgagaacct ttacacgcga gtagagcttg
     1741 gacaatccgt cgatctcggc gacgtcgatg gcgcaatcga ggagggcgca ctgcagggca
     1801 cgcttttccc gaaagagcag gagactcgac gaggcgtttt cgagcaaatt ggcacgcgcc
     1861 ggagcggcaa ggtccacgcc cgcaacgcgg ctcaggacct gtatttgcgg tctatgaaaa
     1921 gccatgaact ggttttcgcc gaggggcccg ccggcactgg aaagacgtgg ctcgccgttg
     1981 gcttttccgt tctcctgctt gaacaaggcg ctgtcgagcg cctcatcctt tcgcgcccag
     2041 cactcgaagc aggcgagaga cttggctttt tgcctggtga tatgcgcgat aaggtagatc
     2101 cctacttgcg cccgatctat gatgccctcg ccgatttcat ggatgcgcgt atgctcgagc
     2161 gtggaatgca gaccgggatg atcgaagtcg cgccacttgc gtttatgcgc ggtagaacat
     2221 tgaccaacgc ctgcatactt ctcgacgagg cccaaaacgc gacctctatg caaatgaaaa
     2281 tgtttctcac ccgccttggc gaggggtcgc gcatgatcgt cacgggagat ccttcgcaga
     2341 ccgatctccc tcccggccag aagtccggac ttagcgaagc gatcaaactt ctctcgggtc
     2401 tggagggaat tggccacgtc gtctttcggg aagccgatgt cgttcgccac gatctcgtgc
     2461 gccggatcgt cggcgcctat gaaacggcgg cggctttaaa ggcaaagacg gaacggcaat
     2521 gaaccccgta atcgacattg ccgtcgaggc cgaggaatgg acgcagcttg aggaccatcc
     2581 gaggctcgtc gaggccgcga tccttgcggc agtcggcgaa agtggcattt ccctcggccc
     2641 aaatgccgaa gtcagtgtcc tcttgtgtga tgatgcctac atccagaaac tcaatcagaa
     2701 atggcgcggc atcgacaagc caaccaatgt gctgtccttc cccgcccctc atgatgtggg
     2761 gtcatcgggc ctgctcggcg atatcgtcat cgccttcgag accgccgcga aggaagccgc
     2821 cgcagctcaa attccgttac acgaacacgt cgcccacctg ttggtccatg ggtttttgca
     2881 tcttgctggt catgatcata ttaagacgct agaagccgag gcgatggaaa tgatcgaacg
     2941 cgctgcgctc ggcagactgg gaatttccga tccctatagc gcgtcgttag ttgaggaagc
     3001 agcctgcgcc aatgaacgag catgacactc ctgctggaac cgaggcggcg aagttgggtt
     3061 ctccgggaaa gccgggtctg tttgaccggc tgcgcgcgtt gttcggcctc ggcgggatgt
     3121 caacgcgtag cgacatcgaa gtcgttctgg ccgatacttc gatcgaggac gatatttctt
     3181 cgcacgagcg cgcgttgctg aagaatgtgc ttgccttgca cgaggtgcat gttggcgatg
     3241 taatgattcc gcgcgccgat attgtcgccg tttcattgca agccaagctc gccgatgtgc
     3301 tggaggtctt tcgctcggcc ggtcattcgc gcctgcctgt ttacagcgag acgctcgacg
     3361 aaccgcgcgg catggtacac attcgcgatt ttgtcgccta tctcaccgct gccgcaaacg
     3421 agcctcacgg gaaagtatgc gctgatggcg agtcgcctgc tgagcctcag agcgattcag
     3481 ttagagcggg cggcttcctg cggcgcgacc gagacatgac cttatcggaa gccaatattc
     3541 tgcgcccggt gctttttgtt ccagcctcca tgccggcgct tgatcttctt gtgaaaatgc
     3601 aagctacgcg aacgcatatg gcgctcgtta tcgatgaata tggaggcacc gaaggtcttg
     3661 cctcgataga agacatcatg gaaatgatcg tcggggatat cgaggacgaa cacgatttgg
     3721 acgagggtcc gaagatcgag accagaccag gaggtcattt cgtcgcagat gcgcgcgccg
     3781 gtctcgaaga ggtgtcgagt gcaatcgggg ccgatcttac cgcgattagc gatgctgaag
     3841 aggtcgatac tttggggggg ctcatcataa ccctcgcggg gcatgttccg gcgcgcggcg
     3901 aaatcataac cggagatggc atcgaatttg aaatcgtgga cgccgaacct cgccgcgtca
     3961 agcgggtcca gatccgtatc gcgggaacgt cctctgctgc tgcagacatt gagaaaaggc
     4021 tgcctccaga agagcccgct aggcaagcct cccttccgcc aaggcgctag ctgacacccc
     4081 gacaaaatgg accgcttttt ctcggtgcgc tgttcgtctt tattgaccgc tccggcagca
     4141 tcttcaactt tttgcagccg atcgacgcgt agccgaatgc cactgtcacg cgcgccgcgg
     4201 tttcgccgca agcagcatac cgccacctca acgagattgt cacggccgcc gaggcgcacc
     4261 ccaattctat ccaaagagct ctcagggtta tgcttaagag gcatgtccat gtctcgtatt
     4321 gtctggagca agactcgtgt cattttgccg gccccgtgcg aggaacctgc gagagcgtgt
     4381 tgagccaaaa gtagaactcg gttttgcgcc cgggtgacaa tacaaagaac tagagcaaga
     4441 tgacgattcg aagaaaagtc atctcgctct agactcacga atgaggacga aaaagcccct
     4501 catgctcgcg atcgccactg aagtcaccct cgcctctgga tggtgccgat cgatcattgc
     4561 ctttgtgacg ggcgccacag gcgcgtttgc catggcgccc ttcgactttt ttccagcgct
     4621 catgattccg atgactgctg ccatctggct catcgacggt tgcgcggaga tacggcaaaa
     4681 atcgcgcttc ccgtggatga gcctgggatt accgctggcc gcgtggcgcg ccttctctgc
     4741 cgggtggtgg tggggctttg gttatttcac tgctggtctt tggtggctcg gcgcagcttt
     4801 ctttgtcgat gctgaggaat ttgcctgggc cttgccgctt ggcgtggcag gacttcccgc
     4861 agtcctggcg gtcttctcgg ggctcgggtt tatgctggcg cggctcgtct ggctccccgg
     4921 cgcgggccgg ctttttgcct ttgctgcggg attaagcttt gcggaatggc tgcgcgggca
     4981 tctcttcaca ggatttccgt ggaacgtatt cggcatggcc ctaggcggca atctcgtgac
     5041 cgcgcaactc gcctcggtcg ttggccttta cggactgacg attgttgcga tccttatctt
     5101 ttctgctccc gccttcttcg gcgacaaacc caatgccagg tggatcgtct gccgcgttcc
     5161 tttacccgtc gccgcagcgg tcctggcctt tgctggaatt tgcggcttcg gcgctctacg
     5221 tctttcggag ccaccgccga agatgatttc cggcgtaaac ttgcgcatta tgcagcccaa
     5281 tgtggcccag gacgaaaaat tccggccaga aaacggcgct gagattttag cccgttatct
     5341 cgcattgtcc gtgcgcgaac cattaggcct cgatggggtc agtttgctca tctggccaga
     5401 gtcggcattc cctttcatct tgtcgcgctc cccctgggca ctttcggaaa tcggcgaggc
     5461 cttatcgaaa aacacgctcc tcgttaccgg cgccgcgcgc gcggacggcg gggcgcgaac
     5521 cgtcggacgc cctcattacc tcaacgcagt ccaagtcatt gcgagcggag gacatatcct
     5581 tgacagctac gacaaggtgc accttgtccc ttttggcgag tatttgccct ttcaatccat
     5641 tttcgatcgc ttgggcctgc gacagtttgt gcatatcccg ggcggcttcg aagccggagc
     5701 tgggccgcgg ttattggttg tcccgggcct gccctctgtc gtgccattaa tttgctacga
     5761 agcgatattc ccgagcgagg ttgtaccaag aggggcggcg cgggaacgcc caggcgcgct
     5821 tctaaacgtt accaacgacg gctggttcgg ggagacgccc gggccttacc agcattttgc
     5881 ccaggcgcgg ctgcgcgcga tcgaagaagg cttgcctttg attcgtgcgg ccaactcggg
     5941 tatttccgcg attgtcgatc cctacgggcg aattttggct aaactccccc tgggcgtgga
     6001 aggcgtgctc gacggcggct tgccgcaaag cattgccgcg ccgccatttg caggttttcc
     6061 tttcacaatc gtttcattat tttgggtttt agtgcttagt ttcgccttgg ccgtcagatt
     6121 ccgtgaacta aacgccgatt gatgctaaga gcaagattgt gtttcttacg aattaacatc
     6181 aatgtgtttg ttacgaatta atatctaact gtgcttttt
//
LOCUS       DATMPU010000151         4442 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_2289417,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000151 DATMPU010000000
VERSION     DATMPU010000151.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 4442)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4442)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4442
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2289417"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..657)
                     /locus_tag="VNY06_03540"
     CDS             complement(<1..657)
                     /locus_tag="VNY06_03540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012591140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HWX13931.1"
                     /translation="MAHIWLPYTQMRAAAPPLPVVRTQGSRIYLADGRGLVDGIASWW
                     TACHGYNHLHIRDAVSRQLGLMPHVMFGGLVHEPALALARRLAALLPEGLDRVFFCDS
                     GSVAVEVAMKMAVQFWLNQGNRQRNRFVAFRGGYHGDTFGAMSVCDPDEGMHSLFARV
                     LPQQIITELPLTASTAEVFDLLLERHAEDIAGIIVEPLVQGAGGMVFHAPNMLRHLRA
                     A"
     gene            complement(912..1136)
                     /locus_tag="VNY06_03545"
     CDS             complement(912..1136)
                     /locus_tag="VNY06_03545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX13932.1"
                     /translation="MAIVNPTAPGTWLQLRAAAESLVILVAMIVVFGAAAYSTGSLYG
                     RPGLISIVAGFAVVVAALFLLGGLRFGYLR"
     gene            1586..1858
                     /locus_tag="VNY06_03550"
     CDS             1586..1858
                     /locus_tag="VNY06_03550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020176739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-finger domain-containing protein"
                     /protein_id="HWX13933.1"
                     /translation="MATHTTPHFHNQPGVPQVRIGAKEFMCVGALPPLDHPHIFIDMG
                     DDKDAICPYCSTHYVYDASLHGGCEPAECVFEPSAETAVEPEHLQV"
     gene            1877..3076
                     /locus_tag="VNY06_03555"
     CDS             1877..3076
                     /locus_tag="VNY06_03555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012590021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HWX13934.1"
                     /translation="MSPPHAVIAGAGIGGLCTAICLARRGWRISLYEQAKFLEEVGAG
                     LQLSPNASSILRELGIIDHLARFAICPKAIIIRRARDGATLARMPLEGAEQRWSAPYL
                     VVHRADLQRVLLDAAARTSAISLTTDAAVNGIEPGENSVSVVIRRGAVSLKEGSDCLI
                     GADGVRSFVRQSLGLGSARFSRKTAWRALLEAVCVPAAMRRLETTLWVGRGAHLVHYP
                     LRGGAVINVVAIVDENFYSGEESFWSSLGDPRFLEARFENWDGAARELLRAAPEWRKW
                     PLFDCRPIANWVSGRVALIGDAAHAMLPFLAQGAAQAIEDAAALSAALDQGKDIESGL
                     LSYQAMRHARATRVQRESRRQAAIYHLAGPAAFLRDVTLRALSGPKMLARYDWLYGAG
                     NATRRDT"
BASE COUNT         1004 a         1234 c         1297 g          907 t
ORIGIN      
        1 tgccgccctc aaatgccgca acatgtttgg ggcatgaaac accatgccgc cagcgccctg
       61 caccaaaggc tcgacgataa tgccggcaat atcttccgca tggcgctcaa gcagcaaatc
      121 aaaaacttcg gcggtggagg cagttaaggg gagttcggtg ataatttgtt ggggaaggac
      181 tcttgcaaaa aggctgtgca ttccctcgtc gggatcgcag accgacatgg ccccaaaagt
      241 gtcgccgtga tagccgcccc taaatgcgac aaagcggttg cgctggcggt tgccctgatt
      301 gagccagaac tgcacagcca ttttcatcgc gacctcaacc gcgaccgagc cgctgtcaca
      361 gaaaaagacc cggtcaagcc cctcgggcaa tagagccgca aggcgacgcg cgagagcgag
      421 cgcaggctca tgcacaagac cgccgaacat cacatgtggc atcaggccaa gctgacgcga
      481 aacggcatcg cggatatgca ggtgattgta accgtggcag gcagtccacc agctcgcgat
      541 cccgtcgacg agcccgcggc catcagccaa ataaattcgg cttccttgtg tgcggaccac
      601 aggcagtggc ggagccgccg cccgcatttg tgtatagggc agccaaatat gcgccatgcc
      661 gcgggcaaac cagtccggtg gagcgctttt cacttgatcg tgcctgagga agatggtgcc
      721 gcctagtctt tcgcaataac tatagctgcg attcgctccc tttcgccatc ttcgaacaaa
      781 ataggatgtt tgcaagcgcg gtgcgttggg agtggattct cgcacagaga ggagaagtca
      841 tgacgagggg ggagagcggg aggccaagaa cggtgtggcg cccgcctgcg ccacgccgga
      901 gatgtctgcc gctaccgcaa atagccgaag cgcaaaccgc cgagcaaaaa gagcgcggca
      961 acaacgaccg cgaacccggc aacaatcgaa attaatcctg gcctcccgta caagctgcca
     1021 gtagaatatg ccgcggcacc aaagacaacg atcattgcga ccaaaattac caggctttca
     1081 gccgctgccc tcagttgtag ccaggtgcca ggcgccgtgg gatttacaat tgccatagtc
     1141 gatctccctt gttcatttct gcctcttctg gattcggttc ggtgcgctac ttgtccgttt
     1201 cttggtatgt gcgatttgga cgtaccggcc tgccgaattt cttcaagacg tgttgaacag
     1261 tatgacggca gcattatcgg ggcttgatct ggatcaattt aacgaggcgt caggcaacgg
     1321 cctattgacg cagatggcgt attgacgcag atggcgtatt gacgcagact tgcatactaa
     1381 accgcggcgc ggtgccatgc tcgcaaattg tgagatcttg ggcttttaag aattttggat
     1441 cgatcgtcca taatgagacg gggatgtctg acgtccaacg gctatccaaa cccagctcgg
     1501 cagattattt tgctaggctt gttggggaac cgaatttacc aaaaggtaac gccttcaaca
     1561 ctatcgacaa cagatcgaga ctatcatggc cacacacacc acgccgcatt tccacaacca
     1621 gccaggcgtt ccgcaagtgc ggatcggcgc caaggaattc atgtgcgtcg gcgcgttgcc
     1681 gcctctcgat catccgcata ttttcatcga catgggcgac gacaaggacg cgatctgccc
     1741 ctattgctcg acccactatg tttacgacgc gagtctccat ggtgggtgcg agccggcgga
     1801 atgcgtgttc gagccgagcg ccgaaacggc ggtggagccc gaacatttgc aggtctaagc
     1861 cgcaaatgca ggccctatga gcccgccgca tgcggtaatc gcaggtgcag gaatcggcgg
     1921 gctatgcacg gccatttgtc ttgcgcgcag aggatggcgc atcagccttt atgaacaagc
     1981 aaaattcctc gaagaggtgg gtgctgggct gcagctctcg ccgaacgcca gctcgattct
     2041 gcgagaactt ggaataattg atcaccttgc gaggtttgcg atttgcccga aggcgatcat
     2101 catccgccgg gcgcgggatg gcgcgaccct cgcgcggatg ccactcgagg gcgcggagca
     2161 gcgctggagc gcgccttatc tcgtcgtgca tcgggccgac cttcagcgcg tgctcttgga
     2221 tgccgccgca cgcacctccg ctattagcct cacgaccgat gctgcggtta atggcattga
     2281 gccaggcgaa aacagtgttt cggttgttat caggcggggc gcggtttctt tgaaagaagg
     2341 gagcgattgt ctgatcggcg ccgatggtgt ccgttctttt gtacgccaaa gtcttggact
     2401 tgggagcgca agattttcgc gcaaaactgc ttggcgcgcc ctgctcgagg ctgtctgcgt
     2461 tccggctgcg atgcgccggc tggaaacgac cctttgggtc gggcgcggcg cgcatctggt
     2521 ccattatcct ctccgcggcg gcgccgtcat aaatgttgtc gccattgtgg acgagaattt
     2581 ttactccggg gaggagagct tctggtcgag tcttggggat ccccgcttcc tcgaagcacg
     2641 ttttgaaaat tgggacgggg cggcacgaga gttgctgcgt gcagcgcccg aatggcgcaa
     2701 atggccgctt tttgattgcc ggccgatcgc aaactgggtc agcggccggg tggcgctgat
     2761 cggcgatgcg gcgcacgcaa tgctgccgtt tctggctcaa ggcgcagccc aggccatcga
     2821 agatgccgcc gctctgagcg cagcgctgga tcaaggcaaa gacatcgaat caggtctcct
     2881 cagctatcaa gcgatgcggc acgcgcgggc cacccgcgtg cagagagagt cgcgcaggca
     2941 agccgcaatc tatcatctcg cggggcccgc ggcattcctt cgggatgtaa cccttcgggc
     3001 gctaagcggc ccaaaaatgc tcgcgcgcta cgactggctt tatggcgcgg gtaatgccac
     3061 aaggcgagat acatgacggg caatggttcg caggcgctct cttcaacata ttggcgcgat
     3121 ttctttcttt ggctgaggtg ctcaaggctt ccgaagcccg acgcgtgaca gaccacgcgg
     3181 agcgcagcgg tatcgtttct tcctgaccga agtagcaaca tagagttgcg cctggagcga
     3241 catgactttt cttcgaaacg tcatctcgta gtagatctgt gtcaggacac aaagtgaata
     3301 ctcactttgc agcacatgct ctaaaggccc ggaaaaattc cgttctcaat gagaactgtc
     3361 cagccgaaac cgcccattag caataggact gccctgacga gcggctgctg cgagaaaagg
     3421 cccaaatgca ccctcgctca tttgatcgac atgcctcagc aagagctcga gctgctgcgc
     3481 cgttttcgat agcgcctctg acgcggccag tgttattggc cccgttgcct ctgcggaaag
     3541 agttgcattt acgagatcgt gcttggccgt agcgcgtccc gtgttcgcct ccgagtaaag
     3601 cacaatggcg caggcaatta caacagcgag gctcgctccc accgcaatga aactagacag
     3661 attgggcgga taattggcga aggccgtact gctgttactg cgagcaacgc aatcaataca
     3721 aaaggatagt aaataagcga gccgatgcag cgggtgcgcc ttgcgaccaa ctccaagttg
     3781 atccaatcac ggacaacgtc ttcttggagc tgcaatcggc cattgaatag actcgttgtc
     3841 tcggctggcc atttacactt agcgcggcga agctttctca caaaatgcag gcgaccgtat
     3901 gttgcgtcga atacaaagca agtcaaaaac accatgagca gggtacaaag gaaggtagtg
     3961 cagaaatatg cgcttgcggc aagcgctcct cgagcgggga tggaaggatt gccgaacaat
     4021 ggaacgacga acagtgtaac gatcaaacag atgacagcgc tataggagac tgcgcgacaa
     4081 actcggggcc agaatgattc ctgaccgacg taggatctcc aaattgtctg aacttttacg
     4141 ggcgtagagt gggcggtctt accatttcca gttgaatagt cgaaggcagc gacaatattg
     4201 gtccacaatg agcgcaggtc atgggaccct ggggcggtca tatccaatct gagctcatca
     4261 ccgatgctct cgaggttgtt gtggaggctg cgttgcgcct gccaggtcaa gtaaatggca
     4321 aggacaattc ctaagctgcg aagcagcact gtcggccaga cgcttacgcc cctgaagcag
     4381 ggctatcggt tcacgattgc catgctccgt cagaaattgc gcgagcggct cccagaaggc
     4441 gc
//
LOCUS       DATMPU010000152         2903 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_2289876,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000152 DATMPU010000000
VERSION     DATMPU010000152.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 2903)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2903)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2903
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2289876"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2667)
                     /gene="dnaE"
                     /locus_tag="VNY06_03560"
     CDS             complement(<1..2667)
                     /gene="dnaE"
                     /locus_tag="VNY06_03560"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020175859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HWX13935.1"
                     /translation="MLADIYLAEFGTRMTADGLITEAGGPGAGEPERIIIETGFVHLH
                     VHSSYSLREGAIPVGKLGKLAVADSMPALAITDSNNLFGALEFSEKLAKEGVQPIVGL
                     QIAVDFQDGVPASRSETKTNQASIVLLAMDALGYANLMHIASRLWLDPQAGEPPHITI
                     VGFEGKTEGLIALTGGPNGPLDQAFAHGRAERAFSRAATLERLFRDRIYVELQRHGLD
                     CERAVEPQLLDLSYRASLPLVATNEVFFAAASDYEAHDALICIAESTVVSDGTRRQLS
                     PEHRFKTRAEMLALFSDLPEATRNSVEISLRCSYRPLTRKPILPRFSVDSGADGEAQE
                     LRAEARAGLEARLAARGPARGFTTKDYHARLAFELDVIIKMNFPGYFLIVADFIKYAK
                     SRDIPVGPGRGSGAGSLVAYALTITDLDPMQFGLLFERFLNPERVSMPDFDIDFCQEQ
                     RDEVISYVRRRYGADRVAQIITFGSFLARGVLRNAGRVLEMPLGQVDKLAKLVPQNPA
                     SPVSLKQAIESEPRLAEAAEADPRVFKLLQIAQALEGLYSNASTHAAGIVIADRPLEE
                     LVPLYRDPKSDMPATQFNMKWAEAAGLVKFDFLGLKTLTIIAKCVKLLQRRGIAVDLA
                     QIPLDDKKTFAMLERGETAGVFQLESAGMRKALTEMRADRFEDIIALVALYRPGPMAN
                     IPAYCAVKRGEEEPDYIHPKIAPILKETFGVIIYQEQVMQIAQVLSGFSLGEADLLRR
                     AMGKKIKAEMAAQRTRFITGAIERGLDKGKADEIFDLLAKFADYGFNKSHAAAYALIA
                     YQTAWFKANYPVEFLASSMTLDKSNTDRLAEFCNEARRLSVKVLPPSVQTSGPDFEVE
                     MSDGGELCIRYALSAIKGVGDGHAKALAAINRI"
BASE COUNT          625 a          827 c          816 g          635 t
ORIGIN      
        1 tatgcgattg atcgcggcaa gtgctttcgc atggccatcg ccgactcctt tgatcgcaga
       61 caatgcatac cgaatgcaga gttcccctcc atcactcatc tcgacctcga aatcgggtcc
      121 tgacgtttgc accgaagggg gcaagacctt gacgcttaga cgccgcgctt cattgcagaa
      181 ctcggcaagt ctgtccgtat tcgatttatc gagcgtcata gaagacgcca aaaattccac
      241 cggataattg gctttaaacc atgcggtctg ataagcgatc agcgcgtaag cggccgcatg
      301 acttttgttg aagccataat cggcaaattt ggcgagaaga tcgaaaatct catctgcctt
      361 ccccttatca aggccgcgtt cgatggcacc ggtgatgaac cgcgtgcgct gtgccgccat
      421 ttctgccttg atcttttttc ccatagcacg ccgcagcaga tccgcctcgc cgagagaaaa
      481 cccggagagc acttgggcga tctgcatcac ctgttcctga tagatgatga caccgaatgt
      541 ctctttgaga attggcgcga tcttgggatg tatatagtcc ggctcttcct cgccgcgttt
      601 taccgcgcaa taggctggaa tattggccat cgggccaggc cggtagagcg caaccagagc
      661 aatgatatct tcgaaacggt cggcccgcat ctcggttaag gctttgcgca tgcccgcact
      721 ttcaagctgg aaaacgccgg ccgtctcgcc gcgctcgagc atggcgaagg tctttttgtc
      781 atcgagtgga atttgcgcaa gatcaacagc aattccgcgc ctttgcagca gcttgacgca
      841 tttggcaatt atcgtcagcg ttttgaggcc gagaaaatcg aacttcacga gtccagctgc
      901 ctcggcccat ttcatgttga actgggttgc cggcatgtct gatttcggat cgcgatagag
      961 cggcacgagt tcttcgagcg gccggtcggc gatgacgatg cctgccgcat gggtcgatgc
     1021 attcgaataa agcccttcga gcgcctgcgc aatttggagc aatttgaaaa cgcgaggatc
     1081 cgcttcggca gcctcggcaa gacgcggctc ggactcgatt gcttgcttga ggcttaccgg
     1141 agatgccgga ttctgcggaa cgagcttggc gagcttgtcg acttgaccca agggcatctc
     1201 gaggacgcga cccgcattgc gcagaacgcc gcgcgcgaga aaggaaccaa aggtaatgat
     1261 ttgtgcgacc ctgtccgcgc catagcggcg gcggacgtac gaaattacct cgtcccgctg
     1321 ctcttggcag aaatcgatat cgaaatccgg catcgacacg cgttcgggat taagaaagcg
     1381 ctcgaacagg agcccgaatt gcataggatc aagatcggtg atcgtaaggg cataggcaac
     1441 gagggagccc gctccggacc cgcggcccgg tccgactgga atatcccgtg atttggcata
     1501 tttgatgaaa tccgcgacga tcaggaaata gccggggaaa ttcattttaa tgattacatc
     1561 gagctcgaag gcaagtctcg cgtgataatc tttcgtggtg aagcctcgtg ccgggccgcg
     1621 cgctgcaagc cgcgcttcca gtccggctcg cgcctccgca cggagttcct gcgcctcgcc
     1681 atcagcgccg ctatcgaccg aaaagcgcgg cagaatcggt tttcgcgtca gcggccggta
     1741 gctgcatctg agcgagatct cgacgctgtt tcttgttgcc tctggcaggt ccgagaacag
     1801 cgccagcatt tcggcccgtg tcttaaatcg atgttcgggc gaaagctggc ggcgcgtgcc
     1861 gtcgctgact acggttgatt ctgcaatgca gatcagggca tcatgcgcct cataatctga
     1921 agccgcggca aaaaatactt cattcgtggc aaccagcggc aaggaggcgc gataagaaag
     1981 gtcgagcagc tgcggctcga ccgctcgctc gcagtcaagt ccgtggcgct gaagctcaac
     2041 ataaatccgg tcgcgaaaaa gccgctcaag cgtggccgcg cggctaaatg cgcgttccgc
     2101 tcggccgtga gcaaaggctt gatcaagcgg cccgtttggc cccccagtca aggcaatcag
     2161 cccttccgtt tttccttcaa agccgacgat ggtgatatgc ggcggttctc cggcttgcgg
     2221 atcaagccat aggcgcgagg cgatatgcat cagattcgca tagcctaaag cgtccattgc
     2281 caagagaacg atcgacgcct gatttgtctt cgtctcgctg cgcgacgctg gcacaccgtc
     2341 ttgaaagtcg acggcaattt gcaggcccac gatcggctgc acaccctctt tcgccagttt
     2401 ttccgagaat tcgagggccc caaacaaatt gttcgaatcg gtgatcgcga gcgccggcat
     2461 ggaatcggcg acggcaagct tgccaagctt gccgaccgga atcgccccct cgcgcagaga
     2521 gtaggagctg tgcacatgga gatggacaaa tccggtttcg ataataatgc gctctggctc
     2581 accagcgcca gggccgcccg cctcagtgat taagccgtct gcggtcattc tcgttccgaa
     2641 ctccgcgaga tagatgtctg ctagcactat gcaatctgtg cctttggcac gcgtacattc
     2701 tgcgggcaac ggtcttcgcc acggatcttt ccctcaattt tagactagag cgtgtccggc
     2761 aaaaagtgga ttccggttgt cccggatttt agagcaagac ggcgattcga agaaaagtca
     2821 tcttgcgcta cggggttttt taccggcctt gcccggcgaa gccttactcg tggcatcatg
     2881 gccttaacgg tcgcccgcgc aat
//
LOCUS       DATMPU010000153         3833 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_3533791,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000153 DATMPU010000000
VERSION     DATMPU010000153.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 3833)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3833)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3833
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3533791"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2875)
                     /locus_tag="VNY06_03565"
     CDS             complement(<1..2875)
                     /locus_tag="VNY06_03565"
                     /inference="COORDINATES: protein motif:HMM:NF024516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3971 domain-containing protein"
                     /protein_id="HWX13936.1"
                     /translation="MIEVSSFSLLLKGSEQLASLGEEILARARMWLKNWPRFCEDAGA
                     WIRRNANRIPGVAWHSYFLPKVVLGVFSVVLLVVILFAVRLSLGSIPIDSFAPRIASA
                     IGERIGHGYEFNIGRMAIAFDGVVPVLSIDELSAKEPTGRNILTAPRAEISVGPLALM
                     AGRVSLKRLEIYDVELRLALRPNGSLALPVSPEIGDAVDLTPPLRSPGSPDGALLSSP
                     EAPPALRGDTGELHRVPPESQFAAMLRLAIDTLTSPDSVTAAIRHIGITRGKIIVDDE
                     TASQKLVFNGVNLGFDRSSRSTRFDLSVDGPNGRWRAFGAASETEGLERSLTLSVANL
                     SLDELLLAAGTRTIGVDFDMPLSGDLDIRLGADGMISQAAGKFESGSGYFRFDDPDGE
                     PTMIDRMGGGFHWNEARRRIVIDRLQMAAGATHFGGSGSVAPPARDGDPWSIQLANTE
                     PNVWGAERLGENPILIDLTDLAARLYLTEKKLAIDRFSFSGPQCGLALAGTIDWVAGP
                     HVRLGASISPTPVKTVARLWPSLIAASVRSYLLSHASEGTVESGTVQIDFDAADLRAI
                     REEHAPADAKAVVEFEIKNASLAFLPGVPPLRGIDGIGHITGRTATFTVSNAAIDSGN
                     GRVLTLWDGSFRVDDTDLKPVPAVARGKVMGSVEAIGELLSHDALKPYASLPLDSSTL
                     KGEANGTLEIDMRLGANTGPSDTKLKIDALASNLTAERLIGNEKLEAATLTVNVDPTG
                     LRASGQGTMFGAPATVGIEKLAGKPAEASIGMNIDDAMRAKLGIGALSGVSGPIGAKI
                     TAPLGTGEKPKAKIELDLSAAAIEIPGISKPAGQPGKISFAVAVNDASTSLDQIIVDA
                     GPVQGHGKMELGTGFSLITASFPQVRLSSGDELRVDATGAGDTLKVVVRGTSIDARPF
                     LKSLIFDPPGSAKAEATMGEERDKAGQGKETGQGKEIEFDVKSD"
     gene            2974..3498
                     /locus_tag="VNY06_03570"
     CDS             2974..3498
                     /locus_tag="VNY06_03570"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012383029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HWX13937.1"
                     /translation="MHENLPKKPPKPSDKGSDGGVRLVPGDKAPDFDLLDDAGGKVTL
                     AGFRGQLLVLYFYPKDDTSGCTKEAIDFNGLQSEFEKAGAAILGVSADSAASHARFKA
                     KHKLEIALGSDDSKAMLMAYGVWVEKSMYGRKYMGIERTTLLIGKDGKILAVWNKVKI
                     PGHVAAVLAAVKAH"
BASE COUNT          846 a         1160 c         1054 g          773 t
ORIGIN      
        1 tatcggattt cacgtcaaat tcgatttcct tgccttgacc tgtttccttg ccttgacccg
       61 ccttgtcgcg ctcctctccc atcgttgcct cggccttagc gctgccagga ggatcgaata
      121 tcagcgattt caggaacggt ctcgcatcga tcgaggttcc ccgcaccacg acctttagtg
      181 tgtctccggc gcccgtcgca tcgaccctca actcgtcgcc ggacgaaagc ctcacctgcg
      241 gaaaagacgc cgtaataaga gaaaacccgg tgccaagctc cattttgccg tgtccttgaa
      301 ctgggccagc atcgacgatg atctgatcga gcgaagtcga agcgtcattg acagcgactg
      361 cgaatgaaat cttacctggc tgacctgccg gtttcgagat tccgggaatc tcgatcgcgg
      421 ccgcgctgag atcgagttcg attttggcct tgggcttttc cccggtaccc aggggcgcgg
      481 tgatcttggc acctatcggt ccactcacgc ccgacagtgc accgatccca agttttgcgc
      541 gcattgcatc gtcaatattc atgcctatcg acgcttcggc tggcttccca gccaatttct
      601 caatgcctac cgtcgccgga gccccaaaca tggtaccctg tccgctcgct ctaagtcccg
      661 ttgggtccac attcacggtc aacgtggcgg cttcgagttt ctcatttccg atcaaccgtt
      721 cggcggtgag attgctggcc agcgcatcga tcttgagttt tgtgtccgat ggacctgtat
      781 tggcgccgag cctcatatca atctctaacg taccgttcgc ctctcccttt agcgtagagg
      841 aatcgagcgg cagactcgca tagggcttta aagcgtcatg agagagcagc tccccaatcg
      901 cctcgacgct acccattact ttgccccgcg cgacggccgg gactggcttt aagtcggtat
      961 cgtcaacacg gaaactgccg tcccacaagg tcagcacccg gccattgccg gaatcaattg
     1021 ccgcgtttga tactgtaaaa gtcgcagtgc gccccgtgat atggccaatt ccgtcaatcc
     1081 cgcgcaaggg tggcacacca ggcaggaacg cgaggctcgc attcttgatc tcgaattcaa
     1141 ccaccgcctt tgcatcggct ggcgcgtgtt cttcccgtat cgcccgcaaa tctgctgcat
     1201 cgaagtcaat ttgaaccgtt cccgactcca cggtgccttc gctggcatga gaaagcagat
     1261 aggatctaac cgatgcggcg atcaacgaag gccacagtct tgcaacagtt ttgacaggtg
     1321 tcggagaaat cgacgcccca agcctgacat ggggtccggc tacccaatct attgttccgg
     1381 cgagcgccaa accgcactgc gggccgctga acgaaaacct gtcaatcgcg agtttctttt
     1441 cagtgagata aagccgagcc gctaggtctg tcagatcgat caatattgga ttttcgccaa
     1501 gccgctccgc gccccacaca ttgggttccg tgttggccaa ttgaatcgac caaggatcgc
     1561 catctcgggc cggcggcgcg actgagcctg acccgccgaa atgtgttgcc ccagccgcca
     1621 tttgcaagcg atcaatcact atgcgccgcc gcgcctcgtt ccagtgaaac ccgccgccca
     1681 tcctgtcgat catcgttggt tcgccgtcgg ggtcgtcgaa ccggaaatag ccagacccgg
     1741 actcgaactt cccggcagcc tgcgaaatca tcccgtcggc ccccaatcgg atgtcaagat
     1801 cgcccgacaa aggcatatcg aaatcgacgc caatcgtgcg cgtgccggca gcgagcagga
     1861 gttcatcgag agacagattt gcaaccgaaa gcgtgagcga gcgttctagg ccttcggtct
     1921 cgctggccgc gccaaacgcc cgccaacggc cgttcggccc atcgactgaa agatcgaacc
     1981 ttgtcgacct ggaggatctg tcaaacccaa gattaacccc attgaaaacc agtttctgac
     2041 tcgcggtctc gtcgtcgact atgatcttac cgcgcgtgat gccgatgtga cggatcgcgg
     2101 cggtcacact gtccggactt gtcagcgtgt cgatggcaag ccgcaacatc gccgcaaact
     2161 ggctctcggg cggaacgcga tgcagctctc ccgtgtcgcc ccgcaaagct ggcggcgcct
     2221 caggcgagga caagagagcg ccatctggcg aacccggcga acgcaacggc ggggtaagat
     2281 cgactgcgtc cccaatctcg ggcgaaaccg gcaaggcaag tgaaccgttg ggccgcaagg
     2341 ccaagcggag ttcaacgtca tagatttcaa gccgcttcag acttacccgc cccgccatga
     2401 gagcaagagg gccgaccgaa atttcggcgc gcggtgcggt taaaatattt cgcccagtgg
     2461 gttccttcgc cgacaattca tctatgctca gcactggcac gacgccgtcg aatgcgatcg
     2521 ccatcctgcc aatgttgaat tcatagccat ggccgatgcg ctcgccgata gcgctggcaa
     2581 tacgcggtgc gaaactatcg atcggaatag atccaagcga gagtcgaaca gcaaatagga
     2641 tgacgacgag aagcactacg gagaaaacgc ctagtacgac tttgggcaaa aagtaggaat
     2701 gccacgccac accgggaatg cggttagcgt ttcggcgaat ccatgcccct gcgtcctcgc
     2761 aaaagcgtgg ccaattcttc agccacatgc gcgcccgagc aagaatttcc tcaccgagac
     2821 tggccaactg ctcgctgcct ttcaatagca ggctgaagct ggaaacttcg atcacccttg
     2881 cctataggca ccttatggca aaatcgcgcg acgcaaactg acacgggcaa ggtttctgcc
     2941 aattgccgct taatatgacg aaaggaaggc tccatgcacg aaaatttgcc taaaaagccg
     3001 ccaaagccat cggacaaagg ctcagacggc ggcgtaagac tggttcccgg cgacaaagcg
     3061 ccggatttcg atctgctgga cgacgctggg ggcaaagtta cactcgcagg ctttcgtgga
     3121 caattgctcg ttctttactt ctacccgaag gacgatacct cagggtgtac aaaggaggcg
     3181 atcgatttca acggcttgca gagcgaattt gagaaggctg gcgcggcaat ccttggcgtc
     3241 tcggcggatt cagcggcgag ccacgcgaga tttaaagcca aacacaagct tgaaatcgcg
     3301 ctcggctccg atgactcgaa agcaatgctc atggcctatg gagtctgggt cgaaaagagc
     3361 atgtatggcc gcaaatacat ggggatcgag cgaacgacgt tgctcatagg gaaagacggc
     3421 aagatccttg ctgtttggaa caaggtgaaa attcccggac acgtggcggc agtcctcgcc
     3481 gccgttaagg cgcattgaag cggcgtcggt taagcggtcc gttcccccgc aattgccttt
     3541 tgctggcgga aggagcaaac gatctagctc aagctggcaa cgcgagcgcc ttgcggttcc
     3601 tcgggggcgc tccgcggccc ccacagaacg gcggccagca agctacaggg ggcaacctgt
     3661 ccggcgggca gacgacggcc gaagcaggtt cgaggctgct tcgaaaacat ctgatcgtcg
     3721 aaacttcatt ctgctttgtc cgcaaagagc gcggccattg cattgccgcc gctgcagagc
     3781 gagcctatac cggcgtgagg ccgcatgcta aggggcgcgc cttagcatgc ggc
//
LOCUS       DATMPU010000154         4946 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_2601947,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000154 DATMPU010000000
VERSION     DATMPU010000154.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 4946)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4946)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4946
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2601947"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            167..568
                     /locus_tag="VNY06_03575"
     CDS             167..568
                     /locus_tag="VNY06_03575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020174119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2794 domain-containing protein"
                     /protein_id="HWX13938.1"
                     /translation="MKTEENPVTHPPRSRKDPFAQPASRISPAPPGIADVISFNRDEL
                     RKIFSLYGRKVGDGEWQDYAIDFTPQKAVFFIYRRACEYALFRIEKCPRLAQKQGAYS
                     VITATGLILKHGHDLSDVIAALDSGLKLVSG"
     gene            1070..1681
                     /locus_tag="VNY06_03580"
     CDS             1070..1681
                     /locus_tag="VNY06_03580"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020176938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HWX13939.1"
                     /translation="MANRLPINECAEPSDTGLGRSDLPPPLAIVAAIGENGVIGDGRG
                     LPWHLPSDLKHFREITMGKPMLMGRRTLESIGRALPGRETIVLTHDRAFKPPHSVGHD
                     GLIHVAYDLETALALAQARAKMLGAEEVILAGGGNLYESLIDRAKRMHLTLVDLAPKG
                     RVRFPVIDWSNWLETCRVRPRPTAKDEATFAFVDFDRRGCLLL"
     gene            1777..2895
                     /gene="hflK"
                     /locus_tag="VNY06_03585"
     CDS             1777..2895
                     /gene="hflK"
                     /locus_tag="VNY06_03585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012589646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0045861 - negative regulation of
                     proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HWX13940.1"
                     /translation="MPWSNEGGNGGSWKPSSPGPWGQKPGAPPPEFEEFLKRAQEKLK
                     QWIPAPGGMGGRSLLALALVGTIIWLFSGFYTVGPNEVGLKKIFGRYISKTGPGLNYN
                     FPFPVGSVEKLQVTDRNTINIGFAFRQDVRTPGQTQLDLPEESLMLTGDENIADVKFV
                     VIWQIDPVRPEDYAFNISNQRDTVKAVAESAMREVIGRGQIQRILTAERQIIETDVQE
                     LMQKVLNGYKAGILVLQVQLQSVDPPEQVIAAFKDVTAAQQDQNRLRNEAEAYANRVV
                     PEARGKAAAIVQEAEGYKLQTVAEATGQVSRFNQVYEQYKKAPDVTRERLYLETMERV
                     LGGMNKIILDQSTGQGQGVVPYLPLGPLTPQAGEGGRK"
     gene            2892..3797
                     /locus_tag="VNY06_03590"
     CDS             2892..3797
                     /locus_tag="VNY06_03590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003612487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HWX13941.1"
                     /translation="MKGATTFAAIAAFIVILIVALSSAFTVEQTEQAIVLRFGEPIAG
                     RGLVTTPGLHFKYPFIENVVFLDNRILVVEAPKQEVLASDNNRLDVDSFLRYRIVDPL
                     KFYQTVRSPDLANGQLGFILNSAVRQVLGKANMTEIVRDNRADLMVKIREQVNQEGGN
                     LGIAAVDVRIRRADLPRQISEKVFSRMQSERAREAAEYRAKGSETAQTITATADRDVV
                     VLRGNAQRQADQTRGEGDAERNRIFAAAYGKDPDFFDFYRSMQAYEAGLKGGDTRMII
                     SPKSDFFRYFGTPAGQLAAPAVGHQ"
     gene            3881..4946
                     /locus_tag="VNY06_03595"
                     /pseudo
     CDS             3881..4946
                     /locus_tag="VNY06_03595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019195948.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
BASE COUNT         1114 a         1415 c         1402 g         1015 t
ORIGIN      
        1 cccctcccgg ctcctcgcga ccgcaaaaaa atggcgatat ctcgacactg tcaaggcccg
       61 ctccccgaaa accgtcaaaa gaagacttgc cgtacttcca tcttgcggcg acgattagaa
      121 caaacatcaa tatttagcct cagggcggac aactatggat gagacggtga aaactgaaga
      181 aaatcccgtg acgcatcccc ctcggagtcg caaagacccc ttcgctcagc ccgcctccag
      241 gatcagcccc gctccacccg gtatagcaga cgttatttcg tttaaccggg acgagctgcg
      301 aaagatcttc agcctctatg gccgcaaagt tggcgatgga gagtggcagg attacgcgat
      361 cgattttacg ccgcaaaagg cggtcttttt catttacagg cgggcttgcg aatatgcgct
      421 ttttaggatt gagaaatgcc ctaggctggc acagaagcag ggtgcctatt cggtgatcac
      481 cgcaaccggc cttattctca agcatggtca tgacctcagc gatgttatcg cagcgctcga
      541 cagtggattg aagctcgttt ctgggtgaac agagtccgga gatggcgtct gaatttctta
      601 atcaaccgca actggctagc cgggggctgc cacggaaata gcgtggtcca agacaaggcg
      661 gatttttacc ttttgtagtc acggtgccaa cactatatgg ttgcgagttg atgctcctcc
      721 tcctcggttc acgggggtta caagccattt ctgtctttaa cgcgccttaa ccgctgcgcc
      781 tgccaatatc atagcacctt tcggctatac ttcgctgctt tgggcagctc ttttcagctt
      841 cttgattttt gcagaggttc ctgatctgca cgcaaccatt gcggcaggat tgattgctgg
      901 cgcaggcctg tttatttttc cttggcgaga aaaaacttaa gtgattaggc ttgcagcctc
      961 accgaagcta tatgcaggcg ggaattgcct gtcggccttt cgagcagcaa ttcagccagc
     1021 taatcgcgca agaaattcca gatggaagcg aaccgggagc gcagagccaa tggccaacag
     1081 gcttccaata aatgaatgcg ccgagccctc agacacgggt ctaggtcgct cggatttgcc
     1141 gccaccgctc gccatcgtcg ccgcaatcgg tgaaaatggc gtgattggcg acgggcgcgg
     1201 tctgccgtgg catttgccca gtgacctcaa acacttccgc gagattacca tgggcaagcc
     1261 gatgctcatg ggccggagga ccctagaatc gattggccgc gccctgcccg gccgcgagac
     1321 gatcgtgctt actcacgacc gcgcgtttaa accaccacac agcgtcggcc atgacggcct
     1381 catccacgtg gcgtacgacc ttgagacggc gctcgctctc gctcaagcgc gtgccaagat
     1441 gctgggagcg gaagaagtga tactagctgg cggaggaaat ctttacgaaa gtttgattga
     1501 ccgtgctaaa cgcatgcacc tcaccctggt cgatctcgcg cctaaaggaa gggtgcgttt
     1561 tcccgtaatc gattggtcca attggctgga aacttgccgt gttcggccgc ggccaacggc
     1621 caaggacgag gcaacattcg cttttgtcga ctttgaccgg cgaggttgct tgttattgta
     1681 gtcggaccag tgcacctcta tatggagtgt gaaaccgctt gaagcttggg caattatcct
     1741 tggctttgct ggcgtttcct tgcaaaagag agtcttatgc cctggagcaa tgaaggtgga
     1801 aatggcggtt cctggaaacc cagcagtcca gggccttggg gccaaaagcc gggtgcacca
     1861 ccgcccgaat ttgaagagtt tttaaagcgc gcgcaggaga agctcaaaca atggataccg
     1921 gcgcctggcg gcatgggggg acgtagcctg ctcgccttgg ctctcgttgg cacaatcatc
     1981 tggctcttct ccggatttta cactgtcggc ccgaatgaag tggggctcaa gaaaattttc
     2041 ggccggtata taagcaaaac tgggcctggg ttgaattaca actttccttt cccagtcgga
     2101 agtgtggaaa aacttcaggt caccgatcgc aacaccatca atatcggctt cgccttccgg
     2161 caggacgtgc gcacacccgg gcaaactcag ctcgacctgc cggaagaaag cttgatgctg
     2221 accggcgacg aaaatatcgc cgacgtcaaa tttgtcgtga tctggcagat cgacccagtt
     2281 cgccctgagg attacgcctt caatatctca aaccagcgcg acacggtgaa ggcggtggcc
     2341 gagagcgcca tgcgtgaagt aatcggccgc ggccagattc agcgaattct caccgccgaa
     2401 cgccaaatca tcgagacgga cgtgcaggaa ctcatgcaaa aagtcctcaa tgggtataag
     2461 gccggcattc tggtgttgca ggtgcaattg caatcggtcg acccgcccga acaggtcatc
     2521 gcagccttta aggatgtgac ggcggcgcaa caggatcaaa accgtttgcg caacgaagcc
     2581 gaggcctatg ccaaccgagt cgtccccgag gcccggggaa aggccgcggc gattgttcag
     2641 gaggcagaag gctataagct tcaaaccgtc gctgaggcaa ccggccaagt ttcccgcttc
     2701 aaccaagtct atgaacaata caaaaaagca cccgatgtga cacgagaaag actttacctt
     2761 gagactatgg aacgcgtgct cggcgggatg aacaagatta ttctcgacca gagcaccggc
     2821 caaggccagg gggtcgttcc atatttgccg cttggacctc tgacgccaca ggcaggcgaa
     2881 ggaggccgca aatgaagggc gcaactacct ttgctgccat cgcagcgttt atcgtgatcc
     2941 tgatcgttgc gttgtcctcg gcattcactg tcgagcagac cgaacaagca attgtgttgc
     3001 gtttcggaga gccaatcgcc gggcgcggtc tcgtcaccac gccgggcctc catttcaaat
     3061 accccttcat tgaaaatgtc gtttttctcg acaatcgcat cctggttgtc gaggcgccga
     3121 aacaggaagt actcgcgtcc gacaataacc gtctcgatgt cgattccttc ctgcgctacc
     3181 ggatagtcga tccgctcaaa ttctatcaga cggtccgctc accagacctg gcgaacggtc
     3241 agcttggctt catcttgaac tcggcggtac gccaggtgct ggggaaggcg aatatgaccg
     3301 aaatcgtacg cgacaaccgc gccgatctca tggtcaagat ccgcgagcag gtgaaccagg
     3361 aaggcggcaa cctcggcatc gccgccgtcg atgtgagaat ccgccgcgcc gatctgccaa
     3421 ggcagatttc ggaaaaggtt tttagccgga tgcaaagcga acgcgcacga gaggcggcgg
     3481 aatatcgcgc caagggctcc gaaaccgcgc aaacaatcac agcaacagcc gaccgtgatg
     3541 tcgtcgtatt gcgcggcaac gcccagcgcc aggccgacca gacccgaggc gaaggcgatg
     3601 cggaaagaaa ccggattttc gccgcagcgt acggcaagga ccctgatttc tttgacttct
     3661 accgctcgat gcaagcctac gaagctggat tgaaaggtgg cgacacccgc atgatcatta
     3721 gcccgaagtc ggatttcttt cgctatttcg gcacgcctgc cgggcagcta gccgcgcccg
     3781 ccgtaggcca ccaatagcgc ttcgagccgc cggaccacag atcggttgga aaaacgcgcg
     3841 gcaccgagat ctggcttttg accaaggtca ggatatgaat atggcggagg ggagcgaatt
     3901 tgcgcgttcc ttcgcttggg acgacccttt cgatctcgac gatcagctct ccgaagacga
     3961 gaggctggtg cgcgactccg ctcaagcatt tgcgcaacga tatctggctc cccgcgccgc
     4021 ccaggattat ctggaggagc ggtttgatcg cgagatcatg attgagatgg gccgcgctgg
     4081 actgcttggc gctgtgctta cgctgaaata ttgtggcgcg ggactgagcc attgcttacg
     4141 ggctgccgcc cgcgaggtcg agcgcattga tagcggttat cgttcgctca tgagcgtgca
     4201 ggcttctctc gtgattcagg cgatcgttgc ttacggcagc gatgctcggc gctgcagata
     4261 tttgccaaac ctcgccacgg gggaatggat cggctgcttt ggcctcacgg aaccagatgc
     4321 cggatccgac ccgcaagcca tgcgcaccaa agcggagcaa gccaaaggcg gataccggct
     4381 taccggcacc aagacctgga tcacaaacag cccggtcgcc gacattttca ttgtctgggc
     4441 aaaatcgagc gcggatcaag gcaggatccg gggatttatc cttgaacgcg gaatgcctgg
     4501 tctcacgaca ccgacgattt cgcaaaagct ctcattgcgg gcctcgccaa ccggcatgga
     4561 tggcgttgcg gtgccagaag agaacgtctt gccgagcgca atccggctga aagcgccctt
     4621 tggctgcctc agtcacgccc gcttcggcat ctgttggggg gcgatgagcc cggccgaagc
     4681 ctgtttcaaa gccgcgtgga cctacacgct ggaacggcgc cagtttggca ggccgctcgc
     4741 gtccaaccag ctcattcaaa aaaagcttgc cgatatgcaa accgaaattg tgcttggcat
     4801 tcaggcggcg catcgggcga ctgctggacg caggcaagct cgctcctctc gctcgacaag
     4861 cgcaacaacg ccggcaaggc cctcgatatc gcccgcatgg cgtgcgacat ggcgccggtg
     4921 gccgcagctt tcccgctcat gcgcca
//
LOCUS       DATMPU010000155         2130 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_126979,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000155 DATMPU010000000
VERSION     DATMPU010000155.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 2130)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2130)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2130
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_126979"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..882)
                     /locus_tag="VNY06_03600"
     CDS             complement(<1..882)
                     /locus_tag="VNY06_03600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX13942.1"
                     /translation="MSYIVLALGVLLALGGALALLTSYGIIMVERGWAGVIAGTTALA
                     GGVVTLALGLILHRLSNFYALFTGTTLPPGSAEDETGAPGMAHTPHYVPKAAILPETA
                     PGSAAMPPASGLRSWAQRQARSALKSRGPVAQAALKIRESDSAAPKSPFSPPSLSNGS
                     AAAVDPHSEPGSGPIIPASTAGEAEENTPQHPAPLEETYAEARRNGGRDDPWPRQNNG
                     VSEPLIVPPFPEMPIAADEVHEQSSPRNSWPTEPALIETILPEAGHMPHEPVVEARRE
                     GTEKSLPEAQEEKNLQQP"
     gene            1159..1758
                     /locus_tag="VNY06_03605"
     CDS             1159..1758
                     /locus_tag="VNY06_03605"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019400191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HWX13943.1"
                     /translation="MSFVLPDLPFPYDALKPYLSVETLEYHHDKHHAAYVTTANNLLT
                     DSGLAGKSLEDVVKESHGVNVPLFNNAAQHYNHWEYWKSIKPKGGGKIPGKLQKALIE
                     TFGSIETVKQELVQAGTTQFGSGWAWLAVKDGKISVVKTGNAENPLIQEATPILTIDV
                     WEHTYYIDYRNRRADYLKAFVDNLVNWEYVAERYDAAVK"
     gene            complement(1872..>2130)
                     /locus_tag="VNY06_03610"
     CDS             complement(1872..>2130)
                     /locus_tag="VNY06_03610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HWX13944.1"
                     /translation="GRDGLFSLVKGAPATMKYENSLIMAEGDLLMLHGRFSGIGQPAN
                     WIVVDIVRLEDGRLAEHWDVVKDEATCETSVSGLPMFALGQ"
BASE COUNT          444 a          613 c          608 g          465 t
ORIGIN      
        1 cggttgctgt aaatttttct cttcctgtgc ttcgggcaaa gatttttctg taccttccct
       61 tcttgcttcc acgacgggtt cgtggggcat gtgccctgcc tccggcaaga tcgtctcaat
      121 caaagcaggc tcggtaggcc agctattgcg cggcgaggac tgctcatgga cttcgtcggc
      181 agcaattggc atttcgggga atggcggcac aataagcggc tcgcttacac cattgttttg
      241 gcgcggccat gggtcgtcgc gccccccatt tcgcctggcc tcggcatagg tctcttcgag
      301 cggcgccggg tgttgcggcg tgttctcttc ggcttcgccc gcggttgagg cgggtattat
      361 cggacctgag cccggctccg aatgcggatc taccgccgct gcgcttccgt tcgataagct
      421 tggcggtgag aatggcgatt ttggcgcagc ggagtcggat tcccggattt tcagcgcggc
      481 ttgggcgacc ggcccgcgag acttaagggc ggagcgcgct tggcgctgcg cccagcttcg
      541 caagccggag gctggcggca tggctgctga accaggcgcg gtttcgggca aaatggcggc
      601 tttgggaaca taatgcggag tgtgcgccat gcctggtgcc ccggtctcgt cttcggccga
      661 accagggggt aatgtcgtcc cagtgaacag cgcatagaaa ttggaaagcc gatgcaaaat
      721 aagcccgagc gcaagggtca cgacgccacc cgccagcgcc gttgtccctg cgatgacacc
      781 agcccagccc cgctcgacca ttatgattcc gtaactagtg aggagcgcca aggcaccgcc
      841 tagtgccaga agcaccccta aagcaagcac gatatagctc atcttctcca ctcactggtg
      901 atcgtccggg cctcagacgt cgcagcgaac aagtcgctca tatccgattt ggggcgcgac
      961 attgattcac ccttcggttc ccgccatcac tgttccgtta ccgccaaagg cgctgcctcg
     1021 tcgcactgtg ataagcggca tggctttact cccggaacgg gttgccgttc caaaaggagc
     1081 cgtttacata gggggctgtc ttagccgccg cgactttacg cgtcatctag cggccaattc
     1141 accgacgagg agcccaatat gtcctttgtc ttgcctgatc ttccattccc ctatgatgcg
     1201 ctcaagcctt atctatccgt tgagaccctc gaataccatc acgataagca tcatgcggcc
     1261 tatgttacca cagccaacaa tcttttgacg gatagcggcc ttgcgggcaa gtccctggaa
     1321 gacgtggtca aggaaagcca tggcgtgaac gtgccgctct tcaataatgc ggcgcagcat
     1381 tataaccact gggaatattg gaaatcaatc aagccaaaag gcggcggtaa aattcctggc
     1441 aagctccaaa aggctctgat cgaaactttt ggatcgatag aaacggtaaa gcaggagttg
     1501 gtgcaggccg ggacgacgca attcggttct ggttgggcgt ggctcgccgt gaaggacggg
     1561 aagatctccg tcgtaaagac agggaacgcc gaaaatccct taattcagga ggcaacgccg
     1621 attttgacca tcgatgtttg ggagcatacc tattatatcg attaccggaa tcgcagggct
     1681 gattatctca aggccttcgt cgataatctc gtgaattggg aatatgttgc agaaagatat
     1741 gacgccgcgg tcaaatagca actctgtttc agcgcccgac ggaaggcccg gagtgttttg
     1801 cgtccggaca cgcgacaaga tgacgattcg aatgctctaa tatctgcctc gccggcaaat
     1861 gcggcgatag cttattgtcc caaagcgaac atcggcaggc cgctgaccga cgtttcgcac
     1921 gtagcctcgt ccttgacgac gtcccagtgt tcggcgagcc ggccgtcctc gaggcggaca
     1981 atgtcaacca cgatccagtt cgcgggctgg ccgatgccag aaaaccggcc atgcagcatc
     2041 agcaggtcgc cctccgccat gatcaaactg ttctcatatt tcatcgtggc tggggcgccc
     2101 ttgaccagac tgaagaggcc atcgcgccca
//
LOCUS       DATMPU010000156         3427 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_4464523,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000156 DATMPU010000000
VERSION     DATMPU010000156.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 3427)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3427)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3427
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4464523"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..197)
                     /locus_tag="VNY06_03615"
     CDS             complement(<1..197)
                     /locus_tag="VNY06_03615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012591206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Arm DNA-binding domain-containing protein"
                     /protein_id="HWX13945.1"
                     /translation="MNVRAVAALKKPGRHADGGNLYLKISGAGTSLSKRWTFMYVVDG
                     RQREAGLGSVASVSLAEVRRRA"
     gene            complement(360..1210)
                     /locus_tag="VNY06_03620"
                     /pseudo
     CDS             complement(360..1210)
                     /locus_tag="VNY06_03620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016918524.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
     gene            1552..2214
                     /gene="ftsE"
                     /locus_tag="VNY06_03625"
     CDS             1552..2214
                     /gene="ftsE"
                     /locus_tag="VNY06_03625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018388144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HWX13946.1"
                     /translation="MIRFENVGLRYGMGAEILKDISFSIAPQSFQFLTGPSGAGKTTL
                     LRLILLSLRPTRGVISLFGDDALSLDKAAITNLRRRIGVVFQDFRLLDHLTAYENVAL
                     PLRVRGLDEISYRAEVTELLQWVGLGERMHLLPPILSGGEKQRAAIARALIVRPELLL
                     ADEPTGNVDPTLARRLLRLFTELHKSGTAVVIATHDLGLMDQFDNARRLVLGDGRLHI
                     YD"
     gene            2211..3233
                     /locus_tag="VNY06_03630"
     CDS             2211..3233
                     /locus_tag="VNY06_03630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012383375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWX13947.1"
                     /translation="MRQEFTIEARVARCFAHRGEAAQDRRQVKLHQTVSRGLQRRGRG
                     ERALVPKASIAGRALVTVVAIMTFLASLAAGAAILISDSSRSWQDTVAREMTIQIRPI
                     PGRDLDADMAKAAELARGMPGISSVRAYTKAQSEELLQPWLGSGLDLSELPVPRLIVI
                     ALDREQRLDINVLRKRLAETIPGTSVDDHRLWLERLAVMAKSVVIVAGLIFFLVLAAM
                     ILAVSFATEGAMAGNREIIEVLHFVGAADSFISRQFQRHFFQLGLRGGAIGGVLAILA
                     FFVSQSLASLLRATPGGDQMEAMFGDFSLGWKVYTLIALIAAGIAIVTGLVSRVIVQR
                     HLRDLN"
BASE COUNT          709 a          946 c         1001 g          771 t
ORIGIN      
        1 gccctacggc gcacctcagc gaggctcaca gacgcgaccg aaccgagccc ggcctcgcgc
       61 tgcctgccgt cgaccacgta catgaaggtc cagcgctttg agagcgacgt gccggccccg
      121 gatatcttta gatagaggtt gcccccgtcg gcgtgacggc ctggcttttt cagcgcggca
      181 acggccctaa cattcaatcg gtcgagcgct cgcatgtgct ccagtcctag actttgtact
      241 agccgtgaac ggcgtcttgc atggccatca ataaccacgt atggacaaaa aagcaatgaa
      301 gctctagctt tatcaacatc aaaagaaagt cagcggtgga tagccatacg caccattcga
      361 gcggaaatga gctccgcgat gggcactgac gagacaacgt ggcgggcctt aaaagtttcg
      421 gcggtgccgc tgctgcttat cgcctcgatg gtccaaattg acttttcggg atcaaacagg
      481 aacatctcgc accttcggtc catgacgatg cggccaccaa gctctatgat ttttctggcg
      541 gcggcttccc acatcatgcc agggccgcgc cttggataac gaaacgattc gattagagtt
      601 ttggctccgc catcggtgcg gcgcttgagg cccaatgacc gctgcaaacc gtcggtcagc
      661 gccttgaaaa aattcagccc tttgatcctc tgggccgccc aatcagctga gatctcgtcg
      721 caatccatcc cccaaacctt ttccgtatag gtcttgaaga agatcgaaaa cagacgttca
      781 ccaaattcat ttcgcatcca ttgatgaaat gtcttggggt tttggatcgg gaacgcacgg
      841 gcataagcat aggaggcaag gcaggcagcg ctttgccaaa gccccagatt gcgcagggct
      901 tcgaaagctt ttaggggata ggcgtaaaac ttgccgccat aatagatgcg cgagagtcgc
      961 ggacgggtga gaaatcatcc ggcagaatct cattccaaag ggctacgacc tcgcgtgact
     1021 tcgaaaagaa acggtggcca ccaatgtcaa ataaaaagcc cttatagaag accgtccggc
     1081 taatgccgcc gacgtaatct ttgtcctgtt cgatcacggc cacgtcatga cccgccttgg
     1141 cgagttgata ggccgtggtc agtccggccg gtccggcccc aataattaaa gtatcaatcg
     1201 tctccaccat gcgctccatg ccggcatcgc cgcacggaag agaacaggtg aaggttaagt
     1261 cccggccatc aaaacaaggg caagtttatc gaccacctat gtcgcgctag gtcaggcgcg
     1321 tattttccag ctgccccata tgtatttgtt agtcttaaat tcgcggcagg tggacgaggt
     1381 aatatttatt tcgaggatgt aattcgcgcg cctcgcgtta aattttacgc agaagggtcc
     1441 tgtgggtgcg gcgccagggc ctgcgccagc ttgcgggaaa tttccttgaa tttcccctaa
     1501 aagaccaggg ctcgggaagc gaatcaccgg gcaccgccag cgaggtcgtg agtgatccgt
     1561 tttgaaaatg tcggcttgcg ctatgggatg ggagcggaaa tcttaaaaga tattagcttt
     1621 tccatcgcgc cgcagtcgtt ccagtttttg accggcccct ccggcgctgg caagaccacg
     1681 cttctgcggc ttatcctctt gtccttgcgg ccgactcgcg gggtcatcag cctatttggc
     1741 gacgatgctc tctcgctcga caaagcggcg atcaccaatc tgcggcggcg aattggtgtc
     1801 gtgtttcaag atttccgctt gctggatcat ttgacggcct atgaaaatgt tgctcttccg
     1861 cttcgcgtgc gtgggctcga tgagataagt tatcgcgccg aagtgaccga gctattgcaa
     1921 tgggttggac ttggcgaaag gatgcatttg ctgccgccga tattatccgg cggagaaaag
     1981 cagcgcgcgg ccatagctcg cgcgctcatc gtccggcccg agcttttgct tgccgatgag
     2041 ccaactggca atgtggatcc gacgcttgcc cgcagactgc tgcggctttt cacggaactt
     2101 cacaaatcgg gaacagcagt ggtgatcgcg acgcatgatc tcggcctcat ggaccagttt
     2161 gacaatgcga gacggctggt ccttggtgat ggccgcctgc acatttacga ttgaggcaag
     2221 agtttacgat tgaggcaaga gttgcgcgat gcttcgcaca taggggcgag gcggcacaag
     2281 atcggcggca ggtgaagttg catcaaacag tatctcgcgg gttgcagcga cgcggaaggg
     2341 gcgagagggc gcttgttcca aaagcctcga tcgccggacg agcgctcgtc accgtggtcg
     2401 ctatcatgac ttttcttgcc tcgcttgctg ccggcgcggc aatcctgatc agcgatagct
     2461 cgcgcagctg gcaagacacg gttgcgcgcg aaatgaccat tcagatacgc ccgatcccgg
     2521 ggcgcgatct tgatgccgac atggcaaaag cggccgaact tgcgcgcggg atgccgggaa
     2581 tcagttccgt gcgcgcttac accaaggccc aatcagaaga gctcttgcaa ccttggctcg
     2641 gctctgggct tgatctttcc gagttgccgg tgccccgcct tatcgtcatc gcgctcgacc
     2701 gggaacaaag actggacatc aacgtcctgc gcaaacgact ggcggaaaca attccgggga
     2761 ctagcgttga tgatcaccgc ctgtggctcg agcgcctagc cgtcatggcg aagagcgtcg
     2821 tgatcgtggc tggcttgatc ttctttctgg tgctcgcggc gatgattttg gcggtatcgt
     2881 tcgcgaccga aggcgcgatg gcaggcaatc gcgagatcat cgaggttttg cattttgtcg
     2941 gagctgccga cagctttatt tcgcgtcagt tccaaaggca ttttttccag ctcgggctgc
     3001 gaggtggagc gatcggcggc gtgctcgcga tcctcgcctt cttcgtgtcg caatccttgg
     3061 cgtctttgct cagagcaacg ccgggcggcg atcagatgga agcgatgttt ggggattttt
     3121 ccctcggctg gaaggtctat acattgatcg ctctcatcgc cgcggggatc gcgattgtca
     3181 ccgggctcgt ctcacgagtg attgtgcagc gtcatctgcg cgatttgaat taggcgcatt
     3241 gtgccccgaa ttgttccggg tttgggatgc cgcgggcttg ttgagccaaa agtggaaacc
     3301 ggctcgcgtc tggacaggtt ctcctcttgg cacgtggtga cgaccccgat taggggttgg
     3361 gagcagccag cggcggcggc tcggcgcttc ttctgcgctc cgtaaggctt ggaattcaaa
     3421 cggcgtc
//
LOCUS       DATMPU010000157         2227 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_2928565,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000157 DATMPU010000000
VERSION     DATMPU010000157.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 2227)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2227)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2227
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2928565"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..255)
                     /locus_tag="VNY06_03635"
     CDS             complement(<1..255)
                     /locus_tag="VNY06_03635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020174447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HWX13948.1"
                     /translation="MRFRRYSHSCAQGEGATSSGRLHRHFGLFGGRRRMFDQGDVRLV
                     ILKLISEKPSHGYEIIKAIEDRLGGAYAPSPGIVYPALTLL"
     gene            504..1259
                     /locus_tag="VNY06_03640"
     CDS             504..1259
                     /locus_tag="VNY06_03640"
                     /EC_number="2.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012592011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0018104 - peptidoglycan-protein
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HWX13949.1"
                     /translation="MSFLTRRTIIAGASASAAALAGCVSSNPNPAINAAALSLPLPAS
                     PANSAMADVIEPRYNEIYGEVKDANFTVAAVNLAQVNSAFLRKNIAYETKEQPGTIVI
                     DPANHYLYHVEGGGRATRYGVGVGREGFVWAGEATIKSKQEWPDWYPPKEMMGRRPDL
                     KKSMVELQSGEGMHGGPANPLGARAMYLWQGNKDTYFRIHGTNEPWTIGQSQSSGCIR
                     MINQDAMDLYQKTPIGTRVVVLSSPGAAKVAHV"
     gene            complement(1268..2143)
                     /gene="mutM"
                     /locus_tag="VNY06_03645"
     CDS             complement(1268..2143)
                     /gene="mutM"
                     /locus_tag="VNY06_03645"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011942604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HWX13950.1"
                     /translation="MPELPEVETVRRGLETVLLGARFSRVDQRRPDLRFPFPERFAER
                     LAGRTVEGLWRRAKYLIADLDGADALVMHLGMSGSFRIQQALSIQEPAPEAGIAAKNP
                     AHDHVAFELSTGARVVYNDPRRFGFMLLISRHALAAHPLLQSAGIEPLGDEFDGAALA
                     KLFAGRRTSLKAALLDQKLVAGLGNIYVCEALYRACLSPRRSAITLVRKNGAPSADAL
                     RLARAIREVLEEAIAAGGSSLRDHRKADGGLGYFQHNFGVYGRASEPCIRCGGTIKRI
                     TQSGRATFYCGSCQR"
BASE COUNT          499 a          662 c          632 g          434 t
ORIGIN      
        1 aagcagcgtc agggctggat agacgatgcc ggggctcggc gcataagcac cccccagacg
       61 gtcctcgatc gctttgatga tttcatagcc gtgcgagggc ttttcgctga tcagtttcaa
      121 aatgacgagg cggacatcgc cctggtcgaa catccggcgg cggccgccga agagaccgaa
      181 atgccggtgc aagcggccgc tgctggtggc accttcgcct tgcgcgcaag aatgggagta
      241 acggcggaaa cgcatgggaa actcccgcag tgctaatcac gatatatcta agttagatat
      301 atctagatag acttccgcaa ggcccgtccg aaaaatattt tcctgttcaa agggacgcga
      361 cgggtttccc cgaggcggcg gaaacccatt ggcaattccc gccaaagaaa acccgcgagc
      421 gtgacatttc cgtcatatcg tggcgcgttt ttatgggtta caaagtagaa atctcgccaa
      481 cccatttttg attcggtcct cacatgtctt ttctcacccg ccgcacaatc atcgccggag
      541 cgtccgcgtc agccgccgcc ctcgccgggt gcgtgagctc gaaccccaat cctgcaatta
      601 atgccgccgc gctttcgctc ccgctgccag cttcgcctgc aaattccgcc atggcagatg
      661 tgattgagcc gcgctacaat gaaatttacg gggaagtcaa agacgccaac ttcaccgttg
      721 ccgccgtcaa tttggcgcag gtcaattcgg catttttgcg caagaacatc gcctacgaga
      781 ccaaggaaca gccgggcacg atcgtgatcg atcccgccaa tcactatctc taccatgtgg
      841 aaggcggcgg cagggccacc cgctatggcg tcggtgtcgg acgcgaaggc ttcgtctggg
      901 ccggggaagc aacgatcaag agcaagcagg aatggccgga ctggtatcca cccaaggaaa
      961 tgatgggccg caggccggac ctcaagaaat cgatggtcga gctgcaaagc ggggagggca
     1021 tgcacggcgg ccccgccaac ccgctcggcg cccgcgccat gtatctttgg caagggaaca
     1081 aggatacata ttttcgcatc catggcacca atgagccttg gacgattggg cagagccaat
     1141 cctcgggctg catacgcatg atcaaccagg atgcgatgga tctttatcag aaaacgccga
     1201 tcggcacccg ggtcgtggtg cttagctcgc cgggagcagc caaagtcgcg cacgtttgag
     1261 tatcctgtta tctctgacag ctgccgcaat aaaaggtggc gcgacctgac tgcgtaattc
     1321 gctttatcgt gccaccacac cggatgcagg gttcactggc gcggccataa acgccaaaat
     1381 tatgctggaa ataaccgagc ccaccgtcgg ctttgcggtg gtcgcgcaag gatgagccgc
     1441 ccgccgctat cgcttcctcg agcacttccc gaatggcacg cgccaggcgc aaagcatccg
     1501 cgctcggcgc accattcttc cggacgaggg tgatggcgga acggcgcggc gacagacagg
     1561 ccctgtacaa agcctcgcac acataaatat ttccgagccc ggcaacgagc ttttggtcga
     1621 ggagcgcggc tttgagcgat gtccgccttc cggcaaaaag tttggcaagc gcggcaccat
     1681 caaattcgtc gccgaggggt tcaattccgg cgctctgcaa cagtggatgc gcggcgagcg
     1741 cgtggcgtga aatcaatagc ataaagccaa agcggcgggg atcattgtag acgacacgcg
     1801 cgcctgtcga aagctcgaac gcgacatgat catgcgcggg gttttttgca gcaattccag
     1861 cctcgggcgc gggctcctgt atgctcagag cctgttggat acgaaacgat ccggacatgc
     1921 caagatgcat gacaagagca tcagcgccgt cgagatcagc aatgagatac tttgcccgcc
     1981 tccaaagtcc ctcgaccgtt ctgccggcga gcctttcggc aaaacgttct ggaaaaggaa
     2041 acctcagatc cggtcggcgc tggtcgaccc tgctaaaccg cgcccccaac aacactgttt
     2101 caagcccgcg gcggacagtt tcgacttcgg gcagctccgg cactttctgg tccttattta
     2161 agttgcgcaa cctttggaca agcctatcgt ccgtccattc actgcttatc ggattttgac
     2221 gctgggc
//
LOCUS       DATMPU010000158         2537 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_3233032,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000158 DATMPU010000000
VERSION     DATMPU010000158.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 2537)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2537)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2537
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3233032"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1967
                     /locus_tag="VNY06_03650"
     CDS             <1..1967
                     /locus_tag="VNY06_03650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012383553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HWX13951.1"
                     /translation="AQMTGSSAKAAVAGPRSVALIGPYGCGKSTLFDALMAAAGAAVR
                     RTGDSRKHAASTEVRLGHCSFLGDRWSIVDCPGSVEFAYEAEAALAAVDFAIVVCEPS
                     ATKISSVPLLFKLLEKFQLPHLVFVNKIDTLTGSFDELLTALQAHSKFPLAPRQFPIR
                     EGEAVIGYIDVVRGQAYRYRTNQSAEAMELPADLREPEKQAFDGLTEVLADQDDALLE
                     KLIEEVAPTLAEIYQNLHKDQASGTIVEVLLGSAAQGQGIFPLWKTLRHDVPDSIETA
                     ARRGIAASGEPLVQIFKTVQAGKLSYGRIWRGPLNDGATFDGVRVGGLHRFSGSEQIR
                     VQEADAGELVALGRLDSVATGAVLGTPAATESLTFPTSPPPVFALAIMAKDRKDDVKL
                     PGALQKLVSEDPALGVVHDQETGETLLTGQGDIHLNTALERLATNYNLQIATSAPKIA
                     FKETIKRKVLQHSRLKRQTGGHGQFADVKLEIEPRHRGEGFLFIDKIVGGAVPRQYIP
                     AVREAAEEAMQKGPFGHPVVDVAVTLVDGTFHSVDSSDMAFRTATRLGISEGLAKAEP
                     VLLEPIEHVTVSVPNTSTAAAQRLLTGRRGQILGYAERPGWPGWDDVEALVPAAELHD
                     LITQLRSETQGLGHYRRRFDHLAEARGMTP"
     gene            complement(2217..>2537)
                     /locus_tag="VNY06_03655"
     CDS             complement(2217..>2537)
                     /locus_tag="VNY06_03655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX13952.1"
                     /translation="LHPVLLEQHGARLGPDKLPAASRHGTKAYTRINGTDLRLNHIPT
                     KVLFSNDPISMPPAVKRNRPPCCEPRRKFSIVRAGTLLPSAAAAPICAPLRLQSEAAA
                     ALLG"
BASE COUNT          516 a          720 c          782 g          519 t
ORIGIN      
        1 gcgcccaaat gacaggttct tctgcaaagg ctgccgtggc gggaccccgt tcggtcgcat
       61 tgattgggcc ctatggatgc ggcaaatcca ctctttttga tgcgctgatg gccgcggcgg
      121 gggcggccgt aaggcggacc ggggactccc gcaaacacgc cgcgagtacc gaggtgaggc
      181 tcggccattg ttcttttctt ggtgatcgct ggtcgatcgt ggattgcccc ggctcggtgg
      241 aatttgccta tgaggctgag gcagcgctcg ccgcggtcga ttttgccatc gtcgtttgcg
      301 agccctcagc cacaaaaatt tcgagcgtgc ctctgctctt caagttgctt gagaagtttc
      361 agcttccgca cctcgtcttt gtcaacaaga tcgacacgct gaccggttct tttgatgagc
      421 tgctcacggc gctccaagcg cattccaaat ttccattagc gccgcggcag ttcccaatcc
      481 gcgagggaga agcggtgata ggctatatcg acgtcgtgcg aggccaggcg taccgctacc
      541 gcacaaacca aagcgcagag gcaatggagc ttccggccga tttgcgcgaa cctgaaaaac
      601 aagcttttga cggactgacg gaggtgctcg ccgaccagga cgatgccctt ttggaaaagc
      661 tcattgaaga agtcgcgccc acattggcgg agatctacca gaatttacat aaggaccagg
      721 ctagcggcac gatcgttgag gttctgctcg ggtcggcggc gcaagggcag ggcatttttc
      781 cactttggaa gacgctgcgg cacgacgtgc cggactcaat cgaaaccgcc gcccgcaggg
      841 gcatagcagc gagcggagag ccactcgttc agatttttaa gaccgtccag gcgggcaagc
      901 tttcctatgg ccgtatctgg cgcggccctt tgaacgacgg cgcgacattc gatggcgtgc
      961 gtgtcggggg cctccatcgt ttctctggca gcgaacagat cagggtacag gaagccgatg
     1021 ctggggaact ggttgcgctg ggcaggctgg attcggttgc aactggtgcg gttctcggaa
     1081 cacctgcagc aaccgagtca ttgacctttc caacctctcc gccgccggtc ttcgcgctcg
     1141 cgatcatggc gaaagaccgc aaagacgatg tgaagttacc aggtgcgttg caaaaactcg
     1201 tctcagaaga cccggcgctt ggtgtcgtgc acgaccaaga aaccggcgag actctgttga
     1261 cagggcaggg cgacattcac ctcaacaccg ccctcgaacg gctcgccaca aattataact
     1321 tgcagatcgc gacatctgcg ccgaaaatcg cattcaagga aacgatcaaa cgcaaggtgc
     1381 tccagcactc ccgtctcaag cgccaaaccg gcggccatgg ccaattcgcc gatgtaaagc
     1441 tcgaaattga gccgcgccac cgcggcgagg gttttttgtt catcgataag atcgttggcg
     1501 gcgcggtgcc aaggcaatat attccggcgg ttcgcgaagc tgccgaggag gccatgcaaa
     1561 aagggccatt tggccaccct gtcgtcgatg tcgcagtcac tttggtcgat ggcacgttcc
     1621 attccgtcga tagctccgac atggctttca gaaccgcgac ccgattaggt atttccgaag
     1681 gtctcgccaa agcggaacct gttttgcttg agccgattga gcatgtcacg gtgagcgtgc
     1741 caaatacttc taccgccgcg gcccaacgct tgctgaccgg acgccgcggc cagattctgg
     1801 gttacgccga gcggcccggc tggcccggct gggatgatgt cgaggcgctt gtgccagcgg
     1861 ccgagctgca cgatctcatt actcagctgc ggtcggagac ccaggggctt ggccattacc
     1921 gccggcgctt cgatcatctc gccgaagcgc gcggcatgac accttagctg ctattcacct
     1981 cagcgtgagc atagatacgg aagcagatat tttgcgcacg ggatcagacg cgcgcgcaaa
     2041 atcattgcga agtcttatcg cgcgcgcccg ttacttggcg actctcgaca gcgtggccag
     2101 cttaaaccct tgtgatcgag tattgcgagg gagcaattcg tgcgggcgag cgaagacgag
     2161 gctcatgaat cggcgccgca ggtgcctttg cggtgcttgc tcaccatcac ggacgcttac
     2221 ccaagaagcg ccgccgcagc ttccgattga aggcgaagag gagcgcatat tggcgctgct
     2281 gccgcgcttg gcaagagcgt tccggcgcga acgatcgaga acttgcgccg cggttcgcag
     2341 cagggaggac gattgcgctt taccgccggt ggcatgctga tcggatcgtt gctgaaaaga
     2401 actttggtgg ggatatggtt gaggcgaaga tccgtgccgt tgattcgagt gtaagctttc
     2461 gtgccgtggc gggaagctgc gggaagcttg tcagggccga gccgtgcgcc gtgttgttcg
     2521 agcaagacag gatgaag
//
LOCUS       DATMPU010000159         2991 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_1379805,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000159 DATMPU010000000
VERSION     DATMPU010000159.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 2991)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2991)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2991
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1379805"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..496)
                     /locus_tag="VNY06_03660"
     CDS             complement(<1..496)
                     /locus_tag="VNY06_03660"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate binding protein"
                     /protein_id="HWX13953.1"
                     /translation="MRRRDFLGVLGGAAATWSLAARAQQTRRRVGALILGNADAQSFE
                     ADLREELRKRGYVEGQNIQYEFLSADGNIALLSKLAAELVALKVDIIVALYTPCALAA
                     KQATRDIPIVAISGDLVAFGLVASLARPGGNITGISMIGAELFGKSFELLREALPGLR
                     RVAAL"
     gene            complement(833..1216)
                     /locus_tag="VNY06_03665"
     CDS             complement(833..1216)
                     /locus_tag="VNY06_03665"
                     /inference="COORDINATES: protein motif:HMM:NF018717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1330 domain-containing protein"
                     /protein_id="HWX13954.1"
                     /translation="MKTRYTVALAMLTGVAIGAAAVQGLHAQAKPKAYQVTELEVIDD
                     AANKVFAPLVAASQKAAGGRNLQTNGGKIVAFVGDAPKRVSITEWDSLDQAVAWRNGN
                     GWKDLSPQRDKAIKILRQYFVEATN"
     gene            complement(1491..2477)
                     /locus_tag="VNY06_03670"
     CDS             complement(1491..2477)
                     /locus_tag="VNY06_03670"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWX13955.1"
                     /translation="MRRREVIGLLGGAAVAFPWPALGQKDLPLVAVLVPGTAKLANDR
                     IVAIRKGMQEAGLAEGVDYTFALRYADGVFDRLPGLALEFAALKPRVIVASAAAAVAA
                     HKNLPNLPLVFTSYAADPIKAGFAESYLHPGGHATGNVMNAVGGEEALTQKRMNFFKE
                     LVPDLKRLGFIGSSSILATAELNALQGIAGQFGFEIVRHPAQGLDDIEDTVAASVRDG
                     VGALYVSGEPLLYNNMSRVLPLVMAAGKPTVGTYPEWGRAGLLMSYSADLIDDFRRAG
                     IYAAKILGGEKPADLPIEQASKFIFVINLKTAKALGITVPPILFSLTNEVIE"
     gene            complement(2474..>2991)
                     /locus_tag="VNY06_03675"
     CDS             complement(2474..>2991)
                     /locus_tag="VNY06_03675"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWX13956.1"
                     /translation="AEQGNRVSRIALLRDPTNPSHLLLDDRILAAARALELKPQLIDV
                     RGAADFQGAFAAILEQRAQALFVYPLPLAPAQIGQIVEFALNNKLPGMTFWEGYTEQG
                     FLMFYGARNSDQYRRAVGYVDKILKGAEPASLPVEQPTKFELVINVKTAKTLGLEIPS
                     SLLARADEVIE"
BASE COUNT          630 a          905 c          873 g          583 t
ORIGIN      
        1 caagagccgc cacgcggcgc aacccaggaa gcgcctcgcg taacaattca aaagatttgc
       61 cgaacagctc cgctccaatc atagagatgc cggtgatatt cccgcccggt cgagctaaac
      121 tcgcgaccaa gccaaatgca accaggtcac ctgagatagc gacgatcgga atgtcgcgcg
      181 tcgcctgctt cgctgccagc gcgcaaggcg tgtagagcgc tacaatgata tcgaccttga
      241 gcgcgaccag ttccgccgca agtttggaca ggagggcaat attgccgtcc gctgaaagga
      301 actcgtattg gatgttctgc ccttcgacat aacccctctt gcgcaactct tcccgcaggt
      361 ctgcctcaaa ggactgagca tccgcattgc ccaggatgag cgcgccgacc cgccgccttg
      421 tctgttgcgc gcgcgcggcg agtgaccacg tcgccgccgc tccacccaga acaccgagga
      481 aatcccggcg ccgcatgtcc ccccctaggc tcaggagcaa gcatcgtagc agttcaaacg
      541 agtgctttga caggggctga atccggcttc gtcaccgcaa catgaaatgg cggtcgatgt
      601 cgcagatggg tcaagaacgg taaggctcaa agcgagcaaa tctcgtccgg tttaccctcg
      661 ataacggaca tgaacgggcc gcgacgccac ttccgaaaag tgccaaaacc tgactcatgc
      721 gccgcaccaa gcgatgatgt agaggcgcgt cctgttagac tcggatgtta aaaccgcccg
      781 caaaggcaaa gaggccgcca actgaggcgg cctcggtaag cggtaaacaa ctctagttcg
      841 ttgcttcgac gaagtactgc cgtaatatct taattgcctt gtctcgctgc ggcgagaggt
      901 ccttccagcc gttcccattg cgccatgcca ccgcctggtc caggctgtcc cactcggtaa
      961 tggagacacg cttgggcgcg tcgcccacga atgcaacaat ctttccaccg ttggtctgca
     1021 aattgcggcc gccggccgcc ttctgcgaag ccgcaacgag cggcgcaaag actttattgg
     1081 ctgcgtcatc gatcacttca agctcggtga cttggtaggc ttttggcttg gcttgcgcat
     1141 ggaggccttg gactgcggca gcgccaattg cgacgccagt gagcattgcc aacgcgaccg
     1201 tatatcgagt tttcatgact tcctcccaat tgccgaaaag ccggcagtca ataatagcgc
     1261 aacccccacg acggcaatat gatcttcggc cactgccatt tttagtttca agttcggcct
     1321 aggtgcgcgt taattgcgtg actggccgag gtcagctttg aggtctatct cggcccttcg
     1381 tcgctatccg ctgaacgtcc gggttgcctc gataacggac attgtacgga catgcccgga
     1441 ggtcggagtc gtgccaaaag ctgccgtgag caggtgcagc aaagagggca ctattcgatc
     1501 acctcgttag tgagcgagaa taatatcggg ggaaccgtga tgccgagcgc cttcgcagtc
     1561 tttagattga tgacaaaaat aaacttgctt gcctgctcga tcggaagatc cgccggcttc
     1621 tcgccgccga gaatcttcgc ggcatagatg ccggcgcggc ggaagtcgtc gatcagatcg
     1681 gccgaatagg acatcaggag cccggcccgt ccccattcgg gataggtgcc gaccgtcggc
     1741 ttccccgctg ccatgaccag cggcaatacg cgcgacatat tgttgtagag cagcggctcg
     1801 cccgagacgt agagggcgcc aacgccatcg cggacgctcg ctgccaccgt gtcttcaatg
     1861 tcgtctagac cttgggccgg atgacgcacg atctcgaagc cgaattgacc agcgatacct
     1921 tgcaatgcgt tgagttcagc agtcgccaga atcgacgatg aaccgatgaa gccgagccgc
     1981 tttaggtccg gcaccagctc cttgaaaaaa ttcatccgct tttgtgtgag tgcttcttcg
     2041 ccgccaaccg cgttcatcac gtttccggtc gcgtggccgc ccgggtggag gtagctttcc
     2101 gcaaatcctg ccttgatcgg gtcggcagca tacgaggtga agaccagcgg taaattcggc
     2161 aagttcttgt gagcggcgac ggcagccgcc gccgaagcga cgataacgcg cggcttcaat
     2221 gcagcgaatt caagggcaag ccccggcaat ctgtcgaaga caccgtcggc atagcgcagc
     2281 gcaaaagtgt agtcgacgcc ctcggcgagg ccagcctcct gcatgccctt gcggattgcg
     2341 acgatcctgt cgtttgcaag cttcgcggtt cctggcacca gcaccgcgac aagcggcaag
     2401 tctttttgac cgagtgcggg ccaagggaag gcgacggccg caccgccgag caggccgatg
     2461 acctcgcgcc gcctcattcg atgacctcgt cggcgcgagc gagcaatgag gatgggattt
     2521 cgaggccgag cgtcttggcg gtcttgacgt tgattaccag ctcgaacttc gtgggctgct
     2581 ccacgggcag gctagcgggc tcggcgccct tgaggatctt gtcgacgtag cccacggcgc
     2641 gacggtactg atccgagttc cgcgcaccgt agaacatgag gaagccctgc tcggtatatc
     2701 cttcccagaa ggtcatccct ggcagcttgt tgttcaacgc gaattccaca atctggccaa
     2761 tctgggcggg cgcgagcggt aacggataca cgaaaagcgc ttgcgcgcgc tgctcaagaa
     2821 tcgcggcgaa cgcgccctga aaatccgccg cgccccgcac gtcaataagc tgtggcttca
     2881 gctccagcgc gcgggccgcc gctaagattc ggtcgtcgag gagtaggtga gaaggattag
     2941 tcggatcccg gaggagggct atgcgagaga cgcgatttcc ttgctcagct g
//
LOCUS       DATMPU010000160         4541 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_436826,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000160 DATMPU010000000
VERSION     DATMPU010000160.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 4541)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4541)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4541
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_436826"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(57..1895)
                     /gene="sdhA"
                     /locus_tag="VNY06_03680"
     CDS             complement(57..1895)
                     /gene="sdhA"
                     /locus_tag="VNY06_03680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012385672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HWX13957.1"
                     /translation="MTTSTLDNNIAAPPFNGAAYPITDHTFDVVIVGAGGAGLRATVG
                     CSQAGLRTACITKVFPTRSHTVAAQGGVAASLGNMGPDNWKWHMYDTVKGSDWLGDQD
                     SIEYLCRNAPEAVYELEHWGVPFSRTEDGRIYQRPFGGMTTDFGKGPPAQRTCAAADR
                     TGHAMLHTLYGQALRFKTEFFIEFFAIDLIMEDGRCRGVVCLKLDDGTIHRFRAQIVM
                     LATGGYGRTYFSCTSAHTCTGDGNAMVLRAGLPLQDMEFVQFHPTGIYGAGCLITEGS
                     RGEGGYLTNSEGERFMERYAPSVKDLAPRDIVARAMTIEIREGRGVGKYNDHIYLHLE
                     HLDPKILHERLPGISESAKIFAGVDVTREPIPVLPTAHYNMGGIPTNFHGEAVTKKGG
                     DPDASVPGLMAIGEAACVSVHGANRLGSNSLIDLVVFGRAAGLRAAELVTPGAKQAEL
                     PADSAGQALSRLDRFRYAKGEIPTAELRLRMQKVMQNHCSVYRTGTILEEGSQMIHEV
                     WAARDQISVTDRSLIWNSDLIETLEFDNLILQAVVTMKSAEQRTESRGAHAREDFSAR
                     DDINWMKHTLASIDADRRMVSIDYRPVHSFTMTNEMHYIEPKKRVY"
     gene            complement(1978..2370)
                     /gene="sdhD"
                     /locus_tag="VNY06_03685"
     CDS             complement(1978..2370)
                     /gene="sdhD"
                     /locus_tag="VNY06_03685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012592522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045282 - plasma membrane succinate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, hydrophobic membrane
                     anchor protein"
                     /protein_id="HWX13958.1"
                     /translation="MAKGSASVGTARNRVRFLGSARSGTADAWAIRVTSVMLVPLSIA
                     FVSVVLSLLGKDHAAVRAELGHPIPAILMLFFILATVFHMKLGMQSIIDDYVHDMHLK
                     EWSLIANLVFSISIGLACVYAMLKLSFA"
     gene            complement(2444..2857)
                     /gene="sdhC"
                     /locus_tag="VNY06_03690"
     CDS             complement(2444..2857)
                     /gene="sdhC"
                     /locus_tag="VNY06_03690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006724455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045282 - plasma membrane succinate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, cytochrome b556
                     subunit"
                     /protein_id="HWX13959.1"
                     /translation="MARVETDVSGVEKRRPLSPHLQIYRPMLTMVMSIAHRITGAALY
                     FGTLLLAWFLIAAAIGGSTFELAANCFNSIPGRVILFGFTWALIHHFLGGIRHIIWDT
                     GHGFEPKEREWLAQATLYGGIALTAAVWVIAYLIQ"
     gene            3295..4158
                     /locus_tag="VNY06_03695"
     CDS             3295..4158
                     /locus_tag="VNY06_03695"
                     /inference="COORDINATES: protein motif:HMM:NF014305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase"
                     /protein_id="HWX13960.1"
                     /translation="MFGIACLPSHAVAAAGRFTISSGGSPRAAILIQHNRLKKGRRPA
                     IIVLRSARKQGAHLHRSFSLEEMARSSGAVLVYPEPLSGHWADVSGPEANWDTHFVRD
                     LIAKLVSQGIANPSKVFLVGIGDGGIVALRLACDGKQKFAGVAILASSLPSGLEASCA
                     PPTPIPLLMIAGTSDPQVPYHDGRDRLPHDKSELLSVDKTLGVFGKAAGCAGGLTTTA
                     FPDKDIRDGTRAYRDKLNNCAVPVEAVRIEAGGLTLPGVRGEAGAGVGLTNGDVNSAK
                     LVWDFFRPFGG"
BASE COUNT          928 a         1318 c         1343 g          952 t
ORIGIN      
        1 ttggacgcgt gagttggcgg ggagtgagaa ttggaccatc tgtttgtcag cctttgtcaa
       61 tacacgcgct ttttgggctc aatatagtgc atttcattgg tcatcgtgaa actatgcact
      121 gggcggtaat caatcgagac catgcgccta tcggcatcga tcgaggccaa agtatgcttc
      181 atccaattga tatcgtcgcg cgcggaaaaa tcttcccgcg catgcgcgcc gcgggattcg
      241 gtacgctgct ctgcgctctt catggtgacg accgcttgaa ggatgagatt atcaaattcc
      301 aaagtctcga tgaggtcgga attccaaatc aacgagcggt ctgttacgga gatctggtct
      361 ctagcggccc agacctcgtg gatcatctgc gagccttcct ccaaaatggt tccggtgcga
      421 tagaccgagc aatggttctg catcactttc tgcatcctga gacgcagctc ggcggtcggt
      481 atttcgccct tggcatagcg gaaacggtcg aggcgggaca atgcttggcc ggcggaatcg
      541 gctggaagct ccgcctgttt ggcgcccgga gtcacgagtt ccgccgcgcg aaggccagcg
      601 gcgcggccga agacgacgag atcgatgagc gaattcgagc cgagcctgtt ggcgccatgg
      661 acggagacgc aggcggcttc gccaattgcc atcagcccgg gcacgctagc gtccggatcg
      721 ccccccttct tggtgacggc ttcgccatga aaattcgttg gaatgccgcc catattgtaa
      781 tgcgctgtgg gcagcaccgg gatcggctcc ctggtcacat cgacgcccgc gaaaattttg
      841 gcgctttcgg agatgccggg caagcgttcg tgcaggattt tcgggtcgag atgttcgaga
      901 tgtaaataaa tgtggtcgtt gtacttgccg acgccgcgtc cctcgcggat ttcgatggtc
      961 atcgcgcgcg ccactatatc gcgcggcgcg aggtctttta ccgaaggcgc atagcgctcc
     1021 ataaagcgct cgccctcgga attggtgagg taaccgcctt cgccgcgcga gccttccgta
     1081 ataaggcaac cggcgccgta aatgccggtg gggtgaaatt gcacgaattc catgtcctgc
     1141 aagggaagcc ccgcgcgcag caccatggca ttgccgtcgc cggtgcaggt gtgcgccgag
     1201 gtgcaggaga agtaggtccg tccgtagccg ccggttgcca gcatgacaat ctgggcccga
     1261 aagcggtgaa ttgttccgtc gtcgagtttc aggcagacaa cgccgcggca gcggccgtct
     1321 tccatgatga gatcaatagc gaagaactcg atgaagaatt ccgttttgaa acgcaaagcc
     1381 tgaccataaa gcgtgtgcag catcgcatgg ccagtgcggt cagctgccgc gcaagtgcgc
     1441 tgcgcgggcg gtcccttgcc aaagtcggtc gtcatcccgc cgaaagggcg ctggtaaatg
     1501 cggccgtcct cggtgcgtga gaacggaacg ccccaatgtt cgagttcata gaccgcctct
     1561 ggcgcattcc ggcaaagata ttcaatcgaa tcctgatcgc cgagccaatc cgaccccttg
     1621 acggtgtcgt acatatgcca cttccaattg tccggcccca tattgccgag cgaggcggcg
     1681 acaccccctt gtgccgcgac tgtgtgggag cgagttggaa aaaccttggt gatgcaggcc
     1741 gttcgcagcc ccgcttgcga acagccgacg gtcgcccgca atcccgcgcc gccggcgccg
     1801 acgataacca catcgaacgt gtgatcggtt atcggatagg ccgcgccatt aaaaggggga
     1861 gcggcaatgt tgttgtcgag cgtggaggtg gtcatgctat cgtctttcga ggaaatcgta
     1921 aggcccttag cgccgccgcg cgaaggctga tccaaaaccc tgtccgttgg ctacccctca
     1981 agcgaagctc aatttgagca ttgcatagac acaagccaag ccgatgctaa tagaaaagac
     2041 aagattggcg atcagcgacc actccttaag atgcatatca tgcacgtaat cgtcgataat
     2101 ggactgcatg ccgagcttca tgtgaaatac agttgccaaa ataaagaaga gcataaggat
     2161 tgccgggatc gggtggccaa gttcggcgcg caccgccgcg tggtccttgc caaggagcga
     2221 caacacgacc gacacaaagg caatcgagag cggcacgagc atgaccgatg tgacgcgaat
     2281 agcccatgcg tccgccgtgc cggacctggc agagcctagg aagcgcaccc tattcctggc
     2341 tgtgccaacc gacgcggaac ccttcgccat gattgcctac ccttccgata gattgtcgca
     2401 taccgctgtc ataaatccgc aattggctgc cgccgaaaaa gcgctactga atgaggtaag
     2461 caattaccca gactgcggcc gtcaaggcaa taccgccata taacgttgct tgcgccagcc
     2521 actcgcgctc tttaggctcg aacccgtgac ccgtatccca tatgatgtga cgaatgccac
     2581 cgagaaaatg atggatgagg gcccaggtaa agccgaatag tatcactctg ccgggaatcg
     2641 aattaaagca attggcggcc aattcgaaag tgctgcctcc gatggcagcc gcaatcagga
     2701 accacgccag taacagtgtt ccgaagtaaa gcgccgcccc agtgatgcga tgggcaatgg
     2761 acatcaccat ggtcagcatc ggccggtaga tctgcagatg cggggagagg ggtcgccgtt
     2821 tttcgactcc gctcacatcg gtctccactc ttgccatgac atctttttcc ccgcgccaat
     2881 tcgctgttcc ctccagtaat gcaattgttt cgcttgggca acgcgggcag tttgaaatga
     2941 cggactcgtc caaatccaaa cgaaaagcat agggggaaaa gcgccgccag attgttagat
     3001 tgctcgtttg gttgggtcca atttgctgcg gcgcggcgaa agctcctgat gggcttgcgc
     3061 aggggcagct tgccgggcgg ccggaaaaac cgatcgcgtg cggcgatggt tgagcatgcc
     3121 acttgaagcc aggccccaaa tttgcattgt agtttggatc gtatccttct tccaaaggca
     3181 gacgggcaag tcggaccttg caatggctgg catatttccg tggtgaagtt gacagcacgc
     3241 agacgtacat tggctgccct tgttgccgga tgtttggcgg gggctgcagg gattatgttt
     3301 ggcatcgctt gcctgccatc ccatgcggtc gccgcggcgg gccgtttcac gatcagttcg
     3361 ggaggcagtc cgcgcgccgc aattctcatc caacataaca ggctcaagaa gggacgccgg
     3421 ccggcaatca ttgttcttcg ttctgcaagg aagcagggtg cgcatctgca ccggagtttc
     3481 agcctggagg aaatggcgcg gtcgtccggc gcagttctcg tttatccaga accgctatct
     3541 ggccattggg cggatgtttc tgggccggaa gcgaactggg atacccactt tgtgcgcgat
     3601 cttatcgcga aactcgtgag ccaaggtatc gccaatccga gcaaggtttt tctcgtcggc
     3661 ataggtgatg gcggaatcgt ggctttgcgg ctcgcatgcg acgggaagca aaaattcgcg
     3721 ggagtggcca ttcttgcttc tagcctgcct tccggtctcg aagcatcatg tgcaccgcct
     3781 accccaattc cgcttttgat gattgccggc acctcggatc ctcaggttcc ttatcatgac
     3841 gggagggaca gattgccgca tgacaagtcc gagctgcttt ccgtcgataa gacactcggg
     3901 gttttcggca aggcagcggg ctgcgccgga ggtctgacga ccacagcttt tcccgacaag
     3961 gatatccgcg acggtacgcg cgcctatcgc gacaaactga acaactgcgc tgtgcctgtc
     4021 gaggcggtcc ggatcgaagc gggtggtctc actcttcccg gcgtccgggg cgaggccgga
     4081 gccggggttg gccttacaaa cggcgacgtc aacagcgcga agcttgtctg ggatttcttc
     4141 cgtccgttcg gcggctgatc ccgtctgcct ccgtccccgc tcccgccgca gtctcgcggc
     4201 gggctacatc gcgcgactgg tcacgtgttc tcgcttaatg ccctcagtcc ttccttcaag
     4261 gttatggcgg cggcgccctt gtccgccttc ccaacgcctc gaccagttgc aatctcggcg
     4321 gggccggctt gcctcgcgag tgagggctgc gcgtcgccgc atttagtacc cccggctcag
     4381 tgggcaggag ctcgctgtct ctatctcgcc gctctcgctg aagccttgcg cacttgcgcc
     4441 aggcaaaaag ctgcgaggcc ctgagccggt atctgcgcgc aacggagcac accgactggc
     4501 cgctctcaac actttcagcg acgatccgcg ccttctcctc g
//
LOCUS       DATMPU010000161         4902 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_1998048,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000161 DATMPU010000000
VERSION     DATMPU010000161.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 4902)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4902)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4902
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1998048"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            28..609
                     /locus_tag="VNY06_03700"
     CDS             28..609
                     /locus_tag="VNY06_03700"
                     /inference="COORDINATES: protein motif:HMM:NF014470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HWX13961.1"
                     /translation="MFEFDAGKLIIIAIVALIVIGPKELPRVMRHVGAALAKMQRMAA
                     EFRGQFLDAMRESEIDDIKSEVKTLTDSSKTDFGIDPLAQIKAELTQALEAAEKSTSP
                     PETGAVALVSAQNGEMAATHSVSPCTPDGSEMAGLSVAKPGDAPAAPEDKTAAAGAID
                     AEMRALADAIAAKIGEAAPGGAADEHVKAQDNR"
     gene            673..1449
                     /gene="tatC"
                     /locus_tag="VNY06_03705"
     CDS             673..1449
                     /gene="tatC"
                     /locus_tag="VNY06_03705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020176178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HWX13962.1"
                     /translation="MTEADIEATKAPLMDHLIELRARLIKALVAFLVMFIVSFYFAKD
                     IYNILVVPFEHAAGPDARLIYTAPQEYFFTQIRVALFSAAFLSCPVVFAQLYAFVAPG
                     LYKHERKAFAPYLMATPLFFAAGALLVYFVVMPNLLLFFIGMQQAKEPGRAQIELLPR
                     VSEYLSLIMTLIFAFGITFQLPVVLTLLGRVGIVTSDFLRRQRRYAVVLVFVLAAILT
                     PPDIFSMLALAVPALGLYEISIYSVRMIEKGQAKPPGQGP"
     gene            1480..2910
                     /gene="serS"
                     /locus_tag="VNY06_03710"
     CDS             1480..2910
                     /gene="serS"
                     /locus_tag="VNY06_03710"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011441629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HWX13963.1"
                     /translation="MYDIKWIRDNAESFDRGRKRRGLEVLAAQLLALDDKRRAAIAKS
                     QAAQERRNAASKEIGAAMKAGDADLAKALKIEVGDLKTALAEFEAEEREAILALDKAL
                     AEIPNTPLDDVPDGRDENDNVELRRVGEPRAVDFAPKEHFDIGEALGLMDFETATKIS
                     GARFVVNKGPLARLERALSQFMLDLHTGEHGYTEVNPPILVRYEAMFGTAQLPKFEED
                     QFNASKGTLRDQAFLKTVLERIENVCEVAAYKEQGSVPIDVLKQNIELFKWDVFKEHF
                     WLIPTAEVPLTNLVRDSILDEKELPIRVTACTPCFRAEAGAAGKDTRGMIRQHQFTKV
                     ELVSITTPEASLAEHERMTLCAEEVLKRLKLPYRVVTLCTGDLGFASQKTFDIEVWLP
                     GQTRYREISSCSVCGDFQARRMNARYRPEGAKNTRFVHTLNGSGVAVGRALVAVLENY
                     QNSDGSVTVPEALRVYMGGIERIAKA"
     gene            2921..3682
                     /gene="surE"
                     /locus_tag="VNY06_03715"
     CDS             2921..3682
                     /gene="surE"
                     /locus_tag="VNY06_03715"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012456446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HWX13964.1"
                     /translation="MRILITNDDGIHAPGLALMERIAKSFSDDVFVVAPENDQSGVAH
                     SLTINDPLRLRKISERHFAVKGTPTDCVIMGVRKLLEGATFDLVLCGVNNGQNVADDV
                     TYSGTVAGAMEAASLGLPAIALSQAYGPIGREESFWECAETHAPSLIGQILAVGIPQY
                     VVINVNFPACPPDSVQGIAVSAQGRRDAQAAVIDERADGRGIPYYWISFTRGSSAPGH
                     GTDLEALAQKKISVTPLKLDLTDEPALTRLALSLR"
     gene            3700..4389
                     /locus_tag="VNY06_03720"
     CDS             3700..4389
                     /locus_tag="VNY06_03720"
                     /inference="COORDINATES: protein motif:HMM:NF013315.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA adenine N-6-methyltransferase family
                     protein"
                     /protein_id="HWX13965.1"
                     /translation="MSFEMPPPPLMEQASETQDKAEFLMMLRARGIQDLNLLRAVETV
                     PREIFVPHRFADLARRPIALPLRCGQTLPEPWVTARMIEALAPSPRHRVLEIGTGSGY
                     ATALLARLSREVLSIERFQSLAIEAEERLAQLAIANAAIAFGDGLAISPQIGPFDRII
                     VQGLLAEVPEKLFAALGEMGVLVAARPGPNTATRQHIARITRNDAGGIDESPVCPCRM
                     QALLPGEAAAL"
     gene            4519..>4902
                     /locus_tag="VNY06_03725"
     CDS             4519..>4902
                     /locus_tag="VNY06_03725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HWX13966.1"
                     /translation="MNEIVGVFRARFALRLALVGAAAGLLAGCSSSERLSDPLSNPFQ
                     SSARVDGQASAGGALGAPVASANSSQQNLRAASVVPPPDLGRAAGWSAQGGSPIVVAQ
                     GENAQMLSHRYGVPVDALIRTNGLSV"
BASE COUNT         1015 a         1391 c         1420 g         1076 t
ORIGIN      
        1 gccggcgtat ttgagtgacc tttcctcatg ttcgaatttg acgccggcaa gctaattatc
       61 atcgcaatcg tcgcgctcat cgtcattggg cctaaggaac tcccccgcgt catgcggcac
      121 gtgggtgcag cattagctaa aatgcagcgc atggcggccg aatttcgcgg ccagttcctg
      181 gacgccatgc gagaatcaga aatcgatgac atcaagtccg aagtgaaaac gcttacagat
      241 agttcgaaga cggattttgg tattgatccg cttgcgcaaa tcaaagcaga actgacgcaa
      301 gcacttgaag cggccgaaaa gtccacgtca cctcctgaaa ctggtgcggt ggccttagtt
      361 tctgcgcaaa acggggaaat ggccgccact cattctgtat cgccatgcac gcctgatgga
      421 tcagaaatgg cgggcttatc tgtcgccaag cctggcgatg ctccagcggc ccctgaggat
      481 aagaccgccg ctgccggggc aatcgatgcg gagatgcggg cgcttgccga tgcgatcgcc
      541 gccaagattg gggaggccgc gcccggaggg gccgcagatg aacacgtcaa ggcgcaggac
      601 aatcggtgac cgttcgagac accttaactc ggccagatcc agacgctcca cgctcgggaa
      661 aaaaagtctt tcatgacaga agccgatata gaggcgacaa aagcgcccct gatggatcat
      721 ttgattgagc tgcgcgcgcg gctgatcaag gcactcgttg cctttttggt tatgttcatc
      781 gtctcgttct attttgccaa ggatatttac aatattcttg tggtgccatt cgaacatgcg
      841 gccggtccag acgcgcgtct gatctatacc gcgccgcagg aatatttttt tacccaaatc
      901 cgagtcgcac ttttttccgc tgcgtttctc tcttgtccgg tggttttcgc gcagctttat
      961 gcctttgtcg cgccaggcct ctacaagcat gaacgcaagg ccttcgcacc ttatcttatg
     1021 gccacaccac ttttcttcgc cgcgggcgcg ctgctcgttt acttcgtggt catgccgaat
     1081 ttgctgctgt ttttcattgg catgcagcag gccaaggagc cggggcgggc gcagatcgaa
     1141 cttctgccgc gcgtcagcga atatttatcg ctgatcatga ctttgatttt cgctttcggc
     1201 ataaccttcc aactgcccgt ggtcttgacg cttcttgggc gcgtcgggat cgtgacgtcg
     1261 gattttttgc ggcggcagcg gcgttatgcg gttgtgctcg tttttgttct agccgcaatc
     1321 ctgacgccgc ctgacatatt ttcgatgctg gcgcttgctg tgccagcgct cggcctttac
     1381 gagatttcga tctactccgt ccgcatgatc gagaaaggtc aagcgaaacc gcccggacag
     1441 gggccataat ccttggcgga gttttagaga atcgctctaa tgtatgacat caaatggatc
     1501 cgcgacaacg cggaaagctt tgaccgcggg cggaaacggc gcggtcttga agtgctcgcg
     1561 gcgcaactac tggcactcga cgacaagcgc cgggcggcga ttgccaaatc gcaagccgcg
     1621 caagagcgcc ggaacgccgc gtcgaaggag atcggcgccg cgatgaaggc aggcgacgcg
     1681 gacctcgcca aggccttaaa aatagaggtc ggtgatttaa agacagcgct tgcggagttt
     1741 gaggccgagg aacgcgaagc gatcttggcg cttgacaagg cgcttgccga aattcccaac
     1801 acgccgctcg acgatgtgcc ggatggccgc gacgagaatg acaatgtcga gctgcgccgc
     1861 gtcggcgagc cgcgcgcggt ggattttgcg ccaaaggagc atttcgacat cggcgaggcg
     1921 ctcggcctga tggatttcga gacggccaca aaaatttccg gcgcgcgatt tgtcgtcaat
     1981 aaagggccgc ttgcccgttt agaacgcgcg ctttcgcagt tcatgctcga tctgcacacg
     2041 ggcgagcatg gctacacgga agtcaacccg ccgatcctcg tgcgctacga ggccatgttc
     2101 ggcacggcgc aattacccaa attcgaggaa gatcagttca acgctagcaa aggaacgcta
     2161 cgcgatcagg ccttcttgaa gactgttctg gagaggatcg aaaatgtttg cgaagtcgct
     2221 gcatacaaag aacaaggtag tgttcccatt gacgtactga agcagaatat tgagcttttt
     2281 aagtgggatg tctttaaaga gcacttctgg ctcatcccca ccgccgaagt gcccctcacc
     2341 aatcttgtgc gtgattcaat tctcgacgaa aaggagctgc ccatcagggt caccgcctgt
     2401 acgccctgct tccgggccga ggcgggcgcg gctgggaaag acacgcgcgg catgatccgc
     2461 cagcatcaat tcaccaaagt cgaactcgtt tcgatcacca cgcccgaagc ttcgctcgcc
     2521 gagcacgagc gcatgacttt atgcgccgag gaagtcttga agcgcctaaa acttccctat
     2581 cgagtcgtca cactgtgcac cggcgacttg gggttcgcct cgcaaaaaac ctttgacatc
     2641 gaggtctggc tgccggggca aacccgctat cgcgaaatct catcctgctc ggtttgcggc
     2701 gatttccagg cgcggcgaat gaatgcgcgt tatcggccgg agggcgcaaa aaacacgcgg
     2761 ttcgtgcaca cgctgaacgg ttcgggcgtt gccgtcgggc gtgcgctggt cgcagtgctc
     2821 gaaaattacc aaaacagcga tggttctgtc accgtgccgg aggcattgcg cgtctacatg
     2881 gggggcatcg aaaggatcgc aaaagcttga ggccgcgccg atgcgcattt tgatcaccaa
     2941 cgacgacggc attcatgcgc cggggctcgc gctgatggag cgtatagcta aatccttttc
     3001 agacgatgtt tttgtcgttg ctcccgaaaa tgatcagtcg ggtgttgccc attcgctgac
     3061 catcaacgat cccttgcggc tacgcaaaat ctcggaacgg catttcgcgg ttaagggaac
     3121 gcctaccgac tgcgttatta tgggcgtgcg aaagctcctt gaaggcgcca ctttcgatct
     3181 cgtcctatgc ggcgtaaaca acggccagaa cgtcgccgat gatgtgacct attccgggac
     3241 ggtcgcaggc gcgatggaag cggcaagcct tggcctgcct gcaattgctc tttcccaggc
     3301 ctatggccca attgggcgcg aggaaagctt ttgggaatgc gccgaaaccc atgcgcccag
     3361 cctcatcggc cagatccttg cggtgggcat tccgcaatat gtggtaatca atgtgaattt
     3421 tccggcctgt ccgccagatt ctgttcaagg gatagcggtc tcggcccagg gtcggcgtga
     3481 cgcgcaagcg gccgttatcg acgagcgggc cgatgggcgc ggcattcctt attattggat
     3541 ttcgttcacg cggggcagtt cggcaccggg acacggcacc gaccttgaag ccttggcgca
     3601 aaaaaaaatt tcggtgacac cactcaaact cgatttgaca gatgagcccg cgctcacgcg
     3661 ccttgcgctt tccttgcgct aattgttgca ggcctgccta tgtccttcga aatgccgcca
     3721 cccccgctga tggaacaagc gagcgagaca caagacaagg cggagttttt gatgatgctg
     3781 cgtgcccgcg gcattcaaga tcttaatctt ttgcgtgcgg ttgaaaccgt cccgcgcgaa
     3841 atcttcgtgc cgcatcgctt tgccgatctc gcgcgccggc cgatcgcgct gccactgcgc
     3901 tgtggccaga cccttccgga accatgggtt acagcgcgga tgattgaggc actggcgccg
     3961 tcgccgcgcc accgcgtact ggaaatcgga accggatctg gctacgcgac ggccctgctt
     4021 gcgcggcttt cgcgcgaagt gctttctatc gagcggttcc agagcctcgc aatcgaggca
     4081 gaagagcgcc tggcacagct cgcgattgcc aatgcggcga tcgcctttgg cgacggcctt
     4141 gcgatttcac cgcaaatagg tccgttcgac cggatcattg tacaggggct gcttgccgaa
     4201 gtgccagaga aacttttcgc ggcgctcggg gaaatgggcg tgcttgttgc cgcgcggcca
     4261 ggcccgaata ccgcaacgcg gcagcatatc gcgcggatca cgcggaacga tgctggcggg
     4321 atcgacgaga gcccggtttg cccatgcagg atgcaagcat tactaccggg cgaagcggcc
     4381 gcgctctgaa cggtcacgaa gcacttcaaa taaacataat tttgtctctt ctttcgtgaa
     4441 tatttgttca tctttaaacc gtcctttaac ttactccgca gttaatggac caaggctact
     4501 atgtcggtgg gttgcgtcat gaatgaaatc gttggtgtat tccgcgcacg tttcgcgctt
     4561 cgtttggcct tggtcggcgc agccgccggt ttgcttgccg gatgctcaag ctccgaaagg
     4621 ctgagtgacc ccttatctaa tccttttcaa agttcagcga gagttgacgg tcaggcgtcg
     4681 gcgggcggcg cgctcggtgc acccgtcgca agcgccaact catctcaaca aaacctaaga
     4741 gctgcgtccg ttgtgccacc gcctgatctt ggacgcgccg ctggctggtc ggcgcaaggc
     4801 ggatcgccga ttgtcgtcgc gcaaggcgaa aatgcgcaaa tgctttccca tcggtatggc
     4861 gtgccagtcg atgcgctcat taggacaaat ggactctccg tg
//
LOCUS       DATMPU010000162         5562 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_3851791,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000162 DATMPU010000000
VERSION     DATMPU010000162.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 5562)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5562)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5562
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3851791"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            543..1592
                     /locus_tag="VNY06_03730"
     CDS             543..1592
                     /locus_tag="VNY06_03730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020185641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter substrate-binding
                     protein"
                     /protein_id="HWX13967.1"
                     /translation="MLAGAPLPLKSEEVNILNVSYDPTRELYSDINGLFAERYKAWTG
                     VSVTFEQSHGGSSKQARSVIDGLKADVVTLALAWDIIAIERAGLIKPGWQQKFPYNSS
                     PFTSTVAFLVRKGNPKHIKEWADLTRPGVQVIVANPKTSGAGRWSFLALWGATTKAKT
                     HDFSIPEELKGTQAAARTAKDFPVYESAETRASIEKFYNNNVPVLDTGARGSTVTFAQ
                     KQLGDVLLNWENELWLAREEFGEDKFDIVYPSRSILGEPPVAVVDEVVDKKGTRDVAE
                     AYLKFLYTSEAQSLAAEHHYRPRDVNVLQKYSVELPKIPLFTVDETFGGWERAQAEFF
                     AEGALFDQIYRPSKQ"
     gene            1589..2557
                     /locus_tag="VNY06_03735"
     CDS             1589..2557
                     /locus_tag="VNY06_03735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019404336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HWX13968.1"
                     /translation="MTENTKARPLPPSPRNLFSASPRPSRLIPGLPLTLGYTLFYLSA
                     LVLIPLGGMVFKTAQLSASEFWQTITDPIVVASFKLTFGASALAAIINGVFGLIVAWV
                     LVREPFPGRRIFDALIDFPFALPTAVAGLTFSQLYLPDGWIGGLGRHITGLLNSIAAF
                     AERGPLLAENALSWLNFPYSNTNAGIVIVLIFVGLPFIVRTVQPVLRDWDTEHEQAAM
                     SLGANRWTIFWRVVFPEIFPAWLSGLALAFARAIGEYGSVIFIAGNIPGKSQIAPLQI
                     VTKLDDFRYAQATAIGVVLLALSLLILLAINGLEWWARHHELRPAR"
     gene            2664..3647
                     /locus_tag="VNY06_03740"
     CDS             2664..3647
                     /locus_tag="VNY06_03740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013419017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HWX13969.1"
                     /translation="MSHPLLHKILVAVTIGFLTLFIVIPVANIFAQALSKGLDSYVAV
                     FHVANPPKGKELTPMERRKVAFARNQAEKTWGSIRLSVGIAAIVVPLNLIFGLAAAWS
                     VTKFRFKGRSLLIALIDLPFSVSPVIAGLIFVLFLGRNGIFGPLVANWTWPDPLSLYW
                     RGFAQDGWPLGFARSYSGVIFTPLAIALASIFVTFPFVARSLIPLMDTQGSDEEVAAL
                     SLGASGWRTFFKVTLPNIKWALLYGVILCTARAFGEFGAVSVVSGHIDSNDTMPLRVE
                     KLWNEYNNQAAFTVASLLTLLAVVTLLLQLIVERKGGKPAIAAVEGEGAIR"
     gene            3644..4747
                     /locus_tag="VNY06_03745"
     CDS             3644..4747
                     /locus_tag="VNY06_03745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015599086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TOBE-like domain-containing protein"
                     /protein_id="HWX13970.1"
                     /translation="MSIEVCRVNKTFNQFKALDNVSLKVETGELVSLLGPSGCGKTTL
                     LRMIAGMDTADVGSGAILFDGRDVSGDEVGKRRIGFVFQHYALFKHMSVFENVAFGLR
                     AKPRRLRLREAEIRVKVGDLLQLVQLQNFASRFPSQLSGGQRQRVALARALAVEPSVL
                     LLDEPFGAHDATVRRELRRWLRKLHQRIRVTTILVTHDQEEALEVSDRVAVMNKGRIE
                     QYASPEEIYDNPANPFVFKFLGSYNLFRARKAFVGDTAAAIPASADGVTLVRPHDVEI
                     SRENNNDGSGIAARIKHIAFAGSLVNVELTRLDNEESVDAALPAHDYKELGLKPEDRV
                     FIRLRNATSFDEDNVLAEEVAPTLLPPEVLMRG"
     gene            complement(5069..5200)
                     /locus_tag="VNY06_03750"
     CDS             complement(5069..5200)
                     /locus_tag="VNY06_03750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX13971.1"
                     /translation="MTFLLVAAAIAGFVAWYKVNQDYLGEYFSSFRDFYRYVSFHLK"
BASE COUNT         1147 a         1558 c         1536 g         1321 t
ORIGIN      
        1 ccgcaacctt gccttgaaag gcgcgctgga cgcggaaacc tccggggcgt cccgtaattg
       61 cgcgatctca ggcgctctca cggcgccatt gctcgcaggg cgcaagtccc tcgcgcatca
      121 aataagatta gatatgtttg cacggcgcgt ttgattgatc ccaatcacac cgatagcaaa
      181 tcggcttcat tgagcggcgg aagcgtggtt cgcggcgatc ctgcgatctt tatggaaact
      241 gcaccgattt tgcgccgata aggcagcgaa aacatgcttg accgcttccc cgcccttgac
      301 aatctaattg tctatagact tactcgttta cgaggctgcc ataccatcaa atacttagat
      361 aggcattgga aagggccggg gagatttcgc ttatggccag tcgcgttaat ctacttttag
      421 ctggcgccgc cccggccgga ccagacacca aggatcaatt tgctcggcga caccccgaaa
      481 tatcgttcgg ccagcccggg cggtcatggc gggccgccct gcgaagcttt tccctaggac
      541 ttatgctcgc aggcgcgccg ctccccttga agagcgaaga ggtgaatatc ctcaacgttt
      601 cctacgatcc cacgcgggag ctttattcgg acatcaatgg tttattcgcg gagcgctaca
      661 aagcctggac tggcgtcagc gtcaccttcg agcagtcgca tggaggatcg agcaagcaag
      721 ctcgttctgt catcgatggt ttgaaggccg atgtagttac tttggcattg gcgtgggaca
      781 ttatcgcaat cgaacgcgca ggcctcatca aacccggctg gcagcagaaa tttccctaca
      841 attcctctcc cttcacatcg acggtcgcct ttctcgtgcg caagggaaat ccaaaacaca
      901 tcaaggagtg ggctgatttg actcgccctg gtgtgcaggt gattgtggct aatcccaaga
      961 cgagcggggc cggacgttgg tcgtttcttg cgctttgggg tgcaacgacc aaggcgaaga
     1021 cgcatgattt ttctatccct gaggaactca aagggaccca ggcggcagca cgaacggcga
     1081 aggattttcc tgtttacgaa agcgccgaaa cccgcgcgtc gattgagaaa ttctacaaca
     1141 acaacgtgcc cgtgctcgac actggcgcgc gcggctcgac cgtcactttc gcccaaaaac
     1201 agcttggcga tgtcctgtta aactgggaaa atgagctttg gctcgcaagg gaagaatttg
     1261 gcgaggataa attcgacatc gtctatccgt cacgcagcat tttgggcgag ccgcccgtgg
     1321 ctgtcgtcga tgaggtcgtt gacaagaagg gcacgcggga tgtcgctgaa gcctatttga
     1381 aatttctcta cacgtccgag gcgcagtctc tggcagccga gcatcattat cggccgcgcg
     1441 acgttaacgt gcttcagaaa tatagcgttg aactgcccaa gatcccgctc ttcactgtgg
     1501 acgaaacgtt tgggggctgg gaaagggcgc aagcagaatt ttttgccgaa ggggcgctgt
     1561 tcgatcagat ttataggcct tccaaacaat gaccgaaaac acgaaggcgc ggcctcttcc
     1621 tccatctccg agaaatctct ttagcgcgtc gccccgcccg tcaaggctta tcccagggct
     1681 gccgctcact ctcggctaca cgctgtttta tttgtccgcg cttgttctaa tcccccttgg
     1741 cggcatggtc ttcaagacgg ctcaattgag cgcgagcgaa ttctggcaaa ccattaccga
     1801 tccaatcgtt gttgcgtcct ttaaactgac ctttggcgca tcggcgctgg ccgcgatcat
     1861 aaacggcgtc ttcggattaa tagttgcctg ggtgcttgtg cgcgagcctt ttcctgggcg
     1921 gcgcatattc gatgcgctta tcgattttcc atttgccctt ccaacagccg tcgccggtct
     1981 taccttcagc cagctgtatc tacccgatgg ctggatcggc ggtttggggc gtcacatcac
     2041 gggcctccta aattctatcg cagctttcgc cgaacgcggc cccctgttgg cggagaacgc
     2101 tctctcctgg ctgaattttc cttattccaa caccaatgcg ggaattgtga ttgtcttaat
     2161 ctttgttggc cttcccttca tcgtgcggac cgtgcagccg gtactgcgcg attgggacac
     2221 ggaacacgaa caagcggcta tgagccttgg ggccaatcga tggacgattt tctggcgcgt
     2281 cgttttcccc gaaatttttc cggcgtggct cagcggcctg gcgttggcct ttgcccgcgc
     2341 catcggcgaa tatggctcgg ttattttcat tgccggaaac attcccggca agtcgcagat
     2401 tgcgccgctt cagatcgtta ccaagctcga cgattttcgg tatgcgcaag caaccgcgat
     2461 cggtgtggtc ttgctcgctt tgtcgctgct tatcctgctt gcgatcaatg gtctcgagtg
     2521 gtgggcgagg catcatgagc ttcgacccgc aaggtaaaat cggagatccc cagagtcagc
     2581 gcacttttgg attgagggtt ggtttgatgg cccgaacgct caagcaaact ccccgcgccg
     2641 cgatgcccgt ccccgcggct ccaatgtcac atccgctgct ccacaaaatc ttggttgcgg
     2701 tgacaattgg ctttttgacc ctcttcatcg tgattccagt ggcaaatata ttcgcgcagg
     2761 cgctttcaaa ggggctcgac tcttatgttg ctgtgtttca tgtggcaaat ccgccaaagg
     2821 gcaaagagct aaccccaatg gagcggcgca aggttgcttt cgcgcgcaat caggccgaga
     2881 aaacctgggg ctcgattagg ctttccgttg ggatcgcggc catcgttgtt cccctcaatc
     2941 tcatctttgg gcttgccgcc gcttggtcgg taacaaaatt ccgcttcaag ggccgttccc
     3001 ttttgattgc gctgatcgat ctgccatttt ccgtatcgcc ggtcattgcg gggttgattt
     3061 tcgtgctgtt tcttggccgc aatggcattt ttggccccct tgtcgccaat tggacgtggc
     3121 ctgacccact ctcgctctat tggcgcggct ttgcgcaaga cgggtggccg ctaggcttcg
     3181 cccgaagcta tagcggagtt atttttacgc cattggcaat cgctttggct tcgatctttg
     3241 tcacatttcc tttcgttgcc cgctctctaa ttccactcat ggacactcag ggatctgatg
     3301 aggaagtggc ggcgctgtcg cttggcgcct ccggctggcg caccttcttt aaggtgacct
     3361 tgcccaatat aaaatgggcg ctactttatg gcgtaattct ttgcaccgcg cgggcctttg
     3421 gcgagtttgg cgcggtctcg gtggtctcgg ggcacattga ctcaaatgac acaatgcctt
     3481 tgcgtgtcga gaaactttgg aatgagtata acaaccaggc agcctttacc gtcgcatcct
     3541 tgctgacctt gctcgcggtg gtcactcttc ttcttcagtt gatcgtcgag cgcaaagggg
     3601 gcaagccagc gattgccgcg gtcgaggggg aaggggccat acgatgagca ttgaagtttg
     3661 cagggtcaac aagaccttca atcagtttaa ggcgctcgat aatgtcagcc tcaaggtcga
     3721 gaccggagag cttgtctcgc tgctcgggcc aagcggctgc ggcaagacaa cgctgttgcg
     3781 catgatcgcg ggcatggaca cagctgatgt tggcagcgga gcaattcttt tcgacggccg
     3841 cgatgtttcc ggcgatgagg tcggcaaaag gcgcatcggt tttgtgttcc agcactatgc
     3901 gctcttcaaa catatgagcg tcttcgaaaa tgtcgctttt gggctgcgtg cgaaacctcg
     3961 ccgcctgagg ctcagagaag cagaaattcg cgtcaaagtc ggcgatttgc tgcagcttgt
     4021 tcaattacag aattttgcca gccgctttcc ctcccaactc tccggcggtc agaggcagcg
     4081 tgtggcgctc gcccgggcgc tagcagtcga gccttccgtc ttgttgctcg acgagccttt
     4141 tggcgcgcat gacgcgacgg tccgccggga actcaggcgc tggttgcgta aattgcacca
     4201 gaggatccgt gtcacgacca ttttggtcac ccatgaccag gaagaggcgc tggaagtgtc
     4261 ggatcgggtc gcggtcatga acaagggacg aatcgagcaa tacgcatcgc ccgaggaaat
     4321 ttatgacaat ccggccaatc cgttcgtttt caagtttttg ggaagttaca atctcttccg
     4381 tgcccgcaaa gcttttgtcg gtgacaccgc cgcggcgatt ccggcaagcg ccgatggcgt
     4441 gactttagtg cggccgcacg acgtcgaaat cagccgcgag aacaataatg atggctccgg
     4501 cattgcagcc agaatcaaac atattgcctt tgccggctcc ttggtgaatg tcgaactcac
     4561 gcgtcttgac aatgaagagt ccgtggacgc cgcgctcccg gcgcatgact acaaggagct
     4621 tggcctaaaa ccagaggaca gagtgtttat caggctacgc aacgcaacct cctttgacga
     4681 ggacaatgtt ttggcggaag aggttgcgcc cacgcttctg ccgccggagg tcctcatgcg
     4741 cggctaaaat tggcgccccg gcagcgaatt tgtttgatgc cggagcgcaa tcccacgggg
     4801 ttacgcgttt tgctggcccc gaaaccttac gccttgccga ggttcggcgg cggcgggggg
     4861 ccccagccat caaggaaagc tcgcgctctc tatcgcgtgc ggcgcgaaca accggagcca
     4921 tcatacccgg gaagaccgcc ggaagaccag gaaggttgcc cgtcaggtct cgcgacatcc
     4981 gaaagaggcg ccgccagata ttgatgcgta agagctcgtc cggcggcgtg atgttcaagt
     5041 gttggccgca atattgcgtt tggccttgtt atttcaggtg aaaactcacg tagcggtaaa
     5101 aatcccgaaa ggagctgaag tactcgccaa gataatcctg gttgaccttg taccacgcga
     5161 cgaagccagc gatcgccgcg gccacgagca agaacgtcac aaattgtctt gttctgtttt
     5221 tcaaaggctt ctcgcagtcc tctttgcctc tcgcctagac cgcccagact gaacccaaca
     5281 aaccggcaga gcaagcgcgc aagccaattc taaattatct tgcccaacgc gtccaggagc
     5341 acaagcgcca tcttgctgga tgggactaat accaaatctc ggaaaggctg actcctgcga
     5401 tcggattccc gaatcagcgt cgatgtgatt caccgtggca aacagctttt gaaaggtttg
     5461 ccatgtctac tccaattcgt tcaaagagaa gctctgaata tttacggact tcccatcgaa
     5521 agagataccg ccctgcttta cgaagccaac ggatggttgc cg
//
LOCUS       DATMPU010000163         4869 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_1076045,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000163 DATMPU010000000
VERSION     DATMPU010000163.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 4869)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4869)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4869
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1076045"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(207..1163)
                     /gene="tal"
                     /locus_tag="VNY06_03755"
     CDS             complement(207..1163)
                     /gene="tal"
                     /locus_tag="VNY06_03755"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020174294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HWX13972.1"
                     /translation="MPSKLDQLKTMTTVVADTGDLDAIRRFGAVDATTNPSLILKAAR
                     MPHYGPLLDKAVLWGHDHKQMTSEVTDRLTLNFGLELSKIVPGRVSTEVDADLSFDLE
                     GTIAKARAIIEAYERLGLSRAHVLIKIAATWEGIRAAEILQSEGIDCNMTLVFSLTQA
                     AACADAGVFLISPFVGRILDWHVKNEGKTYAADTDPGVLSVRQIYYYYKAHGFKTDVM
                     GASFRNKGEIEALAGCDRLTISPQLLDELSQGMSELPRRLDPKQAAVHAPAKLSLDEK
                     TFRFMLNEDAMASEKLGEGIRTFARDLRTLRELVSKRLQEAA"
     gene            complement(1343..2107)
                     /locus_tag="VNY06_03760"
     CDS             complement(1343..2107)
                     /locus_tag="VNY06_03760"
                     /inference="COORDINATES: protein motif:HMM:NF024503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HWX13973.1"
                     /translation="MTAAAKSLLPPEESPELRRWLISAAIVIALHGSLLLWLMHKRDI
                     SAAGAPPAVVMIDLPAMDVAPASEVLPDAVEGPKMTKAQPQEEVETPEAMAVPELPPA
                     HKPEAVLMPPPKPKLKPKKNEIPKSTVKQTHEPPVPRTSAPKHSEAARGQMSAALSRG
                     TAGSAASIASWRTQINAHLLRHKPTGGEGAGVVTISFSLARSGALIGAHLTGSSGSSA
                     LDQRALEMVRRANPFPAAPPEVTGGAFPFTVPVRFR"
     gene            complement(2104..2601)
                     /gene="exbD"
                     /locus_tag="VNY06_03765"
     CDS             complement(2104..2601)
                     /gene="exbD"
                     /locus_tag="VNY06_03765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006201178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein ExbD"
                     /protein_id="HWX13974.1"
                     /translation="MAIRLTPQEDALEATHEINVTPFIDVMLVLLIVFMVAAPLSTVD
                     VNVDLPASTAEPAPRPDKPLFLTVKPDLTLALGETPIDRSTLQSALDAQTQGDKEERV
                     FVRADRLVPYGEVMEVMNLLRAAGYLKIGLVGLEQAPASRSDASGSAAGEAQSPGALP
                     AGTKP"
     gene            complement(2607..3638)
                     /gene="exbB"
                     /locus_tag="VNY06_03770"
     CDS             complement(2607..3638)
                     /gene="exbB"
                     /locus_tag="VNY06_03770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006201177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tonB-system energizer ExbB"
                     /protein_id="HWX13975.1"
                     /translation="MIGRRTGLTAQRGGRSGFILAILAPLFGAILCVGPVIAESAPIT
                     TEAERSAPAAEPSANLAKTPRLAASPEDIPAAGGVPPTPVEPAKKQLYTPLRSIDALL
                     PSALPHDLSPWGMFLSAVPIVQAVMMALAFASLVTWTVWLAKTIELWNAKRLARRGFE
                     VLASASSLHAAQNILAGAPSPVARFATAAAEEGNRSNGLAAEGIKDRAAILLSRIEAQ
                     AGRKITRGTGILATIGATAVFVGLFGTVWGIMDSFIGISRTNTTNLVVVAPGIAEALL
                     ATAMGLVAAIPAVVIYNSFARSITGYRAQLADAAAEVLRHLSRDLDRDALARREGDSP
                     IVALRQLAE"
     gene            complement(3886..4725)
                     /locus_tag="VNY06_03775"
     CDS             complement(3886..4725)
                     /locus_tag="VNY06_03775"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012589487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HWX13976.1"
                     /translation="MDESLAAVLRWYVDMGVDIAVDDTPHDRFAEATSTARANAPISA
                     AVPPPQAAGQRVSAGPAAAMPESAIEASAWDFAAAAQGLDDLRDRLARFEGCGLKATA
                     TQLVFGDGNPAAKIMVVGEAPGADEDRQGVPFVGRAGRLLDKMLSSIGLDRSKVYIAN
                     IVPWRPPGNRTPTPVETAACLPFTRRQIELVDPQILVCLGAASAQTLLGGKEGIMRMR
                     GRWHAYPTAKREIRALAMLHPAYLLRQPAQKRLAWQDLRVLAKEIGSLGLFSEVNTER
                     DWS"
BASE COUNT         1026 a         1417 c         1465 g          961 t
ORIGIN      
        1 cgacgaatgg attgagcatc gaaaccaaga tccgaaacca ttcacatggg ccgccgccgc
       61 caaaaccatt ctcgtcaagc accgccgagc caaaaaaatt ctcgccaaag caggacgcaa
      121 atgaatgagt cagaacacta gagaagccag ctgaaaatca ccaagaaacc aactgccgaa
      181 acccccgcgc ctatttgagg ccctgcctag gctgcttctt gcagccgctt tgacaccaat
      241 tcgcgcagcg tgcggaggtc gcgggcaaag gtccttatgc cttccccaag cttctcgctt
      301 gccatagcat cctcgttcag cataaagcgg aaagtctttt cgtctaaaga gagttttgcc
      361 ggggcgtgaa cggcagcctg ctttggatcg agccggcggg gcagttcgct catgccttgc
      421 gagagctcat caagcaattg cggtgagatg gtcagccggt cgcaacccgc cagagcctcg
      481 atctcccctt tgttgcggaa tgacgcgccc atgacatcgg tcttgaaacc atgggccttg
      541 taataataat agatttgacg gaccgaaagg actccgggat cggtgtccgc cgcataggtc
      601 ttgccctcgt ttttcacgtg ccaatcgaga atgcggccga cgaaaggcga gatgagaaag
      661 acgccggcgt cggcgcaagc cgcggcctgg gtcagcgaga agaccaaggt catgttgcag
      721 tcgatgccct cgctttgaag aatttccgcg gcgcggattc cttcccaagt ggcggcgatc
      781 ttgatcagga catgcgcgcg gctcaacccc agtctttcat atgcctcgat gatcgcgcga
      841 gctttcgcga tcgttccttc caggtcgaaa gatagatctg catcgacttc tgtggagacg
      901 cggcccggca cgatcttgga taactccaaa ccaaagttca gcgtgaggcg gtcggtgacc
      961 tcgctcgtca tttgtttgtg gtcatgaccc caaaggacgg ccttgtccaa caatggacca
     1021 tagtgcggca tgcgcgccgc cttgaggatg agggagggat tggtggtcgc atcgacggcc
     1081 ccgaagcggc gaatcgcgtc cagatccccg gtatcggcga cgacggtggt catagttttt
     1141 aattgatcga gctttgaagg cattggcgtt gcggtctggg taaaacgaag gaattctcta
     1201 tataagaagg aattctctat ataaaacgcg tgggcgcaga gattgttgcg gaggacatac
     1261 gcccacgagc acccattgcg caattattta tgcaacagag gactgtagga ctgagaatac
     1321 cgctagcgat tgctgctgct gctcaacgga agcggaccgg gaccgtgaat ggaaacgcgc
     1381 ctccagtgac ctcagggggt gcggccggaa aagggttcgc gcgcctgacc atttcgagcg
     1441 cccgctgatc aagggctgag gagccagaac tcccggtgag atgcgctcct atgagcgcgc
     1501 cacttcttgc caagctgaag gagatcgtaa cgactcccgc tccctcgccg ccggtcggtt
     1561 tatggcgaag cagatgggcg ttgatttgag tgcgccacga tgcaatggat gcggcggagc
     1621 cagccgtgcc tcggctcagt gccgcggaca tctgcccgcg cgcggcttct gaatgtttgg
     1681 gtgcgcttgt ccgcgggacg ggaggctcgt gcgtttgctt cactgtggac ttgggaattt
     1741 cgtttttctt cggcttcagt ttgggctttg gcgggggcat taagaccgcc tcaggtttat
     1801 gtgcaggggg aagctcgggg accgccatcg cttcgggggt ctcaacttcc tcctggggtt
     1861 gggccttggt catcttcggc ccttcgacgg cgtcgggcag aacttccgac gctggcgcga
     1921 catccattgc cggaagatct atcatcacca ctgccggagg ggctccggcg gcgctgatgt
     1981 cgcgtttgtg catgagccac aaaagcagtg agccatgaag ggcgatgacg atcgctgcgg
     2041 aaataagcca ccgccgcagc tcagggcttt cctcgggcgg cagcaaggat ttcgcggccg
     2101 cggtcaaggt tttgtcccgg ccggaagggc ccctggggat tgtgcctccc cagctgctga
     2161 tccacttgcg tcagagcggc tagcaggcgc ctgctccaaa ccgacaaggc cgatcttcaa
     2221 atagcccgcc gcgcgtaaaa ggttcatcac ctccatgacc tcgccataag ggacaagccg
     2281 atccgctcga acgaagacgc gttcttcctt gtcgccttgg gtttgcgcat cgagggcgct
     2341 ctggagagtg ctcctgtcga tgggcgtttc gccgagtgcc aaagtaaggt ccggctttac
     2401 agtcagaaac agcggcttgt cgggacgggg cgcgggctct gcagtagacg cgggaagatc
     2461 gacattgaca tcgacggtcg agagcggcgc agcgaccata aagacgatca acagaaccaa
     2521 catgacgtcg atgaatggcg tcacattgat ttcgtgggtt gcctccaggg cgtcttcttg
     2581 cggcgtgagc cggatagcca tggaatttac tcagccaact ggcgcagggc aacgatgggc
     2641 gaatctccct cgcgtcgcgc gagggcgtcg cggtcgagat cccgggacaa atggcgcaga
     2701 acctcggccg cggcatcggc gagctgggca cggtaaccgg tgatcgagcg ggcgaaggaa
     2761 ttatatatca cgacggcggg aatcgccgcg acaagtccca tggcggtggc gagcagggct
     2821 tcggcaatgc ccggcgcgac aaccacgaga ttggtggtgt tggttcgcga aatgccgata
     2881 aaactgtcca tgattcccca gacggtccca aacaagccaa caaacacggc ggttgcgccg
     2941 atcgtcgcca aaatccccgt tccccgggtg attttccggc ccgcttgggc ttcgatccgg
     3001 gaaagcaaaa tggccgcgcg gtcctttatc ccttcggcgg caaggccgtt ggagcggttg
     3061 ccttcttcgg ccgcagcagt ggcgaagcgc gcgacgggtg agggcgctcc tgcaagtata
     3121 ttttgggctg cgtgcaggct cgacgcggaa gccaggactt caaacccgcg ccgcgccagc
     3181 cgctttgcat tccaaagctc gatcgttttc gcgagccaga cggtccaggt cacaagggac
     3241 gcgaaagcaa gcgccatcat gacggcctgc acgatgggca cggcactcaa aaacattccc
     3301 caaggggata ggtcgtgcgg caatgccgaa ggcaggagtg catctattga acgcaaaggc
     3361 gtgtagagtt gcttcttcgc aggttcaact ggagtgggtg gtacaccgcc cgctgcgggg
     3421 atatcctctg gcgaagccgc gagccggggc gtcttagcta gatttgccga cggttccgct
     3481 gccggcgccg accgctcggc ttccgtcgta ataggagcgc tctcggcgat caccgggccg
     3541 acacaaagga tcgcgccaaa caatggcgcg agaatcgcca aaataaatcc actgcgcccg
     3601 ccgcgttgag ccgtcaggcc ggttcttctg ccaatcattt agttccactc ctaaagcgtc
     3661 cggctagccc aaatctgttg agccggtaag caccagctct gataacccga attaaggcgg
     3721 cagcaaagat catggcccct ctctacggac tgcttgaggg tctcgacaca ttaaagcgca
     3781 aattcatcag tttatcggaa aaacaataac ttatactttc gcgtaaatgt gcgcgaacaa
     3841 gcccacaagc cgtaaaatag gcctgtcacg tcggaggtcg aagattcaag accagtctct
     3901 ctcagtatta acttcagaaa acagccctaa gctaccgatt tcctttgcga gaacccgcag
     3961 gtcctgccat gcgagccgct tttgcgccgg ctggcgcaga agataggctg ggtgcagcat
     4021 ggcgagcgcc cggatttccc ttttagccgt tggataggca tgccaacgcc cccgcatacg
     4081 catgatgccc tccttgccgc cgagcaaggt ttgcgccgac gcggccccga ggcagacaag
     4141 aatttgcgga tcgacaagct cgatttggcg gcgcgtgaag ggcaggcacg ctgctgtttc
     4201 gacaggggtc ggcgtgcggt ttccaggcgg ccgccaaggc acgatattgg cgatatagac
     4261 cttgctccgg tcaagcccga tggacgagag catcttatcg agcaatcttc ctgcccggcc
     4321 gacaaagggc acgccttggc ggtcctcatc cgcgccgggg gcttcaccga ccaccatgat
     4381 cttcgccgcg gggttgccat cgccaaaaac gagctgcgtc gccgtcgcct tcaggccgca
     4441 gccttcgaag cgcgcaaggc gatcgcgcaa atcatccaga ccttgcgcgg ctgcggcgaa
     4501 atcccaggca ctcgcctcaa tcgcgctttc gggcatggcc gcagccggtc ctgccgaaac
     4561 acgctggccc gccgcctgag gtggaggcac agcggcggat attggcgcgt ttgctcgcgc
     4621 cgtgcttgta gcttccgcaa atcggtcatg gggcgtatcg tcaaccgcga tatcgacccc
     4681 catgtccacg taccagcgca agactgcggc caaactttca tccataatag aaccgtctta
     4741 tatattcacg ggaataagga tcaaaaccca ttgtgagtga cgccgaagtc aaattgccgc
     4801 cacgcgaaag catggatttc gatgtcgtga tcgtcggcgc tggccctgcg ggtcttgctg
     4861 ccgcgattc
//
LOCUS       DATMPU010000164         2671 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_1999865,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000164 DATMPU010000000
VERSION     DATMPU010000164.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 2671)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2671)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2671
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1999865"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..595
                     /locus_tag="VNY06_03780"
     CDS             <1..595
                     /locus_tag="VNY06_03780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010682258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HWX13977.1"
                     /translation="RDFLTVVLIGRSQRQWPDNWIYIHDPRVKVGRVQNFRSWSPEMV
                     PDNESTCLGLEYFCSEGDALWSMTDADVIAVAKKEIEEIHLMCGDEINDACVVRQKKA
                     YPVYDETYLDNLRMIRLEIQTRFPSLHLVGRNGMHKYNNQDHAMMTGMLTAENILAGR
                     TLFDVWQVNEDAEYGEAGLSGAREALQSTRIVPKKAA"
     gene            600..2582
                     /locus_tag="VNY06_03785"
     CDS             600..2582
                     /locus_tag="VNY06_03785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX13978.1"
                     /translation="MSPTSHATTPLLREMPIQGGAPDPAGTPWFQQKNGPSFLQGVRR
                     LLAWPLLAAAKNSCPIPLIVLSVFLLSRPYGGIVHDAHIYIGRALADLDPNGVGRDIM
                     FVYDGQFGFSLFRQVADGMVTCFEPGTAAKTLAVLAAFAWLFGIRAFARQFASGATVW
                     VAVIFSVLLPNNYGAPYPLGFAELIAVPRPFTEALVFASLAALATKRDGVSLLFIVAA
                     ALLHPIMAMAGMAVWASVRCMEDKRWIWCCAIGGALLVGAGSVGLPLVDRLFTAIDPS
                     LRGLHETRSPFLFPSHWPIESFPPLLVQATTLAIAASFEKGRHRTILAAILLIGLSGI
                     AVTAIFGDWLSSLLIVQAQPWRMAWLMAAAAAMALGVVSVELWRRGQSGRLVLAFVVL
                     CWSLNTQFAVAGPAAILALCFHFGASRFAPGLTPKLVPCTWIFTVIMAAIWKVRLLAY
                     PWHFSMEAPAGHGNFELLIVRGYLVLPLCALAVYFAVAKPRISQLAQAGTAALLLMAV
                     LALWDHRAPAQRTVEANQRPPELMRLVNQCQGDVYWIDGHVEAWVVLGRPQWATPLQG
                     VPIIFSHALAAEWQRRMRILMDLRLADQKSFSPWSDPASADRPQLSRQGVMQLCARDD
                     APAWIIAPIDHGAEFPAGLQMTLWRLPEPQIDQSRW"
BASE COUNT          478 a          762 c          796 g          635 t
ORIGIN      
        1 ccgcgatttt ctcaccgtcg tcctgatcgg ccggtcgcag cgtcaatggc ccgacaactg
       61 gatttatatc catgacccga gagtcaaagt cggcagggta caaaacttcc gctcatggtc
      121 gccggagatg gtccctgaca atgaatcgac ttgcctcggt ttggaatatt tctgcagcga
      181 gggcgacgcg ctctggagca tgacagacgc ggatgtcatt gccgttgcca agaaagaaat
      241 cgaagaaatc cacttgatgt gcggcgacga gatcaacgat gcctgcgtcg tgcggcagaa
      301 gaaagcctat cccgtctacg acgaaaccta tctcgacaat ttgcgcatga tccggctcga
      361 gattcaaaca aggtttccct ctcttcatct cgtcggccgc aatggcatgc acaaatacaa
      421 caaccaggac catgccatga tgacgggcat gctgaccgcc gaaaatattc tggccgggcg
      481 aacgctcttt gatgtctggc aggtcaatga agacgccgaa tatggcgagg caggtttgtc
      541 aggcgcgcgc gaggctcttc aaagcacgcg tattgtgccg aaaaaagcgg cctgaggacg
      601 tgagcccgac ctcgcacgcg acaacccctt tgcttcgcga aatgccaatc caaggcggcg
      661 ctccggatcc cgccggaacg ccctggttcc agcagaaaaa tggcccaagt tttttgcaag
      721 gcgtgaggcg gttgcttgcc tggcctcttc tggccgcggc aaaaaactcc tgcccgattc
      781 cgctcatcgt tctgagtgtt tttctgctga gccgccccta tggggggatt gtccatgatg
      841 ctcatattta catcggccgc gcgcttgccg atctcgatcc aaatggggtc ggccgcgata
      901 tcatgttcgt ttatgatgga caatttggtt ttagtctctt ccgccaggtg gcggacggga
      961 tggtcacgtg tttcgagccg ggaacggccg cgaaaaccct ggcggtcctg gcggcgttcg
     1021 catggttatt tgggatccgc gccttcgccc ggcaattcgc cagcggcgcc acggtctggg
     1081 tggccgtgat tttttcggtc cttttaccaa ataattatgg cgcgccctat ccactgggtt
     1141 ttgccgagct gatcgctgtt cctcgcccgt tcaccgaagc cttggtgttt gcctcgctcg
     1201 ccgcgcttgc cacaaagcgc gacggtgttt cgctcttgtt cattgtggcg gcagcgctgc
     1261 ttcatccgat catggcgatg gcggggatgg ccgtctgggc gagcgtccgc tgcatggagg
     1321 acaagcgctg gatttggtgc tgcgctatcg gtggcgctct attggtaggg gcgggcagcg
     1381 tcggcctgcc cttggttgac cggcttttca cggcaatcga tccgtccttg cgaggcttgc
     1441 acgagacgcg aagcccgttt ctgtttccga gccattggcc gattgagagt tttccgccgc
     1501 ttctggttca ggccaccacg ctggcgatcg ccgccagttt tgagaaaggg cggcaccgga
     1561 cgatccttgc cgcaatcctc ttgattgggt taagcggcat cgccgttacg gcgatttttg
     1621 gtgactggct ttcatcgctt ctcattgttc aagcgcaacc ttggcgcatg gcatggttga
     1681 tggccgcggc ggcggcgatg gccttgggtg ttgtttctgt cgaattgtgg cggcggggcc
     1741 aaagcggacg cctggttttg gctttcgtgg tgctttgctg gtcgttaaac acccaatttg
     1801 cggtcgctgg ccccgccgct atcctcgcgc tttgctttca tttcggcgcg tcacggttcg
     1861 cgccggggct tactccgaag ctcgttcctt gtacatggat ttttaccgtt atcatggcgg
     1921 cgatttggaa ggtccggctg ctcgcctatc cttggcactt ttcaatggaa gcgccagcgg
     1981 ggcacggcaa tttcgaactt ctcatagtca gaggctacct tgttcttccg ctttgcgcgc
     2041 ttgctgttta ttttgccgtc gccaagccgc gcataagcca gcttgcgcaa gccggtacag
     2101 cggcgctctt gctcatggcg gtgcttgccc tttgggatca tcgggcccct gcccaacgca
     2161 cggtggaagc gaatcagcgg ccgccggagc tcatgcggct cgtcaatcaa tgccaaggcg
     2221 atgtgtattg gatcgatggg catgtcgaag cttgggttgt ccttggccgg ccgcaatggg
     2281 ccacgccgct tcaaggcgtc ccgattattt tttcgcacgc gctcgccgcc gaatggcagc
     2341 gccggatgcg aattctcatg gatttgaggc tagcggatca aaaaagcttc tcgccatgga
     2401 gcgatcccgc gagtgcggac cggccgcaat tgtcgcggca aggcgtgatg cagctttgcg
     2461 cgcgagacga tgcccccgca tggatcatcg cgccgatcga tcacggcgct gagttccctg
     2521 cggggcttca aatgacactt tggcgattac ccgagccaca aattgaccaa agccgatggt
     2581 gattatgtct ggcaagaaat tgatgcctat gggttgatcc catgcgccgg gcaggcgcag
     2641 tctcagcggt ctgaacagcg cgaataaacc a
//
LOCUS       DATMPU010000165         8050 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_1682958,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000165 DATMPU010000000
VERSION     DATMPU010000165.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 8050)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8050)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8050
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1682958"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(342..893)
                     /locus_tag="VNY06_03790"
     CDS             complement(342..893)
                     /locus_tag="VNY06_03790"
                     /inference="COORDINATES: protein motif:HMM:NF019886.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HWX13979.1"
                     /translation="MALRDDLELCSAQLYRFACALVCGHPGPCAQAADLVRGTLSSAL
                     KGSPASRLTGHNLRIRLYTVLIKCHRDGRTVGSIGAKARVEIDNFHIKHSIGGNTSVI
                     ASSQDNFSAALLDLGLDEREALLLVALEGFDYGQAARILKISRSVLVGRLARARAALS
                     ETVPVESSGRPVKARPTYLRLVK"
     gene            1124..1972
                     /gene="fabI"
                     /locus_tag="VNY06_03795"
     CDS             1124..1972
                     /gene="fabI"
                     /locus_tag="VNY06_03795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012384102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004318 - enoyl-[acyl-carrier-protein]
                     reductase (NADH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HWX13980.1"
                     /translation="MTLPGISTPGLQTSGILAGKRGLVMGVANNRSIAWGIAKAAREA
                     GAELAFTYQGESLEKRVRPLAKELDAKVVGDCDVTDIRALNAAFQEIAGLWRTLDFVV
                     HCLAFSDKDQLTGRYIDTTTENFVNSLLVSCYSFTAIAQRAEKLMPNGGAILTLTYYG
                     AEKWMPHYNVMGVAKAALESSVRYLAADLGERNIRVNAISAGPIKTLAASGIGDFRYI
                     LKWNEYNTPLRRTVTIEEVGESAAFLLSPMARGITGEILHVDAGYHIVGMKNPAAPDI
                     AVLAKD"
     gene            2228..6409
                     /locus_tag="VNY06_03800"
     CDS             2228..6409
                     /locus_tag="VNY06_03800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012590041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904091 - non-ribosomal peptide synthetase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Pls/PosA family non-ribosomal peptide
                     synthetase"
                     /protein_id="HWX13981.1"
                     /translation="MATGRTAPSRGAGGEAEGQGLADLKMAQKLESGFSRCVMLQRPM
                     NAQKLLEKGAPPAATSGMVLKGPVRPDLIRDELLCEIFLATVSKSPSAIAMVTEEGKL
                     NYAEVDARAEDIARGLVRKGVRPGRVVGLWMPRGPELLIAQIAISKTGAAWLPFDADA
                     PAERIAVCLSDAEAFGILTAPAFATRLATPCPTWTTPILAADTAEVSDLERINARALG
                     ARPEDPAYIIYTSGSTGTPKGIVISGRNICHYLRAANESYGVHAEDIVFQGASVAFDL
                     SMEEIWLSYLVGASLFVATAELLSETDKLPDVMEANRITVLDAVPTLLALLPRDVATL
                     RLIILGGEACPAGLGARWSRPGRKIFNSYGPTETTVVATVVELCPELPVTIGQPIANY
                     TCYVVSEGLELLPSGTEGELLIGGPGVAKGYLRRERLTREKFISNPFFTDASDPVLYR
                     SGDAVEIDPNGHILFRGRIDDQVKVRGFRVELGEIEAKLAELSGVTRAAVVLRDDNGN
                     EQLVAFVVPSFESEIDAKFLRSQLRAILPPYMVPARFEVLLTLPKLASGKIDRKALKQ
                     IELAHCDSIESQEKPRTETEAKLLEAAKRVFPPQAIPFDADFFTDLGGHSLLAARFIS
                     AVRETPILARITLQDVYAARSLRAMAELLDRKWANSAGAEDLSFAPPPRLRRFLCGCA
                     QALALPVILSLVTAQWLGVFVSYMLLTGPAASLVEEILSLIGVYMCINIATVAIVIAA
                     KWLVIGRTKPGRYPLWGIYYFRWWLVKRFLGLVHIKWFQSSPLIRMYLSALGAKIGKD
                     AIIGEIEPGAVDLISIGAGASIGSIANLSNARVEGNELIIGTIDIGDKVHIGSSCVIE
                     ENTIIEEGAELGDLTAIRAGSRIGAWEVWDGSPGREVGQVDRAALDEPPAASALRRSA
                     MMVLYLISLLAIPPLGLMPIIPAFWVFDRIDDVIGTADIDRTLYMLSIPIMAWPTSFV
                     MILVTVAFIAVCRWVILPRVREGVYSVHSWFYLRKWVVTLATEVTLETLSSLYATVYM
                     RGWYRLMGAKIGKDAEISTNLSGRYDLVEIGEKCFIADEVVFGDEEIRNGWMYLKGVK
                     TGPRVFVGNSAVIPTGSEIPANALIGIKSKPPANSMMQKGDTWFGSPPIKLPVRQAFD
                     AGGETWTYEAPRWKKIARACFEAVTISLPTMLFITFGTWAVEWFSASLLEGKYFTLVS
                     EFTVASVAICIALTLAVIALKWVTMGRYEPMMRPLWSWWAMRTEAVAVVYWGMAGKVL
                     LDHLRGTPFLPWIMRLFGMKFGRGVYMDTTDFTEFDCVSVGNHTSLNALCALQTHLYE
                     DRVMKVGRVFVGSGVTVGAGSTVLYDTQVSDYAQLGPLTVVMKGEHIPASSAWAGAPA
                     EPRAAPGQIIAKSAA"
     gene            complement(6416..7189)
                     /gene="pgeF"
                     /locus_tag="VNY06_03805"
     CDS             complement(6416..7189)
                     /gene="pgeF"
                     /locus_tag="VNY06_03805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011504260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HWX13982.1"
                     /translation="MLVAPIVLKASLLNRPGLTHGFFTRRGGVSTGVYASLNGGVGSK
                     DDPAAVAENRRRMAEHLQIDPLRFLVPYQTHSPDALIVTKAFAERPRCDALVTAKPGL
                     GLGVTGADCGMVLFADVKARVIAAAHAGWKGALAGVLEATLDAMETLGAQRTSTAAVL
                     GPTIAQKSYEVGADFATKFIAESIDHGRFFAASARNGHFMFDLTGFIAMRLRSAGIGA
                     FEDLARDTYTEPECFYSYRRSVHKNEPDYGRQIAAIALI"
     gene            complement(7183..>8050)
                     /locus_tag="VNY06_03810"
     CDS             complement(7183..>8050)
                     /locus_tag="VNY06_03810"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HWX13983.1"
                     /translation="LFVELGPGRGTLMADALRAARVAPDFLSAIDIHLVEMSEILQQC
                     QRMALKDLGESVSWHASTEEMPDGPAIIVANEFFDCLPIRQFARSTTGWHERLIGLNG
                     EGRLCFGLAPGPEPRLTAPGEPGQVFEAGLVGARLMSRLAERICLQGGALLLVDYGYD
                     VPQRGATLQAVRSHRFADPLGDPGEADLTAHVDFCALARAARAAGAIVHGPVPQGEWL
                     TRLGLYERAAKLREKASGSQNAAIDSALQRLAGEWQGSPTEDMGRLFKVVAVTEPRLS
                     ALSGFETAAVPC"
BASE COUNT         1691 a         2364 c         2266 g         1729 t
ORIGIN      
        1 ccaggactat gccccgagag gagtaccccg ctcacgaaac caaccaccgt caaatgggca
       61 agcccgcatt cgcgccacgg acgggaggtc tgccccccct tttcccaggt gacagcagag
      121 atgtccgcag cagctgcgcg gcttcagtcg gcgggaaggc agcacggatt gcttcagttt
      181 gccgctgcca cgtttcgaca cgggccgcat ccacaggaca agcagcaagg tgagcccgga
      241 cagcttcttg ccgttcgcga tctaactcgc catcaacata tccatgcagt tccgcttcac
      301 tcaccggggg cttgatcccg ctgctacgca taactgtccc gctatttgac gagccgtaaa
      361 taagtggggc gcgctttgac tggccgcccg gaactttcta caggtaccgt ctcactcaaa
      421 gcggcacgtg cacgcgccag tctcccgaca aggacgctgc gcgaaatttt caggatccgc
      481 gccgcctgcc catagtcgaa gccttctagc gcaaccagca acaaagcttc ccgctcgtca
      541 agcccgaggt ccaatagcgc agccgaaaaa ttgtcttgag atgaagcaat taccgaggtg
      601 tttccgccaa tactatgttt gatatggaaa ttgtcgattt ccacgcgagc tttagcgcct
      661 atgctaccca ccgtccgccc gtcgcgatgg catttgatga gcaccgtata aagacgaata
      721 cggagattgt ggccggtcag ccgcgaggcc ggcgatcctt tgagcgccga cgataaggtg
      781 ccacgcacaa ggtcggcagc ttgcgcacag ggccctggat ggccacagac aagcgcgcaa
      841 gcaaaccggt aaagctgcgc cgaacacaac tctaaatcgt cgcgcaatgc cacttttgca
      901 ttcccttgcc gccgcgtttt ccatcttaac tgaggaccac ccgaatctgg gacgtcatac
      961 acctctagac tagatgcgcc ataaccatcg gcagaggtgg cttctcggcc aagtctttac
     1021 taactcatgc ggtcttgcgc taaacgcgcg cagcccttaa acgcagcaca cgccaccgcc
     1081 gttccaattg acggcttctt gacctcaatg atccggatcg agcatgacgt taccgggaat
     1141 atcaacgcct ggactccaaa cctcgggaat cctcgcggga aaacgcggct tagtcatggg
     1201 agttgccaac aaccggtcaa tcgcctgggg tatcgccaag gctgcccgcg aggctggcgc
     1261 cgaacttgcc ttcacctatc aaggtgaatc gctagaaaaa agggtcaggc cgcttgcaaa
     1321 agagcttgac gctaaagtcg tgggcgattg tgatgtcacc gatatacgtg ctttaaatgc
     1381 tgcatttcaa gaaatcgccg gcctctggcg aactctcgac tttgtcgtcc attgccttgc
     1441 tttttccgac aaggatcagc tcacaggccg atatatcgac acaacgaccg aaaactttgt
     1501 aaattcgctc ctagtctctt gctattcctt tacggcgatc gcccagcgag ccgagaagct
     1561 tatgcctaac ggcggcgcca tcttgactct gacctattat ggggccgaga aatggatgcc
     1621 gcactacaat gtcatggggg ttgccaaggc agcgctcgag tccagcgtgc gctatcttgc
     1681 cgccgatctc ggcgaacgca atattcgcgt taatgcgatt tcagccggtc cgatcaagac
     1741 attggccgcc tcagggatcg gcgatttccg ctatatccta aagtggaatg aatacaatac
     1801 tccattgcgg cgcaccgtaa cgatcgaaga agtcggtgaa agcgctgcct ttctgctttc
     1861 acctatggcg cgcggcatta ctggcgagat cctccatgtg gatgccggtt accacattgt
     1921 tggcatgaaa aatcccgccg cgccggatat agctgtacta gctaaagact gagacattca
     1981 cggtcggttc acccagatag ttgtaaagac ccatggcgtc acggactcac gcactcatct
     2041 ccagttcgat tgtgggttgg gcagctgtta tagatacgcc aatgctccgg atgcgcaaaa
     2101 acgtgcatcc tgtcttgcgt agcgacgaca attgcagcga cagggaacaa gctgacgtca
     2161 ggcactcaga aagctctcgt ggaatttatt ctgccgcaag cgacttcttg acaaaggctg
     2221 gcgccgattg gcgaccggta gaaccgcgcc atcaaggggg gctggcggcg aagctgaggg
     2281 tcaaggcctt gccgatctta agatggccca gaagcttgaa tctggcttta gcaggtgcgt
     2341 tatgctgcaa aggcctatga acgcccaaaa actcctggaa aaaggtgcac caccagcggc
     2401 tacgtccggg atggttttga aaggcccagt tcggccggat ttgatccgcg acgagttgct
     2461 ctgcgaaatc tttttggcaa cggtttcgaa aagcccctct gcgattgcca tggtaacaga
     2521 ggaaggcaaa cttaactacg ccgaggtcga cgcaagagcc gaggacatcg cccgcggtct
     2581 cgttcgaaaa ggagtacgtc cgggccgggt cgttggactt tggatgccgc gcggacccga
     2641 acttcttatc gcccaaattg cgatctctaa gaccggcgcg gcttggctgc cgtttgacgc
     2701 ggatgctcct gcggagcgaa tcgctgtttg cctttctgac gccgaagcct tcggcatctt
     2761 gactgcgcct gcctttgcta cgcggctcgc gactccttgc cccacttgga cgacccctat
     2821 cctcgcggcc gataccgcgg aggtctccga cctagagaga atcaacgcgc gcgctcttgg
     2881 ggctcgcccg gaggatccag cttatatcat ctacacctct ggttcgaccg gaaccccaaa
     2941 gggaatcgta atttcaggac gcaatatctg ccactatttg cgcgctgcca acgagtccta
     3001 tggcgttcac gcagaagata ttgtcttcca gggcgcctcg gttgcctttg atctttccat
     3061 ggaggaaatt tggctttcct acctcgtagg cgccagcctg ttcgtggcca ctgcggaact
     3121 cctcagcgag accgacaagc tgcccgacgt catggaagcc aatcgtataa cggtgctgga
     3181 cgccgttccg accctgcttg cgctcttacc gcgtgacgtc gccactttga ggctgatcat
     3241 tctgggcggc gaagcctgcc cggctgggct cggcgcaaga tggtcgaggc ccgggcgcaa
     3301 aattttcaat tcctatggac cgacggaaac gacagttgtc gcaacggttg tagagctctg
     3361 ccctgaactc ccggtcacaa tcggccagcc aattgcgaat tacacttgct acgtcgtgag
     3421 tgagggtctc gaacttcttc cctctggaac cgaaggcgag cttctcatcg gcggtccggg
     3481 ggttgcaaaa ggctatttgc gccgcgagcg gctgacccgc gaaaagttta tcagcaatcc
     3541 atttttcact gatgcttccg atcctgtctt gtaccggtcg ggcgatgccg tggagattga
     3601 tccgaacggc catattttgt ttcgcggccg aatagacgac caggttaaag tacgcgggtt
     3661 tcgcgttgaa ttgggcgaga tcgaagcaaa actcgccgaa ctctcaggtg ttacgcgagc
     3721 cgcggttgtc ctgcgtgacg ataacggcaa cgaacagctt gtggcctttg tggttccctc
     3781 cttcgagagt gaaatcgacg cgaaattctt gcgtagccaa ctccgggcga ttttaccccc
     3841 ctatatggtc ccggccaggt tcgaagtcct cctgaccctg ccgaagctcg cgtccggcaa
     3901 gatcgaccgc aaagcgctaa agcagatcga acttgcccat tgcgacagca ttgaatccca
     3961 ggagaagccg cgcaccgaga cagaagcaaa acttctcgaa gcggccaagc gcgtttttcc
     4021 accccaggcc attcctttcg atgcggactt cttcaccgac cttggcgggc attccctgct
     4081 cgcagccaga ttcatctctg cggtgcgcga aacacccatt ctggcacgga tcaccttaca
     4141 ggacgtctac gcggcccgtt cgctgcgggc catggccgaa cttctcgacc gcaaatgggc
     4201 taattccgct ggggctgagg atctgtcttt cgcgccgcca cctcggttgc gccgtttcct
     4261 atgcggatgc gcgcaggctc ttgcgcttcc cgtcattctt tccctcgtca ccgcgcaatg
     4321 gctgggcgtg ttcgttagct acatgttgct cacgggcccc gcggcgagtc tcgttgagga
     4381 aatcctctcg ctcattggcg tctatatgtg cattaatatc gccacggttg cgattgtcat
     4441 cgcagcaaag tggctggtga tcggaagaac caaacctgga cgctaccctt tgtggggcat
     4501 ttattatttc cggtggtggc tcgtgaaacg cttccttggg ctcgtgcata ttaaatggtt
     4561 ccagagttct ccgctgatac gaatgtatct ttctgcgctc ggagccaaaa tcggcaagga
     4621 cgcgatcatc ggcgagatcg agcccggcgc agtcgacctc atctcaatcg gcgcgggcgc
     4681 aagcattgga tcgatcgcaa atctttccaa tgctcgagtg gagggcaatg agcttattat
     4741 tggaacaatc gacattggcg acaaggtgca cataggctcc tcctgcgtga tcgaagaaaa
     4801 tacgatcata gaagagggag ccgaacttgg cgacctaacg gccatccgcg ctgggagccg
     4861 catcggcgca tgggaagttt gggacggatc gccgggccgc gaggtaggcc aagtggatcg
     4921 cgccgcgctg gacgaaccgc cggccgcgtc tgcgttgcgc cgaagcgcta tgatggtgct
     4981 ttatctcatc tcacttctgg ctataccccc gctcggctta atgccgatca ttccggcttt
     5041 ttgggtattc gaccggatag acgatgtaat cggcaccgcc gacatcgacc gcacgctcta
     5101 tatgctctcg atcccgatca tggcgtggcc gacgtccttc gtcatgattc tcgtcactgt
     5161 cgccttcatc gcggtttgcc gctgggttat tttgccgcgc gtgcgggaag gggtttattc
     5221 ggttcactcc tggttttatt tgcgcaaatg ggtggtgacg cttgcaaccg aggtgacgct
     5281 cgaaacactg tcgtccttat acgcaacagt ctatatgcgc ggttggtacc ggctgatggg
     5341 cgccaaaatt ggcaaggacg cggagatctc gacaaatctc tctggccgct acgacctcgt
     5401 cgagattggg gaaaaatgtt tcattgctga cgaagtcgtc ttcggtgacg aagaaatccg
     5461 caacggctgg atgtatctga aaggggtgaa gaccggcccg cgcgtgttcg ttggcaacag
     5521 tgcggtgatt cccacaggat ccgaaattcc cgccaatgcg ctgatcggaa ttaagtccaa
     5581 gccgcccgcg aattcgatga tgcaaaaggg cgatacctgg ttcgggtcgc cgcccatcaa
     5641 actgccggtc cggcaagcat tcgatgccgg aggcgagacc tggacctacg aggcgccgcg
     5701 ttggaagaag atcgcgcgcg cttgcttcga ggctgtcaca atttcgcttc cgacgatgct
     5761 gttcatcact tttggaacct gggcggtgga gtggttcagc gcgagcctgc tcgaaggtaa
     5821 gtatttcaca ttggtttcgg aatttactgt tgcctcggtc gccatatgca tcgccctaac
     5881 tctggcagtt atcgcattaa aatgggttac gatggggcgg tatgagccga tgatgagacc
     5941 cctatggtct tggtgggcca tgcgaaccga agcggtcgcg gtcgtctact ggggaatggc
     6001 gggaaaggta ttgctcgatc atttgcgtgg gactcccttc ttgccctgga tcatgcgcct
     6061 cttcgggatg aaattcggcc gcggagtcta tatggacaca actgacttca cggaattcga
     6121 ctgtgtgagc gttggcaacc atacttccct gaacgcgctt tgcgcgttgc aaacgcacct
     6181 ttatgaggac cgcgttatga aagtcggcag ggtcttcgtt ggcagtggcg tgaccgtcgg
     6241 cgcgggctca actgtccttt acgacacgca agtctccgac tatgcgcagc ttggaccttt
     6301 gaccgtcgtg atgaagggcg agcacatccc cgccagttcg gcatgggctg gcgcaccggc
     6361 tgagccccgc gcggcgccgg ggcaaatcat cgcgaaatcg gccgcataaa gtatttcaaa
     6421 tcaacgcaat tgcagcgatt tgccggccgt agtccggctc attcttgtgg acagaacggc
     6481 ggtatgaata aaagcactca ggttcggtat aagtgtcgcg cgcaagatcc tcgaaggcgc
     6541 cgatgcccgc tgaccgtagc cgcattgcaa taaagcctgt cagatcgaac atgaaatggc
     6601 cgttgcgcgc cgatgccgcg aagaatcggc cgtggtcaat tgattccgcg atgaacttag
     6661 tggcgaaatc tgcgccgact tcataactct tttgcgcgat ggtaggcccg agcaccgctg
     6721 ctgtcgaagt tcgctgcgcc ccaagcgtct ccatcgcgtc aagcgttgct tcgagcacgc
     6781 ccgcgagtgc ccccttccac ccggcgtggg ctgcggcgat tacgcgcgcc ttcacatcgg
     6841 caaaaagaac catgccacag tcggcacctg tgacaccaag acccagaccc ggctttgctg
     6901 tcaccagagc atcgcagcgc gggcgttcgg caaacgcttt tgtcacgatc agcgcatccg
     6961 gcgaatgggt ttggtatggg accaggaaac gaaggggatc aatttgaaga tgctccgcca
     7021 tccggcggcg gttttccgcg acggctgccg gatcatcctt tgaaccaacg ccaccattaa
     7081 gcgaggcata aacgccggtc gaaacgccgc cccgcctcgt aaagaaaccg tgggtgaggc
     7141 ccggcctgtt gagaaggctt gccttgagaa cgatcggtgc tactagcatg gtacagctgc
     7201 cgtctcgaag cccgacagcg cggaaagacg tggctccgtc acagcgacca ccttaaaaag
     7261 tctccccata tcctccgtcg gcgagccctg ccactctcct gcgaggcgct ggagcgccga
     7321 atcgatggct gcgttctggc tgccgctggc cttttcgcgc aacttcgccg cgcgttcgta
     7381 aagtccgagg cgcgttagcc actcgccttg tgggaccgga ccgtggacta tggcgccggc
     7441 cgcacgcgcc gcccgagcaa gagcgcagaa atccacatgt gccgtgaggt cagcttcgcc
     7501 tggatcacct aggggatcgg caaagcgatg gcttctcacg gcctgcaaag ttgcgccccg
     7561 ctggggaaca tcgtagccat agtctacaag aaggagcgcc ccgccttgga ggcaaatgcg
     7621 ctcagcaagt cgcgacatca gccgcgcccc gacaaggcca gcctcgaaga cttggcctgg
     7681 ctcgccgggc gcggtgaggc ggggctcggg gcctggtgca aggccaaagc acaaacgtcc
     7741 ttcgccattc agtccaatga gcctttcatg ccagccggtg gtgcttcggg cgaattggcg
     7801 tataggcaag caatcaaaaa attcattggc cacaatgatc gctgggccgt caggcatttc
     7861 ctctgtgctc gcatgccaac tgacggattc gcccaaatcc ttcaacgcca tacgctggca
     7921 ttgctgtaat atctcgctca tctcgacgag gtggatgtcg attgcagaaa gaaagtccgg
     7981 cgcaacgcgc gcggcgcgta gggcgtccgc catcagcgtg ccccgccctg gccccaattc
     8041 gacaaataga
//
LOCUS       DATMPU010000166         4604 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_3547741,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000166 DATMPU010000000
VERSION     DATMPU010000166.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 4604)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4604)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4604
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3547741"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(888..1790)
                     /locus_tag="VNY06_03815"
     CDS             complement(888..1790)
                     /locus_tag="VNY06_03815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016919304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EcsC family protein"
                     /protein_id="HWX13984.1"
                     /translation="MNFPTSEVQPDRASVDVLPDGASRLSPEDRAALQRAISELERSS
                     LAIRLSAILGRQASALASFVPAPLAETANRAAKAAIRSSLNVAMKSLAGKPLRDRRRM
                     HKSLAVLAGAAGGAFGVSGLPLELPFSTTIILRSIADIARAEGHDLEDPRAVLACLEV
                     FALGGQAASDGAFKFRNIHAQVRLKEGAILETGYFALRAILAKSVSEAASHLAGGTTS
                     MAAPALVRFVGQIGAHFGVAVSQKFVAQTVPLIGAAGGAAINYAFADHFQSIARGHFT
                     VMRLERRYGERIIRTEYDQMRRAA"
     gene            2207..2854
                     /locus_tag="VNY06_03820"
     CDS             2207..2854
                     /locus_tag="VNY06_03820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003609489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like cysteine peptidase"
                     /protein_id="HWX13985.1"
                     /translation="MKVRTATWALAPPLLWAFLAGLVAMTPSCAASAGAPRPAERAFM
                     PIGGPTSVPYGWVDFCRRYHGECDGGLIVPRDINLTPQMMQVIDRVNKWVNAYVKPVS
                     DPEQWGVIDQWDYPYDRRGDCEDYALFKRKILIEEGLPRQGLLMTVVKDEQDVGHAIL
                     TVKTNSGEFVLDNLNDEVKPWNRTGYRFVKRQSQADQNLWVQIGDPTTTPDYVSH"
     gene            complement(3116..3718)
                     /locus_tag="VNY06_03825"
     CDS             complement(3116..3718)
                     /locus_tag="VNY06_03825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008131770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HWX13986.1"
                     /translation="MMQLYWAPRTRSLRALWALEEAGVPYERIQLDLSSGDQKRVEFC
                     AINPMAKVPALTDGPIAVAESGAICAYVAEACPEARLAPPPGDSARGRYLQWLFFSPG
                     CIEQAFMAKAYKISPRPESAGFGDYDRVFDVLEEAVAAGPFLLGERFSAADVMIGTDL
                     YFGIDVFKLVYARPALRAYVDRCLARPALQRAKVIDAGGS"
     gene            complement(3744..>4604)
                     /locus_tag="VNY06_03830"
     CDS             complement(3744..>4604)
                     /locus_tag="VNY06_03830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020175859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HWX13987.1"
                     /translation="RGLTDFAARLQSAEVNKRTIESLAAAGAFDELESNRARVYAGAE
                     TILAFANRQEEEKLAGQSQLFGGENLDAIILPTTEPWPLAERLRREFESVGFFLSGHP
                     LDAYATVLARLRVQRWAEFEAAVKEGATTGRLAATVLDRHERRTKSGTKMGILQLSDQ
                     SGHYEAILFQERLNQYREVLEKGISVLLGLQASLEGEEVRARIVSAEPLDRAASRCGK
                     GLRIVLHDKNPLGEIVGQLRQRGEGEVLLILLTSSGEVEVKLPGKYYVSPQAVGALKA
                     IPGIVAVEHI"
BASE COUNT         1035 a         1372 c         1246 g          951 t
ORIGIN      
        1 ccagcgtcat ctcttacatt gcggtacgtc ttgagcgatc acttgacgcg gctcgtttgt
       61 ttgtcgatgc cctcgacgaa gagtcgttgg cgcgacaaag ccggatcacc aaaacgattg
      121 cgcgggacat tctgtgctca atggggggcg gggagaacac cagcggctag ggcgaagccg
      181 cgcgaggccc gcccgcgggg acgatgttac ggcccgacaa atcggatcgc tcggcctttg
      241 gcatcggtga tcatttcccc agccgcgatc ccgcgttctg ttgctgagcg tcccgtaacg
      301 ataccgctcg tcacgccacg cggacgtttg agtgtccaag gctgccagcg ctttccgttc
      361 gttcttggaa agccccttta ctcctgatgg ctttctgaaa tcttccgggt cgtggcgatc
      421 gcccgaatag ccaatgaaaa cgcgaattcc ggccttcgtc atcaccacat cgcccttgcg
      481 caggctatcg tcctccatga tatcgactgg ctcctgcgcc tggacggctt ggttcgttct
      541 cgcggcgacg attattttct tatgcgcgct gtgtgaggcg ctcttgtgtg accgcctatg
      601 gctccagtag tttccgcgcc ggcgaaatcc gggctcgggc cgccacgggc catcgtccct
      661 ataggagggc ggccggaata gctgtccgaa aatgtcgtca aaaaatcccg cttgcgcgct
      721 tccagggcac gccaggatca agcctaagaa aattgtgacc actgccacga cgaaacggca
      781 gcgccaaaaa ctagacctca taataacatc cgtaagctac aaatgccggc gcggacgcca
      841 cctttgacta aagcagaacg cgcgaaacgt cgaggagttg cccgatatca agcggctcgc
      901 ctcatctgat catattccgt gcggattatt cgctctccgt agcgccgttc gagccgcatc
      961 accgtaaaat ggccgcgtgc gattgattga aaatgatccg caaatgcata attgatcgcc
     1021 gcgccgcccg cagcaccaat cagtggcaca gtttgggcca caaatttttg cgagacggct
     1081 acgccaaaat gcgctccgat ctgcccgaca aagcggacga gcgccggcgc agccatgctt
     1141 gtcgtgccgc cagccagatg gcttgccgct tcggacaccg atttcgccaa aatggcccga
     1201 agcgcgaaat agcccgtttc aagtatggca ccttctttca ggcgaacctg agcatggata
     1261 ttccgaaatt tgaaggcccc atccgatgcg gcctgtcccc caagcgcgaa cacctcaagg
     1321 caagcgagca cggctctcgg atcctcgagg tcgtgtccct cagcacgggc gatatccgcg
     1381 atcgatcgca agatgatcgt ggtcgagaaa ggcaattcga gtggcaggcc cgaaacgcca
     1441 aacgcgccgc cagccgcccc ggctaaaacc gccaggctct tatgcatgcg gcgcctgtcc
     1501 ctgagtggct ttcccgcaag gcttttcatc gccacattga gactactccg gattgccgcc
     1561 tttgccgccc ggttcgccgt ttcagcaagc ggtgcgggga cgaaggacgc aagagcactc
     1621 gcctgccggc ctagaattgc gctgagccta atcgccaaac ttgagcgttc gagctcgctt
     1681 atggcccgtt gcagagctgc acgatcctca ggagaaagcc tggacgcacc gtccggaagg
     1741 acgtctacgc tagcgcgatc cggttgcacc tcacttgtcg ggaaattcat gcaatgcctc
     1801 atagaaaagg cgtccatatt gccgcttgcg taacaaaggc gcaatccaaa taaagcggcc
     1861 gcccgcagca tgactctcga aatctaaatc acggctgcgt taccagcctc agcaaagcaa
     1921 tacacaaatt tttaggttca tcgataggcc gcagggccga taccacgggc ttatgaagtg
     1981 ttcgcactcc tttcccaggc gtgttttgtc ggtcccaatt gtgatatcga actaaaatga
     2041 atatcattcc aaaggttttg atcgaaactg atgatggagg cttattttca aatatcacaa
     2101 ctctaattaa atcgtgaaat ttcgataaaa tcccacaact ttacttagag ccgccgcaaa
     2161 gcgagcgtgg cagaatttcc tttctcccat tgaggaaggg aaaaatatga aagttcggac
     2221 tgcgacatgg gctttggccc ccccgctgtt gtgggcgttt cttgcgggat tggtcgcgat
     2281 gacgccttct tgcgctgcga gcgccggcgc gccacgcccg gctgaaaggg cgtttatgcc
     2341 aattggcggc ccaacaagcg tcccctatgg gtgggtggat ttctgccgcc gctatcatgg
     2401 agaatgcgac ggcggtctca tcgtcccgcg cgacattaat ttgaccccac aaatgatgca
     2461 ggtaatcgat cgggtcaaca aatgggtcaa tgcttatgtc aaacccgtct cggacccaga
     2521 gcaatggggc gttattgacc aatgggacta cccatatgat cgaagaggcg actgcgagga
     2581 ttacgcactc ttcaagcgca aaatccttat tgaggagggg cttccgcgtc agggcttact
     2641 gatgacggtt gtcaaggacg aacaagacgt gggccacgcg atacttaccg tcaagacgaa
     2701 tagcggtgaa ttcgtccttg ataatttaaa tgacgaagta aagccctgga accggaccgg
     2761 ctatcgtttt gtgaaacgcc agtcgcaagc cgaccagaac ctttgggtgc aaataggcga
     2821 tccgaccacc acgcccgatt atgtctcgca ctgaggaaga ttttttagag cgtgtccagc
     2881 agaattggaa ccggtttgcg cccggacacg cagcaaaacg aagatgtaaa gaaaaccggc
     2941 gacaagggaa aagtcttcct gctccgggcg taggaccgcc aatcccgcgc aaagtacgga
     3001 cacatctggg tgcttgccgc gcaaccaaaa tgaatggcct ttgtgcgttg aatcttggcg
     3061 gagtctgacc ccgcacaact gatttaattt ggattttcct gcgcgtttgg tcctttcaac
     3121 tgccgccagc gtcaatgacc ttcgctcgct ggagcgctgg ccgggcaagg caacggtcca
     3181 cgtaagcgcg caatgccggc cgcgcgtaga caagtttgaa tacatcaatt ccaaagtaaa
     3241 gatctgtgcc tatcatgaca tcggccgctg aaaaccgctc tccaagcaga aaagggccgg
     3301 cggcaacagc ctcttcgagc acgtcgaaga cgcggtcata gtcaccaaat cctgccgact
     3361 caggcctcgg agaaatcttg taagccttcg ccataaacgc ttgctcgata catcccggcg
     3421 aaaagaaaag ccattgtaaa tagcgcccgc gcgcagaatc gccgggcggg ggcgcgagac
     3481 gcgcttcggg gcaggcctct gcgacatagg cgcagatggc gccggactcg gcgacggcaa
     3541 taggcccgtc ggtcagcgcg ggaacttttg ccataggatt gatcgcacaa aattccaccc
     3601 tcttttggtc gcctgacgaa aggtcaagct ggatgcgttc atagggaacg ccggcctctt
     3661 cgagagccca tagggcgcgc agcgagcgcg tgcgcggcgc ccaatagagt tgcatcattt
     3721 tcgtcttcct ccggttggat aactcaaatg tgctcgaccg caacgatccc tggaatcgcc
     3781 ttgagcgccc ctactgcttg cggagagaca taatacttcc caggcaactt cacttcgacc
     3841 tcgccgctac tcgtgaggag aatgagcaac acttcgccct cccctctttg acgcagctgc
     3901 cccacaattt cacccagcgg attcttgtcg tgcaacacaa tgcgaagtcc ctttccacag
     3961 cggctcgcag ctcgatccaa aggctcggca gaaacaatgc gagcccgcac ctcctcgcct
     4021 tcaagcgaag cttgcaagcc aagaagcacg gagatgcctt tctccaaaac ttcgcggtac
     4081 tgattaagtc tctcctgaaa caaaatcgct tcataatggc cggattggtc ggacagctgg
     4141 aggattccca ttttcgtgcc tgacttggtc cgccgctcat gccggtcaag caccgttgcg
     4201 gcgagccgcc cagtcgtagc cccttccttg accgcagcct cgaactcggc ccagcgctgc
     4261 acccgaagcc gggcgagcac cgtggcatag gcgtcgaggg gatgcccgga gagaaaaaac
     4321 cctacggact caaattcccg ccgcaacctt tctgcgagcg gccaaggctc ggttgtcggc
     4381 agtataatcg catcgagatt ttcgccgccg aacaattggc tttgcccggc gagcttctct
     4441 tcctcctgcc ggttcgcgaa cgcgagaatg gtttcggcgc ctgcataaac ccgcgcgcgg
     4501 ttggactcca gctcatcaaa agcgcccgct gcggcgagac tttcgatggt gcgcttgttg
     4561 acctcggccg attgcagcct tgcggcaaag tcagtcagac cccg
//
LOCUS       DATMPU010000167         2614 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_3236427,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000167 DATMPU010000000
VERSION     DATMPU010000167.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 2614)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2614)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2614
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3236427"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..705
                     /locus_tag="VNY06_03835"
     CDS             <1..705
                     /locus_tag="VNY06_03835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX13988.1"
                     /translation="AEQPSNVSTAPAEAPDLGPLEARLTALEKKLAQTESEATAPKTS
                     RRAEPDQENASAKEATRAEKIAIVAENLLRKLDHGEEFSADLSTLEDLGIPQAALAPL
                     RAASGSVVSTIRELTAQFAALSGKIIAAESTAQAGPDESFLDRLTRNAKSLVDVRRVS
                     ESSAADVQSLVTRIETALADHDVEVAYKAWTQLPSFARNVAQSWGEAAKARLDALNAA
                     KSIEADAVAVLAKPKR"
     gene            748..2274
                     /locus_tag="VNY06_03840"
     CDS             748..2274
                     /locus_tag="VNY06_03840"
                     /inference="COORDINATES: protein motif:HMM:NF018875.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme biosynthesis HemY N-terminal
                     domain-containing protein"
                     /protein_id="HWX13989.1"
                     /translation="MLRVLVYLIFFIALAFGLGWLIDRPGEVVLNWQGYRIKTSLLVG
                     LGVILSVVALLVLAWSFLRTAVRSPSAISSAARARRREKGYAALSRGIHAVGIGDAQL
                     AARAAAQVQKYLPGEPLALLLRAEAAQLAGDHYVVESIFKEMTERDDTRLLGYRGLHA
                     QAHRRGEIESAHHYASAAHQIAALPWSAGVILDQRVAAKDWQGALTALEDNRNFIDKL
                     AVGRQRAVLTTALALEKEHASPVEALRLARIANKRAPDLVPATALAVRLLARRGAARK
                     AAKLIESAWPLAPHPDLATLYLDLRPGESHNERLARARALAKLAPRDPESRMMLAGAA
                     IAACDFKAAREAMRPLIEGDERPTTRMCLLMAEIEESEHGESGYIREWLARASRAPRD
                     ACWIADGVMSDQWMPASPVSGKVGAFVWKRPDERIGASVEPADAIFRPIAASSAQSTI
                     LIEKQQAIAIPPPEAERLTMPESTGGASALQPAKEEGPEAEPNSQKDEGKPEGLRQLS
                     "
     gene            2389..2464
                     /locus_tag="VNY06_03845"
     tRNA            2389..2464
                     /locus_tag="VNY06_03845"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2422..2424,aa:Thr,seq:cgt)
BASE COUNT          552 a          753 c          801 g          508 t
ORIGIN      
        1 gcagaacaac cgtcaaacgt ttccaccgca cccgcagagg ctcccgatct tgggccactc
       61 gaagcgcgtt tgacagctct agaaaaaaaa cttgcgcaaa cagaatcaga agccacagcg
      121 cccaagacga gccggcgcgc ggaaccagat caggaaaatg cgtccgcaaa agaagcgacc
      181 cgcgcggaga agatcgcgat cgttgccgaa aatcttttgc gcaagcttga tcatggcgaa
      241 gaattttcag ccgatctctc aacgcttgaa gatttgggaa ttcctcaggc tgcactcgca
      301 ccattgcggg ccgcctccgg gtccgtcgtt tcgactatcc gggagctaac ggcgcagttt
      361 gcagcccttt ctggaaaaat catcgcggcc gaatcaacgg cgcaagccgg gccggacgaa
      421 agttttcttg accggttaac ccggaacgcg aaaagcttgg tcgatgtccg ccgggtcagc
      481 gaaagttccg ccgccgatgt gcaaagcctg gtcacgcgga tcgagaccgc gctcgcggac
      541 cacgatgtgg aagttgcgta taaagcctgg acgcaattgc ccagcttcgc caggaatgtt
      601 gcgcaaagct ggggtgaagc cgcgaaagcg cggcttgacg cattgaatgc cgcgaaatcg
      661 atcgaggccg atgccgtggc ggtcttggcc aagccgaagc gttaggcggc caaaacctgt
      721 aacgtcaaag tcctcgccga gtaaaacatg ctccgggttc tcgtctattt gatttttttc
      781 attgcgctcg cgttcgggct tggctggctc attgaccggc caggcgaggt agtactgaac
      841 tggcaagggt atcgcataaa gacgtcgctc ttggttgggc taggcgtcat cctgtctgtg
      901 gtggccttgc tcgttctagc atggtccttt ctgcgcaccg ccgtacgctc accatcggca
      961 atatcgagtg cggcgcgcgc gcggcgccgc gagaaaggct atgccgcgct ctcaaggggc
     1021 atccatgccg tcggtatcgg cgatgcgcag ctcgcggcaa gggccgccgc ccaggtccag
     1081 aaatatttgc caggcgaacc cttggctctg ctcttgcggg cggaggccgc gcaacttgcg
     1141 ggtgatcact atgtggtcga atccatattt aaggaaatga ccgagcgcga cgacacacgg
     1201 ctccttggct atcgcgggct tcatgcccag gcccaccggc gtggcgagat cgagtctgcg
     1261 caccattatg ccagcgccgc gcatcaaatc gccgccttgc catggtctgc aggagtgatt
     1321 ctggatcagc gagtcgcggc gaaggactgg caaggcgcgc tcacggcgct ggaggacaac
     1381 cggaatttca tcgacaagct tgctgtagga cgccagcgcg ctgttttgac cactgcgctc
     1441 gcgttagaaa aggagcacgc ctccccggtt gaagctctcc gtcttgctcg gattgcgaac
     1501 aagcgcgcgc cggaccttgt cccagcgacc gcactcgccg tgcggctcct ggctcgcagg
     1561 ggcgccgcgc gcaaggcggc gaaactgatc gagtcggcat ggccgctcgc acctcatccg
     1621 gaccttgcga cgctttatct cgatctgcgt cccggcgagt cccacaacga gcggctggcg
     1681 agggcgcgtg cgctcgcgaa actcgcgcca cgcgatcctg aaagccggat gatgctagca
     1741 ggcgcagcaa ttgccgcttg cgactttaag gctgcaaggg aggctatgcg acctctgatc
     1801 gaaggtgatg agcggccgac cacccgtatg tgccttctca tggcggagat cgaagagagc
     1861 gagcacggcg aatcaggata tattcgtgag tggctggcgc gcgcatcgcg tgcgccacgt
     1921 gatgcgtgtt ggatcgccga tggcgtcatg tcggaccaat ggatgccggc ctcgccggtg
     1981 agcggcaaag tcggcgcctt tgtctggaag aggccggatg agcgtatagg tgccagcgtt
     2041 gaaccagcag atgccatttt taggccgatt gcagcttctt cggctcagtc aaccatcctc
     2101 atagagaagc agcaagcgat tgccattccg cccccggagg cggagcggct aactatgccg
     2161 gagtcaacag gcggcgcgag cgcgttgcag ccggctaagg aagaaggccc ggaggccgag
     2221 ccaaattctc agaaagacga aggaaagccc gaaggtttgc gacagctttc ctgaactttg
     2281 tccggcaaag ggggagcctg gcagcggagg caaaccggca ttgctgtggg ccctcgtctt
     2341 ggctatgaat gcttatcgac tattggtgct tccgcgcatc cgactttagc cgcaatagct
     2401 cagctggtag agcacgtcat tcgtaatgac ggggtcgggg gttcgaatcc ctcttgcggc
     2461 accaattaaa tcaaaggttt atagataata cttaagccct ttttgaagtc tgatttggtc
     2521 ctggacaaca ttttggacaa atcacgagaa tttcatgggc cgagcaaaat tgcccgccat
     2581 gaaggcgggc ccagagccgc ttgcaaacct tgcg
//
LOCUS       DATMPU010000168         7299 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_2936584,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000168 DATMPU010000000
VERSION     DATMPU010000168.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 7299)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7299)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7299
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2936584"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..302)
                     /locus_tag="VNY06_03850"
     CDS             complement(<1..302)
                     /locus_tag="VNY06_03850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012590161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HWX13990.1"
                     /translation="MARGDEESLRQAIVGACLAMTRNGINQGMAGNISVRWKSGLLIT
                     PSGIAYDALTADDIVFMNMDGTYDHRHAASSEWRFHRDILSELANVNAVVHAHPIYA"
     gene            complement(316..963)
                     /gene="eda"
                     /locus_tag="VNY06_03855"
     CDS             complement(316..963)
                     /gene="eda"
                     /locus_tag="VNY06_03855"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003543005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008675 -
                     2-dehydro-3-deoxy-phosphogluconate aldolase activity
                     [Evidence IEA];
                     GO_function: GO:0008700 - 4-hydroxy-2-oxoglutarate
                     aldolase activity [Evidence IEA];
                     GO_process: GO:0009084 - glutamine family amino acid
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009255 - Entner-Doudoroff pathway through
                     6-phosphogluconate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HWX13991.1"
                     /translation="MLYPHSERTLAVLRLAPVIPVLTVQSIEDAIAQAGALIAGGLTV
                     LEVTLRTPAAMAAVTTLAKEFPNATIGAGTVVQVEQIKTAAAAGAAFLVSPGMTPLLV
                     EAAVHSPVPFLPGAATVSEALALQERGFEALKFFPAEPAGGAKYLASLAAPLPNLIFC
                     PTGGIDAQKAQTYLALTNVACVGGSWMVAPGLLAVQDYPKIEQLAREASAFAHRL"
     gene            complement(994..2805)
                     /gene="edd"
                     /locus_tag="VNY06_03860"
     CDS             complement(994..2805)
                     /gene="edd"
                     /locus_tag="VNY06_03860"
                     /EC_number="4.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019404090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004456 - phosphogluconate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009255 - Entner-Doudoroff pathway through
                     6-phosphogluconate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydratase"
                     /protein_id="HWX13992.1"
                     /translation="MVKVALAEITSRIRARSQDSRKKYLARIEAMRRAGPRRGRLGCA
                     NLAHGFAACARHEKQALRSGAVANLAIVTAYNDMLSAHQPFERYPAIIKQAAREAGGV
                     AQVAGGVPAMCDGVTQGYAGMELSLFSRDVIAMSAAIALSHDMFDAAVFLGVCDKIVP
                     GLVIAAQAFGHLPAVFIPAGPMPSGLPNPEKAKVRELHAAGKAGREDLLEAESAAYHS
                     PGTCTFYGTANSNQMLMEVMGLHLPGSSFVNPNTPLRDALTTAATERALALTALGNDY
                     TPVASILDERAFVNAIVALHATGGSTNHFIHLIAMARAGGIVLTLDDFADLSKLVPLL
                     TRIYPNGTADVNHFTAAGGTGFLIRTLLSAGLLHKDATTIAGHTLEFHAKEPYLDGGK
                     LAFREAPQISRDETVLRPATNPFSPDGGLKVLDGNLGRAVIKVSAVAPEQRKVRAKAR
                     IFHAQEEVEEAFRAGELESDVVAVVSFQGAKAIGMPELHKLTPLLTSVMSRGHKVALV
                     TDGRMSGASGKVLAAIHVTPEAIDGGPIARLRDGDIIALDAEAGTLTVELPSSILQSR
                     PPATPDLEAHTHGYGRELFQFFRAHVNGTDLGASIFG"
     gene            complement(2853..4328)
                     /gene="zwf"
                     /locus_tag="VNY06_03865"
     CDS             complement(2853..4328)
                     /gene="zwf"
                     /locus_tag="VNY06_03865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009468703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HWX13993.1"
                     /translation="MASRILKVQPFDLVVFGSTGDLAYRKLYPALLHRYLDGQFTQPT
                     KIIGVSRQSLDRTAFRVKVGAMLKKFASGAASDETIKSFFEMIDFVALDFGSDVGWDK
                     LGNAFSSDPERVRAFYLATGPDFFGPIAKKLAAHGFVTPKARIIVEKPIGRDGASAAR
                     INDAIGAVFSEQQIFRIDHYLGKETVQNLMALRFANALFEPLWNSAHIDHVELTVAES
                     IGVESRAGYYEKAGALRDMVQNHLLQLLCLIAMEPPASLDADSVRDEKLKVLKALKPI
                     DASNAVAVTVRGQYRSGASEGGAVPGYAEEVGNELTRTETFVALKVEIENWRWAGTPF
                     YLRTGKRLPKRVSEVTVAFREIPHSIFPEEAGKITRTRLAIRLQPDEGIKLWLMLKEP
                     GAGGIRLQHVPLDMNFADVFSIYAPEAYERLLMDVIRGNATLFMRRDEVEAAWRWIDP
                     ILEAWSHLDEAPRPYFAGTWGPAAAIALVEREGRSWSEEFV"
     gene            4671..5909
                     /gene="argJ"
                     /locus_tag="VNY06_03870"
     CDS             4671..5909
                     /gene="argJ"
                     /locus_tag="VNY06_03870"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018407393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HWX13994.1"
                     /translation="MAAPSPFAPLSYPDLPEIAGVRFAVGTAGIRYKDRTDVMLALFD
                     KGTEVAGVFTKSKCASAPVEWCRARLKRGKARALVVNSGNANAFTGRNGAEAAKFTAA
                     LAARASGGPANEIFLASTGVIGEPLDASKFDGVLEKLAANAARGGLFEAAKAIMTTDT
                     FPKVATCTALLGGVEVTLSGMAKGAGMIAPDMATMLAFLFTDAPIAAPALQAMLSKSV
                     KASFNAITVDGDTSTSDTLMLFATCAAAKHGAPRISAANDRRLASFKQALDRILLDLA
                     HQVVKDGEGARKFVEVKVTGAVSASSAKKIAFSIANSPLVKTAIAGEDANWGRVVMAV
                     GKAGERAERDRLAIWFNDVRVAHKGQRDPTYDEAEVSRLMRAAEVKIRVDLGLGKGKE
                     KVWTCDLTEDYVAINGDYRS"
     gene            5906..6307
                     /locus_tag="VNY06_03875"
     CDS             5906..6307
                     /locus_tag="VNY06_03875"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012591513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(deoxy)nucleoside triphosphate
                     pyrophosphohydrolase"
                     /protein_id="HWX13995.1"
                     /translation="MKLLLVAAVALIDPDCRVLIAQRPEGKTLPDLWEFPGGKVEIDE
                     RPEQALIRELNEELGITVEEACLAPLTFASFAYPDLHLLMPLYICRRWKGFVAAREHK
                     ALKWVFPKELRTYPMPPADAPLIPALIDLLG"
BASE COUNT         1586 a         2144 c         2070 g         1499 t
ORIGIN      
        1 gcataaatgg gatgggcatg aaccaccgcg ttgacgtttg ccagctcgga caaaatatcg
       61 cgatgaaaac gccattcaga ggaggccgca tgacggtggt cgtaggttcc atccatgttc
      121 atgaagacga tatcgtcggc cgtcaacgca tcataagcaa taccggaagg cgtaatcagc
      181 aggcccgatt tccagcgcac cgatatattg ccggccatgc cctgattgat gccatttcgg
      241 gtcatggcaa gacaggcgcc gacaattgct tggcgcagac tttcttcgtc gcctctggcc
      301 attcgaagtg ctcctttaga ggcggtgggc gaacgcggac gcttcccgag cgagctgttc
      361 aatcttggga taatcttgca ccgcgagaag ccccggcgca accatccagg agccgccgac
      421 gcaagccaca ttggtgagcg caagataggt ttgggccttt tgcgcgtcga taccgccggt
      481 cgggcagaag atcaaattgg gcaacggtgc agcgagcgag gcgaggtact tagccccccc
      541 cgctggctcc gcaggaaaga attttagtgc ttcaaacccg cgctcttgaa gtgccagcgc
      601 ctcggatacg gttgccgcgc caggcagaaa tgggaccgga gaatgcaccg ccgcctcaac
      661 aagaagcggc gtcatgccag gactgacgag aaaggctgcg ccggcagcag cggcagtttt
      721 gatctgctct acctgcacga ccgtcccagc accgattgtc gcatttggaa attctttggc
      781 gagcgtcgtc accgcggcca tggcggctgg cgtgcgcaaa gtgacttcga gcacggtcag
      841 cccgccggcg atcaaagcac cggcctgggc gattgcgtcc tcgatgctct ggactgtgag
      901 cacgggaatg acaggcgcga ggcgaagcac cgccaaggtc ctctcgctat ggggatagag
      961 cactagggcg cctttccaaa tagcataaag gaattagcca aaaatgctcg ccccgagatc
     1021 agttccgttc acatgggcgc gaaagaattg gaacagctcg cgtccataac catgcgtatg
     1081 ggcctcgagg tcaggagtag ccggcgggcg gctctgcagg atcgaggacg gaagttctac
     1141 cgtcaatgtg cccgcttccg cgtcgagcgc gattatatcg ccatctcgca accgggcaat
     1201 ggggccgcca tcaatcgcct ccggtgtgac atgaatagcc gccaggactt tccccgatgc
     1261 tcccgacata cggccatcgg tgaccaacgc aaccttgtgg ccgcgcgaca tcaccgaagt
     1321 gagtagggga gtgagcttgt gcaactccgg catgccgatc gctttggctc cctgaaaact
     1381 aacaacggcc accacgtcgg attcgagttc gcccgcgcgg aaggcttcct ctacctcctc
     1441 ctgcgcgtga aaaatgcgcg cttttgcacg cactttgcgt tgttccggcg cgactgcgct
     1501 gacttttatc accgcgcggc cgagattgcc atcgagcact ttcaaaccgc cgtccgggct
     1561 gaatggattt gtcgccgggc gcaggaccgt ctcatcgcgg ctaatttggg gcgcctcgcg
     1621 aaaagcaagc ttaccaccat caagataagg ctccttcgca tgaaattcga gcgtatggcc
     1681 cgcgattgtc gttgcatcct tatgcagcag gccggcgctg agcagcgttc tgataagaaa
     1741 tcctgtcccg cccgcggcgg tgaaatggtt cacatccgcg gtgccattcg ggtagattcg
     1801 tgtcaacaaa ggaacaagtt tggataggtc ggcaaaatcg tcgagcgtca ggacaatgcc
     1861 gcccgcccga gccatggcga tgagatggat aaaatgattg gtcgagcccc ccgtcgcgtg
     1921 caaggcaacg atggcattca caaaggcgcg ctcgtcgaga atggaagcaa ccggcgtata
     1981 gtcgttgcct aatgcagtga gcgcgagagc acgctccgtg gctgcggtgg tcagcgcatc
     2041 gcgcaaaggc gtgttcggat taacgaagga gctgccgggc agatgcagtc ccatcacttc
     2101 catcagcatt tggtttgaat tcgcagtgcc ataaaaggtg caagtgccgg gtgaatgata
     2161 ggcggcggat tccgcctcga gcaaatcttc ccgcccagcc ttgcctgcgg catggagctc
     2221 acggaccttt gccttttcag ggttcggcag accagatggc atcggcccgg caggaataaa
     2281 gacggcaggc aaatgcccaa aggcctgcgc cgcgatgacc agccccggca cgatcttgtc
     2341 acagacgcca agaaacaccg cggcgtcgaa catatcatgg gacaatgcga tcgccgccga
     2401 catcgcgatg acatcacgcg aaaacagcga aagttccatc cccgcgtagc cttgggtcac
     2461 gccgtcgcac atggcgggca cgccgccagc cacttgagca actccgcctg cctcgcgcgc
     2521 cgcttgcttt atgatcgctg gataccgctc aaaaggctga tgagccgaga gcatgtcgtt
     2581 ataggcggtg acaatggcaa ggttggcaac ggcaccgctg cgcagcgctt gcttttcgtg
     2641 acgcgcgcaa gcggcaaagc catgcgcaag gttggcgcac ccaagtctcc cacggcgcgg
     2701 cccggcgcgg cgcatcgcct cgatgcgggc gagatatttt ttccgtgaat cctggcttcg
     2761 cgcgcggatg cggctcgtta tttctgcaag tgcaactttc accatgtccg gctccttagc
     2821 gcgccgcgtc agagaggaag cgagcgctca tctcaaacga attcttcgct ccagctccgg
     2881 ccctcgcgct cgacaagcgc aattgcggcg gcaggccccc atgtgccggc aaaataggga
     2941 cgcggcgcct cgtccaaatg gctccacgcc tcgaggatcg ggtcgatcca gcgccaggcc
     3001 gcttcaactt catcgcggcg catgaacaag gtcgcgttgc cgcggatcac gtccatcagc
     3061 agccgctcat aggcttcggg cgcgtagata ctgaacacat cggcaaaatt catgtcgagc
     3121 ggcacatgtt gcaaccttat tcctccggca cccggctcct tcagcatcag ccagagtttg
     3181 atgccttcat caggctgcag cctgatcgcc agcctcgtgc gggtaatttt cccggcttcc
     3241 tcagggaaga tcgaatgggg aatctcgcga aaggcgaccg tgacttctga aactcttttc
     3301 ggcaagcgct tgccggtacg cagataaaac ggtgtcccgg cccaacgcca attttcgatt
     3361 tccaccttga gtgcgacaaa ggtctctgtc ctcgtcaact cattgccgac ttcctcggcg
     3421 taacccggca ccgcacctcc ttcgcttgcg ccggaacgat attgcccgcg cacggtcact
     3481 gccaccgcgt tgctcgcatc gattggcttg agagccttca aaactttgag cttctcatcg
     3541 cgaaccgaat cggcatcgag cgaggccggc ggctccatgg cgataagaca cagcaattgc
     3601 agcaaatggt tctgcaccat gtcgcgcagc gcgcccgctt tctcataata gccggcccgg
     3661 ctctcgacgc cgattgactc ggcgaccgtg agctcgacat ggtcgatatg agcggaattc
     3721 cacaaaggtt caaaaagcgc gttggcgaac cgcagcgcca tcaaattctg caccgtttcc
     3781 ttgccgagat aatggtcgat gcggaaaatc tgttgctcgg aaaatactgc cccgatcgca
     3841 tcgttgatgc gcgcagccga ggcaccgtcc ctgccaatcg gcttttcgac gatgatgcgc
     3901 gcctttggag tcacaaatcc atgcgcggcg agttttttgg cgattgggcc gaaaaaatcc
     3961 gggcctgtcg cgagatagaa cgcgcgtacg cgctcgggat cggaactgaa cgcattgccg
     4021 agcttgtccc atcccacgtc cgaaccaaag tccagcgcaa caaagtcgat catttcaaaa
     4081 aagcttttga tcgtttcgtc acttgctgcc ccgctcgcga acttttttaa catggcgccg
     4141 actttgaccc tgaaggcggt tctgtccaag gattggcgcg acacgccgat gattttggtt
     4201 ggctgcgtga attgcccatc aagatagcgg tgcaagagcg caggatagag ttttcgatag
     4261 gcgagatcgc ccgtgctacc gaagacgacg agatcgaagg gctgtacttt taggatacgt
     4321 gaagccatcg ctttccttgg caatttgacc gtttgagtgc agcatagcca agcggtgatc
     4381 ataagcaaga tggctgcgaa cagaattcga ccaaccttcg gcccgcgttc caatcgaatg
     4441 accgagcttg aagaaagatt tgatcgcggc ttcgacttga agacccgcgc caaatcctca
     4501 tgaagcccag caaggtcgta acttctcaat gctccaacat tcggctgcgg ggaatttggc
     4561 agtccggaat ttatcaaaaa ctgtgcccca cctggttgac gccattgatc ccatttccgt
     4621 aaaccgacgc aaattcctac aagaatcgct tgccccaagg agcaaggttc atggccgccc
     4681 cctccccttt cgcgccactt tcctatccag atttgcctga gattgcaggt gtacgttttg
     4741 ccgtgggcac cgcgggtatt cgctacaaag accggaccga tgtaatgctc gccctgttcg
     4801 ataaaggcac agaagtcgca ggcgtcttca ccaaatcgaa atgcgcctcc gcgccggttg
     4861 agtggtgccg tgcaaggctg aaacgcggca aggcgcgcgc actggttgtc aattccggca
     4921 atgccaatgc ttttacgggc cgcaatggag ccgaggccgc caaattcacc gccgcgcttg
     4981 ctgcgcgggc gagtggcggg ccagcaaacg agattttcct tgcctcgacg ggcgtcatcg
     5041 gcgagccgct tgatgcttcg aaattcgacg gcgttttgga gaagcttgcg gcgaatgcgg
     5101 cgcgaggtgg cctgttcgag gccgccaagg cgatcatgac gaccgacact tttcccaaag
     5161 ttgcgacctg tacggcgctg ctcggcggcg tggaggtgac gctttccggc atggccaagg
     5221 gagctgggat gatcgcgccg gatatggcga ccatgcttgc ttttctattc acggatgcgc
     5281 ccattgccgc gcccgccctg caggcgatgc tttcgaagag cgttaaagca tcttttaatg
     5341 cgatcactgt tgacggcgac acttcaactt ccgacacatt gatgctgttt gcgacttgtg
     5401 ctgccgccaa acacggcgcg ccaagaattt ctgctgcaaa cgaccgccgc cttgcttcgt
     5461 tcaaacaagc gctcgatcgc atcctcctcg accttgccca tcaggtcgtg aaagacggcg
     5521 agggcgcgcg caaattcgtc gaggtgaaag tgaccggcgc ggtctcggcg tcgtcggcaa
     5581 aaaaaatcgc tttctcgatt gccaattctc cgcttgtgaa aacggcgatt gcaggcgagg
     5641 acgccaattg gggccgcgtc gttatggcgg tcggcaaggc cggcgagcgt gccgagcgcg
     5701 acagactcgc gatctggttc aacgatgtgc gcgttgcaca taagggccag cgcgacccca
     5761 cctatgacga ggctgaagtc tccagactca tgcgcgcggc ggaagttaag atccgcgtag
     5821 atttgggttt gggaaagggc aaagaaaaag tttggacctg cgatctcacc gaggactatg
     5881 ttgcgatcaa cggtgattac cgctcgtgaa actattgctc gtcgccgccg tcgctctgat
     5941 agacccggat tgtcgcgtac tgatcgcgca acgtccagaa ggaaaaaccc tcccagatct
     6001 ttgggaattt ccgggtggca aggttgaaat tgatgaacgg ccggagcagg ccctcatccg
     6061 ggaactcaat gaggagctcg ggatcacggt cgaagaggct tgccttgcgc cgctcacttt
     6121 cgcgagcttc gcctaccctg acctccatct cttgatgcca ctctatatat gccgccgctg
     6181 gaaaggtttt gtggcggcgc gggaacacaa agcactgaaa tgggtttttc caaaggagct
     6241 tcgtacctat cccatgccgc cggccgacgc gccgctgatc ccggcgctta tcgacctatt
     6301 gggctgagaa ggcgtcaccc ttttttgcac cgcatccccc gggagcgtag ctcgcccctg
     6361 catccgctcc cgacctgccc cgggcacgcg agacgatttc gcgcgagcgg ggctcatgtc
     6421 tggttataga gagtggccgg ctataatgac gtttccgttg gcatgcactc aaggcgttcc
     6481 tcgccttcaa tagctcccat cctcacttat agtcgaaaag ctctgcgtcg ttccaggtgc
     6541 cgttgctgtt agtgacatac acaccagccc agtttccggt tccacacgcg ggttctatta
     6601 gaccctgaaa atgcgaatca ctcgtaaact aggagccccc ctctgtggat agaaatacaa
     6661 gtagaaggtc gaactagaat aaaatgccaa acaatattgt atatggaaga aatgccagcc
     6721 agtagcaact gctgctcctc cgtttactat tgtaccctcg gcagtttgag cgctcacttg
     6781 ggtgggggta gggggcgtgc gcgctccgct ggacgctgcc ggggaccgcg tttgggctac
     6841 cgcaaccccc agcgagccaa tgagtgtcat cgtcgccaaa aacgaaagct tggtaatcat
     6901 tcttccctca caatcctcat agaaaataga caattactaa ctatcatcaa aacatattta
     6961 atcaaagcca aaaacacaac gagaaccgct tcctccgcga cccgtattca aaggatgcgg
     7021 cgaaaattta attaccttgc acggttcccg aaaagcgcgc gtaattgcgc ttcgcattgc
     7081 gcagcgcgat gacggcgaga acacactcgc gctgagccgg atggcgtcac cggcgatctt
     7141 tgaccaggcg gcttttctat tttcttggtt gtcttcgtta tgcgaaggat atgccgaagc
     7201 ctattgctgt cgcggctcaa tgaatggacg gcccggaatt aatgcgggcc ggcagccggc
     7261 ggcaatagag gacgccaaag gtccgagagc agactgatg
//
LOCUS       DATMPU010000169         6196 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_4165775,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000169 DATMPU010000000
VERSION     DATMPU010000169.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 6196)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6196)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6196
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4165775"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..155)
                     /locus_tag="VNY06_03880"
                     /pseudo
     CDS             complement(1..155)
                     /locus_tag="VNY06_03880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012455796.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(226..1095)
                     /locus_tag="VNY06_03885"
     CDS             complement(226..1095)
                     /locus_tag="VNY06_03885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020179303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c1"
                     /protein_id="HWX13996.1"
                     /translation="MKKANAFGGGVGLAAVALFAVALVLNAASVSAETGAGQNPHEQP
                     KPPPQEWSFAGPFGHYNQPQLQRGFQIYREVCANCHSLKLIAFRNLADPGGPSFSEKQ
                     VKALAATYKIKDGPNDSGEMFERAGRPSDYFPWNFANEQAARAALGAVPPDLSLIAKA
                     RTYERGFPQFLIDPFIQYEEHGPDYIYALLNGYTNEQDPQWNEYMPGHKIAMPKPLAD
                     GVVDYEDGSPKTVPQYARDVTAFLMWASEPKLEERKRLGMSVLGFLSIYALLLFAAKK
                     KIWSHAGAKIPMD"
     gene            complement(1139..2404)
                     /locus_tag="VNY06_03890"
     CDS             complement(1139..2404)
                     /locus_tag="VNY06_03890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020174027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b N-terminal domain-containing
                     protein"
                     /protein_id="HWX13997.1"
                     /translation="MSGEQTYIPKGRLAQWFEKRLPILGFMHSSFVAFPNPRNLNYWW
                     TFGGILSFMLVAQIATGVVLVMHYTPHSTLAFASVEHIMRDVNYGWLLRYAHANGASM
                     FFLALYIHIFRGMYYGSYKEPREVLWLLGVIIYLLAIATAFLGYVLPWGQMSFWGATV
                     ITSLFSAIPKIGDSITTWLWGGYAVDNATLNRFFALHYLLPFMIAGVVVLHIWALHVP
                     GSNNPAGIEKKSEKDTLPFHPYFTVKDGFALVCFLVFYGWFVFYTPNYLLAADNYIEA
                     NPLQTPVHIVPEWYYLPFYAILRSIPSKLIGTAAMFAAVLIIAFLPWLDTSKVKSATY
                     RPLYRIAFWLFVICAVLLGYLGSKPPEGAYVIAARALTFYYFMHFLVFLPLLGIYERC
                     KPIPASIYDAVLEKPGRPAKTESDPMQAR"
     gene            complement(2483..3007)
                     /gene="petA"
                     /locus_tag="VNY06_03895"
     CDS             complement(2483..3007)
                     /gene="petA"
                     /locus_tag="VNY06_03895"
                     /EC_number="7.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016839713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045275 - respiratory chain complex III
                     [Evidence IEA];
                     GO_function: GO:0008121 - ubiquinol-cytochrome-c reductase
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol-cytochrome c reductase iron-sulfur
                     subunit"
                     /protein_id="HWX13998.1"
                     /translation="MAASTVTEEPTRRDFLFVATGAMGAVAAGFAAWPLIDQMSPDAT
                     TLAAASIDIDIANIAVGQIVTVKWRGKPVFISHRTQREIEAARGVDLNELRDPESDAA
                     RVQKPEWLIVIGVCTHLGCVPISNDGPIEGGYFCPCHGSVYDASGRIRQGPAPANLAL
                     PPYKFVSDTKITIG"
     gene            3180..3665
                     /locus_tag="VNY06_03900"
     CDS             3180..3665
                     /locus_tag="VNY06_03900"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011085271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008175 - tRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (cytidine(34)-2'-O)-methyltransferase"
                     /protein_id="HWX13999.1"
                     /translation="MHPNSATSLIVALYQPDIPQNTGTILRMCAYLGLPAAIIEPAGF
                     SASDRHFRRAGMDYLAHVDIARYPHWEAFEDWRRANGRRLVLLTTKASRPYTAFRYLP
                     GDILLAGRESAGVPEEVHDAADARLIIPLQPPMRSLNIAVALAMVAGEAVRQIRLRGF
                     S"
     gene            4795..5415
                     /locus_tag="VNY06_03905"
     CDS             4795..5415
                     /locus_tag="VNY06_03905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX14000.1"
                     /translation="MSTSLDYAKKLSDFLKSLMMISLFSMLFMIFCTHFRELDNICES
                     LLLEAPSINLEHVKFSAFGAEVEGSFSKQQIASQLEMEGVDDPETQEKVHEAVLGLKP
                     DEADRLMYVGNLQGTCEFARPSPKTKRYIEIDHQLKKKGLVAIKPDQLTYELEKKHGE
                     YLDQGFPISCYQMKLTDQGFNVKTALVKTLGRMFESNTTNPRLAMK"
     gene            5568..>6196
                     /locus_tag="VNY06_03910"
     CDS             5568..>6196
                     /locus_tag="VNY06_03910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020174023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="coproporphyrinogen III oxidase"
                     /protein_id="HWX14001.1"
                     /translation="MNEIDSADRCKSKTRAWFEQLQEQIIEAAERLEVEAEGPFAEAG
                     MMPGRFATKVWKRNEPDGREGGGGHMALLSGRVFEKMAVHSSTVFGSFPPGFAAQIPG
                     AEADPRFWASGISLIAHPWNPNVPTVHMNTRFVETTKAWFGGGADLTPMLDRRRQQTD
                     PDTRDFHASMKHACDSHSKVADHEKFKESCDEYFYIKHRNEPRGTGGIF"
BASE COUNT         1479 a         1709 c         1679 g         1329 t
ORIGIN      
        1 gatcacaccc gcgacagccg cttttcaaca cgtgcccaac gccgccgatg acgcgacggt
       61 cgtccccccg ctcaaccccg caaacatccg tcggcaagca aggctcaaat ccgcttccac
      121 tgctcatcgc tcggccaaaa caaattcctg ctcacaattt caattccttc ccgccaaaca
      181 atcactatct tattgtcagc gcagaaagac ttatgagccc tgaccctagt ccattggaat
      241 tttcgccccg gcgtgggacc aaatcttttt cttcgcggca aagaggagca aagcgtagat
      301 cgagagaaag cccaagacgg acattccaag ccgcttgcgt tcctcgagct ttggctcgga
      361 ggcccacatc aagaaggccg taacgtccct ggcatattgc ggaaccgttt tgggtgagcc
      421 gtcctcgtaa tcgacgacac cgtcggcgag gggcttcggc atggcgatct tgtgacccgg
      481 catatattca ttccattgcg gatcctgttc gttcgtatag ccgttgagga gcgcataaat
      541 gtaatcgggg ccgtgctctt cgtattgaat aaagggatca atcagaaact gggggaagcc
      601 acgctcataa gtgcgggcct tggcgatcaa agacaagtcg ggaggaacgg cgccaagtgc
      661 cgcccgtgcg gcctgttcat ttgcaaagtt ccatgggaaa taatccgatg gcctgccggc
      721 gcgctcgaac atttcgccag aatcgtttgg accatctttg atcttgtaag tggcggcgag
      781 tgctttcact tgcttttccg aaaagctagg cccgccggga tcagccaaat tgcggaaagc
      841 aatgagtttt aacgaatggc aattggcaca tacttcgcga tagatctgaa aaccgcgctg
      901 aagttgcggc tgattataat ggccaaacgg ccctgcgaaa ctccattcct gaggtggggg
      961 cttgggctgc tcatgcgggt tctggcctgc gccggtctcg gcagatacgc ttgctgcgtt
     1021 caaaaccaga gccaccgcga aaagcgcgac ggcagccagc ccgactccgc ctccgaatgc
     1081 gtttgctttt ttcattgcat ctttcctcgc ggcaagggta agccggtcac tgaccatgct
     1141 agcgcgcttg catcgggtcg ctttcggtct tcgcaggtcg gccaggcttc tccagcacgg
     1201 cgtcataaat cgaagctggg atgggtttgc acctctcgta aattccaagt aatggcagga
     1261 acacgagaaa atgcataaaa taatagaacg ttagcgctcg ggccgcgatc acataggcgc
     1321 cctccggcgg tttcgatccg aggtagccga gcagaactgc gcaaatgaca aaaagccaaa
     1381 aggctatcct gtaaagcggc cggtacgtcg ccgatttgac tttggacgtg tcgagccaag
     1441 gtaggaaggc gatgatcagc accgcagcga acatcgcggc cgtgcctatg agtttcgacg
     1501 ggatggaccg caaaattgcg tagaagggga gataatacca ttccggcacg atatggactg
     1561 gagtctgcaa tggatttgct tcaatgtaat tgtctgcggc aagcagataa tttggcgtgt
     1621 aaaacacaaa ccatccataa aacacaagga agcatacgag agcaaaacca tctttcaccg
     1681 tgaaatacgg gtgaaaaggc aaggtgtcct tctcagattt cttctcaatt ccagcagggt
     1741 tgttggatcc cggaacatgc aacgcccaaa tgtgcaggac aacaacacca gcaatcatga
     1801 agggtaacaa gtaatgaaga gcgaagaaac ggttgagtgt cgcgttgtcg actgcgtaac
     1861 cgccccaaag ccaggtcgta atcgaatcgc cgatttttgg aatcgccgag aaaaggcttg
     1921 tgataaccgt cgctccccaa aagctcatct gcccccaagg caacacatag ccaagaaatg
     1981 cagtcgcgat cgccagaaga tagattatca cgccaagcag ccatagaacc tctcgtggct
     2041 ctttgtagga gccgtaatac atgcctctga aaatatgaat gtaaagcgcg aggaagaaca
     2101 tcgaggcgcc attggcatgc gcgtagcgca gcagccagcc gtaatttacg tcgcgcatga
     2161 tgtgttcgac ggaagcaaaa gcgagcgtgg aatgcggcgt gtaatgcatg accagaacca
     2221 cgcccgtggc aatttgagcc accagcatga acgaaaggat tccgccaaaa gtccaccaat
     2281 agttgaggtt gcgcgggttc ggaaaggcca cgaaggagga atgcataaag ccgagaatag
     2341 gcaggcgctt ttcaaaccac tgtgccaggc ggcctttcgg tatatatgtc tgctctccgc
     2401 tcatgatctg ctcttcttat gatattcttc ccgaatcctc gaaggtgcag gctcgcgagc
     2461 aaggttttgc atgccgcgcg aactacccaa ttgtgatttt cgtgtcgctt acaaatttat
     2521 agggaggcaa agcaaggttg gcgggtgccg ggccctgccg gatgcggccg gacgcatcat
     2581 agacagaccc gtgacaggga caaaagtaac caccttcaat aggcccgtcg ttagaaatcg
     2641 gcacgcagcc gagatgggtg caaactccga tgacaatgag ccattccggc ttttgtaccc
     2701 gcgctgcgtc gctttccgga tcgcgcaact cattcaaatc aacgccgcgc gcggcttcga
     2761 tctccctttg cgtgcgatgg ctgatgaaaa caggctttcc gcgccatttg accgtgacaa
     2821 tctgtcctac agcgatattt gcgatgtcaa tgtcgatcga cgccgcagcc aaagttgtcg
     2881 cgtcaggact catttgatca atgagcggcc aggccgcaaa gcccgccgcg acggctccca
     2941 ttgcgcctgt ggcgacgaat agaaagtccc tgcgggttgg ctcttccgtt accgtgcttg
     3001 ctgccattat atcctcgaac cgccgtctgc ccccaatatc gaataatcga gccctggcat
     3061 ggaagcaata gccatgaggg ggcaatacca gcggcgcatt tgacgggtga agtcacgggc
     3121 gcgtgactgt tctaatggtc aataccgaag gaccgcggtg cgaatgcgct tttgcaacca
     3181 tgcatcctaa ttcagcgacc tcccttatcg tcgcgctcta tcagccagat attccgcaaa
     3241 ataccggcac gatcttgcgc atgtgcgctt atcttgggct ccctgctgcg atcattgagc
     3301 ccgccggttt ttcggcaagc gacaggcatt tccggcgcgc aggcatggat tatctcgccc
     3361 atgtcgatat cgctcgctat ccccattggg aggctttcga ggactggcgc cgcgccaacg
     3421 ggcggcggct cgtgttgctg acaaccaagg cgagccgtcc ctatacggca tttcgctatc
     3481 tccccggaga tattttgctg gctgggcgcg aatcagccgg cgtgcccgaa gaagttcatg
     3541 acgcagccga cgcgcgtctt atcatccctt tgcagccgcc aatgaggtcg ctaaacattg
     3601 ccgttgcctt ggccatggtg gcgggggaag cggtgcggca aattcgcctg aggggatttt
     3661 cctaaacgta aaagtctgaa tttgatttca gccactgccg cctgaaagcg caacgcgcca
     3721 ttcatctcat ggcggagagt ttggggagcg gctggattct gtcaaggacg agagcttccc
     3781 tcgcgccggc aaagccggtg ctccgcatcc ttgacagaat cgagcccgcc tcgccgataa
     3841 tgctcgccgc gcgatgaatg gcgaaatctg acattgaccg atggatcaaa tccagacttt
     3901 tgcatcttaa cggggggcga tctcgcggga ccatgctgac cgggcgggct ttatgggcca
     3961 tgttcaaact gaggcgaaat tcgccgccgc aactatttct agtggcgcgg tcttcggaag
     4021 agcccgcacg gatcgctgcc cggcttaggc cttctcgtta tgcaacacgg cacgagcgcc
     4081 gaggcccggc ctcctcaact tccgcctcac ctcttgaaga caaagtaggg tccggcataa
     4141 cctgaattta cctgggtgga ttcaaacgag ttcgccaact taaagccgtc ggacagcaga
     4201 gccgcaacac actgtgaaca agacgaagtc gcacccgcag cacacgacgc cggtgtcgta
     4261 acccccgcgc tagcactgga cagtcacaat gtccaccgcc atcgcctggc ctggcagccc
     4321 tgccgctcca aacaccgaaa aagccgcaag tttcttcatt gcctctctcc cttagctcaa
     4381 tttttggcca gtttagacaa tctgaagatt tggatttcaa aagacatcct ccagatgacc
     4441 aggactggcc tcgtccgagc ggttcgcctt ggccgttttg ggaaagctaa gtcttcgcca
     4501 ctctttataa cgaacgctcc cccccgttag gttccgcagc gtgccgaaga atttcggact
     4561 cgttgtgatc gggacgcacc tctcccttgg gtttcgctcg gcgcgccgcc atgacactca
     4621 cggacgattg ctacaacgtc gaggtgcttg ttaagagttt gagcgggacg ttacacgatg
     4681 ggcagaaggc cgagccgtga agggtgggca gaaaacgcgc ccaattgccc gggagcccct
     4741 ctcccatccg tcaggcttgt tatctatcct gattccatca tggaggagga tttaatgtcc
     4801 acgtctctgg attacgcaaa aaaattatcc gacttcctaa aaagtctgat gatgatcagc
     4861 cttttttcga tgcttttcat gatcttttgc acgcatttcc gcgagctcga caacatttgc
     4921 gaatcgcttc ttttggaggc tccgtccatc aaccttgagc atgtcaaatt ttcagccttt
     4981 ggggccgagg tcgagggatc tttcagcaag caacaaatcg cctcgcagct cgaaatggag
     5041 ggagtggacg atcccgagac ccaagaaaag gtgcacgaag ccgttcttgg gcttaagccg
     5101 gatgaggccg accggctcat gtatgtcggc aatttgcagg ggacttgcga gttcgcgagg
     5161 ccatctccta agacgaaacg ctatatcgaa atcgatcatc aattaaaaaa gaagggtctc
     5221 gtagccatca aaccggatca gttgacttat gaacttgaga agaaacatgg cgaatacttg
     5281 gatcagggct tccctatatc ctgttatcaa atgaaactca ccgaccaagg gtttaatgta
     5341 aagacggcac tggttaaaac tttgggaagg atgttcgaga gcaacacaac caatccacgc
     5401 ctcgccatga agtgagaaat agacaactgt agctcgtttg caacgtgcca ggcgggcgca
     5461 gattgatttg tgtcaatccg gcgagataaa gggcggttta tttgatggcc gcgccccgct
     5521 ttgccagaca agcgcggtta gtgggtctga cctcggaaga taatggcgtg aacgagattg
     5581 attccgcgga tcggtgcaaa agcaaaaccc gcgcttggtt cgagcaactg caggaacaaa
     5641 ttatcgaggc tgccgaacgt ctagaggtgg aagccgaagg acctttcgcc gaagcgggca
     5701 tgatgcctgg ccgtttcgca acaaaagtct ggaagaggaa cgagccggac ggcagagagg
     5761 gcggcggggg ccatatggca ctcctttctg gccgcgtgtt cgagaaaatg gcggttcact
     5821 cctcgacggt ctttggaagt tttccgcccg gattcgcggc gcaaattcca ggcgccgaag
     5881 ccgatccgcg attttgggcc tctgggatct cgttgatcgc gcatccctgg aacccaaatg
     5941 tcccgactgt gcatatgaat acgcgatttg tagagaccac caaggcatgg ttcggaggcg
     6001 gtgccgatct cacgcccatg ctcgaccggc ggcgccagca gaccgacccg gacacgcgcg
     6061 acttccatgc ctcaatgaag catgcctgcg acagccattc caaggtagcc gaccatgaga
     6121 aattcaaaga atcgtgcgac gagtatttct atataaagca ccgcaacgaa ccacgcggca
     6181 cgggcgggat ttttta
//
LOCUS       DATMPU010000170         3544 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_4165830,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000170 DATMPU010000000
VERSION     DATMPU010000170.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 3544)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3544)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3544
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4165830"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..226)
                     /locus_tag="VNY06_03915"
     CDS             complement(<1..226)
                     /locus_tag="VNY06_03915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX14002.1"
                     /translation="MDHAKVATPPPDEADALLEFDAPRDSSTWVRSLFSPVTTPRRAS
                     FGEMIRFNDGMNENHGQENRARIGTSDRSST"
     gene            339..605
                     /locus_tag="VNY06_03920"
                     /pseudo
     CDS             339..605
                     /locus_tag="VNY06_03920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004867878.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(638..1267)
                     /locus_tag="VNY06_03925"
     CDS             complement(638..1267)
                     /locus_tag="VNY06_03925"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018407978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HWX14003.1"
                     /translation="MLDNKHVKESYGRWAPIYDFVFTLVMRPGRKAAASAVGRIGGNV
                     LDVGVGTGLELPMFDKKTRLTGIDLSEPMLRRAKERVRARGLGNVDGFVVMDALALAF
                     PDGHFDAVVAPYVLTVIPEPRASLDEMARVVKTGGEIILVNHFGVGTGFMGWIESFLE
                     RRSAKLGWRPEFPWSILGDWLAATPSVRLAERRKLPPLGLFNLTRIKKL"
     gene            complement(1448..2689)
                     /gene="mnmA"
                     /locus_tag="VNY06_03930"
     CDS             complement(1448..2689)
                     /gene="mnmA"
                     /locus_tag="VNY06_03930"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019860759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HWX14004.1"
                     /translation="MNENIVPEETVIPGDLEATTLVPNKPAAATRVVVAMSGGVDSSV
                     VAALLKDRGYDVIGVTMQLYDHGAAVHRKGSCCAGQDIKDARLTAARLGIPHYVLDYE
                     ERFRRKVISPFAQSYAAGETPVPCIACNSAIKFADLLETAQDLGADVLATGHYVSSRL
                     DASGNRALYRAQDLARDQSYFLFATTKAQLNMLRFPLGGLEKAQVRALAKKFGLLVAD
                     KADSQDICFVPTGRYSDVIERLTPGAALPGDIVHIDGRLLGRHPGVIHYTVGQRRGLG
                     LGGLGAGEALYVVKLDASRAHVIVGPRQALETRRVHLRAVNWIGRGGLADQPQGAEIC
                     VQIRSSRSPVRGFLFASADDTTVEFAAAEDSVSPGQACVFYSLEHPPARVLGGGFIVS
                     ADRACQEFPENDFTKVQALPV"
     gene            2688..2981
                     /locus_tag="VNY06_03935"
     CDS             2688..2981
                     /locus_tag="VNY06_03935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX14005.1"
                     /translation="MAAIHRSINRPADKMFADLCAEITTGKIISAQMVLPHPKTVLRA
                     LLVPFRLANRLTGFSRVVAKACSHQRLFNTSPSENANLHSRAEPVPKSASSLT"
     gene            3160..3504
                     /locus_tag="VNY06_03940"
     CDS             3160..3504
                     /locus_tag="VNY06_03940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011156827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1153 domain-containing protein"
                     /protein_id="HWX14006.1"
                     /translation="MAEPNRIRAKYVIGPDGSPLTVADLPPSTTKRWVIRRKAEVVAA
                     VRGGLLSLDEACKRYTLTVDEFLSWQLSIDRHGLAGLRTTRIQQYRPDAEASRPIGHA
                     YAASVPSGPGRQ"
BASE COUNT          802 a         1033 c          953 g          756 t
ORIGIN      
        1 ttgttgagga acgatcggac gtgccaatcc gggcgcgatt ttcctgcccg tgattttcat
       61 tcataccatc attaaatctt atcatttcac cgaacgatgc cctccggggg gtcgtgacgg
      121 gtgaaaagag cgatcggacc caggtcgatg aatcgcgcgg ggcatcgaat tcgagcagcg
      181 cgtcggcctc gtcaggcggg ggcgtggcga ctttggcatg atccatgaaa ccaaaattta
      241 accggcgttg gttaacttgg agccgcctag tagtttgcgg aaaaactgct ggccacgcct
      301 tgtgagcggt gattcatcac gaatcggtgg ggggcaccat gcgcgggcga ttttgggacc
      361 aaggcggatt gttttcctac gcctgaggcg cgagtgtcag cgcgccatcc attgcgaaag
      421 attcgtgaac tggtccggga agttctgaaa gacctgagcc gaatctttgg gaaactctat
      481 gcgagcgaag gacgcccatc gatcccaccg gagcaattgc tgagcgcctt gctgctgcaa
      541 gtcttctacg gatcggctcg gaacggcagc tgatggaaca gctaaattac aatcttttgt
      601 gtcgttgatt cgtcgggctt tcgccggatg atccggtcta gagctttttg atacgagtca
      661 ggttaaaaag tcccaaaggc ggcaatttac gccgttccgc caggcgaacg gagggggtcg
      721 ctgccagcca atcgccaagg atcgaccaag gaaattccgg ccgccagccg agtttggcac
      781 tgcgtctctc aagaaatgac tcaatccaac ccataaaacc tgtacccacc ccaaaatgat
      841 tgacgagaat gatctctccg cccgttttca caacacgggc catttcatcg agactggcgc
      901 gcggttctgg gatcaccgtc agaacataag gcgcgaccac ggcatcgaaa tgcccgtccg
      961 gaaatgcaag ggccaacgcg tccattacca caaatccatc cacatttccc aaacccctgg
     1021 cgcgaacccg ctcctttgcg cgccgtaaca tcggctccga aagatcgata cccgtcagcc
     1081 tcgtcttttt atcgaacata ggcaattcga ggccggtgcc gacgccgacg tcgagcacat
     1141 ttccgccaat gcggccaacg gcggaagctg cggcctttcg tcccggcctc atcacgagag
     1201 tgaagacgaa atcatatatg ggcgcccacc gcccgtagga ttccttgaca tgcttgttgt
     1261 ctagcactgc ctgcctgccc aattgggcgg cggcaggggc caaggcaagc gtttcaaaat
     1321 ttcctcttgc acgagcgctg acttccgttg tgccgttgcg gcttcatttc atggaccacc
     1381 gccgaggtga attgtatgtt aacgtcatcg atacttttat ttgcgcagcc ctgtgctcgc
     1441 tcttttctca aaccggcaac gcttgaacct ttgtgaaatc attttccggg aactcctggc
     1501 aagcccgatc ggcagagaca atgaatccgc cgcccaaaac ccgcgcgggc gggtgctcaa
     1561 gtgaataaaa gacgcaagcc tgacccggtg aaaccgaatc ttccgccgcg gcaaattcga
     1621 cagtggtatc atcggcgctt gcgaacagaa atccccggac cggggaccga gaagagcgga
     1681 tctgcacgca aatctccgcg ccttgcggtt gatccgcgag cccgccccgc cctatccaat
     1741 tgacggcgcg caaatggacc cgacgcgttt ccagcgcctg acggggtcca acaataacgt
     1801 gggcccggga ggcatcgagt ttcacgacat aaagcgcttc cccggcaccc aatcctccaa
     1861 gcccgagacc tcgcctttgg ccaaccgtat aatgaatgac accaggatgg cgcccgagca
     1921 agcgcccatc tatatggaca atgtctccag gaagtgccgc accaggcgtc aagcgttcaa
     1981 tgacatcgct gtatcttccg gttggaacaa aacaaatatc ctgactatcc gctttatcgg
     2041 cgacgagcag accaaacttt ttagcgagcg cgcggacttg cgccttctcc aagccgccga
     2101 gcggaaatcg caacatgtta agctgcgcct tcgtcgtcgc aaacaaaaaa tagctttgat
     2161 cgcgcgcgag gtcctgcgcc cggtagagcg cgcgattgcc ggaggcatcc agccttgagg
     2221 aaacataatg gccggtggcc aaaacatcgg cgcccaagtc ttgcgctgtt tctaagagat
     2281 cggcgaattt gattgcgctg ttgcaagcaa tgcaaggcac cggcgtttcg cctgccgcat
     2341 agctctgtgc gaatggcgag atgaccttgc gccggaagcg ctcctcatag tccaaaacat
     2401 aatgtggaat gccgaggcgg gcggcggtca gacgcgcatc cttaatgtct tgcccggcgc
     2461 aacaggagcc cttgcggtgg accgccgcgc catgatcata aagttgcatc gtgacgccga
     2521 tgacatcata accccgatcc ttgagcagcg cggccacgac cgaagagtca acgccgcctg
     2581 acatcgcgac aaccacccgc gttgccgcgg cgggcttatt tggaacgagt gtcgttgctt
     2641 cgagatcgcc gggaataacc gtctcctccg gcacaatatt ttcattcatg gctgccattc
     2701 atcgctcgat caaccggccg gctgacaaaa tgttcgccga tctctgtgct gaaataacaa
     2761 caggcaagat tatttccgca caaatggtgt tgccgcatcc aaagacagtg ctgcgcgcct
     2821 tgcttgttcc attccgttta gcaaatcgtc ttacgggctt ctcgcgcgtt gttgcgaagg
     2881 cctgctcgca ccaacgctta tttaatacct cgccaagcga gaatgcaaat ctgcattcca
     2941 gggcggagcc tgtgccgaaa tcggcttctt ccttaacgta acgcaaatca atcgtctcaa
     3001 ttagagacat aatccgtgcc tctccacgac tgcagcaaat attcaaagtc attcgaatgg
     3061 aatttcccta gcaaaaccac tgattccgtt taggcaaatc ttaaaacacc ccgcttattt
     3121 tgactgtcat aggatctgtc tggtgtgtga gtcttgaaca tggccgaacc aaatcgtatc
     3181 cgcgcaaaat atgtcatcgg gccggacggg agtcctttga ctgtggccga tttgccgcct
     3241 tcgactacca agcgctgggt gatccggcgt aaggcagaag tcgtggcggc ggtgcgcgga
     3301 ggcctgttgt cgctcgacga ggcgtgcaag cgctatacgc tcactgtcga tgagttttta
     3361 tcatggcaat tgtccattga ccggcatggg ctcgctggac ttcggacgac ccgcattcag
     3421 caatataggc ctgatgccga agcgagcagg ccaataggtc acgcctatgc tgcgtccgtg
     3481 ccatcggggc cgggccggca atagagcgcc tcgattcgcc ggcgcgcgct caataatagt
     3541 ccgg
//
LOCUS       DATMPU010000171         8008 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_140123,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000171 DATMPU010000000
VERSION     DATMPU010000171.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 8008)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8008)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8008
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_140123"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            43..210
                     /locus_tag="VNY06_03945"
                     /pseudo
     CDS             43..210
                     /locus_tag="VNY06_03945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002715652.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
     gene            409..657
                     /locus_tag="VNY06_03950"
     CDS             409..657
                     /locus_tag="VNY06_03950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX14007.1"
                     /translation="MGQCRCGRSEDGPIPAVHGVVRWRLIDLAQWAFEEFRVSIFARV
                     RAWLATPIPSSEADTPTPISGLAQMGCVLANRVKLRKS"
     gene            884..3503
                     /gene="clpB"
                     /locus_tag="VNY06_03955"
                     /pseudo
     CDS             884..3503
                     /gene="clpB"
                     /locus_tag="VNY06_03955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016918884.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
     gene            3703..6168
                     /gene="gyrB"
                     /locus_tag="VNY06_03960"
     CDS             3703..6168
                     /gene="gyrB"
                     /locus_tag="VNY06_03960"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003610463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HWX14008.1"
                     /translation="MAEPLARPPLPLTPETEAYYGAESIKVLRGLDAVRKRPGMYIGD
                     TDDGSGLHHMVYEVVDNAIDEALAGYATSVSVTLNADGSVTVTDDGRGIPTDIHSEEG
                     VSAAEVIMTQLHAGGKFDQNSYKVSGGLHGVGVSVVNALSSWLNLRIWRDEKEHLLRF
                     EEGNATGPLVVSGPAPVGAGKPLRGTEVTFLPSPKTFSMVDFDHATIEHRLRELAFLN
                     SGVRVELTDVRGVEVKRDEFHYDGGLEAFVRYLDRVKTPLISNPIVVKGEREKIAVEA
                     ALWWNDSYHEHVLAFTNNIPQRDGGSHLAGLRAALTRQVTGYAERAGLTKREKVDLNG
                     DDCREGLTCVLSVKVQDPKFSSQTKDKLVSSEVRPAVEGLVNEMLGAWFEEHPQEARA
                     LVGKVVEAAQAREAARKARELTRRKGALDVANLPGKLADCQERDPAKAELFLVEGDSA
                     GGSAKQGRNREYQAVLPLRGKILNVERARFDKMLSSQEIGTLITALGTGIGPGEFNPD
                     KLRYHKVIIMTDADVDGAHIRTLLLTFFYRQMRELIERGHLYIAQPPLYKVRRGNSEQ
                     YLKDERAREDYLIGTGIEGAVLRLSSGEVRADSELRAIVEESRAVRHILAGLHSRYIR
                     HVVEQAAIEGALKPVVSENVAEALALAATIAGRLDVLAEDMERGWQGTVEDDGYLFTR
                     ELRGVRQAASLDAGLLASTEARRLNDHAQSLHDGFAGPALFLRRGEETQVFGPSDLLD
                     TVMAGGQKGITQMQRYKGLGEMNPDQLWKTTLDREARSLLQVKIKEADDAEDIFVKLM
                     GDVVEPRREFIQENALSVANLDV"
     gene            6446..6835
                     /locus_tag="VNY06_03965"
     CDS             6446..6835
                     /locus_tag="VNY06_03965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX14009.1"
                     /translation="MRDDVTFDDVSYGLQMRLYDLVKGKKIKGNVDNSGRFSLNPFSL
                     DVIVHSTGGPVVRNWLSLYLRDVCGGVFEKSPIRHFIMMAPANFGSRLAAQGKSTLAK
                     LFKGGFETGRKILEGLELGSPFLWDRS"
     gene            6768..7778
                     /locus_tag="VNY06_03970"
     CDS             6768..7778
                     /locus_tag="VNY06_03970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX14010.1"
                     /translation="MKQAVKSLKALSLVAHFYGTDRDLFGKGLYPSTNRGTFLYILSE
                     TDTYGRLKGLVAVGASENGSDGTIRAASASLDSSVKLEVNFIDPAHPQVNIITQSPVF
                     KLVKECNHSTIVPSPNDTAVHPAFKIIKQCLSIQNTTQYTTLRNTFDTENEQLYEENK
                     VYRKDNESVDPINRFQQFIVRVLDNMGNAVSDYRLDFHIVDNSIQRSTWTAQSPPPAL
                     DKYSDYNVRIIEKVISDVEQHSVEPSYRTFFINIDELNKLLGDLGSAHPNAYVSLNLD
                     ALPSAPGVSYNTDLIKYVGLNSSFNGKSFVKKNTSTLVEIRLQIQAFTISQRQLSQTS
                     AN"
BASE COUNT         1832 a         2155 c         2311 g         1710 t
ORIGIN      
        1 atcggatctc agacatgcga cagacgttga aatccactcg cgatcgtatt ttcatctctc
       61 ttatgcgtcg agcccttgga aaagcgcatc gcactcgtca tcggcaacgc caattactcg
      121 gcgaagccgc tcgcgacgcc agccaatgac gcaggtttga tcgcccaaac cttgcaggcc
      181 gctggtttcg atgtcgcggg cgcccgcgac caatcaatac gatccccact gcgatcgcgc
      241 cggagagcca atatctgata atagcatccc tttgaacggc tttgtcaccg ttcgtgggtt
      301 ttacttgtca atagggcgtg gcgctctctg cccacatgtc gttatttcaa tgccttagga
      361 tcctcttggc cgagccaagt tggctcgaat atcgcctata cctggcgaat gggacaatgc
      421 cgttgcggca gatcggagga tggccctatc ccagccgtcc atggcgtcgt gcgctggcgg
      481 cttatcgatc tggcgcaatg ggcgttcgag gagtttcgcg tctcgatctt cgcgcgggtg
      541 cgcgcttggc ttgccacgcc gatcccatcg agcgaggccg acacgccgac gccgatatcc
      601 ggactggcac agatgggatg cgtccttgcc aatagggtca aattgcgcaa atcatagcgc
      661 caatgtcggt catctgcgga atgtcggttg aggaaacgcc ggatgggctg cgagctttgc
      721 cgcgaaccgc accgccaaaa ccggaggtcg cgcgaagctg ccgtggtggc tgtcccgtat
      781 tttggctttc caaataaaga gcgcgaaatt cgggaggcct accaacgccg ttcgccacgg
      841 ccgctggcat gaggggtggc tttgcaaccg ggagatatcc gtcatgaata tcgagaaata
      901 taccgaaagg acgcgaggtt tcattcagag tgcgcaatcc ctcgccatcc gcgaaggaca
      961 ccagcaattt acgcccgaac atgttttgaa ggttcttctt gacgacgagg aggggctggc
     1021 cgcaggcctc atcgacaggg cgggggggcg ctcgcgcgac gcgctccgcg acacggaact
     1081 cgcgctcgcc aaattgccga aggtgcaagg cgccggcgcc gggcaactct atctcgcgcc
     1141 cgcgacggca cgcattttcg acaatgccga gaagattgcg caaagggcag gagactccta
     1201 tgtcacggtc gagcggctct tgcttgcgct tgccatggaa aaaagcacgg aagccggcaa
     1261 gattctggat cgggcgggcg tcacgccgca gactctcaac gcggcgatcg aggatctccg
     1321 caagggccgc accgccgaca atccaaccgc ggaaaatgcc tacgatgctt taaagaaata
     1381 tgcgcgcgat ctaaccgagg cggcgcgggc gggaaaactc gatccggtga ttggccgcga
     1441 tgaagaaatc cgtcgaacca ttcaggtatt gtcgcggcgc acgaagaaca atccggttct
     1501 gatcggcgag cccggagtcg gcaagaccgc gatcgtcgaa ggccttgcgc tgcgcattgt
     1561 aaacggcgat gtgccagaga accttcgcga caagaagctt cttgctctcg acttgggagc
     1621 gctcatcgca ggcgcgaaat atcgcggcga attcgaggaa aggttgaagg cggttttaaa
     1681 cgaaatcatc gccgccgcgg gcggcatcgt cctcttcatc gacgagatgc atacgctcgt
     1741 tggcgccggc aaggcggacg gcgcgatgga cgcatcgaat cttttgaaac ccgctctggc
     1801 gcgcggtgaa ttgcattgcg tcggggcaac gacgcttgac gaatatcgca agcatgtcga
     1861 aaaggatgcc gcgctggcgc gccgcttcca gccggtgttc atctccgagc cgacggtcga
     1921 ggacaccatc tcgattctgc gcggtctgaa ggaaaaatac gaattgcacc atggcgtgcg
     1981 gatcaccgat gccgcgatcg tcgccgccgc gaccttgtcg caccgctata tcgccgacag
     2041 atttctcccc gataaggcga tcgacctcat cgacgaagcg gcctcgcgcc tgcgcatgca
     2101 agtcgattca aagccggaag agctcgatga gctcgaccgc cgcatcatcc agcttaagat
     2161 cgagcaggaa gccctgaaaa aggagtcgga ttccgcctcg aaggagcggc tggagaagct
     2221 tagccacgaa ctcgccgagc ttgaggaaaa atccgcttcg ctggccgcgc gctggcttgc
     2281 ggagaaggac aagctcggca ccgaacagaa gctcaaggag cagctcgaag ccgcccgcaa
     2341 cgagcttact caggcccaac ggcgcggcga atatcagcgc gccggtgaac tcgcttatgg
     2401 cgtcattccc gatctcgaaa gaaagctcgc cgaaaccgag gcggaagggg gtggcgttct
     2461 cgtggaggaa gcggtgaccg ccaaccacgt cgcgcaggtc gtctcgcgct ggactggaat
     2521 tcctgtcgac aagattctgg agggcgaacg cgacaagctt ttgaaaatgg aggaagcgct
     2581 cggccagcgc gtcgtcggcc aggcagaggc ggtgaaggcg gtctcgaccg ccgtgcggcg
     2641 cgcgcgggcg ggtctccaag accccaatcg cccgattggg tccttcatgt ttttagggcc
     2701 aaccggcgtc ggcaagacgg agctcaccaa agcgctggct gcctttctgt tcgacgacga
     2761 aaccgcgctc atacgcatcg acatgtcgga atatatggag aaacattccg ttgcccggct
     2821 gattggcgcg ccgcccggct atgtcggcta tgaggagggc ggcgccttga cggaggcggt
     2881 gcgccgcagg ccctatcagg tcgttctctt cgacgagatc gagaaggcgc atcccgacgt
     2941 ctttaatgtc ctgctgcagg tgctcgacga tggccgtctg accgacgggc aggggcgcac
     3001 ggtcgatttc cgtaatgtct tgatcgtcat gacctccaat ctcggcgctg acttgctcgt
     3061 gctgcaaaag gagggcgagg attcctcggc cgtccactcg gaggtcatgc agatcgtccg
     3121 ggcgcatttc cggcccgagt ttttgaaccg tgtcgacgaa ataatcctgt ttcaccggct
     3181 gcgccgcgag gacatgagtg cgattgtcga tattcagttc gcgcggcttg cgaagcttct
     3241 ggaagatcgc aaaatcgtcc ttgagcttcg cccagaagcc cgcgcgtggc tcgccgacaa
     3301 gggctatgat ccagcctatg gcgcacggcc tttgaaacgg gtcatccaaa agagcgtgca
     3361 ggatcccttg gctgagaaga tctggagaaa ttcacgatgg cgagagggtt ttgatcgatg
     3421 ccaacgcagc agctgggctt ttaattggag gagaagcgtc cagccattcc cgcaggccgt
     3481 ctcaagccac aaacctacaa taggtgcggt cccattcggc gtgattcgaa ggcggatatt
     3541 aaacattacc ccgcatgggg ttagggaaat gtcgtaccac tttcccacag actttagtac
     3601 gaatccaatc acgcatcaat atgcatgacg tttgaatcct aaacggctat aattcgacac
     3661 ctcagttgag ccttttccaa tagcagaaag attgcattcc aaatggccga acccttagcc
     3721 aggccgcctc tccctttgac tcccgaaaca gaagcttact acggcgctga atcgatcaag
     3781 gtgctgcgcg gtctcgatgc cgtacgcaaa cgcccgggca tgtatattgg agatactgac
     3841 gatggctcgg gcctccatca catggtctat gaagttgtcg ataatgccat tgacgaggcc
     3901 ctggcagggt atgcgactag cgtttccgtg actctcaacg ccgacggctc ggttaccgtt
     3961 acggatgatg ggagaggaat tccaaccgat attcatagcg aggaaggtgt ctcggcggcg
     4021 gaggtcatca tgacccagct tcacgcaggc ggaaaattcg atcagaattc ttacaaagtg
     4081 tcgggtggcc tgcacggcgt tggcgtttca gtggtcaatg ctttgtcgag ctggctgaac
     4141 ctgcgaattt ggcgcgacga aaaggagcat ctccttcgtt tcgaagaagg taatgctact
     4201 ggaccgcttg tggtctcggg acctgcgccg gtcggcgcgg gcaagcccct gcgcgggacg
     4261 gaagtcacgt tcctgccctc gcccaagacc ttctccatgg ttgattttga tcatgcgaca
     4321 atcgaacacc gtctccgtga gctcgccttt ctgaattctg gcgtgcgcgt tgaacttact
     4381 gatgttcgcg gcgttgaggt caagcgggat gagtttcact atgatggcgg cctcgaagct
     4441 tttgtgcgct atcttgaccg cgtgaagacg ccgcttattt ccaatccgat agtagtcaag
     4501 ggcgagcggg aaaagatcgc ggtggaagca gctctttggt ggaatgattc ctaccatgag
     4561 cacgtcctcg catttactaa caatattccg cagcgtgatg gcggctcgca tcttgcgggc
     4621 ctgcgcgcgg cacttacccg gcaggtgaca ggctatgcgg aacgcgcagg tcttaccaaa
     4681 cgtgagaaag tcgatctcaa cggcgatgat tgccgcgagg gcctcacctg cgtgctttcg
     4741 gtaaaggttc aggacccaaa gttctcctcc caaaccaagg ataagctcgt gtcctccgag
     4801 gtgcggccag ccgtggaagg gcttgtgaat gaaatgcttg gggcttggtt cgaggagcat
     4861 ccgcaagaag cacgtgctct ggttggtaag gttgttgaag cggcgcaggc gcgtgaggcg
     4921 gctcgaaaag cacgagagct cactcgccgt aaaggggcgc ttgatgttgc caatctgccg
     4981 ggcaagctcg ccgattgcca ggagcgcgat ccagccaagg cggaattatt tctggtagag
     5041 ggcgactcgg ctggcggctc cgccaagcaa ggccgcaaca gggaatatca ggccgttctt
     5101 ccgctacgcg gaaagatcct aaatgtcgag cgcgcgcgct tcgataaaat gctatcgagc
     5161 caggagattg gcaccttgat taccgcgctc ggcacgggaa tcgggcctgg cgaattcaac
     5221 cccgataagt tgcgttatca caaggtcatc attatgacgg acgcagatgt cgatggcgcc
     5281 catatccgca cgcttctgct taccttcttt tatcggcaga tgcgtgagct gatcgagcgc
     5341 gggcatcttt atatcgcgca gccgccgctc tacaaagtaa ggcgcggcaa ctccgagcaa
     5401 tatttgaaag atgagcgcgc gcgcgaggat tatctgatcg gaactggtat tgagggcgcg
     5461 gtgctgcggc tttcgagcgg cgaggtgcgt gctgactctg aacttcgcgc gatcgtggaa
     5521 gaatcgagag ccgtacgcca cattctggct gggctccatt cgcgctatat tcgtcacgtc
     5581 gtagagcagg ccgcgataga gggcgcgttg aagcccgtcg tttcggaaaa cgtggccgag
     5641 gctttagcac ttgccgcaac cattgccggc cgtctcgacg tactcgcaga ggatatggag
     5701 cgtggctggc agggcacggt tgaagatgac ggttatctct tcacccgcga gctgcggggc
     5761 gtacgtcaag cggcaagcct cgacgcgggg ctattggcct ccacggaggc gcgtcgcctc
     5821 aatgatcacg cgcaatctct ccatgacggt ttcgccggac cggcgctgtt tcttcgcagg
     5881 ggcgaggaaa cccaagtctt tgggccgagc gacttgctcg acacggtcat ggcgggagga
     5941 caaaagggaa taacccagat gcagcgctat aaaggcctcg gggaaatgaa tccagatcag
     6001 ctctggaaaa caacactcga cagagaagcg cggtcgctgc tgcaggttaa aatcaaggag
     6061 gccgacgacg ctgaggatat ttttgtcaaa ttgatgggag atgtcgtcga gccgcgccgc
     6121 gaatttatac aggaaaacgc tttgagcgtc gctaatttgg atgtgtaagg tttgtttgtc
     6181 aacattgatc cattgacttg cgagtgaaag tctaaccggg gctcttgata ggattcgaag
     6241 ggtgtgagtt aatttaacca gttgccattc agtttatcga ttccactcat tttttgcttt
     6301 tagggggcac gaagtctcac agaataaccc agtcttaatt gtgcatgggt ggagcgacga
     6361 ctacaaatcg ttcgcgccgc tgaaacgcct gttggtaaac gctggttata ggaccgaaga
     6421 tgtgttcttg ggaaactacg cgtcgatgcg ggatgacgtg acgttcgacg atgtgtccta
     6481 cggactgcaa atgcgcctct acgatcttgt gaaaggtaaa aaaatcaaag ggaatgtaga
     6541 taattcggga agattttctt taaatccgtt ttctctggac gtgattgtcc actcgactgg
     6601 aggtcctgta gttcgcaact ggttgagtct ttacctgaga gatgtatgcg gcggagtttt
     6661 cgagaaaagc ccgatccgtc atttcatcat gatggcccca gcgaacttcg gctcccgact
     6721 tgccgcacaa ggaaaatcaa cgctggcgaa attattcaaa ggcggctttg aaacaggccg
     6781 taaaatcctt gaaggccttg agcttggtag cccattttta tgggaccgat cgtgatctat
     6841 tcggcaaagg actatatccg agcacgaatc gaggaacatt tctctacatt ctctctgaaa
     6901 ccgataccta cgggaggctg aagggccttg tggccgtagg tgcaagtgaa aatggttccg
     6961 acggcacgat tcgggcggcg tcggcatcgc ttgacagcag cgtcaaattg gaggtcaact
     7021 tcatcgatcc ggctcatcca caggtcaata tcattactca atctcctgtg ttcaaactcg
     7081 tgaaggaatg caatcactct acgattgttc caagtcccaa cgacacagca gttcacccgg
     7141 ccttcaaaat aattaagcaa tgcctttcta tacaaaatac cactcaatac accacgttac
     7201 gcaatacgtt cgatactgaa aatgaacaac tatacgaaga aaacaaagta tatcgcaagg
     7261 ataatgaatc tgttgatcca attaatcggt tccagcaatt catagtgcga gtcctagaca
     7321 atatgggcaa tgcagtaagc gattaccggc tggacttcca catagtcgat aactcgatcc
     7381 aaaggagtac gtggaccgct cagtcgccac ctcccgcatt agataaatac agcgactaca
     7441 acgtgcgtat catagaaaag gtaatttccg atgtcgagca acatagcgtt gagccgagtt
     7501 atcgaacctt tttcataaac atagatgaac tgaataaact tttgggggat ctagggtctg
     7561 cgcacccaaa tgcatacgtc tcgctgaacc tggatgcgct cccgtccgcg cctggagttt
     7621 cctataacac cgacctcatt aaatacgtcg gactcaatag ctcattcaac ggaaaatcct
     7681 tcgtcaagaa aaacacatca acgctcgttg aaataagatt gcagatacaa gcgtttacga
     7741 tttcccaacg ccaattgagt caaacttccg caaactgagt caattttcgt tgcgattttg
     7801 gcgacggaac gggcaacgtg agtctatgtt cgattttggc ggacatgttt cgcggcccat
     7861 tggggcgaac atgaccgcta tcgactgaat atggagcttc attgtcccga gccgcgcccg
     7921 cctttgaagc gagcgcaatt gtccgcaaat ggcggattta gttgaaaaag tcgctacagt
     7981 gaggctttcg cgcgcgagtt ccccgagg
//
LOCUS       DATMPU010000172         3466 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_3860051,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000172 DATMPU010000000
VERSION     DATMPU010000172.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 3466)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3466)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3466
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3860051"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(12..932)
                     /gene="hemC"
                     /locus_tag="VNY06_03975"
     CDS             complement(12..932)
                     /gene="hemC"
                     /locus_tag="VNY06_03975"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012383024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HWX14011.1"
                     /translation="MRVRFRIGTRGSPLALAQARELCRRLESAHGFEANCITIVAIKT
                     TGDTILNLPLREAGGKGLFTKELDIALLAGNIEIAVHSAKDLPTILPPEIVIVGYLPR
                     EDVRDAWISPNAPHPKKLSAGSVVGTASLRRGAMVKRLRPDLEIALLRGNVQTRLSKL
                     AKGEIAATLLALAGLKRLGLESEATAILDEREFVPAVGQGAIAMTARSGDSAARAFLA
                     PVLDQPTAIALAAERAFLRVLDGSCKTPIGAHASIDEGSLALRAIVLKRDGSKFFETD
                     VLGPVSDAVRLGEMAGQTVAAQIPAGFFDS"
     gene            1067..2125
                     /gene="tsaD"
                     /locus_tag="VNY06_03980"
     CDS             1067..2125
                     /gene="tsaD"
                     /locus_tag="VNY06_03980"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012589059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HWX14012.1"
                     /translation="MRVLGIETTCDETAAAVVQLPPGGCGEILSNEVMSQIAEHVAFG
                     GVVPEIAARAHIETIDRLVERALDRAQLELGDLDGIAAAAGPGLIGGIMVGLTTAKAL
                     ALAIRKPFIAINHLEAHALTARLTDEVAFPYLLLLVSGGHTQLVAVRGVGHYLRLGST
                     VDDAAGEAFDKVGKMLGLPYPGGPHVEKLAAGGNAARFDLPRPMLGRAKPDFSFSGLK
                     TAVRLEVRRIGPLTPADVADVCASFQAAAVDSITDRCRAGLRLFRESVGRPNAMVVAG
                     GVASNGTLRRTLVRFCAEAGLRLVTPPANLCTDNGAMIAWAGIERLSLGLVDDLTFAA
                     RPRWPLDANAEAVHHGKA"
     gene            complement(2138..2749)
                     /gene="ruvA"
                     /locus_tag="VNY06_03985"
     CDS             complement(2138..2749)
                     /gene="ruvA"
                     /locus_tag="VNY06_03985"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018318692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HWX14013.1"
                     /translation="MIGKLTGIFDSQGEDFAILDVHGVGYVVHCSGRTLQKLPKLGEA
                     VTLAIETQMREDSLKLFGFISETEREWFKLLQSVQGVGSKVALAMQTVLDVAELASAI
                     ACQDKAAIARAPGVGPKLAARIISELKDKGATSKMAVGMAAPRVGIDGSQFAAAQDAL
                     SALVNLGYGRPQAAAAVAASVAALGESAPAPDLILFGLKELAK"
     gene            complement(2998..>3466)
                     /locus_tag="VNY06_03990"
     CDS             complement(2998..>3466)
                     /locus_tag="VNY06_03990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX14014.1"
                     /translation="RNMRFASTVLASSLLLSTMTALLPFAQGQDNPSSAAVSRADAPV
                     GSLAPNQAHFLCTAAINSTGTIAGGNFVDPARTFKLGTGRYQVGFLAPCPNVQIANGW
                     FRVIQPDTLNNSVMPDRSCYVADRFGVASALFIECFDHNGSDANTSFTVSVSR"
BASE COUNT          699 a         1025 c         1057 g          685 t
ORIGIN      
        1 ttcaacacgc cctaagaatc gaaaaaaccg gcgggaattt gcgccgcgac ggtttggccc
       61 gccatttctc cgagcctcac cgcatcgcta acaggcccca aaacatcggt ctcgaaaaac
      121 tttgagccat cccgtttgag cacgatcgcc cgtagtgcga gagaaccctc atcaatgctg
      181 gcgtgtgcgc cgatcggcgt cttgcaggaa ccatcaagaa cgcgcaaaaa agcgcgctcc
      241 gccgcaaggg caattgccgt tggctgatcg agaacgggag ccaaaaaagc ccgcgccgcg
      301 ctatcgccgc tccgagcggt catggcaatc gcgccctggc ctacggctgg gacaaattcc
      361 cgctcgtcaa ggattgcggt tgcctcggat tcgagaccca ggcgttttag tcctgcgagc
      421 gcgagcaaag tggcggcgat ctcacctttg gcgagttttg aaagacgcgt ttgaacattg
      481 ccgcgcaaaa gcgcgatttc aagatctggc cgcagccgct tgaccatcgc gcctcgccgc
      541 agcgaagctg tgccgacgac gctcccagcc gaaagctttt tgggatgcgg cgcatttgga
      601 gaaatccaag cgtcgcgcac atcctcgcgc ggaagatagc cgacaatcac tatttccggg
      661 ggaagtatcg ttggcagatc cttggcagaa tgcacggcaa tctcaatatt gccagccagc
      721 aaggcaatgt cgagctcctt ggtaaagagc cctttgccgc cggcctcacg caaaggcaag
      781 ttcaaaattg tgtcgcccgt cgtcttgatg gccacgattg tgatgcaatt tgcttcaaaa
      841 ccatgcgccg actcgagccg ccggcagagt tcgcgtgctt gagcgagcgc caacggactg
      901 ccgcgcgttc caattcgaaa tcgaaccctc aagtttgatt gtgaatcgaa aggagctggc
      961 acattccctc ttgggtttat tggttctata gattgaaaac taacgcggcg ggggccggac
     1021 gacaaaatat ttctgccgcg gtttaggaaa aggcggtttg ggaaacatgc gcgtactcgg
     1081 cattgaaaca acctgcgacg aaaccgctgc cgccgtcgtg caattgccac ctggcggctg
     1141 tggcgaaatt ttgtcgaacg aggtcatgag ccaaattgcc gaacatgtgg cttttggcgg
     1201 cgtggtgccg gagatcgcgg cgcgcgcgca tatcgaaacc atcgaccggc tggtggaacg
     1261 cgccctcgac cgcgcgcaat tggagctggg ggatctcgat ggcattgccg cggccgcagg
     1321 gccgggtctt atcggcggga tcatggtcgg attaacgacg gcgaaggcct tagcgctggc
     1381 aatccggaaa ccctttatcg ctataaacca tctcgaggcg cacgccctca cggcaaggct
     1441 cactgacgaa gttgccttcc cgtatttgct gcttttggtc tcgggcggtc acacccagct
     1501 cgtcgcagtg cggggcgtcg gtcactatct ccggctcggc tcgaccgtgg acgatgctgc
     1561 gggcgaagct tttgacaagg tcggcaagat gcttggcctt ccctatcctg gggggccgca
     1621 tgtcgagaaa ctggcagcgg gtggcaatgc ggcgcgcttc gatcttccgc ggccgatgct
     1681 cggccgggcg aagccggact tttccttctc gggtctcaaa accgccgtcc ggctcgaagt
     1741 gcggcgcatt ggccccttga cgccggcgga tgtggccgat gtctgcgctt cgttccaagc
     1801 tgcggcggtc gattcgatca ctgaccgatg ccgcgcgggc ttgcggctgt ttcgggaaag
     1861 tgtgggccgg cccaacgcca tggtcgtggc cggcggtgta gcctcaaacg gcactctgcg
     1921 ccgcacattg gtacgatttt gcgccgaggc ggggctgcgg ttggtcacgc cgccggccaa
     1981 tctttgcacc gacaacgggg caatgattgc ctgggcgggt atcgagcggc tctcgctcgg
     2041 ccttgtcgac gatctgacct tcgcggcaag accccgctgg ccccttgatg caaatgcgga
     2101 ggccgtgcat catggcaagg cctgatgctc aagggcatta tttggcaagc tccttgaggc
     2161 cgaagaggat gagatcaggg gcaggcgcgc tctcgccaag cgccgcgacc gaagctgcca
     2221 cggcggcggc cgcctgcggc cgcccatagc cgagattgac cagggctgag agcgcatcct
     2281 gcgcagcggc aaattgcgag ccatcgatcc caacgcgagg tgcggccatc ccaacggcca
     2341 ttttggacgt agcgcccttg tccttcaact cggagatgat ccgcgccgcg agttttggcc
     2401 cgaccccggg cgcgcgagcg atcgcggcct tatcctggca tgcaatcgcc gacgcaagct
     2461 cggcaacatc gagcacagtt tgcatagcga gagcgacttt tgagccaact ccttgcacgg
     2521 attgcaacag cttgaaccat tcgcgttcgg tctctgagat aaagccgaac aattttagcg
     2581 aatcctcccg catttgcgtt tcgatcgcaa gcgtaaccgc ttcgccaagc tttgggagct
     2641 tttgcagtgt gcgcccggag caatgcacga cgtaaccgac cccatgcaca tcaaggatcg
     2701 cgaaatcctc gccttgggag tcaaaaatgc ctgtgagttt gccgatcact tcagaaagat
     2761 cgccggaatt ccgagagctt gtcgctatag cgcatcgctc agcgtatcgg aaacccaggt
     2821 cttggatttt ggaggcaatt gttgccggaa tcagcccgta cgccgccgct gtccttgcga
     2881 caagcgcgac cacggcggct ttaacccccc ggaccggcca agaggcggcg cctcttggcc
     2941 tccacatatc aaagttgact gttcagccca gctcttcaga aggttgaccg ccgtgcctta
     3001 ccgcgagacg gagacggtga acgaggtgtt cgcatcgcta ccgttgtgat cgaagcactc
     3061 tataaagagc gcggaagcca caccgaagcg atccgccaca tagcagctgc ggtccggcat
     3121 gacactgttg ttcagcgtgt cgggctggat gacccggaac cagccgttcg caatttggac
     3181 attggggcac ggcgccagga agccgacttg atatcggcct gtgccgagct tgaatgtgcg
     3241 ggccgggtcg acgaaattcc cgcccgcgat tgtgccggtc gagttgatag ccgcggtgca
     3301 aaggaagtgg gcctgattcg gcgcgagaga gcccactggc gcgtcggcac gtgataccgc
     3361 tgcggaagag ggattatctt ggccttgggc aaagggtaag agcgcggtca tcgtgctgag
     3421 cagtagcgaa gaggctagaa cagtgcttgc aaatctcatg tttctc
//
LOCUS       DATMPU010000173         2183 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_447085,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000173 DATMPU010000000
VERSION     DATMPU010000173.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 2183)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2183)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2183
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_447085"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..420)
                     /locus_tag="VNY06_03995"
     CDS             complement(<1..420)
                     /locus_tag="VNY06_03995"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HWX14015.1"
                     /translation="MMPTWKTDLRQLAPNVYVYQQQGGTGFPNAGISNAGLFVGEDWS
                     VALDALGFPLQTKAFLAAAKKATGGKPIAQLINTHHHGDHVAGNQFFLPAQILSHPYC
                     RQEVLKAVPATPKSWPKQEGVADGTEVRKLTPPSITFE"
     gene            complement(757..1734)
                     /locus_tag="VNY06_04000"
     CDS             complement(757..1734)
                     /locus_tag="VNY06_04000"
                     /inference="COORDINATES: protein motif:HMM:NF014841.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HWX14016.1"
                     /translation="MILAVSFEANDAHRKIISETLGDIADIRYFTDLKAAERQDILSR
                     TDVLLARNTAKELRPEELPLLRNARLIQFLSAGIDYIPLNGLPPEIPLAGNGGAYAQQ
                     MSEHAAAMALAAAKRLLIEQQSLLRGQFNQFTPNKVLAGKNCGFFGFGGYGVATARLM
                     RCLGMRIHAINRRGAADEPVDWIGRPDRLPELMAASDVLVISAPLTRATQGAIGEREL
                     SLMKDDAILVNLARGEIIDEVALYEHLKAKPQFTACIDAWWIEPVRHGEFRMNRPFME
                     LPNVIASPHNSASVANAFEVALRHAVANCRRGLMGEKPLHLITPEERMS"
     gene            1803..>2183
                     /locus_tag="VNY06_04005"
     CDS             1803..>2183
                     /locus_tag="VNY06_04005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX14017.1"
                     /translation="MSKRMLRPTISGLALLTFTILGLQSAHSQTTPPHAPDGWDRPVA
                     SSTKAQPGPAPRHDISGTWEPANGPSDGIQGTGSKAMPADGKHEPPYTPLGLELMGRS
                     KPGNGNREVLPADIDDPVFFCNPQG"
BASE COUNT          439 a          640 c          637 g          467 t
ORIGIN      
        1 ttcgaaggtg atgctaggcg gcgtgagctt gcgcacttcg gttccgtcgg ccacgccttc
       61 ttgtttcggc catgactttg gagtggcggg cacagccttc aagacttcct ggcggcagta
      121 agggtggctc agaatttgcg ccggcaggaa gaattggttg ccggccacat ggtcaccgtg
      181 gtggtgcgtg ttgatcagct gggcgatggg cttgccgccc gtggccttct tggcagcggc
      241 aaggaacgct ttagtctgca gcggaaagcc aagagcatcc aacgccactg accaatcctc
      301 tccgacaaac agaccggcat tagagatccc ggcgttggga aaacctgtgc ctccctgctg
      361 ctgataaacg tagacattgg gcgccagttg tcgcagatcg gttttccatg tcggcatcat
      421 aggatatttg tcattcaccg ggggaagcgg cgttaccgct ggccagccca ttcggctgga
      481 ggattcatcc acacgttgcc ccgcctggcc caggagcgcc tcgagaccgc cggctccagc
      541 caacacggaa gcggcaccga cccatgcagt gcgtgcaacc atttcccgcc gtgtcaattt
      601 ttctcccatg gcaacctcct cgaattctga cgttctatgt gcgccggatt atggtggcgt
      661 tcagggagcc tggtcaatgg attttgctcg gcggcgtggc ggctgaacgg cgcatgggca
      721 cacccgcagg agggcgccac cattcggctg atggcttcaa ctcatccttt cttcaggcgt
      781 gatcagatgc aaaggctttt cgcccattag accacgccgg caattggcga ccgcgtgtcg
      841 taaggcgact tcgaacgcat ttgcaaccga tgcggaattg tgtggagaag cgattacgtt
      901 cggcaactcc atgaacggcc gattcatgcg gaactcaccg tgacgtacgg gctcgatcca
      961 ccatgcgtca atgcatgcgg tgaactgagg cttggcttta aggtgctcgt aaagcgcaac
     1021 ttcgtcgatg atttctccgc gcgcgagatt aaccagaatg gcgtcatctt tcatgagcga
     1081 cagttccctt tcgccgatcg cgccctgcgt agcccgcgtg agaggggcac tgataaccaa
     1141 cacgtccgac gccgccatca gttcgggaag ccgatcgggc cggccgatcc agtcaaccgg
     1201 ctcgtccgcc gctccgcgac ggttgatggc gtggatgcgc attcctaggc agcgcatcag
     1261 ccgggccgtc gcaacgccgt aaccgccgaa gccaaaaaac ccgcagtttt tccccgcgag
     1321 gactttgttg ggcgtgaatt gattgaattg accgcgcaaa agactctgtt gctcgatgag
     1381 aaggcgctta gcagcggcga gcgccatggc agcagcatgc tcactcattt gctgtgcata
     1441 ggcgccgcca ttgccggcca aaggtatttc cgggggcagt ccgttaaggg gaatatagtc
     1501 gatacctgca ctcaaaaatt ggatcagccg cgcgttgcga agcagaggca attcctctgg
     1561 ccgaagctcc ttggctgtat tccttgccag taaaacgtct gtgcggctca aaatgtcctg
     1621 gcgctcggca gctttcaaat cggtgaaata ccgaatgtca gcaatgtcgc ctagcgtctc
     1681 cgaaatgatt tttcggtgcg catcgttagc ttcgaacgaa actgcgagta tcatcgtctc
     1741 acccaatcgc ggtatagtgg cgcacaatcg gcccacgatt ttagtccttt tagggaggcc
     1801 ctatgtcaaa acgcatgctg aggccaacga tttcagggct tgcgctcctt actttcacga
     1861 ttttgggcct gcagtccgcg cattcgcaga cgacgccgcc tcatgctccc gatggttggg
     1921 accggccggt agcgagttcc accaaagcgc agcctgggcc ggctccgcgt cacgacattt
     1981 ccgggacttg ggagccagct aacggtccca gcgatggcat tcaggggacc ggctcgaagg
     2041 cgatgcccgc cgatggcaag catgagcccc cgtatacgcc gctcggcctc gaactgatgg
     2101 gccgctcgaa accgggaaat ggcaatagag aagtgctgcc tgcggacatc gacgatccag
     2161 ttttcttctg taaccctcaa ggc
//
LOCUS       DATMPU010000174         2069 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_3242443,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000174 DATMPU010000000
VERSION     DATMPU010000174.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 2069)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2069)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2069
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3242443"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..170)
                     /locus_tag="VNY06_04010"
                     /pseudo
     CDS             complement(1..170)
                     /locus_tag="VNY06_04010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611831.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380 family transposase"
     gene            complement(178..435)
                     /locus_tag="VNY06_04015"
     CDS             complement(178..435)
                     /locus_tag="VNY06_04015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX14018.1"
                     /translation="MPYDDAVLDLIHEARALGRPVYLASASHRSYVSAIVGHLGLFTG
                     WFGSDATTNISGPNKARLLVETFGDRGFDYIGNDRAGHTPP"
     gene            complement(664..1338)
                     /locus_tag="VNY06_04020"
     CDS             complement(664..1338)
                     /locus_tag="VNY06_04020"
                     /inference="COORDINATES: protein motif:HMM:NF014667.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HWX14019.1"
                     /translation="MFKPYCKSLIAAAGIFVVVLPLRAEESTGPPPPGSYTLDHIQRV
                     PAGIVLEGTRFPRALSRYTTGAITLLAFFYSYCADPNGCPLAWDAFEAVREGIFARPE
                     LHGRVRLVFISLDPAHDTPGMLMGFARRYEASASIAPWHFLTTYSNLFLKPVLRDMGE
                     EISIDRGTSSGDGVVINHLLKVFLIDNQGFVREIYSNQSLDPAMILGDIKTLLLEESK
                     ANGDKE"
     gene            complement(1364..>2069)
                     /locus_tag="VNY06_04025"
     CDS             complement(1364..>2069)
                     /locus_tag="VNY06_04025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009759970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008430 - selenium binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="selenium-binding protein SBP56-related protein"
                     /protein_id="HWX14020.1"
                     /translation="KNVFLTSSFTGWNNYMMDLGKLIKDPEAMKHFGNTMVLWNLKSM
                     QPEKIFSVPGAPLEIRWSLTEGDDWAITASALTSKLWLVNHDANDQWQANEVGTIGDP
                     AKIPLPVDISIAADAKTLWVNTFMDGTTHLFDISDPTKPVQIYEKVTGKQVNMISQSW
                     DGKRVYITSSLLKNWDKKGDDNEQVLRAYDWDGKDLKLKFEVDFFAEKLGRPHHMKFS
                     GPLSKASLDMPRAASR"
BASE COUNT          438 a          575 c          602 g          454 t
ORIGIN      
        1 caatcctctc ttgcagaatg cggttcgccg gcaatcggat cgcatatttg atccgctcgg
       61 cccgcgtcag cccgaaaata aatgcgcgag aacttgccgc gataacgcgc cacgacctgc
      121 ttcagcacat tttcccggcc atcggcgctg tggacattgc cggggcgcag gcgcatatca
      181 gggaggtgta tggccagctc gatcgttgcc gatataatcg aagccgcggt ccccaaatgt
      241 ctcgacaagg aggcgggcct tgtttggtcc tgagatattc gtggtcgcgt cggagccgaa
      301 ccagccggtg aagagcccaa gatggccgac gatggccgac acatagctgc gatgactggc
      361 ggaggcgaga taaaccggcc ggcccaaggc ccgagcctca tggatgaggt ccagaaccgc
      421 atcgtcgtat ggcaaataag cgggatcgaa ttgaaccgcc cgcgccagca gatccttgaa
      481 atgtgctaga gatgaggtgg agggctttca tttcgtgttg aagtaaatgc cgtctcccag
      541 tgagccgttg tactgtcagt ctcgaaacag ccgggcgtca tccatgctca tatcgcactc
      601 ctttgaagac gccctgggca gtgacgcacg ttcaagaggt gcggctcgtc cctttgccaa
      661 tgctcattcc ttatctccgt tggccttcga ctcttcgagc agcagcgtct tgatgtcgcc
      721 aaggatcatg gcgggatcga gcgattggtt cgagtagatc tcgcggacaa agccctgatt
      781 gtcgatcaaa aatactttta ggagatgatt tatgaccacg ccgtcaccgc tcgacgtccc
      841 gcgatcgatg gaaatctcct cgcccatgtc gcgcagtacg ggtttgagaa ataaattgga
      901 ataggtggtg aggaaatgcc agggcgcgat ggacgcgctc gcttcgtagc ggcgtgcaaa
      961 acccatcagc atccccggcg tatcatgcgc tgggtcgaga ctgatgaaga cgagccggac
     1021 tcgtccgtgc aattcggggc gcgcgaagat gccttcccgc actgcctcga acgcgtccca
     1081 ggcaagcgga cagccgtttg gatcggcgca ataggaataa aaaaacgcaa ggagcgtgat
     1141 cgcccccgtc gtgtagcggg aaagcgcgcg gggaaaccgc gtgccttcga gcacgattcc
     1201 cgcgggcacg cgctgaatat ggtcgagcgt atagcttcca ggcggcggcg gtcccgtgga
     1261 ctcttccgcg cggagcggta ggacgaccac aaaaatccct gccgccgcga tcaaactttt
     1321 gcaatacggc ttgaacaaaa aaatgctccc gcctttgcct tcttcaccgc gatgccgcgc
     1381 gcggcatgtc gagcgaggct ttactgagcg gacccgaaaa tttcatgtga tgcgggcggc
     1441 cgagcttttc ggcaaaaaaa tcgacctcga atttgagttt cagatccttg ccgtcccagt
     1501 cgtaagcgcg caggacctgt tcgttatcgt cgcctttctt gtcccaattt ttcaacagcg
     1561 aggaggtgat atagacgcgc tttccatccc agctttgcga aatcatgttg acctgcttgc
     1621 cggtgacttt ctcgtagatc tgcaccggct ttgttggatc ggaaatgtcg aacaaatggg
     1681 tggtgccatc catgaacgtg ttgacccaca aagtcttggc gtcggccgca atcgaaatat
     1741 cgacgggcag cggaattttt gcgggatcgc cgatcgtgcc gacctcgttg gcctgccatt
     1801 gatcgttggc gtcgtggttg acgagccaaa gttttgatgt cagcgccgag gcggtgatcg
     1861 cccaatcgtc gccttctgtc agcgaccagc ggatttcgag cggtgcgccg ggaacgctaa
     1921 atatcttttc cggctgcatg gatttgaggt tccacagcac catggtgttg ccgaagtgct
     1981 tcatcgcttc gggatccttg atgagcttgc cgaggtccat catgtaattg ttccagccgg
     2041 tgaagctcga cgtcaaaaac acgttcttc
//
LOCUS       DATMPU010000175         2837 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_769502,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000175 DATMPU010000000
VERSION     DATMPU010000175.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 2837)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2837)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2837
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_769502"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..556
                     /gene="rmuC"
                     /locus_tag="VNY06_04030"
     CDS             <1..556
                     /gene="rmuC"
                     /locus_tag="VNY06_04030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012386159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HWX14021.1"
                     /translation="DAKFPLEAFTALRQASSEESRKQAAARVRADVSKHIRDIAERYF
                     IPEETQDIAIMFVPSESLYADLHEFFDDIIQRAHKSRIIIVSPSLLMMAVQVMQAIVR
                     DARVREEAHVIQAEVRYLVEEVTRLRVRAAKLEAHFKIAQEDIDQIMTSADKVARRGG
                     RMERMDFTTPAAEIDVLPFGKAAE"
     gene            complement(599..964)
                     /locus_tag="VNY06_04035"
     CDS             complement(599..964)
                     /locus_tag="VNY06_04035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012589327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HWX14022.1"
                     /translation="MANPFVHVELATSDIGKAKSFYSALFDWKLEDMEMGPGFTYTMI
                     RVGEGTGGGMMTTPAPGVPTAWLPYVLVDDVTAATAKAKSLGATICKDVTDVPGAGSF
                     SVIADPTGAAIGLWQEKAA"
     gene            complement(1141..1746)
                     /gene="recR"
                     /locus_tag="VNY06_04040"
     CDS             complement(1141..1746)
                     /gene="recR"
                     /locus_tag="VNY06_04040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010913286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HWX14023.1"
                     /translation="MAKRVAGPEIERLIQLLARLPGLGPRSARRAALHLIRKRDALLA
                     PLADALQAARETIVTCQICGNVDSRDPCTLCRDERRDRGTLIVVETVADLWALERARA
                     LSARYHVLGGTLSPLDGIGPKDLNLTSLIDRVAADHVKEVILAVNATVDGQATAHYIT
                     SRLADSTVKITRLALGVPVGGELDYLDEGTLAAAIRSRTLF"
     gene            complement(1768..2091)
                     /locus_tag="VNY06_04045"
     CDS             complement(1768..2091)
                     /locus_tag="VNY06_04045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012589324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HWX14024.1"
                     /translation="MRDIFDLMKQAQAMQAKMQEMQAEMEQIEVEGQAGGGLVRITLT
                     AKGRMKALAIDDQLLKADEKVILEDLIVVAHEDARKKAERLLEEKMKGVTAGLSLPPG
                     MKLPF"
     gene            complement(2146..>2837)
                     /locus_tag="VNY06_04050"
     CDS             complement(2146..>2837)
                     /locus_tag="VNY06_04050"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF023780.4"
                     /note="catalyzes the DNA-template-directed extension of
                     the 3'-end of a DNA strand; the tau chain serves as a
                     scaffold to help in the dimerizaton of the alpha,epsilon
                     and theta core complex; the gamma chain seems to interact
                     with the delta and delta' subunits to transfer the beta
                     subunit on the DNA; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HWX14025.1"
                     /translation="IKDSPRPLIAADMVLVRIGFAADLPTPGEALRKLMSATTGQTPD
                     GPQNREAEIVSRAPLSAENASLALASAPPPNAALPSIALSRFEDIVALAGRHRDIQLK
                     LALERDVRLVRFEQGRIEFSVASGASPRLAHTLMSRLQEWTGSRWLVAVSSTPGAPSL
                     KEQEEAKAREILTGVHFDPLVQSVLAAFPGAEIVGVRTNKIALENPLAAGTDNKMNDE
                     IGFHDDLDDDP"
BASE COUNT          587 a          840 c          815 g          595 t
ORIGIN      
        1 cgacgcgaaa tttccgctcg aggctttcac agctttgcgg caagcgtcaa gcgaagagtc
       61 acgcaaacag gcggcagcaa gggtgagagc cgatgtgtcg aagcatattc gcgacatagc
      121 agagcgctat tttatcccgg aggaaaccca ggatatcgcg atcatgttcg tgccttctga
      181 atcgctttac gccgatcttc acgaattctt tgatgatatt attcaaagag cacataagag
      241 ccgcatcatc atcgtctcac cgtcgctgct catgatggcg gttcaggtta tgcaggcgat
      301 cgtccgcgac gcgagggtgc gggaggaagc tcatgtcatt caggcagaag tgcgctacct
      361 tgtcgaagag gtcacgcgcc tgcgggtgcg cgcggcgaag ctcgaagctc atttcaaaat
      421 cgcgcaagag gacatcgacc agatcatgac gtctgctgat aaagtcgcaa gacgcggcgg
      481 acggatggag cgaatggatt ttaccacccc cgctgccgag atcgacgtcc tgccattcgg
      541 caaggccgcg gaatgagtta cgcgttgaac gggtagttgg cggcccgaag cgaccgtgtt
      601 atgccgcctt ttcctgccac aggccgatcg ccgcaccggt tggatcggcg atgacggaga
      661 acgagcctgc gcctggcaca tcggtgacgt ccttgcagat ggtcgcgcca agcgattttg
      721 ccttggcggt cgccgccgtc acatcatcga ccagcacata gggcaaccag gcggtgggga
      781 cgccgggagc gggagtcgtc atcatgccgc ctcccgtgcc ctcgccgact ctgatcattg
      841 tataggtgaa accgggcccc atctccatat cctcgagctt ccagtcgaac aatgctgaat
      901 agaacgactt tgccttgcca atgtcagagg tggcgagttc gacatgcaca aatgggttag
      961 ccatgaccgc tcctttaaga ctattttagt ccagtttccg tgcgcacggt tgaacaacgc
     1021 gttaccaagc cgctggcgtt acccccgctg gccaaaacca cgtgctgcta tatcttgggc
     1081 agctcttgcc catgtaaagt atcgctatgg cgattgtatg atacggctac ggacgcgcga
     1141 ctaaaataat gtccggcttc ggattgcggc ggcgagcgtg ccttcatcga gatagtcgag
     1201 ttcgccgccc acaggcacac cgagcgcgag acgcgtgatc ttgaccgttg aatctgccaa
     1261 tcggctggtg atataatggg ctgtcgcctg tccatcgacc gtggcgttga cggcaagaat
     1321 gacctctttc acatgatcgg ccgcaacgcg atcaatgagg cttgtgagat tgaggtcctt
     1381 tggacctatg ccgtcaagtg gcgacagtgt cccgcccagc acatgatacc gtgcgctgag
     1441 cgctctggcg cgttcgaggg cccaaaggtc ggcgacggtc tctacgacga tcagcgtgcc
     1501 acgatcgcgc cgttcgtccc tgcacaaggt gcaaggatca cgactatcca cattgccgca
     1561 gatctggcag gtcacaatgg tttcacgcgc cgcctgcagc gcgtcggcga gcggcgccag
     1621 aagggcatca cgcttgcgaa tgagatgcaa cgccgcgcgc cgcgccgagc gcggtccaag
     1681 ccccggcaaa cgggcgagca attggatgag ccgctcgatc tcgggacccg cgacccgctt
     1741 cgccatcgca gccgtgccct caggctatca gaacggcaat ttcatgcccg ggggcaatga
     1801 gaggccagcg gttacgccct tcatcttctc ttccaaaagc cgctcagcct ttttacgcgc
     1861 gtcttcgtgc gcgacaacga taagatcttc gaggatcacc ttttcgtcgg ctttcagaag
     1921 ctgatcgtcg atcgcgagcg ctttcatccg gcctttggcg gtaagggtaa tccgaaccag
     1981 gccgcctccg gcttgccctt cgacctcgat ctgctccatt tcggcctgca tttcctgcat
     2041 tttcgcctgc atggcctgag cttgcttcat caaatcgaaa atgtcgcgca ttctaattac
     2101 cctttgtttg ctgcgacgaa caggaaaggc gcgaacgatg cccgctcaag ggtcatcatc
     2161 cagatcgtca tgaaagccga tttcgtcatt cattttgttg tccgttccgg ccgcaagcgg
     2221 attctccagt gcaattttat tcgtgcggac gccaacaatc tcggcgcccg gaaaagcggc
     2281 gagcacgctt tggacgagcg gatcgaaatg cacgcccgtc aaaatttcgc gtgcctttgc
     2341 ttcttcttgc tccttgaggc tcggcgcccc aggcgtgctt gaaaccgcca ccaaccatcg
     2401 gctgccggtc cactcctgca ggcggctcat gagcgtatgc gcgagccttg gtgaagctcc
     2461 agacgcgact gagaattcga tgcggccttg ttcaaaacgg acgaggcgca catcgcgttc
     2521 aagcgcgagc ttcaactgaa tatcgcgatg ccgtccagcg agcgcaacaa tgtcttcgaa
     2581 ccgcgagagt gcaatcgacg gcagggctgc attcggcggc ggcgcggacg caagcgcaag
     2641 gctcgcgttt tccgcggaca aaggggcgcg ggatacaatt tccgcctccc ggttttgtgg
     2701 tccatccggc gtttgcccag ttgtagccga cataagttta cgcaaggcct cgccgggcgt
     2761 cggcaaatcg gcggcaaaac cgatccggac caaaaccata tcggcggcaa tgagcgggcg
     2821 aggcgaatct tttattt
//
LOCUS       DATMPU010000176         4464 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_2940252,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000176 DATMPU010000000
VERSION     DATMPU010000176.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 4464)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4464)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4464
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2940252"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..210)
                     /locus_tag="VNY06_04055"
     CDS             complement(<1..210)
                     /locus_tag="VNY06_04055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX14026.1"
                     /translation="MSDESEEPQGAAEDTKPPKIKAEDNPWCLLATLFGVPGKTSDEQ
                     LLEMNRIAWNRYFAADVDEETRARLI"
     gene            352..864
                     /locus_tag="VNY06_04060"
     CDS             352..864
                     /locus_tag="VNY06_04060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX14027.1"
                     /translation="MYLTLGQAAREAGVAKSTISKALASGKLSYREKNSDGYKIDPAE
                     LFRVYPKTAKTDADQLLSNDWRQDEVGAETLHYSAKFEIQLAGLKSLIGEKDRRISDL
                     EADRAQLREDRHRLTENWQEERVRLLKLIEDQTCTVKLLTDERSKLAAEMQRTFWQRL
                     FHRKAAVAAA"
     gene            1026..2261
                     /locus_tag="VNY06_04065"
     CDS             1026..2261
                     /locus_tag="VNY06_04065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012589786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HWX14028.1"
                     /translation="MPKPYTAHAAQSIGEIDAAEWDACANPEADTARLGAPPPGPQSQ
                     CIKEQTAYEVERYNPFITHAFLKSLETSRSVGPKTGWISAHVLIRDAIGRLAAAAPAY
                     FKTHSMGEYVFDHGWADAYHRAGMRYYPKLQVAVPFTPVTGRRLLLAPGYGEEARETL
                     IAGLRALRAKARASSLHITFPTRSEWELLGTCGFLQRSGQQFHFFNKGYTDFDAYLSD
                     LASRKRKMIRRERKQALANGIEIELLTGSSILERHWDAFFCFYMDTGARKWGTTYLTR
                     QFFSSVGASMNQNILLVMAKLDGRYIAGALNFLGKDAVYGRNWGATEDHPFLHFEVCY
                     YQAIEFAISHGFARVEAGAQGEHKLARGYRAVPTFSAHDISDKRFSPAIEDYLRRESL
                     YVDEALREYEDAAPFKKGS"
     gene            2381..2551
                     /locus_tag="VNY06_04070"
     CDS             2381..2551
                     /locus_tag="VNY06_04070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX14029.1"
                     /translation="MSESEAFDPYSLLNVLKLIAQDVWIVDGPEIRFSYCGFPLPFPT
                     HMTVIRLPDGSL"
     gene            3163..3597
                     /locus_tag="VNY06_04075"
     CDS             3163..3597
                     /locus_tag="VNY06_04075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013531772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HWX14030.1"
                     /translation="MPSSAYDDANIFAKILRGELPCHKVYEDEVALAFMDILPRADGH
                     VLVLPKAPARNLLDADAESLSKLIIRVQKIARAVKSALTADGITILQYNESAGGQIIF
                     HLHFHILPRWHGVELRPHTGIQEKPEVLEGICKKIAAAIEIA"
     gene            complement(4064..4408)
                     /locus_tag="VNY06_04080"
     CDS             complement(4064..4408)
                     /locus_tag="VNY06_04080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019905888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0050898 - nitrile metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrile hydratase accessory protein"
                     /protein_id="HWX14031.1"
                     /translation="MFATFEEYATTSMFGSPDSPPRLDGKLLFANRWERDVFGLALSL
                     SKAGYFEWEDFRQCLIAAIARWEAAPCEGQPRWDYYERFFEALLDVIESSGVLSDDEL
                     AQVLVAREVASP"
BASE COUNT          958 a         1304 c         1190 g         1012 t
ORIGIN      
        1 gatgagccgt gcgcgcgttt cttcatccac atccgcagcg aagtaacggt tccacgcgat
       61 ccgattcatt tctaataact gttcgtcgct tgtttttcca ggcacgccga agagtgtcgc
      121 caacaaacac cagggattgt cctcggcctt tattttcggc ggctttgtgt cttccgcggc
      181 gccctgaggc tcttccgact cgtcactcat cgtcggtcag ctcccctccc cctgctccaa
      241 aaccccgcgc aatcttatta cggcctcggc atttcccgat tcgcgcttaa agttttctta
      301 aatttttggc gaaatagttt ccaaacaccc acgaaaacga ttggaaaccg catgtatctc
      361 accctcggac aggctgccag agaggcaggc gtcgccaaat cgaccatctc caaagccctg
      421 gcctccggca aactttccta ccgcgagaaa aattcggacg gctataagat cgatcccgca
      481 gaacttttcc gtgtgtatcc gaaaaccgcg aaaaccgacg ccgatcaact cttgtcgaac
      541 gattggcgac aggatgaggt cggcgcggaa actctgcact attcggcaaa attcgaaatt
      601 cagctcgccg gactaaagag cctcatcgga gaaaaggacc gccgcatcag cgatctcgaa
      661 gccgaccgcg cgcagctgcg tgaagaccgc caccggctga ccgagaactg gcaggaagag
      721 cgcgtccgcc tcttgaaatt gattgaggat cagacctgca ccgtcaagct tctgaccgac
      781 gaacggtcga aactcgccgc ggagatgcag cgtacatttt ggcagcggct tttccaccgg
      841 aaggccgcgg tcgcggcagc ctagaccaag atggcttttt tcaaatcgcc atcttgttgt
      901 aatcttattt tgtcgcgtgt tcgggcacag aacggttgcc gcttttgccg gacgcgctct
      961 gagcgcttcg attgcacgcg cgggccaggc cggggtagaa atcatctcta ccctggaagc
     1021 ccgcgatgcc aaagccttat acggcccatg ccgcgcaatc gataggcgag atcgacgctg
     1081 ccgaatggga tgcctgtgcc aatcctgagg cggatacggc gcgccttggc gcccctccgc
     1141 cgggccctca atcacaatgt atcaaagagc agaccgcata tgaggtcgag cgatataatc
     1201 cgtttatcac ccatgctttt ttgaagtcgc ttgagacatc aaggtccgta gggccgaaaa
     1261 cgggctggat cagcgcgcat gttcttataa gggacgcaat cggccggctt gcggccgccg
     1321 cacctgctta ttttaaaacc cattccatgg gcgaatatgt gttcgatcat ggctgggcgg
     1381 acgcctatca ccgcgcaggg atgcgctatt atcccaaact gcaagtcgcc gtgccgttca
     1441 cgccagtcac cggccgccga ctcttgcttg cgccgggata tggtgaggag gcacgcgaga
     1501 ctctgattgc cggtcttcgt gctctgcgcg ccaaggcccg agcatcctcg cttcacatca
     1561 cttttccaac acgcagcgaa tgggaattgc tcggcacatg cggctttctg caacgcagcg
     1621 gacagcaatt tcactttttc aacaaaggct ataccgattt cgatgcctac ttgtccgatc
     1681 ttgcctcgcg caagcgaaag atgatccggc gcgaacgaaa gcaagctctt gccaatggca
     1741 ttgagatcga gcttttgacg ggctccagca tactggaacg ccattgggac gcgttttttt
     1801 gcttttacat ggacactggc gcgcgcaaat ggggaacgac ttacctcact cgccaatttt
     1861 tctcctcggt gggcgcctcg atgaaccaaa atattctttt ggtcatggcc aagctcgacg
     1921 gacgctatat tgcaggtgcc cttaattttc ttggcaagga cgcggtctac ggccgcaatt
     1981 ggggagcaac cgaggaccat cccttcttgc atttcgaggt ttgctattat caggcgatcg
     2041 aatttgcgat ttcgcacggc tttgcccgcg tcgaggccgg tgcccaaggc gaacataagc
     2101 ttgcgcgcgg ctaccgcgcg gtcccgacgt tctcggccca tgacatcagc gacaaacgct
     2161 tttcccccgc gatcgaggat tatctccgga gagaaagcct ttacgtcgat gaggcgctac
     2221 gcgaatatga agatgcagcc ccattcaaga aggggtcgtg attcccttcg aattgaatca
     2281 agctgaaaaa tgccttgatc atggtcatcc accatcggcg gcccgccttt tgtggtcgcg
     2341 cggaatccat gcgctccacg tcaggacggg gctttgcggc atgagcgaaa gcgaggcgtt
     2401 tgacccctat tcccttttga atgtactgaa gctaattgct caagacgtct ggattgtgga
     2461 cggacccgag atacggttca gctactgtgg cttccctctt ccctttccaa cccatatgac
     2521 agtgatccgg ctgccagatg gcagtctctg agtccattca ccaaccgctc caaccgagat
     2581 agtggcaggt caaatccaag aggctgggcc ggtaggtctc ctgatcgcca aacacgcttc
     2641 attattggtg gattgtcgac tggaagaggc gtttttcgaa cgcttaagtt tacgcggcgc
     2701 cagggcttgc gcgctcggcg aaacggtcgc tgccgataga tcatgtcttg gacattgcgc
     2761 caccgtggac gggcgttatt gaccaaacaa ttgcgcgtgg cgacgtgctg accgaggtca
     2821 tatcttccac cacctttctc gtacgcttgt gtttacggac ttgattgaga acttcgagcc
     2881 acagcgaacg cgaagcaggc tgtatcgttt tttctttata ttgcaggcgc cgccgaccat
     2941 ggaaaggctc caatcgacat gcggttgacg gtttggcgtc atagtagggc tttgagggcg
     3001 gcagtggaac gcatgattgc gtggcacccg gaaaggatta tcatcgcgca tggcagatgg
     3061 tatgagggaa cttcgaggcg gagttgcggc gttcttttcg attgggtctt gcgcaactga
     3121 tgatgatcaa tgaattgaac ctgagaattg tggaacggaa ccatgccctc ctctgcctat
     3181 gacgacgcaa atatcttcgc gaagattcta cgcggcgagc taccctgtca caaggtttat
     3241 gaggatgagg tggcactcgc ctttatggat atcctgccac gcgcggatgg ccatgttctt
     3301 gtcttgccca aagcacctgc gcgcaatctc ctcgacgcgg atgcggagag cctctcaaag
     3361 cttattatcc gtgtccaaaa aatcgcgcgt gcggtcaaat cagcccttac cgctgacggg
     3421 ataaccattc tccaatacaa tgaatccgct ggcggtcaga tcattttcca tctgcacttt
     3481 cacatccttc cccgatggca cggggtggag ctgcgtccgc atacgggtat ccaggaaaaa
     3541 cctgaggtcc tcgaaggaat ttgcaagaaa atcgcagcgg cgattgaaat agcgtagccg
     3601 gaagcttgcg tgtcatacca gccggcctca tttttgactc cgggaaggga ttcccgaata
     3661 ggccaaactc cggctcctga acccatagcg ttgaagaagg cgtcgagttg acgcgttggc
     3721 cccaatgaac ttgtttagaa taactctaag aatgtttcac gtaaaacatt tttgtccgat
     3781 taaggctcaa aaccttacaa ggccggagac atccttatcg tcttcaatct tgtaccattg
     3841 cccgctttta tggtgcaatt gaatggcgcg ctgtcacggc cgcagtggcg aggcgcgctg
     3901 tcgaaccgca tgactgatgt aagtttcgct atattcgcgc tgtcgcgcgg cccctcagtg
     3961 cttcgtttca ggctcgccgc ttgggccaat cgtctcggcc tcgtcgacat cggggccagc
     4021 caggtcttca cctggctcgt cgtcatcacc gccatcttca cccttatggc gaagcgactt
     4081 cgcgcgccac cagcacctgg gccagctcgt catccgacag cacgccgctc gactcgatca
     4141 cgtccagcaa tgcctcaaaa aaccgctcgt agtagtccca gcgcggctgc ccctcgcacg
     4201 gcgccgcttc ccatctcgcg atggccgcta tcaggcactg gcgaaagtcc tcccactcga
     4261 agtagccggc cttggagagc gacagcgcga gcccgaacac gtcccgttcc cagcgattcg
     4321 cgaaaagcag cttgccgtcg agccgcggcg gcgagtcggg gctgccgaac atcgacgtcg
     4381 tagcgtattc ctcgaaggtt gcgaacattt agggctccta cgcagctggt gcggcgacaa
     4441 gcgccactcc catcatcgat tccg
//
LOCUS       DATMPU010000177         4093 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_1390107,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000177 DATMPU010000000
VERSION     DATMPU010000177.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 4093)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4093)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4093
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1390107"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..664
                     /locus_tag="VNY06_04085"
     CDS             <1..664
                     /locus_tag="VNY06_04085"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HWX14032.1"
                     /translation="FITAARLIDWKRLAYAVRASRFDAILVFATAFAAVFISVEDSIL
                     IGVAISLLLFVPRVSKLGIRELIVTPERVVREREPDDPRSSSLLIYDLEGELFFGAAP
                     ELDHYLDEIKQETISTNIKYVVLRLRRTRTPDVVAIEHLERFLRDAEKRGVTVLLAGV
                     RPNLAEILRNVRFNEWLPPDRIYPEKDETFSATLNAVRHAYRLLNQGSKLEEKEAVYY
                     LV"
     gene            complement(1188..1646)
                     /locus_tag="VNY06_04090"
     CDS             complement(1188..1646)
                     /locus_tag="VNY06_04090"
                     /inference="COORDINATES: protein motif:HMM:NF028108.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5069 domain-containing protein"
                     /protein_id="HWX14033.1"
                     /translation="MSTQIAPTDLTQRPPRSPRCRLGGYVILPRILDKGRAKLAGKNG
                     EYNYNSPTDQHFVRFLGVDLEALAKELASGKADGEILEWIQAHAKTPRAPWEIEVWSG
                     YMEKRGPDSDAETLSLFAEYVGQHSKSREDIKTWFDALDLDDYVSFGGKA"
     gene            complement(1739..2848)
                     /locus_tag="VNY06_04095"
     CDS             complement(1739..2848)
                     /locus_tag="VNY06_04095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018181817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWX14034.1"
                     /translation="MASLTISNLNKSFGSVAVLQGIDLEVKTGEFVVLVGPSGCGKST
                     LLAMIAGLESISAGEIRIGERLVNDVPPKDRDIAMVFQSYALYPTMTVRANITFGMES
                     RGVPKQAQAKALANVAGLLQIESLLGRKPSQLSGGQRQRVAMGRALVRDPALFLFDEP
                     LSNLDAKLRVEMRTEIKKLYQRLAKTTIYVTHDQVEAMTLASRIAVMHQGRLQQFAEP
                     RTVYDRPANMFVAGFMGSPPMNFLPAQIAASGPAAVVLTESEGQAMRLPLPDKVSASA
                     GGGRRVVLGIRPENLLRYDRRLAEEKPYLATIKAPVEVVEPTGAETMTIVRIGTREII
                     ARFEPDAAPSVGETVTLAVDMAKACLFDPETEKLI"
     gene            complement(2858..3751)
                     /locus_tag="VNY06_04100"
     CDS             complement(2858..3751)
                     /locus_tag="VNY06_04100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018181818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HWX14035.1"
                     /translation="MAETVAYDPLGSAGTWRRYRAKLVIYTLLGLFALYYLLPLVVVV
                     LNSFRDLSDISRNGLINLPHSFSLDYWSLAWDKFCVGGTCAGIEPFFFNSVAMVVPAT
                     TISTAIGSINGYVLSKWRFAGSEVLFGLMTLGVFMPGQMVLLPWARVLGWLGLTNSTT
                     GLVLIHCVQGLSFTTLFFRNYYVNIPDDLIKAARIDGAGFWRIFRKIVLPLSPPIVIV
                     TVIWQFTNIWNEFLFAVIFSSGTHQPITAALIALSAGGTSVHQYNVESAAVLIAALPP
                     LLVYFFGGRYFLRGLTQGAIK"
     gene            complement(3744..>4093)
                     /locus_tag="VNY06_04105"
     CDS             complement(3744..>4093)
                     /locus_tag="VNY06_04105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018181819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HWX14036.1"
                     /translation="VDPDLVKAAQIDGANMPRIYRRIILPAIGPIFIAVLVVLLQFAI
                     KTFDLVIALTGGGPGIATTVPAIYVYDLMFQRGQMAQGAAAAIMIFLALSLVLVPYTI
                     WTNWRQRREVVRG"
BASE COUNT          880 a         1181 c         1179 g          853 t
ORIGIN      
        1 ctttatcaca gcggcccggt taattgactg gaaacggctc gcctatgcag tccgcgcctc
       61 ccgcttcgac gcgatactcg tctttgctac ggccttcgcg gcggtgttta tcagcgtcga
      121 ggattcgatc ctgattgggg tggcaatttc cctcctctta tttgttcctc gggtttccaa
      181 gctagggatc cgagaactca ttgtcacgcc cgagcgcgtg gtgcgcgagc gggaaccgga
      241 cgacccacgt tcgagttccc tcctcattta tgatctcgaa ggcgagcttt tcttcggagc
      301 ggcacccgaa ctggatcact acttagacga aatcaagcag gaaaccatca gcactaacat
      361 caaatacgtc gtccttcgcc taaggcgcac tcgtaccccc gatgtggtgg caatcgagca
      421 cctggagcgt ttcctgcgtg acgccgaaaa acgtggggtt accgtactct tagccggtgt
      481 tcgccctaat cttgccgaaa tcctgcgcaa tgtccgcttc aacgagtggc tccctccgga
      541 ccgcatttat ccggaaaagg atgaaacctt ctcggccact ctcaatgcgg tgcgtcatgc
      601 ctatcggctc ttgaatcaag ggagcaagct tgaggaaaaa gaagcggtct attatctagt
      661 gtagaaaaca tcgaaatcgc tggcacattc aatcaggcgt tgaaggccag aaatactacc
      721 aacataaatt ttttacgttt tcgtatttga aatttcgtca ttccaaccat cgaattagtg
      781 cgcacacctg cacaaatcac accgtaccct acgggacggc tcttttgggg tggcgctgtc
      841 ccaggcgctt cgtgccaggc tacgatcaca ccgtccctcc gggacatttc ggacaggctt
      901 ttagctatcg ccctgcaagg tcgactgggg ttcggcgcag gcaccgctct cgctcaggat
      961 aatttccact aggaacccca tcactataag catgagtcta ccggggcaag aatcgcggac
     1021 acgggagttt tggcgcttcg tccgcgcccc gcgcctcgcg gatcaggagg ttgctcctga
     1081 gagcttttgc tctttatcac aaaatgggtg gaaaaatgct ccagcgattt cgctcattgg
     1141 cggaaagcca ccatcaacgg ccaggatggg tccagtaaaa aaacgtgtta ggctttcccg
     1201 ccgaagctga cgtagtcgtc gagatcgagg gcgtcgaacc aagtcttgat atcttcgcgg
     1261 ctcttgctgt gttgcccaac gtattcggca aaaagggaca gagtttcggc atcactgtcc
     1321 ggtccccgtt tttccatgta ccccgaccag acctcgattt cccaaggcgc gcgtggggtc
     1381 ttggcgtgag cctggatcca ctcgaggatt tctccgtcgg cctttccgct agccagctct
     1441 ttcgcaaggg cttcgaggtc gacgccaagg aacctgacga agtgttgatc ggtcggcgag
     1501 ttgtaattgt attcaccatt ttttccggca agctttgccc ggcctttgtc aagaattcgc
     1561 ggcagaatta cgtatccgcc aaggcggcaa cgcgggctgc gcggcgggcg ttgggttaag
     1621 tcagtcggtg cgatctgggt gctcattctt tagcttggtt gaatcactct attgacgttc
     1681 ggcgttcggc ggctccttgg ctccttggct ccttggctcc ttggccctgg cccctggctc
     1741 aaatcaactt ttccgtctcc ggatcgaata ggcacgcctt ggccatatcg actgcgagtg
     1801 tcaccgtctc gccgaccgag ggcgctgcgt ctggctcgaa gcgggcgatg atttcccggg
     1861 tgccgatgcg gacaatcgtc atggtctcgg cgccggtcgg ctcgacgacc tcgactgggg
     1921 ctttaatcgt ggcgagatag ggcttttcct ccgccaggcg acgatcgtaa cggagcagat
     1981 tttccggccg tatgcccagc accacgcggc gacctccgcc ggcggatgct gaaaccttgt
     2041 cgggaagcgg caggcgcatc gcctgtcctt cgctttccgt caataccacc gcagccggcc
     2101 ccgaagcggc aatctgggcg ggcaggaaat tcattggcgg cgaacccatg aagccggcaa
     2161 cgaacatgtt cgccggccgg tcgtagaccg tccgcggctc ggcaaattgc tgcaacctgc
     2221 cctgatgcat gaccgcgatg cgggaggcga gcgtcatcgc ttcgacctgg tcgtgggtga
     2281 catagatcgt tgtcttggcg agccgctgat aaagcttctt gatctcggtg cgcatctcga
     2341 cgcggagctt ggcatcaaga ttcgagagcg gctcgtcgaa caggaacagc gcgggatcgc
     2401 ggaccagcgc ccttcccatc gcgacgcgct gacgctggcc gcccgagagc tgggagggtt
     2461 tgcggccgag cagactttcg atctgcagga gcccggcgac attggcaagg gctttcgctt
     2521 gcgcctgttt gggtacgccg cggctctcca tgccaaaggt aatattagcg cggaccgtca
     2581 tcgtcggata gagcgcgtag gattggaaga ccatggcgat gtcccgatcc ttgggcggaa
     2641 cgtcgttgac caagcgctcg ccgatcctga tctcgccggc gctaatgctt tccaggccgg
     2701 cgatcatggc gagcaaggtc gacttgccac aacccgaagg tccgaccagc accacgaatt
     2761 cccctgtctt cacctccagg tcgatgcctt gcagtacggc aacagagccg aaggatttgt
     2821 tcaggttgct aattgttaag gaagccatgt ctatctgcta tttgattgcg ccctgggtca
     2881 gcccgcgcag aaaataccgg ccgccgaaga aatagaccag gagtggcggc aaagcggcaa
     2941 tcagcacggc ggcgctttcg acattgtatt ggtgaaccga cgttcccccg gcgctaagcg
     3001 cgatcagggc agcggtgatc ggttggtggg ttcctgaaga aaagatgacc gcaaagagga
     3061 attcgttcca gatattggta aattgccaaa tcacggtgac gatcacaatg ggcggcgaaa
     3121 ggggcagcac aatcttccga aaaatcctcc agaaaccggc gccgtcgatc cgcgccgcct
     3181 tgatgagatc gtcgggaata ttgacgtaat agttgcgaaa gaacagcgtc gtgaagctca
     3241 gtccctggac gcaatggatg agcaccagac cggtagtgga attggtcagg ccgagccagc
     3301 cgagcacgcg tgcccaaggc aagagaacca tctgacccgg cataaagacg ccgagtgtca
     3361 tcagaccgaa cagaacttct gagccggcga agcgccactt cgacaaaaca tagccgttga
     3421 tggagccgat tgccgtcgag atggtggtcg ccggcacgac catcgcgacc gaattgaaga
     3481 aaaaaggctc tattccggcg caggtgccgc cgacgcagaa tttgtcccag gccaacgacc
     3541 agtaatcgag cgaaaagctg tgcggcagat tgatcaagcc gttgcggctg atgtccgaaa
     3601 gatcgcggaa ggaattgagg accacgacga ccagcggcag cagataatag agcgcgaaca
     3661 gcccgagcag cgtataaatg acgagttttg cccggtaacg ccgccaggtg ccagccgagc
     3721 caagcggatc ataggcgacg gtttcagcca cgcacgacct cccgacgctg gcgccaattg
     3781 gtccagattg tatagggcac cagcaccagt gacaacgcaa ggaagatcat gatcgccgcc
     3841 gctgcccctt gggccatctg gccccgttgg aacatcagat catagacata gattgccggt
     3901 accgtggtgg cgatccctgg cccgcctccc gtcaaggcaa tcaccagatc gaaagtttta
     3961 atggcaaatt gcagaagcac gaccaggaca gcgataaaga tcggcccgat cgccggcaga
     4021 atgatgcgcc gatagatccg cggcatatta gcgccgtcga tctgcgccgc cttgacgagg
     4081 tcgggatcga ccg
//
LOCUS       DATMPU010000178         3866 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_770349,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000178 DATMPU010000000
VERSION     DATMPU010000178.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 3866)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3866)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3866
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_770349"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(188..263)
                     /locus_tag="VNY06_04110"
     tRNA            complement(188..263)
                     /locus_tag="VNY06_04110"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(228..230),aa:Lys,seq:ttt)
     gene            complement(322..555)
                     /locus_tag="VNY06_04115"
     CDS             complement(322..555)
                     /locus_tag="VNY06_04115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016918203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1467 family protein"
                     /protein_id="HWX14037.1"
                     /translation="MPFSIPLAAATFMTIWFVVLFAVLPFGIRSQHEVGELTPGTDPG
                     APIAPKLLAKFAWTTLISAGVFAAVAALAKYAG"
     gene            complement(640..1020)
                     /locus_tag="VNY06_04120"
     CDS             complement(640..1020)
                     /locus_tag="VNY06_04120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012590093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF971 domain-containing protein"
                     /protein_id="HWX14038.1"
                     /translation="MRENQDRWPSEIRLRDNGHTLTVRFEGGECHDLSAEYLRVMSPS
                     AEVQGHRREDRLTIGGKRQCRITGIEPVGTYAVRLIFDDLHSTGLFTWDYLHELGFRH
                     KEKWALYEAELKAKGLSRDCPGEA"
     gene            1133..2194
                     /gene="moaA"
                     /locus_tag="VNY06_04125"
     CDS             1133..2194
                     /gene="moaA"
                     /locus_tag="VNY06_04125"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012384770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HWX14039.1"
                     /translation="MNIIVPVRRAGALPVATPLPAAPLTDPFGRRISYLRVSVTDRCD
                     MRCVYCMSEDMNFLPKRDLLTLGELDRLCTAFVSRGVSKIRITGGEPLVRRDIIRLFQ
                     NLSRHIHSGALNELTLTTNGSQLARHARQLAAASVRRVNISLDTLDPEKYRGITRGGN
                     HAQTLEGIDAAQEAGLSVKINMVALRGVNEDEIIPMLEWAHARGADLSLIEVMPLGAI
                     ETGRANQYLPLNIVKTRLKERFTLRELDYRTGGPARYVQVEETGGRLGFITPLTHNFC
                     ESCNRVRVTCTGTLYMCLGQEDAADLRTPLRASPADELLHQALEAAIARKPKGHNFLI
                     DSRHSHAPAVSRHMSVTGG"
     gene            2377..3237
                     /locus_tag="VNY06_04130"
     CDS             2377..3237
                     /locus_tag="VNY06_04130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012385236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HWX14040.1"
                     /translation="MKWQDFGRSANVEDRRGAGGMIGKGGLGIGTVAILGLLGWALGI
                     DPRLLIGGAEMVNKMTQGSQTGRVQTGTPADNMGQFVSAVLAANESVWTEVLPQQKGI
                     AYERPVLVLYSGRTVSGCGGAQAAMGPFYCPRDHKVYLDTTFFDDMRRRLGGGGDFAY
                     AYVIAHEIGHHVQNLLGILPKIQAAQQRAMSRGDANNLSVGAELMADCLAGVWAANAE
                     QKYRFLQPGDLESAVNTAQAIGDDRLQKSGQGYVVPDSFTHGSSAQRVQWLSVGLKSG
                     QVDSCNTFAR"
     gene            complement(3256..>3866)
                     /locus_tag="VNY06_04135"
     CDS             complement(3256..>3866)
                     /locus_tag="VNY06_04135"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HWX14041.1"
                     /translation="LKEIHHRVKNSLQLVSSMLNLHANSGVDSEVRTQLQEASNRVLA
                     IARAHESLYKDHDIGVLDVGSYLRDVCRDLSASASGCTVEIAAADGIRVATDRGISLA
                     LIAIELITNAAKYAYSGDSKGTIRVLLAKQEPDGLLLRVADSGAGLPRDFDLEKNTGL
                     GMRIVTALARTLDAKLNAKACNPGSEFELQIPLRSMNPAQLQ"
BASE COUNT          826 a         1111 c         1107 g          822 t
ORIGIN      
        1 catgcgtcct cgctgggcct caggcctgcg cgagaaacgc aagatttgca agcggctttg
       61 ggccgccttc ttggcgggta attttgctcg gacaacgaaa ttctggcgat gttgtccaga
      121 atgttgtcca ggaccaaatc agacttcaaa aaggctttaa gtattatcta taaccctttg
      181 gttttgctgg tgagcgcgcc gggaatcgaa cccgggacct cctgattaaa agtcagatgc
      241 tctaccagct gagctacgcg ctcccccaaa gggatggttt gcagtagccg cataggccgg
      301 aggggtcaat agaggtgacc gtcagccggc atatttcgca agagcagcta ctgcggcgaa
      361 aacacccgcc gaaatcagtg ttgtccaagc aaatttggca aggagttttg gggcgattgg
      421 cgcgccggga tcggtccccg gcgtcaactc tccgacctcg tgctgcgagc gaataccgaa
      481 cggaagcacc gcaaataaaa cgacaaacca aatcgtcatg aatgtcgcag ccgccagggg
      541 aatagaaaat ggcattttct ttttgcctct tcactttcct gttgcgtcat cggccaaaag
      601 gaggccaaag cagcagcaaa ggctcgacaa cgccgccgct cacgcttctc cggggcagtc
      661 gcggctcagc cctttggctt tgagctcggc ctcatagagt gcccatttct ccttatgcct
      721 gaacccaagc tcatgaagat agtcccaggt aaagagtccg gtcgaatgca aatcatcgaa
      781 gatcagccgc actgcatagg tgccgaccgg ctcgatcccg gtgatgcggc attggcgctt
      841 gccgccaatg gtcaggcggt cttccctccg gtggccctga acctctgcac tggggctcat
      901 gacccgtaaa tattctgcgg aaagatcgtg acattcgccg ccctcgaaac ggacagttag
      961 cgtatggcca ttgtctctca gccgaatttc gcttggccag cgatcctggt tttcacgcat
     1021 tgctgtggct acctggctag ctgcccccgc ctcgaagccc ctcattagtt taacgcatga
     1081 accctatgta tcactttggc gtagtatttc cgctcccgga tcggggaagc ctatgaatat
     1141 catagtacca gttcgccgcg ccggcgccct gcccgtggcc acgccgctcc cggcagcgcc
     1201 tcttaccgat ccgttcggtc gccgcatttc ctaccttcgt gtgtcagtga ccgaccgctg
     1261 cgatatgcgc tgcgtttact gcatgtccga agatatgaat ttcttgccaa agcgggactt
     1321 gctcacctta ggggagctgg atcgtctctg tacagctttc gtcagccgcg gcgtcagcaa
     1381 gattaggatc accgggggcg agcccttggt ccgccgcgat attatccgtt tgtttcaaaa
     1441 tttgtcgcgg cacatccact ctggtgcgct gaacgagctg acgctcacaa caaacggctc
     1501 tcagcttgca cgccacgcaa ggcaactcgc ggcagctagt gtgcgccgcg tcaacatatc
     1561 gctcgatacg ctcgaccccg aaaagtatcg gggaattacg cgcgggggca atcacgcgca
     1621 aaccttggag gggatcgacg ccgcccaaga agcggggctt tcggtcaaga tcaacatggt
     1681 ggcgcttagg ggcgtaaacg aggatgaaat catcccgatg ctggaatggg cgcatgcgcg
     1741 gggagcagac ctcagtctca tcgaagtcat gcctcttggc gcaattgaaa ccgggcgggc
     1801 gaatcaatat ttgcctttga acattgttaa aacacggctg aaggaaagat ttaccttgcg
     1861 cgagctggat tatcggacag gcggccccgc gcgttacgtg caagtcgaag aaaccggtgg
     1921 ccgcttaggt ttcatcaccc cgctcaccca caatttttgc gaaagttgca atcgcgtccg
     1981 cgtgacatgc accggaacac tttatatgtg cctcgggcag gaagacgcgg cagatctccg
     2041 caccccgctt cgcgcttccc ctgcggacga gttgttgcat caagccctcg aggccgccat
     2101 cgcccgcaaa ccgaaagggc ataatttcct aatcgacagc cggcatagcc acgcgcccgc
     2161 cgtttcgcgc catatgagcg ttaccggcgg ctaggctgcc cccaaagacg cattcgattc
     2221 tggctcaaaa ggtgctgtgg cgaccctttt ggttccaagc atttcgatgt aaaaatctac
     2281 aaatcgagcg tgttgttgtg gatgcgcttt gggcaggccc agagctcgca gcggtgcatg
     2341 cgagcgggaa actaggcaag caaacgggag cagcgcatga agtggcagga tttcgggcgt
     2401 tcggccaacg tcgaggaccg gcgcggagct ggcggcatga tcggcaaagg cgggcttggt
     2461 atcggcacgg ttgcgattct cgggctcctc ggctgggcgc tcggtatcga tccgagacta
     2521 ttgatcggcg gcgccgaaat ggtcaacaag atgactcagg gttcacaaac tggacgagtg
     2581 cagaccggca cgcccgccga caacatgggc caattcgtct cagccgtgct tgcggcaaac
     2641 gaatccgtct ggaccgaggt tttgccgcaa cagaaaggga tcgcctacga gaggccagtg
     2701 ctcgtcctct acagcgggag aacagtctcg ggctgcggtg gcgcacaggc agcaatgggg
     2761 cccttttatt gccctcggga ccataaggtc tatctcgaca ccaccttttt cgatgacatg
     2821 aggcggcgct taggtggcgg cggcgacttc gcctatgctt atgtgatcgc gcatgagatc
     2881 gggcatcatg ttcaaaacct cttggggatc ttgcctaaaa tccaggccgc gcagcagcgc
     2941 gccatgagcc gtggtgacgc gaataatctc tcggttggcg ccgagctaat ggctgattgc
     3001 cttgccggcg tctgggccgc aaacgccgaa caaaaatacc ggttcctgca gccaggcgac
     3061 cttgaatccg cggtcaacac cgcacaggcg attggcgatg atcgcttgca gaagtcagga
     3121 caaggctatg tcgtgcccga ttccttcacc catggctcat cggcacagcg cgtgcagtgg
     3181 ttaagtgtcg gattgaagtc gggccaggtt gattcttgca acacttttgc gcgttaggac
     3241 gcagacataa tctactcatt gaagttgcgc aggattcatg gacctcaagg ggatctgaag
     3301 ctcgaactcc gaccccggat tgcaggcctt cgcgttcagc ttagcgtcga gagtccgcgc
     3361 gagtgctgta acaatgcgca ttccaagacc cgtgttcttc tctaagtcga agtctcgagg
     3421 caggcccgcg ccgctgtcgg cgactctcag caacaaacca tccggttctt gcttggccag
     3481 aagaacccga atggtgccct tcgagtcacc cgagtaagca tatttggctg cattggtaat
     3541 aagctcgatg gcgatcaggg cgagtgaaat gccccgatcc gtcgcgacgc gaatgccgtc
     3601 ggcagcggcg atctcgaccg tacagccgga ggcagaagcg ctaaggtcgc ggcacacgtc
     3661 ccgcaagtaa ctaccgacgt cgaggacccc gatatcgtgg tccttgtaaa ggctctcgtg
     3721 cgcccgtgcg attgccaata ctctattgga cgcttcctgc agttgtgtcc ggacctccga
     3781 atcgacaccg ctgttcgcat gaagattgag catgctggag acaagttgca ggctgttctt
     3841 gacccggtga tggatttctt tgagca
//
LOCUS       DATMPU010000179         4231 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_1084020,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000179 DATMPU010000000
VERSION     DATMPU010000179.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 4231)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4231)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4231
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1084020"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..422)
                     /locus_tag="VNY06_04140"
     CDS             complement(<1..422)
                     /locus_tag="VNY06_04140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006022366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX14042.1"
                     /translation="MPYLHASAVVIGEAGVLIRGPSGSGKSALAYSLIAAAEESGYFA
                     RLVGDDRVRISSHGERLISHGHASILGKIERCSQGVIEVPFLAAAVLRLAVQLTSTDE
                     TALRYPEPDHDHILLAGSKLPCLSVRQDAAPAALSLAI"
     gene            complement(453..2303)
                     /locus_tag="VNY06_04145"
     CDS             complement(453..2303)
                     /locus_tag="VNY06_04145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020173389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HWX14043.1"
                     /translation="MSVEAREGTGFERAVGTGRKAKRVRRSFVLRQQAFTRAARARLS
                     SPLTRRIVVLNLGGLVALLFGFLYINQFREGLIDARVKSLQVQAEIIAAAIAASATVE
                     TDSIAIDPEKLLQLAPGQSYAPGEENVPSLEFSINPERIGPVLHRLASPTRTRARIYD
                     RDGYLLLDSQSLTSRSNIMRFDLAPPTNQEPSPLWTRLGNALSRFIARFGLPLYEDIG
                     VSNGKNYPEVAQALRGNAQSIVRVDAKGETIVSIAVPVQRFRAVRGALLLSTQGNDID
                     KIIASERWAMVRIFFVSSGIMFLLSFFIAGTIAEPIRQLAEAAERVRRGTRARREIPD
                     FTGRSDEIGHLSGALRDMTKALYDRIEAIERFAADVAHELKNPLTSLRSAVETLPVAR
                     TPESRQRLLAILAHDVRRLDRLISDISDASRLDAELARGDMDLVDLTRLLGAVVTVAN
                     MVKDNGAVRVELTIAHQGLPDTTNKPAFMVCGHDSRLGQVFNNLIDNARSFSPPEGGV
                     RVLLRPSRDYASPNQPRDGFEVIIDDDGPGIPANAFERIFERFYTDRPGQGFGQNSGL
                     GLSISRQIIEAHDGHIAALNRLGPPGDDGMPPVLGARFLVFLPKARKTIP"
     gene            complement(2597..3292)
                     /locus_tag="VNY06_04150"
     CDS             complement(2597..3292)
                     /locus_tag="VNY06_04150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012589305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HWX14044.1"
                     /translation="MPTIALVDDDRNILDSVAIALEGEGYRVQTYTDGSTALDGLKSA
                     PPDLAIFDIKMPRMDGMELLRRFRQKSDVPVIFLTSKDEEIDELFGLKMGADDFIRKP
                     FSQRLLVERVKAILRRATHKEPGVQKESEAKILERGPLKMDPERHSCTWKNDAVTLTV
                     TEFLILQALASRPGVVKSRNALMDAAYDDQVYVDDRTIDSHIKRLRKKFKAVDDEFEM
                     IETLYGVGYRFRE"
BASE COUNT          943 a         1225 c         1190 g          873 t
ORIGIN      
        1 aatatcgcga gtgagagagc cgcgggcgcg gcatcttgcc gcacgctcaa gcacggcagt
       61 ttggaacctg caagcaggat atgatcatgg tcaggctccg gataacggag tgccgtttca
      121 tccgtgctgg tgagctggac cgcaagccgc agaacggcgg cggccaaaaa cgggacctcg
      181 ataacgcctt ggctgcaccg ttctattttt cccaatatcg aggcatggcc atgggagatc
      241 agacgctcac catgcgaaga aattctgacg cggtcgtcgc ccacgagtct tgcgaaatag
      301 cctgactctt ccgctgccgc gattaggcta tacgcgagag cgctttttcc ggagccggag
      361 ggcccgcgaa tgagcacgcc ggcctccccg atcacgacag cacttgcatg gagataaggc
      421 aacttcgcaa tcccggccca ttggctttat ctctaaggga tggtctttcg ggctttcggc
      481 aggaagacca ggaatcttgc tccaagcacg ggaggcatgc cgtcatcacc gggtggtccg
      541 agacggttta aagcggctat atgtccgtca tgggcctcga taatttggcg ggagatcgaa
      601 aggcccaggc ctgaattttg gccaaatccc tggcccggac gatcggtata aaacctttca
      661 aatatccgct cgaacgcatt ggctggaatg ccggggccgt cgtcatcgat gatgacttcg
      721 aaaccgtcgc gcggctgatt gggcgaggca tagtcccgcg acgggcgcaa caacacgcga
      781 acaccgcctt cggggggcga aaacgatctc gcattgtcaa ttagattatt gaaaacctgg
      841 ccgaggcgcg agtcatgccc gcaaaccatg aaagcaggct tgttggtcgt atctgggagg
      901 ccttgatgtg cgatggttaa ttcaacccgc accgcgccgt tgtccttgac catgtttgcc
      961 acagtcacaa ctgcgccgag cagccgtgtc aaatccacga ggtccatgtc gccgcgcgcc
     1021 aactcggcat ctaaacgcga tgcgtcggat atgtcgctga tcagccggtc gagccggcga
     1081 acgtcatgag ccaaaatcgc caagagccgc tgacgcgatt ctggcgtacg tgcgactggc
     1141 agcgtctcga ccgcgctgcg caacgatgtc agcgggtttt tcaattcatg tgcgacatcg
     1201 gcggcgaagc gttcaatcgc ttcgatccgg tcgtagagtg cctttgtcat atcgcgcaag
     1261 gctccagaca gatggccgat ttcgtcggac cttcccgtga aatctggaat ttcccggcgc
     1321 gccctagtgc cccgccgtac tctttccgcc gcttccgcga gctgcctaat cggctcggct
     1381 atggtcccgg cgatgaagaa agacaaaaga aacataatgc ccgaggacac gaaaaatatc
     1441 cggaccatgg cccatctttc cgaggcgata atcttgtcga tgtcattgcc ttgggtggag
     1501 agaagcaagg cgccgcggac cgcccggaat cgctgcactg gcacagcaat agaaacaatg
     1561 gtctcgcctt ttgcgtcgac acggacgatg gattgcgcgt ttccgcgcaa ggcctgcgcc
     1621 acctccggat agttcttgcc gttgctgacg ccaatgtcct cataaagggg cagcccaaaa
     1681 cgcgcgataa acctgctcaa cgcattgcca aggcgtgtcc ataagggcga cggctcttgg
     1741 ttcgtcggcg gcgccaagtc aaaacgcatg atattcgaac ggctcgtcag cgactgggaa
     1801 tcgagcagca aatagccatc gcggtcgtag attcgtgccc gggtgcgggt gggagacgcg
     1861 agcctgtgaa ggaccgggcc gatacgttca ggattgatcg aaaactcaag cgagggcacg
     1921 ttttcttcgc cgggcgcata gctttggccc ggtgcaagtt gcagcagctt ttcgggatcg
     1981 atagcaattg agtcggtctc aactgtagcg gatgccgcta tcgccgcggc aataatctcc
     2041 gcttgaactt gcaaactctt gaccctagca tcgatcaagc cttcgcgaaa ctggttgatg
     2101 tagaggaagc caaagagtag cgccacgagt ccgccgaggt tcaacaccac aatgcggcgg
     2161 gtcagcgggg acgaaaggcg agcacgcgct gcacgcgtaa aagcttgctg ccgcaggaca
     2221 aaacttcggc ggacgcgttt ggccttgcgg ccagtgccta ccgcccgctc gaagcccgtt
     2281 ccttcccgcg cttcgacgct cattgatcag tgacccccaa acccacaacg tggagtctca
     2341 tgtcacagtc cgacacagtg aacggacccc ataactctgg gactcccaag cacacgcccg
     2401 tgcctttgta ccggacttcc gccccgcatg tagagcgagc catcctattg cgcggtacgc
     2461 ttgacttgtt tcgcaaaact tgcgctgcgc gatttaccgg gagaaagcct cccgtatagc
     2521 cgatagtgct ccagatcgcg gcaacgagac tagccatccg ccgtatctat gtgacggaat
     2581 tccggccggg acttgttcac tcccggaaac ggtagccgac accataaagc gtttcaatca
     2641 tttcgaactc atcgtcgaca gccttgaatt ttttgcgcaa ccttttgata tgactgtcga
     2701 ttgtgcgatc gtcgacataa acttgatcat cataggcagc gtccatcaag gcatttcggc
     2761 tcttgacgac acctggtctc gatgcgagcg cctggaggat cagaaattca gtcacggtca
     2821 aggttactgc atcgttcttc cacgtgcagg aatggcgctc cgggtccatc ttcaaagggc
     2881 cgcgctcgag gatcttggcc tccgattcct tttgcactcc gggttcctta tgggtagcac
     2941 ggcgcaaaat ggccttgacc cgctcaacga ggagccgttg cgaaaagggt ttgcgaataa
     3001 aatcatcggc gcccattttg agcccgaaca attcgtcgat ttcctcatcc ttcgacgtca
     3061 aaaagattac cggcacgtcg gatttttggc gaaacctgcg aagcaattcc atgccatcca
     3121 tgcgcggcat cttgatatcg aaaatcgcta gatcaggggg agcgctcttc aagccgtcga
     3181 gcgcggtgga tccatccgta taggtctgca cgcgatagcc ttctccttcg agcgcaatcg
     3241 ccacagagtc gaggatgtta cggtcgtcat cgactaaggc tatcgttggc atgaacgatc
     3301 ccagttttga agtttcgacg tcacattcca actccgcaaa gcgatcggga ataacccctc
     3361 caaaccgttg ccgaacagtt ggcttggttt gatggccttc cccggatgcc ggggatagaa
     3421 ggcggatttc tttccttctc cgagaaggaa agagggctct tcgcgcaaaa acccacccgg
     3481 ctggccgttc gagcgtcgta ctatgcggga caaccttaac tgtcatatca ggacaaaggc
     3541 gcggccgaaa taaggcaggg cgtggttttc accgcagcgt tcctccccgc ctggtccaag
     3601 ggataggagg gagctttcgc ttgaacctcc accggcaaca gtgatgtagc gaaatctagg
     3661 gcaagcgagg atcacattac ctcatactag tcgccggatt ttggctctgg cgactgacac
     3721 gccgacgcga tgtgacgacg gaccagctcc tacaaaaccc gactgaaatt ggacagattt
     3781 tggctcagag cgtgtccggc agaagcgcga accggttctg cggatgggtc agcgacaaga
     3841 cagggatctc gagcaaaatg acggctcgaa gaacggtcag ctgcctctag cggcggcctc
     3901 cccctgccag agctggggga cagggggtac gcctccccca caaggcaggc gcttcgcgcg
     3961 gctttcccgc ccttttttac cgagacatta catcggcccg ggatcgcctt tccgtatcga
     4021 cggccaggat agctggaatt ggaaagcggc ggaccacacc tcaggctgaa gaaaatggcg
     4081 aacgatcctt aggcggacac acaaccccat atgtgcgtca aattgacgca ccagtccccg
     4141 tcatgcgtct taagctgttc ctgtctatct ctgtccgcaa ggacaaacag tggataaaag
     4201 aacggagcgg ctctcaggcg tctctcgtgg g
//
LOCUS       DATMPU010000180         3394 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_3245652,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000180 DATMPU010000000
VERSION     DATMPU010000180.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 3394)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3394)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3394
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3245652"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..800)
                     /locus_tag="VNY06_04155"
     CDS             complement(<1..800)
                     /locus_tag="VNY06_04155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012383720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HWX14045.1"
                     /translation="MLQSFDMILKGGTVIDHTGMSPRDIGITGGKIAELGDLARASSG
                     ETINATGLHVLPGVIDSQVHFREPGLMHKEDLETGSRAAVLGGVTAVFEMPNTNPPTV
                     GAEELADKMARARGRMHCDYAFWVGGTADNWRDIPELERLPGSAGIKVFMGSSTGSLL
                     IAEDAGIAAILGQTKRRASFHSEDESRLNERQDFRARNDPSSHSIWRDEIVALRATER
                     LWRIARQKRAAIHVLHVSCGEEMDLLAAHKDIASVEVTPHHLTLSSED"
     gene            896..1726
                     /locus_tag="VNY06_04160"
     CDS             896..1726
                     /locus_tag="VNY06_04160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020176577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate-binding protein"
                     /protein_id="HWX14046.1"
                     /translation="MLCLLADRGVIEVKGADTAGFLQRLITNSVLDIPKGESRYAALL
                     TPQGKLMFDFFVVAHPHEPEAAYLIDCFKENIGELVKRLNLHKMRANIAIADKSEDFG
                     VAAILGESPPVGIEGIIYRDMRTPDMGLRVIAKHDTLRRIADVGEAAYEAHRIAQGVP
                     KGGADFAYGDAFAHEANLDLLNGVDFKKGCYVGQEVVARVEFRKSARKRIVRIHFDGR
                     APAQGTQIMADGVNIGQVGSAAGDEGLAMVRLDRFKEAQAAGLELKAGEVPVIISVPG
                     "
     gene            1737..2336
                     /locus_tag="VNY06_04165"
     CDS             1737..2336
                     /locus_tag="VNY06_04165"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020176585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008725 - DNA-3-methyladenine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HWX14047.1"
                     /translation="MPHQPAGDGKSRCPWPGTDPLYLAYHDEEWGVPERGDRALYEKL
                     MLDGFQAGLSWITILRKREAFRAAFDNFDPEKIARYDQRKIESLMKNSGIIRNRAKIE
                     GTIASARAWLRIQEAEGFSAYLWGFFDGKSVQNSFRDITEIPAQTPLSQRIAKDLKIR
                     GFNFCGPTVVYAFCQATGMVNDHLVSCWRHQVLSGVVET"
     gene            complement(2701..3354)
                     /locus_tag="VNY06_04170"
     CDS             complement(2701..3354)
                     /locus_tag="VNY06_04170"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWX14048.1"
                     /translation="MQLALFKVRTFRVSVIGGFITRLGVGGLPFLTPLLFQIGLGMPA
                     WQSGMMMMPTAAAAMGMKLISTRVLGLFGYRKVLIVNTVMIGLTICLYALVTARTSLA
                     AIIAIGLALGFFNSLQFSSMNSMAYADIETNESSMASTIASSFQQISMSFGLACGSLV
                     AAWYLGDLPQSDRLAVITALHRAFVTLGGLTIVSSLSFWTLRAGDGESVSRMAPDLA"
BASE COUNT          767 a          941 c          996 g          690 t
ORIGIN      
        1 taatcctcag agcttagggt caaatgatgc ggcgtcacct cgacgctcgc aatatcctta
       61 tgcgctgcaa gcaagtccat ctcctcgccg caggagacat ggagcacatg aattgcggcg
      121 cgtttttgcc gcgcgatgcg ccaaagccgc tcggtcgcgc gcagtgctac gatttcgtcg
      181 cgccagattg agtgcgagga aggatcgttg cgcgcgcgga aatcctggcg ctcgttaagc
      241 cgggactcat cctcgctgtg aaaggacgca cgcctttttg tctgcccaag gatggcggca
      301 atgccggcat cctcggcgat gaggagcgag ccagttgaag agcccatgaa cactttgatt
      361 ccagcggagc cgggcaggcg ctcgagttcc ggaatatcgc gccaattgtc ggccgttccg
      421 ccgacccaaa aagcgtagtc gcaatgcatg cggccccttg cccgcgccat tttatccgca
      481 agctcctccg cgcctactgt cggaggattg gtgttgggca tttcgaacac cgcggtaacc
      541 ccgccaagga ccgctgcccg agacccggtc tcgagatcct ccttgtgcat gagccctggc
      601 tcacggaaat gcacttggct atcaatcacg ccgggcaaaa catgcaggcc ggtggcattg
      661 attgtttcgc ctgagcttgc acgggctaga tcgccgagtt cggcaatctt gccacctgta
      721 ataccgatgt cgcgcggact catcccagta tgatcgatta cggtgccgcc cttcaatatc
      781 atgtcgaagc tttgcagcac tttctgaacc gtttgatagg atgtggcttg cgcccgggcg
      841 aggttcgatt atctacggcg atgcggcgct aaagtcgagg gattggatgg acggaatgct
      901 atgccttctt gccgatcgcg gtgtcattga agtgaagggc gcggatacgg ccggttttct
      961 gcagcggctc attaccaata gcgtgctcga tattcccaaa ggagagagcc gctatgccgc
     1021 gcttttgacg ccccagggca agttgatgtt tgatttcttc gttgttgcgc atccgcacga
     1081 accggaggca gcctatctca tcgattgttt taaggagaac attggcgaac tcgttaaacg
     1141 gctcaatctc cacaaaatgc gggcgaacat cgcaattgca gataaatccg aagattttgg
     1201 ggttgcggca atccttggcg aaagcccgcc ggtaggaatt gagggcatta tttatcgcga
     1261 catgaggaca ccggacatgg gtttgcgcgt catcgcgaaa catgacactt tgcggcgcat
     1321 cgccgatgtt ggcgaggcag cttatgaagc gcatcggatt gcgcagggcg tgcccaaggg
     1381 gggtgccgat tttgcctatg gcgacgcatt cgctcacgaa gcaaacctcg atcttttaaa
     1441 tggcgttgat ttcaaaaagg gatgctatgt cggccaggaa gttgtcgccc gcgtcgaatt
     1501 ccgtaaatcg gcgcgcaagc gcatcgtaag aatccatttt gatggaaggg ctccggccca
     1561 aggcacgcaa atcatggcgg acggggtaaa catcgggcag gttggctcgg ccgcgggtga
     1621 cgagggtctt gcgatggtgc ggctcgacag gtttaaggaa gcccaagcgg ctggcctcga
     1681 gctaaaggcc ggggaagtgc cggtcatcat ttcggtgccg ggctgaaagg gacgagatgc
     1741 cgcaccagcc agccggcgac ggcaaatctc gctgcccttg gcctggaacc gaccctctct
     1801 atcttgccta tcatgacgag gaatggggcg tgccggagcg tggtgaccga gcgctttacg
     1861 aaaaactgat gctcgacggt tttcaggccg ggctgtcctg gatcaccatt ttgcgcaagc
     1921 gcgaagcctt tagggccgcg ttcgacaatt tcgatccaga aaaaatcgcg cgttacgacc
     1981 agcgcaagat tgagtctttg atgaaaaact ctggaatcat ccgcaaccgg gcgaaaattg
     2041 aaggcacgat cgcatcggcg cgggcatggc ttaggattca agaggccgaa ggcttttcgg
     2101 cctatctttg gggctttttc gacggcaaaa gcgtgcaaaa tagttttcgc gacataaccg
     2161 aaatcccagc ccaaacgcct ctctctcagc gtattgccaa ggatctaaag atccgcggtt
     2221 tcaatttctg tgggccaacc gtcgtttatg ccttttgtca agcgacgggc atggtgaacg
     2281 atcatctcgt tagctgttgg cggcatcaag tgctttcagg agtcgtggaa acgtgaacgc
     2341 tctctggccg aatatgacgc cgagacccaa cctataaaaa gacgattccg atagagacag
     2401 cggccacctc gggcatcact gccagaataa ttgccgccct caaggatttt ccgctctggc
     2461 ttcttctagg attggcaatt tcgatgagca gtggagtgta caccgaaagg aaaatcgcgc
     2521 ggggaaaaat gcacgggccg taattttcat gctcgccgcg atagagtcca agcacatcgt
     2581 cgccgctgcg acaacttccg tggcggcgca acaagataag gcaagagatc agaaagctac
     2641 ctcgtcggcg tcaaactcgc ttagcaagaa tttggatgcg gtcaagcggc gcgggcggcg
     2701 tcatgccaga tccggcgcca tgcggctgac gctctcgcca tccccggcgc gcagcgtcca
     2761 aaaggacagc gaggagacga tggtcaaccc gcccaaggtc acgaaagcgc gatgcagcgc
     2821 cgtgatgacc gcgaggcgat cggactgcgg cagatctcca agataccatg ccgccaccag
     2881 cgatccgcag gcaagaccga agctcatgga gatttgctga aacgagctgg ctatcgtgct
     2941 cgccatgctc gactcattcg tctcgatatc tgcgtaggcc atgctgttca tgctggaaaa
     3001 ctgcagtgaa ttgaaaaacc ccagagcgag gccgatggct atgatcgccg ccagagacgt
     3061 gcgcgccgtc accagcgcgt agagacagat tgtcaggccg atcatcaccg tgttgacgat
     3121 cagcactttg cgatatccga aaagccccag cacgcgcgtg gaaataagct tcatgcccat
     3181 ggcggcggcg gcggtgggca tcatcatcat gcccgattgc cacgccggca taccaagccc
     3241 gatctgaaac agcaaaggcg tcaaaaaggg caatccgcca acgccgagcc gcgtgatgaa
     3301 gccgccgatc accgaaacgc gaaaggttcg caccttgaac agcgccagct gcaacagcgg
     3361 atagggcgtt tggcgcgcat gacgccaata ggcg
//
LOCUS       DATMPU010000181         2375 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_449962,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000181 DATMPU010000000
VERSION     DATMPU010000181.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 2375)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2375)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2375
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_449962"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            92..255
                     /locus_tag="VNY06_04175"
                     /pseudo
     CDS             92..255
                     /locus_tag="VNY06_04175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009490670.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="3D domain-containing protein"
     gene            252..860
                     /locus_tag="VNY06_04180"
     CDS             252..860
                     /locus_tag="VNY06_04180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012331309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HWX14049.1"
                     /translation="MNENFPFPTKPPRRRLSAAELELWLDATRSVVRRLGSPLPHYPQ
                     TKVEAELHRRAVTDQPSKGAGWEAASKEAPPKTRPTLAPIERRLRQKLSRGRITPDAE
                     IDLHGMRQREAFLALRAFLLRAQSDGARLVLVVTGKGNRAASGDFTPGILRKCVPNWL
                     CGADYHAIVAGFEEAARPHGGSGALYVRLRRRSQPATKKPIF"
     gene            920..1276
                     /locus_tag="VNY06_04185"
     CDS             920..1276
                     /locus_tag="VNY06_04185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012330834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase family protein"
                     /protein_id="HWX14050.1"
                     /translation="MAESAFAALPERFRRLCDDVVIRVEDFADDETLDALNCSSGFDL
                     LGLFRGRGRAQCGAAPVTGQFPNMVWLYRRPILEYWAQHENTLGDIVTHVLVHEIGHH
                     FGLSDEDMQRIEQTAE"
     gene            1416..2111
                     /locus_tag="VNY06_04190"
     CDS             1416..2111
                     /locus_tag="VNY06_04190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020177304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate transporter family protein"
                     /protein_id="HWX14051.1"
                     /translation="MLDAALAAARQILTPPFRRVLWKTLALTFALLALVWALLHKLIL
                     AGAALAHPWAATVLSFLSGVGLFICLAFLITPVSFIVAGFYSDELALVAEREFAPGEA
                     PGRALPLSESLWLSIKFSAVALAVNLAALLLMLVPGINVTAFFAANAYLSGRGYFELA
                     ASRHLPFGEVRRLRKINNLRLFGAGLFIAALLAVPIANLLTPLFGTAFMVRITRDILR
                     CARKTPHSALGSE"
     gene            complement(2116..>2375)
                     /locus_tag="VNY06_04195"
     CDS             complement(2116..>2375)
                     /locus_tag="VNY06_04195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020177303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="complex I NDUFA9 subunit family protein"
                     /protein_id="HWX14052.1"
                     /translation="KGATSIAFVTEAIHKLSFGLFPEIFMITSDQVKLLREDNIVSPL
                     ADVEGRTLHGFGISPEPYETLVPSYLYRYRKTGQFAGTRGA"
BASE COUNT          457 a          692 c          669 g          557 t
ORIGIN      
        1 gccaggaaat tgggcttagt gcattacgct ccattgccgt atctggagtt tccgcgaacc
       61 tgccttggta cagctcgcta ccgtcgccgt tcggcagctg atgatcgcgc aagacacggg
      121 ttcagctata actggccctg cccggtgcga tattttcttt ggttcaggga tgacgcgggc
      181 gcaccggcgg gcaatattcg gcacaaggcc gcgttctgcg ttcttctgcc ccgagacgag
      241 gctccagatt cgtgaatgaa aatttccctt ttccaaccaa gcctccacgc cggcggctga
      301 gtgcggcaga gcttgagctg tggcttgacg ctacacggag cgtcgtgcgc aggctcggta
      361 gccccttgcc tcactacccg cagaccaagg ttgaagcaga actgcatcgc cgcgcagtca
      421 ccgaccaacc ctctaaaggc gccggatggg aggcggcatc caaggaggca ccgccaaaga
      481 cgcggccgac ccttgcgcct atcgagcggc ggctacgtca aaagctttcg cgcggccgaa
      541 tcactcccga cgccgagatc gacctgcacg gcatgcgcca aagggaggcg ttcttggccc
      601 tgcgcgcgtt cctattgcgg gcccagagcg acggtgcgcg gctcgtcttg gtggtgaccg
      661 gcaagggcaa ccgcgccgca tccggcgatt tcacgccagg catactgcgc aaatgcgtgc
      721 cgaactggct ctgtggcgcg gactatcacg cgatcgtcgc cgggtttgaa gaggccgcac
      781 gcccacacgg tggctccggc gcactctatg tgcgtctcag acgccgcagc caaccagcga
      841 caaagaagcc aattttttga atacgcgcaa acaagagatt cataacctgg cggcgccttc
      901 gcttgctgac ttagagctca tggcggaatc ggcgttcgcc gcccttcccg aacgttttcg
      961 ccggctttgc gatgacgtag tgatccgcgt cgaggatttt gctgacgatg aaacgctcga
     1021 tgcgttgaac tgcagcagcg gttttgatct gttgggccta tttcgcggcc gtggccgcgc
     1081 gcagtgcggg gcagctcctg tgactgggca attccccaat atggtgtggc tttaccgccg
     1141 cccgatcctc gagtactggg cacagcatga aaacacactt ggcgacatag tcactcacgt
     1201 cctcgtccat gaaatcggtc atcatttcgg gctttctgac gaagacatgc agcggatcga
     1261 gcaaaccgct gagtagtccg caaggatcgt gatacgagcc tttttcccat caccccaggg
     1321 cgtgttgggc caaaagttag agccggtttt gcgtccggac acgcgacaat acaaagattt
     1381 agtcatcttg ctttagcatc gatgcggagt tcctaatgct tgatgccgcg cttgccgccg
     1441 cacgccagat tttgacgcca cctttccggc gggtgctttg gaaaacgctc gcactcacct
     1501 ttgctttgct tgctctcgtc tgggccttgc tccacaaact tatcttggcc ggcgctgcac
     1561 ttgcccatcc atgggctgcg acggtcttgt cattcctaag tggcgttggg cttttcatct
     1621 gccttgcgtt tctgatcact cctgtatcct ttattgttgc cggattttac tccgacgaac
     1681 ttgcgctcgt ggcggagcgc gagttcgctc cgggagaagc gcctggccgg gcgcttccgc
     1741 tcagcgaatc cttgtggctc tcaatcaaat tttccgctgt cgcactcgcc gtcaatcttg
     1801 ccgcgctttt gttgatgctt gttccaggta ttaacgtcac ggcattcttt gctgctaatg
     1861 cctatttgtc cgggcgcgga tatttcgaac ttgcagcctc acgacacctt ccatttggcg
     1921 aggtgcggcg gttgcgaaaa ataaacaatt tgcgtttgtt tggcgcgggg ctcttcattg
     1981 cggcgcttct tgccgtccca attgctaact tgcttacgcc gttgtttggc acggccttca
     2041 tggtgcgcat tacaagagac attctgcgat gtgctcggaa aacgcctcac tcggcattgg
     2101 gctcggaatg agctttcaag cgccacgggt gcctgcgaac tgaccggttt ttcgatacct
     2161 gtagagataa gagggcacga gcgtttcata gggttcaggc gaaatgccaa agccatgcag
     2221 cgtccgcccc tcgacatcgg ctaaaggcga cacgatattg tcttcgcgaa gcagcttaac
     2281 ctggtcagag gtgatcataa aaatttcagg aaaaaggccg aatgacaatt tgtggatagc
     2341 ttcggtaacg aaagcaattg aagttgcgcc cttga
//
LOCUS       DATMPU010000182         2430 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_2313397,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000182 DATMPU010000000
VERSION     DATMPU010000182.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 2430)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2430)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2430
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2313397"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(522..598)
                     /locus_tag="VNY06_04200"
     tRNA            complement(522..598)
                     /locus_tag="VNY06_04200"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(562..564),aa:Arg,seq:tcg)
     gene            complement(751..2004)
                     /locus_tag="VNY06_04205"
     CDS             complement(751..2004)
                     /locus_tag="VNY06_04205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003615086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HWX14053.1"
                     /translation="MIPVAIILAIVLSGALGISLIYRQAAAVAAHRERVPDGFAGWIS
                     AEEHRRAADYTLARTRLAIAETVFDTVLAVLWLIVLLGPLYALVAQFIAPGISRAVAV
                     VVAFALLGRVLHLPFSIYSTFALEAQFGFNRATPAMYLRDRIKGVALMALLGVPLLYG
                     LFWLLRVMPDTWWLLGWATFMALTIAMSVIYPAVIAPLFNKFTPMPDGALKSRVEALL
                     AKCGFASKGLYVMDASRRSTHGNAYFTGFGKAKRIVFFDTLLQNHTPDEILSILAHEL
                     GHYKLGHIGQRIAELAALTFVGFAALRWAFGSSGLAGAFGLPADPGVVLIVVLTAMEP
                     VLHLLSPLTSWLSRRAEYQADNFAKAMAGSGPMISALTKLARDNLSALTPDALYALFY
                     YSHPPVPARIARLRAEPALLDRIRI"
     gene            2134..>2430
                     /locus_tag="VNY06_04210"
     CDS             2134..>2430
                     /locus_tag="VNY06_04210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017482648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family membrane protein"
                     /protein_id="HWX14054.1"
                     /translation="MNLAPIAAAWQPRLLGLLRIVTALLLLQHGMAKLFGFPHVAMYD
                     NLRLLSLLGIAGVLELAGGILLLLGIFTRPVAFILSGEMAFAYFIAHAQKSFFPI"
BASE COUNT          547 a          704 c          723 g          456 t
ORIGIN      
        1 cgaagcccga acgaattgtt ggggtttgac ggtcgcccct cacgcagggg cgtggatcga
       61 aacgaggcaa aagccatgaa accgggaatg gaggacaagt cgcccctcac gcaggggcgt
      121 ggatcgaaac gccaacccga cgttcgaaac gatttgcggc ccaccaactc cacgtctcag
      181 catgggttgc aagcgcgacc atcggaccga agtggaaatt cagacacccg acggcggatt
      241 tcctctcata aaaacttcgg aattatgcac ttgacaagtt acgccagatt ggcctcctcc
      301 taatcggatg agaatgttgc tttttatgtt gccatttgtc cttttgaccg cctgcgaacg
      361 ggatgatccc gtgaagcgct gccaacggga atttggcacg caaggccaaa gtatcgtcga
      421 taaatgcgtg cgctgaggtg tcgcgccgcc gtcaggtaag cctgaaaaag atcaacctcg
      481 ggattgatgg cccgttgagg cttcttgact tcgtatccat atggccggcg cgacaggact
      541 tgaacctgca acctccagat tcgaagtctg gcgatctatc cagttgatct acgcaccgga
      601 atgaggatat ggcgcggttg gggcgggcat gccaagtata atatcgttcc cgtcgtcatt
      661 cgcgctcctg cggactcttg tatgcttcac agtttgaggg ttcgggcgag agtttttccc
      721 ggccgcacag ccctagataa gcgggcaccg ctagatcctt atccggtcaa gtagagccgg
      781 ctccgctctc agccgggcga tccgcgccgg aaccggcggg tgggaataat aaaacaaggc
      841 gtagagcgcg tccggcgtta gggctgaaag attatcccgc gcgagcttcg tcaaggcgct
      901 gatcatcggc ccggagccgg ccatcgcctt cgcgaaattg tcggcctgat actcggcgcg
      961 gcgcgacagc cagcttgtca gcggcgacag caaatgcaag acgggttcca tcgcggtcag
     1021 gacgacgatc aaaaccacac cgggatcggc cggcaggccg aacgcgccgg ccaatccgct
     1081 ggacccgaac gcccaacgaa gtgcagcaaa accaacaaac gtgagcgccg ccagctcggc
     1141 aatgcgctga ccgatatgac cgagtttata gtgccccagt tcatgggcga gaatcgacaa
     1201 aatttcatca ggcgtatggt tttgcaaaag cgtgtcgaag aagacgatgc gcttggcctt
     1261 tccgaagcca gtgaaatagg cgttgccgtg cgtcgatcgc cgggaggcat ccatgacgta
     1321 gagccccttg gatgcgaaac cgcatttggc aagaagcgct tcgacgcgcg acttcaacgc
     1381 tccgtccggc atgggcgtga acttattgaa caagggcgcg atgacggcgg gataaatcac
     1441 gctcatcgcg atcgtcagag ccataaacgt cgcccagccg aggagccacc aggtgtccgg
     1501 cattacgcgc agcagccaga acagaccata aagcaaaggg accccaagga gagccatgag
     1561 tgccacgcct tttattcggt cgcgcagata catcgccgga gtcgcgcggt tgaatccgaa
     1621 ctgggcttcc agcgcgaagg tgctgtaaat cgaaaaggga agatgaagaa cgcgcccgag
     1681 cagcgcgaaa gcaaccacga cggcgacagc gcgggagatg ccaggagcaa tgaattgcgc
     1741 caccaacgcg taaagaggtc cgagcaagac gatcagccaa agcaccgcga ggacggtgtc
     1801 gaagacggtc tccgcgatcg ccagccgcgt gcgggcgagg gtgtagtcgg ccgcccgccg
     1861 gtgttcctcg gcgctgatcc aaccggcaaa cccgtccgga acgcgctcgc gatgggcggc
     1921 aaccgcggcg gcctgacggt agattaggga gatgccaagc gcccctgaca ggacaatggc
     1981 caaaataatt gcgacgggga tcatctcctg attcctcgga tgccggcgga acttatatag
     2041 gataaaatac ctggcgtccg agtccaggga gcgcaccgct ctggcaatgg agaaacgccc
     2101 atgcgaggtt ctttcggcaa aggaatggtc atcatgaatt tggcgcccat cgccgctgct
     2161 tggcagccgc ggctccttgg acttttgcgg atcgtcacgg ccctgctgct tctccagcac
     2221 ggcatggcga agctattcgg ctttccgcat gtcgcaatgt acgacaacct tcggctcctg
     2281 tcgctcttgg gcatcgcggg cgttctggaa ctcgccggcg gcattctgct tctcctcggc
     2341 atcttcaccc gcccggtcgc tttcattttg tccggcgaga tggcgtttgc ctatttcatc
     2401 gcgcatgcgc aaaaaagctt ctttcccatt
//
LOCUS       DATMPU010000183         6670 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_451297,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000183 DATMPU010000000
VERSION     DATMPU010000183.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 6670)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6670)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6670
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_451297"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1142)
                     /gene="hflX"
                     /locus_tag="VNY06_04215"
     CDS             complement(<1..1142)
                     /gene="hflX"
                     /locus_tag="VNY06_04215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009491221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HWX14055.1"
                     /translation="MAKRESERRARALVIGPYSNGATSRRGGEGRSPAARLSEALGLA
                     QAIGLDVVSTQIVPLAQIRPASFLGKGKVDELASFVGVHDIELVVMDCALSPVQQRNV
                     EEAFAAKVIDRTGLILEIFGRRACTREGALQVELAHLAYQKSRLVRSWTHLERQRGGF
                     GFLGGPGETQIEADRRLIEERIVRIENDLERVKRMRSLHRQSRQAVLHPIVAMVGYTN
                     AGKSTLFNQMTEAGVVAEDKLFATLDPTSRGVKLPHGSQIILSDTVGFISDLPILLIK
                     AFRATLEEVVEADLILHVRDIAHPDSEAQRDDVETVLAGLGIDPANDRRLIEVWNKCD
                     FLGASDTFARNGGYRISQRKPILVSALTGQGIDGLAGAIENRLAGSR"
     gene            complement(1320..1580)
                     /gene="hfq"
                     /locus_tag="VNY06_04220"
     CDS             complement(1320..1580)
                     /gene="hfq"
                     /locus_tag="VNY06_04220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012384515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HWX14056.1"
                     /translation="MSADRPQNLQDTFLNFVRKNKVPLTIFLVNGVKLQGIVTWFDNF
                     CVLLRRDGHSQLVYKHAISTIMPGQPIQMFEPSDEAVGHLGQ"
     gene            complement(1759..2619)
                     /locus_tag="VNY06_04225"
     CDS             complement(1759..2619)
                     /locus_tag="VNY06_04225"
                     /EC_number="2.6.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012589576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino-acid transaminase"
                     /protein_id="HWX14057.1"
                     /translation="MSRVAYVNGRYVLHRSAIVPIEDRGYQFADGVYEVCEVFHGALI
                     DEKRHFERLAYSLAELQIAPPLRHGALAIVVREVMARNKVENGYIYVQVTRGVALRDH
                     IFPCPAVRPSLVVTGRQVDPEKGELIARKGVSVITTPDLRWKRVDIKSISLLPNVLAR
                     QLAKESGAYEAWFIDSSGMVTEGAASNAWIIGENGTIVTRHLDHSILRGITRTTLFEI
                     IAAQGLRLAERKFSLDEALAAQEAFITGATTLIMPVVCIDGKNIGSGIPGPTTLKLRA
                     IFHDIAARST"
     gene            2954..4294
                     /locus_tag="VNY06_04230"
     CDS             2954..4294
                     /locus_tag="VNY06_04230"
                     /EC_number="1.11.1.5"
                     /inference="COORDINATES: protein motif:HMM:NF015131.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HWX14058.1"
                     /translation="MQTSHGKLRASVAVTIIGGLLLAVVGLIFVSPESVPSVAPVALA
                     ADSIGKSVPAKPALPAGISSQIYALAVPPGREPTAQLVALGEILFNDKRLSVDDSVAC
                     ATCHDPKQGFTDHRGNAPTSAGVQNQHGQRNAPTVLNAMFQASQFWDGRAPTLEDQAK
                     LPPLNPVEMGQRTPDDVVAKVAKIQEYSNAFKSLLGRDFTYDDLAAAIAAFERTQYSG
                     NSPFDRYVAGDIQAISDSAKRGWALFQGKGRCSSCHAFNAVSPLFSDQKFHNIGIAAH
                     KQNFAELARKAFGILKSGDVKQIDELALQTSYSELGRFLVTKNPADIGAFKSETLRNI
                     GITAPYMHDGSLATLWDVMDHYNKGGVPNPFLDGGMQRLGLSEAEIDDLVAFLFTLTD
                     DRFADFNKAEMERQTSLKNNRPQRETEVAQGKKGDLGDAPLTPDLRNPATLGAF"
     gene            4415..5545
                     /locus_tag="VNY06_04235"
     CDS             4415..5545
                     /locus_tag="VNY06_04235"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HWX14059.1"
                     /translation="MTHKSIETKYYEERASFFEDLARMGQLSRRGFLHIASVSAGIAA
                     AKGLIVPQSFQLIEIAEAAETTTPAFTVAYISDTHIYTRDLNDRFVRSIIKAVEDVNA
                     LDPQPDFVLFGGDLAQLGRRQELDLGAQILKDLKAPVKMMVGEHDWFFDMGEHWQSLF
                     GPPQYSFDHKGIHFVTLMSVHEKDFWTARGMTPEERMQTVAGLDNGVQSRFEVGPEGR
                     QWLQDDLAKVDKKTPVVIFSHSPLYKYYRPWNFWTEDADEVQAILARFDTVSVIHGHT
                     HQLLVNHLGNISFYGLLATAWPWPYAPEGLPKLTVEMQMNRADPFNAFDGCGDGQLNL
                     LASGMIDKVYNLWNRNPVTVRASYLSSSGAKDRPPVATLTSY"
     gene            5557..6225
                     /locus_tag="VNY06_04240"
     CDS             5557..6225
                     /locus_tag="VNY06_04240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018408669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX14060.1"
                     /translation="MRLSRHFALALCLAAAPAIAADHLPRHKVPASASGNLVVGLCDG
                     ETSIEVKGVKPGDQLTREQGQQIADALMKEWRQKHRDSEWAAAAAEGGAPGQQPAPVA
                     TGQFQTYGAYTDRDRQIWKESTDRLVAEGKRIFHDDKALGGTNGISCDMCHPDASNTH
                     PETYPKFQTQLGRVALLRDMINWCIENPVRGKPFPDDDPKLKALEAYILAQRKGIALD
                     YGKH"
     gene            complement(6272..>6670)
                     /locus_tag="VNY06_04245"
     CDS             complement(6272..>6670)
                     /locus_tag="VNY06_04245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX14061.1"
                     /translation="ALAALFYFPLPLATHFRLIWVGPIITSAGINRGARDAEGRLRRL
                     ALLGGELSYAVYALHYPIFCWVNGLYQAAAGPRNLIIEGPVILASVLIGSYVAMKIYD
                     EPLRRRLGLAWQRARHSPSLAAPKRTSPPV"
BASE COUNT         1510 a         1910 c         1833 g         1417 t
ORIGIN      
        1 cggctgcccg cgagccggtt ttcgattgca ccggcaaggc cgtcgatgcc ctgccccgtc
       61 aaagccgaaa cgaggattgg cttgcgctgc gaaatccggt agccgccatt tcgcgcgaaa
      121 gtatccgatg ctcccaagaa atcgcatttg ttccatactt ctatcagccg gcgatcattt
      181 gcgggatcaa tgcccagacc agcgaggacc gtttcgacgt catccctctg ggcttccgaa
      241 tcaggatgcg caatatccct cacatgcaaa atgaggtctg cttcaactac ttcttcaagt
      301 gttgcgcgga atgccttgat tagtaagata ggaaggtcgg agataaaacc gacggtatcg
      361 gataggatga tttggctgcc atgcggcagc ttaacaccac gggaggttgg atcgagggtc
      421 gcgaaaagct tgtcttcggc gacgaccccc gcctctgtca tctgattgaa cagcgtcgac
      481 ttccccgcat tggtatagcc gaccatcgca acgatagggt ggagtacagc ctgccggctt
      541 tggcgatgca ggctgcgcat gcgtttgacc ctttcgagat cattttcgat ccggacaata
      601 cgctcctcaa tgagtcggcg gtcagcttcg atctgggttt cgccaggccc gccgaggaag
      661 ccaaatcctc cgcgctgacg ttcgagatgg gtccaagagc gaacgagccg cgacttttga
      721 taggcgagat gggcgagctc aacctgcagc gcaccttccc ttgtacaggc gcggcggcca
      781 aatatttcga ggattaggcc ggtgcgatcg atgaccttgg ccgcaaacgc ttcttcaacg
      841 ttacgctgtt gcaccgggga taaggcgcaa tccatgacga cgagctcgat atcatgcacc
      901 ccgacgaaag aggcgagctc gtcaaccttg cctttcccta gaaagctcgc aggacgaatt
      961 tgcgcaagcg ggacgatttg ggttgagaca acatctaagc cgatcgcttg tgcgagcccg
     1021 agcgcttcgg aaaggcgtgc cgcaggggaa cgcccctctc cgcccctgcg gctcgtagca
     1081 ccattgctat acgggccaat caccagggcc cgggcgcgcc tttcagactc gcgcttggcc
     1141 atttcgacct cggctagcct tcagcgcaaa tattttatct ttcgcaagga gccttatgcc
     1201 cgatccccga ccatcaattt gagagcacgc ctatatcagg accgctgccg tccagaaccc
     1261 cattatcgcc tagggccgtc atttgttcaa gaaccggttc caggcacgac tcgaaggctt
     1321 tattgtccaa gatggccgac cgcttcgtca ctaggttcaa acatctggat gggctgaccc
     1381 ggcatgattg tcgatatggc atgcttatag acgagctgag aatgcccatc gcggcgaagt
     1441 agcacgcaaa aattgtcaaa ccacgtgacg atgccttgga gtttcactcc attaacaagg
     1501 aagattgtga gaggaacttt gttcttacgg acaaaattga gaaatgtatc ctgaagattt
     1561 tgtggccggt ctgcactcat cgttcagaat ccttttttgt ttttggcgcc gaaacgacgc
     1621 atatcgttca ttatatcaat gataccgttg gcagcgatca tattagacca atagctggcg
     1681 cggcaagccc ctcaatgaac atatcgttaa gccctccgaa tcagttggaa aggtcgcgcg
     1741 ctcccacttg ccagagcatt atgtggagcg cgcggcaata tcatgaaata tagcgcgcaa
     1801 ttttaaggta gttggtccgg gaattcccga tccgatgttc ttcccgtcga tgcacacaac
     1861 tggcattatc aaggtcgttg ctccagttat aaaagcttcc tgagctgcca gtgcctcatc
     1921 caggctgaat tttcgttccg ccaacctgag accttgcgcc gcaataatct cgaacagtgt
     1981 tgtgcgggtg ataccgcgca aaatagaatg gtcgagatga cgcgtgacga tcgtcccatt
     2041 ctcgccgatg atccaggcat tggacgctgc gccttcggtg accatgcccg aagaatcgat
     2101 aaaccaggcc tcataagcac cgctttcctt tgcgagctgg cgcgcgagaa catttggcaa
     2161 gagagaaatt gatttgatat cgaccctttt ccaacgcagg tcgggagtgg tgataacgct
     2221 cacccctttt ctcgcaatta gttcaccttt ctccggatca acttgacgcc cggtgacgac
     2281 aaggcttggg cgcacggctg gacacggaaa gatatgatcg cgaagcgcca caccgcgcgt
     2341 cacttgcaca taaatgtagc cgttctcgac cttgtttctc gccatgactt cacgaaccac
     2401 aatggcaagg gcaccgtgtc gcaagggagg agcgatttgc agttctgcta gcgagtacgc
     2461 gagccgctcg aaatgccgct tttcgtcgat tagagccccg tgaaatactt cgcagacttc
     2521 atagacaccg tcagcaaact gatagccccg gtcttcgatg ggaacgatgg cggatcggtg
     2581 caagacgtac cggccgttca cataagccac tctgctcatg cgacgcgcgc ctccgacatt
     2641 ttcggattcg aaagggccgc cagccagcct gacgcggcta cgcggcaggc tgcatccttc
     2701 gtggcagcgc cagaccgcgg gaattttatg gaaacgtcat ccatgccctc tagtgtcttg
     2761 ccggtcgctg tggcaggcgt caagccgctt cttgcagcaa aacgcgcgcc gaagggccca
     2821 gcgaggcgtc cggctgtaga gaaattttgg tggttcacgt cccgcttttt ctggttatgc
     2881 tgactgcaaa aatgaattgt ccccgtttgc acattcacgt aagcttggca aaaaagagcg
     2941 tcgggaggat aagatgcaga cgtcgcacgg caagttgaga gcgagcgttg cggtcacaat
     3001 aattgggggc ctcttgcttg cggtggtcgg gctgattttc gtatcccccg agagcgttcc
     3061 gtccgtagcg cccgtcgccc ttgccgcgga ttccatcgga aaatctgtac ctgcaaagcc
     3121 tgccctgcct gccggcattt cctcccaaat ctacgcgctg gctgtaccgc cggggcgtga
     3181 gcccaccgcg cagctcgtgg cgctcggcga aatactgttc aacgacaagc ggctttcggt
     3241 cgacgattcg gttgcctgcg caacctgcca cgacccaaaa caagggttta ccgatcatcg
     3301 tggcaatgcg ccgacgtctg cgggcgttca aaaccagcat ggccagcgca atgcgccgac
     3361 ggtgttgaac gccatgtttc aggcatcgca gttctgggat ggccgcgcgc caaccctcga
     3421 ggatcaagcc aaacttccac ctcttaaccc ggtcgaaatg ggtcaaagaa caccagacga
     3481 tgtcgtcgcc aaggttgcca agatccagga atattcaaac gcgttcaaat cgctgttggg
     3541 gcgcgacttc acttacgacg atctagccgc cgcgatcgca gcctttgagc gcacgcaata
     3601 ttccggcaac tcccccttcg accgctacgt tgcgggagat attcaggcga tcagcgattc
     3661 ggcaaagcgc ggttgggcac tgttccaagg caagggacgc tgcagctcct gccacgcatt
     3721 caatgcggtt tcgccgctat tctcggatca gaagtttcac aatatcggca tcgccgcgca
     3781 taagcaaaac tttgccgagc ttgcccgcaa ggcgtttggc atcctgaagt ctggcgacgt
     3841 gaagcagatc gacgaactcg ccttgcaaac atcctattcc gaactcggcc ggtttttggt
     3901 caccaaaaat cccgccgaca tcggcgcctt caagagcgag actctgcgca acattggaat
     3961 cacggcgcca tatatgcacg atggttctct cgcgacgctc tgggacgtga tggaccatta
     4021 taataagggc ggcgttccaa acccgtttct tgacgggggc atgcagcggc ttggattgag
     4081 cgaagcagag atcgatgatc ttgttgcttt cctttttacc ctcaccgacg accgcttcgc
     4141 tgatttcaac aaggcggaga tggagcggca aacgtcgctc aaaaataacc ggcctcagcg
     4201 tgaaaccgaa gtcgcccagg gcaagaaagg agatctcggc gatgcccccc tcactcccga
     4261 tctgcgcaat ccggcgacgc tcggcgcttt ttagagcgtt agcttcgaag actcgcgagc
     4321 ccggttacgt caggtggcaa aacagaagcg gcaaaataga aagatggacg gtgaaaagtt
     4381 tcccacgaaa cattttcgcc cagggagcaa cgtcatgacg cataagagca tcgagaccaa
     4441 atattacgag gaacgcgcct cattcttcga ggaccttgcc cggatgggtc agctctcccg
     4501 gcgcggtttt cttcacatcg ctagcgtttc ggcggggatt gccgctgcga aagggctgat
     4561 cgtgccgcaa agctttcagc ttatcgagat tgccgaagcg gcggagacca caacaccagc
     4621 ctttaccgtt gcctatatct ccgacacgca catttacacg cgcgatttga atgaccgttt
     4681 cgtgcgctcc atcataaaag cggtggaaga tgtcaacgcg ctcgatcccc agccagattt
     4741 tgtgctgttc ggtggtgatc tggcccagct cggccgcagg caggagcttg atcttggcgc
     4801 ccaaattctc aaggatttga aggccccggt gaaaatgatg gtcggcgagc acgactggtt
     4861 ctttgatatg ggcgagcact ggcagtcctt gttcgggcca ccgcaatatt cgttcgatca
     4921 caagggcatt catttcgtga ccttaatgag cgtccacgaa aaagattttt ggaccgcacg
     4981 tggcatgacg ccagaggaga gaatgcagac cgtcgctggg ctagacaacg gcgtccagtc
     5041 gcgcttcgag gttggaccgg aagggcgtca atggctgcaa gacgatctcg ccaaggtcga
     5101 caaaaagacg ccagtcgtta tcttctcgca ctcgcctctt tacaaatatt atcgcccttg
     5161 gaatttctgg acggaggacg ccgatgaagt ccaggcgatt cttgccagat tcgatacggt
     5221 gtccgtaatt cacggccaca cccatcagct cctcgtgaac cacctcggaa acatcagctt
     5281 ctacgggctg ctcgctaccg cctggccgtg gccctatgcc cccgagggac tgccgaagct
     5341 taccgtcgag atgcagatga accgcgccga tcccttcaac gccttcgacg gctgcggtga
     5401 cggccagctc aacctgctgg cctctggcat gatcgataag gtctataatc tttggaaccg
     5461 caatcccgtg accgtgcggg caagctatct ttcgtcgagc ggcgccaagg atcgcccgcc
     5521 cgtggcaacg cttacgagct actgagggag acgtagatgc gactttcccg ccacttcgca
     5581 ctcgcacttt gtctcgcggc agcccccgcg atcgcggcgg atcatcttcc ccgccataag
     5641 gtgcctgctt ccgccagtgg caatttggtt gttggccttt gcgatggcga aacctcgatc
     5701 gaggttaaag gtgtgaagcc cggcgatcaa ctgactcgtg agcagggaca acaaatcgcc
     5761 gacgcgctta tgaaagaatg gcggcaaaag catcgtgata gtgaatgggc cgccgccgcc
     5821 gctgagggcg gagcgccggg ccagcaaccg gcccctgtgg ccaccggtca attccaaacc
     5881 tatggcgcct ataccgatcg cgaccgccag atttggaaag agtcgactga tcgcttggtg
     5941 gcggaaggaa aacgcatttt tcatgatgac aaggcacttg gcgggaccaa cggcatatct
     6001 tgcgacatgt gccatccgga cgcctccaac acccaccccg aaacttatcc gaaatttcag
     6061 acccagctcg ggcgcgtcgc gctgcttcgc gacatgatca attggtgcat cgaaaatccg
     6121 gtacgtggca agcctttccc agacgacgac ccgaagctca aggctctcga agcctatatc
     6181 ctcgctcaaa ggaagggaat tgcgctagac tatggcaagc attgaccagc aggatgctgc
     6241 gttgagaatg cacgcaatgg tgatttgggc actagactgg cgggctcgtt ctcttcgggg
     6301 cggctaaaga gggagaatgg cgagcgcgct gccaggcaag gccgagacgg cggcgtagcg
     6361 gttcgtcgta aatcttcatt gccacatagc tgccaattaa aacgcttgcc aatatgacgg
     6421 ggccttcaat gataaggttc cgcggtcctg cggcggcttg gtaaagaccg ttgacccagc
     6481 agaatatcgg gtagtgaaga gcatagaccg cataggagag ttcgccgccc aaaagtgcga
     6541 gccgtcgcaa tcgtccttca gcgtcacgcg cgcccctgtt aataccagca gaggtgataa
     6601 tagggccgac ccaaatcaaa cgaaaatgcg tagcaagagg gagaggaaag tagaacagcg
     6661 ccgccagcgc
//
LOCUS       DATMPU010000184         4883 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_2016468,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000184 DATMPU010000000
VERSION     DATMPU010000184.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 4883)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4883)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4883
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2016468"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..189
                     /locus_tag="VNY06_04250"
     CDS             <1..189
                     /locus_tag="VNY06_04250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX14062.1"
                     /translation="AVIDSNSTANLNVLNSGGAVVLSKSTLTGSSIAISNSGGSVISY
                     GNNLIRAAGSPNSTVPLQ"
     gene            complement(353..574)
                     /locus_tag="VNY06_04255"
     CDS             complement(353..574)
                     /locus_tag="VNY06_04255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX14063.1"
                     /translation="MTTPRALSSGSSSGANAGTTEASETTITLGTTYVDRFQTDAIAS
                     PNIPRLMPFHFIRRHGRRATARPLTSIIG"
     gene            complement(923..2104)
                     /locus_tag="VNY06_04260"
     CDS             complement(923..2104)
                     /locus_tag="VNY06_04260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019904926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase"
                     /protein_id="HWX14064.1"
                     /translation="MFPACFAFCLPLFAATGACAASFSACLGDLRGAALASGVDSATF
                     DKATQGLTPNPDVLAMAEVQPEFKTPIWDYLAGLVDEERVNDGRAMMARWSQALSAAE
                     SRFGVDKAIITAVWGVESDFGRSFGTRPVIQSLATLACSGNRRAGYFRSELIAALKII
                     QHGDVDPAQFNGSWAGAFGHTQFMPSTFLKSAVDLDGDGRKDLINSVPDALGSTAAYL
                     HKSGWLKGVEWGFEVLLPAHYAGPSGRGRKEARAAWAARGLTRIDGTPLGPGPNAGLF
                     LPAGPNGPAFLVTRNFDAIYSYNAAESYALAIAILSDRLRGRHGIVAAWPTSDPGLSR
                     AERREMQTLLMRRGYDLDGKADGVIGTKTKQAISDFQSKSGLHPDGRASVSVLAALRG
                     H"
     gene            complement(2173..2556)
                     /locus_tag="VNY06_04265"
     CDS             complement(2173..2556)
                     /locus_tag="VNY06_04265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016920543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWX14065.1"
                     /translation="MNESPNAINSKILLAEDDHDMRRFLSKALQAAGFNVASFDNGKS
                     AYQRLREEPFELLLTDIVMPEMDGIELARKATELDPDIKVMFITGFAAVALNPDNNAP
                     RQAKVLSKPFHLKELVNEVQRLLAA"
     gene            2696..3727
                     /locus_tag="VNY06_04270"
     CDS             2696..3727
                     /locus_tag="VNY06_04270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012386543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-formylglutamate amidohydrolase"
                     /protein_id="HWX14066.1"
                     /translation="MSYLDWVASLWPGGGRFRYRTGERFKLFANSLQDVVLKGYEPHE
                     PEFAPPFEVVEPARLTCPLVFSSPHSGNVYPRRFLAAARLEEAVLRRSEDAFVDVLFG
                     GVNALGAPLIKARFPRAYLDLNREPYELDPRMFEGTLPPFANTRSLRVAGGLGTIPRV
                     AAQVQEIYVRRMPVEEAMLRIEKLYKPYHACLAKLIERARRVFGIAVLVDCHSMPSAI
                     RRADHTPLFGDLNPPGKPDVVIGDRFGKSCGTDLVDALEGELRRGGYIVQRNKPYAGG
                     YITEHYGNPANDCHAMQIEVSRALYMDECGVRRSERFAAVAEGVTKAAAVLVEAVAAR
                     HGRLRAAAE"
     gene            4244..4702
                     /locus_tag="VNY06_04275"
     CDS             4244..4702
                     /locus_tag="VNY06_04275"
                     /inference="COORDINATES: protein motif:HMM:NF028481.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0001401 - SAM complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tom37 metaxin N-terminal-like domain-containing
                     protein"
                     /protein_id="HWX14067.1"
                     /translation="MLRLFSYPDLYGVADNNPYGLKIYAFLKLSKLTFQHEHILRYKS
                     APRGQLPYLVNGEKTVGDSDAIISHLITRYALTIDDDLTANQRDTDLLVRRMLEDLYW
                     VMSYSRWADPRFRPLFRKALLTTHPDITEAALEAAREYNFTRYHYQGIGR"
BASE COUNT         1010 a         1376 c         1434 g         1063 t
ORIGIN      
        1 gccgttatag attcaaattc aacggcgaat ctcaacgttc tcaacagcgg cggcgcagtg
       61 gtgctttcga aatcgaccct cactggaagc agtattgcta tctcgaacag tggtggctcg
      121 gtcatttctt acggtaataa tctgatccgc gctgccgggt cgccgaactc gaccgtgccg
      181 ctgcagtaat atataactcg gattgagacg gccgctgata gatactgctc ccgctaccgg
      241 gcgctatgcc tgttccggat ggcacttcat catggccagc gtgcgagtat tcactcggca
      301 ccgatagaga taacagggct aagcagcgcg agcacgccca agaggcgcgg cgtcagccga
      361 taatggatgt caacggtcgc gcggtcgcgc ggcgaccatg cctcctgatg aaatgaaatg
      421 gcatgaggcg ggggatattc ggtgacgcaa tggcgtctgt ttggaagcgg tccacgtagg
      481 tggtgccgag tgtgatcgtg gtctccgacg cctcggttgt gccggcattc gcgccggacg
      541 aagacccgct cgatagggct cggggtgtcg tcatagtctg cggtggccca gattagcagg
      601 gatcgcagca aactcaactt agcgatggac acaccccaga ttcgctgagt tcgcgcagga
      661 aatcgagatg tccgttcttg gcgcagagca gaaattcgat ctctgctctg aaggccgagc
      721 cgtgccggtc gtcccgctgt cttaacgcac gccaacggtg tttcaagctc tggcggccaa
      781 ttctaccaaa gttccgcgca atcccacttt cccatgccgc tgtgtatgcg gccttgtaag
      841 gtttcgggtt atttcgggac aaaatgtttc acgtgaaaac attttgtccg attgccggtc
      901 actttcggac aaaagttttg ggtcagtggc cgcgaagcgc ggcgagcacg gagacagagg
      961 cacggccatc agggtgcagg ccggattttg actgaaaatc ggagatcgcc tgtttggtct
     1021 tggtcccgat cacgccatcc gccttgccgt cgagatcata gccgcgccgc atgagcagcg
     1081 tctgcatctc cctgcgctcc gcgcgggaaa ggccaggatc gctggttggc caggccgcaa
     1141 cgatcccatg ccgcccgcgc agccgatcgg acaaaatcgc aattgccagc gcgtaggatt
     1201 ctgctgcgtt gtaggaatag atcgcatcga aattgcgggt gacgagaaac gccggaccat
     1261 tggggccagc aggcagaaac agacccgcat ttggcccggg gccaagcgga gttccgtcga
     1321 tccgggttag gccgcgcgcg gcccaggccg ccctcgcttc cttacggccg cgcccggacg
     1381 ggccggcata atgcgccggc agcaggacct caaagcccca ttcgacgcct ttcaaccagc
     1441 cgctcttgtg caaataggcg gcggtcgagc cgagcgcgtc ggggacggaa ttgatcaaat
     1501 ccttgcggcc atccccatcg agatcgacgg cacttttcaa aaatgtcgag ggcatgaatt
     1561 gggtgtgccc aaaagcgccc gcccaggaac cgttgaattg ggcaggatcg acatccccat
     1621 gctgtatgat ctttagcgcg gcgatgagtt ctgaccgaaa atatcccgcg cgccgattgc
     1681 ccgagcatgc gagcgttgca agcgactgaa tgaccgggcg cgttccaaag ctgcggccaa
     1741 aatccgattc gacgccccac acggcggtaa ttatggcctt gtcgacgcca aatcggcttt
     1801 cggcggcact cagcgcctgg ctccagcgcg ccatcatcgc ccggccgtcg ttgacgcgtt
     1861 cctcgtcaac gaggcctgca agatagtccc atatcggtgt cttgaactcg ggctggacct
     1921 ccgccatcgc cagcacatcg ggattggggg taagcccctg agtggcttta tcgaaagtcg
     1981 cgctgtcgac gccgctggcg agcgccgctc cgcgcaggtc gccaaggcag gccgaaaagc
     2041 ttgcggcaca ggcgccggtc gcagcgaaaa ggggaaggca gaaggcaaag caagcaggaa
     2101 acaggcgcaa agcgcgggcg gcgtttttgg ccaaggcgtt acctcatctg tgaaacgcca
     2161 agcatggcgc gatcaggcgg caagaaggcg ctgaacctcg tttacgagct ccttgagatg
     2221 aaaaggtttc gacaacacct tggcctgtcg cggcgcatta ttgtccgggt tgagtgcgac
     2281 cgccgcaaag ccggtgataa acatcacctt gatgtcgggg tcgagttcgg tcgccttccg
     2341 cgccagttcg ataccgtcca tctccggcat gacaatatcg gtcagcagca attcgaacgg
     2401 ttcctcgcgt aatctttggt aggcggactt gccattgtcg aaggaagcga cgttaaagcc
     2461 cgcggcctgc agcgctttgc tgagaaagcg gcgcatgtca tgatcatcct ccgccaggag
     2521 aatcttggaa ttgattgcgt tcggcgactc gttcatattc atggggtgcc tcgtcaacta
     2581 atgccataag aacgcgggcc gggtaaaaat cgagtgaaaa atgactgaat ttgcattatc
     2641 cgttaatttt ttcgcatcag gcaagtggct ctcaggtaga tctggataga tatttatgtc
     2701 ctatttggac tgggttgctt ccctttggcc tgggggcggg aggtttaggt atcgaacggg
     2761 cgagaggttt aagctctttg caaattccct gcaggatgtc gttttgaaag gctacgagcc
     2821 gcatgagcct gaattcgccc ctccatttga ggttgtggaa cccgcgcgcc tcacctgtcc
     2881 cttagtcttt tcttctccgc actcgggcaa tgtctatccg cgccggtttc ttgcggcggc
     2941 gcggcttgag gaagctgtgc ttcgccgttc ggaagatgct tttgtcgatg tcttgtttgg
     3001 cggcgtgaac gcgctcggcg caccgcttat taaggcgcgc tttccccgcg cctatcttga
     3061 tcttaatcgc gagccctatg agctcgatcc ccgcatgttc gagggaacgc tgccgccttt
     3121 tgccaataca aggtcactgc gtgtcgcggg cgggcttggg accattcctc gagttgcggc
     3181 gcaggtgcag gagatttatg tccgccgcat gccagtcgaa gaggctatgc tacgaatcga
     3241 gaagctctac aagccttacc acgcttgcct tgccaagcta atcgaaagag cccggcgcgt
     3301 tttcggtatc gccgttctcg tcgactgcca ttccatgcct tcggcgatcc ggcgcgccga
     3361 ccacacgccg ctttttggtg atttgaaccc gccgggcaag cccgatgtcg tcatcggcga
     3421 tcgttttggc aaaagttgcg gtacagatct tgtcgatgcc ctcgaaggag agctccgccg
     3481 cggcggttat attgtgcagc gcaataagcc ctatgcgggc ggttatatta cggaacatta
     3541 tggcaacccc gcgaatgatt gccatgcgat gcaaatcgag gtgagccgcg ctctctatat
     3601 ggatgagtgc ggtgtgcgca ggagcgagag attcgccgcg gttgcggagg gtgtaaccaa
     3661 ggccgccgcc gtcctggtgg aggcggtagc cgcgcgccac ggccggctaa gggcggcagc
     3721 tgagtaggca gcgtattatc cgagggcggg cccgctggaa cttgggcggg acgggctcac
     3781 gctgcataaa aggggcggcc gaaacgaaag aggccgcttg tgttgccaca agcggcccaa
     3841 gtctagggag gaaacgccca aggagggcat cggcggcaaa agtcttgccg cgtcgcaaca
     3901 acatacacgt gcgacgcaca aaaatcaaga gggtcaagtg atttttgtgt gaattttatc
     3961 attcgtataa ttaattgttt ttattttgtt attattgaca gcgcaagcca agcaggagga
     4021 atggaccaat ttttccacca aattctgatg tgccagctgt ccgaaacggc gtagccgctg
     4081 ccgcatattt tcacctcgaa cgagaaggcg ttcgtgcgct tgcgccgcat ggtttcgctt
     4141 cgtatcctcg accggatcgg actggctggc cgaacgcatt tccgattttc tgcgacaacg
     4201 gcctgcggta cgcgcccacc gcggagttat gagttacgcc cccatgctga ggttgttctc
     4261 ttacccggac ctttatggcg ttgcagacaa caacccctac ggcctgaaaa tctacgcttt
     4321 tctgaaattg tccaagctta cctttcagca tgaacatatc cttcgataca aaagcgctcc
     4381 gcgtggtcag ctgccttatc tcgtcaacgg cgaaaagacg gttggcgaca gcgacgcgat
     4441 tatctcacat ttgatcacgc gatatgccct caccatcgac gatgacctga cggcaaacca
     4501 gcgcgacacc gatcttttgg taagacggat gctcgaggat ctttactggg tcatgtccta
     4561 ttcgcgctgg gccgaccccc gcttcaggcc gcttttccgg aaagcccttc tgacaacaca
     4621 tcccgacatt accgaagccg ccttggaagc ggcacgggag tacaatttta cgcgttacca
     4681 ctatcaaggt attggacgct aggagcccga ggcagtttac gccagaggta tcgcgggacc
     4741 taagagtcct ggcgggactt gttcctggca gcggttttcc attcgggggc cggccagcca
     4801 gcatcgatgc gggcatttat ggatttacgg ccaatatgta ttttcgacac ccctttgaag
     4861 aactttctcc tatcgcggcc aaa
//
LOCUS       DATMPU010000185         5674 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_2943179,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000185 DATMPU010000000
VERSION     DATMPU010000185.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 5674)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5674)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5674
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2943179"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(50..613)
                     /locus_tag="VNY06_04280"
     CDS             complement(50..613)
                     /locus_tag="VNY06_04280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008836323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF447 domain-containing protein"
                     /protein_id="HWX14068.1"
                     /translation="MALIREVIVSTIDRSSRAQLAPIGLIEDGQGWIIAPFRPSKTAD
                     NLAETPFAIANYTDDASIFAGLITGRHDWPLAAAKKIPIPRLASALAHAELGVTRVEE
                     DAERPRFHCEVLHCEQHAPFAGFNRARNAILECAILVTRMHILPREKIDREIDYLAIA
                     VEKTAGPREREAWSWLMQKREAYYAAN"
     gene            complement(676..2064)
                     /locus_tag="VNY06_04285"
     CDS             complement(676..2064)
                     /locus_tag="VNY06_04285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014890532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6513 domain-containing protein"
                     /protein_id="HWX14069.1"
                     /translation="MSERLLLLTGHLAYRRLERLMRALGQTSFSWEICDIGVKVAALM
                     TETIIRHRLRRPIEADRILLPGRCRADPARLSNDLGVPVTRGPDELSDLPVFLGHGGI
                     TPDLTHFAIRIFGEIVDATALSVEALLTRAAALRKSGADVIDLGCLPDTPFPHLEKSI
                     RALKAAGHPVSVDSADRQELARGANAGADFLLSLDESTLEIAEVGGATPILVPAPHGD
                     LESLVRAVRRAEELGLSFIVDPILDPIHFGFANSVARYVETRRRLPNAEMMMGTGNLT
                     ELTEADSCGVTAVLIGLCSELDIRNVLTVQVSPHTRRTIEEHDAARRLMFAAREDSAL
                     PKGYNAGLLQLHDRAPFPSSVQDLAEIAAQVRDANFRIAVAEDGIHIFNRDGHYIARD
                     ALSLFPKLRVESNGAHAFYLGAELAKAEVAFALGKRYAQDEPLDWGCGSEKQEEDKNR
                     LREAGHTLRKNA"
     gene            complement(2155..2790)
                     /locus_tag="VNY06_04290"
     CDS             complement(2155..2790)
                     /locus_tag="VNY06_04290"
                     /inference="COORDINATES: protein motif:HMM:NF012899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX14070.1"
                     /translation="MLLSSPLSKLTIVKLGGSLAHGPQCLAWLDILAEWGGPLILVPG
                     GGPFADCVRAAQGAMGFADSTAHRLALIAMGQFGLALAAHSDAFTAAASPDELDRALV
                     RGKIPVWLPEKLVLEVQQVPASWEVTSDSLAAWLAGTFGACRLLLIKSCDLSAPSSAG
                     TLAARNIVDPAFPRFVAEAQVEVWFAGPESLAGAASILQGGGMPGSRITHT"
     gene            complement(2780..3946)
                     /locus_tag="VNY06_04295"
     CDS             complement(2780..3946)
                     /locus_tag="VNY06_04295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012591395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(5-formylfuran-3-yl)methyl phosphate synthase"
                     /protein_id="HWX14071.1"
                     /translation="MTLMLASVTSPAEAEAVFAGGADIIDLKDPAKGALGALDIGVAA
                     DIVRSIGRRRPVSAAAGAAQRPPREVVDTVAAIAATGVDYVKVGISPEGVAADCVRAL
                     SGHAKKSKLVGVLFADCTPNLDILALMADNGFAGAMLDTAKKGAGRLLDHLDIAALDR
                     FVDRCRKRGLLSGLAGSLEAPDVPRLLPLKPDYLGFRGSLCKGRIREAAIDAASVKMI
                     RELIPRESGGQSWRDEGTIDWRLFLARGMVTPDDRFVETDRIFVHDLVLPCAIGAYDF
                     ERTGTQDVRFNIDVDVRRMEHRFDDMRGVFSYDVIIDAIKIILGRGHVDLIETLASGI
                     ADEILRYPCAVRVCVRVEKLDIVCGTVGIEIKRESTAEHAGAGEILPVFPGNAA"
     gene            complement(3968..4261)
                     /locus_tag="VNY06_04300"
     CDS             complement(3968..4261)
                     /locus_tag="VNY06_04300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX14072.1"
                     /translation="MDFACVQVLFADLQQGIIARSRTNPPEELASSAGVLAEVAKLLR
                     LPIHFSLEAAGGVTTSVVGLVTALVPDFSTNPGQQAFGALQSLRHRPNTALNI"
     gene            complement(4320..4982)
                     /locus_tag="VNY06_04305"
     CDS             complement(4320..4982)
                     /locus_tag="VNY06_04305"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HWX14073.1"
                     /translation="MRAITAHASGAAKTVQEASADEDSSWIEDGLDGFGGETAMPYPV
                     LIVGAGPTGLTVALDLSRLDIPVRLIDKQREAATTSRAIGVQARTLELLEQRGLAEEM
                     VHLGNPGLAGSVYGGGQRMFRLDFGLVDSHYAYVCFISQAETERILRDAIGRQGVTIE
                     RGVELVGLSQDALTHDPSPVKAVLRHADGLLELAQAPWLISAEGAHSVARTTLDLPAR
                     LQ"
     gene            complement(5354..5662)
                     /locus_tag="VNY06_04310"
     CDS             complement(5354..5662)
                     /locus_tag="VNY06_04310"
                     /inference="COORDINATES: protein motif:HMM:NF016367.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(5-formylfuran-3-yl)methyl phosphate synthase"
                     /protein_id="HWX14074.1"
                     /translation="MLASVMSPAEAEAVFAGGADIIDLKDPARGALGALDIGVGAALS
                     EAILLNTRGSIAGASVANGFAVREGRLETLGRRLARHHASTRAIARKKSGIGRSRTLA
                     "
BASE COUNT         1225 a         1729 c         1639 g         1081 t
ORIGIN      
        1 aggcctgcga ttgcgcccct gcctcggctg ttgcccttct cgccgccgct cagtttgcgg
       61 cgtaataggc ttcccgcttt tgcatgagcc aagaccaggc ctcgcgctct cgggggccgg
      121 ccgttttttc gacagcaatc gcaagataat cgatctcccg gtcgattttc tcgcgcggca
      181 ggatatgcat tcgggtcacg agaatggcgc attccagaat cgcgttgcga gcacggttga
      241 atcccgcaaa gggcgcgtgc tgctcgcaat gcaacacctc acaatggaaa cgtggacgct
      301 cggcatcctc ctcgacccgg gtgacaccaa gctcagcatg ggcaagcgcc gaggcgaggc
      361 gagggatcgg aatttttttt gcagctgcga gcggccagtc atgccgcccg gttatgaggc
      421 cagcgaagat agaggcgtca tcggtgtaat tagcaatggc aaagggagtt tccgcaagat
      481 tgtcggccgt tttcgaggga cgaaatggcg cgatgatcca tccttgacca tcctcgatga
      541 gaccaatagg ggcaagttgc gccctgcttg accgatcgat ggtcgaaaca atgacttcgc
      601 ggattaaggc cactttgtat tctcctcaaa gcgattcatg aatggcgcaa tggctgttcc
      661 gctgccggca ccaaatcacg catttttgcg tagcgtgtgg cccgcctcgc gcaagcgatt
      721 cttgtcctct tcctgtttct cactgccgca gccccagtcg agaggttcat cttgcgcgta
      781 gcgtttgccg agcgcaaagg caacttccgc cttggcaagt tcggccccca gatagaaggc
      841 gtgcgcgcca ttgctctcaa ctcttagctt gggaaataag gacaaggcat cgcgcgcgat
      901 ataatggcca tcgcggttga aaatgtggat gccatcctcg gctaccgcga ttcgaaaatt
      961 tgcgtcccgc acttgggcag cgatttcggc aaggtcttgc accgacgaag gaaaaggcgc
     1021 gcggtcatgg agctggagga gaccggcatt ataacccttt ggtaaggcgc tgtcttcgcg
     1081 cgccgcgaac atgagacggc gcgccgcgtc atgttcttcg attgtacgcc gcgtatgagg
     1141 gcttacttga actgtcaaca cgttgcgaat gtcgagctcc gagcaaagcc cgatcaggac
     1201 cgcggtgacg ccgcaagaat ccgcctcggt caattcggtg aggtttccgg ttcccatcat
     1261 catttccgcg tttggaagac ggcgcctcgt ctcgacataa cgagcgacag agttcgcgaa
     1321 tccgaagtgg attgggtcaa ggattggatc gacaataaaa gaaaggccaa gctcttcggc
     1381 ccttcggacg gcacggacta gggattcgag gtctccatgc ggtgcaggta caaggatggg
     1441 tgtcgcgccg ccgacctccg caatttcaag agtgctttcg tcaagactaa ggagaaaatc
     1501 cgccccggca ttcgcaccgc gcgcaagttc ctgccggtct gcggaatcaa cgctgacggg
     1561 atgcccggcc gctttcaatg cgcggatgct cttttcgaga tgcggaaaag gcgtgtctgg
     1621 aaggcatcca aggtcaatga catcggcgcc ggatttccgc aatgcggctg cgcgcgtgag
     1681 taaagcctca acggaaagcg cagtagcatc gacgatctcg ccgaagatgc gtatggcaaa
     1741 atgcgtgaga tcaggcgtta taccgccgtg gccaagaaac actggcaagt cgctgagctc
     1801 atccggcccg cgcgtgaccg gtacgccgag gtcattcgat agacgcgcag gatcggcgcg
     1861 gcatcggcca ggaagtaaaa tgcgatcggc ctctatcggc ctccgaaggc ggtgcctgat
     1921 gatcgtttcg gtcatcaagg ccgcaacctt gacgccgata tcgcaaatct cccaactaaa
     1981 cgacgtctgg cctaaggccc gcatcagcct ttcgaggcgc cggtaagcga gatggccggt
     2041 gagaagaagg agacgctcgc tcatgtcagg aaactacgcg cgacactttt gaacttctct
     2101 tggcccacaa atccatggct ggacctattt catagtctag gagcggagat cacatcacgt
     2161 atgcgtaatc ctgctgcccg gcattcctcc accttgcaat atggacgctg ccccggcaag
     2221 cgattcggga cccgcaaacc aaacctcgac ttgagcttcc gccacgaagc gtgggaacgc
     2281 aggatcaaca atatttctgg ctgcaagggt gccagcggac gaaggagcgc taagatcgca
     2341 cgatttaatg agcagcaacc ggcaagcacc aaaggtcccg gcgagccatg ccgcgagcga
     2401 atctgaggtt acctcccaag acgcaggcac ctgctgcact tctaacacca gcttctccgg
     2461 caaccagacg ggaatttttc ctcggactaa cgcgcgatca agctcatcgg ggctcgccgc
     2521 agcagtgaat gcgtcagaat gcgcggcaag tgcgaggcca aattgcccca tagcgatgag
     2581 cgccaggcga tgtgcggtcg aatcggcaaa acccatcgcg ccttgggctg cgcgcacgca
     2641 atccgcaaat ggaccgccgc ctggaacgag gatgagcggg ccaccccact ctgccaatat
     2701 gtcaagccag gccaaacatt gcggcccatg cgcaagactg ccccctaatt taacaatggt
     2761 aagcttcgat aaaggcgagc taagcagcat tacctggaaa caccggcaag atttcgccag
     2821 cgccagcatg ctcggccgtg ctttcgcgct taatctcaat accaacggtg ccgcacacaa
     2881 tatccagctt ttcaacacgg acacaaacgc gcacggcgca ggggtagcgc aagatttcgt
     2941 ccgcgatgcc gcttgccaag gtttcgatca aatcgacatg tcctcgcccg agaatgattt
     3001 tgatagcgtc gataatcaca tcgtaggaga aaaccccacg catgtcgtca aatcgatgtt
     3061 ccatgcgccg cacatccaca tcgatgttaa agcgcacatc ctgcgtcccg gtgcgctcaa
     3121 aatcataggc gccgatcgca caggggagca cgagatcatg cacgaaaata cggtctgtct
     3181 cgacgaagcg gtcatcggga gtgaccatgc ctcgtgctaa gaaaagccgc caatcgattg
     3241 tcccttcgtc acgccaagat tgcccaccac tttcgcgcgg tatgagctca cggatcatct
     3301 tcacggacgc cgcatcgatt gccgcctccc gtatccgccc tttgcaaagg ctgccgcgaa
     3361 accccaaata atctggcttg agaggtagaa ggcgtggcac gtccggcgct tcaagggaac
     3421 cggcaagacc cgacagtaat ccgcgcttgc ggcatcgatc aacaaagcga tcgagggctg
     3481 caatgtccaa atgatcgagg agacgtcctg cgcctttttt ggcggtgtca agcatcgcgc
     3541 cggcaaagcc gttgtcggcc ataagagcca ggatgtcgag attgggcgtg caatcggcaa
     3601 aaagaacacc gaccaatttg cttttctttg catgcccgct cagcgcccgc acgcaatctg
     3661 ctgccactcc ctctggcgaa attccaactt tgacgtaatc gaccccggtt gcggcgatcg
     3721 cggccacggt gtcaacaacc tcccgcggcg ggcgttgtgc agcacccgcg gcggcgctaa
     3781 ccggcctgcg tctgccgatg gaccggacaa tgtccgcagc aaccccaata tcaagcgcac
     3841 cgagcgcgcc tttcgccggg tctttgagat cgatgatgtc ggcgcccccg gcgaaaaccg
     3901 cctccgcctc ggctgggctg gtcacactcg caagcatcaa ggtcattatc ccattcctca
     3961 ggtaccatta tatgttgagt gccgtgttcg ggcgatgcct tagcgattgc agggcgccaa
     4021 aggcttgctg tccgggattg gtgctgaaat caggcacgag cgcggttaca agccccacga
     4081 ccgacgtggt tacgccgccg gccgcctcga ggctgaagtg gatcggcagc cggagcagct
     4141 tggcaacctc ggccagcacg ccagcggagc tggcaagctc ctccggcgga ttagtcctgc
     4201 ttcgcgcgat gatgccctgt tgcaggtcag cgaacaggac ctggacgcag gcgaagtcca
     4261 tcagcgatcg tcccccgcgt cagccggcat gggcgtgcgg ctccggcgtg agccactgtt
     4321 cattgtaggc gcgcaggcag gtcgagcgtg gtgcgggcca cgctatgggc gccttcggcg
     4381 ctgatcaacc acggcgcctg cgccagttca aggagcccgt cggcgtggcg caacaccgcc
     4441 ttgaccggag aaggatcatg ggtaagtgcg tcctgtgata gcccgactag ctccacgccg
     4501 cgctcgatgg tcacgccctg ccggccgatc gcatcacgca ggatacgctc tgtttcggcc
     4561 tgcgagatga agcagacata ggcgtaatgg ctgtccacaa ggccgaagtc cagccggaac
     4621 atgcgctgtc cgccaccgta cacgctgccc gccaggccag gattgccaag gtggaccatc
     4681 tcctcggcga ggccacgctg ctccagcaac tccaatgtcc gcgcctggac gccgatggcg
     4741 cgcgaggtgg tagccgcctc gcgctgcttg tcaatgagcc gaacagggat gtcgaggcgg
     4801 gacaggtcca atgcgacagt caggccggtc ggcccggcgc ccacgataag gactgggtag
     4861 ggcatggcag tctctcctcc aaaaccatct aagccgtcct cgatccagga cgagtcctcg
     4921 tcggccgagg cttcttgcac ggtcttcgcc gcacccgagg catgcgcggt aatcgcgcgc
     4981 aacttatgag cggccaccag cttaagtacg gcgcgcgaag cgcagcccgt ggaattttta
     5041 caagtgtgga gccctcgcgt aactcaactg gcggccagcg tggcctccgc gccggggcca
     5101 aaaagcacgc ctgcgccgca tctgcgggaa ttttagcgcc cgaatatagg gattggaaac
     5161 ggaaaagcaa ttcatgctcg cggcgggcgc gcggctagtc acgctgcgtg aaacctcgtt
     5221 gcaatgagca cgcaagttcc aaggcgctgc cagcgcgacc accaagcggc ttgccattgc
     5281 attctttgac cgcgcttacc cggctaatgc tgttggtgat aaaatctcat ccgtcgcggc
     5341 aaggtcttca acattaagcg agtgttcgac tgcgaccaat cccagatttt ttgcgagcaa
     5401 tagcacgagt gctcgcatgg tgcctggcga ggcgccgtcc gagggtttcc aaccgccctt
     5461 cccgaactgc gaaaccattc gcgacggagg caccagcaat gcttccacgt gtattgagca
     5521 gaattgcttc ggacaatgcc gcaccaaccc caatatcaag cgcaccaagc gcgcctctcg
     5581 ccggatcttt cagatcgatg atgtcagcgc ctccggcgaa aaccgcttcc gcctcggctg
     5641 ggctcatcac gctcgcgagc atcagattga tacg
//
LOCUS       DATMPU010000186         6447 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_3249020,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000186 DATMPU010000000
VERSION     DATMPU010000186.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 6447)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6447)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6447
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3249020"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..189)
                     /locus_tag="VNY06_04315"
     CDS             complement(<1..189)
                     /locus_tag="VNY06_04315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007768615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HWX14075.1"
                     /translation="MIYDFAIIGAGIVGLAAAREILHARPGSSIIVLEKESTVAQHQT
                     GHNSGVIHAGIYYAPGSLK"
     gene            352..801
                     /locus_tag="VNY06_04320"
     CDS             352..801
                     /locus_tag="VNY06_04320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012115042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1772 domain-containing protein"
                     /protein_id="HWX14076.1"
                     /translation="MLAGELALVIASLFTGAAVYVHVAEQPARLRLDDASLLAEWKPA
                     YKRGFAMQASLALIGFILGVVAAYIQSEWRWLPGAFVLLANWPFTLIAIMPVNNRLMA
                     TKAEDAGTESRELIRQWGRLHAVRSLLGATSTALFLWASLSNDLSRE"
     gene            complement(790..1566)
                     /gene="xth"
                     /locus_tag="VNY06_04325"
     CDS             complement(790..1566)
                     /gene="xth"
                     /locus_tag="VNY06_04325"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012384399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008311 - double-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HWX14077.1"
                     /translation="MIIATWNVNSVRQRTEHLLRYLREVRPDVLCLQELKCVEEAFPF
                     SDAEAEGYNVAVHGQKGFNGVAILARAPIEVAKGLPGDPNDEQARYLEAMIPNGAGIV
                     RVASIYLPNGNPPQTEKYAYKLAFMDRLIAHAKYLLTLEEPLVLAGDYNVIPTPHDCA
                     DPAAWEGDALFLPETRARFRCLLNLGLTDALRATTDDSGLYTFWDYQAGAWQRNKGIR
                     IDHLLLSPRAADRLVELTIDKERRGEDKPSDHVPVRMSLA"
     gene            complement(1563..2858)
                     /locus_tag="VNY06_04330"
     CDS             complement(1563..2858)
                     /locus_tag="VNY06_04330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020175688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HWX14078.1"
                     /translation="MSFPTPEMQADVLVAGAGAAGLSTAIALAQAGFSVFCAGPIDRR
                     PNGRTVALFEASLRFYKSLGVWQRFRGKTAPLAKITMINATGPRFKVPTLSIAAAEIG
                     LSAFGENVENNVLADGLAHIASTTPNLVLHEGMISDICFGDETVCATLANGHRVTALL
                     VAAADGRMSLARTKAGIGTQSWTYPQIAYTALLSHAKPHRNVSTEFHTRSGPCTLVPL
                     RPIEGKPNRSSLVWLMSTEAAEHRRSLPETDLAREIEEQVDSLLGTMEIDGPGGFFPM
                     VGMKANRLACHRVALIGEAAHIFPPLAAQGLNLSLRDAAALAEIVEDARVLGADLGAA
                     QTLKTYEGARRSDVFIRTNGVDILNRSLLMNNFPVEFLRSAGVIAFSMIGPLRRALMR
                     EGVLPHGNLPRLMRERPVWRYLPFTREAKTEAPERGIHV"
     gene            3104..5206
                     /locus_tag="VNY06_04335"
     CDS             3104..5206
                     /locus_tag="VNY06_04335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012386380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX14079.1"
                     /translation="MSQFGISISPLLPWPLLIGFALAAFGLIALGFFARARGTLLRAL
                     GLGLLLLAMLGPGLVREERNPLKTVVAVVVDRSGSQSIGERPEQTDKARAELEKNLNA
                     LGNVETRVIESSQSDSEADGTRLFSALSAGLADVPLQRVGSVFMITDGEVDDIPADPG
                     ALGFKAPLHALITGHDGERDRRIELLEAPRFGIVGKEQTIELKVNDTADTGDPVILNV
                     RRDGNPIARMATAPGERIRLPVKIEHGGPTVIELEVEALPDELTAINNKAVLTIEGVR
                     DKLKVLLVSGEPHAGERMWRNLLKSDPNVDLVHFTILRPPEKQDGTPINELSLIAFPT
                     SDLFGRKIKEFDLIVFDRYSNQSVLPLIYFDNIVRYVREGGALLLAAGPDFARPEGLY
                     YSPLGRIAPARPEGGLAEHAFRAAITEDGAKHPVTRGLPGADQAPPAWSEWFRQINAE
                     VTHGTAIMSGAAGKPLLVVSREGKGRVALLLTDQMWLWARGFEGGGPTLDLLRRLAHW
                     LMKEPDLEEEALRANVRGRSIEIERQSLKGDSPQVTVTAPKGATESIPLKAAEPGISR
                     ATVDAAEMGLYRISDGSLSVLANVGPENPREFQNVVSTLDKLKPIAESTGGTVRRIAN
                     GKNGITVPRLVAIGDSPVYGGAGYAAIKRSGASELKGVSMAPLAIGFLGLAALLGSVL
                     LAWVFEGHNRSWSRRTRG"
     gene            complement(5227..5766)
                     /locus_tag="VNY06_04340"
     CDS             complement(5227..5766)
                     /locus_tag="VNY06_04340"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020186357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HWX14080.1"
                     /translation="MADLPLTLSPQTPADLAAIEKLDERAFGPGRFARSAYRLREGIE
                     PDYGLSFVARVGTLLVGAIRMTPIFCGDRPALLLGPLTVDPPFRSSGIGEELVKTSLN
                     AARVAGHELVLLAGDMSYYSKIGFLPVPSKRLIFAGPVDPQRLLYCELVEGAFEGVTG
                     KVRRASQESKPTPSKANHA"
     gene            6014..>6447
                     /locus_tag="VNY06_04345"
     CDS             6014..>6447
                     /locus_tag="VNY06_04345"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HWX14081.1"
                     /translation="MNVPTGTDIHQAVDLLRAGKKEECRGCVRALYSGGETNHVTCNL
                     AGLVSLSLDEYLMALQWFDRALALYPAYLDALANRGVALQQAGRHPEALADYDEAIRW
                     GCSKPELFYNCGNILRESGRLAEAIASYDIALRLEPAYPEALR"
BASE COUNT         1362 a         1809 c         1953 g         1323 t
ORIGIN      
        1 cttcaaactt cctggcgcat aatagatacc ggcatggatg accccgctgt tatgcccggt
       61 ttggtgttgt gcgacagtgc tttccttttc aagaacaatg atcgaggagc cggggcgcgc
      121 gtgcagaatt tcccgtgcgg ccgcgaggcc gacgatacca gcgccaatga ttgcaaaatc
      181 atagatcatt cggagatagg caatcacgcg tacggagttt aattgcggca accccccttc
      241 gccgcggaca tttccattcc gagtggatgc aatctaatag atggcttaca gctggagacg
      301 caagaccgcg acaacgaatt ggcacatact catttcaagg gagaaatagg catgcttgct
      361 ggagaactcg ccctcgtcat cgcgtctctt tttaccggcg ccgccgtcta cgtccatgta
      421 gcagagcagc cggcgaggct tagacttgac gacgcctcgc tgcttgcaga atggaaacct
      481 gcctacaagc gtggttttgc gatgcaggct tccttggcgc tcattggctt tatcctgggt
      541 gtcgtagcgg cctatattca atccgagtgg cgctggcttc caggcgcatt cgtgcttctc
      601 gccaactggc cattcacgct catcgccatc atgccggtga acaaccgcct catggcgacg
      661 aaagccgaag acgcagggac cgaaagcagg gagttgatcc ggcaatgggg gaggttgcat
      721 gcggtacgga gcttgcttgg cgctacttcg acggcccttt tcctttgggc ctcgttgtca
      781 aacgatctgt cacgcgagtg acatccgcac aggaacgtga tcggaaggct tatcctcgcc
      841 acgcctttct ttgtcgattg tgagctcgac caatcgatcg gctgcgcgcg gcgaaagcaa
      901 aagatggtca atgcggattc ctttgttgcg ttgccaagcg ccggcttgat agtcccaaaa
      961 agtgtagagc ccagagtcgt cagtggtggc gcgcagggcg tccgttaggc caagatttag
     1021 caggcaccga aagcgtgctc gcgtctcggg cagaaaaagc gcgtccccct cccatgcggc
     1081 gggatcggca caatcgtggg gggttggaat cacattatag tctccggcaa gaacaagcgg
     1141 ctcctccagc gtcaaaagat attttgcgtg ggcgataagg cgatccatga aggcgagttt
     1201 atacgcgtac ttctcggtct gcggcggatt gccgttcggc agataaatcg aggccacgcg
     1261 gacaattcca gcgccattcg ggatcatcgc ttcgagatag cgcgcttgct cgtcgtttgg
     1321 atcgccagga agtccctttg ccacttcgat cggcgccctg gcgaggatcg cgacgccatt
     1381 aaaacccttt tgcccatgca ccgcgacatt atagccttcg gcttctgcgt cgctgaaagg
     1441 aaatgcctcc tcgacgcatt tcagctcctg gagacagaga acatcaggtc ttacttcgcg
     1501 caaatagcgc aagagatgtt cggtccgctg gcgtaccgaa ttgacgttcc aggtggcaat
     1561 gatcaaacgt ggattcctcg ctccggcgcc tcggttttgg cttcgcgcgt gaaaggcaaa
     1621 tagcgccaga ctggtcgctc ccgcatgagg cgcggcaaat tgccatgggg gagcacgccc
     1681 tcacgcatga gggcgcgtcg caacgggccg atcatcgaaa acgcaatcac cccggcacta
     1741 cgcaagaact cgactggaaa attattcata agcagggagc gattgaggat gtcgacgccg
     1801 ttggtgcgta taaaaacgtc gctccggcgg gcgccctcgt aggttttcag tgtctgagcg
     1861 gcgccgaggt ccgctcctaa cacgcgcgca tcctcgacaa tttcggcaag ggcggcggca
     1921 tcccggaggc tgaggttcag gccttgggcg gcaagcggcg gaaagatatg cgcggcttcg
     1981 ccaatgagtg cgacgcgatg gcacgcgaga cgattcgcct tcatgccgac cattggaaaa
     2041 aagccgcctg gcccatcgat ctccattgtg ccaagcagag agtcgacctg ctcttcgatc
     2101 tctcgtgcca aatctgtctc aggaagtgaa cgccggtgct ccgccgcctc ggtggacatc
     2161 agccaaacga ggcttgacct gttcggcttg ccttcgatgg ggcgaagcgg aacgagcgtg
     2221 caggggccgg accgcgtgtg gaattcggtc gagacgttgc ggtgcggttt tgcgtgcgac
     2281 aaaagcgcgg tataggcaat ctgcggatat gtccagcttt gcgtgccgat cccagctttg
     2341 gttctcgcaa gcgacatgcg tccatcggcc gcggcgacga gaagcgccgt gactctgtgc
     2401 ccgttggcga gcgtggcaca gaccgtttcg tcgccgaagc aaatgtcgct gatcatgccc
     2461 tcgtgcagaa cgaggttcgg cgtggtcgag gcaatatggg caaggccatc cgcgaggacg
     2521 ttattttcga cgttctcgcc aaaagcggaa aggccgattt cggctgcggc gatggagagg
     2581 gtgggaacct taaaccgtgg tccggtagca ttgatcatgg tgatctttgc gagcggcgcg
     2641 gtcttgcccc gaaagcgttg ccaaacgcca agggacttat aaaagcggag cgacgcttca
     2701 aacagggcaa cggtccggcc attggggcgg cggtcgatag gaccagcgca aaaaactgaa
     2761 aatcctgcct gcgccaaggc aatagcggtg gaaaggccgg ccgcacccgc gccggcaaca
     2821 agaacatcag cttgcatttc cggagttggg aatgacattg agcacctatg cgcgtctatt
     2881 tagaccagag ttttagacca gagttgcaga tttggcgagt tgagggcaat ttgagcgtcg
     2941 gaggcacccg ggacaatcgt gagcactgcc tttgaagccg gacattgcca atattgacgc
     3001 gaagtgtaag agactgggcg tgccgtcgat agccgagcgc tgggcggcaa atcggcgggt
     3061 aacgcttttg ccgtgactac agccggaaag cttaaaagcc tcgatgagcc agttcggtat
     3121 ctccatttcc cccttgctac cctggccgct tctgattggc tttgccttgg ccgcgtttgg
     3181 gctcattgcc cttggctttt tcgcgcgggc gagagggact cttctccgcg cgttgggcct
     3241 cggcttgctg ttactcgcga tgctggggcc cggattggtt cgagaagagc gcaacccgct
     3301 aaagacagtc gttgccgttg ttgtcgacag aagcggcagt cagtcaattg gtgaacgccc
     3361 cgagcaaaca gacaaggccc gcgcggaact cgaaaaaaac cttaacgccc ttggtaatgt
     3421 cgagactcgg gtaatcgaga gcagccaaag tgatagcgaa gccgatggaa cgaggctgtt
     3481 ttccgcactg agcgctgggt tggcggatgt gcctttgcaa cgcgtcggca gtgtcttcat
     3541 gataaccgat ggtgaggtcg atgacattcc agccgatcca ggagcacttg gtttcaaagc
     3601 tccacttcat gcccttatca ctggccatga tggggaacgt gaccggcgaa tcgaactttt
     3661 ggaggcgccc cgcttcggga ttgttggcaa agagcaaacg atcgagctaa aagtgaacga
     3721 cacggcggat accggcgatc ctgttatcct aaatgttcgc cgtgacggca atcccatcgc
     3781 gagaatggcc acggccccgg gagagcgcat tcgcttgccg gtcaagattg aacatggtgg
     3841 cccaacagtt atagagctcg aggtcgaggc attgccggac gaactcaccg caatcaacaa
     3901 taaggccgtg ctcacaatcg aaggcgtgcg cgataagttg aaggtcctat tggtgtcggg
     3961 cgagccgcat gcgggcgagc gcatgtggcg caatcttctt aagtcggacc ccaatgttga
     4021 tctcgttcat ttcacaattc tgcgcccgcc ggaaaagcag gatggcacgc cgatcaacga
     4081 attgtcactg atcgcatttc cgaccagcga tctcttcggc cggaagatca aggaatttga
     4141 tctcatcgtc ttcgaccgct attcgaatca gagcgtgctg ccgctaattt atttcgacaa
     4201 catcgtccgg tatgtgcgtg aaggcggcgc gctgcttctc gcagcgggcc ccgattttgc
     4261 ccgtccggaa ggcctttatt attctccctt ggggagaatc gcacccgcgc gccccgaggg
     4321 cggcctggcc gaacacgcct tccgggccgc aatcaccgaa gatggcgcca aacatccggt
     4381 gacgcgcgga ttgcccggcg ccgatcaggc gccgcctgcg tggagcgaat ggttccgtca
     4441 aatcaacgcc gaggtcacgc atggcacggc tataatgtcc ggagctgctg gaaaaccgct
     4501 tctcgttgtt tcgcgcgagg gaaagggccg cgtggcgttg cttttgaccg atcagatgtg
     4561 gctctgggcg cgcggctttg aaggcggggg cccgaccctc gatcttttgc gcagactcgc
     4621 acattggctg atgaaggagc cagatttgga ggaggaggcc ttgcgggcca atgtgcgcgg
     4681 gcgcagcatc gaaatcgaac gccagagctt gaagggcgat tctccccagg tcacggtcac
     4741 cgcgccaaaa ggtgctacgg aaagcatccc gcttaaggcc gcggaaccag gcatctctcg
     4801 cgcaaccgtc gatgcagcgg aaatgggtct ctatcgcata agcgacggaa gtctctccgt
     4861 gctcgccaat gtggggccgg aaaatccgcg cgaatttcaa aacgtggttt cgacgctgga
     4921 caagctaaag ccgatcgccg aatcgacggg gggcacggtg cgccggatcg ccaatgggaa
     4981 aaacggcata accgtaccgc gccttgttgc gattggggat agtcccgtct atggcggtgc
     5041 tggctatgcc gccatcaagc gctcgggcgc cagcgaattg aagggcgtga gcatggcccc
     5101 actcgcgatc ggctttttgg gtcttgccgc gttgttgggg agcgttctgt tggcttgggt
     5161 ttttgaggga cataacagga gctggtcgcg ccgcacacgg ggttgattgc cggaggctgc
     5221 tgtcattcag gcgtggttcg ctttcgacgg cgtcggctta ctttcttgcg aggcccgccg
     5281 cacttttccc gtcacccctt cgaaagcgcc ttcgacaagc tcgcaataaa gcagacgctg
     5341 tggatcgacg ggcccggcga aaatgagtct tttggagggg acaggcaaaa aacctatttt
     5401 gctgtaatag gacatgtcgc cggccagcag caccaattca tgcccggcca cgcgagccgc
     5461 gttaagcgat gtcttcacaa gctcttcgcc gattccgctg gaacgaaagg gcggatcgac
     5521 cgtcagagga ccaagtagca gcgctggcct gtcgccgcaa aaaataggcg tcatccggat
     5581 ggccccaaca agcaaagtgc caacccgcgc gacgaaggac aagccgtagt ccggctcgat
     5641 cccttcgcgc agacggtagg cggaacgcgc aaagcggccc ggcccaaaag cgcgttcatc
     5701 gagcttctcg attgccgcga gatcggctgg ggtttgcggg ctgagggtga gaggcagatc
     5761 cgccattggt tttactgaga ggcgcgcggg gtaacgctgc ctgccattca tggtgcaaag
     5821 caactatcaa tcaagggcat ataacgcaaa ccaatcagcc ctgtgatcag aaagtggctg
     5881 cgtttttgca aatcttgaat cgccagctct gggcaaggcc gctcagcgca atatcgggct
     5941 cggaatccgg cacgaatgaa attgcctcgc tgacatggcc caaacggagc acggcgcgat
     6001 caggcggccg ccattgaacg ttccaaccgg tacggacatt catcaggcgg ttgatcttct
     6061 tcgggcgggc aaaaaagaag agtgccgcgg ttgcgtgcgg gccttgtact ccgggggcga
     6121 gacaaaccac gtaacatgca atctcgcggg attggtctct ctctcccttg acgaatatct
     6181 gatggcgctc caatggttcg accgcgcctt ggccctttat cccgcatatc tcgacgcctt
     6241 ggcaaaccgg ggcgtggccc tgcaacaagc cggccgccac ccggaagcgc ttgccgacta
     6301 tgacgaagcc atacgttggg gatgctccaa acctgaactt ttctataact gcggcaacat
     6361 cctgcgcgaa tcgggacggc ttgccgaagc cattgcttcc tatgatatcg cgttgcgtct
     6421 cgagcctgcc tacccggaag ccttgcg
//
LOCUS       DATMPU010000187         3247 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_1393723,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000187 DATMPU010000000
VERSION     DATMPU010000187.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 3247)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3247)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3247
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1393723"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..214
                     /locus_tag="VNY06_04350"
     CDS             <1..214
                     /locus_tag="VNY06_04350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012590167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HWX14082.1"
                     /translation="GREQEIEADGILATCLQHEVDHLNGVLFIDHISRLKRELLMKKF
                     RKNAKQKAQSNKGGAAKERATANHGA"
     gene            259..1209
                     /gene="fmt"
                     /locus_tag="VNY06_04355"
     CDS             259..1209
                     /gene="fmt"
                     /locus_tag="VNY06_04355"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007009652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HWX14083.1"
                     /translation="MRIVFMGTPAFAVPALEALIAKGHRVPAVYTRAPKPAGRRGLEL
                     IPSPVHEAALHLGIKVVAPKSLRSADAVAVLESFAPDAVVVAAYGLILPKAILEVPKR
                     GCFNLHASLLPRWRGAAPVQRAIMAGDRETGVMVMRMEERLDEGPVALTEKIAIGEKA
                     NAGEIASKLAHLGAGLMVQALSALEQGELIFTPQAEDGASYAPKIDKNELQIDWSRPA
                     TEVHNHVRGLAPFPAAHFAANLGKKLERIKVLRTEISQAEGTPGLILDDRLTIACGEK
                     SVRLVEVQRPGKRPMKAGNFLRGARIGKGTALPLTKDAAV"
     gene            1196..1951
                     /gene="truA"
                     /locus_tag="VNY06_04360"
     CDS             1196..1951
                     /gene="truA"
                     /locus_tag="VNY06_04360"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012590169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HWX14084.1"
                     /translation="MPRFKLSIEYDGLPFAGWQRQKNGLSVQEAIETALEALSGERAL
                     VGGAGRTDAGVHALGQVAHVDLAKDWPLDVLRDGLNAHLRPHPVAILAAELVPKTFDA
                     RFSARMRHYLYRIVNRRAPLTVWRGRAWHVARKLDTSAMQQAAQHLVGRHDFSTFRSS
                     ECQAASPVRTLERLDVARAAEEIHITASARSFLHTQMRSIAGSLEHVGSGRWSVDDFV
                     AARDAHDRSRCGMIAPPDGLYLMGVDYELFGGR"
     gene            2282..>3247
                     /locus_tag="VNY06_04365"
     CDS             2282..>3247
                     /locus_tag="VNY06_04365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX14085.1"
                     /translation="MAAMAAIGKAATIPLSRFTFTASKDGRQYTDVIVGQNPFAATHT
                     PTHVKILLVPMVIHIGSQVFDSTAPNFCGGSMGHSDLQNFRGSPILTPVTFDGGIGPG
                     RAAKVNGVNMGTRTYNDTHRRAEFLKAIGGPASPYGTFYDVTIAPTQTISAATSAFHS
                     AILTGSGCSLLGGLDFNFFDHYFATTVLNAVHGDPSSFVIFLMRDTVFYPGNRFNCCI
                     LGYHGTLGNLQTYSPTDYDTSGDFGPGIVNVSIAAHEIGEWLDDPLGTNPTPPWGGIG
                     QVGGCQGNWEVGDPLTGTDFPAIKMPNKVTYNPQENAFWSWFYSKD"
BASE COUNT          675 a          937 c          960 g          675 t
ORIGIN      
        1 gggacgcgag caggagatcg aggcggatgg gattttggca acctgtctcc agcatgaggt
       61 cgatcatctg aatggtgttt tgttcatcga tcatatttcc cgcctaaagc gggaactgct
      121 catgaaaaag ttcaggaaga acgccaagca gaaggcgcaa agcaataagg gcggcgcagc
      181 caaggagcgc gcgaccgcga accacggagc ctagacggcg tcgcgcgcca aagtcgccat
      241 agcattagag caaaactctt gcgcatcgtt ttcatgggca ctccggcatt tgccgttcca
      301 gcgctcgaag cactcatcgc caaaggtcat cgcgtccccg cggtctacac gcgtgcacca
      361 aagccagcag ggcggcgcgg tctcgagctt attccttcgc ccgttcatga ggcggcgctc
      421 caccttggga tcaaggtggt cgcgccaaag tcgcttcgct ccgcggacgc cgtcgcggtg
      481 ctggaaagct tcgcgcccga tgctgtggtt gttgccgctt atggcttgat cctgccaaaa
      541 gccattctcg aagtgccaaa aagaggatgc ttcaatctcc atgcttcctt gctgccgcga
      601 tggcgcgggg cggcgccagt ccagcgcgcc atcatggcgg gtgatcggga aaccggcgtc
      661 atggtcatgc gcatggagga gcggctcgat gaaggcccgg tcgcgctcac ggagaaaatc
      721 gcgattggcg aaaaagcgaa tgccggcgaa atcgcaagta agctcgccca cctcggcgcc
      781 gggctcatgg tgcaggctct ttcggcgctg gagcagggcg aacttatttt taccccgcag
      841 gctgaggacg gcgcgagcta cgcgccaaaa atcgacaaaa atgagctgca gatcgactgg
      901 agtcgacccg caacagaagt gcacaatcat gtccgcggtc ttgcgccctt tcccgccgcc
      961 cattttgcgg ccaatttggg caagaagctg gagcgcataa aagtcttgcg caccgagatt
     1021 tcgcaagccg aggggacgcc aggcctcatc cttgatgatc gcttgacgat tgcctgcggc
     1081 gaaaaatcgg tccgccttgt tgaggtgcag cgcccaggca agcggccaat gaaggctgga
     1141 aattttttgc gtggcgcgcg cattggcaag ggcacggctc tccctttgac caaagatgcc
     1201 gcggtttaag cttagcatcg aatatgatgg tttgcccttt gccggctggc agcggcagaa
     1261 gaatgggctc tccgtgcagg aagcaatcga gaccgcgctc gaagccctga gtggcgaaag
     1321 agctttggtt ggcggcgccg gacgcacgga tgcgggagtc catgcgctgg ggcaggtcgc
     1381 ccatgtggat ctcgccaaag actggcccct cgacgtgctg cgcgatggtc tcaatgccca
     1441 tttgcgcccg cacccggtcg ctatcctggc ggccgaactt gtgcctaaaa cttttgatgc
     1501 gcgattctcg gctcgcatgc gccattatct ctaccggatc gtcaatcgcc gcgcgccctt
     1561 gacggtttgg cgcggccgcg cctggcacgt tgcgcgaaag ctcgatacct ccgcaatgca
     1621 acaggcggcg cagcatctcg tcgggcggca tgatttttcg acctttcgct cttccgaatg
     1681 ccaggcagca agccccgtgc gcacattgga gcggctcgat gttgcaaggg cagctgagga
     1741 aattcacatc acggcgagcg ccagatcgtt tctgcacact caaatgcgct cgatcgcggg
     1801 ctcgctcgaa catgtgggta gtggcagatg gagcgtcgat gattttgttg ccgcgcgcga
     1861 tgcgcacgac cggagccgtt gtggcatgat tgcgccgcct gatgggcttt atctcatggg
     1921 cgtggactac gagctttttg gggggcgatg attcgaagcg tgcggttttc gagcgtgtcc
     1981 agcaaaagtg aaaaagggtt ttgcgtccag acacgcgaca aactcgagat ttagtacaat
     2041 ttgtgcgaaa tcgcacacgc cgaaggccgc cggatgtata agaattgttc gttcctgggg
     2101 caaaattgta gtagtggaga gaattatgaa tcgtacactc ttgtcagttc tactattaat
     2161 attgtgcttt gcctcactcg ccctgacggc gagcaacggt aaggtgcatg cccaagccct
     2221 gccgaccccg ctgaggggct actccatagt tattccgtac ccggggagcc atgaggacgc
     2281 gatggccgca atggccgcga ttggcaaagc tgcaaccatc ccgctgagta gatttacctt
     2341 cacggccagc aaggatggtc gacaatacac cgatgtgatc gtggggcaga acccatttgc
     2401 ggcaactcac accccgacgc acgtcaaaat actcctggtt ccgatggtca ttcacattgg
     2461 cagccaggtg ttcgattcga ccgctccaaa tttctgcggc gggtcgatgg gacactctga
     2521 ccttcaaaac ttccggggct cgccgatcct tacccccgtg acttttgatg ggggcatagg
     2581 ccccggacgc gcggcaaaag tcaatggcgt taacatgggc acgcggacct acaatgacac
     2641 gcatcgccgg gccgaattct tgaaggcaat cggcggcccc gcgagccctt acggcacgtt
     2701 ctacgatgtc accattgcgc ccacacagac gataagcgcg gccacgtccg ccttccatag
     2761 cgcgatcctt actggcagcg gctgcagctt gttgggcggt ctcgatttta atttctttga
     2821 ccattatttc gccacgaccg ttctcaacgc ggttcatggc gacccgagct ccttcgtgat
     2881 cttcctgatg cgcgacacgg tgttctaccc gggcaacagg ttcaactgct gcatcctggg
     2941 gtatcacggc acactcggca atctgcagac ctactcccca accgattacg acacctccgg
     3001 agacttcggt cccgggatcg taaatgtttc gattgctgcg catgaaattg gcgaatggct
     3061 ggacgatccg ctcggtacta atccgacccc cccatgggga ggcatcgggc aagtaggggg
     3121 ttgtcagggc aactgggagg ttggcgaccc gttgaccggt accgattttc ctgcaatcaa
     3181 gatgcctaac aaggtcactt acaacccgca ggagaatgcg ttctggtcat ggttctattc
     3241 caaggac
//
LOCUS       DATMPU010000188         4944 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_1695208,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000188 DATMPU010000000
VERSION     DATMPU010000188.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 4944)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4944)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4944
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1695208"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(381..1658)
                     /gene="tolB"
                     /locus_tag="VNY06_04370"
     CDS             complement(381..1658)
                     /gene="tolB"
                     /locus_tag="VNY06_04370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020186384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system beta propeller repeat protein
                     TolB"
                     /protein_id="HWX14086.1"
                     /translation="MLSGLGAASAAFAAQDRYLDVRGAGEFKPVQIAITAFAGDGAAG
                     SQLTGIITNNFKRSVFLAPIDPTTFVETVTNPDQVPRMDSWRMINAQFVVTGRVNHAT
                     DGRLQTEFRLWDVATGGQVSGSQYVTDPNNSRRVAHIISDAIFSRITGEKGFFDTRIA
                     FVDETGPPENRRKRLAIMDQDGANMRYMTRGDDLVVTPRFSPSSQDVTYMSFGASDPK
                     VLLLNIETGQREVVGNFPGMTFSPRFSPDGQKVVMSLAQGVASNLYSMDLHSRATTRL
                     SDSNAIDTSPSYSPDGTQIVFESDRGGSQQLYVMSAGGGGPRRISFGEGRYATPVWSP
                     KGDYIAFTRQKGGTFAIGVMRPDGTGERILTEGFHNEGPTWAPNGLYLMFFRDPGGQE
                     GPKIYMTDVFGRAEFPVPTPNYASDPSWSPPLD"
     gene            complement(1748..2830)
                     /locus_tag="VNY06_04375"
     CDS             complement(1748..2830)
                     /locus_tag="VNY06_04375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012589960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope biogenesis protein TolA"
                     /protein_id="HWX14087.1"
                     /translation="MALPESPGFAVSGGVHIGLLALALVSFSQTPQLTDAQESIPVAV
                     VSNEQFSQIMKGEKTAAEAKPQQRSEKIAQTAQLSAKPSPADAPKDVMAPPSPIKRQP
                     EPGQAEPEEAAKPPQQSIALPPQRPSQDAPKPAEKSEPVKQSVVPQPKPRIAEDADEV
                     SPQSAQKKPAEAAKKPAPKFKPDQLAKLLEQEKQKEAQKPAAKPKSGEEAAEPQRKFD
                     LGDIAKFLNKEAPRRKQASGHQLSQLASLGAPTASADKMSPSLWNQLDGLLQEQYKRC
                     WSFPGLGGQQKYIPEIHVQYRADGGLIGQPELLNPPSDPSLQALAESALRAVRRCDPL
                     RIPAQYQPYYDQWKGRIVRFDPEEML"
     gene            complement(2837..3286)
                     /locus_tag="VNY06_04380"
     CDS             complement(2837..3286)
                     /locus_tag="VNY06_04380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012383285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HWX14088.1"
                     /translation="MGMSGMAIGQGRKRGRRGVPRYRAMAEINMTPFIDVILVLLIIF
                     MVAAPLLATGVAIDLPKTKAAALNINRKPISVAVDDKGEVFVMDQPVEVNLLADKLKE
                     VATAADDDRIYVRGANQVNYGRIAEVMSVITGAGYKKVALVTDPNKN"
     gene            complement(3361..4092)
                     /gene="tolQ"
                     /locus_tag="VNY06_04385"
     CDS             complement(3361..4092)
                     /gene="tolQ"
                     /locus_tag="VNY06_04385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012589962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein TolQ"
                     /protein_id="HWX14089.1"
                     /translation="MNPADIAASGLTPPSVEVSLLGMFWSAHLVVKFVMVGLLGASVW
                     CWAIIINKLLLFARTERAMAKFEEVFWSGSSLDELYATLSSRPASGMAALFVSAMREW
                     RRSVKSASSSFMGLQARIEKVLDVSIVREVEKLESNLLVLATVASAGPFIGLFGTVWG
                     IMTAFRSIAVSRNTSLAVVAPGIAEALFATAIGLFAAIPALIAYNKFQGDVAKKQARL
                     EGFADEFSAILSRQIDQRGVQDRAA"
     gene            complement(4315..4788)
                     /gene="ybgC"
                     /locus_tag="VNY06_04390"
     CDS             complement(4315..4788)
                     /gene="ybgC"
                     /locus_tag="VNY06_04390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018265583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HWX14090.1"
                     /translation="MMLPAGTSLGKPAGKDLRLSGAQRQLAVRVYYEDTDFSGNVYHA
                     SYLRFLERGRTELLRALGIEHSRIFDSGGAKGCYFVVRAMTLEFLKPALMDDVLFVET
                     RVSGTGGASIEMNQEIRRGQVVLLTAKVRIAVVSGGKARRIPVEVLARLKAVARP"
BASE COUNT         1059 a         1438 c         1378 g         1069 t
ORIGIN      
        1 aaggttaaca aataattaat ggcgagaatg cggctgagtt gaagtttgtc caccacaatt
       61 ggcagaagtt gttgcggcgc gacgttaaga aaactccgcc gggggatgga caagtttgag
      121 attaaactat tgtttttatc gctattatcg acccaccaaa aaaagtagcg ctatagctgt
      181 cacaatttga aggcttgacc acctggaggg gcatcgattt gcctgtccgg agcctgcggc
      241 gaatcagtag agcgttaggc tgaggcgtga tctaggcacg cgacgacgcc aatcgtaaca
      301 cttctacccg ctgccgcacg ctgcggcaca tcatttcggc accgagtgtc tcgaaaccgg
      361 cgtcttttgc cgaacacgct ctaatcgagg ggcgggctcc aggacggatc cgacgcgtaa
      421 ttcggcgtcg gcactgggaa ttcggcacgg ccaaacacgt ctgtcatgta aatctttggc
      481 ccttcctgcc cgcccggatc acggaaaaac atcaaataga gaccattcgg cgcccatgtc
      541 ggcccctcat tgtggaagcc ctcagtcagg atccgttcgc ccgttccatc cgggcgcatg
      601 acgccaatcg cgaaagtgcc gcccttttgc ctggtgaagg cgatatagtc gcccttcggc
      661 gaccagaccg gcgtcgcata gcgcccttcg ccaaaggaaa tgcgcctcgg cccgcctccg
      721 cctgccgaca tgacatagag ctgctggctg ccgccccggt ccgactcaaa gacgatctgg
      781 gtaccgtcgg gcgaatagga aggcgacgtg tctatcgcgt tggaatcgga aaggcgcgtc
      841 gtcgcgcgtg agtgcaaatc catgctgtag aggttcgacg ccacgccttg ggcaagcgac
      901 atgaccacct tttgaccgtc cggcgaaaaa cgcggggaga aggtcatgcc cgggaaattg
      961 ccgacgacct cacgctggcc cgtctcgatg ttcaaaagca atactttggg atcggacgcg
     1021 ccaaacgaca tataggtgac atcttgcgac gaaggcgaaa atctcggtgt caccacgaga
     1081 tcatcgccgc gcgtcatata gcgcatattg gcgccgtcct gatccatgat ggcgagccgc
     1141 tttcggcggt tctcgggcgg accggtttcg tccacaaacg caatccgcgt atcgaaaaag
     1201 cccttttctc cggtgatccg cgagaaaatc gcatccgaga tgatatgcgc gacgcggcgg
     1261 gaattgttcg gatcggtgac atattggctg ccgctgacct gcccgcccgt cgccacgtcc
     1321 caaagccgga attcggtctg caatcgccca tccgtggcat ggttgacgcg gccggtcacc
     1381 acaaattgcg cattgatcat gcgccaggaa tccatccgcg gaacttggtc tggattggtt
     1441 accgtttcga cgaatgttgt tggatcaatc ggcgcgagaa aaaccgagcg cttaaaattg
     1501 ttggtaatga tcccagtcag ctggcttccc gccgcgccat ctccggcaaa cgccgttatc
     1561 gcgatttgaa cgggtttgaa ttcgccagcc ccgcgcacat cgagataacg gtcctgcgcg
     1621 gcaaaggctg cggacgcagc tcctaagccg ctcaaaatga ccgctgctcg aaaaagcgtg
     1681 gcggcggttt cccctatcgt gttgcgattg cgacgcgcaa aggcaggaag cgcggagaag
     1741 aacaaaatca caacatttcc tcgggatcga aacgaacgat ccggcccttc cattggtcgt
     1801 aatagggctg gtattgcgcg ggtatccgaa gcgggtcgca tcgccgcacc gcccgcagcg
     1861 cgctctcggc aagagcctgc aaactcgggt ccgagggcgg gttgagcaac tcgggctgcc
     1921 cgatcaaacc gccgtccgct cgatattgca catgaatctc agggatatat ttttgctgcc
     1981 ctcctagtcc ggggaagctc cagcatcgct tatattgctc ctgcagtagc ccgtcgagct
     2041 ggttccagag agacggcgac atcttgtcgg cgcttgccgt cggcgcgccg agcgacgcca
     2101 gctgactgag ctgatgcccg cttgcctgtt ttctccgcgg cgcctccttg ttcaaaaact
     2161 tggcaatatc gccgagatca aatttgcgtt gcggctcggc ggcttcctct ccggattttg
     2221 gctttgctgc gggcttctgt gcctcttttt gcttctcctg ctcgagcaat ttggccaatt
     2281 gatccggctt gaatttcggc gcgggctttt ttgcagcttc cgctggcttt ttctgcgccg
     2341 attggggaga aacttcatcc gcgtcttccg cgatcctggg ttttggctgt ggcacaacgg
     2401 actgcttgac aggttcggat ttctccgcag gtttcggcgc gtcctgcgag gggcgctgcg
     2461 gtggcaaggc tatgctttgc tgaggaggtt ttgcagcctc ctcaggctcg gcttgccctg
     2521 gctctggctg gcgttttatc ggggaaggcg gagccatgac gtccttcggg gcatcggccg
     2581 gcgagggctt tgcgctcaac tgcgccgttt gtgcgatttt ttcgcttcgc tgctgcggct
     2641 ttgcctcggc cgcagtcttt tcccccttca tgatttggct gaattgctcg ttgctgacga
     2701 ctgcgacagg aatgctttcc tgcgcatcgg tcagttgagg ggtttgcgaa aacgagacca
     2761 gcgccaaggc caaaaggccg atatgcacgc cgcccgagac cgcgaagcca ggactttcgg
     2821 gcagagccat gggcgtttaa ttcttgttgg ggtcagtgac caatgcgacc tttttgtatc
     2881 cggccccggt aatgacggac atgacctcgg cgatccgccc gtaattcacc tgatttgctc
     2941 cgcgcacata aatccggtcg tcatcggccg ccgttgcgac ctctttcaat ttatcggcga
     3001 ggaggttcac ttccaccggc tggtccatga caaaaacctc gcctttgtca tcgacagcga
     3061 cggaaatcgg ctttcggtta atattgaggg cggctgcctt ggtcttcggc aaatcgatag
     3121 cgacgccggt tgcgagcagc ggggcggcca ccatgaagat gatcaaaaga accagtatga
     3181 cgtcgatgaa aggcgtcatg ttgatttcgg ccatcgcgcg atagcgcgga acgccgcgtc
     3241 gtccccgctt gcgcccttgc cctattgcca tgccggacat acccatttca attctctccg
     3301 ccgcgagtta tcatattatt ctatgataaa atacccccgt ttaggggtgc gatttcgcac
     3361 tcatgcggcg cggtcctgta cgccgcgctg atcgatctgc cgcgataaaa tcgccgaaaa
     3421 ttcgtctgcg aaaccctcga gcctcgcttg tttcttggcg acatcgcctt ggaacttgtt
     3481 ataggcgatc agcgccggga ttgccgcgaa gagcccgatt gcggtcgcga aaagcgcttc
     3541 cgctatgcca ggcgcgacaa ccgcgagcga cgtattgcgg gataccgcga tcgagcgaaa
     3601 ggctgtcatg atcccccaaa ccgtgccgaa gaggccgata aagggaccgg ccgaagcgac
     3661 ggtcgccagc accaagagat ttgattcgag cttttcgacc tcgcgcacga ttgaaacgtc
     3721 gaggactttt tcgatgcgcg cctgcaggcc catgaacgag gaagacgcac tttttaccga
     3781 gcgccgccat tcccgcatgg ccgagacgaa caacgccgcc atacccgagg ccgggcgtga
     3841 cgagagtgtt gcgtaaagtt cgtcaagcga agagcccgac caaaaaactt cctcgaattt
     3901 ggccatggcg cgctcagttc tcgcaaaaag cagcaatttg ttgataatga tcgcccaaca
     3961 ccaaaccgac gccccgagca gccccaccat cacgaacttg accaccaaat gggccgacca
     4021 aaacatgccg aggagggaca cttccaccga aggcggcgtc agtccggaag ctgcaatatc
     4081 ggcgggattc attaaattgg tccttccaaa agggtaaacg gggcgggcaa gccgaggcgg
     4141 tctgccccag ggtttaaatt ttgctaggga aatccgtcaa ttctggggct tcgcacgggc
     4201 aaaacttctc cctggcctac gccaaaaaac ctgatcttgg tccagcaaac gttaacttct
     4261 ttttaggtta atatttggtt atcatgatga ttttgcaaga aatcaccctc aaggctaagg
     4321 ccttgcgacc gccttcagcc ttgccaaaac ttccaccgga atgcgtcttg ccttgcctcc
     4381 ggagacaact gctatcctaa ccttcgccgt caaaagaact acctgcccgc gccgaatttc
     4441 ttgattcatc tcgattgacg cgccgcctgt gccggaaacg cgggtctcaa caaaaagaac
     4501 gtcatccata agcgcgggct ttaaaaactc tagggtcatt gcgcggacga cgaaatagca
     4561 tccttttgcc ccgccgctgt caaaaatcct gctgtgttct atgccgagcg cgcgcaaaag
     4621 ctccgtgcgg ccacgctcga gaaaccgcag gtaagaggca tgatacacat taccggaaaa
     4681 atccgtatct tcataataga cacgcacggc aagctgccgt tgcgctcctg agaggcgcag
     4741 atctttgcca gcgggcttgc ctaggctggt cccggctgga agcatcattt aagttcgcag
     4801 actccaaata acggccaaag acgcagccac aagccaatct gcggcggcgc aaaatggcgg
     4861 ataccacacg gggattgcga caaacttcca ggatttagac gcgagtttgg agccttggtg
     4921 gtgcgcatga acaaaccagg cact
//
LOCUS       DATMPU010000189         3555 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_4482801,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000189 DATMPU010000000
VERSION     DATMPU010000189.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 3555)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3555)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3555
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4482801"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..716)
                     /gene="eutC"
                     /locus_tag="VNY06_04395"
     CDS             complement(<1..716)
                     /gene="eutC"
                     /locus_tag="VNY06_04395"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020174101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008851 - ethanolamine ammonia-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit EutC"
                     /protein_id="HWX14091.1"
                     /translation="MSGQARDFWDYLRRATPARIALGRTGDGLPTQRVLEFELAHARA
                     RDAVNTNLDPMALMAELEDWRPILVRSEAADRVAFLQRPDLGRRLAPNCAHLLTPGRY
                     DATIVIADGLSANAVQAQAEPLCKILLASQHFVFAPPVVALQARVALGDEIAVRQGAA
                     LVAVLIGERPGLSSFDSVGAYITFDPKPGASKDAERNCVSNIRAEGLSLKEAGRRILA
                     IMALARRISRTGTGLKEDEAL"
     gene            complement(713..2098)
                     /locus_tag="VNY06_04400"
     CDS             complement(713..2098)
                     /locus_tag="VNY06_04400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020174102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit EutB"
                     /protein_id="HWX14092.1"
                     /translation="MPYRCNAGGTSHVFADLRTLLARASPDRAGDRLAGLAAASNEER
                     VAARMALADFPLTRFLDDPLVPYDADEVTRLIIDTHDTDAFAPIAAMTTGELRDFLLS
                     DEATRETLAALAPGITPEMAAGVSKLMRNQDLIAVAKKISVVTRFRTTIGLPGRLCTR
                     IQPNHPTDDLAGIGASILDGLMYGCGDACIGINPASDSAKQALALLERIEELRERFEI
                     PVQSCVLAHVTTTLQVMEQGGAVDLVFQSIAGSEAANRAFGVNLALLDEARSAVESLH
                     RSPPGANLMYFETGQGAAQSADAHHGVDQQTMEARAYAVGRRYAPLLVNTVVGFIGPE
                     YLYDGKEIIRAGLEDHFCGKLMGLPMGVDVCYTNHAEASQDDMDNLLTLLCVAGCNYI
                     MGVPGADDIMLGYQSTSYHDALYVRSVLGLRPAPEFEAWLKSFGIMDQQARVRPSISG
                     GAARLLTASLQ"
     gene            2324..2620
                     /locus_tag="VNY06_04405"
     CDS             2324..2620
                     /locus_tag="VNY06_04405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012386010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HWX14093.1"
                     /translation="MNAPMESPKKSPVSTRGFASMDPEKQRAIARKGGESVPHEKRSF
                     SQNPRLAAEAGRKGGQSVNPSKRSFSRNHALASEAGRKGGHASHGGPKKPVTEG"
     gene            complement(2766..>3555)
                     /locus_tag="VNY06_04410"
     CDS             complement(2766..>3555)
                     /locus_tag="VNY06_04410"
                     /inference="COORDINATES: protein motif:HMM:NF014968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AprI/Inh family metalloprotease inhibitor"
                     /protein_id="HWX14094.1"
                     /translation="HKRSGGAKMGSKPSWRVQAAMIVGFAPLGWCAPLAAEVQAPTGI
                     WELSIDGTAGKCRISLRQEPSASGLRQITIPLACLRTFPVLGPVESWNLSGGDHLAFQ
                     GRAGQAILDFAAAAGAYVASGPNGETYHLAALNGAAAENVPRHEQGTKPRTSIKMAEE
                     PTANVQAEAAGRYSVLREGGKDTGCMLTLDGKGKAQPKGKASLAPGCRDQGIVIFDPA
                     SWEKVNGRLVLTARKGHKAHFDAQSDGSWKKDAAEGKTLSLKKL"
BASE COUNT          774 a         1072 c         1040 g          669 t
ORIGIN      
        1 agagcctcgt cctctttcaa tcccgtccct gtacggctga tcctccgggc aagcgccata
       61 attgcgagga tacggcggcc cgcttccttc aaggaaagtc cctccgcgcg gatattcgac
      121 acgcaattgc gctcggcatc tttgctcgcg ccgggtttcg gatcgaaagt gatataggcg
      181 cccacactgt caaaggagga gaggcctggg cgctcgccga taagcacggc aacgagcgcc
      241 gcgccttgcc gcaccgcgat ctcatcgccc aaagcaacgc gcgcttgaag cgcaacgacc
      301 ggaggcgcaa aaacgaaatg ttgtgaagcc aacagaatct tgcatagcgg ctctgcctgt
      361 gcttgcacag catttgccga aagcccatca gcgataacga tggtagcgtc atacctgcca
      421 ggggtaagca aatgcgcgca gtttggagcg aggcgacggc caaggtccgg acgctggaga
      481 aaagcgacac ggtctgcagc ctcgcttcgc acgaggatcg ggcgccagtc ctcgagttcg
      541 gccatcaaag ccatgggatc gagattggta ttgactgcat cccgagcgcg cgcatgagcg
      601 agctcgaatt caagcacacg ttgggtcggc aggccatcgc cggtccggcc gagcgcgata
      661 cgggcgggtg ttgcccggcg cagataatcc caaaaatctc tggcctgccc gctcattgca
      721 gggacgccgt taggagccgc gccgcacctc ccgaaatgct cggccgaacg cgcgcctgct
      781 ggtccatgat gccgaaagat ttgagccaag cctcgaactc cggcgccggc cgcaagccta
      841 aaacagagcg cacataaagc gcgtcatggt atgaggttga ttgatagccg agcatgatgt
      901 cgtcggcgcc gggaacgccc atgatataat tgcagccggc gacacaaagg agagtcaaaa
      961 gattgtccat gtcgtcctgg ctggcctcgg cgtggttggt gtagcaaacg tcaaccccca
     1021 tcggcagtcc catcagcttg ccgcagaaat gatcctcgag cccggcacgg atgatctcct
     1081 tgccatcata aagatattcc gggccgataa agccgacgac cgtattgacg agcagcggcg
     1141 cgtagcggcg cccaacggca taagcacgcg cttccattgt ttgctgatcg acgccatgat
     1201 gcgcgtcagc cgattgcgcg gccccttggc cggtttcgaa atacatgaga ttggcgcccg
     1261 gcggactccg gtgcagcgac tcgaccgccg aacgggcttc gtccagtaaa gcaaggttta
     1321 ccccaaaagc gcggttggcc gcctcagatc cggctataga ttgaaaaaca agatcgaccg
     1381 ccccgccttg ctccatgacc tgcaaagttg tcgtgacgtg agcgagcaca caagattgga
     1441 ccgggatttc gaaccgctcc cgcagctcct cgatccgttc gagtagcgcc aaggcctgct
     1501 tggcactatc cgaagcagga ttgatcccaa tgcaggcatc gccgcagcca tacatgaggc
     1561 catcgaggat ggatgcccca atcccggcga gatcgtcggt tgggtgattg ggctgaatgc
     1621 gcgtgcaaag ccggcccggc aaaccgatcg tcgtgcgaaa gcgcgtgact accgaaatct
     1681 ttttcgcgac ggcgatcaag tcctggttgc gcatcagttt cgacaccccc gcagccattt
     1741 cgggcgtgat gcctggcgcc agggctgcga gcgtttctct cgtcgcttcg tcggagagca
     1801 gaaaatcgcg aagttccccg gtggtcatgg cagcgattgg cgcaaaagca tccgtgtcat
     1861 gcgtatcgat gatgagccgc gtcacctcgt ccgcatcata aggcaccagg ggatcatcga
     1921 ggaaccgggt aagcggaaag tcggcaagcg ccatacgcgc ggcaacgcgt tcttcattgc
     1981 ttgcagcggc aagtccggcg agccggtcac cggcgcgatc cggcgaggcg cgcgccagaa
     2041 gcgtccgcag atccgcgaag acatggcttg tgccgccggc attgcatcgg taaggcatga
     2101 gctttcctgt tcaccaggac gctatacttt ctattgtaga gcaagctctg gctgaccgga
     2161 aaaccccaga gccggcaggc ccccaggcag taattttctt gtcgctttat tgatcgggcg
     2221 gctcctgcta atttggtcac gcgaacttga acgcaaaagg gcaaccttta ccgctgccaa
     2281 acattatcta attaaatcta gaacctattt tgagagagga atgatgaacg caccaatgga
     2341 atctcccaag aagtcgccgg tttcaacgcg gggattcgcc tccatggatc ccgagaagca
     2401 aagggcgatc gcgcgcaagg gcggcgagag cgtgccgcat gaaaagcgca gtttctcgca
     2461 aaacccgcgg cttgctgccg aagcaggtcg gaaaggcggc cagagcgtga acccaagcaa
     2521 acgcagcttt tcgcgtaacc acgcgctcgc atccgaggcg ggccgcaaag gcgggcacgc
     2581 gtcgcatggc ggtccaaaaa aacccgtgac cgaaggttga aagcaacagt ccatggagcc
     2641 aaagcaccta gagaaacccg gaggcggcat aaagtcgctc ccgggttgtg tggaggttat
     2701 ggacgggttc acggtttcaa agcctaacgc aagcggcccg ctcgcggttg aagacaaaaa
     2761 ctccgctata gtttttttag gctgagggtc ttgccttcag cggcgtcttt tttccagctg
     2821 ccgtcggatt gcgcgtcgaa atgggccttg tggcccttgc gggcagtcaa aacgaggcgt
     2881 ccgtttacct tctcccagct ggcgggatca aaaatcacaa taccttgatc gcggcagcct
     2941 ggagcaagag aggcctttcc ctttggctgc gcctttcctt taccgtccag ggtcaacata
     3001 cagccggtat ccttaccgcc ttcccgcaag acggaatagc gtcccgccgc ttcggcttgc
     3061 acatttgccg tcggctcctc tgccatctta atggacgttc gaggcttagt tccttgctca
     3121 tgacgaggta cattttccgc tgccgcgcca ttcagcgcag ccagatggta agtctctccg
     3181 ttcggcccag aggctacata ggcgcctgcc gctgccgcaa aatcgagaat cgcttgaccc
     3241 gcgcggccct gaaatgcgag gtggtcgccg cccgaaagat tccatgactc gaccggccca
     3301 agcacgggaa atgtcctgag gcaggccaaa gggattgtga tctgacggag cccggacgcg
     3361 gaaggttcct gtcgaagcga aatccggcat ttcccagccg tgccgtcaat agacaattcc
     3421 catattccgg tgggagcttg gacttccgcc gccagcgggg cgcaccaccc tagcggggca
     3481 aatccgacaa tcatggccgc ctgaacgcgc caactaggct tggaccccat tttcgcgccc
     3541 cccgatcgct tgtgg
//
LOCUS       DATMPU010000190         2303 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_2628300,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000190 DATMPU010000000
VERSION     DATMPU010000190.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 2303)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2303)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2303
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2628300"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            3..533
                     /locus_tag="VNY06_04415"
     CDS             3..533
                     /locus_tag="VNY06_04415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012591392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF447 domain-containing protein"
                     /protein_id="HWX14095.1"
                     /translation="MDRSGRAQLAPIGLIEDGQGWIIAPFRPSKTGDNLAETPFAIAN
                     YTDDASIFAGLITGRHDWPLAAAKKIPVPRLASALAHAELGVTRVEEDAERPRFHCEV
                     LHCEQHAPFAGFNRARNAILECAILLTRMHILPREKIDWEIDYLAIAVEKTAGPRERE
                     AWSWLMQKREAYYAAN"
     gene            complement(518..1555)
                     /locus_tag="VNY06_04420"
     CDS             complement(518..1555)
                     /locus_tag="VNY06_04420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012591391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HWX14096.1"
                     /translation="MQNVVGWDVGGAHLKGARAENGIIARAVQIASPLWLGVDALQRA
                     FFEADTVLGKAPLNAVTMTGELCDAFATREEGVTGLAVMMMRLLSPRRVVFYAGRSGF
                     VGADEIAKHLLEIASANWHASAALISTHVRGALFVDAGSTTTDIIPIADGKAANLGYT
                     DGERLSHGELVYTGIARSFLMAGPKLVPFGGRWTPLMNEWFATMADVYRILGRLPEGA
                     DMMETADGREKTKAASRARLARMVGRDSAEADDATWDRLAQFFAEIQLRELADAAALV
                     LSRGLYPSEAPVVGAGAGRELIRELAVRLRRPYLSFDELIETAPDARSKACDCAPASA
                     VALLAAAQFAA"
     gene            1591..>2303
                     /locus_tag="VNY06_04425"
     CDS             1591..>2303
                     /locus_tag="VNY06_04425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018266694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HisA/HisF-related TIM barrel protein"
                     /protein_id="HWX14097.1"
                     /translation="MPEPMIEVIPVIDLQGGGVVRARKGLRQSYVPIVTPLSRSSLPL
                     DVVTGLLTIHPFQTLYIADLDRIEMRGSHERCLEELRTAFPALTFWVDAGIRDAAEAR
                     AWLARHGQAHLVLGSESLQSLSVLEDRALLRRTLLSLDYRGDELIGPSGIWDLTKLWP
                     TRVIVMSLARVGTNFRPDLDRLAKIKRHAPESMIYAAGGLRDASDLMRLREVGISGVL
                     VASALYDGRLTGAHLAKTRT"
BASE COUNT          484 a          697 c          663 g          459 t
ORIGIN      
        1 ccatcgatcg gtcaggcagg gcgcaacttg cccctattgg tctcatcgag gatggtcaag
       61 gatggatcat cgcgccattt cgcccctcga aaacgggcga caatcttgcg gaaactccct
      121 ttgccattgc taattacacc gatgacgcct ctatcttcgc tggcctcata accgggcggc
      181 atgactggcc gctcgcagct gcgaaaaaaa ttccggtccc tcgcctcgcc tcggcgcttg
      241 cccatgctga gcttggtgtc acccgggtcg aggaggatgc cgagcgtcca cgtttccatt
      301 gtgaggtgtt gcattgcgag cagcatgcgc ccttcgcggg attcaaccgc gctcgcaacg
      361 cgattctgga atgcgctatt ctcctgaccc gaatgcatat cctgcctcgc gagaaaatcg
      421 actgggagat cgattatctt gcgattgctg tcgaaaaaac ggccgggccc agagagcgcg
      481 aggcctggtc ctggctcatg caaaagcggg aagcctatta cgccgcaaac tgagcggcgg
      541 cgagaagggc aacagccgag gcaggggcgc aatcgcaggc cttgctccgg gcgtccggag
      601 cagtctcaat gagttcgtcg aaactaagat aggggcggcg caagcgcacc gccagctcgc
      661 ggattagctc gcgaccagct cccgcgccga ccacgggcgc ctccgaggga taaagcccgc
      721 gcgacaaaac gagggccgcg gcgtcagcga gctcacgcaa ttgtatctcg gcgaaaaatt
      781 gcgcgagccg gtcccaagtg gcatcgtccg cctcggcact gtcgcgacca accatccgcg
      841 cgagacgcgc gcgggaagcg gcttttgtct tttcgcgccc atcggccgtc tccatcatat
      901 cggctccttc cggcaatcgt ccgagaatac gataaacgtc agccattgtg gcaaaccatt
      961 cattcatcaa tggcgtccat ctgccgccaa aagggacaag tttgggaccg gccatcaaga
     1021 aactgcgcgc gatgcccgta tagacaagct ccccatggga aagccgctcc ccgtctgtat
     1081 agccaaggtt tgctgcctta ccgtcggcga tagggatgat atcggtggtt gtcgaaccgg
     1141 catcgacgaa tagggcgccg cggacatgcg tagaaataag cgccgcgctc gcatgccaat
     1201 ttgccgaggc aatctcgagg agatgctttg cgatttcgtc agcccccaca aaacctgagc
     1261 ggccagcgta aaaaacgacg cgcctggggc taaggaggcg catcatcatc acagcaagtc
     1321 ctgtgacgcc ctcctcgcgg gtcgcaaagg catcgcacaa ttcgccagtc attgtaaccg
     1381 cgttgagcgg agccttcccg agcacagtat cggcttcaaa gaaagcgcgt tgaagcgcgt
     1441 cgacgccaag ccaaagcggg cttgcgattt gcactgctcg cgcaataatg ccgttttcgg
     1501 cacgcgcacc cttaagatgc gcgccaccga catcccatcc tacgacattt tgcattatct
     1561 agccaaatcc aggcggaaga tgggaagatt atgcccgagc cgatgattga agtcattcct
     1621 gtgatcgact tgcagggcgg cggcgtcgtg cgcgcgcgaa aagggttgcg ccagtcctat
     1681 gtgccgatcg taacgccgct ttcccgctcg agcctgccgc ttgatgtggt cacagggctt
     1741 ttgaccattc atccgtttca aacactttat atcgccgacc tcgaccggat agaaatgcgg
     1801 ggtagccatg agcggtgtct cgaagaattg cgcacagcat ttcctgccct taccttttgg
     1861 gtcgacgcgg ggatccgcga cgctgccgag gcgcgtgcat ggcttgctcg ccacggacag
     1921 gcgcatctcg ttcttggaag cgaatctttg cagagccttt ctgtgctcga agatcgcgcc
     1981 ttgctccgcc gaacactgtt gtcgctcgat tatcgcggcg atgaacttat cgggccgagc
     2041 ggaatttggg atctgacaaa gctttggccg acgcgcgtca tcgtcatgtc tttggcgcgc
     2101 gtcggtacaa atttccggcc tgacctcgat cgtctcgcaa agatcaaacg ccacgcgcca
     2161 gaaagcatga tttatgccgc aggggggctt cgggatgctt ccgacctcat gcggctgagg
     2221 gaagtaggca taagcggggt gcttgttgca tcagcactct acgacggccg cctgaccggc
     2281 gcacaccttg ccaaaactag aac
//
LOCUS       DATMPU010000191         2515 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_1090498,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000191 DATMPU010000000
VERSION     DATMPU010000191.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 2515)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2515)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2515
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1090498"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..302)
                     /locus_tag="VNY06_04430"
     CDS             complement(<1..302)
                     /locus_tag="VNY06_04430"
                     /inference="COORDINATES: protein motif:HMM:NF024750.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Arm DNA-binding domain-containing protein"
                     /protein_id="HWX14098.1"
                     /translation="MPRQATKLSAAKVRTAGPGKYCDGEVSGLYLMVRSARAKYWLFR
                     YTLRGKKMREMGLGAAVGPRMVSLADAREAARKARSLVAAGIDPLDERAAEKCAAS"
     gene            complement(404..493)
                     /locus_tag="VNY06_04435"
     tRNA            complement(404..493)
                     /locus_tag="VNY06_04435"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(457..459),aa:Ser,seq:tga)
     gene            750..1571
                     /locus_tag="VNY06_04440"
     CDS             750..1571
                     /locus_tag="VNY06_04440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012384594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX14099.1"
                     /translation="MKTLRLVLLGLAVLAGLFAGAALLKKAVRPEPASTSLTATASSR
                     PGEPVHNIETVRRTFENMLKGAPEYAVFFERLKADFPSEYESFLTAVAQRSAASGEIG
                     SADLHLAEAVRSLRLSRGVLAAKAGQAALEHIFELQLAMLKALAARDPRLCADYLYGG
                     ESSGYLEFAAQNRSLVAEMAVAGVDAIHDGELKRVERDAPTVGEFDALEQMLRAKGLG
                     SQEIEALLDGTVSNPPISDERMCNAGQVYLETLATMPEESRLRIYGLAVELMARS"
     gene            complement(1583..>2515)
                     /locus_tag="VNY06_04445"
     CDS             complement(1583..>2515)
                     /locus_tag="VNY06_04445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011085102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWX14100.1"
                     /translation="LPPEGWSPDGWTALFRQKAMISSNHVALKDAHKTLQFWLIWAVL
                     CLNVSASIGIIGAASAMLQETFGGALFHDPSVGFSQFSDAQKTLAAAIGGGFVGLFSL
                     FNIGGRFFWATLSDSIGRKTTYFVFFLLGMLCYSAASTLAGMKAVGIFAAAFCVIASM
                     YGGGFATVPAYLADIFGARYVGAIHGRLLTAWSTAGVIGPVIVNYLHDTQMAEGVSRD
                     QIYGVIFYVLAALLAAGFVCNTLIRPVKAKWHMAEEKGKKPAGHARVFGAISACEIGR
                     GSLDAKVVLAWTAVGIPLSWGVWKTLENAAKIFL"
BASE COUNT          537 a          750 c          706 g          522 t
ORIGIN      
        1 ttgctcgcgg cacatttttc ggcggcgcgc tcgtcgagcg ggtctatccc agcggcaact
       61 agggaacgag cctttcgtgc cgcctcgcgt gcatccgcca ggctgaccat tcttgggcca
      121 acggccgcac cgagccccat ctcgcgcatt tttttgccgc gtaaggtgta tcggaacagc
      181 caatattttg ctcgggcgga ccgcaccata agatagagcc cgctgacctc gccgtcgcaa
      241 tacttgcccg gccccgcagt gcgaaccttc gctgcgctga gtttcgtcgc ttgccgtggc
      301 atttcggata tcccctaatc tgtcccccat agccttaacg ctgcggtttc aatggcttgc
      361 tttgaattta tgggacggcg tcgaacgctg gcgaaaggct agatggcgga aggagtggga
      421 ttcgaaccca cggcgggctt gcacccacgg cggttttcaa gaccgctgcc ttaaaccact
      481 cggccatcct tccgcggaaa cagatggtgt ggccagtatc ttcggcgccg agaggcgaca
      541 gtcttattgc tccgatcaac cgggcagtcc agcctatttg ccgagtcccg gaaggggttc
      601 gatacaggaa tttgcaaaaa gagatacctg cctcaatggg ctcacgcggg cgcggggatg
      661 gtgaacggct ggtttgactt gcccgctccc cttcttttgg gcaatgtcag actcctcgtt
      721 cccttgttcc cgaagggagt ttcgccaaag tgaaaacctt gcggctcgtg cttttgggcc
      781 ttgccgtcct tgccgggctc ttcgccgggg cggcgctact gaaaaaggcg gttcgaccgg
      841 aaccagcatc cacctccttg accgcaacgg cgtcctcgcg cccgggtgag ccggttcata
      901 acatcgaaac cgtgcgccgc acgttcgaga acatgctcaa aggggctccg gaatatgcgg
      961 ttttttttga gcgcctgaaa gctgattttc cttccgagta tgagtccttt ctgacggctg
     1021 ttgcccagcg ttccgccgct agcggcgaga ttggaagcgc tgacttgcat ctggcggaag
     1081 ccgtccgttc gttacgactt tcacgtggcg tcctggcggc caaggccggg caggcggctc
     1141 tggagcatat ttttgaattg cagctcgcaa tgttgaaggc gctcgccgcc agggatcccc
     1201 gcctttgcgc cgattatctc tacggcggcg aaagttcggg ctatctcgaa tttgccgcgc
     1261 agaaccgttc acttgtggcg gagatggcag tcgccggcgt cgatgccatc catgacggcg
     1321 aattgaagcg cgtcgaaagg gacgcgccaa ccgtggggga attcgatgct cttgaacaaa
     1381 tgttgcgcgc caaagggctt ggtagccaag aaattgaagc tcttctcgac ggcacagtga
     1441 gcaatcctcc cattagcgac gaaagaatgt gcaacgcggg tcaggtttat ctcgaaaccc
     1501 ttgccacaat gccagaggag agccggttgc gcatctacgg cctagctgtc gaactcatgg
     1561 cgcggtcctg atatccggtc catcatagga atattttcgc cgcgttctcg agcgtcttcc
     1621 agacgcccca ggaaagcgga atcccgaccg ccgtccaggc cagaacaacc ttagcatcga
     1681 gactaccccg gccaatttca caggcgctga tcgctccaaa taccctggcg tggcccgctg
     1741 gtttcttgcc cttttcttcc gccatatgcc acttcgcttt gactggccgg atcagcgtat
     1801 tgcaaacgaa tccggcggcg agaagggcag cgagaacata gaaaatcacg ccatagattt
     1861 gatcgcgcga tacaccctcg gccatttgcg tgtcatggag ataattgaca attaccggac
     1921 cgataactcc ggctgtcgac caagcggtaa gcagccgccc atgaattgcg ccaacgtagc
     1981 gggcgccaaa aatatccgcg agataggcgg gcaccgtggc aaagccgcca ccatacatcg
     2041 aggcgattac gcaaaaggcc gccgcgaaaa tgccgaccgc tttcatgcct gcgagggtcg
     2101 atgcggcgct atagcaaagc atgccaagca ggaagaagac gaaataggtc gtcttccggc
     2161 cgattgaatc ggacagcgtt gcccagaaaa acctgccgcc aatattgaac aaagagaaaa
     2221 gtccgacaaa gccgccgccg attgcagccg ccaaagtctt ctgcgcgtcg gaaaactggc
     2281 tgaaaccaac cgaagggtca tggaacaatg cgccgccgaa ggtctcctgc agcatcgcag
     2341 atgccgcgcc gatgatcccg atactcgccg aaacattgag acacaggacc gcccatatca
     2401 accagaattg cagcgtctta tgcgcatcct tcaacgcgac atggttcgat gaaatcattg
     2461 ccttttgtct gaaaagagcc gtccagccgt ccggactcca tccctctggt ggcag
//
LOCUS       DATMPU010000192         2906 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_149664,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000192 DATMPU010000000
VERSION     DATMPU010000192.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 2906)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2906)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2906
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_149664"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..186)
                     /locus_tag="VNY06_04450"
     CDS             complement(<1..186)
                     /locus_tag="VNY06_04450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX14101.1"
                     /translation="MSELLSELHKLADRLSAPGARAFLRPFDALLIPQFHAAYFRSNE
                     GGCAIAGLITMFTQHTNS"
     gene            325..1701
                     /locus_tag="VNY06_04455"
     CDS             325..1701
                     /locus_tag="VNY06_04455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012589371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ActS/PrrB/RegB family redox-sensitive histidine
                     kinase"
                     /protein_id="HWX14102.1"
                     /translation="MADLINIDLGARSRRLRVDTLVKLRWLALLGQTIALLVTYFGLQ
                     FSLPLGACFLVIAASACLNTLLRLSFGRTGRLEDVPAAFLLAYDILQLTVLLYLTGGI
                     QNPFSVLYLAPIMISAVSLSGRITFLLTLLMIAAASALTFQHFPLPWFPGEQLQLPLI
                     YDAGIWIAIVLGAGFVAIYASRVAIEARKLADALAATELVIAREQHLAQLDGLAAAAA
                     HELGTPLATITLVVKEMQRQPSAGHPLEEDLALLAQEAGRCRAILGKLASLGGDSEDI
                     LDEMSLGVLIEEVTIPQRDFGVQISVADDGHGQEPVCRRNPGILYGLGNLIENAIDFA
                     ASEVQVAAKWNDAWVVIVISDDGPGFSPNVADRLGEPYVTTRTDRRAKTEEGSGLGLG
                     LFIAKTLLERAGATVKTENRAPPVRGAIVTITWPRDMFEQGTKRLSIPGKGASATVEP
                     GPRMTPIT"
     gene            1809..2411
                     /locus_tag="VNY06_04460"
     CDS             1809..2411
                     /locus_tag="VNY06_04460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003611013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ActR/PrrA/RegA family redox response regulator
                     transcription factor"
                     /protein_id="HWX14103.1"
                     /translation="MTDNPQRLVGTSVPGEKDDAQQLGAWSDRTLLIVDDDHTFLTRL
                     CRSMETRGFTVTGVGSVAEGLAIISEHAPAFAVIDMRLADGNGLDVIRELKAKRPDAR
                     GIILTGYGNIATAVTAVKLGAFDYLAKPADADEIFNALMAERHDKAELPENPMSADRV
                     RWEHIQRIYELCGRNVSETARRLSMHRRTLQRILGKKAPR"
     gene            2612..2684
                     /locus_tag="VNY06_04465"
     tRNA            2612..2684
                     /locus_tag="VNY06_04465"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2645..2647,aa:Ile,seq:cat)
BASE COUNT          592 a          819 c          838 g          657 t
ORIGIN      
        1 cgagttcgta tgctgcgtaa acatggtgat caggcctgcg atggcgcatc ccccttcgtt
       61 agaccggaaa taggctgcat ggaactgcgg aatcaataag gcatcaaatg gacgcagaaa
      121 agcgcgcgca cccggagcgg aaaggcgatc agctaactta tgcaattcgc ttaacaattc
      181 gctcaatatg tggtgatggc tcaaaaagtc cgcaacccca aacgagggac caaattcacc
      241 ttcgcactcg cacacaaggg cgatagcggc ctcctggcca gatgttagca tggctcctcg
      301 attgccgtca gcgaaagggc tggcatggcc gaccttatca acatcgacct tggggcccgc
      361 tcgcgccgcc tgcgcgtcga tacgctcgtc aaactccgct ggcttgccct gcttgggcag
      421 acgatcgcgc ttctggtcac ctattttggg ctccagtttt cgctaccgct tggcgcttgt
      481 tttctcgtca tagctgcctc cgcctgtctc aatactttgc tgcgattgag ctttggccgc
      541 accggcagac tcgaggatgt tccggccgca tttttgcttg cctatgacat tcttcaattg
      601 accgtcctcc tttatctaac cggcggaatt caaaatccgt tttcggttct ctatttggcg
      661 ccgatcatga tctccgccgt gtcgctgtcc ggccggatca cattcctgct gacattgctc
      721 atgatcgccg cggcatcggc cctcaccttc cagcattttc cactgccttg gtttccgggg
      781 gagcagttgc agctccccct gatctatgac gccggaattt ggatcgcgat tgtgcttggg
      841 gcaggatttg ttgcgattta tgcttcgcgg gtcgccatcg aggcccgcaa actcgccgac
      901 gcgttagcgg cgacagaact cgtgattgcc cgcgagcagc atctcgccca gcttgatggg
      961 ctggctgcgg ccgcggcgca tgaactcggc acgcccctcg ccacgatcac gctcgttgtc
     1021 aaggagatgc agcggcagcc ctccgccggc caccctttgg aggaggacct agccctgctc
     1081 gcacaagagg ctggtcgctg ccgtgcgatc ctcggcaagc tcgcttcgct gggcggagat
     1141 agcgaagaca tattggatga aatgagcctt ggggtgctta tcgaagaggt tacaattccg
     1201 caacgtgatt tcggcgtgca gatttccgtt gccgacgatg gtcatgggca agagcctgtc
     1261 tgccggcgta atccggggat attgtatggg ctcggcaatt tgatcgagaa tgcgatcgat
     1321 tttgccgctt cggaggtcca ggtcgcggcg aaatggaatg acgcttgggt cgtgatcgta
     1381 atctcggacg atggcccagg tttttcgccc aatgtggcag accggctggg tgaaccctac
     1441 gtcacaacaa gaaccgacag gcgcgccaag accgaggagg gatccgggct tggtcttggc
     1501 ctgttcattg ccaaaacgct tctcgagcga gcaggcgcaa cagtcaaaac tgaaaatcgt
     1561 gcgccgccgg tgaggggcgc gatcgtcacg atcacatggc caagggatat gttcgagcag
     1621 ggcaccaaac gcctatcaat tcccggcaaa ggcgcctctg ccacggtcga accggggccg
     1681 agaatgacgc cgattacctg aagcgaaatc ggggcgcttt ccgtctcaca aaagctatga
     1741 aaaaactccg ctccgctctt gtcttggcgt atttttttcc tttcaatggt ttaacaaggc
     1801 gaaaggcgat gacagataat ccgcaaaggc ttgttggaac ctcagttccc ggcgaaaagg
     1861 acgacgcaca acaattgggc gcttggtctg accgcacgct attgatcgtc gatgatgacc
     1921 ataccttttt gacccgcctt tgccgaagca tggagacgcg cggtttcacc gtgacgggcg
     1981 tgggctccgt ggcagaaggc ctcgctataa tctccgagca cgcccccgct ttcgcggtca
     2041 tcgacatgcg gctcgccgac ggtaatgggc tcgatgtgat ccgtgaatta aaggccaagc
     2101 ggccggatgc gcgcggcatt attctgaccg gctatggcaa tattgccact gccgtgaccg
     2161 ccgtgaaact tggcgccttt gattacttgg caaagccggc cgatgccgac gagattttta
     2221 atgctctgat ggccgagcgc catgataagg ctgagctccc ggaaaatcca atgtccgcag
     2281 accgggtacg gtgggagcac attcaacgga tttatgaact ttgcggccgc aatgtctctg
     2341 agacggcgcg aaggctcagc atgcaccggc gcaccttgca acgcattttg gggaaaaagg
     2401 cgccgcgtta ggcagcggta ggcggtgccc aggcgttgcg gccgacattg cttcgcggcc
     2461 caaattgcgt ttaatgcgtt tcccggcgtt tcaggcagcg ccttgctttg ccattcgctt
     2521 tgggcgggat ggttttgagg aggcggatta ggaatactga atcaccgcta tttgtaatat
     2581 tgggcggggc agtattgttc gaggtctgcc ggggccggta gctcaacggt tagagccggc
     2641 cgctcataac ggtctggttg caggttcgag tcctgcccgg cccactccta ttggaatgta
     2701 cattacaatt tcagttctat tccgctttcg aagaaaacta actgagcacc cattgggtac
     2761 ggggtcccca tattccgcca ttcagcgtga ccctcgaaat ggaccgagat tggccaggaa
     2821 cagacatcgt tgatgaagga tgctttcagc cgggccacac ttagcaggcg gccctaagcc
     2881 gacgcgtgat atgatacgat ttccgg
//
LOCUS       DATMPU010000193         3355 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_4175449,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000193 DATMPU010000000
VERSION     DATMPU010000193.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 3355)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3355)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3355
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4175449"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(434..754)
                     /locus_tag="VNY06_04470"
     CDS             complement(434..754)
                     /locus_tag="VNY06_04470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX14104.1"
                     /translation="MADGPVMRPDRHSLMAFGGIAALATGAAMLLADGGAVFAPVTGM
                     VGGIAAMALANHERLKLRIEVLDKRLAQAEWRGHPLRLNSGSGNLRFRYLCRRAGFLR
                     DRRT"
     gene            complement(1349..2971)
                     /locus_tag="VNY06_04475"
     CDS             complement(1349..2971)
                     /locus_tag="VNY06_04475"
                     /inference="COORDINATES: protein motif:HMM:NF041025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019239 - deaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-phage dCTP deaminase"
                     /protein_id="HWX14105.1"
                     /translation="MTDELQTGDPPSDAQGPEIFIGLIGAVGTDLYRIREQLKQELRR
                     ANYTPDIIHLSKLMIDLDNLESLAACDKGPEDDRIDKFMDAGDAIRDSISRGDAVVLL
                     GISKIQDIREENQPASARNKREGEAADGQRSPEIRPLYRRAYIFHSLKHPEEVRTLRR
                     VYGAAFIAISVYSPRNVRKQALCERIARSRKEYNSEKFAARADELIKKDEQEVGDDFG
                     QNVRDTFPEGDVFVDTTDPESIPSQIARLVNILFGYPYVTPTVDEYGMFHAKAAAMRS
                     ADLSRQVGAVITTADGEIITAGCNEVPKAGGGSVWEGRDADKGKDYRDHTLGYDSTAR
                     MKHETIAEIFERLLPDWLAVELRDLSPQELATKALYEGASPRLKGTRADSILEFGRMV
                     HAEMSAITDAARRGLSVLDATLYCTTFPCHMCARHIIASGIKHVVYIEPYPKSLAKEL
                     YRKSIQVDFDVEADSDAVVFRPFVGISPNRYLDFFSMPKRKDDQGHTLDWSSETAQPR
                     VERIGTYEEAEAGLMDLLAESRPRLGIKRAEQ"
     gene            3221..>3355
                     /locus_tag="VNY06_04480"
     CDS             3221..>3355
                     /locus_tag="VNY06_04480"
                     /inference="COORDINATES: protein motif:HMM:NF028194.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HWX14106.1"
                     /translation="MTASLARQIVLAARPNGKPQLSDFRLEETEVPAPASGQLLLAVQ
                     Y"
BASE COUNT          676 a         1017 c          899 g          763 t
ORIGIN      
        1 ggtcgaaata ttcgccgcgc cggtcgtagc gcttggcaag gccgaccagc tcacgccgga
       61 tgccgggcga ctttgtgtag cgcgccagct ggcgcagctt gccggccatc tcgcggcaat
      121 gatcgccgtc accaagcgcg ggcgcaggtt cgtcgattgc caatgagctc aagttgccgg
      181 tagtcaaaaa actcgcgggc ggggccaaca accggttaca ccaatccccg cccgcgtcgt
      241 cactatcagg gtcgtaagtc acacccggcc ggccaatgtg cgttattgcc gaatgccgcc
      301 gacaattacc tcggatacgc ctaaaggccc agatgtgaac atgcttaggg gcggcgcaat
      361 cgtcgttaaa tttgcgcgaa gggctccgta gcggcgcgga attcccggat tccggtgacc
      421 tatgggatga tcattatgtt cgccggtccc tgaggaaccc ggcacggcgg caaagataac
      481 gaaaccggag gttccccgaa cctgaattta accggagcgg gtggccccgc cactccgcct
      541 gcgccaggcg cttatccagc acctctatcc tcagcttgag ccgctcatga ttggccagcg
      601 ccatggcggc gatgccgccg accatgccgg tcaccggcgc gaacaccgcg ccgccatccg
      661 cgagcagcat cgcggcgccc gtcgcgagcg cggcgatccc gccgaacgcc atcaagctgt
      721 gccgatccgg ccgcatcacc ggaccatcgg ccatgcgcct taaaaattga tgatcattgt
      781 ctgcatcaac ctgtgggcgc gcctgtcact cgcaagcaag tgacaggcgc gctctaagcg
      841 ctgcgcgcgc ggagccgtcc ctttttcccc ctcgaggggg gcgtctccgc cctgtcgcgg
      901 aacgatcgat tctgtaatcg aatcgccgtc aagccccctc tatttcggca acgcctcacg
      961 ttgaatttcg gcttccgggg tcgggaggtc aattcaacgc gcggtcggcc gccagcgatc
     1021 agaacaggtt acaaatcacg ccgagcgcag gaccgctttc cacgttgaat gggccaagtt
     1081 tcactcggat ggaggggtag atcagccttc gtactccgat ttgataagtc ataaccggcg
     1141 agccgctacc gacttcacat tgtcaccgcc gctatctctt ctgcccgttt gatggctcgt
     1201 gctgcgtgat ccgctgctcc tggacgctcc gatatcagcg atctgggatc tgatcgtctc
     1261 tcgtttccac tcgctccaag accgaaccac gctgcttgcg tgatccaatt gccggttaag
     1321 ccaattgcgc ttcgccattt gcttccccct attgctccgc tcgcttgatg cctagtctcg
     1381 gtctgctttc tgccaaaaga tccatgagac cggcttcggc ttcctcatac gtaccaattc
     1441 gttctacgcg cggctgtgct gtttccgagc tccaatccaa tgtgtgaccc tgatcgtctt
     1501 tacgttttgg cattgagaag aaatcaagat atctgtttgg tgaaatccca acgaacggac
     1561 ggaaaacaac agcgtcacta tccgcctcga cgtcgaagtc tacttgtatc gatttgcggt
     1621 agagttcctt cgccaggctt tttgggtatg gctcgatata gaccacgtgt ttgatccctg
     1681 aggctatgat gtggcgcgca cacatatggc aagggaaggt ggtgcaatag agagtggcat
     1741 cgagaaccga gagaccccgt ctggctgcgt cagttatggc ggacatctcc gcatgcacca
     1801 tccgcccgaa ttcgaggatg ctgtccgcac gggttccctt cagccgaggg gaggctcctt
     1861 catacagagc tttcgtcgcc aactcctgag gcgataagtc ccgtaattca acagccaacc
     1921 aatcaggcag caagcgctcg aatatttctg caatcgtttc gtgcttcatt cgagctgttg
     1981 aatcatagcc cagggtgtga tcccgatagt cttttccttt gtccgcgtcg cgcccttccc
     2041 agacagaccc gccgcctgcc ttgggtactt cattacatcc agcggtgatg atttcgccgt
     2101 cggcggttgt gatcaccgct cccacttggc gggagagatc cgcagatcgc atcgctgccg
     2161 ccttggcatg gaacatcccg tactcatcta cggtcggtgt tacgtaggga tagccgaata
     2221 ggatattcac caaacgcgca atctgcgagg gaatgctctc gggatcggtt gtatcgacga
     2281 agacatcgcc ttccggaaag gtatcgcgga cgttttggcc aaagtcgtcg cccacttctt
     2341 gttcgtcttt tttaattaat tcatcagcgc gggcagcaaa tttctcgctg ttatattcct
     2401 tccgcgatct tgcaattcgc tcacaaagcg cctgttttcg cacgttcctc ggtgaataga
     2461 ctgaaatagc tataaaggca gcgccgtaaa cgcgccttaa agtgcgaact tcttccggat
     2521 gtttgagaga atggaagatg taggcccggc ggtaaagcgg gcgtatttct ggcgagcgtt
     2581 gcccatcagc cgcctctcct tctcgcttat tccgcgcaga ggctggttgg ttttcttcgc
     2641 ggatatcttg gattttgctg atcccaagaa gaacgacagc atcacctcgg gatatggagt
     2701 cacggattgc atccccggca tccatgaatt tatcaatgcg gtcgtcttct gggcctttgt
     2761 cgcaggcggc aagagattcc agattgtcca agtctatcat caatttgctt agatggatta
     2821 tgtccggggt gtagttggca cgcctcaatt cttgtttcag ttgctcgcga atgcgataca
     2881 aatccgttcc gaccgcgccg atcagtccga tgaaaatctc cggtccctgc gcatcggacg
     2941 gcggatcgcc ggtttgcagt tcatccgtca caaggtcgcc ccgccccctt ggccccaaag
     3001 ccggcatcca tttgattgta gcatcgatgc caaggcaaac ccgcccagta gacccttgat
     3061 atgtcgactc gcctggcaaa ggctcctacg gatacgtgca agcactgtgg gaatcggcgc
     3121 gccgaggcag atggccgagc aagcatgcgc ccttatggaa caaaaaaaga acttatgcaa
     3181 gtccacatgc acccttcgga tcgctgccga gaccataccg atgacagctt ctcttgcccg
     3241 ccaaatcgtc cttgccgctc ggccaaacgg caaaccgcaa ttgtccgact tccggcttga
     3301 ggaaaccgag gtcccagcgc cggcttccgg gcagcttctg ctcgcagtgc agtac
//
LOCUS       DATMPU010000194         8049 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_1397286,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000194 DATMPU010000000
VERSION     DATMPU010000194.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 8049)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8049)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8049
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1397286"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            492..1199
                     /locus_tag="VNY06_04485"
     CDS             492..1199
                     /locus_tag="VNY06_04485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012041810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HWX14107.1"
                     /translation="MSGLDPAFQKDALNLIIAVLLGGVIGFERQWRHRLAGLRTNTLV
                     SLGAAIFVVFARQFADESPTRVAAQVVSGIGFLGAGVIWKEGLTVRGLNTAATLWCSG
                     AVGLLAGSGHWPHAALSAGLVVGVNLLLRPLVTLINRQPIESAEIETSYVVTVTCRGL
                     DEAKIRALLVQGFGVSDLHLRELESTDIEGTDRVEVTATLTSQKRREIALEYIVGSLS
                     LDPSVTAARWRVVNSIV"
     gene            1534..2337
                     /locus_tag="VNY06_04490"
     CDS             1534..2337
                     /locus_tag="VNY06_04490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012589979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWX14108.1"
                     /translation="MNLLTPFANAWQHRELIRAVVRRELAVRFRGTILGWLWAIFGPL
                     VMLGAYTVVFSHAVGLPASAKGAGAGNYALSIFAGLIVFNLFSELAYRSPGLLHEHAG
                     VIKKSIFPSETLAWTAATRAAVYAAISLGVLVVFELALTWRLPLTAVLLPLIVAPLFL
                     FLLGISWFLMALGSFTRDVAHLMISIVPVFMFATPIFYSIEDVPPDLRLYLHLNLIGN
                     YVEMIRDLLLFSKLPELLLYCGTVVASILVFFFSYRFFMQYKAVFTDVI"
     gene            2327..3091
                     /locus_tag="VNY06_04495"
     CDS             2327..3091
                     /locus_tag="VNY06_04495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016917960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWX14109.1"
                     /translation="MSSDVVISCEHLGKAFQLYLRRNDQLMQALFGHWKQFYHPHWVL
                     RDISFKVRRGERIGIVGRNGAGKTTLLQIICGITLPTHGKVEVKGQVAPILALGAGFD
                     HELTGRENALIGGAVLGMRRATIEARLSAIATFADIGDFFYQPVKLYSSGMAARLAFA
                     VCAHADADILIVDEALSVGDAAFGKKCDAFIRDFGKRGTILVVSHDLDTLQTLCDRVI
                     WIEDGIARGMGPAAEILAAYRAAIGIKKAPALAASF"
     gene            complement(3333..3503)
                     /locus_tag="VNY06_04500"
                     /pseudo
     CDS             complement(3333..3503)
                     /locus_tag="VNY06_04500"
                     /inference="COORDINATES: protein motif:HMM:NF013412.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
     gene            complement(3656..4804)
                     /locus_tag="VNY06_04505"
     CDS             complement(3656..4804)
                     /locus_tag="VNY06_04505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012589976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HWX14110.1"
                     /translation="MPRLLFLYFSKRIALSSLLIATGLCVPIVMSSLFHYLPAAAIRG
                     GLLMPALLGTFPTVVYIVLPAAVGVAVALEFARMSVDGMIAVLYSLRLSAWAISLPAA
                     FVAMIAVACGYWVSIVFAPAHTGEMHDVIFVIRNSLNHRMLEPARFYSFDYGARTIYF
                     RRWISSDVVSGMFIHQYSSEKNEEQVILAEKAEFRRNAQGVVLIMSKGSIETIPADGT
                     TARSANFDEYAMPIGMQGSSGLPQRSWRGPFELSPVEFFRERPSAKMSPGPYAEWMSE
                     GAKRFGIPAIALGHALLAIGLVLSLGSATGRAPAGVLMAAVVAAPAVHIAILVGAETL
                     VRKDPRLVWLVGAAILAELAAAIFLIARQNANFSLPVDKARRAGLPGS"
     gene            complement(4835..5644)
                     /locus_tag="VNY06_04510"
     CDS             complement(4835..5644)
                     /locus_tag="VNY06_04510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020175033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX14111.1"
                     /translation="MATIANDKIIQWLLPFLESYLATNMSTPLYIIPYDDNVPLTRRA
                     AEIYGANFVEPDSADLDDLAKRLYPMFPAHRRRVRKFLSLALPLDEVIYFDADVILFR
                     DFSSMFGRLEAGKNDFIVAAQTKDYVYNKNSDQFDFLREAMLFNDGFFVASNSILGLR
                     DFLEVIERDEAIFHAVRQRGMLFAQPLTNFVVHRLGLRVTPLYKCIPGASGESFYKAE
                     GVTFDAEGPRDAMGDEIYFAHWAGAVTLPNRRIFDAAWRQYATRAAGRVKC"
     gene            5878..7230
                     /locus_tag="VNY06_04515"
     CDS             5878..7230
                     /locus_tag="VNY06_04515"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012589973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HWX14112.1"
                     /translation="MHRTGGARQNGFTCDPNRLDSIDEAAADPAVAFPRPLAGKIVAI
                     VHPAWHSCGSHGIFVSQVRAYRSLGAKAVSLALADTPGCTSNTRGSKAYFAATGELDA
                     DFRIFAGMPLRKVLNRSFLRAGRHWLHGNQAAMRLEVARFTPIPDVQNSVSRIDLLHC
                     NHFFSMPVAARLRERYGCPVILETHDLQARQYALHNRLGLRFPPATSYAAMLAIECAA
                     MSEADILLHLNAEEAAAFQELLPKKRHVLLYPAVGAMPTGAGGGEAIIVASANCPNFL
                     GLCWFLQEVLPLVPKIPVQIFGNIDRLFRWRAPGLLKKHAGLFQGRVEIAKLRDAYQN
                     ASAVLLPATAGHGISIKTIEALSCGAPLIATPLAFRGFPAGAANLPNVTIAEDAASLA
                     AALRRAHERRHQSELDRASSPTRRLYDQHFAFDTYCRSLCAIAEKLMKRETGRTGEDL
                     "
     gene            complement(7390..7620)
                     /locus_tag="VNY06_04520"
     CDS             complement(7390..7620)
                     /locus_tag="VNY06_04520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX14113.1"
                     /translation="MHVFEAFRFKPINPVLLWRAAHDINIAAGGLRALRVPKDTMVMA
                     ATLSAMFDPLKIDTPESFRSDRPWGVIFFGDM"
BASE COUNT         1658 a         2250 c         2353 g         1788 t
ORIGIN      
        1 aggtgcgaga tcgtaaaaat tgaggagctt ggtggtcatc gctcacctcc agcctttctt
       61 ggtgcggctg acggtgagcg ggaggggtga gccctaggct ggccctaaac gtctcaggcg
      121 atcagtgccg catatgtaac aattgggtga gacttgcccg gcatacgccg agcttgtacc
      181 accggagaag gtactgccca tttgtcctta aaggggttgc gcgaatctga actggccaga
      241 ccgcctaagc ccaaaatgcg ccttgcaccc ggatgtgtca accttcaaac atctttttag
      301 cctttccgaa tcgttgcgga ggcggttgct aatcagcagg cggggcgata gcaaacatgc
      361 gcccgccgcc ggccgtcacc agcgcctgtc gcgcagtcaa gaggcgaaag tttactgcaa
      421 gctttttgcc tgcccgggcc gcgctccgcg cataaccgca acaaaaggcc cggaggctga
      481 aaagtcccat aatgtcaggg ctcgatcccg catttcagaa agatgcgctt aatcttatta
      541 tcgccgtttt gctcggcggg gtcattggtt ttgagcgcca gtggcgccac cgcctcgcgg
      601 gtttacggac aaatacactg gtttcgcttg gcgcagcaat ctttgtggtt tttgcccggc
      661 aatttgcaga tgagagcccg actcgggtcg ctgcgcaggt cgtatcgggc atcggctttc
      721 ttggtgccgg agtaatctgg aaggaaggtc tcactgtgcg cggcttgaat accgcggcaa
      781 ctttatggtg ttccggcgcg gttgggctcc tcgccgggtc gggccattgg cctcatgctg
      841 cgctctccgc cggtctggtg gtcggtgtca acctcttgct gcggccactc gtgacattaa
      901 tcaaccgcca gcctatcgag agcgcggaaa tcgaaacgtc ctatgtcgtg accgtcactt
      961 gccggggact tgatgaagcg aaaatccgcg cgctgctggt gcaagggttt ggggtgagcg
     1021 atcttcatct gcgcgagctc gaaagcaccg atatcgaggg caccgatcgg gtcgaggtga
     1081 cagccacgct cacctcgcag aagcggcgcg agatcgcgct agaatatatt gtcggttcct
     1141 tgagccttga cccgagtgtg acggccgcgc gctggcgggt cgttaactcg atcgtttgac
     1201 gcagctgggc cgcagcggct cagggagggg atcgcggcgt tttttgacgc gcaactcggc
     1261 tggctggaac aagctatgaa aacccaagcg gggattgaaa atgggacgat cctacagggg
     1321 ttgcgccgcc cctagtgcga agctcgtgct ctcgacgttg gtgggaatgc tcctgctggg
     1381 gcggcttttt gataaacggc gataactatt gcagcgcttc tcaaatcctt gcgatgagtt
     1441 gcaagcgttg acgggcgcag cgcaaactgg tctacatcgc cgccatcggc cgcgattcgt
     1501 ctgcgcgacg ggttggatga gacttcactt cgcatgaatc tgctgacgcc tttcgccaac
     1561 gcctggcaac atcgcgagtt gatccgggcc gtggtcagac gcgagctggc agtccgtttc
     1621 cgcgggacga ttcttggctg gttatgggca attttcgggc ctctggtcat gctcggcgcc
     1681 tatacggtgg ttttttccca tgcggtcggg cttccggcgt cggcgaaggg ggccggagcg
     1741 ggaaattacg ctttgtcaat ttttgccggt ttaattgtgt tcaatctgtt tagcgaactc
     1801 gcctaccgct cgccggggct tttgcatgaa catgcaggag tcatcaaaaa gtcgattttt
     1861 ccgagcgaaa ccttggcttg gacagcggca acccgcgccg ctgtttatgc cgcgattagt
     1921 ctcggagtct tggttgtttt cgagctggcc ttgacctgga ggctgccgct tacggcggtg
     1981 ctactgccct tgatcgtcgc gcctctcttc ttgtttctgc ttgggatctc atggtttctg
     2041 atggcgcttg gatcgtttac ccgcgatgtc gcgcatctca tgatttccat cgtaccggtc
     2101 tttatgttcg ccacaccgat cttttactca attgaggatg tgccgcccga tttgcgcctc
     2161 tatctgcact taaatttgat cggaaactac gtcgagatga tccgggacct tcttttgttc
     2221 agcaaattgc ccgagctttt gctttattgc ggcacggttg tggcatcgat cctcgtgttc
     2281 tttttctcct atcggttttt catgcaatac aaggctgtgt tcaccgatgt catctgatgt
     2341 tgtcatcagc tgcgagcacc ttggcaaggc gtttcagctt tatttgcggc gcaatgatca
     2401 gctcatgcag gcgcttttcg ggcattggaa gcagttctat catccgcatt gggtgctgcg
     2461 ggacatcagc ttcaaggttc gccggggcga aagaataggt attgtcgggc gcaacggcgc
     2521 tggcaagacg acacttttac aaatcatctg cggcatcacc ttgccgacgc atggcaaggt
     2581 ggaagtcaag ggtcaggttg cgccgatcct agcccttggc gcaggcttcg atcacgagct
     2641 tacggggcgc gagaatgcgt tgatcggcgg cgccgttttg gggatgcgcc gcgccacgat
     2701 cgaagcaagg ctttctgcaa tcgccacctt tgccgatatt ggcgatttct tctatcagcc
     2761 ggtcaaactt tattcgagcg gcatggccgc gcggcttgcc tttgcggttt gcgcccatgc
     2821 cgacgcggat atactgatcg tggacgaggc tctgtcggtt ggcgacgcgg cctttggcaa
     2881 gaaatgcgat gctttcatca gggactttgg gaagcgcggc acaattcttg tcgtttccca
     2941 tgatctcgac acgctacaga cgctctgcga ccgtgttatc tggatcgagg atggcatcgc
     3001 gcggggcatg ggtcccgccg cggaaatcct cgccgcttac cgtgcggcga tcggaattaa
     3061 aaaagctccg gcgctcgctg caagcttttg attttggccg ggatgggacc tggtgacgtc
     3121 ttagcgtcag ccagaacgca ccctcgccta agcctttccc ttggcgagca gatcagcgta
     3181 ggcttcccgg gtgatttctc cgcccgcgtc cgggaccgtt tcgcgatcgc gtggcgggat
     3241 cgcatcttgt gctgtgtcca cccgtttccg ggccggcaat aagcgcttgc ggtaaatcca
     3301 ggcgcaccgc ccttgtaaga accaatcggc cggccaaatg cgtcgacggt cgaaagaaag
     3361 aacatgtcca gagattcgat gaacgcacgc acctgttcgg cgaattcgct gtgcacaata
     3421 cggttttcca tcatttccgc cacgcgccgc gtatcgaact tgtcctgcag cgcacgatgc
     3481 tcagctgtat aaaacttcga cattaatcga tccttcactt tttgtagctg tcgggagggc
     3541 aagatgactt ccttcgattc atcatctttc cccacgtcct tggtttgccg ctcgtccgga
     3601 cggaaaaccg gttccgcttt tggcccgaca cgttccggcg gggcccgttt ttctcttagg
     3661 aaccgggtag accagcccgc ctggccttat ccacgggcaa ggagaaattg gcgttttgcc
     3721 gtgcgataag aaaaatggcg gctgcgagct cggccagaat tgctgcgcct acgagccaga
     3781 caaggcgagg atcctttcgc accagcgtct cggcgccaac gagaattgca atatgaactg
     3841 ctggtgccgc gaccacagcc gccatgagga ctcccgccgg agcgcgcccg gtcgcagagc
     3901 caaggctcag gacgagtccg atcgcaagaa gcgcgtggcc gagcgcaatg gccgggatac
     3961 cgaaccgctt tgctccctcg ctcatccatt ccgcataggg gcccggagac attttggcgg
     4021 acggccgttc gcgaaaaaac tcgacaggag acaattcgaa cgggccccgc caactgcgct
     4081 gcggcagtcc ggacgagcct tgcatgccaa tgggcatcgc atattcgtcg aaattcgcgc
     4141 tgcgggccgt cgtgccgtcc gcaggaatgg tctcaatcga acccttgctc atgatcagca
     4201 cgacaccctg ggcattgcgg cgaaactccg ctttttcggc aagaatgacc tgttcctcgt
     4261 tcttttcgga agaatactga tgaatgaaca tgcccgagac gacatcggac gaaatccagc
     4321 ggcggaagta gatcgtccgc gcgccataat caaagctgta gaagcgcgca ggctcaagca
     4381 tccggtggtt gagcgaattg cgaatgacaa aaatcacgtc atgcatctcg ccggtgtggg
     4441 ccggcgcgaa aacgatcgac acccaataac cgcaagcgac cgcgatcatg gcgacaaatg
     4501 cggcaggaag ggagattgcc caagctgaga ggcgcaggga atagagcacc gcgatcatgc
     4561 catcgaccga catgcgcgcg aattcgaggg caacggctac gccaaccgcc gccggcagga
     4621 cgatgtagac gactgtcgga aatgtaccaa gaagcgcggg catcagcagg cccccgcgga
     4681 tagcggccgc cgggagataa tgaaaaagcg acgacatgac gatcggaacg caaagacctg
     4741 ttgcgatcag aagggatgat agcgcaatgc gtttcgaaaa gtagaggaaa agcagtcgag
     4801 gcatctcacg atccgggccg caattaccaa gagttcaaca cttcacccgc cccgcggcac
     4861 gggtcgcata ttgacgccag gccgcatcga atatgcggcg gttcgggagt gttaccgcgc
     4921 ccgcccaatg ggcgaaataa atttcatcgc ccatagcgtc gcgtggcccc tcggcatcga
     4981 aagtcactcc ttcggccttg taaaaactct ccccggacgc gcctggaatg catttgtaca
     5041 gaggcgtgac ccgcaatcct aaacggtgca cgacaaaatt ggtcaagggc tgggcgaaca
     5101 gcatgccgcg ctgacgtaca gcatgaaaaa tagcctcatc cctttcgatg acctcgagaa
     5161 aatctctaag gcctagaata gaattcgagg cgacgaaaaa cccgtcgttg aagagcatag
     5221 cctcgcgaag aaaatcgaat tgatcgctgt tcttgttata gacatagtcc tttgtctgcg
     5281 cggcgacgat gaaatcgttc ttgcccgctt cgagacgccc gaacatcgag ctaaaatcac
     5341 gaaacagaat gacgtccgcg tcgaaataaa tgacctcgtc aagcggcagc gcaaggctaa
     5401 gaaacttcct taccctcctc cgatgcgcgg ggaacatcgg atagagccgc ttggccaggt
     5461 catcgagatc ggcgctatcc ggttcgacga aatttgcgcc gtaaatctcg gcggcacggc
     5521 gcgtgagcgg gacattgtcg tcataaggga tgatgtatag aggcgttgac atattggtgg
     5581 cgaggtagct ctctaggaac ggcagaagcc attggatgat tttgtcgttt gcaatggtcg
     5641 ccattccata ctttctggtt ggaggagcag ttttttgcat ttaagccttg aatttatgag
     5701 gttctcggcc ctcgccttgg cggctatgga cgcggcattt tcattctgaa tggaatggtg
     5761 aagttggctg gaggcggaaa agacctgagt aagccataag gtctcgtctc gtgtagcttg
     5821 gcgagggcaa ttcgatcaag ccaaagcgtg ggcgagccgg cgaatgtgga aattgagatg
     5881 caccgaaccg gcggagcgag acagaacggt tttacttgcg atcccaatcg gttagactct
     5941 atcgacgaag ctgccgcgga tccggccgtg gcttttcctc ggcctttggc aggcaaaatc
     6001 gtcgcgattg tgcatccggc atggcattct tgcggcagcc acgggatttt tgtttctcag
     6061 gtgcgggcct atcggagtct cggcgcgaaa gcggtgagcc tcgcgcttgc cgatacgccg
     6121 ggatgcacta gcaatacgcg ggggtcgaag gcctatttcg cggcgaccgg cgagctcgat
     6181 gccgattttc gtatctttgc cggcatgccc ctgcgcaagg tactgaaccg cagttttttg
     6241 cgagcgggca ggcattggct gcacggcaat caagccgcga tgcggcttga ggtcgcacga
     6301 tttaccccca tccccgatgt gcaaaactcc gtttcacgga tagacctcct ccactgcaat
     6361 cattttttct ctatgccggt cgcagcgcgc ttacgagagc ggtatggctg tccggtcatt
     6421 ctcgaaactc acgaccttca agcgcgccag tatgcgctgc acaatcgctt aggattgcgg
     6481 tttccgcccg caacaagcta tgcggccatg ctcgcaatcg aatgcgcggc catgagcgag
     6541 gccgatattt tgctgcatct caatgccgag gaggcggccg catttcaaga gcttcttcca
     6601 aagaagcgcc acgtgttgct ttatcccgcc gtcggtgcga tgccaaccgg cgcaggaggt
     6661 ggcgaagcca tcattgtcgc cagtgccaat tgcccgaatt ttctcggact ttgctggttt
     6721 ttgcaagagg tgctgccgct cgtgcccaag attccagttc agatatttgg caatatcgat
     6781 cgcttatttc gctggcgcgc gccgggtctc ctcaaaaaac atgccggtct cttccaagga
     6841 agagtcgaga tcgcaaagct ccgtgatgcc taccagaacg cgtcggcggt gctgctacct
     6901 gccaccgccg ggcatggtat ctcgatcaag accatcgagg cgctctcctg tggcgcaccg
     6961 cttattgcga cgccgctcgc cttccgcggg tttccggcag gtgcagcaaa tctgcctaac
     7021 gtgacgattg cggaagatgc agccagttta gcggcggcgc tgcgccgcgc tcatgagcgg
     7081 cgtcatcagt ctgaacttga tcgggcgtca tcgcctacgc ggcgtttata cgatcagcac
     7141 tttgctttcg acacttattg cagatcgctg tgcgcgatcg cggagaaatt gatgaagagg
     7201 gaaaccggca gaactggcga ggatctttaa tcagaccatt cgacgagaaa atgtttggga
     7261 aattgcgtgc cgccagtgtc gatctggccg gcttcacccg gtgcacggcg aaggccgtgc
     7321 gttacaacca gcggttttaa gattgcggca caacggcacg gttgatatgg gcgccgaaac
     7381 atccatgcgt cacatatccc caaagaatat gacgccccaa ggcctgtcgc tgcgaaacga
     7441 ctccggcgta tctatcttca gcggatcaaa catcgcggag agagtggccg ccatcaccat
     7501 ggtgtctttc ggaaccctga gggcgcgcaa accgcccgcc gcgatattga tgtcgtgggc
     7561 agcacgccag aggaggaccg gattgatggg tttaaaccgg aatgcctcaa aaacatgcac
     7621 gcaccgccgc tggcacctgg tacaaagcgg cgagcccttc accgatcgtg cgaaagcgca
     7681 caatgagaac cgtgacaaat ttggcaagcc catcgatggt tccttcccgc tctcgcgaat
     7741 ctaggtaggg tcgactgcgc gtatgccgat catgcgcaac gcggtcctgc tcggcacgaa
     7801 gaaatattcg ccgccaagcg tctcgacgaa ttgcggaatg ccgtcgacga tgaatgggtc
     7861 cccccggggt tgctgcggaa agcaaatttt ctaagcgccg cgtagcgagc atttggatcc
     7921 gactctgccc agtgctccac cgtcggcgcg accgagagag gcacgccgtc tcgccaccgc
     7981 tcttgggcgt gcattggatt atcgatgccg aagtcagcgc caaagttttt cgctgtctcg
     8041 tttataaat
//
LOCUS       DATMPU010000195         5222 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_151033,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000195 DATMPU010000000
VERSION     DATMPU010000195.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 5222)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5222)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5222
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_151033"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1569)
                     /gene="glnD"
                     /locus_tag="VNY06_04525"
     CDS             complement(<1..1569)
                     /gene="glnD"
                     /locus_tag="VNY06_04525"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012591787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008773 - [protein-PII]
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0018177 - protein uridylylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[protein-PII] uridylyltransferase"
                     /protein_id="HWX14114.1"
                     /translation="MNARPETALPVPPRPERSASPQGAYFHRANLRAKIAAANAAGTS
                     AHAARAETLNHFRAAFEQGRAFVRQTFERDGGGLACAGRLAHLEDELIREIVRYVTSY
                     VQLTAIASTGEACAIAAVGGYGRASLAPGSDIDLLFILPPANRAHGERVVEPILYMLW
                     DLGQKIGHSTRTVDECLVDAREDMTIRTAVLEARFILGNHGLFDTLQSRFESEIVRKS
                     AAEFVAAKLAERDSRIAQAGRSRYLVEPHVKDGKGGLRDLNTLFWIAKYVYQLRQEAD
                     LIAVGVFSQSEYKLFRRCEEFLWRVRCHLHFLTGRAEERLSFDHQRRIADRLGYSTRA
                     GLASVERFMKHYFLVAKDVGDLTAIVCAALEERQAKPSARLDRFAPMHPRRTIVATGD
                     FEVENGRVTVKGHEVFGRDPVNLIRLFWVADRNSLAIHPDATRKVTLALKLIDARLRA
                     NPEANRLFLEILTSRNEPEIVLRLMNEAGVLGRFIPEFGRIVAFMQFNMYHHYTVDEH
                     LLRSVGNLAELEAQK"
     gene            complement(1566..2627)
                     /gene="gshB"
                     /locus_tag="VNY06_04530"
     CDS             complement(1566..2627)
                     /gene="gshB"
                     /locus_tag="VNY06_04530"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018045812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004363 - glutathione synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HWX14115.1"
                     /translation="MHHRPWRTTLNVAVQMDPIDKITIAGDSTFALLLEARARGHKLF
                     YYTPDKLSLRDNRVLADVAPIEVRDVEGDHFTLGETRTCDLSSLDVILLRQDPPFDIA
                     YITSTHLLEHLQPKVLILNDPASVRNAPEKLFVMNFPQLMPPTLITRSKSAIESFLDE
                     HGEAVMKPLHGHGGAAVFKIARNDRNFGSLFDLFAASFREPWVVQRFLPQVMQGDKRI
                     ILVDGEPGGALNRVPAKNDIRANMARGGGAAAAELTSREREICAVIGPELKQRGLLFA
                     GIDVIDGLLTEINVTSPTGIRAIKRLGGPDLAIAIWDTIEARLRTGLALPCADTATRS
                     ISLAEETQYLHSSLSHRNP"
     gene            2904..3824
                     /locus_tag="VNY06_04535"
     CDS             2904..3824
                     /locus_tag="VNY06_04535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018265679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HWX14116.1"
                     /translation="MKKAFEKSQNPCWQAARRRLTCVTAVAALSLLAWPPALADDSFD
                     PLKGIKNLFSGEKYETKIVPDVPAGDLYNQALAKLESKDYGGAAKKFEEIEKQYPYSQ
                     WQRKAILMMTFSQYQNGSYDDAIASAKRYIARFPAGPELDYAHYLEAMSYYNQIPDIS
                     RDQDRAAKAAETFSVIVEKFPKSEYASDARYKLQVARDQLAGKEMLVGRYYLNQHNYA
                     AAINRFREVLFKYQSTRHTEEALMRLTEAYLALGVTNEAQTAAAVLGHNFPESQWYKD
                     AFALLREGGLTPHEDEGSWISKAFRKMGLG"
     gene            4053..>5222
                     /locus_tag="VNY06_04540"
     CDS             4053..>5222
                     /locus_tag="VNY06_04540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012592455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HWX14117.1"
                     /translation="MLVQLSIRDIVLIDKLDLEFDRGLTILTGETGAGKSILLDGLSL
                     ALGARGEAGLVRHGATQGHVSAAFELTVDHPAIAAARAQDIETSGNLILRRLQLSDGR
                     SRAFVNDERVTTQALRVIARELVEIHGQHDDRALVNPSTHRQLVDAFGGLNAMVTEVR
                     DAFTRLRDLKTERAREQARLDVARSEADYLRHAFAELTELAAVPGEENTLAERRSAMM
                     QAEKVASELCDVRAAVTGEHSPMPALSAALRRLQRRQSQAPDLIAPAAEALDAVLNAL
                     DLAGQALDQAVRDADFEPRELERIEERLFALRAAGRKYAVQVDGLATLSEKFAADLAM
                     LDAGEQRLKQLTCEVDVAESAHGSAARALSEARKNAAARLDEAVGAELAPLKLEGA"
BASE COUNT         1142 a         1453 c         1536 g         1091 t
ORIGIN      
        1 cttttgcgct tctaattcgg cgagatttcc cacggaacgc aggagatgtt catcgaccgt
       61 gtaatgatgg tacatgttga attgcatgaa cgcaacgatg cggccaaatt ctgggatgaa
      121 acgcccaaga acgccagcct cattcatgag ccgcagcacg atttcaggct cgtttcgcga
      181 ggtcagtatc tccaaaaaca gtctgttcgc ttcgggattg gcgcgcaacc tcgcatctat
      241 tagtttgagc gccagcgtga cctttcttgt cgcgtcggga tggatggcaa ggctattgcg
      301 gtcggcgacc cagaaaagcc tgatgaggtt gacgggatcg cggccgaaga cttcatgtcc
      361 cttaaccgtg acacggccat tttcgacctc gaagtcgccg gtcgcgacaa ttgtccgccg
      421 gggatgcatt ggcgcgaacc gatcgagccg ggcggatggt ttggcttgcc tttcctcgag
      481 cgccgcacag acgatcgcgg tcaaatcgcc gacatccttg gcaaccaaaa aatagtgttt
      541 catgaatcgc tcaacgctgg caagaccggc tcgagtcgaa tagccgagac ggtcagcgat
      601 tcggcgctga tggtcaaagc tcagtctttc ctctgccctt ccagttagaa aatgcagatg
      661 gcaacggacg cgccacaaga actcctcgca gcggcgaaac agtttgtatt cagattgcga
      721 aaacacgccg actgcgatta ggtcggcctc ctgacgcagt tgatagacgt atttggcgat
      781 ccagaacaaa gtatttagat cccgcaggcc gccttttccg tccttgacat gcggctcgac
      841 gagatagcgc gagcgaccgg cctgggcgat gcggctatcg cgttcggcta gctttgccgc
      901 gacgaattcc gccgcggatt tcctcacgat ctcgctctca aaccggctct gcaatgtatc
      961 gaaaagtccg tgattgccga ggataaaacg cgcctcaaga accgcggtgc ggattgtcat
     1021 gtcttcgcga gcatcaacga gacattcgtc gaccgttcga gttgaatggc caatcttctg
     1081 accgagatcc caaagcatat acagaattgg ctcgacgacg cgttcaccat gcgcgcggtt
     1141 agcgggaggc aaaataaaca aaagatcgat gtcagaaccc ggggcaaggc ttgcccggcc
     1201 atagccgcca acggcggcga ttgcgcaggc ctcgcctgtc gaggcaatcg cggtcagttg
     1261 aacataggat gtcacatagc gaacgatctc tcggatgagt tcgtcttcga gatgagcgag
     1321 gcggccggcg caggcaagac cgccgccatc gcgctcaaat gtttgacgaa caaacgcgcg
     1381 gccctgttca aatgcagcgc gaaaatgatt gagcgtttcg gcccttgcgg catgtgcgct
     1441 tgtgcctgcc gcattggcgg ccgcgatctt cgcccgtaaa ttcgcgcgat gaaaataggc
     1501 tccctgtggg gaagcgctac gctcgggccg gggaggaacc ggcagtgccg tctctggtct
     1561 cgcgttcatg gattccgatg ggacagggac gagtggaggt actgcgtctc ttccgctaag
     1621 ctaatcgatc tggttgcagt atccgcgcaa ggcagggcca agccggtccg taaccttgct
     1681 tcgatcgtgt cccaaatcgc gatcgcgaga tccgggccgc caagccgctt gatcgcgcgg
     1741 atcccagtcg gtgaggtcac attgatttcc gtcagaaggc cgtcgatgac gtcaattccc
     1801 gcaaaaagca gtccgcgttg tttgagttcg ggcccgatga cggcgcagat ctcacgctca
     1861 cgcgacgtga gctcagccgc cgctgctccg ccaccacgtg ccatgttcgc gcgaatgtca
     1921 ttcttggcag gtactcggtt gagcgcaccg ccgggctcgc cgtcgacgag gatgatgcgt
     1981 ttatcgcctt gcatgacctg cggcagaaag cgctgaacca cccatggttc gcgaaaactc
     2041 gccgcaaata gatcaaacag cgaaccgaag tttcgatcat tgcgggcgat cttgaacact
     2101 gccgcgccgc catgcccgtg caaaggtttc atcacggcct caccatgttc gtccagaaag
     2161 gattcgatgg ctgacttgct tcgggtgatt aacgtcggcg gcataagttg aggaaagttc
     2221 ataacaaaaa gcttctctgg tgcattgcgc acgctggccg gatcattgag gatcagaact
     2281 ttcggctgca aatgctcgag gagatgcgtg gaagtgatgt aggcgatatc gaaaggcgga
     2341 tcctggcgca gaagaatgac atctagggaa gagaggtcac acgttcttgt ctcgcccaag
     2401 gtaaaatggt cgccttcgac gtcgcgtact tctattggag ccacatcggc gagcacccgg
     2461 ttatcacgta gcgaaagctt atcaggtgtg tagtaaaata gtttgtgccc gcgcgcacgc
     2521 gcctcgagca gcagcgcgaa ggtggaatcg cccgcgatcg tgatcttgtc gatcggatcc
     2581 atttgaacgg ctacgttcaa ggtcgtcctc cacggccgat ggtgcaaatt ctcgcccgcg
     2641 gcatgcacat ggcccgggag attcgtcaag ggcgcccggc accaacccag actaagtccc
     2701 gtatcgtggg cggctcggct tggaaaaaag ctgacaggcc ccctctggcc gtatatggcc
     2761 ttgctgatgt tctttgccaa ttgagaccat tctgccacat ttatgctttt ttgccgaccg
     2821 acatggaagc ctcgccgtgg caccggttct gacagaacgg ttgcgcccct tacagatctc
     2881 tttgcgacga agggtcagag cgcatgaaaa aggcctttga gaaatcgcaa aatccctgtt
     2941 ggcaagccgc gcgcaggcga ctgacctgtg ttacggcggt agcggcttta tctcttctcg
     3001 catggccccc ggcgttggct gatgattcct tcgatccgct aaaagggatc aaaaacctgt
     3061 tcagcgggga aaaatacgaa accaagattg tgcccgatgt cccagcgggc gatctctaca
     3121 atcaggcgct tgccaagctg gaatcgaagg actatggcgg cgcggccaaa aagttcgagg
     3181 agattgagaa gcagtatccg tattcgcagt ggcagaggaa ggcgatattg atgatgacct
     3241 tcagccaata tcaaaatggc agctatgacg acgcaattgc ctcggcgaaa cgctacattg
     3301 cgcggtttcc tgccggcccc gagctcgact atgctcacta tctcgaggca atgtcctact
     3361 acaatcaaat accggatata tcccgtgatc aggaccgtgc cgcgaaggcc gctgaaacat
     3421 ttagcgtgat cgtcgagaaa ttcccgaagt cggaatatgc ctcggatgca cgctacaagc
     3481 tgcaagtcgc ccgcgatcag cttgccggaa aggagatgct cgtcggacgc tactatctca
     3541 accaacataa ttatgcggcg gcgatcaatc gcttccgcga ggtcctattc aaatatcagt
     3601 cgacccgcca cacagaggaa gctctgatgc ggctgaccga agcttatctc gcgcttggcg
     3661 ttaccaatga ggcgcaaaca gcggcagcag tattggggca taattttccg gagtcgcaat
     3721 ggtacaagga tgcctttgct ttacttcgcg aaggcggatt gacgccgcat gaggacgagg
     3781 gatcctggat ttcaaaagca ttccgcaaga tgggcctcgg gtagatcgat caggatcggg
     3841 tggttggaac ggctttgata gattgaggct tttggccctt ttggcggtcg gagccccctt
     3901 gattcgaagc agttttgcgt cccggtcata agctgcatcc gaaaggatca cgcgtctatc
     3961 gagcacccgc aagggaccgg acctgttttc ggggaacatg aaagagctcc cgcagcgcca
     4021 ggaaacggcg tatcggcaag cgactcggtc ccatgctcgt gcaattgtct attcgcgaca
     4081 ttgttttgat cgacaagctc gatcttgagt tcgaccgcgg gcttaccatt ttgactggcg
     4141 agacgggagc tggcaagtcc atacttctcg acggactgtc gctggcgctt ggggctcggg
     4201 gagaggctgg tctcgtccgc catggcgcga cgcaaggtca cgtcagcgcg gcatttgaac
     4261 tcaccgtaga tcaccctgcg atcgcagcgg cgagggcgca agatatagaa accagcggca
     4321 accttatttt gcggcgccta cagttgtcgg atggccgtag ccgggccttc gtcaacgatg
     4381 aacgtgtcac gacgcaagcg cttcgggtaa tcgctcgcga gctcgtagaa atacatggac
     4441 agcacgatga ccgagcgctt gtaaatccga gcacgcatcg ccagctcgtc gatgccttcg
     4501 gtggcttgaa cgctatggtc accgaggtgc gcgatgcctt tacaaggcta cgtgatctca
     4561 agactgagcg cgcgcgcgag caagcgcggc tcgacgtcgc gcgcagtgag gccgactacc
     4621 tgcgccatgc ctttgccgaa ttgaccgagc ttgcagccgt gcccggcgaa gagaatacgc
     4681 tcgccgagcg ccgctccgcc atgatgcaag cggaaaaggt tgcctccgaa ctttgcgatg
     4741 tgcgtgcggc cgtgacaggc gagcattcac caatgcctgc tctttccgcc gcgttgcgta
     4801 ggcttcagcg gcgccaatcg caagcgccgg acctgattgc acctgcagcc gaggcgcttg
     4861 atgccgtatt gaatgctctc gatcttgcgg ggcaagccct cgatcaggcg gtgcgcgatg
     4921 ctgattttga gccacgagaa ttggagcgga ttgaggaacg actatttgcg ttgcgcgccg
     4981 cgggccgcaa atacgcagtg caggtagatg ggcttgccac gcttagtgaa aaattcgctg
     5041 cggaccttgc catgcttgat gccggggaac agcggctgaa gcaattgacg tgcgaggtcg
     5101 acgttgcgga atctgcgcat ggatcagcgg cgcgggcatt gtccgaggct cggaaaaatg
     5161 ccgcggcccg gctcgacgaa gcggttggtg cggaactcgc cccgctcaaa ctcgaaggcg
     5221 ct
//
LOCUS       DATMPU010000196         4173 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_778969,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000196 DATMPU010000000
VERSION     DATMPU010000196.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 4173)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4173)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4173
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_778969"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..392
                     /locus_tag="VNY06_04545"
     CDS             <1..392
                     /locus_tag="VNY06_04545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012590259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HWX14118.1"
                     /translation="QIAFIGSIAGIRGLPYSPAYCATKAAVHAYSESLRGRLEARGVR
                     VSLIIPGFVKTPLNDSIVAIKPLEITDVQAARLIQRGLEKSKSVIAFPKLLYLLSRFA
                     RILPVRIVDKIMAGIEVTIPQTRERNS"
     gene            695..2020
                     /locus_tag="VNY06_04550"
     CDS             695..2020
                     /locus_tag="VNY06_04550"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012590258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HWX14119.1"
                     /translation="MDFVGLFNILCDIGLIAAISGLLFVGVSFLILIGINIFEQATGR
                     LLGKPLPEARLHENDLPHVLVQIPVFNEPAMVAGCLRSVAALEWPRAKLHIQLLDDST
                     DETTSIAASVVFELRQQGFDVLHLRRVGRRGYKAGALAAGLAHSHAPCVAILDADFRP
                     PANWLREVVPALIADPAASFVQSRCEFANYRTNWLTRAQGLMFDAHFVIEQATRYRAG
                     WLFQFNGTGAVWRRAAIDAAGGWSGDSLCEDLDLTVRAEIAGWHGLFSMDPPVPGLVP
                     DRVAHWRVQQRRWSNGFVQVARKLLGEVWTADWRVWRKLSAMFLILIQMFYPCVALGS
                     LSLAFGAIVRGGEMAPYQPVIATIAATILTIGVGLTLMPYLILRRGPLRRYFATLASL
                     IPLMIYVSLSNAPSILKTVLGASDSWKRTPKTLHVSDVLPATDQLNEAS"
     gene            complement(2127..3095)
                     /locus_tag="VNY06_04555"
     CDS             complement(2127..3095)
                     /locus_tag="VNY06_04555"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006019237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HWX14120.1"
                     /translation="MASFIPTTLPRPHPPRRRAILAAHPEVAGLIGHDRRTAAITLCV
                     VAGQTAIAAGFGFLGPSYWWAALLAAFCIGAFANHAMFVVIHDATHNCIFKSNSLNKW
                     TAILADLPNTVPTAMGFRCYHMKHHSHLGDYDYDADLPSHWEARLFGRTAIGKAAWMF
                     IFPIFQLTRLGRLKGSVPMWGRWTFVNAGCVILYDLAVFLCFGPNALLYLFASFWFSV
                     GGLHPLGARWLQEHYTSESSQETFGYYGALNLVALNIGYHNEHHDFPDVPWSSLPALK
                     EMAPDFYDGLKTHKSWCGLLVKFIFDPQFTLYTRVDRSIARQAKAR"
     gene            complement(3298..>4173)
                     /locus_tag="VNY06_04560"
     CDS             complement(3298..>4173)
                     /locus_tag="VNY06_04560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012590387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX14121.1"
                     /translation="DAIDDDAVVAGLTRAAEEWLRQRGALRMHGPFSPSINSESGLLV
                     EGLREIPMIAVPWHPSYLGPSLERQGYAKARDLVSYRYDVGEKDRVAERGMIARPDWR
                     KRLNIKAIDIKNLKEEAKTIVDIFNDSWSENWGFVPFTLDEFLSSTGALKLVAPSDRG
                     FMKGGFMIEVDGMPQAFALVLPNLHEIMADLGGRLFPFGVLRLISRIRSHGYRSGRLA
                     LFGIRRALHGKIAGGAVILAFIAELRRRSVLSPIEQVEFGWVLADNQRMRRPIELSGG
                     HVYKVHRIYEKNLAA"
BASE COUNT          914 a         1181 c         1150 g          928 t
ORIGIN      
        1 gccagattgc attcattggt tccatcgcgg gcattcgcgg tttgccctat tctcccgctt
       61 attgcgcgac caaagccgcg gttcatgcct atagcgaatc gctgcgcggc cgccttgagg
      121 cccgcggagt ccgcgtcagt cttataatcc cgggattcgt gaagaccccg ctgaacgaca
      181 gtatagtcgc aataaaaccg ctcgaaatca cggacgttca ggccgcccgg ctcattcagc
      241 gcggtctcga aaagagcaaa tccgtcatag cgtttccgaa gttgctctat ctcttgtcgc
      301 gttttgcgcg cattctgccg gtccggattg tcgataagat catggcaggt atcgaggtca
      361 ctattccgca gacgcgggaa cgtaattctt aacaaaaact tacttttgta ccgaaacgcg
      421 gacacttttt cgccaaaatt gaaagtaatc ggcctttcca ctcaatttct gcaataaatt
      481 gttgcaaatt gtttgttgtc gaaacctcct gtttaaggtt caaagcttga gtgctacggg
      541 ctattttggc cgatgtagac aattggttcg cttgctcgaa tcagccgcac gtcaaattga
      601 cagccgcggc agaacggcca cgaagcgcca aaagacgcat gaaaatgcgc agggcaagtg
      661 gctcggacga gagaatagca cgggggcttt gctattggac ttcgtcggcc tattcaacat
      721 cctgtgtgac atcgggctaa ttgccgccat cagcggtctt ttgttcgtcg gcgtcagctt
      781 tctcattctc atcggcatca atatctttga gcaggcgacc gggcgcctgc ttggcaagcc
      841 gctcccagaa gcccggctgc atgaaaatga ccttccgcat gttctcgtcc agatcccggt
      901 ctttaatgaa cccgctatgg tcgccggttg tctgcgatcc gttgccgcac tggagtggcc
      961 acgcgccaag ctgcatatcc agcttctaga tgacagcacc gacgaaacaa cgagcattgc
     1021 cgcctcggtt gtttttgaac tgaggcagca gggttttgac gtcttgcatc ttcgccgcgt
     1081 aggacggcgt ggttacaagg cgggtgcgtt ggccgcaggc cttgcgcact cccatgcgcc
     1141 ttgtgtcgct atcctcgatg ccgattttcg cccgcctgcg aattggctgc gggaggtcgt
     1201 tccggcctta atcgccgatc cggctgcgag cttcgtacag tcgcggtgcg aatttgcgaa
     1261 ctaccggaca aattggctga caagggcgca gggcctcatg ttcgacgccc atttcgttat
     1321 cgagcaggca acccgctatc gagccggctg gttgttccaa ttcaacggta cgggcgctgt
     1381 ctggcgccgc gcggccatcg atgcggcggg cggctggtcg ggcgattccc tctgtgagga
     1441 ccttgatctt acggtgcgcg cggaaatcgc cggctggcat ggtttgtttt caatggatcc
     1501 cccggtgcca ggacttgttc ccgatcgggt tgctcattgg cgcgtgcaac aaaggcgctg
     1561 gtcaaacgga ttcgtgcagg ttgctcgcaa gctccttggg gaagtctgga cggccgactg
     1621 gcgggtttgg agaaagctgt cggcaatgtt tctcattctc atccagatgt tttatccttg
     1681 tgttgcgctc ggctcccttt ccttggcatt cggcgcaatt gtccgcggcg gcgagatggc
     1741 cccttaccag ccggtcatcg caactattgc ggccaccatc ctgactatcg gcgtcggttt
     1801 gactctcatg ccctatttga tcctgcgccg agggcctttg cggcgctact tcgccactct
     1861 cgcgtcgctc ataccgctga tgatctatgt cagcctgtca aatgcaccgt cgatcctgaa
     1921 gaccgttctc ggggccagcg actcctggaa gcgtacgcct aagacgctgc acgtatcaga
     1981 cgttttgcca gcgaccgacc agctcaacga ggcgtcttag cgctagaaag cccgcatctc
     2041 cggacgaaaa acctactttt gctggatagg ctcgagatcc ttattttgcc gcgagtccaa
     2101 acgcaaaacc ggttctcgct tttggctcaa cgcgctttag cctgcctggc gatactgcga
     2161 tccacccgcg tgtataaagt aaattgcgga tcgaagatga atttgaccag cagcccgcac
     2221 catgatttgt gcgttttgag gccatcataa aaatccggcg ccatttcctt taacgcgggc
     2281 aagctgctcc aaggaacatc tggaaagtcg tgatgctcgt tgtgatagcc gatgttgagc
     2341 gcgaccaaat tcaatgcgcc gtaatatccg aaggtctcct gggaagactc ggaggtataa
     2401 tgctcctgaa gccagcgcgc cccgagggga tgcagacctc ccactgaaaa ccagaaggag
     2461 gcgaataggt ataacagcgc attcgggcca aagcacaaaa ataccgcgag atcgtagaga
     2521 ataacgcagc ctgcgttgac gaaagtccac cggccccaca tcggcaccga gccttttagg
     2581 cggccgaggc gggtaagctg gaaaattgga aaaataaaca tccatgccgc tttgccgata
     2641 gcggttcggc caaataggcg cgcttcccaa tgactcggaa ggtcggcgtc atagtcataa
     2701 tcgccgagat gggagtgatg cttcatgtga tagcaacgaa atcccatggc ggtcggcacg
     2761 gtgtttggca gatcagcgag tattgcggtc catttgttca gagaatttga tttgaatatg
     2821 caattatggg tcgcatcatg aatgacaacg aacatggcgt gattggcaaa ggcgccaatg
     2881 cagaaggctg cgagcaaggc cgcccaccaa taggaaggcc cgagaaaacc gaagccggcg
     2941 gcgatcgctg tctgccctgc gacgacgcaa agcgtaatcg ccgcagtccg ccgatcatgg
     3001 ccgatgagac cggcaacttc aggatgcgcc gccaaaatgg cgcggcggcg cggcggatgc
     3061 ggacgtggca gcgtagttgg aataaaacta gccatgcgct ctccatcgat aggacgaccg
     3121 tctgcaggat gtaatgcgtc agttcgcatg cttagatcgc cctttgatac ggcccggact
     3181 cccggctcca tcgtcggcga cgattgcgcg tggtcttcgc gagaaggact cgagttgatc
     3241 atttgcatcg cgtccggagg aaccatagcg actttcacca aagacgaggt taccaaatca
     3301 ggcggcaaga tttttttcat agatccgatg caccttgtag acatgcccgc ccgagagttc
     3361 gataggccgc cgcatccgtt ggttgtctgc caaaacccag ccaaattcaa cttgctcgat
     3421 tggactcaaa acactgcgcc ggcgcaattc ggcaatgaag gccaaaatga ccgcgccgcc
     3481 ggcgatcttg ccatgcaggg cacgcctgat gccaaagagg gcaagccgcc cggacctgta
     3541 tccgtggctt cggatccgtg agattaaacg cagcacgcca aaaggaaaca gacgacctcc
     3601 gaggtcggcc attatttcat gcaagttcgg aagaacaaga gcgaaagcct gcggcatacc
     3661 gtcgacttcg atcatgaacc cccctttcat aaacccgcga tcagagggtg cgaccaattt
     3721 gagcgccccg gtactcgaca aaaactcgtc gagcgtaaag ggcacaaagc cccaattctc
     3781 actccaggaa tcattgaata tatcgacgat ggtctttgct tcctctttga gatttttgat
     3841 atcgatggct ttgatattga gcctttttct ccaatcgggc cgggcaatca tgcctcgctc
     3901 agccacgcgg tccttctcgc cgacatcgta gcggtaggaa acgagatctc gcgcctttgc
     3961 gtagccctgt cgttccaaag acggacctaa ataagatggg tgccagggca cagcgatcat
     4021 ggggatctcg cgaagtccct cgacaagcag gcccgactcg ctattgatcg agggcgagaa
     4081 tggcccatgc atccgcaagg cgccgcgctg tcgcagccac tcttccgcgg cacgcgttag
     4141 accggcgaca accgcatcat cgtcgatggc gtc
//
LOCUS       DATMPU010000197         3862 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_458115,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000197 DATMPU010000000
VERSION     DATMPU010000197.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 3862)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3862)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3862
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_458115"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..236
                     /locus_tag="VNY06_04565"
     CDS             <1..236
                     /locus_tag="VNY06_04565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX14122.1"
                     /translation="VLRNESFTDVLGQTDRGRFSGVARSERLDGREVSALWSRRAALV
                     AASACRRIGGLERGTGGAALFIGGPGRNAGSGF"
     gene            342..587
                     /locus_tag="VNY06_04570"
     CDS             342..587
                     /locus_tag="VNY06_04570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX14123.1"
                     /translation="MQHFYFHFRVGDRLIADEEGEDLPSLLVAQREAMRTARELLAEA
                     IKSGRPEIPDLVVIADETGRELCTIPLAMVLPEPLKH"
     gene            1159..1548
                     /locus_tag="VNY06_04575"
     CDS             1159..1548
                     /locus_tag="VNY06_04575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX14124.1"
                     /translation="MTSGMANALDEKEADFARSVTGMLFLTMICKDYAPQSEVLKYLG
                     KRSLGEDKSKKVLLAVGAELTKTLRAGGMKLEDGDAAAADLIPEISRIVDENISGLMD
                     LWRKDKDKTCRDVGDIAVTNGLAEKAK"
     gene            complement(2368..2539)
                     /locus_tag="VNY06_04580"
                     /pseudo
     CDS             complement(2368..2539)
                     /locus_tag="VNY06_04580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014332786.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
     gene            complement(2660..3127)
                     /locus_tag="VNY06_04585"
     CDS             complement(2660..3127)
                     /locus_tag="VNY06_04585"
                     /inference="COORDINATES: protein motif:HMM:NF016041.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HWX14125.1"
                     /translation="MIAMVYFGEMLVASLLAIVLLAISQLRMSSDAVLFAGGVVALTL
                     AEYLVHRFVLHGFAPTEHRLHHANPDGAVLTIFWQIWICFALVYLIAGGAFVAGALVA
                     YAWYLFVHHCAHHGSDKLRLPLLKHHQSHHRFASRNYGVSTTLWDHVFGTMLR"
     gene            complement(3196..3363)
                     /locus_tag="VNY06_04590"
     CDS             complement(3196..3363)
                     /locus_tag="VNY06_04590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX14126.1"
                     /translation="MKIVLRVLGPLFIAVGLFWFARGTGLFQGLHDAAMIDYGAGAVA
                     LGIGLVWFGWR"
BASE COUNT          873 a         1095 c         1130 g          764 t
ORIGIN      
        1 gtgttctgcg gaacgaaagt tttaccgatg ttctcggcca gacggaccga ggccgatttt
       61 caggcgtggc gcgatcagag agattggacg gccgagaagt atcggcgctt tggtcgcggc
      121 gagccgcttt ggtcgcggcg agcgcatgcc gccggattgg cgggctggag cgagggaccg
      181 gtggtgcggc gctgtttatc ggcggacccg gtcggaatgc tggaagcggt ttctaggccg
      241 ctagacgatt gaagcaggtc gcacccacta atgtgttgcg ccgaacagac gatgcaacag
      301 gcttcgccta agctctcttg ccggtcgtag gacgatatgc aatgcaacat ttctatttcc
      361 atttcagagt tggagatcgt ttgatcgccg atgaagaagg cgaggatttg ccgagtcttt
      421 tagtggcaca gcgtgaagcg atgcggacag cccgcgagct tttagccgag gctatcaaga
      481 gcggcaggcc agaaataccg gatctcgtgg tgatcgccga tgaaaccggg cgagagcttt
      541 gcaccatacc gctcgcaatg gttcttccgg agccattaaa gcactaaggc tgacttcatg
      601 ctgtacgtta ccgcgcgccc ggtggctgaa cgaggctgga aacgatcatt cgacaatcca
      661 atactgctgc ctcgcgggcc gccagctcgt caccttggac cgcgtgaatc cttgcttgat
      721 cacgcctgcc gtaggcgcat atttggagaa tgaccgggcg ggaccattgg accgaaagcc
      781 tcgtttgccc gaaatgcgga aagactggca tcgcccaatt ttcgcaagtg agcgggcgcg
      841 cataccccga cggcgatcaa gacatccgtg tcgatctcgc tcccatcggc ttcaaggctg
      901 tctcgaccga attcggcagc agcttttact gcgctagttg cggcacctct gcggatcaca
      961 aatcggtacg ctggaattga ccgcgcgcgt gtgcgcaagg ggcggaaccc gaaaatatta
     1021 agggccgctt tcgaccggca ttcggggcta gcatcgagcg tttttggagg gattggaaac
     1081 atgacagaag ttgtacaggt gcgagcccgt ggccacccgg taagagcgtg caccgtagga
     1141 gtcgcgctag cactgctcat gacaagcgga atggccaatg cgctcgatga aaaagaggcc
     1201 gacttcgcgc gatccgtgac cggcatgctt tttcttacaa tgatatgcaa ggactacgct
     1261 ccgcagtctg aggttctgaa ataccttgga aagcgcagct tgggtgaaga caaatcaaaa
     1321 aaggttcttc ttgctgtcgg tgccgagttg accaagacgc ttcgcgcagg cggcatgaag
     1381 ttggaggatg gtgatgcggc ggcggctgat cttattcctg agatcagcag aatcgttgac
     1441 gaaaacattt ccggcctaat ggacctctgg cgcaaagaca aagataaaac ctgccgcgac
     1501 gttggcgata tagcggtaac gaatggcctc gcggagaagg ccaaatagcg catcccccgc
     1561 gcccgcgcga ggcctcgccc atgcggcggc gcggatcggt cgcggttgaa tggcggcgga
     1621 tcagaggctc tcagccgggc tgcgccgggg cttctcgtcg ggaagaaacc cccggcggat
     1681 tgtcctgcat tgtgctcatg tcgactgcca cgcatttgac gtagtcgcag tcggaacaca
     1741 tgaaggtgta gaggtcgaaa tcaggccgtt cgggcatgat gcgaccgagc atcgtcggca
     1801 ctcggcactt cgggcacgga ctacccttaa tttcaacggc tgttgaatat gcggctagca
     1861 tggctccatt ctgacgcgtt cacgtcagcg ccaccgtcca atattgctca ctctcaacaa
     1921 tcgacaggca gttcagccaa tcctcctgag caaaattgag cagcggcggc gaaccggatg
     1981 tgatcggctg gtagccgaac gtcgcgggcc gctaccgcgc cgtttcccct agatggcccc
     2041 agggtcgccc cccgaacttg gggccgttta agcggacctt taagcgatca ggcgcgttga
     2101 ggccttgaga accggggcga cgacaaaaac gcggagcttt ggttatgagc agtattcctc
     2161 ttcacataga aagaaggttc gagcagcggt gggtcgctag atttttctcg ccggtcgcat
     2221 cggccgcgcc caaaagcacc gacttgaaag gcaccgtcaa cagcttgccg cgccctgcaa
     2281 tagcaaaaga aaaacccgcc gatgcgaagc ggcggcctcc aggcgacggg gatttagccg
     2341 atctttagtt tttccgccga cgttttgccg cgggttttcc atctcttcgt agcttacttt
     2401 ggcaccctca ttgagggtgc tcaggccggc tctttccacg gccgagatgt gtacaaacac
     2461 gtctttgccg ccgtgatcgg ggttgaataa atccaaatcc cttagtcgcg ttgaaccact
     2521 tcacagtacc tgtcgccacg tccgcactcc cttttttaaa cgatggcgac agcctagatc
     2581 ggtcgcgtac tagaccgcaa gacaaacccg ccggcggatc ggcgggctgg ctcgatgacc
     2641 agtcggtgac cccaaatcat cagcgcagca tcgtcccgaa tacatggtcc caaagcgtgg
     2701 tgctgacgcc gtagtttcgg gaagcaaatc tgtgatggct ctggtgatgc ttgagcagtg
     2761 gtaaccgcaa tttatcggaa ccgtgatggg cgcagtgatg cacgaacaaa taccaggcat
     2821 aggcaactag cgcgcccgca acgaaagcac cgcctgcgat caaatagacc agggcgaaac
     2881 aaatccatat ttgccaaaaa attgtgagga ccgcgccgtc gggattggca tgatgtagcc
     2941 gatgttcggt tggtgcgaaa ccatgcagca caaagcgatg cacgagatat tccgcaaggg
     3001 tcaacgcaac gacaccaccg gcgaaaagca cagcgtcaga actcattcta agctgcgaaa
     3061 ttgccagcag gacgattgcg agcaaggacg ctaccaacat ttcgccgaaa tagaccatgg
     3121 cgatcatgag cccagtctag gcgctagacc atcttcggcg caacgcccgg cgtgaaccga
     3181 tgtgagcgcg agcatctacc gccatccgaa ccagacaagg ccgataccta aggctacggc
     3241 gcccgcgccg tagtcgatca ttgcagcatc gtgcagccct tggaacaatc ctgtccctcg
     3301 tgcgaaccag aaaagcccaa cagctataaa tagcggacca aggactcgaa ggacgatctt
     3361 catgacggca cccttcgatc gcaaaaaatc aattacagcg atattgatcg aggctcagcg
     3421 agacgcaata aaaagccgcc cgggcatgac gccgagcggc tctatatgcg actgccggtt
     3481 tcccagttcc gccgctgcca tcaacaacgg tcgtgcggcg cgaaggttcc gcaagccaaa
     3541 cccgccggcc ggcgaccggc ggccatcaca atcgttctcg cgtgacctag ttcacatgcg
     3601 ctgagcagcg gctagcaatc accagtcggc cccatgaggc cactaccgga gatcgcaaaa
     3661 tggctgctct cgcgagatct ctttccaccg cgtccggcgc agacgtcggt gtggaaacgt
     3721 taaaaaccat cggcatgttg tgcggcgccg gtctattcgt attgctgctg ttcgcaagct
     3781 acggcttgga cctaagtccc ggctttttct gatatgaggg aaaggcccgc cggcgtgctc
     3841 ggcggcctac atccgtcaag gt
//
LOCUS       DATMPU010000198         2332 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_3871970,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000198 DATMPU010000000
VERSION     DATMPU010000198.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 2332)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2332)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2332
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3871970"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..451)
                     /locus_tag="VNY06_04595"
     CDS             complement(<1..451)
                     /locus_tag="VNY06_04595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012591939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HWX14127.1"
                     /translation="MSDMRLVVAGAAGRMGRVLVEIIRQTPGAHLAAAIEQPDSIAIG
                     QDAGLLAGCGQMGVVISGDALTAVAVADGILDFTSAQSTVELAALAAQARIVHVIGTT
                     GLNPSDLTKLDAAARHAVIVRSGNMSLGVNLIAALVEKAAHTLRPEYD"
     gene            complement(700..1119)
                     /locus_tag="VNY06_04600"
     CDS             complement(700..1119)
                     /locus_tag="VNY06_04600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX14128.1"
                     /translation="MNRPATAPHDTAAARKKLIIALDFEMQREAEAVVPILGNGARFY
                     KIGLELAYGGGLSLARDDSKRQEGLSRSQAARYRSHGEPGRPRNLPRGARRLPLLGVT
                     VLTSYNAEDLAEAGYAYGVADLVARRAAQAREAAPMR"
     gene            complement(1116..1934)
                     /locus_tag="VNY06_04605"
     CDS             complement(1116..1934)
                     /locus_tag="VNY06_04605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012591937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA adenine N-6-methyltransferase family
                     protein"
                     /protein_id="HWX14129.1"
                     /translation="MELRFADEARFFKSWLDNPGIAGAVSPSGPFLARMMARYVDPRK
                     KGPIVELGPGTGAITEALLERGIAPQRLFLIEYDPEFCCLLRRRFPGVHVIKGDAYQL
                     SKTLQGRLRRPPPAIVSSLPLLLKPESQRLALLAEASGCMAPDGCFVQFTYSPLSPIP
                     RDKLSALALHAVASPPVWLNLPPARVWIYRRMTGADTQLKLKRPNPAQEFFDKLKVGT
                     EKIQFDLLREFAAAKARLRNEATPRQARSKGLWRESAVDSAHKTSGSGNSLHLG"
     gene            complement(2107..>2332)
                     /locus_tag="VNY06_04610"
     CDS             complement(2107..>2332)
                     /locus_tag="VNY06_04610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012383145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DnaJ C-terminal domain-containing protein"
                     /protein_id="HWX14130.1"
                     /translation="GKQFKLKGKGMPVLRSRETGDLYIEVTVETPQSLTKRQRELLAE
                     FEALSSKDTHPESAGFFARMKDFFENLGGQ"
BASE COUNT          464 a          681 c          706 g          481 t
ORIGIN      
        1 tatcatattc tggccgaagc gtatgcgctg ccttctcgac gagagcggcg atcagattaa
       61 cgcctaggct catattgcct gagcgcacga tgacggcatg gcgcgcggcg gcgtcgagct
      121 ttgtgagatc gctcgggttc aagccggttg tgccgatcac atggacgatg cgcgcctgtg
      181 ctgcgagcgc cgcaagttcg accgttgatt gcgccgaagt gaaatcgagg attccatcgg
      241 cgaccgcaac ggctgtgagc gcatcgccgc ttatcaccac acccatttgg ccgcagccgg
      301 cgaggagccc cgcatcctgg ccgatcgcaa tggaatcggg ctgctcgatc gccgcagcga
      361 gatgcgcgcc gggcgtttgg cggataattt caacgaggac gcggcccatg cgtccagccg
      421 ctcccgcaac gacaaggcgc atatcgctca tgacggatgt ccccgattta tcctagcctt
      481 gtctataatc cctggaggcc gcaaggaaaa ctccagatta tgcgaatccg tcatcctgcc
      541 ttcatggctg ctttctatcc ttctttgttc gtggcgtgcg gaagaacgca ccatgatcgc
      601 gcgccgggcc ggaggtacgc ccggcgtcat ggttcttttt tggtcgccag cttccgctcc
      661 cgccggccgt atacccggca caacgcgcgc gcatgacgct tacctcatgg gcgcagcctc
      721 gcgcgcttgc gccgcacggc gggcgacgag gtcagccact ccataggcat aaccggcctc
      781 agcgaggtcc tcagcgttgt aggacgtcaa aacggttaca ccaaggagag gaagtcttcg
      841 agcgccccgc ggcaaatttc gcggcctgcc cggctcaccg tggctgcgat atcgtgcagc
      901 ttgagatcga gaaagacctt cttggcgctt tgaatcatcg cgggcgaggg agagcccgcc
      961 gccataggcg agttccaaac caattttgta aaagcgcgct ccattaccta gtatcggcac
     1021 caccgcctcg gcttcccgtt gcatctcgaa atcgagcgca atgatgagct tttttcgggc
     1081 ggcggcagtg tcgtgcgggg cggtggcagg gcggttcatc ccaaatggag ggaattaccg
     1141 ctgccagatg tcttgtgcgc gctgtccaca gccgattccc gccaaagtcc tttgctccgg
     1201 gcttgccggg gtgtcgcttc gttacgaagc ctggccttgg cggccgcaaa ttccctcaac
     1261 aaatcaaatt gaatcttttc ggttccgact ttgagcttgt cgaagaattc ctgcgctggg
     1321 ttcggccgct tgagctttag ctgcgtgtcg gcaccggtca tgcgccggta tatccaaaca
     1381 cgcgccggcg gcagatttag ccagactgga ggagaagcta cggcgtgaag agcgagcgcc
     1441 gacagtttgt cacgcgggat cggcgagaga gggctatagg taaattggac gaagcatcca
     1501 tccggggcca tgcagccact agcttcagca agaagcgcca gtctttggga ctcgggtttt
     1561 aagagcaagg gtaggcttga aacgatcgcg ggtggaggac ggcgcagtcg gccctgcagc
     1621 gttttagaca gctgataggc atcccccttg atgacgtgaa cgcccggaaa gcgccgcctg
     1681 agaaggcagc agaattctgg gtcatattcg ataagaaaga gtcgctgagg cgcaatgccg
     1741 cgctctaaaa gcgcctctgt gattgcgccg gtccctggtc caagctcgac gataggtcct
     1801 ttcttgcgag gatctacata gcgcgccatc atacgcgcga gaaagggtcc ggatggggag
     1861 acagctccgg cgattccagg gttgtcgagc catgacttga aaaagcgcgc ctcatcggcg
     1921 aaccgcagtt ccattggtgt ggcggatttt cgtttgcgcc gccggctgcg cgcggatggg
     1981 ctcggcaaaa gtcgtgtcct ccccgaaaga tcgcggcgga cgcttcgcac gccgtgtcgc
     2041 cgtcaatagc gagggtccag aaaacacaaa tctgtcaaac gagcaagtag ggcgtcttaa
     2101 gcgagcttat tggccgccta aattctcgaa gaaatccttc atcctggcaa agaagcccgc
     2161 agactccgga tgcgtgtctt ttgaagagag agcctcgaac tccgcaagaa gctcgcgttg
     2221 tcgcttggtg aggctttgcg gggtctccac tgtcacctcg atatagagat caccggtttc
     2281 gcgcgaccgc agcaccggca tgcctttgcc cttgagctta aactgtttgc cg
//
LOCUS       DATMPU010000199         2150 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_2955159,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000199 DATMPU010000000
VERSION     DATMPU010000199.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 2150)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2150)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2150
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2955159"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..331)
                     /locus_tag="VNY06_04615"
     CDS             complement(<1..331)
                     /locus_tag="VNY06_04615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012590732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4147 domain-containing protein"
                     /protein_id="HWX14131.1"
                     /translation="MERDPRQLLHAMFDAAVGAALPEKCLPPLLPPRPPGRVIVVGAG
                     KAGGSMAKAVEDNWEGSLEGLVVTRYGHGAPCQRIELVEASHPVPDAAGEEAARRILA
                     MVAGLTPE"
     gene            858..2045
                     /locus_tag="VNY06_04620"
     CDS             858..2045
                     /locus_tag="VNY06_04620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012590733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate--CoA ligase subunit beta"
                     /protein_id="HWX14132.1"
                     /translation="MDVHEYQAKEILANFGVAVPQGSVAYSPEQAVYCATELGGWHWA
                     VKAQIHSGGRGKAGGVKLCKTYHEVRAAAAELLGKTLVTNQTGPEGKVVQRVYIEVAE
                     PFEREIYLGFVLDRKVERVRVIASRFGGMDIEEIAKEHPEEILQVVVEPAVGLQPFEA
                     RELAFGLGLSLKQVSRAVATILGCYRAFRDLDATMVEVNPLVVTKDDRVLALDAKMSF
                     DDNALFRRGTVADMRDHSQEDPREVQAAEHNLNYVGLEGNIGCIVNGAGLAMATMDMI
                     KYAGGSPANFLDVGGGASPERVAAAFRLVLSDRNVRAILVNVFAGINRCDWVAEGVVK
                     ACRELKVDVPLVVRLSGTNVEAGREIIAKSGLPIISAETLADAAKAAVDAAQSAPERG
                     GKA"
BASE COUNT          440 a          611 c          679 g          420 t
ORIGIN      
        1 cttcgggggt taggccagca accatggcaa gaatgcgcct tgccgcctct tccccggctg
       61 catcagggac aggatggctc gcttccacca gttcgatgcg ttggcaaggc gcgccgtgcc
      121 cgtagcgggt aaccaccaac ccttcgagcg acccctccca attgtcctcg acggctttgg
      181 ccatcgagcc gcccgccttg cccgcgccca cgacgatcac gcggccgggc ggccggggcg
      241 ggagaagcgg cggcaagcac ttctcgggca atgcggcgcc gactgccgca tcgaacatgg
      301 cgtgaagcaa ctgacgggga tccctttcca tgattatgag ccgctttcct tcatggccac
      361 ccggagagct ttgagaggtt gtttccggaa gcgtagcgag cggccgggac tcggtgtgac
      421 taaagcgtcc gaggtttcga tcaaactgca gaagcgggca tagccgctcg tcaataaatg
      481 catatgaacg ccatgaaccc acgaacgaag ccgttacata aacgcaatag gtgagctttc
      541 aatcattttc tgaaccgact ctgcatcgca aggcatcaac caaacatatg gaaacaccaa
      601 gatttccaac atatgcgcgc gttataaaag ctttgaattc ccgccggagg ttcctcctat
      661 ggccgttttg gtgcgcttgt taagctgcgt cccaacgcca cagggagggt tgctaagact
      721 ggaatttgac aataagcgtt gcgctgggct catgggcgat gcaggccgca cgcatgagca
      781 gcaagagtgc acggctacgc agacgtgcgg gaattgatag ggccagcggt tgaaactgga
      841 ttgcaaggag gggcattttg gacgttcacg aatatcaagc caaggagatt ttggcgaatt
      901 ttggtgtggc ggttccgcaa ggatccgtcg cctatagtcc cgagcaggcg gtctattgtg
      961 cgaccgagct cggtggctgg cattgggcgg tgaaagcgca aattcactcc ggcggccgag
     1021 gcaaggcggg cggagtcaag ctctgcaaga cctatcacga ggtgcgcgct gcggcagccg
     1081 agcttctcgg caaaactctg gtcaccaatc agactggccc tgaaggcaaa gtcgtgcaac
     1141 gcgtctatat tgaggtcgcc gaacccttcg agcgcgagat ttacctcggc ttcgttctcg
     1201 accggaaggt cgagcgcgtg cgggtcatcg catcgcgctt tggcggcatg gatatcgagg
     1261 agattgccaa ggagcacccg gaagaaatcc tgcaagtcgt cgtcgagcct gccgtcgggc
     1321 tgcagccgtt cgaggcacgc gaactcgcct tcgggcttgg ccttagtctc aagcaggtga
     1381 gccgcgcggt cgccactatt cttggctgct accgggcgtt ccgcgatctt gacgcgacca
     1441 tggtcgaggt caatccgctg gtcgtaacca aggatgaccg cgtgctcgcc ctcgatgcca
     1501 agatgtcctt cgacgataat gcgctcttcc ggcgcgggac ggtcgcggac atgcgcgacc
     1561 attcgcagga ggatccgcgc gaagttcagg ctgccgagca caatctcaat tatgtcggcc
     1621 tggaaggcaa catcggctgt atcgtcaacg gcgcgggact cgccatggcg acgatggaca
     1681 tgatcaaata tgccggcgga agtccggcga attttctcga cgtaggcggc ggcgcctcgc
     1741 cggaacgggt ggccgctgca tttcgtctgg tgttgtccga ccgaaacgtg cgcgccatcc
     1801 tggttaatgt gtttgccggg atcaatcgct gcgactgggt ggccgaaggg gtcgtcaaag
     1861 cctgccgtga gcttaaggtc gatgtcccat tggtggtgcg cttatccggc accaatgtgg
     1921 aggctgggcg tgagatcatc gcgaaaagcg gccttccgat catctcggcc gaaaccctcg
     1981 ccgatgcagc gaaggcggca gttgacgcgg ctcaaagcgc ccccgagagg ggcggcaagg
     2041 cctgactgca catgatgaag gggtgcgcgg cagaggcgcc ggctgctgac cggaaaatta
     2101 gaacgacgcc gtgccttgtt ctttactccg atggcgaaaa ggtcttggac
//
LOCUS       DATMPU010000200         6787 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_2027563,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000200 DATMPU010000000
VERSION     DATMPU010000200.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 6787)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6787)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6787
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2027563"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..423)
                     /locus_tag="VNY06_04625"
     CDS             complement(<1..423)
                     /locus_tag="VNY06_04625"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009029715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HWX14133.1"
                     /translation="MGSPPYLRLGVNVDHVATVRNARGGAVPDPIRAALLAIEAGADG
                     ITAHLREDRRHIRDDDMARLKTEIAKPLNFEMAATDDMVAIALDTRPHACCLVPEKRS
                     ERTTEGGLDVIGGQEHLKIVTAELLKAGVRVSLFIEPSF"
     gene            complement(423..2624)
                     /locus_tag="VNY06_04630"
     CDS             complement(423..2624)
                     /locus_tag="VNY06_04630"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013166439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HWX14134.1"
                     /translation="MMRQYELVDRVRRYNPQADEDALNRAYVYAMKAHGAQKRASGDP
                     FFSHPLEVAAILTDMKLDDATIVAAVLHDTIEDTDSTREEIDKLFGSDIGRLVDGLTK
                     LKKLDLVSRRAEQAENFRKLLLAVVEDVRVLLVKLADRLHNMRTLQFVPHDKRARIAT
                     ETLDIYAPLAGRMGMQRMRDELEDLAFRHLTPDAYAMVAGRLADLRLKNGKIIAKIEK
                     ELGSELAARDIHAKVEGRQKQPYSVWRKMERKSISFEQLSDIFGFRVVVEDIEDCYRV
                     IGVVHTKWAVVPGRFKDYISTPKQNDYQSIHTTVVGPGRQRVELQVRTALMHDIARNG
                     IAAHALYKDGQVADKDTLTNESRAYQWLRRTVSLLSEGATPEEFLEHTKLELFHDQVF
                     CFTPNGRLIALPRGATPIDFAYAVHTDVGNSAVGAKINGRQAPLLSELQNGDEVEIAC
                     AEGQTPPAVWEGLVMTGKARAAIRRATRDAVRAQYAALGRQIVTRAFERAGKAFAEDK
                     LKIALPKLARPTTEDTLAAVGRGEMFSGDVVRAVYPEFLEDRKVIVPRPRGETGWLSM
                     KKATSLVFTLTGNETDKKANPIQDAQEHLPIRFAPNGGAVPGDRIVGILTAGEGITIY
                     PIQSPDLIAFDEEPERWLDVRWNFEECRHELFPARLRLTAINEPGTLGQIASVMGDHA
                     ANIDNIVIRPISADFRELTIDIEVIDLKHLNTIVSHLRARPVVSQVERVNG"
     gene            complement(2951..3334)
                     /gene="rpoZ"
                     /locus_tag="VNY06_04635"
     CDS             complement(2951..3334)
                     /gene="rpoZ"
                     /locus_tag="VNY06_04635"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012385408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HWX14135.1"
                     /translation="MARVTVEDCIDKVENRFELVLMASHRARVIAAGAPIMVSRNNDK
                     NPVIALREIAESSLVPEDLKEDFIQSLQKHVEVDEPEAEVVPALPSATEGADAVGEFD
                     RMTEEDLLRGLEGLVPPAETDEDGD"
     gene            3692..4306
                     /locus_tag="VNY06_04640"
     CDS             3692..4306
                     /locus_tag="VNY06_04640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020176759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HWX14136.1"
                     /translation="MAEQDRIALFIDGANLYATAKSLGFDIDYKRLLKEFQSHGKLIR
                     AFYYTALVEDQEYSSIRPLVDWLDYNGFAVVTKPTKEFVDALGRRKVKGNMDIELAVD
                     AMEMADHIDHIVLFSGDGDFRSLVEAVQRKGVRVSVVSTNVTQPAMVADELRRQADDF
                     IDLAQLAPKIGRDPAERIDRAQKIQERRMATADQFDPALVEDRP"
     gene            4354..5052
                     /locus_tag="VNY06_04645"
     CDS             4354..5052
                     /locus_tag="VNY06_04645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011510727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HWX14137.1"
                     /translation="MSAFQRCRADLEPNPDCSFCPRLSEFRNSLRAIQPDWHNAPVPS
                     FGASDARLLVVGLAPGLRGANRTGRPFTGDFAGHLLYETLIDFGFATGAYAESSSDSL
                     RLLSCLITNAVRCVPPQNKPAPAEIKNCRSFLRITIAALCQLKVVVALGRVAHDSILC
                     AFEVKPAAFPFAHGAEHVLGAEASSHSRRRRVKVVTIIDSYHCSRYNTNTGVLTRDMF
                     RMVFAKAREKLQCP"
     gene            5156..5599
                     /locus_tag="VNY06_04650"
     CDS             5156..5599
                     /locus_tag="VNY06_04650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX14138.1"
                     /translation="MVTQSAEQHPHSERVSEAAESANKASLAKIVILSLLAHICLVLA
                     LVRLDPLSFPASGSREIPVQVVVAEPPPPQSPAAARKETPVNERAPQETVEAQNGTNA
                     HGAASEGEGAKEGLPGAQPSLPSMQRRIVFVVLRYPLRRQTALKR"
     gene            5603..5839
                     /locus_tag="VNY06_04655"
     CDS             5603..5839
                     /locus_tag="VNY06_04655"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HWX14139.1"
                     /translation="MKQYPESAIRRGVKGTATIGFVLDESGGVALVSLLRSSGEADLD
                     SESVALVRRAAPFPPPPPGVKSSFAIELAFGNRK"
     gene            5823..6755
                     /locus_tag="VNY06_04660"
     CDS             5823..6755
                     /locus_tag="VNY06_04660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012653384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HWX14140.1"
                     /translation="MATENEHETLPSVRKQGQSRRNKGIMMGMKESSAGVWGLRRAVR
                     ALTSLGLAILMWQGCPGTSLKAAEPNPALKEVIEGQQRSAANIARDPYRHPLEVLTFF
                     GVEPQSNVVEIAPGKAGYWIEVLAPYLKDRGRYTASGDATRDITPLNARIAISPEIYG
                     KIAITEFAGGDQEIASLGSADFVLTFRNIHNWMRAGTAEAAFRAFYRALKPGEILGVE
                     EHRARPDQPQDPQAKSGYVRQDYAIALAEKAGFKLIGSSEINANPKDSKDYPEGVWTL
                     PPTYRLGAQDRKKYPVIGESDRFVLKFVKPAEAK"
BASE COUNT         1457 a         1914 c         1922 g         1494 t
ORIGIN      
        1 gaaagatggc tcgatgaaca aggagacgcg taccccggct ttaagcagtt cggcggtgac
       61 gattttcagg tgttcttggc cgcctatgac atcgaggccg ccctcggtgg tgcgctcgct
      121 gcgcttttcc gggacaagac aacaggcatg cggccgcgta tcgagggcga tcgccaccat
      181 gtcgtcggtt gccgccatct cgaaatttaa cggctttgca atctcggttt tgagccgcgc
      241 catgtcgtcg tcgcggatat ggcgccggtc ctcgcgcaaa tgggcggtga ttccgtcggc
      301 gcccgcctca atggcgagca aggccgcgcg gatgggatcg ggaaccgcgc cgccccgggc
      361 attgcggaca gtcgcgacat ggtcgacatt gacgccgaga cgcagatagg gaggcgatcc
      421 cattagccgt tgacccgttc gacttggctg acaaccgggc gcgcccgcaa atgcgagacg
      481 atcgtgttca aatgcttcaa gtcgatgacc tcgatatcga tggtgagttc ccggaaatcg
      541 gcggaaatgg gccgaatgac aatgttgtcg atgttggccg catgatcgcc catgacgctt
      601 gcgatttgtc ccaaagttcc gggctcgttg atcgcggtaa gccgcagccg ggcagggaag
      661 agttcatgcc ggcattcctc aaaattccag cgcacatcga gccagcgctc tggctcttcg
      721 tcaaatgcaa tgagatcggg agattggatt ggataaatgg tgattccttc gccagcggtc
      781 aaaatgccga cgatacgatc gcctggcacc gcgccgccgt taggggcaaa gcgaatgggc
      841 aaatgctctt gcgcatcttg aatggggttt gctttcttgt ccgtctcgtt gccggtgagc
      901 gtgaagacga ggctcgttgc tttcttcatg cttaaccagc ccgtttcgcc gcgcggacga
      961 ggcacaatca ccttgcggtc ttccaagaat tctggataga cggccctgac gacatcacca
     1021 gaaaacatct cgccgcgccc gaccgcggcg agcgtgtcct ccgtcgtcgg gcgcgcaagt
     1081 ttgggcaatg cgattttcaa tttgtcttcc gcaaaggctt ttccggcgcg ctcgaaggcg
     1141 cgggtgacga tctggcgtcc aagagccgca tattgcgcgc ggaccgcgtc gcgggtcgca
     1201 cgccttattg ccgcgcgggc cttgccggtc atgacgagac cttcccaaac ggctggcggc
     1261 gtctggcctt cggcacatgc gatctcgact tcatcgccat tttgcagttc ggaaaggagc
     1321 ggtgcctgac ggccattgat ctttgcaccg accgcggaat ttccaacgtc ggtgtgaacc
     1381 gcgtaagcaa agtcaatcgg cgttgcaccg cgaggcagcg caatcagccg tccgttcggt
     1441 gtgaagcaaa agacttggtc atgaaagagt tcgagcttgg tatgctccaa aaattcctca
     1501 ggcgtcgcgc cctcggagag gagcgacaca gtgcgacgca accattgata ggctcggctt
     1561 tcgttggtca gtgtgtcttt atctgcaact tgtccgtctt tatagagagc gtgagcggcg
     1621 atgccgttgc gggcaatatc atgcatgagt gccgtgcgta cttggagctc gacacgctgt
     1681 cggccgggcc ccaccacggt ggtatggatc gactggtagt cgttttgctt cggcgttgaa
     1741 atatagtcct tgaaacggcc tggaacgacc gcccatttcg tatgcacgac tccaatgaca
     1801 cggtagcagt cctcaatatc ttcgaccaca acgcggaagc caaaaatgtc tgacagctgc
     1861 tcgaaggaga tggatttccg ctccatcttc cgccacacgg aatagggctg tttttgccgc
     1921 ccttcaactt tggcgtggat atcccttgcc gcgagttcgg aaccgagctc cttttcgatc
     1981 ttggcgataa tcttcccgtt tttcaggcgc agatccgcga gccttcccgc gaccatcgca
     2041 taggcatctg gcgtcagatg acgaaaggcc aaatcctcga gttcgtcgcg catccgctgc
     2101 atgcccatcc ggccggcgag cggcgcatag atatcgagcg tttccgtggc gatccgcgcg
     2161 cgcttatcgt gcggcacaaa ctgcaaggtg cgcatattgt gcagccggtc ggcaagctta
     2221 acgagaagga cgcgaacatc ttccaccacg gcgagaagga gcttgcggaa attctccgcc
     2281 tgctcggcgc gccttgatac aagatcgagc ttcttgagtt ttgtcagccc gtcgacaaga
     2341 cggccaatgt cgctgccaaa aagcttgtca atttcctcgc gcgtggaatc ggtgtcttca
     2401 atcgtatcat gcaagacggc cgcgacgatt gtcgcgtcat cgagcttcat gtcggtgaga
     2461 atggccgcga cctcgagtgg atgagaaaag aaaggatcgc cggaagcgcg cttttgcgcg
     2521 ccatgcgcct tcatcgcata gacataggcg cggttgagcg cgtcttcgtc ggcctgcgga
     2581 ttatagcgcc gcacccggtc gacgagttca tattgccgca tcataattac gccataatta
     2641 cgccggcccg cagcacgact cgtcaaaaca tcctccgatg agaacgcaac cgagtcatgc
     2701 tccctttcgt ctaaaatttc accgcaggtt cagattcgaa aaatcttcat agcgcggata
     2761 cggctcatgc tccacgtaaa attgcgctct cgtgccagtg cgatcgcggt tcgccttgac
     2821 ctccgcgctc tttcgatttg caagcagcta tttgcgcaag accgaacggt aaaacctaag
     2881 cgcgcgccgt cgtagtcttg cgagcgataa gcgctatcgg agaagaaacc gctcgagcga
     2941 atgctgagtc ttagtcgccg tcctcgtccg tttcggcggg cggcacgaga ccctcgagcc
     3001 cgcgcaaaag gtcttcctcg gtcatccggt cgaactcgcc gacggcatcg gctccttcgg
     3061 tggctgatgg aagcgctggc acaacctcgg cctcgggctc gtccacttcg acgtgttttt
     3121 gcagggactg aataaaatct tccttgaggt cctcgggcac caaggacgat tcggcgatct
     3181 cacgcaaggc aatgactggg tttttgtcat tgttgcgact aaccatgatt ggtgccccgg
     3241 cagcgatcac gcgtgcgcgg tgactcgcca ttaagacgag ttcaaaccga ttctccacct
     3301 tatcgatgca atcttccacg gtgacgcgag ccatgccgtg cttactcctt gaagcagaag
     3361 cccttttgga tcaatttacg attatacttt tgcgtcgcac tgtggcaacc aaaaatatgc
     3421 cattcgccaa cttggctgct catataatct ttaatttcga caatgatggg cattcgcctt
     3481 aatattttca tcttgatgct cgagcctaga gtttagttgg tccttgacgc aaaggttaaa
     3541 taggaccaca taatatcgcc taaatctaac gcggcaactg accggtcgga ggaaagtgcg
     3601 cgcgggcgtc ggctagggag ctaatgttct tgctcccggt gacataaata atgaaaaaga
     3661 acatcacaag ttactattat gagatacgtt aatggctgag caggaccgca tcgcgctatt
     3721 tatcgacggc gcgaatctat acgcaacggc taagtcgctg ggatttgata tcgactacaa
     3781 gcgactgctc aaggaattcc agagccatgg aaaattgatc cgtgcgttct actatactgc
     3841 tttagtcgaa gaccaggaat actcatcaat acgcccgctc gtggactggc tcgattacaa
     3901 tggtttcgct gttgttacta agccaacgaa agaatttgtc gatgctctcg gccggcgcaa
     3961 agttaaaggc aatatggata tcgaacttgc ggtagatgcc atggaaatgg ccgatcacat
     4021 tgatcatatt gtcctatttt ctggagacgg cgatttccgc tcccttgtcg aagcggttca
     4081 gcgcaagggt gtgcgcgtgt ctgtggtttc aaccaatgtg acgcagcctg ccatggttgc
     4141 cgacgaattg cgccgtcagg ccgatgattt tatcgatctt gcgcaactcg cgccaaaaat
     4201 cggccgcgat cccgccgagc gaatcgaccg ggcccaaaag attcaggaaa ggcgcatggc
     4261 gacggcggat caatttgacc cggcgctcgt cgaagatcgc ccgtaataag gccggtcatt
     4321 tttaccagcg aaagaagggg cgggtaaaag accatgtcgg catttcagcg atgccgggca
     4381 gatcttgaac caaaccccga ctgctccttt tgcccacgtc tttcggaatt tcgcaattcc
     4441 ctcagagcaa tccagccgga ttggcacaac gctcctgttc catcattcgg agcgagtgac
     4501 gcccgcctcc ttgtcgtagg tctggcaccc ggcctgcggg gcgcaaatcg caccggccgg
     4561 cctttcactg gcgattttgc aggccatctc ctttatgaaa cgctgattga ttttggcttt
     4621 gccacgggcg cctatgcgga gtcgtccagc gacagcttgc gccttctgtc ctgcttgatt
     4681 accaacgcag tgcgctgcgt cccgccgcaa aacaagccag cgcccgccga aatcaagaat
     4741 tgccgttcct ttctaaggat aacgatcgcg gccttatgcc aacttaaggt ggttgtggcg
     4801 ctcggacggg ttgcccacga ctccatactt tgtgccttcg aagtaaagcc ggccgcgttc
     4861 ccattcgctc atggtgccga acatgtattg ggagcagagg cgtcttccca cagccggcgc
     4921 cggcgagtga aggttgttac tattatcgac agttatcact gttcacgata taacaccaat
     4981 accggagttc tgacccgaga catgttccgg atggtttttg ccaaggctcg ggaaaagctc
     5041 caatgccctt agttagtttt ggcgaaaccg gagccgccaa aggcctggcg gtgacagctc
     5101 aaggaggcaa tccttgccta tggacacaat atcatggcgc cgagggaggc ggcgcgtggt
     5161 tacccaaagt gccgagcagc atccccattc cgagcgcgtt tcggaagcgg cggaatcagc
     5221 caataaagct tcccttgcca agatcgtcat attatcgctt ctcgcccata tttgtctcgt
     5281 ccttgccttg gtgcgccttg atccactatc ttttccagcc agcgggtcgc gggaaattcc
     5341 cgtccaagtg gtggtcgcgg agccgccgcc tccacaaagt ccagccgcgg cgcgaaagga
     5401 aacgccggtc aatgagcgcg cacctcaaga aactgtggaa gcacagaacg gaacaaatgc
     5461 ccatggtgcc gccagcgagg gcgagggggc aaaggaaggt ctgcccggag cacagccctc
     5521 gctcccttcg atgcagcgcc gcatagtttt cgtagttttg cggtacccgt tacggcggca
     5581 gacggcactg aaacgataaa gcgtgaagca atatccggaa agcgccatta gacgcggtgt
     5641 gaaggggacc gccacgatcg gatttgtgct cgacgaatcc ggcggcgttg cattagtttc
     5701 cttgttgcgg tcgagcggcg aagccgatct tgattccgaa agcgtggccc tggttcgccg
     5761 cgctgcgccc ttcccgccgc cgcctcctgg cgtcaaaagt tcctttgcca tagagctcgc
     5821 ttttggcaac agaaaatgag cacgagactc ttccctcggt ccggaagcaa gggcagagcc
     5881 ggcgcaacaa agggattatg atggggatga aagagagcag tgcaggagtt tgggggttac
     5941 ggcgggcggt ccgggctttg acctccttgg ggctggccat tttgatgtgg cagggctgtc
     6001 caggcacatc gttgaaagct gccgaaccca accctgcgct taaagaagtc attgagggtc
     6061 agcaaaggag cgccgcgaat attgcgcgtg acccctaccg gcatcctttg gaagttttga
     6121 catttttcgg cgttgagcct cagtcgaatg tggtcgagat tgcgccggga aaagctggct
     6181 actggattga ggttcttgct ccctatctca aggatcgtgg ccgctatacc gcctctggag
     6241 acgccacgcg ggacattacg cctcttaatg ctcggattgc gataagcccc gagatctatg
     6301 gcaagatcgc gattaccgaa ttcgccggcg gcgatcagga gattgcatcg ctgggcagcg
     6361 ccgatttcgt gctcacattc cgaaacattc acaattggat gcgcgctggc accgcagagg
     6421 cggcattccg tgcgttttac cgggctctaa aaccaggcga aatcttaggt gtcgaggaac
     6481 atcgggccag acccgatcaa ccacaagatc cgcaggctaa gagcggctac gtgcgtcagg
     6541 actatgcaat tgcgctcgcc gaaaaggccg ggttcaaact tattggctcc tccgagatca
     6601 acgcaaatcc aaaagacagc aaggactatc ccgaaggtgt gtggaccttg cccccaactt
     6661 accggctggg ggcgcaggac cggaagaaat atcccgtgat cggcgaaagc gaccggttcg
     6721 tattgaaatt cgtgaagccg gccgaagcga aatagccgcc gcctgcctgc acggaccgcg
     6781 cccgcca
//
LOCUS       DATMPU010000201         4408 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_2029035,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000201 DATMPU010000000
VERSION     DATMPU010000201.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 4408)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4408)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4408
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2029035"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..310
                     /locus_tag="VNY06_04665"
     CDS             <1..310
                     /locus_tag="VNY06_04665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX14141.1"
                     /translation="ASRLHMLPVGQSDDLDLHELENIVDALAETYDFTLIISSPIDQV
                     DTAKYVAAKSDFVLLASPVSADGSVVAAEWQLIESGAAEILLIGPETDVGQSLARNAA
                     "
     gene            613..996
                     /locus_tag="VNY06_04670"
     CDS             613..996
                     /locus_tag="VNY06_04670"
                     /inference="COORDINATES: protein motif:HMM:NF018630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1289 domain-containing protein"
                     /protein_id="HWX14142.1"
                     /translation="MNLETQSVPSPCIGICTMDEASGLCRGCGRSADEIVEWSSAEDS
                     RRKAIWAALPDRIDALGILITRLPWGHGQIIILSQRVSINDQEHGSLDAMAQALNSCA
                     AMMSHATFPCRAIEFPRSRSAAGCS"
     gene            912..1538
                     /locus_tag="VNY06_04675"
     CDS             912..1538
                     /locus_tag="VNY06_04675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX14143.1"
                     /translation="MCRHDEPRDISVSGNRISAISERSGLQLTIDEKVRALRLRAGPK
                     QSDFRAIFLVVLKAKANLPVATALTPLGCDEGAIQPECRNEHQFDLGLGRADLRFCVR
                     TATSELRERLTHASGLAFTDLAQTAGSTVLTQSATRVVESPIGRVEIFPSVPPPREQS
                     PEGPRSFLSLEKLTSGRSTPPGIDLPPVYVLGATFYPHILGAGTDISS"
     gene            1563..2618
                     /locus_tag="VNY06_04680"
     CDS             1563..2618
                     /locus_tag="VNY06_04680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012383815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="COX15/CtaA family protein"
                     /protein_id="HWX14144.1"
                     /translation="MSKLMVHLSSVHTGAVAIWLWIVAALVFLMVIVGGATRLTESGL
                     SITEWKPLTGVIPPLSQAEWLAEFENYKKIPQYAELFPDMDLGGFKFIFFFEWAHRLL
                     GRLIGAVMGVPLIFFWARGFLPPGFKPKLLGILALGGLQGFVGWWMVKSGLSDRVEVA
                     QERLAIHLLLASLTFAALVWVASSLRKGAAEIAAPNVRGLKWFAAATVLLVLVQIGLG
                     GLVAGLRAGHAFNTWPLIDGYFWPPLNKLTVLAPLWRNFLDNILVVQFQHRMIAYALL
                     ALTLVQALYARRVVPKSRARRRATAIAALATAQIAIGIVTLVLVVPLWAGLLHQAFAM
                     IVLGMAVVHAQELSQAR"
     gene            complement(2635..3066)
                     /locus_tag="VNY06_04685"
     CDS             complement(2635..3066)
                     /locus_tag="VNY06_04685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018900233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HWX14145.1"
                     /translation="MSEETDIFLRDILSSVRTIAMVGASDRMSRPSFGVFAFLLSHGY
                     HVIGVNPNLAGKSVHGTIFSKSLSKVPGPIDLVDIFRNSQAAGEAVDEALALDPRPTV
                     IWMQIGVNNEEAASRARALGVKVVMDRCLKIEYQRLIIRKL"
     gene            3310..3780
                     /gene="rplM"
                     /locus_tag="VNY06_04690"
     CDS             3310..3780
                     /gene="rplM"
                     /locus_tag="VNY06_04690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018407796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HWX14146.1"
                     /translation="MFHKTYSSKAADIEKKWVLIDADGLVVGRLASLIALRLRGKHKP
                     TFTPHMDDGDNVIVINAEKIVLTGRKREQKVYHHFTGYPGGIKERSAKFILEGRFPER
                     IVEKAVERMLPRGPLGRMQLGNLRVYKGAEHPHAAQQPVTIDIASLNRKNARRD"
     gene            3783..4259
                     /gene="rpsI"
                     /locus_tag="VNY06_04695"
                     /pseudo
     CDS             3783..4259
                     /gene="rpsI"
                     /locus_tag="VNY06_04695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012384376.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
BASE COUNT          956 a         1186 c         1232 g         1034 t
ORIGIN      
        1 agcttcgcgg cttcatatgc tccctgttgg acaaagcgat gatcttgatc ttcacgaatt
       61 ggaaaacata gtcgatgctc tggccgaaac ttatgatttt accctgatca tatcatcgcc
      121 tatagaccag gttgatacgg ccaaatatgt ggccgcgaaa tccgatttcg tgctgctggc
      181 aagtcccgta agtgccgatg gttcagttgt cgcggcagag tggcaattga tcgaaagcgg
      241 cgcagccgag attttgctca ttggccctga aacggatgtt gggcaaagcc tcgcccgcaa
      301 cgcagcttga caaaaaacgc cgcaagcgcc caaactcaag aattaggttt ttccgtccat
      361 tgaacgagcg gcacgatcca gccgatcctt agacttgtga gtttcgcaag cactattgcc
      421 agcttgcaag tccggcaata aacaagcggg acgcttgcac gaataagctt gcgcatcggc
      481 cgcgcatttg cccaccatga attgtgtcct gcgcagcgca tggacttccg ccgcgctggc
      541 cgctatggct ttcgccctgc cgcaggcaaa gcgagcagaa acgtctggcg ccttctaatc
      601 ctgatacatc gaatgaatct cgaaacacag tcggtaccaa gtccttgtat cgggatatgc
      661 accatggacg aagcctcagg cttatgtcgc ggttgcggcc gttcggccga tgagatcgtc
      721 gaatggtctt cggccgaaga ctcgcgccgg aaggccatct gggccgcgtt gccagaccgt
      781 atcgacgctt tgggcatttt gattactcgt ttgccttggg ggcatggcca gattatcatt
      841 ttgtcgcaga gagtttctat caacgatcag gaacatgggt cattggatgc catggcgcag
      901 gcgctgaatt catgtgccgc catgatgagc cacgcgacat ttccgtgtcg ggcaatagaa
      961 tttccgcgat ctcggagcgc agcgggctgc agctgacgat tgatgaaaaa gtcagagcgc
     1021 tgcgattgcg cgctggcccc aagcagagcg actttcgcgc catttttctc gtcgtgttaa
     1081 aagcaaaagc aaatctgccg gttgcgaccg cattgacgcc gcttggctgc gatgagggtg
     1141 ccatccagcc agaatgccga aacgagcatc agtttgatct gggtttaggg cgtgcggatc
     1201 tccgattctg tgtgagaacg gcgacaagcg agttgcggga gaggctcact cacgcatctg
     1261 gcttggcgtt tactgatctc gcgcaaaccg ctggatccac ggtcctaact cagagtgcaa
     1321 caagagttgt tgagtcaccc ataggccgcg tggaaatatt tccttcggtt cccccgcccc
     1381 gggagcaatc gcctgagggg cctcgcagct ttttgtctct tgaaaaattg acgagcggcc
     1441 gttccacgcc gccaggcatt gatctgccgc ctgtctacgt tctgggtgca accttctatc
     1501 cccacatcct cggcgcgggg actgatattt cgtcatgatg ccactgctac aggaaaacga
     1561 tcatgagtaa gctgatggtc catctatcct ccgtgcacac gggcgccgtt gcgatttggc
     1621 tttggattgt cgcagctctt gtatttttga tggtgattgt aggcggcgca acgaggctca
     1681 ccgaatccgg tctttccatc accgaatgga aacctttgac gggcgtcatt ccaccactaa
     1741 gccaggccga gtggctcgcc gaatttgaaa attataaaaa gattccacaa tatgccgaac
     1801 tctttccgga tatggatttg ggcggcttca aattcatatt cttctttgaa tgggcccacc
     1861 ggctcttggg ccgtctgatc ggcgccgtca tgggggtgcc tttgattttt ttctgggcga
     1921 gggggttttt gcctcccggc ttcaagccta agcttcttgg aatattggcg ctcggagggt
     1981 tgcagggatt tgtcggctgg tggatggtca aatccgggct ctcggatcgc gttgaagtcg
     2041 cgcaggaacg gctcgctatc catctgctgc tggcttcgct gacatttgcc gcgctggttt
     2101 gggtagcaag ttcgctaaga aagggcgcgg ccgagatcgc ggcgccaaac gttcgaggac
     2161 taaaatggtt tgcagcggcc acggtgctgc tcgtattggt ccaaattggg cttggcggcc
     2221 tcgtcgcggg tctgcgcgca ggacacgcgt tcaacacttg gccactcatt gatggatatt
     2281 tttggccacc tctaaataaa ttgactgtcc tcgcaccgct ttggcgaaat ttcctcgaca
     2341 atattttggt ggtccaattc cagcacagga tgattgccta tgcgcttttg gcgcttacgc
     2401 tggttcaggc gctttacgca aggcgcgtgg tgccaaaaag ccgggctcgg aggcgtgcca
     2461 ccgcgatagc ggcgcttgcc acagcgcaaa ttgcaatcgg catcgtgact ctggttctcg
     2521 tggttcccct ttgggcaggg ctactgcacc aggcctttgc gatgattgtt ttgggaatgg
     2581 cggtggtaca tgcgcaggag ctttcgcaag cgcgttaggg aaggctaagg cgccctacaa
     2641 tttgcggatt atcaaacgct gatattcgat tttgaggcaa cggtccatga cgacttttac
     2701 cccgagcgct cgcgctcggg aagcagcctc ctcattgttg actccgatct gcatccaaat
     2761 gactgttggt cttgggtcga gtgcaagtgc ctcatcaacg gcttctcccg ccgcttgcga
     2821 attgcggaaa atatccacca aatcaattgg ccccggaact tttgaaagtg acttggaaaa
     2881 tatggtgccg tggacgctct ttccggcaag gtttggattg accccaatca catgatagcc
     2941 atgggatagg agaaacgcga aaacaccaaa ggaaggccgg ctcattctat cggacgcgcc
     3001 caccatcgca atggttctga cgctcgagag aatgtcacgc aaaaaaatat cggtttcctc
     3061 gctcaccgtt tttgtccatg ctgaggctgg gcttttgtag caaacgcggc gcgttctttt
     3121 atcgtgggtt gcgcattttg cgaaacaatt acggtaacat aataccgtac gatgtaacat
     3181 actgttttgc caaggccttc taatccgact tcaggcttga cccaaaaccc ttcggtgcat
     3241 aaaagaagcg cccatgaact tataggcccc gtcgatatgg ggtttgcgcg aaagcgatgc
     3301 ggaatccaaa tgtttcacaa gacttattcc tccaaggcag cggatattga aaaaaaatgg
     3361 gtgttgattg acgccgacgg gcttgttgtc gggcggctcg cgtctttaat tgcattgcgg
     3421 ctgcgcggca agcacaagcc tacgttcacc ccgcatatgg acgatggtga caatgtcatc
     3481 gtcatcaatg ccgaaaagat tgtgctgacc ggccgcaagc gcgaacaaaa agtctatcat
     3541 cattttacgg gctatcccgg cggcatcaag gaacgttcgg ccaagtttat tttggaaggc
     3601 cgatttccgg agcgcattgt cgaaaaagcc gtggagcgca tgctcccgcg cggcccgctc
     3661 gggcgcatgc agctcggcaa tttgcgcgtc tacaaagggg ctgagcatcc tcatgctgca
     3721 cagcagccag taacaatcga tattgcgagc ctcaaccgca agaacgcaag gcgtgactga
     3781 tcatggctga gatcctatct tctcttcagg acttaaaggg cgcaagcact cccgttccag
     3841 aggcggcccc gcattatgtg caaaagctcg acgcgcaggg acgtgcctat gccaccggca
     3901 agcgcaagga tgcggttgcc cgtgtttgga tcaagccggg cccgggaatg gccgtagtca
     3961 atggcaggcc gctcgacaca tatttcgctc gtcccgtcct cagaatgatc ctgcggcaac
     4021 cgcttgtcgc cgcaaagcga gccggtcaat atgatttaat gatcagcgtg gcaggcggcg
     4081 gactctcggg tcaagcgggt gccgtgcgcc acggcattgc caaggcgctt gccaatttcg
     4141 agccggatct gcggcctgtc ctcaagaaag aagggttttt gactcgcgac ccgcgcgtgg
     4201 tcgagcgcaa gaaatacggc aagaaaaaag cccgccggag cttccaattc tcgaaacgct
     4261 gcgccgccgc ccggcgattt cataacgtca aagcggctac tgttggccgc cactttgttt
     4321 tgacggcccg tagtgcgcaa acagacagat cgcggcaacg agcgactcga agcggtcacg
     4381 ctcttaattc agtatgacgt catcgcgg
//
LOCUS       DATMPU010000202         4881 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_2959966,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000202 DATMPU010000000
VERSION     DATMPU010000202.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 4881)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4881)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4881
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2959966"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..958)
                     /locus_tag="VNY06_04700"
     CDS             complement(<1..958)
                     /locus_tag="VNY06_04700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016919485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator"
                     /protein_id="HWX14147.1"
                     /translation="MFDRGAPGGTVSSSSTANSSAQKESGEKIGSGQAQVALILPMTQ
                     ASGPSNVGISLRNAAELGYLEAGNNDITLIVKDDRSTPEGARAAAQSAISEGAEIIIG
                     PLFAANAREVAAIARGVGKPIIAFSTDTSNAGPELFLLSFLIENYVDRVVDYAASKGK
                     KSMAALIPDNDYGRVAEGEFQQAAARNSIRVMTIEHYQAQTMASAVQRVAALNDQIDS
                     LFIPEQADAMQAMSQALVAAGINGRYQILGTGLWNDARVLKLPALQGAWFSAPENGGF
                     KAFAARYEAKYHSDPPRIATLAYDAVSLAAALAHTQGSQRYSA"
     gene            1336..2277
                     /gene="rsmI"
                     /locus_tag="VNY06_04705"
     CDS             1336..2277
                     /gene="rsmI"
                     /locus_tag="VNY06_04705"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003612004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HWX14148.1"
                     /translation="MSSGRVDGFEPNILRKDEKPPGVFTAFGLKAEAEPIRPGLHVVA
                     TPIGNLGDISFRALATLAAADAIIAEDTRVTKKLLAHYGIATPLIAYHEHNATVMRPH
                     LLARLADGASLALVSDAGTPLISDPGFKLVSDALAQNIEVVSVPGASAVLAALVVAGL
                     PTDRFFFEGFLPPKSAARRGRIAELAAIPGTLVFFESPRRVAETLGDLAAVLGSREAA
                     IARELTKHFETVRRRLLPDLAALVAAEPPPKGEIVVLVGPPGLAPPLAQEDVDARLGK
                     ALESLSVKDAAAVVSVETGQPRRKVYARAIELFARGR"
     gene            2274..2651
                     /locus_tag="VNY06_04710"
     CDS             2274..2651
                     /locus_tag="VNY06_04710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009763611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HWX14149.1"
                     /translation="MTAKGSRDRRRAHGLGLLAEHVAEVLLRLKGYRILTRRYSIREG
                     EIDLVARRGDTIAFVEVKVRPTLDEARTAIDFVKRRRVSRAARSWLSNPWAAPLTWRG
                     DAIFVAPWRFPRHQIAVIELDIG"
     gene            2836..3837
                     /locus_tag="VNY06_04715"
     CDS             2836..3837
                     /locus_tag="VNY06_04715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012589343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX14150.1"
                     /translation="MNPFPQGGIAEADTLTAPVAARDAIAADLIAVLVAVTAGDPRVL
                     TLKDRSALPSGPFELAHRSLQAGLRAWVERQTRLPLGYVEQLYTFADRDRAGTGQHVI
                     SISYLGLTREQPASGEYDASWRSWYSFFPWEDHRAGTPPIIEAALLPRLEAWANAAES
                     PGLSQDRKQRVGITFGGGAHKWNEELVLQRYELLFEAGLIPEAMRGTLAGSTRPSLKA
                     EASAGTGQPMILDHRRILATGIARLRAKIKYRPVVFELMPETFTLLQLQRCVEALAGL
                     LVHKQNFRRLIEQQELVEETGETTPDTGGRPAKLFRFRREILTQRAISGTKLPVSRA"
     gene            3897..>4881
                     /gene="nadA"
                     /locus_tag="VNY06_04720"
     CDS             3897..>4881
                     /gene="nadA"
                     /locus_tag="VNY06_04720"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010973561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HWX14151.1"
                     /translation="MATPFRETVRAEVPQAESLYAKVRHAIPAIEWPVHAPDIDAIMT
                     LKRARNAVVLAHNYQTPEIFHCIADFVGDSLALARQAMDTDADVIVLAGVHFMAETAK
                     LLNPDKTVLIPDLGAGCSLAESITPEDVLFLRKTYPGVPIVTYVNTSAAVKALSDICC
                     TSGNARGVIESLGVDRVIMLPDEYLARNVAAETKVEVIAWSGHCEVHERFSAEEVRQL
                     REDYPGVIVLAHPECPPEVVAEADFSGSTAAMSSYVSKNRPPRLVLLTECSMSDNVAI
                     QNPDLEFIRPCNLCPHMKTITLRKIRRALETMQYKVEIDPAIAIPTRRAVER"
BASE COUNT          978 a         1485 c         1414 g         1004 t
ORIGIN      
        1 tcgccgaata gcgttgggag ccttgtgtat gggcgagcgc cgcggcaagc gaaacagcgt
       61 cataggcaag ggtggcaatg cgcggcggat cggagtgata ttttgcctcg tagcgcgccg
      121 cgaacgcctt aaagccgccg ttctcaggag ccgagaacca cgcaccttgc aatgcgggaa
      181 gttttaagac ccgcgcgtcg ttccaaagac ccgtgccaag aatctggtat cttccattga
      241 tcccggctgc aaccagcgcc tgcgacatag cctgcatcgc atcggcttgc tccggtataa
      301 aaagcgaatc gatctgatcg ttcagcgcgg ctaccctttg cacagcgcta gccatggttt
      361 gcgcctgata atgctcgatg gtcatgaccc tgatgctatt gcgcgcagct gcctgctgaa
      421 actcaccttc cgcgacgcgg ccgtagtcat tgtccggtat gagcgcagcc atggactttt
      481 tgcccttgga agcggcatag tcaacgaccc gatcgacata attctcaatc aggaaggaaa
      541 gcagaaaaag ctcgggtccg gcattcgacg tatcggtcga aaatgcaata atcggctttc
      601 cgacgccgcg cgcgatggca gccacctcgc gcgcattcgc cgcgaacagc ggcccaatga
      661 tgatttcggc gccttccgaa atggccgact gcgcggccgc gcgcgcgccc tcgggggttg
      721 aacgatcgtc tttcacaatc aaggtgatgt cgttgttgcc ggcctcgaga tagccaagtt
      781 cagcggcgtt tcgcagcgat atgccaacat tgctcgggcc cgaagcctgc gtcatgggga
      841 ggatgagcgc aacctgggcc tggccggaac caattttttc gccgctctcc ttctgagcgg
      901 aactatttgc tgtcgaactc gacgaaaccg tcccgcccgg cgcgccacgg tcaaacatcc
      961 ctgtcacgga gttgcaagcc gtgagcgtca tgcaaagcga taagataccc aatgctcgca
     1021 gtgcgtcgct gcaacgcata acgcgcggcc agggcgtttt gtgcggcctg tgccgctcgc
     1081 attcgaaagg aatcattcgg ccgagttccg cattcgcatt ttcccgcacc tttcgtctgg
     1141 caagccgatg cagatagcca gaattgcatc gaaatcttgg tcattttcag tcataaattt
     1201 tcgccatcgg caatagagac agcagctagc gtcgggtagt tgcgaaaaag ccgcaggtgt
     1261 tctataaaac gaacaatctg gcgatttata gaacgaccct aaaaatagcc gggcattgcc
     1321 ggcgggctgg aaatcatgag ttcaggaaga gtggacggat ttgagcccaa catcctccgt
     1381 aaggatgaaa agccaccagg cgtatttacc gccttcggcc taaaggctga ggctgagcct
     1441 atccggcccg gtctccatgt cgtcgccacg ccgatcggca atctcgggga catttccttc
     1501 cgggccttag cgacacttgc cgccgccgac gcgatcattg ccgaggacac gcgcgttacc
     1561 aagaaattgc tcgcgcatta tggcatcgcg accccgctca tcgcctatca tgagcacaac
     1621 gcaacggtca tgcggccgca tctgctcgct cggctcgcag atggcgcgtc gctcgctctc
     1681 gtctcggatg ctggcacgcc attgatttcc gatcccggct tcaaacttgt cagcgatgcg
     1741 cttgcccaaa atatcgaggt ggtttcggtt ccgggcgcct cggccgtgct cgccgccctg
     1801 gtggtcgcgg gcctgccgac cgatcgtttc ttttttgaag gttttttgcc gccgaaaagc
     1861 gccgcaaggc gcggccgcat cgccgagctt gccgccatac ccggaacttt ggtgttcttc
     1921 gagtctcccc gccgcgtcgc cgaaactttg ggcgatttgg ccgctgtgct cggttcgcgc
     1981 gaggctgcga ttgcccgcga actcaccaag cattttgaaa ccgtccggcg ccggcttctg
     2041 ccggatctcg cggcccttgt cgcggccgag ccgccgccca agggcgaaat cgtcgtcctc
     2101 gtcgggccgc cgggacttgc cccgccgctc gcccaagagg atgtcgatgc caggctcggc
     2161 aaggcattgg agagtctctc ggtcaaggac gccgccgccg tggtgtcagt cgagactggc
     2221 cagccgcgta gaaaggttta tgcgcgggcg atcgaacttt tcgcgcgcgg gcgatgaccg
     2281 cgaaaggctc gcgtgatcgc aggagagcgc atggcctcgg gctccttgcc gaacatgtcg
     2341 ccgaagttct cttgcggcta aaggggtatc ggatcctcac gcgccgttat tccattaggg
     2401 aaggcgaaat cgatcttgtt gccaggcgcg gcgacacaat tgcctttgtc gaagtgaaag
     2461 tccggccgac tctcgatgag gcgcggaccg cgatcgattt cgtcaagcgg cgccgcgtct
     2521 cccgcgcggc cagaagctgg ctttcaaatc cctgggcggc gcctctgacc tggcggggag
     2581 acgccatttt tgtcgcgccc tggcgttttc cgcgacacca gatcgcggtg atcgagctgg
     2641 acattggata agcgcgcgaa atcctcatta atacgtgcgt ccattagcca tcggggtgtg
     2701 ccttcaagga cagcagttga aacttttcca agagactaga actttacgtt gaaacaataa
     2761 gatagagttc caataaggat ttcctcagcc gccagactga ggtattttcg ccacgttctc
     2821 ccttagtacc ggttaatgaa cccatttccc cagggcggta ttgccgaggc agatacgcta
     2881 accgcgccgg tcgccgcgcg ggacgcgatc gcggccgacc tcatcgcggt gctggtcgcc
     2941 gtgaccgcgg gcgatccgcg cgtcctgacc ttaaaggacc gttcggccct tccctcaggt
     3001 ccctttgagc tcgcgcaccg ctcgctgcag gctgggctgc gggcgtgggt cgaaaggcag
     3061 acaaggcttc cgctgggcta tgtcgaacag ctctatactt ttgccgaccg cgaccgcgcc
     3121 ggcacaggac agcatgtgat ttcaatcagc tatctcgggc tcacccgcga gcagcctgca
     3181 tccggagagt acgacgccag ttggcgcagc tggtactctt tttttccgtg ggaagaccat
     3241 cgcgccggca ccccgccaat cattgaagcc gcacttttgc cgcgtctcga ggcctgggca
     3301 aatgcagcgg aatctccggg cctaagccaa gaccgcaaac aacgcgttgg gatcacgttt
     3361 ggcggcggcg cccataagtg gaacgaggaa cttgtcttgc aacgctacga gctgctcttc
     3421 gaagcgggtc tcatcccgga agcgatgcgc gggactctcg cgggcagcac gcgcccgagc
     3481 ctcaaggccg aagcctcagc cggaacaggc cagcctatga tcctcgacca ccgccgtatc
     3541 cttgcaaccg gcatcgccag gctcagagcg aaaattaaat accgcccagt tgtgttcgaa
     3601 ttgatgccag agacttttac gctgctgcaa cttcagcgct gcgtcgaggc gctggccggc
     3661 ctgctcgtgc ataagcagaa tttccgccgc ctgatcgaac agcaggaact cgtcgaggaa
     3721 accggggaaa ccacccccga caccggggga cgtcccgcaa aactgttccg gttcagacgt
     3781 gagatattaa cccagcgcgc aatctccggg accaaactgc ctgtctcaag ggcttgacaa
     3841 atcttatact cgacaccagc atatatgtca ctaatgctca ttcggagtat aaaaccatgg
     3901 cgacgccgtt tcgagaaact gttcgcgccg aggttccaca ggctgaaagc ctttatgcga
     3961 aggtgcgtca tgcgatcccg gcgattgaat ggccggtgca cgcgccggac attgatgcca
     4021 tcatgacgct caagcgggca cggaacgccg tcgttcttgc gcataattac cagacgccgg
     4081 aaatttttca ctgcatcgcg gatttcgttg gagacagcct cgcgctcgcg cggcaagcga
     4141 tggacaccga tgcggacgtc atcgtgcttg cgggcgttca tttcatggcc gagaccgcga
     4201 agctcctgaa tccggacaag accgttctca tcccggatct cggtgctggc tgctcccttg
     4261 ccgagtcgat cacgcccgaa gatgtgttgt ttctgcgcaa gacctatcct ggcgttccca
     4321 tcgtgacata tgtgaacact tccgcagcgg tgaaagcctt gtcggatatc tgctgcacgt
     4381 ccggcaacgc gaggggggtg atcgagtccc ttggcgttga ccgtgtcatc atgctccccg
     4441 acgaatattt ggcccgcaat gtcgcggccg agacgaaggt agaggtcatc gcctggtcgg
     4501 gacattgcga ggtccatgaa cgctttagcg cggaggaagt gcggcaactc cgcgaagatt
     4561 atccaggcgt gatcgtcctc gcccatccgg aatgtccgcc ggaggtcgtc gccgaggctg
     4621 atttttccgg ctcgacggcc gccatgtcgt cttatgtcag caagaaccgg ccgccgcggc
     4681 tggtgctttt gaccgaatgc tcgatgagcg acaatgtcgc gattcaaaat ccggacctcg
     4741 aatttatccg gccctgcaat ctctgcccgc atatgaagac gatcacgctc cgtaaaatcc
     4801 ggcgggcatt ggagacaatg cagtacaagg ttgaaattga tcccgctatc gcgataccga
     4861 cgcgccgcgc cgttgagcgc a
//
LOCUS       DATMPU010000203         2423 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_3875482,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000203 DATMPU010000000
VERSION     DATMPU010000203.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 2423)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2423)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2423
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3875482"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2423)
                     /gene="treS"
                     /locus_tag="VNY06_04725"
     CDS             complement(<1..>2423)
                     /gene="treS"
                     /locus_tag="VNY06_04725"
                     /EC_number="5.4.99.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012318581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047471 - maltose
                     alpha-D-glucosyltransferase activity [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose alpha-D-glucosyltransferase"
                     /protein_id="HWX14152.1"
                     /translation="YPSPQRDDGYDISYYRGINRAYGSLRDFKRFVREAHERGLRIIT
                     ELVINHTSDQHPWFQRARAAKPGSSARSFYVWSDTDHAYKDAPVIFLDTEKSNWTWDE
                     QAKAFYWHRFYAHQPDLNFDNSRVLQALLGVMDYWLDMGVDGLRLDAVPYLVEREGTD
                     CENLPETHAVIKKIRAAVDAKYPDRMLLAEANLWPEETAQYFGDGDECHMAFHFPLMP
                     RIYMALAQEDRHPITDIMRQTPEIPDGAQWAIFLRNHDEVTLSMVTSKERDYLWSFYA
                     ADPRARINLGIRRRLAPLLENDRRKIELLTSLLLSLPGTPILYYGDEIGMGDNIYLGD
                     RDSVRTPMQWSLDRNGGFSRADPAKLFLPAIQDPVYGFGAVNVEAQLASPSSLLTWMR
                     RMIAVRRSHLSFGRGTLRFLHPSNRKVLPYVREFEGERILCVANVSRAPQAVELDLTD
                     FKGSVPVELTAGSRFPIIDSRSYLLTLPAYGFFWFRMEAAEAGKESQEQQPAPGLFTL
                     VATGKIETILSGRELIAFERTVAPRFLASRYWFGHEGEPLVRVSVNDFGVLRHGAYCR
                     LVLPILDVEFAAGRKESYFTPLAAEVEREDGPSLVHAAARLRRGALTGLLYEAEACEG
                     FAHATLAALRRGETIVTGHGGKILFLPSPSLATETLTEDATVRRIGRGRRNFSLVVAN
                     QLMLKVHRRLEIGEDPEVEVLRFLTEVAHFANTPPLLGVVEHIDRSGNRAVLAILQTY
                     VRNQGDARAWMLGAMKRSLEAAALTFGKDGPARHEEFAAYEPHMRSLGISTAWMHMAL
                     ATPCADPAF"
BASE COUNT          558 a          705 c          689 g          471 t
ORIGIN      
        1 tggaacgcag gatccgcgca cggtgtcgcg agcgccatgt gcatccaagc tgtagagatg
       61 ccgagactcc gcatatgagg ctcatatgcc gcaaattcct catggcgcgc ggggccgtct
      121 ttcccaaagg tcaaggcagc cgcctctagg ctgcgtttca tcgcgccaag catccaagcc
      181 cgggcgtcac cctggttacg gacataagtt tgcaggatcg cgagcaccgc gcggttcccc
      241 gaccgatcga tgtgctcgac aacgccgagc aggggcggag tgttggcgaa atgagcgacc
      301 tcggtcaaaa aacgcaagac ctcaacttct ggatcttcgc caatttccaa ccgacggtgg
      361 actttcaaca ttaattggtt cgcaacaacg agcgagaagt tacgccgtcc tcgcccaatc
      421 cgccggacag tggcatcctc ggtcaaggtc tcagtagcca ggctaggcga tggcaagaac
      481 aaaatttttc cgccatggcc agtcactatt gtttcgccgc ggcggagcgc tgccaaggtc
      541 gcgtgagcaa agccttcgca tgcctccgct tcgtaaagca ggccggtcag cgcaccacgc
      601 cgtaatcgcg ccgcggcgtg cacaagggat ggcccatctt cccgttcgac ttccgcagca
      661 agcggggtga aataactttc ctttcggccg gctgcgaatt ccacatcaag aatcggaaga
      721 acgagacggc agtatgcacc atggcgcagc accccaaaat cattgaccga aacgcgaaca
      781 agcggctccc cttcgtgtcc aaaccagtac cgcgaagcca ggaagcgcgg cgccaccgtg
      841 cgctcgaagg cgatgagttc gcgccctgaa agaatcgttt cgatcttgcc ggtggcaacg
      901 agtgtaaaga gtcctggcgc cggctgctgt tcctggctct ccttgcctgc ctccgccgcc
      961 tccattctga accaaaagaa accgtaagcc ggtaacgtca aaagatagga ccgcgagtca
     1021 attatcggaa agcggctgcc ggcggtaagt tcgacaggca cactaccctt gaaatcggtg
     1081 aggtcgagtt cgaccgcttg aggcgcgcgc gagacatttg ccacgcaaag aatccgctcg
     1141 ccttcgaatt cgcggacata gggcagcact ttgcggttgg aggggtggag aaaccgcaac
     1201 gtgccacgcc caaacgaaag atgcgaacgg cggaccgcaa tcattcgccg catccacgtc
     1261 aatagactgg acgggcttgc gagctgggct tcgacattga cggcgccgaa tccataaacc
     1321 ggatcctgga ttgcgggcag gaagagcttg gctggatcgg cacggctgaa gccgccattg
     1381 cggtcaagcg accattgcat cggcgtccgg acactgtccc tgtcgccaag atagatattg
     1441 tcgcccatgc cgatctcgtc cccataataa agaattgggg tcccgggcag cgataacagg
     1501 agcgaagtca aaagctcgat cttgcgacgg tcattttcga gcaagggagc gagccggcgg
     1561 cggatgccga gattgattcg cgcccgggga tcggcagcat agaaagacca cagatagtcg
     1621 cgctccttgc ttgttaccat cgacagagtc acctcgtcat ggttgcgcag aaagattgcc
     1681 cattgcgcgc cgtccgggat ttcaggggtc tgcctcatga tgtcggttat cggatgacga
     1741 tcctcctgcg ccaaagccat gtagatgcgt ggcatgagcg gaaaatgaaa tgccatatgg
     1801 cattcatcgc cgtcaccgaa atattgggcc gtctcctctg gccaaagatt ggcttccgcg
     1861 agcagcatcc ggtccggata ttttgcgtcg acggcggctc ttatcttctt gatgaccgcg
     1921 tgagtctcgg gcaagttttc acaatcggtc ccttcccgct caacgagata gggaactgca
     1981 tcgagccgca atccgtcaac acccatgtcg agccaatagt ccatcacgcc aagcagggcc
     2041 tgcaggacgc gggaattatc gaaattcaag tcgggctgat gcgcgtaaaa gcgatgccaa
     2101 taaaatgctt ttgcctgctc atcccaggtc caattcgact tttcggtatc aagaaagata
     2161 actggggcgt ccttgtaggc gtgatcagta tccgaccaaa cataaaagct ccttgccgac
     2221 gagccaggct tcgccgcgcg cgcacgctga aaccagggat gctgatccga cgtatggttg
     2281 atcaccagtt ccgtgatgat gcgaagtcca cgttcgtgcg cctcgcgcac gaaacgtttg
     2341 aaatcgcgca ggctcccata ggcacggttg atgccgcgat aataggaaat gtcatagccg
     2401 tcatcgcgct ggggagacgg ata
//
LOCUS       DATMPU010000204         3299 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_784641,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000204 DATMPU010000000
VERSION     DATMPU010000204.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 3299)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3299)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3299
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_784641"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(145..954)
                     /locus_tag="VNY06_04730"
     CDS             complement(145..954)
                     /locus_tag="VNY06_04730"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HWX14153.1"
                     /translation="MSMLKNDTQADVVVHENREFSPLGPESGSDIGGLRLAVPFSAFV
                     VTCALFGYAIAAGSRGDDTPIVSESTAAKSSSFAPEAGWMKNLTARLSVFGYESSYTM
                     LPGSRRSFDFVPPGVPRFVAAAMTDSGLLMRLAGPVPAAGQAALNSLTIEFPGNSARI
                     PASNFGVITKVAEVIKTLPSETVVEVIGYTAGSWHSAQGTALSRKRANSVYRALVHAG
                     ISPAKLRPMGYGSASIEASDGTKIEGRSSMLRETPKRGDRRVEFRAVEPQR"
     gene            1193..1954
                     /gene="cobM"
                     /locus_tag="VNY06_04735"
     CDS             1193..1954
                     /gene="cobM"
                     /locus_tag="VNY06_04735"
                     /EC_number="2.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008960432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0046026 - precorrin-4
                     C11-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-4 C(11)-methyltransferase"
                     /protein_id="HWX14154.1"
                     /translation="MTVHFIGAGPGAPDLITLRGRDLIARSPVCLYAGSLVPRALLCY
                     CPEGARIVDTAPMSLDEICDEFMRAHRAGEDVARLHSGDLSIWSALGEQIRALGQLSI
                     PFTITPGVPAFAAAAAAIGVELTLPEVAQSLVLTRTPGRASSMPEHETLAAFAATRAT
                     LAIHLSIHMIAQVVAELTPYYGTDCPVAIVFRASWPDQRIVRGTLATIAARAAETQME
                     RTALILVGQVLDPGEFRASALYDRDYQRRFRAKPQ"
     gene            complement(1948..3063)
                     /locus_tag="VNY06_04740"
     CDS             complement(1948..3063)
                     /locus_tag="VNY06_04740"
                     /EC_number="2.1.1.195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012115252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin-5B (C(1))-methyltransferase"
                     /protein_id="HWX14155.1"
                     /translation="MDGNEQNPSPKANLRRGWTTGACAAAAARAAYEALLSGRFPDPV
                     TMTLPGGREPSFALALTELGEGFARAGILKDAGDDPDVTHGALVIARVLHGPAQSGVT
                     FRAGEGVGTVTRPGLPLTPGEPAINPVPRSMIKAAIIEVAATYGGTSDAIVEISIPGG
                     EEIAKRTLNARLGIVGGLSILGTTGIVVPFSCAAWIDTIHRGIDVARASHIDHIAGST
                     GAMSEAAVKALHALPDIALIEMGDFVGGMLKYLRTHKIPKVTIAGGFAKITKLGQGLL
                     DLHSGRGAVDILWLAARGAEAGASADFHARAKSAQSAGEILQIATEENLNVATPVADA
                     AWHTAARVLRGSEIALEIAVFDRSGHLLARTPFLPVH"
BASE COUNT          669 a         1008 c          955 g          667 t
ORIGIN      
        1 gattgagccg gaggccaggc tgagcgcgcc tgagagaacg aggtgagcat gagcgttcca
       61 gaccgcgaaa ccgaagccgg atgccagcgg cgcggagccg gcagcctgga ggtcggaaag
      121 caatcgcctg gcgcgcagaa atttctatcg ctgcggctca actgcgcgaa attcgacccg
      181 acggtctccg cgcttcggcg tttccctgag catgctgctg cgaccctcga ttttcgtgcc
      241 atcgctggcc tcgatagacg cgctgccata acccataggc cttagcttgg ccgggctaat
      301 tcctgcgtga acgagcgccc gatacaccga attcgcgcgc ttacgggaga gagctgtacc
      361 ttgagcagaa tgccagcttc ccgcagtata gccgataacc tcgaccaccg tttccgaagg
      421 tagagttttg attacttccg caaccttcgt tatgactccg aaattgctgg ctggaatcct
      481 tgcgctattg cccgggaatt cgatggtcaa agagttaagc gcggcctgac ccgccgccgg
      541 cactggcccc gctagtctca taagtaggcc gctgtccgtc atcgcggcgg ctacgaatcg
      601 cggaacgcct ggcggtacga agtcaaacga ccgcctgcta cctgggagca ttgtatacga
      661 gctctcatag ccaaagacgc tcaatcttgc cgtgagattc ttcatccaac ctgcttcggg
      721 ggcaaaactg cttgacttgg cggcggtaga ctcggacaca attggcgtgt catcgccccg
      781 gctgccggcc gcaatggcat agccaaatag agcgcatgta accacgaatg ccgagaaagg
      841 aacggctaag cggagcccac cgatgtcact cccggactcc ggcccgagcg gcgaaaattc
      901 tcggttctca tgaacgacaa catcagcttg agtgtcattt ttcaacatcg acatgtttat
      961 tgctccgtcc agggtagcct aaccgccatt attcgcgaaa tgagtttccc gtcctttcac
     1021 gtaaataacc gcattcttgc caaaatggtt cctggaaaaa tgcgggcatc gcactataag
     1081 cactcgcaac gaaaggtgca tgcaataacc gcgaggcttg cgacttctgg cgcccccgcg
     1141 gtccggccaa tggggatttc caaccgctcc cgattgccgg caacaaagtg ccatgacagt
     1201 ccatttcata ggcgcagggc ctggagcccc cgatcttatc acgttgcgcg gccgtgatct
     1261 catcgcccgc tcgcctgttt gcctttatgc aggctcgctc gtgccgaggg cgctcctatg
     1321 ttattgtccg gagggcgcgc ggatcgtcga taccgcgccg atgagccttg acgaaatctg
     1381 cgatgaattt atgcgcgcgc atcgcgccgg cgaggatgtc gcgcgcctgc attcgggcga
     1441 cctttctata tggagcgcgc tcggcgaaca gatccgcgct cttggccagc tctcaattcc
     1501 ctttacgatc acgcctggtg ttccggcatt tgccgccgct gccgctgcca tcggcgtcga
     1561 gctcaccttg ccagaagtcg cgcaatctct ggtcctgacg cgcacgccgg ggcgcgcctc
     1621 gtcgatgccg gagcatgaaa ccctagcggc tttcgcggcc acaagggcaa cgcttgcgat
     1681 ccacctctcg atccacatga ttgcgcaagt tgtggccgag ttgacaccct attatggaac
     1741 cgattgcccc gttgcgatcg tgtttcgtgc ctcctggcca gatcaaagga ttgttcgcgg
     1801 aacattggca accatcgcgg cgcgagctgc cgaaacccaa atggagcgca ccgcgctgat
     1861 cttggttgga caagtcttgg atcccggcga atttcgggca agcgctcttt atgaccgcga
     1921 ctaccagcgg cggttccgag caaaacctca atgaacgggc aaaaaaggcg tgcgcgcgag
     1981 aagatggccg ctgcggtcaa agaccgcgat ttcaagcgcg atttcgctgc cgcgcagaac
     2041 tcttgccgcg gtatgccatg cggcatcagc gaccggcgtt gcaacattga ggttttcctc
     2101 agtggcgatc tgcaaaatct cgccggcgct ttgtgccgat ttcgcgcgcg cgtggaaatc
     2161 tgcgctggca ccagcttccg cgccgcgcgc cgcaagccac aaaatatcaa ctgccccgcg
     2221 gcccgaatga agatcgagaa ggccttggcc gagctttgtt attttggcaa agcctcccgc
     2281 gatcgtcact tttggaatct tatgcgtgcg aagatatttt agcatcccgc cgacaaaatc
     2341 gcccatttct atgagcgcga tatctggcaa agcgtgcagc gctttgactg ccgcttccga
     2401 cattgctccg gtggaaccgg cgatatgatc aatgtgcgag gcacgggcga catcgatgcc
     2461 gcgatggatc gtatcgatcc atgcggcgca ggaaaatggc acgacgatcc ccgtcgtacc
     2521 caaaatcgag agaccgccga caatgccaag ccttgcattg agcgtcctct tggcaatctc
     2581 ttctccgcct ggaatcgaaa tttcgacgat cgcgtcgctc gtaccgccgt atgtggccgc
     2641 gacctcaatg atcgcggcct tgatcataga gcggggcacg ggattgatcg caggttctcc
     2701 gggagtgagc ggcaggcccg ggcgcgtgac cgttccaacc ccttcgccag cacggaaagt
     2761 aacgcccgac tgggccggac catgcaaaac ccgggcgatc accagcgcgc catgcgtcac
     2821 atcggggtca tcgcccgcat ccttgagaat gcctgcgcgc gcaaagccct cgccaagctc
     2881 cgtcaaggcc aaagcgaagg atggctctct gccgcccggc agcgtcatgg tgacgggatc
     2941 gggaaagcgg ccggaaagga gcgcctcata ggccgcgcgc gcggcggcag cggcgcaagc
     3001 gccagttgtc cagcccctgc gcaggttcgc cttcggtgag ggattttgct cgtttccgtc
     3061 cacgggatag agagcgcctc cgagagggtg tccggaggca aacccggttc cctcttttgc
     3121 cggatacgct cgcataaatt tcgcggcgaa ggcaacgcag ctcagtctgc atggacggct
     3181 ttgccggagc gcttttcagg agcgctcgag aagcggcttg ttttcgactt ttggatagtg
     3241 aggcaaatta ggcggaatgc cgagatcttt catgcgcaga gagcgctcat aggctcgat
//
LOCUS       DATMPU010000205         2947 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_3259060,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000205 DATMPU010000000
VERSION     DATMPU010000205.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 2947)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2947)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2947
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3259060"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..363)
                     /locus_tag="VNY06_04745"
     CDS             complement(<1..363)
                     /locus_tag="VNY06_04745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX14156.1"
                     /translation="MAWAGRKRKNGHRWANGNLKQPNAAERHRSQRQIEDAEKAVVLA
                     QPHRRGDKSQNCESPLGRFAIKWKLRHEVFDAGLQYGRMVRLWQVNHGVQIEVHEPSA
                     CHSGAEIDAATMRRWNAEI"
     gene            637..801
                     /locus_tag="VNY06_04750"
     CDS             637..801
                     /locus_tag="VNY06_04750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX14157.1"
                     /translation="MVRKLARLYPQATPRPYPARRQWVIDDEDTDEMVHSICGWLRPI
                     EQKQERSGHG"
     gene            complement(868..2085)
                     /gene="cbiE"
                     /locus_tag="VNY06_04755"
     CDS             complement(868..2085)
                     /gene="cbiE"
                     /locus_tag="VNY06_04755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014892594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046025 - precorrin-6Y
                     C5,15-methyltransferase (decarboxylating) activity
                     [Evidence IEA];
                     GO_process: GO:0046140 - corrin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-6y C5,15-methyltransferase
                     (decarboxylating) subunit CbiE"
                     /protein_id="HWX14158.1"
                     /translation="MTAPFCEKWLALIGIGEDGIEALSPAARKLLAQALLIVGGARHL
                     ALAGPLACETMIWPTPLTDAIPAILARRGSPVCILCSGDPFHYGAGTLLSAHFGRDEM
                     QCFPAPSAFSLAAARLNWSLQDCSLISLHGRSFERIIPVLQPGARILCLSYETTPPRL
                     AKLLCARGLGETGMIVLEAMGGPRERMRETLASNFDLGEIAPLNVVALEIPLKNAVQF
                     LPRANGLADSWFTTDGQFTKREVRAITLSSLAPMRGELLWDVGAGSGSIAIEWLLSDP
                     ANRAIAIEAREDRTSLIKRNAENLGVPHIETVTGKAPEAFANLPQPQAIFVGGGASNK
                     ALLDKAYAALPEGGRLVVNAVTLEAEAELIRRFKMQGGDLTRIEISRAKSLGSFEAWR
                     PALPVTQWSVTKR"
     gene            2118..2873
                     /locus_tag="VNY06_04760"
     CDS             2118..2873
                     /locus_tag="VNY06_04760"
                     /EC_number="1.3.1.106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012592239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016994 - precorrin-6A reductase activity
                     [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin-6A reductase"
                     /protein_id="HWX14159.1"
                     /translation="MRVLVLGGTSEANKLAHLLAEASGISATVSFAGRTKAILAPPIS
                     CRIGGFGGVEGLIDYLKAENIDVLIDATHPFAEQISRHAEIAAKRAKIPLAVLSRPAW
                     VRTVQDLWIDAADKTDAALALGHEPRRVFLTIGRLQIEAFAKAPQHFYLVRTIEPLAL
                     PPRLPNYRVIHGRGPFVVEAEEKLLREESIDVVVTKNSGGEAAFAKILAARNLRIPVI
                     MVARPGEAMQAAMHDPGEVMDCILRHQLARRGV"
     gene            complement(2849..>2947)
                     /locus_tag="VNY06_04765"
     CDS             complement(2849..>2947)
                     /locus_tag="VNY06_04765"
                     /EC_number="2.1.1.131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008967816.1"
                     /note="catalyzes the formation of precorrin-4 from
                     precorrin-3B and S-adenosyl-L-methionine; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-3B C(17)-methyltransferase"
                     /protein_id="HWX14160.1"
                     /translation="DMSTLIIIGSSATRIIPRNNAAPFVYTPRRAS"
BASE COUNT          618 a          862 c          892 g          575 t
ORIGIN      
        1 gatctcggca ttccagcggc gcatcgtggc ggcatctatc tcggcgcctg agtgacatgc
       61 ggatggctca tgcacctcga tctgcacacc gtggttgacc tgccatagcc tcaccatgcg
      121 gccgtactgg agcccggcgt cgaagacctc gtggcgcagt ttccacttga tcgcgaagcg
      181 ccccagtgga ctttcgcagt tttgcgactt gtcgcctctg cggtgcggct gcgccagaac
      241 cacagctttt tcggcatcct cgatctgtcg ctggctgcga tggcgctcgg cggcattcgg
      301 ctgcttgagg ttgccgttgg cccagcgatg gccgttctta cgtttccgtc cggcccatgc
      361 catgcgtcct cgcgaaccaa ggcgtgcgcg ctaaatgcgt gttcatgtcg cgtgtaacga
      421 aatgcaccaa aatgaaacca aagcgaaagc aacggtatac agatgtaaca aaatgcaaca
      481 aaaagccgcc cagaaggggc ggctcagttt caaaaacttc aatgaaatca ctatgatagt
      541 ggaggcatta cttgagcttt acgcgggcgc agcgaacgga tacgctgtcc ggtctatatc
      601 gtcagcggcg gcaaggactg gctgacaccg gcctcggtgg tgcgcaagct ggcgcgcctc
      661 tacccacagg ccacgccgcg cccctacccg gcacgccgac aatgggtgat cgacgacgag
      721 gacaccgacg aaatggtgca cagcatctgc ggctggctgc ggccgatcga acagaagcag
      781 gagcgcagcg gacatggctg agcgctcgtc gatgcacaga aggcgtcgaa ggaaccattc
      841 accatgatta tggcatgaaa tgcaatgtca ccgcttcgtc acagaccatt gcgtgacggg
      901 gagggccggg cgccaggctt caaaggagcc gagagatttt gcgcgcgaga tttcgatgcg
      961 cgtaagatcg ccgccttgca tcttgaaacg gcggatgagc tccgcctcgg cctcaagggt
     1021 caccgcattg acgacgagcc tgccgccttc cggcaaggcg gcataggctt tgtcgaggag
     1081 ggctttattg cttgctccac cgccgacgaa gatcgcctgc ggctgcggca gattggcaaa
     1141 agcttccggc gctttcccgg ttacagtttc aatatgcggc acgccgaggt tttcggcgtt
     1201 gcgcttgatg agacttgtac gatcttcccg cgcctcaatc gctatggcgc gattggcggg
     1261 gtcgctcaga agccattcga tcgcgattga tccggatccc gcgccaacgt cccacaaaag
     1321 ctcgccgcgc attggcgcga gcgacgataa ggtgatcgcg cgcacctcgc gtttcgtaaa
     1381 ctggccatca gttgtaaacc aggaatcggc aagaccgttc gcccggggaa gaaattgcac
     1441 ggcgttttta agaggaattt cgagcgccac gacgtttagg ggggcgattt ctcctaggtc
     1501 aaagttcgag gccaaagtct cgcgcatgcg ctcccttggg cctcccatcg cctccagcac
     1561 aatcatccct gtttcaccaa gtccgcgcgc gcaaagaagt tttgcaagcc ggggcggagt
     1621 tgtctcgtag ctgaggcaaa ggattcttgc acctggctgc agaacgggaa taatccgctc
     1681 aaagctgcgc ccatgcaaag agatgaggct gcaatcttgc aggctccaat tgagccttgc
     1741 cgctgcgagg ctaaaagccg aaggcgctgg aaaacactgc atctcatcgc gcccgaaatg
     1801 ggcactcaac agcgtcccgg cgccgtaatg gaaaggatcg cccgagcaaa ggatacagac
     1861 gggagagccg cgccttgcga gaattgccgg aatggcgtca gtgagcggtg tcggccaaat
     1921 catggtttcg caggcaagcg ggcccgcaag cgcaagatgg cgagcgcctc ccacgatcaa
     1981 gagcgcttgc gcgagcaatt ttcgtgcggc aggagagaga gcctcgatac catcctcgcc
     2041 gatgccaatc agcgccagcc atttttcgca aaaaggcgcg gtcaccgcgg aatgagccct
     2101 aatgggagag ccgaaggatg cgcgttttgg tgctcggcgg gacatcggag gcgaataagc
     2161 tcgcgcatct cctcgccgaa gcgagcggca tctccgccac ggtgtctttt gccggccgca
     2221 caaaggcaat ccttgcgccg cccatttcct gtcggatcgg cggctttggc ggcgtcgagg
     2281 ggcttataga ctatcttaaa gccgaaaaca tcgatgtgct catcgatgcc acgcatcctt
     2341 tcgccgaaca aatttcgcgc cacgcggaga ttgcggcgaa gcgggcaaaa attccgcttg
     2401 ccgttctttc gcgcccggcc tgggttcgaa cggtgcaaga tctttggatc gatgccgccg
     2461 acaagacgga tgccgctttg gcccttgggc acgaaccgag gcgggttttt ttgacgatcg
     2521 ggcggctgca aatcgaggcc ttcgcgaaag ccccccagca tttctatctt gtccgcacca
     2581 tcgagcctct cgcgcttccc ccgcgcttgc caaactaccg ggtcattcac gggcgtggcc
     2641 ctttcgtggt cgaagcggag gaaaagcttt tgcgcgaaga atcgatcgat gtggttgtga
     2701 caaagaacag cggcggcgag gcggctttcg cgaaaattct cgccgcgcga aaccttagga
     2761 ttccggtgat catggtcgcg cggcccggcg aggctatgca ggcggcaatg cacgacccag
     2821 gggaagtcat ggactgcatc cttcgtcatc agcttgcccg acgcggggtg taaacgaagg
     2881 gcgcggcatt gttacgcgga ataatgcgcg ttgcgctcga accaatgatg atgagggtgc
     2941 tcatatc
//
LOCUS       DATMPU010000206         2941 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_3575474,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000206 DATMPU010000000
VERSION     DATMPU010000206.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 2941)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2941)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2941
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3575474"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..464)
                     /locus_tag="VNY06_04770"
     CDS             complement(<1..464)
                     /locus_tag="VNY06_04770"
                     /inference="COORDINATES: protein motif:HMM:NF018745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1345 domain-containing protein"
                     /protein_id="HWX14161.1"
                     /translation="MPESLRLSTRLLIAWNAGTWFYFIATGVMIARASPQSIRHRAKR
                     TDEGKSFILVSTTLAAIAAIAAIMVELAAVAATEDLPKTLKGLHIGLAAATIISAWFF
                     IHLTYALHYAHEYFDENVAESGRPATERGGLIFPGNEEPDYYDFLYFSYVIGV"
     gene            complement(593..1525)
                     /locus_tag="VNY06_04775"
     CDS             complement(593..1525)
                     /locus_tag="VNY06_04775"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012383434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004337 - geranyltranstransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="farnesyl diphosphate synthase"
                     /protein_id="HWX14162.1"
                     /translation="MTTVTLASEFEARLAEAAETTELMLDSLLGPAILRGETLRPPRF
                     LEAMRYASLDGGKRFRPFLTIETARLLGAAGKGVQRAAAAIEMVHCYSLVHDDLPALD
                     NDDLRRGRPTTHKAFGEANAILVGDALLTYAFDVLADEATHPEPQTRIDLVLGLARAA
                     GVGGMIGGQVLDLEAEEARESHTAETVTRLQSMKTGTLLHFAVDAGAILGGAKAPERA
                     ALSRFGRALGAAFQISDDILDVEANERELGKRAGKDAGRNKATLVAALGIAAAREQRD
                     KLVTEAIAALDSFSLKTKTTVLKEAARFVAARTH"
     gene            1601..2296
                     /gene="mtgA"
                     /locus_tag="VNY06_04780"
     CDS             1601..2296
                     /gene="mtgA"
                     /locus_tag="VNY06_04780"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006936670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HWX14163.1"
                     /translation="MASKSRRKGLAGMILRTVLGVGLGLVLGFAVLIFVYRFLPPVST
                     LMLVRWIERETAERHYVPLERISENLRKAVITSEDARFCRHHGVDWSALRLVIKHPGA
                     AGPSRGASTIPMQTAKNLFLWPSRSYLRKAIEIPLALLIDFEWPKKRTLEVYLNLAQW
                     GQGIFGAEAAARHYFHKSAEKLDEKEAVLLATALPSPQHRNPAKPSPRQAGLAARLMG
                     RVRTAGPVADCIL"
     gene            2466..2654
                     /gene="rpmF"
                     /locus_tag="VNY06_04785"
     CDS             2466..2654
                     /gene="rpmF"
                     /locus_tag="VNY06_04785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003601373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HWX14164.1"
                     /translation="MAVPKRKTSPMKRGFRRSADALKAPTYVEDKDSGELRRPHHVDV
                     KTGMYRGRQIFTQKSNEA"
BASE COUNT          644 a          825 c          860 g          612 t
ORIGIN      
        1 acaccaatta cataggaaaa atacagaaag tcataataat cgggttcctc gtttccagga
       61 aaaatcaggc cgccccgttc ggttgccggc ctgcctgatt cggcgacatt ttcgtcgaaa
      121 tattcatgcg cgtaatgcag tgcgtaggtg agatgtatga agaaccaggc gctgatgatc
      181 gttgccgccg caagtccgat atgcaggccc ttaagcgtct ttggtaaatc ctcggtggct
      241 gccacggcgg cgagttcaac catgattgcc gcgatcgcgg caatcgctgc taaagtagtg
      301 gagaccagaa tgaaggattt cccctcatca gttcttttag cccgatgccg gatcgattgc
      361 ggagacgccc gcgcgatcat aacgcccgtg gcgatgaaat aaaaccaggt tcccgcattc
      421 caggcgatca agaggcgtgt cgaaagccgc aaactttccg gcaaaaagaa ctccacggcg
      481 aagcccgcag cgacgcccgc gaagagacgc ggatggctgc gcacaacttg aaggggaagc
      541 cgaattcggt taaggcgccg ccgctgggtg ggtatcggcc tgcggcgagg cattaatgcg
      601 ttcgcgcagc gacgaatcgg gccgcctctt tcaggaccgt tgtcttggtc ttcagggaga
      661 agctatcgag cgctgctatt gcttccgtca ccaatttgtc gcgctgttcc cgcgcggccg
      721 caattccaag tgccgcgaca agagttgcct tgttgcggcc cgcgtctttg cctgcgcgct
      781 tgccaagctc gcgttcgttt gcctcgacat caagaatatc gtcggaaatc tgaaaagcgg
      841 caccaagcgc gcggccgaaa cgcgagagcg ccgcccgttc gggagcttta gcgcctccaa
      901 gtatagcccc ggcatccact gcaaaatgca agagcgtacc ggtcttcatc gactgaagtc
      961 ttgttaccgt ttcggcggta tgcgattcac gcgcctcctc ggcttcaagg tcgagaactt
     1021 gcccgccaat catgccgccc acaccagcgg cgcgcgcaag accaagtacg agatcgatgc
     1081 gcgtttgcgg ctcagggtgg gttgcctcat ctgccaaaac atcgaaggcg taagtgagga
     1141 gcgcatcgcc gaccagtatc gcattggcct cgccgaacgc cttgtgggtg gtgggtcgtc
     1201 cccggcgcaa atcgtcattg tcaagggccg ggagatcgtc atggacaagc gagtagcaat
     1261 gcaccatttc aatcgccgct gcggcgcgct gcacgccttt gcctgcagcg ccaagtaatc
     1321 ttgcggtctc gatcgtgaga aaaggccgga acctcttgcc gccgtcgaga ctggcgtaac
     1381 gcatggcttc gagaaatcgt gggggacgga gggtttcgcc ccgcaagatg gcaggtccga
     1441 gcagagagtc gagcataagc tctgtcgtct ctgctgcttc cgccagccga gcctcaaatt
     1501 cgcttgcgag cgtgaccgtt gtcattgtct tgctgtccct gtccccaata tagtctagtc
     1561 catgcttgcc cgattgagct cagctggtca aggcttggtc atggcatcga agtccaggcg
     1621 gaagggtttg gccggaatga tcctgcgcac ggtgctcggc gtcggcttgg ggctggtgct
     1681 cggttttgcg gttttgatct tcgtttaccg ttttctcccg ccggtctcga cgcttatgct
     1741 tgtgcgctgg attgaaagag aaacggctga acgtcactac gtgccgcttg agcgcatctc
     1801 ggaaaatctg cgcaaagccg ttattacatc ggaggatgcg cgcttttgcc gccatcacgg
     1861 cgttgactgg agcgcgcttc gactggttat caaacatccg ggcgcggccg gaccgtcgcg
     1921 tggcgcctcg accattccca tgcagaccgc aaaaaatttg tttctttggc catcgcgatc
     1981 ctatctgcgt aaggcaatcg aaattccgtt ggccctgtta attgactttg agtggccgaa
     2041 aaagcgcacc ctggaggtct acctcaacct tgctcaatgg ggacaaggaa tatttggcgc
     2101 tgaggcggcg gcgcggcact acttccataa gagcgcggaa aagctcgacg aaaaagaggc
     2161 ggtgctattg gcgacagcct tgcccagccc ccagcaccgc aatcctgcta agccgtcccc
     2221 gcgccaggcc gggctcgcag cgcgtctcat ggggcgggtg cgaacggccg gtcccgtcgc
     2281 agattgcatt ttatgaggct agcatctcac tgagtcgacg ggcgagccca acatttgcgc
     2341 gtagttgtag tttgggatgg ctaggcgttg gaatttattg taagaggcaa cctcaattcg
     2401 ctaatgcaca acgcagtttc gacgcgaact tcatcgaaga taatgacacg acagagagaa
     2461 aaaggatggc agttccgaaa cgcaaaacct cccccatgaa acgcggattc cgtcgctccg
     2521 ccgatgcgct caaggcgcct acctatgtgg aggacaagga ttccggtgaa ttgcggcgtc
     2581 cgcatcacgt cgatgtcaag acgggcatgt atcgcggccg gcagattttc acccaaaagt
     2641 ccaatgaagc gtgaagccga attcgctgac tgctgagagt aggggcgctt gaccgatctt
     2701 tggcagccaa gcgcgtgtta agccaaaagt ggaaaccggt tttgcgtccg gaaacgcaac
     2761 aaaacaacta agacacatgc gccaccagtc ttctacgaaa acgtcggcga aacaacacaa
     2821 ggcgtaacgc gcaacttgtg caccggtgtc atcggaaccg aggacaccgg gctcttgcgg
     2881 agcatttatt tggacgttta gacgttggaa ggcgaaccgg gcagggagga ctcgaagaag
     2941 c
//
LOCUS       DATMPU010000207         4106 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_2033425,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000207 DATMPU010000000
VERSION     DATMPU010000207.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 4106)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4106)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4106
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2033425"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..676
                     /locus_tag="VNY06_04790"
     CDS             <1..676
                     /locus_tag="VNY06_04790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX14165.1"
                     /translation="VRLRSALLIFGICLGLCGTWILVPALLAPKPNGLPLDRVLADAA
                     STRRPRAVLAAGIGAIRGDLWAEAAFTGAHFMWQDPATILDETASRQLNRVRANAEMT
                     LVWAPVNSAVWLLLAMLPQNPSDRDSPVSTLLELSYFTAPNDLSLGPLRVMRAATSSA
                     LANKDIQLFVKSDIRGILDREPDHQQDIIAAYRKAWPQNQPIFENLVADIDPAVATLL
                     SSPQSK"
     gene            836..2053
                     /locus_tag="VNY06_04795"
     CDS             836..2053
                     /locus_tag="VNY06_04795"
                     /EC_number="1.1.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013947041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006102 - isocitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HWX14166.1"
                     /translation="MNKIKVENPVVELDGDEMTRILWHYIRDKFIHPYLDVKLEYYDL
                     SIENRDATNDRVTAEAANAIKKHGVGVKCATITPDEARVEEFKLKQMWKSPNGTIRNI
                     LGGVIFREPIICQNVPRLVPGWTRPIVIGRHAFGDQYSATDFKIPGRGRTVIRFEGED
                     GTIIERKIYDYPGPGVSLSMYNLDDSIRDFARASMNYGLMRKYPVYLSTKNTILKAYD
                     GRFKDLFQEVYETEFKAAFEKAGLTYEHRLIDDMVASSLKWSGGYVWACKNYDGDVQS
                     DTVAQGFGSLGLMTSVLMTPDGKTVEAEAAHGTVTRHFREHQKGKETSTNSIASIFAW
                     TRGLAHRAQLDGNNELTIFAETLEATCVATVEAGYMTKDLALLVGADQRWLSTTGFLD
                     KISENLHAKLSQG"
     gene            complement(2075..3367)
                     /locus_tag="VNY06_04800"
     CDS             complement(2075..3367)
                     /locus_tag="VNY06_04800"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018183004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HWX14167.1"
                     /translation="MYRGCNHLLPSKRIVRSLLDTDFYKFLMLQMIWRFKAETHVTFA
                     VLNRSPTIRLAETIADSDLAAELDHARGLRFQKDELIWLATTSFYGKAGLFDPAFLDY
                     LASFRLPPYELSRRNGEFELRFPGLWRETTLWEIPALAILSELFARTAVKGLSHLQVD
                     TLYNCANGKLWDKVKRLQALAREGPLAITDFGTRRRHGFLWQRWCIEALREGLQQSFI
                     GSSNVKFAMDLGVEAIGTNAHELPMVYAALSESDEGLLQAPFKVLVDWAKLYDGDLRV
                     LLPDTFGSAHFLAAAPDWAVDWAGARPDSKPPIEAGEELIEWWARRGRDPAAKRIILS
                     DGMTVESIESAVRQLRGRAKLSIGWGTNLTNDFSDCWPSGSGAKSMPASIVCKVVEAE
                     GRPAVKLSDNPGKVLGPPEEVARYERVFNYCHEKSRPA"
     gene            complement(3471..>4106)
                     /locus_tag="VNY06_04805"
     CDS             complement(3471..>4106)
                     /locus_tag="VNY06_04805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012336321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HWX14168.1"
                     /translation="QLTCEVDVAESAHGSAARALSEARKNAAARLDEAVGAELAPLKL
                     EGAIFKTEFGTAPEGPDGYDQVEFWVRTNPGTNPGPLVKIASGGELARFMLALKVVLA
                     DRGSAPTLVFDEIDTGAGGAVADAIGQRLARLASGVQVLAVTHAPQVAARAGEHFRIV
                     KDSPGRGDRVATRVSVLGKEARREEIARMLAGATITDEARAAAARLLASAQ"
BASE COUNT          906 a         1189 c         1139 g          872 t
ORIGIN      
        1 cgtgaggctg cggagcgcgc tcctgatttt tggcatttgc ctcgggctat gcggaacatg
       61 gatattggtt cccgcattgc tggcgccgaa gccgaacggt ctaccgcttg atcgcgttct
      121 ggccgacgcc gcctcgacac ggcggccgcg tgcggtcctc gccgcgggaa tcggcgccat
      181 cagaggagac ctgtgggccg aagctgcctt cacaggcgcc cacttcatgt ggcaggatcc
      241 tgcgactatt cttgacgaga ccgcctcaag gcagcttaac agggtcagag ccaacgcgga
      301 aatgacgcta gtttgggcgc cagtcaatag cgcggtatgg cttcttttgg ctatgctgcc
      361 gcaaaaccct tcggatcgcg acagcccagt ttcaactttg ctcgaattgt cctatttcac
      421 cgcacctaat gatctaagcc ttggcccact gcgggtcatg cgggcggcaa catctagtgc
      481 gctggccaat aaggacatcc agttattcgt aaagagcgac atcaggggta ttctcgaccg
      541 tgagccggat catcaacaag acatcattgc cgcctatcgc aaagcttggc cgcaaaacca
      601 gcctattttc gagaacctcg tggccgacat agaccccgcc gtggccacgc tgctgagctc
      661 accgcaatca aaatgaggac aaagttcggc tgcggcctcc gggaaaaacc aatatcaaat
      721 acatttgtcc gctctggccc gtgcggactt ggactttagg cacgtgcggt ttgactgatg
      781 ccatgccgag ccggtccgga ctggcaattc cgcaaaatag cggagggcac tactgatgaa
      841 caagattaag gtcgaaaatc ccgtcgtcga acttgacggc gacgaaatga cgcggattct
      901 ttggcattac atacgcgaca agttcatcca tccctatctc gatgtaaaac tcgaatatta
      961 cgacctctcg attgaaaacc gagacgcgac gaatgatcgc gtcacagccg aggcggccaa
     1021 tgccatcaaa aagcacggcg ttggcgttaa atgcgcgacc attacgcccg acgaagcgcg
     1081 agtggaagag tttaagctca agcaaatgtg gaaatcgccg aatggcacga tacgcaatat
     1141 tctgggaggg gttatctttc gtgagccgat catttgccag aatgtccccc gtcttgttcc
     1201 cggttggaca cgcccgattg tcataggccg ccatgcgttt ggcgatcaat acagcgcaac
     1261 ggatttcaag atcccgggca gaggccgtac cgtcatcagg ttcgaggggg aagacggcac
     1321 gattattgag cggaagattt acgactaccc tggcccgggc gtttccctgt cgatgtataa
     1381 tctcgatgat tcgatacgtg actttgcccg tgcgtcaatg aattacgggc tgatgcgcaa
     1441 ataccccgtc tatctgtcga caaagaacac aattttgaaa gcctatgatg ggcgattcaa
     1501 ggatctgttt caggaggttt acgagaccga attcaaagcc gcgtttgaga aggctggact
     1561 gacttatgag caccgcctga tcgacgacat ggtggcctct tcgctcaaat ggtcgggcgg
     1621 ttatgtgtgg gcgtgcaaga actatgatgg tgatgtccaa tccgatacgg tcgcgcaggg
     1681 atttggatcg ctcggcctca tgacctcggt gctcatgacg cctgacggca agacggtcga
     1741 agccgaggcc gcgcatggca cggtcacacg tcattttcgc gaacatcaaa agggcaaaga
     1801 aacgtcgacc aattcaattg cgtctatttt cgcctggaca cgcggtttgg cgcaccgtgc
     1861 ccagctcgac ggcaataacg aacttacgat attcgcggag acgctggagg cgacatgcgt
     1921 cgccacggtc gaagcaggct acatgacaaa agacctagcc ctgcttgtag gagccgacca
     1981 gcgctggctt tcgacaacgg gtttccttga taagatcagc gaaaatttgc acgcaaagct
     2041 aagccaaggc taggtcattc tggctttctg gacttcaggc cggccgagat ttctcgtggc
     2101 aatagttaaa aacccgttcg tagcgggcaa cctcttccgg aggtcctagg accttacccg
     2161 gattgtcgga aagcttcacg gctggacgcc cctcggcttc gacaaccttg caaacaatcg
     2221 acgccggcat ggatttcgcc cccgacccgc tcggccagca atcagagaaa tcattggtga
     2281 ggttggttcc ccatccaata gaaagctttg cccgtccgcg caattggcgc actgcggatt
     2341 cgatggattc gacagtcatg ccgtcggaaa gaatgatgcg cttcgccgct ggatcccgtc
     2401 cccttcgtgc ccaccattcg atcagttctt ctccggcttc aatgggtggt ttggaatccg
     2461 gccgcgctcc cgcccaatcg acggcccagt ccggagctgc ggcgagaaag tgcgcgcttc
     2521 cgaatgtgtc gggcaatagg acccgaagat cgccgtcata gagcttagcc caatctacta
     2581 ggaccttaaa gggcgcttgg agcaagcctt catcggattc cgaaagcgcc gcatagacca
     2641 tgggcagttc atgggcattg gttccgattg cctcaactcc gaggtccatc gcgaatttaa
     2701 cattcgatga gccaatgaag ctttgttgaa gaccttcccg caaggcctcg atgcaccatc
     2761 gctgccacaa gaagccatgc cgccggcgtg ttccaaagtc ggttatggct aacggccctt
     2821 cccgtgcgag cgcctgcagc cgtttgacct tgtcccagag cttcccgttc gcacagttgt
     2881 acagcgtatc aacttgcaaa tggctcaagc ccttcactgc cgtgcgggca aaaagttcgc
     2941 tcaagattgc aagtgcggga atttcccaca gagtcgtttc gcgccaaagt cccggaaagc
     3001 gaagttcgaa ctcgccattg cgcctggaaa gctcatacgg cggcagccga aatgacgcga
     3061 gatagtccag gaaagcaggg tcaaacaatc cggctttgcc gtagaagctg gtggttgcaa
     3121 gccagatcag ctcatccttc tgaaatcgaa gaccacgcgc atgatcgagc tccgcggcga
     3181 gatcgctgtc ggcgatggtt tccgcaagcc ttatcgtcgg ggaccggttc aatactgcaa
     3241 aagtaacatg cgtctcagcc ttgaaccgcc aaatcatttg cagcatgagg aatttgtaga
     3301 aatccgtatc gagcaggctc cgtacgatcc gcttgctggg gagcaaatga ttgcagcccc
     3361 tatacatgtt gcctaatatc atacgaaacg cgcgctccgc ttcgagttgc gcggggtgcc
     3421 ttctttcgcg cagtgcgcga ccctgagttg gcgatacgtc tcatttgttt tcattgtgcg
     3481 ctagccaata gccgcgccgc ggcagcccgt gcttcgtcgg tgatggtcgc gccggccagc
     3541 atgcgcgcga tctcctcgcg ccgcgcttcc tttccgagaa ccgagacgcg cgttgcaact
     3601 ctatcacctc gcccggggga atcttttaca atgcggaaat gttcgccggc ccgcgccgcg
     3661 acttgggggg catgcgtcac ggcgagcact tgcacgcccg acgcaaggcg cgcaagccgc
     3721 tggcctatgg catcggcaac cgcgccgccc gcgccagtgt cgatttcgtc aaagacgagc
     3781 gttggcgccg agccgcgatc ggcaagaacg actttaaggg ctagcatgaa acgggcaagt
     3841 tcgccgcccg atgcgatttt caccaaaggg ccgggatttg ttcctgggtt ggtccgcacc
     3901 caaaattcca cttggtcata tccatccgga ccttccggcg cggtgccaaa ctccgtcttg
     3961 aaaatagcgc cttcgagttt gagcggggcg agttccgcac caaccgcttc gtcgagccgg
     4021 gccgcggcat ttttccgagc ctcggacaat gcccgcgccg ctgatccatg cgcagattcc
     4081 gcaacgtcga cctcgcacgt caattg
//
LOCUS       DATMPU010000208         3370 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_1098895,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000208 DATMPU010000000
VERSION     DATMPU010000208.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 3370)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3370)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3370
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1098895"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            296..1732
                     /locus_tag="VNY06_04810"
     CDS             296..1732
                     /locus_tag="VNY06_04810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX14169.1"
                     /translation="MTWPAIVALVLIAICLIPRSPLPLLYLSFILSTITTLSLNPIEG
                     GTNILPGAVCAAFFVCKIVLAGDRLPKAVDAAIEPAKLNILFVFLAYALFTAYVLPRL
                     FARMVEVVPINPDSSWPVALTPTSANYSQSAYLALSVGVTLAFFLQGENSSFRRHFIQ
                     AVLVGGLSLIVTGIADFTLTALGDTDLLEPFRNAKYALLTNAEVEGDKRIVGLMPEAS
                     AFGSSCVASVATLAFLRPCLENRWLRNLLAPLTIFGLVAMAVGSLSSTAYGGLGVFGA
                     AYAANWLRRALSPDAPAREALKWEAIAVVAAVVILLAIVALAPDLMKPIYERLDALIF
                     KKSESTSFDDRTRFTKTALNAFFATDGLGVGFGSVMTSNWFVAILSSTGIFGAVLLFI
                     FILRLYILRCRSADPRAREFAMGLKFALVPYFAMQAAVGTTPDIGVPAASAMGLLASL
                     TSTDEKSSLGRVAAGGWPIRARNRPSSS"
     gene            1792..2682
                     /locus_tag="VNY06_04815"
     CDS             1792..2682
                     /locus_tag="VNY06_04815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009493284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HWX14170.1"
                     /translation="MTSPLLIFDRSLARRRLARAAASGKGDDFLLARAIEEFRDRLST
                     IKRAFSEVLDAGTPAPRLAAWLVDRFQPRLLVRMAPLPETAGKIPLVCLIGDEENLPL
                     AQESFELVVTALSLQNLNDLPGALIQLRRVLRPDGLFLGCLLGGTSLHELRTVLAVAE
                     TELCGGISPRVAPFADVRELGGLLQRAGFALPVADSETVTVRYASLFDLMADLRAMGA
                     TNALVARLRKPSAKGFFMRAAEVYAQRFADPDGRLRATFEMIFLSGWAPHESQQKPLP
                     PGSAKMRLSDALAIQKANRK"
     gene            complement(2687..3229)
                     /locus_tag="VNY06_04820"
     CDS             complement(2687..3229)
                     /locus_tag="VNY06_04820"
                     /inference="COORDINATES: protein motif:HMM:NF016300.4"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HWX14171.1"
                     /translation="MRGLDKVRAEFSLTALVYNLRRALNILGVARLIDEVRDATGYIT
                     KRTLRGYIANNSLTVRIKDIGKAGTLAQQMLDKGANRFDGIAYQYSQKEEKIDSLRGD
                     AVRDAMRKANSYVKAIGIRLGRVLEIASQPHAPVPVGMAPRMRAAAAPAQEATLVIPV
                     EPGIETLRTEVQVTRELAED"
BASE COUNT          634 a          973 c          952 g          811 t
ORIGIN      
        1 cgggaaaaat gccgttgcaa ataaatcgga aattatggaa tatcttagaa tcggtggatg
       61 gatcctacaa aggccgcatg gtgtgcggca tttgcagggg cgactctaga acaggccgga
      121 tatccggatt tagcatcaaa cgtcgcaacg tcttacgcaa cgtacgggca ggtgtaccgc
      181 ttcttctgcc aataagagcc ccgggctatg gtgacagcaa aatggccttt aaaatcagct
      241 tacgagattt gatctcgccg gcccttcgag ggcccagcct caccgaagct tgccgatgac
      301 ttggcccgcc attgtggcac tggtgttgat tgccatctgc ctgattccgc gcagcccttt
      361 gcctttgctc tacctctctt tcatcctaag cacaatcacg accctttcgt taaatccaat
      421 cgaaggcggc acaaatatcc tccccggggc cgtctgtgcg gcattctttg tttgcaagat
      481 tgtacttgcc ggggaccgtt tgccgaaggc cgtggacgcc gccatcgaac ctgccaagct
      541 caacatcttg tttgtttttc tcgcctacgc cctgtttacc gcctatgtgc tgccccggtt
      601 gttcgcccgg atggttgagg tcgtcccaat caacccggac tcgagttggc ccgttgcgct
      661 tacgccgaca tccgccaatt acagtcagtc tgcctatctg gcattgtcgg tcggcgtaac
      721 attggccttt tttctccaag gcgagaacag ttccttccgg cgccacttca ttcaggcggt
      781 cctcgttgga gggctgagct tgattgtcac gggtatcgcc gatttcaccc tgactgctct
      841 cggcgatacc gaccttttgg aaccttttcg caatgccaaa tatgcgcttc tgacgaatgc
      901 cgaggtggaa ggcgataagc gcatcgtggg cctaatgcca gaagcgtcgg catttggcag
      961 ctcttgcgtt gcgtcggttg ccactctggc ctttctgcgg ccctgcttgg aaaatcggtg
     1021 gttgcgcaat ctgctggccc cgctcaccat cttcgggctg gtggcgatgg cagtgggttc
     1081 tctgtcgtcc accgcttacg gaggtcttgg ggtattcggg gcggcttacg cggcaaactg
     1141 gttgcgccga gcgctgagcc cggacgcgcc cgcgcgggaa gcgctgaaat gggaggcaat
     1201 tgccgtggtg gcggcggtgg tgatccttct tgcaattgtt gcgctcgctc ccgatctgat
     1261 gaaaccgatt tatgagaggc tcgacgctct cattttcaag aaatccgaat cgacttcctt
     1321 tgacgaccgc accagattca cgaagacagc tctaaacgcc tttttcgcca ccgatggatt
     1381 gggcgttggc tttggctcgg tgatgacatc gaactggttc gtcgcgattt tgagcagtac
     1441 cggcattttt ggcgcggtgc tgctttttat ctttatcctc cgtctttaca tcctgcgctg
     1501 ccgcagcgct gatccgaggg caagagaatt tgccatgggg ttgaaattcg ccttggttcc
     1561 atattttgcg atgcaggcag cggtggggac gacgccagat ataggagtcc cggcagcgtc
     1621 agcaatgggg ctactcgcaa gcctaacctc aacagatgag aagtcatctc ttggcagggt
     1681 ggccgcaggc ggctggccta tacgagcaag gaaccgtcct tccagctctt aacttgatcg
     1741 ttaagttccg atttcacggt ctttaatgtt taagcctcag tgcagcgcag aatgacatca
     1801 cctttgctga tttttgaccg cagcctcgca aggcgcaggc ttgcccgcgc cgcggcttcc
     1861 ggaaagggtg acgactttct tcttgcccgg gccatcgaag aatttcgcga ccggctttcg
     1921 acaatcaagc gggcattttc tgaagttctc gacgctggaa cgcccgcgcc gcggcttgcc
     1981 gcttggctgg tcgaccggtt tcagccgcgc cttttggtcc ggatggcacc gcttccggaa
     2041 accgctggca aaatacccct ggtttgcctc atcggggatg aagaaaactt gcccctggca
     2101 caagaaagct ttgaactcgt cgtcacggcg ctttcgctgc aaaatctcaa cgatctcccg
     2161 ggcgcgctca ttcagctgcg ccgcgtcttg aggccggatg gtctctttct cggctgcctc
     2221 ctcggaggca caagcctgca tgagttgcgc acagttttgg ccgtggcgga gacggagctt
     2281 tgcggcggaa tcagcccgcg agtcgcacct ttcgccgatg tgcgcgagct gggcgggctc
     2341 ttgcagagag ctggctttgc cctgccggtc gccgacagcg aaactgttac tgttcgttac
     2401 gccagtctgt ttgacctcat ggctgatctg cgtgcgatgg gcgcgaccaa cgcgcttgtc
     2461 gcgcgattgc gcaagccatc ggcaaaaggc ttttttatgc gtgcggcgga agtctatgcg
     2521 cagcggttcg ccgatcccga cggacgcctg cgtgcaacat tcgagatgat cttcctatcg
     2581 ggctgggccc cgcatgagag tcagcaaaag ccgttgccgc caggctcggc caagatgcgg
     2641 ctttcggacg cgctcgcaat tcaaaaggca aatcgaaagt gatccgctaa tcctcggcga
     2701 gctccctggt gacttggacc tctgtccgca aagtctcgat tcccggctca acgggaataa
     2761 caagagtggc ctcctgcgcg ggcgcggcgg cggccctcat ccgaggtgcc atcccaaccg
     2821 gcaccggggc atgaggctgc gaggcgattt ccaaaacccg cccaagcctt atgccaatcg
     2881 ctttgacata gctattggcc ttccgcatcg catcgcgaac ggcatcgccg cgcaaggaat
     2941 cgattttctc ctccttttgc gaatattgat aagcgatgcc gtcaaaacga ttggcgccct
     3001 tatccaacat ttgctgggcc agggtgccag ccttgccgat atccttgatc cgcacagtca
     3061 gcgaattatt tgcgatataa ccgcgcaagg tccgtttggt aatatagcca gtggcatccc
     3121 gaacttcgtc gatcagcctt gcgacgccta gaatattgag cgcccgtcgc agattgtaga
     3181 cgagcgccgt caagctgaac tcggcgcgga ccttgtcgag gccgcgcatg agaaaggcgc
     3241 cggttcacga tgtgcgtctc gcgtctttga tggatataac gtgagtgtgg cgacgatttc
     3301 gaggctcagg gcgtgattat gctctagcca attcagtccg actgttttcg cccgctgttt
     3361 tcgcgttcaa
//
LOCUS       DATMPU010000209         6567 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_3261565,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000209 DATMPU010000000
VERSION     DATMPU010000209.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 6567)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6567)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6567
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3261565"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..777
                     /locus_tag="VNY06_04825"
     CDS             <1..777
                     /locus_tag="VNY06_04825"
                     /inference="COORDINATES: protein motif:HMM:NF024430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3891 family protein"
                     /protein_id="HWX14172.1"
                     /translation="RKDGPGVIAIPQPSHAWLSGQLARAWGNERFAEPDPHEGVCLAA
                     EQHDIAWHSWEMAPALDLETGLPQEFSKVPSKAHIALWRDGVRRATSFGCYPALLVSL
                     HADTIYARYFDFNKATQEDAEAVREFLDEQHRFQTRITASLHASPEVGVQASPDHIKH
                     NQALVAALDWMSLEICWGVTREKKIPGVPVVWGSTEAISLCPRGGLDLIADPWPFREA
                     YLEVKAEGKRLSGRFKTQAELQRALDEAEPVFVIATLHRA"
     gene            848..2658
                     /locus_tag="VNY06_04830"
                     /pseudo
     CDS             848..2658
                     /locus_tag="VNY06_04830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012589398.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
     gene            2777..3457
                     /locus_tag="VNY06_04835"
     CDS             2777..3457
                     /locus_tag="VNY06_04835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014890063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HWX14173.1"
                     /translation="MPAFLKGFLVHCFTASGAALGLAALFCAADGRFTAMFALLGATI
                     VVDAVDGTLARRARVEETVPHIDGAVLDLVVDFLTYVVVPLVALWRSGLLQSPLAVVV
                     CAIVCAASALYFADRRMKTHDLWFRGFPAIWNVLVFYMLVLRPGPLVSMLAVIIAASL
                     MFAPVVFVHPLRVIRLRLVTLMATGAWSAAAIAALDQGLSQADMITKAALIGVGVYFV
                     VLPLFRNI"
     gene            3483..5540
                     /locus_tag="VNY06_04840"
     CDS             3483..5540
                     /locus_tag="VNY06_04840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012591578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HWX14174.1"
                     /translation="MPGNELCQAASPYLLQHAQNPVHWRMWGAHALQEAQALNRPILL
                     SIGYAACHWCHVMAHESFEDEATASVMNALFVNIKVDREERPDIDHIYMSALFALGEQ
                     GGWPLTMFLTPAGEPFWGGTYFPKATRYGRPSFVSVLKTVAEAFRRHPNQIAKNADIL
                     RSELSKSTEPGAGALTLEQIDALAPKIARRIDPLNGGLRGAPKFPNTPILELLWRAGA
                     RIRDASYRDLVKVTLTKMSEGGIYDHLGGGYARYSTDERWFVPHFEKMLYDNAQILEL
                     LALCYHEFGDELFRARASETVGWLKREMTNAQGAFCASLDADSEGVEGKFYVWTFGEI
                     EALLGAENAAFLAKFYDASTSGNWKDEAHGESWIILNRLKAPAPTQDEEARLSALRQK
                     LFVAREKRERPGLDDKVLADWNGLMIAALVNAATLFGERDWISLAARAYDFVATTMQF
                     SGASGERRLAHGWRAGVLVAPGLALDHAAMIRAALALHETRNWEGESGKTRNYLADAI
                     AWAESLEAYHKDPQSRRLNMAAGDASDVILRLAPTADDAIPNAHPVYLSALVRLSGLT
                     GDPRWLARADALFESVGASVQSNFLSHAGILNALDFRLRAKEIVTAGPRRTALYDTAL
                     GLPFTSRIVLDIEGEEDFPLSHPARAQANLAGEAAAFVCSNETCSLPLRDKETLRDKI
                     GYV"
     gene            5608..5844
                     /locus_tag="VNY06_04845"
     CDS             5608..5844
                     /locus_tag="VNY06_04845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX14175.1"
                     /translation="MLDAISSVVGMFVNILGYASHIITVIFRNLLLIGFIVAVLWGLF
                     TLSRRGYRYLRGMRGEELEHGKPRPKGKPEDSSV"
     gene            complement(6104..6415)
                     /locus_tag="VNY06_04850"
     CDS             complement(6104..6415)
                     /locus_tag="VNY06_04850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX14176.1"
                     /translation="MARFSRGSDWRRLFWMGSLILLSVAFSLGFSCAVPLAPFATIGA
                     LTVLIGAVWLANQMTGFALLHYPLERTAFAWAWFSALSRFLPRLPHNGSTAAFRGGIA
                     S"
BASE COUNT         1358 a         1858 c         1940 g         1411 t
ORIGIN      
        1 cgcaaggacg gcccaggcgt tatcgccatt ccccaaccga gccacgcctg gctttccggt
       61 cagttggcgc gagcgtgggg aaacgaacgc tttgccgagc cagacccgca tgagggtgta
      121 tgccttgcgg ccgagcagca cgatattgcg tggcactcct gggagatggc gcctgcactc
      181 gacctcgaaa ccgggctccc acaagaattt tctaaagtgc cgtcaaaggc tcatattgcc
      241 ctctggcgcg acggtgtgcg ccgagcgacg agcttcgggt gttaccccgc gcttctcgtc
      301 tcgcttcacg cggatacaat ctacgcgcgt tatttcgatt tcaacaaggc tacgcaggag
      361 gatgcggagg cggtgcgtga gtttctcgac gaacagcatc gtttccagac tcggataacg
      421 gcgtcccttc atgccagccc agaggtcggc gttcaggcat cgccagatca cattaagcac
      481 aaccaagctt tggtcgcggc acttgattgg atgtcgcttg aaatctgctg gggcgtcaca
      541 cgagagaaga aaatccccgg ggtaccggtc gtatggggta gcacagaagc catcagcctc
      601 tgcccccgcg gcggccttga ccttatcgcc gatccctggc cctttcgcga ggcttacctc
      661 gaggttaagg ccgagggcaa acgcctgagc gggcgcttta agacgcaagc cgagcttcaa
      721 cgggccttag acgaggccga accggtcttc gtaatcgcca cgctgcatcg agcttgatcg
      781 gcgatattgt gtgccgcgcc agaagccgta caaggagctt aggaactgtt cccatgaaac
      841 cggtcctatg gcgccccctc ttctgctgct gcaaaatatt tcgctgaccc tcggcggcaa
      901 gcctctcttg aacgacgcct cgctttcggt ctcgccggga gaaaaactat gccttgtggg
      961 tcgaaatgga tccggcaaat cgatgctcct aaagatcgcc gcgggtctct tggaaccaga
     1021 tggcggaata tgttttaccc agcctgggac aaagatatgt tatttggcgc aggagcccga
     1081 tctttccctc tattcgacca cgcgaggcta tgtcgaatcg gggcttcgcc ctggcgatgg
     1141 cttacaccgc gccgcagcgc ttctaaaaca attgggactg agcggtgagg aggcgccggc
     1201 gcggctctcc ggcggcgaag cgcgcagagc ggcaatcgtc cgcgcgctcg caccagaacc
     1261 agatgttttg ctcctcgacg agccgaccaa tcacctcgac ctgccggcga tcgaatggct
     1321 cgaagccgaa ctcgaagcct gccagtcggc gcttgtcatc gtcagccacg accgcatgtt
     1381 tctgaataat cttgtggaga ggacggtctg gctcgatcgc ggccgcaccc gcctgctcgg
     1441 gcgtgggttt ccagagtttg aggcgtggcg cgattctttg ctggaagagg aagaacgcgc
     1501 cgagcataag ttcgatcgca aaatcgccgc ggaggaacat tggctgcgct atggcgttac
     1561 cgcgcgccgc aagcgcaacc agaagcggct cgaaggcctt gccgcaatgc gcgcagagca
     1621 gcgccagcgg cttgcttccg ccaaactcaa actcgaggca gcggaagccg atgtttccgc
     1681 cacgctcgtg attgaagcca agcgggtaac aaagtctttt ggcgaacgtg caatcgtcga
     1741 aaatttttcg acccgcattc tgcgcgggga tcggcttggc ttggtaggcg ccaatggcgc
     1801 cggcaaaacg actttgatcc gccttttgac aaaaaccgtt gccccggatc agggctttgt
     1861 ccgccacggc gccaatgtct ttatgggggc tctcgaccag gaccgcgcga gcctcggcaa
     1921 tgggacgatt ttgaaggatg cgcttacggg gggcagcagc gatttcatcg aaatcaatgg
     1981 tgaacgcagg catgtgatcg gctatatgaa ggacttttta ttcggcccag aacaggcacg
     2041 cacgccgatt gagcgtctct ctggcggcga gcgcgggcgg ttgatactcg cgcgcgccct
     2101 agcccggcca tccaacctgc tcgtcctcga cgaaccgacc aacgatctcg atctcgaaac
     2161 cctcgacctc cttcaggagc tcctcgcaag ctataaggga acgcttctga tcgtcagcca
     2221 tgaccgggac tttcttgacc gcgtcgcaac ctcggtactc gttgctgagg gagaagggtc
     2281 ctggatcgaa tatgcgggcg gatattcgga tatggttaag caacgcggtg cagggatagg
     2341 cgcgcccacg ccttcatctg cgcgtgtgca aacaaagaaa cccgcaggtg ctcgacagca
     2401 acggcaaaaa acggcggcca aacgccgcct caattttaag gaaaagcttg cgctggcttc
     2461 gctcccgaca cgtatcgagg agttgcattc gctcaaagaa aagctccacg ctcttctttg
     2521 cgatgcagag ctttacgccc gcgatcccac aaaattcgca aaggccacgg ctgctcttgc
     2581 gaaagccaga cggagctcct ggaagccgag gaggaatggc tccggctcga actgctgcag
     2641 gatgagatgg ggggctaatc gcggacctcg gggcggcggg cggcgatcgc tacggcaggg
     2701 gcttgatgtc ggaagttggc tgtgctccaa ttgcctggag ccgaatatcg cttgccgacg
     2761 atcttgcgga taacaaatgc ccgcctttct caagggcttc ctcgtccatt gttttacggc
     2821 tagtggcgcc gcattggggc ttgccgcgct gttttgcgcc gcggacgggc gatttaccgc
     2881 aatgttcgct ttgcttggcg cgaccattgt cgttgacgcc gtggacggca ccttggcccg
     2941 gcgcgcaagg gtcgaggaaa cggtgccgca tatcgatggc gccgttctcg acctcgtcgt
     3001 cgatttcctc acctatgtgg tcgtgccgct tgtcgctttg tggcggtcgg gtctgctaca
     3061 gtcgccgctt gctgttgtcg tttgcgcgat tgtctgtgca gcctcggcgc tttatttcgc
     3121 cgaccggcgc atgaagacgc atgacctttg gtttcgcggc tttccggcaa tttggaatgt
     3181 tcttgtcttt tatatgttgg tactgcggcc ggggcctctt gtcagcatgc tcgccgtcat
     3241 cattgccgcc agcctgatgt ttgcgccggt ggtctttgtc catccgttgc gcgtcatccg
     3301 cctgcgcctc gtgacattaa tggcgacggg cgcgtggagt gctgcagcca tcgcggcact
     3361 cgatcagggg ttatcgcagg cggatatgat cacgaaggct gcgcttatcg gggttggcgt
     3421 gtattttgtg gtcttgccgc tgtttcgtaa tatttgaccc agtaacccgc caccgtgatc
     3481 ccatgccagg aaacgaactt tgccaagccg ctagccccta tctcttgcag catgcgcaaa
     3541 accccgtgca ttggcgaatg tggggcgctc atgcgctgca ggaagcgcaa gcgctcaata
     3601 ggccaatatt gctctcgatc ggatatgctg cctgccattg gtgccatgtg atggcgcatg
     3661 aaagttttga ggacgaagca accgcctcgg tgatgaacgc gctcttcgtg aacataaagg
     3721 tcgatcgcga ggagcggccc gatatcgatc atatctatat gtcggcgcta ttcgctcttg
     3781 gtgagcaagg cggctggccc ttgacgatgt tcttgacgcc ggcaggtgag ccattttggg
     3841 gcggaactta ttttcccaaa gcaacgcgct atggcaggcc gtcctttgtc agcgtgttga
     3901 aaaccgttgc cgaggccttt cgtcgccatc ccaaccaaat cgcgaagaac gcagacatcc
     3961 tccgcagcga actttcgaaa tccaccgagc ctggtgctgg cgcgcttacc ctggaacaga
     4021 ttgacgccct cgcgccaaag atcgctcgcc gcatcgatcc tttaaacggt ggcctgcgcg
     4081 gcgcgcccaa atttccgaat acacccattc tcgaattgct ttggcgtgcg ggtgcacgaa
     4141 ttcgagacgc ttcctaccgc gaccttgtca aggtgacgct gacaaaaatg tcggaaggtg
     4201 gaatctatga tcatctcggc ggcggttatg cgcgttattc gaccgacgag cgctggtttg
     4261 tgccgcattt tgaaaaaatg ctctatgaca atgcgcagat tctcgaactt ttagcgctct
     4321 gttatcacga gttcggtgat gagctttttc gtgcccgtgc atcagaaacc gtaggctggc
     4381 tcaagcgcga aatgacaaat gcacaaggcg ctttttgcgc cagccttgac gccgacagcg
     4441 aaggcgttga aggcaaattc tatgtctgga cttttggcga aatcgaagcc ttgctcgggg
     4501 ccgagaacgc ggcgtttctt gccaagtttt acgatgcctc gacgagcggc aattggaaag
     4561 atgaggcgca tggcgaaagc tggatcatcc taaatcgcct gaaggcaccg gcaccaacgc
     4621 aagacgagga agcgcggctt agtgccctcc gccaaaaact ctttgtcgcg cgcgaaaagc
     4681 gtgagcgccc aggcctcgac gacaaggttc ttgccgattg gaatggattg atgatcgccg
     4741 cgctcgtcaa cgccgcgacg ctttttggcg agcgcgactg gatctctctt gccgcgcgcg
     4801 cttacgattt tgttgcaacg acgatgcaat tttcgggcgc ttcgggagag cggaggctcg
     4861 cacatggttg gcgcgcgggt gtcctcgtcg cgcccggcct cgcccttgac catgcggcga
     4921 tgataagggc tgcgcttgcc ctgcatgaga cgcgcaattg ggagggggaa tccggaaaaa
     4981 cgcgcaacta tcttgcggac gcgatcgcct gggcggaatc gcttgaagcc taccacaaag
     5041 atccacagag caggcgactc aacatggcgg caggtgatgc gagcgatgtg atcttaaggc
     5101 tcgcgccaac cgcggatgat gcaattccca atgcccaccc ggtctatttg tctgcgctgg
     5161 ttcggctctc agggctaacc ggcgatccgc gctggctggc gcgggcagat gcgttatttg
     5221 agtcggtcgg cgcctcggtg caaagcaatt ttcttagcca cgcggggata ttgaacgcgc
     5281 tcgattttcg cctgagggcc aaggagattg tgaccgccgg gccaaggcga actgcgcttt
     5341 acgataccgc gcttgggctg ccttttacca gccggatcgt gcttgatatc gaaggggagg
     5401 aagactttcc actcagtcat ccagcgagag cgcaagctaa cctcgcaggc gaggcggcag
     5461 cttttgtttg ctcgaatgag acctgctctt tgcccctgcg cgataaagag acattgcgag
     5521 acaaaatcgg ttatgtttag ctgcccgtga gaaacgttca cccaatccag caggaattgg
     5581 gtggccaggg atggagcttg tgcttagatg cttgatgcca tcagttctgt ggtcggtatg
     5641 tttgtgaata tccttggata tgcctcacat attatcactg tgatatttcg taatctttta
     5701 cttattggat ttattgtcgc ggttttgtgg ggattgttca cactcagccg gcggggttac
     5761 cgctatcttc gtggcatgcg cggcgaggaa ctggagcacg gcaaaccccg tccgaagggc
     5821 aaacccgaag acagctcagt ctgagatgtt catgttcgat cgagctgcaa tatcagactc
     5881 gctgttcgcc cgcggaaagc agggtttcct ttttattctc aagacctctg acagcgataa
     5941 gtcggtgcac aagcaaaagc accacaaagg caacggcatt gatgaggaat atacgcagga
     6001 cgatcgctgg agcaattcga gacgccgttt gcgctcgccg tggaaatcgc aaaaagcgcg
     6061 ccttcgtaag ccgcgaaggc cgcaagaaac gtggcgcttg agcctaggac gcgattcctc
     6121 cccgaaaggc ggccgttgac ccattgtgcg gcaagcgtgg caagaaccgc gacagcgccg
     6181 agaaccaagc ccaagcaaag gccgtccgct ccagcgggta atggagcaat gcgaaaccgg
     6241 tcatttgatt ggcaagccac actgcgccga taagaacggt cagcgccccg atggttgcga
     6301 aaggggcaag tggcaccgcg caggaaaagc caagactgaa ggcaacgctt agcaatatga
     6361 gcgagcccat ccagaacagg cggcgccaat cggaaccacg cgagaaccgc gccatttttt
     6421 ctccttatcc tgccagccag gcgatttcag cattttcccg ggccccaggt caagccggaa
     6481 agattttgtc atagccgaac gctgatgatg gtttgtcgga cagaaaacgg gaaagcgcgt
     6541 aattggtctg gaacaatttt ccagtaa
//
LOCUS       DATMPU010000210         7231 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_3261798,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000210 DATMPU010000000
VERSION     DATMPU010000210.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 7231)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7231)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7231
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3261798"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..688
                     /locus_tag="VNY06_04855"
     CDS             <1..688
                     /locus_tag="VNY06_04855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020177210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HWX14177.1"
                     /translation="EQKLDPAELRTRFAGSGLIECGDAHGAGQLTVTNNLVTTAAHVF
                     FNEKGVPRAKTCFFSMDRHGKEVKVPIDLGSIVAGSTNPYAIAAVHDWAVARLIHPVE
                     GVTPYELADSATRNMPVEFVARGHIDWGDGRKLSMEKCRLRDQLTAGEEGTREFSFDC
                     ETGDGASGGALVMGSAKPALGAILVGWRSNKPFHSAPFSHSHYNFAVTVEGAFRKAVI
                     SAASNVAAAK"
     gene            complement(946..1929)
                     /locus_tag="VNY06_04860"
     CDS             complement(946..1929)
                     /locus_tag="VNY06_04860"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012591487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HWX14178.1"
                     /translation="MIQSHILGTGAYAPKQILTNQDLEQMVDTTDAWICERTGIKERR
                     IAAEGEVTSDMAVVAARHAMAMADTNASDLDIIIAGTISADMPLPSCAVVIQAKLGAA
                     HAFAFDVSAACAGSLYALAIADQFIRTGRAQRVLVIGAELLSRLVDWSDRNTCVLFGD
                     AAGAMVIGPANDPQRGLLSTHLHSDGRAAGILSIPGGGSQYPPSEEMLEQKMHKIAMN
                     GREIYKFAVRVLPEAILEALDANGLSVADIDHIVPHQANARIVEKVLEKLGIPLEKCW
                     LNLERYGNTSSASLPISLDEANRAGRLKPGDLIAMMAIGAGMTWGSAVMRW"
     gene            complement(2900..3385)
                     /locus_tag="VNY06_04865"
     CDS             complement(2900..3385)
                     /locus_tag="VNY06_04865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012332101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HWX14179.1"
                     /translation="MAAFCAMVNGTACADPIGSVSGLPIPRYVSLKSDRVNLREGPSK
                     DHRTVWIFQRAGLPVEITAEFEVWRKVRDSEGTEGWVLHSLLSGRRTALVAPWRKGTE
                     SVLYRKASLASAPVAKLQPNVIASVKSCDGAWCRVSGYGFDGFIQQSDLWGVYPNEKI
                     E"
     gene            3870..4865
                     /locus_tag="VNY06_04870"
     CDS             3870..4865
                     /locus_tag="VNY06_04870"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010683391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008465 - glycerate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycerate dehydrogenase"
                     /protein_id="HWX14180.1"
                     /translation="MGRKKPLVIVTRKLPDAVETRMCELFDARLNIDDKPMPRSGLAE
                     ALRTAEVLVPTVTDRVDAELIEVAGPQMKLIANFGNGVDNIDVACAVEHGMTVTNTPG
                     VLTEDTADMTMALILAVARRLVEGAQVIPLGEWAGWAPTWMLGRRIRGKRLGIVGMGR
                     IGQALAHRAKAFGLQIHYHNRRPIPTGIEEALEATYWESLDQMLARMDIISINCPHTP
                     ATYHLLSARRLKYLRPHAIVINSARGEIIDEQALTRMLEAGELAGAGLDVFEHEPAVS
                     QKLVRLAKAGKVTLLPHMGSATAEGRIAMGEKVIINIKSFTDGHCPPDMVLPAML"
     gene            complement(4890..6935)
                     /locus_tag="VNY06_04875"
     CDS             complement(4890..6935)
                     /locus_tag="VNY06_04875"
                     /inference="COORDINATES: protein motif:HMM:NF017262.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopD family protein"
                     /protein_id="HWX14181.1"
                     /translation="MFDPFAIIRAIATLAAAIIVGSSTLLWYSRDALQPAGGAIWRHR
                     IVLTVLVSAFVGAGATWAGVVGSAAAAAGVSAFAIDGSVIATFLTKTGVGKIAIIEIA
                     CAMAACIPTLIAWLTVKKSSTSDLALVCAAVIAGLGLVTYPFNSHPVTLDQQIAGLLS
                     SIAHRLALAVWLGGLPALLLLIGAGPVADDTRQLAAVVLRRFSRLATVAMGVILASGA
                     LLTLFLVRNFPSLIGTQYGYLLIVKLALLGGVLATAQGLQRKLLPMLEVKPADSIFRS
                     YASRVKFETFLALLIVIVASDMAGLAPPEHENIYWPLPFRFSLGATWPVPWVPTEVIC
                     GALLILAGLAVLAFQIVPSFKPKWFRLGANASLAAGIATMLAGSALAFPAISVQAYPD
                     TYLTTDIPFATTSVAAGLKHYEENCTPCHGVSGHGDGPLASSMPVSQKPADLSAPHTA
                     LHTAGDLYWWITHGMSGTPMPGFHDALTDDERWDIINFLGAFAVGYQARSIEPRIFPG
                     QYWLGPPDFQITDETGTARLLSDYQAKSAFLLVLLSCSEDSIGQDRARLEQLLGARER
                     LGALGAKIILVAPGKICEPLRELAAGKILVADRDTADVAATYGLYTRSFHNRKMDVVR
                     VPELHAEFLIDRSGYIRARWLPEEDSSWSDLGFVESQLELLSREPLHPPPPDIHAQH"
     gene            complement(6947..>7231)
                     /locus_tag="VNY06_04880"
     CDS             complement(6947..>7231)
                     /locus_tag="VNY06_04880"
                     /inference="COORDINATES: protein motif:HMM:NF016149.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper resistance CopC family protein"
                     /protein_id="HWX14182.1"
                     /translation="KSAPAARAVLDAAPKELKLWFNEAVEPAFAKVWIIPAEGPQIPL
                     TSHGDSSDRKLLIVAIPDNVPAGAVVIGYHVLSVDGHTIEDKLSFTIKSK"
BASE COUNT         1670 a         2026 c         2086 g         1449 t
ORIGIN      
        1 ggagcaaaaa ctagatccag cagagcttcg tacccgtttc gccggatcag gtcttatcga
       61 atgcggcgac gcgcatggcg cgggccagct cacagtcacc aataaccttg tgaccacagc
      121 ggcacatgtt ttctttaacg aaaaaggggt tccgcgcgca aagacctgct tcttttcgat
      181 ggacaggcac ggcaaagagg taaaggtgcc aatcgacctt ggctcgattg ttgcgggtag
      241 caccaatccg tatgcgatcg ccgcggtcca tgattgggct gtcgcacgtc tcatccatcc
      301 tgttgaaggg gtaacccctt atgaactcgc cgatagcgcg acccggaata tgcccgtgga
      361 atttgtcgcc cgagggcaca tcgactgggg cgatggacgc aagctgtcga tggagaaatg
      421 ccgccttcgt gatcagctga cggcaggcga agaaggaaca agggaattct ctttcgattg
      481 cgaaacagga gacggagcct ctggcggcgc gcttgtcatg gggtcggcca aaccggctct
      541 aggcgcaata ctcgttggct ggcgcagcaa caagccattt cactcggcgc ctttttccca
      601 ttcgcattat aatttcgccg tgaccgtcga gggagcattt agaaaagccg ttatttccgc
      661 ggcaagcaat gtcgccgctg ccaaatagtc gcgcaggaaa ggattttgtg cggaacggct
      721 cgcaaatctg ggagtgcctt gacttctgag gttgtgcgaa gccgacggct cctcacgacg
      781 tccagacttt agcaaagcca aaaccgttgg catgccccgc gcctcacacg cactgcagga
      841 tttacctcaa atgggtgatc aagcgtgccc gagaccacgc ggtggttttc gacttcggcg
      901 ctgaaaattc cattgcatgg gcagcaggcg agaatcaaaa ccggcctacc atctcataac
      961 ggcgctaccc catgtcatcc ccgcgccgat cgccatcatt gcgataaggt cgcccggctt
     1021 aaggcggccg gcgcggtttg cctcatcaag agagatgggc agcgaagcgc tcgacgtgtt
     1081 gccataacgt tcgagattta accagcattt ttcgagcggt attccgagct tttcgaggac
     1141 cttttcgact attcgggcgt tcgcttgatg tggtacaata tggtcaatgt cggcgacgct
     1201 cagcccgttc gcatcgagcg cttcgaggat cgcttccggc agcacgcgaa cggcgaattt
     1261 gtatatctcg cggccattca ttgcgatttt gtgcatcttc tgctcgagca tttcctcgct
     1321 cggaggatac tggctgccgc cgcccggtat cgacagtatt ccggcggccc gcccgtcgga
     1381 atgaagatgc gtcgacagaa gtccacgttg tggatcgttg gcgggtccaa taaccattgc
     1441 ccctgccgca tcgccgaaaa gcacgcaggt gttgcgatcg ctccagtcga caagacggct
     1501 caaaagttcg gcgccgatta cgagcacccg ctgcgctctc ccggtccgaa tgaattgatc
     1561 ggcgatggct agggcataaa gtgagcccgc acatgcagcg gatacatcga aggcaaaagc
     1621 gtgcgccgcg ccgagcttgg cttgaataac aaccgcgcaa gagggtaggg gcatatcggc
     1681 cgagatcgtg ccagcaataa taatatcgag atccgacgcg ttcgtgtcgg ccatcgccat
     1741 cgcgtgtcgc gccgcgacca cggccatgtc cgaagtgact tcgccttctg ccgcgattcg
     1801 gcgttccttg atacccgtcc gctcgcagat ccaggcatcg gtcgtgtcga ccatttgctc
     1861 gagatcttga ttggtcaaaa tctgcttagg agcgtaggcg ccagtcccga gaatgtgcga
     1921 ttgtatcaag aacagggccc ttaattaccg cagcaatgga gctcgaaagg aggttcagca
     1981 ctgccagaac aactcgataa gcccaaactg cgcatttcgc cagcggggaa cggcccagct
     2041 tctccaagaa gctggggggc aattatcaac actaggtaac attttgaaat agtgcgctcc
     2101 aaggtgtcgg agatttcagg agagtgagtc aagccgcgct ggtcgccagg tgatattgcc
     2161 gagctgcgcg agccccagga ttggcaacgt gctaatcggt gtccgccatc gaagggcgcc
     2221 gcacggaaca ttgctgccta tatcctcagg ggaaaaggcg gcaaaaaagc taccgttttc
     2281 tcgcacgggc cacatttcga atcctgaggt gcctaataaa gaaataaagc gcgcgcttga
     2341 ccgaaagggg aagcaaaagc taaagtaact gcgcttaggg agcgaatcgt ttttgggtaa
     2401 caaggggctt gaatgcgcat gtttttcggg atcgtactgg ggatagtttt aacgatcggc
     2461 ggggcgtata tctacgattc gatccgcaac acgagcggtg gcgaaggaac cttcgatcgt
     2521 ccggtagtaa actgggacgt gctcggccac agtgtcaaat cggtaacgtc cgcggttcag
     2581 gacgggttgg cgcgattgac cgggcgacca aaagacaact agtctagcca gcttagcctc
     2641 tgaacttcgt ttcaatcgcc tagcgagaat ccggggcgag cttacgctgc ggcatccacg
     2701 gaagcctcgg cgcttttgca gtgataacgg acaagctccg ccgccctttt acccggcggc
     2761 gattcctgtg gcgctgttcc gctacggcca aaacggttgg cggcgcacac atatagaatc
     2821 tagaatccct ttttggcttc ttgcatccga aaacgtgcct cggcgctaaa tgccgctctc
     2881 gtcgctcgcg aacgcccggc tactcgatct tttcattcgg atagacgccc cagagatcag
     2941 attgttggat aaagccatcg aatccgtagc cggagacgcg gcaccaggcg ccgtcgcagc
     3001 ttttcaccga tgcgattaca tttggctgta atttggcgac gggcgccgaa gccaggcttg
     3061 ctttgcgata aaggaccgac tccgtccctt tcctccaagg cgcaaccaga gccgtgcgtc
     3121 tcccggaaag caaagaatga agcacccacc cctcagtccc ttccgaatcg cggactttgc
     3181 gccaaacttc gaattcggca gtaatctcaa cgggaagtcc agcacgctga aagatccaga
     3241 ctgtacgatg gtccttggag ggcccttcgc gcagatttac ccggtcggat ttcaagctca
     3301 cataacgagg aattggcagg ccgctcacgg aacctattgg atccgcacac gcagtgccat
     3361 tgaccatcgc acagaacgcg gccatgacaa gaccagcgcg ataacaaacc tttttccagt
     3421 gaagccgcat gtggtgcaat acgaggagca cggttcttct catgatcggg tgagattttt
     3481 tgtcgtggtg ttgcgaagcc ctatgcgctg gtgagcagac atcggctacg ctcctaattc
     3541 gctatcacga accgcgccct ctctttagaa taaggcggcc acgaacctgc cttatcggcg
     3601 tgggttagca gatcaaagga ggcccgtccg catccttgtc tttgagcagc cttctgttac
     3661 agatgccaaa ggaaacaatc atgcctctca atacgctcct tcatggacca agcgccgtta
     3721 acgcgaagtg aagactggta acacagtggc tcacatcggc tagtcataaa tctgctgaaa
     3781 gaattggaaa atgtggacgc gaagttaatt ggcatacacc aggggtctgc tgttgggatt
     3841 gccatgagcg tgcacggaca gggattagga tgggccggaa aaagccgctc gttatcgtga
     3901 ctcgcaagct gccggacgcc gttgaaacac gaatgtgcga actctttgat gcgcggctga
     3961 atatcgacga taagccaatg ccgcgcagcg gcctcgccga agcgttgcgt actgccgagg
     4021 tgcttgtccc gacagtcaca gacagggttg atgccgagct aatcgaggtg gcggggccgc
     4081 agatgaagct catcgcaaat tttggcaatg gtgtcgacaa tatcgacgtg gcttgtgccg
     4141 tggaacacgg gatgaccgtc accaacacgc cgggcgttct cacggaggat accgccgaca
     4201 tgacgatggc gttgattctc gcggtcgcgc ggcggcttgt cgaaggggca caagtcatac
     4261 cgctgggcga atgggcaggg tgggcgccaa cctggatgct tggccgccgg atccgcggca
     4321 agcggctcgg aatcgtgggc atgggtcgta ttgggcaagc gctcgcccac cgtgccaagg
     4381 cttttggact acagattcac tatcataaca ggcgccccat tcctacgggc atagaggaag
     4441 cgctcgaagc gacctattgg gaatcccttg accagatgct tgcccgaatg gacatcattt
     4501 cgatcaattg tccgcatacg cccgcgacct atcacttgct ttccgctcgt cggctcaaat
     4561 atttgcggcc gcacgccatc gttatcaatt cggcacgcgg agaaattatc gatgagcagg
     4621 ctctgacccg catgcttgaa gcgggcgagc tggcgggcgc cgggcttgac gtattcgaac
     4681 atgagcccgc tgtctcacag aaactggtcc gtctcgccaa ggcgggcaaa gttacgttgc
     4741 tgccgcatat gggatcggcc actgcagagg gccgcatcgc aatgggcgag aaggttatca
     4801 tcaacattaa gagtttcacg gacggccact gtccgcccga catggtgctg cccgcaatgc
     4861 tctaatcgga gcgggcaggc agcaaaccct caatgttgcg cgtgaatgtc cggaggcggc
     4921 gggtgcagcg gttcacgaga cagcaattcg agttgggact caacgaaacc aagatcactc
     4981 cagctgctgt cttcctcagg cagccagcgc gcgcggatat agccggaacg gtcgatcaag
     5041 aactcggcgt gcagttctgg aaccctgacc acgtccattt tccggttgtg aaagctgcga
     5101 gtatacaagc cataagtcgc ggccacgtcc gcagtatcgc gatctgcgac aaggatttta
     5161 ccggctgcca gctcgcgcag cggctcgcaa atcttgcccg gagcaaccaa gatgatcttc
     5221 gcgccaagcg ctcccagcct ctcccgggca cccaatagct gttcgaggcg cgctctgtct
     5281 tgcccgatgc tgtcttccga gcaggagaga agcaccaata ggaatgcgga cttggcctga
     5341 taatcgctta agagcctggc ggtgccggtt tcatcagtga tttggaaatc cggcggcccc
     5401 agccagtatt gacccgggaa aatccttggt tcgatgctcc gggcctgata gccaacggca
     5461 aaagcgccga gaaaattgat gatatcccag cgctcgtcat cagtaagcgc gtcgtggaag
     5521 cccggcatag gggtcccgga catgccgtgg gttatccacc aataaagatc tccggccgta
     5581 tgcaaggcgg tatgtggggc agacagatcg gctggcttct gactgactgg catgctcgat
     5641 gccaaggggc cgtcgccatg gccgctcact ccgtggcaag gagtgcagtt ttcctcgtaa
     5701 tgttttagac ccgcggcaac ggaagttgtc gcgaacggaa tgtcggttgt gagataagtg
     5761 tccggatagg cttgcaccga aattgctggg aaggcgaggg cgcttcctgc cagcatggtg
     5821 gcaatgcctg cggcaaggct ggcattggct cccaagcgga accacttcgg cttgaaagac
     5881 ggcacaattt ggaaagcgag caccgcaagg cctgccaaaa tcaacaaagc gccgcagatg
     5941 acctcggtgg ggacccaagg aaccggccaa gttgctccga gcgaaaagcg gaacggcagc
     6001 ggccaataaa tattctcgtg ttctggggga gccaaacccg ccatatccga ggctacgata
     6061 acgattagaa gcgctagaaa agtctcgaac ttgacccggc tggcatagct tcgaaaaatc
     6121 gaatctgctg gcttcacctc aagcattggc aagagctttc gctgcagacc ttgagcagtg
     6181 gcaaggacgc cgcccagaag cgcgagttta acgatcagca aatatccgta ctgggttcca
     6241 atcaaggacg gaaaattgcg aaccaaaaac aacgttagca gagcgccact cgcgagaata
     6301 acgcccatcg cgactgtcgc caaccgcgag aagcggcgca agacaactgc cgccaattgc
     6361 cgtgtgtcat ctgcaaccgg accggctccg atcaataata ggagtgcggg caagccaccg
     6421 agccagacag ccaaggcgag gcgatgggct atcgaggaca gaaggcccgc aatctgctgg
     6481 tcgagtgtaa ccggatggct attgaaaggg taagtaacaa gtccaagccc cgcgatgaca
     6541 gcggcgcaga ccaacgccag atcggatgtc gaagatttct tgaccgtgag ccaggcaatc
     6601 agggtcggga tacaagctgc catggcgcaa gcgatctcga taatcgcgat cttgcccact
     6661 ccggtcttgg tgaggaaggt tgcgatcacc gaaccatcga tcgcgaaagc gctaactccg
     6721 gccgcggccg ccgcagaacc gaccacaccc gcccacgttg cgccggcgcc gacaaaagcg
     6781 gaaactagca cggtgagaac gattcggtgg cgccaaatcg ctcctcccgc cggctggagg
     6841 gcgtcccgcg aataccagag caaagtactg gagcccacaa tgatggccgc tgccaaggtc
     6901 gcgattgctc tgattatggc aaagggatca aacatgagcg tacggcctac ttagacttga
     6961 tcgtgaagct aagcttatct tcgatggtgt gaccatcaac ggacaataca tgatatccaa
     7021 tcacgaccgc cccggctggc acgttgtctg gaatcgccac aatgagcaac ttgcggtcgg
     7081 aactatcgcc gtggctggtc aaaggaattt gcggtccctc agctgggata atccacactt
     7141 tggcaaatgc tggctcgaca gcctcattga accaaagctt taattccttt ggcgccgcgt
     7201 cgaggaccgc gcgcgcggca ggagcggatt t
//
LOCUS       DATMPU010000211         5368 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_788126,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000211 DATMPU010000000
VERSION     DATMPU010000211.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 5368)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5368)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5368
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_788126"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1..577
                     /locus_tag="VNY06_04885"
                     /pseudo
     CDS             1..577
                     /locus_tag="VNY06_04885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013214744.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS5 family transposase"
     gene            complement(1515..4517)
                     /locus_tag="VNY06_04890"
     CDS             complement(1515..4517)
                     /locus_tag="VNY06_04890"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HWX14183.1"
                     /translation="MVGSDATYISVRIYFQDPRFVTMSLDATKSGLASIKTILFGAFG
                     ILILLSIGLLSLKVAENWSVYSSGVKVRAFDHAANRYIIGLYEVLIERLATNNALESA
                     APADASAVAEINKRRKAARDNFEVGLAEVRESDFPNKQALLANLNSALEKADEYRRRA
                     DAALPLAGDARDEELRKNFFPVITASVNAALKVWYSALYATAKFDATLTRLAATKEIG
                     WRMREISGFERANVGSAISAGVPLAPNLLAADAESRSRVDLLWDQLGNLTEDPKTPPA
                     IVEAMARAREEYFKTFRKLADDMRAAGEAAGKYPTTAQQWTDTTNPLIDALMAVMYAA
                     GAASESYAASLTDHAANELAVSLGLLVFSLLVEAGVILVVLRQVTRPLTAMSKAMSEL
                     GKGNFGVILPGLGRKDEIGEMAQAVEDFKIKAIEKARLEAQELLVRDAYLQEQNMRFD
                     AALSNMSQGLVMFEGNARLVICNQRYVQMYSLQSDVVRPGCTLRELVDLRIANGSFSG
                     DPDQYIDNLRAAMVGGRPTNHFVQLDDGRTIAEVNQPMAGGGWVATHEDITEQRQAAA
                     KISHMAMHDALTNLPNRLFFREQIENRLTQIDRDQKFAVLCLDLDDFKSVNDTLGHPF
                     GDKLLRQVGERLRSCLREGDNLARLGGDEFALLQGGITQPTDTASLMTRIIEVISAPF
                     DLEGHQVVIGISIGVAVAPTDAADPDQLLKNADLALYRAKADGRGTYRFFEAEMDARM
                     QARRALELDLRRALMKGEFEVYYQPLVNMKTELICGFEALIRWNHPERGIVSPAEFIP
                     LAEETALVVPIGEWVLRQACEEAAKWPSEICIAVNLSAAQFKTPGLSQIVFNALANSG
                     LAADRLELEITESVLLFNSKSTLDTLHHLRALGVRISMDDFGTGYSSLSYLRSFPFNK
                     IKIDQSFVHDLSTNKDSMAIIRAVVGLGNSLGMVTTGEGVETQEEVDYLKREGCTEAQ
                     GYFFGKARPAKDAAALLGKQAVKIKAIA"
BASE COUNT         1156 a         1541 c         1535 g         1136 t
ORIGIN      
        1 atggggttct ggatcgcatt caccatatgc tctacgtcgc ctgccgcgag aaggcggaac
       61 gagaagccag tcccaccgct gccatcatcg atagccaaag cgtgaaaagc gccgaaaaag
      121 gggggcctgc atcgatgcgc atggctatga tgcgggcaaa aagatcaagg gcaagaagcg
      181 ccatatactg gtagatacgc tgggtttgtt gctccttgcc atcgtgcatt gcgccgacat
      241 tcaagaccgc gatggcggca tcttacttct ggcgacgatg aaaggcctgt ttccattcct
      301 cgagaagctg tttgccgaca gtgcttatca ggggccgtta tttgcgaacg ggcttgccaa
      361 aatcctaccc tgtctcaaag ccgagatcat caaacgatcc gatcaagcca aaggattcgt
      421 tcacttaccc aagcgttgga tcgtcgagcg aaccatcgca tggctcaatc gttgtcgccg
      481 tctcgccaag gattgggaaa atctcaaccg caacgccctc gcgttcttga aactggcatc
      541 catccgcctc atgctgcgaa agctttgtaa cccttgatca agttttgaaa cggactctaa
      601 agccgcaaag acgaaagcca cttcagcttc atgaagctgc gttcatcgac atgaacatct
      661 gtaaatattg gtagaaatga gcgctgtatt attgccaaaa atcggtggtg cgcggaaacc
      721 tgatttgtat tatatagtcg tgataggggc gatcttgccc cactctacag ggaggaatga
      781 cgatggttac gaagatcgcc gtcgccgttg cgcttgtggc ttttctcgct gttcccagcg
      841 catcgtttgc agcgtccaag accaaagcct cgtctagagc agacgtgacc aaatgtgagg
      901 gaggaggatg cacggccgtg aatccggatc gcgatcccga tacgtctctt aggtcacaac
      961 actaccgcag cacccataag aaacacaaaa tccaaccaaa gaattgaccc cttcggcgtt
     1021 ttgaggccgc cgaggaccgc gtttggcggc ctcctttcac caagcatcaa tttacgaact
     1081 ggacttgtcg cgctcacgat ttcgaggtcg aagaaggcgc tctgcggctt tcagatattc
     1141 gaggccgaaa tggggaaggc cacaagtgca accgcgacgg ctatgctgcc ttccatcgct
     1201 cacaatcaaa gtaaaacatc tcttttcgca gccggcatta agcgagccgt gatggcaagc
     1261 ctctcgcgcg agggcatagc cagaagcatc gcccaacaca aacgttcgga gaacgaaggt
     1321 gggcggcgga accccgttaa atcgggggcg agtgtgaggt catcaagggt cggcttcgcg
     1381 tcaaacgcgt cgtttcgggc gcgccgtgac cgcggccgct tgcccccaac gaccggacct
     1441 gtctgccaag gatgagtcgg gccaagaggg gacactgatc ggaaagcgcg ctttcccggc
     1501 attggtcgga ccaatcaagc aatagccttg attttgactg cctgcttgcc caagagagcg
     1561 gccgcgtcct ttgccggtcg agcttttcca aaaaaatacc cctgggcctc agtgcagccc
     1621 tcgcgcttca aatagtcgac ttcctcttgc gtttcgaccc cttctcccgt cgttaccatc
     1681 ccaaggctat tacccagtcc cacaacagcg cggatgatgg ccatggaatc cttgttggtc
     1741 gaaagatcat gaacgaagga ttggtcaatc ttgattttgt tgaaggggaa gcttctcaaa
     1801 tagctcaaag atgaatagcc ggtgccgaaa tcgtccatcg aaatgcgcac gcccagcgcc
     1861 cgcaagtgat gcagcgtgtc aagcgtagat ttgctgttga acaagagaac agactcggtg
     1921 atctccagct caaggcggtc agcggccaaa cccgagttcg caagagcgtt gaatacgatt
     1981 tgggatagtc cgggcgtttt gaactgcgca gccgaaagat tcaccgcaat gcaaatttcg
     2041 ctcggccatt ttgcggcttc ctcacaagcc tgccgaagaa cccattcccc gatcggcaca
     2101 accagcgccg tttcttccgc cagcggaatg aactcagcgg gcgatacaat tccgcgctcg
     2161 gggtgattcc agcgtatcag cgcctcgaag ccgcagatca actcggtttt catgtttacg
     2221 agcggttgat agtagacctc gaattcccct ttcattaatg cgcggcgcaa atcaagctcc
     2281 aaggcacgcc gtgcctgcat gcgagcatcc atttccgcct cgaagaatcg gtaggtgccg
     2341 cggccatccg ccttcgcgcg gtacaaagcc aagtcggcgt tcttgaggag ttggtcggga
     2401 tccgccgcat cggtcggcgc aaccgcgacg ccaatactga ttccgatcac gacctgatgg
     2461 ccttcgagat cgaaaggcgc gctgatcacc tcgatgattc gagtcatgag ggacgctgta
     2521 tcggttggct gcgttatgcc cccttgaaga agggcaaatt catcgccgcc gagacgcgcg
     2581 aggttgtcac cttcgcgcag gcagctgcgc agccgttcgc cgacctggcg caggagtttg
     2641 tcgccaaatg gatgccccaa agtatcattg acacttttga agtcatcgag gtcaaggcaa
     2701 agaacggcga atttctggtc gcgatcaatt tgggtgagac ggttttcgat ttgttcgcga
     2761 aagaacaggc ggttgggcaa attcgtcaat gcgtcgtgca ttgccatgtg cgagattttg
     2821 gctgcggcct gtcgttgctc ggtgatgtcc tcgtgagtcg ccacccaacc gccgcccgcc
     2881 atgggctgat ttacctccgc gatggtccgc ccgtcgtcca gctgtacgaa atggttagtg
     2941 ggccttccgc ctaccatcgc ggctcgcaga ttgtcgatat attggtcggg atctcccgaa
     3001 aagctgccgt tcgcaatccg cagatcgacg agctcgcgca gcgtgcagcc gggcctgacg
     3061 acatccgatt gaaggctgta catttgaacg tatcgctgat tgcagatgac gaggcgcgca
     3121 ttgccctcga acatcacgag gccttgggac atgttggaga gggctgcgtc gaaacgcatg
     3181 ttttgctctt gcaggtacgc atctcttacc aggagctcct gagcctccag gcgggccttc
     3241 tcgatggctt ttattttgaa gtcttcgacc gcctgcgcca tctcgccgat ctcgtccttg
     3301 cgtccgagcc ccgggaggat gacaccgaaa tttcctttgc cgagctcgct catcgccttg
     3361 ctcattgccg tcagcggccg ggtcacctgc cgcaatacga cgagtatgac gcccgcctcc
     3421 acgagcaaac tgaatacaag caaccccaac gagacggcga gctcatttgc cgcgtgatcg
     3481 gtcaggcttg ccgcgtagga ctcgcttgcc gccccggccg catacatgac cgccatcaga
     3541 gcgtcgatca gcgggttggt tgtatcggtc cactgctgcg ccgtcgtcgg atatttcccg
     3601 gccgcttcgc cggcggcgcg catgtcatcg gcgagcttgc ggaaagtctt gaaatactcc
     3661 tcgcgcgcgc gcgccatcgc ctcgacgatg gccggcgggg ttttggggtc ctccgtgaga
     3721 ttaccgagtt ggtcccacaa caagtcgacg cgcgagcggg attcggcgtc ggcagcgaga
     3781 aggttggggg cgagcggaac gccggcgctg atcgccgaac cgacattggc tcgctcgaat
     3841 ccggaaattt cgcgcatccg ccagccgatt tccttcgtgg ccgccagacg cgtcagcgtg
     3901 gcatcgaact tcgcggtcgc gtagagcgcc gaataccaca ccttgagcgc tgcattcacg
     3961 gaagcggtga tgaccggaaa gaaattcttt cgcagttctt cgtccctcgc gtccccagcg
     4021 agcggcaagg cggcgtcggc acgcctgcga tattcgtccg ccttttcgag cgcgctgttg
     4081 agattcgcga gcagggcctg cttgttgggg aaatcgctct ccctgacttc tgcgagcccg
     4141 acctcgaagt tgtccctcgc ggccttgcgg cgcttgttga tttcagccac tgcggacgca
     4201 tcggcaggcg cggcggattc aagggcgttg ttggttgcaa gccgctctat caggacttcg
     4261 tacaggccga tgatgtaacg attggcggca tggtcgaatg cccgcacctt gacgcccgac
     4321 gaatacaccg accagttctc ggcgaccttc aggctcaata atccgatcga cagcaggata
     4381 agaatgccga aggcaccgaa cagtatggtt ttgatcgaag ccaagccgga ctttgttgcg
     4441 tcaagggaca tggtcacaaa cctcgggtcc tggaagtaga tgcggacaga aatataggtg
     4501 gcatctgaac cgaccattaa taatttattg ttggcctgcc cccctgccgt gccgcttcgt
     4561 gaccatcgaa agaagtgacg tcatagaacg ttgcggtcgt atccgtcatc gcctacgcta
     4621 cttgaaatcg attgcttaca tttacgccat tgccagccgc gcaagttaaa gtgcgtcgac
     4681 ggtcttgatc gcttgaaaga tgcggaactt gaccctgtca tccccatccg cccctttacg
     4741 acatgtgacg atgcggaaaa ctattccggg attaaggtta agcggcagca aatgcccgct
     4801 cgcgtgcgcc gaaatttcgt ttcgtgagtg caagcgggcc gcatgagtgc aagcgggccg
     4861 ctggtcatgc ggtcagtgcc gaagggcgca aaagcggagc aatttgcgcc ttcttattga
     4921 tcgcgatcga gtggccggct gattactgtc aacgccggag agaaatgcat cggctcagcg
     4981 gagaaaagat gcatcagaga ttacacgctc agaattgtgg accatcagaa attcagtatc
     5041 gcccgggacc actgcagatc gcgtcgacgg ggtgatcgag tggcggctcg ccgaggtccg
     5101 agatgggtca ccagcggacc acgcagcacg tccgcggcgg cagctttcct cagaagcggc
     5161 caatccggat gtttccgcgc cgttggcggc ccatgtcgtc tgagtgggga attaccagcg
     5221 ggacaggctc aagggccgta gggagggggc gcccagccaa tccccggatc gagtccgggg
     5281 aggccaatga cacgggagag tgggaaagac ctcactgctt ctgcggcgga gcgaactcct
     5341 gcatcgtggc agcgacctcg tcgagcgt
//
LOCUS       DATMPU010000212         2624 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_464914,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000212 DATMPU010000000
VERSION     DATMPU010000212.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 2624)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2624)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2624
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_464914"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..330)
                     /locus_tag="VNY06_04895"
     CDS             complement(<1..330)
                     /locus_tag="VNY06_04895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018114852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HWX14184.1"
                     /translation="MKNLCVVAMLAATLAGGSLESASAEPASKAAIKNVVLVHGAFAD
                     GSGWAAVAKILEQDGYRVSVAQPPETSYADDQKYTKAAIDAMGGPVVLVGHSYGGSII
                     TEAGNNPN"
     gene            complement(746..1495)
                     /locus_tag="VNY06_04900"
     CDS             complement(746..1495)
                     /locus_tag="VNY06_04900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX14185.1"
                     /translation="MHAYEIDDWIAARAILARTLWLRGYPDDAKTVAEQCIAEALQVG
                     HEQSTCWAIAFNLCPVAIWRGDFGQAETLVDILLERSQQVFQHYYEWGLLYRRFLGRA
                     ISALDQMDAVWHCSDVKPGISAQADLFATFDGGFAGPASLARAQADEDLWCAPELLRV
                     WAGRLLFSGAKTGQDTTEAMLLRSLGLAKRQDAKAWELRAATSLAQFYLRSNRSHEVR
                     AVLEPTLKHFEQGHDTRDVQAAMNVLFALPN"
     gene            complement(1528..2358)
                     /locus_tag="VNY06_04905"
     CDS             complement(1528..2358)
                     /locus_tag="VNY06_04905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX14186.1"
                     /translation="MFSGSFTMEDAAFLMARIIRFDEAPDRLTSLLDKSLLVARPEEN
                     TFRFRLLDTTRAYAQDKLEESGEANPQRRRHAQRFLQICLAATPDVQSQSSLRQAMGD
                     VRAALDWSLVRGGDMSLGVDLASAATPVFLRLSLLREHRKHLDLALAHISSGTDTVPP
                     TEAALRSEMALRTAVAQAEHYTEGRELAPETQLLRAREIARNIGDKAHEFKVLWMLYG
                     QAGNVGNYRQALVYAKLFEETARGSTDSTTQFRCNRMLARALGDLGQHTLAQHTSNMR
                     "
     gene            complement(2394..>2624)
                     /locus_tag="VNY06_04910"
     CDS             complement(2394..>2624)
                     /locus_tag="VNY06_04910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX14187.1"
                     /translation="ATKSTTQMHHTSPRFAGGWMASPWQSSWPPGGWRDSARKDLPVH
                     LKIVSRSSRMDDAQLSRVIKRYGPRSTGVTGF"
BASE COUNT          563 a          730 c          755 g          576 t
ORIGIN      
        1 gtttggatta ttgccggcct cggtgatgat ggacccgccg tagctgtggc ccaccagaac
       61 cactggcccg cccatggcgt caattgcggc cttcgtgtac ttttgatcat cggcgtagga
      121 ggtctcgggg ggctgcgcca ccgacaccct gtagccatcc tgctcaagga tcttggcgac
      181 agcggcccaa ccggagccgt cggcaaatgc accgtgcacc aaaacgacat ttttgatggc
      241 ggccttcgat gcgggttccg ctgaggcaga ttccagagat ccacccgcca gcgtggcggc
      301 gagcatggca acaacgcaaa gatttttcat ggcatctctc cagttggcag gttcaatgaa
      361 accgtcgcaa gttcgacggc ctgccttcag cctcccattg ccgtatttgg tctgccagtg
      421 aagggatgtg attttttgtt acgcgcgcga gggagccttc attcaagggg gcctaagggc
      481 ctgattctcc tcaaatgatc cttgtcattc taaaaagctg atctttaatc cgtgaattct
      541 atatagttga gtggcgtagc gtcggttgag gcggaaatgt cagtccagct ccatagtgcg
      601 tttggagagc gcctcaagtt tgggcaattc gaatttgctc ccatcgcgcg aaccctttgg
      661 cggcgtggca aacaggtaaa actgggcagt cgcgcactcg acattttaat cgcgctggat
      721 tctaggcaga ggcgagtgct gcacattagt tgggaagcgc aaacaggacg ttcatcgccg
      781 cttgcacgtc ccgcgtatcg tgcccttgtt cgaaatgttt tagcgtgggt tccaggacag
      841 cgcggacctc gtgactccgg tttgatcgta gataaaattg agcaaggctt gtagcggcac
      901 gaagctccca ggccttggcg tcctgccgtt tcgcaagccc gagcgaacgc agcaacatgg
      961 cttccgtcgt gtcttggccc gtcttggcgc cggaaaagag aagacgaccc gcccataccc
     1021 ttagtagttc gggagcgcac caaaggtcct cgtccgcctg cgcgcgcgct aaagaagccg
     1081 gtcccgcaaa tccgccatcg aaggtggcga agaggtcggc ctgtgctgag attccaggtt
     1141 tgacatcgga gcaatgccaa acggcgtcca tctgatccaa tgcagatatc gctcggccaa
     1201 gaaaccgtcg atacagcaag ccccattcgt aataatgctg gaatacttgt tgcgagcgtt
     1261 caagaagaat atcgacgagg gtttctgcct ggccaaaatc gccgcgccaa atggcgaccg
     1321 gacagagatt gaaagcaatc gcccagcaag ttgattgttc atgtccgact tgcaacgctt
     1381 ccgcgatgca ttgctcagct acggttttgg catcgtctgg ataacctcgc agccagagcg
     1441 tccttgcgag gatggctctt gccgctatcc agtcgtcaat ctcgtacgca tgcaaagaaa
     1501 ctcgagggat tgccgcccga tcggagctta acgcatgttc gacgtgtgct gcgcaagagt
     1561 gtgctgccct aggtcaccta gtgcgcgagc cagcatgcgg ttacagcgaa attgcgttgt
     1621 cgaatccgtc gatccgcgag cagtttcttc gaacaattta gcatacacta atgcctgacg
     1681 ataatttccg acattgccgg cctgcccgta cagcatccaa agtactttga actcatgagc
     1741 tttatcgccg atatttcgag caatttccct cgctctgaga agctgtgttt ccggggctaa
     1801 ctcccgccct tccgtataat gttcagcttg cgccacagcg gtgcgaaggg ccatttcgga
     1861 tcggagcgcc gcttcagtcg ggggcacagt atcggtgcca gaggaaatat gagctaatgc
     1921 aaggtcgaga tgttttctgt gctctctgag gagggagagt ctaagaaaga ccggcgtggc
     1981 agccgaggcc agatcgacac caagagacat atcgccgccg cgtaccagcg accagtctaa
     2041 ggccgcgcgg acatcgccca tcgcctggcg taaagacgat tggctttgga cgtcgggagt
     2101 agcggcaagg catatttgaa ggaacctctg cgcatgacga cgtcgctgag gattagcctc
     2161 gccgctttcc tccagcttgt cttgggcata cgcccgagtg gtatcgagca gacggaagcg
     2221 gaacgtattc tcttcgggtc ttgcgaccag cagtgacttg tccagaagac ttgtaagccg
     2281 atcgggagcc tcgtcgaagc ggattatgcg ggccatcaaa aaggcagcat cctccatcgt
     2341 gaagctgccg ctgaacactg aaaggcacct cagcgccgct tggtcttcgg gcgctagaag
     2401 ccggtaactc cagtcgagcg cggcccgtag cgtttgatga cgcgggagag ctgtgcgtcg
     2461 tccatgcgtg aggatcgaga aacaatcttc aagtgaactg gcaagtcctt gcgcgccgaa
     2521 tcccgccagc cgcccggcgg ccagctcgat tgccaaggcg atgccatcca gccgccggca
     2581 aatctcggcg acgtatggtg catctgtgtc gttgatttgg tagc
//
LOCUS       DATMPU010000213         3600 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_2640309,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000213 DATMPU010000000
VERSION     DATMPU010000213.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 3600)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3600)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3600
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2640309"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(115..190)
                     /locus_tag="VNY06_04915"
     tRNA            complement(115..190)
                     /locus_tag="VNY06_04915"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(155..157),aa:Ala,seq:cgc)
     gene            350..640
                     /locus_tag="VNY06_04920"
     CDS             350..640
                     /locus_tag="VNY06_04920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012592526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HWX14188.1"
                     /translation="MRAILDVLLIVLQLYTYVIIGAAILSWLVAFNVVNRYNDVVRSI
                     WNLVTALTEPLLRPIRGVVPNFGGIDISPIILLLGIFFLERVIWQYIYPNVF"
     gene            650..1024
                     /locus_tag="VNY06_04925"
     CDS             650..1024
                     /locus_tag="VNY06_04925"
                     /inference="COORDINATES: protein motif:HMM:NF002348.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 family protein"
                     /protein_id="HWX14189.1"
                     /translation="MNVPWHLRDNELFLSVRLTPKSSRDEIDGIGLLADGRSILKVRV
                     RAVPENGKANTALLRLLAKTLRVPASAVRLESGASGRLKTLCVQGNAKCLVAGLERLC
                     SGCEQGGPATNTKKGPPAPPEG"
     gene            complement(1025..2463)
                     /locus_tag="VNY06_04930"
                     /pseudo
     CDS             complement(1025..2463)
                     /locus_tag="VNY06_04930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011501449.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            2662..3564
                     /gene="murI"
                     /locus_tag="VNY06_04935"
     CDS             2662..3564
                     /gene="murI"
                     /locus_tag="VNY06_04935"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012589440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HWX14190.1"
                     /translation="MSRAPIILIFDSGLGGLTVFTEVARLLPGARLIYAADDAAFPYG
                     ELEEGALVARVMSVMDRLITRCGPDIVVIACNTASTLALSHLRARFPQIPFVGTVPAI
                     KPAASQSRSGMISVLATPGTVARDYTRELIRTYAAHCDVTLAGSSLLAPLAEAYMQGE
                     AVEDAAIAHEIAPAFVSTGEGRTDCIVLACTHYPLLLGQFERLAPWPVEWIDPAPAIA
                     RRTAEVLREHFGVLAEDGSAAAPPGYAAFTAPEGPGPELRKALHLRGIEKLVTEPIPL
                     AGLRRNQKTPRRTELGDPAGRAAS"
BASE COUNT          804 a         1043 c         1003 g          750 t
ORIGIN      
        1 cgaggctttg aatcctttga tgaaatctat gcccctcttc cgcaggaagg ttgccgcact
       61 tgctaattgg ccggcgccag tttcgttttc taaaacataa cacgttgata ttattggtgg
      121 agctgagggg attcgaaccc ctgacctctg cagtgcgatt gcagcgctct cccaactgag
      181 ctacagcccc ctggcgccgc tctttacgcg acgggcacta ggcatgcaag ccggttcgtg
      241 ccaccgagct taccagtcac acatgtcgag aagagcgcat ccgtgccctt gccagctttg
      301 cacgggccgc gtaaacgagc atagtctgag caagcattct ggagatctga tgcgcgctat
      361 cctcgacgta ctcctgatcg ttctacagct ttacacttac gtgatcattg gggcggcgat
      421 cttatcctgg cttgtcgcct tcaatgtcgt taatcgctac aatgatgtcg tgcgctcgat
      481 atggaacctc gtgactgctt tgacggaacc gctgcttcgg ccgatccggg gcgtcgtgcc
      541 aaattttggc ggcatcgata tttcgccgat tattttgctc ctcgggattt tttttctcga
      601 gcgtgtcatc tggcagtata tctatccaaa cgtgttctag cccctcttgg tgaacgttcc
      661 ttggcatttg cgcgataacg aactgttcct aagcgtccgg ttgacgccca agagttcgcg
      721 cgacgaaatt gatggcatag ggcttcttgc ggacggacgc tccatcctaa aagtgcgcgt
      781 gcgggcagtt cccgaaaatg gcaaggccaa cacggcgctc ctgcgactgc ttgcgaagac
      841 actgcgcgta ccggcgagcg cagtacggct agagtcgggt gcaagcgggc ggttgaaaac
      901 cttgtgcgtt caaggaaacg cgaaatgctt ggtcgcgggt ttggaacgtt tgtgttccgg
      961 ctgcgaacag ggcggcccag ccacaaacac caaaaaagga ccgcctgctc caccggaagg
     1021 ttgattatag agacgacaag gggcgcgcca cctcctcgag gggttttcgc tcggcggcca
     1081 cgccccaaat cgctgcgaca atggcagcta ccaccatcag caaggaacca agcaaatagc
     1141 ctacaaagac actttcgcgc gaaccggact caattagaac cgcgaataaa aatggcgcaa
     1201 cggtcccgcc aatcgcggta ccggccgaat aaaaaaaggc aatcgctagc gcgcgaattt
     1261 cgaccggaaa attctcagat gccgtcagat aggcggcacc cgccgccgct gacgcaaaaa
     1321 agaagatcac tgtccacgcg atcgtttgct cgagcgcgct aagaaggcca tgcataaaca
     1381 gccagccgct taacgcaaga agcacaccgg aggcgacata ggtgagggcg atcatgggct
     1441 ttcggccgat cgaatcaaac agcgggccaa ggaccaatgg accgagcaca ttgccaagcg
     1501 cgaaggggag aatataccag ccgacattat aagaaggcac actataaaat ttcgttagaa
     1561 cgagtgcgta agtgaagaag acggcattat aaaaaaatgc ttgcgtcgcc atgagagtga
     1621 ggcagacaag cgttctcgtt ctgaatgcca cgaagagcgt gtgaaaaacc tcctttaggg
     1681 gcgtgaagcg gcgcgggcgc aatacattgg ctccagtaaa cacgcatcgt caatagacga
     1741 gccgtgcagc cggaaactgg cttcgatttc atcaactatt ttttcggctt ccgcttgccg
     1801 tccatgaatt acaagccagc gcgggctttc cgggatccaa gccctcagta aaataatgaa
     1861 gaggccaagg acggagccga caagaaatgc taaccgccag cctatgtccg gatcaaaccg
     1921 gcctggatcc agaacggcga tcgatgcgac ggcgccaagc gcgccgccga tccagaacga
     1981 gccgttgatg gcaaggtcgg tccaccctcg gtaccgcgcc gggatcattt cttggattgt
     2041 ggaattcacc gcgctgtttt cgccgcctat ccccgccccg gtcaagaacc gaaacagaca
     2101 aaaactcgca agattccagc tgagtgcggt ggcggctgta aataccaaat atacactcaa
     2161 ggttataaaa aacataggcc tgcggccgag ccggtcggtt agccatgcaa agccaagcgc
     2221 gccaatgatt gcgccagaga gataaaggct tgccgccaag ccaatatcgc gcgcggtgaa
     2281 atgcaagcgg gggcttgttt cgagcgcgcc ggcaacagaa ccggcgaggg taacttcgag
     2341 cccgtcaagc acccaggtga ttccgagcgc gataacgatg agggtatgaa atcttcccca
     2401 tggcagccgg tcgaggcggg ccgggatatt ggttttagta atcttgttcc cttggtcgtt
     2461 catggctggc ctcttcgctc gcgaccacgg cgatcgcatc ccctcacttc taacggccaa
     2521 ccctcatacc ctgctttata atcgccgcga caggggctca acggggtatt aaccaaaatc
     2581 tgccttgatc cgctatgata ctatcactct taactccgaa cgcagccttc gaggcaaata
     2641 cctgtctaat tggttccaat catgtctcgc gcgccaataa ttttgatctt cgattccggc
     2701 ctcggcggcc tgacggtttt tacggaggtg gcgcgccttc ttccgggcgc tcgcttgatc
     2761 tacgccgctg acgatgctgc gttcccttat ggtgaacttg aggaaggcgc tttggttgcg
     2821 cgggtcatgt cggtgatgga ccggctgata acgcgctgtg ggccggatat cgttgtcatt
     2881 gcgtgcaata cagcctccac gcttgcgctt tcgcatttgc gcgcgcgctt tccgcaaatt
     2941 cccttcgtcg gcacggtgcc ggcaattaaa ccggccgcaa gccaatcgcg ttcagggatg
     3001 atctcagtgc tggccacacc cggcaccgtt gcgcgcgatt atacgcgcga gctgatacgc
     3061 acttacgccg cgcattgcga tgtgacgctt gccgggtcaa gcctgcttgc gcctctcgcc
     3121 gaagcttaca tgcaaggaga agccgttgag gatgcggcga tagcgcatga aatcgcgccc
     3181 gcattcgttt cgactggcga aggccgcacc gattgcatcg tgcttgcgtg tacgcactat
     3241 cccttgttac ttggtcagtt cgaacgtctt gcgccatggc ccgtggaatg gatcgatccc
     3301 gccccggcca tcgcccgccg gaccgccgaa gtcttgcgcg agcactttgg tgtcctcgcg
     3361 gaagacgggt cagccgcagc gccgccgggc tatgcggcct ttaccgcccc tgagggacca
     3421 ggacctgagc tacggaaagc tttgcacctt cgcgggatcg aaaaactcgt caccgagccg
     3481 atccctctgg ccggactgcg ccgaaatcaa aaaacgccgc gccggacgga gctcggcgat
     3541 ccggcaggcc gggccgcttc ttaacggcta ctttgattgg tatggccggc gcaatccgcc
//
LOCUS       DATMPU010000214         2802 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_466414,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000214 DATMPU010000000
VERSION     DATMPU010000214.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 2802)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2802)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2802
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_466414"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(191..1498)
                     /locus_tag="VNY06_04940"
     CDS             complement(191..1498)
                     /locus_tag="VNY06_04940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020176421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWX14191.1"
                     /translation="MSEIPTVSARPFAMLASLLRSRRFASLFWCQFFSVFNDNYVRNM
                     LAMLILFRLGEDEAGVLITLAVGTFIAPSLFLSGLGGELADAHDKATLARRLKFAEIP
                     VQFLAAIGLVLSSLPLLYAALFGLGVISALFGPVKYGILPDLLEKEELLAGNSLVEAA
                     TFIAIFLGLIAGGLSVTGRTPEGTVLQLIVIAIACFAASLFIPPAGRCPDDLRANANI
                     LVSTVERLGELARDAMLWKSSVAVSWFWMNGAVALSLVPVVVRDKVGGGIVAETAVSA
                     LFALGIGIGSLAAAVIARGRIFLKPVPFAALGMAAFLIDLGAATYFLPVAEAQVGLGE
                     FFRSLIGVRVAADVVGLAGAGGLFVVPLFTAIQADAPKDHRARIVGGVNILSSAFIVL
                     GALTTAMLQSRTIGVNEPALLAALGVLNLGAAYYVRSVSLGTR"
     gene            complement(1495..>2802)
                     /locus_tag="VNY06_04945"
     CDS             complement(1495..>2802)
                     /locus_tag="VNY06_04945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012589249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HWX14192.1"
                     /translation="AAKFATGFASGSLAVLSEAGHNLGDVAATTLTYFAIRIANKPAD
                     EDHQFGHAKVEALAALAETGFLFALAVYILIEAIARLVANDTNIEISLSVYVVLLASI
                     IVDLVRWRALKRIATRTKSDALLADSLHFSSDLVSSALALVGVTAAHFGYPQGDPLAA
                     LGLVAFIAAAGLQIGRRTVNTLVDTAPRGLAPQVKSLARSVPGVVDVETLRLRPAGAE
                     VVGDIGIAVSRTLPLEHAAAIEANVASAISARYPEITVKVAAQPIALDDETILERVLL
                     IAAKRHVPVHHVTIQDVDGRTSISFDAELDGQMRHASAHEIISALETEIGRELGLGIE
                     VETHIEPLEPRPLKGHPVPEEIRVELASVLEGIAASGEIHDVHSVRVRTTPDGLVVNY
                     HCRVSPYLSVGEVHAQVDELDRKMRGAFPGIVRIVGHAEPLRE"
BASE COUNT          671 a          805 c          806 g          520 t
ORIGIN      
        1 aaggccccgc cgttggctag acaatcgtaa tggtcataat caataatcac aatgtttgtc
       61 ttgctggcgg tcccaattcg ggaagttggc tagggtcgac agctaccttt cagaaactcg
      121 cttttatcgt gcccggacta acctgcacgc cttattctga tctcgcatct gctggctgga
      181 agaattgctc tcacctagtc cctagggaaa cagacctgac ataataggcc gcgccgaggt
      241 taagcacgcc aagagcagca aggagcgccg gctcgttgac gccaattgtg cggctttgca
      301 acatggccgt cgtgagcgcc cccaaaacaa taaacgcaga gcttaaaata ttcacgccgc
      361 cgacgatcct ggcgcggtgg tccttgggtg catcggcctg gatcgcggta aacagcggca
      421 cgacaaaaag tcctcccgcg ccagcgagcc ccacaacatc ggcggcgacg cgtaccccaa
      481 taagtgaacg aaagaattcg ccaaggccca cttgtgcttc cgccacgggg agaaagtaag
      541 tggcggcgcc aaggtcgatg agaaaggctg ccatcccgag cgcggcgaaa ggaacgggtt
      601 ttaaaaagat gcgtccgcgc gcgatcaccg ctgctgcaag cgaaccaatc ccgatgccga
      661 gcgcaaagag cgcgctcacc gccgtctcag ctacgatgcc gccgccgact ttgtcgcgca
      721 ctaccacggg cacgagcgaa agcgcaaccg cgccgttcat ccagaaccac gagaccgcca
      781 cgctgctttt ccaaagcatg gcgtctcttg ccagttcacc aagtcgttcg accgttgaca
      841 cgaggatatt agcatttgcg cgaaggtcat cagggcagcg ccccgccgga ggaataaaga
      901 ggctggcggc aaagcaggcg atggcaatga cgataagctg gaggacggtt ccttccggcg
      961 tgcgccctgt gactgaaagg ccgcccgcaa tcaagcccaa gaatatggcg atgaaagtcg
     1021 ccgcttcgac gagtgaattt cccgcgagaa gctcctcttt ctccagcaga tctggaagga
     1081 tgccatattt gacggggccg aacagagccg aaattacgcc gagcccaaac aaagccgcat
     1141 agagcagggg cagcgaggag aggacgagtc ctatcgcggc aagaaactga accggtattt
     1201 ctgcgaattt gagacgccgc gcgagcgtgg ccttgtcatg tgcgtcggcc aattcgccgc
     1261 caagcccgga aagaaacagc gaaggagcga taaatgtgcc aacggcgagc gtgatcaata
     1321 cgccggcttc gtcttcgccg agtctgaaaa ggatcagcat ggcgagcatg ttgcgcacat
     1381 agttatcgtt aaacacggaa aagaactgac accagaataa gcttgcaaag cgacgcgaac
     1441 gcagcaagga ggcgagcatg gcaaagggtc gagccgaaac ggtaggaatt tcgctcattc
     1501 acgcaacggt tcagcgtgac caacgattct gacgatacca ggaaacgctc ctcgcatttt
     1561 ccgatcgagc tcgtcgactt gagcatgcac ttcgccaacg ctaaggtaag ggcttacccg
     1621 gcaatgatag ttgacgacca gaccatccgg cgttgtcctt actcggacgc tgtgcacatc
     1681 gtggatttct ccgcttgccg caatgccctc aaggacggag gcgagctcaa cgcgaatctc
     1741 ctctggcacg gggtgacctt ttaatggccg gggctccaac ggctcgatat gggtttcaac
     1801 ctcgatgccg aggcccaatt cgcggccaat ctccgtttcg agcgcggaga tgatctcgtg
     1861 cgcgctggca tgcctcattt ggccgtcgag ttcggcgtcg aagctgatcg atgtacgccc
     1921 atcgacatcc tgaatagtca cgtggtgaac cggaacatgg cgcttggcag ctatcaaaag
     1981 aacacgttcg agaattgttt cgtcgtcgag tgcaatgggc tgcgcggcaa ccttcacggt
     2041 gatctcggga tagcgggcgg agattgcgga tgcaacattg gcctcaatcg cggcggcatg
     2101 ttcaagcggc agggtgcgcg acacagcgat tccaatgtca ccgacgacct ccgctccagc
     2161 tgggcgcaac cggagcgttt cgacatcgac cactccgggg acagaacgcg cgagtgactt
     2221 tacctgcgga gcaagtcctc ttggcgcagt atcgacgagg gtattgaccg tgcgccggcc
     2281 aatctggagg cctgcggccg cgataaacgc gacgagtccc aatgcggcca gcgggtctcc
     2341 ctgcggatat ccaaaatgtg ccgcggtgac tccaacgagc gccagcgctg agctgacaag
     2401 atcgcttgaa aaatgcagcg agtcagccaa gagcgcatcg cttttcgtcc gcgttgcaat
     2461 ccgcttcagt gccctccaac ggaccaaatc cacgatgatc gacgctagta gaacgacgta
     2521 gaccgataaa ctaatttcga tattggtatc attagcgacg agccgggcga tggcttcgat
     2581 caatatataa acggctagtg cgaacaagaa cccggtttcg gctagggccg caagggcctc
     2641 caccttggca tggccgaatt ggtgatcttc atcagctggc ttgtttgcga tccgtatcgc
     2701 gaaatatgta agagtggtcg ccgccacatc gccaagatta tgaccggcct cggagagcac
     2761 cgcgagcgag cctgaagcaa agcccgtggc gaatttcgcc gc
//
LOCUS       DATMPU010000215         5465 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_1713899,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000215 DATMPU010000000
VERSION     DATMPU010000215.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 5465)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5465)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5465
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1713899"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(260..1759)
                     /locus_tag="VNY06_04950"
     CDS             complement(260..1759)
                     /locus_tag="VNY06_04950"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020176064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006793 - phosphorus metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ppx/GppA family phosphatase"
                     /protein_id="HWX14193.1"
                     /translation="MASGPAQTHGPIAIVDVGSNSVRLVVYECLSRSPRPVFNEKSLC
                     ALGTGVATTGRLPAAGVEKALAALRRFRVLSDVLGVSALHVLATAAARDASNGADFLA
                     AAAKAISAPVVLLSGDREAELSALGVISGFVNPDGIVGDLGGGSLELTDVKGARASKG
                     LSLPLGGLTLMDASGRSPRAATKIVREALANARVAERLAGRTVYAVGGTWRALAKLHM
                     SQRNYPLAVTHGYAIPASEAADFAGLVESANADTLVSISAISAARRPFLAYGALVLDE
                     IIRRARPREVVFSATGMREGLLYEMLAMEQRRQDPLLLAASQFDQLFSRAPGHANELC
                     DWTDGLMKSMRTEESLEERRLRQAACLLADVNWRAHPDHRHEEGMNIAENAAFLGVDH
                     PGRSFLALVASYRYLGIDADVSPQIRALVSARMLEKARILAAAVRAAFVISGAMPGVL
                     PAAPVHCTKTQLILTLPRRFADLATEKLQSRIAQLGRLIRREPAFTIAD"
     gene            complement(1785..3956)
                     /locus_tag="VNY06_04955"
     CDS             complement(1785..3956)
                     /locus_tag="VNY06_04955"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012384557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA degradosome polyphosphate kinase"
                     /protein_id="HWX14194.1"
                     /translation="MMEAGEALVTPSSELTRSPRRFINRELSWLEFNRRVLQEASNRN
                     HPLLEQLRFLSISADNLDEFFMVRVAGLRGQMRAGITAPSEDGLSTAEQLGKIRERAS
                     LLAAEQQRRWRELRKELCEAGVTILDPADLIGAETEWLRQHFLTHVFPVLTPLAVDPA
                     HPFPFIPNLGFSIALELFGPNDQTNRTALIRLPAKIERFIRLPDGLSENGLRFVALET
                     TIGMFTQSLFPGYQVHGHGLFRIIRDSDLEVEEEAEDLVLLFESALKRRRRGSVIRLE
                     VDSLMPDELRSFVAEELDVVSEEIYVLDGMLALNELSELVNLNRPDLKFKTYNPRFPE
                     RVRDNGGDCFLAIKQKDLVVHHPYESFDVVVQFLNQAAADPNVVAIKQTLYRTSSDSP
                     IVRALIDAAEAGKSVTALVELKARFDEAANIRWARDLERAGAQVVFGFIELKTHAKLS
                     MVVRREAGTLTTYCHVGTGNYHPVTAKIYTDISFFTADPVIGRDVSRIFNFITGYAEP
                     AGLERMAVSPMSLKQRLLEHFAEEIEFAKVGKPAAIWAKCNALVDPEIIDALYEASNA
                     GVNIDLIVRGICCLRPGVEGLSENIRVKSIIGRFLEHARVYCFGGGHDLPHPKAHVYI
                     SSADLMPRNLDRRVEALLPILNPTVHEQVLGQIMVANLIDNQQSYQVLPDGSSTRITL
                     PEQEEPFNAHSYFMTNPSLSGRGKSLKTSSPKTLLKRLDGR"
     gene            4440..>5465
                     /gene="ald"
                     /locus_tag="VNY06_04960"
     CDS             4440..>5465
                     /gene="ald"
                     /locus_tag="VNY06_04960"
                     /EC_number="1.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010969355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000286 - alanine dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HWX14195.1"
                     /translation="MRIGIPKEIKDHEYRVGLTPDSVREFAAAGHELSVETGAGAGIN
                     ATDEAYRAAGAVMVGTADEIFANSETIIKVKEPQECEWTKLRENQIIFAFLHLAANSA
                     LTKGLLASGCTAVAYETVTGQGGNGLPLLAPMSEVAGRLAIEAAGLALQHQNGGRGVL
                     LGGVPGVLPARVTILGGGVAGTQAARMAIGLGADVTILDCSLQRLCQIDGLFQGRPKT
                     RFATAAAIEDTVSSSEAVIGAVLVPGATAPKLVTERMLSTMRQGAVLVDISIDQGGCF
                     ESSHATTHSAPMFLKGGIVHYCVANMPGAVPVTSTHALNHAMLPYGLALAEGGLRSLA
                     RNPGLRAG"
BASE COUNT         1231 a         1493 c         1586 g         1155 t
ORIGIN      
        1 ggaccaatcg ctctcgccgc tcagatcaga gagcagttcc aatatcggca cgagatgggg
       61 tcttcttggg caaagatacg tgacgctggc tacgcccggt cactgactcc atcccacagt
      121 ggggatggag ttgtcggtgg ggtcaggcgc tgatcccgat atcttgactt acaggtttga
      181 atcctagcag gcgttggagc cgtatacggt cactgcggcg cggcgacggg ataatgcatc
      241 aatacgccaa acagctaaat taatctgcga tcgtaaaagc aggctcgcgc cggatcagac
      301 ggccaagctg cgctatccgg ctttgcaact tttcggttgc aagatcggca aaccgtcgag
      361 gcagcgttaa aatcaactgg gttttggtac agtggaccgg ggctgctggc agtacgcctg
      421 gcatggctcc agagatgaca aaggcagcgc gtaccgcagc tgcgagtatg cgcgcttttt
      481 cgagcatccg cgcggatacg agagcacgaa tctgcgggct gacgtcggca tcgattccga
      541 gatagcgata cgacgccacc agtgcgagga atgaacggcc cggatgatcg acgccgagga
      601 aagccgcatt ttcagcaata ttcatgcctt cctcgtggcg gtggtcgggg tgagctcgcc
      661 agttgacatc agccaaaagg caggccgcct ggcgaaggcg gcgttcctca agggactcct
      721 cagtacgcat ggatttcatt aacccgtcgg tccaatcgca aagctcattg gcatgtccgg
      781 gagctcgcga gaaaagctga tcaaattgtg atgccgcgag aagtagcgga tcttgccgac
      841 gctgctccat ggcaagcatt tcataaagca ggccttcgcg catacctgtt gccgaaaata
      901 cgacttcgcg cggccgcgcg cgccgtatga tttcgtcaag cacgagcgcg ccgtaggcga
      961 gaaatggtcg ccgcgctgcg ctaatggcac taattgaaac caatgtatcc gcgtttgcgc
     1021 tttcaacgag gccggcgaaa tccgctgcct cggaagccgg gatcgcatag ccatgggtta
     1081 cagcaagcgg ataattacgt tggctcatat gcagcttggc aagcgcgcgc caagtcccac
     1141 caaccgcata gacagtacgt ccagcgagac gttctgcgac acgcgcgttc gccagtgctt
     1201 cgcggacaat tttggttgcc gcgcgtgggg agcggcccga ggcgtccatc agggtcagac
     1261 caccaagcgg caggcttaag cctttgctcg cgcgtgctcc cttcacatcg gtcaattcga
     1321 gcgagccgcc gccgaggtcg ccgacgattc catctggatt gacaaatccg gagatcacac
     1381 ccaaagcgga aagctccgct tccctatcgc ctgataaaag cacgaccggg gcgcttattg
     1441 ctttcgctgc cgcggctaga aagtcggctc cgttcgaagc atctcgggcc gcagcagttg
     1501 cgagaacgtg caaagcggag acgcccaaaa catcacttaa gactcgaaag cggcgcaacg
     1561 ccgctagtgc tttttcgacg ccagcagcag gtagtcgacc ggtggtcgcg acgccggttc
     1621 ccagcgcgca aagagatttc tcattgaaaa ccggccgggg cgagcggctt aaacattcat
     1681 agacaacaag tcgaaccgag tttgagccaa cgtccacgat tgcgatgggc ccatgagtct
     1741 gcgctgggcc agaagccata ttacaatttt cagctatcaa atgtctaacg cccatccaac
     1801 cgcttgagta gggttttggg gctcgaagtc ttgagggatt ttccacggcc ggacagactg
     1861 gggtttgtca tgaagtagga atgcgcattg aatggctctt cctgttcagg cagagtgatg
     1921 cgcgtgctcg atccgtccgg caatacttga tagctctgct ggttatcgat cagattggct
     1981 accatgatct gaccgaggac ctgctcgtgg acggttggat tgagaatcgg aagaagagcc
     2041 tctactcttc ggtcgagatt gcgcggcatg aggtcggcgg aagaaatata gacatgcgcc
     2101 ttggggtgcg gcagatcatg accaccgcca aagcaataga ccctagcatg ttcgagaaac
     2161 cggccgatga tcgacttgac tcttatgttt tcggacaatc cctcgacgcc tggcctaagg
     2221 cagcagatac cgcgcacgat aagatctatg tttactccgg cattgcttgc ctcatataag
     2281 gcgtcgatga tttcagggtc gaccagagca ttgcacttcg cccaaatcgc ggcgggcttg
     2341 cctaccttgg cgaattcgat ctcttccgca aagtgctcga ggagcctctg cttgagcgac
     2401 atgggcgaaa cggccatgcg ttcgagccca gcaggctcag catagccggt aataaaattg
     2461 aatatacgcg acacatctcg cccaattacc gggtcggcag taaagaacga aatgtcagtg
     2521 tagatctttg ctgttactgg atggtaattt ccggtaccga catggcagta ggttgtaaga
     2581 gtaccggcct cacggcgcac aaccatggaa agtttggcgt gcgtcttcag ttcaatgaat
     2641 ccaaacacca cctgtgcccc ggcacgctca agatcacgcg cccagcgaat attggcggcc
     2701 tcgtcgaatc gcgccttcag ttcgacaagg gcggttaccg actttccggc ttccgcggca
     2761 tcgatgaggg cgcggacaat tggactgtcg gatgacgtgc gataaagcgt ttgtttgatt
     2821 gcaacgacat taggatctgc tgctgcctga ttcaggaact gaaccaccac atcaaaggat
     2881 tcataggggt ggtggacgac aaggtccttt tgtttgatcg ctagaaaaca gtcgccgcca
     2941 ttgtctcgga cgcgctctgg gaaacgcgga ttataagtct tgaatttaag gtctggccgg
     3001 ttgagattga caagttcgga cagctcgttc aaggcaagca taccgtcaag gacatatatc
     3061 tcttcgctga caacatcgag ttcctcggca acgaaactgc gcaactcgtc aggcatgaga
     3121 ctgtcgacct cgagccttat caccgaaccc cggcgacggc gcttcagcgc gctctcgaat
     3181 aataatacga ggtcttcagc ttcttcttcg acttcgagat cgctatcgcg aatgatccgg
     3241 aacagaccat gaccgtgtac ctggtagcca gggaaaagac tttgcgtgaa cattccaatc
     3301 gtcgtttcga gcgcgacgaa gcgcaggccg ttctcactaa gaccatcggg gagtctgata
     3361 aaacgctcga tcttggcagg gaggcgaatc agagcagtcc ggttcgtttg gtcgtttggc
     3421 ccaaatagtt cgagagcgat cgaaaagccg agattaggga taaatggaaa cggatgcgcc
     3481 ggatcaacgg caagcggtgt caaaaccgga aagacatgcg tcaagaagtg ctggcgtagc
     3541 cattctgtct cggcgccgat aagatcagct ggatcaagaa ttgtaactcc tgcttcgcat
     3601 aactcctttc gtaattcgcg ccagcggcgc tgctgttccg cggccaaaag gcttgcgcgc
     3661 tcgcggatct ttccgagttg ctcagccgtt gaaagtccgt cctcagacgg agccgtgatt
     3721 ccagcgcgca tttgcccgcg aagtccggca acccgcacca tgaagaattc atcaagatta
     3781 tcggcggaga tcgacaaaaa tcgcaattgt tcgagcagtg gatggttgcg gttcgatgct
     3841 tcctgcaata cgcggcggtt gaattcgagc caagaaagtt ctcggttgat aaaccggcgg
     3901 ggtgagcgag tgagttcgct tgatggcgtg accagtgctt cgccagcctc catcatgaaa
     3961 atcgacgcga ccggcgagag ggcggcctgg gcggcgccga tggactcgga gggctcaatc
     4021 gaagcgctct tcccgccaat gtcgtcaaag ggcgtgcctt tcggctcaaa agtgtccact
     4081 ctcgcgacgc ggcgttttgg tgctggcccg cgcttgccaa cagcgagctt ctgtccttcc
     4141 actgccgaac tcacactgtc ctccgtaaaa acaaatgcca gggaaactac ttcgcaatta
     4201 atgacgggag catgaccgtt cacccccttc accttgcgtt catttttaga caaggcgagc
     4261 gggaaatccg cgattttata gcacgactta acactataag gccggaaggc ccgcaggcag
     4321 ctgcctttcg gttgcctagc tgctacgttg cggggaattg accatttgta ttccagcccg
     4381 gagcctacca catatagcgg aagcgggaat ataaggcact gtcaaatgga gcttaaggcg
     4441 tgcgaattgg tatccctaag gaaatcaagg atcacgaata tagggtgggg ctaacgcccg
     4501 actcggtccg cgaattcgcc gccgccggac atgaactgag cgtcgaaact ggtgcagggg
     4561 ccggtatcaa tgcgacggac gaggcctacc gggccgcagg tgcggtcatg gtcggtacgg
     4621 cggatgaaat ctttgccaat tcagaaacga ttattaaggt gaaggagccg caagagtgcg
     4681 aatggacaaa gttgcgcgag aatcagatca ttttcgcatt tctgcatctc gcggcaaatt
     4741 ctgcactgac aaagggcttg ctggcttcag ggtgcacggc ggtcgcctat gagaccgtga
     4801 ccgggcaggg cggcaacggc ctgccgctcc tcgcgccaat gagcgaagtc gcagggcgcc
     4861 ttgcaattga ggcggcaggt ttggctctgc agcaccaaaa tggcggccgc ggtgtcttgc
     4921 ttggaggtgt tcccggtgtt cttccggcgc gggttacgat acttggtgga ggcgtcgcgg
     4981 gaacccaggc cgcgcggatg gctattgggc ttggcgccga cgttacaatt ttggattgct
     5041 cacttcagcg cctttgccag atcgacggat tgttccaagg gcggccaaaa acaaggtttg
     5101 ctacggcggc agcaattgaa gacactgtgt catcctcgga agccgtaatt ggcgccgtgc
     5161 tggtcccggg tgccaccgcg ccgaagctcg ttaccgaacg catgttgagc acgatgaggc
     5221 agggcgccgt ccttgtcgat atctcgatcg accagggagg ctgtttcgaa agctcacatg
     5281 cgactacaca ttccgcccca atgtttctca aaggcggcat tgttcattac tgcgttgcca
     5341 acatgcctgg cgcggttccc gtcacctcga ctcatgcgct caaccacgcc atgcttccct
     5401 atgggctagc ccttgccgaa ggagggcttc gatcgcttgc ccggaaccct ggcctgcgcg
     5461 ccggg
//
LOCUS       DATMPU010000216         6604 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_4497794,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000216 DATMPU010000000
VERSION     DATMPU010000216.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 6604)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6604)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6604
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4497794"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2795)
                     /locus_tag="VNY06_04965"
     CDS             complement(<1..2795)
                     /locus_tag="VNY06_04965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020176667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003682 - chromatin binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007076 - mitotic chromosome condensation
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation SMC family protein"
                     /protein_id="HWX14196.1"
                     /translation="MKLTKLRITGFKTFVEPSEFLIEPGLTGIVGPNGCGKSNLVEAL
                     RWVMGESSSKSMRASGMDDVIFSGGGERPARNMAEVTLFLDNRDRTAPAAFNDGEAIE
                     VSRRIEREAGSTYRVNGREVRARDVQLLFADASTGARSPAMVQQGQIGEIIAAKPQDR
                     RRILEEAAGISGLHSRRHEAELRLKAAEDNLSRLNDILQHIDTQAESLKRQARQAVRY
                     RGLAGEIRRNEALLALINHTEAARALEASGSKLESDLLDVAEKTRAQAEAARLQALAA
                     QEWPLLRSAEAEANSDLQKLVLARETLDGEERRAKSRIAELEHRVAQTTSDIARESAL
                     IEDASSVLARLAEEAARLANMSASKDEIEALRGELAQAEAALATSEKTLTESQDTKAA
                     LEARRVALEATIKEESQRIARLLSEIEMVRKEHGTLISIATTETDMSALQMALDEAFA
                     TARAREEEVVSIERCHAEAREEEIKLRAPAREAESKAQKLETQAATLSKILNAGTGSF
                     WPPVTEEITVAKGYEAALGAALGDDLDASTNPASPAHWAASDGLGDPALPHGVEPLSK
                     LVSAPAPLARRLNQVGIVLRSEGASLRALLKPGQRLVSKEGDLWRWDGFTQAAEAPTP
                     AARRLAEKNRLGDLAIEAQAARAEADALKRDAERAEAKLVAAANVENAARQAHKDALR
                     LIEAAREKKVSAERRDAETAARLSALGEALLRLDAAREEACGKLKQSEALHGELDPAT
                     ELAARVDAARAEAAEDRAAVAEASAKLQTQLREAETLARRLRGIEEERHSWFARSERA
                     KSQVGALEERCAEAAQELQKLKEAPELFDSTRQTLMLQFEAAEDARRRTADSRAEAET
                     RLANADRAARGALDAMSLAREEKARAEARVEAARERCAEIAHMIATELGCSEAELFGL
                     ANIAPGEELPAVESVEKR"
     gene            complement(3496..4338)
                     /locus_tag="VNY06_04970"
     CDS             complement(3496..4338)
                     /locus_tag="VNY06_04970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012591279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HWX14197.1"
                     /translation="MRLLFIGDIVGRAGRAVFLEYIPGLRAAWQIDGVIVNGENAAGG
                     FGITETICADLLAAGADCVTLGNHAFDQREALIFIAREPRMIRPGNYPSGTPGCGAHI
                     IDTASGARVLVMNLMGRVFMDALDDPFAIAAREISACPLGTGCDAFVIDFHAEASSEK
                     QAFAHFVDGRVSLVAGTHTHVPTADHQILPHGTAYITDVGMSGDYDSVIGMDKEEPVR
                     RFMTKLPAARFEPAAGAATLCGIAVELDIRGLATKIAPIRISGRLSQARPKFWETSQE
                     AAVC"
     gene            complement(4340..4915)
                     /locus_tag="VNY06_04975"
     CDS             complement(4340..4915)
                     /locus_tag="VNY06_04975"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012591280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HWX14198.1"
                     /translation="MHLEKAVLRTQGLARRTEAGSFAAAAFAQHLAAAGLTLAMRLRP
                     DVVSAYFPLPNEPSTLPLLDHLSVAGFKTALPVTGKLGTPLVFRLWRPGEPTAKGKMA
                     IEEPSENAPEVAPDLLFVPLAAFDRGGNRIGYGAGFYDRSLEQLRALKPICAVGVAYA
                     AQEFPKIPNDTHDQNLDYVLTERELIDCRAR"
     gene            complement(5020..5174)
                     /gene="ssrS"
                     /locus_tag="VNY06_04980"
     ncRNA           complement(5020..5174)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="VNY06_04980"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            5329..6276
                     /locus_tag="VNY06_04985"
     CDS             5329..6276
                     /locus_tag="VNY06_04985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011470747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone YbbN"
                     /protein_id="HWX14199.1"
                     /translation="MMGKFGRGGDQKNSYRKVANSGEPKPKAAALVKDATTTSFRADV
                     IAESARQPVLADFWAPWCEPCKRLAPVLEKSVQAAAGKVKLVRLNIDEYPEIPARLGV
                     RSIPAVMAFQKGQPVDGFMGALPEREIKGFIERLVGPLDDVADRLAKAEALAVAGDTG
                     TAADVYRAIIAANPGDPPAVAGLAKLLISAGELESAKRALAAVSPSGERDGAIIAAKA
                     ALDLAEQAATLGSSSELTQKIAANPNDHQARFDFAILLNSHNRRADAANELVEIIKRD
                     RAWNGDGARKQLLQFFDAWGATDPATVAARRKLSALLFS"
     gene            complement(6330..6413)
                     /locus_tag="VNY06_04990"
     tRNA            complement(6330..6413)
                     /locus_tag="VNY06_04990"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6378..6380),aa:Leu,seq:taa)
BASE COUNT         1228 a         2014 c         1964 g         1398 t
ORIGIN      
        1 cgcttttcga ccgactccac ggcgggcagc tcctcgcccg gcgcaatatt ggcaaggccg
       61 aaaagttcgg cttcggagca tcctagctcc gtcgcgatca tatgcgctat ctcagcgcag
      121 cgctctcgag cggcttcgac cctggcttcg gcgcgcgcct tttcttcccg cgcaaggctc
      181 atcgcatcga gcgcgccgcg ggcggcgcga tccgcattgg cgaggcgcgt ttcggcttcg
      241 gcgcggctat cggcggtgcg tctacgtgcg tcttctgccg cttcaaactg aagcatcagg
      301 gtctgccggg tggaatcaaa tagctctggg gcttccttga gcttttgcag ctcttgggcc
      361 gcttctgcgc agcgctcttc caaggcacca acttggctct ttgcgcgttc gctgcgggcg
      421 aaccaagagt ggcgttcctc ctcgatgccg cgaaggcgtc ttgcaagcgt ctcagcctcg
      481 cgcaactggg tttggagttt ggcgctcgct tcggcaaccg cagcacggtc ttctgccgct
      541 tccgcacgcg ctgcatcgac acgcgcggct agctccgtcg ccggatcgag ctcgccatgc
      601 aaagcctcgg attgcttaag cttcccgcat gcttcttcac gcgcggcgtc gaggcgaaga
      661 agcgcttccc cgagcgcgga tagtcgcgcg gccgtctcgg catcgcgccg ttcggccgag
      721 actttctttt cgcgtgccgc ttcgatcaag cgcaaggcgt ctttatgggc ttgtcttgcc
      781 gcgttctcca cgttggccgc ggcaacgagc ttggcctcag ctcgctcagc atctcttttc
      841 agcgcatccg cttcggctcg cgcggcttgc gcctctatcg caagatcgcc aagccggttc
      901 ttttcggcaa gccgtcgcgc cgctggggta ggggcttccg cagcctgggt aaaaccatcc
      961 cagcgccaca aatcgccttc cttcgagacg agcctttggc caggcttcag caatgcccgc
     1021 agcgaagcgc cctccgagcg cagcacgata ccgacctggt tgagtcgccg tgcgagcggt
     1081 gctggcgccg agacgagctt tgataacggt tcgacgccat gaggaagcgc gggatcgccc
     1141 agcccgtcgc ttgcggccca atgcgcgggc gaagcgggat tggtcgaggc atcgagatca
     1201 tcgccaagcg ctgcgccaag cgctgcttca taccccttcg cgaccgtgat ctcctcggtg
     1261 acaggaggcc aaaaacttcc ggttcctgca ttcaatattt tggataaggt tgccgcctgc
     1321 gtttcgagtt tttgagcttt gctctcggcc tccctagccg gcgcccggag ctttatttct
     1381 tcttcgcggg cctcggcatg gcaacgctcg atactgacca cttcttcttc gcgcgccctg
     1441 gctgtcgcga aggcttcgtc gagcgccatc tgcaaagcgc tcatgtccgt ctctgttgta
     1501 gcgatagata tcagcgtgcc gtgttctttc cgcaccattt ctatctcaga gagaagccgc
     1561 gcaatccgct ggctctcctc tttgattgtt gcctccagcg ccacgcgcct tgcttccagc
     1621 gcggccttag tatcttggga ttcggttaat gtcttttcac tggtggcgag agcggcttcg
     1681 gcttgcgcga gttcgccccg caaggcttcg atttcgtctt tggaggcact catattggcg
     1741 agcctggctg cttcctcggc aagccgcgca agcacgctcg atgcatcctc gatcagcgcc
     1801 gattcacggg caatatcgct cgtcgtttgc gcgacacgat gttcgagctc ggcgatacgg
     1861 ctttttgccc gcctttcctc accatccaat gtctcgcggg cgagaacgag tttttgtaga
     1921 tcggaattgg cctcggcttc ggcgctgcgc aacagtggcc attcctgcgc ggccagcgct
     1981 tgcaagcggg cggcctcggc ttgggctctt gtcttttccg ctacgtccag cagatcggat
     2041 tcgagtttcg atcccgacgc ttcaagcgcc cgcgcggcct cggtgtggtt gatcagcgcc
     2101 agcaaggctt cgttgcgccg gatttcgccc gcgagccccc ggtagcgcac cgcctggcgc
     2161 gcttgccgct tcaggctttc ggcttgggtg tcaatgtgct gcaatatatc attgaggcgg
     2221 gaaagattat cctcggcagc cttgaggcgc agctcggcct catggcggcg ggaatggagc
     2281 ccggaaatgc ctgcggcctc ctcaagaatg cggcgccggt cttgcggctt tgcggcgatg
     2341 atctcgccga tttgaccttg ctgcaccatg gcgggggaac gcgccccggt cgaggcatcg
     2401 gcgaagagca attgaacatc gcgggcgcgc acctcgcgac cattgaccct gtaggtcgag
     2461 cctgcctcgc gttcaatgcg gcgggaaacc tcgatcgctt caccatcatt aaatgcggct
     2521 ggtgccgtac gatcgcgatt gtcgaggaag agagtgacct ccgccatgtt gcgcgcgggc
     2581 ctctcgccgc cgccggagaa aatcacatca tccatgcctg aggcgcgcat gcttttcgat
     2641 gagctttcgc ccatgaccca gcgcagggcc tcgacgagat tggatttgcc gcagccattg
     2701 ggtccgacga tgccggtcag cccaggttcg atcaagaatt cggaaggctc gacaaatgtc
     2761 ttgaagcccg tgatgcggag tttggtgagt ttcatcggtt accacccgat gctgcgaacg
     2821 cgcacttgga gcaaggtgac gctttttcga atcatcgtct cgctctcatt cgtcacttag
     2881 ccgtgtgttt ggatgcagaa cggttcgaac ttttgctgga catgctttgc taatattcaa
     2941 cgaagcttct tagcttttgg ttaaccaatc acggcggcca gaaggccttg atccgattga
     3001 ggtgattcga tttggctcga ttacggccca acggccgtgg cgaacgcaag aattcggaag
     3061 gatttttctg tgaaagccgc aaatctcttg tttccgcttg cggcgcgcgc cggcggggcg
     3121 gtacgcaggg ctcgatggtc tttgggcggc agcgttcaac cgcctcgtct tctacgtgcg
     3181 ctaaagcagc gccatcgtga ctggcaccca aattttgatg ctcggcaaat tttagcggcg
     3241 gcagagtttt tcagcgattt tgtggttgcg caattctcgc cagattcgac ttcggctgct
     3301 tccgtcttaa tgaaagtgat gcacgccaaa acgtctgcgg caccctgcga aaccagaagc
     3361 tctgagcctt acgggtccat aaccagcgca tcatagccgg ggatggcggt ccgccatacc
     3421 ataatcgcgc cgatggcgtc gaggatgatc ccgcgatttt gtcctcgcca gtctccgaat
     3481 agacatcctt caatgctaac aaactgccgc ctcctgggac gtttcccaaa atttcgggcg
     3541 ggcttgcgac agcctgccgc tgatacgaat aggcgcgatc tttgtcgcaa gaccccttat
     3601 gtcaagctcg accgcgatgc cgcaaagtgt cgccgccccg gcagcgggct caaaacgcgc
     3661 ggcgggcagt ttggtcatga agcggcgaac cggctcctcc ttgtccatcc cgatgacgga
     3721 atcataatcc ccgctcatcc cgacgtcggt tatgtaggcc gttccgtgcg gcaagatctg
     3781 atggtcggcg gtcggcacat gtgtatgggt ccctgcgacg agcgaaaccc ggccatcgac
     3841 aaagtgcgcg aaggcttgct tttcgctcga tgcctcggcg tggaaatcga tgacaaaggc
     3901 atcgcaaccc gtgcccaatg ggcaggccga tatttcccgc gcggctatcg cgaagggatc
     3961 gtccaaggca tccatgaaga cgcgtcccat gaggttcata acaaggacgc gggcgccaga
     4021 agccgtgtca ataatatggg cgccgcagcc gggtgtgccg ctcgggtagt tcccgggccg
     4081 gatcatccgt ggttcgcggg cgatgaagat aagcgcctcg cgttggtcga aggcatgatt
     4141 gccgagtgtc acgcaatctg cccccgccgc gagcaaatcc gcgcagatcg tctccgtgat
     4201 cccaaagcct ccagccgcat tctccccatt gacaatgaca ccgtcgattt gccatgcggc
     4261 gcgcagcccg ggtatgtatt ccaagaacac ggcacgaccg gcccggccca cgatgtcgcc
     4321 gatgaagaga agacgcattc tatcgtgccc ggcaatcgat caattctcgt tccgttagca
     4381 cgtaatcgag gttttggtcg tgcgtatcgt ttggaatctt gggaaattcc tgggcagcgt
     4441 aggctacgcc cacggcgcaa atcggcttaa gtgcgcgcaa ttgctccagg ctgcgatcat
     4501 aaaaaccggc gccgtagcca attctgttgc cgccccggtc gaaagccgcc aatggcacga
     4561 acagcaaatc gggcgcaacc tcaggtgcgt tctctgacgg ttcttcgatt gccatttttc
     4621 ctttagcggt cggctcaccc ggacgccaaa gccggaacac gaggggcgtg ccaagcttac
     4681 ctgtcacggg aagtgctgtt ttgaaacctg ccacagagag gtggtcaagc agcggcaggg
     4741 tcgaaggctc atttggcaga gggaaatagg ccgagacgac gtccgggcgc aaccgcatcg
     4801 ctaaagttag gccggcagcc gcgagatgct gggcaaatgc ggcggctgca aagcttcccg
     4861 cctccgttcg ccgcgccagc ccttgcgtgc gcaagactgc tttctcgaga tgcatgctaa
     4921 atggcccaaa ctgccccgga attcacccaa atgggtaaaa tggggatcga aaagctatcg
     4981 cgtggcttcg atcggcaagg gcagagaaga cccaaactat acggggccgc gatggccgtg
     5041 ggacaccggt cccgggtacc tacaaagtag gtgggcgccg tgtggccaag cccacgagcc
     5101 tggccaggga cagctccctt caagaccgat aaggccccgg ggaatctggt ccaacacgca
     5161 ccccgcagct ccgccgcatg aatttagtgt aagtttgctc caaatgccag ggctgcagag
     5221 cggcggggcc agatggttgc gatttcggct ctgatgccgc atttacatct caattgtttg
     5281 gggattttcg ccggtatgcc gccggcatag caatagctgg aggttcagat gatggggaaa
     5341 tttggccgcg gcggcgacca gaaaaacagc taccgcaagg tcgcgaattc cggggagccg
     5401 aaacccaagg ccgcagcgct tgtgaaggac gctacgacga cgagttttcg cgccgatgtg
     5461 atcgcggagt cagcccgcca gcctgtgctg gccgattttt gggcgccctg gtgcgagccc
     5521 tgcaagcggt tagcgcccgt gctcgaaaaa agcgtgcaag ccgcagccgg taaggtcaag
     5581 ctcgtcaggc tgaatattga cgaatatccg gaaattcctg ccaggctcgg ggttcgttcc
     5641 atcccagcag tcatggcctt tcagaaaggc cagccggtcg atggctttat gggtgccttg
     5701 ccagaaagag agatcaaggg ttttattgag cggcttgtcg gcccattgga tgatgtagct
     5761 gatcgcttgg ccaaggccga agctttggcc gttgccgggg acaccgggac cgcggcggat
     5821 gtctatcggg cgatcatcgc cgcaaaccca ggggacccgc ccgctgtggc cggcttggca
     5881 aagctcctta tatcggcagg cgagctcgaa agcgcaaagc gcgcgctcgc cgcagtgagc
     5941 ccgagcggtg agcgcgatgg cgcaatcatt gccgccaaag ccgcgctcga tcttgctgaa
     6001 caggccgcaa cccttggcag ttcgtccgag cttacgcaaa agatcgcggc caacccaaat
     6061 gaccatcagg cacggttcga tttcgccatc cttctcaatt ctcacaaccg gcgtgcagac
     6121 gcggcaaacg agctcgttga aatcatcaag cgcgatcggg cgtggaacgg cgatggcgcg
     6181 cgcaaacagc tcttgcaatt tttcgacgct tggggcgcaa ccgatccggc aacagtcgcc
     6241 gcaaggcgca agctctccgc cctcttattt tcttgaagaa tgcaaatgcg ggcgccagct
     6301 gcatgccttg ctctcccgat gcggagctct ggtgcggacg acgggagtcg aacccgtatg
     6361 accgaagtcg agggatttta agtcccttgc gtctaccgtt tcgccacgtc cgcttggccc
     6421 aaacgggctt aaggcgcaag caatgccaga caagcgcgac cttacatcgg aaaggcccgc
     6481 ggacaactcg ccgcgcccga tagcgttgcc ggcgcccgac tcgaatcgta ccggaaaatc
     6541 gatcaatttt acaaattgaa ggtgtggccg ctgtcttcga ccttgtaagg tcttgggccc
     6601 cgac
//
LOCUS       DATMPU010000217         4187 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_1416793,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000217 DATMPU010000000
VERSION     DATMPU010000217.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 4187)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4187)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4187
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1416793"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(108..2615)
                     /gene="clpA"
                     /locus_tag="VNY06_04995"
     CDS             complement(108..2615)
                     /gene="clpA"
                     /locus_tag="VNY06_04995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012589788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0043335 - protein unfolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HWX14200.1"
                     /translation="MPSFSRNLEQSLHRALAIANERHHEYATLEHLLFSLTEDGDAAA
                     VMRACSVDLDILRKNLLEYIDQELDNLVSEDREDAKPTAGFQRVIQRAVIHVQSSGRE
                     EVTGANVLVAIFAERESHAAYFLQEQDMTRYDAVNYISHGIAKRPGLSDSRRVPRGAE
                     EEAEAREAREAKESGESKKKEGALDAYCVNLNKKARDGRIDPLIGRENEVQRTIQVLC
                     RRHKNNPLLVGDPGVGKTAIAEGLARKIVMGDVPEVLADATVFALDMGTLLAGTRYRG
                     DFEERLKQVMKEIEHHKKAILFIDEIHTVIGAGATSGGAMDASNLLKPALAQGVLRCI
                     GSTTYKEYRQYFEKDRALVRRFQKIDVNEPTIADAIEIMKGLKPYFEEFHKLRYTNEA
                     IKAAVELSARYINDRKLPDKAIDVIDETGASQMLLPEAKRKKTIGVKEVETTIATMAR
                     IPPKTVSKDDAEVLEHLTETLERVVYGQNEAIEALTSAIKLARAGLRDAEKPIGCYLF
                     SGPTGVGKTEVGRQLAISLGVELVRFDMSEYMERHTVSRLIGAPPGYVGFDQGGLLTD
                     AIDQHPHCVLLLDEIEKAHPDLYNILLQVMDHGKLTDHSGKSVDFRNVILIMTTNAGA
                     ADMARAAYGFTRSKREGDDMEAISRMFAPEFRNRLDAVVTFRHLPAEVIVKVVDKFIM
                     QLEAQLADRNVAIELTDEARKWLVEHGYDEQMGARPMARVIQQAIKTPLADEVLFGRL
                     KNGGTVRVVVTANEDGAKKLGFVYPEGPVLPRPERDIVEASKKRVRPEPEVRRARSLR
                     HKGEDGGDDDEPGEDLAGPDVDEAETIGPSGEPDTKH"
     gene            complement(2629..2973)
                     /gene="clpS"
                     /locus_tag="VNY06_05000"
     CDS             complement(2629..2973)
                     /gene="clpS"
                     /locus_tag="VNY06_05000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016918997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HWX14201.1"
                     /translation="MAAAKEPRREGGEDGPGSGTAVITRPKPLTRHPNMYRVLLLNDD
                     YTPMDFVVAVLRKYFNKNADEATRIMLHVHQHGVGECGVFTYEIAEMKVTQVMDHARK
                     HQHPLQCIMEKK"
     gene            complement(3440..3700)
                     /locus_tag="VNY06_05005"
     CDS             complement(3440..3700)
                     /locus_tag="VNY06_05005"
                     /inference="COORDINATES: protein motif:HMM:NF020918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phasin family protein"
                     /protein_id="HWX14202.1"
                     /translation="MSLADGFRQFASESACYTQELVDRGYAFAGELRQAKSPVAAVQV
                     QIDFARSNYVRLLDHFLRVSTLSWDLLRQACKPVEGQAAKVK"
     gene            complement(3724..3963)
                     /locus_tag="VNY06_05010"
     CDS             complement(3724..3963)
                     /locus_tag="VNY06_05010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX14203.1"
                     /translation="MAKQTKATAAETGNRKEGGESEKKTYPASRSAIIEPEADIDADQ
                     MPSGDSAAGGGNHPMVATVEAVVTPEFEILEKLGK"
BASE COUNT          828 a         1238 c         1159 g          962 t
ORIGIN      
        1 gcccgaaagg tggtgcggta cacgtcgaca tgggcgtcga cgcttagcgt gccggtgcgg
       61 cgaggggcgg agcggaagag gaagctccgg ccatcaatgt aatggcgtca gtgctttgta
      121 tcaggctcgc cacttgggcc aatcgtctcg gcctcatcga catcggggcc ggccagatct
      181 tcacccggct cgtcgtcatc accgccatct tcgcccttat ggcgaagcga cctcgcacgc
      241 cgcacttcgg gttccggacg gacccgtttc ttgctcgcct cgacaatgtc gcgctccggc
      301 cgcggcagca ccggcccttc gggatagacg aagcccagtt tcttggcccc atcttcattc
      361 gcggtgacga caacccgcac cgttccgccg tttttcaacc gcccgaaaag cacttcatcg
      421 gcgaggggcg tcttaatcgc ctgttgaatg acgcgcgcca tgggacgcgc gcccatctgc
      481 tcgtcatagc catgttcaac gagccacttg cgcgcctcgt cggtgagttc gatcgcgacg
      541 ttccggtcag cgagctgagc ctccaactgc ataatgaatt tgtcgaccac tttgacgatg
      601 acttctgcgg gcagatgccg gaaggttaca accgcatcga gccggttgcg gaattccggc
      661 gcgaacatcc ggctgatcgc ctccatatcg tcgccctcgc gtttcgagcg ggtgaatcca
      721 taggcggcgc gggccatatc ggcagcaccg gcatttgttg tcatgatcag gatcacgtta
      781 cggaagtcta cggatttacc gctgtgatcg gtgagctttc cgtgatccat aacctgcaac
      841 agaatattgt agagatcggg atgggctttt tcgatctcgt ccaagagcag cacgcaatgg
      901 ggatgctggt cgatcgcgtc ggtcaaaagg ccgccttggt cgaagccgac atagccgggg
      961 ggagcgccga tgagccggga gacggtgtgg cgctccatat attccgacat gtcgaagcgc
     1021 acgagctcga caccaagact gatagcgagc tgacgcccaa cttcggtttt gccaacgccg
     1081 gtcggcccag agaagagata gcatccgatc ggcttctccg cgtccctaag gccggcacgg
     1141 gcgagcttaa tcgccgatgt caatgcttcg attgcttcat tttgaccata gacgacgcgt
     1201 tcgagcgtct cggtcaaatg ttcgagaacc tccgcatcgt ctttggaaac ggttttggga
     1261 ggaatccggg ccatcgtcgc gatggttgtc tcgacttcct taacaccgat cgtctttttc
     1321 cgcttggctt cgggcaagag catctggctc gccccggttt catcgattac atcgatcgct
     1381 ttgtccggca atttccggtc attaatgtag cgagcggaaa gttcgaccgc ggcctttata
     1441 gcttcgttgg tgtaacgaag cttatggaat tcctcgaaat agggttttag gcccttcatg
     1501 atttcgatgg cgtcggcaat tgtcggttca ttgacgtcaa ttttttggaa gcggcgcaca
     1561 agcgcgcggt ccttctcaaa atactgccgg tactctttgt aggttgtcga accgatacag
     1621 cgcagaacgc cttgtgccaa agcaggttta agtaagttcg acgcatccat cgcaccgccc
     1681 gacgtcgcgc cagccccgat caccgtgtgg atctcgtcga tgaagagaat tgccttctta
     1741 tggtgctcga tttccttcat gacctgtttg aggcgttctt caaaatctcc gcgatagcgc
     1801 gttcccgcga ggagcgtgcc catgtcgagc gcaaacaccg tcgcatcggc gaggacctcc
     1861 ggtacatcgc ccatgacgat tttgcgggcg agcccttcgg ctatagcggt cttgccaacg
     1921 ccagggtcac cgacgagcag cgggttgttc ttgtgacggc ggcacaagac ctgaatggtg
     1981 cgctggacct cgttttcgcg gccaatcagc ggatcaatgc gcccatctcg cgccttcttg
     2041 ttgaggttga cgcagtaggc atcaagagcg ccctcctttt tcttcgattc gccgctttcc
     2101 ttagcctccc tcgcttcacg cgcttcggct tcctcctcag cgccacgcgg aactctcctc
     2161 gagtcggaga gacctggccg cttggcgatg ccgtggctga tatagttgac cgcgtcatat
     2221 cgggtcatgt cctgctcttg caggaaataa gcagcgtggc tttcgcgctc ggcaaagatg
     2281 gcaacaagca cgttggcgcc ggtgacctct tcccttcccg aggactgaac gtgaatgacc
     2341 gcccgctgga tgacgcgctg gaaccccgcc gttggtttgg cgtcttcgcg atcctcgctc
     2401 accaaattgt cgagctcttg atcgatgtat tcgagaagat tttttcttaa aatgtcgagg
     2461 tcgaccgaac aggcccgcat cactgccgcg gcgtccccat cttcggtcaa actgaacaac
     2521 aaatgttcaa gcgtggcata ttcatgatgg cgctcattgg caattgccaa ggccctatga
     2581 agcgactgtt cgagattgcg ggagaaggac ggcattggct ccaacctctc atttcttttc
     2641 cattatacac tgcaacgggt gctgatgctt gcgggcgtga tccatgacct gcgttacttt
     2701 catctcggcg atttcgtaag taaagacgcc gcattcgcca acgccgtgtt gatggacatg
     2761 caacataata cgcgtcgctt cgtcggcatt tttgttgaaa tacttccgta atacagccac
     2821 cacaaagtcc attggcgtgt agtcgtcgtt caacagcaag acgcgataca tgttggggtg
     2881 ccgcgtcagc ggctttgggc gggttataac agccgtgccc gaccccgggc cgtcctcgcc
     2941 accctcccgc cgcggttcct tagccgctgc aatctcacag gcgggcaagg ccgcgcgcca
     3001 aaccgcatcc gtgggagttc ccctgtaagc caaaagctga gatagccgag tggctccgtc
     3061 catcacaagc aaacctcgcc gactgaaaac ctcgcttgcc gccagtcgcc tgccggcctt
     3121 ggcatccatc attcaacctc gcgccaggcc atgctggcaa ctggcaaaac ataatttagc
     3181 gctggttcgc ttcgggagcc atacgacatt ttgttcaaat caaatgaatt tcgcttgacg
     3241 cctgccgcca ggcttttgga ccgaaaacac ttcacgcagc gcccaaatgt cataggaggg
     3301 atagccaatt gaaaaagccc ggctccgccg ggcttgaaaa taaagcagga aggcttcggg
     3361 ccgcccaagc atttcgatca gggcaatgtc tggtgcttgg ccgcgacgga agggaggcct
     3421 gcgggatgca ggcggcgcct tacttgacct ttgccgcctg cccttcgaca ggtttacaag
     3481 cttggcggag aaggtcccaa gagagcgtgc tcactctcaa aaagtggtca agaaggcgga
     3541 catagtttga tcgggcaaaa tcaatttgaa cctgaaccgc ggctaccggg gactttgctt
     3601 gccgcaactc tccggcaaaa gcatagccgc gatccacaag ttcttgggtg tagcaagccg
     3661 attcgctcgc aaactggcga aatccatcgg caagcgacat gccatttcgg tgacttccaa
     3721 cccctacttg ccaagtttct ccagaatttc gaattctgga gttacgaccg cttcgacggt
     3781 tgccaccatc ggatgatttc cgcccccagc ggcactgtcg ccgctaggca tttggtcagc
     3841 gtcaatatca gcttctggct cgataatggc agatcgagag gctggataag ttttcttctc
     3901 agactctccc ccctccttgc gattaccagt ctcggcagcc gtagccttgg tctgtttggc
     3961 catgtgagtc tcctttgcca cttatgctgg cgctcgggtt tatgccggcg ctcgcgccgg
     4021 taccactggc caactcgctt tgtcggcggc caattgcgga ccgagcgtgc aacgctacga
     4081 gcgggatagg ccccaagcgc agcaggctag agcgtgtttt cggctgtgca attcgaccgg
     4141 cgcccagtac ggccttaact ctttttgggc ggacactggt agagctt
//
LOCUS       DATMPU010000218         7326 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_3888649,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000218 DATMPU010000000
VERSION     DATMPU010000218.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 7326)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7326)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7326
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3888649"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..353
                     /locus_tag="VNY06_05015"
     CDS             <1..353
                     /locus_tag="VNY06_05015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014889759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX14204.1"
                     /translation="RGTTRVRVEYVGAASLAGSDDQRLLATLRLDGPATLLEPAVTGR
                     GRTDVAENMPTVVARAETQATNSQMDSASSQMTAGPAVYFKGRPVPLPPARPLTLGPN
                     PGGSTRLALRRASF"
     gene            607..1830
                     /locus_tag="VNY06_05020"
     CDS             607..1830
                     /locus_tag="VNY06_05020"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012384527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HWX14205.1"
                     /translation="MPTNLWNVRLRELRISSLTEAVFPVLFFAWLAGALSPAFAQTFQ
                     TSAPHAILIDAATRSVLFEKDADALVVPASTVKIMTAEIVFHELALGHLHLDDQFVVS
                     ESAWRLGGAPARGSAMFAAPNSSIRIEDLIRGLVIVSGNDAAITLAEGIAGSEGAFAT
                     QMTRRARELGLEHSTFTNAWGRGDPDQKVSPREMVTLANHIIQTYPDYYKYFGERDFT
                     WNKIKQPSRNPLLTMDLGADGLKTGNIDESGYGLIGSAVQNGQRLILALYGSRTAKER
                     AEEARKILLWGFRSFDSRTIFQAGEIVGGAKVYGGNSSEVPLVADGEVRILVPRSQAE
                     KFTGRIVYAGPLVAPVEAGREVARLKIFHGTEQVLDLPLKTAAAVEQGSLPRRAMDAG
                     IEYVTVFFRKYVLKK"
     gene            1827..2528
                     /gene="tmk"
                     /locus_tag="VNY06_05025"
     CDS             1827..2528
                     /gene="tmk"
                     /locus_tag="VNY06_05025"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018408313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HWX14206.1"
                     /translation="MTTARAQLPSPSARGRLITLEGGEGTGKSTQLAHVVRWLQQSGI
                     EVIGTREPGGSPGAEILRRVLLSGRVASLGPSAEAILFAAARIDHIDATIEPALSEGK
                     FVVCDRYADSTRAYQGARGQLDPRFLRALERVTSGIVRPDLTLIFDLPAAKGLARADF
                     RRGRGEGPDRFEGEDLPFHENLRAAFLAIAAAEPERCAVIDASRSEEDVAQAIFGVIS
                     NRLLAPAVAQQVRSG"
     gene            2521..3624
                     /locus_tag="VNY06_05030"
     CDS             2521..3624
                     /locus_tag="VNY06_05030"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020176075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HWX14207.1"
                     /translation="MAKSMAQGFSEAGPESDRFEDTPHPRETYDFFGHAKAEGELLRS
                     YLRGCLPHAFIISGLPGIGKATLAWRLARFILANPDPAGVTRADRTDLFVPEGHAISR
                     QVAALSHPDLVLLRREWNPETKRHATQIQVDDVRRAIHMFQRAAGRGGFRICLLDCAD
                     DLNPSSANALLKLVEEPPPRSLFLIVAHQPGLLLATLRSRCRKVHLKPLGSTDIGNIL
                     TALGPPWSNTSEPKRETAIARAQGSVHRTLRLLDEEGIALETAVMRVLDELPQIDWNK
                     AHILADRIAARHNSTDYESALALIDEWLDARVHLGAQDYREDYARRLAPFADVWEKLR
                     EATREMETFNLDKRPFVLSLIADLAAATRAPSS"
     gene            3686..5266
                     /gene="metG"
                     /locus_tag="VNY06_05035"
     CDS             3686..5266
                     /gene="metG"
                     /locus_tag="VNY06_05035"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020176076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HWX14208.1"
                     /translation="MNARPPFYVTTAIPYANGAPHIGHAYERIATDAVARFKRLDGYD
                     VLFVTGMDEHGQKMQQTAAREGLTPQELADRTAAQFAAMGAALFSEADDVVRTTQPRH
                     HLAARELWTRMAATGDIYLSKYPGWYSVRDEAFFDESELVGGPDGTRLGPTGASVEWV
                     EEESYFFRLSAYQDRLLKLYRDQRDFITPAKYRNEIVSFVERGLADLSISRSTFDWGI
                     PVPGDTRHVMYVWIDALANYITGTGFPDSKAARAKFWPASAHVIGKDITRFHAIYWPA
                     FLMSAELALPRQVVVHGFLFNRGEKMSKSVGNVIDPMVLAHHYGADQLRYFLLREVPF
                     GQDGSYSHEAIVNRINADLANDLGNLAQRCLAMVAKNCDGILPTPDALSENDRAIIAQ
                     AIALPAKSRAAMEDFALHAMLTEIWRVVGDTNRYFASEEPWAIRKSQPLRMQTVLYVT
                     AEVLRIVAIMTQPFMPVSAGKLLNLLGVPGNARNLANGEISPGLEPGTKLPRPVPVFP
                     RYINTEEDLSDKADCADR"
     gene            5253..6053
                     /locus_tag="VNY06_05040"
     CDS             5253..6053
                     /locus_tag="VNY06_05040"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018427888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HWX14209.1"
                     /translation="MLIDSHCHLDFEDFATERDAIVERARAAGLARIITISTRTESFT
                     SLSRLADKYSDVFCTVGTHPHNAHEEASVSAEQLTALALHPKCIGIGEAGLDYHYDRT
                     PRDIAAKVFRTHIAAARNSGLPIVVHARDADADIAAILRSEMENGAFTGLLHCFTSSP
                     MLADVALSLGFCVSFSGVLTFKNSQGLREIASRVPMDRLLVETDAPFLAPVPFRGKRN
                     EPAFVVATARKLAEVKGVSLEIIAAETSANALRLFTKMPAPTTGAEAG"
     gene            6050..6847
                     /locus_tag="VNY06_05045"
     CDS             6050..6847
                     /locus_tag="VNY06_05045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020176078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HWX14210.1"
                     /translation="MSVTFTILGCGSSGGVPRLGQGWGACDPNNPKNRRRRCSLMVER
                     AGDNGAKTHVLVDMSPDLREQLLGLEISRLDAILLTHSHADHIHGIDDVRPLVIKARR
                     KIDLFMDAATSAVVRTNFGYVFETPAGSQYPALLTEYRLVSGYSITINGPGGPLTTTP
                     FQLEHGEIGALGFRFGQIAYTPDVNAIPPESVAFLEDLDVWIVDALRYTPHPSHFCLD
                     ETLHWIKKLRPARAILTNLHSDLDFDRLRSELPANVEPAFDGMRISA"
     gene            6997..>7326
                     /locus_tag="VNY06_05050"
     CDS             6997..>7326
                     /locus_tag="VNY06_05050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012384518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX14211.1"
                     /translation="MGFPIGVAKAADNCYAFLVTGFSAFGIWNSDNRRAFGLVPLGCR
                     RAFACAFSGWAEELVFGSGARRHAIFFEDFLADTNRHTMSKGKDFRGPKKRGFDDDGP
                     SPYDSPRM"
BASE COUNT         1563 a         2054 c         2071 g         1638 t
ORIGIN      
        1 aaagaggaac tacccgagtt agggtcgaat atgtcggtgc cgcgagcctt gctgggtcgg
       61 atgatcaaag gcttcttgcg acattgcgtc tagacggccc agccacgctc ttagagccag
      121 cagtcactgg gcgaggtcga accgatgttg ccgaaaacat gcctacagtg gtcgctcgtg
      181 ctgaaacaca ggcaacgaat tcgcaaatgg acagcgcatc gagccaaatg actgcaggtc
      241 ccgccgtcta ttttaagggc aggccggtac ctctgccccc agcgcggcca ttgacgttgg
      301 gaccaaaccc cggtggcagt acaaggctag ccttgcgtcg ggcgagcttt tagggaactg
      361 cgaggcttcc cgatccagcc tcgaatgccg aggcgtgatt ttttatgaaa ttttcggccg
      421 aatgcgtcgc cagcgcccca caggtgttga tcatcgccca aacccgcggt acgacaattt
      481 caagcgagct agctcagctc gattccaaca gtcacatttg gctagccgtc cttgccatcg
      541 ctttttaggg ctcggcgcac ggacgcgggt aacggaacta tagattttgc cctgctgcgg
      601 tacgtcatgc ctacaaattt atggaacgtt aggcttcgtg aactccggat ctcgagcctg
      661 accgaagcgg tctttccggt gctgtttttc gcgtggctcg ccggggcgct ctccccagcc
      721 ttcgcgcaga cgtttcaaac cagcgcgccg catgcgattt tgatcgatgc agccacaaga
      781 agcgtgctgt tcgaaaagga cgccgatgcg ctcgtcgtcc cggcatcgac ggtgaaaatc
      841 atgaccgccg aaatcgtgtt tcacgaactc gctttgggcc atctgcatct cgacgatcag
      901 ttcgtcgtca gcgagtcggc ttggcggctc gggggtgcgc cggcgcgcgg atccgccatg
      961 ttcgccgccc ctaacagcag cataagaatc gaggatttaa ttcgcggctt ggtaattgta
     1021 tccggaaatg acgccgctat cacgcttgcc gaaggaattg cgggttcaga gggagccttc
     1081 gccacgcaga tgacgagacg cgcgcgcgag cttggcttag agcactcgac atttaccaat
     1141 gcttggggca ggggcgatcc cgatcaaaaa gtaagcccaa gggaaatggt gacgctcgcc
     1201 aatcacatca tccagaccta tccggattat tacaaatatt ttggtgaaag ggacttcacc
     1261 tggaacaaga taaaacagcc gagccgcaat cccttattga ccatggatct cggcgcggac
     1321 gggctcaaga ccggcaatat cgacgaaagc gggtacgggc tcatcggctc cgctgttcaa
     1381 aatggccagc ggctgatcct ggcgctgtac ggcagccgga cagccaaaga acgtgccgaa
     1441 gaagcccgca aaatcctgct gtgggggttt cgctcattcg attcgagaac gatttttcaa
     1501 gccggggaaa tcgtcggcgg cgccaaagtt tacggtggta acagctcgga agtgccactt
     1561 gtggctgacg gggaagtcag gattctcgtg cccaggagcc aggccgaaaa atttacgggt
     1621 cgcatcgtct acgcaggtcc gctcgtcgca cctgtggagg ccgggagaga ggtcgcgcgc
     1681 ctcaagattt tccacggaac agagcaggtt ctcgatctac ctttgaagac cgcggcggcg
     1741 gtagagcaag gatcactgcc gcgtcgggcc atggatgccg gtattgaata tgtgactgtg
     1801 tttttccgca aatatgttct caagaaatga ccacggcacg cgcgcaactc ccttcgccta
     1861 gtgcgcgtgg tcgtctgatt acgctcgaag gcggtgaagg aacaggcaaa tcgacccagc
     1921 ttgctcatgt tgtgcgttgg ttgcagcaaa gcggcatcga ggtgattggc acccgtgagc
     1981 caggcggatc tccaggcgcc gaaattttgc gtagggtcct gctttcaggc cgcgtggcga
     2041 gccttggtcc gtctgcggaa gctattcttt tcgctgcggc caggatcgac cacatcgacg
     2101 caacgatcga acccgcactc tccgaaggaa agtttgtcgt ctgtgataga tatgctgact
     2161 caaccagagc ttatcagggt gcaaggggac aactcgaccc tcgctttttg cgcgcattgg
     2221 aacgtgtaac ttcgggcata gtgcggcctg acctaacctt gatcttcgat cttcccgcgg
     2281 cgaaaggctt agcccgcgca gattttcggc gtggccgcgg tgaaggcccc gaccgatttg
     2341 agggagagga tttgccgttt catgaaaacc ttcgtgccgc gtttcttgcg attgcggcgg
     2401 cggaacctga gcgctgtgcc gttatcgacg catcccggtc cgaagaggat gtagcgcaag
     2461 cgattttcgg cgtcatttca aatagactcc ttgctcccgc tgtagcccag caggtgcgca
     2521 gtggctaagt cgatggcgca aggattctca gaagcagggc cagagtccga ccgtttcgag
     2581 gacaccccgc atccgcgcga gacttacgat tttttcggcc atgccaaggc ggaaggggaa
     2641 cttcttcggt cttatttgcg gggctgtttg ccgcatgcat tcatcattag cggactgccc
     2701 ggaattggca aagccacgct cgcttggcgc ctggcgcgct ttatattggc gaaccctgac
     2761 ccagctggag tcacgagagc agacagaacg gacctgtttg tgccggaagg ccatgcaatt
     2821 tcccgtcaag tcgcagcgct ctcgcaccct gatcttgtgc ttttaagacg cgagtggaac
     2881 ccggaaacaa aaaggcacgc gacgcaaatc caagtggacg acgtccgccg cgccattcac
     2941 atgttccagc gggcggccgg gcggggcggt ttccggatat gcctcctcga ttgcgccgat
     3001 gatctcaatc cttcgagtgc aaatgcgctc ctgaaactcg tcgaggaacc gccgccccgc
     3061 tcgctatttc tgatcgtcgc gcaccagcca ggccttttac ttgcgacctt gcgttcacgc
     3121 tgccggaagg ttcatcttaa gccgctcggc tccacggata ttggcaatat tctgaccgcg
     3181 cttggaccgc cgtggtccaa caccagcgaa ccgaagcgcg agaccgcgat cgcgcgcgcg
     3241 caaggctcgg ttcatcgcac cttgcggctt ctcgacgaag aaggaatagc gctcgaaacg
     3301 gctgtcatgc gcgtcttgga cgaactgccg cagatcgatt ggaacaaggc gcatatctta
     3361 gccgaccgga tcgccgcgcg ccacaacagc acggattacg aaagcgcact tgccttaatc
     3421 gacgaatggc ttgacgctag agtgcacctt ggcgcgcaag actatcgaga ggattatgcc
     3481 aggcggcttg cgccctttgc cgacgtgtgg gaaaaactac gcgaggcaac gcgcgagatg
     3541 gaaacattca acctcgataa gcggcccttt gtcctgtccc ttatcgccga tttggcggct
     3601 gcaacgcggg caccctcatc ctaaggcatg attcccaagg gccatcgtct ggcaactctc
     3661 cttgacacat gatgatcgga aattcatgaa tgcccgcccg ccattctatg tcacgaccgc
     3721 aattccttac gccaatgggg cgccgcacat cggacatgcc tatgagcgta ttgcgaccga
     3781 cgccgtcgcg aggttcaagc gactcgacgg ttacgatgtg ttgtttgtca caggcatgga
     3841 tgaacatggc cagaagatgc agcagacagc tgcacgcgaa ggacttacgc cccaagagct
     3901 tgccgaccgc actgcggcgc aatttgccgc gatgggagct gctctttttt ccgaggccga
     3961 cgatgtcgtc cggacaactc agccacgcca tcatctggcc gcgcgcgagc tttggacccg
     4021 aatggcagcc acgggtgata tctatctttc caaatatccg ggctggtatt cggttcgcga
     4081 tgaagccttt ttcgatgagt ctgagctggt cggtgggccg gatggcacac gtctcggccc
     4141 aaccggtgcg tcggttgaat gggtcgagga agagagctac ttttttaggc tttcagccta
     4201 ccaggaccgg cttctcaaac tgtaccggga ccagagggat ttcataacgc cagctaagta
     4261 tcgaaacgaa atcgtctctt ttgtcgagcg aggcctagct gatctttcca tcagccgttc
     4321 gaccttcgac tggggtatcc ccgtgcctgg cgacacgcgc catgtgatgt atgtctggat
     4381 cgacgcgctg gcgaactata tcaccggaac aggtttcccg gattccaaag cggcgcgcgc
     4441 aaaattctgg ccggccagcg cccatgtgat cggcaaggac attacaaggt ttcacgcgat
     4501 ttattggccg gccttcctta tgtcggccga gttggcactg ccacgccagg ttgtggttca
     4561 cggctttttg ttcaatcggg gcgagaaaat gtcaaagtcg gtcggcaatg tgatcgatcc
     4621 gatggtgctc gcccaccatt acggcgccga ccaattgcgt tattttttgt tgcgcgaagt
     4681 accgtttggc caagacggca gctattccca tgaagccata gtcaaccgaa tcaatgcaga
     4741 tcttgctaat gatctcggca accttgcgca gcgttgctta gctatggtgg ccaagaattg
     4801 cgacgggata ctgcccacgc cagatgcgct tagcgaaaac gaccgcgcaa taattgccca
     4861 ggcgatcgca cttcccgcga aatcccgtgc cgcgatggaa gactttgcct tacatgcgat
     4921 gctaaccgag atttggcgcg tggtcgggga tacgaaccgc tattttgcaa gcgaagaacc
     4981 atgggccatc cgcaagagtc agccgttgcg gatgcaaacg gtcctctatg ttaccgccga
     5041 ggtcttgcgt atagtcgcga tcatgacgca gccattcatg ccggtgtcgg caggaaagct
     5101 tcttaacctt ctgggtgttc ccggaaacgc gcggaatctt gccaatggcg aaataagtcc
     5161 gggcctcgag cccggcacaa aattgccccg gccggtgccc gtttttccac gctacatcaa
     5221 tactgaggaa gatttgtccg ataaggccga ttgtgctgat cgatagtcac tgccatctcg
     5281 atttcgaaga ttttgccact gagcgcgatg caatagtgga acgcgcgcgt gccgccggcc
     5341 ttgcgcgaat catcactatt tcaacccgca cagagtcctt tacgtcgctg agccgactcg
     5401 ctgacaaata tagtgacgtc ttttgcactg tcgggactca tcctcacaac gcgcatgagg
     5461 aagccagtgt gtccgcggaa cagctaaccg ctttggcgct ccatccaaaa tgcatcggca
     5521 ttggcgaagc gggcctcgat tatcattacg acagaactcc gcgtgacatt gcagctaagg
     5581 tattccgtac tcatatcgca gccgctcgaa atagcgggct gccgattgtg gttcatgccc
     5641 gtgatgcgga cgccgacatt gccgcaatcc ttcgcagtga aatggagaac ggtgcattca
     5701 caggtcttct ccattgcttt acgagcagtc ctatgctggc ggatgtcgca ttgtcactcg
     5761 ggttctgcgt ttcgttttcc ggcgttttga cgttcaagaa ctcgcaaggg ttacgggaaa
     5821 tcgcgagcag ggtgccaatg gaccggcttt tggtcgaaac cgatgcgccc tttctggcac
     5881 cggttccctt tcgcggcaag cgcaacgagc ccgcttttgt cgtagccacc gcccgaaaac
     5941 tggctgaagt caaaggcgtt tctttggaaa ttattgccgc cgaaacaagc gcaaacgcct
     6001 tgcgcttatt cacgaaaatg cccgctccta cgaccggtgc ggaggcgggg tgagtgttac
     6061 ctttacaatt cttggttgcg gatcttcagg aggagttccg cggctcggac aaggctgggg
     6121 agcctgcgac cccaacaatc ccaaaaatcg acgccggcgc tgctcgctta tggtggaacg
     6181 ggccggggac aacggcgcca agacacatgt tctcgtcgac atgtctccgg atcttcgcga
     6241 gcaactgctc gggctcgaga tcagccgcct tgatgccatt ctgcttaccc attcgcatgc
     6301 tgaccatatc catggaattg atgacgtccg gcccttggtc atcaaggcgc ggcgaaaaat
     6361 cgaccttttc atggacgcgg ctacgtcagc cgttgtccgt accaacttcg gctacgtttt
     6421 tgagacgccg gccggcagcc aatatcctgc actcctaact gagtatcgtc tcgtgagcgg
     6481 ctattccata accataaatg gaccaggagg acctctcacc acgactccgt ttcagctgga
     6541 acatggggag attggcgcgc tcggctttcg attcggccaa attgcctata cccccgatgt
     6601 gaacgccatc cccccggaga gtgtagcttt cttggaggac cttgatgttt ggattgtcga
     6661 tgcactgcgg tatacgcctc atccaagcca tttttgcttg gacgaaacat tacattggat
     6721 taaaaaattg cgaccggccc gagccattct taccaatctt cattcggatc tcgattttga
     6781 ccgccttcgg agcgaactgc cggcaaatgt cgaaccggca tttgacggca tgcgcatttc
     6841 tgcttgaatc tgcagcgctt aagataatgt taccagctag gttttcgatg taagtgttca
     6901 ggatatttat agttacctac gataaattgc gtgttccata atatagatta tgcgaatcca
     6961 taaggagctg aaggctattg gtaaacgaac gtcgtattgg gttttccgat tggagtagcc
     7021 aaagcggctg acaattgcta tgctttcttg gtcacgggat tttcggcatt cggcatttgg
     7081 aacagcgaca atcggcgggc tttcgggctg gtacccttgg ggtgccggcg agcgtttgcg
     7141 tgcgcttttt ctggctgggc cgaggaactc gtgtttggca gcggggcgcg gcgccatgcc
     7201 atattctttg aagatttttt ggcggatacg aacaggcaca ccatgagcaa aggcaaagac
     7261 ttccgggggc cgaaaaagcg aggcttcgat gacgatgggc cctcacccta tgactctccc
     7321 cggatg
//
LOCUS       DATMPU010000219         3045 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_4499583,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000219 DATMPU010000000
VERSION     DATMPU010000219.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 3045)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3045)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3045
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4499583"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            82..351
                     /locus_tag="VNY06_05055"
     CDS             82..351
                     /locus_tag="VNY06_05055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX14212.1"
                     /translation="MFKLVDAPPASSTNSTPVSQIIEIPDVSLNEKLIPANDNYEPSA
                     LSVENLKAGSILAPRDYTKPGRSPLLIIALLCLVVLAAVIARLAI"
     gene            378..674
                     /locus_tag="VNY06_05060"
     CDS             378..674
                     /locus_tag="VNY06_05060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX14213.1"
                     /translation="MSGSSMPGRDLTDIRPERIGASQVEERNIAFPILAGIVGFALVV
                     GYAGYCFYTTAEMIKPYKKTNEKLGINLPRSEKATDRKSVQGEARPHPPRHLRT"
     gene            complement(671..1969)
                     /locus_tag="VNY06_05065"
     CDS             complement(671..1969)
                     /locus_tag="VNY06_05065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018320062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HWX14214.1"
                     /translation="MAMPVTRYAKSGDVHIAYQVFGSGATDLVFVPGFISHIENYWEH
                     PDLARWLLRLGAFSRVIMFDKRGTGLSDPVSELPSLDLRMDDVRAVMDAAGSESAALL
                     GVSEGGALAALFAATYPERCQRLALYGGFARFPIAGEVLKPLFKYIDQAWGRGLSLLA
                     FAPSRQNDPAMLQWWGRFERLGGSPAAVMAVVRMAAETDVSDILSSIRVPTLVIHCND
                     DTLIPSECGRFIARTIPGARLIELPGQDHLFFLHEQIGDCIEEFLTGSVSVAESHRVL
                     ATVLFTDIVGSTALAEQMGDRRWHDLLGAHHASVRRELARYRGSEVKSLGDGFMATFD
                     GPARAVRCACAIVEAVRPLEIQIRCGLHTGEIELAGRDVEGIAVHMASRVSALAGPGE
                     ILVSRTVKDLVAGSGLHFNEMGKHPVKGLKEPIEIYAASR"
     gene            complement(2798..>3045)
                     /locus_tag="VNY06_05070"
     CDS             complement(2798..>3045)
                     /locus_tag="VNY06_05070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HWX14215.1"
                     /translation="NGTATVSREDPLLARTVGAQLIVRVTARAIFPNCPCYIPTLQLI
                     DASIYAPRSGCEPPEPAWKDFDDFKDCVHPRQPTFKG"
BASE COUNT          674 a          892 c          859 g          620 t
ORIGIN      
        1 gcaattctcg gtttgtttag cgctagcgta tttgttgctc acgcgattga cgcgtatcgc
       61 gcctcatagg gtcgcccggc aatgtttaag ttggtcgatg ctcctccggc gagttccacg
      121 aactcaacgc cggtcagcca gataatagaa atacctgacg tgtcgctgaa cgagaaactc
      181 attccggcga acgacaatta tgaaccatca gccctaagcg tcgaaaatct taaggccgga
      241 agtattttag ccccgcgcga ttacaccaag ccgggcagaa gtcccctgct tatcatcgct
      301 ttgctctgtc tcgttgttct tgccgcagtc atcgctcgat tggcaattta gctcgaccgc
      361 cgcggcagtc tataccgatg tcgggatctt ccatgcctgg acgagactta accgatattc
      421 gaccggagcg cattggcgcc tcccaggtgg aagaacgaaa catcgcgttt ccgatacttg
      481 caggaattgt cggattcgcc ctggtggtcg gctatgctgg atactgtttt tatacgactg
      541 ccgaaatgat caagccttac aagaagacca atgaaaaact cgggattaac ctgccgcgct
      601 cggaaaaagc caccgatcgt aagtcggttc aaggcgaggc ccggccacac cctccgcgtc
      661 atcttcggac ctaacgagag gcagcataaa tctcgattgg ctctttgagc cctttgactg
      721 ggtgtttgcc catttcattg aagtgcaagc cagacccggc gactagatct ttgacagtcc
      781 gggacaccaa aatctcgcca ggcccggcaa gcgcggagac gcgtgaggcc atgtggactg
      841 cgatgccctc cacatcacgg ccagccaatt cgatctcgcc agtatgcagt ccgcagcgga
      901 tttgaatttc gagcgggcgg acggcctcga cgatcgcgca ggcgcagcga acggcgcgcg
      961 ctggaccgtc gaaggtggcc ataaatccgt ctcccagtga tttgacctcg ctgccgcggt
     1021 atcgcgcgag ctctcgccgc acactcgcat gatgagcacc gagcagatcg tgccagcgtc
     1081 gatcacccat ctgttcggcg agcgccgttg agccgacaat atccgtgaac aaaactgtcg
     1141 caaggacacg atggctctcg gcgacggaca ccgaaccggt gagaaattcc tcgatgcaat
     1201 caccgatctg ttcgtgcagg aaaaagagat gatcctgtcc gggcaactca atcagtcgcg
     1261 cgccggggat ggttcgcgca atgaaacgtc cacactcgct ggggataagc gtgtcatcat
     1321 tgcagtggat caccaaggtc ggcacgcgaa tcgaggagag aatgtcgctg acatcggttt
     1381 cggcggccat cctaacgaca gccatgaccg ctgcggggct cccgcccagt cgctcgaagc
     1441 ggccccacca ttgcagcatt gccggatcgt tttgacgcga gggcgcaaat gccagcaggc
     1501 taaggccgcg cccccaagcc tgatcgatat atttgaagag aggcttgagt acctcgccgg
     1561 cgatcgggaa gcgcgcaaaa ccgccgtaga gcgccagacg ctggcagcgc tcagggtaag
     1621 ttgcggcaaa cagggccgcc agcgcgccac cctccgacac gcccaacaat gccgcacttt
     1681 cactgcccgc agcgtccatg acagcgcgga catcgtccat gcgcaggtcc aatgacggca
     1741 actcggaaac ggggtcggaa aggccggtcc cgcgcttatc gaacatgatt acgcgggaga
     1801 aagctcccaa acgaaggagc catcgagcca aatcgggatg ttcccagtag ttttcgatgt
     1861 gcgagatgaa tcccggcaca aataccaagt cagtcgcacc acttccaaag acctgatagg
     1921 cgatatgaac atcgccgctc ttggcatagc gcgtcacagg catcgccatt taccctccga
     1981 tgctcagggg tgactggctc actccaaact agcgcacggc agctccggtg agaaggaatt
     2041 ataagcggag gatcgcaagg tcagcttggg atcattttca gacgtcagct tccgccgccg
     2101 cggttgaaac gactgtcgaa cgtttgatgc gccgccgcct ggtcggcttg gattgaaggc
     2161 caaagctgga tgtcagctcg tcccggcacg gtcctttacg gcgccctcgt cacgcgcgcg
     2221 ccagcgatga tgcctcaacc gccgccacat gagcgccgga ccgcgggcaa tgttattgcc
     2281 ggaatgatgg agaacctccg actgcaattg agctcgtctg atcatctgtc tcagacggca
     2341 tacaaccgcg gcagatgatc agttttcttc tcaatttatc gtcggctgcg cggttaatac
     2401 catcgagggc ggcagtccat tcctgcgagt atggttttag cctggaaagc tcatcctcgc
     2461 ttggagaact atctctagaa accacgtcgt cgcttgtctt tcgaactttt accggtttca
     2521 cgtggttacg tttcggaagg ggaccaagcc catcccagtt tgccagcgca gtggtcggag
     2581 gttggatgta gggcccgctg ccggcgcagc cgctgagagc aagccctgcc acgatcacaa
     2641 acaacaaccg tctactcatg gccaagactc gcgtatctcg aatccgcgcg gatggtttga
     2701 tagatagccc ggttgtggtt aattttttct tggtgcactg ttgcggcaga agcggaagtc
     2761 gcaatcgtta gcaatatgct tggcgagcgc taaaccgtca gcccttaaag gtcggttggc
     2821 gcgggtgcac gcagtcctta aagtcatcga aatccttcca cgcgggctca ggtggttcgc
     2881 agcccgaccg cggcgcgtag atcgaggcgt cgatcaactg cagcgtcgga atgtaacacg
     2941 gacaatttgg aaagatcgct cgcgcggtta cacggacgat aagctgcgcg ccgacggtcc
     3001 gcgcgaggag cggatcttcg cgactgaccg ttgccgttcc attga
//
LOCUS       DATMPU010000220         3639 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_2045341,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000220 DATMPU010000000
VERSION     DATMPU010000220.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 3639)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3639)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3639
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2045341"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            411..3053
                     /gene="pepN"
                     /locus_tag="VNY06_05075"
     CDS             411..3053
                     /gene="pepN"
                     /locus_tag="VNY06_05075"
                     /EC_number="3.4.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012383857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HWX14216.1"
                     /translation="MRTDTAKPVRLKDYRVPDYLIGKVDLDVKLHPSATKVQARLAIR
                     PNPQGRPNAPLVLDGDALIAKKIVLDRELLDLQEPAAWNGSATPDALTLNSPPQRPFD
                     LEIETEIDPSANTNLMGLYRSGSAYCTQCEAEGFRRITYFLDRPDVLSVYRVRLEAEI
                     SEAPLMLSNGNKIASGKIDGTARHFAIWHDPHPKPSYLFALVGGDLGSIFSEFTTRSG
                     RKVSLCIHVEHGKEPQATYAMDALKRAMAWDERVYGREYDLDVFNIVAVSDFNMGAME
                     NKGLNIFNDKYVLASPATATDIDYAHIEAVIAHEYFHNWTGNRITCRDWFQLCLKEGL
                     TVFRDHAFSADMRSAPVRRIADVRTLRAQQFAEDAGPLAHNVRPDTYMEINNFYTSTI
                     YEKGAEIIRMLKVLIGDDAFRKGMDLYFDRYDGTAATIEDFISCFAEISGRDLTQFSR
                     WYNQSGTPLLEATASYDEVTKTCTLDFSQSCAPTPGQEKKEPFVIPIALGLVAPGFGD
                     IALRAGGNDGASAAELSSGVIELSTLRRRVTFRDVPCRPVPSLLRGFSAPVRLEFAAS
                     EADLMTQISHDSDSFNRWQAAQTYATRLLRRSAARIRAGDAPEHDGDFIAALGRIIDQ
                     TTDPAFTAQAITLPSESDIAREIGQDVDPDAIHLAREALRQGIGRGTKEALLAAYKRL
                     SADGPYSPDAVSAGRRALRNSALDLLAAGEKVEGTRLAKRQFEDAETMTDKFAALCVL
                     AQNPGGEREAALDSFYGAYEGEPLVIDKWFALQATIPERRTLNRVKVLMKHKAFSSSN
                     PNRLRALAGAFAAGNPTQFNADDGSGYDFIAGLVLDLNAKNPQVAARLLSAFKSWRTL
                     EPRRRALAESALRHVASAAALYRI"
     gene            3019..3519
                     /locus_tag="VNY06_05080"
     CDS             3019..3519
                     /locus_tag="VNY06_05080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX14217.1"
                     /translation="MWPARPRSIGFDKRAERNSDEILEATFVDSAPLFDLLATKNSQV
                     IYGRRGTGKTHALKYLARHVEKTNERPIYIDLRYVGSNGSIYSDGSRPLAERASTLIL
                     DVLNAINGELYVLAVEALDKAPDPDQITLRLTISPTQYKASESPAQLRQKTEEKILGR
                     KTIRLA"
BASE COUNT          824 a         1083 c          954 g          778 t
ORIGIN      
        1 tcatgtcgtc aaaatcggct attcccgccc acacctttgc gggtggcgcg tcgatcccaa
       61 tcgactcggt aattttctgc cacgacggcc catgcgccca tgccgcaggg gatagcaaaa
      121 gggcgaagag cgcaatcgcg ccaagaaaag acttttgggc tattccaccc atgcctatat
      181 ttcctctctt cctttagttc ttgctgtcat gctaggcgca aactagccgc gctgagctgg
      241 cgcctcccct gcggaaaagt tttggcaacg ccgcaagaaa agatcttccc gccgggcatc
      301 gctgccgtga tagtttgctt tgcggcttgc aaacggtcct cctatgattg ccgcttccgg
      361 tcatgcgcgg gagagctccc tccccgcgcc tgtttgccaa gaggtataca ttgcgcaccg
      421 ataccgccaa acccgtccgt ctcaaggact acagggttcc tgactatctg atcggcaagg
      481 tcgatctcga tgtcaagctt cacccttcgg caacaaaagt tcaggcgcgc ctcgcgatcc
      541 gtccgaaccc gcaaggccgg cccaacgccc ctctcgttct tgatggcgat gcgctcatcg
      601 caaaaaagat cgtgctcgat agggagctcc tcgatctgca agaacctgcg gcatggaatg
      661 gctctgcgac gcccgatgcg ttaaccctga attctccgcc gcagcgccct tttgacctcg
      721 aaatcgagac cgaaatcgat ccttcggcca ataccaatct catgggcctc tatcgctcag
      781 gctccgccta ttgcacgcag tgcgaggcgg aaggctttcg ccggatcacc tattttctcg
      841 accgccccga cgtgctgagc gtctataggg tgcggctgga agcggagata tcagaggcgc
      901 cgctaatgct ctcgaatggc aacaaaatcg cgtctggaaa aatcgacgga acagcgcgcc
      961 atttcgccat ttggcacgac cctcatccaa agcctagcta tctttttgct ttggtaggcg
     1021 gcgatcttgg ctccattttc agcgaattca caacgcggtc cggccgcaag gtttcgctct
     1081 gcatccatgt cgagcacggc aaggagcccc aagccacata tgcgatggac gctctaaaac
     1141 gcgctatggc ctgggacgag cgcgtctatg ggcgcgaata tgatcttgat gtttttaaca
     1201 tcgtcgcggt ctcggatttc aacatgggcg cgatggagaa caaggggctg aacattttta
     1261 atgacaaata tgttcttgcc tcgcccgcga cagcaacaga catcgattat gcgcatatag
     1321 aggcggtcat tgcgcatgaa tattttcaca attggaccgg gaaccggatc acctgccgtg
     1381 actggttcca gctctgcctc aaggagggtc tcactgtttt ccgcgaccat gcattctcgg
     1441 ccgacatgcg ctcggcgccg gtgcgccgga ttgccgatgt ccgcacattg cgcgcgcagc
     1501 aattcgccga ggacgcgggg ccgctcgcgc ataatgtccg gccagacact tatatggaaa
     1561 taaacaattt ctacacctcc acgatctatg agaaaggcgc cgaaatcatc cgcatgttga
     1621 aggtgctcat tggcgatgat gcctttcgca agggaatgga tctctatttt gaccgttacg
     1681 atggaacagc ggcgacgatt gaggatttca tctcgtgctt tgctgaaatc tcaggtcgag
     1741 acttgacgca attctcgcgc tggtacaacc aatcgggcac tccactctta gaggccacgg
     1801 cgtcttatga tgaggttacg aaaacctgca cgctcgattt ttctcaatcc tgcgcgccaa
     1861 ccccgggaca agaaaagaag gagccctttg tcatccctat tgcgctcggc ctcgtcgctc
     1921 ccggctttgg ggacatcgca ttgcgagcag gcggcaatga tggtgcaagc gcggccgaac
     1981 tttcaagcgg cgtaatcgaa ctttctacct tgcggcggcg agtcactttt cgcgatgtgc
     2041 catgccggcc ggtcccttct cttctccggg ggttctcggc gcccgtccgg cttgaattcg
     2101 cggcaagcga agcggatctc atgacgcaga tctcccatga cagcgatagt ttcaaccgct
     2161 ggcaagccgc acagacctat gccacgcgat tgctccggcg atctgcggca cgtatccgcg
     2221 ccggagatgc acccgaacac gatggggact tcatcgccgc gctgggaagg ataattgatc
     2281 agaccacaga tccagccttc accgcacaag cgatcacttt gccgagtgag tctgacattg
     2341 cgcgcgaaat cggccaggat gtggatcccg atgcgatcca cctcgcccgc gaagcgctgc
     2401 gccaaggaat aggacgaggt accaaagagg ctttgctcgc ggcttataag cgtctctccg
     2461 cggacggtcc ctattcgcca gacgctgtct ccgccggacg ccgggccttg cgcaactcag
     2521 cgctcgatct tctcgccgcc ggggagaagg tcgaaggtac ccgtcttgcc aagcgtcaat
     2581 tcgaggacgc cgaaacaatg acagacaaat tcgccgcgct ttgcgtgctc gcacaaaatc
     2641 caggaggtga acgcgaagct gcactcgatt ccttttatgg tgcctatgaa ggcgaaccgc
     2701 tcgttatcga caaatggttt gccttgcagg cgactatccc cgaacgacgc acgctcaacc
     2761 gggtgaaggt gcttatgaaa cataaagcat tttcgtccag caaccccaac cggctgcgcg
     2821 cgctcgctgg cgcattcgcc gccggaaatc caacccaatt caacgccgat gatggcagcg
     2881 gctatgattt tattgccgga ctcgtcctcg atctcaatgc aaagaatccg caggtggcgg
     2941 cgcgccttct ctcggccttc aagagctggc ggacgctaga gccgcgccgc cgggccctcg
     3001 ccgaaagcgc cttgcgccat gtggccagcg cggccgcgct ctatcggatt tgataaacga
     3061 gcggagcgaa actcagacga gatattagaa gcaacgttcg ttgattcggc tcctcttttc
     3121 gaccttttag ctaccaaaaa tagccaagta atatatggca gacgtgggac cggaaagacg
     3181 catgcgctga agtacctcgc tcgtcacgtt gaaaagacaa acgaacggcc aatttacata
     3241 gatcttcgtt atgtaggctc taatggttca atttacagcg acggtagccg cccgcttgcc
     3301 gaacgcgcct caactctaat cttggatgtc ctgaacgcca tcaatgggga gctgtatgtt
     3361 ttagcagtcg aagctctaga taaggctccc gatcccgatc agatcactct gcgcttgacg
     3421 atttctccaa cgcaatacaa agcgtcagaa tcaccggcac aattgagaca gaaaaccgaa
     3481 gagaagattc tcgggcgaaa gacaataaga ttggcatgaa actcggtcac caggcgatgc
     3541 caagcctcac cgtagcaatc agctcaaaaa cagccgaagc caccaactcc tccttacaaa
     3601 cgactagggt caggacctat taactgaagc gtgattcaa
//
LOCUS       DATMPU010000221         4406 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_3586843,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000221 DATMPU010000000
VERSION     DATMPU010000221.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 4406)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4406)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4406
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3586843"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..893
                     /locus_tag="VNY06_05085"
     CDS             <1..893
                     /locus_tag="VNY06_05085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012589733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HWX14218.1"
                     /translation="FVAQIFARTHWMRALMRGPVQLFQAIALLRMELPIRSRAPISHR
                     SLGLSRLIDLLWLAIIALGTIWALWLIKDYIRHELGWGEVWKSGLYTLFTMARVILLM
                     VLASLVWVPVGVWIGLRPAIAEKAQPLAQFLAAFPANVLFPVAVVLILRFSLNPDIWL
                     SFLIIFGTQWYILFNVIAGASAFPNDLKEAVANFRIRGFSWWRNVIIPGIFPYYVTGA
                     LTASGGSWNAAIVAEYVKWGSDVVSAHGIGAYIAKATADGDYPRIVLGVAVMSIFVIM
                     FNRLLWRPLYRLAEKRLRLD"
     gene            903..2237
                     /locus_tag="VNY06_05090"
     CDS             903..2237
                     /locus_tag="VNY06_05090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018427250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/sulfonate/bicarbonate ABC transporter
                     ATP-binding protein"
                     /protein_id="HWX14219.1"
                     /translation="MQVNNGRSSTQGTKPLLEVRNVSQRYQTGAGDEGPAVLDNISLD
                     LKVDEIVGLLGRSGCGKSSLLRIVSGLIFPSAGEVRYLGEHVHGPVDGTAMVFQSFAL
                     FPWLTVLANVELGLRAKQIAKDEARKRALKAIDLIGLDGFESAFPKELSGGMRQRVGF
                     ARALVVHPSILLMDEPFSALDVLTAETLRTDLLDLWVEGRMPIKSILMVTHNIEEAVL
                     MCDRIIVLSSNPGRIAAEIPVKLQHPRNRLDPEFRQLVDKIYALMTKRLETAVTSREC
                     AFPGLGLAMTLPRVSTNTLAGMIEEIAAEPYLGRADLPALADSLQMEIDELFPIAESL
                     QLLRFAELEEGDIKLTASGRRFAEADVDVRKKLFGDHLLSYLPLAAHIKLVLDERPSH
                     TARAVRFLEELEDYMSEDYADRTLKSVVNWGRYGELFAYDETSETFSLENPQ"
     gene            2594..3937
                     /locus_tag="VNY06_05095"
     CDS             2594..3937
                     /locus_tag="VNY06_05095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012558189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HWX14220.1"
                     /translation="MQSSGGSIFDFLGIVAILFLVIANGFFVAAEFSLVAVRRSRVAE
                     LVADGRMNASTLQWAINHLDSYLAATQLGITISSLALGWIGEPALAHLIEPCLSALPG
                     SLAVASAHAIAVAFTFITITALLVVLGELAPKNLALQRSEGTALWVVRPLGLFLFLFR
                     PAIIILNGLGRLVLKTAGLQPGAAMESLHSPEELKFLVQASQEGGILHQAQHEVVVRA
                     LNIGERPIGYIMTPRPEVEWIDTNDRREEILRAIRECRHQQLLAGRGSIDEPLGVVSK
                     KDLLDQVLDGQALDPMAVIREPLVVLESTPTFKVLERFKQGPVRLAIIVDEYGSLQGI
                     VTQTDLLEAIAGDLPDVEGEEPNIIEREDGSLLIDGLMPAHDAFDHLGVKTRPEDGDF
                     HTIAGFALSELGHLPEAGEAFTYEGWRFEIVDMDGLRIDKLLAQREPANEQESMP"
     gene            4133..4209
                     /locus_tag="VNY06_05100"
     tRNA            4133..4209
                     /locus_tag="VNY06_05100"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4167..4169,aa:Trp,seq:cca)
BASE COUNT          847 a         1291 c         1257 g         1011 t
ORIGIN      
        1 ccttcgtggc gcagatattc gcaaggaccc attggatgcg ggctttgatg cgcggcccgg
       61 tgcaactgtt ccaagctatc gcgcttctgc gtatggaact gccgattcgc tctcgcgcac
      121 cgatttcgca ccgcagcctc ggtttaagcc gcctgatcga tctcctgtgg ctcgccatca
      181 ttgccctcgg cacgatttgg gctctttggc tcatcaaaga ctacattcgt cacgagctcg
      241 gctggggcga agtctggaaa agcgggctct acactctctt tacaatggca cgcgtgatct
      301 tgctcatggt gctcgcaagt ttggtttggg tacctgtcgg cgtttggatc ggtctaaggc
      361 ccgcgattgc ggaaaaagcc cagcccctgg cgcaattcct ggcagcgttt ccggcgaatg
      421 tcttatttcc ggtcgctgtt gttctgatcc tcagattttc cctcaatccc gacatctggc
      481 ttagctttct gatcatcttt ggcacgcaat ggtacattct ctttaatgtt attgcaggtg
      541 cctcggcttt tccgaacgat ctcaaggaag cagtggcgaa ttttcgcatt cgcggcttct
      601 cctggtggag gaacgtaatt attcctggaa tcttccccta ttatgtgact ggcgcgctga
      661 ccgcatccgg tggatcatgg aatgccgcaa tcgtggcgga atatgtgaaa tggggcagcg
      721 acgtggtgtc ggcacatggg atcggcgcct atatcgctaa ggcgaccgcg gacggcgact
      781 atcctagaat cgtgcttggc gtcgcggtga tgtcgatctt cgtcattatg ttcaaccgcc
      841 tgctgtggcg cccgctttac cggctcgccg aaaagcgctt acggcttgat tgaggtgatt
      901 tcatgcaagt taataacggg cgaagttcaa cccaaggcac taagcccctc ctcgaggtca
      961 gaaacgtctc ccagcgctac cagacgggcg cgggcgatga gggtccggcg gtccttgaca
     1021 acatttcgct cgatctgaag gtggatgaga tcgtcggcct attggggcgc tcgggctgcg
     1081 gcaaatcctc gctcctgcgc atcgtatcgg gcctcatttt tccctccgcg ggcgaggtgc
     1141 gttatctcgg tgaacacgtg catggtcccg tcgatggcac tgccatggtg ttccagagct
     1201 ttgctctgtt tccctggcta accgtgctcg ccaacgtcga acttggcctc agggctaagc
     1261 aaattgccaa ggacgaagcg cgcaagcgcg cgctcaaagc aatcgacctc atcgggctcg
     1321 acggtttcga gtccgcattt ccgaaggagc tgtctggcgg catgcgccag cgcgtgggct
     1381 tcgcgcgggc gcttgttgtc caccccagta ttcttttgat ggacgagcca ttttccgcac
     1441 tcgacgtgct taccgcggaa acgttgcgca ccgaccttct cgatctctgg gtcgaagggc
     1501 gcatgccaat aaaaagcatc cttatggtca cccacaatat tgaagaagct gtattgatgt
     1561 gcgaccgcat catcgtgcta tcaagcaatc cggggcggat cgcggccgag attcctgtca
     1621 aattacaaca tccccgcaac cggctcgatc ccgagttccg ccaattggtc gacaagatct
     1681 acgcgttgat gacaaagcgc ctggaaacgg cggtaacgag ccgcgagtgc gcattcccag
     1741 gtctcggttt ggcaatgaca ttgccgcgcg tttcaaccaa tacgcttgcc ggcatgatcg
     1801 aggagatcgc cgccgagccc tacctgggcc gcgccgacct cccggcgctc gccgacagtt
     1861 tgcaaatgga gatcgacgaa ctgttcccca tcgccgaatc cttgcagctt ttgcgcttcg
     1921 cggaactcga ggagggagat ataaaactga ctgcttccgg acgccgtttc gctgaggcgg
     1981 acgtcgatgt ccgcaagaag ctcttcggtg atcatctcct ctcctatctg ccactcgctg
     2041 cgcacattaa actggtgctc gatgagcggc caagccacac ggcccgcgcg gtacgcttcc
     2101 tcgaggagct cgaggactat atgtctgagg actatgccga tcgcactttg aagagcgtcg
     2161 tgaactgggg ccgttacgga gagcttttcg catatgatga aacctccgaa acctttagcc
     2221 tggagaatcc gcaatagggc cgcgatggct ccagccgaaa ggccccggca cgttgcaccg
     2281 tttgaatttg tgcagtgcga atgggctgcc gaaggaaaac gcccatcgag cgtagccgca
     2341 tgagcgagat agctaattct cggcaccgaa atgacccatt cgcgcttggg ctcctttgca
     2401 gtaaagtatc gccgagcggc gcgccgtcat ttggcacggg cgctccgtgc cgggccgatt
     2461 ggcaacctca ttcctgccgc tcccgcggct tatgcgactc gatcctcggc ccctgcgggc
     2521 aacgttggcg taggaaccaa agtcttatac ccgacacccg cctacgactg cgcaaggcgc
     2581 agggaaggag accatgcaaa gttctggtgg cagtattttc gatttcttgg gaatagttgc
     2641 cattcttttt ctcgtcatcg ccaacggatt tttcgtcgcc gcggaattct cactcgttgc
     2701 cgtgcgccgc agccgtgttg cggaacttgt ggcagacgga cggatgaatg cgtccacgtt
     2761 gcaatgggcg attaaccatc tcgattccta tcttgccgcg acccagcttg ggatcacgat
     2821 ttcctctctg gcgctcggct ggattggtga gcctgcactg gcccatttga ttgagccctg
     2881 tctgagtgca ctgcctggat cgctcgctgt cgccagcgcg catgccatcg cagtcgcatt
     2941 caccttcatc acgatcacgg cgttactcgt cgtgctggga gagctcgcac cgaaaaacct
     3001 cgcactccag cgcagcgaag gcactgccct ttgggtcgtg cgcccgctcg ggctctttct
     3061 gttcctgttc cgccccgcga tcataattct taacggcctc ggcaggttgg tactcaagac
     3121 cgccggattg caaccaggcg cggccatgga atcgttgcac tcgccggagg agctgaagtt
     3181 tttggtccag gcaagccagg aaggggggat ccttcatcag gcgcagcacg aggttgtggt
     3241 ccgagccctc aacatcggcg agcgccccat cggctacatt atgactcccc ggccggaagt
     3301 ggagtggatc gacaccaacg accgccgcga ggagatcctg cgggccatcc gcgaatgccg
     3361 ccaccagcaa ctcctcgccg gcaggggcag cattgatgag ccgctgggcg tggtctcaaa
     3421 aaaagatctt ctcgaccagg ttctcgacgg acaggcgctc gatcccatgg cggttatccg
     3481 cgagccgctt gttgttctcg aatcgacacc gacgttcaaa gttttggagc ggttcaaaca
     3541 gggcccggtg cggctcgcga ttatcgtcga tgaatatggg agccttcaag gcattgttac
     3601 acaaaccgac ctgctcgaag ccattgctgg cgatctgccg gacgtcgaag gcgaagaacc
     3661 gaatattatc gaacgggagg atggctcgct tttaatcgac ggcctgatgc ccgcgcatga
     3721 tgcgttcgat catctcggcg tgaagactcg gccggaggat ggcgacttcc atacaatcgc
     3781 agggtttgcc ttgtccgagc ttggccatct gcccgaggcc ggagaggcct tcacctatga
     3841 aggctggcgt tttgagatcg tcgatatgga cggcttgcgg atcgacaagc tgctcgccca
     3901 aagggagcct gccaatgaac aagaaagcat gccataggct tgccggcaat cgccattgcc
     3961 gtttcccagc aggggaccgt gcagatggcc tttttgggcg cggaccgtct gctcacttct
     4021 accgtcgggc gactttctca tggaatccct tgcaccgggt tttcggctgc gctcgcaaga
     4081 tccgccgccc ttgcacgggt aagtcggttc acgctaaaag cttcgcgcgt gcaggagtgt
     4141 agctcaattg gttagagcac cggtctccaa aaccgggggt tgggggttcg agtccctcct
     4201 ctcctgccaa taaatcaaag acttacgaaa aatgcggcgg ttaaggttga cacttttaac
     4261 agctaaggtt gacagttatg ttcgcctttt ggccagttcc tcgtcgaagc ttttgacgac
     4321 tccagccatc tttccgcgta gatcggcgcc cttggcatag tgccgcgcca tttcgatagt
     4381 tttctgcccg agcgcgtcgg caatcg
//
LOCUS       DATMPU010000222         3814 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_3890500,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000222 DATMPU010000000
VERSION     DATMPU010000222.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 3814)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3814)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3814
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3890500"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            132..455
                     /locus_tag="VNY06_05105"
     CDS             132..455
                     /locus_tag="VNY06_05105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX14221.1"
                     /translation="MIDRRLPPEERHSPEPEIIPPGNEDGSEAENPVWGRGTIEEHSI
                     HRIYVTKVGPFGLLPLMLLGGVIAIAMLAFLFGFLLIFIPLVGLILAAALIGRFLRGG
                     PRWPR"
     gene            complement(504..1394)
                     /locus_tag="VNY06_05110"
     CDS             complement(504..1394)
                     /locus_tag="VNY06_05110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012385952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HWX14222.1"
                     /translation="MDEWDFRLTPARPDLAAVHLRGLVAADAYVEGRTMHVITGVADL
                     RRAPSHEALLETQALFGEEVIGYEDREGWGWVQLAGDGYVGYMPLAALADGRIEPTHR
                     VAVNRTFVYQCPDIKMSARRSSPLGSAVRVEAREGLFAKIGPNAFVLADHLRPYSERE
                     NDFVAVAERFLHVPYLWGGKTSLGIDCSGLVQLSLYVTGIAAPRDTDLQEKALGEPLA
                     VDAQLNGLQRGDLVFWRGHVGIMQDEANLLHANAHHMLVASEPLRIARERILAQTSKP
                     ISAIKRLKGGTNWDTRQDKI"
     gene            complement(1469..1981)
                     /locus_tag="VNY06_05115"
     CDS             complement(1469..1981)
                     /locus_tag="VNY06_05115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX14223.1"
                     /translation="MGSGCKAKVLAIVAAFASVSNEAQSAPSPSKAHHHYRQASGSLS
                     RTYIRGLSHGGLAYARRAYWTSQDRGTGYVNLVGDPGSGLGFYALPLKYRIGAWRYRL
                     THRRPWWHNPVLFAVAADAARYNYWIPANHDYAYGVFNPYDGVGTPFFGGYYGGSRDD
                     ETPSDFYGWA"
     gene            3385..3558
                     /locus_tag="VNY06_05120"
     CDS             3385..3558
                     /locus_tag="VNY06_05120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX14224.1"
                     /translation="MTVRVKDPLNDGQIYTQHRKTASDASPDIVQDPGARRSPPLPFA
                     MMLSSRVTEQGTS"
     gene            complement(3577..>3814)
                     /locus_tag="VNY06_05125"
     CDS             complement(3577..>3814)
                     /locus_tag="VNY06_05125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX14225.1"
                     /translation="IRACSEGNPRFAENIKSIMEKIMPFKAGTTNDADGGVGTSGSGR
                     GLLILRGQPVQERSLVLVSDVSKPDDQQDSVRQY"
BASE COUNT          847 a         1086 c         1043 g          838 t
ORIGIN      
        1 tgccggagca cttttagctg gcgccgttcg tgtttgcggc ctaaagcgag atgacgcgaa
       61 agtgacgcgc aaagaatttc ttgccgctgt gcgccaaccc caagctttgc acaagctttg
      121 cacaagataa aatgatcgac cggcgtttac cacctgaaga gcggcattcc cctgagccgg
      181 aaatcatccc gcccggcaat gaggacggca gcgaggccga aaaccccgtc tggggccgag
      241 gaacgatcga agagcatagt atacaccgca tctatgtgac caaggttgga ccgtttggcc
      301 ttctgccctt aatgttgcta ggcggtgtca tagcaatcgc aatgctcgcc tttctgttcg
      361 gctttctttt gatttttata cccttggtag gcttgatcct ggccgcggcc ctgatcggcc
      421 gttttttgcg cggcgggccg cggtggccgc gctgatatcg ccgcctagcg caagatgtct
      481 ctccttcgag ttgtcatctt gctctaaatc ttgtcttgtc gcgtgtccca attagtacct
      541 cctttaagcc gcttgatcgc ggaaattggt tttgacgttt gagccaaaat tctctcccgc
      601 gcgatccgca gaggttcgct cgcaacgagc atgtgatgcg cgttggcgtg gagtaaattg
      661 gcttcgtctt gcataatgcc gacatggccg cgccaaaata cgagatcgcc gcgttgtaag
      721 ccattgagct gcgcatcaac agcaagaggc tcgcccagcg ctttttcctg cagatcggta
      781 tcacgcggcg cagctatccc ggtcacatag agagaaagct gcacgaggcc tgaacaatcg
      841 atgccgagac ttgttttacc gccccagaga taaggcacat gcaagaaccg ctcggcgacg
      901 gcaacgaaat cattttcgcg ctcactgtag ggccgcaaat gatccgcaag gacaaaagcg
      961 tttggtccga tttttgcgaa aagaccttcc cttgcctcaa cccgcactgc ggaacctagc
     1021 ggcgaagacc tccgcgcgga catttttatg tccggacatt gataaacgaa ggtccggttg
     1081 accgcgacgc ggtgggttgg ctctatgcgt ccgtcggcga gtgcggccaa gggcatatag
     1141 ccgacatatc cgtcacccgc gagctgcacc cagccccaac cttcgcgatc ttcgtaaccg
     1201 atcacttcct cgccgaagag cgcttgggtt tcgagcagag cctcatgtga gggcgcacgc
     1261 cgcagatcgg caacgccggt tatgacatgc atcgtccggc cttcgacgta agcatcggcc
     1321 gcaaccagtc cgcgtagatg cacggctgca agatcgggcc tagccggtgt caagcgaaaa
     1381 tcccattcgt ccatgccgat gaattatccg ctttgcgtaa acaaagctac ggctgaactt
     1441 aaactttcgc cggcgctacg tagcaagctc aagcccagcc ataaaagtcc gacggcgtct
     1501 cgtcatcgcg agacccgcca taatacccgc cgaagaaggg agtacccacc ccgtcgtacg
     1561 ggttaaagac gccgtaagca tagtcatggt tggccggtat ccagtaattg tagcgggctg
     1621 cgtcggcggc gaccgcgaag aggaccggat tatgccacca ggggcgccgg tgggtcaggc
     1681 gatagcgcca ggcgccaatg cgatatttta gcggcagcgc ataaaagcca agcccgctgc
     1741 cgggatcgcc gacaaggttc acatagcccg tccctcggtc ttgcgacgtc caataggcgc
     1801 gccgcgcata ggcaaggccg ccatgactaa ggccgcggat ataggtccgc gagaggctgc
     1861 cgctcgcttg ccgataatga tgatgcgcct tggaaggcga gggtgcgctt tgagcttcgt
     1921 ttgataccga cgcgaaagcg gcaacaatcg cgagaacttt tgccttgcag cccgagccca
     1981 ttttctaatt ctccggaata tgcggtatta atttcgatac ttgacgataa acgggtgatc
     2041 catccttgcg gttccattgc cattgagcag cattcaccta ttgaggatgg cgcctgccgg
     2101 atgccggccg tagccgtgct ggcgcgtggc acgcggcctt tgacatagga tcccgcagcc
     2161 aaatttacgt cggtcgtgtc aggaggcgat atttggcccg cgaatccacc gtgggatcat
     2221 gaggcgtaca ttcctgacaa acagtgcgct ccagcgataa atttagacct ttccttcatc
     2281 caatctttct tttgagatga tataagacaa gtgaacggcc gctgcggtcg aaggactgct
     2341 gctttgcggg ggtcaaacca tcgtaaagtt agtggcagtt ttgcggaatt ctcaggattc
     2401 gctgaggaca ggcttatcgg cttgagcctc gaacactgct ttctagagga gaactcgcgc
     2461 ctgaaactgg ttcaacatcc caacggccga tcgaaagtga cttgcgcggc gctgacggat
     2521 cgatgatacc catcgggcga ttgccgtccg ggatcttagg gcgcaggccg gtgttcgtgt
     2581 gccacccctc tgttgaagga gtttccaatg caaaccatga aacaattgac gaatcagccc
     2641 caaacccctc tcaataggcc attttaagga gcgaagttgg ggagctgcac tagggattag
     2701 gacctttagg aagaacgatg acgccacatg cacgtcgtgc ctctggaagc attcgatcag
     2761 acgattgctc agccttgtca ggttggtaaa cacttcgata aaggtgaaac ctttaacgcg
     2821 ggcttgagct ggatttgccg gcacccgagc tccgccgcct cgtcgaggac gcccgtccaa
     2881 tcgcgagtac tcattgctag ccgagggccc gctttcggcg tagcagtgtg agcatgtaag
     2941 gttgcaccgg ccagtgattt ccagccaaag gaatgctaaa catccaacgc gtcaattgtg
     3001 gcttcatatg ctgccggaga catgccgctg ctccatgagt cgtcgatcag agtgcacaag
     3061 cttcaagaat atctacgcgg aaaccgaaac gcggcgcaat agtcacggaa gcgatcaaga
     3121 agagggctgt caaagcggtt cgacgcatgc tacctcgggc aatcacccgt tcacatgggc
     3181 tggcccgttg ctggattaaa gtgtcagtgc ccgtcagccc tacgatctat gcggacagca
     3241 atagatttac ttagttatcc acataacaca ggcttgataa aatttcaccc gattgacctt
     3301 gcggcgcgga tatgccgttg agcgattcgt ccatggccat tccactttcc aacggatcca
     3361 aattttaaac tgaggcacta ccgcgtgacg gttagggtta aggatcctct caacgacggg
     3421 caaatttata cccaacatcg caagaccgct agcgacgctt cgccggatat cgtgcaggat
     3481 cccggcgcca ggcgttcgcc gccattgccc tttgcaatga tgctatcaag ccgggtcact
     3541 gaacagggga cgtcgtgagc ctatcgcgca ctttcatcag tactggcgca cactgtcttg
     3601 ttggtcgtca ggtttgctca cgtcggagac aaggaccagg ctgcgttcct gaaccggctg
     3661 acccctcagg atcagcaacc cacgaccact accactcgtc ccaacgccgc catcggcgtc
     3721 gttggtcgtt ccagctttaa acggcatgat tttctccatt atcgacttga tgttttccgc
     3781 gaacctggga ttgccttccg aacaggcccg gatc
//
LOCUS       DATMPU010000223         4644 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_1718587,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000223 DATMPU010000000
VERSION     DATMPU010000223.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 4644)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4644)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4644
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1718587"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(26..862)
                     /locus_tag="VNY06_05130"
     CDS             complement(26..862)
                     /locus_tag="VNY06_05130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020176393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HWX14226.1"
                     /translation="MRTLFCKNAENFHTCSRPLALCFAACLLLDLAILPPCLAAGPSQ
                     AVRRIVRIASEGARPPYNYLENNKLAGFEIDLAQDLCSRMKVSCVFIAQNWDGLIPGL
                     LAHQYDAIMAAMEITDEARQKIAFSKPYIRMPSAILTLKGHATLDTSPAGLVGKNIGV
                     LSGGSHQAYAEDTYKQSEIHPYASLEEAILDLGEGRVDLVIGDKDAIVSFMRNRKEGE
                     CCIIGADVPHDPAYFGDGIGIGLRKEDKSLKAMFEKALDSSIADGTFARIKAKYFDFK
                     IN"
     gene            1086..2159
                     /locus_tag="VNY06_05135"
     CDS             1086..2159
                     /locus_tag="VNY06_05135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012384771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HWX14227.1"
                     /translation="MVSDQDVLAALQKVPGPDGKTPLTSSGVLSGLSIKDSKVYVSIS
                     VDPRHSGAFEPMRAAAEAALRRLPGVTLALVSLTAEKQAPPKPISSRGVAIPGISNII
                     AVASGKGGVGKSTCCVNLALGLVSLGWRVGILDADVFGPSLPRLLGLKAKPESEGRSI
                     RPLVAYGVKAMSIGFLVEEEDAMIWRGPMVMSAIQQLLRDVLWGELDCLLVDMPPGTG
                     DAQLTLAQNVPLAGAIIVSTPQDLALIDARRGIAMFNKVAVPVLGIIENMSYFLCPHC
                     GARSDIFAHGGARIEAEKCAVPFLGEVPLDIGIREHSDSGRPLTAVDPDGQNAKIFRT
                     IAAKVKAALETGRTRAAPKITIE"
     gene            2703..4280
                     /locus_tag="VNY06_05140"
     CDS             2703..4280
                     /locus_tag="VNY06_05140"
                     /EC_number="3.4.17.19"
                     /inference="COORDINATES: protein motif:HMM:NF014165.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HWX14228.1"
                     /translation="MHNDGKAKAESGAEMEQAPAAKPARQPSAEAKLGELKRRLLEIS
                     DLEAVGSVLGWDHATYMPTGGAAARARQGAMLSSLRHERSVDLTLGKLLDNLLPHAEG
                     LRYDSDEASLIRVARRDFEKATRLPADYVARASALSSASYDAWTRARPANDFAAMQPF
                     LEKAVDLAREYASYFAPYENVADPLIDSAEEGMTAAMIIELFARLKRHLQPIVGAICD
                     QAVPCDDCLHGFFSEAAQLDFSLSVVKRFGYDTNRGRLDKTHHPFCTKFSLGDIRITT
                     RVNERQFGSALFAAMHEAGHAIYEQGVAPALEGTPLGSGASWGVHESQSRLWENLVGR
                     GRPFWEHFYPVLRNEFPDQLRGIDDETFYRAINKVQRSLIRTDADEVTYNLHIMMRFD
                     LELELLEGRLRVKDLPEAWRARMKTDLGIAPDDDRDGCLQDVHWYGVGTVGGGFQSYT
                     IGNILSAQFYDAAMKAHPEIPNEIANGEFGTVHGWLRTHHLPTWAQVWARRARLSCGR
                     RAYEHRTLHRISSPEVR"
BASE COUNT          970 a         1222 c         1385 g         1067 t
ORIGIN      
        1 tttccgcctt cagtgtgcgg ggcggctaat ttattttgaa atcaaaatat ttggctttga
       61 tcctcgcaaa tgtgccgtcc gcgatgcttg agtccaaggc tttttcgaac atagccttca
      121 gggatttgtc ctccttgcgc agaccaatgc caataccgtc gccaaaatag gctggatcgt
      181 gcggcacgtc cgcgccaatg atgcagcatt ccccttcctt acgatttctc atgaagctga
      241 caatggcatc tttatctcct atgacaaggt cgacacgccc ttcgccgaga tcaagaattg
      301 cctcttcgag agacgcatag ggatgaatct ccgactgctt gtaggtgtcc tctgcatagg
      361 cttggtggct gccgccagat agaaccccga tgttcttccc gacgaggcct gcgggactcg
      421 tatcaagcgt tgcatgcccc ttgagggtga gaatcgccga gggcatgcgg atgtaaggtt
      481 tgctaaaagc gattttctgg cgcgcctcgt cggtgatttc catcgccgcc atgatggcat
      541 cgtactgatg ggcgagaaga ccgggaatga gaccgtccca attttgggca atgaaaacgc
      601 aggaaacctt catgcgtgag cataaatcct gcgcgagatc aatttcgaaa ccggcaagtt
      661 tattgttctc caggtaattg tagggtgggc gcgcaccttc gctcgcaatt ctaactatcc
      721 gcctcacggc ttgggaagga cccgcggcga ggcaaggcgg cagaatagca aggtcgagga
      781 gcaagcaggc agcaaagcag agtgccagcg gccgggaaca ggtgtggaaa ttctcggcat
      841 tcttgcaaaa tagcgtcctc attgcgctcc tatcttaagg ccgcaggctg ggttgattgg
      901 aggcaggcgc agccccgtgc ggcgcttata tacgattagg cgtaggttgc ggtaaaatga
      961 cggagcgtcc ttgctctttc cgggagccca gcggttttcc gatggggagt ggcacagcga
     1021 gcttgaaccg gcataggcgg gcggccacct ataaactgat tgatgccccg tgaaatgagc
     1081 ctggaatggt tagcgatcaa gacgttctcg ccgcgctgca aaaggttccg ggtcccgatg
     1141 gtaagactcc cctaacgagc tcgggtgtgc tttcaggtct gtcgattaag gacagcaaag
     1201 tttacgtgtc gatctccgtt gatccgcgcc actcgggagc gtttgagccg atgcgggcag
     1261 ccgccgaggc cgcgcttagg agattgccgg gcgttaccct ggcgctcgtt agcttgactg
     1321 ccgaaaagca ggcgccgcca aagccgatct cgtcgcgtgg cgttgcgatc ccggggattt
     1381 ccaacatcat cgccgtcgcc tcgggaaagg gcggcgtcgg caaatccacc tgctgcgtca
     1441 atctcgcgct cggccttgtg tccttgggtt ggcgcgtcgg cattctcgat gccgatgttt
     1501 tcgggccgtc gctcccgcgc ttgcttgggc tcaaggcaaa accagaatcc gaaggccgct
     1561 ccattcggcc gcttgttgcc tatggagtca aagccatgtc gatcggcttc ctagttgaag
     1621 aggaagatgc gatgatctgg cgcgggccca tggtgatgtc cgcgatccag cagttgctgc
     1681 gcgatgtttt gtggggcgag ctcgattgtt tgcttgttga tatgccgccg ggcacggggg
     1741 acgctcaact gacgctggcg caaaatgtac cgcttgccgg cgctattatt gtctcgacgc
     1801 cccaagatct ggccttgatc gacgcgaggc gcggcatagc catgttcaac aaggtcgcgg
     1861 tgccggtttt gggcatcatc gaaaatatga gctattttct atgcccgcat tgcggcgcgc
     1921 gctcggatat tttcgcccat ggcggcgcgc gcattgaggc cgaaaaatgc gccgttccgt
     1981 ttcttgggga agtgccgtta gatattggca tccgcgaaca ttcggattct gggcgtcctt
     2041 taactgcggt cgatcccgat ggccaaaatg ccaagatttt tcgcaccatc gccgcgaagg
     2101 taaaggcagc gcttgagacg ggccggacac gcgcggcgcc gaagattacg attgaatgat
     2161 actcgagccg tagcggtggg aagtaggggt cactggcggg tagtccgccg gactgcactg
     2221 gaagcagcta cttgcccggt tttcagtgga cagtgagggg cctctgtgta gctgctggcc
     2281 tgcaaccatc aagggtggag agcttttgga cccgcaccag gtgcctctcc ggtagggttg
     2341 atgatttcga actttccgga agaaaagccc attgctcgat tggtttcccc gggttccaat
     2401 cgttttggtt agggttcagt gcgattggat tcgatgtttc cgggaacagg caaagaatgc
     2461 tttgccctag tcaaaggctg catatattgg ggtttccaat cgttcgcagt ggtttttggc
     2521 aattgtttcc cgaaacgctt aagaaacttt gatgcacatt cggcgtggcc ttgcgggcta
     2581 gtcaggcgaa gctatgatga agctcggagc agcggctttt aggccgaggc gcgtgacttt
     2641 taaaggccgt tatcgggtcg gccggtttgg cgatgctgga cccggcgaga gggatcctgg
     2701 gcatgcacaa cgatggcaaa gctaaggccg agtctggcgc tgagatggag caggcgccag
     2761 ctgccaagcc agcgcgccaa ccgtctgccg aggctaagct tggagagctg aaaaggcgct
     2821 tacttgaaat cagcgatctt gaagccgtgg gttccgtcct cggctgggat cacgccacct
     2881 atatgccaac aggaggtgcc gccgcgagag cccgccaagg cgcaatgctg agcagcctca
     2941 ggcatgaaag atcagttgat ctgacgctcg gaaagctact cgataatctt ttaccccatg
     3001 ccgaaggttt gcgttacgat tcggatgaag caagcctcat tcgtgttgcc aggcgtgatt
     3061 ttgagaaggc aacaagactt cctgccgact atgtcgcccg ggcgagtgcc ctcagctcgg
     3121 cctcctacga tgcttggacc cgcgcaaggc ccgcaaacga cttcgcggcg atgcagcctt
     3181 ttctcgaaaa ggccgtggat cttgcccggg aatatgcaag ctattttgcg ccctatgaga
     3241 atgtcgccga cccgctgatc gattccgccg aggaaggcat gacggcggcc atgatcattg
     3301 agctgtttgc ccggctcaag cggcacttgc aaccaattgt aggagccatc tgcgatcagg
     3361 ctgttccctg tgatgactgt ctgcacggct ttttcagtga ggcggcgcag ctcgatttca
     3421 gcctttctgt ggtcaagcgc tttggctatg acacaaatcg cggaaggctc gacaagactc
     3481 atcatccttt ctgcaccaaa ttttccttag gcgatataag gatcacgacc cgcgtcaatg
     3541 aaaggcagtt tggaagcgcg ctatttgcgg ccatgcatga ggccggtcat gcgatttacg
     3601 agcagggtgt cgcccctgca cttgagggca cgccgctggg atcgggagcg tcatggggcg
     3661 tgcacgagag ccagtcacgg ctttgggaaa atttggttgg ccgcggccgt cctttctggg
     3721 aacattttta cccggttctg cgcaatgagt ttcccgatca gctcagggga atagacgacg
     3781 agaccttcta ccgcgcaatt aacaaagtgc aacgatcttt gattcgcacc gatgccgatg
     3841 aggtcaccta caaccttcat attatgatgc gcttcgactt ggagttggaa ttgctcgagg
     3901 gtcgtctgcg cgtaaaagat ctccccgaag catggcgcgc gcggatgaag accgatctcg
     3961 gtatagctcc tgacgatgat cgcgatgggt gtctccaaga cgtgcactgg tatggcgtcg
     4021 gcacagtcgg cggcggattt caaagctata caattggcaa cattctcagc gcgcagttct
     4081 atgatgctgc aatgaaagct catcctgaga ttccaaatga gatagcaaat ggcgagtttg
     4141 gcaccgtgca cggatggctg cgcactcatc atctaccaac atgggcgcaa gtttgggccc
     4201 ggcgagctcg tctttcgtgc ggccggagag cctatgagca tcgcacccta catcgaatat
     4261 cttcaccgga agtacggtga gctttatcgc ttgccggcca ttggcaaaat tgatcagcca
     4321 gaggtgaaag cgcaagcgca ctcttagtgc cgcagaggca gaaattggcg ttcaagagcc
     4381 gcctgttaga tgtgatgctt gcgagtttga ccgtggaggg tgagcgggtg ccgcggctcg
     4441 ctagtcgttt ggtttgatgc cagaaacaaa cacctgttgc cgcgagcaga aagaactacc
     4501 catcgctgaa ataggacatg tggttgacta cgaagagtga acttgtcgcg aaggagatct
     4561 gctgcttgat cctcatcatc cgagcggctt caacggcggt tccatcaacc atgatttcat
     4621 ggttttgccc ggctgcctga caaa
//
LOCUS       DATMPU010000224         3865 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_1111727,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000224 DATMPU010000000
VERSION     DATMPU010000224.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 3865)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3865)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3865
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1111727"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            588..2087
                     /locus_tag="VNY06_05145"
     CDS             588..2087
                     /locus_tag="VNY06_05145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012592477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjX family protein"
                     /protein_id="HWX14229.1"
                     /translation="MPLISDLVFEARAAVKKLVELSTGPHIRLGVTGLSRAGKTIFIT
                     ALIEHLTKAASPALRGRKNTLPVFRVHADGRLIGGTLEPQPDDAVPRFAYEDHIAALS
                     GPKDSSGSRHWPESTRRISELRLRLYFDRPAGMQPSRRSLTIDIVDYPGEWLLDLPLL
                     TKSYAQWSRETFIASASAARARLAANWRGLAAITNARGEASEEQARREAEVFTAYLRA
                     CRAGEYHFSTLPPGRFLMPGDLEGSPALTFAPLAVEEGEIYPPESYAAMMERRYEAYK
                     TYVVKPFFRDHFSRLDRQIVLIDTLAALNAGPAAMRDLETALTDVLGVFRAGRSNILT
                     TLFRPKIDKILFAATKADHLHHTSHDRLEAILRFLTRRAIARAENVGAEVDVIALAAV
                     RATREAKVGGPNEPALEAVVGTPLDGEQIGDEIFDGIAEAAIYPGELPSDPEAVFREM
                     PRAKPDEIDYRFVRFLPPAPVRASDGTLLPPPHIRLDRALQYLFGDRLG"
     gene            2181..3233
                     /locus_tag="VNY06_05150"
     CDS             2181..3233
                     /locus_tag="VNY06_05150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012592476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01620 family protein"
                     /protein_id="HWX14230.1"
                     /translation="MAIEPKPRPRAFRIGGTANARVGAPPPPTVIESKVDPYEAEAMA
                     LIAAQDRGAVSVKAQKKRSFAKSSLLSWAGLFWSATGGLVSLAFGLWLARLIEDLFSW
                     YPAFGVVGLALAGLAGTALLVLGVREVSGFLRQRHISDLHAELGLAREKNDFDGARRL
                     TRQLSELYSGKPEAVHARNQLRELSHDIVDGRDLIDIAERALVAPLDAEVRREIARAA
                     KRVSMVTALAPRAIIDVIFVAAQAIRLIRRVAVIYGGRPGFLGLLKLLRSIAAHIAVT
                     GGMAAGDSLIQQFLGHGIAAKLSVRLGEGVLNGMLTTRVGLSAMAVCRPLPFATGKAP
                     KVADVAPFLFRSEKKE"
     gene            3640..>3865
                     /locus_tag="VNY06_05155"
     CDS             3640..>3865
                     /locus_tag="VNY06_05155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020175150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF302 domain-containing protein"
                     /protein_id="HWX14231.1"
                     /translation="MIFGIWQYGVYAMAADGLITIPSSFGPKETMDRLEVEIKAKGMT
                     VFARIDHAAGAEEVDLPLRPTELLIFGNAKA"
BASE COUNT          754 a         1129 c         1103 g          879 t
ORIGIN      
        1 taagcgcctt ctcgagaata tggccaacgt cgaaatcggg ccactcaagg tgcggactga
       61 gctcatagct gcagcgatag agaggctgac tactaaaact ccggcggagg gatcgctagc
      121 caattctccc ggcatcacaa ctaacgagac cgcgcgctcc aagcagtgag ttccgtcccc
      181 tagagcaaga tgacttttct tcgaatcgtc ctcttgctct agttctttgt tttgtcgcgt
      241 gtccggacgc aaaaccggtt tccacttttg ctgggcacgc tctagagcat gataactttt
      301 ctttaaatcg tcatcttgtc tcatcctttg ccttgtcgct ttcgagacga aaattggaag
      361 cacttcgtca gcgtggattc cacttgaatc tagcgcagtg ccattaattt agcacaatgc
      421 ccttcgatgg tctttcctcc atcgggccgc cctgaccgag cgcaacggct ctggcccgtg
      481 tcaagcctta ccggtcaagc gcgttgccta ttcgtagagt tgacctactt aatcgatagg
      541 gacacggtcc aaagcccaag taaagctagg tagctggaat ctttctcttg ccgctcatct
      601 ccgaccttgt ttttgaagcc cgcgcggcag tgaaaaagct tgttgaactg tcaaccggcc
      661 cgcatatccg cctcggcgtg acgggacttt cgcgagcggg aaaaacgatc ttcattaccg
      721 ctctcattga acatctgacc aaagcagcaa gccccgcttt acgtggccgc aagaatacgt
      781 tgccggtctt ccgggttcac gccgacgggc ggctcattgg gggcacgctg gagccgcagc
      841 ccgacgatgc tgtgccccgc tttgcctacg aggatcacat agccgcgctt tcgggcccca
      901 aagattcctc cggctcccgc cactggccgg aatcaacgcg gcgcatctcc gagctgcggc
      961 tgcgtcttta tttcgatcgg ccagccggca tgcagccatc gcgtcgatcg ctcaccatcg
     1021 acatcgtcga ttatcccggc gaatggctgc tcgatctccc tctccttacg aaatcctatg
     1081 cgcaatggtc gcgcgaaacg tttattgcct cggcaagtgc cgcacgagca cggcttgccg
     1141 caaattggcg agggcttgcg gccattacca atgcccgcgg tgaagcgagc gaggagcaag
     1201 cgcgccggga ggctgaagtt tttaccgcct acctgcgtgc ctgccgcgct ggagaatatc
     1261 acttttctac cttgccgcct ggacgttttc ttatgccggg ggaccttgaa ggctcccccg
     1321 ccctcacctt cgcaccgctc gctgtcgaag aaggtgaaat ctatccgccc gagtcttacg
     1381 cggctatgat ggaacggcgc tacgaggcct ataagactta tgtcgtgaaa ccctttttcc
     1441 gcgaccattt ttcaaggctc gaccgccaga tcgtcctcat cgataccctc gccgcattga
     1501 atgcaggccc tgccgcaatg cgcgatctcg agaccgcgct caccgatgtt cttggggtgt
     1561 ttcgagccgg gcgtagcaat attttgacga ccttgttccg gccgaagatt gacaagattc
     1621 tttttgccgc aacaaaggcc gatcatctcc accacacgag ccatgaccgg ctcgaggcca
     1681 ttttgcgctt cctcaccaga agagccattg cgcgggccga gaatgtcggc gcggaggtgg
     1741 atgtgatcgc gctggctgca gttagggcaa cgcgcgaagc caaggttggc ggcccgaatg
     1801 agccagctct cgaagccgtc gtcggcacgc cgcttgacgg cgagcaaatt ggcgatgaaa
     1861 tttttgatgg aatcgcggag gcggcaattt atccaggcga actcccatct gatccagaag
     1921 cggtgtttcg cgaaatgccg cgcgcaaaac cagatgaaat cgactaccgt ttcgttcgct
     1981 tccttccccc cgcccctgtt cgcgcaagtg atggcacgct gctgccgcca ccccatatta
     2041 ggctcgaccg cgcgctccaa tatctcttcg gtgacaggct cggctaatgg cctcgaccgt
     2101 ctttgatgct tgattgtctg ttttctgtcg tgattcgaag cgggcaagag gcgcctggca
     2161 aaggagcaac gaggtctagc gtggcaattg aaccaaaacc ccgtccgcgc gcgttccgta
     2221 ttggcgggac cgccaacgcg cgcgttggcg cgccaccgcc tccgacggtg atcgaaagca
     2281 aggtcgaccc ctacgaggcc gaagccatgg ctttgatcgc cgcccaagat cgtggcgccg
     2341 tgtcggtcaa agcgcagaaa aaaagaagct ttgccaagag ctcgcttcta tcctgggccg
     2401 gtctcttctg gtccgctacc ggcggccttg tttcgcttgc cttcgggctt tggctcgcgc
     2461 ggctgatcga ggaccttttc tcctggtacc cggcctttgg cgtcgttggt cttgcgcttg
     2521 cgggcttggc cggaaccgca ctgctcgttc tgggcgtacg cgaagtcagc ggcttcttgc
     2581 gccagcgcca tatctccgat ctccatgcgg agcttggcct tgcgcgggaa aaaaatgatt
     2641 ttgacggagc gcggcggctc acccgccagc tttctgaact ttactccggc aagcccgagg
     2701 ccgtgcatgc gcgcaatcaa ttgcgtgaac tttctcatga catcgtcgat ggccgcgatc
     2761 tcatcgacat cgccgagcgc gcgctcgtcg caccgcttga tgcggaagtg aggcgcgaaa
     2821 tcgcgcgcgc ggcaaagcgc gtttctatgg tgacagcgct cgcgccgcgc gcgatcatcg
     2881 atgtgatttt tgtcgcggcg caggcgataa ggctcattag gcgtgtcgcg gtcatctatg
     2941 gcggcaggcc gggattcttg gggctattaa aactcctgcg ttccattgct gcgcatatcg
     3001 cggtcacggg cggcatggcg gcgggcgaca gtcttattca gcaatttctt ggccatggga
     3061 tcgccgccaa actttcggtg cgtcttggcg agggcgtatt gaacgggatg ttgacgacgc
     3121 gcgtcgggct gtccgccatg gcggtctgca ggcctttacc ctttgcgacc ggcaaggcgc
     3181 caaaagttgc cgacgtcgcg ccctttctat ttaggagtga aaagaaagag tgacgcggcg
     3241 aagtgcgagc gttttgtccg atttcgagcc aaaaccttac caaaatgttt cacgtgaaac
     3301 atttttgtcc gatcaggccc caaaacctta caaggcctct tacgtctggt ggtttgaaag
     3361 agtaggattg cgcagaattt tggtacattc ggcgggtgct ccgcgaggtc attttggacg
     3421 ggcttttggc cacacgcgtc gggctttcgg cgatgtcggt agtcgccttc tgcgttaagc
     3481 gaccgcgcga gcgataaacc tttttcggtc attggcgtgc cggttgtgat gaaactctcg
     3541 gatagcttca gactggataa gtgccttccg acgacattcg aaatggcata ttgactgaaa
     3601 gacagaacag gcctcttggc ggcgagcaga ttcgtcgcga tgatcttcgg gatctggcaa
     3661 tatggtgtgt acgcgatggc ggcggacggg ttaatcacca taccgagtag ctttgggccg
     3721 aaggaaacga tggatcggct cgaagtcgag attaaggcca agggcatgac ggtgtttgcg
     3781 cgcatcgatc atgcggcggg agccgaagag gtcgatttgc cgctgcgacc aaccgagctc
     3841 ctaatattcg gcaacgccaa agccg
//
LOCUS       DATMPU010000225         4058 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_4505586,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000225 DATMPU010000000
VERSION     DATMPU010000225.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 4058)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4058)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4058
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4505586"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(13..3273)
                     /gene="treS"
                     /locus_tag="VNY06_05160"
     CDS             complement(13..3273)
                     /gene="treS"
                     /locus_tag="VNY06_05160"
                     /EC_number="5.4.99.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020173819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047471 - maltose
                     alpha-D-glucosyltransferase activity [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose alpha-D-glucosyltransferase"
                     /protein_id="HWX14232.1"
                     /translation="MINRDDREWYRDAIIYQLHVKSFFDANNDGIGDLAGLTEKLDYI
                     RDLGATAIWLMPFYPSPQRDDGYDISYYRGINRAYGSLRDFKRFVREAHERGLRIITE
                     LVINHTSDQHPWFKRARAAKPGSSARSFYVWSDTDHAYKDAPVIFLDTEKSNWTWDEQ
                     AKAFYWHRFYAHQPDLNFDNPRVLQAVLGVMDYWLDMGVDGLRLDAVAYLVEREGTNC
                     ENLAETHALIKRIRAAVDAKYPDRMLLAEANLWPEETAQYFGDGDECHMAFHFPLMPR
                     IYMALAQEDRHPITDIMRQTPEIPSGAQWAIFLRNHDEMTLAMVTSKERDYLWSFYAA
                     DPRARINLGIRRRLAPLLENDRRKIELLTSLLLSMPGTPILYYGDEIGMGDNIYLGDR
                     DGVRTPMQWSLDRNGGFSRADPAKLFLPAIQDSIYGFYAVNVEAQLASSSSLLTWMRR
                     MIAVRRSHKSFGRGTLRFLHPSNRKVLPYIREFEGERILCVANVSRAPQAVELDLSDL
                     KGCVPVELTAGSYFPVIDSRSYLLTLPAYGFFWFRMEEAEASKERQEQQPAPGLYTLV
                     ATGKLETILVGRERIAFERTVAPRFLSSRYWFGHEGKPIVVVSVKDFAVLRDGADCRL
                     VLPILDVEFRGGEVETYFTPLAAEVEREEGPSLVHAAARLRRGMMTGLLYEAEAGEHF
                     ARAMLAAFQRGDALVTGDGGRISFLPSPSLAAEPLTEDANVRRIGLGRRNSSLVLANQ
                     IMLKIHRRLEVGEDPEVEVLRFLTETAHFANCPALLGVVEYVDRAENRTVLAILQTYV
                     RNQGDARAWMLDALKRSLEAAALTPGQDDRAGHEEFAAFVPHLRRLGFYTAGMHMALA
                     TPCADPAFKAEELSLDDIRVAAEATRILAGRAFARLRTIAVSFSGDKRAGAERLLARQ
                     QECLCLIGKLIQKPHGAIKIRIHGNYRLRRLLVVKDDIVIVDFGGETSVPIDQMRAKT
                     SPLRDVASMLRSFVHAVAAAKQDLARLVPDPDHAAARLRDELIEFSQIFIRAYWEAAR
                     DSPIWIEDEGTRRRLLVLYLLNELLHDIESDSETRPEWIDMSVESVNTILDRMANE"
BASE COUNT          929 a         1159 c         1136 g          834 t
ORIGIN      
        1 cccccgcggg catcattcat tagccatccg atcgagaatg gtgttgacgc tttctaccga
       61 catgtcgatc cactccgggc gcgtttccga gtcggattca atgtcatgca gcagctcgtt
      121 gagcagatac agtacgagta gtcgcctgcg cgtgccctca tcctcgatcc agatggggct
      181 gtcgcgcgcg gcctcccaat atgcccggat aaaaatttga gaaaactcaa ttagctcatc
      241 gcgaagcctg gccgctgcat ggtccggatc cggaacgagc cgcgcgagat cctgcttcgc
      301 tgcggcaacg gcatgcacaa aggaacgcag catggaagcg acatcccgca gaggcgatgt
      361 cttggcgcgc atttgatcga tgggtaccga agtctctcct ccaaaatcaa caatcacgat
      421 atcgtccttc acgaccagaa ggcggcgaag acgataatta ccatgaatac ggatcttgat
      481 cgcaccatgt ggcttctgga taagcttgcc aattaggcac aaacactcct gctgccgggc
      541 caaaaggcgt tcggccccgg cacgtttgtc gccactgaag ctcacggcaa tcgtcctcag
      601 gcgggcgaag gcgcgcccgg cgagaattct tgtcgcctcg gctgccacgc ggatatcatc
      661 gagcgacagt tcctcggctt taaacgcggg atcagcgcaa ggtgtcgcga gcgccatgtg
      721 catcccggct gtataaaagc cgagtctgcg cagatgaggc acgaaggcag caaattcctc
      781 atggcctgcg cggtcgtctt gcccaggggt caaggcagcc gcctccaggc tgcgtttcaa
      841 tgcgtcgagc atccaagccc gggcgtcgcc ctggttgcga acataggttt gcaggatcgc
      901 aagcaccgta cggttttccg cgcgatcgac atattcgaca acgcccagca aggccgggca
      961 gttagcgaaa tgagccgttt ccgtcaaaaa gcgtaggact tcgacctcgg gatcttcgcc
     1021 gacctctaac cgacggtgaa tcttcagcat aatttgattc gcaagaacaa gtgaggagtt
     1081 gcgccgtcct agcccgatcc ggcggacatt ggcatcctcg gtcaatggct cggcagcgag
     1141 gctgggcgac ggcaagaatg aaattctccc accatcgcca gtcaccaacg catcgccgcg
     1201 ctggaacgct gccagcattg ctcgggcaaa atgttcgccc gcctctgcct cataaagcag
     1261 gccagtcatc ataccacgcc gcaatcgcgc ggcggcgtgc acaagcgacg gcccttcttc
     1321 ccgttcaact tccgcggcga gcggcgtgaa ataagtttca acttcgccgc ctcggaattc
     1381 aacatcgagg atcgggagaa cgagacggca gtccgcacca tcgcgcagca ccgcgaagtc
     1441 tttgaccgaa accacgacga tcggcttccc ttcgtgccca aaccagtacc gcgaggacag
     1501 gaagcgcggc gcgaccgtgc gctcgaaggc gatacgttcg cgccccacga ggatcgtttc
     1561 gagcttgccg gtggcaacca gtgtgtagag tcccggtgcc ggttgctgct cctggcgctc
     1621 cttgctggcc tccgcttctt ccattctaaa ccaaaagaaa ccgtaggccg ggagggtcaa
     1681 tagataggac cgcgagtcaa ttaccggaaa gtagctgccg gcggtaagtt cgacaggcac
     1741 acaacccttt aaatcactga ggtcaagttc gaccgcctgg ggtgctcgcg agacatttgc
     1801 tacgcaaaga atccgctcgc cttcgaattc gcggatgtag ggcagcacct tgcggttgga
     1861 agggtggaga aaccgcaacg tgccgcgccc aaatgactta tgcgaacggc ggaccgcaat
     1921 cattcgtcgc atccacgtca atagactaga tgagcttgcg agctgggctt cgacattgac
     1981 ggcgtaaaat ccatagatcg agtcttggat tgcgggcagg aagagcttgg ctggatcggc
     2041 acggctgaag ccgccgttgc ggtcgaggga ccattgcatt ggcgtccgta caccgtccct
     2101 gtcgccaaga tagatattgt cgcccatgcc aatctcgtcc ccgtaataaa gaatcggggt
     2161 gccgggcatc gataacagca gcgaagtcaa aagctcgatt ttgcgacgat cgttttcgag
     2221 caagggcgcg agccggcggc ggatgcctag attgatccgc gcacgagggt cggctgcata
     2281 gaaagaccat agatagtcgc gctccttgct tgttaccatg gcaagagtca tctcgtcatg
     2341 gttgcgcaga aagattgccc attgcgcgcc ggacgggatt tcgggggtct ggcgcatgat
     2401 gtcggttatc gggtggcggt cctcctgcgc caaagccatg tagatgcgtg gcataagcgg
     2461 aaaatgaaat gccatgtggc attcatcgcc gtctccgaaa tattgggctg tctcctctgg
     2521 ccaaagattg gcttcggcaa ggagcatccg gtccggatat tttgcgtcga cggcggccct
     2581 gatcctcttg atgagcgcgt gtgtctcggc cagattttca caattggtcc cttcacgctc
     2641 aacaagataa gcaactgcat cgagccgcaa tccgtcaaca cccatgtcga gccaatagtc
     2701 catcacgcca aggacggcct gcaggacgcg aggattatcg aaattcaagt ctggctgatg
     2761 ggcataaaag cgatgccaat aaaatgcttt tgcctgctca tcccaggtcc aattcgactt
     2821 ttcggtatca agaaagataa ctggcgcgtc cttgtaagcg tgatcggtat ccgaccaaac
     2881 ataaaagctc cttgccgacg agccgggctt cgccgcgcgc gcacgcttaa accagggatg
     2941 ctgatccgac gtatggttga tcaccagttc cgtgatgatg cgaagtccac gttcatgcgc
     3001 ctcgcgcacg aaacgtttga aatcgcgcag gctcccatag gcacggttga tgccgcggta
     3061 gtaggaaatg tcatagccgt cgtcgcgctg aggagacgga taaaagggca tcagccaaat
     3121 cgcggtggcg ccgaggtccc tgatgtaatc cagcttctca gtgaggccgg ccaaatcgcc
     3181 gataccgtcg ttgttggcat caaagaagga tttcacatgc aactggtaaa taattgcgtc
     3241 gcgataccat tcccgatcgt cgcgattaat catacagtgc tccggcacgt gtctactggc
     3301 ggccttagct tacagttgcc aaaaaaatag cccgaccgcg cagcctgtaa tttccgatga
     3361 acgcagcctt ggaccggaac aaagctaccg gttgcgcttg ccggatctaa ccctagagcg
     3421 tgtccagcaa agtggaaacc ggttttaaat cccgacacgc gacaaaacaa gaatctagag
     3481 caagatgacg attcgaagaa aagtcatctg gctttagccg cggtgtcaat cctgatcgtt
     3541 gcgttgtttc cttaagtttg cccaatattc gcatccagcc tctagcggca gaacaatgaa
     3601 cctgtctgat tgatagcatg cctaccacaa atccgatggc gcgctagccg caaggcagcg
     3661 aacgtctgca gaaatggaaa aggcgaaagc tcctgctggt ccaggccgcg ggtttcgatc
     3721 tcgcagtact ggcgtaagct agcaaggcaa attgagccct tagaacgccg gtggaaagac
     3781 gcggctgcaa ggcggtcggt gagcttctgc agaaaatggc ccccgccggt tcatgctggc
     3841 tgacgctcga cgcgagcagc aagcaggtct ttcatgtcct gctccaacct cgtgatgatc
     3901 ttatcatggc tttcaatcat ctgattccgt gccaacgtgt caggggaaaa ccccggccgt
     3961 ggccaagaag cgcaacgtac tggcgttccg cttttaaagc cgcggccgtg tgttggatga
     4021 attgttcaat ttcggcatcc gtcagctggt cacattgc
//
LOCUS       DATMPU010000226         3151 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_2052035,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000226 DATMPU010000000
VERSION     DATMPU010000226.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 3151)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3151)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3151
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2052035"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..560
                     /locus_tag="VNY06_05165"
     CDS             <1..560
                     /locus_tag="VNY06_05165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018407444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HWX14233.1"
                     /translation="NVPLPHEPRPVFRASSTARLLVASQAPGVRVHRTGLPFNDASGD
                     RLRQWMNVSRETFYDEARIAIVPMGFCFPGHDFNKADLPPRRECREAWHDLLFSKLPQ
                     IECILAIGRFAQDYHFARAGRQLPKGLSVDERVRRWQEFSGEAPRIIALPHPSWRNSG
                     WIKRNPWFESEVLLALRAEVARAIT"
     gene            853..1920
                     /locus_tag="VNY06_05170"
     CDS             853..1920
                     /locus_tag="VNY06_05170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018266781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ppx/GppA phosphatase family protein"
                     /protein_id="HWX14234.1"
                     /translation="MRGAPAGDPDGVLAGWQADEHIYAALDLGTNNCRLLIARPAPAG
                     RGEDGFRVIDSFSRIVRLGEGLSKTGELSAAAVERTLGALEICKDKMRARGVTRSRLI
                     ATEACRSAINGAEFLNRVRKNVGLELEVVDRETEAQLAAMGSASLVDSAAEGVVLFDI
                     GGGSSELVWLARAAEEAGQWAEAALRNRIRFWVSLKLGVVTLAEQFGGIEVSDTTFEE
                     MINRALREIEDFVVMAGGERRCGLFHLLGTSGTVTTVAGVYLGLSRYDRRRVDGLWMS
                     SGQVDHAVQRLRNMSYMERAANGCIGEGRADLVLAGCAILEAIRRAFPADRIRIADRG
                     LRDGILIQLMRADRGVLPGKT"
     gene            1920..2624
                     /locus_tag="VNY06_05175"
     CDS             1920..2624
                     /locus_tag="VNY06_05175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012384907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RlmE family RNA methyltransferase"
                     /protein_id="HWX14235.1"
                     /translation="METRASSGREGGRSLKNRVKTARKRSLSSTLWLERQLNDPYVER
                     AKREGFRSRAAYKLLEINERYHFLKPGQRIVDLGAAPGGWSQLCARITGAEEGRGRII
                     GIDLIEITPLPGAEFRVLDFMTPEAPQLLKDWLSGNADVVLSDMAANATGHKKTDHLR
                     IMGLAELAADFACEVLTEGGTFLAKVFQGGAEIELLARLKSEFANVRHVKPQASRAGS
                     AELYVLATGFRGGSEG"
     gene            complement(2637..>3151)
                     /locus_tag="VNY06_05180"
     CDS             complement(2637..>3151)
                     /locus_tag="VNY06_05180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012590364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWX14236.1"
                     /translation="AGAMAMKATAAPILRRFGFRPVLIYNAVLSAAFLGCYGFFTPST
                     PVALMMSLLFVGGFLRSLEFTSLNAIAFAEIDALQMSRAVSLSSVAQQLSLSLGVAAG
                     AGALQGFALLNPGTGVLALDNFRWAFVAMASVSLTAVAAFLRLPAEASAELTQRGTQS
                     STKAISPAGQ"
BASE COUNT          633 a          857 c         1022 g          639 t
ORIGIN      
        1 gcaacgttcc gctcccgcat gaaccgcgtc ccgtctttcg cgcatcttca acggcgcggc
       61 ttctagtggc aagtcaggcg cctggcgtga gggttcatag gactgggctg ccatttaacg
      121 atgcctctgg agatcgtctg cggcaatgga tgaatgtttc gcgcgaaaca ttctacgatg
      181 aggcaaggat cgcgattgtg ccgatgggat tctgctttcc aggccatgat ttcaataagg
      241 ccgacctgcc gccgcgacgc gaatgccggg aggcttggca tgatctcttg ttttcgaagc
      301 tgccgcaaat cgaatgcatt ctagcgatcg gccgctttgc gcaggactat catttcgcgc
      361 gtgcagggag gcaattgccg aaaggtctga gcgtcgacga gagagttcgc cgatggcagg
      421 aattttcggg cgaagcgcca agaataatcg cccttccaca tccctcgtgg cgcaacagcg
      481 gctggatcaa gcgcaatcct tggtttgaaa gtgaggtcct gttagcgtta cgtgccgaag
      541 ttgcgcgcgc aatcacctga gagcacctaa gctgggcagg ggcgggttct cggtgcgaac
      601 gccgcccttc gccccccttt tttctccgga ggagcggtcg caatacaggg cgctttcagc
      661 gtttggtgtt tcagcttcgg cttgctacac tttgtcgcga atggaattcg attgggtccg
      721 caggctcagg ggggcaaggt gaaaccagtg gacgcggccg gacccgcagg ccttcaaggt
      781 cacgtccttc gaggaaaaac tctgcctaag gctgcgggcg ccgttcccgg cgcgaatacg
      841 acgagacgag gcatgcgggg ggccccagct ggcgatccgg atggggtttt ggctgggtgg
      901 caagcggatg aacacatcta tgcggcgctt gacctcggca ccaacaactg ccggcttttg
      961 atcgccaggc cagcgccggc aggcaggggc gaggatggtt ttagggtcat tgactcattt
     1021 tcccggatcg taaggttggg cgaaggcctg tcgaaaacgg gggaacttag cgcggcggcg
     1081 gtcgaacgaa cccttggcgc gcttgaaatt tgcaaggata agatgcgcgc gcgtggcgta
     1141 acgcgcagcc gtttgattgc gactgaggca tgccggtctg cgattaatgg cgccgaattt
     1201 ctcaatcgtg tgcgaaagaa tgtagggctc gaactcgagg tcgtcgacag agaaacggaa
     1261 gcccagctcg ctgccatggg ctcggcgtct ctcgttgatt ccgccgcgga aggcgtcgtg
     1321 cttttcgata tcggcggtgg atcctctgaa cttgtctggc tcgcgcgtgc tgctgaggag
     1381 gccgggcaat gggcggaggc ggcacttcgc aatcgcattc ggttctgggt ctcgcttaag
     1441 cttggcgtcg tcaccttggc tgaacagttt ggcgggatag aggtttccga taccaccttc
     1501 gaggagatga tcaatcgcgc tctgcgcgaa atcgaggact ttgtcgtcat ggccgggggc
     1561 gagcggcgct gcggcctctt tcatttgctg ggcacttcgg gaacagtgac gaccgttgcc
     1621 ggtgtgtatc tggggctctc ccgctatgac cggcgccgcg tcgatggttt atggatgtcg
     1681 agcgggcaag tggatcatgc ggttcagcga ctgcggaata tgagctatat ggagcgcgcg
     1741 gccaacggct gtatcggaga gggacgtgcc gatctcgttc tcgccggctg tgcgattctt
     1801 gaagcaatcc ggcgggcgtt tcccgctgac cgcatccgaa tcgccgaccg gggattgcgc
     1861 gacggaattc tgatccagct catgcgcgct gatcgcggtg tgctgccggg aaaaacctga
     1921 tggaaacccg cgcttcttcc gggcgcgaag gcggtcgctc gcttaaaaac cgcgtcaaga
     1981 cggcgcgcaa gcgctccctt tcgtcgaccc tgtggctcga gcgccagctc aacgatcctt
     2041 atgtggaaag ggcgaagcgt gagggtttcc ggtcccgtgc ggcctataag ctgcttgaga
     2101 tcaatgagcg ctatcatttc cttaaaccag gccagaggat tgtcgatctc ggggcagccc
     2161 cgggaggttg gtcgcagctt tgcgcgcgaa ttaccggggc agaagagggc agggggcgga
     2221 tcatcggcat tgatctcatc gaaatcacgc cgctgccggg tgctgagttc cgcgttctgg
     2281 atttcatgac cccggaagcg ccgcagttgt taaaggactg gctttcgggc aatgcggacg
     2341 tggtgctgtc cgatatggcg gcgaatgcca caggccacaa aaagaccgat catttgcgca
     2401 tcatgggcct cgccgagctc gcggccgatt ttgcttgcga agtattgacc gaaggcggaa
     2461 ctttcctcgc caaggttttt caaggtggcg cggaaataga gctcctcgcc cggttgaaaa
     2521 gcgaattcgc gaatgtgcgc cacgtaaaac cgcaggcaag ccgcgccggt tcggcagagc
     2581 tttatgtgct ggcaacaggc tttagaggcg gaagcgaggg ataagcgaag gcgctatcat
     2641 tgccccgcgg gagaaattgc tttggtggaa ctctgcgtgc cgcgttgcgt aagttcagcg
     2701 ctggcctccg cgggcaggcg caggaatgcg gcaaccgcgg ttaacgagac agaagccatc
     2761 gccacaaatg cccatctaaa attgtcgagg gcgaggacgc ccgtgccagg gttgagaagc
     2821 gcaaagcctt gcaaagcgcc tgctccagca gcgactccaa ggctcaagga caattgctgg
     2881 gcaaccgaag acaaactgac ggcccggctc atttgcaacg catcgatttc ggcaaaggcg
     2941 atcgcattga ggctcgtgaa ttcgagcgaa cgcaaaaagc cgccaacaaa aagcaggctc
     3001 atcatgagcg caaccggcgt ggagggtgta aagaacccat agcagccgag aaacgcggcg
     3061 ctcagcacgg cattatagat gagcaccggg cgaaagccga aacggcgcaa gataggcgct
     3121 gccgtcgctt tcattgccat cgctcccgcc g
//
LOCUS       DATMPU010000227         3023 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_805406,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000227 DATMPU010000000
VERSION     DATMPU010000227.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 3023)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3023)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3023
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_805406"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..382)
                     /locus_tag="VNY06_05185"
     CDS             complement(<1..382)
                     /locus_tag="VNY06_05185"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020177163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HWX14237.1"
                     /translation="MKRWDLSKVSRHPSQTAGLRLRVRTDADCASLADLWVASWREAM
                     PDTDFPARRPWFLDHLRALEAAGAITICALDGSNRLLGFVTFDPATAYLDQLAVAPEV
                     KGTGAAKLLVAEARRLSPNGLVLDV"
     gene            410..535
                     /locus_tag="VNY06_05190"
     CDS             410..535
                     /locus_tag="VNY06_05190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX14238.1"
                     /translation="MDPRDFRGIGGEAQEALHEPAVYPLMHEGMTQGKAAQGKRD"
     gene            734..1114
                     /locus_tag="VNY06_05195"
     CDS             734..1114
                     /locus_tag="VNY06_05195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX14239.1"
                     /translation="MMKKLLFSVLFASMGFGANTAMADGWPLSVLGNWSVLANQSAGT
                     LSITSQGSIGNCRPITGTIFGNTMVGFYCPFSGRIHFLRNSGATTIQDYTANVSQAGP
                     VLHMGGTFASDLGSFGEYNFSGTK"
     gene            1433..>3023
                     /locus_tag="VNY06_05200"
     CDS             1433..>3023
                     /locus_tag="VNY06_05200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012589950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HWX14240.1"
                     /translation="MSSVAVEDFAKYLADKHFLLSDTEFHSASSSASNAERSLRKLWE
                     STELTANGFADEVAQFYGLKRLTLPQLVAAPSLTGRFSPRFLRDTAVFPFELPKGQYK
                     LAVADPSDAAAVRAAEIVLGTPVLVEIASFEDIATVLGEQLGGEETTAGDGDALGARA
                     EDDIESLRDLASGAPVVRAVNDLLEKALELRASDIHIEPFRNGLVVRMRVDGLLRAVA
                     APANALPQALISRIKILAGLNIAERRLPQDGAARQKVARSEIDIRVATMPSQHGESAV
                     IRLLPRDRGLLDVAKLGLSIADEKIVRRLLALPHGMIILTGPTGSGKTTTLATMLSIL
                     NDPSRKILTIEDPVEYEIQGVNQSQVKPAIGLTFATAMRAFVRQDPDVIMVGEVRDAE
                     TANIAVHAALTGHLVLTTLHTENAAAAVPRLLDLGVEGFLLKSTLRAVIAQRLVRILC
                     DRCKRKRILTSGDLAADPRFDALGLRSGDCVYEPAGCERCGGAGYRGRNGVFEALELK
                     DEVYELVGPHADARAIDLAARH"
BASE COUNT          622 a          880 c          913 g          608 t
ORIGIN      
        1 tgacatcgag aacgagaccg ttcggcgaaa gccggcgtgc ctcggcaacg aggagctttg
       61 ccgcgccggt tcctttcacc tcgggcgcaa ccgcgagctg atcgagatag gccgttgccg
      121 gatcgaaggt cacgaagccg aggagccggt ttgaaccgtc aagagcacag atcgtgatgg
      181 ctccggccgc ctccagcgcc cgcaaatgat cgagaaacca tggccgccgc gccggaaaat
      241 ccgtgtccgg catggcctcc cgccagctcg ccacccaaag atcggcaagg ctcgcgcaat
      301 cggcatcggt tcgaacccgt aggcgcaatc ccgcggtttg tgaaggatgc cgcgacactt
      361 tgctaagatc ccaacgtttc ataatgactg aggggtatca tgaaggtgca tggacccgag
      421 agattttcgc ggcattggcg gggaggcgca agaagcgctg cacgagcccg cggtgtatcc
      481 cctgatgcat gaaggcatga cccaaggcaa agccgcgcag ggtaaacgcg attagcgaaa
      541 tcgtcatccc ctcaacaggg ggtgcggagg aacagcgatt atgctcaatt tgcgggtgcc
      601 ggacacgttg gacatggtcc gcttgccagg caatgacaag cttttccgac aaatcgggtt
      661 tgagcacaaa cctgctttgc cgggaggatg tccttaactt tagacggccg atttaactct
      721 caaaaaggag attatgatga aaaagctgtt atttagcgtg ttattcgcgt caatggggtt
      781 cggagccaat accgccatgg cggacgggtg gccgttgagt gtgctgggaa actggtccgt
      841 gttagcaaat cagtctgccg gcacgctcag cattacctct caaggttcca ttggcaactg
      901 tagaccgatc acggggacga tttttggcaa taccatggtg ggtttttact gcccattcag
      961 tggccgcatc cacttcctgc gcaattctgg cgcaactaca atccaggatt acaccgcaaa
     1021 tgtatcgcag gcgggaccgg tacttcacat gggagggaca ttcgcatctg atctgggttc
     1081 atttggggaa tacaatttct caggtacgaa ataataaccc gttctcattt gcctctgtga
     1141 accgcgcgat cgatccaaca ataggaccgg tcgcgccggt tcgcagtcaa ttcaagggcg
     1201 ctgtcaactg gtggttgaga agtagagaat ttaagactgt ccccgcagcg ccagacggcg
     1261 gtcgtgaccg tcgcgggggg aatgggtcga aatggctgga aaagagcccg ccaatgtcgt
     1321 atcctcggcc atggcaaggc aagctggcgc ggataaaggg gggatgggct gcccatgcaa
     1381 cgcctaagcc tgacgtgatt gggaataatt gcattggccg agaaaagcga acatgagttc
     1441 cgttgcagtt gaagatttcg caaaatattt ggcagacaaa cattttctgc tttcggacac
     1501 ggaatttcac tccgcgtcct ctagcgcgtc caacgccgaa cgatcccttc gcaaactttg
     1561 ggagtcgacg gagctcaccg cgaatggatt tgccgatgaa gtcgcccaat tttatggctt
     1621 gaagcggctc accttgcccc agctcgttgc cgcgccttcc ttgaccggac gcttttcgcc
     1681 gcgcttcttg cgcgacaccg cggtcttccc cttcgaactg ccgaaaggtc aatataagct
     1741 tgccgtcgcc gacccgagcg acgccgccgc tgtccgcgcc gccgaaatcg tccttggcac
     1801 gccggttttg gtcgagatcg cctccttcga ggatatcgcg actgttctcg gcgagcagct
     1861 tggcggcgag gagacaacgg ccggggatgg agatgccctg ggcgcccgcg ccgaggacga
     1921 cattgaaagt ctccgcgatc tggcgagcgg cgccccggtg gtgcgcgcgg tcaacgactt
     1981 gctcgaaaaa gccctagaac ttcgggccag tgatattcac atcgaaccgt tccggaacgg
     2041 ccttgtggtg cgaatgcggg tcgatggttt gctccgcgcc gttgccgcgc cggccaatgc
     2101 cttgccgcaa gccctgatct cccggatcaa gattctggcc ggcctcaaca tcgccgaacg
     2161 gcgcctgccg caagatggcg cggcaaggca gaaggtcgca cgctctgaaa tcgatattcg
     2221 cgtcgccacg atgccgtccc agcacggcga atcggccgtc atccgcctat tgccgcgcga
     2281 tcgaggtttg ctcgacgtgg caaaactcgg cttgtcgatc gccgatgaga aaatcgtgcg
     2341 ccggctgctt gcgctcccgc atggcatgat catcttgacg ggaccgacgg gcagcggcaa
     2401 gacgacgacc cttgcgacta tgctttcgat cctgaacgac ccatcgcgca agattctcac
     2461 gatcgaggat ccggtcgaat atgagatcca gggcgtgaat cagtcgcagg tgaaacccgc
     2521 gatcgggctt actttcgcca ccgcgatgcg cgctttcgtc cggcaagatc cagatgtcat
     2581 catggtgggc gaggtgcgcg atgccgagac cgcgaacatc gccgtgcatg ccgcgctcac
     2641 cggccatctc gtgctgacca cgcttcacac ggaaaatgcg gcggcggcgg tgccgcgcct
     2701 gctcgatctt ggggtcgagg gtttcttgct caaatcgacg ttgcgggcgg tgatcgcgca
     2761 acgcctggtt cgcatccttt gcgaccggtg caagcgtaaa cgcattctca cgtccggcga
     2821 tcttgccgcc gatccccgct tcgatgcgct tggcctgcgg tcaggcgatt gcgtttacga
     2881 accggctggc tgcgagcgct gcggcggcgc cggctatcgc ggccgcaatg gggtattcga
     2941 ggcgcttgaa ttgaaggacg aagtgtacga acttgtcgga ccgcacgccg atgcgcgcgc
     3001 gatcgatctc gccgcaagac act
//
LOCUS       DATMPU010000228         2160 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_3897224,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000228 DATMPU010000000
VERSION     DATMPU010000228.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 2160)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2160)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2160
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3897224"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            68..337
                     /locus_tag="VNY06_05205"
     CDS             68..337
                     /locus_tag="VNY06_05205"
                     /inference="COORDINATES: protein motif:HMM:NF018437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3D domain-containing protein"
                     /protein_id="HWX14241.1"
                     /translation="MTRKTISSALRSIAIDRTIWSCRLPFWLSTDLPWYNALPSPFRR
                     LMIAQDKGSAIVGPARCYIFFGSGDDAGARAGDIQQGRGLRSSAP"
     gene            549..905
                     /locus_tag="VNY06_05210"
     CDS             549..905
                     /locus_tag="VNY06_05210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012330834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase family protein"
                     /protein_id="HWX14242.1"
                     /translation="MAESAFAALPERFRQLCDGVVIRVEDFADDETLDALNCRSEFDL
                     LGLFRGQGRAQCGAAPVTGQFPNMVWLYRRPILDYWAQHESTFGEIVTHVLVHEIGHH
                     FGLSDDDMQRIEETAE"
     gene            complement(1231..1492)
                     /locus_tag="VNY06_05215"
                     /pseudo
     CDS             complement(1231..1492)
                     /locus_tag="VNY06_05215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012046649.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase, copper-containing"
     gene            1744..2094
                     /locus_tag="VNY06_05220"
     CDS             1744..2094
                     /locus_tag="VNY06_05220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX14243.1"
                     /translation="MPWWAPWVGTGVVFVVVATALWWWVPKWQANRLRLKIRDPKARA
                     DVEDNFRKTIGQLIGGAAVLVGTGFAYYQSQLAQQATHDQLTRSALNFPFVARASLSR
                     AMRSGKCAASSRRS"
BASE COUNT          444 a          613 c          614 g          489 t
ORIGIN      
        1 cgcgaggcgg ccagccagat caagcgtagt ggcaatttgt tccccagcct tcaactcgaa
       61 aggacctatg acacgaaaga ccatatctag tgcattgcgc tcaattgcca tcgaccggac
      121 gatctggagt tgtcggctgc cattttggct ttccacggac ctgccttggt acaacgcgtt
      181 gccttcgccg ttccggcggc tgatgatcgc gcaggacaag ggttcggcta tagttggccc
      241 agcccggtgc tatattttct ttggttcagg ggatgacgcg ggcgcacggg cgggcgatat
      301 tcaacaaggg cgaggtctac gttcttctgc cccgtgacaa ggctccggat tcgtgattga
      361 aaattccccc tttccaacca agcctccacg tttgagtgcg gcagatcttg agctgtggct
      421 tgacgctacg aggagcgtta tccgcgtctc aggcgccgca gccaatcaac gaccaacaat
      481 tcactctcgt gaataggaaa cgagagatcc atagcctggc ggcgccttcg cttgctgagt
      541 tcgagctcat ggcggaatcg gcgttcgccg cccttcccga acgttttcgc cagctttgcg
      601 acggggtggt gatccgcgtc gaggattttg ctgacgatga aacgctcgat gcgttgaact
      661 gcagaagcga atttgatctc ttgggcctat ttcgcggcca aggtcgcgcg cagtgcgggg
      721 cagctcccgt gactgggcaa ttccccaata tggtgtggct ttaccgccgc ccaatcctcg
      781 actactgggc acagcatgaa agcacgtttg gcgaaatagt cactcatgtc ctcgtgcatg
      841 aaatcggtca tcacttcgga ctttctgacg atgacatgca gcggatcgag gagaccgccg
      901 agtagtccgc aaggatcgtg atgcgagcca tcttcacata accccggagt tcctcatact
      961 tggtgccgcg cttgccgccg ccagtcagat tttgacgcca ccgcgcaccg agtgcagatg
     1021 attcccagag cttcgcaaac gttactgggg acgccacttc tgggcgagag gctacttctg
     1081 caagtggccg ttaccgcgct taacctcata agctcggatc gtttgatcgg cgctgtcgaa
     1141 tgcaatcatg tcgacccgaa ggggccgcaa gcacaggggc gtcggcatag atgccgatcc
     1201 ttcgttccta cttgtccgct gaatattccg tccaaccttg ccctcgaata caacatgcgt
     1261 gggaatgagc ttgcgcatga gttcgagcgt cttcggaaag tattcgcttg cctcggtaaa
     1321 gctctgaaat ttaccatcgc cgccgcgcgg aacataaaaa atcattttca ccgatgtagt
     1381 aggcgcgatc gtaatgcagg agcttaccgg ccgcgtccct caagccctca cgcggcagaa
     1441 ccatgactgc cccgcccatg ccagacacga caggcaatgg aatcatcgaa ccgctcgacc
     1501 caattaataa gcttggccgt atatagaccc cggttttcgg gacacttcgg ctgttctttg
     1561 tgtggtagtt ttaccgattc cgcttgggaa cgtcttgcaa ataaccgatt ttgggcgtga
     1621 ttttctgatg cacctgacta aacggcgatt agccgaaaac aagtttgggt aggtccgcga
     1681 ttgaaaagcg cctcgatttg accgaggcgt gagacggtcc ctttgcagtt tggaggcgga
     1741 acaatgccct ggtgggcgcc gtgggtaggg acgggggtgg tctttgtcgt ggtcgcgacg
     1801 gcgctctggt ggtgggtgcc aaaatggcaa gccaaccgcc tacgtctgaa aatccgcgat
     1861 ccgaaggcgc gcgccgatgt cgaggataat tttcgcaaga ccatcggtca gcttatcggc
     1921 ggcgcggcgg tgctcgtagg cactggattc gcctattacc aatcacagct ggcgcaacaa
     1981 gcgacccatg atcaactcac ccggagcgca cttaacttcc cgtttgtcgc tcgcgcgtcc
     2041 cttagcagag cgatgcggag cgggaagtgc gcagcgtctt ctcgtcgatc ttgagctgct
     2101 cgcaggaaac tgtcgagcca atcggcttcc cgaggcaagt ggacgtgtcc ccttcgatct
//
LOCUS       DATMPU010000229        10910 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_3275042,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000229 DATMPU010000000
VERSION     DATMPU010000229.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 10910)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10910)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10910
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3275042"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..310)
                     /locus_tag="VNY06_05225"
     CDS             complement(<1..310)
                     /locus_tag="VNY06_05225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012454518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX14244.1"
                     /translation="MNESFFASKSFSFTLVAAAIALVAASTLIFFVFRAVFGRRIRLP
                     RNGRTRPVRLAIIDAFDLDRQRQLVIVRRDNVEHLLMIGGPNDLVIESQIIRSESRES
                     R"
     gene            730..3234
                     /locus_tag="VNY06_05230"
     CDS             730..3234
                     /locus_tag="VNY06_05230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012592485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWX14245.1"
                     /translation="MSERQAYAGLHGAGRSGNLALTFASAVVLVTAVAAFSFLPNEAG
                     TKIAAVLFALFAIAGICGVFFFAVGLFQFSSQLTKSVVGKQICDGSSDGLVVTGAGGK
                     IIYANEVYFALASASGLADVRPVERLFARSPEASEAIYRLSQAAREGRSAIEEIRVSP
                     SLGGESAAGWYKIKVKPLPLAGVGRASLWSICDVSHERQRQENVFQELRQAIDFLDHA
                     PAGFFSCGSTGDVSYINATLAAWLGYELADAELGKLKLADFAASDGATLVASVSGGTG
                     EVRTEQFDTHLRCRDKQRLPVRLVHRVSFGHDGAASASRTLVLKRGIGEERAGDLIAG
                     EGRFAGVFAAMPMAVAVLGRNGQILCWNAAFARLMPEALKSTSDAAHSILSGILSRDH
                     AAIDAALAASFRFSSAIPPVDVGLAGKGDRSARLFFAAASEQAGNGATIYALDTTEQR
                     ALQSNFAQSNKMQAIGQLAGGVAHDFNNVLTAIIGHCDLLLANHRPTDPSFRDINQIK
                     QNANRAASLVRQLLAFSRRQTLRPQILQLGDVLADLQEMMKRLVGENITLELRHGRDL
                     WLVKADLHQFEQVLINLIVNSRDAMSRGGKIVLRTRNVETPDCGNFNEPSLVPNDYVA
                     IEVEDFGHGIPPDVRDKIFEPFFTTKEVGKGTGLGLATVYGIVKQTEGYVFCSSTVGK
                     GTTFTVLLPRYIPDESAASQPIEQVKTPSADLTGRGTILLVEDEEAVRAFGARALASR
                     GYTVLQAASGIEALQIVEEQDGKIDLVVSDVMMPEMDGPSMFTELRKRGVKARVIFVS
                     GYADKPFENLPDGEDFGFLPKPFSLKQLIEAVKGKR"
     gene            complement(3346..3672)
                     /locus_tag="VNY06_05235"
     CDS             complement(3346..3672)
                     /locus_tag="VNY06_05235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX14246.1"
                     /translation="MKSSLKVILAAGIGFGALGLAAKSVSAMPMSGLDPALAAPADTA
                     KSVEAVRWICGPYRCRRAPGWRRRYWWGPGYGFYGPGPWWRYRYGYYPWWPHMGWSPY
                     RYGYYW"
     gene            complement(3938..4948)
                     /locus_tag="VNY06_05240"
     CDS             complement(3938..4948)
                     /locus_tag="VNY06_05240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019401432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HWX14247.1"
                     /translation="MLKKIVIAFAVLFVLASGAAADTLSELKKRGLLVCGSNPGLAGF
                     GLPDGHGHWAGFDVDFCRAIAAAIFGDAGKVKFVPLAPKDRLQALGSGQIDVLASNTT
                     WTLSRETGQDLLFTGINYYDGQGFMVPKKLNITSALELSGTSICVQQSTTSELNLADF
                     FQVNGMTYKPVAFATADEAVKAYDNGKCQVYTADASDLYAGRMALADPDADIVLPEII
                     SKEPLGPVVRQGDDHWFNIVKWVNFAMVNAEEFGVTSKNIDEKAKSESPDIRRLLGFE
                     GNFGEAMGLESDWAYRIVKLVGNYGEVFERNLGENSPLNIKRGMNALWTKGGLLYAPP
                     VR"
     gene            5097..6284
                     /gene="metC"
                     /locus_tag="VNY06_05245"
     CDS             5097..6284
                     /gene="metC"
                     /locus_tag="VNY06_05245"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012590724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004121 - cystathionine beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HWX14248.1"
                     /translation="MAETTRQDRTALKNRTKLLHAGRRPSEQLGFVNTPVYRGSTVLF
                     PTLEDFYARKATFTYGTQGTPTTEALESAWTEICGAAGTVLAPTGLAAISLALLTAAK
                     SGDHILVTDAVYRPSRNFCDTVLARMGIETSYYDPALGAAIESLLRPNTTVIFLESPG
                     SQTFEVQDVPAITALARSKGICTILDNTWATALFFPPHVRGADMAVEAGTKYLSGHSD
                     LLIGLVSANAEWFPKLRATFDAFAMCAGPEDVFLSLRGLRTLDLRLREAERQGRDLAH
                     WLSARKEVIEVLHPALPCCPGHDIWKRDFLGSSGLFSVLLAPCSRAALGSMLDGLKLF
                     GMGYSWGGYESLVIPFDCRSYRSATAWNPKGPALRFSIGLEDIEDLKEDLDAGFARMR
                     ATS"
     gene            complement(6333..7001)
                     /gene="yihA"
                     /locus_tag="VNY06_05250"
     CDS             complement(6333..7001)
                     /gene="yihA"
                     /locus_tag="VNY06_05250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012386236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HWX14249.1"
                     /translation="MENLACAPPDYFELGRKLFARECAFAWAVADGAQLPNPVAPEIA
                     FAGRSNVGKSSLLNALTARKSLARISRTPGRTRELHFFALGGNSEKAELYLVDMPGYG
                     YAAASKELAASWARLASDFLRSRAALLRVMLLIDGRHGAKSVDVETMNLLDRAAVSYQ
                     IVLTKKDKVKAIEQEARLAATYDLIAKRPAAFPELIFTSSRSGEGIGALRAAIAKLLG
                     ERGR"
     gene            complement(7021..8850)
                     /gene="yidC"
                     /locus_tag="VNY06_05255"
     CDS             complement(7021..8850)
                     /gene="yidC"
                     /locus_tag="VNY06_05255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012590240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HWX14250.1"
                     /translation="MGKETRNVIIATCLSLLVVLAWDKFYAAPHFEKQRQIQAEMQAR
                     QAQSAQAPGQQASPSAEPKGSHSNTPAPAAPAVVKKSREEALAESPRVKIDAPAIYGS
                     IALKGARVDDVSFKDYRETVDPKSPNIILLSPLGSPYPYYAEWGYISDPGTSLILPGP
                     DTLWQADRDTLTEQSPVTLTFDNGQGLVFRRRIAIDDRYMFTVTDSVENKTAQPATIH
                     PYAFVKRHGRPESAYYSVLHEGFVGIIGDSGIQQIKYANIEKEEHASKSFGGTRGWLG
                     LTDKYWAAVVIPDQSKAVEANFSGSGGTPMIYQAGFVGSEGSTIAPGTSGEFATRIFA
                     GAKEVHTLDKYQIGLGIEKFDLLIDWGWFYFITKPMFRLLHFIYNFVGNFGVAILIIT
                     VLVKGLFFPLANRSYMAMAKMKAMQPQIIAIRDRFPDDKQKQQMETMALYKREKINPL
                     SGCLPLLVQIPVFFALYKVLVITIEMRQAPFFGWIKDLSRPDPTNVFNLFGLIPYDPT
                     QVPILGPYLAIGVLPLIMGITMFIQMKMNPEPTDPIQKQAFAWLPVVFTFTLGSFASG
                     LIIYWSWSNILSVIQQAAMMKKAGAKIELWDNLVGIFRKKATT"
     gene            complement(8867..9508)
                     /gene="rnpA"
                     /locus_tag="VNY06_05260"
     CDS             complement(8867..9508)
                     /gene="rnpA"
                     /locus_tag="VNY06_05260"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HWX14251.1"
                     /translation="MSFRRQSVAAGSVRNNDFKRKNEQVVRFAELKSKQEPRGARGAQ
                     ALIVESTGFSGKLMAKAPLRLKRRADFLRARQGTRAHARGLVLQAVPRLPTRRDIELA
                     ELSAPADSAATGGQSGQAQTLIPSNGGSHPDVQPRFGFTVSKRCGGAVRRNRIRRRLK
                     EALRLLNPLPARLGYDYVIVARPEALDMGFPALQAELRRAFGRAGTLNSPKAQ"
     gene            complement(9629..9763)
                     /gene="rpmH"
                     /locus_tag="VNY06_05265"
     CDS             complement(9629..9763)
                     /gene="rpmH"
                     /locus_tag="VNY06_05265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010659431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HWX14252.1"
                     /translation="MKRTYQPSKLVRKRRHGFRARMATVGGRNVIAARRARGRKRLSA
                     "
     gene            10758..>10910
                     /locus_tag="VNY06_05270"
     CDS             10758..>10910
                     /locus_tag="VNY06_05270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020173431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase"
                     /protein_id="HWX14253.1"
                     /translation="MTTVDFAGFVDKLADVAGETILPFFRTALQAQDKSAGGVFDPVT
                     EADRAAE"
BASE COUNT         2368 a         3074 c         3091 g         2377 t
ORIGIN      
        1 tgcgcgactc acggctttcg gaccggatga tttgggattc gatcacaaga tcgttggggc
       61 cgccgatcat gagcaaatgt tcgacgttgt cgcgccggac aataacgagc tggcgctgac
      121 ggtcgaggtc gaaagcatca ataattgcga ggcggaccgg ccgtgtcctg ccgttgcgag
      181 gtaaccggat acggcgtccg aatactgcac gaaaaacaaa aaaaatcagt gtcgatgctg
      241 cgaccaaggc gattgctgcg gccacaagag tgaaactgaa actcttgctc gcgaaaaaac
      301 tttcgttcaa tcccgggctc cttagttcag gggaaaaata agcagcggga aaccaacata
      361 acacgttgcc gtccctatgg cgcgccggct agcaattagt taacgaaaat cgtcaagaat
      421 tggtatgctt attcgggcgg caggggcctg agtttatagt ttaagggatt tacgcgaagg
      481 cgccgcgaga ttatagcgcc aagctggcgt tgcgatttga atatcatcgc ggcaaggtcg
      541 gggaggttcg ccgtaaggtc tggggatcac acccgccaag ggcgcgccgc actgtgtcga
      601 atcggcgaac cgagctaagc gatataatag ctcagctgct ggtcacggcg aaaggccaaa
      661 aatggtgagg tgacacgctg ttaccgaatg cgatttgggt cgcttttatt cttaattggg
      721 tgaattttca tgagtgagcg gcaggcttac gctggcctcc acggggcagg gcgcagtgga
      781 aacttagcct taacctttgc ctcagccgta gttttggtaa cagccgttgc ggctttttcc
      841 tttctgccca atgaggccgg cacgaagatc gccgctgtct tgtttgccct cttcgcgatt
      901 gcgggcattt gcggtgtttt cttctttgcg gtgggccttt tccagttttc cagccaattg
      961 acgaagagcg ttgtcggaaa gcagatttgc gacgggagca gtgacgggct ggtcgtgacc
     1021 ggcgccggag gcaaaattat ttatgcgaat gaggtctatt ttgctctagc gagcgcaagc
     1081 gggcttgccg acgtccgccc agtcgagcgg ctgtttgcga gatcaccgga ggcttccgag
     1141 gccatataca ggctctccca agcggcccgc gaaggcagat cagctataga agagatacgt
     1201 gtctctccgt cgcttggcgg cgagagtgcg gccgggtggt acaagatcaa ggtgaagccg
     1261 ctgccattgg cgggcgttgg gcgcgccagc ctctggtcca tctgcgatgt ttcgcatgaa
     1321 cggcaaagac aggaaaatgt ctttcaagaa ttgcggcaag caatcgattt cctggaccat
     1381 gccccggcgg gatttttttc ctgcggctca accggggacg tttcctatat aaatgcaacg
     1441 cttgccgcct ggctcggcta cgagctcgcc gacgccgaat tgggcaagct caagctcgca
     1501 gatttcgcgg ccagcgatgg cgcgacgctt gtcgcttccg tttctggcgg tactggcgag
     1561 gtccgcaccg aacagttcga cacccacttg aggtgccggg ataagcaacg ccttcccgtg
     1621 cgtctggtcc accgtgtgtc gttcggacac gacggcgcgg cttcggcatc gcgcacgctc
     1681 gtgctcaagc gcggaattgg cgaggaaaga gcgggagacc tgatcgctgg agaggggcgc
     1741 tttgcgggcg tttttgctgc aatgccaatg gcggtcgccg tcttgggcag gaacggacaa
     1801 attctgtgtt ggaatgctgc tttcgcgcgc ctcatgccag aggccttaaa atccaccagt
     1861 gacgctgctc attcgatcct ttcgggaatt ctcagcaggg atcacgccgc gatcgatgcc
     1921 gcacttgctg cgtccttccg cttttcttcc gctatcccgc ctgtcgatgt cgggcttgcg
     1981 gggaaagggg atcggtcggc gaggttgttt ttcgcggcgg caagcgagca ggctggaaat
     2041 ggcgccacaa tttacgcgct ggacacgacc gagcagcggg ctctacagag caatttcgcc
     2101 cagtcaaata agatgcaagc gatcggtcag cttgccggcg gagtggcgca tgatttcaac
     2161 aatgtcttga ccgcgatcat cggccattgc gatctgctgc tcgccaacca caggccgacg
     2221 gacccatcgt tccgggatat taatcagatc aagcaaaacg ccaatcgtgc ggcctctctt
     2281 gtgcgccagc tgctggcctt ttcccgccgc caaaccttgc ggccgcaaat cctgcaattg
     2341 ggtgacgtgc tcgctgatct gcaggaaatg atgaaacggc ttgtcggcga aaacatcacc
     2401 ctcgagctgc gtcatggacg cgatctttgg cttgtcaagg cggatctcca tcagtttgaa
     2461 caggtgctca tcaatctgat cgtcaattct cgcgatgcga tgagccgggg cggcaagatt
     2521 gtcttgcgta cgcgcaatgt cgagaccccc gactgcggga atttcaatga gccatcgctg
     2581 gttcccaacg actacgtggc tattgaggtc gaagatttcg gccacggtat cccgcccgac
     2641 gtgagagaca agatttttga accgtttttt accaccaagg aggttggcaa gggcacgggt
     2701 cttggccttg cgactgttta tggcatcgtc aagcagaccg aaggctatgt tttctgttcg
     2761 agcaccgttg gcaaagggac gacatttacg gttcttctgc cgcgttatat ccccgatgaa
     2821 tccgctgcgt cccagccgat agagcaggtg aaaacgcctt ccgccgatct cacggggcgc
     2881 ggaacaattc tcctcgttga ggacgaagag gcggtccggg cgttcggcgc ccgcgccctc
     2941 gcctcgcgcg gctacacagt gttgcaggcg gcctctggca tcgaggcgct gcaaatcgtc
     3001 gaagaacagg atggcaagat cgatcttgtc gtttcggacg tcatgatgcc cgagatggat
     3061 ggcccaagca tgtttactga attgcgcaaa cgcggggtca aggccagggt aatatttgtg
     3121 tccggatatg ccgacaaacc tttcgagaat ctgccggacg gcgaggattt tggtttcctg
     3181 ccgaaaccct tcagcctgaa gcaattgatt gaagccgtca aaggcaagcg ctgaggcagt
     3241 gatgaaaatg atccggcacg gggcacgcac cggggtctta aataaccggg caggcgcagg
     3301 aaggatcgat ttaatgaatt gcacagggct tatccgagcc tagacttacc aatagtagcc
     3361 atagcggtaa ggcgaccagc ccatatgagg ccaccagggg taatagccat atctgtagcg
     3421 ccaccaaggt cctggaccat aaaagccata gcccggaccc caccaatagc gccgccgcca
     3481 gccgggagcc cgacggcagc gataagggcc gcagatccag cggaccgcct ccacactctt
     3541 tgccgtatct gctggcgcgg cgagcgccgg atcaagtccg ctcatcggca tggctgagac
     3601 cgattttgcg gcaaggccaa gcgcgccaaa tccaatgcct gcggctaaaa ttactttgag
     3661 acttgatttc atatcatcct ccacccgctg acctacctga ttggtgctgc aacggagaaa
     3721 ctgctacata ggttctgaag cactgctgac caaaccattc atataccatt taacttcgct
     3781 accgccgccc cgttcccggc aatttcaagg cggttctgcg ggatttcgcg ggccttgccc
     3841 ggcagagccc aatggcgaga ttttgctctt gagcttatgc cgcaggcgaa ttgttgcaac
     3901 cagaatttac gcgcggctcg cacttcgtcg ccgcgctcta gcggaccggc ggcgcataga
     3961 gaaggccgcc tttggtccaa agcgcattca tgccgcgctt gatatttagg ggcgagtttt
     4021 cgccgagatt gcgctcgaaa acctcgccat aattgccgac gagttttaca atccgatacg
     4081 cccaatcgga ctcaagcccc atcgcttcgc caaaattacc ttcaaatccg agcaggcgtc
     4141 tgatatctgg gctttcggat tttgcctttt catcaatatt cttcgacgtc acgccgaatt
     4201 cctcggcgtt gaccatggca aaattgaccc attttacgat attgaaccaa tggtcatccc
     4261 cttgccgcac caccggcccg agcggctcct tggagataat ctcgggcagc acgatatcgg
     4321 cgtcgggatc ggcaagcgcc atgcgaccgg cataaagatc tgaggcatcg gccgtataga
     4381 cttggcattt cccgttgtca taggccttga cggcttcatc cgccgtggca aaggccacgg
     4441 gcttgtaggt catgccgttg acttgaaaaa aatcagcaag attgagctcg gatgttgtgc
     4501 tctgctgcac gcaaatcgat gtgccggaga gttccagagc cgaggtaata ttgagcttct
     4561 tcggcaccat aaaaccctgt ccgtcgtaat aattgatacc ggtaaaaagc aagtcctgac
     4621 cggtctcgcg cgacagcgtc catgtggtgt tgcttgccaa aacatcgatt tggcccgacc
     4681 ccagcgcctg caaccggtcc ttgggcgcaa ggggaacgaa tttgaccttg cccgcatcac
     4741 cgaagattgc agcggcgatc gcccggcaaa agtcgacgtc gaacccggcc caatggccgt
     4801 gcccatccgg caacccgaag ccagcaagcc ctggatttga tccgcagaca agaaggcctc
     4861 gtttcttgag ctcagagagc gtgtcggccg ctgcccccga agcaagaacg aaaaggacag
     4921 cgaacgcaat gacgattttt ttgagcatat gtatcgcttc ctaaatggtc cggatgaata
     4981 agagctccgc gccggggccg ggagattgca accggaggga ccgcttttca atgccaagct
     5041 tgacggcacg aggatcaaaa tcgcaagttt gcggcgcata tttgactgga gacaaaatgg
     5101 cagaaaccac gcggcaagac cgtaccgctc tgaagaaccg gacgaaattg cttcatgccg
     5161 ggcgccgccc gagtgaacag ctgggctttg tcaacacccc tgtctatcgc ggctcgacgg
     5221 tgcttttccc cactttggag gatttttatg cccgcaaagc aacgttcacc tatggcacac
     5281 agggaacgcc cacaaccgag gcgctcgaaa gcgcatggac ggaaatttgc ggcgccgctg
     5341 gaaccgtact cgcgccaacg ggtcttgctg caattagctt agccctgctc accgcagcca
     5401 aatccggcga tcatattctc gtgaccgacg cggtctatcg cccatcgcgc aatttttgcg
     5461 acacggtttt ggcccgtatg ggcatcgaaa ccagctatta tgatcccgcg ctcggcgcag
     5521 cgatcgaatc cctcctccgg ccgaatacaa ccgtcatctt cctggagtcg ccaggatcgc
     5581 agactttcga agtccaagac gttccagcga tcaccgcgct cgcccggtcc aaggggattt
     5641 gcacaattct cgacaatacc tgggcaacag ctttgttttt cccgcctcat gtgcgcggcg
     5701 cagatatggc ggtcgaagcc gggacaaaat acctttccgg ccattccgat ttgctgatcg
     5761 gccttgtatc cgccaatgcg gaatggtttc caaaattgcg tgctaccttc gacgcctttg
     5821 cgatgtgtgc cggtcctgag gatgttttct tgagtttgcg cgggctgcgc acgcttgacc
     5881 tccggttgcg ggaggctgag cggcaaggac gcgaccttgc gcattggctg tcggcacgta
     5941 aagaggtcat agaagtcttg catccggccc ttccatgctg tccggggcat gatatttgga
     6001 agcgggattt tttaggatcg tcgggcctat tctcggtgtt gcttgctccc tgctcgcgcg
     6061 cggcgctggg ctccatgctc gacggcctaa agctctttgg catgggctat tcctggggcg
     6121 gctatgaaag ccttgtcatt ccgttcgatt gccggagtta tcgcagcgcg acggcgtgga
     6181 atcctaaagg ccccgcgctg cggttttcca taggccttga agatatcgaa gatctgaaag
     6241 aagatcttga cgccggcttt gcgagaatgc gcgccacctc ttgagacatg cggctttaag
     6301 tggcatttcc aggcgtctca ctcctgctcg cctcatcgcc cgcgttcccc caataatttc
     6361 gcgatcgccg cgcgcaacgc gccaatgcct tctccactgc gagacgaagt aaatatcagc
     6421 tcgggaaagg cggcaggacg tttggcaatg agatcatatg tggcggcaag gcgcgcttct
     6481 tgctcgatcg ctttgacttt gtctttcttc gtcaggacga tctgatagga cacagcagcc
     6541 cggtcgagga gattcatcgt ctcgacgtcg acggattttg cgccatggcg gccgtcgata
     6601 agaagcatta cgcgcagcaa tgctgcgcgg ctgcgaagga aatcactcgc gagccgagcc
     6661 caggaagcgg ccaattcctt cgaggccgcc gcatagccgt aaccaggcat atcgacgaga
     6721 taaagttcgg ctttttctga attgccgccg agcgcaaaaa aatgcagctc gcgcgttcgc
     6781 cccggggtgc gcgaaatgcg cgccaacgac ttgcgggcgg ttaacgcatt aagaagcgag
     6841 gattttccaa cattggagcg cccggcgaag gcgatttccg gcgcaaccgg atttggcaat
     6901 tgcgctccgt ccgctactgc ccaagcaaac gcacattccc gcgcgaaaag tttgcggccc
     6961 aattcgaaat aatctggcgg agcacacgcc aaattttcca tgctccgcgg tcccgccggc
     7021 tcaggttgtc gctttcttgc ggaatatgcc gaccaaattg tcccaaagtt cgatcttggc
     7081 gccagccttc ttcatcatcg ccgcctgctg gatgaccgac aaaatattgc tccagctcca
     7141 atagatgata aggccagagg caaagctgcc gagcgtgaag gtgaagacaa ccggcagcca
     7201 ggcgaacgcc tgcttttgta tcgggtcggt cggctctggg ttcatcttca tctgaataaa
     7261 catggtgatg cccatgatga gcggcaaaac accgatggca agataggggc ccaatattgg
     7321 aacttgcgtc ggatcatagg gaatgaggcc gaacagattg aagacattgg ttgggtcggg
     7381 acgcgagaga tccttgatcc agccaaaaaa cggtgcctgc ctcatctcaa tcgtaatgac
     7441 gagcaccttg tagagtgcga agaacaccgg aatctgtaca agcagcggca agcaaccaga
     7501 caggggatta atcttttcgc gcttgtaaag cgccatggtt tccatctgct gcttttgttt
     7561 gtcgtcggga aagcggtcac ggatcgcgat gatttgcggc tgcatggctt tcatcttggc
     7621 catcgccata taactgcgat tggcgagcgg aaaaaagagg cccttgacca gaacggtaat
     7681 gatcaggatc gcgacgccaa aattgccgac aaagttatag ataaaatgga gcaagcggaa
     7741 catcggcttg gtaataaaat aaaaccagcc ccagtcgatc agcagatcga atttctcgat
     7801 acccagacca atttgatatt tgtcgagcgt gtggacctct ttggcgccag caaaaatcct
     7861 ggtcgcgaac tcgcctgatg tcccaggtgc gattgtactt ccttcagatc caacaaaacc
     7921 ggcttggtaa atcatgggcg tgccaccaga cccggaaaaa tttgcttcca cggctttgct
     7981 ctggtccgga atgacgacgg ctgcccaata tttgtcggta aggccgagcc agccacgggt
     8041 gccaccgaaa ctttttgagg cgtgttcttc cttttcgata ttggcgtact ttatttgctg
     8101 aatgccggaa tctccgatga ttccgacgaa gccttcgtgc aggacggagt agtaggcaga
     8161 ctcaggtctg ccgtggcgtt tgacaaatgc atagggatga attgtagcgg gctgcgcagt
     8221 tttgttttcg acgctatcgg taaccgtaaa catgtaacga tcatcgattg caatcctgcg
     8281 gcgaaaaaca agcccctgcc cattgtcaaa ggtaagggtc acaggcgact gttccgtaag
     8341 cgtgtcgcga tcggcttgcc ataaggtgtc tggtcctggc aagataagcg aagtgccggg
     8401 atcggaaata tagccccact cggcataata aggataaggc gatcccaagg gcgagagcag
     8461 gataatgttc gggcttttgg ggtcaacggt ctcacgataa tccttaaacg acacatcgtc
     8521 gactcgcgcg cctttcagcg caatcgatcc gtagatggca ggagcgtcga tcttgacgcg
     8581 aggactttcg gctaatgcct cctcgcgaga ctttttcact actgcgggag ccgccggagc
     8641 tggcgtattg gaatgagatc cttttggctc cgcggacggg cttgcttgct gacccggcgc
     8701 ctgcgcgctt tgcgcttggc gtgcctgcat ttcggcttga atctggcgct gcttctcaaa
     8761 gtgcggcgcg gcgtaaaact tgtcccaagc gagaacgacg agcagcgaca gacaggtcgc
     8821 gatgatgacg ttcctggttt ctttgcccat cttctcgatg tcctgcctat tgagctttgg
     8881 gactattcaa agtcccggcc ctgccgaatg cacgccggag ctccgcctgc aaagcaggga
     8941 agcccatgtc gagcgcctcg ggccgggcaa cgattacata atcatagccg agccgcgctg
     9001 gtagcgggtt cagcagccgc agcgcttctt tcaggcggcg gcggatccgg ttgcgccgga
     9061 ccgcccctcc gcaacgtttg cttaccgtaa agccaaaccg cggctgcaca tcagggtgag
     9121 agccgccatt tgaaggaata agggtctgcg cctgcccgct ctggcctcca gttgctgctg
     9181 aatctgctgg tgcgcttagc tccgccagct ctatgtcccg tcgcgtaggg aggcgcggta
     9241 cggcttgcaa gaccaatccc cgcgcatggg cccgtgtgcc ctgccgagcg cgcaaaaaat
     9301 ccgctctccg tttcaatcgt aagggcgctt tggccatgag ctttccagag aaaccggtgg
     9361 actccacgat caaggcttgg gcgccgcgtg cgccgcgagg ctcttgcttg gacttcaatt
     9421 ccgcgaagcg aacgacctgc tcgttcttcc gcttaaaatc attatttcga accgagcctg
     9481 ccgcgacgct ctgcctgcgg aatgacatgg cttcccgccc gagcaatctg ggaaaacaag
     9541 gtaaggccgc ccccaatctt tcgaaccgga acagatggct ttccagtgcg ccgcggcggc
     9601 atccgaactc aagatttctt caagcatttt atgccgaaag ccgtttgcgg ccccgcgcgc
     9661 gccttgctgc aatcacattg cgccccccca cagtggccat acgggcgcga aacccgtgcc
     9721 ggcgcttgcg cacaagcttg cttggttgat atgtccgctt caccgcgctg ctccaatctc
     9781 tataaagtta ccagacggtg ctccagcggg acctgaactt tcaggcgccg ccaagaccga
     9841 cgattacgcc acacaaacaa aaacggtcct ctccgcccat gagatcggag aaggtttcac
     9901 cgatgcctga aaattggctc cttataggag caaccgccaa accaagtcaa ttcggcgaaa
     9961 tggctgagcg gcctccaacc gcgccgcaaa aaagcgtcaa atcccgccca gttttcccgg
    10021 agaggctgtt ggcggatgct atgcacaaga actgcgcaac gggtgcagag ttttggcatt
    10081 ttggcgcggg aaaactgccc cgccagcctc atggctacgg ctcctcaggc cgccgcaaga
    10141 gggacaagcc agagcaaggt gacgtttctt cgcaacgtca ccttactctg gatccttgtt
    10201 ttgccgcgtg tttggacgca aaaccggttc ccgcttttgc tggccacact atagcggcag
    10261 acttggcaca ttgattgcaa ttctttgacc tagaccgact ccctacggtc tgctccgggt
    10321 ctaacgcggg gcctgggaat taaatactgc cgctggcgga cctggaggtc cgccagcggc
    10381 ttttcttatg gtgattgcag cttgcgcatg aatctttggg cctccgcaca gagaggaggc
    10441 acagatggaa tagtaacggc cggcgcgctt atccctcgac gatgggacat gttttatgga
    10501 agtaacccgc ggccacgggc cggccaaatt gcggctcttg gctgcgcgga acacgcggta
    10561 aaacaaaact tgaaacgcaa aaatgtcctc ttgctgcgag caacggcttc gattttgcca
    10621 aatttctgcg ccgttcaaaa agttccggcg cgctccgcag gtcaaacgtc tggagtcaga
    10681 aattcctcgc cttttggctt gccgccaagc agaaggcgtg gcaacgagcg gcctcagccc
    10741 tctcggaatc agcagcaatg accactgtag atttcgccgg ttttgtcgac aaactcgccg
    10801 atgtggcagg cgaaacgatt ctcccgtttt ttcgtaccgc gctgcaggcg caagacaaga
    10861 gtgcaggagg cgtgttcgat ccggtaaccg aagccgaccg cgccgcagaa
//
LOCUS       DATMPU010000230         4982 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_3276939,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000230 DATMPU010000000
VERSION     DATMPU010000230.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 4982)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4982)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4982
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3276939"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(445..771)
                     /locus_tag="VNY06_05275"
     CDS             complement(445..771)
                     /locus_tag="VNY06_05275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012589560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HWX14254.1"
                     /translation="MVIYKRVRATLYPLLLYCLSGSIGTYFVWHAVNGERGLKTKDEY
                     ERKITALLGELDGLRSEHALWSHRVVLLSGRVIDRDLIDEEARALLGRVHRNDLVVFF
                     PRTQNE"
     gene            complement(924..3188)
                     /gene="ptsP"
                     /locus_tag="VNY06_05280"
     CDS             complement(924..3188)
                     /gene="ptsP"
                     /locus_tag="VNY06_05280"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012383896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HWX14255.1"
                     /translation="MYGTFGGPRLLLRRLREVMAEPVSPQARLDKIVVHIAASMVAEV
                     CSVYVLRADDKLELYATEGLNREAVHLTALRKGEGLVGLIAETAEPLALADAQLHPAF
                     SYRPETGEEIYSSFLGVPVLRGGNTLGVLVVQNKARRTYPEEEIEALQTTAMLLAEMI
                     ASGEVQLLAPPGKDIAFRGPVALKGTPVADGVGLGHIVLHEPRIIVKHIVAEDIRREN
                     QRLDDAIDAMRHSLDRLIESKSVGSGEHRDVLETFRLFAHDRGWLRRLREAVATGLTA
                     EAAVERVQNDTRAKLHRQTEPYLRERLHDLDDLANRLLHQLTGQDFVATHENLPSNSI
                     IVARAMGPAALLEYDRSKIRGLILEESGALSHVAIVARALGIAAVGNVPNIVDLAEAG
                     DAVIVDGVLGDVHLRPHPDVEHSYAEKARLRARKQAQYQKLRDVPSVTKDGVAIELHM
                     NAGMLVDLQHIEETGAVSVGLFRTEIQFMLASRLPKMSEQETLYRTVLDATGGKPITF
                     RTLDIGSDKVLPYLEKVEEENPALGWRAIRIGLDRPGLLRSQLRALVRAGSGRELRVM
                     FPMITTVEEFEIAKSFVDSELDHLRQHGRAPPSSLTLGVMIEVPSMLWQLDEICKRAD
                     FLSVGSNDLVQYIYATDRENKRVASRFDPLSTPVLQVLKIIADKARIHATPLTLCGEI
                     GGKPLEAMVLLALGYRGLSMSAASIGPVKAMLLATNLEETGEFVSSLMEEANAGQSLR
                     DKLRKFAKAKNIPV"
     gene            complement(3650..4888)
                     /locus_tag="VNY06_05285"
     CDS             complement(3650..4888)
                     /locus_tag="VNY06_05285"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012634396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HWX14256.1"
                     /translation="MSRLVLKFGGTSVANIERIRNVARHISREWKVGHDVAVVVSAMA
                     GKTNELVGWCEGAFKLCDRREYDAVVASGEQVTAGLLAIVLQEMGIPARSWQGWQVPI
                     LTSNEHGSGRIQHIESTAVIEGFAEQREVAVITGFQGLHKETGRIVTLGRGGSDTSAV
                     AVAAGIGADRCDIYTDVDGVYTTDPRIVPKARRLDRIAFEEMLEMASLGAKVLQVRSV
                     ELAMVQKVKTFVRSSFDDPDNFDQGTLICDEEDIVENQVVTGIAFSRDEAQITLRRVA
                     DRPGIAAAIFMPLAEANINVDMIVQVASEDSGVTDLTFTVSAADFARAQKILTDAQAQ
                     IGFTTLQGASDVVKISAIGVGMRSHAGVAARAFKALAEKGINIRAITTSEIKFSVLIE
                     AAYTELAVRTLHTLYGLDKA"
BASE COUNT         1123 a         1488 c         1329 g         1042 t
ORIGIN      
        1 acgcaaggcg cagtgctcct gctgcagctt gcgatccggc gaccagaaaa aaggatgaca
       61 taagaagaat gatcgatccg ccggtcacta taaagcggcg cagaaattgc tcttttgtac
      121 gactgatgtc aatcaacgcg cccactggag cctccatcac aaggccagag accgtagcgc
      181 atcacgatgc cgattttgtc ttctgtccaa tttcggtctg ataaaggtag accgcgagat
      241 aaggtccaag gccttgtcga acatccgcca aaaaactaag gcgtcgaggg aatagtcgta
      301 acgcattgct caagccgtcg agcagaatga cagcctcctg gactctcaca gtacccataa
      361 agttccaagc ccgacgaaac aaagaatagt actgccgtct gccacaagac cctccggccg
      421 cgagccgggc gctacttagc caggctactc attttgtgtc cgcgggaaaa acacaacaag
      481 gtcattgcga tgaacgcggc cgagaagtgc gcgtgcctct tcgtcgataa gatcacgatc
      541 gattacgcgc ccggacagca gcacaactcg gtggctccac aatgcatgct cagatcttag
      601 accatccaac tcaccaagca acgctgtgat cttgcgctca tattcatcct tggtcttaag
      661 gccacgctcg ccatttaccg cgtgccatac gaaataggtt ccgatcgagc cggacaggca
      721 ataaagcagc aagggataga gggtggcgcg cacgcgctta tagatgacca tgccgcaact
      781 taaaggtgat aggtaagcga tccgttaaat ttaggtttcg cagacccttg cctgctgacc
      841 cccatccact tttgggagca attttcggaa gcccggcacg ggcttcttgt gggagttcgc
      901 atggcgaagg cgatggatgg cgatcacaca ggaatgtttt tggcttttgc aaattttcgt
      961 aatttatcgc gcaaggactg ccccgcgttc gcttcttcca tcagcgacga cacaaactct
     1021 cccgtctcct cgagattggt ggcaagcaac atggccttca caggcccaat agacgcagcc
     1081 gacatcgata gaccacgata gcccaacgcc agcaaaacca tggcttccag cggtttgcca
     1141 ccgatttcgc cgcagagcgt cagcggcgtc gcgtggatgc gtgctttgtc ggcgataatt
     1201 ttaagaactt gcaggaccgg cgtagagagc ggatcgaacc gagaggctac ccgcttattt
     1261 tcgcggtcgg tcgcatagat atattgaact aggtcgtttg agccaaccga tagaaagtcg
     1321 gcccgcttgc aaatttcatc gagctgccag agcatcgacg gcacttcgat catgacgccg
     1381 agagtgaggc ttgagggtgg tgcgcgaccg tgctgccgta gatggtcgag ttcactatcc
     1441 acaaaggact tcgcgatttc aaattcctca accgttgtga tcatcggaaa catgacgcgc
     1501 aattctcgcc ctgagccagc gcgcaccaaa gcccgcagct gcgaccgcaa caagccaggc
     1561 cggtcaaggc cgatgcggat ggcacgccag cctagcgccg gattttcctc ctcaaccttc
     1621 tcgaggtaag gcaggacctt atccgacccg atatcaagcg tgcggaacgt gatgggcttt
     1681 ccgccagtcg catcgaggac ggttctataa agcgtctcct gctcgctcat ttttggcaag
     1741 cgcgatgcga gcatgaactg gatctcggtc cggaacaatc ccactgatac ggcgcctgtt
     1801 tcctcgatat gctgaagatc gacgagcatt cctgcattca tatgtaattc gatcgctaca
     1861 ccgtccttgg tcaccgatgg cacgtcgcgc agtttctggt attgcgcctg cttgcgcgca
     1921 cgaaggcgcg ccttttccgc gtaagaatgt tcgacatcgg gatgcgggcg gagatgaacg
     1981 tcaccaagga cgccatcaac aatgactgca tctcccgcct ccgccagatc gacgatgttg
     2041 ggcacattgc caaccgcagc aatgccgagc gcgcgcgcca cgatcgcaac atggctcaaa
     2101 gcgccggatt cctcaaggat aaggcctcga atcttcgacc ggtcatactc tagcaatgcc
     2161 gcagggccca tcgcacgcgc gacaatgatc gaattgctcg gcaaattctc atgtgtggca
     2221 acgaaatctt ggcccgttaa ctggtgcaac agcctgttag caagatcatc gagatcatgc
     2281 agccgctcgc gcaaataggg ctcggtctgg cgatggagct tggcgcgcgt atcgttttgc
     2341 acgcgttcca cggcagcttc agcggtgagc cccgtcgcca ctgcttcgcg cagcctgcgc
     2401 agccagcccc ggtcgtgcgc gaacagccgg aacgtctcaa gtacgtcgcg atgctcgccg
     2461 gaacctacac ttttgctttc gataaggcgg tcaagcgaat ggcgcatggc gtcaattgca
     2521 tcgtcgagcc tttggttttc ccgccggata tcttcggcaa caatatgttt gacgatgatg
     2581 cgcggctcat gcaggacaat atggccaagg ccgacgccgt ccgcgaccgg tgttcccttc
     2641 aaggcgaccg gcccacggaa ggcgatatct ttgccgggcg gcgcaagtaa ttgcacctca
     2701 ccagaggcaa tcatttcggc gagcagcatc gccgttgtct gaagcgcctc gatttcctct
     2761 tcgggatagg tgcggcgcgc cttgttctgg acgaccagca cgccgagcgt gttgcctcca
     2821 cgcagaaccg gcacacctaa gaaagaagaa taaatttcct caccggtttc cggtcggtat
     2881 gagaatgctg ggtgaagctg ggcatcagcc agcgccagtg gttcggcagt ctctgctatg
     2941 agaccgacga gcccttcgcc ctttcgcaga gcggtcaaat gaaccgcttc gcggttcaat
     3001 ccttcggtcg cataaagttc aagtttgtcg tcagcgcgca gcacgtaaac tgagcacact
     3061 tccgcgacca tgctcgcggc gatatgaaca acgatcttgt cgagcctggc ttgcgggctg
     3121 accggctccg ccatgacttc gcgaagacgg cgcagaagca gacgggggcc accgaacgtg
     3181 ccatacatga gcgtttctcg ttcacacctc agagtttcac aactcggctc agataattcg
     3241 acttgaccgg cagccaagcc ggctcatccg ccactgttcc atggattgca cctccgtggc
     3301 ttcctcacgg ccccaagcag gccagcttcg cctatccgag ccctatgagc ggggctcata
     3361 aagcccggtc gtgcgcaaac cgttcaaatt gacgtatgat cttcctcacg aaacttgctt
     3421 ccagctccgc agatcatctc caagtgttat cccgcttctc gcggaagcat gtgagaacta
     3481 tcggcacgtg ctatcgcaga cttcggaaat gcgccagccg ccccgctcta acgaatatgt
     3541 tggtctcaac aaactcgggg ttccggcctt cgtctattgg tccgtccagc tagcaaactc
     3601 cggaccaaga acgcaaaaaa aattggcggc cgggcggcgg cgctctggct caagctttgt
     3661 ccaggccata aagagtgtgc aatgtcctaa ctgccaattc cgtgtaggct gcttcgatca
     3721 aaaccgagaa tttaatttcg gaggtcgtga tcgccctgat gttgatgcct ttttcagcca
     3781 acgccttgaa cgcgcgtgcg gcgacaccgg catggctgcg catgcccact ccgatagcgg
     3841 aaatttttac aacatcactt gccccctgaa gagtggtaaa tccgatctgc gcttgtgcat
     3901 ctgtcaatat cttctgggct cgggcaaaat cggccgcaga cacagtaaaa gttagatcgg
     3961 tcacgcccga atcctcggat gctacctgaa cgatcatgtc gacattgata ttggcttcgg
     4021 cgagcggcat gaatatggcg gctgcgatgc cgggacggtc cgccacgcgg cgcaaggtga
     4081 tctgtgcctc gtcacgggag aaagcgatgc cggtcaccac ttggttctcc acgatatcct
     4141 cctcatcaca aatcaaggtg ccctgatcga aattgtccgg atcgtcgaag gacgaccgga
     4201 caaatgtctt gaccttttgt accatggcaa gttcgaccga gcggacctga agcaccttcg
     4261 ccccgagtga ggccatctcc agcatttcct caaaagctat gcgatcgaga cgtctggctt
     4321 ttgggacgat cctggggtcg gtcgtataga caccgtcaac atcggtatag atgtcgcagc
     4381 ggtctgcgcc gatcccagct gcaaccgcca ccgcgctcgt atccgaaccg ccccgcccga
     4441 gcgtgacgat acggcctgtc tccttatgga ggccttgaaa gccagtgatc accgcaacct
     4501 cacgctgctc tgcaaagccc tcaatgactg cggtggactc aatatgttga atgcgccccg
     4561 acccatgctc gttcgaggta aggatcggca cttgccagcc ctgccaagaa cgggcgggga
     4621 tgcccatttc ctgcagcacg atggcaagca ggccggcggt cacctgttca ccggaagcga
     4681 caacggcatc atactcccgc ctgtcacaga gcttgaaagc tccctcgcac cagccgacaa
     4741 gctcgttggt cttgccagcc atggcagaga ctacaaccgc cacgtcatga ccgaccttcc
     4801 attcgcggct aatgtgcctt gcaacattgc gaatgcgctc tatgtttgcg accgacgtgc
     4861 cgccaaactt caacacaaga cgggacatcg ggcttagacc gtctccttcg ggtcgtttgg
     4921 agttcgttca gaaaatgagc cccgccagag cggtgccggc ggacatacat gcaaatgccg
     4981 gc
//
LOCUS       DATMPU010000231         5228 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_1431585,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000231 DATMPU010000000
VERSION     DATMPU010000231.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 5228)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5228)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5228
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1431585"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            405..1442
                     /gene="obgE"
                     /locus_tag="VNY06_05290"
     CDS             405..1442
                     /gene="obgE"
                     /locus_tag="VNY06_05290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020177399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HWX14257.1"
                     /translation="MKFLDTAKVYIRSGDGGAGCLSFRREKFIEFGGPDGGDGGRGGD
                     VIAECIADLNTLADYRYRQHFKAGTGVHGMGKNRTGSKGADVILKVPPGTQIFDEEGE
                     VLLGDFTQAGQRVCLARGGNGGFGNARFKTSTNQAPRHANPGEKGEERIIWLRLKLIA
                     DVGIVGLPNAGKSTFLASVSAAKPKIADYPFSTIHPRLGVAAYNGREFVVADIPGLIE
                     GAHEGHGLGDRFLGHVERCRVLLHLVDATAGNAGEAYKTVRGELKAYGNGLPEKPEIV
                     ALTKIDSVDQETLATQVLRLKRGAKQTPLRLSAITGANVGETLRRLLTTIEEVQKSRT
                     AEPEVIAKWHP"
     gene            1457..2638
                     /gene="proB"
                     /locus_tag="VNY06_05295"
     CDS             1457..2638
                     /gene="proB"
                     /locus_tag="VNY06_05295"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012383425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HWX14258.1"
                     /translation="MPSMFLPPLPSLKNFRRVVIKVGSSLLIDRGQACVRNGWLESLA
                     KDIADLHANGADILVVSSGAIALGRTVLGLPERGLKLEDSQAAAAVGQIALARIWAQV
                     LADRKITAGQILVTFGDTEERQRYLNARATIGRLLDLRAVPVINENDTVATNEIRYGD
                     NDRLAARVATMASADLLILLSDVPGLYTAPPAENPKAELVPLVTHVDEKIEAMAGGAA
                     SQISRGGMRTKIEAAKIATNGGTHMLIADGRVEHPISRLNEPGPCTWFLSGSNPVTAR
                     KKWIAGSLEPRGVLYVDAGAAKAITNGKSLLPAGVTRVEGNFTTGDCVLIRDARAAEI
                     GRGLVGYDASEAASIAGRNSQAILSVLGSPGRAAIIHRDDMVLVGGYLAAVARGADGT
                     S"
     gene            2625..3923
                     /locus_tag="VNY06_05300"
     CDS             2625..3923
                     /locus_tag="VNY06_05300"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020177391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HWX14259.1"
                     /translation="MEHPKRIEIKDSKVNPAKLMAAMGRAARKCAHELSLAAPETKDR
                     ALRSAAAAIRARAKDILAANACDMAAAKDSGATGAFLDRLALDASRCEAMASGLEAIA
                     ALPDPVGRLLARFERPNGLLIERVATPLGVVGVIFESRPVVTADAGALCLKAGNAVIL
                     RGGSESFYSSRSIHDCLVAGLIETRLSCDAIALVPITDRAAVGEMLSGLSGNLDVIVP
                     RGGKDLVARVQAEARVPVFAHLEGIVHVFVDRDADLKKAKAILFNAKLRRTGICGAAE
                     TLLVDRVCAPTHLAPLVKMLLDAGCTVRGDGTTCAADPRVTPATEADWSTEYLDAIIS
                     VRVVDDLDEAIAHIETFGSRHTDCIVTENKERAGRFLREVDSAIVMHNASTQFADGGE
                     FGFGAEIGIATGRMHARGPVGLEQLTSFKYRVHGAGQTRA"
     gene            4295..4885
                     /locus_tag="VNY06_05305"
     CDS             4295..4885
                     /locus_tag="VNY06_05305"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016918140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HWX14260.1"
                     /translation="MPGLRVGLFGGSFNPPHDGHRAASLLALRRLSLDRVWWLVTPNN
                     PLKDARELAPLALRLEAARKVSRHPRIEVTAIEAAIGATYSFETISYLKRRCPSVHFV
                     WLMGADNFQYFDRWQRWREIAELVPIAIIDRPGFTLTATHGRAAVTLARNRLNETRAS
                     VLATVMPPALVFLHGPRSPKSSTELRRKLHCESKER"
BASE COUNT         1026 a         1468 c         1594 g         1140 t
ORIGIN      
        1 tgaccgcaag acgtgggcgg caaaccagat cgcagcaggt gtaagccggg ctacgccggc
       61 aatggcgcag cagcccctgg atgcaaatga gggcaaggtg cttgccccgg ctattgagcc
      121 attcgacatt tgatgacccc actcggcagg tctttcttgc tggcgaactg gtctggcgaa
      181 ttcagccctt gccctttcgc tctgccatga gctcaacaca ggtcccttgt cgtgccgtga
      241 gcaatggttt gtgctaagct gcgggatgga agtctgagtt cgttctcacg accataggat
      301 ccgcagcggc acattgaaag tgttgatcgt ttttttcgat tggggcattc tttgcactaa
      361 tgccgtccgc ccgccaggac tcagtttcaa ggccatgttt ttcgatgaag tttctcgata
      421 ccgccaaagt ctatatccgg tccggcgatg gcggcgctgg ttgcctgtcg tttcggcgtg
      481 agaaatttat cgaatttggc ggcccggatg gtggcgacgg cgggcgcggc ggcgatgtga
      541 ttgcggaatg tattgctgac ctcaacactt tggccgatta tcgctatcgg cagcatttca
      601 aggccgggac tggcgtgcat ggcatgggca agaatcgtac agggtccaag ggcgccgatg
      661 tgattctaaa ggttccgccc ggaacccaga ttttcgatga ggaaggagag gttttgctcg
      721 gcgacttcac gcaagccggg cagcgtgtct gtctcgcgcg cggcggcaat ggcggatttg
      781 gcaatgcccg cttcaagacc tcgaccaacc aggcaccacg ccacgccaat ccgggtgaga
      841 agggcgagga gcggatcatt tggctgcgtt tgaaactcat tgccgacgtt ggcatcgtcg
      901 gattgccgaa tgctggaaaa tcgactttcc ttgcctctgt cagcgccgca aaacctaaga
      961 tcgccgacta tcccttcagt accatccatc ctcggctcgg tgtcgccgca tataacggcc
     1021 gcgaatttgt tgtcgccgat attcctggcc tgatcgaagg ggcgcatgaa ggtcacggcc
     1081 tcggcgacag gtttttgggc catgtggaac gctgccgtgt gcttctccat cttgtggatg
     1141 ccacagccgg gaatgcgggt gaggcctata aaaccgtgcg cggcgagctc aaagcctatg
     1201 gaaatggcct tccggagaaa ccggaaatcg ttgcgctcac gaaaatcgac agtgtcgatc
     1261 aggagacatt ggcaacccaa gttctgcgtc tcaaacgcgg cgcgaaacag acaccattgc
     1321 gcctctcggc aattacaggc gctaatgtgg gggaaactct gcgcaggctt ttgaccacga
     1381 tcgaagaggt ccagaaatcc aggaccgcgg aaccggaagt tatcgcaaag tggcaccctt
     1441 gagcttggcc agccaaatgc cctcgatgtt tctcccgccg ttgccgagcc tcaaaaattt
     1501 ccggcgggtt gtcatcaaag tcggttcttc cctgttgatc gatcgcgggc aagcctgtgt
     1561 caggaatggc tggctcgaat cgctcgctaa ggatatcgcg gacttgcatg cgaatggcgc
     1621 ggatatcctt gtggtttcct cgggagcgat cgcgctcggg cgcacggtgc tgggacttcc
     1681 tgaacgggga cttaagcttg aagacagcca ggcggcggct gccgttgggc aaatcgcttt
     1741 ggcgcgaatt tgggcccagg tccttgcgga taggaaaatc acggcgggcc aaattctggt
     1801 taccttcggc gataccgagg agcggcagcg ctatcttaac gcaagggcaa cgattggccg
     1861 cctccttgac ttgcgcgccg tgccggtcat caacgagaac gatactgtgg ctacaaacga
     1921 aatacgttat ggcgacaatg atcgcttggc tgcccgggtc gccactatgg cgagcgccga
     1981 tctcttgatt ttgctttccg atgtacctgg gctatacacg gcccctccgg cagagaaccc
     2041 aaaggctgag cttgttccgc tcgtcacgca tgtggacgaa aagatcgagg cgatggcggg
     2101 cggcgctgcc tcgcagattt cgcgtggcgg tatgcgtacc aagatcgagg ccgccaaaat
     2161 cgcgacaaac ggcggcaccc atatgctcat cgccgatgga agggtcgagc atcccatttc
     2221 gcggctgaat gagcctggac cttgcacgtg gttcctgtct gggtccaatc cggttactgc
     2281 acgaaagaaa tggatcgccg gttcacttga gccgcgcggc gtcctctatg tcgatgccgg
     2341 agcggcgaag gcaatcacca atggcaagag tcttctcccc gcaggcgtga cgcgggtcga
     2401 ggggaatttc acaaccggcg actgtgtgct aattcgcgat gcccgcgccg ccgaaatcgg
     2461 gcgcgggctt gtcggctatg atgcaagtga ggcggcctcg atcgcaggac gcaattcgca
     2521 agcgatcctg agcgtccttg gttctccggg gcgcgcagcg atcattcatc gtgacgatat
     2581 ggtccttgtc ggcggctacc ttgccgcggt ggcgcggggt gcggatggaa catcctaagc
     2641 ggatcgagat taaagacagc aaggtgaacc ccgccaaact catggccgcg atggggcgcg
     2701 cggcacgcaa atgcgcacat gagctttcgc tggccgctcc cgagacaaag gatcgcgccc
     2761 tccgctccgc cgcggcggca atccgcgcgc gagccaagga tatcctcgcg gccaacgcgt
     2821 gcgacatggc tgcagccaag gacagtgggg cgacaggagc ttttctcgac cggcttgccc
     2881 tcgatgcttc ccggtgtgag gccatggcca gcggtcttga ggcgattgcc gcgcttcccg
     2941 atcccgtggg gcgcctcctc gcgcgctttg agcgtccaaa cgggctcctc atcgagcgtg
     3001 tcgcaactcc gctcggcgtc gtcggtgtca tctttgaaag ccggccagtc gtgacggcgg
     3061 atgctggagc gctctgcctt aaagccggca atgcggtcat cttgcgtggc ggctcggaga
     3121 gcttttattc cagccgctct attcatgatt gcctcgttgc gggattgatc gaaacccgtc
     3181 tgtcatgcga cgcaattgcc cttgtgccga tcacggaccg cgcggctgtt ggcgaaatgc
     3241 tgagcggcct atccggcaac cttgatgtga tcgtgcctcg cggcggtaaa gatctcgtgg
     3301 cccgtgtcca ggccgaggca agagtgccgg ttttcgctca tcttgaaggc attgtgcatg
     3361 tgttcgtcga ccgtgacgca gacctgaaaa aagcaaaggc aattttattt aacgcgaagc
     3421 tccgccgcac gggaatttgc ggcgcggccg agactcttct tgtcgaccgc gtctgcgcgc
     3481 cgacgcatct tgcgccgctt gtcaaaatgc tgcttgatgc cggctgcaca gtgcgcggag
     3541 atggaacaac ctgcgccgca gacccgcgtg tcacgccggc aactgaggcg gattggtcga
     3601 ccgaatatct cgatgcgatc atttccgtgc gggttgttga tgacctcgat gaggccattg
     3661 cgcatattga aacttttggt tcgcgtcata ccgattgcat cgtcacagaa aataaagaga
     3721 gggcggggcg tttcttgagg gaagtggatt cggccattgt aatgcacaat gcgtcaacgc
     3781 aattcgccga tggcggcgaa tttggatttg gggccgagat tggcattgca acgggccgga
     3841 tgcacgcgcg gggacccgtc gggctagagc agctgacgtc attcaaatac cgcgtgcacg
     3901 gcgcgggaca aactcgggcc tgagggtgta ctggttgtcg aagtctggat ttgacttgac
     3961 tgatacttcc ctatgaaatc gaagttagcc attcatctct tggcgacgag tttggcgagc
     4021 ggatcgattc tgtcaaggac gcgagcgaat gctcgcgccg gcgcagccgg tgctccgcat
     4081 ccttgacaga ctcgagtccg tctcgccgat catgctcgcc gtgcgatgaa aggcgaaatc
     4141 tgactttcgt caaaggttcc gaaacccaac ccgaaaagag atacttttgg agcctcctgc
     4201 tgtctcggcc gggtcggtcc cggccttcca aacgccactg tcggaattgt ggggagcccg
     4261 tcgcactgca gcgtcgcgta accttccacc atttgtgcca ggcttgaggg tcggcttgtt
     4321 tggcggcagt ttcaatccgc cgcacgatgg ccatagggcg gcgagccttc ttgccttgcg
     4381 gcggctcagc ctcgatcgcg tgtggtggct cgtcacgccc aacaaccctc taaaggacgc
     4441 acgggagttg gcgcctctcg cgctgcggct tgaggcggca cgaaaagtga gccgccatcc
     4501 gaggatcgaa gtcacggcga tcgaagcggc aatcggcgca acttatagtt ttgagacgat
     4561 ttcttatctg aaaaggcgct gccccagcgt ccatttcgtc tggttaatgg gcgcggataa
     4621 ttttcagtac ttcgatcgct ggcagcgatg gcgcgagatc gcagagcttg ttcctatcgc
     4681 tatcatcgat cggcctggtt tcacgttaac agccacacac gggagagccg cagtaacatt
     4741 ggcgcgtaac cgtctaaatg aaacgcgcgc ctccgttctg gcgactgtta tgccccccgc
     4801 tttggtgttt ctgcatgggc cgcgttcacc aaaatcctcg acggagttac gccgcaaact
     4861 acattgtgag agcaaagaaa ggtagcgtgt tagtcctgcg catgacccaa tgtatagacc
     4921 ggctgcattt cgcaactgag gacgtgctgg atagcggaac tcgctgatct gtatccggcc
     4981 tactggtcgg ctcgcgggcc gtggccttga tgggaatacg cacgcgcctt tggtcttatt
     5041 agcggacagg ttgtcgggca accatttggg tcatcagact ctgggcacgt tcattgatcc
     5101 gttccatgca gtgcatcaat cgcgaactat ttgaggtttc ggctcagatg gccgcaacct
     5161 cgtgacggag gacgtcgaat accttgccgt ttcgcaggac actccatgcg atcctggcga
     5221 gcttgttt
//
LOCUS       DATMPU010000232         5932 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_2987824,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000232 DATMPU010000000
VERSION     DATMPU010000232.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 5932)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5932)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5932
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2987824"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(12..1163)
                     /gene="hpnH"
                     /locus_tag="VNY06_05310"
     CDS             complement(12..1163)
                     /gene="hpnH"
                     /locus_tag="VNY06_05310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012386067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosyl-hopene transferase HpnH"
                     /protein_id="HWX14261.1"
                     /translation="MGIPVLQMVQIGAYVVRQRLAGRKRFPLVLMLEPLFRCNLACAG
                     CGKIDYPDKILNQRLPLADCLSAIDECGAPVVVIAGGEPLLHKELPEIVKGAIARRKF
                     VTVCTNALLLAKNIDRYTPSRYFNWSIHLDGDAEMHDLSVCQAGVYERAIEALKLAKT
                     RGFRVTINCTLFNNAEPERVAAFFDRVAALGIEGITVSPGYAYERAPDQQHFLNREKT
                     KTLFRQIFALGRSGKAWPLFQSQLFLDFLAGNRTYACTPWGNPTRTIFGWQRPCYLLG
                     EGYTATFTELMEETDWAAYGTGNYEKCADCMVHSGYEATAVTEAVTKPWKMLGLATKG
                     IRTTGPMAPDISLENQRPAEYVFSRHVQQAMEKIKHQKPTAEDLVDAAD"
     gene            complement(1212..2135)
                     /gene="ispH"
                     /locus_tag="VNY06_05315"
                     /gene_synonym="lytB"
     CDS             complement(1212..2135)
                     /gene="ispH"
                     /locus_tag="VNY06_05315"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002711127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA];
                     GO_process: GO:0050992 - dimethylallyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HWX14262.1"
                     /translation="MKVILAQPRGFCAGVVRAIYIVERALEKYGPPVYVRHEIVHNGY
                     VVESLKAKGARFVERLDEVPPGARTIFSAHGVARSVEDEAAALGLPVLDATCPLVSKV
                     HSQGQRHVQHGRTLVLIGHAGHPEVEGTMGRIPGQIHLVQTEADVAKLDLPPNTAVAY
                     ISQTTLSVDDTKAIIAALKRRFFDIVGPETRDICYATQNRQSAVRELSRLVDVLLVVG
                     AKNSSNSNRLREIGAEASIPAFLIADRSELDFSALRDAETIGLTAGASAPEILVEEVL
                     EALAALGPIELETLPGVAENIEFKLPAELGD"
     gene            2449..5076
                     /locus_tag="VNY06_05320"
     CDS             2449..5076
                     /locus_tag="VNY06_05320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012592222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HWX14263.1"
                     /translation="MNPVAPDPIARSIIAIVAFCSRFRWAVLASSILLTIAAANYALT
                     HFAINTNTNNFISEKLQWRQNLIALDKAFPQRVDQIVIVIDGATAELAEAAAQALTDK
                     LKGRPDLYRSVVRPEGGAYFNETGLLFLPVSELRKTVSELSRARAFLMALSSDPSLRG
                     IVTALSYINQGVRAQAGTLDDFDRPMVQFSDELEGVLAGRPEYFSWRSLISGQPPDPR
                     ELRRFIEIEPVLDYAALMPGEKASDFIRETARNLGFSPSHGVAVRLTGLVPMADEEFS
                     TLADGAELNGIVTALMVLFIIWLALKSGRIVLAVAFTVIVGLALTAAVGLMMVGALNL
                     ISVAFAVLFVGIGVDFGIQFSVRYRAERFKEPRFDKALVQAAGKAGRPLALAAAATAA
                     GFYSFLPTDYRGVSELGLIAGTGMFIAFFAAITVLPALLSLFRPPPEQEPVGYLFLAP
                     VDRFMADHRYAIVSITLLVALAGTPLLRHLRFDFNPLNLRSSKVESVATLLDLMNDPS
                     TDTNTVDILAPNLSAIPPLVARLEKLPEVARVTSIYSLVPEDQDQKIAIVAPLAKSLL
                     PLLDPAREREPPTDAQTVEAMERAANAFTATANGQTGKGADDARRLADLLRQMAAAPP
                     QLREEARKALVPGLDMMLDLLRTSLTAKKVTIASIPPDILRNWVTPDGRARIQVAPSG
                     DPNSNDNLLRFTAAVRAIDPTAMGEPIAVQESGNTVVRAFIEAGLWALISVMLLLFIV
                     LRRITDVLLTLVPLMLAGLVTLEITVLIGLPLNFANIIALPLLLGLGVAFKIYFVMAW
                     RAGTTNLLQSSLTRAVFFSAMTTATAFGSLWLSKHPGTSSMGKLLALSLGCTLAAAVL
                     FQPALMGPPRKVDPTGEDA"
     gene            complement(5102..>5932)
                     /locus_tag="VNY06_05325"
     CDS             complement(5102..>5932)
                     /locus_tag="VNY06_05325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011087849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HWX14264.1"
                     /translation="GPIARTVRDAAILLRSMAGHDPKDTTSADLPVPDFEAAVGRGVK
                     GMKIGIPKEYRRDGMAGEIEALWAQGIAWLKEAGAVIADISLPHTRYALPAYYIVAPA
                     EASSNLARYDGVRYGLRVPGKNIVDMYEKTRAAGFGKEVRRRIMIGTYVLSAGYYDAY
                     YLRAQKVRTLIKQDFEKAFGEGIDAILTPATPSAAFGLGEKGSADPVEMYLNDIFTVT
                     VNMAGLPGIAVPAGLSAEGLPLGLQVIGRAFDEETLFSVAEVFEQSAPKIAPPEKWWT
                     "
BASE COUNT         1174 a         1808 c         1645 g         1305 t
ORIGIN      
        1 tcgatgctgc tctagtccgc ggcgtcgacg aggtcctcag ccgtaggctt ctggtgtttg
       61 atcttctcca tcgcctgctg cacgtgccgg ctgaacacgt attcggcagg gcgctggttc
      121 tccagcgaga tatcgggcgc catcggcccg gtggtgcgga tgcccttggt ggcgaggccg
      181 agcatcttcc acggcttggt gacggcttcg gtgacggcgg tggcctcgta gccgctatgg
      241 accatgcaat cggcgcattt ttcgtaattg ccagtgccgt aagcggccca atcggtttcc
      301 tccatcaact ccgtaaaagt ggccgtgtag ccttcgccga gtaaataaca gggccgttgc
      361 cagccaaaga ttgtgcgcgt cgggttgccc catggcgtgc acgcataggt ccgattgccg
      421 gcaagaaaat cgaggaaaag ttgggactgg aagaggggcc aggccttacc gctgcggccg
      481 agcgcaaaga tctgccggaa aagagtcttg gttttctcgc ggttgaggaa atgctgctgg
      541 tcgggcgcgc gctcataggc atagccaggc gacacggtga taccctcgat gccgagcgcc
      601 gcaacacgat cgaaaaaagc tgcaacccgt tcaggttcgg cattgttgaa aagcgtgcaa
      661 ttgatggtca cccgaaatcc acgcgtcttc gccagtttta gagcctcaat cgcgcgttcg
      721 taaacaccag cctgacagac cgaaagatca tgcatctcgg cgtcgccatc gagatggatc
      781 gaccagttga agtagcggct cggcgtatac cggtcgatat tcttcgccag cagaagcgcg
      841 ttggtacaga cggtcacgaa tttgcgccgg gcgatggcac ccttcactat ctctggcagc
      901 tccttgtgga gcagaggttc gccacccgcg atgaccacga cgggcgcacc gcattcgtca
      961 atcgccgaaa gacaatcggc aagcggcagg cgctgattca aaatcttgtc gggatagtcg
     1021 atttttccgc aacccgcaca agccagattg cagcggaaaa ggggttcgag catcaagacg
     1081 agaggaaaac gcttgcgtcc agccaggcgc tggcggacaa cataagcgcc gatctgaacc
     1141 atttgcagca ccggtattcc caaagccttc ctcccaaata ggtgtcaccc gcgggcatcg
     1201 ccccgggaaa gctaatcacc gagttctgca ggaagtttaa actctatatt ttccgcgacc
     1261 ccaggcaggg tctccaattc gattgggcca agtgccgcca aggcctccag cacctcctcg
     1321 accagaattt ccggcgcaga ggcgccagcg gtgaggccaa tcgtttcggc atcgcgcaag
     1381 gcggaaaaat caagttcaga cctatccgca attaagaacg caggaatgct tgcctcggcg
     1441 ccgatttccc gcagacggtt tgaattcgag ctgttcttgg cgccaaccac gagcaacacg
     1501 tcaacaagcc tgcttaattc acgaaccgca ctctggcggt tctgtgtggc gtaacaaata
     1561 tcccttgtct ccggtccgac gatgtcgaag aaccggcgct tcaatgcggc gataatcgcc
     1621 ttggtatcat cgacactcaa ggtcgtctgc gagatgtaag ccaccgccgt atttggcggc
     1681 aagtcgagtt tagccacatc agcttcggtt tgcacgagat ggatctgccc tggaatgcgt
     1741 cccatggttc cttccacctc tggatggccg gcgtggccaa tcagcacaag ggtcctcccg
     1801 tgttgcacat ggcgctgccc ttggctatga accttcgata ccaggggaca tgtggcgtca
     1861 agaaccggca gtcccagtgc ggcggcttcg tcctcgactg agcgggcgac gccatgggcg
     1921 ctaaaaattg tgcgcgcgcc gggaggcacc tcgtcgagcc gctcgacaaa gcgggcccct
     1981 tttgccttga ggctttcaac gacatagcca ttatgaacaa tctcgtggcg aacataaacg
     2041 ggcggcccgt atttttcgag cgcacgttcc acaatgtaaa tcgcgcggac aactccggca
     2101 caaaatccgc gcggttgagc taagatgact ttcatgaaat ttacctggcg ggagatcgga
     2161 tgtagttgaa aaacgaaagc cttctgggta cggcgcgtcc ctgctgccaa gcgtgacttg
     2221 tagccctctc gataccaatg tcaaacagct gacactaaca cttcacacga ttgacattgc
     2281 cggtctctcg gccaatgtct gttgaacctc tgtcagacgc gacagcaatc ctggctcacc
     2341 gggccttttt gccgaggatt gtttttttcg ttacccttgc tgccgtcaaa ccggcagacg
     2401 accgcagggc aaggtggaat ccataaaacc cgggtgagat cgctatttat gaatcccgtc
     2461 gctcccgacc ccattgcaag atcgatcata gccatcgtgg ccttttgcag ccgtttccgt
     2521 tgggccgtcc ttgcctcgtc aatcctattg acaatcgccg ccgctaatta tgccctgacc
     2581 catttcgcta taaacaccaa tacaaacaac tttatttcgg aaaagttgca atggcggcag
     2641 aatcttattg ctctggacaa ggcgtttccg cagcgtgtcg atcaaatcgt catcgtcatc
     2701 gatggcgcga cagccgagct cgccgaggcg gcggcgcagg cgctgaccga taagctaaag
     2761 ggccggcctg atctttatcg atcggtggtc cggcctgaag ggggagctta ttttaatgag
     2821 accgggctcc tgtttctccc tgtctccgaa cttcgcaaga cggtcagcga gctctcccgc
     2881 gcccgcgcat ttctgatggc gctttcctcc gatccgagtt tgcgcggaat cgtaaccgcc
     2941 ctgtcctaca ttaatcaagg agttcgggcg caagctggaa cgcttgacga tttcgaccgg
     3001 ccgatggtgc agttcagcga tgagctggaa ggcgtgctcg caggccggcc ggagtatttt
     3061 tcgtggcgca gcctcatctc tgggcaaccg ccagatccgc gggaattgcg ccgattcatc
     3121 gagatcgagc cagtccttga ttatgcggcc ttgatgccag gggagaaggc gtcggatttt
     3181 atccgcgaaa ccgcgcgcaa tcttggtttt tcgccgagcc acggcgtagc ggtcagattg
     3241 accggcctcg tgccaatggc cgatgaggaa ttcagcactc ttgccgatgg cgccgagctc
     3301 aacggcattg tcacagcgct catggtcctt tttatcattt ggctggcctt gaagtcgggc
     3361 cgtattgtgc tcgctgtcgc cttcaccgtt attgtcggtc tcgctctcac tgccgccgtt
     3421 ggccttatga tggtcggcgc actcaatttg atttcggtcg cctttgccgt gctgttcgtg
     3481 ggcattggcg ttgatttcgg gattcagttc agcgttcgct accgcgccga acgctttaaa
     3541 gagccgaggt tcgacaaggc cctcgttcaa gcggcgggca aggcggggcg gcccttggct
     3601 ctcgccgcgg ccgcaaccgc cgcgggtttt tattcgtttt tgccaaccga ctatcgcggc
     3661 gtttcggaat tgggcctcat cgccggaacc ggcatgttca tcgccttttt cgccgcgatc
     3721 acggtcctcc cggcattgct ttccctgttc cgtccaccgc cggaacagga gcctgttggc
     3781 tatctctttc tcgcgcccgt ggaccgattc atggccgatc accgttatgc gatcgtctcg
     3841 ataacgctgc tggtcgctct agcagggaca cctttgctga ggcaccttcg tttcgatttt
     3901 aatccactca acttgcgcag cagcaaggtc gaatctgttg ccactctcct ggacttaatg
     3961 aacgatccaa gcacggacac caacaccgta gatatcctcg caccaaacct ttctgccatc
     4021 ccgcctctcg tcgcacgtct cgaaaaatta ccagaggtcg cccgggtcac gagcatttat
     4081 agcctcgtgc ctgaagatca ggaccaaaag atcgcgattg tcgcgcctct tgccaagtct
     4141 ttgctgcccc tgctcgatcc tgcccgcgag cgcgagccgc ccaccgatgc acaaaccgtt
     4201 gaagccatgg agcgcgcggc aaatgctttc accgcgacgg cgaatggtca gaccggcaag
     4261 ggcgccgacg acgcacgccg cctcgctgac ctgctgcgcc aaatggctgc cgcgccgcca
     4321 cagctgcgcg aggaggcacg aaaggccctg gtgccaggcc tcgatatgat gctcgatctg
     4381 ctgcgcacat cgttaactgc gaaaaaggtg acgattgctt caattccccc cgatatcttg
     4441 cgcaattggg tgacgcccga tggccgcgcc cgtatccagg tcgcgccttc cggcgatccg
     4501 aacagcaatg acaatctcct ccgcttcacg gcggcggttc gcgccatcga cccaactgcc
     4561 atgggtgagc ccatcgcggt tcaagaatct ggcaataccg ttgtccgggc atttattgaa
     4621 gccgggctct gggcgctgat ttcggtcatg ctgcttctct tcatcgtgct gcggcggatt
     4681 accgatgtgc tgctgacctt ggttcctttg atgctcgctg gcctcgtcac gcttgaaatc
     4741 acggttctga tcgggctgcc gctgaatttt gccaatatta tcgccctccc gcttttgctt
     4801 gggcttggcg ttgctttcaa gatttatttc gtcatggcat ggcgggcggg gaccacaaat
     4861 cttctgcaat caagtctcac tcgcgcagtc tttttcagcg cgatgacaac cgcgactgct
     4921 tttggaagtc tctggctctc caaacatccc ggcacctcca gcatgggcaa gctgctggcc
     4981 ttgtcgcttg ggtgcacact ggcggcggcg gtgctgttcc agcctgcatt gatggggccg
     5041 ccgcggaagg tcgacccaac aggcgaagat gcctgatccg cgcggcccct ttgcagccaa
     5101 actaggtcca ccatttctcc ggcggggcga tttttggcgc cgattgctca aaaacctctg
     5161 ccacggagaa cagtgtctct tcgtcgaatg cgcggccgat cacttgcagc cctaaaggca
     5221 agccctccgc cgaaaggcct gccggcacgg ctatgcccgg caggcccgcc atgttcaccg
     5281 tgacggtaaa aatatcgttg agatacattt caaccggatc cgccgagccc ttttcgccaa
     5341 gcccaaaggc cgccgatggc gttgccgggg tcaggatcgc gtcgatcccc tccccaaacg
     5401 ctttctcaaa atcctgcttg atcagggtcc taaccttttg cgctcttaag tagtaagcat
     5461 cataatagcc ggccgagagc acataggtac cgatcatgat gcgccggcgc acctctttgc
     5521 cgaaacccgc ggcgcgggtt ttttcataca tgtcgacgat attcttgccc ggcacgcgaa
     5581 gcccatagcg cacgccatca tagcgcgcga gattggaaga ggcttccgcc ggcgccacga
     5641 tataataggc aggcagagca tagcgcgtat gcggcagcga gatgtcggca ataaccgcgc
     5701 ccgcctcttt cagccaggcg atgccctgcg cccacagcgc ttcgatctcg cctgccatgc
     5761 catcgcggcg atattctttc ggaatgccga tcttcatccc cttgacgccg cgccccacgg
     5821 ccgcctcaaa atccggcacc ggaagatcgg cggaagttgt gtccttcggg tcatggcccg
     5881 ccatggagcg cagcaaaatc gccgcgtcgc gcactgtgcg ggcaataggc cc
//
LOCUS       DATMPU010000233         2976 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_2989294,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000233 DATMPU010000000
VERSION     DATMPU010000233.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 2976)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2976)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2976
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2989294"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1136
                     /locus_tag="VNY06_05330"
     CDS             <1..1136
                     /locus_tag="VNY06_05330"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HWX14265.1"
                     /translation="SYIAAGGPILLAAAVDPGWSKALWVGGLFLVTEPLMGQLVEPML
                     YGRSTGLSPISVVLSAIFWGWLWGPVGLIISTPLTLCFVVLGRHVEQLELLNILLGDR
                     PALTKIENFYQRALAGDVDEVQEHADELLEEIDLGSYYDEVAMPGLELAARDIARGVL
                     TRAQMERIKETVTALVAELEYHADEKPATNVEKRPITPEVGKELRRSVENPETPTPPS
                     EQSKAQLRCIAARTPLDEASATMLAQLLRSQGLAAEVVSKETVSRTGMGRFESDCVAA
                     VCICFVDASGSTSALRFLVRRLRQRLPSAHLLIIAWPKDHPLLSDKRLKENLGEAGYA
                     SSLRDAVRFCLTVIVEYSDSTNTPNPEMEIAQTGGDTTPAVSR"
     gene            1148..2497
                     /locus_tag="VNY06_05335"
     CDS             1148..2497
                     /locus_tag="VNY06_05335"
                     /inference="COORDINATES: protein motif:HMM:NF024241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWX14266.1"
                     /translation="MRHNPPFLRMIASRLGIAKQSVAQKTMPALRRVPSILAHYRDAR
                     KLLRGSISKQSRRGLDWLNFFLADVQTGFGAFVAFYLAELGWSKAQVGLALAAGTIAA
                     LIAQIPGGALVDAAPWKRGLAGIGICMIGVSALILAVAPAHVLVFVAQVLNGVSGAIV
                     TPAIAAISLGLVGRSAMSARTGRNTRFNALGNVLTAGAMGLAGQYLAKSAIFFGAAVL
                     CVPALIALIRIRGDEIDYARARNAGIGKQAGNIHRIFDIGKNAKLYIFAGCLFMFQFA
                     DASMLPVIGQEVARYQSGPSAVLMAGLIAVPQVITALISPWVGYHSERFGRKPLLLMG
                     FGIEIFRALLFASFSNYWILIIAQLLSGASAAAVTVLTILVITDLTTGTGRFNLVRGF
                     VGTVIAIAASISTGITGFIFQDLGNWEGFLILAGFATAAAALLWFAMPETRPTKYLD"
BASE COUNT          607 a          792 c          864 g          713 t
ORIGIN      
        1 gcagttatat tgctgccggt gggcctattt tgctggccgc ggctgtagat cccggatggt
       61 caaaggctct gtgggtgggc gggctctttc tggtgactga gcccctgatg ggacaattgg
      121 ttgagccgat gctgtatggc cgcagcacag gcttgtcgcc aatctccgtc gtactttccg
      181 cgatcttttg gggctggctg tggggtccgg tcgggttgat tatttcgacc ccgctaaccc
      241 tatgcttcgt agtgctcggc cgacatgtcg aacaactcga attgcttaac atcctccttg
      301 gagatcgtcc agctttgacg aagatagaga atttttatca acgtgcgctt gctggagacg
      361 tcgacgaagt tcaggaacac gctgacgagt tgctggaaga gatcgatctc ggatcctact
      421 acgatgaggt cgccatgcca ggtctggaat tggccgcgcg cgatattgcg cgcggtgttc
      481 ttacccgtgc acaaatggaa cgcattaaag aaacagtgac agcgcttgta gcagagctgg
      541 aatatcatgc ggacgaaaag cccgcgacta acgtggagaa acgccccata accccagagg
      601 tcggcaaaga attgcgaagg tctgtagaga accccgagac gccaacgccg ccgagcgagc
      661 aaagtaaagc gcagctccgc tgtatcgccg cccgcactcc gcttgacgag gcgtctgcca
      721 caatgctcgc tcagcttcta cgaagccagg gtttggctgc ggaagtagtc tcgaaggaaa
      781 ccgtatcgcg aacgggaatg ggtcgctttg aaagcgactg cgtagccgca gtctgtatct
      841 gctttgtcga cgcgagcgga tcgacctcgg cattgcggtt tctagtgagg aggttgcgac
      901 agcgcttgcc cagtgcgcac cttctcatca tcgcctggcc taaggaccat cctttgttgt
      961 ccgacaagcg attgaaggaa aacttggggg aagctggata tgcatcttcg ctgcgcgacg
     1021 cggtaagatt ttgcttgacc gttatcgtag aatattcgga ctccaccaat acgccaaacc
     1081 cggaaatgga aatcgctcag accggaggtg acaccacccc cgcagtctcc cgctgatcgg
     1141 aaaggcgatg cgacacaatc cccctttttt gagaatgatc gcgagtagac taggtattgc
     1201 aaaacaatct gttgcccaaa aaactatgcc ggctttgcgg cgcgttcctt ctatccttgc
     1261 gcactatcga gatgcccgca aactgctgcg cggctcgatc tcaaagcaaa gtcgacgagg
     1321 actcgactgg ctgaatttct ttctggccga tgttcagacg gggtttggtg ccttcgtagc
     1381 attttatctt gctgaattgg ggtggtcaaa ggcgcaagtc ggcctggcgc tcgctgccgg
     1441 tacgatcgct gccttgattg ctcaaattcc cggtggcgcg ctcgtagatg ctgcgccgtg
     1501 gaaacgaggc cttgccggta tcggcatttg catgatcgga gtatctgctc tgatccttgc
     1561 ggtggcgccg gctcatgtgc tcgtattcgt cgctcaggtc ctaaacggag tgagcggcgc
     1621 catcgtcacc ccggcgattg ccgcaataag cctcggattg gtcggccgca gcgcaatgtc
     1681 cgcacggaca ggacgcaaca ctcgcttcaa tgcgcttggc aacgtgctta cagcaggggc
     1741 gatgggcttg gcgggtcagt atttggccaa aagcgcaata ttctttggcg ccgccgtgct
     1801 ctgtgtccct gcccttatcg cgctcattcg cattcgcggc gacgaaatcg attatgcgcg
     1861 cgcccgcaat gcaggaattg gcaagcaggc aggcaacatt caccgtattt tcgatatcgg
     1921 caaaaacgca aagctttaca tctttgcagg gtgcttgttt atgttccagt tcgcggacgc
     1981 ctcgatgttg ccggtgatcg gccaggaggt cgcgcggtat caaagcggac cgtccgcggt
     2041 tttgatggcg ggcctgatcg ccgtgccgca ggttattacc gcattaatct cgccttgggt
     2101 gggctaccac tctgaaaggt tcggacgcaa gccacttcta ctaatgggct ttggaataga
     2161 aatttttcga gctctgttgt ttgcatcctt ctccaactat tggattctaa tcatcgccca
     2221 gcttctcagc ggcgccagcg ccgcggctgt aacggtgctg acgattcttg taatcaccga
     2281 tctaacaaca ggtaccggtc gtttcaactt agtacgcggt ttcgtcggca ctgtaatcgc
     2341 cattgcggct tctatcagta ccggaattac cggcttcatt tttcaagatc tgggaaattg
     2401 ggagggtttt ctgatcctgg cgggttttgc cactgcggct gccgcactct tgtggtttgc
     2461 gatgccggag acacggccga ctaaatatct tgactgaggc agtggcgttc tgcctgcagg
     2521 agccgtgccg tctaggacgg gactctagcg gagcgattgc ctcgacgtcg gaccacggtc
     2581 aaagcgcgca cgatggttat aacccgcgaa gtcctctttc tgtaggtggt tttcgtgacg
     2641 tctaaggtgg ttgttgagtg gggtgctggc agcggcacgg tgcagcggct aaatgctcgg
     2701 ccacaagcgt atcagcgcaa tcgtccattt cacggccggg gacccatttc ggcgatgtcg
     2761 gcgtcgcgtt cgccagcaga gcaacgtgcc aagctacgat gggagcgtgt cattacatgc
     2821 gatcaaggct gccctcatca ataaaacagg cgatggcaat taagcatgct tgcaaattgt
     2881 tggaacctgg ggccgtgaac cgcattccct tggcgggttt gggttggctt gaggaagtca
     2941 tcatgatcgg aaccatactg atcattattc tcatag
//
LOCUS       DATMPU010000234         2243 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_3600382,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000234 DATMPU010000000
VERSION     DATMPU010000234.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 2243)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2243)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2243
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3600382"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..351
                     /locus_tag="VNY06_05340"
     CDS             <1..351
                     /locus_tag="VNY06_05340"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HWX14267.1"
                     /translation="ITSPIAIFEAALGICRKRHASVEEAEQDIGEFLELAGVRTAPIT
                     DKKAHTALAAFSRYGKGRGHPAQLNLGDCFAYAMAKNARTPLLFKGEDFDRTDIRRAA
                     QLLKIAASRLPRDV"
     gene            749..949
                     /locus_tag="VNY06_05345"
                     /pseudo
     CDS             749..949
                     /locus_tag="VNY06_05345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558360.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
     gene            999..1364
                     /locus_tag="VNY06_05350"
     CDS             999..1364
                     /locus_tag="VNY06_05350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX14268.1"
                     /translation="MTDARPLSDQACSAGFGFVSHLVLSLLPALAGCASADQGCVPTM
                     AELRIGITKADAVNAWCYPSQAAKTESAVTVFDPASRSTKPGTMVRETWIYTLGPLAG
                     SFLAFDENGRLVAVSEAHH"
BASE COUNT          508 a          645 c          620 g          470 t
ORIGIN      
        1 atcacctcgc cgatcgccat cttcgaggcg gccctgggga tctgccgaaa acggcacgcg
       61 agcgtggagg aggctgagca ggacataggc gaatttctag agttggcggg agtgcggacg
      121 gccccgatca ccgacaagaa ggcgcatacg gcgctcgcgg cgttctctcg ctacggcaag
      181 ggacgggggc atcctgcgca actcaacctc ggcgactgct ttgcttacgc catggcaaag
      241 aacgctcgga cgccgctgct cttcaagggc gaggatttcg acaggaccga catccgccgc
      301 gccgcgcaat tgctaaaaat cgcggcgtcg cggctgcccc gcgatgtgtg agatggcggc
      361 ctctatcgta ccctcagcat tccggtggtc gagcgcctcg gatttccaac gtagcgccta
      421 cgccaagcgg tcgagcttgc tcccagttcg tttcgatcta caacagatcg gcggcagccg
      481 cgcgcaacgt gacattgtgg actggaccct gacggagccc ctatcgcgcc ggtcagcggg
      541 cgtgccttgt cgcctgcgca tgagcgtctg aggacgagtt cgcgctgcgc cgcccaaacc
      601 ggcgcttttt tggcaaagat cgcgtaaact gtcgccgtca cctgaaaccg gtaaatcgag
      661 gtctgcaccg gatagacgct ggatatttct tcgcagcctc gtctttgctg tcgtgccaaa
      721 ccaaatacgg taatttttct ctactgcaat ttggatcgcc gtctcggtct acgtcctcgt
      781 cgcaatcgtc aagaagcgcc tcaccctcga cgcctcgctc tacacattgc tccagattct
      841 ctcggtcacc ctcttcgaga aaatcccgat agatcaagtg cttgcccgag acgatcccaa
      901 agaccatact tcgcaaataa ctaaccagct gaatctattc gcttattgac cggacagcag
      961 tggtcaagca tatataataa atagagcaac agaaactcat gaccgacgca aggccgctat
     1021 ctgatcaagc atgctcagcg ggctttggct ttgtgtccca ccttgtgctg agcttgttac
     1081 ccgcgcttgc cggctgcgcc agcgccgatc agggctgcgt gccgactatg gccgagctga
     1141 gaattggcat aaccaaagcc gatgcggtca atgcttggtg ctacccatct caggctgcca
     1201 aaaccgagtc ggccgtaacg gtgttcgatc ccgcatcgcg atcgacaaag cccggcacca
     1261 tggtccgcga aacgtggatc tatactcttg gcccccttgc ggggagtttc ctggcgttcg
     1321 acgaaaatgg ccggctcgta gccgtctccg aggcgcatca ttgaccgcga acgtggccgt
     1381 ctgaggggat acaaccttat ttattatcag atgccaagga caaatccgat gaaccgattt
     1441 gatacgaatc gccaggcggg gcgcctagtt atcgatgaat tagccaatcg tggtatacgg
     1501 ttttggaaac aaagaacccg ttactggtta aatcgccttc catcttgagt ttcggttagc
     1561 agtacatgga caacggggga agcgagattg gccgagacac catccgggca tgccgaacat
     1621 cctcgcttac atgccgtgtg ctggggacgc caagtttttc attctttcgg aaggagaagc
     1681 ccagacgtta cacgaggcgt atcatgcgca gtatccaaag aatgaagata agaccggggg
     1741 cgggtttggt cggggtgatc attatccatt cgagggacgc tggtgcacat ttccgaaatg
     1801 agatttcaaa gtgcgtcagt ccccgatttc ccgcttcaat tgacaaaggc gggttctcgt
     1861 ggtatattgt gaatgtatag atggcgtcgg aagcaaattt agcgcgatcc tgacatgaac
     1921 agtagaaaac agccagcaga acaacgatgc ctcgctgttt tcttccccgc caaaccctaa
     1981 gcggcccagg gtttacggcg agaacgaaat accggtggct gataatttgc tgcagataac
     2041 agcaaaaaca gccagaagcg cacgcctcgg gcgacaccac ccgcgggcat tatgtcccct
     2101 gaatgcctct gaacactgaa actcacgttc gagcggcgga cgacacccgg cattgaccgg
     2161 agccggctca ccttcctaca aagaaagagg gcccggctac gaaaggattt gcgcgcggtg
     2221 acggtctttc atctgctgcg gca
//
LOCUS       DATMPU010000235         5065 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_1731700,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000235 DATMPU010000000
VERSION     DATMPU010000235.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 5065)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5065)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5065
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1731700"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..238
                     /locus_tag="VNY06_05355"
     CDS             <1..238
                     /locus_tag="VNY06_05355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008962835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="modification methylase"
                     /protein_id="HWX14269.1"
                     /translation="RAREILVDEQRRYSALVRAEGTVSMGEVTGSIHRMGALAQGLPA
                     CNGWTFWHCERDGKLEPIDQLRNLFRASLSEQAA"
     gene            complement(520..3630)
                     /locus_tag="VNY06_05360"
     CDS             complement(520..3630)
                     /locus_tag="VNY06_05360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011925534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HWX14270.1"
                     /translation="MSFTDIFIRRPVLASVVSFLILLAGIQSAMKLQLRQFPALSSTT
                     ITVTTTYPGANADLIKGFITTPIAQAVASAEGIDTLISNSQQNVSTITLNLLLNANAD
                     RAVADVLAKVSQIKYQLPREAFDPVVTKQTGDSTAVLYMSFNSKNLNPSQITDYLHRV
                     VQPKLQTIDGIASAQILGGQTFAMRVWLNPNRMAARGVTPTDVASALAANNFTAAAGQ
                     IKGDFVQTSINAETSLNSARAFAQLVIAARDDALIRLADIADIELGPESVDSSSAFDG
                     LKAVFIGIYPTPTANPLDVINDVRNAFPELQAQLPAGINASIAYDATKFIRASIWEVE
                     KTLGEAALIVIVVIFLFLGNLRSTIIPIVTIPLSLIGVMTILFALDYSLNLLTLLALV
                     LAIGLVVDDAIVVVENIYRHMEEGMSPRDAALKGAREIASPVISMTITLAAVYAPVGF
                     VSGLTGALFREFAFTLAGSVIVSGIVALTLSPMMCSRLLRPPSVNARGFPALLDRLFD
                     RLRQRYQRRLHKTLNFRALTILILVGILGITGVMFASTPKELAPEEDQGVVFIVAKTP
                     QYANLDYLEKATRQAEAAATGIPEREHVFNINGSAGMHEGFGGLLLTPWGERKRGQKQ
                     IMEALQPKLATVTGAEILAFGPPSLPGSTGGPPVQFVIRTIADYRQLVDVMAAIETAA
                     RESGLFLFANSDLKFDLPQINFKIDADKANRLGISMRDVGTSLATLLGGNYVNRFSLQ
                     GRSYQVIPQAPREFRLTPDWLTRYQLRTSAGALVSLATIASVSTNVQPNGLPTFQQLN
                     SATLQGVPFPGHTLGEAISFLQQKAKEVLPEGYSIDYQGESRQFVKEGNSLIATFVFA
                     LVVIFLVLAAQFESFRDPFIILIALPTSMFGALLPLNLLGVVGAASMNIYSQIGLVTL
                     IGLISKHGILMVEFANKLQDQEGLGRREAIEHAAAVRLRPILMTTAAMVVGMVPLIIA
                     EGAGANSRFTIGIVIAAGMSIGTLFTLFVTPAIYTLIARDHGGKRGEQLSDLKRDYSP
                     KRWAAE"
     gene            complement(3634..4791)
                     /locus_tag="VNY06_05365"
     CDS             complement(3634..4791)
                     /locus_tag="VNY06_05365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012589983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HWX14271.1"
                     /translation="MKRTIVFIAVLTFLVTLGGGLGYFQFVLKPNMIKQFILKAPPPP
                     ATVAVTTAASETWANRLPAIGTFRAFQEINIAPQIGGVIVAVRVESGQDVAKGDLLFE
                     IDNSVEQADLKNNLAVLKNAELALERQRRLIQRGNTAEAKVDSAEAERDSAAAAVERV
                     RAIITQKMLTAPFSGRLGIRKLDLGQYVSPGTSLITLQQLDPIFVDFPIPEKSLDLLK
                     PGQVIEVEVDAYPGQIFRGAVKTIDARVANDSRNVWVRGQFDNNKLQLLPGMFANVKV
                     IAGQDQKVVTLPRTAVTYSLYGESVFVVTPDAHDPTEAQAAPGGADRVMKIERKPVRT
                     GATVEDRIAITEGVDPGTIVVSEGQLKLQSGARVRIDPSARLEPASARPKE"
BASE COUNT         1166 a         1386 c         1414 g         1099 t
ORIGIN      
        1 ccgagcaaga gaaattctgg ttgatgaaca gagacggtac tccgcactag tgcgggccga
       61 aggcacggtt tccatgggtg aggtgaccgg atcaattcac agaatgggtg cgctggcgca
      121 aggccttccc gcttgcaacg gctggacgtt ctggcattgc gaacgtgacg gtaaactgga
      181 accgatcgac cagctgcgga atttattccg cgcaagcctg agcgagcaag ccgcgtgata
      241 ccatttcccc tgcgaggttt ggggcgattg gttttggcta gccatggaaa tttgtttggc
      301 tcatagttcg ctcgtgctcc ccgtccgagg cgcagcgcct ggatcggcta ccggcatgga
      361 aatattctcc gcaaggcgat ttgattgaaa aaatcttgca ctgtgtaggc ttgcggcgaa
      421 ttttgagtct ttgcttccaa acggcgggca acgaagactg ggccggcagc gcctttgcca
      481 aacctataat tgcaagtaaa tgcgtctgga aatcctaatt tactcagctg cccaccgctt
      541 tgggctatag tctcgtttta ggtccgaaag ctgttctccg cgcttcccgc cgtgatcgcg
      601 cgcgatgagc gtgtaaatcg ccggcgtcac gaacagggtg aacagcgtgc cgatgctcat
      661 gccggccgca atgacgattc cgattgtaaa acgactgttc gcgccagctc cctcagcgat
      721 gatgagagga accattccga cgaccatggc cgccgtggtc atgaggattg ggcgaagccg
      781 cacggccgcc gcatgttcga ttgcctcgcg gcgaccgagg ccttcttgat cttgaagctt
      841 gttggcgaat tcgaccatta aaatgccatg cttggaaatg agaccgatta aggtaacgag
      901 gccaatctgc gaatagatat tcatgctggc tgccccaacc actccaagga ggttcaatgg
      961 caggagtgcg ccgaacatcg acgtcggcag ggcaatgaga ataatgaaag gatcgcggaa
     1021 gctttcgaac tgcgccgcaa gcaccaggaa aatcacgaca agcgcgaaga cgaatgtcgc
     1081 gataagcgaa ttgccttctt tcacaaactg acggctttcc ccttggtagt cgatactgta
     1141 accctccggc aagacttcct ttgccttctg ttgcagaaag ctaatggctt ctccaagcgt
     1201 gtgtcctggg aacggcacgc cctgcaaggt cgcagagttg agttgctgga aagttggcaa
     1261 tccgttcggc tgcacgtttg tcgacacgct cgcgatggtc gcaagcgata caagcgcgcc
     1321 cgcgcttgtg cgcagctgat agcgcgtcag ccaatcgggt gttagccgga actcccgcgg
     1381 cgcctgtggg atgacctggt agctgcggcc ttgcaggctg aaacggttaa cgtaattgcc
     1441 tccaagcaac gtcgcaagcg aagtcccgac atcccgcatg ctgattccga gcctgttggc
     1501 tttgtcggca tcgatcttaa agttgatttg cggcaaatca aatttgagat cgctattcgc
     1561 gaagagaaat agcccgcttt cccgggccgc ggtctcgatc gcagccatga cgtccaccag
     1621 ctggcggtag tccgcaatgg tcctgatcac aaattggacg ggcggcccgc ctgtcgatcc
     1681 cggcaacgac ggcggtccaa acgccaagat ctctgctccg gtaacagttg caagtttcgg
     1741 ctgaagagcc tccatgattt gcttctggcc gcgcttgcgc tctccccaag gcgtcagaag
     1801 taggccgcca aacccctcat gcatgccagc cgatccattg atgttgaaca catgctctcg
     1861 ttcgggaatg ccggtggccg ccgcttccgc ctgtcgcgta gccttttcca aatagtcgag
     1921 atttgcgtat tgcggggtct tcgcgacgat aaaaacgacc ccctgatctt cctccggggc
     1981 aagttccttt ggcgttgacg caaacatcac gccggtaatc cccaaaatgc ctacgagtat
     2041 gagaattgtc agagcacgaa agtttagggt tttgtgaagc cgccgctgat agcgctggcg
     2101 gagcctgtca aacagccggt caaggagagc aggaaagccg cgcgcattga ccgacggcgg
     2161 cctgaggagg cgcgaacaca tcatcggcga caaagtaagc gcaactatac ctgatacgat
     2221 gaccgatccc gcgagtgtga aagcgaactc acggaacaat gcgccagtga gaccggagac
     2281 aaacccaact ggagcataga cggcggccag cgtgattgtc atcgaaatca ctggcgaggc
     2341 aatttcgcgc gcgcctttca acgccgcatc gcggggggac attccctctt ccatgtgccg
     2401 gtaaatattc tcgacgacaa caatcgcatc gtctacgacc aggccgattg ccagcacgag
     2461 agcaagcaga gtcaagagat tgagggaata atccagcgca aaaagaatgg tcatcacccc
     2521 gataagggaa agcggaatag tcacgatcgg gataatcgtt gaacgcaaat tgccgaggaa
     2581 cagaaaaatc acaacaatga cgatcagcgc tgcttcgcca agcgtcttct cgacctccca
     2641 gatggaggca cgaatgaatt tcgttgcatc ataggcgatg gaggcattta tcccagcggg
     2701 aagctgcgcc tgaagttctg gaaaagcatt gcgtacatcg ttgatgacat cgagagggtt
     2761 agccgttggg gtcggatata tgccgatgaa gactgccttg aggccgtcaa aggcagagga
     2821 tgaatcgacg gactccgggc caagctcgat atcggctatg tcggcgagcc ttatcagcgc
     2881 gtcgtcgcgc gccgcgatca ccaattgtgc gaaggctcga gccgaattga gggatgtttc
     2941 cgcattaatg ctggtctgaa cgaagtctcc tttgatttgt cccgccgcgg cggtgaaatt
     3001 attggcggcc agcgcggacg caacatccgt tggtgtcaca ccgcgcgcgg ccatccggtt
     3061 cggattgagc cagactcgca ttgcgaaggt ctgaccaccc aatatctgcg cgctcgcaat
     3121 cccatcgatg gtttgcagtt tgggctggac cacgcgatgg aggtaatccg tgatctgcga
     3181 agggtttagg ttctttgagt taaacgacat atagagaacg gcagtcgaat cgcccgtctg
     3241 ctttgtcacg acgggatcga atgcctcccg tggcaattgg tattttattt gactcacctt
     3301 cgccaagaca tccgctacag cgcgatccgc attggcgttg agaagcaggt tgagcgtaat
     3361 cgtcgagaca ttctgctgcg aattggaaat caaagtgtca atgccttccg cgctggctac
     3421 cgcctgcgcg atcggcgtcg tgatgaagcc tttgatcaaa tccgcgttgg cgcctgggta
     3481 ggtagtcgta acggtgattg tcgtcgagga gagcgccgga aactgacgca gctgcagctt
     3541 catagcggac tgaatgccgg cgagcagaat caagaagctg acgacgctgg caaggaccgg
     3601 cctgcggata aagatatctg taaatgacat ccgctattcc ttcggccgcg ccgatgcggg
     3661 ctccagacgt gcggacgggt cgatgcgaac acgcgcaccc gactgcaatt tcagctgacc
     3721 ttcggacacg acaatcgtgc cggggtcaac accctcagta atagcaatcc ggtcttcaac
     3781 ggtggcgccc gtgcgaaccg gctttcgctc aattttcata acgcggtccg caccacctgg
     3841 agccgcctgc gcctcagtgg gatcatgcgc atcgggcgtc acaacaaaaa cactctcgcc
     3901 ataaagagaa taggttaccg ccgtccttgg aagagtcaca accttttgat cctggcccgc
     3961 gatgaccttc acatttgcga acattccagg gagcaattgc agcttgttat tgtcgaactg
     4021 gccacgcacc catacattac ggctatcatt cgcaacacgc gcatcgattg tcttgactgc
     4081 tccacgaaat atttggcctg gataagcatc aacctcgact tcgatgacct gaccaggctt
     4141 tagcaaatcg agcgatttct ctgggatcgg gaaatcaacg aaaatcgggt cgagttgctg
     4201 caaagtaatc agacttgttc cgggcgaaac atattgtccg aggtccagtt tacgaatgcc
     4261 taagcgcccg gaaaatggcg ctgtgagcat tttctgggtg ataatggcac gaacccgttc
     4321 gacggcagcg gcagcggaat cgcgctctgc ctcggcggaa tcgactttgg cctcggccgt
     4381 gttgccccgc tggatcaacc gacgctgccg ctccagcgcc aactctgcat tcttgaggac
     4441 cgcgagattg ttcttaagat cagcctgctc gacggaattg tcgatttcaa acagcaggtc
     4501 gcctttcgcg acgtcctgtc ccgattcgac tcggactgct acaatcactc cgcctatttg
     4561 aggagcgata ttgatttcct ggaaggctct aaatgtccca atggcgggaa ggcggttggc
     4621 ccaagtctca gaggctgctg tcgtaaccgc gaccgtggcc ggagggggag gggccttgag
     4681 aatgaattgc tttatcatat tcggctttag aacgaactga aaatacccaa ggccgccgcc
     4741 gagcgtgaca aggaaggtca atacagctat gaaaacgatg gtgcgtttca tatcttcttg
     4801 ccagatgctt gagatgaaat catgctcttt tgcacgccga agctaatcta atggtcaatc
     4861 aatgggctgc aaaaaccggc cctgcggcga gtttggtggg tttcgctatg ctaagcgatc
     4921 cgcccacgcg aagctgcctt ccccaaccat agccgcgatt tccagccatc tcacatattt
     4981 caagaatagc aacttcgatt ttcatcttcg ttcatactgc ggcggtgaat cacacttcgc
     5041 tcctcccgga ccgatggcct gaggc
//
LOCUS       DATMPU010000236         3365 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_1731723,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000236 DATMPU010000000
VERSION     DATMPU010000236.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 3365)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3365)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3365
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1731723"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..802)
                     /locus_tag="VNY06_05370"
     CDS             complement(<1..802)
                     /locus_tag="VNY06_05370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016919956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit beta"
                     /protein_id="HWX14272.1"
                     /translation="MHADIAGLLYVAAGVLFILALRGLASPETARRGNRFGIYGMGIA
                     AGTTFLYQLPWGFSSFFWLLVAVGIGAGIGIWRARTIQMTAMPQLVALFHALIGLAAV
                     LVAASALYAPQGFGLGVRNNIPGSTLFEMALGAAIGAITFTGSVIAFLKLDGRMTGSP
                     ILLNNRHYINIGLGVLLAIFIFAFVPTEAPLVFWLIVLVSFALGVLLIIPIGGADMPV
                     VISMLNSYSGWAAAAIGFTLANLALIITGALVGSSGAILSYIMCKGMNR"
     gene            complement(810..1241)
                     /locus_tag="VNY06_05375"
     CDS             complement(810..1241)
                     /locus_tag="VNY06_05375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003609110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-translocating transhydrogenase family
                     protein"
                     /protein_id="HWX14273.1"
                     /translation="MATSVDPQMLDKIQAATQAAKQAVESAQAYAEQVSAAAHAASGG
                     AIDPFVFRLAVFALAVIVGYYVVWAVTPALHTPLMSVTNAISSVIVVGALLSAGINPD
                     SGLGAGTARFFGFLALTLASVNIFGGFLVTERMLSMFKKKG"
     gene            complement(1303..2442)
                     /locus_tag="VNY06_05380"
     CDS             complement(1303..2442)
                     /locus_tag="VNY06_05380"
                     /EC_number="1.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009763395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008746 - NAD(P)+ transhydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HWX14274.1"
                     /translation="MLIAIPAETDPDEGRVAATPETVKKFVELGAEVRIEAGAGLKSG
                     FLDNEYAQAGGAVVQSAAATLNGADIVLRVRRPATEEIAGVKPGAAVIAIMAPYGHGD
                     ALKSVAGAGVCAFAMEFMPRITRAQSMDVLSSQANVAGYRAVIDAAAEYGRVIPMMMT
                     AAGMIQPAKIFIMGVGVAGLQAIATARKFGAIVTATDVRPATKEQVVSLGAKFIAVED
                     EEFKQAETAAGYAKEMSTGYKAKQAALIAEHISSQDIVITTALIPGRPAPVLITAAMV
                     EAMRPGSVIVDLAVERGGNCELAKAGETVVSKNGAKIIGRLNVAGQVPATASALYAKN
                     LLAFVETMIEPKTKSLAIKWDDELIKATLLTKDGALVHPSFKAGS"
     gene            complement(2540..2689)
                     /locus_tag="VNY06_05385"
     CDS             complement(2540..2689)
                     /locus_tag="VNY06_05385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020173396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aa3-type cytochrome c oxidase subunit IV"
                     /protein_id="HWX14275.1"
                     /translation="MAENEAQPAGHPGMEYDEHEKTYRMFLQLIKYSVAGVAAILALL
                     AFLWG"
     gene            complement(2916..>3365)
                     /locus_tag="VNY06_05390"
     CDS             complement(2916..>3365)
                     /locus_tag="VNY06_05390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019403303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HWX14276.1"
                     /translation="KFVGGVVDLYEGLLHNDFDRTVHAYETWGFRGLTRDLAETLNIW
                     ARFIYAPLLDDRVRSVADGVSPVHYGRKEAFTVHQALKKKGPVLVPREFVFMDRAAIG
                     LGSAFLHLDAKLNFYQLFHETMGKFSDAALRERQARALARAGLAQPA"
BASE COUNT          751 a         1004 c          941 g          669 t
ORIGIN      
        1 agcggttcat ccccttgcac atgatgtagg aaagtatcgc accggatgaa ccgaccaagg
       61 cgccggtgat gatcaaggcg agattggcga gggtgaagcc gatggctgcc gctgcccatc
      121 ccgaatacga attgagcatc gagatcacca caggcatgtc cgcgccgcca atcggaatga
      181 tcagcagaac gccaagcgcg aaggacacga ggacgatcag ccaaaacacc agaggggctt
      241 cggtcggcac gaaagcgaag atgaaaatcg cgagcaaaac accaagaccg atattgatgt
      301 aatggcgatt gttgagaagg attgggctgc ccgtcatcct tccatcaagc ttcaagaaag
      361 caatcaccga cccggtaaag gtgattgcgc caatcgcggc gccaagcgcc atttcgaaaa
      421 gcgttgagcc gggaatgttg tttctgacgc caagcccaaa gccttggggc gcataaagcg
      481 cgctcgccgc gaccagcacc gcggcaagac caatcagggc atgaaacaaa gcgacgagct
      541 ggggcatcgc cgtcatctgg atggtgcggg cgcgccaaat gccgatcccg gcgccaatgc
      601 cgacggcaac cagaagccag aaaaacgacg aaaatcccca aggcagttga tagaggaagg
      661 ttgtccccgc cgctattccc ataccataga tgccgaagcg atttcctctc cgggctgtct
      721 ctggcgaggc caagccacgc aaagcgagga tgaaaagcac ccccgccgca acgtacaaaa
      781 ggccggcgat atctgcgtgc agcatcggct cagccctttt tcttgaacat ggatagcatc
      841 cgttcggtga cgaggaagcc gccaaagatg ttgaccgagg ccagggtgag cgcgaggaag
      901 ccaaagaacc gcgcagtgcc cgccccgagc cccgaatccg gatttatgcc cgccgagaga
      961 agggcgccaa ccacgatgac cgaagaaatc gcattagtga ccgacatcag tggcgtgtgc
     1021 agggcaggtg tcaccgccca gacgacgtaa tagccaacaa tcacagccag cgcgaagacc
     1081 gccagccgga atacaaaagg atcgatcgct ccgccgctcg ccgcatgggc tgcggccgaa
     1141 acttgctcgg cataggcctg cgcgctttcc actgcctgct ttgctgcttg ggttgcggcc
     1201 tgtatcttat ccagcatttg cggatcaaca cttgttgcca tctcctgccc tcctgattgt
     1261 tttttatcga tcgagatcat ggacaatttt gatgtcttgc gctcaacttc ccgccttaaa
     1321 gcttggatgc acaagtgctc cgtcctttgt gagcagggtc gccttgatga gttcgtcgtc
     1381 ccacttgatt gccagcgatt tggttttcgg ttcgatcatc gtctcgacaa aggccaaaag
     1441 atttttggca tagagcgccg aggcggtcgc gggcacctgg cccgcgacat tcaaacggcc
     1501 aataatttta gcgccgtttt ttgacacgac cgtttcgcca gctttcgcca actcgcagtt
     1561 tccgccccgt tccacggcga gatcgacgat aacggagccg ggacgcatcg cttcgaccat
     1621 cgcggcggta atgagaactg gcgccgggcg gccaggaata agagccgtcg tgataacgat
     1681 gtcctgcgag gagatatgtt cagcaatgag tgccgcttgt ttcgctttgt atcccgttga
     1741 catttccttg gcatatccgg ccgctgtttc ggcttgtttg aactcctcat cctcgacagc
     1801 gatgaatttg gcgccgagag acacgacctg ctcttttgtg gcgggcctca cgtctgtcgc
     1861 ggtcacgatg gcgccaaatt tacgcgccgt agcgattgcc tgcagtcccg cgacgcccac
     1921 tcccatgata aagattttcg ccggttgaat catcccggct gccgtcatca tcatcggaat
     1981 aacccggcca tattcggcgg cagcatcgat gacggcgcga taaccggcga cattggcctg
     2041 gcttgagagg acgtccattg actgcgcccg ggtgattcgc ggcataaatt ccattgcaaa
     2101 ggcgcagact ccggcgcccg ccacggattt cagcgcgtcg ccatgaccgt aaggggccat
     2161 gatcgcaatg actgcagcgc ctggctttac cccggcaatc tcctcggttg cgggccgccg
     2221 gacacgcagc acgatgtcgg cgccgttcaa cgttgccgca gcgctctgca cgaccgcgcc
     2281 gcccgcctgc gcatattcat tgtccaaaaa gccagatttc agtccggcgc cggcttcgat
     2341 cctgacctct gccccaagtt ccacgaactt tttcactgtt tccggagtcg ccgcgactcg
     2401 accttcgtcg ggatcggttt cggcaggaat ggcgattagc atgaatagct ccgggaatgc
     2461 ttgaagctgt gacccagccc gattcttgtc cgccggccaa gcctggccac agggaatggc
     2521 tcgctttgta aagggaatat cagccccaga gaaaggccag caaagcgagg atagcggcca
     2581 cgcccgcgac ggaatatttg atcagctgaa ggaacatccg ataggttttt tcgtgctcgt
     2641 catattccat gccgggatgg cccgcgggtt gagcctcgtt ttcagccatt gcgcccacct
     2701 cattttgatc gccggatctg gttcaacctt agcttttgca atgattttcg ccgcgcgcaa
     2761 gagggaaaag cgcggcgcgt gccaaatttt gtgcagagcg gcagccgcct tcgcggaacc
     2821 gtgcttctca aagaactcat gttccatggc aaatcgccgc atggagcttt gagcgccatc
     2881 ggttttggca tcgcagcgat acgcccccgg tctggctaag cgggttgcgc cagtccggcc
     2941 cttgcaagcg cgcgagcctg ccgctcccgc aaggcagcat ccgagaattt gcccatcgtc
     3001 tcatggaaca attggtaaaa attcaacttc gcgtcgagat gcagaaaggc gctgccaagg
     3061 ccgatcgccg cgcgatccat gaagacgaac tcccggggca ccaggacagg gccctttttc
     3121 ttcaaggcct ggtggaccgt aaacgcttcc ttgcgtccgt aatggaccgg cgagacgcca
     3181 tccgcgacgc tgcgcacccg gtcgtccagc aaaggcgcat aaataaagcg cgcccaaata
     3241 ttcaaagttt cggcgagatc cctggtaaga ccgcgaaacc cccaagtttc ataggcgtga
     3301 acggtacggt caaagtcgtt gtgcagtagt ccctcgtaga gatcgacaac accgccaacg
     3361 aattt
//
LOCUS       DATMPU010000237         2793 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_1731731,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000237 DATMPU010000000
VERSION     DATMPU010000237.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 2793)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2793)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2793
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1731731"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..348
                     /locus_tag="VNY06_05395"
     CDS             <1..348
                     /locus_tag="VNY06_05395"
                     /inference="COORDINATES: protein motif:HMM:NF019016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1488 family protein"
                     /protein_id="HWX14277.1"
                     /translation="VRPFSLNPALLGWEMPLVSDGKSKPLPHSNSRSIGIWMSVEGRR
                     PIQAVRVLVSNEALSQLDPTNVRDLYAALEIFDRFRSLVETAASRKFDSHGFDAEKYE
                     GMPAVRLTTDDLT"
     gene            457..654
                     /locus_tag="VNY06_05400"
     CDS             457..654
                     /locus_tag="VNY06_05400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX14278.1"
                     /translation="MSIIGSIEATQELCADLDNLIEITKENRAPIRALSFIERARADL
                     QVYLDEIERTSSAPRAWSSSG"
     gene            675..905
                     /locus_tag="VNY06_05405"
     CDS             675..905
                     /locus_tag="VNY06_05405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX14279.1"
                     /translation="MAKNRRGSFSLKEDRQLIQMAAASATLEEAAAALRTSVKTIERT
                     AKRLGLTLKGRDGRKRLSARHADDLGLKAKGK"
     gene            939..1175
                     /locus_tag="VNY06_05410"
     CDS             939..1175
                     /locus_tag="VNY06_05410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX14280.1"
                     /translation="MTKEKKTAEELAAMILQDLSNMDGWPKRGVTVTVYGLNPWNSML
                     TFGVDAGSVSNKAELQSFCDIITERLKRLYDVWP"
     gene            complement(1798..2469)
                     /locus_tag="VNY06_05415"
     CDS             complement(1798..2469)
                     /locus_tag="VNY06_05415"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HWX14281.1"
                     /translation="MDVAGVSEASERRNLVLLSRLMSKRSPSNTERPSPGNPTLEKAL
                     LALRMQRLDEAERLASDVLKSNRGNVVAADVLGRALLMQNRPKEAVDPLQRAARRSHD
                     PAIEILLAAALDAAGRGEEAIDQLRLTAARRPPFPPAFLELGGQLGKSGRFDEGIAVL
                     ESGLAFAPDDLDLRMGLGYLHLKRNDRTKARCRAPPPNSWRAVSLASARSEVFRRFEP
                     CRFVP"
     gene            2639..>2793
                     /locus_tag="VNY06_05420"
     CDS             2639..>2793
                     /locus_tag="VNY06_05420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018647939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HWX14282.1"
                     /translation="MPMSADEGEKRQAIIDACRRMNELGINQGTSGNISLRHQDGMLI
                     TPTSVPY"
BASE COUNT          622 a          800 c          820 g          551 t
ORIGIN      
        1 gtgcgcccgt tcagcttgaa tcccgccctg ttaggatggg aaatgcccct cgtcagcgac
       61 ggaaaaagca agccgctacc gcacagcaac tcgcgatcga tcggcatttg gatgagcgta
      121 gagggccgaa ggcctattca ggcagtgcgt gtgcttgtca gcaacgaagc tctgtcacag
      181 ctcgatccaa cgaatgtccg tgatttgtat gccgctctcg aaattttcga tcggttcagg
      241 tcgctggtcg aaaccgccgc gagccgcaaa tttgatagcc atgggttcga cgctgaaaaa
      301 tacgaaggca tgccggccgt cagattgacc accgatgatt tgacataaga gcggccaaat
      361 gcgtcacgtc acagcaatcg cggtgttgct cgcaagtatc acgcgctccg aggccatccg
      421 ccgcctggtc cagctaggac ttcgaggtaa agccaaatga gtatcatcgg ttctatcgag
      481 gccacgcagg agctctgcgc cgacttggat aacttgatcg agatcaccaa agaaaatcgc
      541 gcacccattc gcgccctctc gtttattgag cgggcccgtg cagatttgca ggtctacctc
      601 gatgagatcg agcgaacatc aagcgcgccc cgcgcctggt cgagctcggg ctgaaggcga
      661 aggccgcaaa ttagatggcg aagaatcgca ggggcagttt ttccttgaaa gaagatcggc
      721 agctaattca aatggcagca gcctcagcca ccttggagga agctgccgca gcacttagga
      781 cctcggtcaa gacaattgaa cgaacggcca aaaggctggg ccttaccttg aaggggcgcg
      841 atggccgaaa acgcttgtcg gctcgccatg ccgatgatct ggggctgaag gccaagggaa
      901 aatgaccgcg aacgcgttgc cctgaaatgg aggacgcgat gaccaaagaa aagaaaacag
      961 ccgaagaatt ggcggcgatg atcctccaag acttgagcaa catggacggt tggcccaagc
     1021 gaggagtcac cgtaaccgtc tacggtctca atccatggaa ttcgatgctg acttttgggg
     1081 tcgatgccgg ttccgtgtcc aataaggccg agctgcagag cttttgtgac atcatcaccg
     1141 agcggttgaa gcgattgtac gacgtttggc cttagccctc acgggcggct ttcgcgcgct
     1201 cactgattac gctaacgtca caagtgacgc tgggcgggca tgttactcgc cgcataagaa
     1261 cctcagctag cacccggaag tttgaacgag tttcgccgcc aatagcaacc tgtggggcca
     1321 ttgtgcggct gtccaggtcg cgcttttcat aggccggaga aacaccatgg gaaaacaacg
     1381 tttggcgaaa tgcgctcccc tggcgtgcgc gggcttgtcc ccgcgtgcgc cgagttctga
     1441 ttctattagt cgttgtcgct gggaagatgc tcgcatacga ccttcattct gtcttcgggt
     1501 ttcatttcgt ggagaagcct agagactgcc ccgctaccta agtagcttcg tcaacgcagc
     1561 gtcatttgat aggcgcgtgc tcaacttgcg cgccgtcgct tcgtgccccg ctccaagcgc
     1621 ctcagtcaaa acctcggtag ctcgggaaat cacctgttgc ttcatgctag gcacagctcg
     1681 ttagcaccat gaagaaaaga tttttcggat gtaaaaaaaa atgaggatgc acggctttca
     1741 gcaagtcatc gagcgcaggt gttttggtcg agagaatcgg actttccgag ttcacggtca
     1801 cggtacaaat cgacatggct caaacctcct gaagacttcg gatcgggcgc tggcgaggct
     1861 cacggcgcgc caggaatttg gcggcggcgc tcggcagcgc gccttggtgc ggtcgtttcg
     1921 cttgagatga agataaccaa gccccatgcg cagatcgagg tcgtctggcg cgaacgccag
     1981 gccgctttcg agcaccgcga taccctcgtc aaagcgtcct gatttgccga gctggcctcc
     2041 cagttcgagg aacgccggcg gaaacggtgg ccgccgcgcg gctgtcaggc gcaattggtc
     2101 aattgcctcc tcgccccgcc cggcggcgtc caatgccgcg gcaagcaaaa tttcgatcgc
     2161 cggatcatgg ctgcgccgcg ccgccctctg caaaggatcg accgcctcct tggggcggtt
     2221 ctgcatcaag agcgcccggc cgagaacatc cgctgcgaca acattgccgc ggttcgactt
     2281 caacacatcg gaggcgaggc gctctgcctc atcaaggcgc tgcattcgca acgcaagcag
     2341 cgctttttca agcgttggat tgccggggct cggccgttcg gtgtttgagg gtgagcgctt
     2401 cgacatcagt ctcgacaaca aaacaagatt acgccgctcg gacgcttcgg agacgccggc
     2461 gacatccacg ctcccgaaag tcaggtcgag acgggcggcg ggttgtcaac tggaccaggc
     2521 tcgtagataa aattgatccg gtccaccgtt agcagctttg ataaggcagg tctatccgca
     2581 gcctctggct gcccgcaagc gcgaacgatg agggataccg catgagagtg aagtccggat
     2641 gccgatgagc gccgacgagg gcgagaaacg acaggccatc atcgacgctt gccgccgcat
     2701 gaatgagctc ggcatcaacc aggggacctc cggcaatatc agcctgcgtc atcaggacgg
     2761 catgctgatc acgccgacca gcgtgcctta cga
//
LOCUS       DATMPU010000238         4997 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_3601742,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000238 DATMPU010000000
VERSION     DATMPU010000238.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 4997)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4997)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4997
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3601742"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..119)
                     /locus_tag="VNY06_05425"
     CDS             complement(<1..119)
                     /locus_tag="VNY06_05425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011425487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HWX14283.1"
                     /translation="MFKKILIANRGEIACRIIATARRMGIAVVAVYSDADRDAL"
     gene            410..1120
                     /locus_tag="VNY06_05430"
     CDS             410..1120
                     /locus_tag="VNY06_05430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012590651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HWX14284.1"
                     /translation="MAATKTITATARAGTGKGAARHIRREGRIPGVIYGGGDAPEPVS
                     LDYREVNKLIYAGHFLTTIFELDLGGAKQRVIPRDYQLDPVKDQPLHVDFLRLKPGAS
                     LRVDVPVHFINQDVCPGIKRGGSLNIVRHAIEMRVPADAIPEAVVVDLASLQINDSVH
                     IAQISLPESCKPTQSERDFTIATIAAPLVAAEEPEAPAPAVATSAAVPAPDAKAKAAE
                     AGAAPAGAAAKVAPKGKK"
     gene            1178..1774
                     /gene="pth"
                     /locus_tag="VNY06_05435"
     CDS             1178..1774
                     /gene="pth"
                     /locus_tag="VNY06_05435"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013167272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HWX14285.1"
                     /translation="MLLFAGLGNMGKAFAGHRHNAGFMAIDAIARAYHAPAFRSRFQG
                     LASEIELTGERVILLKPQTYMNESGRSAGEAARYYKIDPSGIVVFHDELDLPPGKVRV
                     KTGGGNAGHNGLNSLTGHLGNDYRRVRIGIGHPGDKALVHNYVLSDFSNEDIPWMEAL
                     CDAIPAHAPLLAEGKDEEFQRKIHQAMEKPGFGQQAES"
     gene            complement(1824..3611)
                     /gene="aroB"
                     /locus_tag="VNY06_05440"
     CDS             complement(1824..3611)
                     /gene="aroB"
                     /locus_tag="VNY06_05440"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012591318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HWX14286.1"
                     /translation="MTIGNALETAAESDGARSARAAAVVSALGQRSIVLIGLMGSGKT
                     STGRRLAQELGLEFVDSDAEIEAAAGVSIGEIFARHGEDFFRDGERRVMARLLSRGPR
                     VLATGGGAFMSEETRSHIASSGVSIWLKADHDMLWRRVRKRSHRPLLQNDDPEKTLRT
                     LMEQRYPVYALADVTVDSKDGPHDLAIEDIITGLESLFRLPPALSPKGQQGSVSSFGA
                     RKQPLPEPARVRVALGARSYAILIGPGLIRQAGDHICRLAPRAGCAIVTDKNVARLHL
                     PALEHTLHDASIRHASVIVGAGEKSKSFEVYAEVCDALITARLERGDIVIALGGGVIG
                     DLAGFAAATVRRGMRLVQIPTTLLAEVDSSVGGKTGINSVHGKNLVGAFHQPSLVIAD
                     TATLETLPAREFRAGYAEIVKYGLLGDANFFAWLEANWRGVFAGGAPRVHAIVTSCRA
                     KAAIVGRDEYEAGERALLNLGHTFGHALERLTAYDGERLVHGEAVAIGMACAFRFSVR
                     SGLCGFTDQARVEAHLQAVGLPVRITAIPSWNAPAEAILDAMYQDKKVDHGTLTFVLA
                     RAIGDCFIAKSVDPSEIRAFLGDELRSGL"
     gene            3977..>4997
                     /locus_tag="VNY06_05445"
     CDS             3977..>4997
                     /locus_tag="VNY06_05445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012589145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HWX14287.1"
                     /translation="MFGLRYGSISILICALLGVGGCAFFPVNGPQTWDIQSGVSPTVP
                     YALVKLSPATIDVIAAHEPKGLAGAFTDRRPPQNITFGIGDVIDITIFEAAAGGLFIP
                     LEAGVRPGNFVTLPPQIVDNNGNITVPYAGLIKANGRTNVQVQNDIIEKIKNRAIEPQ
                     VVVALEQQRTSLISVIGEVNTPLRFAMPATGAQDRVLDAITRAGGIKGPGYATWVMLE
                     RGGKRATVPFENLVMDAHNNIFVLPGDRIYVYQEQQKFMAFGQFGANFNGVSQSQITF
                     DAWRLNLAEAVGKAGGMSDATADPGAVFLYRQEPREVAKLLGADMTRFDGPLVPVIFW
                     ISFRDP"
BASE COUNT         1057 a         1520 c         1403 g         1017 t
ORIGIN      
        1 agcgcatccc tatctgcatc cgaataaacg gcaacgacag caatccccat ccgccgcgct
       61 gtcgcaataa tgcggcaggc aatctcgccg cggttcgcaa taaggatttt tttaaacatc
      121 ttgaccccgt aaaggcgagc ccgggagccg cgtccataac aaagaatgag accagaggct
      181 aaataacatg ggacactatc ggctgtatgc gttccttgaa ttgatccatt ttgccgtgtc
      241 gaaaccgggc gaaccctgag ctacgttgaa tgtagccccc ataaaaagga tgcgcctcgg
      301 cattgcatcc gtgcggtcgc gccgctataa gcgcccccgg cgtcccttct ggcacccctg
      361 gaggcgccgg cgcctcgtca ttgaaattct catcttcagg atacgcataa tggccgcgac
      421 aaaaactatc acggccacgg cgcgcgcagg gacaggcaag ggggccgccc gccatattcg
      481 ccgtgaaggc cgcattccag gcgtgatcta cgggggcgga gacgctcccg agcccgtttc
      541 cctcgattat cgtgaagtca acaagctcat ctacgccggg cattttctga ccacgatctt
      601 cgagctcgac ttgggcggtg ccaagcaacg cgtcatcccg cgcgactacc agctcgatcc
      661 tgtcaaggat cagccgctcc acgtcgattt tttgcgtctg aagcctgggg ctagtcttcg
      721 cgtcgacgtg ccggtgcatt tcatcaatca ggatgtctgc cccggtatca agagaggtgg
      781 cagcctgaac atcgtgcgcc atgcgatcga aatgcgggtt ccggccgacg cgatccccga
      841 ggcggtcgtc gtcgacctcg cgtccctgca aatcaatgat tctgtgcata tcgcccaaat
      901 ctcattgccc gagagttgca agcctacgca gagcgaaagg gatttcacga tagcgacgat
      961 cgcagctccg ctcgttgccg ccgaagagcc ggaagccccg gccccggcag tggcgacatc
     1021 ggccgcggtc ccagcgccgg acgcaaaggc gaaagccgcg gaggctggcg ctgcacctgc
     1081 gggtgccgcc gccaaagtcg cgccaaaggg taagaaataa gcggccgggc gctccaaatt
     1141 cgtcgctttg cgtgcggaaa gcctttgagc aggcgcgatg cttctctttg ccggtctcgg
     1201 caatatgggt aaggcctttg cgggccatcg ccacaatgca ggcttcatgg ccattgacgc
     1261 gatcgcgcgc gcctatcatg cgcccgcgtt tcgttcgcgc tttcagggac tggccagcga
     1321 aatcgagctt accggcgaaa gggtcatttt gctgaagccg cagacctata tgaatgagtc
     1381 cggccgctcg gctggggagg cggcacgcta ttataagatc gatccgtctg gaatcgtcgt
     1441 tttccacgac gaactcgatc tgccgccggg caaggtgagg gtcaagacgg gcggcggaaa
     1501 tgccggtcac aatggtttaa attccttgac cggacatctc ggcaacgact atcggcgggt
     1561 gcggatcggc atcggccatc ccggcgacaa ggccctcgtg cataattatg tgctgagcga
     1621 cttctccaac gaagacatcc cctggatgga ggctctctgc gacgcaattc cggcccatgc
     1681 gccgcttctt gccgagggca aggacgagga atttcagcgc aagatacatc aggctatgga
     1741 aaaacccgga ttcggccagc aggctgaaag ttagacgaaa cgcctgccgc gcgcgcatct
     1801 tttcccatct acgcctcaga tactcatagg ccagaacgca gctcatctcc caaaaaggcg
     1861 cgtatttcgc ttggatcaac ggatttggca ataaaacaat cgccgatcgc ccgcgcaagg
     1921 acgaaggtga gcgtgccatg gtcgaccttt ttatcttgat acatggcgtc gaggatcgcc
     1981 tcagcggggg cattccagct aggaattgcg gtaatgcgca ccggaagtcc caccgcttgc
     2041 agatgggctt cgaccctcgc ttggtccgta aacccgcata ggccgctacg gaccgaaaag
     2101 cgaaacgcgc aagccatgcc gattgcgacc gcctcgccat gcaccagcct ttccccatcg
     2161 taagcggtca ggcgctctag cgcatgccca aaggtgtggc cgagattgag aagcgccctt
     2221 tcgcctgcct catattcgtc gcgcccgacg atagcagctt ttgccctgca actcgtcacg
     2281 atcgcatgga cccgcggcgc tcctcctgcg aagaccccgc gccaattggc ctcaagccag
     2341 gcgaagaaat ttgcatcgcc cagaaggcca tatttgacga tttccgcata gccagcgcga
     2401 aattcacgcg cgggcagtgt ttccagcgtg gcggtatccg ctatcaccag cgaaggctgg
     2461 tggaaagcgc cgaccaaatt cttgccgtgg accgaattga tgccggtctt gccaccaacc
     2521 gaggaatcga cctcggccag aagtgttgtc ggaatttgaa cgaggcgcat tccgcgccgg
     2581 accgtggcgg cggcaaatcc ggcaagatcg cctatgacgc cgccgccaag cgcgatcacg
     2641 atatcgccgc gctcgagccg cgcggtgatc aaagcatcgc aaacttcggc atagacctcg
     2701 aaggatttgg acttttcacc agcgcccaca atcacgcttg catggcgaat tgacgcgtca
     2761 tggagcgtgt gctcaagggc aggcaaatgc agccgggcga cattcttgtc ggtgacgatt
     2821 gcgcagcctg ccctcggcgc gagccggcaa atatgatctc cggcttgcct gatgagaccc
     2881 ggaccgatca atatcgcata gcttcgcgcc cctagcgcaa cacgaactcg ggctggctcc
     2941 ggcaaaggct gtttacgcgc cccgaaggag cttacgcttc cttgttgccc ttttggcgac
     3001 agggcaggag gcagccgaaa cagcgattca agacctgtaa tgatgtcctc gatcgcgaga
     3061 tcgtgtggac catccttcga atcaaccgtg acatccgcca gcgcataaac cgggtaacgc
     3121 tgctccatga gggtgcgcag ggttttctct ggatcgtcgt tttgcagcag cggacgatgg
     3181 gagcgcttgc gcacgcgccg ccaaagcata tcatggtcag ccttaagcca gatcgatact
     3241 ccagatgacg cgatgtggga cctcgtctcc tcgctcatga aagcgccgcc gccggtggca
     3301 agcacacgcg gccctctgct caagaggcgg gccatgacgc ggcgctctcc atcgcggaag
     3361 aaatcctcac catgccgcgc gaagatttcg ccaatcgaca cgcccgccgc tgcttcgatc
     3421 tcggcatccg aatcaacaaa ttcgaggccg agctcttgtg cgagtcgccg gcccgtcgat
     3481 gtcttgccgg atcccataag gccgatgagg acgatggacc gctgcccaag ggccgaaacg
     3541 acggccgcag cgcgcgcgct cctggcgcca tcactctctg ccgctgtttc caaagcgttg
     3601 ccgatcgtca tcgttcaagt attacaagcg ggcgagaacg gaataagcga tggggatgag
     3661 gaatattccg cccgcctttc ggaagcaatt cgataattac tctgtcacaa gatgaatgtc
     3721 gcgaacaacg gccgtcgctt gcgacattga ccacaattgg gtcgtatttc atgctttaga
     3781 ctgccctcat cttagctccg ttttcgcttt cgatattttg tagcctattc cgccgtctac
     3841 ctcaaaggag cgttgtgcca attttgtaac accccaagag atactgcgtc gcggcacgga
     3901 aaacggctcc gctttccctc aatgaactgc tattttccgt cagctatgga ggtaagagaa
     3961 cggcagggga attttcatgt ttggcttgcg gtatggctcc atttcgattt tgatttgtgc
     4021 tctgctgggc gttggcggtt gcgcgttttt ccctgttaat ggtccgcaaa cctgggatat
     4081 tcaatctgga gtgtccccta ccgttcccta tgcgctggta aagctttcgc ctgcgaccat
     4141 cgacgttatc gccgcccatg agccgaaagg ccttgcgggg gcattcaccg accggcgccc
     4201 gccgcaaaat atcactttcg gcatcggcga cgtcattgat atcacaatct ttgaagccgc
     4261 ggccggaggc ctctttattc cgctcgaagc tggcgtgcgg ccgggcaatt ttgtaactct
     4321 tccgccccaa atcgtcgaca ataacggaaa tatcaccgtt ccttacgccg gattaatcaa
     4381 ggccaatggc cggaccaatg ttcaggtcca gaacgatatt atcgaaaaga ttaagaaccg
     4441 ggcgatcgaa ccccaggttg tcgtggcctt ggagcagcag cgcacctcct tgattagcgt
     4501 gatcggcgag gtgaatacgc cccttcgctt cgcgatgccg gcaacaggcg cgcaagaccg
     4561 cgttcttgac gctatcaccc gcgctggagg cattaagggg cccggttacg cgacctgggt
     4621 catgctggag cggggcggaa agcgcgccac cgtacccttc gaaaatctcg tgatggatgc
     4681 ccataacaac atctttgtgt tgcccggcga ccgcatctat gtctaccagg agcagcagaa
     4741 attcatggcc tttggccaat tcggcgccaa cttcaacgga gtttcgcaat cgcaaatcac
     4801 tttcgacgca tggcggctca accttgcgga agccgtaggc aaggccggcg gcatgtccga
     4861 tgccacggcc gatcccggcg cagtgttcct ttaccgccag gagccgcggg aagtcgcaaa
     4921 gctgctcggc gccgacatga ccagattcga cggacctctc gttccggtca ttttttggat
     4981 tagcttccgc gaccctg
//
LOCUS       DATMPU010000239         3549 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_1439473,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000239 DATMPU010000000
VERSION     DATMPU010000239.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 3549)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3549)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3549
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1439473"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1673
                     /locus_tag="VNY06_05450"
     CDS             <1..1673
                     /locus_tag="VNY06_05450"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012386396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RecQ family ATP-dependent DNA helicase"
                     /protein_id="HWX14288.1"
                     /translation="QVHQLQAHGIAAAALNSSNSGEDNVAIEAGLRRGRYRLLYVAPE
                     RLLRPDMPALLRESGANVLAIDEAHCVSLWGHDFRPEYSDLAQAAQAIGNLQLIAVTA
                     TADAPTRADIVQKLFNAAPTIFVRSFDRPNIHLAIQRKLDSARQVEAMVKRHRGKSGI
                     VYCASRKGTEKLSAFLSQTGVPALPYHAGLDATLRLAHEDEFLRNDGVVIVATIAFGM
                     GIDKPNVRFVCHADLPQSVEAYYQEIGRAGRDGLPAEALTLFSDADILLRERQINESG
                     ASAERKRIEKRKLSALMGLCESARCRRQTLLAAFGEDSTPCGNCDICEGTWARFNGTI
                     AAQKIMSAIHRTKGRFFPGHLANLLVGRATAAMRRYGHDLLPTFGAGKEFNPAKWQNI
                     FYQLHAAGLIAQQPEDRDGWIITEAGRTVLSGKTPLMLRGDIELSSERTADMRRCLEE
                     IEIAETKDPALPRATFEPAKSEAKTTALTARQNSLLAVLKAKRLEVARNQKQPAFVIF
                     HDSVLIEMAIRHPATSEDLLKIPGVGPIKAARYGGIFLAAIANHNSDT"
     gene            1670..3448
                     /gene="ggt"
                     /locus_tag="VNY06_05455"
     CDS             1670..3448
                     /gene="ggt"
                     /locus_tag="VNY06_05455"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015950655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HWX14289.1"
                     /translation="MSIVSFRSRPSKVPRTAVQICVLALFCLAAPFAAWAEAPWPQPI
                     ISQGARSLPVLATHGMAVVQEGTAAKIGVDILRSGGNVVDAAVAVGFALAVTLPRAGN
                     LGGGGFMLVYLAKEKKTVAIDYRETAPADTGRDVFLDAAGELVAAKSRDSGLGVGVPG
                     TVAGLSLALRKYGSGKFSMAELVAPAVALARNGIEVEDDLADSLPLAQARLGRWPSTA
                     RIFLRPDGTSLRRGDRLVQSDLASVIEAIGRDGARAFYEGPVAEKIVASIRAAGGRMT
                     LDDLKSYRAIEREPLHGFYHGHEIIAMPPPSSGGVHIIELLNILEGFPLAAQGANSAA
                     NIHLMAEAMKLAYADRAEYLGDPDHVPVPVKGLISKTYAERLRAEISLKRARPAAEIK
                     PIDPIPYESGETTHFSIVDNDGNAVANTYTLNFSYGVGLVADGTGILLNNELDDFAAK
                     PGVPNAYGLVGGAANEPGPRKRPLSSMAPTMVFRDGELELVTGSPGGSRIITIVTDII
                     LGMLDFGMNIAEATGAVRIHHQWQPDELQVERGLSEDTIRLLEALGHKTVVRGAWGSA
                     QSIMRMNGQLMGASDTRQRGTLAIGY"
BASE COUNT          732 a         1031 c         1059 g          727 t
ORIGIN      
        1 accaagtgca ccaattgcag gcgcatggga ttgcggctgc cgcgctaaac tcatcgaaca
       61 gcggcgaaga caatgtggca atcgaggcgg gtttgcggcg cggccgctac cgcctcctct
      121 atgtcgcgcc agagcgtctc ctgcgtcccg atatgccagc actgctgcgt gaatcgggcg
      181 caaatgtgct cgccatcgac gaagcgcatt gcgtctcgct ctgggggcat gacttccgtc
      241 cggaatattc ggaccttgcg caagctgccc aagcaatcgg aaatctccag cttatcgcgg
      301 tgaccgcgac cgcagacgca ccgacgcgtg cggatatcgt ccaaaaactt tttaatgccg
      361 cgccaacaat ttttgttcgc tcgttcgaca ggccgaatat ccatcttgcg atacagcgca
      421 aacttgatag tgcgcggcag gtcgaagcaa tggtgaaacg ccacagaggc aagagcggga
      481 ttgtctactg cgcgtcacgc aaaggcacgg aaaagctcag cgcattcttg agccaaaccg
      541 gggtgcccgc gctaccttac catgctggtc tcgatgccac gctaaggttg gcgcatgagg
      601 atgaattttt gcgcaatgac ggcgtagtca ttgtcgccac catcgcgttc ggcatgggga
      661 tcgacaagcc caatgttcgc ttcgtgtgcc atgccgacct gccgcagagt gtcgaggcct
      721 attatcaaga gattggccgc gccggccggg acggattgcc ggcggaggcg ctgaccctct
      781 ttagcgacgc cgatattctt ctgcgcgagc ggcaaatcaa tgagagcggg gcttccgcgg
      841 agcgcaagcg gattgagaag cgcaagctca gtgccttgat gggcttgtgt gaatccgctc
      901 gctgccgccg ccagaccttg ctcgccgctt tcggcgaaga ctccacgcct tgcggcaatt
      961 gcgacatctg cgagggcaca tgggcgcgtt tcaatggaac aattgcagcg caaaaaatca
     1021 tgtctgccat acaccgcacc aaagggcgtt ttttccccgg ccatctcgct aatctcctcg
     1081 ttgggcgggc gaccgctgcg atgaggcggt atggtcacga tcttcttccg acatttgggg
     1141 caggcaagga gttcaatccg gccaaatggc aaaacatatt ttatcagctt catgcggcgg
     1201 gcctcattgc ccagcaaccc gaggatcgcg atggctggat cattacggag gccggacgca
     1261 ctgttctttc ggggaagacg cccctaatgc tgcgcggcga tatcgaattg tcgagtgagc
     1321 ggaccgccga tatgcggcga tgcttggaag agatagagat tgcggagacc aaggaccctg
     1381 cactccctcg tgcaacgttc gaaccggcga aatcagaagc gaagaccacg gcattgacgg
     1441 cgcggcaaaa tagcttgctc gccgtcctca aagccaaacg gctcgaagtc gcgaggaacc
     1501 aaaagcagcc agccttcgtc attttccatg acagcgtctt gatcgagatg gccatcaggc
     1561 atccggcgac tagcgaagac ttgctgaaaa ttccaggtgt tggaccaatc aaagccgcgc
     1621 gctatggcgg gattttcctt gccgctatag ccaaccataa cagcgacaca tgagcatcgt
     1681 ttcgtttcga tcaagaccct cgaaagtacc acgcaccgcg gtgcaaatct gcgtgctcgc
     1741 tctcttttgt cttgccgctc catttgcggc gtgggcggaa gcgccatggc cgcaaccgat
     1801 catttcgcag ggcgcgcggt ctttgccggt gttggccact cacggcatgg ccgtcgtgca
     1861 ggaaggcacc gcggcgaaga tcggcgtgga catactccgg agcggcggca atgtggtcga
     1921 tgctgcggtc gctgtcggct tcgcgctcgc agtgacgctg cctcgcgcgg gaaatctcgg
     1981 aggcggcggc ttcatgctcg tttatctcgc aaaggagaag aagaccgttg ccatcgacta
     2041 tcgcgaaacc gcgccggccg atactgggcg cgatgttttt ctcgatgcag cgggcgagct
     2101 ggtggcagcg aaatcgcgcg actcaggcct tggggtcggg gtacccggga ccgtcgccgg
     2161 gctttctctt gccttgcgca aatatggttc gggaaagttc tcgatggccg agcttgttgc
     2221 gccggccgtt gccctcgccc gaaatggcat tgaggtcgaa gatgatcttg ccgattccct
     2281 gcccttggcg caggcaaggt tagggagatg gccctcaacg gcgcggattt tcctgcgtcc
     2341 ggatgggacg tctctgcggc gcggcgacag gcttgttcag tccgaccttg cttccgtcat
     2401 cgaagcgatt ggccgggatg gtgcgcgcgc cttttacgag gggccggtgg ctgaaaaaat
     2461 cgttgcaagc attcgcgccg caggcggaag gatgacactt gatgacctca aatcataccg
     2521 tgcaattgag agagagcctt tgcacggttt ttatcacggc cacgaaatca tagcgatgcc
     2581 gccgccttcg tcagggggtg ttcatattat cgaactcctc aatattctcg aaggctttcc
     2641 actcgcggcg caaggcgcca actcggccgc gaatattcat ctcatggcgg aagcgatgaa
     2701 attggcttat gccgaccgcg ccgaatatct tggtgatccg gaccatgtgc cggtcccagt
     2761 gaagggtctg atctcgaaga cttacgccga acggctgcgt gcggaaattt cgctcaagcg
     2821 cgcgcggccc gcggcggaga tcaagccgat cgatcccatc ccttatgaga gcggcgagac
     2881 aacgcatttc tcgattgtcg acaacgacgg caacgcggtc gccaacacct acactttgaa
     2941 tttttcctat ggtgtcggcc tcgtcgccga cggaacagga atccttctga acaatgaact
     3001 cgatgatttc gccgcaaagc ccggcgtgcc aaacgcctat ggacttgttg gcggcgccgc
     3061 caatgagccg gggccacgca aacggcccct ttcgtccatg gcgcccacca tggtatttcg
     3121 cgatggtgaa ctggaactcg tgaccggctc gcccggcggc tcgcggatca tcacgattgt
     3181 cacagatatc attctcggca tgctcgactt cggcatgaat atcgccgagg cgaccggggc
     3241 cgtgagaatc catcaccagt ggcagcccga tgaactgcag gtcgagcgcg gcctgtcgga
     3301 ggatacaatc cggctgctcg aagcacttgg gcacaagacc gtggtccggg gtgcctgggg
     3361 ctcggcgcag agcatcatgc gcatgaatgg ccagctgatg ggcgcgtccg acacgcgcca
     3421 gcgaggcaca ttggccattg gatattgaca ctcgccaaga ggtaaaaaaa tgcccggcgt
     3481 ttgacgccgg catttaacgt tcttcttgta gttttacctt tagaatttca cgagcacgtc
     3541 gcctccaaa
//
LOCUS       DATMPU010000240         2592 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_181311,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000240 DATMPU010000000
VERSION     DATMPU010000240.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 2592)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2592)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2592
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_181311"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..970)
                     /gene="kdpA"
                     /locus_tag="VNY06_05460"
     CDS             complement(<1..970)
                     /gene="kdpA"
                     /locus_tag="VNY06_05460"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020174704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HWX14290.1"
                     /translation="MTANGFVQIALFCAVVILLVKPFGYYMTRVFSGERTFLSLPLRP
                     LERAIYALSGVKEDEEQDWKSYGLAMLIFSLAGFLALYALQRLQAVLPFNPQHLDAVS
                     PDLAFNTSVSFVTNTNWQSYTPETTMSYLTQMAGLTVHNFVSAATGIALAIALIRGFA
                     RRSARTIGNFWVDMTRTVLYVLLPIAIVVGLFFVWQGMPQNLGGYVEATTLEGAKQVI
                     AQGPVASQEVIKIMGTNGGGFFNANSAHPYENPNALTNFVQMVLIFSIGAALTNVFGR
                     MVGKERQGWAIFAVMGILFLTGVFTVYATESGGNPAFASLPIDAAPG"
     gene            complement(983..1072)
                     /gene="kdpF"
                     /locus_tag="VNY06_05465"
     CDS             complement(983..1072)
                     /gene="kdpF"
                     /locus_tag="VNY06_05465"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HWX14291.1"
                     /translation="MTFDNLLGAIVTAVVMIYLVIALIRPERF"
     gene            complement(1084..1374)
                     /locus_tag="VNY06_05470"
     CDS             complement(1084..1374)
                     /locus_tag="VNY06_05470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX14292.1"
                     /translation="MEADGILGPCRGRRPGWCAIFMEFLYLAPLSSLEFICCIRDTQS
                     FEHFQAKQAPVRLEIALEGSSMMSFALDFVFVAIGVAAFAVTALYLNACASL"
     gene            complement(1568..1810)
                     /locus_tag="VNY06_05475"
     CDS             complement(1568..1810)
                     /locus_tag="VNY06_05475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX14293.1"
                     /translation="MQSEHAREAAEYRAKGSETARTITATADRDVVVLRGNAQRQAGQ
                     SRDEGDAERNRIFAEAYGRTRISLPSIARCKPMKLD"
     gene            complement(1815..2033)
                     /locus_tag="VNY06_05480"
     CDS             complement(1815..2033)
                     /locus_tag="VNY06_05480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX14294.1"
                     /translation="MRGIAHGFEKLQTYRRVLGDANMAEIVRGNRADLMVKIRAQVNQ
                     GGGNLGVAAVDVRICRARSVTPDFGKSL"
     gene            complement(2021..2308)
                     /locus_tag="VNY06_05485"
     CDS             complement(2021..2308)
                     /locus_tag="VNY06_05485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012590590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HWX14295.1"
                     /translation="MRISSTDVIEGGRVLYAIGKIKAASTWHAANGAPLQDNWRELVL
                     SELIRRAEDIDADAIIGFDYETDGIVPVEESGVQLKRILATGIAVKLSCAA"
BASE COUNT          598 a          740 c          742 g          512 t
ORIGIN      
        1 cgcccggcgc agcgtcgatg ggcaggcttg cgaaagccgg gttgccgccg ctctcggtgg
       61 catagacggt gaacaccccc gtcaaaaaca aaatgcccat gaccgcgaag atcgcccagc
      121 cctggcgctc cttgccgacc atccggccga acacattggt gagcgccgcg ccaatcgaaa
      181 aaatcagcac catttggacg aaattggtca gggcgttggg attctcgtaa ggatgcgcgg
      241 aattggcgtt gaagaagccg ccgccattgg tgcccatgat tttaatgact tcctgcgaag
      301 ccaccggccc ctgcgcgatc acttgtttgg cgccttcgag cgtcgtcgct tcgacatagc
      361 cacccagatt ttgtggcatg ccttgccaaa cgaaaaagag tcccacgaca atcgcgatcg
      421 gcaacagcac ataaagaacg gtgcgggtca tgtcgaccca gaaattgcca atggttcgcg
      481 ccgagcggcg ggcaaagccc ctgatgaggg cgatggcgag tgcgatgccg gtcgcggcgg
      541 aaacgaaatt atgcacggtg agcccggcca tctgggtcag atagctcatc gtggtctcgg
      601 gcgtgtagga ttgccaattg gtgttggtga cgaagctcac cgacgtgttg aaggcgaggt
      661 cgggagagac cgcatcgaga tgctgcggat tgaagggaag gaccgcctgc aaacgctgca
      721 aggcgtaaag ggcgaggaac cccgccagac tgaaaatgag catggcgagg ccgtagctct
      781 tccagtcctg ctcctcatct tccttgacgc cactcagcgc atagattgcc cgctcaaggg
      841 gccgcagtgg aagcgacaaa aaagtgcgct cgccgctgaa gacgcgggtc atataatagc
      901 cgaacggctt cacgagaagg atgaccaccg cgcaaaaaag cgcgatctga acaaaaccat
      961 tggctgtcat ggcgtctctt tctcaaaagc gctcggggcg tatcaatgcg atgacgagat
     1021 aaatcatgac gaccgcggtt acgatcgcgc caagcaagtt gtcgaatgtc atggttccgc
     1081 ctctcacaag ctggcgcagg cgttaagata gagcgccgtg acggcaaaag ccgccacgcc
     1141 aatggcgacg aagacgaagt cgagagcgaa gctcatcatt gaagatcctt ccaacgcaat
     1201 ttccaggcga accggtgcct gcttcgcctg aaaatgctcg aagctttgag tatcgcgaat
     1261 gcagcatata aattcgaggc tgctcaacgg cgccagatat aaaaattcca taaaaattgc
     1321 gcaccagcct ggtctcctgc cccggcatgg ccccaggatg ccatcggcct ccatagcatc
     1381 gcgcttgctt aagattgatc ccaaagctgc cgccgcgcac cgagcatggt ccgatttatg
     1441 cggatcatgg gctagcgggg agcagacggc gcaaccgcgg cgggcgttgc cggctgacca
     1501 ttgggtgtgc caaaaaaacg gaaaaaatcc gactttgggc tcatcaccat gcgcgtgtcg
     1561 ctgcctttca atccagtttc ataggcttgc atcgagcgat agaaggcaaa gaaatccggg
     1621 tcctgccata ggcctcggca aaaatgcggt ttctttccgc atcgccttcg tcccgcgact
     1681 gaccagcctg gcgctgcgcg ttgccacgaa gtacgacgac atcgcggtcg gctgtggctg
     1741 tgatcgttcg cgcggtttca gagcctttag cgcgatattc cgcagcctcg cgcgcgtgtt
     1801 cgctctgcat ccggctaaag actttttccg aaatctggcg tgacagatcg ggcgcggcag
     1861 attctcacat cgacggcggc gacgccgagg ttgccgcctc cctggttcac ctgcgcgcgg
     1921 atcttgacca tgagatcggc gcgattgccg cgcacgattt cggccatgtt cgcgtcgccc
     1981 agcaccctcc gataagtctg caacttttcg aagccatgcg ctatgccgcg caagaaagct
     2041 tgaccgcgat gccggtggcg agaatgcgct tgagttgcac cccactctct tcgaccggta
     2101 cgataccgtc agtttcataa tcgaagccga tgatcgcgtc ggcgtcgata tcttccgccc
     2161 tgcggataag ttcgctcaaa acgagttcac gccaattgtc ttggagtggg gcgccattcg
     2221 cagcatgcca agtggaggcg gccttgatct tgccgatcgc atagagaacg cggccaccct
     2281 cgatgacgtc ggttgaactg atacgcatgt cttgccttca aattcactca atgcttaatc
     2341 gctggaggtt tcccaagact taattggccg cgagagacgt ccgtctcaaa ggtagatagc
     2401 caaaagccca ttaagaaacg agatggaaca aaggcgcgaa aaatataaat tccataagtc
     2461 gtagcattgc ctagaacgtt catgaaccgg cctatagagc ccggcaggaa ctcaggggca
     2521 ttccatcgag tcatgcgagc cgcgagagtg tgtatcgggt ccgaagattg gctccgactc
     2581 acttttggcc gc
//
LOCUS       DATMPU010000241         6289 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_1135306,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000241 DATMPU010000000
VERSION     DATMPU010000241.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 6289)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6289)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6289
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1135306"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(693..2471)
                     /locus_tag="VNY06_05490"
     CDS             complement(693..2471)
                     /locus_tag="VNY06_05490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012590458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heparinase II/III family protein"
                     /protein_id="HWX14296.1"
                     /translation="MDRASLCARGQAEWDSGALAGATLNDQLRVAGLLAARAGLALWR
                     GIGAPLRAVMGLWRRPPERLLIAPQDIRTSDPTLAADIYAGYFAFGGKIVNAHGRSPF
                     ELEAMPPAWARALAGFGWLRHLRAADTAVSRANARVLVEDFLSLAGKPSQIPAWEPEV
                     AARRMIAFMSQSPVILEGADRSFYHRFMKALGRTQVFLERKLAGGLAGEPRLLAAIAL
                     AELGLCTEGSRNLIRKGTRLLAEELERQILPDGGHISRNPEKLLDFLPDLLPLRQAYT
                     ARGLQPPPQLLNAIDRMMPMLRLFRHGDGALALFNGMGVTPPELLATILAYDDVRARP
                     LTNARHSGYQRLEGTDTIAVIDAGRPPPQIFSSRAHAGCASFEFSAGAQRLVINCGAP
                     DLNRTAAREAARMTAAHSTLIIDDTSLCRFASHKGLRKWLDDQIVAGPDKVDPERNDE
                     PLGTAVAVAHNGYEAQFGLIHERKITLHRDGRRLDGTDVLRPSGEGKKIDARPFALRF
                     HIHPNLRLKRVRDGRSALCVLPNGSRWLFETSTGQADIEESIFFAAPDGPRGCAQIAV
                     YGETGRDTVIEWSFRHIERKSPRQET"
     gene            complement(2564..3886)
                     /locus_tag="VNY06_05495"
     CDS             complement(2564..3886)
                     /locus_tag="VNY06_05495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012385048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HWX14297.1"
                     /translation="MGEECPGLAARIAAAAILSDVIARRHPLDECFAAGAALSRIGGL
                     EPCDAALTRSIATAALRRLGTIRAAFAKLLEKEPPRQASQLEWTLIAAGAQILFLDVP
                     DYAAVDLAVRATRLDTKTVPFASLVNGVLRNLIRVRETILAESDPLEGDTPEWLATRW
                     RMNYGEAGARAIAAAHREEPVLDLTVVGDPSTWAARLGAIVLPTGSLRLRSQAKISEL
                     PGFAEGRWFVQDAAAALPARLLQARPGNRVADFCAAPGGKTAQLAATGAEVTAIDRSA
                     ERLKKLAANLARLDLRADIVVADAVNLKTQSFDAILVDSPCLGTGTIRRHPDIAWIKK
                     AHDLVALTTLQARLLDKAAELVKTGGMIVYCTCSLEPEENEMQIAALLRRNPGVTRVP
                     ITPSEVGGLEELLNEQGELRTLPSHLPAPEPRLAGLDGFFAARLLRVY"
     gene            4138..4359
                     /locus_tag="VNY06_05500"
     CDS             4138..4359
                     /locus_tag="VNY06_05500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015821006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1674 domain-containing protein"
                     /protein_id="HWX14298.1"
                     /translation="MQSVEGKSRFSTNASVESKKRSEAALRALAEAEERRRRHDDDTA
                     ASGREINGRGGLDPVRYGDWEVRGIASDF"
     gene            complement(4591..5379)
                     /locus_tag="VNY06_05505"
     CDS             complement(4591..5379)
                     /locus_tag="VNY06_05505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012383835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HWX14299.1"
                     /translation="MSDYDRNQSARWGQGATRIGRAEIDQGLRSYMLGVYNNMVVGLA
                     LTGAVALGVHMLAVTTDPTQAAGQAGHLMLTSFGVALYFSPLHWLVMLAPLAFILFFS
                     FRISRMSASSARTLFFAFAAAMGVSLSTILLVYTGTSIARAFFITAAAFGGLSLYGYT
                     TQRDLSPMGAFMVMGLIGVVIAGLVNLFLHSTGLQFALSGLSVLIFAGLTAWDTQAIK
                     EMYYASDDYEVATKKSVNGALMLYLDFINIFVALLNLTGGGRND"
     gene            complement(5524..>6289)
                     /locus_tag="VNY06_05510"
     CDS             complement(5524..>6289)
                     /locus_tag="VNY06_05510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020173468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HWX14300.1"
                     /translation="IVSGAWWPQDYSGPNLVSIEAEIAEGLGLAVGDEITVNVLGRDV
                     KAKIASTRKVNWLTFGINFVLVFSPNSFVGAPVNDLATLTFASGADSSRETALVRAAS
                     GSFPSVTTIRVKDALDAVNGVVGQLAIAVRAASSVAFLASILVLGGALAAGQDARVHD
                     AVVLKTLGATRSRLLSAYIYEYGLIGVCTALFGVAAGAFGAFSIVRGLMDMEFEWLWP
                     QAFMAAAVAVVLTILLGLLGTWRILGRKPAPYLRDL"
BASE COUNT         1377 a         1860 c         1846 g         1206 t
ORIGIN      
        1 ggaacgaagg cagttccctt taggctagtc atggccgttg gcgcaggcgg gtcatcgtct
       61 ggccagaccg gacatgaaac cgaagtgacc gattcgtctg ggtaatggta aagtttgaac
      121 ttaagatcgc ttatttctaa atccgtctta ctcttgttgt ttgcgatgat gttcatggca
      181 aacgaactgc gtattttccg gcgcatttgc cattttcttt ctttgagctt tttcctggcc
      241 gcgggttcat cgcaggaaag gcggacatag attctgcgcc ggagagaaaa gagcaatcga
      301 gtgcctcccc cactcagttc tttcgaccgt tcctgaacgc tcccaagagc gcattggctg
      361 ccgctgcctg ctcacgggtt agacgaactc ccggcatgtt cgctctcccg gcatgttcgc
      421 tctcccagcc aataccttct cgccagtgtc gatgtgattg tccggctgcg cgatgaaata
      481 tccggttagc gagagcgcca tacgaatgaa agtctgggca gtttcttgcc agcgcctggg
      541 gtcttctgag acggcgctgg gcggtaaccg aacggtggct agagtgtgtt gagccaaaag
      601 tgggaactgg tattgcgtca ggacaaacaa caaaataagg attcagagca agatgacgtt
      661 tcgaacaaaa gtcatcttgt tgctctagcc ggttatgtct cttgccgtgg cgacttgcgc
      721 tctatgtggc gaaaactcca ttcgatcacg gtgtctctgc cggtttcgcc atagacggca
      781 atctgggcac aaccgcgtgg accatccggc gccgcgaaaa aaatgctctc ctcgatatcg
      841 gcttgtccgg ttgaggtttc gaacagccag cggctgccgt ttggcaagac acaaagcgcg
      901 ctgcgcccgt cgcgaacgcg cttcaggcgc aaattcggat ggatgtgaaa gcgtaacgca
      961 aatgggcggg cgtcgatttt tttgccctct ccggacgggc ggagaacatc ggtgccgtca
     1021 agcctcctgc cgtccctgtg gagtgtgatt ttgcgttcat gaatgagacc aaattgcgcc
     1081 tcgtaaccat tgtgcgcgac cgccacggct gtacccaagg gttcgtcgtt gcgctcgggg
     1141 tccaccttgt ccggccctgc aacgatctga tcgtcgagcc atttccgcaa tcccttgtga
     1201 gaagcgaagc ggcacaatga ggtatcatcg atgataagcg ttgaatgcgc tgccgtcatg
     1261 cgggcagctt cacgtgcggc cgtgcgattg agatcgggag caccgcaatt gataacaagc
     1321 cgctgtgcac ccgcagaaaa ttcgaacgac gcgcacccag cgtgcgcccg gctggaaaaa
     1381 atctgcggcg gtggccgccc cgcgtcgatg acggcgattg tgtcagtccc ctcgagccgc
     1441 tgatagcccg agtgccgcgc attggtcagc ggccgcgcgc ggacgtcgtc gtaagcaagg
     1501 attgtcgcga gtagctctgg gggcgtaaca cccatgccgt taaacaaggc aagagcgccg
     1561 tctccatgcc ggaacaggcg cagcatcggc atcatccggt caatggcgtt gagaagctgc
     1621 ggcggaggct gcaacccgcg tgcggtataa gcttgccgca ggggaagcag gtcgggaagg
     1681 aagtcaagca gcttctccgg attgcggctg atatggccgc cgtccggaag gatttgccgc
     1741 tcaagctcct cggcaagcag tcttgtaccc ttgcgaatga gattccggct gccttctgtg
     1801 caaagtccga gttcggcaag tgctattgcc gcgaggaggc ggggttcgcc cgctagtccg
     1861 ccggcgagtt tacgctccag gaagacctgg gtgcggccaa gtgctttcat gaaccggtgg
     1921 tagaagctgc gatcggcacc ttcgagaatc accggggact gagacataaa tgcaatcatg
     1981 cgccttgcgg caacttctgg ttcccatgcg ggaatttgcg aaggcttacc tgccagactc
     2041 aaaaaatctt cgacgagcac gcgtgcattg gcgcgcgaca cggcggtatc ggcggcacgc
     2101 aaatggcgca gccagccaaa tcctgcgagt gcccgcgccc aggcaggtgg cattgcctca
     2161 agctcaaaag gtgagcggcc atgtgcgttg acgatcttgc cgccgaacgc aaaatagcca
     2221 gcgtagatat ccgcagccaa ggtcggatcg ctggtcctaa tgtcctgcgg cgcaatcaaa
     2281 agccgctccg gcggccgccg ccagagcccc atgacggcgc gtaggggtgc gccgatcccg
     2341 cgccatagcg ctaatcccgc gcgagcggca aggagtcccg cgacgcgcaa ctggtcgttt
     2401 aatgtcgcgc cagccagagc tccgctatcc cattctgctt gccctctcgc acacaagctg
     2461 gcgcgatcca ttgagcaaga ctgttttcct gaaccacaat cgccttcgtt ccgcgatcca
     2521 tgcccggggc agtgagttat cattaatatg cctatcgggt ccatcaatag acgcgcaaca
     2581 atcgggcggc aaagaacccg tcgaggccgg caagccgcgg ttcgggcgcg gggagatgtg
     2641 acggcaatgt ccgcaattca ccctgctcgt tcaagagttc ctccaaaccg ccaacttccg
     2701 agggcgtaat tggtacgcgc gtcacaccag gattgcggcg cagaagagcc gcaatctgca
     2761 tttcattttc ttcgggttca agcgaacagg tacaatagac gatcattccg cccgttttca
     2821 ccagctcggc ggctttatcg agaaggcgcg cctgaagcgt tgtcagcgcc accaaatcat
     2881 gtgctttctt aatccaggcg atgtcggggt gacggcggat tgtgcccgtg ccaagacatg
     2941 gcgaatcgac caaaatcgcg tcgaatgact gtgtcttgag gtttacggcg tcggcaacga
     3001 caatgtcagc gcgcaaatcg agccgggcga gattggcggc gagctttttc aatcgctcgg
     3061 cagagcggtc gattgcggta acctccgcac ccgtcgcggc gagctgggcc gtcttgcctc
     3121 caggggctgc gcaaaaatcg gccactctgt ttccggggcg agcctggaga agtcgcgccg
     3181 gaagcgcagc cgccgcatct tggacgaacc atcggccttc tgcaaatccg ggaagttcgc
     3241 ttatctttgc ttgggatcga agccggagcg aaccggttgg caagacaatc gctccaagcc
     3301 tcgctgccca agtcgagggg tcacccacca ccgtaaggtc caggaccggc tcctcacgat
     3361 gggcagcggc aatggcgcgt gcaccagcct cgccgtagtt catgcgccac cgcgtcgcga
     3421 gccattcggg ggtatcgccc tccagcggat cgctttccgc cagtattgtt tcgcgtacgc
     3481 gtatgagatt gcgcaagacg ccattgacga gggaagcaaa cggaacggtt ttcgtatcaa
     3541 ggcgggtcgc gcggacggcg agatcgacag cggcgtaatc cggaacgtcg aggaaaagta
     3601 tctgcgcccc ggctgcgatc agcgtccatt caagctggga tgcctgacgc ggcggttcct
     3661 tttccaggag cttggcgaac gcggcccgaa tggtaccgag gcgcctcagc gcggccgtgg
     3721 caatcgagcg tgttagggcg gcatcacaag gctcgagccc cccgatccgc gagagagccg
     3781 cgccagctgc aaagcattcg tcaaggggat gtcgtctggc gatgacatcg cttagaattg
     3841 cagcggcggc gatccgcgcc gcaagaccgg ggcactcctc acccattccc ggtgaagttg
     3901 cctggttttg cgaccttgcg ttcgaagcct ttttttgatt gcgccacctg cctccaggcg
     3961 gccgcgtagt tacggtcaaa ctcaaacctc cgcaccgaca ggtccaggca agccaataga
     4021 accattttta gcatgaacag ttacactttg cccagcatgt cggcgaggat caggtaaaac
     4081 ttacgttgaa ggcgaaggca tcagcaagct gccccgccac gcagcaagga atatgagatg
     4141 cagtctgttg agggtaagtc gagattctcc actaatgcca gcgtggagag taagaaacgt
     4201 tctgaggcgg cgctacgcgc gcttgcagag gccgaggagc gccgccgccg tcatgacgac
     4261 gatacggccg cgtcgggcag ggaaataaac gggcgtggtg gactcgaccc ggtgcgctac
     4321 ggcgattggg aagtgagggg aatcgcgagc gacttctgag cgcgagacgc ttccgactca
     4381 gcgccggagc aattcggcca ttcttcaagt cggccgctcg ttctcattct ttgttttgac
     4441 atctttgttt tggcatgggt ccggccgcaa aaccggcgcc gacttttggg ctcgacgaca
     4501 ctttctcaac gcaacggcgc ggcggcttta acaaccgctc atgcaaaaaa gccccgcgaa
     4561 tgcggggctt ttccatggcc ggagccgaac tcagtcgttg cgtccgccgc ctgtcaaatt
     4621 cagcagcgcg acaaagatgt tgatgaaatc caggtagagc atcaaagccc catttacgga
     4681 tttcttcgtc gcgacctcat agtcgtcgct cgcataatac atttctttga tcgcctgcgt
     4741 atcccaggcg gtcaggcccg caaagatcag aaccgagaga cctgacagag cgaactgtag
     4801 cccggtgctg tgcaggaaga gattgaccag tcccgcaatc acgacgccaa tgaggcccat
     4861 gaccatgaac gctcccatgg gcgacaagtc acgttgcgtc gtatagccat aaaggctcaa
     4921 ccccccaaaa gcggccgccg tgatgaagaa ggcgcgcgct atggatgtcc ccgtatagac
     4981 gagcaggatc gtcgaaagcg atacacccat cgccgccgcg aaggcgaaga aaagagtgcg
     5041 cgccgacgag gccgacatcc ggcttatgcg gaacgagaaa aatagaatga aggcaagcgg
     5101 ggccagcatc accagccaat gcagcgggct aaagtagagc gccacaccaa acgaagtgag
     5161 catcagatgc cctgcctggc ctgccgcctg cgtggggtcg gtggtgacgg cgagcatatg
     5221 gaccccaagc gccaccgcac cggtgagcgc caagccaacc accatgttgt tatagacgcc
     5281 cagcatatag ctacgcaggc cctggtcgat ctcggcgcga ccgatccgtg tggcgccctg
     5341 cccccagcgg gcactttggt tacggtcgta gtcggacatg ttttcctcta aaagttgtgg
     5401 aggctgaacc gcctccggcc gctgcccttc cgggaacagc gtcgcaggta atatggccca
     5461 tgcaagcgac aggcacaagg gcagcttagc atgaacaata gttaagccat ctcaacaaca
     5521 atactataaa tcgcgcagat aaggcgccgg tttgcggcca agtattcgcc aggtaccgag
     5581 aagaccgagc aaaatggtca gcacgaccgc cactgctgcg gccatgaacg cctgcggcca
     5641 aagccactca aattccatgt ccatcaagcc gcgcacgata gaaaaggcac caaaagcccc
     5701 cgccgccacg ccaaagagtg cggtgcagac cccaatcagc ccatattcat agatgtaagc
     5761 ggataatagc cgggaccttg tggcgccgag ggttttgagc acaacggcat catggacgcg
     5821 cgcatcctga ccggcagcaa gtgcgccgcc caagacaagg atcgaagcca ggaacgcaac
     5881 gctcgacgca gccctcacag cgatcgcgag ctggccaaca acgccgttca cagcgtcaag
     5941 cgcgtccttc actcggatcg tcgtcaccga gggaaagcta ccggacgccg cacgcacaag
     6001 tgccgtttcc cgcgaggaat cggccccgct cgcaaaggtc agcgtcgcga gatcgttcac
     6061 gggcgcgcca acaaaactat ttggcgagaa caccagcacg aaattgatcc cgaaagtcag
     6121 ccaattgact ttcctagtgc tggcaatttt ggccttaacg tcgcggccca aaacattgac
     6181 ggtaatttcg tcgccgaccg caaggccaag cccctccgcg atttcggcct cgatcgaaac
     6241 caaattcggc ccgctatagt cttgcggcca ccatgcgccg ctcacaatg
//
LOCUS       DATMPU010000242         2511 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_2666208,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000242 DATMPU010000000
VERSION     DATMPU010000242.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 2511)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2511)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2511
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2666208"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..148)
                     /locus_tag="VNY06_05515"
     CDS             complement(<1..148)
                     /locus_tag="VNY06_05515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015822351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HWX14301.1"
                     /translation="MDVHEYQAKEILANFGVAVPQGSVAYSPEQAVYCATELGGWHWA
                     VKAQI"
     gene            681..1946
                     /locus_tag="VNY06_05520"
     CDS             681..1946
                     /locus_tag="VNY06_05520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018267466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HWX14302.1"
                     /translation="MERDPRQLLHAMFDAAVSAALPEKCLPPHLPPQPPGRVIVVGAG
                     KAGGSMAKAVEDNWEGSLEGLVVTRYGHGAPCQRIELVEASHPVPDAAGEEAARRILA
                     MVAGLTPEDLVLCLISGGGSALLALPAKGVTLADKQGVNKALLKSGATIAEMNCVRRH
                     LSAIKGGRLAAAAAPAHVVALMISDVPGDDPCVIASGPTVADPTSSADALAILQKYAI
                     DVPHHVRGHLASPKAETLKPGDPRLACVTNIMIATPQLSLEAAAMVARKAGVNPLILA
                     NAIEGEARDVAYVHAAIARQVHGFAQPAPPPCVLISGGETTVTVRGSGRGGRNAEFLL
                     ALGVALDGHPGIYALAADTDGIDGTEDNAGALLCPDSLARAANVGVSAKAKLANNDGY
                     SFFSALGDLVITGPTLTNVNDFRAIMVLG"
     gene            2105..>2511
                     /locus_tag="VNY06_05525"
     CDS             2105..>2511
                     /locus_tag="VNY06_05525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012590731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--glyoxylate aminotransferase"
                     /protein_id="HWX14303.1"
                     /translation="MAGRHFLFVPGPTNIPDRVTRAMTVAMEDHRSSKFPELTLPLFR
                     DLKKVFKTADGQIFIFPSSGTGGWEASLCNTLSPGDKVLASRFGQFSHLWIDLAQRLG
                     LDVQILEEEWGTGVKPDKVESVLRADKSHQIKAV"
BASE COUNT          487 a          740 c          723 g          561 t
ORIGIN      
        1 gaatttgcgc tttcaccgcc caatgccagc caccgagctc ggtcgcacaa tagaccgcct
       61 gctcgggact ataggcgacg gatccttgcg gaaccgccac accaaaattc gccaaaatct
      121 ccttggcctg atactcgtga acgtccaatc gcccctcctt gcaatccagt ttcaaccact
      181 ggccctatca attcccgcac gtctgcgcag ccgtccactc ttgctgctca tgcgtgcggc
      241 ctgcatcgct catgagccca gtcttaccct atgagcgcaa cgcttattgt caaattccag
      301 tcttagcaac cctccctgtg gcgttgcgac gcagtttagc aagcgcacca aagcgggcat
      361 aggagaaacc tccggcagaa aatcaaagct tatgacgcgc gcatatgttg gaaatcttgg
      421 tgttttcata tgtttggttg atgcctgcga tgtagagtcg gttcagaaaa tgattgaaag
      481 ctcacctatt acgtttatgt aacggcttcg ttcacgggtt catggcgttc atatgcattt
      541 attgacgagc ggctatgccc gctgctgcag tttgatcgaa acctcggacg ctttagtcac
      601 accgagtccc ggccgctcgc tacgcttccg gaacctctca aagctctccg ggtggccatg
      661 aaggaaagct gctcataatc atggaaaggg atccccgtca gttgcttcac gccatgttcg
      721 atgctgcagt cagcgccgca ttgcccgaga agtgcttgcc gccacatctc ccgccccagc
      781 cgcccggccg cgtgatcgtc gtgggcgcgg gcaaggcggg cggctcgatg gccaaagccg
      841 tcgaggacaa ttgggagggg tcgctcgaag ggttggtggt cacccgctac gggcacggcg
      901 cgccttgcca acgcatcgaa ctggtggaag cgagccatcc tgtccctgat gcagccgggg
      961 aagaggcggc aaggcgcatt cttgccatgg ttgctggcct aaccccggaa gatcttgtgc
     1021 tttgtctcat ttcaggcggc ggctcggcgc ttcttgcgtt gcccgccaaa ggggtgacac
     1081 tcgccgataa gcagggtgtc aacaaggcgc tcttgaagag cggtgcgacc attgccgaaa
     1141 tgaattgcgt gcgccggcac ttgtccgcga tcaaaggcgg gaggctggcc gccgcggcag
     1201 ccccagcgca tgtggtcgcg ctcatgatct cggatgttcc cggggatgac ccttgcgtga
     1261 tcgcctcggg gccgaccgtc gccgacccga cgagttcggc cgacgcgctg gcgatcttgc
     1321 aaaaatacgc catcgatgtt ccccatcatg tgcgcgggca tcttgcctcg ccaaaggctg
     1381 aaactttgaa gcccggcgat ccgcgccttg cttgtgttac caacataatg attgcgacgc
     1441 cgcaattgtc gctggaggcg gcggccatgg tcgcgcgcaa ggcaggcgtc aacccgctca
     1501 ttcttgccaa cgccatcgaa ggcgaggcgc gtgatgtcgc gtacgtccat gcggcaattg
     1561 caaggcaagt tcatggcttc gcgcagcctg cgccgccgcc ttgcgttctg atttcagggg
     1621 gcgaaacgac ggtgaccgtt cgggggtccg ggcgtggcgg ccgcaacgcc gaatttcttc
     1681 tcgcacttgg ggtcgccttg gatggccacc ccggtattta cgcgctcgcc gcggacaccg
     1741 acgggatcga cggcaccgag gacaatgctg gcgcgctcct ttgtcccgat agccttgccc
     1801 gcgcggcgaa tgtgggcgtg tcggccaaag ccaagcttgc caataatgac ggctacagtt
     1861 tcttctccgc tctcggagat ctcgtgatca ccggcccaac ccttaccaat gtcaatgatt
     1921 ttcgcgcaat catggtcctt ggctagctct ggtgcattgc agctaagtgt tgaaaggttc
     1981 tgatttcgtt tcggatcttg agggatgcaa ttaaggacag gaaaaccacc gcgcggaacg
     2041 cggttgcgct tgcgccggtg gttttgttag taatgtaacc cggcaaacaa atggaggatt
     2101 tcttatggcg ggccgccact ttctttttgt accagggcca accaatatcc cggaccgcgt
     2161 aacccgcgcc atgacggtgg cgatggagga ccataggtct tccaagttcc ccgagcttac
     2221 gcttccattg tttcgggatt tgaagaaagt ttttaagacc gccgatgggc agattttcat
     2281 tttcccatcc tccggcacgg gcggctggga agcttcgctc tgcaatacgc tttcccctgg
     2341 cgacaaggtc ttggcctcgc gcttcggcca attcagccat ttgtggattg atttggccca
     2401 gcggctcggc ctcgacgttc agattttgga ggaagaatgg ggtacgggtg tcaagccgga
     2461 caaagttgaa tctgttttgc gtgccgataa gagtcaccaa atcaaagccg t
//
LOCUS       DATMPU010000243         3564 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_493885,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000243 DATMPU010000000
VERSION     DATMPU010000243.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 3564)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3564)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3564
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_493885"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            220..1599
                     /locus_tag="VNY06_05530"
     CDS             220..1599
                     /locus_tag="VNY06_05530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX14304.1"
                     /translation="MYLKLTYFGLSCSDATFPFASDWLLSLANAAPLQGILQKWGFTT
                     IAGLYLEAFVIFGLVALFINPNVTSLYRLYRDRLTKAFLFDPDPDRRDKHDDLESFEP
                     KLHQICTNLCPYPIVNAALNIEGSQFANKRGRNADFFIFTPKFTGSDATRYIDTELIE
                     KDESALDLGTAMAISAAAVSSNMGSATIKLLSFTLAFLNVRLGYWLRNPRAVREDRSL
                     LSRMLDVRSFLLLKEMFGWISEKSPTVYLTDGGHIENLGVYSLMKRCCDVIIAVDAEA
                     DPGMSFDSLLKLERYARIDLGATVNLPWQKISKQALSLNNVIAEMRDSSGIPPLLGPH
                     CAACEIEYGSKQGILLYIKASLSGDEADYILDYKYRNQDFPHETTSDQFFNEEQLEAY
                     RALGFHVMQGLLTGEIPCAVEAETEEAAKKKREEILKRIHAALLGTNTKATADADGAA
                     ELERGAQIG"
     gene            complement(1587..3161)
                     /locus_tag="VNY06_05535"
     CDS             complement(1587..3161)
                     /locus_tag="VNY06_05535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020173450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucan biosynthesis protein D"
                     /protein_id="HWX14305.1"
                     /translation="MTAAIDRRALLKAVATVGLLPINAQRGFAASAMQLGPPVPFSFA
                     SLKAMARRMAHGPYIGPARPAPDIVAKINYEAWGKIKFNMERALFAEGPGKFPVSFFH
                     LGMFFPNAFEMHAVSGGSARQIIYDQSYFEMPDDSIAKKLPHGAGFAGLRVQEPRDGS
                     LDWHKNDWVAFLGADYFRAIGELRQYGLSARAVALDVAASGKAEEFPDFTQFYIEEAE
                     HGDSLTIYALLEGPSIVGAYRFVLTRTKSVFMDIDQTLFLRADVARFGLAPLTSMFWF
                     SETKKETAIDWRPEVHDSDGLALWMGTGERAWRPLNNSPRIITSSFLDENPRGFGLLQ
                     RDRSFDHYQDGVFYDRRPSLWVEPKGNWGKGAVELVEIPTDDEIHDNIVAMWAPEKAA
                     TAGSEFSLSYRLYWAADEPFPTELARCVATRLGNGGQPGQPRPKGVRKFVVEFKGPPL
                     ETLPFGVKPEPVLWTSRGTFSYIFTEAVPDDVPGHWRAQFDLTVNGPDPVEMRLYLRA
                     GDKTLSETWLYQYHPI"
     gene            complement(3266..3424)
                     /locus_tag="VNY06_05540"
     CDS             complement(3266..3424)
                     /locus_tag="VNY06_05540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX14306.1"
                     /translation="MVGASWAAGWALGGLLQLDPRISRIIEAAFALSGFVLLYFFMRT
                     AIRNEPIR"
BASE COUNT          803 a         1004 c          987 g          770 t
ORIGIN      
        1 tccttctcga tatcggcctt gaccgaagtc gtgccgctgt cgtcgccgat ggttagtgtc
       61 accggcaccg ggttcttggt acctttccgt tggtcggaaa tcgcgaagct acgccgttca
      121 gtcaaccaac gaatgagttt agcctgcccc agccctcggg gctcgcccaa cgtaattgcg
      181 gagtgccgca attcatcacc atgcgcggag ctttggctga tgtatctgaa gcttacttat
      241 tttggcctct catgctctga cgccacattc ccattcgcat cggactggct gctttcactg
      301 gcgaatgcag cgccgcttca gggcattttg caaaagtggg gcttcacaac aattgctggg
      361 ctttatcttg aggcattcgt gatctttggg ctagtagcgt tgtttatcaa ccccaatgtg
      421 acgtctctct atcgcctgta ccgcgatcgc ctcaccaaag cttttttgtt cgatcccgat
      481 cccgatcggc gcgacaagca cgatgatcta gagtcattcg agccaaagct gcatcaaatc
      541 tgcaccaacc tctgcccgta tcctatcgtg aatgcggccc tgaatattga aggatcgcaa
      601 ttcgccaata aacgaggacg caacgctgac ttttttatct ttacgccgaa atttaccggc
      661 agcgatgcaa cgcgctacat cgacaccgag ctgatcgaga aagacgagag cgctctggac
      721 ctcggaacgg cgatggcaat ttcggcggcc gccgtctcgt ccaacatggg ttcggcgacc
      781 atcaaacttc tgtctttcac gcttgccttt ctcaacgttc gtctcggcta ctggctgcgc
      841 aacccgcgcg ccgtccgcga ggaccgctcg ctcttgtcgc gcatgctgga cgtacgcagc
      901 ttccttttgc tcaaagagat gttcggctgg atttcagaaa aaagtccaac cgtttatctc
      961 accgacggcg gccacattga aaatcttggt gtctattcac tcatgaagag gtgctgtgat
     1021 gttattatcg ccgtcgacgc cgaagccgat ccgggaatga gttttgactc gctgctgaag
     1081 ctagagcgct atgccagaat tgaccttggg gcgaccgtca atttgccatg gcagaagatt
     1141 agcaagcaag cgctcagtct caataacgtc attgctgaga tgagggacag ctccggcatt
     1201 cccccattgc tcggtcccca ttgcgccgcc tgcgaaattg aatatgggtc gaagcagggg
     1261 atcctgcttt acatcaaggc gtcgctaagc ggcgatgaag cggattacat cttggactat
     1321 aagtatcgca accaagactt tccgcatgag acaaccagcg accagttttt caatgaggaa
     1381 cagttggaag cgtatcgtgc cctagggttc catgtgatgc aaggcttgtt gactggcgaa
     1441 ataccatgtg cagtggaggc tgagacggag gaagcggcaa aaaagaagcg ggaggagatt
     1501 ttgaaacgca tccatgccgc tcttcttggg actaacacga aggcgacagc agatgctgat
     1561 ggcgccgcag agctggagcg cggcgctcaa atcggatgat attggtagag ccaggtttcg
     1621 ctcaacgttt tgtcgccagc gcgcaaataa agccgcatct cgaccggatc cggtccgttc
     1681 acggtcaaat cgaactgggc gcgccaatgg cccggcacgt cgtccggcac ggcttcggta
     1741 aaaatataag aaaaggtccc gcgcgaggtc cagagcacgg gctctggctt cacgccaaag
     1801 ggcagagtct cgagcggcgg gcctttgaat tcgaccacaa atttgcgcac gccctttggc
     1861 cgaggctggc ccggttggcc gccattgccc aagcgtgttg caacgcatcg cgcgagctcg
     1921 gttggaaaag gctcatccgc ggcccaatag aggcgataag aaaggctgaa ctcggatccg
     1981 gccgtcgccg ctttttccgg agcccacatc gcgacaatat tgtcgtggat ttcatcgtcg
     2041 gttgggattt cgaccagctc gaccgcgcct ttgccccaat tccctttggg ctcgacccag
     2101 aggctcggac ggcggtcata aaagacccca tcctgataat ggtcaaagga ccggtcgcgc
     2161 tgcaacagcc cgaagccgcg cgggttttca tccaaaaagg acgatgtgat gatgcgtggc
     2221 gaattgttga gcggacgcca cgctcgctct cccgtcccca tccatagcgc gaggccgtcg
     2281 gaatcatgaa cttccgggcg ccagtcgatg gcggtctctt tcttcgtttc ggagaaccag
     2341 aacatggagg tcaatggagc aaggccgaaa cgagcgacat cggcgcgcaa aaaaagggtc
     2401 tggtcgatgt ccatgaagac ggatttggtg cgcgtcagca cgaagcggta ggctcccaca
     2461 atcgatgggc cttcgagcaa ggcatagatc gtaagcgaat cgccatgttc tgcttcctcg
     2521 atgtagaatt gcgtgaagtc aggaaattcc tctgccttgc cagaggcggc gacatcgagc
     2581 gcgacggcgc gtgccgagag cccatattgg cgcagttcgc caatcgcgcg aaaatagtcg
     2641 gcgccaagaa aggccaccca atcattttta tgccagtcaa gagatccatc gcgcggttcc
     2701 tgaacgcgca agcccgcaaa gcccgcaccg tgcggcagct tctttgcaat cgaatcatcg
     2761 ggcatttcga aatagctttg atcataaatg atttgacgcg cgctgccgcc cgagacggca
     2821 tgcatttcga acgcgtttgg aaaaaacatt ccaagatgaa aaaaactcac cggaaatttt
     2881 cctgggccct cggcaaaaag ggcccgttcc atgttgaatt tgatttttcc ccaagcctcg
     2941 tagttgatct tggcgacgat gtcaggcgca ggtcgcgccg gaccgatata gggcccgtgc
     3001 gccatgcgac gcgccatggc ctttagacta gcgaaagaga acggtaccgg cggcccaagc
     3061 tgcatcgcgc tcgcagcaaa gcccctctgc gcgttgatcg gcaaaagccc cactgtggca
     3121 acggccttca gcagggctct gcggtcaatg gctgccgtca tataattttc ctttggatgt
     3181 gcgtgccaaa tttctggcgc aacccaggct tgggtgttga caggcacaaa ctgcgccgaa
     3241 ccggcgggag gaaagcaaac agccgtcagc ggattggctc gttgcgtatc gcggtgcgca
     3301 tgaaaaaata aaggagcacg aagccgctca gagcaaatgc ggcctcgatg atgcggctta
     3361 tgcggggatc aagctgcaag aggccgccca acgcccagcc cgccgcccag gaagcgccga
     3421 ccacttcggt gccgacaagg atcgcgaccg cggtgagagt tgaaacatga atccagttaa
     3481 ttttcttttg tttagccagt gcaaggtgct cttttcacaa tcaagcttgc acgcacgatt
     3541 ctatgccagc ctgccagcct gtcg
//
LOCUS       DATMPU010000244         3981 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_183489,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000244 DATMPU010000000
VERSION     DATMPU010000244.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 3981)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3981)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3981
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_183489"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(361..1239)
                     /gene="tesB"
                     /locus_tag="VNY06_05545"
     CDS             complement(361..1239)
                     /gene="tesB"
                     /locus_tag="VNY06_05545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008196517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006637 - acyl-CoA metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase II"
                     /protein_id="HWX14307.1"
                     /translation="MSEAVAKLLQILDLERIEQNIFRGRSPQVGWQRVFGGLVISQAL
                     VASTRTVEGRAPHSLHGYFLLAGDPAVPIIYEVDRIRDGKSFTTRRCNAIQHGRAIFS
                     LSASFQIEEPGLEHAVPMPKVPSPEALPSEAEMLQRFGAVMPGAIRRWFERERPIEMR
                     PLDLSRYVTHERGALQQNIWLRASSRLPDDPGVHRAMLAYLSDMTLLDAVLAAHGHSI
                     FDTGLQVASLDHALWFHRPFRADEWLLYAQDSPSSGGARGFARGLLFSQDGRLIASVV
                     QEGLIRPRPESQSIPR"
     gene            complement(1554..1775)
                     /locus_tag="VNY06_05550"
     CDS             complement(1554..1775)
                     /locus_tag="VNY06_05550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018407812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trm112 family protein"
                     /protein_id="HWX14308.1"
                     /translation="MASKDKETGGNQAPEPSRIDPRLLEILVCPRTKTTLEYDAKNQE
                     LISRAAKLAYPIRDGIPIMLPEEARPLDG"
     gene            complement(1851..2519)
                     /locus_tag="VNY06_05555"
     CDS             complement(1851..2519)
                     /locus_tag="VNY06_05555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020173650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HWX14309.1"
                     /translation="MSINHAIGGPDELPTVFPIFPLAGVLLLPRGELPLNIFEQRYIV
                     MVDDALRGERIIGMIQPDPEGRTGTAAPALFSTGCAGRITQFAETGDGRYLITLTGIA
                     RFKLTEEITAITPYRQCRADFGPFLLDFSPRAGEDQVDRVGVLRTLRDFAETNHLQID
                     WNSINSAPNEVLVNALSMMSPCSPKEKQALLEAQDLKGRADVLVAITEMELARGRSAS
                     TPLH"
     gene            complement(2678..3292)
                     /locus_tag="VNY06_05560"
     CDS             complement(2678..3292)
                     /locus_tag="VNY06_05560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX14310.1"
                     /translation="MLVVDAGVFDMSDQRAFGHHHPGAIGKFRNHPVDGRAQGNEICR
                     DHGIVANDFAMCGEHCGNAVPRIAAAMAIFKAVPEDLRFFAGGAGGGGQAPAPEGLVS
                     PTPAGTSSRNGKVIFLKNILRPIVKIMPFEPTTMSLAPVLKAQLKSFELQRFRGECRR
                     LERRAAQLPSDQPHAGKIKPAKDCDLFSAMRSGAASFQKMEADA"
     gene            complement(3302..3517)
                     /locus_tag="VNY06_05565"
     CDS             complement(3302..3517)
                     /locus_tag="VNY06_05565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX14311.1"
                     /translation="MAIDVILGLGLFGSERGVGFSRLVDKTGQAFAVHLDVNFVPADE
                     VALETQEIGAVIPGRLKVRLDTGLMRA"
     gene            complement(3714..>3981)
                     /locus_tag="VNY06_05570"
     CDS             complement(3714..>3981)
                     /locus_tag="VNY06_05570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX14312.1"
                     /translation="EEFCLQRAANGREEIRRVTVDNLGDHVAGAKALSICGTELPDHA
                     GRRCDNARGAQELDSLLPGGNRRLRFVGLGVAIVLSRLEISIAI"
BASE COUNT          874 a         1168 c         1125 g          814 t
ORIGIN      
        1 gctcggctaa tcactagaga cccgagcccg ggaatcgtgg ccgtgctggg gtcgcccaag
       61 caagccccgg gccatcagca aaatgagcgc cgaaagagtg gggcgtcgag acgaaaacat
      121 gtgttccttg tgcttggggt cttgacgcgg ttgagaggcg tcgaagctct ctcggagaca
      181 atgctgttct tgagactcaa gcgcgggcaa gtgtttgtgg caccggcttg ccggctttcg
      241 tcgcgtgtga cggcttcgtg ctgattttat gaatgtcaaa cgctgataat tattgttgtt
      301 gtatcaagct tcctagcttg cggctcgatc ctcgaaccag tctcctcatc cgcttgcgca
      361 ctaccgcgga atactctggc tttctggtcg cgggcgaata agaccttcct gaaccaccga
      421 ggcaatgagg cggccatcct gagaaaaaag gagcccgcgc gcaaaacctc gggcgccgcc
      481 tgaactcggg ctatcttgcg cgtagagaag ccattcgtcg gcgcgaaacg gacgatgaaa
      541 ccacaaggca tgatcgaggc ttgcgacttg caggccggta tcgaatattg agtgaccatg
      601 cgctgcgaga acagcatcga gcagggtcat gtcggaaaga taagcaagca tagcgcgatg
      661 aacacctgga tcatcgggaa gccggctgct cgcgcgcagc cagatatttt gctgaagagc
      721 gcccctctca tgtgttacat agcgcgacag atcgaggggg cgcatttcga tcgggcgctc
      781 ccgctcgaac cagcgacgga tcgctcccgg cattacggcg ccaaagcgtt gcagcatttc
      841 cgcctcgctg ggaagcgctt ctggcgacgg cactttgggc attggcacag catgttcgag
      901 cccaggctcc tcaatttgga acgaggccga aagtgagaag atcgcacgtc cgtgttggat
      961 cgcattgcaa cgccgcgtcg tgaagctttt tccgtcgcgg atcctgtcga cctcataaat
     1021 aatcgggact gcggggtcgc ctgccaaaag aaaatagccg tgtaaggaat gcggcgcgcg
     1081 cccttcgaca gtgcgggtcg aagcgacaag agcctgtgaa attacgagtc cgccgaaaac
     1141 gcgctgccag ccgacctgag ggctgcgccc gcgaaatatg ttctgctcga tccgttcaag
     1201 atcaagaatt tgcaagagct tagcaacggc ttcggacatg cttttctcac ttttcgccgc
     1261 cgttcgtgag ctgcgcaagc gatacggtca agcccgaacg agatcgttct cactacatcc
     1321 gtacggccgc acaccttgct cagcagtctc ctcgtcaaaa gacagccgag tcacagcgta
     1381 gcgggtcagc cgcacacacg tccatcgccg tcgagacggc tcacggtcga tcaaaagcac
     1441 tatgagcgcg cgcgctcact ttcgccaact gcgacccaaa cccctctaaa gaaccgtgaa
     1501 aagatcagca gcgaagcgca gcgccgacgc ctttgtgccg cgcgctccgc gaactatccg
     1561 tcaagaggac gcgcctcctc gggcaacatg atcggaattc cgtcccgaat aggataggcg
     1621 agtttggccg cgcgggagat cagttcctga tttttcgcat catactccaa tgtggtcttg
     1681 gtgcgtgggc ataccaggat ttcaagcagc cgcgggtcga ttcgggaagg ctccggagct
     1741 tggttgccgc ctgtttcttt gtccttactc gccattagcc cgtcctctct tcgattggca
     1801 attcccatga aaattcgcct cggataggaa atcctaggcc gctggcttct ctagtgcaag
     1861 ggtgtcgacg cgcttctccc gcgcgccaat tccatctcgg tgatcgccac aagaacatcc
     1921 gcccggcctt ttagatcttg cgcttcgaga agcgcctgct tttctttcgg gctgcatgga
     1981 ctcatcatgg acaaagcatt gaccagcacc tcattggggg cgctgttaat actgttccag
     2041 tcgatctgta ggtggttggt ttcggcaaag tcccgcagcg tacgcaacac tccgacgcga
     2101 tcgacctgat cctcgcccgc gcgcgggctg aaatcgagga gaaaagggcc aaaatcggcg
     2161 cggcattggc gatagggcgt aattgcggtg atttcttcag ttagcttgaa cctagcaata
     2221 ccggtcaggg tgataaggta gcgcccatcg ccggtttcgg cgaattgggt aatgcgcccc
     2281 gcgcatccgg tcgaaaataa cgccggtgca gcagttcccg tgcggccctc tggatcgggc
     2341 tggatcatcc caataatccg ctcgcctctc aaagcgtcgt cgaccatgac gatatagcgt
     2401 tgctcaaaga tatttagggg caattcgccg cgcggcagga gcagaacgcc ggccagcgga
     2461 aaaatcggga acaccgtcgg cagttcgtcc gggccgccaa tggcgtggtt gatactcatt
     2521 ttgcagtcct aacctgatac gagcatttct gtccagcaag gccagggagc ggaactgtca
     2581 caggctatat cagcacgtta caatcgtgac gttttcttcg aatctagatg ggatagccga
     2641 tgtcgggttg ccagtctttt aggcaaacgc ataaacttta tgcatccgct tccatcttct
     2701 gaaatgacgc cgcgcccgat ctcatagcgg agaacaaatc gcaatctttg gcgggcttta
     2761 ttttaccggc atgaggttga tctgagggta attgggcagc ccggcgctcc agtcgacggc
     2821 attcacctct aaacctctgc aattcaaatg attttagctg tgcttttagg acgggagcaa
     2881 gagacatagt tgtaggctcg aacggcatga tcttcacaat tgggcgaagt atgtttttca
     2941 gaaagataac tttgccgttc cgggaactgg tcccggctgg agtcggagat accaaaccct
     3001 ccggcgccgg cgcttggccg cctcctcctg cccctccagc aaaaaaacgc aaatcttcgg
     3061 gcacagcctt gaagattgcc atcgccgccg cgattcttgg gactgcgttg ccgcaatgtt
     3121 ctccgcacat ggcaaagtca ttagcaacaa tgccgtgatc gcgacaaatc tcgtttcctt
     3181 gcgcacgccc atcgacggga tggtttcgga acttcccgat cgcgccgggg tgatggtggc
     3241 caaaggcgcg ctgatcgctc atatcgaaaa cccccgcgtc aacgacgagc atcttgtgga
     3301 tttacgcgcg catcaagccc gtgtcgaggc ggaccttaag gcggccaggg ataaccgcgc
     3361 cgatctcctg agtctccaga gcgacctcat ccgccgggac gaaattcaca tcaaggtgaa
     3421 cggcgaacgc ttggccagtc ttgtcaacga ggcgggaaaa acccacgccg cgctcactgc
     3481 caaagaggcc caagcccaaa atgacgtcga tcgccacctc ccactggaag cctcaggaat
     3541 cgtttccaag gcggagatgc accggttacg cagcgtactt gcgcaaggca ggacgtcgcc
     3601 gcccagaacg cccggctcgc cgcgctgcgc accgaggccg aagctgccac caaaggagtc
     3661 ctcaccggga cgacaggcgg aacggacaaa acctattccg ctcaacgcgc ggatcaaatc
     3721 gcaatggaga tttccaatct cgacaagact atcgcgacgc cgagacccac gaaacgcaat
     3781 cgcctgttgc cgccgggcag aagcgaatcg agctcttgcg ctccgcgagc attgtcgcac
     3841 cgtcggccgg catgatctgg aagctcggtg ccgcagatgg agagcgcctt ggcaccggcg
     3901 acatggtcgc cgagattgtc gactgtaacg cgccgtatct cctcgcggcc attcgccgcg
     3961 cgctgaaggc aaaactcttc a
//
LOCUS       DATMPU010000245         2191 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_4522414,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000245 DATMPU010000000
VERSION     DATMPU010000245.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 2191)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2191)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2191
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4522414"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1428)
                     /locus_tag="VNY06_05575"
     CDS             complement(<1..1428)
                     /locus_tag="VNY06_05575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012592224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HWX14313.1"
                     /translation="MNISFPAAKSFSVDRAEAVTLLLGPTNTGKTHYALERMLCYSSG
                     MIGLPLRLLAREVYNRAVEKAGAGAVALITGEEKIKPKAPHYWISTVEAMPRNLDLPF
                     VAVDEIQLAADLDRGHVFTDRLLNCRGREETLLIGAATMTGLAKNLLPVTKIRVRPRL
                     SKLSFAGDTKMTRLPRRSAIVAFSVEDVYAIAEWIRRQNGGAAVVLGALSPRTRNAQV
                     DMFQNGDVDYIVATDAIGMGLNLDVDHIAFAADRKFDGWQHRRLTPSEFGQIAGRAGR
                     HHRDGTFGTTGRCPPFDAALIEALEEHRFNDVTKLQWRNTDLDFSSLSALSASLDALP
                     KEQGLARAPVAVDQLVLEIAARDANVLRTARSGGEIARLWDCCQIPDYGKLSPSAHAE
                     LALSVFGFVARGGHIPDDWFARHVAAQDSIEGGMDILSARIAEVRTWTYISNRSRWLR
                     DPSHWQNITRRVEDRLSDALHERLTQ"
     gene            complement(1913..>2191)
                     /locus_tag="VNY06_05580"
     CDS             complement(1913..>2191)
                     /locus_tag="VNY06_05580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020174374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase C-terminal
                     domain-containing protein"
                     /protein_id="HWX14314.1"
                     /translation="KVKENLTLQGFGRHTRAEQDALAIADIDALATLLNEKAFLMGEK
                     PCGADATVFSFLACFLSPTFATKVRAAAEKHQNLVGYRDRILSRYFKI"
BASE COUNT          480 a          605 c          646 g          460 t
ORIGIN      
        1 ttgggtgagg cgttcatgca aagcgtccga cagcctgtcc tctactcgac gcgtgatatt
       61 ctgccaatgg gatggatcgc gcaaccacct ggatcggttt gaaatatatg tccatgtgcg
      121 cacctcggcg atacgtgcag agaggatgtc cattccgcct tcgatggaat cctgcgccgc
      181 aacatgccga gcaaaccaat catccgggat gtgtcccccg cgcgcgacaa agccgaagac
      241 cgaaagcgcg agttccgcat gcgccgaagg cgacagcttt ccatagtctg ggatttggca
      301 gcagtcccaa aggcgggcaa tttcgccacc tgaccttgct gtccgcaaga catttgcgtc
      361 gcgggcggca atctcgagta ccaattggtc gacggcaact ggcgcgcgcg cgaggccttg
      421 ctccttcggc aaggcgtcca gcgaggcgga gagcgcagaa agcgacgaaa agtcgaggtc
      481 cgtatttcgc cattgcagct tcgtcacgtc attgaatcgg tgctcctcca gagcttcgat
      541 aagcgcggcg tcgaagggag ggcagcgccc ggttgtgcca aaagttccgt cgcggtgatg
      601 gcggcccgcg cgccccgcga tctggccgaa ttcggaaggc gtaagacggc ggtgctgcca
      661 gccgtcgaac ttccgatcgg cggcaaaggc gatgtgatcg acgtcgagat tgaggcccat
      721 tccgatcgca tcggtggcga cgatgtaatc aacatcgcca ttttgaaaca tgtcgacttg
      781 ggcattacgg gttcgcggcg aaagcgcgcc gagtacgacg gcggcgccgc cgttctgacg
      841 ccggatccat tcggcgatcg catagacatc ctcgacggaa aaagcgacga tggcgctgcg
      901 ccggggcaag cgggtcattt tggtgtcgcc ggcaaaactc aatttggaaa gccgggggcg
      961 cacgcgaatc ttcgtaaccg gtaaaaggtt cttggccaag cctgtcatgg ttgccgcgcc
     1021 aatgaggaga gtctcctcgc ggccgcggca gttcaataac cggtcggtga aaacatggcc
     1081 ccggtcgaga tcggcggcga gctggatttc gtcaacggcc acaaatggca aatcgagatt
     1141 gcgcggcatc gcctcgacag tcgaaatcca gtaatgcggc gccttcggct tgattttctc
     1201 ttcgccagtg atgagtgcga ccgcgcccgc acccgccttc tccacggcgc ggttgtagac
     1261 ctcgcgggca agcagccgga gcggcaagcc tatcatcccg ctggaatagc aaagcatccg
     1321 ctcaagggcg taatgagtct ttcccgtatt ggttggaccg agcagcaatg ttacggcctc
     1381 tgcccggtcc acggagaatg acttggccgc cggaaaggaa atgttcattg ctcgatcggc
     1441 gcgtaagtgg attgaggtgc ggcagccttt acggcttatg gttattatcc tgccacgggc
     1501 cagaacttac gccgcgccct tctaccgacg ccttatcccc ttaagaaaca taacgcgttg
     1561 aatagaacag cctgggagtg ttctcttatg cagttttccc acagaaggct tccttgtatc
     1621 acaatggagc cgctctgtat cgcaatgaaa tcgccccaga cagcgatctt tacgatttga
     1681 acgggagacg aacgaatcgc gcccgaatcg ctgacgatag gtaaatctgt gtttgttccc
     1741 cacaatatat cggagatagg ccaatttggt ggctaggtct tgtaaagggc cggaaatctt
     1801 agcagtttgc cgagctaagt ggcggcgatg ccgctcggct gtgcgtgacg ccgctgttaa
     1861 cagccaaaac aaattttatt gggccccagt cgctacacct gaaagctaag gtctagattt
     1921 tgaaataacg gctcaggatg cggtcgcgat agccaacaag attttggtgc ttttctgccg
     1981 ccgctctgac tttggtggcg aaagttgggg agaggaagca agcgaggaaa gaaaagactg
     2041 tcgcatctgc gccgcaaggc ttttcaccca tcaaaaacgc cttttcgttc agaagggtgg
     2101 caagggcgtc gatatcggca attgcaagcg cgtcctgctc ggcgcgcgta tgtctcccaa
     2161 aaccttgcaa ggttagattt tctttaacct t
//
LOCUS       DATMPU010000246         5031 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_3296093,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000246 DATMPU010000000
VERSION     DATMPU010000246.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 5031)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5031)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5031
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3296093"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1735
                     /locus_tag="VNY06_05585"
     CDS             <1..1735
                     /locus_tag="VNY06_05585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012590430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HWX14315.1"
                     /translation="EIHLGLFPGYGGTVRLPRLIGLAESAEWIISGAQQTADKALQAG
                     AVDAVSVPEHLKLAARALLQAAIAGKEDWQARRQSMQCSLGLAKEEVASQLAGAKVQA
                     AKALPHLPAADIAVALLEKASGLDRDAALALEAETFGTVAKTQTASSLVAIFVNEQAV
                     KKAIRRYAKDAPKVAKAAVVGAGIMGGGIAYQSALSGVPIVMKDISQAALDLGMSEAR
                     KLLAKSVETGRLTEQKADEIISTISPVLTYEGFEDADVVVEAVVENLGVKQKVLREIE
                     ALTSANTVLASNTSSLRIATLAEGLNCPENFLGMHFFNPVPRMPLVEVVRGPKTSAKV
                     VAAVTGFAAAMGKTPIVVEDCPGFVVNRVLTPYFIAFLHLIHEGVDFRDIDRAMEAFG
                     WPMGPAYLMDVIGMDISGHVVDIVSAGYGQRIEVPFETAIQTLLRAGRFGQKNGRGFY
                     KYEPDAKGRPRKDFDEDTARLLAPVQPRGKRNIDAGEITARMMLPLILEAARCAEEGI
                     AASPGEVDMCLILGLGLPRYLGGALKYADYLGLTNVISRADNWADLSPIYRPSERLRA
                     MAGTKDAFYSN"
     gene            1732..2589
                     /locus_tag="VNY06_05590"
     CDS             1732..2589
                     /locus_tag="VNY06_05590"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024967.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HWX14316.1"
                     /translation="MSDIKGSSILAYGKIVKRNAAHRENEHMSLAVKENVGRSGVERV
                     ISNLTAGVYSFGSLSGAVLHELDKLARSLGDNCRSLETGCGRSTIMFSNLARRHLCFC
                     FDDRNAQLPAGRDDNFGTVSFVQANPHFNKDCVTFVFGPTQKTVPNYDFRDVSLDFVF
                     LDGPHAFPFAELEYYFVYPYIRKGGILGIDDINIPTLANFFRFLRDDAMWRLISVVEN
                     TAFFERTEAPTFDPIAGIGGHNWQRYNTLHFPAHPILSRNIYLKYIQPVGRYLPASAK
                     NRLKNILSR"
     gene            2703..3686
                     /locus_tag="VNY06_05595"
     CDS             2703..3686
                     /locus_tag="VNY06_05595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012589359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HWX14317.1"
                     /translation="MLSFHTPSKTQIVTAANAKTIPADVNWIDALRPTEEEIAFLERS
                     LGIKVPNLQSLSEIQSSSRLRIEKDWLRISIPMIYRPEGFMPSLTPLGFLLSKDLLLT
                     VRFKQMKAFDDCEDDLARYPLQAGGPGALASLLEVIIDHAADTLERLASDLDLLSEQI
                     FQTHKNDAKLHGPREDSERLRVIMRKLGVSGDLSSKVEDVLLGTARMVPYVTTNAAPY
                     LDADMRKKLKSLQGDIDSLKDYEMVLADKIHFLLDATLGLTNVEQNNIFRILTVVSVV
                     GIPPTFFASMWGMNFKNIPEYDWSHGYQFGLCVIALSAIIPFVWFKRRGWW"
     gene            3716..4465
                     /locus_tag="VNY06_05600"
     CDS             3716..4465
                     /locus_tag="VNY06_05600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014892558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pseudouridine synthase"
                     /protein_id="HWX14318.1"
                     /translation="MSEEELAARLLYRDGMMLVIDKPPGLEVHRGPKGGACLEDYFDA
                     LRFGLPRKPALVHRLDRDTSGCLVLGRHHKALEKLSLLFKQGKVEKSYWAVVEGGPAQ
                     DEGVIDLPLGRLDAGRGWWMKADPFGLPSQTSWRVLSRTKNFAVLELKPLTGRTHQLR
                     VHCVSEGWPILGDPIYGHGGAMGLQLHARAIALPLYKNKEPVIAAAPLPAHMRETLAQ
                     FHSSGYFWNGDRPSQKFFFASVIVGRPWPRL"
     gene            4538..5031
                     /locus_tag="VNY06_05605"
                     /pseudo
     CDS             4538..5031
                     /locus_tag="VNY06_05605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012455634.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         1109 a         1406 c         1379 g         1137 t
ORIGIN      
        1 tgaaatacat ctcgggcttt ttcccggtta tggcggcacg gtccgcctgc cgcgcctgat
       61 cggcctcgcc gaaagcgcag agtggatcat ttcgggcgcg cagcaaaccg ccgacaaagc
      121 tctgcaggcg ggcgccgttg atgccgtttc cgtgccagaa catttaaaac tagccgcgcg
      181 tgcgcttttg caggccgcca ttgcgggcaa ggaggattgg caagcacggc gccaaagcat
      241 gcagtgcagt ctcggtctcg ccaaggagga ggtcgcgtcc cagctcgccg gagccaaggt
      301 acaagctgct aaggcgctgc cgcatctgcc cgcggcagat atcgcggtgg cgttgctcga
      361 aaaggcctca ggtcttgatc gcgacgcggc cttggcgctg gaagccgaga cttttggcac
      421 agtcgcaaag actcaaaccg ccagctcgct ggttgcaatt ttcgtcaatg agcaagccgt
      481 gaaaaaagcc atccgccgct atgccaagga tgctccgaag gtcgccaaag cggcggtcgt
      541 gggggcagga atcatgggcg gcggcatcgc ctatcaaagt gcgctcagcg gcgtgcccat
      601 cgttatgaaa gatatttctc aagcggcgtt agaccttggc atgtccgaag cgcgcaagct
      661 gctcgccaaa tccgttgaga ctgggagact aaccgagcaa aaagccgacg agataattag
      721 cacaatctcc cccgtgctca cctatgaagg ttttgaagac gcggatgtgg ttgtcgaggc
      781 cgtcgtggaa aaccttggcg tcaagcaaaa agttctgcgg gaaatcgagg cgcttaccag
      841 cgctaacact gtccttgcct cgaatacctc ctccttgcgg atcgccactt tggcagaagg
      901 ccttaactgt ccggaaaatt ttctcggcat gcattttttt aatcccgtgc caagaatgcc
      961 gctcgttgaa gtcgttcgcg gccccaaaac ctcggccaag gtggtcgcgg ccgtcaccgg
     1021 ctttgctgcc gccatgggca agacgccaat cgtggtggag gattgcccgg gcttcgtcgt
     1081 caatcgggtg ctgacgccct atttcatcgc ctttctgcac ctcattcatg agggcgtcga
     1141 ttttcgtgac atcgacagag cgatggaagc ttttggctgg cccatgggtc cggcttacct
     1201 catggatgtc atcggcatgg atatttcggg ccacgtcgtt gacatcgtat cggcgggata
     1261 tgggcaacgc atagaggtgc ccttcgagac ggcgatccaa accttgttgc gcgccgggcg
     1321 ttttggacag aaaaacggcc gcggctttta caaatatgag cctgatgcga aaggccgccc
     1381 gcgcaaggac ttcgatgagg acaccgcgcg acttctcgcg ccggtgcagc cccgtggcaa
     1441 aaggaacatt gacgcgggag agatcacggc gcgcatgatg ctgcccttga tccttgaggc
     1501 ggcgcgctgc gccgaggaag gcatcgccgc ttcgccgggg gaggtggaca tgtgcctcat
     1561 ccttggcctc ggcctgccgc gctatcttgg cggcgcgctc aaatatgccg attaccttgg
     1621 tctcaccaat gtcatctctc gtgccgacaa ttgggcggat ctcagcccca tttaccgacc
     1681 gagcgaacgc ctgcgcgcca tggcggggac aaaagatgca ttctattcca attgagtgat
     1741 attaaaggca gtagtattct ggcttatggc aagattgtta aacgaaatgc agcacatagg
     1801 gagaacgagc acatgtctct tgcagtgaaa gaaaatgttg ggcgcagcgg agtagaacgc
     1861 gtaatttcca atttgacggc aggtgtttac tcctttggat cgctcagcgg cgctgttctc
     1921 catgaactgg acaaacttgc tcgtagtctt ggcgataact gccgatcact tgagactgga
     1981 tgtgggcggt caacaattat gttctccaac cttgcgcgcc gccacctttg cttctgcttt
     2041 gatgacagaa acgctcagct ccccgccggt agagatgaca atttcgggac cgtgagtttt
     2101 gtgcaagcaa atccgcattt caacaaagat tgcgtaacat tcgtttttgg tccaacccaa
     2161 aaaacagttc caaactatga tttcagagat gtctcgttag attttgtatt tcttgacgga
     2221 ccacatgcct ttccatttgc agagttggag tattacttcg tgtatcccta tattcgaaaa
     2281 ggaggaattc ttggaatcga tgatattaat atccctaccc tcgcaaattt cttcagattt
     2341 ttgcgtgatg atgcaatgtg gagactgatt tccgtagtcg aaaatacggc cttctttgaa
     2401 agaaccgagg ctccgacgtt cgatcccatc gccggcattg gtggtcacaa ttggcagcgg
     2461 tacaatacat tgcactttcc cgcgcaccct atcttgagta ggaatatata tttgaagtat
     2521 atccagcctg tgggtcgata cctacctgcg agcgcgaaga atcgtttgaa gaatattctt
     2581 tcgagatgat aactaggtat cccaaacctc ccgaatccat attcggctgt actttgctcc
     2641 cgccccgcca gagtatccga taggctcgtt accgatacgc ttgctgttct ggacgaatcc
     2701 gcatgctcag cttccacacc cccagcaaga cacagatcgt aaccgcagcc aacgcaaaga
     2761 ctatcccggc cgatgtgaat tggatcgacg ctctgcggcc aaccgaggaa gagattgctt
     2821 tcttggaacg ctcgctagga atcaaagtgc ctaacctcca atctctctct gaaatccaat
     2881 cgtcgagcag actgcgcatc gagaaggatt ggctccgtat cagcattccg atgatctatc
     2941 ggccggaggg tttcatgccg tcgctgacgc cgcttggttt cctcttgtcg aaggatttgc
     3001 ttctgacagt ccgtttcaag caaatgaaag ctttcgacga ttgtgaggac gatctcgccc
     3061 gctatcctct gcaagccgga gggccagggg ccttggccag ccttttagaa gttattatcg
     3121 atcatgccgc agacactctg gaaaggcttg ctagtgatct cgacctcttg tctgagcaaa
     3181 tttttcaaac ccacaaaaac gacgcgaagc tccatggacc acgcgaggat agcgaacggc
     3241 ttcgtgtgat catgcgaaag ctcggagtca gtggcgatct ctcctcgaaa gtcgaagatg
     3301 ttctgctcgg cacggcgcgc atggtgccct atgtcacgac caatgcggct ccctatctcg
     3361 acgccgacat gcgcaaaaaa ctcaaaagcc tgcaaggtga catagactcc ctcaaggatt
     3421 acgagatggt tctggcagac aagatccact tcttacttga tgccacattg ggtctgacca
     3481 acgtcgagca gaacaacata tttagaattc tgactgtcgt ctcggtcgtt ggcattcccc
     3541 caaccttttt cgccagcatg tggggcatga actttaaaaa tattcccgaa tatgattggt
     3601 ctcatggtta tcaatttgga ctttgcgtca tagcactgag cgcgatcatt ccttttgttt
     3661 ggttcaaacg gcgcggctgg tggtaaaggc gcaggggccg tcacaggccg ctaccatgag
     3721 tgaggaagag ctggccgcac ggcttcttta tcgcgacggg atgatgctcg tcattgacaa
     3781 gccgcccgga ctcgaggtcc atcggggtcc gaagggcggc gcttgcctcg aagactattt
     3841 tgatgcgctg cgctttggcc tgccccgcaa acccgccctc gtccataggc tcgatcgcga
     3901 cacatcgggc tgccttgtgc tcggacgcca ccacaaggcg ctcgaaaagc ttagcctcct
     3961 gttcaagcaa ggcaaggtcg agaagtccta ctgggccgtc gtcgaaggag gtcccgctca
     4021 agacgaaggt gtgattgatc tgccgctcgg gcggctggat gccggccgcg gctggtggat
     4081 gaaggccgac cctttcggtt tgccctcaca aacgtcttgg cgcgtgttga gccgcacgaa
     4141 gaattttgcg gtgctagaac tcaagccgct gaccgggcgc acgcatcagt tgcgagtcca
     4201 ttgcgtgtca gaaggctggc caattctcgg cgatcccatc tacggacatg gcggggcaat
     4261 gggactgcag ctccatgccc gcgcaatcgc ccttcctcta tacaaaaaca aggagccggt
     4321 gatcgccgcg gcgcctctac ccgcgcatat gcgcgaaaca ctcgcgcagt ttcattcttc
     4381 aggatatttt tggaacgggg acaggccaag ccaaaagttc ttcttcgcga gtgttatcgt
     4441 cggacggcct tggccacgac tttaggttaa aaacctattt aaagggattc ccgaatcggc
     4501 gtaaggatga ttcaaccttc agaccttgga ggttgagatg gctcgttttg atctgacgga
     4561 tttcgagtgg tcggtgattt cgccgctctt gccgaactgg gccgatactc ccgagcgcta
     4621 cggccgcacg cgacctgcgt gaaccgcttc aaccgctggc gcaaggatgg cgactgggat
     4681 cgcataccga aagctgtttc aaaggcttgc gacggcgatg tccagatgac cgactcgtcc
     4741 tttatccgcg tgcatcagca cgcctccaac acctaaaaaa aaccgccgat ccggttgcat
     4801 gggtcactcg cgcggcggac tgccatcaag atccatgcgc tcgtcgacgc cgagggcctg
     4861 ccgatcgctt taaagctgac cgaaggccag gcccatactg gacgcagcgc ctgcgatatg
     4921 ctcgatgcgc ttgtcgaggg tcagaacctt ctggcatacc gacgcgctgc gccagagcct
     4981 cgccacgcgc ggcgcctttg ccaacatcaa gcccatgccg aaccgcaaga a
//
LOCUS       DATMPU010000247         4080 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_3614775,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000247 DATMPU010000000
VERSION     DATMPU010000247.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 4080)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4080)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4080
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3614775"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            128..679
                     /locus_tag="VNY06_05610"
     CDS             128..679
                     /locus_tag="VNY06_05610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX14319.1"
                     /translation="MMHARFDSIDGLYRQEEVDEAIARGTAVCLVETAKEADKLWSRE
                     VPATCCPGGSKKWRPEYSRMLSDADVVLLQYNGSSGRQRIDQISETLHGIARGIWILD
                     SKLIQADSTADEVWTTIQAAPSWAPAATTKEPYLAAARRLVYYADFGKVVKLDWIIKG
                     ILAKAHTSYLFGPPGRRKVRALG"
     gene            679..1569
                     /locus_tag="VNY06_05615"
     CDS             679..1569
                     /locus_tag="VNY06_05615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX14320.1"
                     /translation="MPRLISAFGQEWHGFKIKKPYATVYFALERADLVQKRIWAECQR
                     DGFNDVPVAVCPGLINIMDPKCVNAMVGTILAAEDKFGIEVAPAIIDTFGKAIACGGG
                     DENLASDQNIAWGHLRRVHEAMARWHAIHIAAIGHTGKDESRGARGSNAADGDNDVSL
                     QIKNEDAIKSVSIYKANDLPEGPLLRFKMAPYDTGLKDDNGDAVAVWIADRETIQAVP
                     TVIGPNLTKNQHTLFSILYRAGIVGLSTEECNNKAREIGIGVKRNADLYDVRDQLRSK
                     KLVRESAGRWFVDHKSEDPK"
     gene            complement(2216..2389)
                     /locus_tag="VNY06_05620"
     CDS             complement(2216..2389)
                     /locus_tag="VNY06_05620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX14321.1"
                     /translation="MSDNMKALQFALADLEQAQKAGERLSDKPAHRQIRERLERLIVD
                     VRSEIDQLRKETG"
     gene            complement(2685..2885)
                     /locus_tag="VNY06_05625"
     CDS             complement(2685..2885)
                     /locus_tag="VNY06_05625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX14322.1"
                     /translation="MRHFYDANSFGDENFGTHYVVLAHELKLADASALKSDAQHSEMR
                     WWDERDLLASDQVHENTKAYFR"
     gene            complement(2869..3138)
                     /locus_tag="VNY06_05630"
     CDS             complement(2869..3138)
                     /locus_tag="VNY06_05630"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HWX14323.1"
                     /translation="MLLNPDDFHSVVRLAPLVSIDLIIRNARGEVLLGLRNNEPAKGL
                     FFVPGGIIRKQERLHEAFARILKRETNYDAPLEDARLLGVYEAFL"
     gene            3833..>4080
                     /locus_tag="VNY06_05635"
     CDS             3833..>4080
                     /locus_tag="VNY06_05635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX14324.1"
                     /translation="MKAGGVGNQTEGTFRESGQLKHRVTRTIGIRPISDQLDILTKGS
                     CNTHKCWKKRFRGIFRTTDGQQLESFDAHTTVSFARRTG"
BASE COUNT          902 a         1152 c         1151 g          875 t
ORIGIN      
        1 aactaatttt ggttgaaaat ggcatgctga cgccggaaga ggcgttcgac gatttgctcc
       61 tgtggctgtc atgcacttgc acgcgcgaaa tggtcgagcg ttgggagctt caggatagga
      121 ggcgtcgatg atgcacgctc gctttgacag catcgacgga ctctatcgtc aggaggaggt
      181 ggacgaagcg atagccagag gaactgccgt ctgcctcgtc gagaccgcga aggaggctga
      241 taaattgtgg tcgcgcgaag tccccgccac ttgctgcccg ggcggctcca aaaaatggcg
      301 gcctgaatat tcaagaatgc tgagcgacgc cgatgtcgtc ctacttcaat acaatggcag
      361 ttccgggcgg cagcggatcg atcagatcag tgagacgctc catggcattg caaggggcat
      421 ttggattctc gactcgaagt tgattcaagc cgacagcacg gctgatgaag tatggacaac
      481 tatccaagcc gcgccgagct gggcaccagc ggcaacaacg aaagagcctt acttagccgc
      541 agcacggcgg ctcgtttatt acgccgattt tggcaaggtc gttaagctcg attggatcat
      601 caagggcatt ctggccaaag cgcacacttc atacctattt ggtccccccg ggcggcggaa
      661 agtccgcgct ttggggtagt gccgcgactt atctcggcgt ttggacaaga atggcacggc
      721 ttcaagatca agaagccata cgcgacggtc tatttcgctt tggagcgtgc agacctcgtc
      781 caaaagcgta tttgggccga gtgccaacgc gacggtttta atgatgtccc ggtcgcggta
      841 tgccccggcc tcatcaatat catggaccct aaatgcgtga acgcgatggt aggtaccatc
      901 ctggccgccg aagacaaatt cggcatcgag gtagcacccg caatcatcga tactttcggc
      961 aaagccatcg cctgtggtgg aggtgacgag aatctggcga gcgatcagaa catagcgtgg
     1021 ggacacttac gccgcgtcca tgaagccatg gcgcgctggc acgcgataca tatcgccgcc
     1081 ataggtcaca ccggcaaaga cgaaagtcgc ggcgcgcgtg gctccaacgc cgccgacggc
     1141 gacaatgacg tgtcgctcca aatcaaaaac gaggacgcga tcaaaagcgt gtcgatctat
     1201 aaggccaatg acttgcccga aggcccgctt ttgcgtttca aaatggcgcc gtacgatacc
     1261 ggcctcaaag acgataacgg cgatgccgtt gcagtgtgga tcgccgatcg cgagacaata
     1321 caggccgtac caaccgtaat cggccccaac ctgacgaaaa accagcacac tctattctcg
     1381 atattgtacc gtgctgggat agtcggcctt tcgaccgaag aatgtaacaa taaagcgcgc
     1441 gagatcggca tcggcgttaa gcgcaatgcc gacctctacg acgttcgtga ccagctcagg
     1501 agcaaaaagc tcgtccgtga atctgccggc cgctggtttg tggatcacaa aagcgaagac
     1561 ccaaaatgag atacggcttg cctatacgac ccgtatacga cccgtatcgg agccgtatac
     1621 ggatatacga tacgggtcgc ccccctatag gggcgaaccc gtacgtatct acgggccgaa
     1681 cggtcatgac catcggcagc ggaagtcttt ccactgttgc ggcgctgcaa taggcaaacg
     1741 gacttggaat cgctagttct ctcatcgcgt tcggcgggaa caggggcatt cacatcctgg
     1801 gccgctacgg tgagggcaaa acacaatggc tgcaaagcta agggcggtgc tgcgaggcac
     1861 ctggccgccg gctagcgttg gcttggcggt attcgtgaac tgcatctgga ttattgcgat
     1921 tggatacgga ataatctcga tgctattctg agcgaactcc caagtaattg aaactgagtc
     1981 acttttaaat tcgcaccaca gcgccttttg acaaaagccg cgaatgatca gatgattcag
     2041 tgcgccgccg agactgggaa cgccggcggt ctcacaggga caacgtgggc ggcgtgctca
     2101 acttcaaacg cacctatggc gtggccttgg attggctctt tgccggtgcc ggcgagccgc
     2161 cgccgtttcg attgcgggtt gtttcgttcg tcagggcggg ccggcgctcg cctcgctatc
     2221 cagtctcttt tcgcaactga tcgatttcac ttcttacatc gacaatgagc ctttccaggc
     2281 gctcccggat ctgcctatgc gccggcttat ccgaaagcct ctccccggcc ttttgagcct
     2341 gctcgagatc ggccagcgca aactgcaacg ccttcatatt gtcggacatt gtcctctccc
     2401 taccggcgcc gtcatgaatt cgctccatgg cggggcatat tccatatcgc tccggcgctc
     2461 ccacagtctt gccgctgatg aagatgctcc gccccggtca gttccagtgc tggccggggt
     2521 tttcgtttga ctcccgctga gcattcggac tgaacagcga ctcgagaggg ctcattttgc
     2581 cctgtcatct gcacaacgca actgacttag ccccggcccc gtctgtgctg gggcttttca
     2641 ttgcggtggc cgttgctgca aaaagcgcgt ccgttgactt gcgatcagcg aaaatacgcc
     2701 tttgtgtttt catgcacctg atcagaggcc aacagatcgc gctcgtccca ccagcgcatt
     2761 tcgctgtgct gggcgtcaga ttttagcgca gacgcatcgg caagcttgag ttcatgcgcc
     2821 agcacgacat agtgcgtgcc aaaattctca tcgccaaagc tgttggcatc atagaaatgc
     2881 ctcataaacg ccgaggagcc gcgcgtcctc aagcggcgcg tcgtaattgg tctctctctt
     2941 gagtatccgc gcaaaggcct catggaggcg ctcttgcttc cgtatgatgc cgccgggcac
     3001 gaaaaacagt cccttcgccg gctcgttgtt gcgcaacccg agcagaacct cgccgcgcgc
     3061 attgcggatg attaaatcga tggagaccag cggcgccagc cgcaccacag agtgaaaatc
     3121 gtcaggattt aaaagcatcg ctgagaccgt tcatccgatt actgttgcaa cgcggcagga
     3181 ggcgggccgg cgctcgcctt tgccgttgac gcccgctgaa tgttcggccc gaataggcgg
     3241 gccctgtttg ccaaacttag ccccggtcaa cgctcccacg ccagctgggt tttttatgtt
     3301 gagcgtggcc atcagaaaga accccaagcg tgacggtttg gggaccatta gcacgcttgg
     3361 ggccaagaca gctttcgtcg gggtgggacg ggggagctgt ctcgcagcca gcaaataagg
     3421 cgacaagcag caaatctagc gacacgtcgg attaaggttg acgggggcag tgcgctcccc
     3481 tcccgcccgt tgactccccc tgagcgttcg gcctgaatag gcgcgcatca gtctgccaac
     3541 ttagccccgg tcgtgttcca gttcggccgg ggttttcgtt tgactcgcac tgagcattcg
     3601 gactgaatgg ggcgtctgaa aatggattta acttagcccc ggtccacgct cccacgttgg
     3661 ctggggcttt tcattgcggc ccttagatgc gcatcctttt caattatgtg caggcggccg
     3721 cgtgtagggc gctggcttaa attcttccct ttcttcccgc tgatgggaag aaatctgccg
     3781 cacagagcaa aaagcctgca gttcacgttt tgcgaactcg cccgaacgtt tcgtgaaagc
     3841 tggtggcgtc ggaaatcaga ctgaaggaac gtttcgtgaa agcgggcaac tcaagcatcg
     3901 tgtgacacga acaattggta tcagaccaat atcagaccaa ttggatatac ttacaaaagg
     3961 ctcttgtaac acacataagt gctggaagaa acgtttccga ggcatttttc ggacaacaga
     4021 tggacaacaa ttggaaagtt ttgacgccca cacgacggtc tcatttgccc gccgcaccgg
//
LOCUS       DATMPU010000248         8751 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_1451557,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000248 DATMPU010000000
VERSION     DATMPU010000248.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 8751)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8751)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8751
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1451557"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..218)
                     /locus_tag="VNY06_05640"
     CDS             complement(<1..218)
                     /locus_tag="VNY06_05640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX14325.1"
                     /translation="MLNAMHIPELRARHRLAMANPEVRAKMSAVRRLAMAPGSPIRDK
                     IGSKLGWLSLKTSNDIVERLRLGDNYGK"
     gene            complement(212..427)
                     /locus_tag="VNY06_05645"
     CDS             complement(212..427)
                     /locus_tag="VNY06_05645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX14326.1"
                     /translation="MESFIKGKPVVYSGLDGAIYVAIFADGERNQLGIPTLTIENAAG
                     EIVRDIIMGEDRARSFFWNLDAVAAPC"
     gene            complement(465..656)
                     /locus_tag="VNY06_05650"
     CDS             complement(465..656)
                     /locus_tag="VNY06_05650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX14327.1"
                     /translation="MGKRNREINKGRKEAGKFQCDDPELEPDWEGQCEVCGQSPVVPL
                     TGMCGPCTFGAADTIGGNW"
     gene            complement(820..1041)
                     /locus_tag="VNY06_05655"
     CDS             complement(820..1041)
                     /locus_tag="VNY06_05655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008598879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HWX14328.1"
                     /translation="MAVTGKVKFFNEQKGYGFFTRDDGGGDVFVHRTDLPATPGAILE
                     GDPVSFEVERTHRGLRATNWSCQEPRKYS"
     gene            complement(1567..2052)
                     /locus_tag="VNY06_05660"
     CDS             complement(1567..2052)
                     /locus_tag="VNY06_05660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX14329.1"
                     /translation="MQTATIAVTYVGPPKQGKQYGFIKGADNTTFPVKQDRIREFEPG
                     NTYELAYTEGTNGFKNIIGVKRIVQQAEPRGEYTEITEPTQRRNGATLTPAVTQAKPQ
                     APQNGYYRPTAPKDSERMWTCAILGHIIDRGGVELNEASLIDAVNTLRGVYQKTYGRD
                     D"
     gene            complement(2057..2794)
                     /locus_tag="VNY06_05665"
     CDS             complement(2057..2794)
                     /locus_tag="VNY06_05665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX14330.1"
                     /translation="MHAPIGHNKPPSPIESAKEAMAELSVFTENNPVIENFDAAKQYA
                     AYIERTRVALQAMEDERKPQADPLNAKLEAINKPYRLVRQPLEKLYELAKARLSKFNN
                     AVEAARLREAQRLREEAERQEREAREAEAREQEAIHNAEQGECTDVGGAIADADQAFK
                     GFQKADRAAATAERNVSVRIASVMGNKSLSMRTTEVFTVEDACAAITIMGCSDDLKKQ
                     IIKDAKRFREATGELPEGVKSTYERSL"
     gene            complement(2804..2989)
                     /locus_tag="VNY06_05670"
     CDS             complement(2804..2989)
                     /locus_tag="VNY06_05670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX14331.1"
                     /translation="MDEQKKVEAARGVRRRHKADPGECAYCDRERKAINEFHPPHDAS
                     DRCRSGKRPHCSCDTCF"
     gene            complement(3057..3515)
                     /locus_tag="VNY06_05675"
     CDS             complement(3057..3515)
                     /locus_tag="VNY06_05675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX14332.1"
                     /translation="MSDNEPHGDFTVITEPTRNDHTQTLIETLKRASGARYSAIATVH
                     KECVIALSRLYVPHISPSPSPDQFRDMADFLVHWARVADRVLKIVGDEARANTTENIN
                     LGYFQGQFQATVEGYSSFELERCAANVEAEWEEFGGYDPDYQYEDRRAAE"
     gene            complement(3512..3748)
                     /locus_tag="VNY06_05680"
     CDS             complement(3512..3748)
                     /locus_tag="VNY06_05680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX14333.1"
                     /translation="MMTIATALRSRDPDKASAINTFAMAHNSDPMVVQARALGMELER
                     EQLKQKYENAMLVLAFSVTVNIGAIIYFLTRGLQ"
     gene            complement(3749..3991)
                     /locus_tag="VNY06_05685"
     CDS             complement(3749..3991)
                     /locus_tag="VNY06_05685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX14334.1"
                     /translation="MTSKTFKWTVEFEVAECWVADGFNLTDERALDMLSNDLGWTDIQ
                     TELKARVVKAPEPERIAKAQGYTDVSKFLADMQAGR"
     gene            4299..4514
                     /locus_tag="VNY06_05690"
     CDS             4299..4514
                     /locus_tag="VNY06_05690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013843972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdaS family helix-turn-helix protein"
                     /protein_id="HWX14335.1"
                     /translation="MDTETPKEALQEALRAIGGAAALARRLGIKQPSVFGWEQVPAGR
                     VLAVEAATGVRRERLRPDIYPPSEAAE"
     gene            4511..5245
                     /locus_tag="VNY06_05695"
     CDS             4511..5245
                     /locus_tag="VNY06_05695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HWX14336.1"
                     /translation="MTPSPRERLAQALDTIGDYAKLAHKAGVTPRDFLNAQTGRPCPT
                     VPFLRICVAIRHDPMPELPHIMPDKPSDFDFGFFSMALRLKRGLNGHDEAQAGAAAGL
                     SPVTISRIERGHELAIGIIICACKYMNIHIFSYLAVTDAPSISKKIKEAKPPPLRPSV
                     SRETSAPMEPSPLAILLKTRAKTQRQVDMLGRALERLEGKKDYADAFERHQRYLAALA
                     EQTLAIEAVRLAESREVKVFGSLGAG"
     gene            5284..5475
                     /locus_tag="VNY06_05700"
     CDS             5284..5475
                     /locus_tag="VNY06_05700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX14337.1"
                     /translation="MNKEERIDIAARAMWRHRAREPELSAWGKMSWEEFQIEKADIAQ
                     IYLDNARAAVTALDAAAVV"
     gene            5734..6198
                     /locus_tag="VNY06_05705"
     CDS             5734..6198
                     /locus_tag="VNY06_05705"
                     /inference="COORDINATES: protein motif:HMM:NF020715.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1937 family protein"
                     /protein_id="HWX14338.1"
                     /translation="MGEELRYLASPYTAYKGGMEQAFIDVAILAGRLFAIGIPTFSPI
                     AHAYPYVKYGNLDPFDYPAWKPIQERLMDACDSLIVAHMDGWESSVGIAHEVAYFERS
                     KKPIFDLNVETLVLIKRDPNKHFDERMSLGIKDMPSYRGTLGPLAMPDPKGQ"
     gene            6198..7409
                     /locus_tag="VNY06_05710"
     CDS             6198..7409
                     /locus_tag="VNY06_05710"
                     /inference="COORDINATES: protein motif:HMM:NF022744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3102 domain-containing protein"
                     /protein_id="HWX14339.1"
                     /translation="MNDRSTLIALRPPIGASAQVWASHITTCWRASFEGILETGHLLI
                     AAKEALEHGEFQKMIRSELPFKTNTAERLMAIARDERISNAANSPLLPPVWTTLYDLT
                     KLDDDKFESRIKDGTIRPDMVAKDISPDLGKKTADSRRKLAQELSDCAALQPTGRKFP
                     VIYADPAWRRKAGIGNRAYENHYTTEAWDEILAMPVVNRTLPDAWLFLWIPRAHLLAL
                     HPTLIETELGPCKVKLPLAYAVAQAWGFDAYSTCFVWTKTDEECPEDHGLGLIVWDQD
                     EILCLFKKGRGLPKPDTEVKVGSNHRERAGPHSEKPTYYRDMINAMTGNLPVLELFAR
                     EDDQHVLPANFYTWGNQSKNTAEVRITDESGTTHEPANVPEIILPGMVPTFEYIGAFV
                     DLTPAGKAVAS"
     gene            complement(7709..7846)
                     /locus_tag="VNY06_05715"
     CDS             complement(7709..7846)
                     /locus_tag="VNY06_05715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX14340.1"
                     /translation="MLWINRGAVYDGAEGELFFAAVPCCQHGVGEAEIHGDALSAVEE
                     A"
     gene            7975..8733
                     /locus_tag="VNY06_05720"
     CDS             7975..8733
                     /locus_tag="VNY06_05720"
                     /inference="COORDINATES: protein motif:HMM:NF018785.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1376 domain-containing protein"
                     /protein_id="HWX14341.1"
                     /translation="MSYLPYRPEHVNDAIANSEELTNEELGAFVRITWALWRAGGYLP
                     DDHKKLARFARAGSRWGSIAPAVMAKLTIAAGKISCPEILMTIFLVEQRRARAAKAAA
                     ARHGKPDPGLSSRNPLDSLKSNITVASTEHVLAASNQNQSKNLESKSFNAPRLATGPQ
                     ANQAFYELSVEFLIKRVGVRSLAARSQVAKWLAAVDGNEADLQAIMDGAGRENLNGAR
                     LIAVIDQRVGSRQAERKRGPPLPFPPNLVVNDGK"
BASE COUNT         1800 a         2498 c         2484 g         1969 t
ORIGIN      
        1 atcttgccgt aattgtctcc aagacgaagc ctctccacaa tatcgttgct tgttttcagg
       61 gacagccagc ctagcttgga gccgatttta tcgcggatgg gggagcccgg agccatcgcg
      121 agccgtcgaa cggcgctcat tttggcgcga acctcaggat tggccatcgc caatctgtgc
      181 cgcgcgcgca attccgggat gtgcattgcg ttcagcatgg agcggccact gcatcaaggt
      241 tccaaaagaa agagcgggcg cgatcctcgc ccatgattat gtcacgtacg atttcaccgg
      301 cggcgttttc gatggtgagt gttgggatgc caagttggtt cctttcgcca tcggcaaaga
      361 tggccacata gatcgcaccg tcaaggccgc tgtagacaac gggctttcct ttgatgaagc
      421 tttccatctc gtcctcatac aaaaaggcac taattccgta acactcacca atttccgccg
      481 atggtgtcgg ctgcaccgaa cgtgcagggg ccacacatgc cggtcagcgg caccactggc
      541 gattgtccgc agacctcaca ttgcccttcc caatccggct caagctcggg gtcgtcgcac
      601 tggaatttgc cggcctcttt gcggcccttg tttatctcgc gattgcgctt acccacggga
      661 atctccctgc atacttgttg ttactcgtct gacatgaatt tcagtttggg caacgttacc
      721 ttcggttttc gcaaagtttg tccctgctgt tgccggacct taagagaccg agtattgcct
      781 ttggtccgcc cacccaaagt ttttctctca tcgaacacgt cacgaatatt tgcgtggttc
      841 ctgacaggac caatttgtcg ctcgcaagcc ccggtgcgtt cgttcaacct caaacgatac
      901 cgggtcgcct tcaagaattg cgcctggtgt tgccggcagg tccgtgcgat gcacgaacac
      961 gtcgccgccg ccgtcatcgc gcgtgaaaaa gccatagcct ttttgctcgt tgaagaactt
     1021 aaccttgcct gtgaccgcca tgattggctc ctgttcaagc tacgaaagcg ttcctgctct
     1081 tgctgcgaat atcacgtcgc gcatcttaac caaatccccg cgcgctgcat agagtcgttc
     1141 gcaaactaaa agcgggtgta ttccgacgat ggaccagaat gcgcgctccc cgatccgatg
     1201 ctgttccttg tgacagtcgg tacacaaggg caaagcgaat ttatcatgcg gcttcttgcc
     1261 catgccgccg tgtttgttgt ggcgcccgat tgcatccgga cgtgggcggc ttcgcttggt
     1321 tcgtcattgc agccgaggca gggaagctgg cggattgatg cgaggtaggt aggatcggaa
     1381 tcggctacga ctttcaactt gatccgctgc gctatggcga aggcgcgttt taggagtgcg
     1441 cctgggggag cgggttgagt gaggcgttgg ggatggtggg gcttcatggt gttttgtttg
     1501 gttggactgg ctaccttggc tgtcacgtca ccgcgagggt tttaccgcca tagaggcttg
     1561 cgtcgtctag tcgtcgcgtc cataagtttt ctgatacact ccgcggagcg tattcaccgc
     1621 gtcgatcagc gaagcctcgt tcaactcgac gccgccgcga tcgatgatgt gcccgaggat
     1681 ggcgcaggtc cacattctct cactgtcctt cggcgccgtg ggacgatagt agccattctg
     1741 cggcgcctgc ggcttcgcct gtgtcaccgc tggcgtcaac gtcgcgccgt tgcgtcgctg
     1801 tgtcggttcg gtgatttcag tatactcgcc gcgcggctcg gcttgctgca cgatgcgctt
     1861 gacgccgatg atgttcttaa agccgttggt gccttcggtg taggcaagct cgtaagtgtt
     1921 gcccggctcg aattcgcgga tgcggtcttg tttgaccgga aaggtggtgt tgtccgcgcc
     1981 tttgatgaag ccgtattgct tgccttgctt cggcggtcca acatatgtga cggcgatggt
     2041 tgcggtttgc attggctcac aagctccgtt cgtaggttga tttgacaccc tctggcagtt
     2101 cgccagtagc ttcgcggaat cgctttgcgt ccttaataat ctgttttttg agatcgtcag
     2161 agcatcccat gatggtgatg gctgcacagg cgtcctccac ggtaaagact tcggtggtcc
     2221 gcatggagag tgacttgttc cccatcacgg aggcgatgcg aaccgagacg ttgcgttccg
     2281 ccgtagccgc ggctctgtcg gctttctgaa aacccttgaa cgcttggtcc gcgtcggcga
     2341 tggcgccgcc aacgtccgtg cattcacctt gctccgcgtt gtggatggcc tcttgctcgc
     2401 gcgcctcggc ttcgcgggcc tcacgttcct gccgctcggc ttcctcgcgc aaccgttggg
     2461 cttcgcgcaa tcgggcggct tcgacggcat tgttgaattt gctcagccgc gccttggcga
     2521 gttcgtagag tttttcgagc ggctggcgca ccaggcggta tggcttgttg atcgcttcga
     2581 gcttggcgtt gagtggatcg gcctgcggtt tgcgttcgtc ctccatcgcc tgcaaggcaa
     2641 cgcgcgtgcg ctctatataa gccgcatact gcttggcggc gtcgaaattt tcgatgaccg
     2701 gattgttctc ggtgaatacc gaaagctcgg ccatcgcttc cttggcgctt tcgattggcg
     2761 atggcggctt gttgtggccg atgggcgcgt gcatgatcgc ctcctagaaa caggtatcac
     2821 aggaacagtg cgggcgcttg ccgctgcggc atcggtcgct ggcgtcatgc ggaggatgaa
     2881 attcgttgat ggcctttcgc tcgcgatcac aataggcaca ctcgcccgga tcggccttgt
     2941 ggcggcgacg aacgccgcgc gccgcttcca cttttttctg ttcgtccatc gcactctcca
     3001 acggtcgtgt gaatttccgt gtcgccgagg ctccccgtga tcaggaatga cccaacctat
     3061 tccgccgccc ttcgatcctc atactgataa tcaggatcat agccgccgaa ttcctcccac
     3121 tccgcttcga cgttagcggc acacctttca agctcgaagc ttgaataccc ttcaacagtc
     3181 gcctgaaact gcccctgaaa atacccgaga ttgatattct cagtcgtatt ggctctggct
     3241 tcatccccga cgatcttcaa cacccgatcg gcaacccgcg cccaatggac gagaaagtcc
     3301 gccatgtccc ggaattgatc cggcgatggt gagggcgaga tatgcggcac gtagagcctg
     3361 gacagcgcta tgacgcattc cttgtgcacc gtggcgatgg cgctgtagcg ggcgccagag
     3421 gcgcgtttca aggtctcgat cagagtttgc gtgtgatcgt tgcgcgtcgg ctcggtgatg
     3481 acggtgaagt cgccgtgtgg ttcgttgtcg ctcattgtaa gccccttgtc agaaagtaga
     3541 tgatcgcgcc gatattgacg gtgacgctga atgccaagac cagcatcgca ttctcgtatt
     3601 tctgttttag ttgctcgcgc tccaactcca tccctaaagc tctggcctgc accaccatgg
     3661 ggtcggagtt gtgcgccatg gcgaaggtgt tgatcgcgga agctttgtct gggtcacgag
     3721 agcggagcgc ggttgcgatg gtcatcattc accgtcccgc ttgcatgtct gcaaggaatt
     3781 tggaaacgtc cgtgtagccc tgcgccttgg cgatccgttc cggttccggc gccttcacga
     3841 cgcgggcttt caattcggtt tggatgtcgg tccagcccaa gtcgttgctg agcatgtcga
     3901 gcgcgcgttc gtcggtgaga ttgaagccgt ccgcgaccca gcactccgca acctcgaatt
     3961 cgacggtcca cttgaaagtt ttgctggtca tgtcagtccc ctgtgttcga tgatcgataa
     4021 taggcatagc ctaagcctaa aggcaatccc tattattcat gcttaatcgt aatggctgat
     4081 atgcaataaa gcacggttgc cttgaaccgc gacttaggcg tagcctatag gggcaagcct
     4141 ttgacacgct cacccggagc atccggggtt cacgaggaaa caccatgtaa cggaccagag
     4201 ggcgcatagg gtgttggaaa gggcgtcttg ctgcccgcat ttgctagacc cgcgatcggc
     4261 cggccgatca ttgcgccctc acccgttccg cgaaggctat ggacacagaa acccccaagg
     4321 aagccctaca ggaagccctt cgcgcgatag gtggagcggc ggcattggcg cggcgcctcg
     4381 gcatcaagca accctctgtt tttggctggg aacaggtgcc cgccggccgc gtgctggccg
     4441 tggaggctgc taccggcgtg cgacgggagc ggctgcgccc cgacatttac ccgccctcgg
     4501 aagccgccga atgaccccct ccccacgcga gcgcctagcc caagccctcg ataccatcgg
     4561 ggattatgcc aaacttgccc ataaagcggg cgttacgcca cgggacttcc ttaatgccca
     4621 gacggggcgc ccctgtccta ccgtgccctt cctgcggatt tgcgtggcga ttcggcacga
     4681 tccgatgccg gaattgcccc atatcatgcc ggacaagccc tcagacttcg attttggctt
     4741 cttttccatg gctttgcgcc tcaaacgggg cctaaacggg cacgatgaag cccaggctgg
     4801 cgccgccgct ggtctttccc ctgtgacaat aagccgcata gagcggggcc acgagctggc
     4861 aatcggcatt atcatttgtg cttgtaagta tatgaatatc cacatttttt cttaccttgc
     4921 cgtgaccgat gctccctcaa tttcaaaaaa aataaaagag gccaaaccgc cccctctgcg
     4981 gccctctgtt tcacgtgaaa catcggcccc gatggagcct tccccgctag ctatcctact
     5041 gaaaacacgc gcaaaaacgc aacggcaagt ggatatgctc gggcgtgcac tggaacggct
     5101 agagggtaag aaggactatg cggacgcttt cgagaggcat cagcggtatt tggcggcgct
     5161 ggccgaacaa acgctggcga ttgaggcggt taggcttgcg gagagtaggg aagtgaaggt
     5221 gtttgggtca ttaggggctg ggtgagcggc tacgatctca cgttcaacca ttggatggag
     5281 aaaatgaaca aggaagaaag aatcgacatt gcggcgcggg cgatgtggcg ccatcgggcg
     5341 cgcgagccgg aattgagcgc atggggcaaa atgtcgtggg aggaattcca gattgagaaa
     5401 gccgacatcg cgcaaatcta tttggacaac gcccgcgcgg cagtcacggc gcttgatgct
     5461 gcggcggtcg tgtgaatttc cgtgtcgtct ccgtaacccg tgatccagaa tgacccaaca
     5521 cctttataag acggatcagc aaatcacggt attcgtgcta gacacgatac agacattcaa
     5581 cgccctatct ccgctacagc gcagagactt gcgcgactct caccgcaggc gttttgtaga
     5641 tgatagtctg acggcgcggc ggacttacat aaccgccaat tacgaacggg aaacgatgtt
     5701 cgataacctg gatttttcgt gagggggatg gaagtggggg aagaacttcg atatctcgca
     5761 agtccataca ccgcttacaa aggcggcatg gagcaagcct ttatcgacgt ggcgattctc
     5821 gccggccgct tgtttgcaat cgggattccg acattctctc ccatcgccca cgcctatccg
     5881 tatgtgaaat acggcaatct cgatccgttc gattatcccg cgtggaagcc gattcaagaa
     5941 cgcctgatgg atgcctgtga ttcgctgatc gtggcgcaca tggatggctg ggaatcgagc
     6001 gtcggcatcg cgcatgaagt ggcgtatttc gagcggagca aaaaaccgat ctttgatctc
     6061 aatgttgaaa cgcttgtcct gataaagcgc gatccgaaca aacatttcga cgaacgcatg
     6121 agcctcggaa tcaaagacat gccgagctat cgcggcacgt tggggccgct ggcgatgccg
     6181 gacccgaagg ggcagtgatg aacgaccgct ccaccttgat cgccctacgg ccgccaatcg
     6241 gcgccagtgc ccaagtttgg gcctcgcata ttacgacgtg ctggcgcgca agttttgagg
     6301 ggattttgga gactggtcac cttttgatcg cggcgaagga agcgttagaa cacggcgaat
     6361 tccagaagat gattcgctcg gaactgccgt tcaagaccaa tacggccgaa aggctgatgg
     6421 cgattgcgcg cgatgagcgc atttcaaatg cggcgaattc gccgcttttg ccgcctgtgt
     6481 ggaccacgct ttacgacttg acaaaacttg atgacgacaa attcgagagc cgaatcaaag
     6541 acggtacgat tcgcccggac atggtggcga aggatatttc gcccgatctg ggcaaaaaga
     6601 ctgctgattc gcggcgtaag ctagcgcagg aactatcgga ttgtgcggcg ctgcaaccga
     6661 ccggccgcaa gttccccgtg atctatgcgg accccgcctg gcgccgcaag gccggcatcg
     6721 gcaaccgcgc ctatgagaat cactacacga ccgaagcttg ggatgaaatt ctggcgatgc
     6781 cagtggtaaa tcgcacgctg ccggatgctt ggctttttct ttggataccg cgtgcccact
     6841 tgctggcgct gcatccgacg ttgatcgaga ccgagctagg cccatgcaag gtcaagctgc
     6901 cgttggccta tgcggtagcg caagcatggg gcttcgacgc ctattcgacg tgctttgtct
     6961 ggacgaaaac ggatgaagaa tgccctgagg atcacggcct cgggctgatt gtgtgggatc
     7021 aggacgaaat tctctgtctg tttaagaaag gccgcggcct gcccaagccc gataccgaag
     7081 tcaaagtcgg gtcgaatcac cgcgaacgcg ccgggccgca ttcggaaaag ccgacgtact
     7141 accgcgacat gataaacgcg atgactggca atctccccgt gctggaatta tttgcgcgcg
     7201 aggatgatca gcatgtcttg ccggcaaatt tctacacgtg gggcaaccaa tcgaagaaca
     7261 ccgcggaagt ccgaatcact gacgaatcag gaacgactca cgaacctgca aacgttcccg
     7321 aaataatcct tcccggcatg gtgccgacat tcgagtatat cggagcgttt gtggatttga
     7381 cgccggctgg gaaggcggta gcgtcttaag ttcttaaatg tgtttgttag cttggctacc
     7441 ttggctctca atcattctga gagattctta cggccataga ggattgagaa atgagccacg
     7501 cgagatttat gatgttgctc ggcgtcatgc tcggaacttg tctaggctcc gcattgatgt
     7561 ttctttttca acacactcaa tggccgtaaa accctaacaa caaaatcgac agccaaggta
     7621 gccagtctac ccagaatctt cttctaacaa acgcaagggg accaaggtga ccagaccaac
     7681 atatgcgctc tacgaatcag aagaacgcct aggcttcttc gaccgcagag agtgcatcgc
     7741 catgaatctc agcttcgccc acgccatgct ggcagcacgg gaccgcggcg aagaacaatt
     7801 caccctcggc cccgtcgtag accgctcccc gatttatcca caacattttc cgactcaacc
     7861 gcaacgctcg ctgatgtcgt ccagtgcagc gcaatgcctc gatacggcat gggatggaca
     7921 agcaccgacg cggctcacgc cattaccggg aaagcccacg tggggaggcc atcggtgagt
     7981 taccttccct accgccccga gcacgtcaac gacgccatcg ccaacagcga agaactgacg
     8041 aacgaggaac ttggcgcgtt cgtgcgaatc acttgggcgc tctggcgtgc cggcggttat
     8101 ctgccagacg atcacaagaa actcgcccga ttcgcccgcg ccggcagtcg ttggggatcg
     8161 atcgcacccg cggtcatggc caaactcacg atcgcggccg gtaaaatctc atgcccggaa
     8221 attctcatga ccattttttt ggtcgaacaa cgccgcgctc gtgctgccaa agccgccgcc
     8281 gccagacatg gcaaacctga ccccggcctg agttcgcgta acccattgga ctcgttgaag
     8341 tcgaacatta ccgtagcatc aaccgagcat gtgcttgcgg ccagcaatca aaatcaaagc
     8401 aagaatcttg aatctaagag ttttaatgcg ccccgcctcg ctacaggccc gcaagcaaac
     8461 caggcattct acgaattgag cgtggaattt ctcatcaagc gtgtcggagt gcggagcctc
     8521 gcagcgcgct cgcaggttgc aaagtggctt gccgctgtag atggaaatga agcggacttg
     8581 caggcaatta tggatggcgc cggcagggaa aacctcaatg gcgcccgatt gatagccgtc
     8641 atagatcagc gcgtcggtag ccgccaagcc gagcgcaaac gcgggccgcc ccttcccttt
     8701 ccaccgaatc ttgtggtaaa cgacggcaaa tgacccgata cgcctcagcc c
//
LOCUS       DATMPU010000249         2425 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_1142080,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000249 DATMPU010000000
VERSION     DATMPU010000249.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 2425)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2425)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2425
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1142080"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..132
                     /locus_tag="VNY06_05725"
     CDS             <1..132
                     /locus_tag="VNY06_05725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016847145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HWX14342.1"
                     /translation="DTIEKMQARIEHDLYYIDNWSFAFDLQILIRTVLSRKSYQNAY"
     gene            complement(157..420)
                     /locus_tag="VNY06_05730"
     CDS             complement(157..420)
                     /locus_tag="VNY06_05730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX14343.1"
                     /translation="MGQPNGMSVGETAGISGLMLAALCLGLFLARFNVFAVVVASILS
                     AVSLMLYLAQESTLAHPILLSLAIAFVVQIGYLVGQFPQKPRG"
     gene            818..1000
                     /locus_tag="VNY06_05735"
     CDS             818..1000
                     /locus_tag="VNY06_05735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018458801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX14344.1"
                     /translation="MKQPIRKESSTGLTGWRPFMIMGNAMPPRDPDDDEEEDEEDAEE
                     ENDPDEPPFVREPDED"
     gene            1034..1360
                     /locus_tag="VNY06_05740"
     CDS             1034..1360
                     /locus_tag="VNY06_05740"
                     /inference="COORDINATES: protein motif:HMM:NF019370.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GcrA family cell cycle regulator"
                     /protein_id="HWX14345.1"
                     /translation="MSWDEKDLALLKRLWAAGQSAAQIARRLACSRNAVCGRLTRLGL
                     KRGRKPPTAKPKIMLVPKRKPTSLAACARPVLKKVSGKKVAKQPKEFSKRQLYAMLAE
                     VVRNTD"
     gene            1699..1830
                     /locus_tag="VNY06_05745"
     CDS             1699..1830
                     /locus_tag="VNY06_05745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX14346.1"
                     /translation="MIELARASKSLDEAAKVTGRKPASIKKMAMRLGISLRPNAKKA"
     gene            2126..2308
                     /locus_tag="VNY06_05750"
     CDS             2126..2308
                     /locus_tag="VNY06_05750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX14347.1"
                     /translation="MFLRPPLARRGPWLVARIERAHHADARQHGVVAVRGYEQERLDR
                     GLSLRCLVPGRCPDRC"
BASE COUNT          562 a          689 c          722 g          452 t
ORIGIN      
        1 gacacgattg aaaaaatgca ggcccgcata gagcacgacc tgtattacat cgacaactgg
       61 tcctttgcct tcgacctgca aattctcatc aggacggtcc tttcgcggaa aagttaccag
      121 aacgcctatt gagacgccgg attcggcggt agcgccttat ccccgtggct tttgcggaaa
      181 ctggccgacg agatagccga tctgcacgac aaaagcgatg gcaaggctca acagaatggg
      241 gtgcgcgagc gtcgattcct gcgccaaata gagcatcaac gaaaccgcag aaagaatgct
      301 cgcgaccaca accgcaaaaa cgttgaagcg cgccagaaaa agccccaagc agagggcagc
      361 aagcattagc cccgatatcc cggccgtttc cccgacactc attccgttcg gctgccccat
      421 acgtctcagg aatcacctgg gatgatacaa tctcaggcgg gaggcgtttt gcaagaattt
      481 tggtcatcgt gctacccttg ctcagcgttt cacacgttgc ggcgggtccg atgactggca
      541 acgacgattt cacgtgcgcc cggacagcat ccccccgacg cgcgcgccaa agaccaaatc
      601 gcttctggcg caggtactca cgtccgcgca caaagcgggt agcatctcga gtggccgcgt
      661 caggagcagc ggcaggttcg gacgcggggc acgcacgacg actcatcacc gcttacgcgg
      721 accaccgccg gcctctccaa tatgcggtgc tgacttacat gcggaaacct gagcagaggg
      781 tataatccct aagaatccac acccgaggag cgacaagatg aagcaaccca tccgcaagga
      841 atcttccact gggttgaccg gctggagacc cttcatgatc atgggcaacg cgatgccgcc
      901 ccgagatcct gatgacgacg aggaggagga cgaggaagac gcagaagaag agaacgaccc
      961 cgacgaaccg ccatttgtgc gcgagccgga cgaggactag cccgcagctg gtagtcgcgt
     1021 tataatcttt cttatgagtt gggatgagaa agacctggcg ctgctcaaga gactctgggc
     1081 cgcaggacag agcgccgcac agatcgcgcg ccgtcttgcg tgcagccgca acgcggtctg
     1141 cggtaggcta actcgcttgg gcttgaagcg tggccgcaag ccgccaacag caaaacccaa
     1201 gataatgctg gtcccgaagc gaaagccgac ctcgttggcc gcctgtgccc gtccggtgtt
     1261 aaagaaggtg tcagggaaaa aagtcgcaaa gcagcctaag gaattcagta agcgccagct
     1321 ttacgccatg ctggctgagg tggtcagaaa tacggactga cggacttccg cgattttgca
     1381 acgggggcgc aacatagagc tgtgccaaat agagggtatc aggcgactcg atgacctgca
     1441 tccagccgac gtcccggcat tgagccttgc ggcagcgagc tctcccccat taacgttaca
     1501 cagttagctt aacgtgcggc atttgttgct aactttcgca aatcgaatat cgagggtgaa
     1561 ccggacaaat tcccaaagtc cggacccacc acggcgaagg cgtcaagcga tagttcgttt
     1621 ggcgcctttg cccggatcgc aaagctcttt gtttttatgg cccgaaaatt aggttggtcg
     1681 tttgcggaac agcgcctttt gattgaattg gcgcgcgcct cgaaatcctt ggacgaggcg
     1741 gcgaaagtga ccgggcggaa gccagcatcg atcaagaaga tggcgatgcg gctcggcatc
     1801 tcgttgaggc caaatgccaa aaaagcgtga ggcttcggct cgcggctggg acgcggtttc
     1861 cgtccattca tcaggcaaca ggcttcggtc ccgattggga ttgtaagatt cccaacagcg
     1921 agcccgtttg cataaagaag accagtcgac gaaaaatcga tggtgcgtgc agggcgcgtg
     1981 aacgaccgct ccgggtcaat aaccgtaagt ctcagattga gaacaatagg ccgcttggcc
     2041 cacggaagcg gacgttgacg gtgtcacttg attcgttggg acaagtgcca aaccgtgatc
     2101 gaggccattt ttatcgaacg atccggtgtt tcttcgcccg ccgcttgcgc ggcgtgggcc
     2161 ctggctcgtc gcgcggatag agcgcgcgca tcatgccgat gcgcggcagc atggtgtcgt
     2221 cgccgtgcgc ggctacgagc aggagcgcct tgatcgcggc ctgtcactgc ggtgcctcgt
     2281 gcccgggaga tgtcccgatc gctgttgaac tacgcgggcc gacgttgccg ggatgaatgt
     2341 tacgcggccc tcgtgacgtg ggcaacgggc ttgatcgtca cgcgatcgtg gtgagcttga
     2401 agggccgcac gcagaaccga caatt
//
LOCUS       DATMPU010000250         3194 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_1142085,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000250 DATMPU010000000
VERSION     DATMPU010000250.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 3194)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3194)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3194
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1142085"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            209..1340
                     /gene="prfB"
                     /locus_tag="VNY06_05755"
     CDS             join(209..277,279..1340)
                     /gene="prfB"
                     /locus_tag="VNY06_05755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012383943.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HWX14348.1"
                     /translation="MRAEAEALVQSIEQSLGLLRRHLDVDAATRRLAELNVKAEDPNL
                     WNDAAAAQKIMRERTELETRLGSLKRFENDLSDSATLVELGEAEQDEASENEGLAQLK
                     ALQLEAARLQIEALLSGEADANDAYIEVHAGAGGTESCDWARMLFRMYARWAERHKYK
                     VEIIEETPGDEAGLKSATMLVKGHNAFGWAKTESGVHRLVRISPFDSNARRHTSFASV
                     WVYPFVDDRVEIDVKESDCRIDTYRSSGAGGQHVNKTDSAVRITHIPTGIVVACQSER
                     SQHKNRSTAWNMLRARLYEQEMEKREAAADAVEAGKTDIGWGHQIRSYVLQPYQLVKD
                     LRTGTTSGTPSEVLDGELDNFMEAALAQKLSGGAPVVVEDVD"
     gene            1528..2337
                     /locus_tag="VNY06_05760"
     CDS             1528..2337
                     /locus_tag="VNY06_05760"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012383944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HWX14349.1"
                     /translation="MRRRREKIFNLPPVVLWTIALLFAIEGLCQLVSPEFYLGILQHF
                     AFVPGRFTFAFDPERVPLNFTGLPGDPEFRAEAAQFFLGDGKPVWWTVLTYAFLHGGW
                     LHVGINSIWFAAFGSAVARRFGAPRFLLFCAGAAVAGALAHYLTHMADLEPVVGASAA
                     VSGVMAAAVRFVFQPGAPLDEQLGFSDRVDEEFAYRMPALPLRNILSSRSAISFLLFW
                     FLANFLFGAFPAPAGIENAAVAWQAHIGGFLFGLLAFRWFDPPAPPHPIPA"
     gene            complement(2374..>3194)
                     /locus_tag="VNY06_05765"
     CDS             complement(2374..>3194)
                     /locus_tag="VNY06_05765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012589760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HWX14350.1"
                     /translation="APSLANALLIARAIGETLASSQKPLDILVTATASGLDVDVKGNG
                     AFDDQQRLALVRIALELDLARLSNHGEGLLTQRMPLIAIGKAVVAPPPGAFLQATEAG
                     EEILATRVCTQIAGVKRIADLFSGIGTFALRMAEFAAVSAFDLDERALSALAKAACPA
                     PLRPVALARRDLFRHPLTPKELENFDAAVFDPPRAGAESQARALAASSVPLIVGVSCN
                     AKTFARDAAILCAGGYELMPVEPIDQFRHTPHVEIIACFRRRASRPRRKRSVLG"
BASE COUNT          648 a          941 c          941 g          664 t
ORIGIN      
        1 aacaatctcc ggcaactacc tccgagagaa aaaaccgtat gatcacgctc cgacgggagc
       61 cgcggtcctt tccgaggccc aaaacgctct attcgcccgc gcgcgaattt cctatacgca
      121 ccagcatact tgcacccaga ccgccgtttg tttacagcag ctcccgacgt ttcttgtcaa
      181 ttatccttca ttcggaaagt gagcacccat gcgcgccgaa gctgaagcgc ttgtccaatc
      241 aatcgagcaa tcgctgggat tgctaaggag gcatctttga cgtcgatgcc gcgacccgcc
      301 gcctagccga gctcaatgta aaagccgagg atcccaatct ctggaatgac gccgcagccg
      361 cgcagaaaat catgcgcgag cgcaccgagc ttgaaacgag gcttggttcc ctaaaacgct
      421 tcgagaatga cctttcggat tcggccacgc ttgttgaact cggcgaggcg gaacaagacg
      481 aggcgtccga gaatgaggga ctcgcccagc tgaaagcttt gcaactcgaa gccgcacggt
      541 tgcagatcga ggcgctgctc tccggcgagg ccgatgccaa tgacgcctac atagaagttc
      601 acgccggcgc gggcggcaca gaaagctgcg actgggcgcg catgcttttt cgcatgtacg
      661 cgcgatgggc cgagcggcac aaatacaagg tcgagattat cgaggaaact cctggcgacg
      721 aagccgggct caaatccgcg accatgcttg tcaagggcca caacgccttt ggctgggcga
      781 agaccgaatc cggcgtccac cgtctggtac ggatctcgcc tttcgattcg aatgcgaggc
      841 gccacaccag ctttgcttcg gtttgggtct atccgttcgt cgacgatcga gttgaaatcg
      901 acgtcaagga aagcgactgc cgcatcgaca cctatcgttc gtctggagcc ggcggccagc
      961 atgtcaacaa gacagattcg gcggtgcgca tcacccatat cccgacaggg atcgtcgttg
     1021 cttgccaaag cgagcgctcg caacacaaga accgctcgac agcctggaat atgctgcgtg
     1081 cacggctcta tgagcaggag atggagaagc gggaagcggc ggccgatgcg gtggaagcag
     1141 gcaaaaccga tatcggctgg ggccaccaga ttcgctccta tgtgttgcag ccctatcagc
     1201 ttgtaaagga tttgcgtacc ggcacgacat caggaacgcc gagcgaggtt ctcgatggtg
     1261 aactcgacaa ctttatggaa gccgcactgg cgcaaaaact gtcgggtggc gcacctgtag
     1321 tggtcgagga tgtggactga acccgtactt tggcttaagc cttcaaaagc gttctcgtcc
     1381 atagcaaagc acaaatgctc caagttggta gcaaggcgca acgaatgcga cagtccggac
     1441 gcccgcggca ccttgtcctt gattggccat aacgccgctc gaggcaaaac cgttgccaga
     1501 ccggccggga tatgaatgcg atcactcatg cgtcgtcgac gagaaaagat ctttaatctg
     1561 ccgcccgttg tcctttggac cattgcgctt ctcttcgcga tcgagggcct ctgccagctc
     1621 gtctcgcccg aattctatct tgggatcctt cagcacttcg ccttcgtccc ggggcggttt
     1681 acctttgctt tcgatccgga gcgagtgccg ctgaatttta ctgggctccc tggggatccc
     1741 gagttcaggg cagaggccgc acaatttttc cttggtgatg gcaagccggt atggtggacg
     1801 gtgctcactt atgcgttttt gcatggcggc tggctgcacg tcgggatcaa ctccatttgg
     1861 tttgcagcct tcggctcggc ggtcgcccga cggttcgggg cgccgcgctt tttgctcttt
     1921 tgcgcgggtg cggcagttgc cggagcgctc gcgcattacc ttacgcatat ggctgatttg
     1981 gagccagtcg tcggggcttc ggcagcggtc tcgggcgtca tggcggcggc cgtgcgcttc
     2041 gtctttcaac cgggggcgcc gctcgacgag cagctcggct tttcggaccg ggtcgacgaa
     2101 gaattcgcat atcggatgcc cgcgcttcct ttgcgcaaca ttttgtcaag ccgcagcgcg
     2161 atcagttttc ttttgttctg gttcctcgcg aattttctct ttggcgcttt cccggccccg
     2221 gccggcattg aaaacgcagc tgttgcctgg caggctcata tcggcggctt tctcttcggg
     2281 cttttggcat tccgctggtt tgacccgcct gctcctcctc atcccattcc cgcctagcgg
     2341 ccggcacgcc atgctgtgga ttagtgtcgt acattaccca agaactgacc gcttccttcg
     2401 cggacgtgac gcacgccgcc tgaaacaagc aatgatttcg acatgcggcg tgtggcgaaa
     2461 ttggtctatt ggctcgaccg gcatgagttc atagccgccg gcgcaaagaa ttgcagcgtc
     2521 gcgcgcaaag gttttggcat tgcaggagac gccgacgatg agcggcaccg aggacgctgc
     2581 gagagcgcgg gcttggctct cggcccccgc gcggggcgga tcaaaaacgg cggcatcaaa
     2641 attttcgagc tccttcgggg ttagtgggtg acggaaaagg tcacgccggg cgagcgcgac
     2701 cggcctcaaa ggcgcggggc aagcggcttt tgccagggcc gaaagcgctc gctcatcaag
     2761 atcgaaagca ctgactgctg cgaactccgc catccggagc gcaaatgtcc cgatgccgga
     2821 aaatagatcc gcgatgcgct ttaccccggc gatctgcgtg cagactcttg tcgcaagaat
     2881 ttcctctcct gcctcagtcg cctgcaagaa ggctcccggc ggcggcgcta ccacggcctt
     2941 gccaatggca atgagcggca tgcgctgagt caaaaggcct tcgccatggt tcgagagacg
     3001 ggcgaggtca agctccaaag cgatacggac aagggctagc ctttgctgat catcgaacgc
     3061 cccattgcct ttgacatcaa catcaagacc ggatgctgtc gcggtgacga gaatatcaag
     3121 cggtttttga gagctcgcga gcgtttcgcc aatggcacgc gcaatcagca gggcgttcgc
     3181 cagcgaagga gcaa
//
LOCUS       DATMPU010000251         2907 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_1454491,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000251 DATMPU010000000
VERSION     DATMPU010000251.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 2907)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2907)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2907
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1454491"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..203
                     /locus_tag="VNY06_05770"
     CDS             <1..203
                     /locus_tag="VNY06_05770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019199312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX14351.1"
                     /translation="AERALVAHVGVFDVYEGAGIPEGKKSVAVSVTLQPREKTLTEAE
                     IDAAAGKIIADVTKKTGASLRG"
     gene            complement(751..972)
                     /locus_tag="VNY06_05775"
     CDS             complement(751..972)
                     /locus_tag="VNY06_05775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020176017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX14352.1"
                     /translation="MNKLERPREIIEYCLTPLALSTETAEYKEVYRRLEHVIKTLQVM
                     AARNNKPVAVDFSNMKTININETAHGDDA"
     gene            complement(1406..2758)
                     /gene="atzF"
                     /locus_tag="VNY06_05780"
                     /pseudo
     CDS             complement(1406..2758)
                     /gene="atzF"
                     /locus_tag="VNY06_05780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011662658.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004039 - allophanate hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="allophanate hydrolase"
BASE COUNT          677 a          813 c          821 g          596 t
ORIGIN      
        1 cggctgagcg cgcgctcgtc gctcatgtcg gcgtcttcga tgtctatgaa ggcgcgggaa
       61 tacccgaagg gaagaaatcg gttgccgttt ccgttacctt gcaaccgcgc gaaaaaacct
      121 tgaccgaggc cgagattgac gccgccgctg gcaaaatcat cgccgacgtc accaagaaaa
      181 ctggcgcaag cctcagagga tgaaaccttg agaaccattc tgcttgcctt tttcacggcc
      241 gcgttgatcg tggtttcggg tcaatcaaaa gccgctccgc gcaacattga cgactgcgag
      301 aaaattcagg cggcggatgc ttacaatctc tgcctcgcct cattcggtcc cgttgcccgc
      361 ggccatcacg cctttgcgga tggtgacgtt ggtggaaagc aagatgagac ggtgtcaagc
      421 gggtcccgcc acgtcgaggc cgcagcagcg gaacgatcgc ggcatggcca tcgccatgcg
      481 ttcaggcacg gatcgtcgcg gcatcgctgg gcaagccaca gctccggaca caggaccaga
      541 gcggtcttcc atcgcagtaa gcacgggcac aacacagcaa tggcattcag cgtcgtttaa
      601 ggctgcacgc gactgcgtca gtaattgcgc tggtcatcaa gagccacggc ggcgtggcgc
      661 tcgccagcaa tgatgcactt cctgtctgcg aatgccaagg ttttttgcgc cggcccgctg
      721 cagcctcggc ttggccgacg ccagtaacgc ttaagcgtcg tcaccgtgcg cggtctcgtt
      781 gatgttgatg gtcttcatat tggaaaagtc gaccgctaca ggcttgttat ttcgtgcggc
      841 catcacctgt aacgtcttaa tcacatgctc gagcctgcga tagacctctt tgtattccgc
      901 cgtctccgtc gataaagcaa gtggggtaag acaatactca atgatttcgc gaggccgctc
      961 caatttgttc attcgtatga ctcctttgag cacgaaacgg tggcgcctaa aatagttcga
     1021 agaacttata gtcccaaaat gagatttgac aaaaatctcg cgcaagcggc gaaggaaact
     1081 tcgccccata aatcggcgcc ttttaattca tgcaggagtt aggaagtcta ttgtgaacac
     1141 cgagtggcaa ttccccagca agggacgttg tccctttgtt cctcatttct gcgccgtcgt
     1201 cgtcgttgcg gctaagggcg tgttgaggaa ctgactttgt gtcaggaaag gcagcaaaac
     1261 taggatcaag agcaacatga cgttcggaga agactcatct tgctctagcc gcgctgtcag
     1321 aactccgcac gcaaagtgcc gttttcctga attgcctgca gcttaagtcc ttggcttaag
     1381 aaataccggc tttctcgcgg gcagtcccag cgccacaatt cgcggcctca tgcatagccg
     1441 ccgctggcaa ggcaagggcg tcatcgtgaa atggaggcgc aacgagagtg accccaaaag
     1501 gcaggccgtc cttgcgaaag ccagccggaa cagcaatcgc cgggcagctc aggaagttca
     1561 caaactgggt atagtggcca agccgggcat tgagctggac aggatcagcc agcatctctt
     1621 caaccttata gatagtgggt gcagtgggca gcagcaaaat atgcgctttg gcccattccg
     1681 cttcggcaat gcggcttaat tctttcaacc tatgcatgcc ggcaaaggca tctgccgcag
     1741 tatggcggtg cgcgctcgcc agcaacacac gcacggtctc attcatctcg tgtggatgag
     1801 catcaacaaa agccccaatc gagacgagcc gttcggcgag ccaaggccct tcatagagga
     1861 gcgctccgat ttctcgaaaa gggcggtaat cgaaggtaac aatctcggca ccgaggctgc
     1921 gcatgatttc gatggcctgc gcatagagcg cagcatattc ctcgtctccg ttgaattcgc
     1981 ggtcgtcttg cgaaagcacg ccgacgcgaa gctgcgcggg caatggtctt tgagattcgc
     2041 gcagcgagaa tgggtcggat ggatcgaatc cttcgaccgc gcggcgaacc gctattccgt
     2101 cagcaacact cgcggaaaag atactcacgc gatcaaggct gcggcaagct ggtagaacgc
     2161 ccaccatgct cagaaggcca cccgttggta taataccaac gatattattg aaagcagccg
     2221 gaacccggcc cgagcctgcc gtatcggtgc caagggcaaa tggcacaagt cccgaagcaa
     2281 cagcaactgc cgatcctgag cttgatccgc ctgatatata gctttcgttg aatacggagc
     2341 gcggcgcgcc atagggagat cgagtgccat tgagaccggt cgcgaactga tcgagatttg
     2401 ttttgccgac gataagcgca ccagcggcct taagccgcgc cacgaccatc gcgtcttttt
     2461 ccgcgcgata ggcgaaagct gggcaagccg ccgtggtatc gaggcctgcg acatcaatgt
     2521 tgtccttgac cgcgaaggga acaccccaaa gtggaaaaag tgcgggatct ggcttagtct
     2581 gcaacagtcg gtctgcgctg cgcctcaact ctgcgggatc aaggagcgag atgaatacgg
     2641 cggggtcatg agctgaacgg gtgcgcgcaa ttatctcttc tgcgacatca tgtatcgtgg
     2701 atggacgctc gccgtagagc gcgcgcagat ggccgaatgt tggagcccta tgaaacatat
     2761 tactgtccac actaatcagg tataaaaaac cgcaacatga aagctggggc gctggcgcga
     2821 gcccaaatcg cggcgctgca ctcgaaatta aaggaaagcc cctacgcagc gaaccgcttc
     2881 cttgccgtca tgtcgaagtg ctttgcg
//
LOCUS       DATMPU010000252         2259 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_4212857,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000252 DATMPU010000000
VERSION     DATMPU010000252.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 2259)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2259)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2259
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4212857"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..886)
                     /locus_tag="VNY06_05785"
     CDS             complement(<1..886)
                     /locus_tag="VNY06_05785"
                     /EC_number="6.6.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF014563.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobaltochelatase subunit CobN"
                     /protein_id="HWX14353.1"
                     /translation="MHLLRTETRLLEEAETAVDLAQTPAEILFLSFSDSDLTLLAAAA
                     QGRPQNAPSLRLANLSLLKHPYSVDLYVEKAARHARFVLVRLLGGLDYWRYGVEELSR
                     AAGDKNFALAIVPGDSMIDARLDAASSLPISDLRRIHQVFQEGGTEHLTPLFHFIASF
                     ANAKPTWREIPALKERPAAGRFEAGCRKRAWPRGQALILFYRSFMVAEDTEAILALAD
                     ALAAQEISVTTIFVSSLKDSDAIAFLRAELAEVKPDVIVNTTGFSARLTNESVLDASG
                     VAVLQAIFSGATEAQWKEN"
     gene            complement(890..1933)
                     /gene="cobW"
                     /locus_tag="VNY06_05790"
     CDS             complement(890..1933)
                     /gene="cobW"
                     /locus_tag="VNY06_05790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012386411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein CobW"
                     /protein_id="HWX14354.1"
                     /translation="MNSFSKIPATIVTGFLGAGKTTLIRHLLASAQGRRLALLINEFG
                     DIGIDAEILNGCGDLACGTDNIIELANGCICCTVANDFLPAIERLLALDPLPDHILIE
                     TSGLALPKPLVKAFDWPSIRARLTVDGMIAVVDGKAVAEGRFVDDPEQLARQRQADPS
                     IAHDNPLEEVFEDQMLCADLVVLNKADLISLPEQTRIAADIAATISRAVKILPAREGK
                     IDPAVLLGLYAAAEDNISARPSHHDGETDHDHDDFDSTVVEIGAVDDTDLLLRQLTEI
                     AKRHDVLRIKGFVEVRGKPARMLVQGVGKRFQKSFDRPWRVDEARRGRLVVIGQKGLD
                     KASIAKSLTNSWT"
     regulatory      complement(2053..2251)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
BASE COUNT          487 a          630 c          687 g          455 t
ORIGIN      
        1 ggttttcctt ccattgcgct tctgttgcgc cggagaagat cgcttgcagc actgccacac
       61 cagaggcgtc gaggacactc tcatttgtga ggcgggctga gaacccggtc gtattaacaa
      121 tcacatcggg tttcacttcg gcgagttctg cgcgcagaaa ggcgattgcg tcagagtcct
      181 tgaggcttga gacgaagatt gtggttacgc taatttcttg cgcggcgagc gcgtcagcga
      241 gcgccaaaat tgcctcggta tcttccgcga ccataaagga tcggtagaaa aggatgagtg
      301 cttggcctct gggccaagcg cgctttcggc agcccgcctc gaagcggcct gcagccggtc
      361 gctccttcag cgccggaatt tcccgccacg tgggtttggc gtttgcaaaa cttgcgatga
      421 aatggaaaag cggcgtgagg tgttccgtgc caccctcttg aaaaacttgg tgaatgcggc
      481 gaagatcact gataggaagt gacgaagcgg catcgagccg ggcatcgatc atcgaatcgc
      541 ccggcacaat ggcgagcgcg aaatttttgt cccccgcggc gcgggacaat tcctcgacgc
      601 catagcgcca atagtcaagc ccgccaagca gccggacaag cacgaagcgg gcatggcgcg
      661 cggccttctc gacatagaga tcgacagaat agggatgttt gagcaaggat agattggcga
      721 gccgcaggct cggggcattt tgaggccgcc cttgggcggc cgccgcgagg agggtcagat
      781 cgctgtcgga aaaggagaga aataagattt cggcgggagt ctgcgcgagg tcaaccgccg
      841 tctctgcttc ctcaagcaat cgcgtttctg tccgcagaag atgcatcgat caggtccagc
      901 tatttgtcaa agattttgca atcgaggctt tgtcgagacc tttctgacca atgacgacaa
      961 ggcgtcctcg gcgcgcctca tccactcgcc agggacggtc aaaacttttt tgaaagcgct
     1021 tgccgacgcc ttggacgagc atccgagcgg gcttgccgcg cacctcgaca aagcccttga
     1081 tccgcaggac gtcgtggcgc ttcgcgattt cggtcagctg acgcagcaag agatcggtgt
     1141 cgtcgacggc tccgatttca accacggtgc tgtcaaaatc gtcgtggtca tgatcggtct
     1201 ccccgtcatg gtgcgatggc cgcgcggaaa tattatcttc agcagcggcg taaaggccaa
     1261 gcaagactgc tgggtcgatc ttgccttcgc gcgcgggaag aatttttacc gcgcgggaaa
     1321 tggtcgcagc gatatccgcc gcgatgcggg tttgttcagg caaacttatg aggtcagcct
     1381 tgttcagcac gacgagatcg gcgcaaagca tttggtcttc gaacacttct tcaagcggat
     1441 tgtcatgcgc gatcgaagga tcggcttgcc gctggcgcgc caattgttct ggatcatcga
     1501 caaagcggcc ttccgccacg gccttaccat caacgaccgc aatcatgcca tcgacagtca
     1561 gccgcgcacg aatggaaggc cagtcgaaag cctttactaa gggttttggt aaggcgaggc
     1621 cggacgtttc aatgaggata tgatcgggca gcggatcaag cgccaaaagc ctttcgatgg
     1681 cgggcaaaaa atcattggca acggtgcagc agatgcagcc attggcgagt tcaatgatat
     1741 tatccgtgcc gcaggcaagg tccccgcagc cattcaagat ttccgcatcg atgccgatat
     1801 cgccaaattc gttgataagc agggcgagac ggcgtccctg cgcgcttgcc agaagatgac
     1861 gaatgagggt tgttttcccc gcgccaagaa agccggtgac aatggttgcc gggattttgg
     1921 aaaaggaatt catgaatggc accggctcct tgaacgcatg cgggtgccga aattgcggaa
     1981 gccggacgaa ccggctcctt ccgggagcgg tgaagcgcgc cggcatcgat gaaaagcccg
     2041 agcaccccga cgggccttct tcgatcgaac ggcaggtttc ctggctcgcg gatcatcgcc
     2101 tcgcgccgcc ttcccaggag agaagaaacc tatcccagtg gcttaaccgg cgcgcggctt
     2161 cccgctgaca gttgcggggg cagctgcggc tcgcggcgcc gagagggcac cctgccgcat
     2221 tcccatttac ccctcgaaga gagggaccgt tgcgcgtgc
//
LOCUS       DATMPU010000253         2781 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_3617601,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000253 DATMPU010000000
VERSION     DATMPU010000253.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 2781)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2781)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2781
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3617601"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            74..265
                     /locus_tag="VNY06_05795"
     CDS             74..265
                     /locus_tag="VNY06_05795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX14355.1"
                     /translation="MPFIIRWSNPAGTASTRRNTPIEARNWAVEMMGKGFQDVVILDE
                     NGKAYNPSDFAKLYVDTRT"
     gene            705..1130
                     /locus_tag="VNY06_05800"
     CDS             705..1130
                     /locus_tag="VNY06_05800"
                     /inference="COORDINATES: protein motif:HMM:NF012746.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HWX14356.1"
                     /translation="MQQIAEWLEKLGMSEYTELFSENKINISALRYLSDQDLKDIGIP
                     LGHRRIMQAAISQLASATPATPEPTPRIEPKPRDAAERRQITVMFCDLVGSTALSARM
                     DPEDLREVISAYQKCVAEIVQRFGGFVAKYMGDGCVGGY"
     gene            complement(1259..>2781)
                     /locus_tag="VNY06_05805"
     CDS             complement(1259..>2781)
                     /locus_tag="VNY06_05805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX14357.1"
                     /translation="DPTTIDLLSGIAERGALAPLFVVITARPEFRPPWGTRSHHTTIS
                     LAPLDRRQVRDMISELAARYALAKEMIDNVAERSGGVPLFVEEVTRLLLERGEQGGTQ
                     AIPPTLQQSLTARLDRLGPAREVAQIGAVVGRDFSYGLLHAVALMEDKPLQAALERLA
                     GADILLVQGLPPESDYRFKHALIQDAAYENLLKSRRQLLHRRVAEALRDNIAASAEPE
                     PELLAHHFNQAGMTEAAIEWWGKAGQRSLDRSALAEAVEQLTRALAQIETLPATSTLR
                     REQIKLQVALLTPLFILKGYGAPETKAAAERARLLIEQAEAHGETLEDPLLLFSVLYG
                     FWVAKLVAFNGDELRELAAQFLALAEKQGGAVPRMFGHRVVGTSLVHTGDIVQGRAHY
                     DQAIALYDPGTHLPLATRFGQDIRVATLCQRSLALWLLGYPEAALADAEQAVTHGREI
                     SQAATLMYALYFGRLTHFHCGIYEAVNAQLDELFALVDEQSCPSRFDRTLPVRQRY"
BASE COUNT          577 a          842 c          815 g          547 t
ORIGIN      
        1 cgattctctc cgtttgaatt ccgaaaaaca ttgacgctgc caaaaacggc ttcgacttgc
       61 tatcctcaat tgcatgccgt tcataatcag atggtcaaat ccggccggaa ccgcctccac
      121 tcgccgcaat actcccattg aagcgcgcaa ttgggctgtc gaaatgatgg gcaagggctt
      181 ccaagatgtc gtgatcttgg acgaaaacgg caaagcttat aatccatccg actttgccaa
      241 gctttacgta gatacgagaa cgtaatggca ctcgcgcgtc actacactga ggccgggctc
      301 atcgcctatt gaccggcggt gctgctcgcg gcgcaggtcc atcgtcggcg gcaatgccgc
      361 tagctgcggt gcatcaaggc gttcacaagc ccagcttggg attttatgtg ctcgcccagt
      421 ctctcagctc gctcgatcaa tgagtgcatc tgagcgagcg ctcagcgctg ttggaggccg
      481 caaagcaatt cacccgcgct gttgcctgcc aggtcgtgtc gcggacgacg aaaggtgctc
      541 ggcgtgcatg cgcgctttcg ctttcggcct cgacaccgcc ttcgtcatcg ctccacgaac
      601 agcggaatac cctctattat gcaaggctcg gcggcctgtt cacgtcatag cgccggtcgg
      661 gtatcgtggc gcggggctat aatcgtcgct gaggatgcga cgcaatgcag caaatagcgg
      721 aatggctcga aaaactcggc atgtccgaat acactgagct attttctgag aacaagatca
      781 atatttcggc tctccggtat ctgagcgatc aagacctgaa ggacatcggc atcccgctcg
      841 ggcatcggcg gataatgcag gcggcgatca gccagcttgc gagcgcaacc ccggcaacgc
      901 ctgaaccgac cccccgcata gaaccgaaac cgcgtgatgc cgccgagcga cgccagataa
      961 cggtcatgtt ttgcgatctg gtcggatcga cggcgctctc cgcccgtatg gacccagagg
     1021 acctgcgcga ggtgatctcg gcctatcaga aatgcgtcgc cgagattgtg caacggttcg
     1081 gcgggttcgt tgcgaagtac atgggcgacg gatgcgttgg tggatattag cgctaccgct
     1141 gagcagatca ttcagcgcta tcagaacgcg ccaaaaagct atatttttcg ggacgactcg
     1201 accggctggg ggcagagcga ccgcgacagc gctctccgcc tactgaagga ggccaacctc
     1261 aatatcgttg ccggaccgga agtgttcgat caaaacgact cggacatgac tgttcgtcca
     1321 ccaaagcgaa aagttcatct aattgtgcgt ttactgcctc gtaaattccg cagtgaaaat
     1381 gcgtcaatcg tccgaaatac agtgcgtaca tcaatgttgc ggcctgactg atctcgcgcc
     1441 catgcgtgac ggcctgctca gcgtcagcca gcgcggcttc gggatagcca agaagccata
     1501 gtgccagcga gcgctggcac aaggttgcaa ctcggatgtc ttggccaaac cgtgtcgcga
     1561 gcggaagatg cgtaccagga tcataaagcg cgatcgcctg atcgtagtgc gcccggcctt
     1621 gcacgatgtc tcccgtatgc accaaagacg tacccactac gcgatgcccg aacatgcgcg
     1681 gcactgcccc cccctgcttc tcggcaagcg ccaggaactg cgccgcaagt tcgcgcagtt
     1741 cgtcgccgtt gaatgctacg agcttcgcga cccagaagcc gtaaaggact gaaaacagca
     1801 acagcgggtc ttcgagagtt tctccatgcg cctccgcttg ttcgattagc agacgtgctc
     1861 gctccgcagc ggccttcgtt tccggagcac catatccttt gagaatgaag agtggggtta
     1921 gcagcgcgac ctggagcttg atctgctcac gacgcagcgt agacgtggcg ggcaatgtct
     1981 cgatctgagc aagagcgcgc gtgagttgtt ccacggcttc agccagcgcc gaccgatcca
     2041 atgaacgctg tcccgctttg ccccaccact cgatagccgc ctcggtcatg cctgcttggt
     2101 tgaagtgatg cgccagcaac tccggctcag gctcagcgct tgcggcgata ttatcgcgca
     2161 gcgcctcggc aacgcggcgg tgcaatagct gccgccggct cttgagtaaa ttctcgtaag
     2221 ccgcgtcctg gatcagcgcg tgcttgaagc gatagtcgga ttccggcggc aggccctgta
     2281 ccaacagaat gtcggccccg gcgagccgct ccagggccgc ttgcagcggc ttgtcctcca
     2341 tcagtgccac tgcgtgaagg aggccgtagg agaagtcgcg tccgaccacg gcaccgatct
     2401 gcgctacttc acgagccgga ccgagccggt cgagccgcgc tgtcagcgat tgctgcaacg
     2461 tcggcggaat cgcttgggtg ccaccctgct cgccgcgctc cagcagtagc cgggtcacct
     2521 cctcgacaaa cagcggcacc ccgccgctgc gctcggctac gttgtcgatc atttccttcg
     2581 cgagggcgta gcgagcggcc agttccgaaa tcatgtcgcg cacctgacgg cggtcgagcg
     2641 gcgcgagcga aatcgtagtg tgatgggagc gagtgcccca gggcggccga aattccggtc
     2701 gggcggtgat gacaacgaac aaaggcgcca atgcgccacg ttcggcgatg ccactaagaa
     2761 gatcgatggt tgtcgggtcg g
//
LOCUS       DATMPU010000254         2515 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_3919741,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000254 DATMPU010000000
VERSION     DATMPU010000254.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 2515)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2515)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2515
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3919741"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..258
                     /locus_tag="VNY06_05810"
     CDS             <1..258
                     /locus_tag="VNY06_05810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020050995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HWX14358.1"
                     /translation="RELARAKGTSVAQLAIAWVLAQGEDIVALAGARTRQRLTEALGA
                     PNVELSATDLAEIAWAVPADAAAGERYDTHQMRVLDSEKET"
     gene            complement(324..2192)
                     /locus_tag="VNY06_05815"
     CDS             complement(324..2192)
                     /locus_tag="VNY06_05815"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012591568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HWX14359.1"
                     /translation="MPRALAKKSLNIFRPKPKAAPKPKPAASLGDLPEWNLKDLYPAM
                     DSRAFADDLTKAASECRSFNVTYKGKLEGLANKASSAGLGLLQAIQRYEVLEELLGRI
                     MSYAGLVYAEDTTDPERTKFFGDTQEKVTNASSDLLFFELELNRLDDDLIARLAAGPL
                     KHYQPWIEDIRKGKPYQLDDKLEQLFHEKSITGYGAWNRLFDETIASLRFKVDGADLA
                     IEPVLNLMQDPDAKTREKAALAFGAGLAANLRIFALISNTLAKDKEISDRWRGFQDVA
                     DARNLANRVEREVVNALVDAVRTAYPRLSHRYYRLKAAWFDKTALKHWDRNAPLPRVP
                     TKTYQWEDARDIVLGAYDGFSPKMSGIARRFFDERWIDAPVRPGKQPGAFAHPAVPSV
                     HPYVLMNYQGKPRDVMTLAHELGHGVHQVLAGPNGALMAPTPLTLAETASVFGEMLTF
                     RALLLRTTNVAQRRAMLAAKVEDMLNTVVRQIAFYSFERKIHIERRSGELTAARICDL
                     WLTEQAESLGPAIEFGADYETYWAYIPHFVHSPFYVYAYAFGDCLVNSLYGVYQGARE
                     GFAELYIAMLAAGGTKHHSELLAPFGLDARDPAFWQIGLKMIEEMITDLEGMEGKA"
     gene            complement(2242..>2515)
                     /locus_tag="VNY06_05820"
     CDS             complement(2242..>2515)
                     /locus_tag="VNY06_05820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX14360.1"
                     /translation="IPASGCRSDGPKVALATLGLRKNRPKQIEEAIAAYREEEGTYEK
                     APGGWAVAKMNLGLALATLRRQMMAPTALSQPCLHQNIIPYIAPPN"
BASE COUNT          516 a          741 c          752 g          506 t
ORIGIN      
        1 cgcgagctcg cccgagcaaa aggaacgagc gttgctcaac ttgccatcgc ctgggtcctc
       61 gcgcagggcg aggatattgt ggcgctggcc ggcgcccgca cgcgccagcg gctcaccgag
      121 gcgctcgggg cacccaacgt cgaattgagc gccacggatt tggcagagat cgcgtgggcc
      181 gtgccggccg atgccgccgc aggcgagcgt tatgacaccc atcagatgcg cgttctcgac
      241 agcgaaaaag agacctgaca agtccgaaaa atgcggcacg gtgcgggcgc gaagagtttt
      301 gattcgcccc gccggatccc ggctcacgcc ttcccttcca tcccttcgag atcggtgatc
      361 atctcttcga tcatttttag cccgatttgc cagaaggcgg ggtcgcgcgc gtcaagccca
      421 aatggcgcga gaagctcgga atgatgtttg gttccgccgg ccgcaagcat ggcgatatag
      481 agctcggcaa aaccctcccg cgcgccctga tagacgccat agagcgaatt gaccaggcaa
      541 tcgccaaacg cataggcgta gacataaaac ggcgaatgaa caaaatgcgg aatataggcc
      601 caataggttt cgtagtcggc gccgaactcg attgcgggcc cgaggctctc tgcttgctcg
      661 gtcagccaca aatcgcaaat ccgcgccgct gtcagctcgc cgctacgccg ttcgatatgg
      721 atcttgcgct cgaaagaata aaacgcgatc tgccgcacca ccgtgttcag catatcctcg
      781 accttggcgg cgagcatggc ccggcgttgc gcgacgttcg tcgtgcgcaa caaaagcgcg
      841 cgaaaggtga gcatttcgcc aaagactgag gccgtttcgg cgagggtcag cggcgttggc
      901 gccatcagag cgccatttgg acccgccaaa acctgatgca cgccatgacc aagctcatgg
      961 gcgagcgtca tgacgtcgcg tggcttgccc tgatagttca tgagcacata aggatggacg
     1021 gaaggcaccg ccggatgagc gaaggcgcca ggctgcttac cgggccgcac gggcgcatcg
     1081 atccagcgct cgtcgaagaa gcgccgcgca atccccgaca ttttgggtga gaagccgtcg
     1141 taagcgccaa gcacaatgtc gcgcgcatct tcccattgat aggttttggt cggcacgcgc
     1201 ggcaacggcg cattgcggtc ccaatgtttg agcgcggtct tatcgaacca agcggctttc
     1261 agccgataat agcgatgcga gaggcgcgga taggcggtgc gcacggcatc gaccagcgca
     1321 ttgaccacct cgcgctcgac ccggttggct aggttccttg cgtcagccac gtcctgaaaa
     1381 ccgcgccagc ggtccgaaat ctccttgtcc tttgccaagg tattgctgat gagcgcgaaa
     1441 atgcgcaaat tcgccgcgag ccccgcgcca aaggcaaggg cagctttttc ccgtgtcttg
     1501 gcgtcggggt cctgcatcag attgagcacg ggctcgatgg caagatccgc gccatcaacc
     1561 ttaaagcgca ggctcgcgat ggtttcgtcg aacagccgat tccaagcacc atagccggtg
     1621 atcgatttct catggaacaa ttgttcgagc ttgtcatcga gctggtaggg cttgcccttt
     1681 cggatgtcct cgatccaagg ctggtagtgt ttgagcggcc ccgctgcgag ccttgcaata
     1741 aggtcgtcgt cgagccggtt caattcgagc tcaaagaaca aaaggtcgga ggaagcgttg
     1801 gtgacttttt cctgcgtgtc accaaaaaac ttcgtccgct ccgggtctgt cgtatcctcg
     1861 gcatagacga ggccggcata ggacatgatg cgccccaaaa gctcttccag cacctcataa
     1921 cgctggatcg cttggaggag ccccagcccc gcgcttgagg ctttattggc cagaccttcg
     1981 agcttgccct tataggtaac gttgaagctc ctgcattcag aagccgcctt ggtgagatca
     2041 tccgcgaagg cgcgcgaatc catcgccgga taaagatcct taagattcca ttccggcaaa
     2101 tcgccgaggc ttgccgcggg cttgggtttt ggggcggctt ttggtttggg gcgaaaaata
     2161 tttagggatt ttttagcaag cgcgcgcggc atggccctat cctatatgag atcgctgtca
     2221 taacgagcaa gcccccagcg atcaattggg gggagcgata tatgggatga tattttggtg
     2281 gaggcagggt tggctcaagg cggtgggcgc catcatctgc ctgcggagtg ttgcgagagc
     2341 aagaccaaga ttcatcttgg ccacggccca tccgcccggt gccttttcgt aggtcccttc
     2401 ttcctcgcga taggccgcga tcgcctcctc gatctgcttc ggccgattct ttctcagacc
     2461 gagcgtcgcg agtgcgactt tcggcccatc ggaacggcac ccgctcgcgg gtata
//
LOCUS       DATMPU010000255         9830 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_833848,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000255 DATMPU010000000
VERSION     DATMPU010000255.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 9830)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9830)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9830
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_833848"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            110..1504
                     /gene="tig"
                     /locus_tag="VNY06_05825"
     CDS             110..1504
                     /gene="tig"
                     /locus_tag="VNY06_05825"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012591897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HWX14361.1"
                     /translation="MEVTQIQAQGLKREFNVVLPAAEIAQRVEGQLAEIKAKANIPGF
                     RPGKVPISHLKRLYGRSIMAEVVQEAVNEANRKIVGENELRPATEPKIIFADDGRDLE
                     KVFEARFDFEFKVALEVLPKIEVHGLDDIEIERLVAEVSSSDIDQVLARLAETSRSFA
                     PKGGEAVAEAGDKVTLNFIGTIDGESFDGGSGQDVDVVLGSGGFLPGFEAQIEGMKSG
                     ERRTIAVTFPDNYTAAKLAGKLASFDVTVNSVAATGKIEIGDDLATGLGFEDLAKLKD
                     GIRANVERDYLAASRARWKRDLLDALDKKFLFEVPESMVSQEFDTIWRQVEAERSQNG
                     RSYEDNHTTEQAERADYQKIAERRVRLGLLLAEIGEGAKIKVTDEEVAQAVARRARAF
                     KGEDKAVWDNYRKNPRALAELRAPLFEEKVIDYIISQVKLTDKTVSKDELLNVAGSED
                     DRAKENRSLEAVQE"
     gene            1641..2291
                     /locus_tag="VNY06_05830"
     CDS             1641..2291
                     /locus_tag="VNY06_05830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018407650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HWX14362.1"
                     /translation="MRDRSPVEIYDNYLIPQVIENTSRGERGFDIYSRLLRERIIFLT
                     GPIEDHMASVIIAELLFLEAENPKKEISMYINTPGGVVTAGLAIYDTMQFIKPTVSTL
                     CVGQAASMGSLLLCAGDLNARYALPNARIMVHQPSGGFQGQASDIVRHAEDIMKVKHR
                     LNEIYVRHTGRDFETIEKTLDRDHFMSAEESKEFGIVDHVIVRRPDALPVPEGSRE"
     gene            2807..4072
                     /gene="clpX"
                     /locus_tag="VNY06_05835"
     CDS             2807..4072
                     /gene="clpX"
                     /locus_tag="VNY06_05835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020177230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HWX14363.1"
                     /translation="MTKVGGSDAKNTLYCSFCGKSQHEVRKLIAGPTVFICDECVELC
                     MDIIREENKSSLVKSRDGVPTPREICKVLDDYVIGQSQAKRVLSVAVHNHFKRLNHAA
                     KHNDVELAKSNILLIGPTGSGKTLLAQTLARILDVPFTMADATTLTEAGYVGEDVENI
                     ILKLLQASDYNVERAQRGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKIMEGTV
                     ASVPPQGGRKHPQQEFLQVDTTNILFICGGAFAGLEKIISARGKSTSIGFGATVQSPD
                     DRRTGEILRKVEPEDLLKFGLIPEFVGRLPVIATLDDLDEDALKKILTEPKNALVKQY
                     QRLFEMENTELTFQDEALNVVAKKAVERRTGARGLRSIMEGILLDTMFELPSLDSVEQ
                     VVIGPDVVDGKSRPLYIYSERSEKSGTSA"
     gene            4181..6598
                     /gene="lon"
                     /locus_tag="VNY06_05840"
     CDS             4181..6598
                     /gene="lon"
                     /locus_tag="VNY06_05840"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012591900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HWX14364.1"
                     /translation="MTEKREPARPGTIDTYPVLPLRDIVVFPHMIVPLFVGREKSIRA
                     LEEVMKADKLILLATQITATDDDPATDAIFATGTLASVLQLLKLPDGTVKVLVEGQVR
                     ANVVGYARTEEFYEARAEIIGDEAFVQVEVEALARSVVAEFEGYVKLNKKISPEVVAA
                     VMQIEDYSKLADTVASHLSVKIADKQAVLETISISKRLEKCLALMESEISVLQVEKRI
                     RSRVKRQMEKTQREYYLNEQMKAIQKELGDEDGKDDLAEIEDRIKTTKLSKEASDKAT
                     AELKKLRQMSPMSAEATVVRNYLDWILSIPWNKRSKIKKDLTVAENVLDSEHFGLEKV
                     KERIIEYLAVQSRANKLTGPILCLVGPPGVGKTSLGKSIAKATGREFVRMSLGGVRDE
                     AEIRGHRRTYIGSMPGKIIQSMRKAKSSNPLFLLDEIDKMGMDFRGDPSSALLEVLDP
                     EQNHCFNDHYLEVDYDLSNVMFVTTANTLNIPPPLMDRMEIIRIAGYTEDEKVEIARR
                     HLIPAGLKKHGLAPAEWSIDEGGLITLIQRYTREAGVRNLERELAKLERKAVKHILTG
                     KAKQIAVTSDNLPDFLGVPKFRYGEAEDEDQVGVVTGLAWTEVGGELLTIEGVMMPGK
                     GRMTVTGNLRDVMKESISAAASYVRSRAVDFGIEPPVFDRRDIHVHVPEGATPKDGPS
                     AGVAMATAIVSVMTGIPIRRDIAMTGEITLRGRILPIGGLKEKLLAALRGGLKKILIP
                     EENAKDLVEIPNSVKNALEIVPVSRMEEVLTRALVRQPIPIVWDEDAKPLAPLASEEE
                     PPGALAH"
     gene            complement(6665..7495)
                     /locus_tag="VNY06_05845"
     CDS             complement(6665..7495)
                     /locus_tag="VNY06_05845"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 family peptidase"
                     /protein_id="HWX14365.1"
                     /translation="MNKSSMCDYFRFFWRAIILLRGRNRGLRRLFLSLCGTALAAPAS
                     ALVGPAREAPQFAPYVVMVMYRSGGDTSFCTASVIMSNIVLTAAHCVSDLSDTRVFYR
                     GGLGKLALFDVATVAINPDYKPNAWRQHFVSIDLALLRLKEPLPSSFQPVELADFGPI
                     ATGQRLRIAGFGRADEDVSGTSGILRVGILSVTGPKSKLLAFLVDPDGFGLGGCTGDS
                     GAPIFAIDRPLLVAVAIRAKGKGGYSCGASTEAVQIGPQLPWIHEILRAWGTTRGMAQ
                     "
     gene            7530..7850
                     /locus_tag="VNY06_05850"
     CDS             7530..7850
                     /locus_tag="VNY06_05850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX14366.1"
                     /translation="MLLFIAVIASVYVSSSVFARAETQPDRVCFSTAETRDKIQVHGL
                     FEPFQLMRVTASRLHAEAMGVKLCRWHENLIYELSLLRHDGRIIYIFVNAQNGQPVGS
                     KNEN"
     gene            7919..8632
                     /locus_tag="VNY06_05855"
     CDS             7919..8632
                     /locus_tag="VNY06_05855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011472293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HWX14367.1"
                     /translation="MRLLVVEDDKDLNRQIATALSQAGYAVDRAFDGEEGWFLGDTEP
                     YDAVVLDLGLPKKDGISILSEWRRAGRSMPVLILTARDRWSDKVRGFDAGADDYVAKP
                     FHIEEILARLRALLRRAAGRATSEFTCGPVRLDARAGLVSVDGNPVKLTSHEYRLLSY
                     LMHHAGRIVSRSEIVEHLYEQDFDRDSNTVEVFVGRLRKKLGVDVIKTMRGLGYLLSA
                     PETAEAHAKPPGAGTPIPR"
     gene            8750..>9830
                     /locus_tag="VNY06_05860"
     CDS             8750..>9830
                     /locus_tag="VNY06_05860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012589479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HWX14368.1"
                     /translation="MRVGEEQDGNDSKPASSRLVAIRASPKPASMARRLFLSATFLSV
                     AILLISGIALSTLYRRAAESNFDERLGVYLRALVADIATPGESGPSAPGQLGEPHFEI
                     PLSGWYWQITRLDLQKPEIRNSRSLFAAQLPRLSDTGIAAGVGGARKGYVTGPDDRVL
                     RIVERIIDTGDQGIYLIQVAATTEEVEAEISNFERQLGLTFAALALALIISSALQLRY
                     GLKPLRKLQEGVAAIRRGEAEQIEGVFADDISPLASELNLLISANREVVEHARTQVGN
                     LAHVLKTPLSVIVNEFSADPSSPLAAKVEEQAAVMRDQLSFYLNRARAAVRARTVGSV
                     ADVTSAVEALVRTFEKIYAARAICFA"
BASE COUNT         2328 a         2608 c         2778 g         2116 t
ORIGIN      
        1 atttctaagc gtgatacagc ggccatgcct agggctattg cccgatcctg gcctcccggc
       61 aaaacttctg ccggtttgga cgccgcataa ttggtagagg catattcgaa tggaagtgac
      121 gcaaattcag gcccaaggcc ttaagcgtga attcaatgta gtgctgccgg ccgccgaaat
      181 tgcgcaacgg gtcgagggcc aactggccga gattaaggca aaggcgaata tcccgggatt
      241 ccgtccgggc aaggttccca tttctcacct aaagcgtctc tatggccgct ccatcatggc
      301 tgaagtcgtg caggaggccg tcaacgaggc caatcgcaaa attgtgggcg agaatgagct
      361 acggcccgct acggagccaa aaatcatttt tgcagacgat ggcagagatt tggagaaggt
      421 atttgaagcg cgatttgatt tcgagtttaa ggtcgcactc gaggtgttac caaagatcga
      481 agtccatggt ctcgacgaca tagaaatcga aaggcttgtc gccgaggtgt cctctagtga
      541 catcgatcag gtcttagcga ggctcgcaga gacgagccga agtttcgcgc cgaaaggagg
      601 cgaggctgtt gctgaagcgg gcgacaaggt gactttgaat tttatcggta cgatagatgg
      661 cgaatcgttt gacggcggct cagggcaaga cgtcgatgta gtgcttggtt ctgggggctt
      721 cctccctggc ttcgaagctc aaatcgaagg gatgaagtcg ggcgagcggc ggacgattgc
      781 cgtgaccttt ccggataact acaccgcggc caagctcgct ggcaaattgg cctcattcga
      841 cgtcaccgtc aactctgtag ccgcaaccgg taaaatcgaa attggcgatg acttagcgac
      901 gggccttggc ttcgaggatt tggctaagct caaggatggc atccgcgcca atgtcgagcg
      961 tgattatctc gctgcctcgc gcgcaagatg gaaacgtgat ctcctcgacg cactcgacaa
     1021 gaagttcctg ttcgaggtgc cggaaagcat ggtatcccaa gaattcgaca cgatatggcg
     1081 acaagttgag gcggagcgaa gccaaaacgg ccgcagctac gaggacaatc ataccaccga
     1141 gcaagcagag cgcgcagact atcaaaagat tgccgaacgg agagtacggc tcggcctcct
     1201 tttggcagaa ataggggaag gtgcgaaaat caaagtcaca gacgaagagg tcgcgcaggc
     1261 cgtagcgcga cgcgcgcgtg cgttcaaagg tgaagataag gccgtgtggg acaattaccg
     1321 caagaatccg cgggccctgg ccgagcttag ggccccactt ttcgaagaaa aggtcatcga
     1381 ttacataatt tcccaggtga aactgactga caagactgtt tcgaaagacg aacttttgaa
     1441 cgtggccggt agcgaagacg accgtgcaaa agaaaaccgc tcgctcgaag ccgtgcaaga
     1501 ataacgggtg tgttctccca atgccctggg caattgaatt ttcgcaggac catgcaatat
     1561 ggtatcattt gtaaattgtc tgaaaccatg attttcttgc cggttcgcag agaaggtttt
     1621 cacctcagaa aggcggctct atgcgcgatc gcagtccagt agaaatctac gacaactacc
     1681 ttattccgca ggttattgag aacacgagcc gcggcgaacg gggttttgac atctattcgc
     1741 gactgctgcg cgagcggatc atatttctga ctggcccgat cgaggaccac atggcttcgg
     1801 tgattattgc ggaactatta tttctggagg cggaaaaccc aaagaaggaa atctccatgt
     1861 atatcaatac gcctggcggg gtggtgacgg ctggcttggc gatctacgac acgatgcagt
     1921 tcataaagcc gacggtgtcg acgctttgtg tcgggcaagc ggcctccatg ggttctcttc
     1981 ttctatgcgc cggtgacctc aatgcgcgtt atgcactgcc gaacgcgcgt atcatggttc
     2041 atcaaccttc cggtggcttt cagggccaag cttccgatat tgttcgccac gccgaagaca
     2101 tcatgaaggt taaacaccgc ttaaacgaga tctacgttcg gcatacgggc cgggatttcg
     2161 aaaccatcga aaaaaccttg gaccgagacc atttcatgtc ggccgaagag tcgaaagaat
     2221 ttggtatcgt ggaccatgtt atcgtaagac ggccggacgc attgcctgtg ccagaaggca
     2281 gcagagagta agtagtctgt tgcttcttcc atgccggcga ccttcgtgaa ggagatttta
     2341 actgccggac cgggacccgc acgaggactt caggttgaaa tagccagcgt ggagcagttt
     2401 tgtttcgcca aggaccaagg cgattgtttt ggtatgacta ttgcttatat gttgcctcgc
     2461 gtgcagcgtt tgcgctatcc tggcaagcgg tatgtaacaa caaatcgaaa taaataccat
     2521 ttaggttaac ggaaaaattg cgtagatatg gtggaaatat cctggcacga ccaatcaagc
     2581 caactcggtc cggcgattga gggacttgtc ctgggcaagc gtgccgatcg tgcgttttca
     2641 aggagagtgt cagctcggtt agtgtggcgg tttcccactg ggaaccctag gcacgaacta
     2701 ggcaatgctt gatggtgtgt caatctaccg gcaaagggcg gtaagcctgc cgcctttaca
     2761 tatagcaccg cggttgttta aacttcagcg gggatggaga cgatcaatga ctaaggtcgg
     2821 cggtagcgac gcgaaaaaca cactctattg ctcgttctgc ggcaagagtc agcatgaggt
     2881 ccgcaagctc attgccggtc caacggtttt catctgcgac gaatgtgtcg aactttgcat
     2941 ggatattatc cgcgaagaga acaaatcttc gctcgttaaa agccgcgatg gcgtaccgac
     3001 ccccagggaa atttgcaagg ttcttgatga ctacgtaatt ggtcaatcgc aagcgaagcg
     3061 agtgttgtcg gtcgctgtcc acaatcactt caagcgtctc aaccacgcag ccaagcacaa
     3121 tgatgtggag cttgcgaaat cgaacatatt gttaattggc cctacggggt ccggcaagac
     3181 cttgttggca cagacgctgg cacgaattct cgatgtgcct ttcactatgg ccgatgccac
     3241 tacgcttacc gaggctggct atgtcggcga ggatgtggaa aacattattt tgaagctgct
     3301 tcaagcatcg gactacaatg ttgagcgagc acagcgcggg attgtctata ttgatgagat
     3361 cgacaagatt tcccgcaaat ctgataatcc gtcgattacc cgggacgtat caggcgaagg
     3421 cgtccagcag gcgcttctca aaatcatgga gggcactgtc gcatcggtgc cgccacaagg
     3481 tggccgcaag caccctcagc aggagtttct gcaggtcgat acgacgaata ttctgttcat
     3541 ttgcgggggc gccttcgctg gcctcgagaa gattatttcc gcgcgcggaa aaagcacatc
     3601 gatcggattt ggtgccacgg tccaatctcc ggacgaccgg aggacgggtg aaatcctgcg
     3661 caaagtagaa ccggaggatt tacttaaatt tggactgata ccggagtttg tcggtcggtt
     3721 gcctgtcatt gcgacgctcg acgaccttga tgaagacgca ttgaagaaaa tcctcaccga
     3781 accgaagaat gctttggtca agcaatatca gcgcttattt gaaatggaga atacggagct
     3841 aacgttccag gacgaggcat tgaacgtcgt tgctaagaag gccgtcgaaa ggcgtaccgg
     3901 ggcgcgcggt ttacgctcca tcatggaagg cattctgctg gacacgatgt tcgaacttcc
     3961 aagcctcgac agcgtcgagc aggtcgtcat tggccccgat gtggtggacg ggaaatctcg
     4021 gcccctttac atttattccg aacgcagcga aaaaagcgga acaagcgctt gagctgttca
     4081 accaatcgcc tgcggacaag aggagtgcct tcttgaaacg aggccgccgt atcgccattt
     4141 gttggacggc gcaccgcatt aatcccaaga ggacggcgaa atgactgaaa agcgcgagcc
     4201 cgctcgcccc ggtacgatcg acacatatcc ggtcttaccg ctgcgcgaca tcgtggtttt
     4261 cccccacatg attgtaccgc tcttcgtcgg tcgggaaaaa tcgattcggg ctctcgaaga
     4321 ggtcatgaag gccgacaagc tcattctcct tgcaacgcag ataacagcca ctgatgatga
     4381 tcctgcgacc gatgcaattt ttgcaacggg cacgcttgca tcagttcttc agctcctgaa
     4441 gctccccgat ggaacggtaa aggtccttgt tgaagggcaa gttcgcgcca atgtggttgg
     4501 atatgcacgc accgaggagt tttatgaggc gcgcgccgag attattggcg acgaagcctt
     4561 cgtccaggtc gaggtagagg cccttgctag gtcggtcgtc gctgagttcg agggatacgt
     4621 caagctcaac aagaagatct cgcctgaggt tgtcgccgcg gttatgcaaa tcgaagatta
     4681 ttccaagctt gcagacaccg ttgcgtccca tctttcggtc aagatagcag acaagcaagc
     4741 tgtccttgag accatttcaa tcagcaagag acttgagaaa tgcttggcgc tcatggagag
     4801 cgagatttct gttttgcaag tagagaagcg catccgtagc cgggtcaagc ggcaaatgga
     4861 gaaaacccag cgcgaatact atttaaacga gcagatgaaa gcgattcaaa aagagcttgg
     4921 cgacgaagat ggcaaggacg atctcgcaga gatcgaagat cgcatcaaga cgacaaaact
     4981 atccaaggaa gccagcgaca aggcgaccgc tgagttgaaa aagttgcgcc agatgtctcc
     5041 tatgtcggcc gaagcaactg tcgtccgcaa ctatctggat tggattttat caattccctg
     5101 gaacaagcgc tcgaagatca agaaggactt gactgtcgcg gaaaacgtcc tggacagcga
     5161 gcatttcggg cttgaaaaag tcaaggagcg tatcatcgaa tatctcgcgg tacaaagccg
     5221 tgcaaacaaa ctgacaggac cgattctgtg cctcgtcggg ccgcccggcg tcggcaagac
     5281 atcgcttggc aagtctatag ctaaggctac cggccgggaa ttcgttcgaa tgtcgctcgg
     5341 cggcgtccga gatgaagccg aaattcgggg gcaccgtcgc acctatatcg gttctatgcc
     5401 cggcaagatc attcagtcaa tgcgtaaagc taagagttca aatccgctat ttctgctcga
     5461 cgaaatcgat aagatgggga tggatttccg cggcgatcct tcctccgctc tgctggaggt
     5521 gctggatccg gagcagaatc attgtttcaa cgatcactat ctagaagtcg attatgattt
     5581 gtccaatgtg atgttcgtca cgactgccaa tacgctcaac attcctccgc cgctcatgga
     5641 tcgcatggaa atcatccgca tagctggcta cacggaagat gaaaaggttg aaatcgcccg
     5701 ccgtcattta atacctgcag gtctgaagaa gcacggctta gcgcccgcag aatggtcgat
     5761 cgatgagggc ggtctcatca cattgataca gcggtacacg cgcgaagccg gcgtccgaaa
     5821 tcttgaacgc gaactcgcta agctcgagcg gaaggcggtc aaacacatac ttaccggaaa
     5881 agcgaaacag atcgctgtca cgagcgacaa tttgccggat tttctgggtg taccaaagtt
     5941 tcgctacggc gaggcggaag acgaggatca ggtcggcgtt gtcactggct tggcatggac
     6001 cgaagttggc ggcgagctgc tcacgatcga aggcgtcatg atgccaggta aggggcgcat
     6061 gacggttact ggaaatctcc gcgacgtcat gaaagagtcc atttcggctg cagcgtccta
     6121 cgttcgctca cgagcggtcg actttggaat tgaaccaccg gtcttcgacc gacgagatat
     6181 tcatgtgcat gttccagaag gtgcaacccc caaggatggc ccctctgcag gtgttgccat
     6241 ggcgaccgcg atcgtttctg tcatgaccgg catcccaatt cgacgcgata tcgcaatgac
     6301 tggcgagatc acactccgtg gcaggattct gccgatcggc ggtctgaagg aaaaattgct
     6361 cgcggcgctg cgcgggggtt taaagaagat cctgattccc gaggaaaatg ccaaggatct
     6421 ggtcgaaatc ccgaattcgg tcaaaaacgc tctcgaaatc gttccagtct cgcgcatgga
     6481 agaagtgctg actcgtgccc tggtccgcca gccgattcct atcgtatggg atgaggacgc
     6541 aaagccgctc gctcctttgg cctcagagga agagccccct ggcgctcttg cccactagac
     6601 tagccgctcg atcccgcaag aattccgact gggctggcgc ccgcccgtac tggggcgtgg
     6661 gacattactg cgccatccct cgggtagtcc cccaagcgcg gagtatctca tgtatccatg
     6721 gcaactgcgg cccgatctgc accgcctcgg tagaagcgcc gcatgaatag ccgcctttcc
     6781 ccttcgctct gatcgccacc gcgacgagca gaggccgatc aatggcaaaa attggtgcgc
     6841 ccgaatctcc cgtgcacccg ccaagtccga acccgtcggg atcaacaagg aaggcaagga
     6901 gcttggactt aggcccagtc acactcagaa tacccacgcg caagatccct gacgttccgc
     6961 tcacatcttc gtccgctcgg ccaaaaccag ctatgcgcaa ccgctgtccg gtcgcgatcg
     7021 gtccaaaatc cgctaactca acgggctgga acgaggaagg caacggctct ttgaggcgca
     7081 acagcgccag gtcgattgaa acaaaatgct gccgccatgc gttgggcttg taatctggat
     7141 taattgcgac cgttgccaca tcgaatagag cgagttttcc caggccgccg cggtagaaca
     7201 cccgtgtatc ggaaaggtca ctaacacaat gggccgcagt aagtacgata ttgctcatga
     7261 tcacgctggc ggtgcaaaaa ctggtatctc cgccgcttcg atacatcacc atgaccacgt
     7321 agggcgcgaa ttggggtgcc tctcgtgcgg gtccgaccaa tgccgacgcc ggcgccgcga
     7381 gagccgtgcc acaaaggctt aggaataacc ttcgcaagcc gcgattacgg cccctcaaca
     7441 aaattattgc gcgccaaaag aaccgaaaat agtcgcacat tgaggacttg ttcatgggcc
     7501 attcatataa gcagcgccag ccttgaagca tgcttttatt tatagctgtg attgcgtcag
     7561 tctatgttag ctcgagcgtc tttgcgcgag cagaaacaca gccggaccgc gtctgctttt
     7621 caacagcaga gacgcgtgac aagatccagg tgcacgggct cttcgagccg tttcaactca
     7681 tgcgtgtgac tgcgagccgg ttgcacgccg aagccatggg tgtcaagctt tgccgctggc
     7741 acgaaaacct catctatgag ctgagcttat tgcgccatga cggtcgtatc atttatattt
     7801 ttgtcaatgc acaaaacggc cagcccgtcg gttcgaagaa tgaaaactag ggactgcaag
     7861 tgctgcggcc aagtggcatt tctcccaaat cggtgcatta atcgaggaat ttccaaacgt
     7921 gcgtttgctc gtcgtcgagg atgacaaaga cctcaatcgg caaattgcca ccgcgctctc
     7981 gcaggcggga tatgctgtcg atcgcgcctt tgatggtgaa gaggggtggt ttttgggcga
     8041 caccgaacct tacgacgctg tcgttctgga tttgggtcta ccgaaaaaag acggcatttc
     8101 gatactcagc gagtggcgca gggcaggacg ctcgatgccg gtactgattt taaccgcgcg
     8161 cgatcgatgg agcgacaagg ttcgggggtt tgacgctgga gcggacgact acgtcgccaa
     8221 gccattccac atcgaagaga ttctcgcacg gttgcgggcg cttttgcgcc gcgcagcggg
     8281 ccgcgccacg agcgagttca cctgcggtcc ggtacgtctt gacgcgcgcg cgggcctggt
     8341 ctcggttgat ggcaatccag taaagctgac ctcgcacgag taccggctct tgtcctattt
     8401 gatgcaccac gcgggccgga tcgtgtcgcg aagcgagatc gtagagcatc tctacgagca
     8461 ggattttgac cgcgattcga atacggtcga ggtattcgtg gggcgcctgc gcaagaagct
     8521 aggcgtcgac gtgatcaaga cgatgcgcgg actcggctac ctcttgtccg cgcccgaaac
     8581 ggctgaggca cacgctaagc caccgggggc tggaacacct atcccccgtt gaccttgcgc
     8641 ccgcaacacg ccgatcgctt tgatgccccg tcgagcgctg gtacgctgcg cctggctctg
     8701 gggaaacatt atgctagtga aggggcaaga tggagtgtag cggcagcaga tgcgagttgg
     8761 cgaagagcaa gacggcaacg acagtaagcc tgcctcgtcg aggctagtgg ctatccgtgc
     8821 ctctcccaaa ccggcttcga tggcgaggcg gcttttcctc tcagccacat ttctgagtgt
     8881 cgccatactt ctgatatccg ggattgcact ttcgacactc taccgccgcg cggcagaatc
     8941 aaatttcgac gaacgcctcg gcgtttactt gcgcgctctc gtcgctgata tcgcgacgcc
     9001 cggagaatct ggcccttcgg cgcccgggca gctcggcgag ccgcattttg aaatccccct
     9061 gtcggggtgg tattggcaga tcacgcgctt ggacctccag aagcccgaga tcagaaattc
     9121 gcgctcccta ttcgcggctc agcttccgcg cctttccgat acaggcatcg cagccggagt
     9181 cggcggtgcg cgtaaaggct atgtgaccgg gccggatgac agggttctac ggattgtcga
     9241 gcgcatcatc gataccggag atcagggaat ttaccttatt caagttgccg cgacgaccga
     9301 agaggtcgaa gctgagatct ctaactttga acggcagctc ggcctcacct tcgcagccct
     9361 cgcgcttgcc ctcatcattt cttcggctct gcagctacgc tacggcttaa aacctctgcg
     9421 taagctccaa gaaggggttg ccgccataag gcggggcgaa gctgaacaga tcgaaggcgt
     9481 attcgcagat gatatcagtc ctctagccag tgaacttaat cttttgatat cagccaatcg
     9541 ggaggtcgtc gagcacgcgc gcacccaggt tggcaacctt gcccatgtct tgaagacgcc
     9601 gcttagcgtc atagtcaacg aattctcggc cgatccttcc agcccactcg ctgccaaggt
     9661 agaggagcag gctgcggtta tgcgagacca gctttccttt tacctaaatc gcgcccgtgc
     9721 cgcggttcgc gcccggactg tcggcagcgt tgccgacgtt acctcggccg tagaagcttt
     9781 ggtccgtacg ttcgaaaaaa tctacgcggc acgcgccatt tgctttgcca
//
LOCUS       DATMPU010000256         2409 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_833996,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000256 DATMPU010000000
VERSION     DATMPU010000256.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 2409)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2409)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2409
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_833996"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(250..1107)
                     /gene="sseA"
                     /locus_tag="VNY06_05865"
     CDS             complement(250..1107)
                     /gene="sseA"
                     /locus_tag="VNY06_05865"
                     /EC_number="2.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003611459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004792 - thiosulfate sulfurtransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-mercaptopyruvate sulfurtransferase"
                     /protein_id="HWX14369.1"
                     /translation="MTKIVDEEYFVSTAWLAEILGEPDVAVIDGTFCLPDENRDAKAE
                     YVASHIPGAVFFDIDEIADHGTNLPHMLPQPAEFAHAMEKLGLSDQMRFVIYDSTNLQ
                     GGARVWWTLRVFGAKDVKLLAGGLPRWRAEGRPLEQGIVRRARSRFTVRFDKDAVASA
                     ELVKQASEGLSAQIIDARAAARFHGTTAEPRPLLRSGHIPGSLNLPWREVVKWGEIKP
                     REEVENLFAQAGLDFNRPVIATCGSGVTAAILLLALASVGKHDVALYDGSWAEWGARP
                     DLPVTKSTR"
     gene            complement(1203..2234)
                     /locus_tag="VNY06_05870"
     CDS             complement(1203..2234)
                     /locus_tag="VNY06_05870"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020176197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HWX14370.1"
                     /translation="MRFSKNVIEAIGKTPLIKLRRASEMTSCIILGKAEFMNPGGSVK
                     DRAALAIVEDAVARGILRPGGRIVEGTAGNTGIGLALVANALGYKTVIVIPDTQSQEK
                     KDFLRLQGAELIEVPAVAYSDPNNYVKLSGRLAERLSKEYPEGAIWANQFDNIANRKG
                     HFETTGPEIWAQTEGKIDGFTCAVGTGGTLAGVGMALKAKNPNVKIAIADPLGAALYA
                     YYTSGELKSFGSSITEGIGQGRITANLEDAPIDLAYQITDEEALPILFDLAEHEGLLL
                     GGSSGINVAGAIRLARDLGAGHTIVTILADSGARYQSKLFNPAFLRAKNLPVPAWMER
                     ELSIVPDFV"
BASE COUNT          527 a          728 c          643 g          511 t
ORIGIN      
        1 tcgcaaggcc aaccggctcg atccaagaga aattcgctgg cgcgtgccgc gttggaccga
       61 acccatccgg cgaccgccgg atgtccactt tcgcatcgaa cgcttgaccg tattaaggtt
      121 gcaagctctt catgcttgca accatgctac cgcacccgca tgccgcgtca agccttttta
      181 ctgcgccggt agacttttgg tgttggcaaa aggtggatct cgccgctttg ctctgcaaca
      241 tgaccaggct taacgcgtgc ttttagtcac tggcaggtca ggccttgcac cccattcggc
      301 ccaggagccg tcgtaaagcg cgacatcgtg cttgcccacc gacgccagcg caagcaagag
      361 aatcgcggcc gtgacgcctg agccacaagt cgcgatgaca ggccgattga aatccagccc
      421 cgcctgcgca aaaaggtttt caacttcctc cctaggcttg atctcccccc atttgaccac
      481 ctcgcgccat ggcagattga ggctgccggg aatatggccc gagcgcagca aaggacgcgg
      541 ctcggctgtc gtgccgtgaa agcgcgcggc ggctcttgca tcgatgattt gcgctgacaa
      601 gccttcgctc gcttgcttga caagttcggc acttgcaacc gcatctttgt cgaaacgcac
      661 tgtgaaccga ctacgggcgc gccgcacaat tccctgttcg agcggccgcc cttcggcccg
      721 ccagcgcggc agaccgccag ctaggagttt tacgtccttc gcgccaaaga cccgcaaggt
      781 ccaccacacg cgcgcaccac cttgcagatt cgtcgaatca taaatgacga agcgcatctg
      841 atcgctgaga ccgagctttt ccatcgcatg cgcaaactcg gcaggctgcg gcagcatatg
      901 tggcaaattg gttccgtgat cggcgatttc gtcaatgtcg aaaaagaccg ccccaggaat
      961 atgcgacgcc acatattccg ccttcgcatc ccggttttcg tccgggaggc aaaaggtccc
     1021 atcaatgacg gcgacatccg gctcgccgag aatttccgca agccacgcag tggacacgaa
     1081 gtattcttcg tctacgattt ttgtcattcc ggtgcctcat agtcgaaggg cgcgtcccgt
     1141 cgctgatttt tcatcgagcc tcggaccgcc aaatttcagc agagattctt gtcgctgtct
     1201 attcaaacaa agtctggaac aatggaaagc tcacgctcca tccatgcagg aacgggaagg
     1261 tttttcgcgc gcagaaatgc tgggttaaac agcttcgact gataacgcgc gccagaatcc
     1321 gctaagattg tcacgatcgt gtggcccgcg ccgaggtccc tcgccaggcg gatcgcgcca
     1381 gccacattga tgccagacga gccaccaagc agcagccctt catgctcggc aagatcgaac
     1441 aagatcggca aggcttcctc atcggtgatc tgataagcaa gatcaatagg cgcatcctcg
     1501 agattggctg tgatccggcc ctgacctatg ccttccgtta tcgaagagcc aaaggatttc
     1561 agctcaccgc ttgtgtaata ggcgtaaagc gctgcgccca gagggtcagc aatcgcgatc
     1621 ttgacattgg ggttcttggc tttcagcgcc atgccgacgc cggcgagcgt tcccccagtc
     1681 ccgaccgcgc aggtaaaacc gtcgatcttg ccttcggttt gcgcccaaat ctcaggcccg
     1741 gtcgtctcaa aatggccctt acgattggca atgttgtcga attgattggc ccaaatcgcg
     1801 ccttctgggt attctttgga cagacgctct gcgaggcggc cggaaagttt cacatagttg
     1861 ttgggatcgg aataagcgac ggccggaacc tcgatcagct ccgcgccttg cagtcgcaag
     1921 aaatctttct tctcctggct ctgcgtgtcg ggaatgacga tcaccgtctt atagcccagc
     1981 gcattggcga cgagcgcaag gccgatcccg gtgttgcctg cggtgccctc gacgatccgt
     2041 ccgcccggcc gcaaaattcc gcgcgcaacc gcgtcctcca cgatggccag cgccgcgcgg
     2101 tccttcacgg aaccacccgg attcatgaac tccgctttgc ccaaaataat gcagctggtc
     2161 atttccgatg cacgacgcag cttaatgagt ggggttttgc cgatcgcttc gatgacattt
     2221 ttggaaaagc gcatttgata cttgatccac accattatga ttaagagata gagctcatct
     2281 atgatgaaga tatagagctc atttaaaagg tctgtagagc ccgagcaagc gcctgctagc
     2341 acggagaaga cctttcacgc gtgaatgagc caatgagccg aaggagccga aggagccgaa
     2401 gggaaaatt
//
LOCUS       DATMPU010000257         7304 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_2077024,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000257 DATMPU010000000
VERSION     DATMPU010000257.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 7304)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7304)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7304
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2077024"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(51..689)
                     /locus_tag="VNY06_05875"
     CDS             complement(51..689)
                     /locus_tag="VNY06_05875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019400046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Maf-like protein"
                     /protein_id="HWX14371.1"
                     /translation="MTRDDRVWRPRLILASASPRRLELLQQIGIEPDALLAVDLDETP
                     RKLELPRNLASRLAAEKTRAAARIASSRPELAGAYIIGADTVVSVGRRILPKCEIVSE
                     AAQSLRILSGRAHRIHTAICLVTPNGRVRQKLVETRLRFKRLSAGEIEAYLGSGEWRG
                     KAGGYAIQGLAGGFAQKLVGSYSAVVGLPLYETLSLLVGEGFPVHVSWLNRV"
     gene            complement(717..935)
                     /gene="infA"
                     /locus_tag="VNY06_05880"
     CDS             complement(717..935)
                     /gene="infA"
                     /locus_tag="VNY06_05880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HWX14372.1"
                     /translation="MSKEELLEFPGTVIELLPNATFRVKLENEHEIIAHTAGKMRKNR
                     IRVLTGDKVLVEMTPYDLTKGRITYRFK"
     gene            complement(1132..1947)
                     /gene="pssA"
                     /locus_tag="VNY06_05885"
     CDS             complement(1132..1947)
                     /gene="pssA"
                     /locus_tag="VNY06_05885"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003610951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003882 - CDP-diacylglycerol-serine
                     O-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HWX14373.1"
                     /translation="MQRRKRGLRAVPMRIILPNLVTLLALSMGLTAIRFAVDGQYETA
                     VVAVIGAAILDGLDGRLARALRGTSRFGAELDSLADFVDFGVAPALILYFWGLHEIKS
                     FGWYAALVFAIAGALRLARFNVMIDDPNRPAWHAHFFTGMPAPAGAIVGLLPVYLNLS
                     ILAVPNTRSVAPFEIAYVLLIALLMASPIPHFSGKNVGRVPREYVLAFLFGIVACLLL
                     LATFTMEMLIGLSLIYLGTIPFAMRRFKAYVAADAARQASATPVPGPALQVRK"
     gene            2322..2576
                     /locus_tag="VNY06_05890"
     CDS             2322..2576
                     /locus_tag="VNY06_05890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX14374.1"
                     /translation="MADVEEIEQALSWAIKKRMLEGCGTLSKVSMIDGIVQVTFNGVT
                     DDTHVFDTLSGALLDLRLGLDIVPDLDVKIRRKWRLMPQR"
     gene            2802..6125
                     /gene="carB"
                     /locus_tag="VNY06_05895"
     CDS             2802..6125
                     /gene="carB"
                     /locus_tag="VNY06_05895"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012589714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HWX14375.1"
                     /translation="MPKRTDISTILIIGAGPIIIGQACEFDYSGTQACKALREEGYRI
                     VLVNSNPATIMTDPDMADRTYLEPITPEIVAKIIAKERYVVPGGFALLPTMGGQTALN
                     CALSLRKMGVLEKFWVEMIGASAAAIDMAEDRKLFREAMTRLGLATPRSHQIKTLPQA
                     LAVLDDIGLPAIIRPSFTMGGTGGGIAYNKAEYIDIAERGIDASPTNEILVEESVLGW
                     KEFEMEVVRDKADNCIIVCSIENIDPMGVHTGDSITVAPALTLTDKEYQTMRDAAIAV
                     LREIGVETGGSNVQFAVNPKDGRMVVIEMNPRVSRSSALASKATGFPIAKVAAKLAIG
                     FTLDEIANDITGGATPASFEPSIDYVVTKIPRFAFEKFPGAEQILTTAMKSVGETMAI
                     GRTFAESLQKGLRSLDTGLDGLNEIELEGLGLGDDMNVLRAALGIPTPDRLLVVAQAL
                     RHGMPQDEIVEACGIDPWFIERIAEILDLEARVRAHGLPADATNLRKLKAAGFSDARL
                     ANLAGAPEAKVSALRKKLGVRPVYKRIDTCAAEFSSPTAYMYSTYAPPFGSVPACESR
                     PSTRKKIVVLGGGPNRIGQGIEFDYCCCHASFALREAGYETIMINCNPETVSTDYDTS
                     DRLYFEPLTAEDVLAILANEGETGELKGLIVQFGGQTPLKLAHPLEQAGIPILGTSVD
                     SIDLAEDRDRFKRLLDKVGLKQPMNGIAYSVEQSRLVASDLGLPLVVRPSYVLGGRAM
                     AIIFDNAAFDDYLLGTLPGLVPSEVKARYPNDKTGQINTVLGKNPLLFDRYLSDATEI
                     DVDALSDGKDVFICGVMEHIEEAGIHSGDSACSLPPRSLSRQTISELEYSTKKLALAL
                     GVGGLMNVQYALKGGEIYVLEVNPRASRTVPFVAKVVGLPVAKIAARIMAGDSLASFG
                     LRPREFDHIGVKESVFPFARFPGVDTVLGPEMRSTGEVVGLDRSFPIAFAKSQIGGGT
                     NVPRTGTVFVSVRDIDKPRVVETARLLTSLGFKVRATGGTARYLESAGVEALRVNKVS
                     EGRPHVVDLIKNGDIQLMLNTTEGAQALADSRSLRRAALLHKVPYYTTLAGAIAAAQG
                     IRAYLAGDLEVRALQDYFA"
     gene            6515..6988
                     /gene="greA"
                     /locus_tag="VNY06_05900"
     CDS             6515..6988
                     /gene="greA"
                     /locus_tag="VNY06_05900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012383875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HWX14376.1"
                     /translation="MDKIPMTAGGYSTLEEELKRRQQIERPRIIQTIAEARAHGDLSE
                     NAEYHSAKEAQALNEMRIAELEDKLSRAEVIDISKLSGNTIKFGATVTLVDEDTDEKR
                     VFQIVGENEADVKAGRVSITSPTARALIGKKTGDSVEVNTPGGGKSYEILDVAFQ"
BASE COUNT         1669 a         1987 c         2099 g         1549 t
ORIGIN      
        1 agaggagtgg cggccatggg tttggctata gctgtgctag ggaagcaata ctatactcta
       61 ttcagccaag agacatgcac cggaaaaccc tcccccacca gcagcgataa ggtctcatag
      121 agagggaggc cgaccacagc cgaataggat ccgacaagtt tttgcgcaaa tcctcccgca
      181 aggccttgaa tcgcatagcc tccggccttg ccccgccatt cccccgatcc gagataggct
      241 tcgatctccc cggccgaaag ccgtttgaaa cgcagcctcg tttcgacaag tttttgacgc
      301 acccgcccgt tgggcgtgac caggcaaatg gccgtatgaa tccggtgcgc gcgccccgac
      361 aaaatccgta aggattgcgc cgcctcagac acgatctcac atttaggcag aatgcggcgg
      421 ccgacgctaa cgactgtatc ggctccaata atataggcac ccgcaagttc tggccggctc
      481 gaagcgatcc gcgcggcggc tcgcgttttt tccgcggcca agcgcgatgc gagattgcgc
      541 ggcagctcaa gcttgcgcgg ggtttcgtcg agatcgaccg caagaagagc gtccggctcg
      601 atgccaatct gttgcaggag ttcgagccgc ctaggtgacg ccgaagccaa aattaggcgt
      661 ggccgccaaa ccctatcgtc acgggtcatg gcagtcatcc attgccaaaa cctcggtcat
      721 ttgaaccgat aggtgatgcg gcccttggtg aggtcgtaag gcgtcatctc aacaaggact
      781 ttatcgccgg taagaacgcg gatgcggttc ttgcgcattt ttccggcggt gtgggcgatg
      841 atctcatgct cgttttcgag cttgacccgg aaggtcgcat tcggaagcaa ttcgataacg
      901 gttccaggaa attcaagcaa ttcttccttc gacatttgtg tccttagctt taggccgccg
      961 ttggtctgcg ccagcctccc aggctacccg cgcggaccct agacgagaac tccgcttttg
     1021 tgaactgctc aacggtctgt cagcttacgc cctaccctgt cgctggcgcg cacagctttc
     1081 catcgaggac gcctttgcag tcgagaggag aagaccttgc aacgatgtcc gtcattttcg
     1141 aacttgcaag gcaggccctg gcacgggagt ggccgaagcc tgacgggcgg cgtccgccgc
     1201 cacataggct ttgaagcggc gcatcgcgaa gggaatcgtt ccgagataga ttaaggaaag
     1261 gccaatcagc atttccattg taaaggttgc cagcaagagc aagcaggcga cgattccaaa
     1321 aaggaatgca aggacatatt cacgtggcac acgaccaacg ttcttgcccg aaaaatgcgg
     1381 gattgggctc gccatcaaaa gagcaatgag taagacatag gcgatttcga acggtgccac
     1441 ggaacgtgtg tttggaacag caagaataga aagattaaga taaactggta agagaccgac
     1501 aatcgcgcct gctggcgcgg gcatcccggt gaaaaaatgc gcgtgccacg ctggccggtt
     1561 cggatcgtcg atcatgacgt tgaagcgggc gaggcgcaag gcccccgcga tggcaaaaac
     1621 gagggccgcg taccagccaa aactcttaat ttcatgcaag ccccagaaat aaagtatgag
     1681 cgcaggcgcc actccaaaat cgacaaagtc ggcgagcgaa tcgagttctg cgccgaaccg
     1741 agacgtgcca cgcagcgccc gcgcgagacg gccatctaag ccatcaagta tggcggctcc
     1801 gataacggca accaccgctg tttcatattg gccgtcaacc gcgaatcgga tcgcggttag
     1861 ccccatgctc agcgccagca aagtgacgag attgggcaag ataatgcgca ttggtaccgc
     1921 gcgaagcccg cgtttgcggc gctgcatcgc caagccctcg aacgggctgc gatcgccgta
     1981 aagcgaggtc agctctgcgc catgatctgc gctcggactt gaattgtctt tcgagtttat
     2041 attcatgagc gcagcataag gaggcagaat ccccgccgca aggtttgttt cccggcagcg
     2101 ctgccagaca caggctcgcc gcagagcgcc cgcgacaatt ccgctgcgct ctatgataac
     2161 gtggctgcgc tcgtgagaga tttcttggaa tgatttttgt agcccagcag aatagcattt
     2221 aagagcttcc taagcgatta tgagcttgac ctttaccgga attgcataat ctcgtgcagt
     2281 ttaggctgtt ggcagcgtgt caagatagac aggagacgtt cgtggcggat gtagaggaaa
     2341 ttgaacaagc tctttcctgg gcaataaaaa agcgcatgct ggaggggtgc ggaacgctga
     2401 gcaaggtcag catgatcgat ggaattgtgc aggttacctt caatggcgtg acggacgata
     2461 cgcatgtatt tgatactcta agcggggcgt tgcttgattt gcgccttggg ttggatattg
     2521 tgcccgacct cgacgtgaaa attcggcgca agtggcgctt aatgccgcag cgttgaggcg
     2581 gctcgccatc cttaggccga ggaacaatgg aaccggcagc ctcctcggcc caaggatcct
     2641 ttgtcggcgt ccgtctgcac attggcaaac cgttcccctt ggcccagatc tcgactagag
     2701 atgcttgacc cgggaaggct cgggaacagc ggcagctcta aggatgcact ccggctgatg
     2761 gccggccggg gggtgctgta tgtgtgattt gacgagacca catgcccaaa cgaacggata
     2821 tttcgacgat cctaatcatc ggagccggcc cgatcatcat cggccaagct tgcgaatttg
     2881 attactctgg cacccaggcc tgcaaggcac tccgtgaaga gggctaccgg attgttctcg
     2941 tgaattctaa tccggcaacg atcatgaccg acccggacat ggccgaccgc acctatcttg
     3001 agccaatcac gccggaaatc gtcgccaaga ttatcgccaa ggagcggtac gtcgttcctg
     3061 gaggatttgc gcttttgccg acaatgggcg gccaaactgc actgaattgc gcgctctcct
     3121 tgaggaaaat gggggtactc gaaaaattct gggtggaaat gatcggcgcc agcgccgcgg
     3181 caatcgacat ggccgaagat cgcaagctct tccgcgaggc gatgacccgc cttggtcttg
     3241 cgacgccgcg ctcgcatcaa attaagacct tgccgcaggc acttgcggtc ctcgacgata
     3301 taggcctgcc cgcgatcata aggccctctt tcaccatggg gggaaccggg ggcggaattg
     3361 cctataacaa ggctgaatat atcgacattg cggagcgggg catcgacgcc tcgccgacga
     3421 atgagatttt ggtcgaagaa agcgttctcg gctggaaaga atttgagatg gaggttgtcc
     3481 gtgacaaggc ggacaattgc atcattgttt gctcgatcga aaacatcgac ccgatgggtg
     3541 tgcacacggg cgattcgatc accgtagctc cggcgctgac tctcaccgac aaggaatatc
     3601 agaccatgcg tgacgctgcg attgcggtgc tgcgcgagat tggtgtcgag acgggcgggt
     3661 ctaatgttca atttgcggtt aatccaaaag atggccgcat ggttgttatt gaaatgaacc
     3721 cccgcgtctc gcgttcctca gcgctggcgt ccaaggcaac gggattcccg attgccaagg
     3781 tcgccgcaaa actcgcgatt ggtttcacgc tcgatgaaat cgccaacgat ataaccggcg
     3841 gcgctacccc agcctctttc gagccttcga tcgactatgt tgttaccaag attcctcgtt
     3901 ttgcttttga aaaatttccc ggcgcagagc aaatcctgac gaccgcgatg aaatcggtcg
     3961 gcgagaccat ggcgatcggc cgtactttcg ccgaaagcct gcaaaagggg ttgcgctccc
     4021 tcgacacagg actggatggt ctaaacgaga tagagctcga gggcctgggg cttggcgacg
     4081 acatgaatgt gctgcgcgcc gccttgggta taccgacccc agaccggttg cttgttgttg
     4141 cccaagcgct tcgccatggc atgccgcaag atgagattgt tgaggcttgc ggcatcgatc
     4201 cttggttcat cgaacgtatc gcggaaatcc ttgatctcga agctagggtt cgcgcacatg
     4261 ggttgccggc cgacgcgacc aatctcagga agttaaaggc ggcgggattt tctgatgcgc
     4321 gtcttgcaaa tcttgctggt gcgccggaag caaaggtttc ggctttgcgc aagaaactcg
     4381 gcgtgcggcc ggtgtataag cggatcgata cctgcgcggc cgaattttct tcgcccaccg
     4441 cctatatgta ttcgacctac gccccgcctt ttggttcggt cccggcctgc gaatcgcgcc
     4501 cctcaacgcg caagaaaatc gtcgtccttg gtggcgggcc gaaccggatc gggcaaggga
     4561 tcgagttcga ttattgctgt tgccatgcga gctttgcgct gcgcgaggca ggttacgaaa
     4621 cgatcatgat caattgcaat cccgagacgg tatccaccga ttacgatacc tccgatcggc
     4681 tctatttcga accattgacc gccgaggatg ttcttgcaat tcttgccaac gagggtgaga
     4741 caggcgagct taagggtttg attgtgcaat ttggcggaca gacgccgctc aaacttgcgc
     4801 atccgctcga acaggctggc attccaattc tcggaacatc ggttgattcg attgatcttg
     4861 ccgaggatcg cgaccgtttc aaacggctct tggataaggt cggcttgaag cagccgatga
     4921 atggcatcgc ctattcggtg gaacaatctc gcctcgtcgc gtcagacctt ggacttcctc
     4981 ttgtcgtgcg tccctcctat gtactcggcg ggcgggcgat ggcgatcatt ttcgacaacg
     5041 ccgcattcga tgattatttg cttggcacgc ttccgggcct cgtcccctca gaggtgaagg
     5101 cgcgctatcc taatgacaaa accgggcaga ttaataccgt gctcggcaag aacccgctct
     5161 tgttcgaccg ctatctgtca gacgccaccg agatcgatgt cgacgcactt tcggatggca
     5221 aggatgtatt catttgcggg gtcatggaac acatcgaaga ggcaggcatt cattccggtg
     5281 atagcgcttg ttcgctgcct ccgcgctccc tctcgcggca aacaatttca gaactcgaat
     5341 atagcacaaa aaagctcgcc ctggcgctcg gtgttggcgg cctgatgaat gtgcaatatg
     5401 cccttaaggg cggagaaatt tacgtgcttg aagtcaatcc gcgcgcctcg cggacggtgc
     5461 cttttgtcgc caaggtcgtc ggtctgccgg tcgccaaaat tgcggcccgg attatggctg
     5521 gggatagttt ggcgagtttt ggcctgcgcc ccagggagtt tgaccacatc ggcgtcaagg
     5581 agtcggtatt tccatttgcc aggtttccgg gggttgatac cgtgctcggg ccggaaatgc
     5641 gctcgactgg tgaggttgtc ggccttgatc ggtcattccc gattgctttc gccaagagcc
     5701 agatcggcgg gggcacgaat gtccccagaa ccggtacggt cttcgtttcg gtgcgtgata
     5761 ttgataaacc tcgtgtcgtg gaaaccgcgc gattgctgac aagcctcggc tttaaggtcc
     5821 gagctacggg tggcaccgcg cgctacctcg aaagcgcggg ggttgaagct ctgcgcgtca
     5881 acaaggtttc agagggccgg ccccatgtcg tcgacttgat caagaatggt gacatccaac
     5941 tcatgcttaa caccaccgaa ggcgcgcagg ccttggccga ttcgcgatcg ttgcgccggg
     6001 cagctctctt gcataaagtg ccctattata cgacgctcgc tggggcgatt gctgcagcgc
     6061 aaggaatccg cgcctatctc gcaggcgatc tcgaagttcg cgctttgcag gattattttg
     6121 cctagagcgt ggctataccg gtgcacttgc ccaaaaacga gccagtgcag ccttcccctt
     6181 gcgatctgct tcgtcagggt gtctgtacag tcttacttgg atagtagagc cgatttcggg
     6241 agttcccgcc ggcagccagc attgcatccg taactcaaca tcacaaagga caatgggaaa
     6301 atggtaccac tcccatggca catagcaagc aaggcgaccg acgggcctta aactttggac
     6361 ataaaggttg gacatgaagg agagcatagt cttcaaacca ggaaccggat tcttaacaag
     6421 atcgtgcaac atcaaaactc cgaggtctcg ccgggacgat acgacggatt cggaacgggc
     6481 acggggagca tcgacaaagt aaaagagaat aagcatggac aagatcccca tgacggcggg
     6541 tggatattct acccttgaag aagagttgaa gaggcgccag cagattgagc gtccacgcat
     6601 catccagacg attgcagaag cgcgggcaca tggggatttg tccgaaaatg cggaatatca
     6661 ctccgccaag gaagctcaag ccttgaatga aatgcggatc gccgagctcg aggacaaatt
     6721 atcgcgcgcc gaagtaatcg atatatcaaa actgtccggc aataccatca agttcggtgc
     6781 aacagtgacg cttgtcgatg aagacacgga cgagaagcgg gtttttcaga tcgtgggcga
     6841 aaacgaggca gatgtgaagg ccggccgggt ctcgatcact tccccgacgg cgcgtgccct
     6901 gattggcaaa aaaaccggcg attcggtgga agtgaatacg cccggcggcg gcaagagcta
     6961 cgaaatcctc gacgtagctt ttcaataaag tgccgcgcga gggcgccgcg tttctgagca
     7021 caatccaaag ccgtgaaagc tgcgcccgca tattgcttgc gggagccacg atgagtctca
     7081 cttcaagaca gacgtgctta atggaccagc cttgcaacaa actttttcac cgcttcgaag
     7141 tcatgggctt ggataagatc aacgcatctt taaaacgggc ataccatcgc cactatggcc
     7201 tgtcgcaaag gcaattggga tacgaagctt ataaaggaca tcggtcaccg gaaaggagtc
     7261 ttcgcgatcg agcgacacgt caagaattgc gccatccacc atgg
//
LOCUS       DATMPU010000258         2537 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_3301998,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000258 DATMPU010000000
VERSION     DATMPU010000258.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 2537)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2537)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2537
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3301998"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            284..622
                     /locus_tag="VNY06_05905"
     CDS             284..622
                     /locus_tag="VNY06_05905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012384644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1491 family protein"
                     /protein_id="HWX14377.1"
                     /translation="MRLRADFWVAAYIRRCAHEGASAVLCRRGAPEAGAIFIKIDSLN
                     GSVALYGPAPQSELGDNHERLFARLHSAPWINPAEADRTIASQIRFDPDLWFVETEDR
                     LGRSFLEVIE"
     gene            complement(729..1736)
                     /locus_tag="VNY06_05910"
     CDS             complement(729..1736)
                     /locus_tag="VNY06_05910"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012384309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HWX14378.1"
                     /translation="MRLDRWFKRRIPTFSLSHLNKIVRTGEVRVDGSRVKTSTRLTAG
                     QSVRVPPLKIIAPVLPARKISPAEICALQDIILFEDRDLLVFNKPFGLAVQGGSGQTR
                     HIDGMLAAMPNERGDRPVLVHRLDRDTSGVLLVAKTRKIAAELGEVFRSRQARKIYWA
                     LVEGVPKPAQGRISLYLAKGDGMEKDGSEWRGTDTRGRLEKMRIASHGDEDARHSVTY
                     YAIVDKAPPRLAWLSLKPTTGRTHQLRAHAEAIGHPIVGDPKYGARPATGLCQSNPLR
                     MVPEAIECKLHLLARRLVLPHPRGGVLDVTAPLPPHMQRTFDIFGFDTKRFDPIQNAP
                     E"
     gene            complement(1868..2107)
                     /locus_tag="VNY06_05915"
                     /pseudo
     CDS             complement(1868..2107)
                     /locus_tag="VNY06_05915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020174674.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
BASE COUNT          559 a          738 c          683 g          557 t
ORIGIN      
        1 cgaaagttca gaatcggcag gcataaaatc gggcacggcg ttagctgcat tggtggcggg
       61 agaaccagtc acgcttgatc ttagcacgca agtgccctaa catttcattt cactttctgg
      121 caaaccgccg gttgccaggg aagacgcgga cactttcatg tcattcgcgc gattgaacgc
      181 cccgacgatt ctccaatcgc gatcactccg gacgagatcg tccacgtttc gccggttccg
      241 gcaagcggcc cgctgatggg actgctcacc aaaggaacca ggaatgcggc tacgtgccga
      301 tttttgggtg gcagcctaca tcagacgatg cgcacatgaa ggcgcctcgg ctgttctgtg
      361 ccgccggggc gctccagaag ctggtgcgat ttttatcaaa atcgacagtc tcaatggttc
      421 tgtagctctt tacggtccag cgccacaaag cgaactgggc gacaaccacg aacggctctt
      481 cgcgaggctt cattctgctc cgtggattaa cccagcagaa gccgatcgaa caatcgcgag
      541 tcaaattcgc ttcgatccag acctttggtt tgtcgaaacc gaggacaggc tgggtcgttc
      601 gtttctggag gtcatcgagt gacatggaat atcgtcccac cttgcttcac gtgaagcatt
      661 ttgccatgat tgacggatag acaaattgac atcgccgaga cgagagggcg atcagaggcg
      721 agcaaatctc actctggcgc gttctggatg ggatcaaaac gcttcgtatc gaagccgaaa
      781 atgtcgaacg tgcgttgcat atgcggcggt agaggcgcgg tgacatcaag cacgccgcca
      841 cgcggatgag gtagcaccag gcggcgcgcc agaagatgca gcttgcactc aatcgcctcg
      901 ggtaccatcc gcaaaggatt gctttggcac aagcccgttg cgggcctcgc gccatatttc
      961 ggatcgccaa caatcggatg gccgatcgcc tcggcatggg cgcgcaattg atgcgtgcgc
     1021 cccgtagtcg gtttaagcga aagccaggct agccggggcg gcgctttatc gacgatcgcg
     1081 taataagtca ccgaatggcg ggcatcctcg tcgccatgcg aggcgatacg catcttttcc
     1141 aacctgccgc gtgtatctgt gccccgccac tccgaaccgt ccttctccat gccatcgcct
     1201 tttgcgagat aaagggaaat tcggccttgc gctggcttgg gcacgccctc gacgagcgcc
     1261 caatagattt ttcttgcctg acgggagcgg aaaacctcac caagctcggc cgcaattttt
     1321 cgcgtcttag caacaagcaa tacgcccgag gtgtcgcgat ccagcctatg caccagcaca
     1381 ggccgatcgc cgcgctcatt tggcattgcg gcgagcatcc catcgatatg acgggtctgt
     1441 cctgagccgc cttgaacggc aagaccaaac ggcttgttga acacaaggag atcgcggtcc
     1501 tcaaagagga taatatcttg cagcgcgcat atttcggctg gcgaaatttt tcgtgcagga
     1561 agaactggtg caatgatctt taacggaggc acgcggacgc tttgtcccgc cgtaagccgc
     1621 gtcgaagtct tcacgcgcga gccgtcaacg cgcacctcac cggtgcgcac gattttattc
     1681 agatgcgaaa gcgaaaatgt tggtatccgc cgcttgaacc agcggtcgag gcgcatgccc
     1741 tcttcatctt cgctaacctc gaatgtttgc acccccaccg agcagccccc agacaagcct
     1801 ttgcttgcgc tcacgaacac gcacttgccc aacaattctc ttccgcggta aattaagctt
     1861 acgtcttcta ttgggctgca gtcgaagata ctactctata agagctgcaa attgacgctt
     1921 tgggtccttt gcccttctta tcaggctcga tctcgttgct cgcccgctgg ccttcatcaa
     1981 gggatggaag tctgcctgct cgaccgcggc actaattcac aaaaatatcg cgtcccccgc
     2041 tgtccgattt gatgaagccg tagccgccct caccattaag gaatttgtct atgccctgct
     2101 gcggcacgga taggtgctcc gagagaactt tgcccagtag ggccttgcgc catcctgcgg
     2161 ccctgcacga atccgctttc aagaaattgg gtcagtcgcg agctttggca agcggcctca
     2221 gtcctcgtca gccaatccat cgaatagcta ggctggccat ttccaagcca gcttgcgagc
     2281 cagggcaata ggttttcgct tgattgtcga gctggaacgg cggattgaca atgaagagcg
     2341 cgcacttcac caacggccct tgcggtattg gcgaatcgaa ttaaaattca aggcaaacgc
     2401 tcttttcatc ggcccgtgcg gacggtaaca tcatgatcga gaatagtcca ttcgcaaagg
     2461 cgcttgagga ggtaaatcgc gccaatccct atccgcttta tgctttgctc cgcgagaccc
     2521 ccatatgtcg cgaagag
//
LOCUS       DATMPU010000259         2215 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_3302259,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000259 DATMPU010000000
VERSION     DATMPU010000259.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 2215)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2215)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2215
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3302259"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..630
                     /locus_tag="VNY06_05920"
     CDS             <1..630
                     /locus_tag="VNY06_05920"
                     /inference="COORDINATES: protein motif:HMM:NF021523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2252 family protein"
                     /protein_id="HWX14379.1"
                     /translation="LSQMFASDMVRPTLTSLRGRAKDDPIELLDAAYWMKGCSSLGRL
                     RYAAILRIGHGKNSSLCLADVKEGVTATAPKEPRVEMPRDNAIRVVTGARALSPNLGQ
                     RMMPAKLLGKSVVVRELMPQDLKIEINGLNRQEAINLARYLAGVVGRAHGRQMKASQQ
                     RAWRSDLAKNHKASLEAPSWLWASVVELLGLHEAAYLEHCRRFALSEAA"
     gene            947..1126
                     /locus_tag="VNY06_05925"
     CDS             947..1126
                     /locus_tag="VNY06_05925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018901740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HWX14380.1"
                     /translation="MDREHVEGAADKAKGAIKDAAGKVTGDKKMQTEGKLDKAKGDAH
                     NLAGDLKDAVRKGKE"
     gene            1661..1981
                     /locus_tag="VNY06_05930"
     CDS             1661..1981
                     /locus_tag="VNY06_05930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018068460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HWX14381.1"
                     /translation="MRRSMIAVLFVASAIPAMAQTNMKPANNDARTPAVNTPNTPPNP
                     TAPVAGANSFTEGQAKSRIESKGFKGVSHLKKDDTGVWRGEAMQNDKTVNVSVDFQGN
                     VVAQ"
     gene            2018..>2215
                     /locus_tag="VNY06_05935"
     CDS             2018..>2215
                     /locus_tag="VNY06_05935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018453227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX14382.1"
                     /translation="MTTTISRLYNSQMEARMAVRDLEAAGLKNGDISIVASNADNWYD
                     AKTKTYHDLAGKDDRAEGAAAG"
BASE COUNT          564 a          581 c          639 g          431 t
ORIGIN      
        1 ttaagccaga tgttcgcaag cgacatggtc aggccgacgc tcacttcact tcggggtcga
       61 gccaaggacg acccgattga gctgctggat gctgcctatt ggatgaaggg atgcagctcg
      121 ctcgggcggc ttcgctatgc cgccatattg cgcatcggac acggcaagaa ctcgagtctc
      181 tgcctggccg acgtgaagga aggggtgacc gctacggccc ccaaggagcc gagggtggaa
      241 atgccacggg ataatgccat ccgagtggta acgggcgcgc gagctctttc tccgaacctc
      301 ggacaacgca tgatgccggc aaagcttctc ggaaaatcag tcgttgttcg ggagctcatg
      361 ccgcaagact tgaagatcga gatcaatgga ctgaaccgcc aggaagcgat caatctggcg
      421 cgctatctag ccggagtggt cggacgcgca catggtcgcc aaatgaaagc cagccaacag
      481 cgggcatggc gatccgattt ggcgaaaaat cataaggctt ctcttgaagc cccgtcctgg
      541 ttgtgggcga gcgtggtcga gctgcttggt ttgcacgagg ctgcatatct ggaacactgc
      601 cgacgatttg cattgtcaga ggccgcttga agaagacgcc ggaagaaacg cgggcgaaag
      661 ctaaggagtg cgagcgcgag gcgcttatca aagctaaaac taacgttcca cacgcgtgcc
      721 ggcctgctcc actgcgcccg ggagcgacgc ccaatcgatc gagcaagcta aaaaacttag
      781 caatatcgtg attcttggtt ctgctgttcg gcggcggcgg ctattgggga cgcagacgag
      841 gtttttggta gcgcacattg ttgcgcttgc ggcatttttg cgcttaatgg aacggccgtt
      901 cgcgctggca cgttgtcggt acaccaatcc cacacaggag tcgtacatgg accgcgaaca
      961 cgttgaggga gccgcggaca aggccaaggg cgcgattaag gacgccgctg gcaaggtcac
     1021 gggcgacaag aagatgcaaa ccgaaggcaa gctcgacaag gccaagggtg atgcgcacaa
     1081 tctggcaggc gatttgaagg atgcagttcg caagggtaaa gaataacaaa acaggcgata
     1141 gtgcttcaaa aaagcctgcc ctttgggacg ggctttttct agcttgaatt gcagggtatt
     1201 ccacgaccgt aaccattatg cgtaactttg acggccaact tctcacagtg acgaaagccc
     1261 ggcgaatagt taagcgacac agttcgccta gtttcaagat tcggccgcgg cgcacttatt
     1321 ttgcacctgt cacagccgga atatgaaaca ttccttggcg tgttcgcctc acgcgcccgg
     1381 cgccgccggt atgcgaagat gcaacgaatg taatggaaga ttcgggcttg ttcgacacag
     1441 gttcgccctc aagcaatttt gctcgaaacg ctgcctgcaa aaatatcaaa ttgataacga
     1501 gcggaaagct tctcgcatca agcaatgggc cgactttcta tgcggagcgc gtagatgatt
     1561 aacttcattg ccaggagctt acgaggcggg cagcggttac cttgggaaca tctcacccct
     1621 tggggactta gtctgttacc aatccaaaaa ggaaaaccct atgagacggt caatgatcgc
     1681 tgtcttgttt gttgcttcag ccattccagc aatggcgcag accaacatga agccggcaaa
     1741 caacgatgcc aggacacctg cagtcaacac gccaaacacg ccgcctaatc ccaccgcacc
     1801 ggttgctggc gcaaatagct ttaccgaagg ccaagccaag tcgcggatcg agagcaaggg
     1861 cttcaagggt gtatcccatc tgaagaaaga tgataccggc gtatggcgcg gggaggccat
     1921 gcagaacgac aagacggtga acgtaagcgt cgactttcag gggaacgttg tcgcgcaata
     1981 aagctgctcc catactctaa atcaaaaagg ttcaaccatg acgacgacaa tttctcgcct
     2041 ctacaacagc caaatggaag ccagaatggc tgtgcgtgat ctcgaagctg ctggccttaa
     2101 aaacggtgac atcagcatcg tggcaagcaa cgccgacaac tggtacgacg cgaagaccaa
     2161 gacttatcat gatttggctg gcaaagacga tcgagctgag ggagcggctg ccggc
//
LOCUS       DATMPU010000260        11014 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_2356555,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000260 DATMPU010000000
VERSION     DATMPU010000260.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 11014)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11014)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11014
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2356555"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(235..815)
                     /locus_tag="VNY06_05940"
                     /pseudo
     CDS             complement(235..815)
                     /locus_tag="VNY06_05940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018266181.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
     gene            complement(978..1481)
                     /locus_tag="VNY06_05945"
     CDS             complement(978..1481)
                     /locus_tag="VNY06_05945"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012385849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HWX14383.1"
                     /translation="MDTVNYRPCVGIMLFNRSGLVFVGRRRKENIPERLRQGYEWQMP
                     QGGIDAGEDPYQAALRELREETNVSSTTFLAESPDWYCYDLPSDGWRKASRGRFRGQR
                     QKWFALRFDGHESEINIDEPAGGQKPEFDAWRWETIGRLAELIIPFKRPVYDKVVETF
                     GHLAEMR"
     gene            complement(1490..2722)
                     /locus_tag="VNY06_05950"
     CDS             complement(1490..2722)
                     /locus_tag="VNY06_05950"
                     /inference="COORDINATES: protein motif:HMM:NF016623.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HWX14384.1"
                     /translation="MRQSRGRAIILAALLGSGGLIATFAPSLLEQIRPPAAAASNDAN
                     ISRLAGTVSQKSVPSADMGRIPLLSDLQEESDARRPLHLPSASEPDGHATTGTEAIIE
                     KQTGVKIVRAGGGGAPEALVIDVAKALSAGGTAAADPRIIEQSRYGPIPRIGVDGARR
                     SKVYSRPAPSSRGEPYSPRIALVVGGMGLAARTTETAISALPGEVTLGFAPYGSDLAR
                     QTALAREAGHEVVLQIPMEPFDFPRDNPGPHVLLASAGKAANIDNLTWLMSRFRGYAG
                     VMNFLGGRFTGDQKALTPVLREIAARGLYYLDDGSSSQTIAVTLAANQGLAAAKADIV
                     LDSAAQPEAIEAALFWLEAIARDRGVAIGVANALPATISAIGRFARALESRGGSLIPL
                     SVAIPNQQGGVADSSPMR"
     gene            complement(3158..4528)
                     /locus_tag="VNY06_05955"
     CDS             complement(3158..4528)
                     /locus_tag="VNY06_05955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019401054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HWX14385.1"
                     /translation="MMRKLFPLMLGAALGAGLVVFATRSPVFSEATARAAASDTYRNL
                     NLFGDVFEKIRSDYVERPDEQKLIESAINGMLTSLDPHSSYMDAKSFRDMQVQTRGEF
                     GGLGIEVTQEDGLIKVVTPIDDTPASHAGIQSGDIITAIDGESVQGLSLNQAVDKMRG
                     APDTSVTLKIVRGANKDPQDIKLTRAVIQIKSVRARQEGDDIGYIRITQFNEQTFDGV
                     RAAIQKFQADIPAAKFKGYVLDLRNNPGGLLDQSIAVVNCFLDRGEIVSTRGRNPDET
                     MRYNARPGDLSKGKQVVVLINGGSASASEIVAGALQDHKRATVIGTRSFGKGSVQTII
                     PLGQSNGAVRLTTARYYTPSGRSIQARGIEPDVTVLQDVPDEFKGKDDTKGEASLKGH
                     LKNGDDEKGGSQAYVPPDPKNDKQLIAAEDILRGVVKASNVTSPPGGDAKVTAAPSKE
                     EAKVPN"
     gene            complement(4525..5907)
                     /locus_tag="VNY06_05960"
     CDS             complement(4525..5907)
                     /locus_tag="VNY06_05960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020177384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HWX14386.1"
                     /translation="MAFLARHFRLASVGAARFATLSLFVGSFAPSCIAAQTAQPGAQG
                     TDKKDAELINRRLELRGMADTLGASQQQRQKIEAEIASIHADRAKLTAALLNATQNVN
                     STERKISETEARLDMLTGSEDAIKRSLAGRRALIVEVLASLQRMGRKPPPALLAAPED
                     MLAAIRTSMLLGSVLPQMRGETEALASDLGDLMRLRRSIAAERETLASGVTNLRAERQ
                     RLAALVEARQAALIAAEKALDTERERAAEIAEQATSLRDLIARMETEIAVAARIAEAA
                     RKSDEMRQRAAEAAPSATGMKSAPAPFKDPARLAPAVSFVDTKGLLPFPVNGIIQRGY
                     GSRDAYGGTEKGLLIATRSEAIVTAPCDGWVSFAGPYRSYGQLLIVNAGQGYYIVLAG
                     MDKINVSVGQFVLAGEPVAVMGDSSAKTTAAIAVGAVQPVLYVEFRRDGVAIDPGPWW
                     VKPELQKVRG"
     gene            complement(6020..6502)
                     /gene="rlmH"
                     /locus_tag="VNY06_05965"
     CDS             complement(6020..6502)
                     /gene="rlmH"
                     /locus_tag="VNY06_05965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012589689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HWX14387.1"
                     /translation="MRLLLVCVGRSKTGPERDLATRYIKRAAAAGSVIGFSKVELREV
                     EESRAGFSPDRKRDEARALRSLLTARATVIAFDEVGERITSRGFAALLGRARDEGVAS
                     TVVVIGGPDGLDPAFLDSASRALSLGPMTWPHQLARVMAAEQIYRATTILSGHPYHRD
                     "
     gene            complement(6518..6847)
                     /gene="rsfS"
                     /locus_tag="VNY06_05970"
     CDS             complement(6518..6847)
                     /gene="rsfS"
                     /locus_tag="VNY06_05970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020177386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HWX14388.1"
                     /translation="MQTVLTCLDDSKAEDVVAIDLHGKTTVADIMIIASGRSNVHVGA
                     IAERVIGVFKGEHQKPPRVEGLRNCDWVLVDGADIVVHIFRPEVRKFYNLEKMWSLDR
                     PGERGLG"
     gene            7221..7709
                     /locus_tag="VNY06_05975"
     CDS             7221..7709
                     /locus_tag="VNY06_05975"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF016107.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HWX14389.1"
                     /translation="MQQGPWGIDPAADHPKVIASPALIYAAVLVSGFVIDWLWPLKFL
                     PEGWSLVVGFFLIFIAINIKTYAVREMVRLKTNLNVRKPTTAIATEWPFSVSRNPIYA
                     GILLLNIGIACFVNALSILLLTPVLALLLQKAVIEPEEAYLEQKFGPAYLRYKAIVRR
                     WI"
     gene            7753..7998
                     /locus_tag="VNY06_05980"
     CDS             7753..7998
                     /locus_tag="VNY06_05980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX14390.1"
                     /translation="MQHDRSDRPDVIALPPVILVATIARGLVLNFFVPASFLPKMIAA
                     PLGFSTAFVAIEPEEAYLEQKFGDKYLRYNAKVRRWI"
     gene            8034..9431
                     /locus_tag="VNY06_05985"
     CDS             8034..9431
                     /locus_tag="VNY06_05985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012385161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase family protein"
                     /protein_id="HWX14391.1"
                     /translation="MPRGILSRRLFLGQTFASIMTLPAEATAPALRDSFYTLEASEGK
                     LKLLPEPAAPTNIWGYGGTVPGPLLRIKKGAEVKIRLVNKLPQPTSLHWHGVRISNPM
                     DGVAGLTQEPVLPGGTFDYRFTPPDSGLCWYHPHMLPFLDEQLERGLYGVMIIDEPEP
                     PLADHDMLVVLNNWRLDDKGQIKKFDMLADAIRADWTRPLVTVNAGAVPIEEVLPPSS
                     RLRLRIVNAANGQVMAIAFSGVTPSVLAIDSQPCEAFEPAKNVIPLGPGARFDVMLDL
                     PSDPGATASLSLLDNNELGRILLAFKTTGEKRSPLAPIASLPQNPLLPAEIKLESSHK
                     FNISIEATASAGADPATPSAQDQPLYWRLNGKIFEGLAPPPLFSVKRGTPVTLGFTNR
                     TSIVQAIRVHGHAMRLLHDLDDGWGPYWRDTVLIPQGKTKHIAFVADNPGKWAIECLK
                     TERQAAATASWFEVN"
     gene            complement(9442..10113)
                     /locus_tag="VNY06_05990"
     CDS             complement(9442..10113)
                     /locus_tag="VNY06_05990"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012385164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HWX14392.1"
                     /translation="MPGSDIAIPASLPNVVAALQKGEKVRILAIGSSSTIGNGASSRE
                     KSYPSQLESILEHALKGVDVVIVNRGVAGEVAETTAERIRNEVALRKPDLVLWQLGTN
                     DALARIPSPEFEETVRSTIRWLKDNQIDVVLVGVQYTTRLVRDANYLAIRDSLQKIAA
                     EEKTLYVRRYDAMRFIAQRRANLQLMAGDNFHLNDLGYHCMAEHVAHALIVSLFAKRA
                     RPAQN"
     gene            complement(10200..>11014)
                     /gene="opgC"
                     /locus_tag="VNY06_05995"
     CDS             complement(10200..>11014)
                     /gene="opgC"
                     /locus_tag="VNY06_05995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020177249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="OpgC domain-containing protein"
                     /protein_id="HWX14393.1"
                     /translation="ALYLDAPFLLDWHNASAIFNDPVEAHLGLILLTHQLGYFNILPL
                     YVVLIFIGPAVALLHRAATVLLLLVSVTVYATALVLGLNLPTWPVEGTWFYDPLAWQL
                     IYVLGFLFAGEDGPGRFVRRHRLALRWLAFPVVILGAVAGITNFSPDPVFVPEPKLVF
                     AFSKTFLSPARLIHCLALAMLFAGAFKTIARCIPRLPGYLCLLGRNSLIVFCALSLLS
                     LSGQIFRYVNGGSIATDTFIVILGILLMGFVAWAAEWRDRMRMELASKSPSL"
BASE COUNT         2444 a         3257 c         2986 g         2327 t
ORIGIN      
        1 cccaccgaca actccatccc cactgtggga tggagtcagt gaccgggcgt agccagcgtc
       61 acgtatcttt gcccaagaag accccatctc gtgccgatat tggaactgct ctctgatctg
      121 agcggcgaga gcgattggtc cctcatccgc gagaaaatac caagatttct acgagcctag
      181 gagacgccaa tcagctccgg atgtacgggc ggcttgaggc ggatagaacg tcgctcaact
      241 cgtggcttta cctgcagcca tcgcttccgg cacttcgacc aaccgccctg tcttcacgtc
      301 gtaatagtag ccataaatcg ggatatgacc gggcaccaga ggatgtgatc ggatgcgttt
      361 acgtcctcta ccacacttgc ctttaagtcc ttgaacgtca gccattttac ataatggcct
      421 tgaacactcc ctggaccatg tccgtgatcg tgccatcctt tttcgtcgat ggttgcagtt
      481 tgcagactct gctcgagaag accgctcatg atcgcgtcat cgaagagctg catgccacaa
      541 tcggaatgat gcacgacaaa ccattcctgc gttcccagca acttgtagga aatcacgagc
      601 gaacggatag catcgtcgct ggcgcgtcca cccgcattgc ggattatgtg tgcgtctcct
      661 tccgaaagtc cagcatattt tgcgggatcg agacgtgcat ccatgcaggt aaggatggca
      721 aatcgccgcg cgggcggcaa tgccaattgg gatttggcac caaaacttgc ggcataggcc
      781 tcattggcct tcaagacttc ctgaagaatt ggcatcgtta tgatcctccc tataacaatt
      841 agctagataa gcattagcct cgcctttcat actcatgttg ccgacgcaga gtgcggtctt
      901 ttacggcatc ccaaaggcca cagggaatcg gacccatgcg gacaaagttt ggactgcagc
      961 agcaagcacc gaatatatta gcgcatttca gcgaggtggc caaatgtctc gacgactttg
     1021 tcatagaccg gacgcttaaa cggaataatc aattcggcga ggcggccaat ggtctcccac
     1081 cgccaagcgt cgaattccgg tttctgacct ccggcgggtt catcgatgtt gatttcgctt
     1141 tcgtggccat caaaacgcag cgcgaaccat ttttgccgct gcccgcggaa ccggcccctt
     1201 gaggctttcc tccaaccgtc acttggcaaa tcgtaacaat accaatcggg cgactctgcc
     1261 agaaatgtcg tggaagatac attggtttcc tcacgcagtt cccgcaacgc agcctgatag
     1321 gggtcttccc ccgcgtcgat ccctccttgc ggcatttgcc attcgtaccc ttggcgcagg
     1381 cgctcgggta tgttttcctt tcggcgtcgg ccgacgaaga cgagcccaga acgattgaaa
     1441 agcatgatcc cgacgcaagg gcggtagtta acggtatcca ttgctgccct cacctcatag
     1501 gcgatgaatc tgccacgccg ccttgctggt ttggtatcgc aacactcagc ggtatcagtg
     1561 aaccgcctcg cgactcaagc gcacgggcga agcgtccgat ggccgaaatt gtagccggga
     1621 gcgcattcgc aaccccgatc gccacgcccc tgtcgcgagc gattgcttct agccaaaaga
     1681 gggccgcctc aattgcctct ggctgcgccg cagagtcgag gacaatatcg gccttcgcgg
     1741 ctgcaagacc ttggttcgcc gcaagcgtca cagcgatggt ctgcgatgag gatccgtcat
     1801 cgaggtaata taagccgcgc gcggcaattt cccgcaagac cggagtcagg gccttttggt
     1861 cccccgtaaa tctgccgcct aggaaattca tgacacccgc ataacctcta aaccggctca
     1921 tcagccaagt cagattgtcg atattcgcgg cttttccagc gctggctaga agcacgtgtg
     1981 gtccgggatt gtcgcgggga aaatcaaagg gttccattgg aatttgcagg acaacttcat
     2041 gacctgcctc acgcgcgaga gccgtttgcc gggcaagatc ggatccataa ggcgcaaagc
     2101 ccaacgtgac ttcacctggc aatgctgaga tcgcggtttc cgtggtgcgt gcggcaagtc
     2161 ccatcccacc tacaaccaac gcaatgcgtg gcgaatacgg ctctccccgt gaggaaggtg
     2221 cggggcggga ataaaccttg gaacgtcgag cgccatcaac tccgatacgt gggataggac
     2281 cgtagcgcga ctgttcgata atccggggat cggctgccgc ggtaccgcca gcgctaagtg
     2341 ctttcgcgac gtcgatgacg agtgcctcgg gagcgcctcc accgccggca cgaacgatct
     2401 ttaccccggt ctgcttctcg atgatggcct ctgtccccgt tgtcgcatga ccgtcgggct
     2461 cgctggcgga tggcaaatga agaggccgtc gtgcatcgct ttcctcctgc aagtcggaaa
     2521 gcagaggtat tctacccatg tcagcgctgg gcaccgactt ttgcgatacg gtgccggcca
     2581 atcgcgaaat attggcatca ttggacgctg ctgcagcagg cggcctgatc tgctccagca
     2641 aggacggagc aaaggtggcg attaaaccac cactgccaag gagagccgcc aagatgattg
     2701 ccctgccacg cgattgtcgc actcgaaccc gatcctggcc caatggagcg ttcaagtcgt
     2761 cataccacat ggcggctacc tgaccttggc gcagaaaaaa aactgcgcgg cagtcgcatt
     2821 gccacccgcc aaatggagcg gcgtgatgcc gccacaccct ggctgcttcg ggggaacaga
     2881 cttcgatgtt actttcgtag catctcgctc ccaccaagca agaggtgcaa tggccacgtc
     2941 actgaaacat gatgctatga cgcaaactcc agtcgcagtc ctcctgttca tagcttcgcc
     3001 tgacaagagg gaggccaact tcttgggaac ggaggcaagg gttagtcccc gtgatttttc
     3061 gagtgcgact tccgttcgca tcttcccgaa tcaggcttgc gtggaaccgt ccctccgctt
     3121 acagcgctga ttgaaattga aggccatatc cggaaattta attgggaacc tttgcttcct
     3181 ctttgctagg tgccgcggta accttcgcgt cgccgccggg tggtgacgtg acattgcttg
     3241 ctttgacaac cccacgaaga atgtcttcgg ctgcgatcaa ttgcttatcg tttttaggat
     3301 ccggtgggac ataagcctgt gaccccccct tctcatcgtc gccatttttc agatggccct
     3361 tcagcgaagc ttctccctta gtatcatcct tccccttgaa ctcgtccggg acgtcttgca
     3421 gcaccgtcac atcgggttca attccccttg cctggatcga gcgaccggac ggtgtgtaat
     3481 atcgcgccgt ggtaagcctc accgcgccgt tactctggcc gagcgggatg attgtctgca
     3541 cggatccttt tccgaaggag cgggtgccga tgacagtcgc gcgcttatga tcctgcagtg
     3601 cgccggcgac aatttccgaa gccgatgccg agccgccatt gatcaaaacg accacctgct
     3661 ttccttttga taggtctccc gggcgcgcgt tgtagcgcat cgtctcgtcg gggttacggc
     3721 ctcgcgttga gacgatttca ccgcgatcga ggaaacaatt gaccactgcg attgactgat
     3781 caagcaaacc gccaggattg ttgcgcaagt caagaacata tcccttaaac ttcgctgctg
     3841 ggatatcggc ttgaaatttc tgaattgccg cgcgaacccc gtcaaaagtc tgctcgttaa
     3901 actgcgtaat tctaatgtag ccgatatcgt cgccttcctg acgcgcgcga acggatttga
     3961 tctggatcac cgcccgggtg agcttgatat cttggggatc cttgttcgcg ccacgaacaa
     4021 tcttgagcgt taccgaggtg tctggcgccc cgcgcatctt atcgacagcc tggttgagac
     4081 ttaggccctg gacactctct ccatcaatag cagtgatgat gtcgccggat tggattccgg
     4141 cgtgcgaggc tggcgtgtcg tcgattggcg taacaacctt gataagcccg tcttcctggg
     4201 tgacttcgat gccaagccca ccaaactctc cacgtgtttg gacctgcatg tcgcggaagc
     4261 tcttcgcatc catataactc gagtgaggat cgagcgaagt gagcatgcca ttaatcgccg
     4321 actcgatgag cttttgctca tccggccgct cgacgtaatc cgagcgaatt ttctcgaata
     4381 catcgccaaa gaggttgaga ttgcggtagg tgtcggaggc ggccgcacgc gccgttgctt
     4441 ccgaaaaaac gggtgacctt gtagcgaaaa caacaagccc tgcgccgaga gcagcaccga
     4501 gcattagagg aaacagtttg cgcatcatcc gcgaaccttc tgcaattccg gctttaccca
     4561 ccatgggccc gggtcaatcg caactccgtc cctacgaaat tccacataaa gaacgggctg
     4621 gaccgcgcct accgcgatgg cagcagtcgt ctttgcggaa ctatcgccca tcacggcgac
     4681 gggctcacca gcaagcacga attgcccgac acttacgttt atcttatcca ttccagcaag
     4741 aacaatatag tagccctggc cagcattgac gattaaaagt tgtccatagc tgcgatatgg
     4801 gccggcaaat gacacccaac catcacaggg tgccgtgact atggcttcac ttcgggtcgc
     4861 gatcagcaag cctttctcag tgccgccata cgcgtcgcgg cttccgtagc cgcgttgaat
     4921 aatgccgttc accggaaacg gcaggagacc ttttgtgtcg acaaaggaga ctgccggagc
     4981 taatctcgcc gggtccttga acggcgccgg tgcgcttttc atgccagttg ctgatggtgc
     5041 cgcttccgcc gcccgctgcc gcatttcgtc ggacttacgc gcggcttctg caatgcgcgc
     5101 ggccaccgca atctcagttt ccatcctcgc aatgagatcc ctgaggctag ttgcctgctc
     5161 ggcgatctcg gcagctcgtt cacgctcggt atcgagtgcc ttttcggcag caataagagc
     5221 tgcctgccgc gcttcgacga gagcggctaa gcgctgacgc tccgcacgaa ggtttgtaac
     5281 gccacttgca agtgtctccc gttcggctgc gatcgaccgc cgcaaccgca tcagatcccc
     5341 taggtctgat gcaagcgctt cggtttcacc ccgcatctgc ggcagaacag aacctagcag
     5401 catcgaggtt ctgatggcgg ctagcatgtc ttccggtgcg gccaacagcg ccggcggcgg
     5461 cttgcgcccc atgcgctgta gggacgcgag aacctcaact atcaaggccc ggcggccagc
     5521 aagcgaacgt ttaatcgcat cctccgaccc ggtcagcatg tcaagccgtg cctcggtctc
     5581 gctgatttta cgttcggtag agttgacatt ttgcgtagca ttcagaagcg cggcagtgag
     5641 ctttgcacgg tcagcatgaa tcgacgcgat ctcggcttcg attttctggc gctgctgttg
     5701 ggacgctccg agggtgtccg ccatgccgcg cagttcgagt cggcgattga tcagttccgc
     5761 atcttttttg tctgtgccct gtgccccagg ctgagcggtt tgtgccgcaa tgcagctagg
     5821 cgcaaaggat ccaacgaaga gcgagagcgt ggcgaaacgt gccgccccaa ccgacgcaag
     5881 cctaaaatgc cgtgccaaaa acgccacgag gatccttttg ccgctcacgt gtaaggacag
     5941 gctgcagtca ttttttacat acccgtcatg caacagaaga aagtggcgct ctcgaggcaa
     6001 aattggcaac attgagaaac taatcgcgat gataaggatg gccggaaaga attgtggttg
     6061 ccctgtaaat ctgctcggca gccatgaccc gcgccaattg gtgcggccaa gtcatcggtc
     6121 ccaaagaaag cgcgcgtgac gcggagtcta agaaagcagg atccaagccg tccggtcctc
     6181 cgataacaac aactgtacta gcaacacctt cgtcacgtgc acgacctaat agcgcagcaa
     6241 agcctcggct tgtgatccgc tcaccgactt cgtcaaaggc aatcacagtg gctcgggccg
     6301 tcaacaggct gcgcagcgct ctggcctcat cccgcttgcg atcaggggaa aaccccgctc
     6361 gactttcctc gacttcgcgg agttcgacct tagagaagcc gatcacagag ccagccgccg
     6421 ctgcccgctt gatatagcgc gtggccaaat cccgctcggg tccagtcttg gatcgcccga
     6481 cgcagacgag cagaaggcgc atgccaaaga tgacccctca gcccaagccg cgttcgcccg
     6541 gccggtcgag gctccacatt ttctctaaat tgtagaactt ccttacctcg ggccgaaaga
     6601 tgtgtacgac aatatcggca ccatccacca gaacccaatc gcaattccgc aagccctcga
     6661 cgcggggagg tttctggtgt tcgcccttga acacgccgat aacgcgctcg gcgatcgccc
     6721 cgacgtgaac gttagagcgt ccactcgcga tgatcataat gtcggcgacg gtggttttgc
     6781 catgcaggtc tatagcgacg acgtcctcgg ccttagaatc gtcgagacat gttaagaccg
     6841 tctgcacaag tggtccggct tcaggctcag ctggcccagg aaccggttgg agcggccgga
     6901 gcgccgcttc cgcaggagtc gcgggtaaca gagtgctctt cctccaaata ccccgccagt
     6961 cgggccgggc atgcggctat aaatggcgca gaatcgcgca aatttcaatt ggggcgtagc
     7021 ggctccggat catacccggc gggtcgaccc ataacgcccc gcaaaagtag aactcttgcg
     7081 cgcttacctc gaattggttt gatacgatcg gccagcccct agacgcaaga gccaccgcct
     7141 tccagcgtct tcttatcagt gttagagttc cgtttctggt actgcgaggc ggaagcgcga
     7201 ccgttcgagg aacgtatgca atgcaacaag gaccgtgggg gattgacccg gccgccgacc
     7261 atccaaaagt gattgcctcc cccgcgctga tctacgctgc ggtcttggtg tcgggattcg
     7321 ttatcgattg gctatggccc ttaaaattcc tgccggaggg ttggagccta gtggttggct
     7381 tctttctgat attcatcgcg atcaatatca aaacctatgc cgtgcgggaa atggttagac
     7441 taaaaaccaa cctcaacgtc cgcaagccca cgaccgctat tgcaaccgaa tggcccttta
     7501 gcgtcagccg caatccgatt tacgctggca tccttttgct caacattggc atcgcctgtt
     7561 tcgtcaatgc cttgtcgatc ttgcttctaa cccccgtgct cgcgttgctc ttgcaaaaag
     7621 ccgtaatcga accggaagaa gcctatctcg aacaaaaatt tggtcctgcc tatttgcgct
     7681 acaaagcaat cgtccggcgc tggatctgag gcgagaccat gtaacgggca ggcgccgaac
     7741 tgggagaatg cgatgcaaca tgaccggtcc gacagacccg acgtgatagc cctgccaccc
     7801 gtgatcctag tggcaacgat cgcgcgggga ctggtgctga atttcttcgt gcccgcgagt
     7861 tttttgccaa aaatgattgc cgcgccgctc ggattttcca ccgccttcgt cgccatcgaa
     7921 ccggaagaag cctatcttga acagaaattt ggcgacaaat atttgcgcta caacgccaag
     7981 gtccggcgct ggatctagtc gccgtcattc gatttcaaca cgaaaggaat ttgatgccgc
     8041 gcggaatttt gagccgccgt ttgtttctcg ggcaaacttt tgccagcatt atgacgcttc
     8101 ccgcggaagc gacagcaccc gccctgcgtg acagcttcta tacccttgaa gcgtccgaag
     8161 ggaagttaaa gctcctgccc gagccagccg caccgaccaa catttggggc tatggtggca
     8221 cggtgcctgg gccgctgctg agaatcaaaa agggcgccga ggtcaaaatc aggctggtca
     8281 acaaactccc gcagccaacg agcctgcact ggcacggtgt cagaatctcc aatccgatgg
     8341 acggcgtcgc cggcctcacg caagagccgg tcctgcctgg tggcaccttc gactaccgtt
     8401 tcacacctcc tgacagcggg ctttgttggt atcacccgca tatgttgcct tttcttgacg
     8461 agcagctcga gcgcgggctc tacggagtca tgatcatcga cgagccggag ccaccgctag
     8521 ccgatcacga catgctcgtc gttcttaata attggagact cgacgacaaa ggccaaatca
     8581 aaaaattcga tatgttggct gatgccattc gagcggactg gaccaggcct ctcgtcactg
     8641 ttaacgccgg cgccgtgccg attgaggaag tgctgccgcc atcgtcccgg ctgcggctgc
     8701 gaatcgtcaa cgccgcaaat gggcaggtaa tggcgatcgc cttttctggg gtcacgcctt
     8761 ccgtcctcgc gatcgatagt cagccgtgcg aagcttttga gcctgccaag aatgtgatac
     8821 cgctcggacc cggcgcgcgt ttcgatgtca tgctcgatct tccaagcgac cctggagcca
     8881 cggcaagttt gagcttgctt gacaacaatg agctcggccg catacttctc gccttcaaaa
     8941 ccaccggaga aaagcgctcg cctttggccc cgatcgcttc gctgccgcaa aaccctctcc
     9001 tgcctgccga aatcaagctg gagtcctcgc acaaattcaa tatttcgatc gaggcgactg
     9061 cgagcgctgg ggccgaccct gcgaccccat cggcgcaaga ccagccatta tattggaggc
     9121 taaacggaaa gatttttgag ggactcgcgc cgccgcctct cttctcggtc aagcgcggga
     9181 cgccggtaac actcggcttc acgaaccgca cgagtatcgt ccaagcaata cgtgtgcatg
     9241 ggcacgcgat gcgcctcctc cacgatctgg acgatggctg ggggccctac tggcgcgaca
     9301 ccgtcctcat tccgcaaggc aagaccaaac atattgcctt tgtcgcagac aatccaggca
     9361 aatgggccat cgaatgcctc aagacagagc gccaggccgc agccacggca agctggttcg
     9421 aggtgaacta atcgagatcg atcaattctg tgcgggccgc gctctcttgg caaaaagact
     9481 gacaatcaac gcatgcgcaa catgctccgc catgcagtgg taaccgagat cgttgagatg
     9541 gaaattgtct cccgccatca attgcaaatt ggctcgtcgc tgggcaatga accgcatcgc
     9601 atcatagcgg cggacataaa gagttttctc ctccgcggcg attttctgca aactatcgcg
     9661 gatcgcgagg taattcgcat cacggacaag acgcgtggta tactgcacgc cgacgagcac
     9721 cacgtcgatt tggttatctt ttagccaccg gatcgtcgac cgcacagttt cttcaaactc
     9781 aggcgatgga atgcgagcga gtgcgtcatt tgttcccaat tgccagagaa cgagatcggg
     9841 ctttctcaga gcgacctcgt ttctaatccg ctccgccgtc gtttccgcaa cttcgccggc
     9901 gacgccgcga ttgacgatta ctacgtcgac gcccttgagt gcatgttcca ggatagattc
     9961 gagttgtgat ggatagctct tttccctgga cgacgctcca ttcccaatcg tcgaggagga
    10021 gccgatcgcg agaattcgca ccttctcccc tttctgcagg gccgcaacca cattgggaag
    10081 ggaggccgga atggcaatgt ccgatcctgg cacatcgcag ggtggactca taggtggcgg
    10141 ggctggagga gagacaacct cctccgcatg gagacaagcc attgagagcc agcagatcgc
    10201 tagagcgagg gcgacttgct tgccaattcc attcgcatcc tgtcgcgcca ttccgctgcc
    10261 catgcgacga accccataag gagaatcccc aaaataacga tgaacgtatc agttgcaata
    10321 gacccgccat tgacgtagcg aaagatttgg ccactcaggc tgagaagaga caaggcacag
    10381 aacacgatca gcgaattgcg gcccagaagg cacagatagc ccggaaggcg cggtatgcac
    10441 ctggcaatcg tcttgaaagc gccagcgaaa agcatcgcca gggcaagaca atggatcagc
    10501 cgcgccggcg ataaaaaggt tttggagaag gcgaatacaa gtttcggctc agggacaaag
    10561 accggatctg gggagaaatt ggttatcccc gccacggcgc cgagaatcac caccggaaaa
    10621 gcgagccagc gcaaggccaa ccggtgacgc cttacgaatc ttcccgggcc atcctcgccc
    10681 gcgaacaaaa aaccaaggac atagatgagc tgccatgcga gcggatcata aaaccaagtt
    10741 ccctcaaccg gccaggtcgg caaatttaaa cctagaacga gggcagttgc gtagactgta
    10801 accgagacca gcagcaatag gaccgttgca gcgcggtgaa gcagggcgac cgctggacca
    10861 atgaaaatta agacgacata aagcggaagg atgttgaaat agccgagctg gtgtgtaagc
    10921 agaatgagac ctagatgcgc ctcaactggg tcattgaaga tggctgaagc gttgtgccaa
    10981 tcgagtagga acggcgcatc aaggtatagc gccg
//
LOCUS       DATMPU010000261         6524 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_3621353,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000261 DATMPU010000000
VERSION     DATMPU010000261.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 6524)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6524)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6524
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3621353"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..926)
                     /locus_tag="VNY06_06000"
     CDS             complement(<1..926)
                     /locus_tag="VNY06_06000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018405899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HWX14394.1"
                     /translation="MTSVWALASLWLGLALVASLFSNWFRVSTALSEIIVGTVAQLII
                     GAVIGSAVVGINKSWVKFLSGVGAILLTFLAGAELDPQVFKLKWKEAITVGLASFIFP
                     FLGCAAAAHYLLGWQVMPSWLAGVAMSTTSVAVVYAVMLEFGFNTTEYGKTILAACFV
                     TDLGTVVALGLIFAPITVRTLIFLGAGAIVFVVLPWLTPRFFKRFGGRPYELETKFLL
                     FCLLGMGALAGWADSEAVLPAYLIGMALAGSVGKDHALIRRLRTLTFGLLTPFYFIRA
                     GSFVSIPALVAAPAAFIFFLIIKIVTKVVGVYP"
     regulatory      complement(1084..1144)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01734"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Fluoride riboswitches increase expression of
                     downstream genes that help mitigate the toxic effects of
                     very high levels of fluoride; They are found in a wide
                     variety of bacteria and archaea; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="fluoride"
                     /db_xref="RFAM:RF01734"
     gene            complement(1163..1327)
                     /locus_tag="VNY06_06005"
     CDS             complement(1163..1327)
                     /locus_tag="VNY06_06005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX14395.1"
                     /translation="MIRSCAALANGKVATKLYLLVMLGSALAAPIVTGFEWGGGSMQL
                     PLAFFNLAIA"
     gene            complement(1418..1588)
                     /locus_tag="VNY06_06010"
     CDS             complement(1418..1588)
                     /locus_tag="VNY06_06010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX14396.1"
                     /translation="MLQKKPPLDPKERDKRAAKVLWYILMALRFIIPAYMLYVVYLYW
                     TVPFAPYLQGPR"
     gene            complement(1691..1819)
                     /locus_tag="VNY06_06015"
     CDS             complement(1691..1819)
                     /locus_tag="VNY06_06015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX14397.1"
                     /translation="MRAGHVTEHQLIELMGVTLSKLGTESETELSEIVMAYDIQFY"
     regulatory      complement(1931..1991)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01734"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Fluoride riboswitches increase expression of
                     downstream genes that help mitigate the toxic effects of
                     very high levels of fluoride; They are found in a wide
                     variety of bacteria and archaea; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="fluoride"
                     /db_xref="RFAM:RF01734"
     gene            2095..2457
                     /locus_tag="VNY06_06020"
     CDS             2095..2457
                     /locus_tag="VNY06_06020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012490098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HWX14398.1"
                     /translation="MTDIRIKRIYEPPAPSDGKRILVDRLWPRGMAREEAHVDAWIKE
                     VAPSNALRRWFGHDSEKWGEFKLRYVAELRHNPAVAELREAVPASQSITLLFAAKDCL
                     HNNAIVLRDFLCAGKAEP"
     gene            complement(2461..3498)
                     /locus_tag="VNY06_06025"
     CDS             complement(2461..3498)
                     /locus_tag="VNY06_06025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012383127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HWX14399.1"
                     /translation="MDQNVLGCVAEAEAGGGLCGEIDADRSAAYVAAEEGDHRLLDAY
                     SEAVTGAVGRVGPCVVRLAIASGGRGRSGSGSGVILSPDGFVLTNSHVVQGAKRAEVT
                     ALDGRIFSGRVLGDDPDTDLALVRIDENAILPAARLGNSKKLKPGEIAIAIGNPFGFD
                     ASVTAGVISALGRSLRSKNGRLIDDVIQTDAALNPGNSGGPLVSAHGDVIGINTAIIS
                     PAQGICFAVASNTASFVLGELIAHGRVRRAYLGIGAGTVPLPRRIALRLGLTQAMGAV
                     VSAVEAGSPADHAGLLTGDILLAVDGKEITGADDLVRLLDAEKIDRTVPFDVLRRSDQ
                     RRFWAALRERK"
     gene            complement(3730..4758)
                     /locus_tag="VNY06_06030"
     CDS             complement(3730..4758)
                     /locus_tag="VNY06_06030"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013503399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HWX14400.1"
                     /translation="MRILVVTDAWKPQVNGVVRSLESVGRALREIGTDIDFLTPQGFA
                     TVPLPTYDDIRLALASPWAVAKRLDAAAADHIHIATEGPLGLAARRYCLRTKRVFTTS
                     YHTRFPEYITARTRIPEEITYALLRRFHNAGYGIMVSTKSIADDLKSHGFQRLMRWSR
                     GVDNSQFNPSKAKMLDLPRPIFLYAGRLAPEKNLEAFLSLDLPGSKLIAGDGPSRRAL
                     QASYPQAHFIGMQDPDALAVYYASSDVFVFPSLTDTFGIVLIEALACGLPVAALPVPG
                     PLDVIGTSGAGVLDTDQRAACLAALEIPREEARAHALTFTWANSARQFLDNVNFARSA
                     AEHRPDAA"
     gene            complement(4758..5585)
                     /locus_tag="VNY06_06035"
     CDS             complement(4758..5585)
                     /locus_tag="VNY06_06035"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016921069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HWX14401.1"
                     /translation="MNQEHEPEPTVYRTLFLSDVHLGTRGCQAELFLDFLKYNDAATI
                     YLVGDMIDGWRLKSGWYWPQTHNDVLQKLLRKARKGSRVIFLPGNHDEFARGYIGMVF
                     GGVEVVDHAYHVTADGKRLLIIHGDQFDIVVQHARWVAFLGDWAYTVALWSNLWFNKA
                     RRAFGFPYWSLSQWAKLKVKNAVNFIGAFEATLAEAARRHHADGVVCGHIHHAVIRDI
                     DGITYVNTGDFVESCTAAAEHHDGRLEILHWQRTEEEQRLAADAQQDSARSAAKAAA"
     gene            5805..6422
                     /locus_tag="VNY06_06040"
     CDS             5805..6422
                     /locus_tag="VNY06_06040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018406678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HWX14402.1"
                     /translation="MDVVGRDKAKALEIGAAAVSSQLGKTVQRLRKAYNLTLSELSEQ
                     SGVAKSIISQIERNETNPTLATIWRLSQALDISIERVLKAADDEPFLEKTSRGETPIL
                     VSDDGKCKLSIIGWIKTVEWVQWYGFSAEPGGVLESEAHQRGSVESLSVSAGELEVEV
                     GGATERAKAGETLRYRCDRPHTIRNISQEPASATMVCILKAAAMQ"
BASE COUNT         1541 a         1857 c         1869 g         1257 t
ORIGIN      
        1 ggataaacgc caacgacttt ggtgacgatt ttgatgataa ggaagaaaat gaaggcagcc
       61 ggcgccgcga caagtgccgg aatcgaaaca aaagaacccg cccggatgaa ataaaagggc
      121 gtcagcaatc caaaagtcag cgtacgcaaa cgccggatca atgcgtgatc cttgccaacg
      181 cttccggcaa gcgccattcc aatgagatag gcaggcagaa ccgcttcgct gtcggcccag
      241 ccagcgagcg cccccattcc gagcaagcag aaaagcagaa atttcgtctc aagctcgtaa
      301 ggccgccctc cgaaccgctt gaagaagcgc ggcgtgagcc atggaaggac gacgaagaca
      361 atggcgccag ccccgagaaa gatcaaagtt cttaccgtga ttggcgcgaa gatgagacca
      421 agcgcgacga cggtaccgag atctgtcacg aagcacgccg ccaaaatcgt tttgccatac
      481 tcggttgtat tgaagccaaa ttcaagcatc acggcataga cgacggcgac cgaggtcgtc
      541 gacatggcaa ccccggcaag ccagctcggc atgacctgcc aacctaggag ataatgagcc
      601 gctgctgcgc atccgagaaa tggaaaaatg aagctcgcga gcccaaccgt gatcgcttcc
      661 ttccacttga gcttgaaaac ctgtggatca agttcggcgc ccgccagaaa cgtcaggagg
      721 atcgctccga caccggacag gaacttgacc caggatttat tgatgccgac tacggccgag
      781 ccgatgaccg caccaatgat caattgcgcg accgtcccga caatgatttc ggaaagagcg
      841 gtcgacacgc ggaaccagtt cgaaaacagg ctcgcgacga gcgcgagacc caaccataac
      901 gatgcgaggg cccaaacgct agtcatgagc agtcctcaca cttgcggaca ggatgaaagg
      961 cggggcggcc ggaacttgtg ccgggcttgc cggaagaccc cgaccaacgg gaatcggtat
     1021 gggcgaaaaa gctggaaaac ccgaaattct tggcgggcac ataacaaaac tcctgcctgt
     1081 tgttgcacgg caggagtcat cagcctttac ggcggttttg gggaacccca tccccaggcg
     1141 acgacccgat actaaatgag aatcacgcaa tggcaagatt gaaaaacgcc agcgggagtt
     1201 gcatgctgcc acctccccat tcaaaaccag taacgatcgg cgccgcaagt gcgcttccca
     1261 gcatgaccaa aaggtagagc ttggtggcga cttttccgtt cgccaaggcc gcgcaggaac
     1321 ggatcacggc gttaggtgcg aagtcagctt agatcgcgcg aggaagtcca ggggaacggc
     1381 tttatcgagt cattcaacgc gcccggccgc gcaagcttta acgaggaccc tgcaagtaag
     1441 gggcgaatgg cactgtccaa taaagataga ccacatagag catataggca ggaatgatga
     1501 agcgcagggc catcaaaatg taccaaagca ctttcgccgc gcgcttatcc ctttctttgg
     1561 gatctaaagg tggctttttc tgtagcatcg atgtttcctc atacgttgct gacaactccg
     1621 gatcagagtt ttggttcatc tcatctcgtt taactttacc cttcgcaagg taaccgccag
     1681 ttcggtttta ctagtagaat tggatgtcgt aagccatgac aatctcactt agctcggttt
     1741 ccgattccgt cccgagcttg ctcaatgtga cgcccatcaa ctcgatcagc tggtgttccg
     1801 tcacgtgacc ggccctcatg acctctcacg acggggtcgg caagtaggtc agcgaaagtt
     1861 gccgcattta gatttttgga gaggtacatg ggcaatactc cgccttgttg tgagtgcacc
     1921 cacaaattta ttaaaggcag gagttatcag ccgttaggcg ggcaatgggg aactccatcc
     1981 ccagtcgaaa aatagatctg acggggaact ccgtcaatcc tgcaggcagt ttattgaaca
     2041 agggcgcgta tgcctgagtt gaaacagcag ctatttttat accgaggtcg cgcgatgacc
     2101 gacattcgca tcaaacgcat ttatgagccg cccgctccat cggacggcaa acgaatcctt
     2161 gtggacaggt tgtggccgcg aggaatggca cgcgaggagg cgcatgtaga tgcctggatc
     2221 aaggaggtcg ccccgagcaa tgcgctgcgc cgctggtttg gccacgactc cgaaaaatgg
     2281 ggcgagttca agctgcgcta tgttgcggaa ctcaggcaca atccggcggt tgctgagctc
     2341 cgggaggcgg tgccagcaag ccaatcgatc accctgcttt ttgcggccaa ggattgcctg
     2401 cacaacaatg ccatcgtctt gcgcgatttc ttgtgcgccg gtaaggcgga accctaaagc
     2461 ctatttcctt tcccgcaggg cggcccagaa tcggcgctgg tccgagcggc gaagaacgtc
     2521 gaatggaacg gtgcgatcaa tcttctccgc atcaagcagg cgaacgagat catcggcgcc
     2581 ggtaatttct ttcccatcga cggcaagcaa aatatcgccg gtgaggaggc ccgcgtgatc
     2641 ggccgggctt cccgcttcga ccgcgctcac cacggcgccc atcgcctggg tgaggccaag
     2701 ccgcagcgca attctgcgcg gcaggggaac cgtaccggcg cctataccga gataggcgcg
     2761 gcggaccctt ccatgggcga taagttctcc aagcacgaaa ctcgctgtgt tcgaggctac
     2821 cgcaaagcag attccctgcg caggcgaaat gattgccgtg ttgatgccaa tgacgtcgcc
     2881 atgcgccgag acaagcgggc cgccggaatt gccgggattg agcgcggcgt cggtttggat
     2941 cacatcgtcg attaaccggc cattcttcga gcgcaaggag cggcccagcg ccgaaatgac
     3001 tccggcggtg acacttgcgt caaaaccgaa ggggttaccg atcgcgatcg cgatctcccc
     3061 cggcttcagc ttcttcgagt tgccaagcct tgccgcagga aggattgcgt tttcgtcgat
     3121 gcgcacaagg gccaaatcgg tgtctgggtc gtcaccgagg acgcggccgg aaaaaatgcg
     3181 cccgtcgagc gccgtcacct cggcccgctt ggcgccttgc acgacatggc tgttggtgag
     3241 gacaaaaccg tccggcgaga gaatgacacc ggagccagac ccacttcgtc cgcggccgcc
     3301 ggaggcaata gcgagccgca cgacgcaagg cccaacccgc ccaactgccc ctgtcaccgc
     3361 ctccgaataa gcatccagaa gcctatgatc gccctcttcc gcggcaacgt atgcggcaga
     3421 acgatcggca tctatttcac cacacaggcc accgcctgcc tccgcttccg ccacgcatcc
     3481 aagaacattc tgatccatgc ccaaaatatg ggacgcgggg cggccaaaat aagagcccgc
     3541 ttgctctcgg cgtaccggac tagagcgttg cgaaaaacct ttgtgggaac ggccgccgcg
     3601 ccaacgattc cctcgctctg gcgccatcag ctagcagagg actagggcta cctaatttcg
     3661 tggcttcgtc ttgcgccgcg cggcgctgaa aaaaaacgaa aaatacccgt gccgaccaac
     3721 caggaatgct caagccgcat ccggccggtg ttcagcggcc gatcttgcaa agttgacatt
     3781 atcgaggaat tggcgcgcgc tatttgccca tgtgaaagtc agcgcatgtg ctctcgcctc
     3841 ttcgcgcgga atttcgagtg ccgcgagaca cgccgcgcgc tgatccgtgt cgagaacgcc
     3901 ggcgccgctc gtgccgataa catcgagggg gccaggcacc ggcagagccg ctaccggcaa
     3961 gccgcaagcg agcgcttcaa tgaggacaat tccgaaggtg tcggtgaggc ttgggaaaac
     4021 gaacacatcc gacgaagcat agtaaacggc gagcgcatcg gggtcttgca tgccgataaa
     4081 atgcgcttgc ggatagctcg cctgcagcgc gcggcgcgag ggaccatctc cggcaatcaa
     4141 tttcgatccg ggcaaatcaa gcgatagaaa tgcttcgaga tttttctccg gtgcgagccg
     4201 ccctgcgtaa agaaagatag gccggggcag atcgagcatt tttgctttcg atggattgaa
     4261 ttgactgttg tcaacgccgc gggaccagcg catcaggcgc tgaaatccgt ggctctttag
     4321 atcgtccgcg atagatttgg tcgataccat tatgccgtaa ccggcgttat gaaaccggcg
     4381 cagcagcgca taagtgattt cttccggtat tctggtgcgg gcggtgatat attccggaaa
     4441 cctcgtgtga tagctcgtcg tgaagacgcg tttcgtccgc aggcaatagc ggcgcgcggc
     4501 aaggcccagc ggaccctcgg tagcgatgtg gatatggtct gctgccgcgg cgtcgagccg
     4561 cttggcgacg gcccaaggac ttgccaaagc gagtctgatg tcgtcgtagg tcgggagcgg
     4621 aaccgtcgca aaaccttgcg gcgtcaagaa atctatgtca gttccgattt cgcgcagagc
     4681 gcgaccaaca gattcaagcg agcgcacgac gccattgacc tgcggcttcc aagcatccgt
     4741 caccaccaaa atgcgcatca cgctgccgcc ttcgcggcgc tccttgcgct atcttgttgg
     4801 gcgtcagccg cgaggcgctg ttcttcctcg gtcctctgcc agtgcaaaat ctcaagcctt
     4861 ccgtcgtgat gctcggcggc cgcggtgcag gattccacga aatccccagt gttcacatag
     4921 gtaataccgt cgatgtcgcg aattacggcg tggtggatat ggccacaaac gaccccatcg
     4981 gcatggtggc gtctcgccgc ctcagccaag gttgcctcga aagcgccaat aaagttcacc
     5041 gcattcttaa ccttaagctt ggcccattgc gacagcgacc aatagggaaa gccgaacgcc
     5101 cggcgcgcct tgttgaacca aagattagac cacagcgcca ccgtataagc ccaatcacca
     5161 agaaaggcga cccaacgcgc atgctggaca acgatatcga actgatcgcc gtggataata
     5221 agcaggcgtt tgccgtcggc ggtcacgtgg taggcgtgat cgacgacttc cacgccgcca
     5281 aacaccatac cgatataacc gcgggcaaat tcatcatgat ttcccggcag gaaaatcacg
     5341 cgggagccct tgcgtgcttt gcgcagcagc ttttgcaata cgtcattatg cgtctggggc
     5401 caataccatc cgctcttgag ccgccatccg tcgatcatgt caccgacgag gtagattgtc
     5461 gcggcatcat tatatttgag aaaatccaaa aagagttccg cctggcagcc tcttgtgccc
     5521 aaatggacat ctgagagaaa gagggtacga taaacggtcg gctctggctc gtgctcttgg
     5581 ttcaccggga aaaacctggt taaatggctg aagcgggacg aagcgaaagc agattcttat
     5641 aacttttgga tgacagagcc acaacaactc caccgtgccg tgagggggca tgacctctgt
     5701 gggaggcggg ccagccctct gtcaccggct cgtgcacaac gaagcatctt tttgcggaat
     5761 tggctattat ttccggcgtg gagtgaaggc ggcgagctcg atcaatggat gtcgtggggc
     5821 gtgataaggc gaaggcgctg gaaatcggag ctgctgccgt ctcgagtcag cttgggaaga
     5881 cggtccagcg gctgcgcaag gcttataatc ttacgctttc ggaactttcg gaacaatctg
     5941 gggttgcaaa atccatcata agccagatcg agcgcaacga gaccaatccg acactcgcga
     6001 cgatttggcg tctctcgcag gcgctcgaca tatcaatcga acgggtgctg aaggcggccg
     6061 acgacgagcc ctttttggaa aaaacctcgc gcggcgaaac gccaattctg gtgtcagatg
     6121 acggcaagtg taagctttcc atcattggct ggatcaagac ggtcgaatgg gtgcagtggt
     6181 atggtttttc agctgagcct ggcggcgttc tcgaatccga ggcgcatcag cgcggctctg
     6241 tcgaaagctt gtcggtaagc gcaggagaac tcgaagttga ggtgggcggt gcgaccgagc
     6301 gagccaaggc cggcgaaacg ctcaggtatc gctgcgaccg tccgcacacg atccgcaaca
     6361 tatcgcagga acctgcttca gcgacgatgg tctgcattct caaggcagcg gcgatgcaat
     6421 agcgcggtcc acgcatttct ccttaccgcc gtaatgggtt tgcgcggatc ggaaaaaagt
     6481 gtttcacatg aaacattttt gtccgattgg gaccaaaaac ctta
//
LOCUS       DATMPU010000262         2356 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_2680406,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000262 DATMPU010000000
VERSION     DATMPU010000262.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 2356)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2356)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2356
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2680406"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1184
                     /locus_tag="VNY06_06045"
     CDS             <1..1184
                     /locus_tag="VNY06_06045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012590370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HWX14403.1"
                     /translation="VEALQGTTWAAIAWLLGTSGDPIAQSFVLVLFSLAAAMTATISA
                     SIPAAVTGAVTPTTLATLAFLSSPSLSGAALPLASLARGTQLSFMLLARKLYKASLET
                     LSFQSEKDELIAELEQARVHSDLARHRAEAANLAKFRFLATMSHELRTPLNAIVGFSE
                     VMKGELFGAHTVESYKEYSNDIHASGQHLLMLINEILDLSRIEAGQFELKEEAIALAH
                     IMSYCRRLLRLRAKKREISVEQAVEPDLPRLWVDERAIRQVILNVLSNAIKFTRQGGT
                     IKIKIGWTASGGQYVAVRDSGPGIPEDEIPIIMSYFGRGSHAQTHAEEGSGLGLPIAK
                     GLVELHGGSFTLKSKLREGTEVIVIFPPSRIEGAVAEANGNERGFRTAQWVGHKSTKA
                     A"
     gene            1335..>2356
                     /locus_tag="VNY06_06050"
     CDS             1335..>2356
                     /locus_tag="VNY06_06050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020176171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase S10"
                     /protein_id="HWX14404.1"
                     /translation="MKDHSRLRAPLKEFWLGILAASILFPGPISSSAALEPTAAEPAA
                     VQGLPQSSAPSTPGPAVEATPNPLPPAVTSSHTMELPSRSLHFKAIAGPVRLTDAQSG
                     KPQADVGATAFLLEDTDPAKRPVTFAINGGPGAASAWLDLGSLGPWRLPFERAMFSPS
                     ASPITIDNADTWLDFTDLVFIDPPETGYSRLLTKDNEARRHFFSVTGDIEALAVTIRK
                     WLAANSRLESPKFIVGESYGGFRAPRLARRLQDVEGIGITGLVLVSPVLDFNWWSDGV
                     ANPLACAARLPSQVAAARGLSGQEARSSLADVETYAAGPYITDLLRGERDPQALARIA
                     EKIASFT"
BASE COUNT          483 a          703 c          652 g          518 t
ORIGIN      
        1 tcgtggaagc acttcaggga acgacttggg cggcgattgc ctggctccta ggtacgtctg
       61 gagaccccat cgcgcaaagc tttgtgcttg tcctattctc ccttgccgct gccatgaccg
      121 cgacgatttc ggcctcaatt cctgcagcgg tgacgggagc tgtgacgccg acgactctgg
      181 caaccctcgc tttcctgagc tcgccaagcc tttccggtgc agctttgccg ctggcgtcat
      241 tggcccgtgg cacgcaactc tccttcatgc tccttgcccg aaagctttat aaagcctctc
      301 tcgagaccct ctccttccaa tccgaaaagg acgaactcat cgccgagctc gagcaagcca
      361 gggtgcacag cgatcttgcc aggcaccggg cggaggccgc gaacctcgcc aagttccgct
      421 ttctcgccac gatgagtcat gaattgcgta caccgctcaa tgccatcgtt ggattttctg
      481 aggtcatgaa gggcgaattg ttcggcgcac atacggtcga atcctacaag gaatattcaa
      541 acgacatcca tgccagcggc cagcatctct taatgctgat caacgagatc cttgatcttt
      601 cgagaataga ggcgggacaa ttcgagctta aggaagaagc aatcgcactt gcccatatta
      661 tgagctattg ccggcgcctt ttgagattac gtgcgaaaaa acgcgagatc tccgtcgagc
      721 aggcggtgga gccagatttg ccaaggcttt gggtcgacga acgcgcaatt cgccaagtga
      781 ttttgaatgt tctctccaat gcaatcaaat tcactcggca aggcgggacc atcaagataa
      841 agatcggttg gacggcaagc ggcggtcaat atgtggcggt gcgtgatagc ggtcctggaa
      901 ttcccgaaga tgagattcca atcattatgt cctattttgg ccgtggctcg catgcacaga
      961 ctcatgctga ggaaggttcg ggccttggac tgccgattgc gaaaggtctc gttgagcttc
     1021 acggcggatc cttcacgctg aaatccaagc ttcgcgaagg tactgaagtc attgtgattt
     1081 ttccgcccag ccggatcgag ggtgcagtgg cagaggcgaa cggaaatgaa aggggctttc
     1141 ggacagcaca atgggtgggg cacaaatcta caaaggctgc gtgatcttaa tttgaaccac
     1201 gccgttccaa gccgaaaaaa ccttgccttt tcttaaacag actgcaaatt gcggcggcat
     1261 ttgatcgtgc gcagatctgc gggctctctg acgcaccccg ccaaagttga ttcttgggga
     1321 gtcggacaag ccaaatgaag gatcattcac gattgcgtgc tcctcttaag gaattttggc
     1381 tcgggatcct cgcggcaagc atcctttttc ccggccctat ttcgtcctcc gctgcgcttg
     1441 agccgaccgc cgcagaaccc gcggcagtgc aagggctgcc gcaaagttcc gccccgtcaa
     1501 cgccgggccc agccgtagaa gcgacgccca atcctctgcc ccccgctgtt acgtcctccc
     1561 atacaatgga gcttccctca cgcagcctgc actttaaggc gattgccggt ccggttcgat
     1621 taaccgatgc gcagagcggg aagcctcaag ccgatgtcgg agccacagca tttcttctcg
     1681 aagatacgga ccccgccaaa cgccctgtca cttttgcgat caatggcggc cctggcgccg
     1741 cctcggcatg gctcgatctc gggagcctcg ggccatggcg gctgccgttt gagcgcgcga
     1801 tgttttcgcc ctcggcttct cccatcacga tcgacaatgc cgacacctgg ctcgatttca
     1861 cagacctcgt ttttatcgat ccgccagaga cgggctatag ccgccttcta actaaggata
     1921 acgaagcgcg gcgtcatttt ttttcggtca ccggcgacat agaggctttg gcagttacca
     1981 tccgcaagtg gctcgccgcc aacagcaggc ttgaaagccc caagttcatc gttggcgaga
     2041 gctacggcgg ctttcgcgcg ccaaggctcg cgcgccggct gcaggatgtg gaaggtattg
     2101 gcatcacggg cttggtgctg gtctcgccgg tgcttgattt taactggtgg tcggatggcg
     2161 tcgcaaaccc cctcgcttgc gccgcccgcc tgccgtcgca agtcgccgcc gcgcgcgggc
     2221 tttcgggaca agaggcccgc agctctcttg ccgatgtgga gacctatgca gcgggcccct
     2281 acatcactga tctcctgcgc ggagagcgcg atcctcaggc actcgcgcgt atcgccgaaa
     2341 agatcgcaag tttcac
//
LOCUS       DATMPU010000263         3224 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_3305642,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000263 DATMPU010000000
VERSION     DATMPU010000263.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 3224)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3224)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3224
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3305642"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            86..700
                     /gene="phaR"
                     /locus_tag="VNY06_06055"
     CDS             86..700
                     /gene="phaR"
                     /locus_tag="VNY06_06055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012591975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyhydroxyalkanoate synthesis repressor PhaR"
                     /protein_id="HWX14405.1"
                     /translation="MAKENSPITIKKYANRRLYNTDTSAYITLDDLAAIVKRGDDFVV
                     YDAKTGEDITRSVLTQIIFEQEGKGGQSLLPITFLRQLIRFYGDSMQMLVPSYLEFTI
                     GRFASEQAKFREKIAKNFGNPLTTPIFESIEEQARKNMALFEQALTIFSPFTKPHQTP
                     AEKPSEAPAEQAPAEPGAHDIEEMRRQLDLLQKRIESLGSKESK"
     gene            complement(692..1159)
                     /locus_tag="VNY06_06060"
     CDS             complement(692..1159)
                     /locus_tag="VNY06_06060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008195422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HWX14406.1"
                     /translation="MAVDSISGLSAAEIIRLLDLHPHPEGGHFRETFRDPVQDARGRA
                     VSTLIYFLLSTGEVSRWHKVDATEVWHYYGGAALELKIFQRGSEISVHRLGLDLLARE
                     RPQFVVPAHWWQSAMSLGPWTLLGCTVAPGFECSGFELAPLHWSPGEPRETLL"
     gene            complement(1200..1970)
                     /gene="gloB"
                     /locus_tag="VNY06_06065"
     CDS             complement(1200..1970)
                     /gene="gloB"
                     /locus_tag="VNY06_06065"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002713681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004416 - hydroxyacylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019243 - methylglyoxal catabolic process
                     to D-lactate via S-lactoyl-glutathione [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HWX14407.1"
                     /translation="MAADIHQFLCLKDNFGVLVHDPATGATAAVDAPEAAPILAALTA
                     KHWELTDVLVTHHHFDHVQGLAGLKAVFPRARIVGPAKEAGRIGGLELAVGEGDSIAI
                     GALDTRAIEVPGHTSGHVAYWFASEGVLFAGDTLFAMGCGRGFEEPLEVLYQSVMKLA
                     ALPGETLVYCGHEYTLANARFALRVDTSNEKLRERAREVALRRERGEFTLPTTIAIER
                     ATNPFLRADDPSIQATLGRKGADPVEVFKELRERKNRM"
     gene            2162..2950
                     /locus_tag="VNY06_06070"
     CDS             2162..2950
                     /locus_tag="VNY06_06070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012590914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HWX14408.1"
                     /translation="MPIDVTDLRSFYSSSLGEVARRAVYKIVRQRFSNCTGYSILGVG
                     YATPYLDVFREEAVRVLAFMPAEQGVMNWPLAGVSSSALVETTAIPLPNSCIDRALVV
                     HALEITEHPHQLLSEIWRILTPGGRVIVAVPSRSGLWARLDRTPFGHGRPYSRIQLGE
                     LMRNALFSPVYWTEALYIPPFQHRTLLHGANFAEEIGAALSLPWGGVLMVEATKLLYL
                     PIGARRTAAYGVAVTSGSPVFVPEAAGASPVVAPLVARNGEFCP"
     gene            complement(2962..>3224)
                     /locus_tag="VNY06_06075"
     CDS             complement(2962..>3224)
                     /locus_tag="VNY06_06075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012590915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HWX14409.1"
                     /translation="QEEGTPPISAGATPPILLVHGSQDDVVPMEALFLSAEKLAEAGI
                     PCQWHLTIGLGHGIDSQGLMHGGLFLAKCFGLRPDPSAQARG"
BASE COUNT          682 a          924 c          925 g          693 t
ORIGIN      
        1 gaaagttgtc tcacccttag ccgtggcgtg ggcaatccaa atctgattag gttcgctcga
       61 gagtataatc aagcgggatg agggcatggc caaagagaat agtcccataa ccatcaagaa
      121 atacgccaac cggaggctct ataacaccga taccagcgcc tatatcacgc ttgacgacct
      181 cgccgcgatc gtcaagcgag gcgacgattt cgtcgtttat gacgctaaga ccggagagga
      241 cataacgcgt tcggtcctca cgcaaattat ctttgaacag gaggggaagg gcggacagag
      301 cctcctgccg attacgtttt tgcgccagct catccgattt tatggcgaca gcatgcaaat
      361 gctggtgccg agctatctcg aattcaccat cggcagattc gccagcgagc aagcgaaatt
      421 ccgcgagaaa atcgctaaga actttggcaa cccgctgacg acaccaattt ttgaatcgat
      481 cgaggaacag gcgcgtaaga acatggctct gtttgagcag gctcttacga tcttcagtcc
      541 gttcacaaag ccgcaccaaa caccggcgga gaagccctct gaagcacccg ccgagcaagc
      601 tccagcagag cctggtgcac atgatattga ggaaatgagg cgacagctcg atctgctgca
      661 aaagcggatc gaatcgctag ggtccaagga gtcaaagtaa ggtctcgcga ggctcgccag
      721 gtgaccaatg aagcggcgca agctcgaaac ctgaacattc gaagcccggc gcaacagtgc
      781 agccaaggag cgtccagggt ccgaggctca tcgcgctttg ccaccaatgg gccgggacga
      841 caaattgcgg gcgctctcga gcaagaagat caaggccaag ccgatgcacg cttatttcgc
      901 ttccgcgttg aaaaatcttt agttccaacg ccgctccgcc atagtagtgc cagacctcgg
      961 tggcatcgac tttgtgccag cgcgacacct caccagtgct cagcagaaag taaatcaacg
     1021 ttgagaccgc gcggccccgc gcatcttgta ccggatcgcg aaacgtttcg cgaaaatgcc
     1081 caccctcggg gtggggatgc aaatcgagaa ggcggataat ctcggcggcg ctcaggccag
     1141 agattgaatc aacagccatg ggtttcgcgc tggcggccaa gctaaggtgg agcgggtgat
     1201 cacatccggt tctttcgttc gcgcaactcc ttgaagactt cgaccgggtc ggcgcccttt
     1261 ctgccgagcg ttgcctggat tgacgggtca tcggcgcgca aaaaaggatt tgtcgcacgt
     1321 tcaatcgcga tcgtggtcgg aagggtaaat tctcctcttt cgcgccggag agccacctca
     1381 cgggcgcgct ccctcaactt ttcgttggag gtatcgaccc ttaaggcaaa gcgggcgttc
     1441 gcgagtgtgt actcatggcc gcaatagacc agcgtttcgc ctgggagggc ggcgagtttc
     1501 atgacagatt gatagagcac ctcgagaggc tcctcaaagc cgcggccgca tcccatcgca
     1561 aacagagtgt ctcccgcaaa aagcacgcct tcggatgcaa accaatatgc gacatgcccc
     1621 gaggtatggc ccggaacctc tatagctctg gtgtcgagcg cgccgatggc aatgctgtca
     1681 ccttcgccaa ccgcaagttc aaggccgccg atcctgcctg cctcctttgc tggccccacg
     1741 atacgggcgc gcggaaacac cgctttgaga ccggcgagcc cttggacatg gtcgaaatgg
     1801 tggtgcgtga ccaagacgtc ggtcagctcc caatgtttgg ccgtgagcgc ggccaggatt
     1861 ggcgcggcct cgggcgcgtc gaccgcggcg gtcgcgcccg ttgcgggatc atggacgagc
     1921 acgccaaaat tatcctttag gcacaggaat tggtggatat cagccgccat gccaacgtgc
     1981 gccttcgatt gattgcagcg ttcgcttcga tattttaagg gccagcacga ttgaccgctt
     2041 ttcggcggga ttgatttttt gctttggccc tcttctttgg gagtcaagac tttatttttg
     2101 cgctccgcaa gcagattatc tgttgcccgg tgttcttttt tagggtccgg aagaattccc
     2161 catgcccatc gatgtcaccg acctgcgctc tttttatagc tcgtcgctcg gagaggtcgc
     2221 acggcgtgct gtttacaaga tcgtgcgcca gcgattttcc aattgcacgg gatattcaat
     2281 tctgggtgtt ggctacgcga cgccctatct tgatgtgttc cgcgaggaag cggtgcgcgt
     2341 gcttgcgttc atgccagccg aacaaggtgt catgaattgg cctttagccg gcgtttcctc
     2401 ttcggcgctt gtcgaaacga cggcgatacc tttgccgaat tcctgcatcg accgggcgct
     2461 cgtcgtgcac gcgctcgaaa tcaccgagca cccgcatcag cttctttcgg agatatggcg
     2521 gatcttgacc ccgggcggcc gggtgatcgt ggccgtgccg agccgttccg gcctttgggc
     2581 acggctcgac cggacgcctt ttggtcatgg ccggccttat tcgcgcattc aattgggcga
     2641 actcatgcgc aatgctttgt tttctccagt gtattggaca gaagcacttt atataccacc
     2701 cttccaacac cggactctgc tgcacggcgc caattttgcc gaagaaatcg gcgctgctct
     2761 ttcgcttccg tggggcggag tattgatggt cgaggcaacc aaactccttt atttgccgat
     2821 cggtgcaagg aggactgcgg cctatggcgt agctgtgaca agcgggtctc ccgtcttcgt
     2881 gccggaagcg gctggcgcct cgcccgtggt tgcgccgctg gttgcgcgca acggggaatt
     2941 ttgtccgtaa gcgagcgccg ttcaaccccg cgcctgggcg cttggatccg gccttaggcc
     3001 aaagcatttg gccagaaaca gaccgccgtg catgagccct tggctgtcga tcccatgacc
     3061 gagaccaatc gtgagatgcc actggcaagg aataccggcc tccgccaatt tctcagccga
     3121 aagaaaaagc gcttccatcg gaacgacgtc atcttgactg ccatgaacaa gtagaattgg
     3181 aggggtggcg ccggcggata tagggggcgt tccttcctct tgcc
//
LOCUS       DATMPU010000264         2593 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_3306235,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000264 DATMPU010000000
VERSION     DATMPU010000264.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 2593)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2593)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2593
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3306235"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..899
                     /locus_tag="VNY06_06080"
     CDS             <1..899
                     /locus_tag="VNY06_06080"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012332712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HWX14410.1"
                     /translation="RQIQWVSAGLLRDLWRIEFKTDARAAFRGIDGFGLWESRTGLYF
                     FDPPLEGDRDFYAQFYSKLTGLGLYSQTYIRHEFTMAARSIPAGARVLDVGSGLASFR
                     SCLLPKANYTGLDPHIADKSKAAEIRGETLAQHLIEHAGTYDAVCAFQVLEHVTAPAI
                     LFAEMVQAVRPGGQIFAGVPHVPSAMTRIPNFIINAPPHHLTWWTKPALAELAKGAGA
                     IVESIENVPWSSSDALIYWIERCTLKKCNGEHYRGTWTWHASAALGLIGGMIANPLAG
                     VPSGGGEGSGLLLTARRAMDAQ"
     gene            924..2219
                     /locus_tag="VNY06_06085"
     CDS             924..2219
                     /locus_tag="VNY06_06085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012591717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HWX14411.1"
                     /translation="MEDWRRSDVEASLDNLVRESRSLPTMSVIVSTVLSVWIAFALLN
                     TFTAWKYARGLPFAEVPQATLSAAVIVAIKGTSDISRAFFTRLRNQAYPDYRIIAAIE
                     SELDPAFAMLMEEMSRTGAPLRIVIAGQSTNTGQKVWNLLAALDALEDRDELVVFVDA
                     DTLPAPEWLPRLVASLINPGREAVTGYRWIIPADNRLSSAVVAAANASIVTTPRLPSI
                     INHCWGGTMAMRRKTLERIDIRRYWTGAISDDAQMTRAFKQAGCPVFSPRQSLLLSPI
                     SMSWKEAFLFGRRQYSILWTYTRVLWILAALGTAIPIAAALAAMSLALQGNVFALAAI
                     IVALILGEARYRCRRKIFAALWGAEDIGEKGLYWRVERWLRPLWWSFHALCVFSALGS
                     RRIHWAGVDYWIRGPQDVDVSRPQVQRRQDDHLNRESLD"
     gene            complement(2286..>2593)
                     /locus_tag="VNY06_06090"
     CDS             complement(2286..>2593)
                     /locus_tag="VNY06_06090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020173457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HWX14412.1"
                     /translation="DEGEETRLGDLAELVDVKIAGIRQRFGALGDQRLSIMAAITLAD
                     ERAEANDRIRELEAEVARLKMSTLGAPEWAEQLATSLCEAAQRIKTVAQELSDSSRI"
BASE COUNT          515 a          772 c          716 g          590 t
ORIGIN      
        1 tccggcagat ccaatgggtg tcggcggggc tcctcagaga tctttggcgc attgagttca
       61 agactgacgc tcgagcagcc ttccgaggta tcgatggatt cggattatgg gagtcacgaa
      121 caggtcttta tttcttcgat cccccgctcg aaggagaccg cgacttttac gcccagttct
      181 attcaaaact cacaggccta ggtctctatt cgcaaacata cattcgccac gaattcacga
      241 tggcggcaag gtccattcca gctggcgctc gcgtgcttga tgtcggtagt gggttggcaa
      301 gtttcaggtc atgcctcctg ccaaaagcta attacaccgg gcttgatcca cacatcgccg
      361 acaagagcaa agccgccgaa atccgcggcg aaacacttgc ccagcatctt atcgagcatg
      421 cagggactta tgacgcggtg tgtgccttcc aggtcctcga acatgtgacc gcaccagcga
      481 ttctgttcgc tgagatggtg caagcggtga ggccgggcgg ccaaatattt gccggcgtgc
      541 ctcatgtccc atcagcgatg acccgcattc cgaattttat catcaacgcg ccaccccatc
      601 acctgacatg gtggaccaaa cccgctctcg ccgaactggc gaagggagcg ggcgctattg
      661 ttgagagcat cgaaaacgtt ccatggagct ccagcgatgc gctcatttat tggatcgaac
      721 gctgcaccct aaaaaagtgc aatggcgagc actatcgtgg aacttggacc tggcacgcct
      781 ctgccgcgct tggcctcatc ggcggcatga ttgctaaccc gctcgccggc gtgccttccg
      841 gcggcggcga aggctcggga ctattgctga cggcgcgccg ggcaatggat gcgcagtaag
      901 aggtttttat ctactctctg ccaatggaag attggcgtcg ctccgacgtg gaggctagtc
      961 tcgataatct ggtacgagaa agccgcagcc tacccacaat gagtgtcatc gtttccactg
     1021 ttttgtcagt ctggatcgcg tttgcgctcc tcaatacgtt tacggcgtgg aaatatgcgc
     1081 gcggccttcc cttcgccgaa gttcctcaag cgacgctttc cgccgccgtc attgtcgcga
     1141 ttaaagggac ctcggacatc tcgcgcgctt tcttcaccag gctgcgcaat caagcctatc
     1201 ccgactatcg aatcatagcg gcaatcgagt cggagcttga tccggccttc gccatgctca
     1261 tggaagaaat gagccggact ggagcccctc ttcgcattgt catcgccggt caatcgacca
     1321 ataccggcca aaaggtctgg aatttgcttg ccgcgctcga cgccctcgag gaccgtgatg
     1381 agctcgtcgt ttttgtcgat gcagatacgc tccctgcccc tgagtggctg ccgcgtctcg
     1441 tcgcaagcct catcaacccg gggcgggagg cggtcacggg ttaccgctgg attattcccg
     1501 ccgacaatcg gctgagttct gccgtcgtcg cggcggcaaa tgcttcgatc gtaacgacgc
     1561 caagacttcc ttcaatcatt aaccactgct ggggtggcac gatggccatg cggcgaaaga
     1621 cgcttgagcg tatcgatatt cgccgctact ggaccggagc gatcagcgat gacgcacaaa
     1681 tgacacgcgc cttcaaacaa gcgggctgcc cggtcttttc gccgcgccaa agcctcctcc
     1741 tttcccctat atccatgagt tggaaagagg cttttttgtt cgggaggcgc caatacagca
     1801 tcctttggac ctacacacgg gttctttgga ttttggcagc gcttggcacg gctatcccga
     1861 ttgcagcagc gctagccgct atgagcctag ccttgcaggg aaatgtcttt gccctcgcgg
     1921 ccatcatcgt agctctaatc ttgggcgaag ctcgttaccg ctgccgacgc aaaattttcg
     1981 ccgcgttatg gggtgcggaa gacatcggcg agaaaggatt gtactggcga gtcgaacgtt
     2041 ggctcagacc cctttggtgg agttttcacg ctctttgtgt tttttccgct cttggctcgc
     2101 gccgcattca ctgggctggc gtcgattatt ggatacgcgg gcctcaagat gtcgatgtgt
     2161 cgcgtccgca ggtgcaaaga cggcaagacg atcaccttaa tcgcgaaagc ctggattaaa
     2221 atatccgtcg ctgccgttgc gatcgctagg caatgcatga cgaaaccaag cccgagaaag
     2281 ccgcctcata tgcggctcga gtcgcttaac tcctgcgcca ccgttttgat tcgttgcgcc
     2341 gcctcgcaga gtgaggtcgc cagctgctcg gcccattcgg gtgcaccgag cgtagacatt
     2401 ttgagcctcg caacttcggc ctcaagctcg cggatccgat cgtttgcttc cgcccgttca
     2461 tcggcgaggg taatcgcggc cataatgctc aggcgctggt cgccgagcgc gccgaaccgc
     2521 tgccttatcc cggcaatctt gacatcgaca agttcggcga gatcgccgag acgagtttcc
     2581 tcgccctcat cgc
//
LOCUS       DATMPU010000265         4882 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_839113,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000265 DATMPU010000000
VERSION     DATMPU010000265.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 4882)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4882)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4882
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_839113"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..194)
                     /locus_tag="VNY06_06095"
                     /pseudo
     CDS             complement(1..194)
                     /locus_tag="VNY06_06095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008876559.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1772 domain-containing protein"
     gene            358..1560
                     /gene="lhgO"
                     /locus_tag="VNY06_06100"
     CDS             358..1560
                     /gene="lhgO"
                     /locus_tag="VNY06_06100"
                     /EC_number="1.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013530256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-hydroxyglutarate oxidase"
                     /protein_id="HWX14413.1"
                     /translation="MTYDFAIIGAGIVGLAAAREILHARPSSSIIVLEKESTVAQHQT
                     GHNSGVIHAGIYYAPGSLKARFCREGAVATKAFCVEQGIKFETCGKLLVATNESERLR
                     MDALFERAKQNGVEAEQIDAAELSHLEPNITGVGALLIPSTAIVDYKTVASAFAQTVT
                     AQGAELRLCAEVIAIREGSDAVEISTAGQSWRGRFMIVCGGLQSDRLAKLAGLKIGHR
                     IVPFRGEFYTLPKSRAGLIHRLIYPIPDPKLPFVGIHLTRTIDGRIIAGPNAVLGFAR
                     EGYAKFSVNPRDVASFLAFPGFWRVAGHNLATGMAEIRNALWKRGYLAQCRKYCPDLS
                     LADLEPGVAGIRAQAVMRDGTMMHDFLFEETARMLHVCNAPSPAATSAIPIGRMVAAK
                     ALRQFRQR"
     gene            1641..2231
                     /locus_tag="VNY06_06105"
     CDS             1641..2231
                     /locus_tag="VNY06_06105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX14414.1"
                     /translation="MIGIGRFAAALTLGFALWGTKAQAQDTSLMDMNMNMGCMLMAGM
                     HELQVAVYQSGALDDSCEDLPGPGPAVVTLSSASKELRDLTTEVRIVRGDTGEAASGT
                     SLDPITLVYAPPKAYPTGVITLNTNFDKAGKYTVLATVRDDKDMVMSGHLGITVGAGS
                     RQVIYAFLASGVILAAALGYYLWEHRRKSKMSFKGS"
     gene            complement(2295..3083)
                     /locus_tag="VNY06_06110"
     CDS             complement(2295..3083)
                     /locus_tag="VNY06_06110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007066814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003796 - lysozyme activity [Evidence
                     IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA];
                     GO_process: GO:0016998 - cell wall macromolecule catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH25 family lysozyme"
                     /protein_id="HWX14415.1"
                     /translation="MQHFFRKLFAFGTNWWLGVALLAGGCASSITGDDILPRPGDYEV
                     GGIDVSRYQGDIDWSSVRASGVRFAWIKATEGGDRIDDKFAQNWAAAKAAGVPRGAYH
                     FGYWCRPMQEQAAWFKAHVPNDPAALPPVLDVEWNGNSKTCPKRVPRDFAIAQMKVML
                     AAMEEAYGKKPVIYTSIDFHRDVMQGEFTDYPVWVRSVTCHPSLKYGTRRWHFWQHTA
                     EGHITGIRGNVDRNAFNGSLKDWQAWLANNKSLAGNKSPSEHGA"
     gene            3370..4882
                     /locus_tag="VNY06_06115"
                     /pseudo
     CDS             3370..4882
                     /locus_tag="VNY06_06115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012589733.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
BASE COUNT         1003 a         1408 c         1316 g         1155 t
ORIGIN      
        1 cgacgcccaa gataaagcca atgagcgcga aggaagcctg catcgcaaag ccgcgcttgt
       61 aggcaggttt ccattctgca agcagcccgc ggtcgtcaag tctaagcctc gccggctgct
      121 ctgctacatt gatgtagatc ggcggcaccg gtaaaaagag acgcgatgac gagggcgagt
      181 tctccagcaa gcatgcctat ttctcccttg aaatcaagta tatgccaatt tgttgtcgca
      241 gtcttgcgtc tccagctgta agccatctat tagattgcag ccactcggaa tggaaatgtc
      301 cacggcgaag gaggtttgcc gcaattaaac tccgtatgcg tgattgccta tctccgaatg
      361 acttatgatt ttgcaatcat tggcgctggt atcgtcggcc tcgcggccgc acgggaaatt
      421 ctgcacgcgc gccccagctc ctcgatcatt gttcttgaaa aggaaagcac tgtcgcacaa
      481 caccaaaccg ggcataacag cggggtcatc catgccggta tctattatgc gccaggaagt
      541 ttgaaggcgc gcttttgccg cgagggtgct gtggccacaa aggctttttg cgtggagcag
      601 ggcataaaat tcgaaacctg cggcaagctt ctcgtagcta cgaatgagtc cgagcggctg
      661 cggatggatg ccttgttcga gcgcgccaag caaaacggcg tcgaagctga gcaaatcgac
      721 gcagccgagc tcagccacct cgaaccaaac attacgggcg ttggcgcgct tttgatcccg
      781 tcgactgcga tcgtcgacta caaaacggtc gcctccgctt ttgcccaaac cgttacggca
      841 cagggtgcgg aactacggct ctgcgccgaa gtcattgcca ttcgcgaagg ctccgacgcc
      901 gtcgaaatct cgactgccgg gcaaagctgg cgcgggcgct ttatgattgt ctgcggcggg
      961 ttgcagtccg atcgtctcgc gaaattggct ggcctgaaga tcgggcaccg gatcgtacca
     1021 ttccgaggcg agttctatac tttgccaaaa tcaagggctg ggctgatcca tcgcctgatt
     1081 tatcccatcc ccgacccaaa actccccttt gtggggattc acctcacgag gacaatcgac
     1141 ggtcgaatta tcgctggtcc caacgcggtg cttgggtttg cccgcgaggg ctacgcgaaa
     1201 ttttctgtca atcccaggga tgtcgcgagc tttttggcgt ttccgggctt ttggcgtgta
     1261 gcaggacaca atctcgcaac gggcatggcg gaaatccgga acgctctatg gaaacgtggc
     1321 taccttgcgc aatgccgaaa atattgcccc gatctttccc tcgccgatct cgagccggga
     1381 gttgctggca tacgcgccca ggccgtgatg cgcgacggaa ccatgatgca tgactttctc
     1441 ttcgaggaaa ccgctaggat gctgcatgtt tgcaacgcac cgtcgccggc ggcaacatcg
     1501 gcaattccga tcgggcgcat ggtcgccgcg aaagctctga gacaattcag gcagcgataa
     1561 aatggccgat tggcaaaacg cgcacctttc agtacaacgc cgccgcgtga cacgtagcca
     1621 gtcagggagg aaatagacca atgattggga ttggccggtt tgcggcagcc ctgaccttgg
     1681 gatttgcgct ctgggggacg aaggcgcagg cccaggatac cagtctcatg gacatgaaca
     1741 tgaacatggg ctgcatgctg atggcaggaa tgcatgagct gcaggtggcg gtttatcaat
     1801 ctggcgccct cgatgattcc tgcgaagacc tccctggtcc cggtcctgcc gtcgtcacgc
     1861 tcagctcggc atcgaaggaa cttcgggatt tgacaaccga ggttcgtatt gtacggggcg
     1921 acaccggaga ggcagcgtcg ggcactagtc tcgatccgat cacacttgtt tacgccccgc
     1981 caaaggccta tccaacaggg gtcatcacgc tcaacacgaa tttcgacaaa gctggcaaat
     2041 acacggtcct tgccaccgta agggacgaca aggatatggt catgtccgga catcttggca
     2101 tcaccgtcgg ggcggggtcg cgacaagtaa tctacgcttt tctggcttcc ggcgtcattt
     2161 tggcagcggc gcttggatat tatctttggg agcatcggcg aaaatcaaag atgtccttta
     2221 agggcagctg aggaagcttc ccgcgaagag cgcaaccctg gcagccttcc ctccctgttt
     2281 ccggtcccaa gcgattaagc accatgctcg cttggtgatt tgttaccggc gagcgatttg
     2341 ttattggcga gccaagcctg ccaatccttg agcgaaccat taaacgcgtt gcgatcgacg
     2401 ttgccacgaa ttccggtgat atgaccttca gcggtatgtt gccagaaatg ccagcggcgt
     2461 gtgccatatt tgagcgatgg gtggcaagtc acgctgcgaa cccagacggg ataatccgta
     2521 aactccccct gcatcacatc acgatggaaa tcgatcgatg tataaatcac cggctttttc
     2581 ccataggctt cttccatcgc ggccagcatg actttcatct gcgcaatggc aaaatcgcgt
     2641 ggcacgcgct tcggacaggt ctttgaattc ccattccatt cgacatccag caccggcggt
     2701 aaggcggcgg ggtcatttgg aacatgggct ttaaaccagg ccgcctgctc ttgcatcggc
     2761 ctgcaccaat agccgaaatg ataggcgccc cgcggcacgc ccgcggcctt ggccgcagcc
     2821 cagttttggg cgaatttgtc gtcaatccga tcgccgccct cagtcgcctt gatccaggca
     2881 aagcgaacac ccgacgcgcg gacgctgctc caatcgatat cgccctggta tctcgatacg
     2941 tcgataccgc caacctcata gtcgcccggc cggggcaaaa tatcgtcacc agttatgctg
     3001 ctcgcacatc ctccagccag cagagcgacg ccaagccacc aattcgtccc aaaggcgaaa
     3061 agcttccgga aaaaatgttg catcggggta gagccatttg aaaacatgag caaattaatt
     3121 cctaatccgt aatatgtcgt taaaaggtca aaaaaaggcg gtcgcacagt cacatgtttg
     3181 atgggttaat atggcgcatt tggcacaatt tagggacgga atttagggac ggccagcccg
     3241 ccgcggcaaa atgctgtcat ttgtttttca tatgcttcaa tgagtaaaat ctcgcatttt
     3301 gcgccaattc tgccctcgcc aaatagcgtc attcggccgt caggcgcaag actctcgtga
     3361 aaggccgcca tgctctcccg attcgtcgcc ttcacctcgc tgggacgcac cgcactcccc
     3421 aatcaatacg atgcttttgc gtttgcgctg attatcgcga gtttcattgc gatcacccac
     3481 gcgtcgcacg gcgttaccct tccgctggag gcgcccgaag cgactgccgt atcactcgat
     3541 tatgcgtttc tgccctatta tgcgctgcgc accgtcttac gcatgttcgc cgctatggcc
     3601 gccgcgctga tcttcacatt cgtttatgcg acgcttgccg ccaaaagccg ccgtgccggg
     3661 cttctgctca ttcccttgct cgacgttttg caatcggtgc cgatcctagg tttcctgtct
     3721 ttcaccgtaa ccttcttcct taggctcttt ccgggatcca tccttggtgc tgaatgcgcg
     3781 gcggtttttg caatttttac ctcacaggcc tggaacatgg ccttctcctt ctaccaatcg
     3841 ctgcgcaatg tgccgcgcga tctgatcgag gttgcgcggg gtttcggact ttcgagctgg
     3901 caaagatttt ggcaactcga gaccccattt gcaatgcccg gcctcatctg gaacatgatg
     3961 atgtcgatgt cgggcggctg gtttttcgtg gtcgcatccg aagccatctc ggttggcgac
     4021 acaaccatca aactgccggg catcggttct tatcttgctc ttgccatcga ggaaaagcgc
     4081 atagacgggg tatttgcggc cattatcacc atgctggcgg tcattatcgc ctacgaccag
     4141 ctgttgttcc ggccgctcgt cgccttcggg gcgcggtttc gggtcgagct ttcagccggt
     4201 caaatgcagg aaaaatcctt cgtggcgcag gtgttcgcaa ggacccattg gatgcgggcc
     4261 ttgatgcgcg gcccggtgca gctgttccaa gctatcgcgc ttctacgtat ggaactgccg
     4321 gttcgctctc gcgcgccgat ttcgcgccgc agccttgctc taagccgtct catcgatctc
     4381 ctgtggctcg ctgcaattac cctcagcacg atttgggctc tttggctcat cacagactac
     4441 attcgtcaag agctcgggtg gagcgaagtg tggaaagccg tgctctacac cctttttaca
     4501 atggtacgcg tgatcttgct catggcgctc gcgagtttgg tttgggtacc tgtcggagtt
     4561 tggatcgggc taaggcccgc gctcgcggaa attgtccagc ccctggcgca attcctggcg
     4621 gcgtttccgg cgaatgtctt atttcccgtc gccgttgttc tcatcgtcag attttccctc
     4681 aatcccgaca tctggcttag ctttctgatc atctttggca cccaatggta cattctcttt
     4741 aatgttattg cagccgcctc ggcttttccg aacgatctca agtgcagtgg cgaattttcg
     4801 cattcgcggc ttctcctggt ggaggaacgt aattattcct ggaatcttcc cctattatgt
     4861 gactggcgcg ctgaccgcat cc
//
LOCUS       DATMPU010000266         2424 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_2360512,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000266 DATMPU010000000
VERSION     DATMPU010000266.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 2424)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2424)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2424
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2360512"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            158..904
                     /locus_tag="VNY06_06120"
     CDS             158..904
                     /locus_tag="VNY06_06120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012590267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1223 domain-containing protein"
                     /protein_id="HWX14416.1"
                     /translation="MEFARPFFFALCGCVAAVATGCAIGTVVFAFAHDSYVIELFTSQ
                     GCSHCPRADRWLAAIARNPGTVAVSFPVDTWDYIGWRDTLASPAFTARQKAYAAVNGN
                     SRVYTPQAIVNGLTAAAGGDEAEIEQAIAKAKGVDGAMTVPMRLSKEDGHYSVEIAPG
                     NNGPASVIVLRVARATTVQIQRGENAGKSITYTNVVRTIDKVGEWTGNTATFSLPGAT
                     QDGEGFVVLVQKGTLERPGAILAAVKSDGL"
     gene            1260..1853
                     /locus_tag="VNY06_06125"
     CDS             1260..1853
                     /locus_tag="VNY06_06125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012590266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX14417.1"
                     /translation="MSRKAFRGFKALLPLAIVAALATTSSAVSKSKRKEAKPAPKSEN
                     APENSGTADKPAQLGMYGDWGVYFAKGEKSKTCYALATPKDRAPSGLNRDPAYIFISS
                     RPGENVREEISVIMGFPLKEDGAHAEVSGTSFALIAKGANAWIKNTAEEAHFVEALKK
                     GSKLIVKAASTKGNVTTDSYSLVGLSQALEKLENECP"
     gene            1946..>2424
                     /locus_tag="VNY06_06130"
     CDS             1946..>2424
                     /locus_tag="VNY06_06130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012590265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-coenzyme A synthetase N-terminal
                     domain-containing protein"
                     /protein_id="HWX14418.1"
                     /translation="MSGKSYPVPPSFKEQAHIDLAKYQELYAWSVENPESFWAEQGRR
                     IDWINPFTRVKNTSFGPSSVSIKWFEDGTTNVAQNCIDRHLPRLANHCAIIWEGDEPG
                     GSKQITYQELHDEVCRLANVLKDLGVGRGDTVTIYLPMIPEAVYAMLACARIGAIHTV
                     "
BASE COUNT          557 a          691 c          698 g          478 t
ORIGIN      
        1 tctgccttag aggcttggtc aataagcttg ccgggtcatc agccccataa agctattgag
       61 cttgctgcga gtggtaacca cgaattgatt ggaaccgcgt cgttgttact ctaagctgac
      121 cggcgctgta aggtcgtttt catcttggtg gccaggcatg gaattcgcac gcccgttttt
      181 cttcgcgctc tgcggatgcg tggcagcggt ggcaacaggc tgtgccattg gaacggttgt
      241 gtttgccttt gcgcatgaca gttacgttat cgagctgttc accagccaag gctgttccca
      301 ttgtccgcgc gccgaccgat ggctcgcggc aatcgcgcgc aacccgggca cagtcgccgt
      361 ttccttcccg gtcgacactt gggactacat cggctggcgg gacacgctcg catccccggc
      421 gttcactgcg cggcagaagg cctacgcggc ggtgaatggc aatagccgtg tctatacgcc
      481 gcaagccatt gtaaacgggc ttactgcggc ggcaggcggc gacgaggccg agattgaaca
      541 ggcaattgct aaggccaaag gcgtcgatgg ggcgatgacc gtgccgatgc gcctatcaaa
      601 ggaggacgga cattattcgg tggaaatcgc accaggaaac aacgggccgg cgtcggtcat
      661 cgtgctgcgg gttgctcgcg cgaccacagt acaaattcag cgtggcgaaa atgccggcaa
      721 gagcatcacg tacacaaatg tcgtgcggac gatcgacaag gtgggcgaat ggacgggaaa
      781 caccgcaacc tttagcctgc cgggcgcgac tcaagatggc gaaggttttg tcgtgctggt
      841 ccaaaagggt acactggagc gtccgggagc tattctcgcc gccgtgaagt ctgacggtct
      901 ttgagccaat aaccggggct gcggcgctcg ccgctcacgc tctgccccgg ccgccagctg
      961 ccaaaatccg ttagaaaacg tgctgagcca aagtggcaac cggttgtgcg cccagacacg
     1021 tgacaaaacg atgatttaaa gtaagatgga catcttgctg tcaacgaacc ttcttcccct
     1081 tcccaactac cctcctcact gaatatagaa gagctgatga agcagcgcca ttcccgacag
     1141 gtggcgagag cgtgtcgagc taagggttag aaccggtttt gcgtccggac acgcgacaaa
     1201 ccgaataatt actgcaagat gacgagtcca agaacagcca tcttgcacga ggagcgtcaa
     1261 tgtctcgcaa ggcttttcgc ggtttcaaag ctctccttcc gctcgccatt gtcgccgccc
     1321 tggcgaccac gagttccgcc gtctcaaaat ccaagcggaa ggaggccaag ccagcaccaa
     1381 aatcggagaa tgcacccgaa aattccggga cggccgacaa gccagcgcaa cttggcatgt
     1441 atggcgactg gggcgtttat ttcgcgaagg gtgagaaatc caagacctgc tacgctctgg
     1501 cgacgcccaa ggatagggcg ccgtccggtc taaaccgcga tcccgcctat atcttcatct
     1561 caagccggcc gggcgagaat gtgcgggaag agatctcggt catcatggga tttccgctga
     1621 aagaggacgg tgcccatgcc gaagtctcgg gaacgagctt cgctctcatc gccaaagggg
     1681 ccaatgcctg gatcaagaac acagccgaag aagcccattt cgtcgaggct ttgaaaaagg
     1741 gttcaaagct catcgtcaaa gcggcctcga caaaaggcaa tgtaacaacg gacagctatt
     1801 cgctcgttgg cctttcccag gcactcgaaa aactggaaaa tgaatgccct tgaggctcat
     1861 ctcgctgcac cggctatgag ggcgcgcggg tcttttgctg cccgcgaagg agacacgccc
     1921 gtcaagcgca ctgaggagga acaatatgtc cgggaaatcc tatccagttc ctcccagctt
     1981 caaagaacag gctcatattg atcttgcaaa ataccaggaa ctctatgcct ggtcggtcga
     2041 aaaccccgag tccttttggg ccgagcaagg gcgccgcatc gattggatca acccctttac
     2101 cagggtgaaa aataccagtt tcgggccaag ctccgtctca atcaaatggt ttgaggacgg
     2161 aaccaccaat gtcgcgcaaa actgcatcga ccggcacctt ccgcggcttg ccaaccattg
     2221 cgcgattatc tgggaaggcg acgagcccgg gggttcgaaa cagatcacct atcaggagct
     2281 gcacgacgag gtctgccgcc tcgccaatgt gctcaaggat cttggcgttg gacgcggcga
     2341 cacggtcacg atctatctgc caatgattcc ggaggcggtc tatgcgatgc tcgcctgtgc
     2401 ccggatcggc gcgatccata cggt
//
LOCUS       DATMPU010000267         4336 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_3307657,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000267 DATMPU010000000
VERSION     DATMPU010000267.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 4336)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4336)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4336
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3307657"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(225..1769)
                     /locus_tag="VNY06_06135"
     CDS             complement(225..1769)
                     /locus_tag="VNY06_06135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014495444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HWX14419.1"
                     /translation="MEALVEDLRFKAACIAQGGGVEARERHIARGKMLPRERIAALID
                     PGTPFLELSQFAAFNVYDDSVPAAGLITGIGRVSGQDCVVLANDATVKGGTYYPLTVK
                     KHLRAQEIAQENNLPCIYLVDSGGAHLPSQDEVFPDRDHFGRIFYNQARMSALGIPQI
                     AVVMGSCTAGGAYVPAMSDEAIIVAGQGTIFLAGPPLVKAATGEIVSAEELGGADVHA
                     RKSGVTDHYASNDAHALVLARNIVTNLNRAKSPGVVVREPVEPLYDAQEIYGIVPADS
                     RAAYDVHEVIARIVDGSAFDEFKALYGTTLVCGFAHIFGYPVGVLANNGILFSESALK
                     GAHFIELCAQRKIPLVFLQNITGFMVGPKYEAQGIAKDGAKLVAAVSCAKVPKFTVII
                     GGSFGAGNYGMCGRAFGPRFLWMWPNARISVMGGEQAANVLAQVRREKLEAAAKLWSA
                     EEEETFKAPVRERFERQARPYYASARLWDDGIIDPAETRMVLGLCISASLNASIEETR
                     FGVFRM"
     gene            complement(1837..2997)
                     /locus_tag="VNY06_06140"
     CDS             complement(1837..2997)
                     /locus_tag="VNY06_06140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018697646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HWX14420.1"
                     /translation="MQNPGRDFPLGPELERLRDTVYAFASAKIAPRAGEIDRSDAFPI
                     DLWPQMGALGLHGITVEEEFGGAGLGYLAHCIAMEEISRASASVGLSYGAHSNLCVNQ
                     IRRNGTEEQKRRYLPKLLSGEHVGALAMSEVGSGSDVVSMRLRANKKGGRYILNGTKM
                     WITNAHHAAMLVVYAKTDPDAGAHGITAFLIESSFRGFRLGQKLDKLGMRGSPTSELV
                     FENCEVPEENVLGAVGDGVQVLMSGLDYERVVLAGGPLGIMQACLDICLPYVRDRKQF
                     GHAIGEFELVQSKLADMYVGLTACRSYVHAVARACDNGFTSRKDAAGALLFAAEHATR
                     AALDAIQLLGGNGYINDYATGRLLRDAKLYEIGAGTSEIRRMLIGRELFDGH"
     gene            3140..4282
                     /gene="dapE"
                     /locus_tag="VNY06_06145"
     CDS             3140..4282
                     /gene="dapE"
                     /locus_tag="VNY06_06145"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012386243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009014 - succinyl-diaminopimelate
                     desuccinylase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HWX14421.1"
                     /translation="MTVTALDLCRALVRCPSVTPEDNGALGVLAAALNEAGFGTEKVK
                     FSAPGTPDIDNLYARIGTSAPYLVFAGHTDVVPVGDAARWRFDPFGAEIADGAVWGRG
                     TADMKGGVAAFAAAACAYVARGSPQGSIGFLIAGDEEGPAINGTEKLLRWAYDKGERF
                     DHCIVGEPTSAKMLGDTIKIGRRGSLNATLNALGRQGHAAYPERADNPIPNLMRLLDA
                     LIAPPLDKGTAHFDPSNLEIVSVDTGNPAFNVIPAEVRARINVRFNDLWEPKKLEAEL
                     RQRLDAVAARYALDLEPCNALAFLTEPHSFTKLVADAVEKKTGRRLELSTSGGTSDAR
                     FIRAYCPVLEFGLSGTTAHMVDEHIMVAELEGLTDIYAAILEDYFW"
BASE COUNT          949 a         1276 c         1203 g          908 t
ORIGIN      
        1 gagatcgccc gtagactgaa cgatcatttg caaacggttg ctagtgttac gctgacagag
       61 gtgatgatag ttgaacagat ttctagaccc tgggtcgatc ctgacttctt aggcactctt
      121 aaaatgttag accaattctg cacggattag acgctcggcc cggcgaatga gaggatctgt
      181 acagtgtccg ccttgggcca aacgcggtaa ttcgaactac ctactcacat ccggaatacg
      241 ccgaaacgcg tttcttcgat tgacgcattg agggatgctg aaatgcaaag gccgagtacc
      301 atccgggttt cggccggatc gatgattcca tcgtcccaaa gccgcgcgct cgcataataa
      361 ggacgtgcct ggcgttcaaa acgctcgcga accggcgcct taaaagtctc ctcttcctcg
      421 gcggaccaga gttttgcggc cgcctccaat ttttcccggc gcacttgcgc aagcacattg
      481 gcggcttgct cgccgcccat caccgaaatt cgagcattgg gccacatcca caagaaacgc
      541 ggcccaaagg cgcgcccgca catgccgtaa ttgccagccc caaaactgcc gccaataata
      601 accgtgaatt ttggtacctt cgcgcaagaa accgccgcga cgagcttggc gccgtccttg
      661 gcaatgccct gcgcctcgta ttttggaccg accatgaagc cagtgatgtt ttgcaaaaag
      721 acgagtggaa tttttcgttg cgcacaaagc tcgatgaaat gcgcgccttt aagcgccgat
      781 tcggaaaata aaatcccgtt gttcgcaagc acgcccaccg gataaccaaa aatatgcgca
      841 aagccacaaa cgagtgtcgt gccatagagc gctttgaact cgtcgaacgc gctgccatcg
      901 acgatgcgag cgatgacttc atgcacatca taggccgcgc gcgaatcagc aggaacgatg
      961 ccataaatct cttgcgcatc ataaagcggc tcgaccggct cacggaccac gacgccagga
     1021 ctcttcgctc gatttagatt ggtgacgata tttcgcgcga ggacaagcgc gtgcgcgtca
     1081 ttcgaagcat agtgatcggt cacgccagat ttgcgcgcat ggacatctgc ccctccaagt
     1141 tcttccgccg agacgatttc gcccgtcgcc gctttcacaa gcggcgggcc agctaaaaaa
     1201 atcgtgcctt ggcccgcgac gataattgcc tcgtcgctca tcgcaggcac gtaagcgccg
     1261 cccgcagtgc agctccccat caccacggcg atctgcggaa tgcccaaagc cgacatgcgc
     1321 gcctgattat aaaaaatgcg gccgaaatgg tcgcgatccg gaaagacttc gtcttggctc
     1381 ggaagatgcg cgccgccgct atcgacaaga tagatgcatg gtagattgtt ctcttgcgcg
     1441 atctcttggg cgcgcagatg ttttttgacc gtcagcggat aataagtgcc gcccttgacc
     1501 gttgcgtcat tggcaagcac aacgcaatcc tggcccgata cgcgcccaat gcccgtgatc
     1561 aggcccgccg ccggaacaga gtcgtcgtaa acgttaaaag ccgcgaattg cgacaattcc
     1621 aaaaatggcg tgccaggatc aatgagcgcc gcaatacgct cgcgcggcaa catttttccg
     1681 cgtgcgatgt gccgctcgcg tgcctcaacg ccgccgccct gcgcgatgca cgcggcctta
     1741 aagcgcaagt cctcgacaag agcctccatc gcgactttgt tttcggcgaa gtctttgcta
     1801 tccggcgtga tggcggtctt gaggacattc attgcgctaa tgaccgtcaa acagctcacg
     1861 tccgatcagc atgcgtctta tctcgcttgt gcccgcgcca atctcataga gttttgcgtc
     1921 acgcagcagc ctgcctgtcg cataatcgtt gatatagccg ttgccgccaa gtagctggat
     1981 cgcgtcgagc gcggctctcg tcgcgtgctc ggccgcaaag agcaaagcgc ccgccgcatc
     2041 cttgcgcgac gtgaagccat tatcacaagc gcgagcaact gcatggacat aagagcggca
     2101 agcagttagg ccgacataca tgtctgcgag cttactctgc acaagctcaa attcgccaat
     2161 cgcatggcca aactgctttc ggtcgcgcac atagggcagg caaatatcga gacaggcctg
     2221 catgataccg agcgggcctc ccgccagcac gacgcgctca taatcaagcc ccgacatcag
     2281 tacctgaaca ccatcgccca ccgcgcctaa cacgttctcc tcaggcacct cgcaattctc
     2341 gaacaccagt tccgacgtcg gcgagccgcg cattccaagc ttgtcgagct tttggccgag
     2401 gcgaaaaccc ctaaaactgc tctcgatcaa gaaagcggtg ataccatgag cgccggcatc
     2461 cggatcggtc tttgcataga cgacgagcat cgccgcatga tgcgcgttgg taatccacat
     2521 ctttgtgccg ttcaagatgt agcggccgcc cttcttattc gcgcgcaggc gcatcgatac
     2581 gacatcggag ccggatccca cttcggacat ggcgagcgca ccgacatgct cgccggacaa
     2641 aagctttggg agatagcggc gcttttgctc ctccgtgccg tttcgtcgga tctgattgac
     2701 gcaaagattg gaatgcgcgc cataggaaag gccgacggag gcagaggccc ggcttatttc
     2761 ttccatggcg atgcaatgtg cgagatagcc aaggcccgcg ccgccaaact cttcttcgac
     2821 cgttatgcca tggagcccga gtgcgcccat ctgcggccaa agatcaatcg gaaaagcatc
     2881 gctccgatcg atttcgcccg cgcgcggcgc gatcttggct gaagcaaagg cgtacacagt
     2941 gtcgcggagc ctctccagct cagggccaag ggggaagtcg cgaccgggat tttgcatggg
     3001 tgtagtcctg gaccatccaa aagaactttc ctaaagcata tttggcggca aatttttgca
     3061 cgcaaacagc gttaagcttt taaatgcgaa ggccgccata cgacaaatca cgggcggcgg
     3121 ctgaggaaat cccaaaacca tgacagttac agcactcgac ctttgccgcg cgcttgtgcg
     3181 ctgcccctcg gtgacgccgg aagataatgg tgcactcggc gttcttgcgg cggcactgaa
     3241 cgaggcgggc tttgggacgg agaaagtaaa attttcggcg cctggtacgc ccgatatcga
     3301 caatctctac gcccgtatag gcacaagcgc accctacctc gtctttgctg gccacaccga
     3361 cgtggtgccc gtgggcgacg ctgcgcgctg gcgtttcgac cccttcggtg cagagatcgc
     3421 ggatggcgcg gtctgggggc gcggcaccgc cgacatgaaa gggggcgttg ccgctttcgc
     3481 cgccgccgcc tgcgcttacg tggcgcgcgg atcgccccaa ggttcgatcg gttttcttat
     3541 tgccggcgat gaggaaggtc cggccatcaa tggcacggaa aagctcttgc gctgggctta
     3601 tgataagggc gagcgtttcg atcattgcat tgttggcgag ccgacgagcg ccaaaatgct
     3661 gggcgacacg atcaagattg gaaggcgcgg ctcgctcaat gccacgctca atgcccttgg
     3721 ccgccagggc catgccgcct atcctgagcg ggcggataat cccattccaa acctcatgcg
     3781 gctgcttgac gccttgatcg ctccgccgct cgacaaaggt acggcgcatt ttgatccttc
     3841 caatctcgaa atcgtttcgg tcgataccgg caatccggcg tttaacgtga tccctgcgga
     3901 agtgcgggcg cgaataaatg tccggttcaa tgatctttgg gagcccaaaa agcttgaggc
     3961 ggagctgcgg caaaggctcg atgcggtcgc tgcgagatac gcgctcgatc tggagccttg
     4021 caatgctctg gcgtttttga cagagcccca ttcgtttacc aagctcgtcg ccgatgcggt
     4081 cgagaagaag accgggcgca ggcttgagct gtcaacatcg ggcggcacgt cggatgcacg
     4141 ttttattcgc gcctattgcc cggtgcttga gttcggactt tcggggacga ccgcgcatat
     4201 ggtagatgag catatcatgg ttgcggagct tgagggtttg accgatattt atgcggcgat
     4261 tttggaggat tatttttggt gattatcccc tcgacacacg tggttctata agcgccatgt
     4321 gtatttgttg tggaaa
//
LOCUS       DATMPU010000268         3266 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_1754263,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000268 DATMPU010000000
VERSION     DATMPU010000268.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 3266)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3266)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3266
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1754263"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..292)
                     /locus_tag="VNY06_06150"
     CDS             complement(<1..292)
                     /locus_tag="VNY06_06150"
                     /inference="COORDINATES: protein motif:HMM:NF021603.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2336 domain-containing protein"
                     /protein_id="HWX14422.1"
                     /translation="MVHALANDSSEVASIVLARSPLLSEAELVDCVATADSFAQTAIA
                     LRPWVSSQVCAALAEVGALEALLALASNPGADLLEFSTGRIIERHGNDEDLRA"
     gene            complement(376..513)
                     /locus_tag="VNY06_06155"
     CDS             complement(376..513)
                     /locus_tag="VNY06_06155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX14423.1"
                     /translation="MAIQQFLTWLPAASPAVLAEGAGKLVRAYLYGDLSEPEREDARP
                     P"
     gene            626..1120
                     /locus_tag="VNY06_06160"
     CDS             626..1120
                     /locus_tag="VNY06_06160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009764269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HWX14424.1"
                     /translation="MSDQSQQNPNRHKRGGLFYEDFVQGAVTEHRLTKTVTQMDNMLF
                     SNMTLNPQPLHIDAHFCAQETEWGRPLVNSLFTLGLMIGISVNDLTLGTTIANLGMTE
                     VKFLAPLFEGDTVHVTTEVLGKRSSSSRPDAGIVEFLHRAYQQEGKLVAECRRQAFMK
                     RRAV"
     gene            1140..2012
                     /locus_tag="VNY06_06165"
     CDS             1140..2012
                     /locus_tag="VNY06_06165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015927049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA ester lyase"
                     /protein_id="HWX14425.1"
                     /translation="MRSLLFVPADSERKLEKAIASAADCIILDLEDSVLADAKLKARE
                     RAAAFLAGVTPHSPRPGLYVRVNGLNSGLTSADLDAVMPSRPDGILLPKCLNRASIQH
                     LGAMLAVKEAEHGLADGATRIMAIVTETAASIFNMGTYARASARLECLTWGAEDLAAC
                     LGAESNRGADGLYTFPYVLARSLTLFAAAAAGALAIDAVHGNFRDLAALRCECESAKR
                     DGFAGKMAIHPAQIAIINEIFTPSQESIAKARAIVAAFEAEPQAGVVGFEGEMLDLPH
                     LEKARRLLANIRET"
     gene            2427..2690
                     /locus_tag="VNY06_06170"
     CDS             2427..2690
                     /locus_tag="VNY06_06170"
                     /inference="COORDINATES: protein motif:HMM:NF020250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAF domain-containing protein"
                     /protein_id="HWX14426.1"
                     /translation="MKPARILVLAISLVAGLAAALLASTSKPPEAVVQQPAPPPPPIP
                     TDSVLVAANDLYRGDVLTESKMRWVEWPAEKIPAALRLEPSRS"
     gene            2732..3244
                     /gene="cpaB"
                     /locus_tag="VNY06_06175"
     CDS             2732..3244
                     /gene="cpaB"
                     /locus_tag="VNY06_06175"
                     /inference="COORDINATES: protein motif:HMM:TIGR03177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly protein CpaB"
                     /protein_id="HWX14427.1"
                     /translation="MRRERVALGPHSGFIASDLPPGKRAVAINIDTQGSSTAGGFILP
                     DDTVDVIHIFQDAHFARTVAGNALVSQTILSNVRVLAIGQNVQVNNGERVVTGSNATL
                     EVTPEQAETILLAQRTGSLTLTLRSMSDAKMASEKNKHLAGGLMVVRGGVAKQNKSLS
                     EKLIINYKSF"
BASE COUNT          736 a          941 c          927 g          662 t
ORIGIN      
        1 ccgcgcgcag atcctcgtca ttgccatggc gctcaattat ccggccggtc gaaaattcca
       61 ggaggtccgc accaggattg gaagcaagcg cgagcagagc ctccaaggcc ccgacttcag
      121 ccagcgcggc gcaaacctgt gaggaaaccc acggcctcag cgcgatggcg gtctgcgcaa
      181 aactatctgc ggtggcaacg caatcaacaa gttcggcctc agaaagcaga ggcgaccgcg
      241 caagaacgat tgatgcgacc tcagagctgt cattggcaag cgcgtgcacc atgtaatggg
      301 gcgcattctc ggcacatgcg atgttctcgg caagggcgcg gcgaacagcc ggcgaggggt
      361 gatcgacgag cagtgtcagg gcggccgcgc gtcctcccgc tccggctccg aaaggtcacc
      421 ataaagatag gcacgcacaa gcttgccggc cccctctgcc aacactgcgg gggacgcagc
      481 cggcagccaa gttaagaatt gttgaatggc cattttccgc gaatccagaa aaattgtttc
      541 aaaattgagt ttcctccaac atggtaaagg gagacttaac tctgctggct atggttcggc
      601 catggcggca tgattggaaa atcctatgag cgatcaatcg cagcaaaatc caaaccggca
      661 caaacgcggc ggattgttct acgaagattt cgtccaaggc gcggtcaccg aacaccgatt
      721 gacaaagact gtcactcaaa tggacaatat gttgttttcc aatatgactc ttaatccgca
      781 gccgctgcat atcgacgccc atttctgtgc gcaggagacg gaatggggcc gcccattggt
      841 gaactcgctc ttcacgctcg gtctcatgat cggaatttca gtcaacgatc tcacgctcgg
      901 cacgacaatt gccaatcttg ggatgaccga ggtgaagttt ttggcgcctc tgttcgaagg
      961 ggacacagtg catgtcacga cggaggtttt gggaaagcgc tcctcaagct cccggccgga
     1021 cgccgggatt gtcgaattcc tccatcgcgc ctaccagcag gaaggaaagc tcgttgccga
     1081 atgccgccgt caggcgttca tgaaacgacg ggctgtttga accggcgggg tgtgccacaa
     1141 tgcgttcgct gcttttcgtt ccagccgaca gcgaaaggaa actcgagaaa gccatagcga
     1201 gtgcggccga ttgcatcatt ttggatcttg aagattccgt tttagcagac gcgaaactca
     1261 aggcgcggga gcgggcggcg gcctttctcg ccggcgtgac gccgcacagc ccccgcccgg
     1321 gtctttatgt gcgcgtcaat gggctgaact cgggcctgac aagtgccgat ctcgacgccg
     1381 taatgccgtc gcggccagac gggattctct tgccaaaatg cctcaacagg gcgtcgatcc
     1441 agcatcttgg cgcgatgctc gcggtcaagg aagcggaaca tggccttgcc gatggcgcga
     1501 cgcgaatcat ggcgatcgtc accgagacag cggcctcaat cttcaacatg gggacttacg
     1561 cacgcgcaag cgcccgtctt gaatgcctca cctggggggc ggaagatctt gccgcctgcc
     1621 tcggcgcaga gtcgaacaga ggggctgacg ggctttatac ttttccctac gtccttgcgc
     1681 gaagcttgac gcttttcgct gctgccgccg ccggcgcgct ggcgatcgat gccgtgcacg
     1741 gaaattttcg tgatttggcg gctttgcgct gtgaatgcga atcggccaag cgtgacggtt
     1801 ttgctgggaa aatggcgatt cacccggcac agatcgcgat catcaatgag attttcacgc
     1861 cttcgcaaga atcgattgct aaggcgcgcg caatcgttgc cgccttcgaa gccgaaccgc
     1921 aggcgggagt cgtcggcttc gaaggcgaga tgctcgatct gcctcatctt gaaaaagcac
     1981 gccggttgct ggcaaacatt cgtgaaacgt aacacgcaag agcgcggcgg gcaggcttgg
     2041 ccgcgatgcc aacaatgagg cgcggccgcc gtggcttgcc ccaaccagcc tgcgcccgcg
     2101 gacttctggg ctccttcccg ctaccgcggc gctcatctgc taggttcaac cggctagacg
     2161 gtgtcctctc ccatttggag ttcgcgtatc tattacaaaa ataactgcaa tccttgcagt
     2221 tcgatgaaga tcatacctac cccaatattc gggaccgaaa attaattcga tggcagcgta
     2281 tcactctatc aaattgccat cctccaatta atccaaaatt gccgctcgaa tggtgcaatc
     2341 ggcttctcgc tttgcgcggc caagcctccg tggtcagccg tctaggcgta gaaatgcaca
     2401 cacagacatg acaggcaaaa gtaaaaatga aaccagctcg tattttggtt cttgctattt
     2461 cgcttgtggc gggattagcc gccgcgttgc ttgctagcac ttcgaaaccg cccgaggccg
     2521 tggtgcagca acctgctccg ccgccgcctc ccattccaac cgatagcgtc cttgtcgcgg
     2581 caaatgattt gtaccgaggc gacgtcctca ccgaaagcaa aatgcgctgg gttgaatggc
     2641 cagcagaaaa aattcccgca gcgcttcgcc tggagccatc gaggagctga agggcgcgaa
     2701 gatccgagcg aatctcgctg cgagcgagcc cttgcggcgg gagcgcgtgg ctttgggccc
     2761 gcattctggc tttatcgctt cagacttacc gcctgggaaa cgcgcggtcg caatcaacat
     2821 cgacacgcaa ggttcaagca cggctggcgg gtttattctg cccgacgaca cagtcgacgt
     2881 gatccacata tttcaggatg cacactttgc gcgtacggtc gccggcaacg ctttagtcag
     2941 ccagaccatc ctgtccaatg ttcgcgtgct cgcgatcggc caaaatgtcc aagtgaataa
     3001 tggggaacgc gtcgtcacag gatctaatgc aaccttggaa gtcacgcctg agcaagccga
     3061 aaccatactg ctcgcccagc gcaccggctc gcttacattg accctgcgca gcatgagcga
     3121 cgcgaaaatg gccagcgaga aaaataaaca cctcgccgga ggtttgatgg tggtccgagg
     3181 cggggtcgcg aagcaaaata agagtctgtc cgagaagttg attattaatt acaaatcttt
     3241 ctgagcataa ttcggattga cgcgag
//
LOCUS       DATMPU010000269         3649 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_196548,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000269 DATMPU010000000
VERSION     DATMPU010000269.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 3649)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3649)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3649
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_196548"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            137..556
                     /gene="rplQ"
                     /locus_tag="VNY06_06180"
     CDS             137..556
                     /gene="rplQ"
                     /locus_tag="VNY06_06180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012591972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HWX14428.1"
                     /translation="MYHGHAKRRFGRTHEHRKAMFSNLCQALIKYEQIVTTLPKAKDL
                     RPVVEKLVTLGKRGDLHARRQAIAQIRDTALVNKLFAVLGPRYKDRHGGYTRVLKAGF
                     RYGDNAAMGVIEFVDRDVEAKGMDSGPVSTGEEEAES"
     gene            637..2097
                     /locus_tag="VNY06_06185"
     CDS             637..2097
                     /locus_tag="VNY06_06185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015666154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegQ family serine endoprotease"
                     /protein_id="HWX14429.1"
                     /translation="MKTADPLMGERFTSMRLLGLLRRLPALLLGFFLLSPFAGAETLR
                     QPPASQGETLLSFAPVVKKAQPAVVNVYASRTEKRLPNPFFEDPIFRRFFGDDGSGRP
                     GGPTAHSLGSGVLVDSSGLVVTNYHVIQGMTDVKVALTDKREFDADIILRDQRTDLVV
                     LKLTGGESFPAMDLGDSDALEVGDFVLAIGNPFAVGQTVTQGIVSALARTQVGISDYG
                     FFIQTDAAINPGNSGGALIDMRARLVGINSAIFSQTGSSIGIGFAIPVNMVKIVIAAA
                     KGGGHAVRRPWLGASLQTVSKEIADSLGLERPTGALIADLSAGGPAAEAGLRRGDLIT
                     GIDGQIVEDPEGLGYRLGTKPVGGIASVTLLRDGKQSTAAIMLIPAPEFPARDPIKLT
                     GYSPFSGMTVVNLSPAVLEELSIRGVSEGVAVNEIEEGSRAAMVNFQKGDVILSINDN
                     AIGTTRDLEKAARLRHDFWKLTIERSGEVITTMIGG"
     gene            complement(2104..2745)
                     /locus_tag="VNY06_06190"
     CDS             complement(2104..2745)
                     /locus_tag="VNY06_06190"
                     /inference="COORDINATES: protein motif:HMM:NF013793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HWX14430.1"
                     /translation="MRTIVFATHKGGCGKTTLATCLAVAAQEAGESVIIFDLDPKSCA
                     IRWGSKRMDCNLPVRTLAFARLSSALTAAAKRKVTLVVIDTPVLESPAALAAVKTADL
                     TIVPTRPSAFDIWVAEVTGRRMSFMNERFAFLLNQCPPQRRSRNMRDSIASLKAIGPL
                     LEPQIGVSRRFLKAAATGKGVTEIDPTGEAAHAEALARHQTRAILNTRPQISR"
     gene            2965..>3649
                     /locus_tag="VNY06_06195"
     CDS             2965..>3649
                     /locus_tag="VNY06_06195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016918499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HWX14431.1"
                     /translation="MTDLFAVANLDKGAPHPLADRLRPQSLADVAGQEHLLGPEGALT
                     RLIGSRTLGSLIFWGPPGTGKTTVARLLARETQLAFVQISAIFTGVAELKKVFEEARG
                     RRKTGQGTLLFVDEVHRFNRAEQDSFLPVMEDGTITLVGATTENPSFELNASLLSRAR
                     VMIFQPLTAAALEKLLARAEGCEGHTLPLDAGARLSLVRMADGDGRAALSLAEEAWRA
                     ARPGEILDTA"
BASE COUNT          723 a          993 c         1124 g          809 t
ORIGIN      
        1 ccgaaaacat tgacgaactc gccaagcggt ttgaggagca ttactgatct gatttgcctt
       61 gtctcgtgca aaggaatgag atctaacgag agcggccgta ccttggcacg gcggccgcgg
      121 gataatggag agcgccatgt atcacggaca cgccaagcgc cgcttcggcc gcacgcatga
      181 gcatcgcaag gcgatgtttt caaatttgtg ccaagcgctg atcaagtatg agcagatcgt
      241 gaccaccttg ccaaaggcaa aggatctccg gccggtcgtc gaaaaactcg tgacgcttgg
      301 aaagcgtggc gatctgcatg cgcggcggca ggccattgcg cagatcagag atacggctct
      361 tgtgaacaaa ctctttgccg tgcttggccc gcgctacaag gatcggcacg gtggctacac
      421 gagggttttg aaggcaggct tccgctatgg cgacaatgct gccatggggg tgatcgaatt
      481 tgttgaccgc gatgtcgaag ccaaggggat ggattcgggt ccggtatcaa cgggcgagga
      541 agaggcggag tcctagctcg cgcctgccgg agggcgcccc ccgattggcg cacctctttg
      601 gggtccaaag gggcgcggag gcttggccgt aagtccatga aaacagcaga tccgctgatg
      661 ggggagcgat ttacgagcat gcgcttgctt ggtttgctgc ggcgcttgcc tgctttgctt
      721 ttgggatttt tccttctctc tcctttcgcg ggcgcagaga ctttgcgcca gccgcccgct
      781 tcgcaaggcg agacgttgtt gtccttcgcg ccggtcgtca aaaaagcgca gccggccgtt
      841 gtcaatgttt atgcctcccg caccgaaaag cggctcccaa atcctttctt cgaggatccg
      901 atttttcgcc gcttttttgg cgatgacggc agtggccggc caggagggcc gacggcgcac
      961 tctctgggtt ccggcgttct cgtcgattca tctggtctcg tcgtcaccaa ttatcacgtt
     1021 attcaaggca tgacggatgt gaaagtcgcg cttactgata agcgcgaatt cgacgccgat
     1081 attatcttgc gcgaccaacg cacggatctc gtggtgttga agctgacggg aggcgagagc
     1141 tttccggcta tggaccttgg agattccgac gcgcttgaag ttggcgactt tgtgctcgcc
     1201 atcggaaatc catttgcagt tggccaaacg gtaacccaag gaatcgtctc ggcactcgcc
     1261 cgcacgcagg tgggaatctc cgattacggg tttttcatcc aaaccgatgc cgcgatcaat
     1321 ccgggcaact cgggcggcgc cctgatcgat atgcgggcaa ggctcgtcgg cattaattcg
     1381 gcgatcttct cccagaccgg aagttcgatt gggattggct tcgcgatccc tgtcaatatg
     1441 gtgaaaatcg tgatcgccgc tgccaaggga ggcggtcacg cggtgcgacg cccctggctg
     1501 ggcgcgagcc tgcagaccgt gtccaaggag atcgccgatt cacttggact cgagcggcct
     1561 acgggcgcgc ttattgccga tctctccgcg ggcggtccag cggccgaagc aggattgcgt
     1621 cggggcgacc tcatcaccgg catagatggt cagatcgtcg aagaccccga gggccttggc
     1681 tatcggttgg gaacaaagcc ggttggcggc atcgcgtctg tgaccttgct acgcgacggg
     1741 aagcagtcca ctgctgccat catgctaatt ccggccccgg aatttcccgc ccgggatccc
     1801 atcaaactca ccggttattc gccattctcg ggcatgactg tggtcaatct gtcaccggcc
     1861 gtattagagg aattgtcaat ccggggcgta agtgagggtg tggcggtcaa tgaaatcgaa
     1921 gagggctcga gggcagcgat ggtcaatttt cagaaaggtg atgtgatcct gtccatcaac
     1981 gataacgcta tcgggacgac gcgcgatctc gaaaaggcgg cgcgactgcg gcacgatttt
     2041 tggaagctga caattgaacg cagcggtgaa gtgatcacca cgatgatcgg cgggtgagcc
     2101 aatctagcgg cttatctgag gcctggtatt gaggatggca cgcgtttgat ggcgagccag
     2161 agcttccgca tgcgccgcct cgcctgtggg atcgatctcg gtcacccctt tgccggtggc
     2221 cgccgctttc aaaaatcgtc ggcttacgcc aatttggggc tctaggaggg ggcctatggc
     2281 tttgagcgag gcgatgctgt ctcgcatgtt ccgggagcga cgttgaggcg gacattgatt
     2341 gaggagaaat gcaaatcttt cattcatgaa actcattctg cggccagtca cctcggctac
     2401 ccaaatgtcg aatgcggatg gccttgttgg aacgattgtg aggtcagcgg tcttcacagc
     2461 ggcaagcgcc gcgggagatt ccagcactgg ggtgtcaata acaaccagcg tcactttccg
     2521 cttcgccgcg gcggtcaacg ccgacgaaag gcgcgcaaat gccagcgtgc gcaccggcaa
     2581 attgcagtcc atgcgtttgg agccccatcg aatggcgcag cttttgggat ccaagtcgaa
     2641 aataatgacg ctttcgcccg cttcttgggc cgcgacagca aggcaagtgg caagcgtagt
     2701 tttgccgcat ccgcccttgt gcgttgcgaa tactatggtg cgcattgaaa aaattttatc
     2761 tataaatgaa atgtttaaga acgactcatt tgattagtat tttattgtat ctgaatatat
     2821 gcatgacccg tctgagtttg gaagacgatt ttgccttgct tgcggcgtga gagtgtcttt
     2881 taccttaacg ccgtttcgtc tattggccct gctgttgaac attttctgcc cgctttgcgc
     2941 aatgggaaca atggacaaga ccaaatgacc gacctctttg ccgtcgcaaa tctcgacaag
     3001 ggcgcgccac atccgcttgc ggaccggctg cggccgcaaa gcctcgccga tgttgccgga
     3061 caggagcatc ttttggggcc cgagggcgcg cttacccggc tcattggatc tcgcaccttg
     3121 ggtagcctga ttttttgggg ccctcctggc actggcaaaa cgactgtcgc acggcttctc
     3181 gcgcgcgaga cgcaactcgc ctttgtgcag atttcggcga tttttacggg tgttgccgaa
     3241 ctcaagaagg tgtttgagga ggcgcgaggc agacgcaaga cgggccaggg aaccttgtta
     3301 tttgtcgatg aagtgcaccg cttcaaccgg gctgagcagg acagtttctt gccggtgatg
     3361 gaagatggaa ccatcacgct cgttggcgcc acgaccgaga atccaagctt tgagctcaac
     3421 gcgtcgctgt tgtcgcgcgc gagagtgatg atattccagc cgctcaccgc ggcggcgctc
     3481 gagaagcttc tcgcccgcgc ggagggctgc gaggggcaca ccctgccgct tgacgccggt
     3541 gcccgcttga gcctggtccg gatggcggat ggcgatggcc gcgcggcttt gagccttgcc
     3601 gaggaggcgt ggcgcgccgc cagaccgggg gaaattcttg atacggccg
//
LOCUS       DATMPU010000270         3639 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_2684269,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000270 DATMPU010000000
VERSION     DATMPU010000270.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 3639)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3639)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3639
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2684269"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(570..770)
                     /locus_tag="VNY06_06200"
     CDS             complement(570..770)
                     /locus_tag="VNY06_06200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008970511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2905 domain-containing protein"
                     /protein_id="HWX14432.1"
                     /translation="MSRALILLGLILVVAGLLWPWIGWLQLGRLPGDIVIERPNFTLY
                     FPMTTGLLISLALTLFFWVINR"
     gene            complement(846..1505)
                     /gene="maiA"
                     /locus_tag="VNY06_06205"
     CDS             complement(846..1505)
                     /gene="maiA"
                     /locus_tag="VNY06_06205"
                     /EC_number="5.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011509474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016034 - maleylacetoacetate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylacetoacetate isomerase"
                     /protein_id="HWX14433.1"
                     /translation="MQDPLPNESSLFGYWRSSAAYRVRIALNLKRVTARQIPVQLQRG
                     EQRSENYRAQNPAGLVPLWREPDGFTLAQSLAIIAYLDEIFPDPPLLPADSRQRALCR
                     EIAYTIACDIHPIGNLRVFEKLAADFGARPETPATWNGHWIAAGFDAIEARLNSTAGR
                     HSVGDTISLADICLVPQVYNARRYGVDFTSYPCIVAADTAAKELPAFAMAAPELQPDA
                     P"
     gene            complement(1483..2511)
                     /locus_tag="VNY06_06210"
     CDS             complement(1483..2511)
                     /locus_tag="VNY06_06210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017993694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HWX14434.1"
                     /translation="MKLATLANGTRDGRLVVVSRDLTYATGAHGIASTLQQALDGWPS
                     LAPKLDALAAALELGSVPRERFREHDALSPLPRAFQWIDGSAYLSHVELVRKTRGVPM
                     PPEFLTDPLLYQGGSDSFLAPRQPIVVEDEGFGVDLEGEIGVIVSDVPMGATPAEARA
                     AIALIVLVNDVSLRNLIPAELAKGFGFFQSKPSSAFSPVAVTLDELGAAWDGGCLHLP
                     LLVSLNGKPFGRADGGTGMNFDFPQLIGHAARTRPLSAGTIVGSGTVSNKEGNGGPAR
                     PIADGGIGYSCLVEARMIETIRDGAAKTQFLRFGDTVRIEMKSASGQSIFGAIEQTVE
                     PYARSSAK"
     gene            complement(2533..>3639)
                     /gene="hmgA"
                     /locus_tag="VNY06_06215"
     CDS             complement(2533..>3639)
                     /gene="hmgA"
                     /locus_tag="VNY06_06215"
                     /EC_number="1.13.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018700860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004411 - homogentisate 1,2-dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homogentisate 1,2-dioxygenase"
                     /protein_id="HWX14435.1"
                     /translation="HASRFSRADVPHWKTAPHIARHGLPLGQLRWDPLPIPNEALTFV
                     TGLRTVTTAGDVYAQTGMAAHIFLVTQNMEDDYFLNADGELLIVPQTGGLEIVTELGR
                     MAILPGDIAVIPRGMKFKVLLQDGPSRGYVCENYGARFTLPPRGPIGANCLANPRDFK
                     TPVASFEEKERPCRLMVKWCGAFHLSEIDHSPLDVVAWHGNYAPYKYDMRTFAPVGAI
                     SFDHPDPSIFTVLTAPSGEAGTANVDFVIFPERWQVAEHSFRPPWYHMNIMSEFMGLI
                     FGQYDAKAKGFFPGSISLHNCMLPHGPDKEAFDKASSDELTPQKLENTLAFMFETRYP
                     QQVTAYAASLPTLQTDYADCWQGLGKLFDPKQLK"
BASE COUNT          801 a         1017 c         1066 g          755 t
ORIGIN      
        1 tgcgcagctg cgtcgcatca aaatcggact gaagcggaat tggcatggcg aatctcctcg
       61 ttcgccatgt ggaatcaaat ttgcgccctc gccggaatcc tcttgagtca tgatccacag
      121 gaattggtat tagacgcgag agagagctcg caaacaaggt gagaggcgtt ttgaacagta
      181 ccgccgatga tcggccaaca agaatagcaa aaacgcctgt gtcgctgctt atgcgtgcgg
      241 cgcgcatagc gggatgggca ggaatagcag cgatcacggt cctatctctt gtgccagggt
      301 ccgagcgtcc tcacacaaac cttcccggga tggctgagca tttcgtggct tacgcttgta
      361 ctggctttgc tctgagcctc gcctatcaag gtttgcggga gcgcctgatt ttttgggcag
      421 ccctcgggac agccagcggc gtcttcgaaa tccttcagat gtggataccc ggtcggtcct
      481 gcgaaatcga ggatgtagtc gtcagcacgc tcggcgtaac cactggcctt ctccttggcg
      541 cagctttggc gtcacaacta tcaccagagt taccggttga taacccagaa aaacagcgtc
      601 agcgctaggc tgatgagtag ccccgtggtc attgggaaat agagcgtgaa attcgggcgc
      661 tcgatgacaa tgtcgcccgg caaccggccc agctggagcc atccgatcca cggccacaat
      721 aaaccagcca ctactaaaat cagcccgagc aggatcaatg cccttgacat attgtgttcc
      781 tctccgccgc tctggccccg gctcccatgt ggaaaccacg ggtaatcgcc gggacgaggc
      841 ggtcgctacg gagcgtcggg ctggagttcg ggcgccgcca tcgcaaaggc gggcagctcc
      901 ttcgccgctg tgtcagccgc gacaatacaa ggataagacg tgaaatcgac gccgtaacgc
      961 cgcgcgttgt aaacttgggg gacgaggcag atgtcggcaa ggcttattgt gtcgcccacc
     1021 gaatggcgcc cggcggtaga attgaggcgc gcttcaattg cgtcgaagcc ggcggcgatc
     1081 caatggccgt tccaggttgc gggagtttcg ggccgcgcgc caaaatccgc tgcgagtttt
     1141 tcaaacaccc gcaaattgcc gatggggtgg atgtcgcaag cgatcgtata ggcaatctct
     1201 cggcaaagag cccgctgcct ggaatcggct ggcaacaagg gagggtctgg aaagatttca
     1261 tcgagatagg caatgatggc gagcgattgg gccagggtga acccatccgg ctcgcgccag
     1321 agcggaacaa gacccgcggg attttgcgcg cgatagttct cgctccgttg ctcacctctc
     1381 tgcaattgaa ccggaatttg ccgcgcggtg acacgcttaa gattcaaggc gatccgcact
     1441 cggtaggctg cgctcgacct ccaatagcca aagagggagg actcatttgg cagaggatct
     1501 tgcatatggt tcgaccgtct gttcgatggc accgaaaatg gattggccgc tcgcactctt
     1561 catctcgatt ctcaccgtat cgccaaatcg cagaaattgt gtctttgccg ctccgtcgcg
     1621 aatagtctcg atcattctgg cctcaactag gcatgaatag ccaattccgc catcggcgat
     1681 ggggcgggcg gggccgccat tgccttcctt gtttgagact gtgccagacc cgacgattgt
     1741 gcctgcgctg agtggccttg tccttgctgc gtgaccgata agctgcggaa aatcgaaatt
     1801 catgccggtc ccaccatctg cccgcccaaa aggcttgcca ttgaggctga ctagcagggg
     1861 gaggtgcaag cacccgccgt cccaagcggc gccgagctca tcaagagtga ctgcgaccgg
     1921 cgaaaacgcc gaggacggtt tagactggaa gaatccaaag cctttagcga gctccgccgg
     1981 gatgaggttg cgcaatgaga catcattgac aaggacaata agagcaatag cggcgcgagc
     2041 ctcggccggg gttgcgccca ttgggacgtc gctcacgatg accccgattt cgccctcaag
     2101 atcgacgccg aagccttcgt cctccactac aattggctgc cgcggcgcaa gaaatgaatc
     2161 tgatcctccc tgatagagca agggatcggt caaaaactcc ggcggcattg ggacgcctcg
     2221 ggtctttctg acaagttcga catggctcaa atatgccgag ccgtcgatcc attggaatgc
     2281 gcgtggcaat ggcgagagcg cgtcgtgctc gcggaagcgt tcgcgaggca cggatccaag
     2341 ttcaagcgct gccgcgagtg catcgagctt tggcgcgagg ctgggccaac catcgagcgc
     2401 ttgttggagg gtcgaagcaa tgccgtgcgc gccagtcgca taagtgagat cgcgcgaaac
     2461 caccacaagg cgtccgtcgc gcgtgccatt agcgagtgtc gcaagcttca tgcggtcttc
     2521 ccgctgcgca agtcatttca actgtttagg atcgaaaagc tttcccaagc cctgccagca
     2581 gtcggcgtag tcggtctgca gagttggcag gctggctgca tacgccgtca cctgttgggg
     2641 atagcgggtt tcaaacatga aggcgagcgt gttttcgagt ttttgcggtg ttagctcatc
     2701 ggaacttgct ttgtcgaaag cttccttgtc tggtccatgc ggcagcatgc aattgtgtag
     2761 cgatatactg cccggaaaaa acccttttgc ctttgcatca tactggccaa atatgagccc
     2821 catgaactcg ctcatgatgt tcatgtgata ccagggaggc cggaaagagt gctcggcaac
     2881 ttgccagcgt tccggaaaaa tcacaaaatc gacattggcg gtacccgcct cccctgatgg
     2941 cgcggtcagc accgtgaaga tggaaggatc cggatggtcg aatgaaatgg cgccgaccgg
     3001 cgcaaacgtc cgcatatcat atttgtaagg cgcataattg ccgtgccaag caacgacatc
     3061 cagcggcgaa tggtcaattt cggaaaggtg aaatgcgccg caccacttta ccatgagacg
     3121 gcagggtctt tctttttcct cgaagctcgc tactggagtc ttaaagtcgc gtgggttggc
     3181 gaggcaattg gcgcctattg gcccgcgcgg gggcaaggtg aatctcgcgc catagttctc
     3241 gcaaacatac ccgcgtgagg gaccgtcttg cagcaaaacc ttgaacttca ttccgcgcgg
     3301 aatgacggcg atatcgccgg gtaaaatggc catgcgtcct agctctgtca cgatttccag
     3361 tccgcccgtt tgcggaacaa tcagcaattc gccatccgca ttcaaaaagt aatcatcttc
     3421 catattctgc gttacaagga atatgtgtgc cgccatgccg gtctgggcgt aaacatcacc
     3481 agccgttgtc acagtgcgca agccggtcac gaaagtcagc gcctcgttgg ggataggcag
     3541 cggatcccac cgcagttggc caagcggcaa accatgcctg gcaatgtgag gggcagtctt
     3601 ccaatgcgga acatcggcgc ggctgaagcg actcgcatg
//
LOCUS       DATMPU010000271         3343 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_3309781,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000271 DATMPU010000000
VERSION     DATMPU010000271.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 3343)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3343)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3343
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3309781"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..263
                     /locus_tag="VNY06_06220"
     CDS             <1..263
                     /locus_tag="VNY06_06220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX14436.1"
                     /translation="IMGGGDSVLAELSFTAPAQPVPVDSFVQVVALDTEGSHIAFASY
                     LNRSAILVPLLGGPGRYAWRVYTVATSVPDYMPSAWRYFSVR"
     gene            486..725
                     /gene="rpsU"
                     /locus_tag="VNY06_06225"
     CDS             486..725
                     /gene="rpsU"
                     /locus_tag="VNY06_06225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011474369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HWX14437.1"
                     /translation="MQVLVRDNNVDQALKALKKKMQREGIFREMKLRGHYEKPSEKRA
                     REKAEALRRARKLARKKLQREGLLPMKPKPIFGAR"
     gene            830..1714
                     /locus_tag="VNY06_06230"
     CDS             830..1714
                     /locus_tag="VNY06_06230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012591287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HWX14438.1"
                     /translation="MRGFAALGDEAIFGHPRRKAALSSLLLASALLLAGCETSGILTR
                     PGKGVAEVANQSADEAATNLASLTDVIQRNPASSEAYNTRGVAYARIGKFQEAIADFS
                     QAIKLEPNNAAALTNRALAYRQINRDDVALPDFNRAIEANPKHAPAYLGRANLLRAEG
                     KLDDARADLDQVIKLNSGSAQAYHARGLIYQREGLHEQAITDFDNAIDRDPFAGAPYQ
                     ARGESLIAIGKSDKAIADFDAALNVDSKNAEAWAGLGLAYEKSGNRAKAIESYQHALG
                     FDPNSPIAREGLARNGRG"
     gene            complement(1994..2785)
                     /locus_tag="VNY06_06235"
     CDS             complement(1994..2785)
                     /locus_tag="VNY06_06235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011995521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HWX14439.1"
                     /translation="MKGTVRVGIGGWTFAPWRKSFYPENLSQKRELEYASRQLTSIEI
                     NGTYYRTQKPESFARWYEETPENFVFALKGPMFATSRRNLGEAEASIERFFESGVINL
                     KEKLGPVNWQFPPTKRFDVEDFEAFLKLLPKTVEGHEVRHALEVRHDSFRTAEFVEVL
                     RGYQAGAVFTDKDQFPQIPDITSSFVYARLQRSSIEEETGYPADALDVWAQRALTWSQ
                     GKIPKGLDAVASPWKKPPRDVFIYFINGFKPKAPAAATGLIKRLK"
     gene            complement(2835..2966)
                     /locus_tag="VNY06_06240"
     CDS             complement(2835..2966)
                     /locus_tag="VNY06_06240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX14440.1"
                     /translation="MSDYCRHCRYNPPLAVGEDACPFTTLYGVFLHVMNHGLSVTRA"
     gene            complement(3123..>3343)
                     /locus_tag="VNY06_06245"
     CDS             complement(3123..>3343)
                     /locus_tag="VNY06_06245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX14441.1"
                     /translation="NLCHLGSSFRKNSNPTLAAGFFYRSREKQYRRMGLLVMISVGIT
                     WIVLTEYGVTLRRLASRSIMPMRITLND"
BASE COUNT          725 a          945 c          936 g          737 t
ORIGIN      
        1 caatcatggg aggcggcgac agcgtcctgg ccgagttgtc cttcaccgcg cccgcacagc
       61 ctgtgccagt ggactctttt gtgcaagttg ttgccctcga cacggaaggc tcccatatcg
      121 ccttcgccag ctatttgaac cgttcggcga tcctcgtacc gctgctaggg ggtccgggcc
      181 gctatgcttg gcgcgtttat acggtggcga cctccgttcc tgactacatg ccaagcgcct
      241 ggaggtattt ctccgtgcgt tgatccgcag actatagaag ctttgcgcct tcttgccgat
      301 ctgcttcttt ggcagcgctc tccttcgata acagccgcag cacccgcggt gctagacaaa
      361 gaacgccttt tctgttatcg gcgttctttg cggtcaggtg cctcgcagcg ccgggtgttg
      421 tttgtcttta cgttcatcag cttcgtctta ccgcgcggta gacggcgtca tctagggatt
      481 tctgcgtgca agttctcgtt cgcgacaata atgttgatca agccctcaag gccctcaaga
      541 aaaagatgca gcgggagggc atatttcgcg agatgaaact gcgcggccac tatgaaaaac
      601 cttcggaaaa gcgcgcccgg gaaaaggccg aggctctgcg ccgcgcccgc aagctcgccc
      661 gcaaaaagct gcagcgcgaa ggactgttgc cgatgaagcc gaagcccatc tttggggccc
      721 gttgaacaga tggaggtccg cgggcgaaag atccgcgcat gcgtttgcct gtgaaagtcc
      781 attaggataa cgccagaagc tggcgggcga ttcggacaag ggtgaccgga tgaggggttt
      841 tgcggccctg ggtgatgagg cgatcttcgg acatccgcgt cggaaggctg cgctatcaag
      901 cttgctgctt gcttctgcgc tgctcctcgc cgggtgcgaa acctccggca ttttgacgag
      961 gccaggcaag ggtgtcgccg aggtcgcaaa tcaatcggcc gacgaggccg ccaccaatct
     1021 cgcctcgcta acagatgtca ttcagcgcaa cccggcaagt tccgaagctt ataacacccg
     1081 cggtgtcgcc tatgcgagga ttggcaaatt ccaagaggcg attgccgatt tcagccaagc
     1141 gatcaagctt gaaccaaata atgcggcggc tctgaccaac cgggcgctcg cctatcgtca
     1201 aatcaaccgc gacgacgtgg cgctcccgga cttcaaccgt gcgatcgagg ccaatccgaa
     1261 acacgcgccc gcctatctcg gccgggccaa tcttttgcgc gccgagggca agctcgacga
     1321 tgcgcgtgct gatcttgacc aagttatcaa gctcaactcc ggtagtgcgc aagcctatca
     1381 tgcacgcggt ttgatctatc agcgtgaggg tctgcatgag caggcgatca ccgatttcga
     1441 caatgcgatt gatcgcgacc cttttgctgg ggcaccttat caggcgcgtg gggaaagcct
     1501 gatcgcaatc gggaaaagcg acaaagcgat cgcggatttc gatgccgcgc tcaatgttga
     1561 cagtaaaaat gccgaagctt gggcgggcct cggccttgct tatgaaaaat ccggcaatcg
     1621 ggccaaggcg atcgaatcct atcagcatgc gctcggcttc gatccaaata gcccgattgc
     1681 cagggaaggt cttgcccgca acgggcgcgg ctgaaggatt ttttggacag cgggccccgc
     1741 gggcaaaacc cacaaaccaa aacgtaattg cgctgctgcg gaccttacca aaatgtttca
     1801 cgtgaaacat tttggtccga tctgggccaa caaccttaca aggccgaaga cagcagcgcc
     1861 ttctttgcgc ttgtaagaac gatcgatttt ttggtgcaat caaaattggg cggcgcctcg
     1921 accaatgatt tttcgatccc ccgcctgaga gcgtcccgat gtaaggttcg agcaattaga
     1981 aatttcgcgg cgcttactta agccttttga tcaaacccgt ggcagcggcg ggcgctttcg
     2041 gcttaaagcc attgatgaag tagatgaaaa cgtctcgcgg tggcttcttc caaggacttg
     2101 cgacggcgtc gagccctttt gggatctttc cttgagacca ggtgagggcc cgttgcgccc
     2161 agacatcgag cgcgtcagct ggatagccag tctcctcctc tattgatgag cgctgcaggc
     2221 gcgcgtacac gaaagacgag gtgatatcgg gaatttgcgg aaactggtcc ttgtctgtga
     2281 acaccgctcc ggcctggtag ccgcgcaaaa cttcgacgaa ctccgccgta cggaaactgt
     2341 cgtgtcgaac ttctagggcg tggcggacct cgtggccctc aaccgtcttc ggcagcagct
     2401 tgaggaaggc ttcgaaatct tcaacatcaa aacgctttgt cggcggaaac tgccaattga
     2461 cgggtccgag cttctctttg agattgatga cgccactttc gaaaaagcgc tcaattgaag
     2521 cctctgcctc gccaagattc cgccggcttg ttgcgaacat cggacccttg agcgcgaaca
     2581 caaagttttc gggcgtttcc tcgtaccatc gcgcaaagct ctcgggcttc tgcgtgcgat
     2641 aataggttcc gttgatctcg atcgaagtca gttgccggct cgcatattcg agctcccgct
     2701 tctgcgacaa attttcggga taaaagcttt tacgccacgg cgcaaaagtc cagccgccga
     2761 tgccaacgcg gacggttcct ttcatcggct cggcactcct tgatctttgc gcgcgaagtt
     2821 tttgtactgg aatttcatgc gcgggttacg ctcaaaccgt gattcatgac gtgcaaaaaa
     2881 accccgtaga gtgtcgtaaa cggacaggcg tcctcgccaa cggcgagcgg cggattgtag
     2941 cggcagtggc ggcaataatc gctcatttta taattttatt aatgtaaccg ccgcccgcga
     3001 cataaggctt cgtgccgacc acgccgccgt ttgcgtattg gctcatgcca agcgcgaaat
     3061 gcgccagcga tgtacgtcat gtggtcgagc gccaagctgc aagcaaagga gttcggggat
     3121 cattaatcgt tcaatgtgat gcgcatcggc atgatcgagc gtgaggccaa tcgcctcagc
     3181 gtcaccccat attctgtcag cacaatccac gtaataccaa cgctgatcat gaccaatagg
     3241 cccattcgac gatactgctt ctcgcgagag cgataaaaaa acccagccgc gagggttggg
     3301 ttcgagtttt tccgaaagga actgcctaga tggcaaaggt tag
//
LOCUS       DATMPU010000272         2891 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_2085617,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000272 DATMPU010000000
VERSION     DATMPU010000272.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 2891)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2891)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2891
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2085617"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..430)
                     /locus_tag="VNY06_06250"
     CDS             complement(<1..430)
                     /locus_tag="VNY06_06250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HWX14442.1"
                     /translation="MVGALLGRGRTDIALALQILMNFGNIGLNYFFVYGLSLGARGSA
                     AGTLAAEVLASIGGLCILSRTYSDLFSIDRKQVFERTKIARMFTINRDIFVRIFAFVF
                     FYAQGARDGDVTLAANAVLHNLILVGAFFLDGFATAAEQLC"
     gene            complement(461..913)
                     /locus_tag="VNY06_06255"
     CDS             complement(461..913)
                     /locus_tag="VNY06_06255"
                     /inference="COORDINATES: protein motif:HMM:NF013703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HWX14443.1"
                     /translation="MAAEPGAPSDTKPLVNPPRVDHARVARLALPMTLAHLSTPLLGG
                     ADAAIIGRLGQAHLLGAIASAAIMFNFLFWSFGFLRMGTAGLTAQALGACDAAEQRAT
                     LLRALMVAFAIGSLMILLQAPIGWICIGALDASPDVSTAARLYFDIRI"
     gene            complement(903..1274)
                     /locus_tag="VNY06_06260"
     CDS             complement(903..1274)
                     /locus_tag="VNY06_06260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012384070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6460 domain-containing protein"
                     /protein_id="HWX14444.1"
                     /translation="MSEEDRIAPNPPPPWPQRPIPEQGRAAAENQPRGETALNRFLGG
                     SPGSVFMRLLFVSLIVGAFLMWLDISPWDVFRGLQQLIDRIWGLGFDAIREIINYVLA
                     GAVLVVPVWLVLRLMNIRNGR"
     gene            complement(1369..2562)
                     /locus_tag="VNY06_06265"
     CDS             complement(1369..2562)
                     /locus_tag="VNY06_06265"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018329293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HWX14445.1"
                     /translation="MGSADPIVIVAAARTPIGGFQGELKDVPASALGAVAAKAAVARS
                     GIAPEAIEEVILGCVLPAGQGQAPARQASLAAGLPEAAPCTTINKMCGSGMKAIMLAH
                     DILSAGTARIALAGGMESMSNAPYLLDRARSGYRMGHGKILDHMFLDGLEDAYDKHLP
                     MGAFAEDCAEKLQFTRSAQDAFAIASLERAQRAISDGSFDKEIEPVRVNGKSGAKIVL
                     HDEQPLKAKRDKIPYLKPVFRQDGTVTAANSSSISDGAAALVLMPRSEADRRAIAPLA
                     EIRAHATHAQAPSAFPTAPIGALRKLMDKTGWSLADVDLFEINEAFAVVTLAAMQELS
                     LPHEKVNLHGGACALGHPIGASGARIVVTLLAALQRYGLKRGAAALCIGGGEATALAL
                     EVPQY"
BASE COUNT          637 a          848 c          858 g          548 t
ORIGIN      
        1 cgcataattg ttcggcggcg gttgcaaaac cgtcgaggaa aaaggcgcca acgagaatga
       61 gattgtgcaa aaccgcgttg gcggcaagcg taacatcgcc gtcgcgcgcg ccctgagcat
      121 agaaaaacac gaaggcgaag atgcggacaa agatgtcgcg gttgattgta aacattcggg
      181 caatctttgt ccgctcgaaa acctgttttc gatcaataga aaaaagatcg ctgtaggtgc
      241 gcgacaggat gcacaaccct ccgatggaag ccagaacctc ggccgcaagc gtacctgccg
      301 cagacccccg cgcaccgagc gacaagccat aaacgaaaaa gtagttgagg ccaatattgc
      361 cgaaattcat gaggatttgg agcgcaagcg cgatatctgt gcgcccgcgc ccgagcagcg
      421 cgccaaccac cgcgtagttc gcaagcgtga agggtgccga ctagattctt atgtcgaaat
      481 agagccgcgc ggcggtgcta acatcgggcg aggcgtcaag agcgccaatg catatccagc
      541 ctattggtgc ctggagcaag atcattaagg acccaattgc aaaagccacc ataagggcgc
      601 ggagcaaggt cgcgcgctgc tcggcggcgt cgcacgcgcc aagcgcctgc gccgtgagac
      661 cagccgttcc catgcgcagg aatccaaaac tccagaataa gaagttgaac atgatggcgg
      721 cggaggcgat cgcgccgagg agatgtgctt gcccgagcct gccaatgatc gccgcgtcgg
      781 cacccccaag cagcggcgtc gacagatgcg caagcgtcat gggcaaagcg agccgcgcga
      841 ccctcgcgtg gtcgacacgc ggcggattca cgaggggctt tgtatcggac ggggcgcccg
      901 gctcagcggc cattgcggat attcatgagc cgcagcacca gccaaacagg aacgacaagc
      961 accgcacccg caaggacata gttaatgatc tcgcgtatcg cgtcaaaccc aagaccccag
     1021 attcgatcaa tgagctgttg gagcccacga aagacatccc aggggctgat gtcgagccac
     1081 atgaggaatg cgccgacaat cagcgataca aagaggaggc gcatgaacac gctcccgggc
     1141 gagccgccca gaaagcggtt tagcgcagtc tccccgcgcg gctgattttc tgctgccgca
     1201 cggccctgct ccgggatcgg gcgctgcggc caagggggag gtggatttgg cgcgatacga
     1261 tcctcttcgc tcatgcgttc tctctcaact ctgcgttcct tttcaacccg aagtcattcg
     1321 tggctgctcc aaacacgaaa tgaccaggca gcgggttgcc gcctcgtgtc aatactgtgg
     1381 cacctcgagc gccagggcag tggcttcgcc accgccgata caaagcgccg ccgcaccacg
     1441 tttcaagcca tatctttgca gcgctgcaag taaggttacg acaattctgg cgccagacgc
     1501 gccgatcgga tggccgagcg cgcaagcgcc gccatgcaaa ttgactttct catgcggcaa
     1561 ggaaagctcc tgcatcgcag ccaaagtcac aaccgcaaag gcttcattga tttcgaagag
     1621 atcgacgtct gccagcgacc aacccgtctt gtccatgagt tttcgcaaag cgccgatcgg
     1681 cgccgtggga aacgcgcttg gtgcctgcgc atgggtggca tgggcgcgga tctccgcgag
     1741 aggcgcgatg gcacgccggt cagcctcgga gcgcggcatg aggacgagcg ccgccgctcc
     1801 atctgagatt gagctggaat ttgccgccgt taccgtgccg tcctgccgga acacgggttt
     1861 cagataaggg attttgtcgc gcttggcttt gagcggctgt tcgtcatgca agacgatctt
     1921 ggccccggac ttgccgttca ctctgacagg ttcaatctcc ttgtcaaaac tgccatcgga
     1981 gattgctctt tgcgcgcgct ccagcgaagc gatggcgaac gcatcttggg ctgaccgggt
     2041 aaattgcaat ttttcggcac agtcttctgc gaaggcgccc ataggcaaat gcttatcata
     2101 ggcatcctcc agcccgtcga ggaacatatg atcgaggatt ttgccatgcc ccatgcgata
     2161 gccggatctc gcgcgatcaa gcaaataggg cgcgttgctc atgctctcca taccgcccgc
     2221 gagcgcgatc cttgcggttc cggcggatag aatgtcatgt gcgagcatga tggctttcat
     2281 gcccgaacca cacattttgt tgatggtggt gcagggcgcg gcctcgggca agcctgcggc
     2341 cagcgaggcc tgccgcgctg gcgcctgccc ctgccctgct ggcaagacgc aaccaagaat
     2401 gacttcttcg atcgcctcgg gcgcgattcc cgagcgcgcg acggccgcct tcgcggcaac
     2461 cgcgccgaga gccgaagcgg gaacgtcctt tagctcgcct tgaaagccac caatcggcgt
     2521 gcgcgcggcg gcaacgatga cgattgggtc ggcactgccc atgatcattc ctcccttaag
     2581 ggttgataaa ttccattcgc ctgagttctg ccaatgagct ccgcccgtgc gcgcaaaatt
     2641 tatatcgcct ggcgctgaag ttgtcttaac attccccgat tagaatgggg gatgccgcta
     2701 gggtttgcgc gagtgacgcc cagcatggct caggagaggc taaacatatt ggtgcggttg
     2761 acacagtgca caacccgcaa gcgagcaagg cgggacctcg acggacggga agcgggatcc
     2821 gcgctagcat ttattttttt gtcgtgcgtt tcttttaagg taaagtctct tcattagagg
     2881 agtatttttt g
//
LOCUS       DATMPU010000273         4224 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_1757270,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000273 DATMPU010000000
VERSION     DATMPU010000273.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 4224)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4224)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4224
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1757270"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            500..946
                     /locus_tag="VNY06_06270"
     CDS             500..946
                     /locus_tag="VNY06_06270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX14446.1"
                     /translation="MGQDQDTNLAGAYLNQFKEIVERLPSAPELVGDLLNWRPTSYND
                     YFSAAAMPGRASAADVYASLNRFSQKSFEGVAGDLDCKALGAAAALRRHYKTHGEARP
                     DIMMKICDRAGKNLREALGKANSLASHEPGTSANVVSGCRFQINQA"
     gene            1159..2481
                     /locus_tag="VNY06_06275"
     CDS             1159..2481
                     /locus_tag="VNY06_06275"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011087644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HWX14447.1"
                     /translation="MRLSQYFLPILREVPKEAEIVSHRLMLRAGMIRQEAVGIYAWLP
                     LGFRVLQKIGQIVREEMDRAGAIELLMPTLQLADLWRQTGRYEAYGPEMLRIADRHKR
                     ELLYGPTNEEMITEIFGAYIRSYRDLPKILYHIQWKFRDELRPRFGVMRGREFLMKDA
                     YSFDVDEASAHISYNKMFVAYLRIFARMGLKAIPMRAETGPIGGNLSHEFLILAETGE
                     SAVYCHAGVLDMPVPAENTDYESDLAPIIGEWTKFYASTGDVHDPTRFEQETPEAKRI
                     HTRGIEVGQVFYFGEKYSKPMRALITHPDGAEQPCQSGSYGVGVSRLAGAIIEASHDD
                     NGIIWPEAVAPFKVGIANLKTGDSAADRASEEIYGALQKSGVEVLYDDTDERPGAKFA
                     RLDLIGLPYQLIVGPKSLAEGLVELKIRATGARKNLDIATAIAQFAGR"
     gene            2481..3746
                     /locus_tag="VNY06_06280"
     CDS             2481..3746
                     /locus_tag="VNY06_06280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018407641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit"
                     /protein_id="HWX14448.1"
                     /translation="MDDAKGKRPFAPFEWMIALRYLRARRRTSFVSVIALFSFLGIML
                     GVATLIVVMSVMNGFHKELLEKIVGINGHIFLQAADTPFADYDEVVRQVAAVPGVELA
                     VPMIESPAAASTSNMAFALVRGIREADIKRLPGIAHNVKLGTLDGFDTAGGVAIGQRL
                     AENLGLRIGDKVKILTANGAQTPFGVTPRSKVYPVTAIFQIGMAEFDNLFVYVSFPEA
                     QGFFNKDSQASVIEIFVSKPEQIDGVKDRIERAAMRPMIMTDWRERNKTFFDALNVER
                     NVMFIILTLIVLVAALNIVSGLIMLVKDKGRDIAILRTVGATQGGVMRIFLITGASIG
                     VAGTIAGCLLGLLLATNVEALRQFLNRLLNANLFPAELYFLSRLPALVDLRDVVTVAA
                     MALTLSVLATLYPSWRAAKLDPVEALRYE"
     gene            3749..>4224
                     /locus_tag="VNY06_06285"
     CDS             3749..>4224
                     /locus_tag="VNY06_06285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020176059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HWX14449.1"
                     /translation="MAPPALHLAKVERRYDQAGAPLVVLRGADFSLWGGQSVALVAPS
                     GAGKSTLLHVAGLLERPDGGEVYIAGRATVAMPDDARTAIRRGEIGFVYQFHHLLPEF
                     TALENVMLPQMIAGLSTREAKERAGQLLRYLGLSEREDHLPAELSGGEQQRVAIARA"
BASE COUNT          867 a         1156 c         1241 g          960 t
ORIGIN      
        1 gagaaaagta ccgttgcggc tgaaggcttg tggtaaggcg gcgcccgcaa tttcttgcgc
       61 ttcgtcagga tagccgagca aaatttcccc tgtcgcgagc ggccgccaat tgccttcgct
      121 atcgagagca ccattgccgt ccctaaaatt ccgctccaag cggtttggat atttcccgcc
      181 gaaaaccgga tcaccgatgc tgtcggtaaa gccaaaatgt tcttttggag agaccgctaa
      241 gcctaaggcg gaggcgatta cgcgcctctt cggggccttg tggcaaggct ctgccttggt
      301 gccaagcctc ggcggcgggg gtcgggcgcg ccggtcgcaa actactgtct ccggaaacca
      361 atttttatcg gttcgcggct agtttgaaat gacgaataga ggcaattgcg aaatgacacc
      421 agctgcatcg acgccaaaga catcgaattc ttcgccgatg acggaaggat ctcgcccagc
      481 accgcgacct tgcggcccaa tgggtcagga tcaggatacc aaccttgcgg gcgcctatct
      541 taatcaattc aaagagatcg tcgagcggct gccgagcgcg ccggaattag ttggtgatct
      601 tttaaactgg cgtccgacga gttacaatga ctacttctct gcggccgcaa tgccgggccg
      661 cgcgtctgcc gcggacgtct atgcctcctt gaaccgattc tcgcaaaaaa gcttcgaagg
      721 cgttgccggg gatcttgact gtaaggcgct cggcgcggct gcggccctaa ggcgccacta
      781 taagacccat ggcgaagcgc gtcccgatat catgatgaaa atttgtgacc gcgcgggaaa
      841 gaatctccgt gaggcgctgg gcaaggcgaa ttctctcgcc agtcatgaac cgggcacgag
      901 cgctaacgtt gtttcggggt gcaggttcca aatcaaccaa gcttgagcgc aagcgatatt
      961 ctgtaggaca gcagaatttt accggcaggc caaggtaact ccgccctaaa tgccggggct
     1021 tactttggcg tggcagccca aatgagctta tggcactttg ggcgacgtta gtttttttgg
     1081 tgcctttgcg tttcccggcg ggtggactaa aacccaaagc ccgcccgttt gattcttctt
     1141 gaaacgcgtt tcctgctcat gcgcctatcc caatattttc tgcctattct ccgcgaggta
     1201 ccgaaagaag ccgaaattgt ctcgcaccgt ttaatgctgc gcgccggcat gattcggcag
     1261 gaggcggtgg gcatttatgc ctggctgccg ctcggctttc gcgttctgca gaaaatcggg
     1321 caaattgtgc gcgaggaaat ggaccgggcg ggcgcgatcg aacttttgat gccaaccctg
     1381 cagcttgccg atctgtggcg gcaaacaggc cgctatgagg cttatggtcc ggagatgctg
     1441 cgaatcgcgg accgtcacaa gcgggaattg ctatacgggc cgaccaatga ggagatgatc
     1501 accgaaattt ttggcgccta tatccgttca taccgggact tgccgaaaat tctttaccat
     1561 atccagtgga aattccgcga cgaattacgg ccgcgctttg gcgtgatgcg cggacgcgag
     1621 tttttgatga aggatgccta ttccttcgat gttgatgaag cttcggcgca catttcctac
     1681 aataaaatgt tcgttgccta tttgcggatt tttgcccgca tgggcctgaa ggcgatcccg
     1741 atgcgggccg aaaccgggcc gatcggtgga aatttgagtc acgagttcct tattctcgca
     1801 gagactggag aatcggcggt atattgccat gcaggtgtcc tggatatgcc agtccctgcg
     1861 gaaaacacgg actatgagag cgatcttgcg cctataattg gcgagtggac aaaattttat
     1921 gcttcgaccg gagacgtcca cgatcccacg cggttcgagc aggagacgcc cgaggcaaag
     1981 cgcatccaca cgcgaggcat tgaagtcgga caagtgtttt atttcggcga gaaatattcc
     2041 aagccaatgc gcgctctcat tacccatcct gacggagccg agcagccgtg ccagagcggc
     2101 tcctatgggg tcggcgtgtc gcgccttgcc ggcgccatca ttgaagctag ccatgacgac
     2161 aatggaatca tttggcccga ggctgttgca ccgttcaagg ttggaatagc caatctcaag
     2221 acaggtgata gtgcagccga cagggcctcc gaagagattt acggtgcgct ccagaaatcg
     2281 ggagtcgaag tgctttatga cgatacggac gagcgtcccg gcgccaaatt cgcgaggctc
     2341 gatttgatcg gcctgcctta tcagctcatc gttggcccca agagcttggc cgaggggctg
     2401 gtggaactca agatcagggc aacgggcgcg cggaaaaatc tcgatatcgc cacggcaatc
     2461 gcgcaatttg cgggccggtg atggacgacg caaagggcaa gcgcccattc gcgcctttcg
     2521 aatggatgat tgctctgcgc tatctgcgcg cgcggcgaag gacgagcttc gtgtcggtca
     2581 tcgcgctttt ctcttttctc ggcattatgc ttggcgttgc aaccctgatt gtcgtcatgt
     2641 cggtgatgaa tggttttcat aaggaattgc tcgaaaagat cgtcggcatc aacggccaca
     2701 ttttcctcca ggccgccgat acgccttttg ccgattatga tgaggtggtg cggcaagtcg
     2761 cggcggtgcc cggtgtcgaa ttggcggtgc cgatgatcga gagcccggcg gcggcgtcaa
     2821 ccagcaatat ggcctttgcg ctggtgcgtg gaattaggga ggccgacatt aagcggcttc
     2881 cgggaattgc tcataacgtc aagcttggca cgctcgatgg ttttgatacg gccggcggcg
     2941 tcgcgatagg ccaaagactt gccgaaaatc tcggcctgcg catcggcgac aaggtcaaaa
     3001 tcctgaccgc aaatggtgcg caaacgccct tcggcgtcac cccacgcagc aaggtctatc
     3061 cggtcaccgc gatctttcag atcggcatgg cggaatttga caatctcttc gtctatgtct
     3121 cctttcccga ggcgcagggc ttcttcaaca aggacagtca ggcaagcgtc atcgaaattt
     3181 ttgtgagcaa acccgagcag atcgacggcg tcaaagacag gatcgagcgg gcggcgatgc
     3241 ggccgatgat catgaccgac tggcgcgagc gcaacaaaac cttttttgat gcactgaatg
     3301 tcgagcgcaa tgtgatgttt attatattaa ctttgatcgt gctcgtcgcc gcgctcaata
     3361 tcgtctcagg gctcatcatg ctggtcaagg acaaagggcg ggacattgcg attttgcgca
     3421 ccgtgggcgc gacgcaaggc ggagtgatgc ggatttttct tatcaccggt gcctcgatcg
     3481 gcgttgctgg gacgattgcc ggatgtctgc tcggccttct cttggcaacc aatgtcgagg
     3541 ctctgcgcca attcttaaac cgcctcctca acgccaatct ctttcctgcc gaactttatt
     3601 ttctctcgcg tttgccggcg ctggtcgatt tgcgggatgt cgtcactgtt gccgctatgg
     3661 cgctcacgct ttccgtgctg gcaacgctct atccgtcgtg gcgcgcggca aagctcgatc
     3721 cggttgaagc gctgcgttac gagtaattat ggctccgcca gcgctccatc tagcaaaggt
     3781 cgaacgccgc tatgatcagg cgggggctcc gctcgtagtt ttgcgcggcg ccgatttttc
     3841 cctttggggc ggacaatcgg tggcgctcgt tgccccttct ggagcaggca aatcgacgct
     3901 cctccatgtc gcgggccttc ttgagcggcc ggacggcggc gaagtttata tcgctggtcg
     3961 agccactgtc gccatgcccg acgatgcgcg cacggcgatt aggcgcggcg agatcggttt
     4021 tgtttaccag ttccatcatt tgctgccgga attcacggcg ctcgaaaatg ttatgttgcc
     4081 gcaaatgatc gccgggcttt ccacgcgaga ggcgaaagag cgcgctggcc aattgctgcg
     4141 ctatctggga ctctccgagc gcgaagatca cttgcccgcg gaactttccg gcggcgaaca
     4201 gcagcgtgtc gcgattgcgc gcgc
//
LOCUS       DATMPU010000274         4498 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_2087042,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000274 DATMPU010000000
VERSION     DATMPU010000274.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 4498)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4498)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4498
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2087042"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..271
                     /locus_tag="VNY06_06290"
     CDS             <1..271
                     /locus_tag="VNY06_06290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012045192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="monooxygenase"
                     /protein_id="HWX14450.1"
                     /translation="AGMAIEDGAVLAKCLAEGHGDSAADVAAALKRYGRLRRSRVAQV
                     QRAARQAGRIYHLTGPAAMARDFTIRAIGPQRMLARQDWIYDWRV"
     gene            complement(422..853)
                     /locus_tag="VNY06_06295"
     CDS             complement(422..853)
                     /locus_tag="VNY06_06295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011503684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX14451.1"
                     /translation="MIDRARRIWPRPGLAMTMALFGLGALSGCADMSDSMTSAFADPA
                     KYDLYDCKQLEPERKTLANRAAELQGLMAKAETGAAGSVVSELAYRNDYIAIRGQQKF
                     AEEAWRRNKCHESPPAKTDTPIAPSANARRAPPSRSGGAVY"
     gene            complement(1225..2511)
                     /locus_tag="VNY06_06300"
     CDS             complement(1225..2511)
                     /locus_tag="VNY06_06300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018266080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HipA family
                     toxin"
                     /protein_id="HWX14452.1"
                     /translation="MTSEKKPTEAFVWVWLPGETVPVVAGRLSGAGDRLLFNYGRSYL
                     ARNNAIALYEPELPLQNGVLPLTPGLKMPNCIRDAAPDAWGRRVIINHKFGKSSKDIV
                     TTELDEFTYLLESGSDRIGALDFQLSADRYEAREASAASLEELQSATALVEKGVPLTP
                     DLEAALLHGSSIGGARPKAMVVSNNKKFVAKFSSQNDLYNVVKAEFVAMWLAEKAGIA
                     TASVRLERAAGKDVILIERFDRQRTGDRWCRRAMVSALTLLGLDEMMARYASYEDLAT
                     VIRHRFVSPKPALKELYARMVFNVLCGNTDDHARNHAAFWNGSELSLTPAYDICPQGR
                     SGGEATQAMLIMGEQRSSEISLCLKTAPLFLLNSDEATQLVGNQIKTIKKFWTEVCDA
                     AALTEVDRNFMWRRQFLNAFAFVDAPDALAELVGQL"
     gene            complement(2501..2836)
                     /locus_tag="VNY06_06305"
     CDS             complement(2501..2836)
                     /locus_tag="VNY06_06305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011654946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HWX14453.1"
                     /translation="MPKKTRPHSRYAKDAAVLLGQLIRKARIDHKMTAEELADRAGLS
                     RGLLRRIENGDLGCTLGAVFEAAAVVGVRLFDTDQAALMSAVASNTAVMTLLPKAVRA
                     SQVGLDDNF"
     gene            complement(3290..3571)
                     /locus_tag="VNY06_06310"
     CDS             complement(3290..3571)
                     /locus_tag="VNY06_06310"
                     /inference="COORDINATES: protein motif:HMM:NF021447.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2158 domain-containing protein"
                     /protein_id="HWX14454.1"
                     /translation="MRSFTKRTLAAIVFGVALCLPSIPAFSDPAPSTSTLQSWTAPSL
                     RRGDLVRLRSGGPLMTVENIKGSEVDCYWSDWNGQPNAQAFPIEVLQKF"
     gene            complement(4123..4350)
                     /locus_tag="VNY06_06315"
     CDS             complement(4123..4350)
                     /locus_tag="VNY06_06315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX14455.1"
                     /translation="MKRLTISALAVAALIVAAIFFPHSRSPSIALSPSVVLSTAAMPP
                     LEDLHAMAAVSKLPIQEMEDQSLVYPTVTKR"
BASE COUNT          965 a         1262 c         1304 g          967 t
ORIGIN      
        1 cgcgggcatg gcgatcgagg acggggcggt gctggccaaa tgcctcgccg aaggtcatgg
       61 cgacagcgcc gctgacgtgg cagccgcgct gaagcgctac gggcggctgc gccggtcgcg
      121 cgtggcgcag gtgcagcgcg cggcgcgaca ggcgggacga atctatcacc tcaccggtcc
      181 agctgcgatg gcgcgcgact tcacgatcag ggcgatcggc ccgcagcgca tgctggcgcg
      241 gcaggactgg atttacgact ggcgggtata ggatattgcg gcgtgtgcgc gatcgaccac
      301 gatctcattt ttttgagcat gatttccgcg caaacgcgct ttcgcgtttg ttagctaagg
      361 ggaaaaccgg ttcccaccca tcgggtcgtg gcccgagcgc aggctttttc cggatcatgc
      421 tctaataaac cgcaccaccg gaccgcgacg gcggcgcacg tcgcgcattc gcggatggcg
      481 cgatgggcgt gtccgtcttg gccggcggag attcatggca cttgttgcgg cgccaggctt
      541 cctcggcgaa cttttgctgg ccccggatgg caatgtagtc gttgcgatag gcgagttcgg
      601 agaccactga acctgcggcg ccggtttcgg ccttcgccat caggccctgc aattccgcgg
      661 cgcgattggc gagggtcttg cgctccggct ccagttgctt gcaatcgtac agatcgtatt
      721 tggcgggatc ggcgaaggcc gaggtcatgc tgtcgctcat gtcggcgcag ccggaaagcg
      781 caccgagtcc gaacagcgcc atcgtcatgg cgaggcccgg ccgaggccaa atgcggcgcg
      841 cgcgatcgat catgcggctt tcgtaatcgg acaacattga gattgcctaa agcaggggga
      901 gatgtccgca atgaggcttt accttggccc tcccgccgcc tcaaggaagg gttgtttttc
      961 ggcctgatac cctcctaatt acacttcacc ggaaaacgcg ctaagtgcct gatctcttaa
     1021 gcaaagcgga cgtggcggaa ttggtcgacg caaggcctca caaatgggaa gaggcaatgg
     1081 acacgctcga tctcaccgcc atggccgtca ttggcttgct tattgctgct aatgagatcg
     1141 tcgaagctgg cgtaaatttt ttgtcccttg ctgatgaata tcaggcgctc caaacggcga
     1201 gtataaagtc aggctttgta aagatcagag ttgaccgacc agttcagcga gcgcatctgg
     1261 tgcatcaacg aacgcgaagg cattcaagaa ttgacgcctc cacatgaaat ttcggtctac
     1321 ttccgtgagc gcagccgcat cgcaaacttc cgtccagaac ttcttgatcg tcttgatctg
     1381 gttgccgacg agctgggtgg cctcgtcact gttgagcaaa aagagcgggg ccgtcttgag
     1441 gcacagcgat atttcgctac tgcgttgttc gcccatgatc agcattgcct gcgttgcttc
     1501 cccgcccgac cttccttgcg gacagatgtc gtaggctggc gtaagcgata gctcgcttcc
     1561 attccagaac gcggcatgat ttcgggcatg atcgtccgta ttgccgcaaa gcacattgaa
     1621 caccatcctt gcgtacagtt cctttagtgc cggtttcggc gacacgaacc tgtgacgtat
     1681 gacagtcgcg aggtcttcgt aactggcata acgggccatc atttcgtcga ggccgagtag
     1741 cgtcagagcc gaaaccatag cgcggcgaca ccaccggtct ccggtccttt gccgatcaaa
     1801 gcgctcgatg agaatgacat cctttccggc cgcgcgttca agcctaacgg aggccgtggc
     1861 aatgcccgct ttctctgcaa gccacatggc tacgaactcg gcctttacga cgttgtataa
     1921 atcgttttgc gatgaaaact tggcgacgaa cttcttgttg ttcgaaacga ccatcgcttt
     1981 cggccgggcg ccgccgatcg aacttccatg aaggagagca gcttcgaggt ctggagtgag
     2041 tggaacccct ttttcgacaa gcgctgtcgc gctttgaagt tcttcgagcg aggctgccga
     2101 agcttccctc gcctcatacc gatctgctga tagctggaaa tccaaagcac cgatgcggtc
     2161 ggaaccagat tccagaaggt aggtgaattc atcaagctcc gtcgtaacaa tatctttgct
     2221 actctttccg aatttgtggt tgatgatgac gcgccgaccc caagcatcag gcgcggcatc
     2281 acgtatgcaa ttcggcattt tcaatccggg cgtaaggggc aagacgccgt tttgcaacgg
     2341 tagctccggt tcatagagag caatcgcgtt gtttcgcgca agataactac gcccgtagtt
     2401 gaagagcagc cgatcccctg caccggagag tctgccagct acaaccggca ccgtctctcc
     2461 aggtaaccag acccaaacaa aggcctcggt gggctttttt tcagaagtta tcatcgaggc
     2521 caacctgtga ggcacggacg gctttcggta acagcgtcat cacggcagta ttagacgcga
     2581 cggcgctcat gagtgctgcc tgatctgtat cgaacaagcg cacgccaaca acggcggcgg
     2641 cttcgaatac ggcgccgagc gtacagccga ggtctccgtt ctcgatgcgt cgcagaagac
     2701 cgcgcgacag gcccgcccgg tcggcgagtt cttcggctgt catcttatgg tcgatacgcg
     2761 ccttacggat gagctgcccc aggaggacag ccgcatcctt agcgtatcgg gagtgaggtc
     2821 ttgttttctt tggcattttt gatccattaa tagaccatgt gatctcttat ggactattta
     2881 tagatcaaat cctggcgttc gtcaagcaat gagctgttaa tgatccatat gaggtcaaga
     2941 ggtctattaa tagatcattt ttgttgtgag gtcgggttcc caaaacagct tcccagaaag
     3001 tagcggagcc tcataaattg gctgctaggt cgctgaaatc ggctgaatcc cctctccttt
     3061 cgacgatggg tgttagtcct tgattgaaca tgtctatcga tttccccctc tcaggaattc
     3121 acgcgtagca gcgcgggcgt aatcaactca cgcgcctgtc aatgatagtt caggttgtcg
     3181 gacattcact cgtcgcttga ggtcgccatg catgggaaat ggtcgcgatg taaaccgtac
     3241 acgctcaaca ccgcgcgatc agtgactggt tcgggctgag agacggcgat cagaacttct
     3301 gaagcacctc gatagggaaa gcttgcgcgt taggctggcc attccagtcg gaccaataac
     3361 aatccacctc actgcccttg atgttctcaa cggtcatcaa tggccctccc gaacgtagcc
     3421 gaacaagatc accacgccgt aatgaaggag cggtccagct ctgcaatgtg gaagtcgatg
     3481 gagcggggtc ggaaaaagcc ggaatcgaag gtagacaaag cgcgaccccg aaaacaattg
     3541 cggccagagt gcgtttggtg aacgagcgca tgttaaactc cacttccagt ggagctgaca
     3601 tagggacgca tttcgctgaa accactaaat tgcattttaa aagtggtatg tccaagggac
     3661 ggacgatgca tgcctacgcc gcgatggaac aaagtccgaa cccaaaggcc gttggtatac
     3721 gaaagccgcg gcgaagccgt cggcgcgtca gatagcggac atcgccgcca tccgcatcgc
     3781 cgaccgatcg gacggccgtt ttggcagtcc gatcctgagc cacgggcacg tcggatgtca
     3841 ggggcaatga tgctcttgag tggcgccacc tgaggtggcc gtctaaaaca gaatttaatg
     3901 gggatcagcg gctggcgaac gcacatcaga atcgtgccgg cctctctccg gcacttttct
     3961 ccctgcaaac ttcagcccct ggcttgtccc ccccaagccg ggggctcttt tgcatggtgc
     4021 gccacgaacc attgaagaca agcgccagtc cgctcgggct ttagcgcgac acgggctagc
     4081 tggggatatc gttatctaac cgctttttct ggcacgccga tcttagcgtt ttgtcaccgt
     4141 tggatagacc agggattgat cctccatctc ctggatgggg agcttgctta cagcagccat
     4201 cgcatggaga tcttctagcg gcggcatcgc tgccgtcgat aggacaactg aaggggacag
     4261 ggcaatcgag ggggatcgcg aatgcgggaa gaagatcgcg gccacaatca gggcggcaac
     4321 cgcaagagct gaaatcgtca gacgcttcat gggacgcctc catctccccc cgcgataagt
     4381 gggtcgcggg aaccgctcga aagttcaaag gcgcgtcaat gccgcgctag ggtctggcgc
     4441 tgggttcggc gaacgccacc gagacaggtt cctgcacgaa atagtcaagg acgaccgc
//
LOCUS       DATMPU010000275         3279 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_4224898,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000275 DATMPU010000000
VERSION     DATMPU010000275.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 3279)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3279)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3279
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4224898"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1052
                     /locus_tag="VNY06_06320"
     CDS             <1..1052
                     /locus_tag="VNY06_06320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020173493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HWX14456.1"
                     /translation="GYVYSRYSNPTVDMFQERMALLEGAEAARATSSGMAAVTTALLA
                     QLRAGDHVVAARAMFGACLYVVEELLPRFGVASSLVDGADLTQWRAAMRPQTKLLFLE
                     TPTNPCLEVYDLAAIAEIAHQNGARLVIDNVFATPILQKPLKLGADCVVYSATKHIDG
                     HGRCLGGVILGGRELIENEIHNFYKQIGTCLSPFNAWVFLKSLDTLALRVRQQAQNAE
                     QIAEYLSGHRHALKVRYPGRPDHPQAELVKRQMSAGGTLISFEVRGGKPAAFAFANQL
                     SIITIANNLGDAKSLITHPATTTHQRLTPEARAALGVTDGLVRLSVGLEDASDLIADL
                     DGALTVLDRQDLAAE"
     gene            1266..1586
                     /gene="trxA"
                     /locus_tag="VNY06_06325"
     CDS             1266..1586
                     /gene="trxA"
                     /locus_tag="VNY06_06325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020175126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HWX14457.1"
                     /translation="MSTIKVTDANFTSEVEGAKGPVVVDFWAEWCGPCKMIAPALEEI
                     ATELEGKVTIAKLNVDENPKIAGTYGIRSIPTLMLFKDGEKASSKVGAAPKSELKRWI
                     TEAI"
     gene            1736..2371
                     /gene="queE"
                     /locus_tag="VNY06_06330"
     CDS             1736..2371
                     /gene="queE"
                     /locus_tag="VNY06_06330"
                     /EC_number="4.3.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018273385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase"
                     /protein_id="HWX14458.1"
                     /translation="MAKYAVKEIFRTLQGEGAHSGRVAVFCRFAGCNLWSGHEKDRAG
                     AICHFCDTDFVGMDGSGGGSFADADALAAAIESAWTGGKEDRFVVFTGGEPLLQLDAA
                     LIGAVHRLGFEAAVETNGTLFPPQGLDWICVSPKARAPLVANSGSELKLVFPQQGIMP
                     EDLLGLAFSHFWLQPMDGENLAANTAAAIAYCLDHPRWRLSLQTHKFLGLR"
     gene            2388..2744
                     /gene="queD"
                     /locus_tag="VNY06_06335"
     CDS             2388..2744
                     /gene="queD"
                     /locus_tag="VNY06_06335"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012385153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HWX14459.1"
                     /translation="MKISQAFHFEAAHALPNAGEGHRCRRLHGHSYRVELRLEGPVDP
                     VTGFVADFFAIEDAFKPLLERLDHHYLNDIEGLENPTAENIAIWIWEKMKIVLPELER
                     VIVFETQDCFAEFQGT"
     gene            2840..3226
                     /locus_tag="VNY06_06340"
     CDS             2840..3226
                     /locus_tag="VNY06_06340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX14460.1"
                     /translation="MAKTHRPRRHHVSTTGRNRPLSDPPRFTQAPTHRGRPYQDFPLG
                     PGAPPFRAKLEDLIGKLPDGLDKKMVFHICGDTGGVKHPAIPQLVADAIEADARKNTA
                     NGEVAFFYHLGDVVYYNGQATPFGRP"
BASE COUNT          699 a          925 c          899 g          756 t
ORIGIN      
        1 ccggctatgt ctattcccgc tattccaatc cgactgtcga catgttccaa gagcggatgg
       61 ctttgcttga gggcgcggag gcggcacgcg cgacttcgag cggcatggcg gccgtcacga
      121 ctgctctatt ggcacagctc agggctggcg accatgtggt cgccgcgcgc gccatgtttg
      181 gcgcctgcct ttatgtggtc gaagaactct tgccacggtt cggtgtggct tcgagccttg
      241 tcgatggcgc ggatctcacc cagtggcgcg cggccatgcg tccgcaaacc aagctcctgt
      301 ttctcgaaac gccaaccaat ccttgccttg aagtttacga tcttgcggcg atcgcggaga
      361 ttgcacatca gaatggtgcg cgccttgtta tagataatgt tttcgccaca ccaattttgc
      421 aaaagccgct gaagttgggg gccgattgtg tcgtttattc cgcaacaaag catatcgacg
      481 ggcatggccg gtgcctcggc ggcgttattc tcggtggacg cgaactgatc gagaatgaaa
      541 tccacaattt ttacaagcag atcggcacct gcttgtcgcc gttcaatgct tgggttttct
      601 taaaatcgct tgatacgctc gcccttcgcg tacgccagca ggcgcagaac gctgagcaaa
      661 tagcggaata tcttagcggc caccggcacg cgctaaaagt tcgctatcct ggccgtccag
      721 accatccgca agccgagctt gtcaaacgcc agatgtccgc aggcggcacc cttatctcct
      781 tcgaggtccg cggcggcaaa ccggcggcct ttgctttcgc caaccagctt tccattatca
      841 cgattgcgaa taatcttggc gatgcgaaga gtctcattac ccatccggcg accaccacgc
      901 atcaaaggct gacgcctgaa gccagggccg ccttgggggt cacggacggg cttgtgcgcc
      961 tatcggtcgg gcttgaggat gcttccgacc tgattgcgga tttggatggc gccctcactg
     1021 tgctcgaccg ccaggatctc gctgctgaat gaagcggtct cgtctcgctg cctcgtgacg
     1081 cgattatatc gggatcaaaa tcccgcagct caaaccgtgc aaattaagtc ggtagcacgt
     1141 ctacgttctt tatcctctct tacgccctgg ctttccttgg gttgacgcag cccattcgcc
     1201 tacttacctt agcgaaactt gcgcgccagg aattggtccc gcgctcaggt tcgagagaga
     1261 aacttatgtc cacaattaaa gtcacggacg ccaatttcac ttccgaggta gagggagcaa
     1321 agggtccggt ggttgtcgat ttctgggccg agtggtgcgg tccttgtaag atgatcgcac
     1381 cggcgcttga ggaaattgcc acggagttgg aagggaaggt taccatcgcc aagctcaatg
     1441 tcgacgaaaa ccccaaaatc gctggtactt acggcatccg cagcattccg acgcttatgc
     1501 tgttcaagga tggggaaaag gcatcttcga aagttggcgc cgcgcccaaa agcgagttga
     1561 aaagatggat aaccgaggct atctaaacgc tttactcgtg ctgcaataag caaaaaaagg
     1621 acctcgcacg aggtcctttt ttgcttgggg cacacaacca attctgtcga tatgctcaat
     1681 tttatcgata gagtctcctt cgacaaaccg gcactgctgc catcggaacg ggcgaatggc
     1741 caaatatgct gtaaaggaaa tcttccgcac tttgcagggc gaaggcgcgc acagcgggcg
     1801 cgtggccgta ttttgccgtt tcgctggctg caatctttgg tctggccacg aaaaggatcg
     1861 cgccggggcc atttgccatt tctgcgatac agattttgtc ggaatggatg gttctggcgg
     1921 aggcagtttt gcggacgccg atgcacttgc cgccgcgatt gaaagcgcct ggacgggagg
     1981 caaagaagac cgttttgtcg ttttcaccgg cggcgaaccc ttattgcagc ttgatgcggc
     2041 actgatcggc gcagtacaca ggctcggctt cgaagccgcc gtcgaaacca atggcacgct
     2101 cttcccgccg caaggtctcg actggatttg cgtcagccca aaagcgcgtg cccctctcgt
     2161 cgcaaactcc ggatccgagc tcaaactcgt ttttccgcaa caggggataa tgccggaaga
     2221 cctattgggt cttgctttct cgcatttctg gctgcagccg atggatggcg aaaatctcgc
     2281 ggccaatacg gctgccgcga tcgcctattg tctcgatcat ccgcgctggc ggctcagcct
     2341 tcaaacgcac aaattcctcg gcctgagatg aagttgaagt ctcgcttttg aaaatctcgc
     2401 aagcatttca ctttgaggcg gcacacgctc tgcccaacgc cggcgaaggc caccgctgcc
     2461 ggcggctaca cggccattct tatcgtgtgg aattgcggct cgaaggaccg gtcgaccctg
     2521 ttacaggttt tgtcgccgat ttctttgcca tcgaggatgc tttcaagccg ctgctcgagc
     2581 gattggacca tcattactta aatgacattg aaggtctcga aaatcctacc gcagaaaata
     2641 tcgcgatttg gatctgggag aagatgaaaa tcgtacttcc ggagcttgag cgcgtcatcg
     2701 ttttcgagac gcaagattgc ttcgcggaat ttcaaggaac atgagactgc cttgccccat
     2761 cggaaactgt tgttgtcgtc attttgacat ctcgatgctg atagcaatag cttagccaaa
     2821 aatagtggga tggagagcta tggcaaagac gcatcgcccg cggcgccatc acgtcagcac
     2881 caccgggcga aatcgtcccc tgtcagaccc gccacggttt acccaggcgc caacgcatcg
     2941 ggggcgtccc tatcaagact ttcctttggg tcctggtgct ccgccctttc gcgcgaaatt
     3001 ggaggatctt atcggcaagc ttcccgatgg gctcgacaag aaaatggtct ttcatatttg
     3061 cggcgatacc ggcggggtga agcatcccgc gataccgcaa ctcgtggccg atgcgattga
     3121 agccgacgct aggaaaaaca cggcaaacgg cgaggtcgcg ttcttctacc atctcggcga
     3181 tgtcgtctat tacaacggtc aagccacgcc ctttggtcgt ccttgacctc accgccctcc
     3241 ggcacattgg tgtagaaccc gatgaaattt gcaatgggc
//
LOCUS       DATMPU010000276         4481 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_2087614,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000276 DATMPU010000000
VERSION     DATMPU010000276.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 4481)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4481)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4481
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2087614"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            443..1888
                     /locus_tag="VNY06_06345"
     CDS             443..1888
                     /locus_tag="VNY06_06345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012592869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-6-phosphate synthase"
                     /protein_id="HWX14461.1"
                     /translation="MSNRVAAPSAAVTAGGLAVALESVLKEHGGVWFGWSGETCKESE
                     PVLRTTTNGRITFAVSDLSQSDIKGYYHGFANRALWPVCHYRLDLADLSENNTAAYFR
                     VNLQFAVQLHKMLRREDIIWVNDYHLIPIASYLRKLGCRNRIGFFLHIPWPGPDVASA
                     LPGYEQLLRTFGAYDVAGFQTETDASNFFACIMNAKASRSIGQYGCEFGSRRLQVGVF
                     PISIDVGAFAKEARSAERNVIVKRTVGSLDGRPLILGVDRLDYSKGLKQRMEAFAFFL
                     ERSQWAMQTRVTMLQITPKSRSQIPEYVSLQRELAEEAGRINGKFGDVDWVPLRYIHR
                     TLSRAALAGLYRIARVGLVTPLRDGMNLVAKEYVAAQAPDNPGVLVLSQFAGAAHELK
                     SALIVNPYDIEATAAAIARACTMPLDERKDRWRDLIAVLRASSIHNWTEHFLDALGDG
                     AEAGENSKLLLGGVAALGRRPSAARVPALTN"
     gene            2015..3091
                     /gene="recA"
                     /locus_tag="VNY06_06350"
     CDS             2015..3091
                     /gene="recA"
                     /locus_tag="VNY06_06350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012592482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HWX14462.1"
                     /translation="MPLANLRVLEGTSVDKTKALDAALSQIERAFGKGSIMRLGKNQK
                     AVEIDTVPTGSLGLDIALGVGGLPRGRVIEIYGPESSGKTSLTLHVIAEAQKKGGVCA
                     FIDAEHALDTVYARKLGVNLEDLLISQPDTGEQALEITDTLVRSGAVDVLVIDSVAAL
                     TPRAEIEGEMGDSQPGLQARLMSQALRKLTASIARSQTTVIFINQIRMKIGVMYGSPE
                     TTTGGNALKFYASVRLDIRRIGSIKEHDEVTGNQTRVKIVKNKVAPPFKQVEFDIMYG
                     EGISKTGELIDLGVKAGVVEKSGAWFSYDSQRLGQGRENAKNFLKNNPQIAERIEQEI
                     RENSGLIAERILENAEMSGEAEEP"
     gene            complement(3226..3432)
                     /locus_tag="VNY06_06355"
     CDS             complement(3226..3432)
                     /locus_tag="VNY06_06355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX14463.1"
                     /translation="MAVKAFSFAEEKSGGKELPDQSEMSQFGQASNASFRLYSPIGMM
                     DAAAFAVPGTRLALTQRLLGLRQI"
     gene            3570..>4481
                     /locus_tag="VNY06_06360"
     CDS             3570..>4481
                     /locus_tag="VNY06_06360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015931955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase-related protein"
                     /protein_id="HWX14464.1"
                     /translation="MSGVNEIRSAFLDFFQKNGHEIVPSSPLVPQNDPTLMFTNAGMV
                     QFKNLFTGAEKRDYTRAATAQKCVRAGGKHNDLDNVGYTARHHTFFEMLGNFSFGDYF
                     KPLAIDLAWQLITREFGLSPDRLLVTVYHDDDEAFALWKKIAGFPDSKIIRIASADNF
                     WAMGDTGPCGPCSEIFYDQGEKLQGGPPGSPNEDGDRFLEFWNLVFMQFEQVSPGHRI
                     ALPRPSIDTGMGLERIAALLQGVTSNYDIDLLRSLILAVESATDVDPDGPLKASHRVI
                     ADHLRASAFLVADGVLPSNEGRGYVLRR"
BASE COUNT         1015 a         1250 c         1241 g          975 t
ORIGIN      
        1 cgaattggca aacggatggc agcagagaaa acggctcggt gccctttcgg ccatctatac
       61 acgaggcttg cggcatcaat tgtgatgaag tctgtcacaa tttcatatcc gttagtcgac
      121 gagcaaattg ccgcctgcag cactgcaaac ctatgccaga ttgacgtatt tttgacacgc
      181 cccttgccgg ttttttcgta gtttcagcaa tatagaaacg tgtttcagcc tcaccgtcgc
      241 catcgcgatt gtgtattaag attaattaga gtatgcttag cgtcttggtt gtcctgccgt
      301 tgtaatctcg caactaagcg tcttcttatg tgtatataat tatacgctta tcagaagaag
      361 gccgtcaccc atggatcacc ttgaacttct gaataaatcg gcgacagtaa tccctcacca
      421 gacgcgcggc aggcttgtta ttgtgtcaaa tcgcgtggcc gcgccatcgg cggcggttac
      481 ggccggagga ctcgccgttg ccctcgagtc cgtgctcaag gagcatggcg gagtatggtt
      541 cggctggtca ggcgaaacct gcaaggaaag cgaacccgtt ctgcgaacaa ccacaaacgg
      601 aagaattacc ttcgcggtgt ccgatctctc gcaaagcgat atcaagggat actatcatgg
      661 ctttgccaac agggcgctat ggccggtctg ccattatcgc cttgatcttg ctgatctgtc
      721 ggagaacaac acggccgcct acttccgtgt caacttgcag ttcgccgtcc aactgcacaa
      781 gatgctgcgc cgggaggata ttatttgggt gaacgactat cacctcatcc cgatagcgag
      841 ctatttgcgc aaactcggct gccgcaaccg gatcggtttt ttcttgcaca ttccctggcc
      901 gggaccggat gttgcgagcg ccttgccagg ctacgagcaa cttctgcgca ccttcggcgc
      961 ttatgacgtc gctggttttc agaccgaaac cgacgcaagt aactttttcg cttgtatcat
     1021 gaacgccaag gcgagtcgga gcattggcca atatgggtgc gaatttggca gccggaggct
     1081 tcaggtcggc gtctttccga tcagcatcga cgtcggggct tttgcaaagg aggcgcgaag
     1141 tgccgaaaga aatgtgattg tcaaacgcac ggttggaagc ctcgatggcc ggcctctcat
     1201 tctcggcgtt gataggctag actactcaaa gggcttgaaa cagcggatgg aagcatttgc
     1261 ctttttcctt gagcgttcac aatgggcgat gcagacgcgc gtgaccatgt tgcagattac
     1321 gccgaaatcg cgctcgcaga ttccagaata tgtcagtctg caacgtgaac tggccgagga
     1381 agctggccgc atcaacggca aattcggcga cgtcgattgg gttcccttgc gctacatcca
     1441 caggacgctg agtcgcgcgg cgctggccgg gctttatcgt atcgcgcgcg tcggccttgt
     1501 gacaccgttg cgcgacggaa tgaatctggt ggccaaggaa tatgtggcgg cgcaagcacc
     1561 agataacccg ggggtgctcg tcttgtcgca attcgccggt gcagcgcacg agctcaaaag
     1621 cgccttgatt gtcaacccct acgacattga ggcaactgca gccgccattg cccgcgcttg
     1681 caccatgccc cttgacgagc gcaaggaccg ctggcgggac ttgatcgccg tgctccgtgc
     1741 cagcagcatc cacaactgga cggaacattt cctggatgcc cttggcgacg gcgctgaagc
     1801 gggggaaaat agcaagcttc tgcttggcgg tgttgcagcc ctcggccgcc gcccttccgc
     1861 cgcgcgcgtg ccggcgctca ccaattaacc aaacaataaa tggctgatga gtgaacacaa
     1921 aaagtacttg tcaacaggaa caaagagggt accattcatt taacgcagca gacgttgcgt
     1981 agcccctttg ctcatgccag aaggaaagat caccgtgcct ctcgccaacc tccgtgtcct
     2041 ggaagggacg tccgtggaca aaaccaaggc gctcgacgcc gcattgtcgc agattgaacg
     2101 ggcttttgga aagggctcaa tcatgcggct cggcaagaat caaaaggcag tcgagatcga
     2161 cacagtaccg acaggatctc tcggcctcga tatcgcgctt ggagttggcg gcctcccgcg
     2221 cggcagggtc attgagatct atggccccga atcctcgggc aaaacctccc tgactttgca
     2281 tgttatcgcc gaggcccaga aaaagggcgg tgtgtgtgct ttcatcgacg ccgaacacgc
     2341 ccttgacacc gtctacgccc gcaagctcgg ggtcaatctc gaagatcttt tgatttcgca
     2401 gcccgatacc ggcgagcagg cgctcgaaat caccgatact ttggtgcgct cgggcgcggt
     2461 cgatgttctc gtcatcgatt cagtcgcggc gctcacgccg cgcgcagaga tcgagggcga
     2521 aatgggcgat agccagcctg gtctccaggc aaggctcatg agccaggcct tgcgcaagct
     2581 caccgcctcg attgccaggt cgcaaacaac ggtcatcttc atcaatcaaa ttcggatgaa
     2641 gatcggcgtc atgtatggca gcccagagac cacaaccggc ggcaatgcgc taaaatttta
     2701 tgcctcagtc agactagata ttcgccgcat cggctcgata aaggaacatg acgaggttac
     2761 gggaaatcaa acccgcgtaa aaatcgtaaa gaacaaggtc gcgccgccgt ttaagcaggt
     2821 cgaattcgat attatgtacg gcgaaggcat ttcaaagaca ggcgagctca tcgatcttgg
     2881 cgtaaaggct ggcgtcgtcg aaaaatccgg ggcatggttt tcttatgaca gccagcggct
     2941 cgggcaaggc cgggaaaatg ccaagaattt tctcaaaaac aatccgcaga tcgccgagcg
     3001 gatcgagcaa gaaattcgtg aaaattccgg gctcatcgcc gagcgcattc tcgaaaacgc
     3061 cgagatgagc ggagaggccg aggagccatg atgctaggat ttactgcacg gttgagttac
     3121 tgcctccagt agaaatcttc gcgcctggcg tgaatttgcc aggcgcgtta cctttttttg
     3181 cacgcagccg tgccccgcag aatgaatgaa ggccgaagca acttactaaa tttgccgtag
     3241 acccaatagg cgctgagtca aagccaggcg cgtccctggc acagcgaaag ccgccgcatc
     3301 catcatgcca atcggcgagt ataggcggaa actggcattg ctcgcttggc caaattgaga
     3361 catttcggac tgatccggca attccttccc gccggacttt tcctcagcaa aggaaaatgc
     3421 tttaaccgcc acggcgtttc cttcgatagc agagctggaa gactgtaagc tccgcgcatt
     3481 ggagcctggc gccggcgggc accgcgcccc gagcgactgc ccggggccgc gtcgccaaac
     3541 gccgagatca ggattgtgag aatgacgaaa tgagcggcgt caacgaaatc cggtcagcct
     3601 ttctcgattt cttccaaaag aacggacacg agatcgtgcc ctcttcgccg ctcgtaccgc
     3661 aaaacgaccc aaccttgatg ttcaccaatg cgggaatggt tcaattcaag aaccttttta
     3721 ccggggcaga gaagcgcgat tatacccgcg cggcgactgc gcaaaaatgt gtgcgggccg
     3781 gcggcaaaca caatgacctc gacaatgttg gctatacggc ccggcaccac acattttttg
     3841 agatgctcgg caatttttcc tttggcgact atttcaagcc gcttgccatt gaccttgcct
     3901 ggcaattgat cacccgcgaa ttcggcttgt cgccagatcg cctccttgtc accgtctatc
     3961 atgacgacga tgaagccttt gctttgtgga aaaagattgc tggatttccg gattcaaaaa
     4021 tcatccgcat cgcgagtgcg gacaatttct gggcaatggg cgacaccggc ccttgcggtc
     4081 cctgttccga gattttttat gaccagggcg aaaagttgca aggaggcccg ccgggcagcc
     4141 caaacgaaga cggcgaccgc tttctcgaat tttggaacct cgtgttcatg caattcgagc
     4201 aggtttctcc cggccaccgc atcgcgttgc ctcggccgtc gatagatacc ggcatggggc
     4261 tcgagcgtat cgccgcgctt ctgcaggggg tgacgtcgaa ttacgatatc gatcttttgc
     4321 gcagtttgat tctcgccgtc gaatcggcaa cggacgtgga cccagatggc ccgctcaagg
     4381 caagccatag ggtgattgcc gatcatcttc gcgcctctgc atttcttgtt gccgatggcg
     4441 tcttgccgtc gaatgaagga cgcggctatg tgctgcggcg g
//
LOCUS       DATMPU010000277         2026 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_1154980,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000277 DATMPU010000000
VERSION     DATMPU010000277.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 2026)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2026)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2026
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1154980"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..237
                     /locus_tag="VNY06_06365"
     CDS             <1..237
                     /locus_tag="VNY06_06365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX14465.1"
                     /translation="YHPANSPPWALWGVVLCQLLSIVLTVLFWGRWQAQLARDLRGSG
                     SPFLARILKTHWVRTLLTNAYAMILLVWAIAVLS"
     gene            439..1935
                     /locus_tag="VNY06_06370"
     CDS             439..1935
                     /locus_tag="VNY06_06370"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019404160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HWX14466.1"
                     /translation="MPAVENLAGRTFLSGSADPAFRLAPHNIEAEQALLGAILVNNDA
                     FDRVSDFLKPDHFSEEIHRRIFDITSQLLRAGKLASPITLKTFLGEQDLGGVTVPQYL
                     ARLAAEATTIINAEDYGRTIHDLAVRRDLIVVGEDIVNAAYDAPVGTSPREQIEEAER
                     KLYSIAETGRYEGGFQRFSEALTTAVDMAAKAYERDGRLSGIATGLTDLDRYMGGLQP
                     SDLVIVAGRPGMGKTALATNIAFNIARAHEFKLNPDGAAETVNGGIVGFFSLEMSAEQ
                     LATRIIAEQAAVPSYKIRRGDISEPEFHRIADAARTMQAIPFYIDQTGGLSIAQLAAR
                     ARRLKRQRGLDVLVIDYLQLLGASRTRSDSRVQEVTEITTGLKALAKDLNVPVVALSQ
                     LSRQVENRDDKRPQLSDLRESGSIEQDADVVLFVFREEYYLKMRQPREGTDDFVTWQT
                     DMEKMHGRAEIIIGKQRHGPTGTVELAFDAEVTRFSNLAREESLPEHF"
BASE COUNT          424 a          601 c          574 g          427 t
ORIGIN      
        1 tatcatccag ccaattcacc gccctgggct ttgtggggcg tagtgttgtg tcagttgctc
       61 tcaatcgttc tcacggtttt attctgggga cgctggcagg cacagcttgc acgggatctc
      121 cggggttcgg gaagcccttt cctcgcaaga atactcaaaa ctcattgggt gcggacgctt
      181 ctcaccaatg cgtatgcaat gatcttgctt gtctgggcca tcgcagttct gtcctaatcc
      241 aaaactgccg ctgcggttca cgcaacgtcg cgcgccctgc cagttttttt tgcaaaattt
      301 ttcgccggtg tggatagcgg gagcctctcc cgccggacca aagattccct cctttttgcc
      361 gcgcccgcat gcaacaagag agcacatctt gaactgattc ggcgcctgcc gtgcaaatcg
      421 gtccggtcga gaagccacat gccagcagtt gaaaatcttg ccggaagaac gttcctttca
      481 ggcagcgccg atcccgcgtt ccggctggca ccgcacaata tcgaggcgga gcaggctctg
      541 cttggtgcaa tcctcgtcaa taacgacgcc ttcgaccggg tctccgactt ccttaagccg
      601 gaccattttt ccgaggaaat tcatcggcgg atttttgaca tcacttcgca attgctccgc
      661 gcgggaaaac ttgcctcgcc aatcacgtta aaaacttttc tcggcgaaca agatcttggc
      721 ggcgtgacgg tgccgcaata tctcgcccgt ctcgcggctg aagcaacaac catcatcaac
      781 gccgaggatt atggccgcac catccacgac ctcgcggttc gccgtgatct cattgtcgtc
      841 ggcgaggata tcgtcaatgc cgcctatgac gcgccggtcg gcacctcccc gcgcgagcaa
      901 atcgaagagg ccgagcgcaa gctttactcg atagcggaga ccggccgcta tgagggcgga
      961 tttcagcgct tctcggaggc cttgacgacg gccgtcgaca tggcggcaaa ggcttatgag
     1021 cgcgacgggc ggttatccgg catcgcgacg ggccttaccg atctcgaccg ctatatgggc
     1081 gggctgcagc cctcggatct cgtgatcgtc gccgggcgtc ccggcatggg caagacggcg
     1141 cttgcgacca acatcgcctt caatatagcg agagcccatg agttcaaact caaccctgat
     1201 ggagcagcgg aaaccgtcaa tggcgggatc gtcggttttt tctcgctcga aatgtcagcc
     1261 gagcaattgg cgacacgcat cattgccgag caagcggctg tcccctccta caaaatccgc
     1321 agaggcgaca tcagcgagcc cgaattccac cgcatcgcgg atgctgcccg aacgatgcag
     1381 gcgattccct tctacatcga ccagacgggt gggctctcga ttgcccaatt ggcggcgcga
     1441 gcgcggcggc tcaaacgcca gcgcggtctt gatgtcttgg tcatcgacta tctgcaattg
     1501 cttggcgcct caaggacgcg cagcgacagc cgggtccagg aagtgactga aatcaccact
     1561 ggcctcaagg ccttggccaa agatttaaat gttccggtgg tcgcgctctc gcaattgtcg
     1621 cggcaagtgg aaaaccgcga cgacaagcgc ccgcaactgt ccgatcttcg cgagtcgggc
     1681 tcgattgagc aagacgccga tgtcgttctt ttcgtatttc gcgaagaata ttatttgaag
     1741 atgaggcagc cgcgcgaggg aacagacgac ttcgtgacct ggcagaccga catggagaaa
     1801 atgcatggcc gggccgaaat tattatcggc aagcagcggc atggtccgac aggcacagtg
     1861 gaacttgcat tcgacgcgga agtgacgcgg ttttcgaatt tggcgcgcga ggaaagcctg
     1921 ccggaacatt tttagctggc gccgcgcaca tttggggcct agaccgggca ccacgcgaaa
     1981 gtgacgcgca aagaatttct tgccgctgtg cgctaacccc aagctt
//
LOCUS       DATMPU010000278         2075 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_1758918,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000278 DATMPU010000000
VERSION     DATMPU010000278.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 2075)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2075)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2075
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1758918"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(422..1117)
                     /locus_tag="VNY06_06375"
     CDS             complement(422..1117)
                     /locus_tag="VNY06_06375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012589305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HWX14467.1"
                     /translation="MPTIALVDDDRNILDSVAIALEGEGYRVQTYTDGSTALDDLKSN
                     PPDLAIFDIKMPRMDGMELLRRFRQKSDVPVIFLTSKDEEIDELFGLKMGADDFIRKP
                     FSQRLLVERVKAILRRANPKEPGTQKESEAKILERGLLKMDPERHACTWKNDAVTLTV
                     TEFLILQALATRPGIVKSRNALMDAAYDDQVYVDDRTIDSHIKRLRKKFKVVDDEFEM
                     IETLYGVGYRFKE"
BASE COUNT          446 a          612 c          569 g          448 t
ORIGIN      
        1 gcgcgcgcaa acccgcgcag gcgcaggcca acacttcggc gcatgcgctt ggcccaccgc
       61 gccgtgcggg gcgctcgttc gaagcccccg cgtgcttcga gactcattgt ccggcgatcc
      121 ccatccctga ggcgtaaagt gtcatgccta agcccgaaat ggcgaccgga tcccgtcccg
      181 gttcggaccg ataacaggaa catcggtgct tcataccgca tttataccgc gcataacgac
      241 ggatcgcctc gccacggtct ttggcgaaaa gcctgaagcg ctcgaaattt cttgcattgg
      301 cgcgatttat tgcgaaactg actacccgtt ccaccgatca tgctctgaac tgcggtgccg
      361 gttgcgaggg atgcgccctc gtccgccagc ctaaagcgat tgacgtgtgc cggggccaag
      421 atcattcctt gaagcggtag cccacgccat aaagcgtctc gatcatttcg aactcatcat
      481 cgacgacctt gaatttcttg cgcaatcgct taatgtggct atcgattgtg cggtcgtcga
      541 cgtagacctg atcgtcatag gccgcatcca tcaaggcatt gcggctcttg acgattcccg
      601 gtcttgtcgc gagcgcctgg aggatcaaga attcggtgac cgtcaaggtc acggcgtcgt
      661 tcttccaggt gcaggcatgg cgctccgggt ccatcttcaa aagaccgcgt tcgaggattt
      721 tcgcctcgga ttccttctgc gttccgggtt ccttgggatt ggcccggcgc aaaatggcct
      781 tgacacgctc gaccagaagc cgttgcgaga agggtttgcg gataaaatcg tcggcgccca
      841 ttttgagccc gaacaattcg tcgatttcct catcctttga ggttaaaaag atcaccggca
      901 cgtcggattt ctgccgaaaa cggcggagca attccatgcc gtccattcgc ggcatcttaa
      961 tatcgaaaat cgcaagatcg ggaggattgc tcttcaagtc gtcgagcgcc gtcgatccat
     1021 ccgtataggt ctgcacgcga tagccttctc cttcgagggc tatcgccacg gaatcgagta
     1081 tgttgcggtc gtcatcgacc agggcaatcg ttggcatgaa tggtcctaat cacgaattcg
     1141 agcgccacga tcaagtttgc gatgggcttg gccgaaaacc ccttgaaccg ttgccgaagg
     1201 tttcatgcct tttgcagcga attgcggcgc cgccgcattg tcaaagtgtc cgcggccgcg
     1261 acggggcgta acgttgaatt cagcgtcact ccgaggccac atagaaatac ctaccttcca
     1321 aaaaagatcg cgagcgccgc acgctccctc aacctaatgg gcgctggcca ggccggcgtc
     1381 ttcacgaatg gttacgtacg gctcagcttt tggttggagc ggataacctc gtctccctat
     1441 atcaggagaa atgcgcggct gaaataaggc agtctttgga tgcctttccg caatgtccgg
     1501 ttttttgcaa ataagccttt cttctcgatg cggcgccccg cgccgccgag cccaggctga
     1561 aatcaagcag aagtttaaaa aaccaaaaat atcatatact tacaatagcc caattggcaa
     1621 aaaaacgcgg cgtccgtggg gctgctcccc tcgatccaga taaagctgcg gagatagggt
     1681 ggcgccaaga catgagtcgt gtttggactc gggcgactcc ccgaaaaatc cgtttttaga
     1741 cgtaacttgg gaggcgggac ggctcgaagc caagccggtc gacaaggcgg gcgggttcga
     1801 tcggccggac ccctacgcca atcccaaaac gccggcaaca ttcatttctc aggggaaaca
     1861 ggctcggagc gataccggaa gagccgattt ggaaagtctg ttcggactta atcctggcaa
     1921 attagaccgc gcgtcacata gaccctcccg tcgggaccga ccattcgggt gaccctgacg
     1981 caatcctcgt cctcgcctgc cgccgggcgg ccaagctgaa acgccggacc cttaccaagc
     2041 ggcgcataga ccgtttttgt ctcgctcgtc ggctg
//
LOCUS       DATMPU010000279         3078 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_4539759,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000279 DATMPU010000000
VERSION     DATMPU010000279.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 3078)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3078)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3078
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4539759"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1940)
                     /locus_tag="VNY06_06380"
     CDS             complement(<1..1940)
                     /locus_tag="VNY06_06380"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA-associated domain-containing protein"
                     /protein_id="HWX14468.1"
                     /translation="MATTTVNAVDTLLDLRGVTKAYRLETKQFLAVKDINLQIKSGEF
                     VCLLGPSGCGKSTLLRIIAGLNAATSGVVSYDGRPLKGVNPYTTIVFQTFALYPWLTV
                     QENVEIALKARGVPADARTQRALKLIDVVGLDGFESAYPRELSGGMRQKVGFARAMAV
                     EPELLCLDEPFSALDVLSAEALRGELMELWLKKKIPTKAILMVTHNIEEAAFMADRIV
                     IMSKDPGQIVTEIAVTLRHPRHRKDTAFQNLVDNVYAAVAGPPKSKEEALGTQPGQPG
                     KTRPLPNAQLNALAGLLEKLVEEDGRVDLYRMGGDLVLELDDLLPIVEAGDLLGFITL
                     HEGDLLLTPLGRAYADATILARKAIIAGRVLRLPVIAWIYETLRHDDNRRVARDYFHD
                     KLQADFGDKTEEQLDVAISWGRHAELFAYDHDTGELYLESHDGKMAEESIVLTELYEA
                     WPVLSIKERVEGFELVQQDHAEDFFLHLSAGDKAQLVLALPPAERKLWMRLLIPDEAV
                     GVIQEAPEEERERLLSLLDDKTRREVKGLLDYAEEQARGLMHQRYARLRPDMTVDEAV
                     SFLRRDARDRAQDIYYAYVTDSEERLLGIVSFRDLLITPGDKTVQDVMRTDVITAPEH
                     FDQEALRRLFDQYNLQMIPVVDS"
     gene            complement(1960..>3078)
                     /locus_tag="VNY06_06385"
     CDS             complement(1960..>3078)
                     /locus_tag="VNY06_06385"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HWX14469.1"
                     /translation="MAAEIFHVGSRDFRLAGLGSYLQTAANEGNLNAVLLGVVTLVAV
                     IVVLDQLLWRPLIAWTDKFKMDMVEGDEPPRSWFLDLLSRAWLVEQFGTRLWRPFSEW
                     VDRGFQRRTGGVIASPERSAEAGQSRLAVAILTAFLLLALYGSYRAAALLVTLPVSTW
                     AELGKGIVATFLRVSVALAITLIWTIPLGFFIGTNRRLATILQPIVQVVASIPATALF
                     PIVLLALLQAPGGLNIAAVLLMLMGTQWYLLFNVIAGAAAIPQDLRYTTDLLRLSSLD
                     RWRTLILPALFPYIITGSITASGGAWNASIVAEYVEFGGQTHATTGAGALIAEATAKG
                     NYALLLAATLALVLTVVLINRTFWRRLYRLAEERYRLE"
BASE COUNT          713 a          885 c          837 g          643 t
ORIGIN      
        1 gagtccacga cggggatcat ctgtaaattg tattgatcga aaaggcggcg gagcgcttct
       61 tgatcaaagt gctccggggc tgtgatcacg tcggtgcgca taacgtcctg aaccgtcttg
      121 tcgccgggtg ttatgagcaa atccctgaaa gagactatcc ccaacagccg ttcttcagag
      181 tcggtcacgt aagcgtaata tatatcctgg gcccggtccc gcgcgtccct gcgtagaaag
      241 ctaacagcct cgtcgaccgt catgtcaggt cgcaaacggg cgtagcgctg atgcatcaga
      301 cctctggctt gttcctccgc ataatccaaa agtcctttga cttctctgcg ggtcttgtca
      361 tcgagcagcg ataagaggcg ctccctttct tcttcaggcg cttcttggat tacgcccacg
      421 gcttcatcag gaatgagcag cctcatccac agtttccgtt ctgccggcgg tagggctaaa
      481 acaagttggg ccttgtcccc ggcactcagg tgcagaaaga aatcttccgc atgatcctgt
      541 tggaccaact caaagccttc gactcgctcc tttatagaaa gcacaggcca ggcctcgtaa
      601 agctcggtca aaacaatgct ttcctcagcc atttttccgt catgactctc tagatataat
      661 tctccggtat catggtcata ggcgaaaagc tcagcgtgcc gtccccaact aatggcgacg
      721 tcgagttgct cttcggtctt gtcgccaaaa tcggcttgca gcttgtcatg gaaataatcc
      781 cgagccaccc gccgattgtc atcgtggcgg agcgtttcat agatccaggc gataaccggc
      841 aatcgcaaaa cccggccggc gatgattgct ttgcgcgcca ggatggtagc atcggcgtat
      901 gcgcgtccaa gcggggtgag cagcaaatct ccttcatgca gtgtgatgaa acccagcaga
      961 tctcctgcct ccacaatggg caagaggtca tcgagctcga gaaccaggtc gccgcccata
     1021 cggtagagat cgacccgtcc atcttcctcc actagtttct ccaacaggcc ggcgagggcg
     1081 ttcagttggg cgttgggcaa cggccgggtt tttccaggct gcccgggttg agttccgagc
     1141 gcttcctctt tcgatttggg tggaccggca acggcggcgt aaacgttatc gaccagattt
     1201 tggaacgccg tatccttacg gtggcgcgga tggcgaaggg taactgcaat ctcagtgacg
     1261 atttgaccgg gatctttgct catgatcact atccggtccg ccatgaacgc agcctcctcg
     1321 atgttgtgcg tgaccatcaa aatggctttt gtggggattt ttttctttag ccagagttcc
     1381 atcaactcgc cacgcaaagc ctcggccgaa agcacgtcca aggcggaaaa cggttcgtcc
     1441 aaacacaata attccggttc caccgccatg gcacgggcga agccaacttt ctgccgcatg
     1501 ccgccggaaa gttcgcgcgg gtaggcggat tcgaatccgt ctagaccgac aacatcaatc
     1561 agctttagag cgcgctgggt gcgcgcgtcg gccggcacgc cgcgcgcctt caatgcaatt
     1621 tcgacattct cctgcaccgt cagccaggga taaagcgcaa acgtttgaaa aacgattgtc
     1681 gtgtacggat tgaccccttt cagcggccgg ccgtcatatg agaccacgcc ggacgtagcg
     1741 gcgttcaatc ctgcaatgat ccgcaagagc gtcgattttc cgcatccgga tgggcccagc
     1801 aaacagacaa attcacccga cttgatttgc agattgatgt ctttgacggc aagaaattgc
     1861 ttggtctcta accggtaggc tttggtgacg cctcgaaggt ccaacaacgt gtcgacggca
     1921 ttgactgttg tcgtagccat agcaagcctc gggtatagat tattccagac ggtaacgctc
     1981 ttcagccaat ctatagagac gccgccagaa ggtccggtta atcagaacaa cggtgagaac
     2041 cagagccaac gtggctgcca gcagcagcgc ataattgccc tttgcggtgg cttccgcgat
     2101 gagcgcgcca gctccggttg tggcgtgcgt ttggccgcca aattccacgt attcagccac
     2161 gatacttgcg ttccaggcgc cgccgctcgc ggtgatggat ccagtgataa tgtacgggaa
     2221 gagcgcgggc aggatcagag tccgccagcg gtccagagag gagaggcgca gcagatcggt
     2281 ggtataacgt agatcttgcg ggatagccgc tgcacccgca attacattaa aaaggagata
     2341 ccactgcgtt cccatcagca tgagaagcac cgcggcgata ttcaaaccgc ccggcgcctg
     2401 caacaacgcc agcagcacga tgggaaaaag cgcggtcgcc ggaatggacg caacgacttg
     2461 cacgatcggt tggagaatgg tcgcgaggcg gcggttcgtt ccaataaaaa agccgagtgg
     2521 aatggtccag atcaaggtaa tcgccagtgc gacgctcacc cgcaggaaag tagcgactat
     2581 tccctttcct agttcggccc atgttgaaac gggaagcgta accaacaacg cggctgcccg
     2641 ataacttcca tataaggcca gaagcaagaa agccgtcaag atggcaaccg ccaatcgcga
     2701 ttgtcctgcc tcagcggacc gctccggtga cgcgatgact ccaccggtgc gtctttgaaa
     2761 ccctctgtcc acccattcac tgaaaggtcg ccataggcgc gtgccgaatt gctccaccag
     2821 ccaagcacgc gaaaggagat cgagaaacca cgagcgcggc ggctcatcgc cctcgaccat
     2881 gtccatcttg aatttgtcag tccatgcgat gaggggccgc cacaaaagct ggtcgagcac
     2941 aacaataacc gcgaccaagg tgaccacgcc caaaaggact gcgttcaaat tcccctcgtt
     3001 agcggcggtt tgcaaatatg agccgagacc ggccaagcga aaatcgcggc tgccgacgtg
     3061 aaaaatctcc gcggccat
//
LOCUS       DATMPU010000280         3266 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_1155579,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000280 DATMPU010000000
VERSION     DATMPU010000280.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 3266)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3266)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3266
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1155579"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..336
                     /locus_tag="VNY06_06390"
     CDS             <1..336
                     /locus_tag="VNY06_06390"
                     /inference="COORDINATES: protein motif:HMM:NF024139.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HWX14470.1"
                     /translation="WLALPACASSHLADFNTAPLMEVELANINDIPKSNDKEQKVPDL
                     ESQAVSVGRKAFGIDEFCQLFGVGRTTTYSEIKVGRLRARKVGARTIIAADDANNWLQ
                     SLPHIRAKP"
     gene            333..1244
                     /locus_tag="VNY06_06395"
     CDS             333..1244
                     /locus_tag="VNY06_06395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011314548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HWX14471.1"
                     /translation="MSAVDIARALGGRKSGSAWMARCPVHEDRTPSMSISDADDGKTL
                     VRCHAGCEQAIVIAALRVRRLWSANDRCQSKIIDPKSSRFLRDGEIADRTAAALKIWQ
                     SAAPATNSVVTDYLQSRGIDITPPNCLRFHPELRHPSGGVWPAMIALVSGSDNKPVAI
                     HRTFLARSGTGKAPVVPTKMMLGPCHGGVVRLGTVHPKNLLVVAEGVETALSVMQACG
                     LPGWAALSANGIRSLTLPPEASTVLICADNDANGVGQRAANEAAERFLREGRQASIAM
                     PPMTGTDFNNVLNGGEKDAIVETRDVA"
     gene            1234..2751
                     /locus_tag="VNY06_06400"
     CDS             1234..2751
                     /locus_tag="VNY06_06400"
                     /inference="COORDINATES: protein motif:HMM:NF024547.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfjI family protein"
                     /protein_id="HWX14472.1"
                     /translation="MSPSGPEAVRKIVGQATEANLEPPRPLIRELPPADPFPVNALGD
                     VLEAATRAINDRVRSPMAICGQSTLAAATLAVQGHADIELPTGQAKPILNFFISIAAT
                     GLRKTATDSEALWPVRKRENALRQDYDRALPDYTNAMMAFEKVRDQVVRRHAKKANRA
                     ELESALEALGPSPIAPLTPMLTCPEPTYEGLCKHLVSGRPSIGIFSAEGGQFIGGHGM
                     AQEAKLRTVTGLSCLWDGEVIKRVRALDGATILPGRRVAVHLMAQPDVAALMLSDPVL
                     INQGLLSRCLVTAPESNAGTRTWREASTASDLAIKRYGGRILDILEKQLPLAAGKTNE
                     LSPRILQLAPDARKMWIAFADNIEGQIGPDGALGPVSGLANKLPEHAARLASVLMLVE
                     DIDACEVSAEKMSAGIILAQHYAAEALRMFEGSRISGDLLLAQKLLGWLNQSWNERAI
                     SLPDIYQKGPNAIRDKATAVKLVRILEDHGWLHRIDGAALIAGHHRREAWLVIKG"
     gene            2756..>3266
                     /locus_tag="VNY06_06405"
     CDS             2756..>3266
                     /locus_tag="VNY06_06405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX14473.1"
                     /translation="MTGFAKFDPLAFLEREKRIVANKDFVQIPNGVFHPTLASLATLA
                     AAPLQKENEDDCQSWTTDDYHHDEKSQKFAADAAKIAKVAKVTARSTSIDYSSWREVE
                     EERAAIAEFDGGAPRVWAEALARLDPARAPRDIMPKRWLCFIDDCGRFLDEGWAARAE
                     ELGWRPLELF"
BASE COUNT          716 a          891 c          976 g          683 t
ORIGIN      
        1 tggcttgctt tgcccgcttg cgcgagtagc catttggccg atttcaacac cgccccgctc
       61 atggaggtgg aattggccaa tatcaacgat attcctaaat ctaatgataa ggagcagaaa
      121 gttccggacc tggagtcgca ggctgtttct gttgggcgaa aggctttcgg aattgatgag
      181 ttctgccagc tttttggcgt cgggcgaacg acgacctact ccgaaatcaa agttgggcgt
      241 ttgcgggcgc gcaaggtcgg agcaagaaca atcatcgcgg ctgacgacgc taataactgg
      301 ctgcaaagct tgccccatat cagggcgaag ccatgagcgc cgtcgatatc gcgcgcgccc
      361 ttggtggtcg aaaatctggt tccgcgtgga tggcacgctg ccccgtccat gaggaccgaa
      421 cgccatccat gtcaatctcc gacgccgacg atggaaaaac acttgttcgc tgtcatgccg
      481 gttgcgaaca agcaatagta atcgcggcgt tgcgagtgcg acgtctatgg tcagctaacg
      541 atcggtgtca atccaagatc atcgacccga agtccagtcg gttcctacgc gatggtgaaa
      601 ttgctgaccg gaccgcagca gcgcttaaaa tctggcagtc cgcagcccca gccaccaata
      661 gcgtggtgac ggactacttg cagtctcgcg ggatcgacat cacgccgccg aactgcctac
      721 gtttccatcc ggaactccgg catccctcgg gtggcgtctg gcccgcaatg attgcgctgg
      781 taagcggcag tgataacaaa cccgtggcga tccaccggac gtttcttgcc agatccggca
      841 caggtaaagc tcccgtcgtg ccgacgaaga tgatgcttgg gccctgccat ggcggggtgg
      901 tgcggctcgg caccgttcat ccaaagaacc tcttggtcgt cgccgagggc gtcgagacgg
      961 cgctgagcgt tatgcaggca tgcggactgc cgggctgggc ggcgttatcg gccaacggca
     1021 tcaggagtct taccctgccg ccagaggcgt ccactgtgct gatctgcgcc gataatgatg
     1081 ccaatggagt agggcagcgt gctgccaacg aggccgctga gcgcttccta cgcgaaggcc
     1141 gtcaagcaag catcgcgatg ccgccgatga caggaacgga tttcaacaat gtcctgaacg
     1201 gcggcgaaaa agacgccatc gtggagaccc gcgatgtcgc ctagcggacc cgaggccgtg
     1261 cgcaaaatcg ttgggcaggc aacggaggcg aatttggaac cgccccgtcc gctgatccgc
     1321 gagcttccac ctgcagaccc gttcccagtc aacgcgctag gtgacgttct agaggccgca
     1381 actagagcca taaacgaccg ggtaaggtct ccgatggcga tatgcggaca atccactctt
     1441 gcagcggcaa cacttgccgt gcagggccat gccgacatcg aattgcccac tgggcaggcg
     1501 aagccaatat tgaacttctt tattagcata gctgcgactg gtttacgaaa aactgcaaca
     1561 gatagcgaag ccttatggcc agtccggaaa cgcgaaaacg cgcttcgcca agattacgac
     1621 agggctttgc cagattacac caatgccatg atggctttcg aaaaagtgcg agatcaggtc
     1681 gtaagaaggc atgcgaagaa ggcaaatcgt gctgaactcg agtccgcgct cgaagctcta
     1741 ggtccatctc ctatcgcacc cttgacgccc atgctgacct gtcccgagcc gacgtatgaa
     1801 gggctctgca agcatctcgt atccggtcgg cctagcatcg gcattttctc cgcagaaggc
     1861 ggacagttca tcggtggaca tggtatggcg caagaggcga agttgcgcac cgtaaccggc
     1921 ctttcgtgcc tgtgggacgg tgaggtgatc aagcgcgttc gagccttgga tggtgcaaca
     1981 atcctgcccg gtcggcgggt ggcagtacat ttgatggctc agccggatgt cgctgcatta
     2041 atgctgtcag atccggttct tataaaccaa ggcctacttt cccgctgttt agttactgca
     2101 ccggagtcaa acgccggaac gaggacatgg cgcgaggctt ctactgcttc cgatttggcg
     2161 attaaacgat acggtggacg cattctcgac atactggaga agcagttgcc gctcgccgcg
     2221 ggcaagacca acgagctttc gccccgcatt ctgcagcttg cgcctgatgc ccgcaaaatg
     2281 tggatcgcct tcgcggataa tatcgaaggc caaatcgggc ccgacggtgc gctcggtcct
     2341 gtgagtgggc ttgccaataa gctcccggaa catgcggcac gtcttgcgtc agtacttatg
     2401 ctcgtcgagg atatcgacgc ttgcgaagtc tcggcggaaa aaatgtccgc cgggattatt
     2461 cttgcgcaac attatgccgc agaagcgctg cggatgtttg agggcagtag gatcagcggg
     2521 gacttactcc tcgcgcagaa gctcttgggt tggctgaatc aatcttggaa tgagcgcgcg
     2581 atatcgctgc cggacatcta tcagaagggt cccaatgcga tccgcgacaa ggcgacggcc
     2641 gtgaagctcg tgaggatact ggaagatcat ggttggcttc atcgtattga tggagcggcc
     2701 ttgattgcag gtcatcatcg ccgcgaggcc tggctggtca ttaagggtta gtgccgtgac
     2761 tggtttcgca aaatttgatc cccttgcctt cctcgaaaga gaaaagcgca tagtcgccaa
     2821 taaggatttt gttcaaattc cgaatggggt ttttcacccg actttagcaa gtttagcgac
     2881 tttagcagcc gcgcccttgc aaaaagagaa tgaggatgat tgtcaatcgt ggacgaccga
     2941 tgactaccat cacgatgaga agagccaaaa attcgcggcc gatgctgcta agattgctaa
     3001 agtcgctaag gttacggcgc ggtcgacttc gatagactac tcgtcgtggc gtgaagttga
     3061 ggaagaacgc gccgccatcg ctgagtttga tgggggcgct ccacgggtct gggcggaggc
     3121 gctggcgcgg cttgaccccg cacgtgcgcc ccgcgacatc atgccgaaac gatggctatg
     3181 cttcatcgac gattgcggac ggttcctgga tgaaggttgg gcagctcgtg ctgaggagct
     3241 gggttggagg ccgctcgagc tttttg
//
LOCUS       DATMPU010000281         4440 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_1759820,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000281 DATMPU010000000
VERSION     DATMPU010000281.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 4440)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4440)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4440
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1759820"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            116..667
                     /locus_tag="VNY06_06410"
     CDS             116..667
                     /locus_tag="VNY06_06410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012591504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HWX14474.1"
                     /translation="MSLVTGKQDVPQHPQRTLGALQEAGSPPMYDLIELMFFAYRDFV
                     GDADRALAALGFGRAHHRLLHFVARHPGLTMAELLDILKITKQSLNRVLKEMVDKNYV
                     EVRPGMKDRRQRQLYPTLRGEALALEVARLQSRRFARVFDSLPEGARPQVQAFLLSLV
                     DPGTRNKIAAMTGVSATFGSSGS"
     gene            670..1410
                     /locus_tag="VNY06_06415"
     CDS             670..1410
                     /locus_tag="VNY06_06415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019400350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HWX14475.1"
                     /translation="MIEEQNGVFAAATPADDAAHLLIIDDDRRIRALLSRMLSAEGYR
                     VTTAADATEAMARLKSISFDLIVLDVMMPGEDGFAFAARLRADRTELAHVPILMLTAR
                     SEADDRVRGLEAGVDDYLGKPCEPRELTLRVASILRRAAPRVSQRPPASVHFGDFTFD
                     LGRGELRQRGEIIRLTDRERAMLRLLAENPGETVARETLSGLGVAANERTVDVQVNRL
                     RRKIERDPSNPLYLQTARGAGYRLLVDH"
     gene            1416..2801
                     /locus_tag="VNY06_06420"
     CDS             1416..2801
                     /locus_tag="VNY06_06420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002717045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWX14476.1"
                     /translation="MTKAHGLRLSFPLTFQQARPWWLALWRKAAAVMPKGLFARAILI
                     VILPMVVLQCVLTYVFMERHWQLVTKRLSTALTQDIAAIIELHETYPGQTNEAILDRI
                     AQHRLKIDVEFLPQGELPPALPKPFFSILDAALSNEIRRQIGKPFWLDTVGRPTFVEV
                     RIMLDDSILRVVALRGAAYASNSHIFLLWMVGTSLVLITVAILFLRSQIKPILLLTDA
                     AEAFGKGRDLEFHPRGAREVRKAGAAFLEMKRRIERAFEQRTAMLNGVSHDLRTILTR
                     FKLSLALMVETDETRDLQKDVAEMQRMLASYLAFARGDLGESAAEIHMEDFLEDLRLD
                     AERAGVEATIALSGDPIVTVRPDAFKRCVANLIGNAQSHGKHIFIEAVRDKRFLTIHV
                     DDDGPGIPPDKREEVFKPFVRLDSARNQDEGGTGLGLAIARDIARSHGGDISLTNSKA
                     GGLRATVRVPV"
     gene            complement(2872..3402)
                     /locus_tag="VNY06_06425"
     CDS             complement(2872..3402)
                     /locus_tag="VNY06_06425"
                     /inference="COORDINATES: protein motif:HMM:NF018269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="di-heme oxidoredictase family protein"
                     /protein_id="HWX14477.1"
                     /translation="MRDDKGNQAFNNPLHILPDWMQFQLLMRFMADPQPDPHPSPSAQ
                     RGRVVFSDVGCGLCHTPQMRTAPAMNSAVLQDRPANLFSDLLVHHMGGRLADDIIQGG
                     AEPDEFRTTPLWGVGQRLFFLHDGRTSDLLAAIKGHFSPKTGPYPLSEANGVIMKFTR
                     LPPPDQQAILDFLRSL"
     gene            complement(3371..4054)
                     /locus_tag="VNY06_06430"
     CDS             complement(3371..4054)
                     /locus_tag="VNY06_06430"
                     /inference="COORDINATES: protein motif:HMM:NF018269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="di-heme oxidoredictase family protein"
                     /protein_id="HWX14478.1"
                     /translation="MLTNVFVPRPADLGGLGRISRSQSGPGHAFAFGKSPVPAVPHRF
                     GKQNIVPSFEEQFGPIREVRFKFKPEGTRDGSVHQLWVVRVITKDPTIPNCAIRQPDF
                     AAELARGNLSFRIPPQLFGLGLIDSIQDREILGRHAATASLRVPLGIEGHPNRSGNDN
                     TITRFGWKAQNKSITVFAGEAYNVEMGISNELFPTFVEEDPACNGAGKTRTERCGPHD
                     EGRQGESGV"
BASE COUNT          855 a         1306 c         1334 g          945 t
ORIGIN      
        1 tggcggagcg ggtgctggaa aaccatgccg aagttgcaaa gccgtcaggc gcggacttga
       61 cgtctaagga ggttgttgaa atatgtcaat ggtgctgacg taaaaggccg tgcgcatgag
      121 tctggtaacc ggcaagcagg acgttccaca acaccctcaa cggaccctgg gcgctttgca
      181 ggaggccggc agcccgccca tgtatgatct gatagagctt atgtttttcg cctaccggga
      241 ttttgttggg gacgcggatc gcgcactggc cgcgctcgga tttggccggg cgcatcaccg
      301 cctgttgcat ttcgtggctc ggcacccggg ccttaccatg gctgagctgc ttgatatttt
      361 gaaaatcacc aagcagagtc tcaatcgcgt cctcaaagaa atggtcgata agaactatgt
      421 cgaggtccgc ccaggcatga aggatcgccg tcagcgccag ctctatccga ccttgcgcgg
      481 cgaggccctc gcgctcgaag tcgcccgact gcagtcgcgg cgatttgcgc gcgtgttcga
      541 cagcctgccg gagggtgcgc ggccacaggt acaggctttt ctcctctcat tggtagaccc
      601 aggcacgcgg aacaaaatag ccgcgatgac cggcgttagc gccacctttg gcagctcagg
      661 atcgtaacca tgattgagga gcaaaatggg gtgttcgcag cagccacacc ggccgatgat
      721 gcggcgcatc ttctaatcat cgatgacgac cggcgcatcc gcgcgcttct ctcccgcatg
      781 ctgagcgcgg aaggctatcg agtcacgacc gccgccgatg ctacggaagc catggcaagg
      841 ctcaagagca tttcctttga tcttattgtg cttgatgtca tgatgccggg cgaggacggc
      901 tttgctttcg cggcgcggct gcgggcggac agaacggagc tcgcgcatgt ccccatcttg
      961 atgctgacgg cacgcagcga agcggacgac cgggtgcgcg gcttggaagc cggcgtcgac
     1021 gactatcttg gtaagccttg cgagccgcgc gagctcactt tgcgggtcgc ctcgatcctg
     1081 cgccgcgcgg ctccgcgcgt ttcgcaacgg ccgccagcct cggtgcattt tggcgatttc
     1141 accttcgatc ttggccgcgg agaattgcgc cagcgcggtg agattataag gctgaccgac
     1201 agagagcgcg cgatgctgcg ccttttggcg gagaacccag gcgaaaccgt cgctcgggaa
     1261 accctttccg gtctcggtgt tgctgccaat gagcgcaccg tcgatgttca ggtgaaccgg
     1321 ctgcgccgca agatcgagcg cgacccgtcc aatccactgt atttgcaaac ggcgcggggc
     1381 gctggctatc ggctgctcgt cgatcactga caccaatgac aaaggcgcat gggttgaggc
     1441 tttctttccc cctcacgttt caacaggccc gcccttggtg gctggcactc tggcgcaaag
     1501 ccgccgcggt catgcccaag ggtcttttcg caagagcaat tctcattgtc atcctgccaa
     1561 tggtggttct gcaatgcgtg cttacttatg tctttatgga gcggcattgg cagcttgtca
     1621 ccaagcggct ctcaaccgca ttaacccaag acatcgcggc aatcatcgag ctgcatgaaa
     1681 cctatccggg ccaaaccaat gaagcgattc tcgaccgcat tgcccagcac cggttgaaga
     1741 tcgacgtcga gtttctgcca cagggcgagt tgccgccggc gctgccaaaa cctttcttct
     1801 cgatcctcga tgccgcactt tccaacgaga tcaggcggca gatcggcaaa cctttttggc
     1861 tcgataccgt gggacgcccg actttcgtcg aggtcaggat aatgctcgat gattccattt
     1921 tgcgcgtcgt tgcgctgcgc ggggccgctt atgcgtcgaa ttcccacatc tttctgttat
     1981 ggatggtcgg cacctctctg gtattgatca ctgttgcgat cctttttctg cgcagtcaga
     2041 tcaagcctat tctgttgctc acggacgcgg cggaggcctt tggcaagggc cgcgatctgg
     2101 agtttcaccc acgcggcgca cgagaagtgc gcaaagctgg cgcagccttc cttgagatga
     2161 agcggcggat cgaacgggcg ttcgaacaac gcaccgctat gctcaatggg gtaagccatg
     2221 atcttcgcac catcctgaca aggttcaaat tgtcgcttgc cttgatggtg gaaaccgatg
     2281 agacccgcga cctgcaaaag gatgtcgccg aaatgcagcg catgctcgcc tcttatctgg
     2341 cttttgcacg cggcgacttg ggcgagagcg cggcagagat ccacatggag gattttttgg
     2401 aggatctacg gctcgatgcc gagcgggccg gcgttgaggc caccattgcc ctgagcggtg
     2461 acccgatcgt caccgtgcgt cccgacgctt ttaagcgctg cgttgccaat ctcatcggca
     2521 acgcccaaag ccatggcaag catattttta tcgaggcggt gagagacaag cgttttttga
     2581 cgatccatgt cgatgacgat ggccccggga ttccgcccga caagcgcgag gaggttttta
     2641 agccctttgt tcgcctcgac agcgcccgca atcaggacga aggcgggacc gggctcggtc
     2701 ttgcgatcgc gcgcgacatc gcccggtcgc atggcggcga tatcagcctt actaacagca
     2761 aggccggcgg tctccgagcc accgtgcgcg tgccggtgtg attgcagccg ttgccgtcgt
     2821 actgacgcgc cacggccgcc gcgcgcgccg ccgtggcgga ttctttatct ctcacaagga
     2881 gcggaggaaa tcgagaatgg cctgctggtc tggtggcggg agccgggtaa acttcataat
     2941 aaccccattg gcttccgaca aaggataagg ccccgttttc ggcgagaaat gccccttgat
     3001 cgcggctagc aaatcgcttg tgcggccatc gtgtaaaaag aacaaacgct gtcccacgcc
     3061 ccaaagcggc gtcgtgcgga actcgtccgg ctccgcgcct ccctgaatta tatcgtcggc
     3121 gagcctgcct cccatgtgat gcaccaagag atccgagaac agattggcgg gccggtcttg
     3181 caggacggca ctgttcatcg ccggcgcggt ccgcatttgc ggcgtgtgac acaagccgca
     3241 accgacgtcg ctgaagacga ccctgccgcg ttgcgcgctc gggctcgggt gcggatcggg
     3301 ttgcggatcg gccatgaacc gcatcaagag ttgaaactgc atccagtccg gcagaatatg
     3361 cagcggattg ttaaacgcct gattcccctt gtcgtccctc atcgtgcgga ccacatcgtt
     3421 cggttcgggt tttccccgct ccgttgcaag ccggatcttc ttcgacgaag gtcgggaata
     3481 gttcgttgct tatccccatt tccacgttat aggcttcgcc agcgaatacc gtgatcgact
     3541 tgttttgcgc cttccagccg aaccttgtga tcgtgttgtc gttgccgctg cggtttggat
     3601 ggccttctat gccaagcggc acgcgcaacg aagccgtggc ggcgtgacgg cctaaaatct
     3661 cccggtcctg aatcgagtcg atcagcccaa ggccgaagag ttgcggggga atccggaacg
     3721 acaggttccc gcgcgccagc tcggccgcga aatctggctg tctgatcgca caatttggga
     3781 ttgtcgggtc tttcgtgatt acccggacca cccagagctg gtggacgctg ccgtcgcggg
     3841 ttccttccgg cttgaacttg aaccgaactt cccgaatggg gccgaactgt tcttcgaagc
     3901 tcgggacgat gttttgtttg ccaaaacgat gcggaacagc cggaacaggg gattttccga
     3961 acgcaaaggc gtgccctggc ccggattggg aacgagaaat ccgcccgagc cccccaaggt
     4021 cggctgggcg tggcacaaag acattggtca gcattgtgcc tggctccaag gccgttagag
     4081 tttgtgtgaa actgagggaa gaaaggatcg agctccccct tgccaacaac cggactgccg
     4141 tcggtcacgt cggcacaggt atcgcaggtc gattccaatt gtccggcccg gttgatgcct
     4201 tcgatgaacg aggcttcttc gttgacttga acctgagccc cagttgccgg gttcgggcta
     4261 atcagcggcg ggtgaggaat ggggatgcct ctgcgttcca gttcccccgc ggtgttctcg
     4321 cgaccgccgc gcactccggg atccggttgc gcacaaacgg ggcccgctat tgtgaatagc
     4381 gacgcggcga aaactgaggt ccaacaatta ttattagtca tgataaccct tacgaatgtg
//
LOCUS       DATMPU010000282         3521 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_2689667,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000282 DATMPU010000000
VERSION     DATMPU010000282.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 3521)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3521)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3521
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2689667"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1320)
                     /locus_tag="VNY06_06435"
     CDS             complement(<1..1320)
                     /locus_tag="VNY06_06435"
                     /inference="COORDINATES: protein motif:HMM:NF012722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HWX14479.1"
                     /translation="MEFLSKLQRWTANASLQAVFSFPFERADETSFVRRQALTALGSG
                     ILAFLWVAEDGVPALSDTMILILLSAPLSIWLLRATRRLFAAEALTSATFIAAGLIAA
                     LSGGALHQTEFLWFVIAPVEGIFSANSAVAAASGILAAMAALIVAVLSKPDALPSPEA
                     AGFIPFLIPVVLLALVVTTGFFRLRALRRSARQVLPENCDNLALTMGCLALRCGLSGR
                     VSAVSPNCESLFGLAPSELTNRGFFDRVQVADRPTFLKAISDASVPSETAVATVRWRG
                     SERVAHENYAGPVYHWLEMRARRSEEYPAAGQEHHDSDVIVFFRDVTEAKRRDSELEN
                     ARADLAEANIAREQFFAHAGHEIRAPLNAIAGFSQLLADPEGPAPEPEKQREYARIIH
                     RSGQHLLAVVNSITDMSLIQSGRLAITLERFAAASQIDLCCDMVGLQA"
     gene            1523..1855
                     /locus_tag="VNY06_06440"
     CDS             1523..1855
                     /locus_tag="VNY06_06440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX14480.1"
                     /translation="MTLLHRPRGLKMLFLIRAIFWLSIVSYFLPWPEDKIALFLTKSD
                     LKSEAQDILGRTVGMAQAAAERECLRVPAACLKEAARLSRMAAGHLMAGKDKAPSLPP
                     VDAPSARH"
     gene            1907..2350
                     /locus_tag="VNY06_06445"
     CDS             1907..2350
                     /locus_tag="VNY06_06445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019400436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SufE family protein"
                     /protein_id="HWX14481.1"
                     /translation="MTGAGIATKLEEIIENFEYLDEWEDRYRYLIELGRGLEPLDAAA
                     YCDKNKVRGCASQVWLETKVASEQQGSPVLYLKGDSDAHIVRGLVALLLALYSGQPAA
                     RILTIDAHGLFEKLGLLAHLTPQRSNGVRSMVERIKSEARTAAAA"
     gene            2718..3344
                     /locus_tag="VNY06_06450"
     CDS             2718..3344
                     /locus_tag="VNY06_06450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX14482.1"
                     /translation="MLKFKSRLTAAAVLALTCLANGPAWAATSVTYVSGKGTDAGTCA
                     APTNPCRTIQFAVNQTAGGEVKALDPADYSPVTINKSISITGGVYFAQKRCVFRIAFG
                     LKKSLDVSCFKLVSVIKQLHRVEPDTIIKMFSCQDPKDAPSGFRKERLAAGENGKPLF
                     IWQIVIKEWSRIAIPQGFHEMAGNLSKPSVHSEFFFDHRNPEEALGFL"
BASE COUNT          823 a          997 c          987 g          714 t
ORIGIN      
        1 cgcctggagt ccgaccatgt cgcaacaaag atcgatttgc gacgcggcgg caaaacgttc
       61 aagtgtgatt gccaatctgc cggactggat caacgacata tccgtgatcg aattgacgac
      121 ggcgagaagg tgctgccccg atcgatgaat gatccttgcg tattcgcgct gcttttcagg
      181 ttcgggcgcc ggcccttcgg gatctgcgag caattgtgaa aaaccggcga ttgcattgag
      241 cggcgcgcgg atttcatgac ccgcatgggc aaaaaactgt tcccttgcga tatttgcttc
      301 cgccaaatcg gcgcgtgcat tttcgagttc cgaatcgcgg cgcttggctt cggtcacatc
      361 gcggaaaaat acgatgacat cgctgtcgtg gtgctcttgc cccgctgctg gatattcctc
      421 gctgcggcgc gcccgcatct caagccagtg gtaaaccggg ccagcatagt tttcgtgcgc
      481 aacacgctcg gagccgcgcc agcgcactgt cgcgactgcc gtctcggatg gaacgctggc
      541 atcggaaatg gccttcaaaa aagtcggcct gtctgcgacc tgaacacggt cgaaaaaacc
      601 gcgattcgtt agctcgctcg gcgccaaacc gaacaagctc tcgcaattgg gacttaccgc
      661 agagacgcgg ccagaaaggc cgcaacgcaa agcaaggcag cccatggtca gggcaagatt
      721 atcgcaattt tccgggagaa cttgccgcgc gctgcggcgt aaggcgcgaa ggcggaaaaa
      781 gccggttgta acgaccagcg cgagcaggac aacggggatt aggaagggaa tgaaacctgc
      841 cgcctctggc gatggcagag catccggctt ggaaaggacc gcaacaatga gcgcggccat
      901 cgccgccaaa atcccgcttg cagccgcaac agcgctgttc gcagagaaaa tgccttcaac
      961 cggcgcgatc acgaaccata gaaattccgt ttgatgcaat gcgccgccgc tcagagcggc
     1021 gatgaggccc gccgcgataa aggttgcaga ggtaagtgcc tccgccgcaa atagtctgcg
     1081 cgtcgcgcgc aatagccaga tgcttaacgg cgcactcagc aaaataagga tcatggtgtc
     1141 ggaaagagcc ggaacgccat cttctgcaac ccaaaggaat gccaggatgc cgctaccgag
     1201 tgcggtcaat gcttggcgcc ggacaaaact tgtctcgtcc gccctctcga aagggaaaga
     1261 aaacacggcc tgcaaggacg cgttggcggt ccaccgttgc agtttcgaca agaattccac
     1321 agcaaccctc cttgcgcccg cgcgaaatcc ggcaaaggac acttcgttcg cacaaagact
     1381 gccagacggt tctttaacaa ttccctctaa gtttaggatt gccaggccgc ggcgatgagc
     1441 aaaccggtgg aaaattgctc tatggtatag aagccgttgc caggccatgg cgcaaaatta
     1501 agtcaaccct aaccaaatat gtttgactct tctgcaccgt ccgcggggtc ttaagatgct
     1561 gtttctcatc cgcgccattt tttggctctc gatcgtctct tactttctgc cttggccgga
     1621 agacaagatt gctttgttcc tcactaaatc cgacctcaaa agcgaggcgc aagatatttt
     1681 gggccgcaca gttggcatgg cgcaagcggc ggcggaacga gaatgtttgc gcgtgcccgc
     1741 agcttgtctc aaggaagctg ctcggctgag ccgcatggcc gcggggcact tgatggccgg
     1801 caaggataag gcgcctagtc taccgcctgt tgatgcaccc tcggccaggc attaagtaaa
     1861 gagcatcgac cggacctttt gggatcaggc aacggcaaga ggttttatga caggcgctgg
     1921 gatcgccaca aagctcgaag aaatcatcga gaatttcgaa tatttggacg agtgggagga
     1981 ccgctaccgc tacctcatcg agcttggccg cggtctcgaa cctttggacg cggctgccta
     2041 ttgcgacaaa aacaaagtgc gcggatgcgc aagccaagtc tggcttgaga ccaaggtcgc
     2101 gagcgagcag caggggagcc ccgtccttta tttgaagggg gacagcgatg cgcacatcgt
     2161 gcgcggactc gttgctcttt tgcttgcgct ttattccggc caaccggcgg cgcggattct
     2221 tacgatcgat gcccacggct tattcgagaa gctcggtctt ttggctcatt tgacgccaca
     2281 gcgttcgaac ggcgtgcggt cgatggtcga gcggatcaaa agcgaagcgc gcaccgccgc
     2341 cgcggcttag tcgcggcagg gcgaggtctc cgggcagcaa gcaagttttt cgtccaactg
     2401 acccgggtat ccagggctga tcctgcgccg cggcggtctg atcgagcagc cagtttttcc
     2461 aataggcccc gtaattgctg aataaatatc gataataata ttaaacagcc attcgaggag
     2521 tcacgcaaat attgcgtcgc cccagaccta acgtaatgta agcgacagtc agcgaaaact
     2581 ttacgatttc agcattgtca acccccgaat cgaggatgat gggcaaccgg aattgatgga
     2641 aaatcgcttt gtcgcatcaa gacaattctt gcggcaatcc agcaaaacct cagaaacgca
     2701 ttacggggag attcgctatg cttaagttca aaagccgcct gaccgccgcc gcagtcctgg
     2761 ccctcacgtg cctggccaac ggtccggcct gggcagccac gtccgtcact tatgtctctg
     2821 gcaagggcac agatgccggc acttgcgcgg cacctacaaa tccttgccgg acgatccaat
     2881 tcgcagttaa tcaaacagcc ggcggcgagg tcaaggcgct cgaccccgcc gattatagtc
     2941 ccgtgaccat caacaaatcg atcagcatta cggggggcgt atatttcgcg caaaagcgct
     3001 gcgttttcag gatcgcattc ggtcttaaaa agagcctcga tgtcagctgc ttcaagctgg
     3061 tcagcgtcat caagcagttg cacagagttg aaccggacac catcatcaaa atgttctcgt
     3121 gccaggaccc taaggacgct cccagcggct tcaggaaaga gcgattggcc gcaggagaga
     3181 atggcaagcc actcttcata tggcagatcg ttatcaagga atggagccgc atcgccattc
     3241 cgcaaggctt ccacgagatg gccggaaatc tctctaagcc ctcggtccac agcgaatttt
     3301 tcttcgacca tcggaatcca gaggaggcct tgggcttcct ttagcgtgat cgttaaatgc
     3361 gcaattcctt cggtgccgag gtggttgctg gctgcacgag agaagcctga agtcgagatc
     3421 atcacggccg gaatgcctcc cacatcctgc acaagcgcga tggttgatcc ggctattccg
     3481 gcatcgattg gacgcttgtg ccgcttcgcc tctactataa g
//
LOCUS       DATMPU010000283         2397 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_2690266,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000283 DATMPU010000000
VERSION     DATMPU010000283.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 2397)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2397)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2397
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2690266"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1276)
                     /locus_tag="VNY06_06455"
     CDS             complement(<1..1276)
                     /locus_tag="VNY06_06455"
                     /inference="COORDINATES: protein motif:HMM:NF021523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2252 domain-containing protein"
                     /protein_id="HWX14483.1"
                     /translation="MAHATGNGVAGRHIGRAERYAAGRAMRDRVPREALADCPLSAGR
                     DPVAILHETDAERIPEILAERYKRMMKSPFAFLRGAAAVMAQDLAGQPMAGIPVQACG
                     DCHLMNFGAFNTPEGRVLFDINDFDETLPQVDFTVDLKRLAASVAVAAIDAHMPDKKA
                     RALAEVTVKSYREFLLELATQPPLEIWYTRMDVAREVKKISDDKLREKIFSTLVKAKK
                     DLAADDNFPHLATTNGDTARIEDRQPLICHFDTEALKPYKIHAHSAFANYRGTLLPER
                     RKLAEHYDLIDLALKVVGVGSVGTFCAIGLFMTSDGEHLFLQVKQALHSVLEKLAAPP
                     LDLTHQGARVVEGQRAMQAASDIFLGWTEDPKTGRYFYVRQLKNRLLGSIGEVMESGG
                     LDAYASLCGRTLARAHARTGDSVLLAGYMGKSE"
     gene            complement(1635..>2397)
                     /locus_tag="VNY06_06460"
     CDS             complement(1635..>2397)
                     /locus_tag="VNY06_06460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012047207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HWX14484.1"
                     /translation="NFTDAGAVASGIGRYAGRAGGIMFAIALIDASIIGASAVSLATA
                     YAIGDVFSLRHSLHRKPSDTKGFYFVYCGLIVIAAGLVLTPGAPLGPLTNAVQTLAGV
                     LLPSATVFLLLLSNDKAVLGPWVNSAWLNLATGSIIAVLVMLSVILTVAVLFPEAANE
                     TVILSILGGGTILGVFIAVGTLLLRGKQRKMEVAAAKHAPISRDQWHMPPLHKLPPAH
                     LSLAARSWMLVLRAYLVLAAGLILYRIVPFSIGRA"
BASE COUNT          525 a          668 c          739 g          465 t
ORIGIN      
        1 cttctgattt tcccatatag cccgcgagaa ggacagagtc gccggtgcgt gcgtgcgccc
       61 tagcaagtgt cctgccgcaa aggctcgcat aggcatcgag ccctccgctt tccatgactt
      121 cgccgatgga tccaaggagc cggttcttga gctgacgaac atagaaatac cggccggtct
      181 ttgggtcctc tgtccagccg agaaaaatat cgcttgctgc ctgcatcgcg cgctggcctt
      241 cgacgacacg ggcgccttga tgggtaagat caagcggagg cgctgcgagt ttttcgagaa
      301 ccgaatggag cgcctgcttg acttgcaaaa acaaatgttc gccatccgag gtcatgaaaa
      361 ggccgatggc gcaaaatgtg ccgacgctgc caacgccgac aacttttagg gcaaggtcga
      421 tgagatcata gtgctccgcg agtttgcggc gttcgggcag aagcgtgccg cggtaattgg
      481 cgaaagcgga atgggcgtga attttgtagg gcttcaatgc ttcggtatcg aaatgacaaa
      541 tcaaaggctg ccggtcctcg atccgggcgg tgtccccatt tgtcgttgca agatggggga
      601 agttatcgtc tgctgccaaa tctttcttcg ctttaacgag ggtggagaaa atcttttcgc
      661 gcagcttgtc gtcgctgatc tttttgactt cacgagcaac gtccatccgc gtgtaccaaa
      721 tttcaagcgg cggttgtgtc gcgagttcga ggaggaattc gcgataggat tttactgtca
      781 cttcggcaag cgcgcgggcc ttcttatcgg gcatatgggc atcgattgcg gcaaccgcga
      841 cgctcgccgc caggcgcttg agatccaccg tgaagtcgac ttgaggcaat gtttcatcga
      901 aatcgttgat atcgaaaagg actcgtcctt ccggtgtgtt gaacgcgccg aaattcatca
      961 aatggcagtc cccgcaggcc tgcaccggga tgccagccat aggctggcca gcaagatcct
     1021 gagccatgac ggcggcagcc ccgcgcaaaa aggcgaaggg cgatttcatc atacgcttgt
     1081 agcgctcagc tagaatttcg gggatacgct cagcgtcggt ctcatggaga atggcaacgg
     1141 ggtcccggcc ggctgacaaa gggcaatcgg cgagggcttc gcgtggcacg cggtcgcgca
     1201 tcgcgcggcc ggctgcgtag cgttccgccc gcccaatgtg tcttccggca acgccatttc
     1261 cagttgcatg tgccatattg cctcctgaac tgtcccacag ggaacataac cgcgtatctg
     1321 acgcgcgaag gcgacgaaaa tcaatggcag agttgtctgt ggccttccgg ccttgccgcc
     1381 acgagcggat ggcccgcatt ctgccacctt ttggcgccct cccggtacct gccgacagtg
     1441 cgatagccat aggtgagcgc ggcttcgccg cctagcgcgc cgcgcgacca atgcgcgggg
     1501 ctaacgcggg tcagagatgg gtcgactatt cttgcctacg atgcggcagc ctggaaggac
     1561 gcagggcgaa acattttccg cgacggtgga aacgggctgc ggctggggcc agccgagaat
     1621 aaattgtttc aagcctaagc ccggccaatt gaaaaaggca cgatccgata caaaatgagc
     1681 ccggccgcga gcacgagata ggcgcgcaag accagcatcc agctccgtgc tgcaagagac
     1741 aaatgcgccg ggggaagttt gtgcaaaggc ggcatgtgcc actgatcgcg ggagatgggt
     1801 gcgtgcttgg ctgcggcaac ctccatttta cgctgctttc ctctcaaaag cagagttccc
     1861 actgcgatga aaacgccaag gatcgtgccg cctccgagaa tagagaggat cactgtttcg
     1921 ttcgccgcct cagggaatag gaccgcgacc gtaagaatga ccgacagcat gacaagaaca
     1981 gcgattatgc tgccggtggc caaattgagc caagcggaat tgacccaggg accgagcacc
     2041 gccttgtcat tgctgagcag caggaggaaa accgtggcgc tcggcaaaag cacaccggca
     2101 agggtctgca cggcatttgt cagagggccg agcggtgctc cgggcgtgag gacgaggccg
     2161 gcagctatca cgatcaggcc gcaatagaca aaatagaagc ccttggtgtc ggagggcttc
     2221 cggtgcaggg aatggcggag cgagaaaacg tcgccgatgg cataggctgt cgcgagcgaa
     2281 acggccgagg caccgatgat gctggcatcg atgagggcaa tcgcgaacat gatgccgccc
     2341 gcccggccag cgtatctgcc gatcccgctt gctacggcac cggcatccgt gaaattc
//
LOCUS       DATMPU010000284         2145 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_3025226,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000284 DATMPU010000000
VERSION     DATMPU010000284.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 2145)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2145)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2145
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3025226"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..914)
                     /locus_tag="VNY06_06465"
                     /pseudo
     CDS             complement(1..914)
                     /locus_tag="VNY06_06465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_043766018.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            complement(966..1908)
                     /locus_tag="VNY06_06470"
     CDS             complement(join(966..1571,1573..1908))
                     /locus_tag="VNY06_06470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611132.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HWX14485.1"
                     /translation="MARPYSMDLRERAVARVQAGESVRVIARALSISPSSAVKWAQRF
                     RATGSAAAGKMGGHRPRLVVGEHADFLRERIAQGGFTLRGLVAELAARGLKVDYRTVW
                     TFVRGEGLSFKKTVLPSEQDRPDIARKRARWKAYQGRIDPRRLVFIDETWAKTNMAPL
                     RGWGLRGKRLKAKAPYGHWKTMTFLAALRHDRIDAPWVVDGPINGELFRLYVEKVLVP
                     MLKRGDIVILDNLGSHKSKAVRAAIRAAGARLFFLPPYSPDLNPIEQVFAKLKHLLRK
                     AAERTVEATWKRIGALLDCFLPNECQAYLANAGYGSA"
BASE COUNT          377 a          649 c          660 g          459 t
ORIGIN      
        1 gatgaatgat tggatggttt ggcgtaggcc cattcccgca aggaggtcgg cacgaagcgt
       61 tcggcctttc cgtttgttct tggcgtatag ggtctggtgc gcttgggctt gatttttagc
      121 attcgcaacg cctttgtgta gcggcgtgta gcggcgcgaa cggaagctga cgccattgtc
      181 gccattgtcg gtcatgacgc gaaggaccct cacgccgtga tcgcgataga agcgcaaggc
      241 attgaacaga aatttcaagc aggagcggcg tgtttcatct ggcaggattt cagagcagga
      301 tttcagagca ggccagccgg gagtgatcgt cgacggccag atccaaatat tcccagccct
      361 tgccgccttg cttgcggctg cgcggcgcgc tttgcccggt gcgattgccg gtgatgcgat
      421 gtccgacccc ttcgatgcgg ccgagcttct tgatgtcgat gtggatcatc tcgccgggca
      481 aggtcttctc atcgcggacg atctcaattt tcggatcgag gttggagagg cggctcaacc
      541 ccttggcctt gccgatgcgg gccacggtcg cgcgcgacag cccggtctcg cgggcgattt
      601 tccagaacgg ttgacggttc cggcgcaggg cttcgaccgg catgatctgc tcggatgacc
      661 cgcgtgcgca ggcgatgcgg ccggctggtc cggtcctcga gacccgctac gccttcgctt
      721 tcgtggcgcc ggagccattt cctgacggtc gccggcgaaa cacctacggc gccggctatg
      781 gcgcgcggcg tttcgcccgc tcggagcgtt gcgacaatca ggactcgcat gttcggcgtc
      841 gtcctggcat tcttgtgaat gtcacgggtg ggtctcctga gcttgtggcg cttcgcaaac
      901 tccccaaacc tcagtttctc cccgggaaca acctatcgag acagaacagc tagagcgtga
      961 tgtgtttaag cagaaccgta tcctgcgttg gcgaggtagg cttggcattc gtttggtaag
     1021 aaacagtcga gcagagcgcc gatgcgcttc caggtcgcct caactgtccg ctcggcggcc
     1081 ttgcgcagga ggtgtttgag cttggcgaag acttgctcga tcgggttgag atcggggcta
     1141 tagggcggga ggaaaaagag ccttgcaccg gcggcccgga tcgcggcgcg gacagccttg
     1201 cttttgtggc tgcctagatt gtcgagaatg acgatgtcgc ctctcttcag catggggacg
     1261 aggaccttct cgacataaag acggaacagc tcgccgttga tcggaccatc gacgacccat
     1321 ggcgcgtcga tccggtcatg ccgcaaggcg gcaaggaagg tcatcgtctt ccagtggccg
     1381 tagggggctt tggccttgag cctctttccg cgcaagcccc atccgcgcag cggcgccata
     1441 ttggtcttgg cccaggtctc atcgatgaag acgagccgcc gcggatcgat ccgcccctga
     1501 taggccttcc agcgcgcccg cttgcgggcg atgtccggcc ggtcttgctc gcttggcaga
     1561 acggtttttt ttgaagctca ggccttcgcc acggacgaaa gtccacacgg ttcgatagtc
     1621 caccttgagg ccgcgggcgg cgagctcggc gacaagcccc cgcaaggtga agcccccttg
     1681 cgcgatccgt tcccgaagaa agtcagcgtg ctcgccgacc acaagccgcg gccggtggcc
     1741 gcccatcttc cctgccgcag cgcttcccgt tgcccggaac cgctgcgccc atttcaccgc
     1801 gctcgaaggg ctgatgctca gcgcccgagc aatgacacgc acgctctcgc ccgcctgaac
     1861 ccgcgcaaca gcccgctcgc gcaaatccat cgaatagggt cttgccatct gccaaaagcc
     1921 tccgactcaa tccaatcctt gaatcagaaa ctcggctagt tgggaatccc ctgccgattc
     1981 agccaagcct catcccgctc taacagtctg cggaaaaacc gggaaatttg agcttttgcc
     2041 gttccggtat ggagctttgt gctggtggtt cagtcgtttg gctcggcata aaaggctctc
     2101 tcggtttctt tacggagttc gcctatgcgg caaccaattt cggga
//
LOCUS       DATMPU010000285         3795 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_1470104,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000285 DATMPU010000000
VERSION     DATMPU010000285.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 3795)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3795)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3795
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1470104"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..196
                     /locus_tag="VNY06_06475"
     CDS             <1..196
                     /locus_tag="VNY06_06475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007800392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX14486.1"
                     /translation="GALLSRPAAYRAAIAAADIALAHLPRFVIYNGLNAWGKHREVPH
                     PPETTTQHLLAFGREPAISI"
     gene            complement(262..426)
                     /locus_tag="VNY06_06480"
     CDS             complement(262..426)
                     /locus_tag="VNY06_06480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012029389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3309 family protein"
                     /protein_id="HWX14487.1"
                     /translation="MSLGTILLIILIIALLGGFSGLGGGPFYGTGYYGGGGLGLVLVV
                     VLILVLLGKL"
     gene            complement(1169..1435)
                     /locus_tag="VNY06_06485"
     CDS             complement(1169..1435)
                     /locus_tag="VNY06_06485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HWX14488.1"
                     /translation="MPPPMRLSRDLLVRGIIYKLQERAYADALTKAGEIEERLGNDRG
                     QAMVLNSLGGVLQRLGKFPEAADALERSTRNVSRSTMRQGLGAC"
     gene            1439..1930
                     /locus_tag="VNY06_06490"
     CDS             1439..1930
                     /locus_tag="VNY06_06490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX14489.1"
                     /translation="MKPPPLAAQLEDRAESRASALAISIAALSMSARTVPPPEAVASP
                     DPTDVLLVYQPAPLTSDWVHEFRSLLQEWLSESLGRQTTITDLAETEAANGKLSISGN
                     LPESQIAVVIVTRRLLKSVWAEALIPDVVAIYGRGRLLCVALDRGAASESAGQATRAL
                     LLQ"
     gene            2261..2914
                     /locus_tag="VNY06_06495"
     CDS             2261..2914
                     /locus_tag="VNY06_06495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010683372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HWX14490.1"
                     /translation="MTASLGSAEAKEPSAGQPASNAIGADAALSLLMKGNVRYVANRP
                     THRDFSAGRAERTKSQYPIAAVLSCSDSRVAPEFAFDQGPGDLFVVRVAGNFANEDGL
                     ASFEYAVKFLGVPLVVVLGHSNCGAVDAAIKVIEENASLPGHLPGLVQSIKPAVEAAK
                     LKAPEDLLTAAITENVALNVKRLQTAQPLLASFAQNKRVKVVGGVYDLATGKIGLLS"
     gene            complement(3455..>3795)
                     /locus_tag="VNY06_06500"
     CDS             complement(3455..>3795)
                     /locus_tag="VNY06_06500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX14491.1"
                     /translation="LQKLGRSLHLLLPPFPFIKKGDAGMVDGAADAARADGQNAWNAW
                     RRDPANKDVHVIFAGIDFSEAPRDRIDFSGFEFGDLADFSRTETLHHILSVMAQHSGA
                     KMPKLGNSNN"
BASE COUNT          799 a         1075 c         1139 g          782 t
ORIGIN      
        1 cggcgcgctg ctgtcgcggc ctgctgccta tcgcgcggcg atcgctgccg ccgatatcgc
       61 gctcgcgcat ctgccgcggt tcgtcatcta caacggcctg aacgcctggg ggaagcaccg
      121 cgaggtgccg cacccgcccg agacgacaac gcagcattta ctagctttcg ggcgggagcc
      181 agctatctca atctaattgc ccagccggcc gagcggcatt ggtcctggtg gggcagcaaa
      241 aagaccacaa ccgctggatg ttcaaagctt gccaagcaag actaagatca acacgacaac
      301 caacacgagg ccgaggccgc cgccgccgta gtagcctgtg ccatagaaag ggcctccacc
      361 taagccgcta aaacccccaa gtaacgcgat gattaaaatg atgagcagga tggttccaag
      421 tgacacggca ttatctccta gtttgactga gccagcgccg cgttgctatt caccccgatt
      481 ttgggtgcct gtcaacggcg gagcaaaagt cggccagtgg gggcttcgtt gcacggatgc
      541 tcgtgagtga tgattttccc gtgcaacgaa tagggtccgc gtgagaggtg cgtcctgatg
      601 acgggaagat cagcagtttt tggcttacct tccttgccac acttcgcgag gatgacaaga
      661 agccaagcca ggcgcttctc gataggggtg ggccctctgg atgtgtcctg tgcgagcaat
      721 ctgcttgatg tcgaatggtt gagcgggaaa gggaaagtgg ttcgcttgac ggaataatgg
      781 ggatatcgcg atgccgattg ttgcgcggcg tgaagttgca tggcatttct gttgggtggt
      841 cacatgcgga aaggaggtgc gtctatgaag ctaattacat ggcttcttac gcatcgccac
      901 ccgaaaaccc catggcgggg ctaaccttgg cctacgttcc gccgttggta gatcggcggg
      961 aaagtcaccc tattcagggc gatgggcagt agcggcgaca gccgccaagg tatcgcggca
     1021 aggcatgtct cgaggggcgg cgctttctac ccggcgcgcc cctcacaatt agattacgtt
     1081 tttgagcctc ctcccacaca gatgaatgac cagacctcgc atcgtaatga tgcgaggtct
     1141 gcttagtggt gtggcaaagg agcaaggatc aacaagcccc gagcccctgt ctcattgttg
     1201 atcgggagac attgcgtgtg gagcgctcca gcgcgtctgc ggcctccgga aacttgccca
     1261 agcgctgcag cacgccgccc aggctgttga gcaccatcgc ctgcccgcgg tcattgccaa
     1321 gccgctcctc gatctcgccc gccttggtca gcgcgtctgc ataggcccgc tcttgaagct
     1381 tataaatgat tcctcggacc aagaggtcgc gggagagccg catcggcggc ggcattcggt
     1441 gaagccgccg ccactcgccg cgcagctcga agatcgtgcc gagagccgcg cgagcgccct
     1501 tgcgatatcg atcgccgctt tgtccatgtc agcgagaacc gtcccgccgc cggaagccgt
     1561 ggcgtcaccc gacccgacgg atgtgttgct ggtgtaccag ccggcgccgc tgaccagtga
     1621 ctgggttcac gaattccgct cgctgctgca ggaatggctg agcgagtccc tgggacgtca
     1681 gaccaccatc accgacctcg ccgagaccga agcggcgaac ggcaagctgt cgatcagcgg
     1741 gaacctgccc gaatcccaga tcgctgtcgt catcgtcacg cggcgcctgc tcaagtccgt
     1801 ttgggccgag gccctgatcc ctgacgtagt cgcgatctac ggccgcggcc ggctcctctg
     1861 tgttgccctc gaccgcggcg cggcgagcga gtccgctggc caagcaactc gcgctcttct
     1921 tcttcaataa agcggaattg cgcgcgatgg cgcgcgcgac ggccgaaaac ggcagaggtt
     1981 ttcagcggcg cctcaacgcg cactatcgag gccattacgc cttacctcac gcaagtgatg
     2041 gttgcgccgg aaactgatgg ctggtgaggg tcaaggcaag caagccgaaa ttcgatgccg
     2101 cggtcgggcc tgcgatcaag tccaccttca ttcttgtcat gcggtcacgt tgagataaaa
     2161 aaatgctgcg aaactaggag cgccaaacat gtgtctcacg ttccattgtc ctcattcttt
     2221 gtcgcggcgg gatgcattca aagccgctgc aggatttgca gtgacggcga gtttgggctc
     2281 cgcggaagca aaagagccga gcgctggtca gccggcgtca aacgccatcg gcgcggatgc
     2341 cgcgctcagc ctcttgatga aaggcaatgt ccgctatgtt gccaatcggc cgacacatag
     2401 ggatttttct gccgggcgcg cggagcggac caaatcgcaa tatccgatcg cggcagtgct
     2461 cagctgctcg gactcgcgcg tggccccgga atttgccttt gatcaggggc ccggtgatct
     2521 ctttgtcgtt cgtgttgcgg gaaatttcgc caatgaggat ggccttgcga gtttcgaata
     2581 tgcagtgaaa tttttgggcg tgccgctcgt tgtcgtgctt ggccattcga attgcggggc
     2641 ggtcgatgcc gcgatcaaag tgatcgagga aaacgcaagt ctgccaggac atttgccggg
     2701 tctcgtacaa tcgatcaaac ctgcggtgga ggcggcaaaa ttgaaggcgc cggaggatct
     2761 tctcaccgcc gcgatcacgg agaatgttgc gctcaacgtc aaacggcttc aaacggcgca
     2821 accattgctc gcgagctttg ctcaaaacaa aagggtcaag gtcgtgggcg gtgtttatga
     2881 tttggcgacc ggcaagattg gcctcttgtc ttaaatttgt gcccgcgcaa acttatcgcg
     2941 ggcaaccaat ccggataaag gcggatatca tagtgttgaa caattggtgt gcctgcatga
     3001 aacactccgg gcggcgccat gtcgtggcat ggggagaggc ctggcaaacg aaagcgcctc
     3061 ctccgcaaaa gaaagcccca gcgctgcgcc atctatacgc gcaaatcgac agtcggcgag
     3121 tcgaatttgg aggtcgggcc cgagcgaact ccggccccct agaaccagag cctgatgctc
     3181 gttggacagg gcgccgcaga agagcatctc agaaatcctg tcggctggat cgctcgctgt
     3241 aaattactgc cgccattacg agcatccatc cgtaaagtgg gacccgtgtt gccaagtgaa
     3301 aagaactaaa aagtggtcgg gcaacgggaa cttaactaga agttaggcgt tataggggca
     3361 agggcgatgc cagaaaacgt ggagcgggga tatgtctggg actctatatg caactctgta
     3421 tgtttcagtg atagcgatcc tgtttatcat tgcctcaatt attggagttg cccagttttg
     3481 gcatttttgc gccagaatgt tgagccatta cggagagtat gtgatggagc gtttcggtgc
     3541 gcgaaaaatc cgcaaggtcg ccgaattcga accctgagaa atcgattctg tccctcggcg
     3601 cttcggaaaa gtcgatgccg gcgaaaatca cgtgcacgtc cttgttggcc ggatcgcgcc
     3661 gccatgcgtt ccaggcgttt tgcccatctg ctcttgccgc atctgccgct ccgtccacca
     3721 tcccagcgtc gccctttttt ataaacggaa acggcggaag cagaaggtgc agagaacggc
     3781 ccaatttttg taaaa
//
LOCUS       DATMPU010000286         3513 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_3936693,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000286 DATMPU010000000
VERSION     DATMPU010000286.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 3513)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3513)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3513
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3936693"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..832)
                     /locus_tag="VNY06_06505"
     CDS             complement(<1..832)
                     /locus_tag="VNY06_06505"
                     /inference="COORDINATES: protein motif:HMM:NF017853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF937 domain-containing protein"
                     /protein_id="HWX14492.1"
                     /translation="MFNLYEILRSAQGGQAVENLASQFHVTPEEADAAVKAIVPELSD
                     GFLKQASKPVAFGSFFGSLGDGHYRAAFADPAAAQASAQSSGDFLTQVLGSSSAQEEI
                     VQRAASATGIAQDTLSKMLPVIVSTILGGLTKSLENQGFGGILGQLSNAASQGSLGPV
                     LGQILGGGPAAASSPQPAQVPGPLAGVGGLGGILGAILESLKGQAGASRNAGPATSGA
                     APEQTPSFDAATIQAGLEALKTILQPGTPPASHSQAPAPRTGTPGGQQTDISAELDRI
                     I"
     gene            complement(900..2252)
                     /locus_tag="VNY06_06510"
     CDS             complement(900..2252)
                     /locus_tag="VNY06_06510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020176784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HWX14493.1"
                     /translation="MTSGLRPRAVFTEGSTMRHVIVMTAATSVGLMAIFVVDLLSLLW
                     VSRLGDPKLTAAVGFASQVLFFSISINIGFSIAIGALVSRALGAGDRKAARRLAGSGL
                     SHVFVVAALVTFCAWPLRREILSILGASGITLDVASAYLAITLPATALLGLAMGLASI
                     LRAAGDARRSMYVTLSGAIATAVLDPLFIFGLGLGVTGAAIVTMIARIIAVAVGLNGA
                     MRKFDLVALPSVETARVDLKAMMSIAILAVLTNLATPVASAYTMRVFSGFGEAVVAAF
                     AIVERLTPMAFGVLFALSAAVGPIMGQNLGANLIARVRQVLTDCLILAATYVVVVSIV
                     LGFAAPFIVKLFGAQGETARLVVFVCTYGGVLWLFLGAIFVANAAFNNLGSPLFSTVF
                     NWGRATLGTIPFVTLGAAHFGPEGGYVGLIAGSTLFGAAAVLAAYHVTARLAKGVKVL
                     "
     gene            complement(2302..2928)
                     /locus_tag="VNY06_06515"
     CDS             complement(2302..2928)
                     /locus_tag="VNY06_06515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012386571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-type transport auxiliary lipoprotein family
                     protein"
                     /protein_id="HWX14494.1"
                     /translation="MRNLGAFRVSVFGNFRAAPGGKIFIILIAGLPAVLLGACSSAPK
                     LTYDLNPIAGDFAARGGRGQLAIAVPDATLPANSDEIVVRTGPQSVAYLSGAQWADKL
                     PSLVQSRLIESFQNAHLLRAVGRPGILADATLHTTLRHFDFDAGQGEAFVQISVQLIA
                     AGGRIIAGRLFSAKVAAPSGEPSAVAAALDAALRQVMREIVLWTAPKV"
     gene            complement(3169..>3513)
                     /locus_tag="VNY06_06520"
     CDS             complement(3169..>3513)
                     /locus_tag="VNY06_06520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012591792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MCE family protein"
                     /protein_id="HWX14495.1"
                     /translation="IVSEFQVLSQKLEKAANSLSSFLETGESKGPLGEVAAAAKSIRK
                     AADGFNAQIRTIGENINRFAGSGLRQYEALAVDGRKTLEEINQAIHSLESNPQQLLFG
                     KSPQIPQYSGGH"
BASE COUNT          815 a         1069 c         1005 g          624 t
ORIGIN      
        1 cgatgatccg gtcgagttcg gcgcttatgt ctgtttgctg ccctccgggt gttcccgtcc
       61 ttggggcggg agcctgcgaa tgtgaagccg gcggtgttcc gggctgcagt atcgtcttca
      121 gcgcctcaag acccgcctgg atggtcgcgg catcgaaact gggcgtctgc tccggagcgg
      181 caccagaagt ggcgggtccc gcattacgag aagcacctgc ttgaccttta agactttcca
      241 aaatggcgcc taagataccg ccaagccccc cgaccccagc caacggacca ggcacttggg
      301 ccggttgcgg cgagcttgct gccgccggtc cgccccccag aatttggccg agaacggggc
      361 caaggcttcc ttggctggca gcgttcgaaa gctgtcccaa aattccgcca aaaccctgat
      421 tctccaatga cttcgtgagg cccccaagga tcgtcgagac aatcaccgga agcattttcg
      481 ataaagtatc ttgggcgatg cccgtggcgc ttgccgcacg ttggacgatc tcctcctgcg
      541 cgctgctgga gcctaggact tgggtcaaga aatcgccgct actttgcgcg cttgcttgcg
      601 ccgcagctgg atcggcaaag gccgcgcgat aatggccgtc cccgagggag ccgaaaaagg
      661 aaccgaacgc gaccggtttg gacgcctgtt taaggaaacc atcggaaagc tccggcacaa
      721 tggccttgac cgccgcatcc gcctcctcag gtgttacatg gaactgagac gccagatttt
      781 cgaccgcttg gcctccttgc gcactgcgca agatctcgta aagattgaac atgtccgtcc
      841 tcccttgttt gcccgcgacg cagactgcct tcaggcgtcc ggcaggaaac caaggaatgc
      901 tacaagacct taacgccttt tgcgagccga gccgtcacgt gataggcggc aagcaccgcc
      961 gccgctccaa acaaagttga acctgcaatc agccccacat aaccgccctc gggtccgaaa
     1021 tgcgcggctc cgagcgtaac aaatggaatc gtgcctagcg tggcgcggcc ccaattgaaa
     1081 actgtcgaga acagcggaga ccccagattg ttgaaggcgg cattggcaac gaaaatagcg
     1141 ccgagaaata gccaaagcac tccaccatag gtgcaaacaa aaacaacgag cctcgctgtt
     1201 tcaccctgtg cgccgaagag tttgacgatg aaaggcgcgg caaacccaag aacaatcgag
     1261 accacgacga cataagtcgc agccaggatc aggcaatcgg tgagcacctg acgcacccga
     1321 gcgattaaat ttgccccaag attttggccc atgatcgggc cgacggccgc ggacaatgcg
     1381 aatagaacgc caaaggccat cggggtcagc cgttcgacaa tcgcaaaagc agccacaacc
     1441 gcttcgccaa agcccgaaaa aacccgcatg gtataggcac ttgcaactgg tgtcgcgaga
     1501 ttggtcagga ccgcgagaat agcaatcgac atcatagctt tgaggtccac cctcgcggtc
     1561 tcaacgctcg gaagcgcaac aagatcgaat ttgcgcatgg cgccatttag gccgacggcc
     1621 accgcaatga tgcgtgcaat catcgtcaca atggctgcgc ccgtcacacc aaggccaaga
     1681 ccaaaaataa acaaaggatc gagaaccgcg gttgcgatgg cgcccgagag cgtcacatac
     1741 atcgagcgcc tcgcgtcgcc agccgcgcgc aaaatgctcg caaggcccat cgcgaggccg
     1801 agcagagccg tcgccggcag cgtaatcgcg aggtaagccg aggcaacatc gagagtgatg
     1861 ccactggcac caaggatact aagaatctcg cgccggagcg gccaagcgca aaaggtgacc
     1921 aaagccgcga ccacaaaaac gtgggaaaga ccggagcccg cgagcctccg agcggccttt
     1981 ctatctcccg cacccagcgc acgcgaaaca agagcgccaa tggcaatcga aaagccgatg
     2041 tttatggaga tcgaaaagaa caaaacctga gacgcaaagc cgacggcagc ggtcagtttc
     2101 gggtcaccca gcctcgatac ccagagcaga gacaacaggt cgacgacaaa tatcgccatg
     2161 agcccgacgg aggtcgcggc ggtcatgaca atcacatggc gcatcgtcga gccttcggtg
     2221 aagaccgcac gcggtcttag tccagaggtc aacggggaga ttccttcttc gctgctcgtc
     2281 tgccagattg cggggcgcct gtcatacctt tggcgcggtc cacaggacga tctcgcgcat
     2341 cacttggcga agcgccgcgt caagggctgc ggcaaccgca gaaggttcgc ccgacggcgc
     2401 cgcaactttg gcagaaaaca accggcctgc gattatacgt ccgccggccg caattagttg
     2461 caccgagatt tggacgaaag cttcgccctg accggcatcg aaatcgaagt ggcgcagcgt
     2521 cgtatgcagc gttgcgtccg cgaggatccc ggggcggccg acggcgcgca gaagatgcgc
     2581 gttctgaaag ctttcgatga gccggctttg aacgagcgac ggcagtttgt ccgcccattg
     2641 cgcgccactg aggtaggcga cggattgcgg accggtgcga accacaatct catccgaatt
     2701 ggctgggagc gttgcgtctg gcacggcaat ggcgagctga cctcggccgc cgcgcgcggc
     2761 aaaatcgccg gcaatcgggt tcaagtcgta agtcagcttc ggagcggatg agcaggctcc
     2821 gagcaacacg gcgggcagtc cggcaatcaa gatgataaaa atcttgcccc ccggcgcggc
     2881 gcggaaattg ccaaagaccg agacgcggaa ggcgcccaag ttcctcattc gaaaatccat
     2941 tcccgcaatt ttggggctct tggccatcgt ggcagcccca acttccgcta attgcagcgg
     3001 ccgattgcgt caaggcaggc gccggtcaat tcccattcca tggcgcgctt ccttgcaaag
     3061 acgccagtga gcgacgggac gactccttaa accgtcctct ccgctcaaat tcttgctctt
     3121 gtcgcctgtc cggacgcaaa accaggtccg ctcttggctc gaaacgctct aatggccacc
     3181 cgaatactgg ggaatttgcg gcgatttacc aaaaaggagc tgctgagggt tgctttcgag
     3241 cgagtggata gcctgattga tctcctcgag cgtcttgcgg ccatcaaccg ccaaagcctc
     3301 atattgtcgc aatccactac ccgcaaagcg gttaatattc tcgccgatgg ttctgatttg
     3361 ggcattgaaa ccgtcggcag ccttcctgat cgatttggcg gcggcagcga cctcaccgag
     3421 cggacctttg gattctcctg tttcgaggaa actgctcagg ctattcgcgg ccttttcaag
     3481 tttttgcgag agcacctgga actcggagac gat
//
LOCUS       DATMPU010000287         4485 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_1470884,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000287 DATMPU010000000
VERSION     DATMPU010000287.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 4485)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4485)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4485
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1470884"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..109)
                     /locus_tag="VNY06_06525"
     CDS             complement(<1..109)
                     /locus_tag="VNY06_06525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012590157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HWX14496.1"
                     /translation="MLQNLTERAPAKINLTLHIVGRRADGFHTLESLVTF"
     gene            complement(394..2184)
                     /locus_tag="VNY06_06530"
     CDS             complement(394..2184)
                     /locus_tag="VNY06_06530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018264405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HWX14497.1"
                     /translation="MPMTWKLAKNDRWPHRYRSAWGLAACLLLALVFPAASAQARVRE
                     NLAIATPFEVGESPAGNYLAASIAVVEHDTVAAATFFREALRYDPRNPSLLERAFVAA
                     VSNGNMQDAFGLADRLLERDPKNSLARLVLGVKAIKAKRFTAARAQFKVGGSGQQHDI
                     TTTLLTAWTYAGAHDKRRALSLVDKLRGDNFAVFKDYHAALIADAANDPQEASKRMKA
                     AYASDKNTLRLVDAYARFAARHNDRAEAVRAYQAFDQLLPNHPIVSAMLADINSGKPI
                     EPLVKNAEEGAAEVLYGLGAAGGQQGDELAAMIYLRLALYLTPQNGLAIISLGDLYER
                     IKQNEQAIDVYGLVQSTDPLRTTADIQTGQILEAMGRPDEATKYLKRVVDENPSSEDA
                     LSALGNLQRAHKQYGEAIETYSRVLAKTTTPQKANWPIYYFRGISYEREKKWPQAQAD
                     LMQALALFPDQPLVLNYLGYSWVDQGMNLDEAFTMLQRAVELRPTDGYIVDSLGWANY
                     RLGRIDESVKQLERAIDLKPSDPVINDHLGDAYWRAGRQLEAHFQWNHARDLGPEPED
                     KDKILRKIEHGLEDEPKPAAADAEPKKSGG"
     gene            complement(2496..3752)
                     /locus_tag="VNY06_06535"
     CDS             complement(2496..3752)
                     /locus_tag="VNY06_06535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003612894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall hydrolase"
                     /protein_id="HWX14498.1"
                     /translation="MPANGRGTIMRCLQGSWALGVVAPWCLGIALAVSITADAGEEVS
                     SGTSIAPLALRTQSPPTELIPAQPSALGIDFGSFTGEAQQILRQASLSIGTSEEFKRL
                     PDEVEPRAELKRNAQHFPEIDRSHRGDPFTGLRPAFDTRLRNFPGLARFRAADLIFHH
                     DDVEPASSFSVSEEALGPESVAAFEPWLEGQSPTTAQSRAEVSPPQAASPETVRPAAL
                     NERLMQGATPAILRAIALGSATPAPADSTPIEIAAVSGNSRGYLVHGEGYARLRPNYA
                     ALIDQDTAMQEKRCLAEAIYFEARGEPKEGQAAVGQAVLNRVSSGLYPPTICGVVYQN
                     RRHYNACQFSFACGGKALRITQPDAWREAQRIAAEVTNGSAYLSDIGSSTHFHANYVR
                     PRWARRLEKMDVIGHHIFYKLKPGQS"
     gene            complement(3987..>4485)
                     /locus_tag="VNY06_06540"
     CDS             complement(3987..>4485)
                     /locus_tag="VNY06_06540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX14499.1"
                     /translation="ASMGSARAQTAAGPEPDYNLAYALATASYCAYGVGQKDADQSHD
                     RAVKCLRLAAKAGSRLNGLDVGPNNVEAYVNPVKPVDAYLLINAKDSFKTFFSGQGHG
                     GAGSVGERDILPPFRRHAFRGFFVRSEARMRQRQDRVGAIFSRPAFMNTRRRSCPAIR
                     NGATC"
BASE COUNT          877 a         1280 c         1345 g          983 t
ORIGIN      
        1 aaaatgtgac gagactttcg agcgtgtgaa acccatcggc gcggcggccg acgatatgca
       61 aggtcagatt gattttcgcg ggtgcgcgtt cggtaagatt ttggagcaag gtgaccaaat
      121 agagtcaggc acagatgaat atcagatcag ggttgggggc gtcggccacc cgacgcgcac
      181 gagtgtttca tcggatcgga gagcgcgaag tgaatgttcc cgcctttacc gtcggataaa
      241 atccggtagt tttcatgcgc agcgtcacgc gtaaaaccga gcttggcgta aaatgcctcg
      301 ctggcatcta ggtcattgca agggagaatg gttgtcaaac catgcgcacg ggacacggcg
      361 gcgtccgctc gttcaaggtg gtgtagcggt gagtcagccc ccgctttttt tcggttcggc
      421 atctgccgcc gccggttttg gctcgtcctc gaggccgtgc tcgatcttgc gcaggatttt
      481 atccttgtcc tctggctccg ggccgagatc acgcgcatga ttccattgga aatgcgcttc
      541 gagctggcgt cctgcccgcc aataggcgtc gccgaggtgg tcgttgatca cgggatctga
      601 gggtttgagg tcgatcgcgc gttcgagttg ttttaccgat tcgtcgattc ggccgagcct
      661 gtaattcgcc cagccgaggc tatcgacgat gtatccgtcg gttgggcgca attcgaccgc
      721 gcgctgcagc atggtgaagg cctcgtcaag gttcattccc tggtcgaccc agctgtagcc
      781 gagatagttc aagaccagag gctggtcggg gaacagtgcg agcgcctgca tgaggtcagc
      841 ttgcgcttgc ggccacttct tctcgcgctc gtaagaaatc ccccggaaat aatatatcgg
      901 ccagttcgcc ttttggggcg tcgttgtctt ggccaagacg cgcgaatatg tttcgattgc
      961 ctcgccatac tgtttatgcg cgcgctgcag attaccgagc gcggagaggg cgtcctcgct
     1021 gcttgggttt tcgtcgacca cccgcttaag atattttgtc gcttcgtccg gccgccccat
     1081 tgcttcgagg atttgaccgg tttggatatc ggctgttgtg cgcagaggat ccgtgctctg
     1141 aaccagtcca taaacgtcga tggcctgttc attttgcttg atgcgctcgt agagatcgcc
     1201 caggctaatg attgcgagtc cgttctgcgg ggtaagatag agagcaagcc gcagatagat
     1261 catggccgca agctcgtcgc cctgctgccc gccggcggca ccaagaccat aaagcacctc
     1321 ggccgcgcct tcctctgcat tcttgaccaa cggttcaatt ggcttgccgg aattgatgtc
     1381 ggcgagcatc gccgagacaa taggatggtt cgggagaagt tgatcgaagg cctgataggc
     1441 acggactgcc tccgcgcgat cgttgtgacg ggccgcgaaa cgggcgtaag cgtcgaccag
     1501 gcgcaacgtg ttcttgtcgg aggcgtaggc ggccttcatg cgctttgatg cttcttgcgg
     1561 gtcattggcc gcgtcggcga taagcgcggc atgataatcc ttgaatacgg cgaagttgtc
     1621 gccccgaagc ttgtcgacaa ggctgagcgc ccggcgctta tcatgcgcgc cagcataggt
     1681 ccaagccgtc agaagagtcg tggtgatatc gtgctgctgg ccgctgcctc ccaccttgaa
     1741 ttgagcgcgc gcagcggtaa accgctttgc ctttattgcc tttacgccaa ggacgaggcg
     1801 cgccaggcta tttttcggat cgcgctccag caggcggtcg gcgaggccaa aggcatcctg
     1861 catgttgcca tttgagaccg ccgcgacgaa ggcgcgctcg agcagacttg gattgcgcgg
     1921 atcatagcgc agcgcttcgc gaaaaaacgt tgccgcggcc acggtgtcgt gctcgacgac
     1981 tgctatggag gccgcgaggt aattgccggc cggactttcc ccaacctcaa atggcgtggc
     2041 tatggccaaa ttttctcgca cccttgcctg cgcgctcgcg gccggaaaaa ccagcgccag
     2101 tagaaggcat gcggcaagcc cccatgcgct gcggtagcgg tgcggccaac ggtcattctt
     2161 ggcaagcttc catgtcatcg gcatcaggcc gtctttccgt gcaagagtcg atcttccagc
     2221 agccaaagct gcgacgatga gatatcacgc ggcgaatatg gcctttttac actgccgcgg
     2281 tgatccgaac ggcttcgcga cagacagggt ggtgcgccaa tcggggaggc gtccctagaa
     2341 caagatgact tatcttcgaa tcgtcatctt gctctaaatc tctgttttgt cgtgtgtccg
     2401 gacgcaaaac cggttaccac ttttgctgga cacggttttg cgcgcgcggc gcgcgcaaaa
     2461 acgccggcaa cgctccacgg cagctagcgc gtcgattagc tctgcccagg ctttagctta
     2521 taaaagatat gatgcccaat gacgtccatt ttttcaagcc ttctcgccca gcgtgggcgg
     2581 acgtaattgg cgtgaaaatg ggtcgagctt ccgatgtcgg acagataggc gctgccattt
     2641 gttacttcgg cggcgatccg ctgggcctcg cgccaggcat ccggttgagt tatccgcagc
     2701 gcttttcccc cgcaggcaaa ggaaaattgg caggcgttgt aatgtcggcg gttttgatag
     2761 acaacgccgc agatcgttgg cgggtaaagc ccacttgaca cgcgattgag cacagcttgc
     2821 ccgacggctg cctggccctc tttgggctct ccgcgcgcct caaaataaat agcttcggcg
     2881 aggcagcgtt tctcctgcat cgccgtgtcc tggtcgatga gcgccgcgta atttggccgc
     2941 aaccttgcgt agccttcgcc atggaccaaa tagccgcgcg aatttccaga aaccgccgcg
     3001 atttcgattg gtgtcgaatc cgccggggct ggcgttgccg atccgagcgc gatcgcgcgg
     3061 aggatcgcgg gcgttgctcc ttgcatcagc cgctcgttga gcgcggctgg cctcactgtt
     3121 tctggagaag ccgcttgcgg tggcgacacc tcagccctgc tttgtgccgt tgtcggactt
     3181 tggccttcta gccaaggctc aaaggccgcc acgctttcgg ggcccaaagc ttcttccgag
     3241 accgaaaagc tgctcgccgg ctcgacatcg tcatgatgga aaattaagtc cgcggcgcga
     3301 aagcgcgcga gaccggggaa attgcgaagc ctcgtgtcaa aagcgggccg cagaccggta
     3361 aagggatctc ctcgatggct ccggtcgatt tccggaaagt gctgggcatt gcgcttcaat
     3421 tcggcgcgcg gctcgacttc atccggcagg cgtttgaatt cttcgctcgt gccaatagaa
     3481 aggcttgcct gacgcagaat ctgttgcgct tcgcccgtga aactaccaaa atcaattccg
     3541 agagcggacg gctgcgccgg gatcaactca gtaggcgggc tttgagtccg caaagccagt
     3601 ggggcgattg acgtgccgga cgaaacttcc tctccggcgt cagcggtaat cgagacagca
     3661 agcgctattc caaggcacca tggcgcaacg acgcccaagg cccaacttcc ttgcaaacac
     3721 ctcatgattg tcccgcgccc gttggcaggc atttttgcgt gcccgattga gcgagtcgct
     3781 gcccgcctgc ccggaaagtt tattgccggg caagcttgag aacttgttac ctgaagcgtt
     3841 agagcagcgt gccagaatgg ggcaggcggg aagttaaggg gctgccggag attaaataag
     3901 cgttcactcg ccaaacagcg ccgcgacgaa ggcgcgcgag cgggctattt caaccgatcg
     3961 cctattttcg gttcaatgct tgggattcag caggtcgcac cattgcgaat agccggacat
     4021 gatctccttc gagtattcat gaaggccggt cgagaaaaaa tcgcgccgac acggtcttgt
     4081 ctttggcgca tgcgagcttc tgaacggacg aaaaaaccgc gaaatgcgtg tcgacgaaat
     4141 ggcggcagaa tgtctcgctc cccaacactg cccgcgccac catgtccctg tcccgagaaa
     4201 aacgtcttga aactatcctt ggcgttaatc agcaaatagg cgtccacggg tttcaccggg
     4261 ttgacatagg cttcgacatt gttcggaccg acatccaatc cgttgagcct cgaccccgcc
     4321 ttagcggcga gacggagaca tttgacagcc ctgtcatggc tttgatccgc gtccttttga
     4381 ccaaccccat aagcgcaata ggaggccgtc gccaaggcgt atgcgagatt gtaatccggc
     4441 tcggggccgg ctgctgtctg ggcgcgggcg cttcccatcg acgcc
//
LOCUS       DATMPU010000288         3447 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_4231323,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000288 DATMPU010000000
VERSION     DATMPU010000288.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 3447)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3447)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3447
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4231323"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..795)
                     /locus_tag="VNY06_06545"
     CDS             complement(<1..795)
                     /locus_tag="VNY06_06545"
                     /inference="COORDINATES: protein motif:HMM:NF020387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RES family NAD+ phosphorylase"
                     /protein_id="HWX14500.1"
                     /translation="MRRFKSLNSFHDFERSVKRELRFVRNPDQENFLKTVIATSQSRA
                     TTLPKGFNTLWRARIGHDWIDREVNGGRFKERRAYKPQCMKPCPNKAGDGRANPRGIP
                     CLYMATLKTTAILEVRPWIGAYVSIAQLCLSEEVKIIDCTQDDFDYFDYTHGTISQDG
                     TIKKLARKEELEENEKMVWGGINRAFSMPSQREDESIDYIPTQILAERFKLSGFGGIA
                     YKSNFGGEKGFNIALFNLDAAEVVGCVMLCRVDDMSIKITTDRDGFG"
     gene            complement(870..1391)
                     /locus_tag="VNY06_06550"
     CDS             complement(870..1391)
                     /locus_tag="VNY06_06550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017501690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HWX14501.1"
                     /translation="MHNAGMIYGYARVSTDAQDLTSQLAQLKAAGCEKIFREKITGTT
                     ADRPQLKKLMHDLAPGDIVITPAVDRLSRDVTDLLVIARDMQRAGAGLRSIAEPVVDT
                     TSDFAELVLAMLGVAAKLERRRIAERTARGRADAKAKGVKFGRKPALTSHQQQEARKR
                     LIEGETQRSVARS"
     gene            1925..2419
                     /locus_tag="VNY06_06555"
     CDS             1925..2419
                     /locus_tag="VNY06_06555"
                     /inference="COORDINATES: protein motif:HMM:NF025292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin-like domain-containing protein"
                     /protein_id="HWX14502.1"
                     /translation="MNRRNIFGLSIIAVCGLALLPANALSQSKSLKDQLIGTWTLVSV
                     VEVYQDGRKENPWGPAVKGAVSFDPKGNVTLAIIGGDVTNPSGKPNESNRQVVAYFGK
                     YVVDEAAKTVTYTAERATEPSFDGLARKASVTVEGDELKQASAPITAPRGTFTPNLVF
                     KRAN"
     gene            complement(2539..3114)
                     /locus_tag="VNY06_06560"
     CDS             complement(2539..3114)
                     /locus_tag="VNY06_06560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011638900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HWX14503.1"
                     /translation="MLLGYARVSTIDQNLALQRDALAEAGCGRIFTEQLSGAVADRPA
                     LRDALEFARSGDTLIVWKLDRLARSMKQLIETIEELRLKGIGFRSLTEALDTTTAQGR
                     LVFHMFGALAEFERSLIRERTQAGLAAARRAGRTGGRPPKLTDDDIEAAKAMLANPDI
                     GVTQIAHRLGVSPATLYRYIPAARTANTPGV"
BASE COUNT          695 a         1004 c          979 g          769 t
ORIGIN      
        1 cccgaacccg tctcgatctg tagtgatttt tatactcata tcgtctactc tacaaagcat
       61 aacgcaacca accacctcag ccgcatcgag gttgaatagc gctatattga atcctttttc
      121 ccctccaaag ttgcttttgt aagcaatccc accaaatcca ctcaacttga atcgctctgc
      181 taaaatttga gtcggaatgt agtctatgct ttcatcttct ctctgtgacg gcattgagaa
      241 agcacgattg ataccacccc aaaccatttt ttcattctcc tcaagttctt ctttccgcgc
      301 aagcttcttt attgttccgt cctgcgaaat cgttccatgg gtataatcga aataatcaaa
      361 atcgtcctgc gtgcaatcga ttattttaac ctcctctgat aggcagagct gcgcaatcga
      421 tacataggca cctatccatg ggcgcacctc caatattgcc gttgtcttga gcgtggccat
      481 gtataggcag ggaatgcccc tcggattcgc ccgcccatca ccagccttgt tcgggcatgg
      541 tttcatacat tggggtttgt aggcccggcg ctctttgaac ctcccgccat tcacctctcg
      601 gtctatccag tcgtgcccta tccgggctcg ccaaagtgtg ttgaagcctt ttggaagtgt
      661 cgtcgcgcgt gattggcttg tagctataac cgtctttaag aaattctcct gatcggggtt
      721 tctgacaaag cgaagctccc tttttactga acgctcaaaa tcgtggaagc tgttcagcga
      781 cttaaatctt cgcatcagcg gggcatcccc ttcgctgcgg tgagggacag ctaggccgtg
      841 agccgtgaaa tcgtcgcctg actgacgttt tagctgcgcg cgacgctgcg ctgtgtctcg
      901 ccttctataa gccgcttccg ggcctcttgc tgctgatggg aggtgagggc aggcttgcgg
      961 ccgaacttga cgcctttggc tttggcatcg gcccggcccc gcgccgtgcg ctcggcgatg
     1021 cggcggcgct ccagcttcgc cgcgacgcca agcatggcaa ggacaagctc ggcgaagtcg
     1081 gacgtggtat cgacaaccgg ctcggcaatc gaacgcaggc ccgccccggc gcgctgcatg
     1141 tcgcgggcaa taaccagaag gtcggtcaca tcgcgcgaga ggcggtcaac ggccggggtg
     1201 atgacgatat cgccgggcgc gaggtcgtgc atgagtttct taagctgcgg tcggtcggcg
     1261 gtcgtgccgg tgatcttctc gcggaatatt ttttcgcaac cggccgcctt gagttgggca
     1321 agctggctgg tgaggtcttg ggcgtccgtc gagacgcggg catagccgta aatcatgccc
     1381 gcattgtgca tcagaaatgc gcatcaaaaa attgcaattc catcagcacg ttggttttga
     1441 accggatatc acggctgcag gttggcggat tctacggcga ccgatcatac acgagcgacg
     1501 cgtttcgacc cttctgagac attcctgatc aagcggagat ccgcggcgcg ccggccctgt
     1561 tcgccccaca gccggccgac tttctagcaa acaggtggcc ggcatcatcg gaatccgcgc
     1621 gaatttcgct gtgatgatcc cccagggggc ggaaggtcat gtcccatcgg ccgggtatcc
     1681 gggtatgtcg gttgaaaggc tagggctgca tattcggact tgttgccacg gatgcggcaa
     1741 gaggttcacg atggttttcg ctagacctag aagcggggac aataccggcc agcgtgccag
     1801 ccgtcgctgg aagacactgc cgagcgagcc ccgtccgtag gtcccggcaa tggtcgaatg
     1861 gtgcaccgag atgaggccct tgcagcgttg cccagatcat gacagcgaac ccgggaggaa
     1921 aatgatgaat cggaggaata ttttcggact gtctattatt gccgtgtgcg ggctcgcctt
     1981 gcttcccgcc aacgcgctga gccagtcaaa atcacttaag gatcagctca tcggaacttg
     2041 gacactggtt tccgtagtcg aagtctacca ggacggcagg aaggaaaacc cctgggggcc
     2101 ggcggtcaag ggcgccgtca gcttcgaccc caagggtaac gttaccttgg cgatcattgg
     2161 tggagacgtg acaaacccat cgggcaaacc taatgagtcg aaccggcagg tggtcgccta
     2221 tttcggcaaa tacgtcgtcg acgaagcggc gaaaaccgtc acctataccg ccgagcgtgc
     2281 gaccgagccg tcattcgacg ggctcgctcg aaaggcgtcg gtgacggtgg agggcgatga
     2341 actaaagcaa gcctcggctc cgattacggc gccgcgagga acgttcacac ccaatcttgt
     2401 cttcaaacgg gcgaattgag attcagagca gcgcccacaa gtcggcaaat cggcaacggc
     2461 ggatttccac ccattgccga cgcctcaacg acaccccgcc cccttgcggc gtgcggcctt
     2521 tgggatagag cgccgttctc aaacgcccgg ggtattcgcg gttcgtgcgg cggggatgta
     2581 gcgatagagc gtcgccggag agacgccgag gcggtgcgcg atttgagtta cgccgatgtc
     2641 gggattagcg agcatcgcct tggcggcctc gatgtcgtcg tccgtcagtt tcggcggacg
     2701 gcctccggtg cggcctgcgc gccgcgcggc ggcaaggccc gcttgggtgc gctcccggat
     2761 caggctgcgc tcgaattccg ccaacgcgcc aaacatgtga aaaacaagcc gaccttgcgc
     2821 ggtcgtcgtg tcgagggctt cggtcaggct gcgaaaaccg atgcctttca gccgcagttc
     2881 ctcgatggtt tcgatcaact gcttcatcga gcgcgccaag cggtcgagct tccacacgat
     2941 cagcgtgtcg ccgctgcgcg cgaattccag cgcgtcgcgc aaggctggtc gatccgcaac
     3001 cgcgcccgac aattgctcgg tgaaaatcct cccgcatccc gcttcggcaa gcgcgtcccg
     3061 ctgcaaggca agattctggt caatggtcga aacccgcgcg tagccgagca acataccttt
     3121 cgacgatcca tcctcgccgg gtcgcggcgc tgaaattgct cgcgcgacct tgcgcttggc
     3181 ggggcgtcct cgtttgctca gcgaggccgt acgagcgatt ctcgctcttc tcttctgcat
     3241 gaaccgctct ctctaaactc agccgaccat agactttttg agaggctgaa atcaagaatg
     3301 agttttgaga gacgtttaag ccgtttttcg tgttgattgt tccgtttcta tccaccctct
     3361 caaaaaccct cgtttttgag aggcaaagcc aatgtgcatg agtcttgcag accgtatgaa
     3421 aactcgtgat tgattcataa acaggct
//
LOCUS       DATMPU010000289         7437 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_1161627,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000289 DATMPU010000000
VERSION     DATMPU010000289.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 7437)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7437)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7437
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1161627"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..363)
                     /locus_tag="VNY06_06565"
     CDS             complement(<1..363)
                     /locus_tag="VNY06_06565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012589540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HWX14504.1"
                     /translation="MAEIDQGQRGNRDGNLAEAMRIAEALLFAASEPLSEKDIERRMP
                     RGLAGAKILEALKVEYASRGVNLIRAGDKWMFRTASDLAWLLATGEVEEKRLSRAALE
                     TLAIVAYHQPVTRAEIEDI"
     gene            complement(420..1223)
                     /locus_tag="VNY06_06570"
     CDS             complement(420..1223)
                     /locus_tag="VNY06_06570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012589539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ScpA family protein"
                     /protein_id="HWX14505.1"
                     /translation="MDLPIADRTAADTEEDGSFVVDVGGFEGPLDLLLDLARRQKVDL
                     GKISVLALAEQYLEFIAAARSLRIELAADYLVMAAWLAYLKSRLLLPQAAKDAGPEGA
                     EFAGALQFRLRRLEAIRAAAEALVNRPRLGRDVFLRGLPEPVLPQGAPQWRASLYEFL
                     CAYTNRRQKQALTRVAVRQRFVWSLAQARDQLEKLAGCALDWTVLDTYLAAFCATPEM
                     SRTVTASTLSASLEMAREGVISIRQDEAFAPLWIKRKQAAGTFPKSVQT"
     gene            complement(1457..2857)
                     /locus_tag="VNY06_06575"
     CDS             complement(1457..2857)
                     /locus_tag="VNY06_06575"
                     /inference="COORDINATES: protein motif:HMM:NF016895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HWX14506.1"
                     /translation="MSEPLVKRRPMIDLEEFERRLRKPLSLNQEDHDPLAELARFVGG
                     QEDSFREDPYKTVFEAHNGRPAGALRGGQDHGEVREAGVQEPLIRGDFASIEAGLLGA
                     DLPEDEFNPSANIVLDHSEDMDDAAHLPYPETEDDASEGSAPYEEIRSRRPLYVMAAM
                     IIAGVAGIFASFAFKGTVSGPDEVATIRAVDGPVKVQPETAAGNENPSQDATVLGGLP
                     QQPPVAAVSNIEQPSDLSAQTEVAPAQGTLQSSVTGVAGGAASVPVPVPPAQAQPRPP
                     NEPQSIAELIEPKKVKTVSVRPDGTLLPNDTPPQAAERAAPVPVARPATLPAAKAVTP
                     KSAVRVATTPKPVVEAANGNPQTAQTSVAPKARRPESLAVHERGQTGEPSSGTFAVQL
                     AAPGSEQEARDLQARLMQKFGSELAGFKPSIRKAEVGGKEVYRVRFSGAASREQAAAL
                     CQKVQSSGGSCFVAKN"
     gene            complement(3198..4976)
                     /gene="argS"
                     /locus_tag="VNY06_06580"
     CDS             complement(3198..4976)
                     /gene="argS"
                     /locus_tag="VNY06_06580"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018265464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HWX14507.1"
                     /translation="MNLYAEIHDQIVAILHRISASGRLPSGLDLQRFVVEPPRDSSHG
                     DLATNVAMAYAKEARSFFANPRQLAVEIATALADYPTVAAAEVAGPGFINLRLKPATF
                     SRVLRAALELGKDFGRPAPGSITRLKINIEYVSANPTGPMHVGHGRGAVFGDALANLQ
                     EFVGHEVTREFYINDAGAQVDVLARSAYLRYREALGEEIGEIPEGLYPGNYLKSAGQA
                     LAEKYGRKFLARREAEWLDEVRGEVIDAMMAMIRADLAALNIEHEIFFSERSLTSGVE
                     GDLVAKAIAELRRRGLIYEARLPRPKAQLVEDWEDREQILFKSTAFGDDIDRPLMKSD
                     GSYTYFASDIAYHKIKIDRGYALLIDVWGADHGGYVKRMHAAVEALSQGRTRLDVKLC
                     QLVQLMRNGEPVKMSKRSGDFVTLRDVVDEVGVDAVRFMMLFRKNDAPLEFDLSRVIE
                     QSKDNPVFYVQYAHARAKSVLRLARSAIFERDISTSALAVADLSLLQDEGEMALIKLI
                     AQYPRIVVSAAQNDEPHRIAFYLHDLASCLHGQWTRGKDRPQLRFLNEERTDLTLARL
                     ALVAALAAVLASGLEILGVSAPEEMR"
     gene            complement(5050..6315)
                     /locus_tag="VNY06_06585"
     CDS             complement(5050..6315)
                     /locus_tag="VNY06_06585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020175639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016793 - triphosphoric monoester
                     hydrolase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyguanosinetriphosphate triphosphohydrolase"
                     /protein_id="HWX14508.1"
                     /translation="MPAVHFSSGHLPIPARAPYATDAARSRGRLISEPDSPSRSPFQR
                     DRDRIIHSTAFRRLAHKTQVFVPHEGDHFRTRLIHSLEVGQIARSTARALGLDDDLAE
                     ALALAHDLGHTPFGHTGEDVLNELMAPYGGFDHNAQALRIVTKLERRYAAFDGLNLTW
                     ETLEGLVKHNGPLLKPDGGLAEPYEKRGLAGAILEFNAAFDLKLSHCASAEAQAAALS
                     DGVAYNAHDLDDGLRAGLINFRQIGEVDFLRAMSQDISALYPRIEPFRTIHELVRRMI
                     TFFIEDAIVESRRRIEASGVTSAEDVLAQKAPLIGFSRAALNADASIRNFLRENIYCH
                     KRLAHIRRDAGQIVRNLFIRFFAAPDLMPEEWALAAAKDDAGEARRARMVCDYIAGMT
                     DRYAVAEHRRLFAETPDLQLGLSEQKPSR"
     gene            6458..6790
                     /locus_tag="VNY06_06590"
     CDS             6458..6790
                     /locus_tag="VNY06_06590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012332327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly accessory protein"
                     /protein_id="HWX14509.1"
                     /translation="MSDAPIELTENAARRVAEILRQEPAGSFLRIGVDGGGCSGFSYT
                     YGISMEHIAGDLVIKRDGATVAIDPVSLDLLRGSKVDFVDGLMGRMFKIDNPLATASC
                     GCGSSFAV"
     gene            6911..>7437
                     /locus_tag="VNY06_06595"
     CDS             6911..>7437
                     /locus_tag="VNY06_06595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020187971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H-like domain-containing protein"
                     /protein_id="HWX14510.1"
                     /translation="MTIRFHQGDLPDEAGFGDAVAIDTETLGLVPRRDRLCVVQFSCG
                     DGTADIVQIAPGQTEAKNILRILADPSITKLFHFARFDLAILFHTFGVMAAPVYCTKI
                     ASKLARTYTDRHGLKDLVRELLGIELSKQQQLTDWGGETLTDAQISYAASDVLHLQAL
                     RQRLDLMLEREGRLS"
BASE COUNT         1516 a         2220 c         2121 g         1580 t
ORIGIN      
        1 tatatcctca atctcggccc gcgtgacggg ctggtgatag gccacaatgg ccagggtctc
       61 gagcgccgcc cgcgagagcc ttttttcttc gacttcccct gtagcgagca gccaagcaag
      121 atcggatgct gtccgaaaca tccatttgtc acctgccctt atgagattga cgccgcgcga
      181 cgcatattca acctttagcg cttcaagaat cttggctcca gcgaggcctc ggggcattcg
      241 cctttcgatg tctttttcag aaagcggttc tgaagcggcg aacaatagcg cctccgcgat
      301 ccgcatcgct tccgccaaat tgccgtcacg attccctctc tggccttgat caatttcagc
      361 caatagctgc gcaagttctg ccaacaaagc cctcctcatc cgctttgcac gaagtctgtt
      421 caggtttgaa ccgatttcgg gaaggtcccc gcagcttgct tccgcttgat ccagagcggc
      481 gcaaacgcct cgtcctggcg gattgagatg acgccctccc gggccatttc aagcgaagcg
      541 gaaagtgtcg aagccgtgac cgtgcggctc atctcgggtg tagcacaaaa ggcggcgaga
      601 taagtatcaa gcaccgtcca gtcgagcgca cacccagcaa gtttttcaag ttggtcgcgc
      661 gcctgggcga gcgaccaaac aaagcgctgc ctgacggcta cccgcgtaag cgcttgtttc
      721 tggcgccggt tcgtgtaggc gcataaaaat tcataaaggc ttgcgcgcca ttgcggcgcg
      781 ccttggggaa gaactggctc aggaaggccg cgcaagaaca cgtcgcggcc aagacgtggg
      841 cggttaacaa gcgcttcggc ggcggcgcgg atcgcctcaa ggcggcgaag ccgaaactgc
      901 agcgctccgg caaattccgc gccctccggc cctgcatctt tcgcagcctg cggaagtagg
      961 aggcgcgact tcagataagc gagccaagcc gccatcacga gataatcggc ggcaagctcg
     1021 atccgcaggg agcgcgcggc cgcgataaat tcgagatatt gttccgcaag cgcaagaacg
     1081 gaaatcttgc ccaaatccac tttttggcgg cgcgcgaggt caagcaagag atcgagcgga
     1141 ccctcaaatc ccccgacatc gaccacgaag gagccatcct cctccgtgtc cgctgccgta
     1201 cggtccgcga ttggcaagtc caacggttca cactcccagt cgactgcttg cgcttcaggt
     1261 ccctggtgag gcgcaacaaa ttctaatttt atccgcgcgc ccacccttgc ggcaacgccg
     1321 acacccgccg tggggctact agctccacag gattacgacg ggagagccgt gccgctaccg
     1381 tcgtggcttt cccgctcgaa cgaacgggct caccccatga acgcttcctg cggaagcgcc
     1441 atgtaatgcc cctggctcag ttcttggcga cgaagcaact gccgccgctg ctctgcactt
     1501 tttggcacaa tgccgcggcc tgttcacgcg aggccgctcc actgaacctt acgcgataga
     1561 cttccttgcc gccgacctct gccttgcgga tcgaaggttt aaagcccgcc aattctgaac
     1621 cgaatttttg catgagccgc gcctgcaggt cccgagcctc ttgctcagaa cctggagccg
     1681 cgagctgcac cgcgaaagtt cctgacgacg gctcgccggt ttgtccccgc tcatggacag
     1741 ctagagactc cggacgcctg gccttcggtg cgacggaggt ctgggccgtt tgtggattgc
     1801 catttgcagc ttcgaccact ggcttgggag tcgtcgccac gcgcacagcc gactttggag
     1861 tgacagcctt tgccgccggc agcgtcgcgg ggcgggcgac cgggacgggc gcggcacgct
     1921 ctgcagcctg cggcggtgta tcgtttggca gcagcgtgcc gtcgggccgg accgaaaccg
     1981 ttttgacctt tttcggttca atcagttcgg caatactttg cggctcgttg ggcggtcgcg
     2041 gctgagcctg tgcaggcggc accggcacgg gaacgctcgc cgccccgcct gctacccccg
     2101 tcacagaact ttgcaaagtc ccctgtgctg gggccacttc cgtttgcgct gacaaatcgg
     2161 agggctgttc gatgttgcta actgccgcga cgggtggctg ttgtggaagt ccgccaagaa
     2221 cagttgcgtc ttggcttgga ttctcattcc ccgctgcggt ttcgggctgt accttcacgg
     2281 ggccgtcaac ggccctgatc gtagcgacct catccgggcc ggaaaccgtg cctttgaacg
     2341 caaaactcgc gaagatgcca gctacgccgg cgatgatcat ggcggccatc acgtagagcg
     2401 ggcgccgcga tcgaatttcc tcgtagggag cggagccttc gctggcatca tcttcggtct
     2461 ccgggtaggg caaatgagct gcatcgtcca tatcttcgct atggtccagc actatattgg
     2521 cggacgggtt aaattcatcc tcgggcaggt cagcgccaag cagtccggcc tcgattgagg
     2581 cgaaatcgcc gcgaataagt ggttcctgga cgccggcttc tcgaacttcg ccatgatcct
     2641 gcccgccacg caaagctcca gcaggccgcc cgttatgcgc ctcaaacacg gtcttatagg
     2701 ggtcttccct gaaagaatct tcttgcccgc caacaaagcg ggcgagttcg gcaagcgggt
     2761 catggtcctc ttgattcagg gaaaggggct tccgcagacg ccgttcgaat tcttccaaat
     2821 ctatcattgg acgccttttt actagtggct cgctcatctt acgcaccctc ctgtcgcgac
     2881 ttcatggcgg acaaaaccgc cggtccacat caccgcaatc atcgcaatcg cgcatctcac
     2941 gcggggcaat tgtcacacac tcccaagcga tccggccgga tttccgttaa ttgtaacgat
     3001 tattgtaacc tgatccttgt cactttgcac gcgtcgattg ccactcaatt catccgcgac
     3061 ctgattattt gtgcacgtta agcgccccgc tttttcgctg cagagctgta gtgtcacgct
     3121 tacgtcggca tacccaagcc ggcggctgcg atagagttgc tagcagcgca cctttataga
     3181 gatgcgagaa aaggctgtca tcgcatctct tcaggggcgc tcacgcccaa aatctcaagt
     3241 cccgacgcga gcactgccgc cagtgcagct acgagagcca gccgagcgag agttaaatct
     3301 gtacgctctt cattaagaaa gcgtaattgt ggccgatctt tgccccgagt ccattgcccg
     3361 tgcaggcacg aggcaagatc atgcaaataa aatgcaatcc ggtgcggctc gtcattctgc
     3421 gccgccgaca cgacaattcg gggatattgc gcgatgagtt tgattaatgc catctcccct
     3481 tcgtcctgca acaagctgag gtctgccacc gccagagctg aagttgatat gtcacgctcg
     3541 aaaatcgctg agcgcgcgag ccgcagcacg gatttggccc tcgcgtgggc atattggaca
     3601 taaaagaccg gattatcctt ggattgttcg atcacgcggc tcagatcgaa ttccaagggt
     3661 gcatcgttct tgcgaaaaag catcatgaag cggaccgcat cgacgccgac ctcgtcaacg
     3721 acgtcccgca aggttacaaa atcgcccgat ctcttcgaca ttttcaccgg ctcgccattg
     3781 cgcatcaatt gaaccaattg gcaaagcttg acgtcgagcc tggtgcggcc ttgcgaaagc
     3841 gcctcgacgg cggcgtgcat ccgtttcaca tagccgccat gatctgcgcc ccaaacgtcg
     3901 atcaaaagcg cgtagccgcg gtcgatcttg atcttgtggt aggcaatatc agaggcgaag
     3961 taagtgtaag atccatctga cttcataagt ggccggtcga tatcatcgcc gaaggccgtc
     4021 gatttgaata aaatttgctc gcggtcttcc caatcctcga caagttgcgc ttttgggcgt
     4081 gggagccgcg cttcgtagat gagaccacgc cggcgcagtt cggcgattgc ctttgccacc
     4141 aaatcgcctt ccaccccact ggtgagtgaa cgctccgaga aaaaaatctc atgctcaatg
     4201 ttaagggccg caagatcggc gcgaatcatc gccatcatgg cgtcgatcac ttcgccgcgc
     4261 acctcatcaa gccattcagc ttctcggcgc gctaaaaatt tgcggccata tttttcggcg
     4321 agtgcctgcc ccgcgctttt gaggtaattt ccgggataaa ggccttcggg gatttcaccg
     4381 atctcttcgc ctagcgcttc cctgtagcgc agataagccg agcgggccaa aacatcaacc
     4441 tgggcacctg catcgttaat ataaaattcc cgcgtgacct catgacccac aaactcctgg
     4501 agattggcga gcgcgtcgcc aaataccgcg ccacggccgt ggccgacatg catcgggcct
     4561 gttgggttgg ccgaaacata ttcgatgttg atcttaaggc gcgtgatgct gccgggagcc
     4621 ggcctgccaa aatcttttcc aagctccagc gccgcgcgca aaacgcgcga gaatgtcgca
     4681 ggtttgagcc gaagattgat gaagccgggc cctgccacct ctgcagccgc aacagttgga
     4741 taatcagcga gcgccgtggc gatttcgacg gcaagctggc gcgggtttgc gaaaaaggac
     4801 cgcgcttcct ttgcataagc cattgcgaca tttgtggcga ggtcgccgtg cgaggagtct
     4861 cggggaggct cgacaacaaa gcgttgcaag tctaggccgg acgggaggcg tccgcttgca
     4921 gagatacggt gcaagatagc aacaatctga tcgtggatct cggcataaag attcatagag
     4981 caccaacttt ttcagcctga tccaaggcac tttgaaaatt gcgcggcgcg ctgcggattt
     5041 gaaagccgcc taacgagacg gcttctgttc ggaaaggcct aactgcaaat ccggagtttc
     5101 ggcaaaaagt ctccggtgct cggcaacggc atagcggtcg gtcatcccgg caatgtagtc
     5161 gcagaccatg cgggctctgc gcgcttcgcc ggcgtcatcc ttggcggcag ccagggccca
     5221 ttcctctggc atcaaatctg gagccgcaaa aaaccggata aagagattgc gaacgatctg
     5281 ccccgcgtct cttcgaatat gggcgaggcg cttatggcag tagatgtttt cgcggagaaa
     5341 gttccgtatc gacgcatccg cattcagcgc cgcgcgggaa aagccaatca agggcgcctt
     5401 ttgcgccaag acgtcctccg cgctggtcac gcctgatgcc tctatccgcc gccggctttc
     5461 gactattgca tcttcaatga agaaagtgat catcctgcgg acgagctcgt gaatggtgcg
     5521 gaacggctca atgcggggat aaagcgcgct gatgtcttgg ctcattgcgc ggagaaaatc
     5581 cacctcgccg atttgccgaa agttaatcaa gcccgcgcgc aatccgtcgt cgaggtcgtg
     5641 tgcattgtac gcgacaccat cggagagagc cgccgcctgt gcctcagcgc tggcacaatg
     5701 cgaaagcttg aggtcaaacg ccgcattaaa ttccagaatt gccccggcaa gcccgcgttt
     5761 ttcatagggt tcagccaagc cgccatcggg tttcaagagc ggcccgttat gcttcacgag
     5821 cccctccagc gtttcccaag tcagatttag cccgtcgaaa gccgcgtatc ggcgctcgag
     5881 cttggtcacg atccgcagcg cctgggcatt gtgatcaaag ccaccgtagg gagccattag
     5941 ctcattgagc acatcctcgc cggtatgacc gaagggggta tggccaaggt catgggcaag
     6001 cgcgagcgcc tccgcgagat cgtcgtcgag gccgagcgcg cgggcagtcg agcgcgcgat
     6061 ctgcccaacc tcgagcgaat ggataagcct tgtccggaaa tgatcgcctt cgtgcggaac
     6121 gaagacctgc gtcttgtggg cgagtctgcg gaaagccgtg ctatggatga tacggtcacg
     6181 atcgcgttga aatggagagc gcgaggggga atccggctcc gagattagcc gtccacggct
     6241 tcgcgccgca tcggtggcgt aaggggcgcg cgcgggaatt ggtaaatgac cggaagaaaa
     6301 gtggacggca ggcatggact cttggaaatt cggcgcgcca attgcggctc ttgttcgccc
     6361 ggcttatgtt ccttatcgcc ggcgggcgca agcggctctc gcgggctaca caaccggggt
     6421 ttcagcggct gaatttattc ttgagaggtc tccagacatg agcgatgcgc cgatcgaact
     6481 taccgaaaac gccgcccgtc gcgttgcgga gattttgcgg caagagcctg caggctcctt
     6541 cttacgaatt ggagtcgacg gcggcggatg ctcgggattt agctacacct acggcatttc
     6601 gatggaacat atcgcgggag atctcgtcat caaacgtgac ggcgcaaccg tcgcgatcga
     6661 ccccgtttcg cttgatctcc tgcgcgggtc aaaggtcgac tttgttgacg ggcttatggg
     6721 ccgcatgttc aaaatcgaca atcctttggc gacggcgtcc tgcggctgcg ggtccagttt
     6781 tgcggtctag cctggtttcg gggcgatgcg cttgcgatgt tttacagcaa tgctacccgc
     6841 ccttgatgga tcctccctgg ccatgcttaa aatcgcggtt cgcgcgccgc gcgaccgctg
     6901 gcgctaggcg atgaccatcc gctttcacca gggtgacttg ccggatgaag ccggctttgg
     6961 cgacgccgtt gcgatcgata ccgaaacctt gggcctcgtt ccccgaaggg accggttgtg
     7021 cgttgtccaa ttttcctgtg gggacggcac cgccgatatc gtccaaatcg cgcctggcca
     7081 gacggaggcc aagaatatcc tgcggattct tgccgaccct agcattacaa aattgttcca
     7141 tttcgctcgt ttcgacctcg caatattatt tcacacattc ggtgtcatgg ccgcgcccgt
     7201 ttattgtacc aagatcgcgt ccaagctcgc gcggacctac accgaccggc acggtctgaa
     7261 ggatttggta cgtgagcttc tcggtatcga attgtcaaaa cagcaacaat tgaccgattg
     7321 ggggggggaa acgctgaccg atgcgcagat ttcttatgct gcgtccgacg ttcttcattt
     7381 gcaggcgttg cgccagcgcc tcgacttgat gctcgagcgg gaaggtcgct tgtcgat
//
LOCUS       DATMPU010000290         4653 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_851306,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000290 DATMPU010000000
VERSION     DATMPU010000290.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 4653)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4653)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4653
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_851306"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(127..531)
                     /locus_tag="VNY06_06600"
     CDS             complement(127..531)
                     /locus_tag="VNY06_06600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009493068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWX14511.1"
                     /translation="MARTEHLCQDCAATRRRCVLVVEDEILVRLTIADHLRGAGYEVV
                     EAANATEAIAVFASGELVDVVFTDVQMPGAMDGLMLARWVHEHHPGIHVLVTSGKGDA
                     AVSSGLVPDGAFFSKPYPLEAVATRIQSLLDE"
     gene            complement(1145..1588)
                     /locus_tag="VNY06_06605"
     CDS             complement(1145..1588)
                     /locus_tag="VNY06_06605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016920556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PA2169 family four-helix-bundle protein"
                     /protein_id="HWX14512.1"
                     /translation="MDKVISTLNTLLETTKDGEHGFRTCAEAVTNPRLKTMFEDAARR
                     CDGGAAELQAKIRSLGGEPATSGTASGALHRAWTNIKSSITGMDEYAVLTECERGEDV
                     AKSAYEEALKEDLPMDIKTLVQRQYEGVKANHDRIRQLRNQAAHL"
     gene            2141..2320
                     /locus_tag="VNY06_06610"
     CDS             2141..2320
                     /locus_tag="VNY06_06610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018265491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HWX14513.1"
                     /translation="MGNLLYWAVVFLIVALVAAALGFGSVAGVAIEGARMLFWVAIVL
                     FVVALVGGLIRRGGV"
     gene            complement(2639..3778)
                     /gene="rseP"
                     /locus_tag="VNY06_06615"
     CDS             complement(2639..3778)
                     /gene="rseP"
                     /locus_tag="VNY06_06615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020176462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HWX14514.1"
                     /translation="MSVLASVWSLAGYILPFLFVLSLVVFFHELGHFLIGRWCGVKVE
                     TFSLGFGPELFGFNDHVGTHWRIAALPLGGYVKFHGDANGASMPSESAMEAMSPQERA
                     VSFSTQKVWKRAAIVAAGPVANFILAIAIYTGIFYLHGRAILLPEADSIAADSAAEAA
                     RFQPGDLIVAIDGARVESFEDMQRIVQTASGTQLVFTVDRAGKSVELVATPRRRDLVT
                     PFGTTRGGVLGVKANPANLHVQTYDLLESVKLAGSETWYIVARTGSYLKGLAAGEEST
                     DQLSGPIRIAEVSGAMARIGLAALLTLAAALSISVGMLNLLPVPLLDGGHLVYYAVEA
                     MRGKAMNERAQQFGFDVGVVLLTSLMFFATYNDILRLARHWMHGE"
     gene            4168..4419
                     /locus_tag="VNY06_06620"
     CDS             4168..4419
                     /locus_tag="VNY06_06620"
                     /inference="COORDINATES: protein motif:HMM:NF017758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF892 family protein"
                     /protein_id="HWX14515.1"
                     /translation="MRSMEELFYALLQDVYFAEKQLVKTLPKLAKNSANEKLQEAFIN
                     HYDETEEQVARLEKVFEAIGLTTARLTKISDHRIGSSRC"
     gene            complement(4425..>4653)
                     /locus_tag="VNY06_06625"
     CDS             complement(4425..>4653)
                     /locus_tag="VNY06_06625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006337482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF892 family protein"
                     /protein_id="HWX14516.1"
                     /translation="EYKGSPALDAGLLAAAQAVEHYEISRYGTLRAWAEELGLNEAVE
                     LLQETLEEEEATDEALTEIAKAAINQRAEAA"
BASE COUNT         1019 a         1365 c         1280 g          989 t
ORIGIN      
        1 agacgccacc cgtcagagcc ggcgacaaga aatgtcatcc ctaacgtctc aattgcacat
       61 gccctggctc ttggtcgcta acgtgtcaat gcccaacgaa agctactcgg ccgggaacta
      121 actgcgttat tcgtccagta acgattggat gcgcgtcgcc actgcctcaa gcgggtacgg
      181 tttcgaaaag aaagcgccgt caggaacgag cccagaggag acggcagcat caccctttcc
      241 cgaagtcacc aacacatgga tgccgggatg atgctcatgg acccaacgcg cgagcataag
      301 cccgtccatg gcgccgggca tctgcacatc agtaaaaacg acgtccacca gctccccgga
      361 tgcgaagacc gcgatcgcct cggtcgcatt cgccgcttcg acaacttcgt atccagcgcc
      421 ccggagatga tcggcaatcg ttaatcggac cagaatctca tcttcaacga ctaaaacgca
      481 acgccgacgg gtcgctgcgc aatcttggca taggtgctcg gttctagcca tgcgtaataa
      541 acttcgcgcc atgctaaagc gatccacaca ggatcagaga agcctgattg tagccagact
      601 acaacttaag gattcaagga gtttcggcgt cgttcgcgga gggcgttgat ggtatggctt
      661 ctccgcatta agctccggcc gcgcggattg aaatcatgcc gagaaaagtg gcaaccccgg
      721 ccgcggtaac gcgaatgtcg atggcctgca ggaacgcaac atgatgatcg atgcgatgtc
      781 catgactgtt tcctcaccgt ttttcagatc agctcaaaac cagctagtgg gttgatagtt
      841 tcttggacgc ccgttcaaca tttcgtttaa cgggctccag caaatcgcga gtccagtctc
      901 gccaagggcg cgacgtgctc gcagcgcgag aacgcttttt cggacaccgg acttccgaac
      961 cgaaacgctt cggaggttca atgatagaaa agccacaaca cgatgattac tggaattgga
     1021 acaccaagca gccagagaag caaaccgcga cccataacac acctccttta attgcatgag
     1081 gcacaccgcc ccccttttgg cgggcggcgg ccctggcttg gaatccttac gattctggga
     1141 tgtttcagag atgggcggcc tgatttcgca gctggcggat gcgatcgtga tttgctttca
     1201 cgccttcgta ctgccgttgg acgagtgtct ttatgtccat aggcaggtcc tcttttagcg
     1261 cttcttcata ggcgcttttc gccacgtctt cgccgcgctc gcattctgtc agcaccgcat
     1321 actcgtccat gccggtaatc gaagatttga tgttggtcca cgcgcgatgc agcgcaccgc
     1381 tcgccgtgcc tgaggtagct ggctcaccgc cgaggctgcg tatcttggcc tgcaattcag
     1441 cagcgcctcc atcgcatctc cgggcggcat cctcgaacat cgtcttcaaa cgagggtttg
     1501 tgacggcttc tgcgcaagtg cggaagccgt gctcaccgtc ctttgtcgtc tccagcagtg
     1561 tgttcaaggt gctgattact ttgtccattt gctttctcct gtctaaccta gtgcgaagtg
     1621 ttcgacgatt ccccaccgca tcgccgttgg cgaaattccc tccctgcgat tgaccgccct
     1681 cgcggccaat ctcctcgggt ggcttatgag cttgtgagaa gcttcgcttc tcgtcgggaa
     1741 cgctcggcgc cctttgcggc cagtttccgg ctcaaggccg tgatgattgg caaagcttcg
     1801 cttctcgccg ggcactctcg ctccgctgtc gcaggcaaat ttccaatgtt tgtcttcgcc
     1861 cgccgaagcg aagccaggat tcggcaggtt gctggccatc attggtctcc atcgctggtc
     1921 tccatcgctg caataatgcc gccgctccaa ccgggagctc gccgctgatt cttgcaattc
     1981 acgccaatga gatatcgttc ccaacccaca cactaatgtg aagacagacg gccacgctgg
     2041 aaaagattca gtttgggttt agccaaactc ccgtgaaagc tgaaaccctt ttgcgccacg
     2101 aacgttgacg ccatcaacgg gaacagaagc ggagaaagct atgggcaatc ttctttattg
     2161 ggccgtggtg tttctgattg tcgccctggt cgccgcggcg cttggatttg gcagcgtggc
     2221 aggagtggcg atcgaaggcg cccggatgtt attttgggta gcaatcgtgc tcttcgtcgt
     2281 cgccttggtt ggaggcctca taaggcgagg aggcgtctag cggcgccaaa gcatgcgagc
     2341 cggcggcgac gcgaaaacgc tgccggcttt tgtgcttggg cacgagacaa aggattcagc
     2401 tgattgaatg atgcagcgta gtcaatcgtc cgaaagtcaa agcgagctga tgaggtgggg
     2461 tgggtgcacg ggacgtgcca cgactcatcc ttctggcgtg ctctacccat gacttgcaac
     2521 cggcgcttac gctcataaag agtctcgatg ccggtgccgg gtcatcgggc caaagcgaaa
     2581 atcgccaatt cccggcgcgc cggtacagcc gcgcccagct gagaccggcc ggtgacgatc
     2641 attccccgtg catccagtgg cgtgccagcc ggagaatgtc gttgtaggtg gcgaagaaca
     2701 tcaagcttgt taacaagaca accccgacgt cgaatccgaa ctgctgcgct ctttcgttca
     2761 tcgccttgcc gcgcatggcc tccacggcat agtagacaag atgcccgccg tcgaggagcg
     2821 ggacggggag aagatttagc attccaaccg aaatcgacag cgcggcggcg agggtgagca
     2881 aggccgcaag gccgatcctg gccatcgctc ccgaaacctc tgcgatccgg atggggcccg
     2941 agagctgatc ggtagattct tcgcctgccg ccaagccttt gagatagctg ccggtgcgcg
     3001 ccacaatata ccaggtctcc gaacccgcga gcttgaccga ctcgaggaga tcataggtct
     3061 ggacgtgcaa atttgccggg ttggctttga cgccaagcac accgccgcgc gtggtgccaa
     3121 agggagtgac aagatcgcgc cggcgcggcg tcgcgacaag ctcgactgat tttccggccc
     3181 ggtcgacggt gaaaaccagc tgcgtgccgc tcgccgtctg cacaattcgc tgcatgtcct
     3241 caaaactttc aaccctcgcg ccgtcaatgg caacgataag gtcgcccggt tgaaacctcg
     3301 ccgcctcggc ggcgctgtcg gcggcaatgc tgtcggcctc tggaagcagg atcgcgcggc
     3361 cgtgcagata aaatatgcca gtataaatcg cgatcgccag aatgaaattc gcaaccgggc
     3421 ccgcggcgac gatggccgcc cgcttccaga ccttctgggt ggagaagctc accgctcgtt
     3481 cttgcggaga catcgcctcc atggcggatt cacttggcat tgaggcaccg ttggcatcac
     3541 catggaattt gacatagcct ccgagcggca gagccgctat tcgccaatgg gttccaacgt
     3601 ggtcgttaaa gccgaacaac tccggcccga aaccaagcga aaaggtttcg accttaaccc
     3661 cgcaccagcg gccgatcaga aaatggccga gctcatgaaa gaaaaccacc aagctcagga
     3721 caaaaagaaa aggcaaaata tacccagcga gcgaccaaac ggatgcgagc acggacattg
     3781 aactcctaaa tgcacagtcg gcatggcttg gaggccgttg cgatgcgatc atcaggggca
     3841 aacaccgagg ctgttgcagc cgaccttcgt aatccccttt ctgtactgcc ttctaattcc
     3901 agcgcgaaag gatagttcca ttcccgcgaa atggcttgac ccgacgattg gctttgtccg
     3961 ccgattctct agcctttcac gcgactctaa ggacggttgg acccatacgc gggtaaacag
     4021 atcgttaccg tcggcagtaa ccttgctctg cgccagacgg gcggaagtgc caacactgac
     4081 ggccatcgct atcgattgcc gaaggaacaa atccgcgtca ctggaattta tggccacatc
     4141 ggtccacctt gtagatagga gcatgtcatg cgatccatgg aagaactttt ttacgccctt
     4201 ctccaggacg tctattttgc cgagaaacag ttagtcaaga cgctgcctaa acttgcaaaa
     4261 aacagtgcga atgaaaaatt gcaggaggca ttcataaacc actacgacga gaccgaggag
     4321 caggtcgcac ggctcgaaaa ggtgtttgaa gccatcggct tgacgaccgc gcgcttgacc
     4381 aagatcagtg accatcggat tggttcgtcc cgttgttgat gatgtcaggc tgcttcggcc
     4441 cgctgattta tcgccgcttt cgcgatttct gtcagtgcct cgtcagtcgc ctcttcttct
     4501 tcaagagttt cttgcagtag ctcgacggct tcattcagac ctaattcttc tgcccacgcg
     4561 cgcagagttc catagcggga tatctcatag tgctcaaccg cctgagcagc ggcaagcaga
     4621 ccagcgtcaa gcgcggggga gcctttgtat tct
//
LOCUS       DATMPU010000291         3403 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_2372797,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000291 DATMPU010000000
VERSION     DATMPU010000291.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 3403)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3403)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3403
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2372797"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..149)
                     /locus_tag="VNY06_06630"
     CDS             complement(<1..149)
                     /locus_tag="VNY06_06630"
                     /inference="COORDINATES: protein motif:HMM:NF019997.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArdC family protein"
                     /protein_id="HWX14517.1"
                     /translation="MFAPVSAHRAAGSILRPVNIASKKAYRGVNVVALWAYAEEFGYS
                     SGIWGT"
     gene            133..261
                     /locus_tag="VNY06_06635"
                     /pseudo
     CDS             133..261
                     /locus_tag="VNY06_06635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020177494.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
     gene            354..1373
                     /locus_tag="VNY06_06640"
     CDS             354..1373
                     /locus_tag="VNY06_06640"
                     /inference="COORDINATES: protein motif:HMM:NF014542.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HWX14518.1"
                     /translation="MSICCQSTGVPHGRKNSRNRAHWLSRRGQDNASQSNSHGDIGID
                     NDLIVGADEEVFEMNNGCVCCTVRGDLIRIIEGLLKRRGKFDAILVETTGLADPAPVA
                     QTFFVDADVRDAARLDAVVTVADAKWLGARLKDAPEAKNQIAFADVILLNKIDLVSTD
                     EVNDIEARIRAINPYAKLHKTVNCALPLDAVLRRNAFDLERILDLEPEFLSDDDHHDH
                     GDHEHEHHGHDHKEPHGLKHYHDEEMQTVALSVDKDIDPEKFIPWLQDYVQQEGASIL
                     RLKGILAFENEPRRFVFQGIHMLFEGDLQREWEQGEKRQSKLVFIGRNLPKDTLRQGF
                     LACMV"
     gene            1395..2393
                     /locus_tag="VNY06_06645"
     CDS             1395..2393
                     /locus_tag="VNY06_06645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012592000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WD40 repeat domain-containing protein"
                     /protein_id="HWX14519.1"
                     /translation="MAAASLASNVTPIDAGVPVAAAGFLILVPALALDDGNVLLLTPQ
                     GAVRISAHPGASILVAAVTATQFFTGGDDGRVVTVREDGTIQEIAEEKGRWIDALAAK
                     EDGAIAWSSGKNVRARDASGNVKTSLAPSSVRGLAFMPKGYRIAFSHYDGASLWFPNT
                     ATPPDIFEWKGSHLSLAVSPDGRFLVTAMQENVLHGWRVADKTDMRMRGYPAKTRSLS
                     WSGDSHWLATSGADAAVIWPFKDKTGPIDKAPLECGARDVKVMQVAFHPKLPLLAQGY
                     EDGALLLCRIPDGAEIVVRDPPRGGRAISALAWDKSGRKLLFGTADGAAGLLDIPA"
     gene            2471..3263
                     /locus_tag="VNY06_06650"
                     /pseudo
     CDS             2471..3263
                     /locus_tag="VNY06_06650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003526010.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetate decarboxylase"
BASE COUNT          729 a          965 c          980 g          729 t
ORIGIN      
        1 gtgccccaga tgcccgagct gtaaccgaat tcctcggcat aggcccagag cgccacgacg
       61 ttgacaccgc gataggcctt cttcgatgcg atgttgacgg gccgcaggat gctcccggcg
      121 gcgcgatgcg cggaaaccgg agcgaagatt ggtggcgcgc tttattccga cgcgctttcg
      181 cctccggacg gaccggccgg aacctatatc gagatgaggc gcaacaatgt cagggaactt
      241 tccgctgcgc tgacgcaata gggcgaactt gacgggctcc ggcaagttta agacaaagca
      301 atagaaaagc tttctcgatt gcggcgtcct cgcgcgttgt tggaagaacg gcgttgtcga
      361 tttgctgcca atccaccgga gttccgcatg gccgaaaaaa ttcccgtaac cgtgctcact
      421 ggctatctcg gcgcgggcaa gacaacgctt ctcaatcgaa ttctcacggc gacatcggca
      481 tcgacaatga tcttatcgtc ggtgccgacg aagaagtctt tgagatgaac aatggctgcg
      541 tctgttgcac cgtgcgcggc gaccttattc ggatcatcga gggtctgctc aagcgccggg
      601 gcaaatttga cgcaatcctc gtcgagacca cagggcttgc cgaccctgcc ccagtggcgc
      661 agactttttt tgtcgatgcc gatgtacgtg atgctgcaag gctcgatgcg gtcgtaaccg
      721 ttgcggatgc caaatggctc ggtgcccggc tcaaagatgc accggaggcc aaaaaccaga
      781 tcgcatttgc cgatgtgatc ctcctcaaca agatcgatct cgtcagcaca gacgaagtca
      841 acgacatcga ggcgcgcatc cgcgcgataa acccctacgc caagctgcat aaaaccgtga
      901 attgcgccct gccattggac gcggtgctcc gccgcaatgc ttttgatttg gagcgcatcc
      961 tcgatctaga gcctgaattt cttagtgacg atgaccatca tgatcatggc gatcatgagc
     1021 atgagcatca tgggcacgat cacaaagagc cgcatggcct aaagcactat catgatgaag
     1081 agatgcaaac ggtggcgcta tccgttgaca aggacatcga tccggaaaaa ttcatcccat
     1141 ggctgcaaga ctatgtgcaa caggagggag cttcgatttt gcggctaaag ggcatacttg
     1201 catttgagaa cgagccaagg cgctttgtgt ttcaaggaat acacatgctt ttcgagggcg
     1261 acctccagcg cgagtgggaa caaggggaaa aacgccagag caaactcgtc ttcatcggcc
     1321 gcaacttgcc aaaagacaca cttcggcagg gatttttagc ctgcatggtg taacccacgc
     1381 aaaagcaaaa gaccatggcc gccgcctcgc ttgcctccaa tgtcacaccg attgatgcgg
     1441 gcgttcctgt cgccgcggcc ggctttctca ttcttgttcc agcgcttgcg ctggatgatg
     1501 gcaatgtgct gctcttaacg cctcaaggcg cggtgcgcat ctcagcgcac cccggcgctt
     1561 ccatcctcgt tgccgcagta actgcaacac aattctttac tggaggagac gatggccgcg
     1621 tggttaccgt cagggaggac gggacaatcc aagaaatcgc agaggaaaaa ggcagatgga
     1681 tcgatgcgct tgcagcaaag gaagatggtg cgatcgcttg gtcgagcggc aagaatgtgc
     1741 gtgcccgcga tgcgtccgga aacgtaaaga cctctctcgc accatcaagc gtgcgtggcc
     1801 ttgccttcat gccgaaaggc taccggatcg cattctccca ttatgatggc gcaagccttt
     1861 ggttcccaaa caccgcaacg cccccggaca tattcgaatg gaaggggtcg catctcagct
     1921 tggcggtctc tccggacggg cgttttctcg tgaccgccat gcaggaaaat gtgctgcacg
     1981 gctggcgggt ggccgataag acagatatgc gcatgcgcgg ctatccagca aagacccgct
     2041 ccttgtcctg gtcgggcgac agccattggc ttgcgacgtc gggcgctgac gctgcggtta
     2101 tatggccttt caaggataag accgggccga ttgacaaggc cccgcttgaa tgcggcgcac
     2161 gcgacgtgaa ggtcatgcag gtcgcattcc atcccaaact ccccttactg gcgcagggtt
     2221 atgaagacgg ggcgttgttg ctttgccgga tcccggatgg cgcagaaata gtcgtccgtg
     2281 atccgccgcg gggcgggcgc gcgattagcg cccttgcttg ggacaagagc ggtcgcaagc
     2341 ttctcttcgg aacagcagac ggcgcggctg gcctcctcga catacctgcc tgagagggtt
     2401 tttatttctg tcatcttgcg gcactagatt taggtctttg ccgggtttgc actcctgcag
     2461 gggccttacc atgaaaatag ccgatgttca ggcccgtgca ttcgcgatgc cgctcaataa
     2521 tccgtcctat ccgccgggac cttaccgttt ctatgaccgc gaatatttcg tcatcgaata
     2581 tctgaccgat ccgcaggcgc tgcgagccgc cgtgcccgaa ccgctcgaaa tttccggccc
     2641 agtcgtcaag ttcgaattca tttgcttgcc ggatacaacg ggctttggcg aattcaccga
     2701 atgtggccag atgatcccga tgcatttcga gggcaaaaat ggcatctttg ttcattcgat
     2761 gtttctcgac aatccttcgc caatcgcggc cgggcgcgaa atctgggggt ttccgcaact
     2821 tctggcgcgt gcaaaacttt gccacgaaag cgaggtgttg gtcgcatcgc tgcattttgg
     2881 ctcggtcctc tgcgccacgg gcagcatggg ctacaagcat aaaagcactc gaccctggcc
     2941 cggtgctccg ggagctcggc gagccgaatt ttctgattaa aatcattccg catgtcgatg
     3001 gcacgccacg catttgcgag cttgtgcgct atcactggca ggatattagg ctcaagggag
     3061 tctggggtgg accggcggcg cttcagcttt acagccatgc gatcgcaaat gtcgtgaggc
     3121 ttccggttct tgaagtgctc tcgggcactc attttgttgc cgacgtaacg ctcggtattg
     3181 gtgaagtcgt agaggactat ttggcacaag aaccgaaggc gcggctacca agcccttcct
     3241 tcgaggcgga ggctgcaaaa tgaacatcat gggtccgcca cgcgcgaagc cgctccggca
     3301 cgatgtcgcg gcgatcaccc aacgctacga tcgggtagcg ctcgtgctac aaggcggcgg
     3361 tgctctcggt gcctaccagg ttgcagtgta tcaggcgctc gcc
//
LOCUS       DATMPU010000292         3835 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_1477516,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000292 DATMPU010000000
VERSION     DATMPU010000292.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 3835)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3835)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3835
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1477516"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            198..464
                     /locus_tag="VNY06_06655"
     CDS             198..464
                     /locus_tag="VNY06_06655"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HWX14520.1"
                     /translation="MIDGSCDPIASRRFDEFFKEANISVEPVTLEQARLAREAYRDFG
                     KGSGHPAKLNFGDCFAYALAKTTGEPRLFKGDDFIHTDISSALG"
     gene            523..624
                     /locus_tag="VNY06_06660"
                     /pseudo
     CDS             523..624
                     /locus_tag="VNY06_06660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012454803.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            654..956
                     /locus_tag="VNY06_06665"
     CDS             654..956
                     /locus_tag="VNY06_06665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018409062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HWX14521.1"
                     /translation="MGQLRNPHPGEILKEEFLDKIGVSQNQLAQAIGVPGNRVHAIVK
                     GTRDITADTDLRLCKFFGLTDGYFLRLQNAYDMLEARRKIAREVTRIKRYKSDKAA"
     gene            1416..1664
                     /locus_tag="VNY06_06670"
     CDS             1416..1664
                     /locus_tag="VNY06_06670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX14522.1"
                     /translation="MADAAFDFGSRQSRSGKDRFFAKRTQFVVRTKEGHLGVGGVESI
                     VRMAMAPVFGEKKKLANSLVHLLTGGTQHRRHLEISGI"
     gene            1731..2033
                     /locus_tag="VNY06_06675"
     CDS             1731..2033
                     /locus_tag="VNY06_06675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011474646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HigB family
                     toxin"
                     /protein_id="HWX14523.1"
                     /translation="MRVIARNVLLAYARAHPETTIPLERWYRLVKAANWTSMDDIRRA
                     APKSKVLNRDRVRFEVVGGDYRLIAAFDFRRQIAFVKFIGTHAEYDAIDALTVARF"
     gene            2045..2434
                     /locus_tag="VNY06_06680"
     CDS             2045..2434
                     /locus_tag="VNY06_06680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013502019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HWX14524.1"
                     /translation="MQIRPIRTDKDHRAALAEIEKLWGASTGTPQGDKLDVLVTLVET
                     YEERRWSFKSRRRRFDPVDVLHYAIEELGHTQAELADILGSRSRASEVLARRRPLTLE
                     MIQKINAIWKIPADLLVQPYRVTAKAA"
     gene            3197..3451
                     /locus_tag="VNY06_06685"
     CDS             3197..3451
                     /locus_tag="VNY06_06685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015933983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapB family
                     antitoxin"
                     /protein_id="HWX14525.1"
                     /translation="MGLNIKNPETYRLVEELAKRTGESMTAAITIAVRERLDRIRGEQ
                     EAGLAERLLRIGQDCAKRLKEPYRSASHDKLLYDDRGLPR"
     gene            3448..>3835
                     /locus_tag="VNY06_06690"
     CDS             3448..>3835
                     /locus_tag="VNY06_06690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006724759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HWX14526.1"
                     /translation="MIIDTSAIIAILRDVAGAGDCALAIEKTRSRRISAANFVETALV
                     IDGSRDPIASRRFDEFFKEANISVEPVTLEQARLAREAYRDFGTGSGYPAKLNFGDCF
                     AYALAKTAGEPLLFKGDDFIHTDISSA"
BASE COUNT          841 a         1082 c         1120 g          792 t
ORIGIN      
        1 agcgcctcaa ggagccctac cgctcggcaa gccatgacaa actgctctat gatgatcgcg
       61 gcctgccccg atgattatcg atacctctgc gattatcgcg atcctgcgcg atgaagcggg
      121 cgccagcgac tgcgctggca attgagaaaa ctcggagccg ccgaatatct gcaactacct
      181 ttgttgaaac cgctctggtg atcgacggaa gctgcgatcc tattgccagt cggcgcttcg
      241 acgaattctt caaggaagcc aacatcagtg ttgagcccgt caccctggaa caggcccgcc
      301 tggcgcgcga ggcctatcgc gatttcggta agggaagtgg acaccctgca aaactgaatt
      361 tcggcgattg ctttgcgtat gccctggcaa agacaaccgg tgaacccagg ctcttcaaag
      421 gtgatgattt catccacacc gatatcagtt cagccttagg ctaaatgttt ggcgcctaca
      481 cgtcgacgat ttgtcttggc gaccgccggc cgagggcgta agcgagcggg ccagatgagt
      541 ataagaatca acgaccaatg gcgcatctgt tttcggtggg cggaaaagga cgccgaggat
      601 gttgagattg tggactatca ttaacccacg caaagatcag gaaggcaccg atcatgggac
      661 aacttcgcaa tccgcatccc ggcgaaattc tgaaagagga atttctcgac aaaatcggtg
      721 ttagccagaa ccaattggct caagcgattg gcgtgccggg caaccgcgtt cacgccattg
      781 tcaagggaac gcgcgatatt accgcagata cggacctgcg tctgtgtaag ttctttgggc
      841 tgacggatgg ttattttttg cgtcttcaga atgcctacga catgctggag gctaggcgca
      901 agattgcgcg cgaggtgacg aggatcaaac gctacaaatc ggacaaggcg gcataatctt
      961 ggacggacgc tctccgaacg taagcgaaat ccagtgattg cgagcgcgcg ctgcgtccgc
     1021 gctgggctct cgcatcacta catgtctcgc agcagattcc gcgcctctgc accaacgcct
     1081 ggacctgctt cgttcctcga tcgcgtgttt cgtaaccagg caatccgccg tgccttggag
     1141 tccgcgggca tcgagttcat cgacgagaac ggtggcggac ctggcgttcg gcttcggaaa
     1201 ccgccaaagc agaagcggtg agaaccggcg acgtctttgc gaaacgaacc caatgctttg
     1261 agctcttcag atcgcagcag tcggaaggcg aggaagctgc cgctagcatc gagtcgtcga
     1321 ccggaaaagc ggcggctgga ggtgggcaca gctagcgacg gaggccatcc gcagcatggc
     1381 caaggctgtc gacgccgagc tcatcgacga gaacggtggc ggacgcggcg ttcgacttcg
     1441 gaagccgcca aagcagaagc ggtaaggacc gctttttcgc gaaacgaacc caatttgtcg
     1501 tgcgaacgaa ggagggccac cttggagtcg gtggggtcga gtccatcgtg agaatggcga
     1561 tggcgccggt gttcggcgaa aaaaagaagc tcgcaaatag tttggtccac cttctgaccg
     1621 gtggaacaca acaccggcgt catctcgaga tttccggcat ttagagccat acgccttctc
     1681 gagagatttc gctcttgttc ccgtggtggg aacaatgtta tagttcatct atgcgggtga
     1741 ttgcgcgaaa cgttctgctg gcctatgccc gcgcgcatcc cgaaaccacg attccgcttg
     1801 agcgctggta caggctagtg aaggctgcga attggacttc gatggacgat attcgtcgtg
     1861 ctgcccccaa gtccaaagtg ctcaatcgtg atcgggtccg ttttgaggtt gttggcgggg
     1921 actatcggtt gatcgctgca ttcgacttcc gccggcaaat tgcgttcgtg aagtttatcg
     1981 gcacgcacgc tgaatacgat gccattgacg ctctgactgt cgctcgattt tgatgaggac
     2041 cgccatgcaa atccgtccga tccgcaccga caaagaccac cgggccgcac tcgccgagat
     2101 tgagaagctt tggggcgcgt ccactggaac accacagggc gataagctcg acgttctcgt
     2161 gacgctggtc gaaacgtatg aggagcggcg ttggtcgttc aagagccggc gccggcggtt
     2221 tgaccctgtc gatgtgctgc actatgcgat tgaggaactc ggtcacactc aggcggagct
     2281 cgccgatatt ctgggctctc gctcgcgcgc ctccgaggtc ttggctcggc gccggcccct
     2341 tacccttgag atgattcaaa agatcaatgc aatctggaaa atcccggccg atcttctcgt
     2401 gcaaccttat cgcgtcaccg caaaggctgc ctaagcaagt ttacgcagtc ttcatccacc
     2461 atcatttgat gtcccgccgc aacttccgcg acccctcaac ttgcttccgt tcctcgatcg
     2521 ccttgagggt gtgctgattt ttccatcggc gccggaccca gggtgacacg ccgcgcatcc
     2581 gacccagcct tttgtcgcgc accgacgtca gcgatcgaca agacacgccc aagcgacttg
     2641 gacccacgcg cgggctcgcc agcatcggcc cgctattgcg gggatgatcg ccggtcgaga
     2701 attcgacttt tagggtctgc cggaatggcc tcatcgacgg tggagatctg cccaggtctc
     2761 gaagtaggtg tagccgctat cgtcgggtag aaactattga gttggaaatc ccggtcactg
     2821 ctgcgaacga cgcggcgctt agtgccatct tggagttcgc gagccttgag ttcatcgagg
     2881 agaacggtgg cggacccggc gtacgccttc gaaagccgcc aaaacagaaa agatgagatt
     2941 tggcgccgtc ttcggaggag gcggccacag ctagcgacgc aggtcatctg cgaaagccgc
     3001 aaaagcgcag cgataaggac cgcggccgcc tttttgcgaa acgagcaaaa tctcctccgc
     3061 cgcgatcgaa gcgccgctta gagccacctt ggagtgcgag ggcgcttaga tcctgaaaac
     3121 caatctccgg tgattggccc gggccgtagt cgtctggtag attggtttct cgtacatggt
     3181 agaaaggaac caggttatgg gcctaaacat caaaaacccg gaaacctatc ggcttgttga
     3241 ggaactcgcc aagcgcacgg gtgagtcgat gactgcagca ataaccatag cggttcgcga
     3301 acggttggac cggattcgcg gcgaacagga agctggcctc gcggagcggc tgctgcggat
     3361 cggccaggac tgtgccaagc gcctcaagga gccctaccgc tcggcaagcc atgacaaact
     3421 gctctatgat gatcgcggcc tgccccgatg attatcgata cctctgcgat tatcgcgatc
     3481 ctgcgcgatg tagcgggcgc cggtgactgt gcgctggcaa ttgagaaaac tcggagccgc
     3541 cggatatctg cagctaactt tgttgaaacc gctctggtga tcgacggaag ccgcgatcct
     3601 attgccagtc ggcgcttcga cgaattcttc aaggaagcca acatcagtgt tgagcccgtc
     3661 accctggaac aggcccgcct cgcccgtgag gcctatcgcg atttcggtac gggaagtgga
     3721 taccctgcaa aactgaattt cggcgattgc tttgcgtacg ccctggcaaa gacagccggc
     3781 gaacccttgc tcttcaaagg tgatgatttc atccacaccg atatcagttc agcct
//
LOCUS       DATMPU010000293         3465 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_2375628,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000293 DATMPU010000000
VERSION     DATMPU010000293.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 3465)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3465)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3465
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2375628"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..290)
                     /locus_tag="VNY06_06695"
     CDS             complement(<1..290)
                     /locus_tag="VNY06_06695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HWX14527.1"
                     /translation="MQYWRDFVAGVVTRYHNDIKYWEIWNEFNGGGFAVNGTPQIYAD
                     LVRDAYDAAKKIDPTAKIGLNVANFDVGFLDRAIKVGAAGHFDYVAVHPYEI"
     gene            complement(360..761)
                     /locus_tag="VNY06_06700"
     CDS             complement(360..761)
                     /locus_tag="VNY06_06700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX14528.1"
                     /translation="MPAAQGPLERAVAALPSIGKLALIGFALSLAIIAISASRVHQAR
                     AESKSPVAATSSAQDVDAGPKDSRDDASPWGIATGAEWLGDYPKFNPLLNEAGVRWLR
                     GFPMWPSPSKANGIGQKQMRLSPMPAPTTFI"
     gene            840..1904
                     /gene="panB"
                     /locus_tag="VNY06_06705"
     CDS             840..1904
                     /gene="panB"
                     /locus_tag="VNY06_06705"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR00222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HWX14529.1"
                     /translation="MSARNLCTFVRDGEIQDKGFAKCCLRAQILCTGCAGEIATPWPP
                     QGQKPLSLSVNQFLRLSARIVNLLAANDRPKSDRRNEWDFMARRSHFRDMKRRDDPIA
                     VLTAYDAPTAKTEEAAGVDIILVGDSVGTAMLGYMSEREVTLADMRHHVGAVRRGAPK
                     TPIIADLPYHSYDTPESALNSSRLLIAAGADMVKFEGFLPDILAALVDASIEVCCHLG
                     LEPQHHEGKGVKGRKAAEASRLVRDAIALDKAGMAMLVLELMPEEVAGRVTKSVAAPT
                     IGIGAGRETDGQVLVITDVLGFTPTNYRHNRRYQEFGQIMLEAVSTYVFDVRSKKFPA
                     EANMFFMDRDELEKFLKDNP"
     gene            complement(1908..2357)
                     /locus_tag="VNY06_06710"
     CDS             complement(1908..2357)
                     /locus_tag="VNY06_06710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003612290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HWX14530.1"
                     /translation="MREKFSSELKIAMKAFDKRRVDTIRMIMAALKDKEIEARGQGKT
                     LSNEDILALLQKMTKSRKESQEIYEKAGRLDLSTQEAEEISVINSFLPQQLSGSEVEA
                     AIAAAIAETGASSVKDMGKVVGALKAQFAGRMDFATASARVKAILGA"
     gene            2441..>3465
                     /gene="carA"
                     /locus_tag="VNY06_06715"
     CDS             2441..>3465
                     /gene="carA"
                     /locus_tag="VNY06_06715"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012590640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HWX14531.1"
                     /translation="MTSRQEQAFPDWPGPRPTALLVLANGMVLEGFGLGATGSAVGEV
                     CFNTAMTGYQEILTDPSYSGQIVTFTFPHIGNTGVNDEDMETDNGAAHVGARGVVVHA
                     PITRPSSYRAKGDFNAWLKNRGIIGLCGLDTRALTAFIRENGMPNAAIANSPAGEFDI
                     DRLRAMARVWPGLEGMDLVPEVSSSRRYEWSHLTWVPGKGFPVAGPGTFRVVAIDYGI
                     KLNILRLLAEAGCAVTVVPAETRADSILAMRPDGVFLSNGPGDPAETGKYAVPVIQSL
                     LESGVPTFGICLGHQMLALAIGAKTLKMRQGHHGANHPVQDFTTGKVEIVSMNHGFAV
                     DPQTLPA"
BASE COUNT          753 a         1015 c          949 g          748 t
ORIGIN      
        1 aaaatctcat aagggtgaac cgccacatag tcgaaatgac cggcagcgcc gacttttatt
       61 gccctatcca aaaaacctac gtcaaaattg gcaacgttca ggccaatctt agcggttgga
      121 tcgattttct tggccgcatc atacgcatca cgcacgaggt ccgcataaat ctgcggcgtt
      181 ccgttgacgg caaacccccc gccattgaac tcattccaaa tttcccaata tttgatgtca
      241 ttgtggtagc gggtgactac ccccgcaacg aaatcacgcc aatattgcat gtcctttatc
      301 gggaacttac gcgaatctcc atgggcagac gcccagggcg agataggcaa gaggcgcggt
      361 caaatgaatg ttgttggcgc gggcattggc gacaagcgca tctgtttttg tccaattcca
      421 ttcgccttgc ttgggctcgg ccacataggg aagccgcgca gccatcttac gccagcctca
      481 ttgagcagag gattgaattt aggatagtcc cccaaccatt cggccccagt ggcaatcccc
      541 catgggctgg catcgtcgcg cgaatccttt gggccggcat cgacatcctg cgcagaactt
      601 gtcgcggcca ctggagactt gctttcggcg cgtgcctgat gaacgcgact tgctgaaata
      661 gcgatgatag caagtgacaa ggcaaagcca atgagggcaa gcttgccaat cgaaggaagc
      721 gcagcgacgg ctcgttccag cggcccttgt gcggccggca ctaaacccgc caatgccgcc
      781 ggatttgttg gccttatcat cgtcaatatt tcaacccgga agatgctctc gctttatgcg
      841 tgagtgctcg caatctttgt actttcgtcc gcgatggcga aattcaggat aaaggctttg
      901 caaaatgctg cctgcgtgcc cagattctct gtaccggatg cgccggagag atcgctaccc
      961 cctggccgcc acaggggcaa aagccccttt ccctgtcggt aaaccaattc ctgagactta
     1021 gcgcgcgaat tgtcaatctt ctggcggcca acgatagacc gaagtccgac cgccgcaatg
     1081 aatgggactt catggcacgc cgctcacatt tccgcgatat gaaaaggcga gatgatccta
     1141 tcgccgtgct gaccgcttat gacgcgccaa ccgccaagac tgaagaagcc gcgggcgttg
     1201 acataattct tgtcggcgac agtgtcggca cggcgatgct gggctatatg agcgagagag
     1261 aggtcacgct cgccgatatg cgccatcatg tcggcgccgt acggcgtggc gcacccaaaa
     1321 cgccaatcat cgccgatttg ccctatcata gctatgatac gccggagagc gcactcaaca
     1381 gctcccgtct cctcatcgcg gccggagccg acatggtgaa attcgaagga ttcctgccgg
     1441 acattctcgc cgcactcgtc gatgcttcga tcgaagtttg ctgtcacctg gggcttgagc
     1501 cgcagcacca cgagggcaag ggcgtcaagg gccgaaaagc cgcagaagcg tctcggctcg
     1561 tgcgcgatgc gattgctctc gacaaggccg gcatggcgat gctggtcctc gagctcatgc
     1621 cggaggaagt tgccgggcga gttacgaaat ccgttgccgc cccgaccatt ggaatcggcg
     1681 ctgggcgcga aaccgacggg caagtgctgg tgataacaga tgtactcggg tttacgccta
     1741 cgaactaccg ccacaaccgg cgataccaag aatttggcca gatcatgctg gaagccgtga
     1801 gcacctatgt cttcgatgtg cgttccaaga aatttccagc tgaggcaaat atgtttttca
     1861 tggacaggga tgagctagag aagtttctca aagacaatcc ctaaccctca cgcgccaagg
     1921 atcgccttca cgcgcgcgct ggcagtcgca aaatccatcc gccccgcaaa ttgcgccttc
     1981 agcgcaccga caacctttcc catgtctttg acggaggagg ccccggtctc ggcgatcgcc
     2041 gcggcgatcg ccgcctccac ttcggacccg ctcaattgtt gcggcaaaaa actgttgatg
     2101 actgaaatct cctcggcttc ctgcgtcgaa aggtcaagtc gcccagcctt ttcgtaaatc
     2161 tcttgcgatt ccttccggct ctttgtcatt ttctgcaaca gcgccaatat gtcttcgttg
     2221 gacagagttt tcccctggcc ccgcgcttca atttccttgt ccttaagcgc ggccatgatc
     2281 atgcgaatgg tgtcaacacg ccgcttgtca aaggccttca tcgcgatttt aagttcgctc
     2341 gaaaacttct cccgcattgg ataactcctg gccgcgaatt tgacggtgag cgtgcaccga
     2401 tttatcgaac tcgaactaca aggatggaat tagagtgcgc atgacaagca ggcaagagca
     2461 agcgtttcca gactggcccg gaccgcgccc aacggcgctc cttgttctgg ctaacggcat
     2521 ggtgctcgaa ggcttcggcc tcggcgccac gggctcggct gtgggcgaag tctgtttcaa
     2581 tacggcgatg accggctatc aagagatcct cacagatcct tcctatagcg gccagattgt
     2641 caccttcacc tttccccaca tcggcaatac cggggtcaat gatgaggata tggaaacgga
     2701 caatggcgca gcgcatgttg gcgcgcgggg ggtcgtcgtc cacgcgccca ttacgcggcc
     2761 gtctagctac cgggcgaagg gcgatttcaa cgcatggctc aagaatcgcg gcatcatcgg
     2821 cctttgcggc ctcgacacgc gagcgcttac cgcttttatc cgtgaaaatg gcatgccgaa
     2881 tgcggctatc gcgaattcgc cagctggcga attcgacatt gaccggcttc gggcgatggc
     2941 gcgagtttgg ccaggtctcg agggcatgga cctcgttcca gaggttagct cttcgcggcg
     3001 ctatgaatgg agccatttaa catgggtgcc cggtaaggga tttccggttg caggtccagg
     3061 aacatttagg gttgttgcga ttgactacgg gatcaagctg aatatcttac ggctccttgc
     3121 cgaggcaggt tgtgccgtga ctgtggtgcc ggccgagacc agggccgact caatcctcgc
     3181 gatgcggccc gatggagtct ttctctccaa cggtcctggc gaccccgccg aaactgggaa
     3241 atatgcagtc ccggtcatcc aatccctcct cgagagcggt gtgccgacat tcggaatttg
     3301 tcttggccat cagatgctgg cgctcgccat cggcgcaaaa acgctaaaaa tgcgccaagg
     3361 ccatcatggc gccaaccacc ccgttcagga ttttaccacc ggcaaagtcg agatcgtatc
     3421 aatgaatcac gggtttgcgg tcgatccgca gaccctgccg gcgaa
//
LOCUS       DATMPU010000294         8152 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_855889,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000294 DATMPU010000000
VERSION     DATMPU010000294.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 8152)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8152)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8152
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_855889"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1..244
                     /locus_tag="VNY06_06720"
                     /pseudo
     CDS             1..244
                     /locus_tag="VNY06_06720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012479022.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="integrase core domain-containing protein"
     gene            437..2767
                     /locus_tag="VNY06_06725"
     CDS             437..2767
                     /locus_tag="VNY06_06725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012385011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWX14532.1"
                     /translation="MARAETAHASARAGAMQGLATTAVQPALSRLANYEIWVRRAVPA
                     MVALFAGALAAITLISTHDAYDRAVTDAYAELELTAGVISSNLKEALRDSHGTDPAGA
                     LLQAAPSRALARGQQLVMTDATGNIAASLPGLSSSNSTLNDYLGPSQPLTDFAEKAGV
                     LRINLADGKDSLATVHTLDPPFGQVAIIYPLTAVLADWQAEAFRSAVLLFCTVLVLIA
                     LAIAYFWQASRARESNQLCERMQSRVDTALTRGRCGLWDWDLARGRIYWSDSMYEILG
                     MTSGDQFLSFGDINALVHPRDGDLTTMAEMLASSQVDSIDHAFRMQSARGDWIWLRAR
                     AELVRERASDSLHLVGIAVDITEQKALAERTATADLRLRDAIETVSEAFVLWDSTNRL
                     VMCNSKFQKFHHLPPDALLAGTPYAQIMGKGATPVIRTRIPLGERPVSGARTYEARFG
                     DGRWLQINERRTKDGGYVSVGTDITTLKRHEEQLMDSERRLVATVTDLRKSRQTLELQ
                     KGQLSILAAKAESASWAKSEFLANMSHELRTPLNAIINFAEMMHDETFGALGSPTYAE
                     YCKDIMNSGRYLLRVISDVLEMSQLESGRVQIQTEDFEIDTAVNAAVKSIGPIATEKE
                     IGLHAEPLPCREIHADRSAIEKILTILLRNAVKFTPNHGRITVHPRMVQNALNIYVAD
                     TGIGIPPAKLARLGRPFEQLDGILDDGMKGSGLGLAIARSLVDLHGGSMRIRSCVGSG
                     TIILVHLPQRREVARHKRKVGASILRQPAKPELLDA"
     gene            complement(2764..5712)
                     /locus_tag="VNY06_06730"
     CDS             complement(2764..5712)
                     /locus_tag="VNY06_06730"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012589242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008882 - [glutamate-ammonia-ligase]
                     adenylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamine synthetase]
                     adenylyltransferase/[glutamine
                     synthetase]-adenylyl-L-tyrosine phosphorylase"
                     /protein_id="HWX14533.1"
                     /translation="MNTDLPESGANLPLWQCLRTAPCLDDAPLAQSRLTEFLGMPGNA
                     RLQELADEPLVRNLLLALACHSPFLWRLATANSGRLMRCLETAPEACLKTCLSELAEA
                     AGPGSSETVVMRALRLAKQETALVIALADLGGVFDVVSTTEALSRVADNFISVALRCL
                     LRDAAVADRLRFPDCTNPEPGCGLVVLGLGKLGACELNYSSDVDIVVFFDPHCGAVAG
                     TVEPAKLFIRITKGLVRLLQERTGDGYVLRVDLRLRPDPGSTAVAISLPTAYSYYESL
                     GQNWERAALIKARPVAGDIALGETFLSNLTPFIWRKYFDYAAIADIAAMKRQIHAVRG
                     HEEIAVAGHDVKLGRGGIREIEFFVQTQQLIFGGKRPRLRGRRTLDMLDQLGKEGWIT
                     REAVADLRRSYLFLREIEHRLQMVADEQTQRLPQDQAALGRFANFCAYAHKTRFYSDV
                     THHLQLVSHHYGRLFEHSPRLDAAAGSLVFTGAGNDPETLETLLRLGFKNAPLAAETI
                     RGWHFGRRPAVRSPRAREILTELVPSLLQAFAYSGDPDAALAAFDTALAGTPAATELF
                     SVLKSNPPICQLFGEILGGAPRLARTVLSRPHLLDAAIDPNILKSALDEDAFRQRAGQ
                     SLKQQAQTEVFLDSVRDFAQEELFPIGLRLWSGMIEPFEAALAYSALASSVIDACFTH
                     LERVFAGEHGRIAHGRSIVLALGKLGSREMTAASDLDLLFIYDFDPSRPESDGARSLH
                     AVQYYTRFAQRLTSALTVATRRGRLYEVDMRLRPSGRQGPLATQFAHFADYQSKQAET
                     WERMALTRARAIVGDGPLMEQTRDFIDAVLRRPPGPALRSDVYAMRRLIAQSKGDEDP
                     WDLKLAAGGLIDIEFLAQYILLRDAHAEPGIASACASTVIERAAERGLLDKEAARLLG
                     SAYHLFTDVMQIQRLTLDPGANPREANEAVKRRLSKAGGQPTLSALESAIGELRTEVR
                     KIFERILSQG"
     gene            complement(5709..7163)
                     /locus_tag="VNY06_06735"
     CDS             complement(5709..7163)
                     /locus_tag="VNY06_06735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012589241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWX14534.1"
                     /translation="MALAKLFRTTVFKLSLAYLAISAIGAGIAISSVGWNMNHLINEQ
                     IAQTIDTDIAGLSEQYTQGGLRRLVNIIQKRTRRPGARLYLVTTPAGEPIAGNIMRLP
                     PAILESTGLVETDYQPAGDTRGKHRALSRVFVLAGGFHLLVGHDLEEGKQLRQILGQT
                     LLTSLLWLVMTGTLGGLWIARRVLMRVDAINANAQAIVVGDLSGRLRLAGTGDEFDRL
                     VQNFNAMLDRIGILMAGLKEVTDNIAHDLKTPLTRLRAGMEQALRSAKGSDEYRSALE
                     KALDESERLIQIFDALLSIARAEAGAGREGMSDFDAGLVVQDLGDLYEPVAEQRGVLF
                     SVSAEPELYLHGSRELVSQAVANLIDNGLKYGTADTSARGPDRAATAIKLAVRRSGRN
                     IEFIVADHGPGIAEKDRQRVLDRFVRLENARSRPGSGLGLSLAAAVARLHNGTLRIED
                     NRPGLRAVISLPAAPLRDAQPRLTLAFPRSDEAA"
     gene            complement(7150..7833)
                     /locus_tag="VNY06_06740"
     CDS             complement(7150..7833)
                     /locus_tag="VNY06_06740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018265704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HWX14535.1"
                     /translation="MRILLVEDDTEAASYMVKAFREAGHVADHVGDGLEGLARARDET
                     YDVLIIDRMLPKLDGLTLIGTLRAQKIVTPALILSALGQVDDRVKGLRAGGDDYLVKP
                     YAFLELLARVEVLARRNWSGSGEETIYRVNGLELDRLAHKLTRDGKEIPLQPREFRLL
                     EYLMKNAGQVVTRTMLLENVWDYHFDPQTNVIDVHISRLRAKIDKGFDPPLLHTIRGA
                     GYMIRDGSC"
     gene            complement(7966..>8152)
                     /locus_tag="VNY06_06745"
     CDS             complement(7966..>8152)
                     /locus_tag="VNY06_06745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012589239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HWX14536.1"
                     /translation="AQKGMQVGDVILEAGGRAVSRPADVAAALAEAKKDNRKAVLLRV
                     KSGDGTHFVAIAVNPAS"
BASE COUNT         1789 a         2450 c         2319 g         1594 t
ORIGIN      
        1 cttaaaagcc ttcggcctgc gccaaatctt cacccgtccc tatacgccaa agaccaaggg
       61 aaaagccgaa cgcttcatcc agacatccct gcgagaatgg gcctatgcga tggcttacga
      121 aaactccctc cagcgccaag catcaattgg ccgcctggct gcaccgctac aactggcacc
      181 gcccaaaccg cccgtcactc gcctcggcct ttcaatggac aacctgttga ggctccacag
      241 ctagcggcat gggcccggtt tctcttttct cttgccctgc ttacgccaag gggcccagtg
      301 agacaccttc agcgcggcga tcaaatttgc gttcatggtt cattaacctc tggacaaatc
      361 gaaaaactga gactcagatg gagctgattc ggagagatgc ggcacatcgc ttcgatgagc
      421 ttcgaggggg ccagacatgg cgcgcgcaga gactgcgcac gcttccgcgc gagcgggagc
      481 aatgcagggg cttgccacca cggcggtgca gcccgcgcta tcacgcttag ccaattacga
      541 aatttgggtg cggcgcgcgg tccccgccat ggtggcgctg tttgctggag cgcttgctgc
      601 aatcaccctt atttcgaccc acgatgccta tgaccgggcg gtaacggacg cttatgcgga
      661 gcttgaacta accgctggcg tcatctcaag caatctcaaa gaagcgctgc gggattccca
      721 tggaacggat ccggcgggag ctttgttgca agcggcgccc tcccgcgctc tggcgcgcgg
      781 gcagcaactc gtcatgaccg acgccacggg aaatatcgcc gcctccttgc ccggcctatc
      841 cagttccaac agcacactta acgattattt ggggccatca cagccgttga cggattttgc
      901 cgaaaaggcc ggtgtgttgc gcatcaatct tgccgatggc aaagactcgc tcgcaactgt
      961 gcacacgcta gatcctccct ttggccaagt cgcaatcatt tatccgttga ctgccgtcct
     1021 cgccgattgg caagcggagg cgtttcgttc cgccgtcctc ttgttttgca cagtccttgt
     1081 cctgatcgcc ctcgccatag cctacttttg gcaggcctcg cgtgcccgag agtccaatca
     1141 attgtgcgaa aggatgcaga gccgcgtcga tacggcactc accagaggcc gttgcggtct
     1201 ttgggattgg gacctcgcgc gcgggcgcat ctattggtcg gactcgatgt atgaaatcct
     1261 cggcatgact tcgggcgacc agttcctttc gtttggcgac ataaacgccc tggttcatcc
     1321 gcgggacggc gatctcacga cgatggctga gatgctggca agttcgcaag tcgattcgat
     1381 cgaccacgct ttccgaatgc agagcgcaag gggcgactgg atttggttgc gggcacgggc
     1441 agagctcgtc cgggaacggg cgagcgactc tctccacctt gttggcatcg cagtcgacat
     1501 caccgagcaa aaagcgctgg ccgagcgcac cgccacggcc gacctcagac ttagggacgc
     1561 gatcgagacc gtgtccgaag ccttcgtgct ttgggactcc accaaccgcc tcgtcatgtg
     1621 caattccaaa ttccagaaat tccatcacct gccgccggat gcgctcctag ccggaacgcc
     1681 ctatgcgcaa atcatgggaa agggtgccac tccggtcatc cggacgcgaa taccgctcgg
     1741 cgagcggccg gtttccggag cccgtactta tgaggcgcgc tttggcgacg gccgctggct
     1801 gcaaatcaat gaaaggcgca caaaggacgg cggctatgtc tcagtcggca ccgatattac
     1861 cacactcaag cgccacgagg aacaattgat ggactcagag cgccgtctgg tggcaacagt
     1921 tacggatttg cgcaaatctc ggcaaactct cgagctgcag aaaggccaac tttccattct
     1981 cgcagcaaag gctgaatccg cgagttgggc taagtcagaa tttcttgcca atatgagtca
     2041 cgagcttcgc actccgctca acgcaatcat caacttcgca gaaatgatgc atgatgagac
     2101 atttggcgcc ttaggatcac cgacatatgc cgaatattgc aaagacatca tgaatagcgg
     2161 ccgatatctg cttcgcgtga tctcggatgt tctggagatg tcgcagctcg aatccggccg
     2221 ggtccagatt cagactgaag atttcgagat cgacaccgcc gtcaacgccg cggtcaagtc
     2281 tatcggaccg atcgccaccg aaaaggaaat cggcctacac gctgaaccct tgccgtgtag
     2341 agaaattcat gccgatcgca gcgcaatcga gaagatactc acgatcctat tgcggaacgc
     2401 ggtgaaattc acgcccaacc acggccgcat cacggttcac ccccggatgg tccagaacgc
     2461 cctaaatatt tacgttgcgg ataccggtat cggaattccg cccgcaaagc tcgcaaggct
     2521 tggccgtccc ttcgaacaat tggacggcat actcgacgat gggatgaagg gctcaggtct
     2581 tggccttgcc atagctcgtt cgcttgtcga tctgcatgga ggctcgatgc ggattcgctc
     2641 ctgtgttggc agtggcacga taatccttgt tcatttgccg caaaggcgcg aggtcgcccg
     2701 acacaagcgg aaggtggggg caagtatatt gcggcagcct gcaaagccgg aactgctaga
     2761 cgcctaaccc tgcgacaata tacgctcgaa aattttgcga acttccgttc gtagctcgcc
     2821 aatcgcgctt tcaagcgcac ttaatgttgg ctgaccccca gctttcgaaa gacgccgctt
     2881 cacagcctca ttcgcctcgc gagggttggc gccgggatcg agcgtcaggc gctgaatctg
     2941 catgacgtct gtaaagaggt ggtaggcgct ccctaataag cgggcagctt ccttatcaag
     3001 caatccgcgc tccgcggcgc gctcgatcac cgtcgaagcg caagcgctag caatgccggg
     3061 ctcggcatgg gcatcgcgaa gcagaatata ctgggccagg aactcaatat cgatgagtcc
     3121 ccctgccgcc aacttaagat cccacggatc ttcgtctcct tttgactgcg cgatcaagcg
     3181 ccgcatggcg tagacgtcgc tgcgcaacgc gggcccggga ggccgcctca gcacggcatc
     3241 aatgaaatct ctggtttgct ccattaaggg gccgtctccc acaatggccc gtgctctggt
     3301 caaggccatg cgctcccagg tttccgcctg cttgctctga tagtcggcga aatgcgcgaa
     3361 ttgggttgca agtggcccct gccggccgga agggcgcagc cgcatatcga cctcataaag
     3421 cctcccgcgc cgcgtcgcga cggtgagagc ggaggtgagg cgctgggcga agcgcgtata
     3481 atattgcacc gcgtgcagtg aacgcgcccc atctgactca ggccgcgacg gatcgaagtc
     3541 ataaatgaac aagagatcga gatcggaagc tgctgtcatt tcgcgcgagc caagcttgcc
     3601 gagcgccaag acgatcgacc tgccatgagc aatgcgccca tgttcgcccg caaagactcg
     3661 ctccagatgc gtaaagcaag cgtcaatgac gcttgaagca agagccgaat aagcgagcgc
     3721 cgcctcgaaa ggttcgatca ttccagacca caaccgcaat ccgattggaa agagctcttc
     3781 ctgcgcgaaa tcgcgcacag aatccaggaa tacctcggtt tgagcttgct gtttcaagga
     3841 ttgcccggcg cgttggcgga acgcatcctc atcgagagca gacttgagaa tatttggatc
     3901 gatcgcggca tcaagcagat gcgggcggct taggaccgtg cgtgcgagtc gcggcgcgcc
     3961 gcccagaatt tcaccaaaca attggcaaat cggcggattg gactttaaca cagaaaagag
     4021 ttcggtcgct gcgggcgtgc ccgccaatgc ggtatcaaat gcggcgagcg cagcgtcggg
     4081 atctcccgaa taggcaaatg cttgcaagag cgagggcacg agctcggtga ggatttcccg
     4141 cgcacgcggg cttcgtaccg ccggacggcg gccgaaatgc cagccccgga tcgtctcggc
     4201 agcgaggggc gcattcttaa aaccgaggcg cagcagtgtt tcgagagttt ccgggtcgtt
     4261 tccagcaccc gtgaaaacaa gactgccagc cgccgcatcg agccgcggcg aatgctcaaa
     4321 cagccgtccg taatgatgtg agacaagctg caaatggtgg gtcacatcgc tgtaaaaccg
     4381 ggttttgtgc gcgtaagcgc aaaaatttgc gaagcgtcca agcgccgcct gatcttgagg
     4441 aaggcgttgc gtttgctcat cggcgaccat ttgcagccgg tgctcaattt cgcgcaaaaa
     4501 gagataggac cggcgcaagt ccgccaccgc ctcgcgcgtg atccagcctt ccttgccgag
     4561 ttgatcgagc atgtcgagcg tgcggcggcc ccgcaggcgc ggtctcttgc cgccaaagat
     4621 caattgctgg gtctggacaa aaaattcgat ctcccgaata ccgccgcgcc ccagtttcac
     4681 atcgtgaccg gccacagcaa tttcttcgtg gccgcgaacc gcgtgaattt ggcgcttcat
     4741 cgcagcaatg tctgcgatcg cggcgtagtc aaaatatttc cgccagatga aaggcgtgag
     4801 attggaaaga aacgtctcac cgagcgcgat atcgcctgct accggccgcg ctttgatcaa
     4861 agccgcgcgc tcccagttct ggccaaggct ttcataatag gagtaagcag tcggaagcga
     4921 aattgctacc gccgtcgagc cggggtcggg gcgtagccgc aaatcgaccc gcaagacata
     4981 gccgtcgccc gtccgctcct gaagcaaccg gacgagaccc ttggtgatgc ggataaagag
     5041 ctttgcaggc tccaccgttc cagcaaccgc cccgcaatgg ggatcgaaga aaacaacaat
     5101 atcgacgtcg ctcgaataat tgagttcgca cgcgccaagc tttccaagcc ccaagacaac
     5161 aagaccgcag cccggttcgg ggttggtgca atcgggaaac cggagccgat ccgcgacggc
     5221 ggcatcgcgc aataggcagc gaagcgccac cgatataaaa ttatcggcaa cgcgcgaaag
     5281 cgcctccgtc gtggaaacaa cgtcgaagac gccgccgaga tcggcgagcg caatgacgag
     5341 agcggtctcc tgctttgcaa ggcgcagtgc gcgcatgacg acggtttcgc tcgatcccgg
     5401 accggcggct tccgctaatt cggaaagaca tgtcttaaga caggcctcgg gcgccgtctc
     5461 aagacatcgc ataagccttc cggaatttgc tgttgcaaga cgccagagaa agggcgaatg
     5521 acaggctagc gccagaagaa gattgcgcac tagcggctca tcagcgagct cctgcagtcg
     5581 cgcattgcca ggcattccca ggaactcagt aaggcgagat tgggcgagtg gcgcatcgtc
     5641 aagacaaggc gcggtgcgca agcactgcca aagcggcaaa ttcgctccgc tttctggaag
     5701 atccgtgttc acgccgcctc gtccgaccta gggaaagcga gcgtcagccg cggctgtgcg
     5761 tcgcgcaacg gagccgccgg aagagaaata acagcacgaa gtcctggcct gttatcttcg
     5821 attcgcaagg ttccattgtg gagacgcgcc accgccgccg cgagggatag gccaaggcca
     5881 gagccgggcc gcgagcgagc gttttcaagc cggacaaatc gatcgagcac acgctgacga
     5941 tctttttctg caatgcccgg accgtgatcc gctacgataa attcgatatt tctgccgctg
     6001 cgccgcaccg cgagtttgat ggccgtcgca gcccgatccg ggccacgcgc agaggtatcc
     6061 gcagtaccat atttcagccc attgtcgatg agatttgcaa cagcctgact gacaagctca
     6121 cggcttccat gtaagtagag ctccggctcg gcggagaccg aaaacaatac gccgcgttgt
     6181 tccgcaaccg gctcgtagag atctcccagg tcttgtacga ccaacccggc atcaaaatca
     6241 ctcatgcctt cgcgcccggc acctgcctct gcacgggcga tgctcaacag agcgtcaaag
     6301 atttggatca gccgttcgga ctcatcgagc gccttttcga gggcggagcg atactcgtca
     6361 gagcctttgg cagagcgtaa tgcctgctcc atgcccgcgc gtaaccgggt caacggtgtc
     6421 ttgagatcat gggcgatgtt gtcggtaacc tctttcaatc ccgccatcaa tatgccgatc
     6481 cgatccagca tggcgttgaa attctgcacc agccggtcaa attcatcgcc ggtcccagcc
     6541 aagcgcagcc gcccagagag gtcgccgacg acgattgcct gagcattggc attgatagca
     6601 tcgacacgca ttaacacgcg ccttgcaatc cacagccctc cgagcgtgcc ggtcatcacc
     6661 agccagagca gtgatgtgag aagtgtctgg ccaagaattt ggcgtagttg tttaccttcc
     6721 tcgaggtcgt ggccgacgag cagatggaag ccgcctgcca acacaaaaac acgcgagaga
     6781 gcccggtgtt ttccgcgtgt atcacccgcg ggctggtagt ccgtctcgac taggccggta
     6841 ctttccaaga tggctggcgg cagtctcatg atgttaccgg cgatcggctc gcccgccgga
     6901 gtggttacaa gataaagacg ggcgccaggc cggcgggtcc gtttttggat gatgttcaca
     6961 agccggcgca gtccgccttg cgtatattgt tccgaaagcc cggcgatatc cgtatcaatg
     7021 gtctgcgcaa tctgttcatt gatgagatgg ttcatgttcc agccgacact gctgatcgca
     7081 attccggcac cgatcgcgga aatcgccaga taggcgagcg atagtttgaa gactgtggtg
     7141 cgaaagagct tagcaagagc catcgcgtat catatatcca gcaccgcgta tcgtgtggag
     7201 gagcggtggg tcaaaacctt tgtcgatctt ggcgcgcagc cgcgaaatat gaacgtcgat
     7261 gacattggtt tgcgggtcaa aatgataatc ccaaacgttt tccaagagca ttgtacgcgt
     7321 gacgacctgc cctgcatttt tcatcaaata ttcgagcaag cggaattcac gcggctgcag
     7381 aggaatttcc ttgccatcgc gggtgagttt gtgagcaagc cggtcaagtt ccaggccgtt
     7441 aacccgatag atcgtttcct cgcctgagcc agaccagttg cgccgcgcga gcacttcgac
     7501 ccgcgccagg agctccaaaa atgcataagg cttgacgaga taatcgtccc cgcccgcacg
     7561 caaccccttg acccgatcgt ccacctgccc aagcgctgac agaatgagcg cgggcgtgac
     7621 gattttttgc gcgcgcaggg tgccaatgag cgtcagccca tcgagcttcg gcaacatccg
     7681 gtcgatgatc aaaacgtcat aggtttcgtc ccgggctcgc gccaagccct cgaggccatc
     7741 gccgacatgg tcggcgacgt gcccggcctc gcgaaatgcc ttgaccatat agcttgccgc
     7801 ttcagtgtcg tcttcgacaa ggagaattcg catggccaag acttataacc catccacgca
     7861 aaaaggcagc aggccggaag ttaccttccg gcctgtcgcc ttcttaagcc ggcagcgcta
     7921 cgggggggac gcaccggctt tgtccgagcc tcggggacgt gaggctcagg aagctggatt
     7981 gacagcgatt gcgacgaaat gcgtgccgtc accgctcttg acccgcaaca ggaccgcctt
     8041 gcgattgtcc ttcttcgcct cggccagcgc cgcggcaacg tccgcgggtc tgctgaccgc
     8101 acggccgccc gcctccaaga tcacgtcgcc tacctgcatg cccttttggg cg
//
LOCUS       DATMPU010000295         3807 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_2100875,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000295 DATMPU010000000
VERSION     DATMPU010000295.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 3807)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3807)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3807
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2100875"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(32..625)
                     /gene="hisB"
                     /locus_tag="VNY06_06750"
     CDS             complement(32..625)
                     /gene="hisB"
                     /locus_tag="VNY06_06750"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012591547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HWX14537.1"
                     /translation="MRKGAISRKTKETDIEVRVDLDGAGLANIATGIGFFDHMLEQLA
                     RHSLIDIEIRAEGDLHIDQHHTVEDTGIALGQALRQALGGMEGIVRFSDVHLPMDEAL
                     TRVVVDISGRPFLVFKTKFAQPKIGSFDSELVREFFQAFATHAAMSIHVETLYGDNAH
                     HIAESAFKGLARALRAAVAVDQREKGAIPSTKGLLSC"
     gene            1348..1920
                     /gene="hslV"
                     /locus_tag="VNY06_06755"
     CDS             1348..1920
                     /gene="hslV"
                     /locus_tag="VNY06_06755"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013217299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HWX14538.1"
                     /translation="MQDHFSGREAWHATTIVMVKKGGRTVIGGDGQVSVGQTIIKGNA
                     KKVRRLAKGEVIGGFAGATADAFTLFERLEMKLEQYPGQLMRACVELAKDWRTDRYLR
                     RLEAMMLVADAQAALVLTGTGDVLEPETSDDGSVMGIGSGGNYALAAGRALLSTDLGA
                     EAIARRALEIAAGICVYTNDNFLLESIESI"
     gene            1945..3252
                     /gene="hslU"
                     /locus_tag="VNY06_06760"
     CDS             1945..3252
                     /gene="hslU"
                     /locus_tag="VNY06_06760"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011500844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HWX14539.1"
                     /translation="MTDFSPREIVSELDRFIVGQKDAKRAVAIALRNRWRRLRLEGTM
                     REEVLPKNILMIGPTGCGKTEISRRLAKLAGAPFLKVEATKFTEVGYVGRDVEQIVRD
                     LLETAITMMKEEKRKAVHARAQLAAEERVLDALVGTTASASTRDTFRRKLRSGELDDK
                     EIEIEIAQPSSGVPMFELPNMPGSAIGAISIGDIFGKALGGRAKPRRMMVKDAHAPLI
                     VEESDKMIDQDQIIQEAIREVENNGIVFLDEIDKICVREGRLGGDVSREGVQRDLLPL
                     IEGTTVSTKHGTVKTDHILFIASGAFHMSKPSDLLPELQGRLPIRVELSPLNEDDFRR
                     ILTETEASLIKQYEALLRTEGVELVFTPEAINAVAKIAVQVNSAVENIGARRLQTVME
                     RVLDEVSFTASDRFSERIEIDAAFVEKNIGDLARNADLSRFIL"
     gene            3327..>3807
                     /gene="trpS"
                     /locus_tag="VNY06_06765"
     CDS             3327..>3807
                     /gene="trpS"
                     /locus_tag="VNY06_06765"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012591560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HWX14540.1"
                     /translation="MSKFIERVFSGVQPTGNLHLGNYLGAIVKFVELQKTHDCIYCVV
                     DLHAITLPQKPEELRASIREVTAAFIACGIDPEKHIVFNQSQVAEHAELAWVFNCVAR
                     IGWLNRMTQFKEKAGKDRENASVGLYAYPCLMAADILAYRATHVPVGEDQKQHLELAR
                     "
BASE COUNT          820 a         1111 c         1066 g          810 t
ORIGIN      
        1 caaaaaaact gcttctttta gggaccttgc attagcagga aagcaagcct ttggtggagg
       61 ggatcgcacc cttttcacgc tggtcgaccg caaccgccgc gcgcaacgct cgggccaggc
      121 ccttgaacgc agactccgcg atatgatgcg cattgtcgcc gtacagcgtc tcgacgtgga
      181 tgctcatcgc ggcgtgggta gcaaaagcct ggaaaaactc gcgcacgagt tcgctatcga
      241 aactgccaat cttcggctgc gcgaatttcg tcttaaaaac gagaaacggc cgcccggaaa
      301 tatccaccac gacccgcgtc agagcctcat ccatcggaag atgcacatcc gaaaaacgca
      361 caataccttc catgccgccg agagcctggc gcagcgcctg accgagagct atgcccgtgt
      421 cctcgacggt atggtgctga tcgatgtgaa gatctccctc ggcacggatt tcgatgtcga
      481 tcagcgagtg gcgggcaagc tgctcaagca tatggtcaaa aaagccgata cccgtcgcga
      541 tattggcaag gccagcccca tcaagatcaa cccgaacctc gatatcggtt tccttggtct
      601 tgcgtgagat ggcgcctttg cgcatgttcg aactttctcg agttctttga gcccggcttc
      661 tagcaagtcc ttcgcggctt ggctatcctt ggcaagttgg ggtttgcgtg ccgtccctcg
      721 tcctggtcgc ggtccttggg ttgacgacgg cgaacccgac tgccgtcggc atggaagcgc
      781 ttgcaacgca ttcgcccgtt ggcgcgcaac acgccatgcg ctcctgaaca gcatcgcttt
      841 tttgcgctca ttcatctcat agccaatggc gatatcgcct ccttcacctt tttggcgcca
      901 tcgaggtcac ggcgcttgga ggaaccgttt cgatcgacgc caagcggcgt cgcgctctcg
      961 gtcctgcctg gcaatccttc gcgacgcaaa cttcgattct cctccctgcg gcgatcgcca
     1021 tcggacgcat aacgctgaac ttggttaaat cgtgcttggc gatgggccgc tgctattcta
     1081 atctatgtcc tgtttccagg ccgccattcg cacgcctttg gcgtctcgcc ctttcaggga
     1141 tgaaactccc atattggcct aacccaccca atcactgcgg gacccaaaag ggggcagcgg
     1201 cctttttgtc aggcccgcgt ccacttagct ctcccttctg ccggcgcctc cgcacttgca
     1261 tcccttcgta caagcccata gatgaaaatc gctaaacctt accctacccc gaaaccatgg
     1321 cgctgggcga cttgtgagag tattttgatg caggatcatt tctccggacg tgaggcttgg
     1381 cacgcgacca caattgtcat ggtcaagaag ggcggccgca cggtcatcgg cggtgacggc
     1441 caggtctccg tggggcagac gatcattaaa ggcaatgcaa aaaaggtccg ccggctcgcc
     1501 aagggcgaag tgataggcgg ctttgccgga gcaaccgccg atgctttcac cctgttcgag
     1561 cgtctggaga tgaagctcga acaatatccg ggacagctca tgcgcgcctg cgtcgagcta
     1621 gccaaggatt ggcgcactga ccgctatttg cgccggcttg aggcgatgat gcttgttgcc
     1681 gacgcgcagg cagcattggt cctgacaggc accggcgatg tgctcgaacc ggagacgagc
     1741 gacgatggca gcgtgatggg aataggctca ggcggcaatt acgcactcgc cgcgggccgc
     1801 gctttgcttt cgaccgacct cggtgccgag gcgattgccc gccgcgcgct tgaaatcgcc
     1861 gctgggattt gcgtttatac caacgacaat tttctgctcg agtcgattga atcgatatga
     1921 ccacgcggcg acaggaaaac ctagatgacc gatttttcac cgcgtgagat cgtctccgag
     1981 ctcgaccgtt ttatcgtcgg gcaaaaagat gccaagcgag ccgtggctat cgctttgcgc
     2041 aaccgctggc gccgccttcg cctagaaggc acgatgcgag aagaggtgtt gcccaaaaat
     2101 attttgatga ttggaccgac cggctgtggc aagaccgaaa tttcgcgccg cctcgccaag
     2161 ctcgcgggcg caccattctt gaaagtcgag gcaaccaaat tcactgaggt cggttatgta
     2221 ggtcgcgatg tcgagcagat cgtccgcgat cttcttgaga ccgcgatcac catgatgaaa
     2281 gaggaaaagc gcaaggcggt gcatgcccgc gcgcagctcg ccgcggaaga gcgcgtcttg
     2341 gatgctcttg tcggaaccac cgcctcggcc tcgacccgcg acacttttcg cagaaagcta
     2401 cggtcgggcg aactcgatga caaggaaatc gaaatcgaga tcgcccagcc ttcgtccggg
     2461 gttccgatgt tcgagctccc aaatatgccg ggctccgcta ttggcgcaat ttcgatcggc
     2521 gacattttcg gcaaggccct tggcgggcgt gcaaagccgc gccgcatgat ggtgaaggac
     2581 gcgcatgctc cgctcattgt cgaggaaagc gacaaaatga tcgatcagga tcaaatcatc
     2641 caggaggcga tccgcgaggt agaaaacaat gggatcgtgt ttctcgatga gatcgacaag
     2701 atttgcgtgc gcgaagggcg tcttggcggc gatgtctcgc gcgagggcgt gcagcgcgat
     2761 ctcctgccgc tcatcgaagg caccacggtg tcgaccaaac acggaacggt caaaaccgac
     2821 catatcttgt tcatcgcatc cggcgccttc catatgtcaa aaccatcgga cctgttgccc
     2881 gaattgcaag gcaggctgcc gataagagtc gagctctcgc ctttgaacga ggacgatttc
     2941 cgccgtatcc tcaccgagac ggaagcaagc ctcattaagc aatatgaagc gctgcttcgc
     3001 accgagggag tcgagcttgt gtttacacca gaggccatca atgcggtggc gaagatcgct
     3061 gtgcaagtga attccgccgt cgagaatatt ggcgcgcgac gactccagac tgtgatggaa
     3121 cgcgtcctcg acgaggtaag cttcaccgct tccgatcgtt ttagcgagcg catcgaaatc
     3181 gacgctgcct tcgttgagaa aaatataggt gatcttgcca ggaacgccga tttgagccgg
     3241 ttcattcttt gaccttcggg acaaccgttc gcatgggttc cccagctctc ttattttcgg
     3301 acagtagcgt cagggacacc tctgctgtgt caaaattcat tgaacgggta ttttccggcg
     3361 tccagccgac cggcaatttg catcttggaa attatctcgg cgcgatcgtc aaattcgtcg
     3421 agctgcaaaa gacacacgac tgcatttatt gtgtcgtcga tctccacgcc atcaccctgc
     3481 cgcaaaagcc cgaagaattg cgggcaagca tcagggaagt aacagcggca tttatagcct
     3541 gcggcatcga tcctgaaaaa cacatcgtct tcaaccagag ccaagtcgct gaacatgccg
     3601 agcttgcctg ggtgtttaat tgcgtggcgc ggatcggctg gctcaaccgc atgacccaat
     3661 ttaaggaaaa ggccggcaag gaccgggaga atgcgtcggt cgggctttat gcctatccgt
     3721 gcctcatggc ggccgatatt cttgcctatc gtgcaaccca tgtcccggtt ggcgaggacc
     3781 aaaagcagca tctcgaactt gcccgcg
//
LOCUS       DATMPU010000296         3825 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_3946556,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000296 DATMPU010000000
VERSION     DATMPU010000296.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 3825)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3825)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3825
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3946556"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3072
                     /locus_tag="VNY06_06770"
     CDS             <1..3072
                     /locus_tag="VNY06_06770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014892317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate ferredoxin oxidoreductase family
                     protein"
                     /protein_id="HWX14541.1"
                     /translation="GTSKYGGVLALMGDDHTAESSTTAHQSEFNFVNVMIPVLSPAGV
                     QEIVDYGLYGYAMSRFTSAWVGLKCLKDTVESTGSIDVSLDRVRPVIPAGFIMPEGGL
                     NIRTGDTVLDQEKRLQNYKRDAMLAWLRANQLNKIITSGGAHAKLGVITAGKSYLDVR
                     QAMDDLGIDEIAANRLGLRILKLACTWPIEPQGLHEFARGLELIIIVEEKRSLIEVQL
                     REELYGSPSQPVCVGKKDEEGNWLFPVTGSLDANDIAIAIGRRLLRFNQSEELENRVR
                     QLERAQAVLAAAGDLGVRIPYFCSGCPHNSSTRIPDGMRAYAGIGCHYMVQWMDRSTE
                     GFTQMGGEGTNWIGEAPFSKRSHVFQNLGDGTYDHSGILAIRFAVASGVNITFKILFN
                     DAVAMTGGQPLEGGLTVEMVARQVAAENVKRIVVVTDEPKKYPASTRWPEGLTVKDRH
                     EFDSVQRELAAIPGVTVLIYDQTCAAEKRRRRKKKLMPDPDRRVLINELLCEGCGDCG
                     AKSNCVSIQPLETEFGRKRIIDQSSCNKDFSCLDGFCPALVTVHGARPRKAKVKEQPD
                     FPELPRPVIPAIGAKPYGILVTGVGGTGVVTIGAILGMAAHLEDKGCGSIDMAGLAQK
                     GGAVYSHVKIGARPEEIHAIRVAAGEADLVLGCDLVVSGSKNVLAAVRKGETGVVVNT
                     AEIFPGDFTRNPDFSLHADRIKSAIRQAAGEGAYFVDATGVAAALLGNSIAANMFMLG
                     YAWQKGLVPLDDASLLRAIALNGEAVEMNQAAFLWGRREAVNSAAVEAIAAPLRPNAS
                     TPALSHSLDEIIERRVSYLTAYQNEAYAKRYLSLLKRAEEAEKAIAPNSHELSEGVAR
                     YYFKLLAIKDEYEVGRLYSDGSFAKQIAAAFEGSPRLEFHLAPPVLGRKNAQGEPVKS
                     SFGPWVLQLFRLLASLKWLRGTVFDVFGYTKERRTERKLISDYEILIDEVLKNLSLEN
                     HAIAVALASIPEKIRGFGHVKTRHVRAAKTEEQALLQQFRAGPAQIKLAAE"
     gene            3211..3298
                     /locus_tag="VNY06_06775"
     tRNA            3211..3298
                     /locus_tag="VNY06_06775"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3245..3247,aa:Leu,seq:caa)
     gene            3475..3768
                     /locus_tag="VNY06_06780"
     CDS             3475..3768
                     /locus_tag="VNY06_06780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX14542.1"
                     /translation="MRSLGSDYRTTQRLGVLETAKAHARLDRALHQLEADPEHEATLS
                     ETEIFDLAVQVLIDQERRAASLPVESRVSEAIENICTLSSFEGNSHHRIASNW"
BASE COUNT          835 a         1042 c         1110 g          838 t
ORIGIN      
        1 ggtacatcga aatatggcgg cgttctcgct cttatgggcg acgatcacac cgccgagtcc
       61 tcgacaaccg cgcatcagtc cgaatttaac tttgtgaatg tgatgattcc tgtcctctcc
      121 cccgcgggcg tgcaggagat cgttgattac ggtctttatg gctatgccat gagccgattc
      181 actagcgcct gggtcggact gaaatgcctg aaggatacgg ttgaatcgac gggatcgatt
      241 gacgtttcgc tggatcgcgt gcggccagtc atccctgctg gcttcatcat gccggagggc
      301 ggtctcaata tacgcaccgg cgacactgtg ctcgaccaag agaagcgcct gcagaactat
      361 aagcgcgacg ctatgctcgc ctggcttcgc gccaaccagc tcaacaaaat cattacatca
      421 ggcggggcgc acgcaaaact cggcgtcatc acggcgggca aatcctatct cgatgtgcga
      481 caagcgatgg atgacctcgg catcgacgag atcgcggcaa ataggcttgg cctgcgtatc
      541 ctgaaactcg cctgcacttg gcctattgag ccgcaaggcc ttcatgaatt tgcgcgcgga
      601 ctagaattga tcatcatcgt tgaggaaaag cggtcgttga tcgaagtaca gctgcgcgag
      661 gagctttatg ggtcgccaag ccagccggtc tgcgtcggca agaaggatga agaggggaac
      721 tggctgttcc cggtgactgg ctcgctcgac gccaatgaca ttgcaatcgc gataggccgc
      781 cgcctgttgc ggttcaatca gagcgaagag ctcgaaaatc gcgtgcgtca actcgaacgc
      841 gcacaggcgg tacttgccgc ggccggagat ttgggcgtcc gtatccccta tttctgctca
      901 ggctgcccgc acaatagttc gacgaggata cccgatggca tgcgtgccta tgcaggcatt
      961 ggctgccatt acatggtcca atggatggac cgttcgaccg aaggctttac ccaaatgggc
     1021 ggggaaggga cgaattggat cggtgaggcg cctttctcga agagaagtca tgtcttccag
     1081 aaccttggcg atggcaccta tgaccattcc ggcatacttg cgattcgctt tgccgtcgct
     1141 tccggcgtca acatcacctt taagattttg ttcaatgatg ccgtcgcgat gaccggcggt
     1201 cagccccttg aaggtggcct gacagtggag atggttgcgc gtcaggtcgc cgccgaaaat
     1261 gtaaagcgca tagttgtcgt cactgacgag ccaaaaaaat atccggcctc cacgcgatgg
     1321 ccggagggtc tcaccgtcaa ggatcgccac gagttcgaca gcgtgcaacg cgagcttgcg
     1381 gctattcccg gcgtgaccgt gctcatttac gatcagactt gcgccgcgga aaaacgtcgc
     1441 cgccgcaaaa agaaattgat gccggatcct gaccggcgag tcctcatcaa cgagcttctt
     1501 tgcgaaggct gcggcgactg cggggcgaaa tccaattgcg tctcgatcca gccgctagaa
     1561 accgaatttg gccgcaagcg catcatcgac caatctagct gcaacaagga cttttcttgt
     1621 ctcgatggat tctgcccggc cttggttacc gtgcacggcg cccgcccgag gaaggcaaaa
     1681 gtcaaagagc agcctgattt tccggagctg ccccgccccg tcattccggc gattggagct
     1741 aaaccctatg gcattctcgt aaccggagtc ggcgggaccg gtgtcgttac gataggcgcg
     1801 atattgggca tggcggcgca tcttgaggac aagggctgcg gatcgatcga tatggcgggc
     1861 ctcgcgcaga aaggcggcgc ggtctatagc cacgtcaaga ttggtgcgcg gcccgaagaa
     1921 attcatgcga taagggtcgc ggccggagag gctgatctgg tgcttggctg cgacctcgtc
     1981 gtctccggct ccaagaacgt gctcgcagcc gtgcgtaagg gcgagacggg cgttgtcgta
     2041 aatactgcgg aaatttttcc aggcgatttt acccgcaatc ccgatttttc gcttcatgcc
     2101 gatcggatca aatcggcgat acgccaggct gcgggagaag gcgcatattt tgtcgatgcc
     2161 accggcgtag cggcggcact ccttggcaat tcaatcgccg ccaatatgtt catgctcggc
     2221 tatgcttggc aaaaaggcct tgtaccgctc gacgatgctt cgctgcttcg cgccatcgcg
     2281 ctcaatgggg aagccgtcga aatgaatcag gcggcgtttt tgtggggccg gcgggaggcg
     2341 gtcaactccg ctgctgtgga agcgattgcc gcgcctttgc ggccgaatgc ctcgacgccg
     2401 gccctttcgc actcgcttga cgaaataatt gagcggcgcg tttcctatct taccgcctat
     2461 cagaatgaag cctatgccaa acgctatttg agtttgttaa agcgcgccga agaagcagag
     2521 aaggcaattg cgcccaatag ccatgaactc tcggagggtg tggcgcgcta ttattttaag
     2581 ctgctcgcga ttaaggacga atatgaggtt gggcggcttt atagcgatgg ttcatttgca
     2641 aagcagatcg cggcggcttt cgagggcagc ccgcgcctcg aattccatct cgccccgccg
     2701 gtgcttggcc gcaaaaatgc gcagggcgag ccagtgaaat caagtttcgg tccatgggtc
     2761 ctacagctat tccgcctcct cgcctcgctc aaatggctgc gtgggacggt cttcgacgtt
     2821 ttcggctaca ccaaagagcg ccgtaccgag cgaaaactta ttagcgatta tgaaattctc
     2881 atagacgaag ttttgaagaa tctctccctt gagaatcatg cgatcgctgt tgcgcttgct
     2941 tctatccccg agaagatacg cggcttcggc catgtcaaaa caaggcatgt tcgggccgcg
     3001 aaaaccgagg agcaagcttt gttgcagcaa tttcgtgcgg gtcctgcgca gataaaactc
     3061 gcagcggaat aggcttttgg tttaggatgg tgcaacaagc tcaagtccca gtccatgggc
     3121 tggacttgcg cttcaatctt gtggcagatt gagccaattc tgcgccgtgg atggtctatc
     3181 ccgtgcgcgc ctattgtatg tgcgctttat gcgggcgtgg cggaactggt agacgcgctg
     3241 gactcaaaat ccagttctgg ttgacagagt gtcggttcga ctccgaccgc ccgcaccatc
     3301 accattgatt tcactggaaa agcctaaatt tacatgactt ttggacagaa aagtatacgt
     3361 tcggcgcata cagaagcgcg cccagaaacg atgcccaaag tccgatatct gcaacgtcac
     3421 gggaacacgt ggctatttgg attgtgcgtc ccgcgtgaag gcaagcgaga aatcttgcga
     3481 tcactcggtt ccgattaccg gacgacgcaa cggcttggtg tccttgaaac agcgaaggcg
     3541 catgcgcgac tcgaccgtgc cctccatcaa cttgaagcag acccggaaca tgaggccacg
     3601 ctatccgaaa ccgagatttt tgatttggcg gtgcaagttt tgatcgacca ggaacgccgt
     3661 gccgcatctt tgccggtcga gagccgggtt tcagaggcaa tcgagaacat ttgcacgcta
     3721 agttcattcg agggaaactc acatcatcgt attgcgagca actggtaggg cgcgaaacct
     3781 tgcgagttcg caaggttgac cgggtcagcc atgaacggct cttag
//
LOCUS       DATMPU010000297         3178 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_1171800,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000297 DATMPU010000000
VERSION     DATMPU010000297.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 3178)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3178)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3178
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1171800"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            166..1341
                     /locus_tag="VNY06_06785"
     CDS             166..1341
                     /locus_tag="VNY06_06785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HWX14543.1"
                     /translation="MGRDTRARIWSASNTELIALIDLIYEAALDNDLWPGVLINLADA
                     MGAAQVAMPSMDLRANVVATIAPRCGPDLLTSWKEYWAFRDPVLARAMRRPAGEIYTL
                     DSLMPREEFAATPVFNELWLPAQYSLATMGANLVVEDQFSALICFSNPPGKDSLTAEQ
                     MRIFEGVVPHLARALRINRRLWEMELKHVAATERFEAFSQGALLADASGRVVHANAAA
                     KAMLDGGSGIILDKGRLAAIGCPDILQKLIASCAQTTLALGGPGGEFKIPREIPRSPL
                     DVTVTPLRSKTRLADVPWIGVGVPVAIVTVSDPDIDRRLLVVNLRRRFGLTAAESRLA
                     AEILKGDGRAATARRRGISTATAKTHLSSIFEKTGTHRQAELIRLLLDAADGDEIEI"
     gene            1644..2558
                     /locus_tag="VNY06_06790"
     CDS             1644..2558
                     /locus_tag="VNY06_06790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX14544.1"
                     /translation="MEDDLLALINLIYEASFDSTLWPTALISLADTTGTAQVSVAAMD
                     RRAQTYDWISPRTDPVMRESYKNYWAFHNPFRLLTEQVPAGEVFSLERIMSRKEFSAT
                     PVFNEWFRPAEFGLAILAVNLGVRSQVSTLLCVTNAPKNDHITGKQTLAFKAALPHID
                     RAIRIHRELRMADLDHDTAPGRLESLQRSVMLVDGAATVLFANAAARTLLNSGGGLTL
                     KAGCLHSTDSADAVQGLIATCMPKAHALSGFGGEILIHRGPRRPPLRVTVTPLRSKGT
                     VAELPWLGLSIPVAIVTVADPVSEKRMN"
     gene            2580..2979
                     /locus_tag="VNY06_06795"
                     /pseudo
     CDS             2580..2979
                     /locus_tag="VNY06_06795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086557984.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          663 a          915 c          910 g          690 t
ORIGIN      
        1 cgaatgccgg atgaagaaaa ttcatcgagg aaaacagctg atccgagaac ggagaacgag
       61 tagatttgag cgggctttac aatccgcgga gggataagta cgtttgttgc ggcctagcct
      121 ctggtcgata ccaaatcgct ttgcctcaaa attgaggaag ccgccatggg tcgcgatacg
      181 cgagcgcgaa tatggagcgc ttcgaataca gagctcattg ctctgatcga cttgatctat
      241 gaggcagcgc tcgacaatga tctctggccg ggagtgctga taaatcttgc cgatgccatg
      301 ggcgcggcgc aggtcgcaat gccctcgatg gatctgcgtg ccaatgtggt cgcgaccatt
      361 gcgccgcgct gtggtcctga tttgctgacg tcttggaagg aatattgggc gtttcgcgat
      421 ccagttctgg cgcgagctat gcgtcgcccg gcgggcgaaa tctatactct cgacagtctg
      481 atgccgcgag aggagttcgc tgccaccccg gtttttaatg agttatggct gccggcccaa
      541 tatagtctcg cgacaatggg tgccaatttg gtggtcgaag atcagttttc agccttgatt
      601 tgcttttcca acccgcccgg caaggattcg ctgaccgccg agcaaatgcg tattttcgaa
      661 ggggtggtgc cgcacctcgc ccgggccttg cgcataaatc gccggctctg ggagatggag
      721 ctcaagcacg tcgccgcgac ggagcggttc gaagcctttt cacagggcgc tttgctggcc
      781 gatgcctcgg gcagagtggt tcacgcgaac gccgccgcga aagcgatgct cgacgggggc
      841 agcggaataa ttctggacaa gggacgtctc gccgccattg gctgcccgga tattttgcaa
      901 aagctgatcg cctcctgcgc acaaacgact ctcgcgcttg gtggccccgg cggagaattt
      961 aaaatcccgc gcgagattcc ccgttcgccc ctcgatgtga cggtaacgcc gcttcgctcc
     1021 aagacccggc tcgcggacgt tccctggatc ggcgtcgggg ttcccgtcgc gatcgtcaca
     1081 gtaagtgacc ccgatatcga tcggcggctg ctagtggtta atctgcgccg ccgctttggc
     1141 ctgaccgccg cggagtcgag gctggccgcc gagattttga aaggggacgg ccgcgcggcc
     1201 accgcgcgac ggcgcggcat ctctaccgcg acggccaaaa cccacctttc gagcattttc
     1261 gagaagaccg gaacccaccg gcaagctgaa ctcatccgtc tcctgctcga cgccgccgat
     1321 ggggacgaga tcgaaatatg agcggttccg gcgttgccgc cgccaaggcc aatcgcccca
     1381 tttgcaagga tcgcggcagg cttcaaacaa ttgttaccta tcgaagcagt caaccgcgag
     1441 tggcatgcca cgttttgttt tgcacggggc tttatttcgc caaaatgaca tcgaaaacga
     1501 tgtgctaatg ttctagtgtt ctccttggga aactggtgca gcgagctttt agctcaggcc
     1561 cctttccttg gaaatgaacc gggcctcaaa gggctgaccg gcacctcggg aatgataaaa
     1621 taggctcagg aatatttttc gtcatggaag acgaccttct tgccttgata aacctaatct
     1681 acgaggccag tttcgacagc actctatggc cgacggccct aataagcctt gccgacacca
     1741 cagggacggc gcaggttagc gtggccgcca tggaccgtcg cgcccaaact tacgattgga
     1801 tctccccgcg taccgatccg gtcatgagag agagctacaa gaactattgg gctttccata
     1861 acccgttccg gcttctcact gagcaagtgc cagccggcga ggtattttcc ctcgaaagaa
     1921 ttatgtcgcg aaaggaattc tccgctaccc ccgtgttcaa cgagtggttt cgcccggccg
     1981 aattcggcct tgcgatactg gctgtcaatt tgggcgtcag gagccaggtt tcgacattgc
     2041 tctgcgtcac taatgcgcca aagaacgatc acataacagg caagcagacg cttgccttca
     2101 aggccgcgct gccgcacatc gaccgcgcca ttcgtattca tcgtgagctt cggatggccg
     2161 atctcgacca tgataccgcc cccggacggc ttgagtcctt gcaacgaagc gtgatgctgg
     2221 tcgatggcgc cgcgacggtg ctgttcgcca atgcggcggc gcggacactg ctcaattctg
     2281 gcggcgggct caccctcaaa gccggctgcc ttcacagcac cgacagtgcg gacgcagtac
     2341 aaggattaat cgccacctgc atgcccaagg cgcacgcgct aagcggtttc ggcggagaaa
     2401 ttttgattca tcgcggcccg cgccgtccac ccctgcgtgt gacggtgacg ccgttgcggt
     2461 caaagggcac cgttgcggaa cttccctggc tcggcctgag cattcccgtg gccattgtca
     2521 ccgtcgccga tccggttagt gaaaaaagga tgaactgagt ggccgtcaca tcaatcgccg
     2581 gtcagatccc gcaccgaggc tttcatccgc tcatcgagca cagacttgcg tcgttgaaat
     2641 atcagcgtac cgcagcgcgg atcaaagagg gcaaacgcgc gatcaagtgg acgcggttgt
     2701 cgtttcgttc ctttgccctc aatgcgattc gtctccagct tcatgcattg gcctacactt
     2761 tcggtaattt cctgcgcacg ctggcgcgcg gagccgatca aagactgttc gctcacgagt
     2821 ctccgcgaga gactcatcaa gattggtgcg aaagtcgtga gcaatggccg ttacgtcacg
     2881 ttccagatgg ccgagatctc cattccgaga aatttgttcg ccgacattct gcggctgatt
     2941 gcggaactac ggccgccacc tgatccgtta cctaggtgaa gtgttctgtt gtcatgaaga
     3001 cgattgtgtg tggctggttt gggtccaaaa gcgccacaaa aattatccgc gtctcaaccg
     3061 ttcggctgat gcgtcgactg cttaagccaa ttatcttcct cctcaagcga aatgaaattt
     3121 cgggaatcaa attgggcaat ccgagccaga aaggccaaat cgagccagtt tgggggtg
//
LOCUS       DATMPU010000298         4971 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_3947904,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000298 DATMPU010000000
VERSION     DATMPU010000298.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 4971)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4971)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4971
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3947904"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..644)
                     /locus_tag="VNY06_06800"
     CDS             complement(<1..644)
                     /locus_tag="VNY06_06800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012384273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HWX14545.1"
                     /translation="MKYRELGTSGPRVSAISLGSWLTFSGGIEKRQALACIHKALDLG
                     ITFFDTANVYGRGAAESVLGEALAGMGRDSYILATKLFFAMSPTDQGLSRAQIAKQLD
                     ASLRRLGTDYVDLYQCHRYDPETPLHDTMEALTAAVGQGKVRYIGFSEWPLDKIELAA
                     AMPGVERFVSSQPQYSMLWPAPQDEIFPRCAELGIGQIVWSPLAQGVLTGKYRPG"
     gene            complement(983..1303)
                     /locus_tag="VNY06_06805"
     CDS             complement(983..1303)
                     /locus_tag="VNY06_06805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012590849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SWIB/MDM2 domain-containing protein"
                     /protein_id="HWX14546.1"
                     /translation="MTTTGKKATPATGKTSKPPAAKKPASGANAFAKPLQPSKELAAV
                     VGSDPLPRTEVVSKMWDYIKKHKLQNEANKREILADEKLAAVFGKSKVTMFEMNKFLA
                     QHLK"
     gene            complement(1680..2552)
                     /locus_tag="VNY06_06810"
     CDS             complement(1680..2552)
                     /locus_tag="VNY06_06810"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017484286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-32"
                     /protein_id="HWX14547.1"
                     /translation="MAQLAEVAQSLLTAALAAPYLDRDEEYELASRWKLQADEKAMHL
                     LARAHMRLVIALAARFRHYGLSLADLIQEGHVGLLEAAARFEPDRDVRFSTYATWWIR
                     AAMQNYILHNWSIVRGGTSSAQKALFFNLRRIRAKLAQGGSAADSVIFAQIAEVLGVS
                     FSDVESMGARLCGPNLSLNAPVGESDPFSPRELIGMLVDDRPRPDELAGLAIDSGRRT
                     AWLNEALTALSERERKILCERRLTEETATLEALGDRLGISKERVRQIESRAMEKLRIE
                     LTKRHPEDAAEVMV"
     gene            3577..4140
                     /locus_tag="VNY06_06815"
     CDS             3577..4140
                     /locus_tag="VNY06_06815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012384051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HWX14548.1"
                     /translation="MFIETEATPNPATLKFLPGRPVMTHGTRDFRDAAGAGQSPLAEA
                     LFAVDGVSGVFFGRDFISVTKSSGDWADLKPIILGAIMEHFLTGEPLLRAACDGTRQE
                     DSDEFFDDDDAETVGTIKNLIESHVRPAVANDGGDIRFRGFRDGIVYLAMKGACSGCP
                     SSTATLRHGIENLLKHYVSNVRSVEQI"
     gene            4210..4899
                     /gene="tsaB"
                     /locus_tag="VNY06_06820"
     CDS             4210..4899
                     /gene="tsaB"
                     /locus_tag="VNY06_06820"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012384052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HWX14549.1"
                     /translation="MKILAIDTALPAVSACVLDDAAEGPEAIETMPMERGHAEALLPL
                     IERVMAFVEGGFASLDRVAVTVGPGSFTGLRVGIAAARAIGLACKVPVTGVSTLAALA
                     APLILEQKPGLVVAAIDARRGNVFFAAFGPIGRAVVSPRMAQAREAARSLGAGPVRLA
                     GSGARLLAAEAAAHGTVAEIARDAAGVDIAFVAKLGLLADPSLTPPRPLYLASPAGKV
                     PEASQRQPIAP"
BASE COUNT          992 a         1500 c         1393 g         1086 t
ORIGIN      
        1 ccggggcggt acttgcccgt gagcacgccc tgcgcgagcg gcgaccagac gatctggcca
       61 atgccaagtt ccgcgcagcg aggaaagatt tcgtcttgcg gagcgggcca cagcattgag
      121 tactgcggct gactcgatac gaaccgttca acgccgggca ttgctgccgc caactcgatt
      181 ttatcaagcg gccattcgct aaatccgata tagcgcactt tgccttgacc gaccgccgcg
      241 gtcaaagctt ccatcgtgtc gtggagtggg gtctcgggat catagcgatg gcattgataa
      301 agatcgacat aatctgtgcc gagccgccgc agcgacgcat cgagctgctt tgcgatctgg
      361 gcgcgagaca gcccttgatc agtggggctc atcgcgaaaa agagttttgt cgccagaata
      421 taggaatcgc gccccattcc ggcaagcgct tcaccgagca cgctttccgc agcaccgcgg
      481 ccatagacgt ttgcggtatc aaagaacgtt attccgaggt caagcgcctt gtgtatgcag
      541 gcgagcgcct gccgcttttc gatgccgccc gaaaaagtca gccaggaacc aagcgaaatc
      601 gccgaaacac gcgggccgct tgtgccaagc tcgcgatatt tcacagcgaa actcccaaac
      661 caaactcctg aagggggcga gattgcgagc tttggcaggt cattggctgg gatgaagagt
      721 tctggccgta aagctccgtc gtggaactcg cctgcgcaag gcgcgagtta gagtcgaaat
      781 ataagcttgt ctcatcggtc agtcctcaac atccgcgaag aatgcccggc gcgagcgcgg
      841 atcaacaaaa tatccgaaag ctagccgtgg atatttcgaa aatcatgtag aaatcggttt
      901 gctcactgca agtgattgat accgcgccat ccgtttcgtg acgaccccga tatggcaaaa
      961 ggggcaccga ccgtgcggct gcctacttta aatgctgggc gaggaatttg ttcatctcga
     1021 acattgtcac tttgcttttg ccgaacaccg cggccagttt ctcatccgcg agaatttccc
     1081 gcttattggc ttcgttttgc agcttgtgct tcttgatgta gtcccacatc ttactcacga
     1141 cctctgtgcg gggcaaaggg tctgatccga ccaccgcggc aagctctttc gaaggctgca
     1201 atggcttggc aaaagcattt gccccggatg cgggcttttt cgccgcaggc ggttttgaag
     1261 tcttacccgt cgcgggcgtg gcctttttac ctgtcgtcgt catccgcttt acctccatag
     1321 atttgcggca gggcctgccg gggcaaaatg cctcccgccc gcattgttga tatgagcttg
     1381 gctcgacgga accccaaccg atgcgctccg tcaagcggtc tgcacaatct ttattgatgc
     1441 ttacgcgcct taagctccgg tttcgccgcc acaaagcaac tcgtcggtct aaaagggaag
     1501 cggcgccgga gcaggtacag aaccccacgc ccgttgccga ttcagctttt taagccactt
     1561 cgtcccagtt tggctgatat tcggtagaaa tcgccgaaag cggccgaaag ttttggggaa
     1621 aactggcctc acagaagaat tggctcgggc attctggcct tcccgggtcc cgcccatgct
     1681 taaaccatca cctccgccgc gtcttcagga tggcgttttg tcagctcgat tcgcaatttt
     1741 tccatggccc ggctctcgat ttggcgtacc ctctccttgg aaatgcccag gcgatcgcca
     1801 agcgcttcga gcgttgccgt ttcctcggtt agccgccgct cgcacaatat cttgcgctcg
     1861 cgctcggaaa gagccgtcaa agcctcattt agccaagcag tccgccggcc actgtcgata
     1921 gcaaggccgg ccaactcgtc cgggcgtggc ctgtcatcga caagcattcc gatcagctcc
     1981 cgaggggaaa acggatcgct ttctccaacc ggcgcattca aggacagatt gggaccacac
     2041 aaacgggcgc ccatgctttc gacgtctgaa aaggacactc ccagcacctc ggcaatttga
     2101 gcgaagatta cagaatcggc agctgaaccg ccttgtgcca atttcgcgcg aatccggcga
     2161 agattgaaga aaagagcttt ttgggccgaa ctcgtcccgc cccgcacaat agaccaattg
     2221 tgcaaaatgt aattctgcat tgcagcgcgg atccaccagg tcgcataagt cgaaaaacgc
     2281 acatcgcgat ccggctcgaa gcgcgctgcc gcttccaaaa gcccaacatg gccttcctga
     2341 atgaggtcag caagcgacaa tccgtagtgg cgaaagcgcg cagcaagagc aattacgagg
     2401 cgcatatggg cgcgcgcgag aagatgcatg gccttttcat cggcctgaag cttccagcgg
     2461 gaggccagct catactcctc atcgcggtca agataagggg cggcaagcgc cgcagtgaga
     2521 agactttgcg cgacttccgc taattgagcc atggttcttg cccccgcaca gcggcaaatc
     2581 ccttgccaga gctcgctcgc tatcccgagg ccgcccaaag gcgcaacttt ttcgccgcgt
     2641 cagtaaccgc ctgccgctta caaatgccag tcagggaagc gagtctccgc ccatctatca
     2701 acgattgccc ggcgccgctg gttccaactg gtcacctttt gatcatcccc gccgtccgcc
     2761 agctccttaa ggcgagtaca gccatggctg cccgaacacg ttcgggcaaa aatcccctcg
     2821 ctaatggatt tgaatgccga attcatttcc cccatgtgat ttctcgccgc agctgattat
     2881 cactgctgtg gcgcaaaatt tccgcctatt gtgcgttgcc acgaaatcaa tcgatcgtta
     2941 ggtggattcg gcctcggaac cctcctcttc cttcatcgaa aagctcactc agtggaaatc
     3001 aagaaagccc ggccggcgtt ggcggaagac gaatcgaaaa gtgattcgac ggcgccaacg
     3061 acgccggttt ggcaaccgat ccaggcattc ttaatctacc gcatttccgg gggccttctt
     3121 tttcgacacg gccaccagca tcgcttcggc actggataag cttgcggcgc cgctgctcga
     3181 ttcgacagtt gcggtgacgc tgaacggatt tgccaggaag gccgcgcgcg ccggcgccgt
     3241 ggtctatgtc actggggtaa ccaagccggt gcgccgcgcc cggatcaccc atgggatccg
     3301 cccgccccgc gtccggttcc ggacggatat cactgctgcg ataagcattt gcccgaagac
     3361 acctaaacac cccgtccgcg cgggattgaa gcgtctgtgc cctgaagagc gttgcggttc
     3421 ggtaaggctc gattccgcac ccaagcggga tttgcggcgg agcgattctc gctcctacgc
     3481 gcctaagtgt gacttgccca ttcgcccgct tcacatcata ttttagaaac gttccggttg
     3541 cggcccccgg cttgttccgg ctgcttggag agacccatgt ttatcgagac cgaagccacg
     3601 cctaatcccg cgaccttgaa atttctccct gggcgcccgg ttatgaccca tggcacgcgc
     3661 gacttccgcg atgctgccgg cgcgggtcaa tcgccacttg ctgaggcact gtttgcagtc
     3721 gatggcgtat cgggcgtttt ttttggccgg gactttattt ctgtcacgaa atcgagcggg
     3781 gattgggcgg acctcaaacc catcattctt ggcgctatta tggaacattt cctcaccggc
     3841 gagcctttgc tccgcgccgc gtgcgatggg accaggcaag aagacagcga cgaatttttt
     3901 gacgatgacg acgccgaaac cgtcggcacg atcaagaact tgatcgagtc ccacgtgcgg
     3961 ccggcggtcg ccaacgacgg cggcgatatt cgattccgtg gatttcgcga cggaatcgtc
     4021 tatctcgcca tgaaaggggc gtgttccggt tgtccctctt caaccgctac gctccggcat
     4081 gggattgaga atttgctcaa gcactacgtt tcaaatgtgc gctctgtcga acagatttga
     4141 tttccggttt tttcatcgga tttttcttgg ctctccgacg aggacgttac gagaaccgag
     4201 ttctccggca tgaagattct ggctatcgac actgcacttc ccgccgtttc ggcctgcgtc
     4261 cttgacgacg cggccgaggg tccggaagcg atcgaaacga tgccgatgga gcgtggtcac
     4321 gccgaggcgc tcttgccgct gatagaacgg gttatggctt tcgtcgaagg tggttttgcg
     4381 tcgctcgatc gggtcgctgt gacggtcggg ccaggttcct tcactggact gcgtgtggga
     4441 attgccgccg cgcgcgcgat tgggctcgcg tgcaaagttc cggttacagg ggtttcaaca
     4501 ctagctgccc ttgcggcccc gttgatcctt gagcagaagc caggtctcgt ggtcgccgca
     4561 atcgatgccc gccgaggcaa tgttttcttc gccgccttcg gcccgatagg cagggctgtc
     4621 gtttcgcctc gaatggcgca agcccgcgag gccgctcgct cgcttggcgc gggtccagtg
     4681 cggcttgccg gatcgggcgc acgtctcctc gcggcagagg ctgcggccca tgggaccgtc
     4741 gcggaaattg cccgtgatgc cgccggcgtg gacatcgctt ttgttgcgaa acttggtctt
     4801 ctcgccgacc caagcctcac tccgccgcga cctctttatc tggcgagccc tgctggcaag
     4861 gtgccggaag catcgcaacg tcaaccaatt gcgccgtgag ctgctcatga tcgcttggcc
     4921 cgccaaagct gccgctcgta ttcgaccgat tggagtcgag cgaagtgcgg a
//
LOCUS       DATMPU010000299         9871 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_4554183,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000299 DATMPU010000000
VERSION     DATMPU010000299.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 9871)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9871)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9871
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4554183"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(382..1494)
                     /gene="leuB"
                     /locus_tag="VNY06_06825"
     CDS             complement(382..1494)
                     /gene="leuB"
                     /locus_tag="VNY06_06825"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012592468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HWX14550.1"
                     /translation="MTNFKLFVLPGDGIGPEVMAEAEKIAAWLTEAGIAHFEIEKGLV
                     GGAAYDAHGTAISEADIARAQAADAVLLAAVGGPKWNDAPYDVRPEAGLLRLRKDLKL
                     FANLRPAICYPALADASSLKRELVDGLDLMIVRELTGGVYFGEPKQIIDLGNGQKRAI
                     DTQVYDTFEIERIGRIAFELARKRRNKVTSSEKHNVMKSGVLWRDVITSLHAREYPDV
                     ELEHQLADALGMQLVRRPKQFDVIVTDNLFGDMLSDVAAMLTGSLGMLPSASLGEKGG
                     TGETRKALYEPVHGSAPDIAGKGIANPIAMIGSFSMALLYSFDLGDLSDRIETAISSV
                     LAKGLRTADIAGGATATLSTAEMGDAILASLQASLR"
     gene            complement(1574..4303)
                     /gene="acnA"
                     /locus_tag="VNY06_06830"
     CDS             complement(1574..4303)
                     /gene="acnA"
                     /locus_tag="VNY06_06830"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007598848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HWX14551.1"
                     /translation="MTSLDSFKCRKKLNVGAKTYHYFSLKAAEKNGLAGISELPFSMK
                     ILLENLLRFEDGRTVTKADIEAVAAWAGNKGTAEREIAFRPARVLMQDFTGVPAVVDL
                     AAMRDAMTKLGGDPQKINPLVPVDLAIDHSVIVDAFGSSKAFKTNVELEYSRNGERYR
                     FLKWGQHAFDNFRVVPPGTGICHQVNLEYLAQTVWTAKEKSAPVRGKHATVEIAYPDT
                     LVGTDSHTTMVNGLSVLGWGVGGIEAEACMLGQPLSMLLPEVIGFKLTGALGTGITAT
                     DLVLTVTQMLRQKGVVGKFVEFYGPGLNHLSLADRATIANMAPEYGATCGFFPIDSET
                     LSYLETSARAPARVALVEAYAKAQGLLRTRTTPDPIFTDKLELELESVEPSMAGPKRP
                     EGRVALSAVGTGFRTALESEYGKAGETLERFPVEEKNFDLGHGDVVIAAITSCTNTSN
                     PNVLIAAGLLARNAVEKGIGVKPWVKTSLAPGSQVVAEYLAASGLQKSLDKLGFNLIG
                     FGCTTCIGNSGPLAPEISKAINDNGIVAAAVLSGNRNFEGRVSPDVQANYLASPPLVV
                     AHALAGTVATDLARDPLGHDKKGNPVFLKDIWPDQEEIAAFIGKFVTKKVFKARYADV
                     FKGDAHWRKVKAPAAETYKWDIGSTYVRNPPYFAGLKMQPEPVHDIVNARILALFGDK
                     ITTDHISPAGSIKEASPAGQYLLARQISKADFNQYGTRRGNHEIMMRGTFANIRIKNH
                     IRQLPDGLVPEGGLTKHWPDGEEMPIFDAAMKYESEGVPLVVFAGVEYGNGSSRDWAA
                     KGTKLLGVRAVIAESFERIHRSNLVGMGVLPLTFEPGTSWKSLGLKGDEEVTIKGLGS
                     SLKPRQAMEAEITFADGRVEKVPLICRIATLDELEYFNNGGILPFVLRQLAAV"
     gene            4391..5035
                     /gene="ccmA"
                     /locus_tag="VNY06_06835"
     CDS             4391..5035
                     /gene="ccmA"
                     /locus_tag="VNY06_06835"
                     /EC_number="7.6.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007561669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015439 - ABC-type heme transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC exporter ATP-binding protein CcmA"
                     /protein_id="HWX14552.1"
                     /translation="MKPAAMLARGSITMQLCASNLTIARGGRTIIASLSFALCAGECL
                     ALTGPNGAGKSTLLRALAGLLPLAAGTISLSPLSEEFVPQQAHYVGHADGLKGLLTVT
                     ENLEFFAAILDVGMGGLPVKTALGKLGLAHAAKLPVAYLSAGQKRRVALARLLAVRRP
                     IWLLDEPLTALDTASRVLLGQIMTEHLTQGGLIVAATHAGLDLKGQELRLRAAG"
     gene            5032..5730
                     /gene="ccmB"
                     /locus_tag="VNY06_06840"
     CDS             5032..5730
                     /gene="ccmB"
                     /locus_tag="VNY06_06840"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020174413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HWX14553.1"
                     /translation="MTTPKGTPKETSPLAALFLRDIRTGRRIGGGTSLGIVFFLILVT
                     LCAFAIGPDLRLLGKIAPAILWISGLLSTLLGLDRLFQADHEDGSLDLLLTSSTPLEL
                     IILVKCLAHWILTGLPLAATAPIFGLMLNLPLSELWSVTLSLLAGTPSLTLTGAIGAA
                     LTVSLRRGGLLFAVLILPLCVPSLIFGVAAAGAGEASLVPFATPFLILCALTLIAGVV
                     APVAAAAALRNGGR"
     gene            6256..7044
                     /locus_tag="VNY06_06845"
     CDS             6256..7044
                     /locus_tag="VNY06_06845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011661561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWX14554.1"
                     /translation="MGLPPRAERFAPPALVFAALLSAWESYVRFGDVPPYVLPSPTLI
                     FSTLISDWATLFPALLVTLRTTLGALAAAVIGGVGLALLFTQWRWVERSFLPFAIVLQ
                     VTPVVAIAPLLLIYLDAPAAVLVCAFLVAFFPILSSTALGVASADRNLVDLFKLYRAT
                     RWQTLLQLRLPSALPYFLGGLRIGGGLALIGAIVAELAAGNAGKGAGLAFRIAEAGYR
                     LNIPRMFAALTLIGLTGIAIYAAFAALSSYLLGRWHESAAGPGN"
     gene            7460..9130
                     /locus_tag="VNY06_06850"
     CDS             7460..9130
                     /locus_tag="VNY06_06850"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HWX14555.1"
                     /translation="MGLNNMFVDTRQLEEGSVIRSEVCIIGGGVAGISLALEFEKHGI
                     ETCLLESGGFKADEATRDLYRGESVGIPYRFADGCRGRFLGGSSNCWGGWCQPLQEHD
                     FERREWVPNSGWPFGKSELEPYYERSHSLLKLGPNRYDVDFWVDAIGKPQIQPIALNA
                     ARVVTGISQFSPPARFGTLYRDELAKSKSTRVYLYANAVDIEVDADRAIRSVQVKTLT
                     GRAVVAAAKIFVLATGGIENARLLLASNKVRANGLGNDNDLVGRFFMDHPRVFTGKIH
                     FRKGWSGHMLYDVLFNYLNKAIAAHGACVAAQFALSPQLQANERLLNARICFSSLFFG
                     QDTEAREALGRIKHRFARDAEFDQSLAHDIFAVSAHPTLLAQLVLARVLRRPQLIKHS
                     RFQAIVEPLPDPASRVTLSSECDRLGVNRVKVDWRLGAQVKHTFDRTLAIIAEELTSA
                     GVADVVLDPPIEGGDWPSSFEKEGSWHHMGTTRMDDSPKLGVVDRKCRVHGTSNLYVA
                     GSSVFPTAGGDFPTITIVALALRLSDHIAGEMRQPAVLRGPMKEALQA"
     regulatory      9223..9321
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            9386..>9871
                     /locus_tag="VNY06_06855"
     CDS             9386..>9871
                     /locus_tag="VNY06_06855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020174115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HWX14556.1"
                     /translation="MNIYEKPQELVPQSVTCGPLPGSRRVYSQVQGHSDIAVPFREIV
                     LDPLADEPPLRVYDTSGPYTEDGVSIDLSKGLAPIREPWLARREGLEIYCGRAVRPED
                     NGGVAVGRLVPPCPANRLPHKGCQDAPVTQYEFARAGIITEEMIFAAARENLGREKIA
                     DD"
BASE COUNT         1944 a         2888 c         2873 g         2166 t
ORIGIN      
        1 cgagaccgag ccacggtgcg gcgatcacca aagcggtgga tttggacctg gcatacgaat
       61 ggtacactcg ctaaacgata gcttttcaac agatttggaa gccgccgcgc aactctgcgc
      121 agccgggggt tgtcgaagca gcatggttcc aactgagacg acggaactcg ccgaatgttt
      181 gattaccgat tgtctgactt catacgcttt ctcgaaagag cggcacgcgc ggtgaacggc
      241 gggcttcgtc gatacgagaa ggttatcaaa atgcttagac aatgcgagcg cggcccttgc
      301 gcgcagaaca ccgcgcttcc agaagaaggc agagcattgc ctcagcgagt aaccgggcaa
      361 gaggtagccg ctgaacagat cctatctgag acttgcctgt aaactggcga gtatggcgtc
      421 ccccatctcg gcagtcgaaa gggtcgctgt tgccccgccg gcaatgtcgg cggtgcgcag
      481 ccctttggcg agcacgctcg agatcgccgt ctcaatgcga tcggagaggt cgccaagatc
      541 gaacgaatag agcagggcca tcgagaagga gccgatcatc gcaatcggat tagcgatccc
      601 cttgccagcg atatcgggag cggagccatg caccggctca taaagcgcct tgcgggtttc
      661 ccctgtcccg cctttttcgc caagcgatgc ggaaggcagc attccgagcg agccggtcag
      721 catggcggcg acgtccgaaa gcatgtcgcc aaagagattg tcggtcacaa tgacgtcaaa
      781 ctgctttggc cggcgcacga gctgcatgcc aagcgcatcg gcaagctggt gttcgagctc
      841 gacatccgga tattcccgcg cgtgcaagga tgtgatcacg tcacgccaga gcacgcccga
      901 tttcatcaca ttatgttttt cggaagacgt aaccttgttg cggcgcttgc gtgcgagctc
      961 gaaagcgatc cgcccgatcc gttcgatctc aaaagtgtca tagacctgcg tatcgatcgc
     1021 ccgcttctgc ccattgccaa gatcgatgat ttgtttgggt tcgccgaagt agacaccgcc
     1081 agtgagttcg cggacgatca tcaaatcaag accgtcgaca agttcgcgct tgagcgaaga
     1141 cgcatcggcc agcgcgggat agcaaatcgc cgggcgcaga ttggcaaaga gctttagatc
     1201 cttgcgcagc cgcaagagtc cagcctcggg ccggacgtcg tagggagcgt cattccattt
     1261 cggcccgccg accgcggcaa gcaacaccgc gtccgcagct tgcgcgcgcg ctatgtcagc
     1321 ttcactgatc gccgtgccat gcgcgtcata ggccgccccg ccgacgaggc ccttttcgat
     1381 ctcgaaatga gcaatgcccg cctcagtcag ccaagctgca attttctcgg cctcggccat
     1441 gacttccggg ccgatcccgt cccccggaag aacgaaaagc ttaaagttag tcatgtgtct
     1501 tgtcctcaag gacggcccgg gcggcggaag ccgaagggct ctcgccacca caatcttcaa
     1561 gcgggaaaga actttatacc gctgcaagct ggcgcagcac gaagggaaga ataccgccgt
     1621 tgttgaaata ttcgagctca tcgagcgtcg cgatgcggca gataagcggc actttctcca
     1681 cccgcccgtc agcgaatgta atttctgctt ccatcgcctg gcgcggtttc aaactgctgc
     1741 cgagcccctt gatggtgacc tcttcgtccc ccttgagccc gagcgatttc cagctcgtgc
     1801 cgggctcgaa ggtaagcggc aagacgccca tgccgacaag gtttgagcgg tggatgcgct
     1861 caaaggattc ggcgatgacc gcgcgaacgc ccaagagctt tgtccccttg gcggcccaat
     1921 cgcgcgaaga accattacca tattcgacac cggcgaaaac gacgagcggc acgccttcgc
     1981 tctcatattt catcgccgcg tcgaaaatcg gcatctcctc gccatccggc caatgtttcg
     2041 tgaggccgcc ttccggcacg aggccatctg gcaactgccg gatgtggttt ttgattctta
     2101 tgttggcgaa ggtgccgcgc atcatgattt catgattgcc ccggcgcgtt ccatattggt
     2161 tgaaatcggc cttcgaaatt tgccgcgcga gcagatattg gcccgccggg gaggcctcct
     2221 tgatcgagcc cgcgggggaa atatggtcgg tcgttatctt atcgccaaaa agcgctagaa
     2281 tgcgtgcatt gacgatgtca tgcacgggct ctggctgcat ctttaatcct gcgaaataag
     2341 gcggattgcg tacataggtc gagccgatat cccatttata ggtctcggcc gccggcgcct
     2401 tcaccttgcg ccaatgcgcg tcgcccttaa atacatccgc gtagcgggct ttaaaaactt
     2461 ttttggtcac gaatttgccg atgaacgcgg caatctcttc ctgatctggc cagatgtcct
     2521 tgagaaaaac cgggttgccc tttttgtcgt ggccgagcgg atcgcgtgct aaatccgttg
     2581 ccaccgtacc cgcgagcgca tgggcaacaa ccaagggcgg cgaagcaaga tagtttgcct
     2641 gaacgtcggg gcttacccgg ccctcgaaat tgcggttgcc ggaaaggacc gcggccgcaa
     2701 cgatcccatt gtcattgatg gctttcgaaa tctccggcgc caagggaccg gaattgccga
     2761 tgcaggtcgt gcagccgaag ccaataagat tgaagccaag cttgtcgagc gatttttgta
     2821 gacccgatgc ggcaagatat tcggcgacca cttggctccc aggagccagc gaggtcttga
     2881 cccaaggctt tacaccgatg cctttttcga ctgcgttgcg cgcaagaagc cccgcagcaa
     2941 tgagcacatt cggattcgag gtgttcgtgc aggaggtgat cgccgcgatc accacatcgc
     3001 catgcccgag atcgaaattt ttttcctcga ccgggaaccg ctctagcgtc tcgccggcct
     3061 tgccatattc actctcaagc gcggtccgaa aacctgtccc gactgcactc agcgcgacgc
     3121 gcccttcggg gcgtttcggc ccagccatcg acggctcaac cgattcgagt tcgagctcga
     3181 gcttatccgt aaagattgga tcgggggtcg tccgcgtccg gagcaagcct tgcgctttcg
     3241 cataggcctc gacgagcgcc acccgcgccg gcgcccgcgc cgacgtttca agatagctga
     3301 gcgtctctga atcgatcggg aagaaaccgc aggtcgcgcc atattccggc gccatattcg
     3361 caatcgtcgc gcggtcggcc aaggaaagat gattaaggcc cgggccataa aactcgacaa
     3421 attttccgac gacgcctttc tgccgaagca tctgcgttac ggtgaggaca aggtcggtcg
     3481 cggtaatgcc ggtgccaagc gcgccagtca atttgaaacc gatcacttcg ggcaacagca
     3541 tggaaagggg ctggccgagc atgcaggctt cggcctcaat gccgccgacg ccccagccca
     3601 gaacggatag gccattgacc attgtcgtgt gcgaatctgt gccgacgagc gtgtcaggat
     3661 aggcaatctc gacggtcgca tgcttgccgc gtactggggc ggatttttcc ttggccgtcc
     3721 acaccgtttg ggccagatat tcgagattga cttggtggca aatgccggtg cccggcggca
     3781 cgacccggaa attgtcgaac gcgtgctgac cccatttgag aaagcggtag cgctcgccat
     3841 tgcggctata ttcgagctcg acattggtct taaacgcctt gctgctgcca aaggcgtcga
     3901 cgatgaccga atgatcgata gcgagatcga cgggcacgag cggattgatc ttttgcggat
     3961 cgccgccgag tttggtcatg gcatcgcgca tcgcggccaa gtcaacaacg gcgggtacgc
     4021 cggtgaagtc ctgcatgagg accctcgctg gacggaacgc aatctcgcgt tcggcggtgc
     4081 ctttattccc cgcccaggca gcgacggctt cgatatccgc tttggtgacg gtgcggccat
     4141 cctcgaaacg cagcagattc tcgagcagaa ttttcatcga gaacggaagc tcggaaatgc
     4201 ccgcaagccc gttcttctca gccgccttga gagagaaata atgataagtt ttggcgccta
     4261 cgttaagctt tttgcggcat ttaaagctgt cgagcgaggt catggaacgt gtcccaggaa
     4321 ggtcgagtgg aaaaggaaat gctgcagcgt ttataaataa gtttttcgcc gggagccata
     4381 tggctgatag atgaaaccgg cggcaatgct tgcccgcggt tcaatcacaa tgcaactttg
     4441 tgcctcaaat ttgacgatcg cgcgcggcgg ccggaccatt atcgcgagcc tgtcctttgc
     4501 gctttgtgct ggcgagtgtc tcgccttgac agggccaaat ggcgcgggaa aatcgactct
     4561 tctgcgcgcc cttgccgggc tattgcctct cgcagctggt acgatctcgc tttcacccct
     4621 atctgaagaa ttcgtgcccc agcaggccca ttacgtgggt catgcagatg gtctaaaggg
     4681 ccttctaacc gttaccgaga atttggagtt ttttgctgcg atccttgacg ttgggatggg
     4741 tggtttgcca gtcaaaaccg cgctcggcaa attgggcctc gcacatgccg ccaagctgcc
     4801 ggtggcctat ctctcggcgg ggcagaagcg acgcgtggca cttgcgaggc ttcttgcggt
     4861 caggcgccca atatggctcc tagacgagcc cttgactgct cttgacacag cctcgcgcgt
     4921 gctgctgggg caaatcatga ccgagcattt gacgcaaggc ggcctgatcg ttgccgccac
     4981 ccatgcgggg ctcgatctga agggccaaga gctgcggctc agggcggccg gatgacaacc
     5041 cccaagggca cgccaaaaga aacgtcgccg cttgccgcgc tttttctccg ggatatcagg
     5101 accgggcggc gcattggcgg cggcacgagc ctcggcatcg tgttttttct aatccttgtt
     5161 acgctctgcg ccttcgcgat cggcccagac cttcggcttc ttgggaagat cgcccccgca
     5221 attctctgga tttcggggct tctttcgacc ttgctcgggc tcgaccggct ttttcaggct
     5281 gatcacgagg acggttcgtt agatctcctt ctcaccagca gcacgccgct cgaactcatc
     5341 atcctcgtca aatgtctggc tcattggatt ctcacaggcc tgccgcttgc cgccacggct
     5401 ccgatctttg gcctcatgct gaacctgcct ctttccgagc tttggagcgt gacgctctct
     5461 cttctggcgg gaaccccttc tcttaccttg accggagcca tcggcgcggc tttgacggtc
     5521 agcctgcggc gcggtggatt attgttcgcg gtattgattt tgccgctctg cgtgccaagc
     5581 ttaatctttg gcgtcgcagc ggctggggcg ggcgaggctt ccttggtccc tttcgcgact
     5641 cccttcctga tcctctgcgc gctcaccctg atcgctgggg ttgttgcgcc cgtggcggcc
     5701 gctgctgcgc tgcggaatgg gggcaggtga aatcttgtgc ttccgcacaa ggtcgcggtt
     5761 tgagcagcgt ccccagccgt ttcttcaaac ccgcagctat gataggtggg acgatccgaa
     5821 acaattaggc ccgcatgcgg gcctaatcta ctcgtgtggc cgtccctgga aaacctccgt
     5881 gatgaacatg aagaacactc tgcttgtcac ttgcatgacc ctgacgctaa tcgtctcaag
     5941 cagtgcttcc tcgcttgcgg gcgagctcac aggtcgcggc gagcaaatga taagcgagac
     6001 cgatccttgt gtgacaaatt gcaagaatgg cgcagattac tgcagagagc aatgctctca
     6061 tcccgaagaa ccggaacagt gcatcgtcgc ctgctcgagg tccgagtgca acgacagttg
     6121 caacaagttt gaggacgcat gcacgaggcg ctgtcaaagt cccaaaggct gatttctgtg
     6181 ctcggggcgc aagtgctggg tcgcgttcat gataaatccc gcggcggccg ggatttttgc
     6241 ggcaggacgt tttgaatggg cctgccgcca cgagccgagc gctttgcgcc gccagctttg
     6301 gtcttcgcag ctttgctcag cgcctgggaa tcctatgtgc ggtttggtga tgtgccgcct
     6361 tatgtcttgc cgtcgccgac gctcattttt tcgaccctca tttccgactg ggcgacgctc
     6421 ttccctgcct tgcttgtgac tttgagaacc actttgggag ccctcgcggc ggcggtcatt
     6481 ggcggagttg gccttgccct tttgttcacg cagtggagat gggtggagcg gtcctttttg
     6541 ccttttgcaa tcgtgctgca agtcaccccg gtggtggcca ttgcgccttt gctgctgatc
     6601 tatcttgacg cacctgcggc ggtgctcgtt tgtgcgttcc tcgtcgcctt ttttcccatt
     6661 ttgtcgagta ctgcgctcgg agtcgcctcg gccgaccgca atcttgtcga tctcttcaag
     6721 ctttatcgcg cgacgcgatg gcaaacgctg ctgcaactgc ggctgccctc ggccctgcct
     6781 tactttctcg gcgggttgcg gatcggcggc ggcttggcgc ttatcggcgc gatcgtcgcc
     6841 gaactcgcgg ctggcaatgc gggcaagggt gcgggcttgg catttcgcat cgccgaggcc
     6901 ggctaccggc tgaacatccc gcgaatgttc gcggcgctca ccctcatcgg gctgaccggc
     6961 atagcgattt atgcagcctt tgctgcatta tcgtcctatc tgcttggccg ctggcatgag
     7021 agcgccgccg ggcccggaaa ctgacgcttc cgcgctcgct cacgcatagt cgaccacgct
     7081 ggccctaaag cgcctcatca aaatgccgtg ttcgttaaaa tatagagaga aaaaccgcgc
     7141 caatttgtca ttaccttgct cgctcttctt aaaaacgggt tgccagtgcg gatcaaaatg
     7201 aagcgcacta gctgcgcgac gagcaattga tatggattgg tgactgttat cggctataaa
     7261 caaatcaatt acgcttattg accatgtttt atctatttgc gacggaggcc tttaaatttt
     7321 cttcacagct tgagttgatt ccataaaagt tttgagcatt attgggactg gccggctacg
     7381 attggtctct ggctcgctag gtcaggatgg cccacttatt gccaagaata cattgcacag
     7441 ctaccagtgt ttttcaccgt tgggtctaaa caatatgttc gtcgacacgc gccagcttga
     7501 agagggatcg gtgatccggt ccgaggtctg cattatcggc ggtggggttg ccgggatctc
     7561 gcttgcgctg gaattcgaaa aacatggcat cgaaacctgc ctgctcgaga gcggagggtt
     7621 caaggcagat gaggccacgc gggatcttta ccgcggggag agcgtcggca ttccttatcg
     7681 atttgccgac ggctgccgag gccgtttcct tggcgggagc agcaactgct ggggaggatg
     7741 gtgccagcca ctgcaggagc atgattttga gcggcgtgaa tgggtgccga acagcggctg
     7801 gccattcgga aaatcagaac tcgagcctta ctacgagcgc agtcacagcc ttctcaagct
     7861 cggtccaaac cgctacgacg tcgatttttg ggtcgatgcg atcggcaaac cgcaaattca
     7921 accgatcgcg ttgaacgcgg cccgggtcgt gaccggaatc tcgcagttca gccctcccgc
     7981 gcgcttcggc acgctttacc gcgacgaact cgcgaaatcc aaaagtaccc gcgtgtatct
     8041 ctacgccaat gccgttgaca ttgaggtcga cgccgatcga gcgatccgct ctgtgcaggt
     8101 gaagacgctg accgggaggg cggttgtcgc cgcggcgaaa atttttgtgc ttgccactgg
     8161 tgggatcgag aatgcgcggc tgctgctcgc ttcgaataag gtccgggcca atggccttgg
     8221 aaatgacaat gatttggttg gccgcttctt catggaccat cctcgcgtct ttacggggaa
     8281 gatccatttc cggaaggggt ggtcaggcca tatgctctac gatgtcttat ttaattacct
     8341 taacaaagca atagccgcgc atggggcctg cgttgcggcg caattcgcgc ttagcccgca
     8401 attgcaggcc aatgagcgcc ttttgaacgc gcgcatatgc ttctcttccc tctttttcgg
     8461 gcaggacacc gaagcccggg aagcgctcgg ccgaatcaaa cacaggtttg cacgggatgc
     8521 cgagtttgat cagagtctcg ctcacgacat ttttgcggtt tcagcccacc ctaccctcct
     8581 ggcacagctt gttctcgccc gcgttttgcg gcgccctcag cttatcaaac attcgcggtt
     8641 ccaggccata gtcgagccgc ttccggatcc tgcgagccgg gtgaccctct cctccgaatg
     8701 cgaccggctt ggggtaaacc gggtgaaggt cgactggcga ctcggcgcac aggtcaagca
     8761 caccttcgac cggaccctcg ccattattgc ggaagagctg acaagcgcag gtgtcgcgga
     8821 tgtggtcctc gacccaccaa tcgaaggtgg ggactggccc agttctttcg agaaggaagg
     8881 gtcttggcac catatgggca cgacccgcat ggacgattcc ccaaagcttg gtgtcgtcga
     8941 caggaaatgc cgcgtacacg ggacgagcaa tctctatgtc gcgggcagtt cggtattccc
     9001 gacagccggg ggagatttcc caaccatcac aatcgtcgcg ttggccctgc gcctatccga
     9061 ccacattgct ggcgaaatgc gccagcccgc cgtgctccgc gggccaatga aggaagcgct
     9121 ccaggcgtag cggatcgtta agctcaaggg taacgccaag ctggtctgcg tcagttgctt
     9181 tcccgctcgc cttgctcgat ccccccgggc ggcctatact cgtggccagc cagggggtgc
     9241 tgccataatg gcggctgaga tcaaacccgt agaacctgaa tctgggtaat gccagcgcag
     9301 ggaccggcta ggcaaaggca agcccagcca gggcccttag ctcccgttcc ccgcttcgct
     9361 ggaaaattcg ccgcggaggt gcgcgatgaa tatttacgaa aagccgcaag aactcgttcc
     9421 gcaaagcgtc acatgcgggc cgctgccggg ctcgagaagg gtctatagcc aagttcaggg
     9481 tcacagcgat atcgccgtgc catttcggga gatcgtgctt gatcccctcg ctgacgaacc
     9541 gccgctaagg gtttatgata cgtctggccc ctacacagag gacggcgttt ccatcgacct
     9601 ttcgaaaggc ctcgccccca ttcgggagcc ttggcttgcc cgccgcgagg ggcttgaaat
     9661 ttattgtggg cgcgcggtgc gtccagagga taatggcggc gtcgcggtcg gccgcctggt
     9721 gccgccttgc ccagcgaaca ggttaccaca caagggctgc caggacgccc cggtaactca
     9781 atatgaattt gctcgggcgg gcatcatcac tgaggagatg atctttgccg cggcgaggga
     9841 aaatcttggc cgcgaaaaaa tagctgacga t
//
LOCUS       DATMPU010000300         3209 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_1774770,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000300 DATMPU010000000
VERSION     DATMPU010000300.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 3209)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3209)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3209
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1774770"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..525
                     /locus_tag="VNY06_06860"
     CDS             <1..525
                     /locus_tag="VNY06_06860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018640959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HWX14557.1"
                     /translation="SMGEIARSAGVSKGTLYVYFADKSRLFEAIVEEEALEKGQVEFD
                     LSPERDAAITLLEFGEAYIAMLCRPGGGSAIRTVMAIAERMPEVGRRYYGKVLERTID
                     RLAAYLEARVKANELAIDDCQHAASQFMLMCQASLFLPFVFQAAPPPSAARIREVVES
                     ATRVFLAAYGMKAD"
     gene            571..903
                     /locus_tag="VNY06_06865"
     CDS             571..903
                     /locus_tag="VNY06_06865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX14558.1"
                     /translation="MSRPSTFLARLRRQDVDARHKAGHDEPRVDRRRAIPNSRFSFQT
                     TEAVIARSESDEAIHLSLRDDMDCFAEPVISARALLRSSGARIRATRWLAMRQGDGFR
                     YGLISSTA"
     gene            1587..2606
                     /locus_tag="VNY06_06870"
     CDS             1587..2606
                     /locus_tag="VNY06_06870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016847229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX14559.1"
                     /translation="MPPAMGQPIAPAKKQSDALNTYNNAIGDFKSILAERRRQINSHQ
                     RLPNLPGQALYLARINVMSAYKDLTDAVPSKIGRPNKFGIPPAYFDADNEPLVDEYRS
                     LFDLLEAPPANAQDSAAPFKDVVDLGIAVARAKGLDATSAEAAGRISLGLFFAETNGH
                     QNIGNGRSNTYKGSFQTGPSEDQNGRRKWQAIKRSIAAFDPELIARDDKEEARVGDLD
                     HRFNHWTAVRDGLMNAHADLFPQIPAIVKTLPNPIDQMKVFELIQIIPSPTRSALKSG
                     SLLDYRISDPRIMGYLRNNSVFAFGQADRARTSASFRDILDAMWLFNQKFEQALSKFR
                     EIKAR"
     gene            complement(2678..>3209)
                     /locus_tag="VNY06_06875"
     CDS             complement(2678..>3209)
                     /locus_tag="VNY06_06875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008968594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX14560.1"
                     /translation="IVLAECCSWHAVLTTNFLGNAIENSIWAVAFLVVGIGLCRLLPE
                     FNGPVRVALVIAISGIASYLAFLMIIDVPMYLSRWRAGLADGSKLLGHLEGLRDVSTR
                     WVVTHDIAHWKGEIAWMSLYFSAAVWASLALCVLYSLEDHLPRYRTEAAVASFSSDAL
                     AVAGKQPSRERPSAAM"
BASE COUNT          728 a          932 c          943 g          606 t
ORIGIN      
        1 agcatgggcg agatcgcccg ctccgccggc gtttccaagg ggacgctcta cgtctatttc
       61 gccgacaaga gccggctgtt cgaggccatc gtcgaggaag aggcgcttga aaagggccag
      121 gtcgagttcg atttgagccc cgagcgcgac gccgccatca cccttctgga attcggcgag
      181 gcttatattg cgatgttgtg ccgtccgggc ggcggatcgg cgatccgcac cgtgatggcg
      241 attgcagaaa ggatgccgga ggtcggccgc cgctattacg gaaaggtgct ggagaggacc
      301 atcgaccgct tggccgctta cctcgaggca cgggtgaaag cgaatgagct tgcgatcgac
      361 gattgccagc acgcggcatc gcaattcatg ctgatgtgcc aggcgtcgtt gttcctcccc
      421 tttgtgtttc aggccgcccc gccgccctcg gccgcgcgca tccgcgaagt ggtcgaaagc
      481 gcgacgcggg tgtttttggc ggcgtatggg atgaaggcgg attgaggcgg acggtgcgga
      541 ctctggtctc gtctttcgtc atggccgggc ttgtcccggc catccacgtt cttggcgcgg
      601 ctacgaagac aggacgtgga tgcccggcac aaggccgggc atgacgagcc tagggtcgat
      661 cgccggcgtg caatcccaaa ttcccgattc agttttcaaa caactgaggc cgtcattgcg
      721 aggagcgaaa gcgacgaagc aatccatctt tccttacgcg acgacatgga ttgcttcgcg
      781 gagcctgtca tctctgcgcg agcgctattg cgctcgtcgg gggcgcgcat tcgcgcgacc
      841 cgttggctcg caatgcggca aggcgatggg tttcgctacg ggcttatcag tagcaccgcg
      901 tgatattgac ggtttgctcg gtgccgtaac gcccgggaac cgttacggtc tccgcggagc
      961 agctcgagac atagggccgg tcagaaggtg tgacagccgg cgggaatcga tgtgcccaat
     1021 cccagggaat atcgtatgtc tgcgtataat ggatatcgcc tgatgttggt tgcgcgacat
     1081 ttgccgtggg ctcaccgtac gatggcccgt aggagtagtc cgctacgccc ggccagaagg
     1141 ccccgacatt atttctcctg tgatgccgga gcgaatgggc gaggaacgga cgggacatcg
     1201 aaggcgtcga agcaaaccga ggcgctgctc ccgagcgagc cgatgtctcg ttcgcgccaa
     1261 gcatcagcgc gatcgcgctg agagacacga taaacgccgc attcattcta tacgccatgg
     1321 cacgcaccaa ttcgttggtt actacacagc accccaacgg acgctaggcc gggcctttag
     1381 cgcccacaaa tgcataatta attattagtt aaagcgtaac attaataggg agccattttc
     1441 actaaagaac gaaatggcca agagattcat tggcttagcc ggtgaaacca caggcatttg
     1501 gaaacaccat ggggatacga agtcatacaa ccaaggcaac tcttgcgctc gcaatcttcc
     1561 tcacgccgcc ggccaatctc agcttcatgc cccctgcaat ggggcagccg atcgcgcctg
     1621 cgaaaaagca atcggatgca ctcaatacct acaataacgc aataggcgat ttcaaatcga
     1681 tcctggccga gcggcgccgc cagatcaatt cgcatcagcg gcttccgaac ttgccgggac
     1741 aagccctcta cctcgcacgc atcaacgtca tgagtgccta caaggatctc accgatgccg
     1801 ttccgtccaa gatcggaagg cccaataaat ttggaattcc tccggcgtat tttgatgccg
     1861 acaacgaacc gctggtcgat gagtacagga gcctgttcga cctcctggag gcgccacccg
     1921 ccaacgcgca agattcagct gctcccttca aggacgttgt cgatttgggc attgcggtcg
     1981 cgcgagccaa gggccttgat gcgaccagcg ctgaagcggc agggcgtatc agcctgggac
     2041 tgttttttgc cgagacgaat ggccatcaaa atatagggaa tgggcgctcg aatacctaca
     2101 agggcagttt tcaaacggga ccgtccgagg atcaaaatgg tcgaaggaag tggcaagcaa
     2161 taaaaagatc gatcgcggcc tttgatcccg aactcatcgc gcgcgacgac aaggaagagg
     2221 cccgggtcgg cgatctggat catcggttca accactggac cgccgtgaga gatggcctga
     2281 tgaacgcaca cgcagacctt ttcccgcaaa taccggcgat cgtgaaaacc ctgccaaacc
     2341 cgatcgatca aatgaaggtt tttgagctca tacaaatcat tcctagtccg acaagatccg
     2401 cgctcaagtc aggcagtctg ctcgactaca ggatttccga ccccaggatc atgggatatc
     2461 tgcggaacaa cagcgtcttc gcctttgggc aggctgacag ggcaagaaca tcggcaagtt
     2521 tccgcgacat ccttgatgcg atgtggttgt tcaatcagaa attcgaacaa gcgctttcga
     2581 aatttcgaga gatcaaggcc cggtgaaagg ttggttgagc ggtcagcttg accccgggca
     2641 gcctacacaa gagcggactc cgcaagggag gcctgcctca catcgcggcc gacgggcgct
     2701 cccttgaggg ctgcttgcca gccactgcga gcgcgtccga cgagaagctt gcgaccgccg
     2761 cttcggtacg atagcgcggg agatgatcct cgagcgagta gagaacgcaa agcgcgaggc
     2821 tagcccagac cgccgcactg aaataaagcg acatccaggc aatctcgccc ttccagtgag
     2881 caatgtcgtg cgtcacgacc caacgcgtgc tcacgtcacg aaggccctcg agatgtccca
     2941 aaagcttgct gccatcggcg aggccagccc gccatcggct cagatacatc ggaacgtcga
     3001 tgatcatgag aaacgccaga tagcttgcaa tccccgatat cgcgatgacg agggccacgc
     3061 gaaccgggcc gttgaactcc ggcagcaacc ggcagaggcc gatcccgacc acgaggaagg
     3121 cgaccgccca aatcgaattt tcgatggcgt tgcccaggaa attggtggtc agcaccgcat
     3181 gccatgagca gcactccgcc aggacgatc
//
LOCUS       DATMPU010000301         5544 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_1776670,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000301 DATMPU010000000
VERSION     DATMPU010000301.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 5544)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5544)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5544
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1776670"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1137
                     /gene="thrC"
                     /locus_tag="VNY06_06880"
     CDS             <1..1137
                     /gene="thrC"
                     /locus_tag="VNY06_06880"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018045006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HWX14561.1"
                     /translation="DSDLVVLELFRGPTLAFKDLAMQLLARFIDHVLKTRGERATIVG
                     ATSGDTGAAAIEAFAGLSQVDVFILYPHGRVSEVQRRQMTTAEAGNIHTIALEGTFDD
                     AQMCIKNMFNNRVLRDELSLCGVNSINFARILFQTVYYFTSAMVLGAPHRPLSYVVPT
                     GNLGDIFAGWMAKRMGLPIDRLAIATNSNDILARTLASGCYAVERVLATQSPSMDIQV
                     SSNFERLLFEVHGRDPATLCGLMEGLRQSQRFTIAPGPLEAIRAEFDAFSVTEEETTQ
                     AIADTWRDTGYLVDPHTAVGLSAARKARAKDSGYPCIVLGTAHPAKFPDAILRATGLQ
                     PKLPCHLADLFERAECHSVLPNEQSTIEEFVRSRATRGSNSAAS"
     gene            1134..2399
                     /locus_tag="VNY06_06885"
     CDS             1134..2399
                     /locus_tag="VNY06_06885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020176685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HWX14562.1"
                     /translation="MSVEITTLPSGLRIVTDRMAHLETASIGVFVGAGSRHEQVSEHG
                     LSHLLEHMAFKGTRRRSARDIAEEIEMVGGDLNAATSTEHTAYFAHVLAGDVPLALDI
                     LADVLTESTFELPELEREKDVILQEIRAIGDTPDDLVFEMLNSAAFPDQPIGRPILGT
                     AERVSGFAWDALRTYLDTHYRAGAIVIGAAGAVEHDSFRDEVAHRFSGLPSVCVETDT
                     DPAQYKGGDKRLKRHLEQVHIAVGFEGIRYTHEDFYALQLFTNAAGGSMSSRLFQEVR
                     EARGLAYSITAFQWAYSDSGVFGFYAATDAKNSDELMRVALDSLGKAALDLSDIEVKR
                     AKAQLKVSLLTALEAPGARCEQIARQIMAFDRILTRDEIMAMVDRLDAGQIRAAGARA
                     LGSAPTVAAIGPVAKVFTPDRVTMRLQGI"
     gene            2411..3010
                     /locus_tag="VNY06_06890"
     CDS             2411..3010
                     /locus_tag="VNY06_06890"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018266726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HWX14563.1"
                     /translation="MALFRLSPVSQTEILLRGEGVYLRPPEMRDYESWSDLRAASRPF
                     LIPWEPTWPGDDLTRGAFRRRLRRSHQEIANDEAYPFLIFRDNGVLVGGLTLGQVKRG
                     VVQSGTLGYWIGALHARQGLMSAAVRAVIGYGFTTLRLHRIEASCLPHNRASIRLLER
                     TGFTKEGYARAYLRINGVWQDHLLYGLLDSDASPLHLKD"
     gene            complement(3038..3283)
                     /locus_tag="VNY06_06895"
     CDS             complement(3038..3283)
                     /locus_tag="VNY06_06895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX14564.1"
                     /translation="MFDNLPPLRIAAVIYFAVFDLVAAVGLWLATPWGGVIGYLVRSH
                     KSLSPLHCPVFSIFCVDLEGGHAGAALVKLRHWRNKP"
     gene            complement(3471..4532)
                     /locus_tag="VNY06_06900"
     CDS             complement(3471..4532)
                     /locus_tag="VNY06_06900"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012589943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HWX14565.1"
                     /translation="MNEAEVICEKVGCCGRITLNRPKALNALTLNMVREIAAALDRWE
                     SDPAVACVIITGAGGNAFCAGGDIRVLYNLGKAGRYAEQLCFWREEYRLNRRIKFYPK
                     PYLALADGIVMGGGSGVSLHGTYVIAGENFTFAMPEVGIGFVPDVGATFFLPRLPSRA
                     GIYLALTGARMTCGDALAFKVASTHVPSARHTGFIERVVRGEAIEAAIAAEHAPPPAS
                     ALFKESELIARCFAAETLPAILGNLELAARANSSFAHSTACAIRAKSPTSLAITLRQM
                     QIGATMNIGEALQTEFRIASRIAKGNDYYEGVRAVIIDKDNRPHWTPATIESVPHADI
                     DAYFAPLPGGELDFGTPAC"
     gene            complement(4621..5526)
                     /locus_tag="VNY06_06905"
     CDS             complement(4621..5526)
                     /locus_tag="VNY06_06905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020175509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity EipB family protein"
                     /protein_id="HWX14566.1"
                     /translation="MIMGITCFRFWFAALPAAALAVVGVAAGMTFPANSESATPDPLP
                     LAAANVGNEVALVSHRAVYELTLLKSTGTKSPTAAHGRIAFDFTGSPCDGYVQNFRQL
                     TELQPAEGPTRVSDMHSATFEDADGRNFEFKMQTRIDSGAAEQVDGKARRSAGGLLSV
                     SLIKPKHERVELDRNVVFPTEHLRRILAAAEAGKNLLELSVFDGSDTGEKVYDTTTYI
                     GHPVAGPAVEKAAHIPELDNLKRWPVSISYFETGNKDDAPSYILSFDLYENGISRALR
                     LDYGDFVLAGEMSSLELLRTPTCTK"
BASE COUNT         1197 a         1535 c         1586 g         1226 t
ORIGIN      
        1 gattcagatc tcgttgtgct tgaattgttt cgcggtccga ccttagcttt taaggacctc
       61 gcgatgcagc ttcttgcaag gttcatcgac catgttctga aaacgcgcgg tgagcgggca
      121 accatcgttg gcgcaacttc gggcgatacg ggagccgcgg cgatcgaagc ctttgctggg
      181 ctttcccaag tcgatgtttt tattctttac ccgcatggcc gggtgtcgga ggtgcagcgc
      241 cgacaaatga ccactgctga ggccggaaat atacacacga ttgcgttgga gggtactttt
      301 gacgacgcgc aaatgtgcat taaaaatatg tttaataaca gagtattacg tgacgaactt
      361 agcctttgcg gagtgaattc gatcaatttt gcgcggatcc ttttccagac ggtctattac
      421 ttcaccagtg ccatggtcct cggtgcaccg catcggccac tttcctatgt cgtgccgacc
      481 gggaatctcg gcgacatttt cgccggctgg atggccaagc gcatgggact tccgatcgac
      541 aggctagcga ttgcaacaaa ttccaatgat attttggccc gcaccctggc gagcggctgc
      601 tatgccgtag agcgggtcct tgcaacccaa tcgccctcca tggatattca ggtgtcatct
      661 aatttcgaac ggctcctttt tgaagtccat gggcgtgatc cagctacgct ttgcggcttg
      721 atggaggggc tgcggcaatc gcaacgtttc accatcgctc ccggcccgct tgaggctatt
      781 cgtgcagagt tcgatgcgtt cagcgttaca gaagaagaga ccacgcaagc cattgcggat
      841 acttggcgtg atactggcta cctcgtagat ccccacacag ctgtcggatt gagtgccgcc
      901 cggaaggcga gagcgaagga tagtggctac ccgtgcattg tgttaggcac cgcccatccc
      961 gccaaatttc ccgacgcgat tttgcgcgca acagggctgc agccgaaatt gccttgccat
     1021 cttgcagacc tgttcgaacg agcggagtgc cactcggttc tacccaatga gcagagcaca
     1081 atcgaagagt ttgtccgttc ccgcgccaca cgtggttcaa atagtgctgc gtcatgagcg
     1141 tcgaaattac aactttgcca tcgggtctcc ggattgtcac cgatcgcatg gcgcatctcg
     1201 aaaccgcctc cattggcgtt tttgtcggcg caggatcgcg tcatgagcag gtgagcgagc
     1261 atggcttgtc gcaccttctc gagcatatgg cgttcaaggg aacgcgtcgg cgctcggccc
     1321 gcgacatagc cgaggagatt gaaatggttg gcggcgatct caacgccgca acgagcaccg
     1381 aacatacagc ctattttgct cacgttttgg cgggagatgt tccgctcgcg ctcgacatat
     1441 tggccgacgt tttgacggaa agcacatttg aattgccgga gctcgaacgg gaaaaggacg
     1501 ttatcctgca ggagatccgc gccatcgggg acacccctga tgatctcgtc ttcgagatgc
     1561 tgaactccgc ggctttcccc gatcagccga tcgggcggcc aatccttgga accgctgagc
     1621 gagtctcggg gttcgcctgg gatgccctaa ggacctatct cgatacgcat tatcgggccg
     1681 gagcaatcgt gatcggcgcg gcgggagctg ttgagcacga ttccttccgc gacgaggttg
     1741 cacaccgctt ttcgggactt ccctcggttt gcgtcgaaac cgacacggat cctgcgcaat
     1801 acaaaggcgg cgacaagcgg ttgaaacggc acctcgaaca ggttcacatc gcggttgggt
     1861 tcgaaggtat tcgttacacg cacgaggatt tttatgccct ccagcttttc accaatgcgg
     1921 ccgggggcag catgtcttca cgcctgttcc aggaggtgcg tgaggcgcgt gggctggcct
     1981 attccatcac tgcctttcaa tgggcatatt cagacagcgg cgtgtttggg ttttatgccg
     2041 cgaccgatgc aaaaaatagc gatgaattga tgcgcgtggc gttggacagt cttggcaaag
     2101 ccgcgttgga cttgagcgat atcgaagtaa agcgggcgaa ggctcaattg aaggtttcgc
     2161 tgcttaccgc gctagaggct cccggtgccc ggtgcgaaca gatcgcgcgg caaataatgg
     2221 cgtttgaccg gatactcacc cgggacgaaa tcatggcaat ggttgatcgc ctggacgctg
     2281 ggcaaatccg tgctgccggc gcacgcgcgc tcgggtcagc accgacagtt gcggcaatcg
     2341 gtccggtagc caaggttttt acgccagatc gtgttaccat gcggcttcag gggatttagg
     2401 ggtcagtatc gtggcgcttt tccggctgtc tccagtcagc caaacagaaa tactgctgcg
     2461 tggcgaaggt gtttatctcc ggccgcccga gatgcgtgac tatgaatcct ggtcggatct
     2521 acgtgcagca agtcgtccgt tcttgattcc gtgggagccg acctggcctg gggacgatct
     2581 cactcgcgga gctttccggc gacgtctgcg ccgcagccat caggagatcg ccaatgacga
     2641 agcctaccca ttcttgattt ttcgcgacaa tggcgtcctt gtcggcggac ttactctcgg
     2701 acaggtcaag cgcggcgtcg tgcagtctgg gacgctggga tattggatcg gagcactcca
     2761 tgcccggcaa gggctgatgt cggctgcagt ccgggccgtc ataggctatg ggtttaccac
     2821 tcttcggctg caccggatcg aagcttcgtg cctgccccac aaccgggcat cgataagatt
     2881 attggagcgg acgggattta ccaaggaggg ctatgcccgc gcctatttgc gaataaatgg
     2941 ggtctggcag gaccacttgc tctatggttt gctagactcc gatgcatcgc cgcttcacct
     3001 caaggattga cagtcggctt gcccgggccg ggacagtcta tggcttatta cgccagtgac
     3061 gcagtttgac caaagccgcg cccgcatgcc cgccttccag gtcaacacaa aatattgaaa
     3121 aaaccgggca gtgcaatggc gacaaagatt tgtgtgagcg caccaaatag ccaatcacac
     3181 cgccccacgg cgtcgccagc cacaatccaa ctgcggcaac aaggtcaaat accgcgaagt
     3241 agatcaccgc ggcgatgcgc agcggcggta gattgtcgaa caaaggctct gagggcagaa
     3301 gaatagcact ccattggacc acagccctgc aaggagacgt aagaatacta ccgaaattag
     3361 gcctgcgcga tagctgtcct tgagggcgag cgcgccgtcc ctaagagaaa tggcacacgc
     3421 gagatcgcga ggtgctgcag gctcgagtga ttcggggagc atagtcatgt tcaacatgcg
     3481 ggcgtgccaa aatcaagttc gcccccagga agaggtgcaa agtaggcatc aatgtcggcg
     3541 tggggaacgc tttctattgt cgcaggcgtc caatgcggtc gattgtcctt gtcgataatc
     3601 acggcgcgga cgccttcata gtagtcattg ccttttgcaa tgcgcgaggc gatgcgaaat
     3661 tcagtttgca aagcctctcc aatattcatt gtcgcgccaa tttgcatttg acgcaacgtg
     3721 atggcaagac tagttggcga ttttgcgcgt atcgcacatg cggtcgaatg tgcgaacgac
     3781 gagttggcac gcgcggctaa ttcgagattg ccaagaatgg caggcagggt ctcagcggca
     3841 aaacagcgtg caataagctc ggactctttg aaaagcgccg aggcgggcgg cggcgcatgt
     3901 tctgccgcga ttgcggcctc gatcgcctcg cccctcacaa ccctttctat aaaacccgtg
     3961 tggcgcgccg agggcacatg agttgaggcg actttgaatg ccagcgcatc gccgcaggtc
     4021 atccgtgcac ctgtcaaggc gagatatatg ccggcccgag aaggtaagcg gggcaagaaa
     4081 aaagttgcgc caacatcggg gacaaaccca attccgactt ctggcatggc gaaggtaaaa
     4141 ttctcgccag cgatgacata ggtcccatgc agagaaaccc cggagccgcc tcccatcaca
     4201 attccgtccg cgagggcgag atagggttta gggtagaatt ttatgcggcg attgagacga
     4261 tattcctcgc gccaaaagca aagttgttct gcatagcgcc cggccttgcc gagattatac
     4321 aagactctga tgtcgccgcc cgcgcagaaa gcgttgcccc cagctcccgt tataatgaca
     4381 caggcgactg ccggatcgct ctcccagcga tcgagcgcgg ctgcgatctc ccgcaccata
     4441 ttcaaggtta gcgcgttgag tgccttcggg cggttaagcg ttattcgtcc gcaacagccc
     4501 actttttcac agataacttc cgcttcgttc atgacctctc aaatgccaaa gctccgagca
     4561 ttggctggag ataagatgac atgtcgcccc cgagatacct gccgcgatga tccgaacgct
     4621 ttatttggtg caggtaggcg tgcgaagcag ctcaagactg gacatttccc ccgcaagaac
     4681 gaaatcgccg tagtcgagtc tgagcgcgcg cgaaataccg ttctcgtaaa ggtcgaaaga
     4741 gagaatgtag ctcggcgcgt catccttgtt accagtctca aaatagctga tggaaaccgg
     4801 ccagcgtttc aaattgtcga gttcggggat atgagcagcc ttctccacgg cggggccggc
     4861 aaccggatgc ccgatatagg tggttgtgtc ataaactttt tcgcctgtgt cagaaccgtc
     4921 aaaaacactc agttcgagca agttcttacc tgcctcggct gctgccaaga tgcgcctcaa
     4981 atgttcggtt gggaaaacca cgttccgatc gagttcgacc ctctcgtgtt tcggcttgat
     5041 gaggcttacc gatagcaatc ctccggcgct cctccgagct tttccatcga cctgctccgc
     5101 ggcgccgctg tctatcctcg tttgcatctt gaattcaaaa ttccggccat ccgcgtcttc
     5161 gaatgtcgcc gagtgcatat ccgagactcg agtaggacct tcggcgggct gcaattcggt
     5221 taattgacgg aaattttgca catatccgtc gcatggggag ccggtgaaat cgaaggcaat
     5281 gcgtccgtgc gcggcggtcg gactcttcgt tccggtcgat tttagcagtg tcagttcgta
     5341 gaccgcgcga tgcgatacaa gtgcaacctc atttccgaca ttcgctgcag ccaatggtaa
     5401 gggatcaggg gtagccgact ccgaatttgc cgggaaggtc atgcccgccg cgacgccaac
     5461 caccgccagg gctgctgctg gaagcgcagc gaaccaaaac ctgaagcaag ttatgcccat
     5521 tatcattggt tcctcgaaag tcgc
//
LOCUS       DATMPU010000302         4173 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_2105729,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000302 DATMPU010000000
VERSION     DATMPU010000302.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 4173)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4173)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4173
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2105729"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..475)
                     /locus_tag="VNY06_06910"
     CDS             complement(<1..475)
                     /locus_tag="VNY06_06910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX14567.1"
                     /translation="MHDDEVSRRKGLTFAQAEGLSPLPAQLSRTEVTPELRAVLWKFI
                     HDEIGRSTRGDYLGAVWEKILRNVHVLHYHRFTDDFSTHLKTVTQAVKSVRSVFEQGN
                     YADVYGWLQFVLQHQPPPGFARQLEDRLRYCRAPYRILADDVLCPIGSDLDAAVVQ"
     gene            complement(549..623)
                     /locus_tag="VNY06_06915"
     tRNA            complement(549..623)
                     /locus_tag="VNY06_06915"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(588..590),aa:Glu,seq:ttc)
     gene            947..1204
                     /locus_tag="VNY06_06920"
     CDS             947..1204
                     /locus_tag="VNY06_06920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011664335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HWX14568.1"
                     /translation="MIAANKKREARKSLHQPGWVTLDGGFAARQCVVQDLSSSGAKIT
                     VDDASALTGRLRLAFSRDARTGRRCEVVWRRGKSLGVKFVR"
     gene            complement(1232..1879)
                     /locus_tag="VNY06_06925"
     CDS             complement(1232..1879)
                     /locus_tag="VNY06_06925"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012044101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043295 - glutathione binding [Evidence
                     IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HWX14569.1"
                     /translation="MPNNNQGQLSIWGRANSVNVQKVLWCLRELDLGYERIDAGMAFG
                     RNRDADYLAMNPNGRVPTLVDGDFVLWESNSVLRYLVLAYRPQSPLYPQAPKQRAGVD
                     RWLDWTLSTLQPVDRPVFWALVRTPVEQRDMVAIQRDADAEAAQWRIVDAALATRRFI
                     EGDDFTLADIALGAYARRWFGVEGISKPPLAHLERWFAQFAGRPGFAQFIAPPMS"
     gene            complement(2240..2689)
                     /locus_tag="VNY06_06930"
     CDS             complement(2240..2689)
                     /locus_tag="VNY06_06930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018641785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HWX14570.1"
                     /translation="MRYLHTMLRVRNLDAALKFYQDALGLKEVRRIDNDKAKFTLVFL
                     CAAEDEGLLKASKGRGAPLVELTYNWDEEKYGEDRYFGHLAYEVDDIYATCERLMKLG
                     ITINRPPRDGQMAFVRSPDLHSIELLQKGDAKPPAEPWTSMPNTGHW"
     gene            2870..3013
                     /locus_tag="VNY06_06935"
     CDS             2870..3013
                     /locus_tag="VNY06_06935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX14571.1"
                     /translation="MRGGTNVERLRQGAKDITEYTERTLRLFRKIYAEQELEHEETQR
                     CGN"
     gene            complement(3094..3945)
                     /locus_tag="VNY06_06940"
     CDS             complement(3094..3945)
                     /locus_tag="VNY06_06940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX14572.1"
                     /translation="MAEKDCRFITNRGMSDSPSSAAGEIVYETLVLPVMAHFPEFATH
                     LFEYDIEPGSIADDFMGTVLEADLVIADMTDLSYSGYFQLGVRHNSGRPMVLIADEAL
                     MLSSEPANFRIVRYPYETPAPSGEEKRATSELIEAIKDALADERRSSGLSLLKRQSPK
                     QTRAQLASRLYEAAEAIRLLRLNSTSDAVLELESIAKDLEEVPEDKIAPALQETAIKF
                     LKILSRLADQLATVRGARMLISGVISVVLGGAGFSAITAFGISLAFWEGKEAFLKAIE
                     FLSKRKK"
BASE COUNT          930 a         1166 c         1201 g          876 t
ORIGIN      
        1 gttggaccac cgccgcgtcg agatcggaac cgatcggaca aaggacgtcg tccgcaagga
       61 tgcgataggg cgcgcggcaa tatcggagcc ggtcctcgag ctgcctcgcg aaccccggag
      121 gcggctggtg ctgaagcacg aactgaagcc acccgtagac gtcagcgtag ttgccctgtt
      181 caaagacact tctcactgat ttcacagcct gggtcacggt ttttaggtgt gtcgaaaagt
      241 catctgtgaa gcggtgatag tgtaggacgt gcacgtttcg caaaatcttt tcccaaactg
      301 cgccgaggta atctccccgc gtggatcgcc cgatttcgtc atgaatgaac ttccagagca
      361 ccgcgcgaag ctctggggtg acttccgttc tcgaaagctg agcgggaagc ggcgacagcc
      421 cttctgcttg ggcaaaggtc agcccctttc ggcgagaaac ctcatcatcg tgcataggcg
      481 tctataccca tatattggac acctattgga caccggggcg ccgaatggag ctaagccatt
      541 gaaaatattg gcgctcccta gcggaatcga accgctctct ccaccgtgaa agggtggcgt
      601 cctaaccgat agacgaaggg agcgaaaacc ttggcttcgc catgaccgac ccggtcgcgg
      661 catcgccgtt tcgcactgtc gaaagacaaa tatacccggc aaatcaacgc tttgaggctg
      721 cgatggcgcc gtgccgagcc cgggtcaggg cgaattgagc ctgagcgcga cgggcgaacg
      781 tatagtggcg tttgcgcggc cgggcaagcc gcccggaaac cggttttccc ggcaaaatga
      841 agctgtcgca gcagcgccgg tgccgtcaat tttcgggggc cttgcaacag ctttttaagc
      901 ctaacggcct agggctgtag cgggaatatt gtccaccgga agcgaaatga ttgcagcaaa
      961 caaaaagcgc gaggcgcgca aatcgctaca ccagccgggg tgggtcaccc tcgatggcgg
     1021 ctttgcggct cgccaatgcg tggtgcagga tttgtccagt tcaggtgcga agatcacggt
     1081 cgacgatgcc agcgcgctga ccggcaggct gcggcttgcc ttctcgcgcg acgccagaac
     1141 ggggcgcaga tgcgaagtgg tctggcgccg cggaaaatcg ctcggcgtca aattcgtccg
     1201 gtaatttccg acatcatttc acgacttcac ttcacgacat cggcggcgcg atgaattgcg
     1261 caaatcccgg ccggccggcg aattgcgcga accagcgttc gagatgcgcc agcggcggtt
     1321 tgctgatgcc ctcgacgccg aaccagcgcc gcgcataggc gcccagtgcg atatcggcga
     1381 gtgtaaagtc atcgccctcg atgaaacgcc gcgtcgccag cgccgcgtcg acgatgcgcc
     1441 attgcgcagc ctcggcgtcg gcatccctct ggatcgcgac catgtcgcgt tgctcgaccg
     1501 gggttcgcac cagcgcccag aacaccggac gatcgaccgg ctgcagcgtc gagagcgtcc
     1561 agtcgagcca gcggtcgacg ccggcgcgct gtttcggcgc ctgcgggtaa agcggcgatt
     1621 gcggccggta ggcgaggacg aggtagcgca agaccgaatt ggattcccac aacacgaaat
     1681 ccccatccac cagggtcggc acgcgtccgt tcggattcat cgcgagatag tcggcatcgc
     1741 ggttgcggcc gaatgccatg ccggcatcga tgcgctcata gccgagatcg agttcgcgca
     1801 ggcaccacag cactttttga acgttgaccg aattggcccg gccccagata ctgagttgac
     1861 cctgattgtt gttcggcacc tgttatcgct cccgccattg atcgctgtgt gatccatgcg
     1921 ggtcatatcg gagttttgga aaaaaagcgc gtgtgccgtg cgatgccgcg tcaaaaaaga
     1981 tgtcgcgtta aaaagataag gctccgccat cggcggagcc ttatttacgt ccggtaacaa
     2041 ctatcgcctg atatcagcgg cctaagaaca accacaacaa aatgatcacc ggaataggca
     2101 cgcccaaaag ccagagcaaa attcctcgtc ccattatcgc ctccttgaaa tcttgattga
     2161 ttcaagaacg ccggaggcaa tgcgatgttc cagtgaaagt catggaaaac gaaatcgcac
     2221 cagggatatt tgaacggtcc taccagtgac cggtgttcgg catcgacgtc cacggctctg
     2281 ccggcggctt cgcatcgccc ttttgcaaca gctcgatcga atgcagatcc ggtgagcgga
     2341 cgaaagccat ctgcccgtcg cgcggcggcc gattgatggt gatgccaagc ttcatcagcc
     2401 gctcgcaggt cgcatagatg tcgtcgacct cgtaggcgag gtggccgaaa tagcgatcct
     2461 cgccgtattt ctcctcgtcc caattatacg tgagttcgac cagcggcgcg ccgcgcccct
     2521 tggaggcctt caacagtcct tcgtcctcgg cggcgcacag gaacaccagt gtgaactttg
     2581 ccttgtcgtt gtcgatgcga cggacttcct tcaatcccag tgcgtcctgg tagaatttca
     2641 gcgcagcatc gaggttgcgc acgcgcagca tggtgtggag ataacgcatg ttttctgact
     2701 ccttcagaga tcgagcaagg gaatgagatt gggttcgggg agttcaatac cagtaaaatg
     2761 cccgcgaggg cagggcgttg caaggcaacg cctgcgaaca atcccgcttt cacgcatttc
     2821 aacgctgctt acgcgaaaag gtgactccag gtcagggcgg tttcagctca tgcgaggcgg
     2881 tacgaatgtc gagcgcctgc gtcagggcgc taaggacatc acggaatata cggaaagaac
     2941 gctgcggctg tttcgtaaaa tctacgccga gcaggagttg gagcacgagg aaacgcagcg
     3001 gtgcggtaac tgattattag gaatcgtccg gcgctgattt gcgcgattcc gattcgttct
     3061 ttgagaacgg aatagagtca gagagggaac agactacttc tttcgcttgc tcaaaaattc
     3121 gattgcttta aggaaagcct ccttaccctc ccaaaatgcc aagcttattc caaatgcggt
     3181 gatggcagaa aacccagcgc ctccaagaac gactgagatg acgccggaaa tgagcatcct
     3241 tgctcctcgg acggtcgcga gttggtctgc taaccgggaa agtattttta ggaatttgat
     3301 agcggtttcc tgaagagctg gagctatctt atcttcggga acctcttcaa gatcttttgc
     3361 aatgctttcg agttctaaaa ccgcgtcaga ggtcgagttt aatcgaagta ggcgaatagc
     3421 ttccgctgcc tcgtaaagcc tcgatgctag ctgcgcgcgg gtttgtttgg gcgattgtct
     3481 ttttagcagg cttaagccgg acgagcgccg ctcgtcagct agcgcgtctt tgatcgcttc
     3541 aattaactcg gaagtggccc gcttctcctc gccggaaggc gctggggtct cgtaagggta
     3601 acgcacaatt cgaaagttgg ccggctccga tgaaagcatc aatgcttcgt cagcaatgag
     3661 aaccattggg cgtccggaat tatgccttac gcctagttga aaatacccgc tgtacgaaag
     3721 gtcagtcata tcggcgatga ctagatcagc ttcgagaacg gttcccatga agtcgtcagc
     3781 tatcgaaccc ggctcaatgt cgtattcaaa aagatgggtc gcaaattcag ggaagtgcgc
     3841 catgaccggc aagaccaacg tctcatagac aatctcgcca gcagcgctcg aagggctgtc
     3901 tgacatgcct cgattggtga taaatcgaca gtctttttcg gccattgctc acctaatcaa
     3961 aaaggccccg aagggcctaa cgcggagcgt gtattattcc agaccttcgc cttcccggca
     4021 atctatctcg atgcagatga tcgctgtggg ctcgagctta gtgcttcaag atcggcattg
     4081 tcccgcgtct cgtgcaattg ctcgctgtca aatcaggata gatattcagc cagcccttga
     4141 tgcgctgcgt cttcatctgg tcggcaacga agg
//
LOCUS       DATMPU010000303         4954 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_224228,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000303 DATMPU010000000
VERSION     DATMPU010000303.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 4954)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4954)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4954
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_224228"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(505..933)
                     /locus_tag="VNY06_06945"
     CDS             complement(505..933)
                     /locus_tag="VNY06_06945"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HWX14573.1"
                     /translation="MSRWQAGIWVVGIVLVDHYLCGWIRRTINTALPATVLGSYPEAH
                     GWLHIVDAVFGLALVAYSEEIVFRRCARHLFQIYLNDGYAMVVVTSILFGAYHWWTGI
                     GNIIEAVLIGILLMLFYRRSAALWPVVLGHYVTDIVDFAL"
     gene            1415..1621
                     /locus_tag="VNY06_06950"
     CDS             1415..1621
                     /locus_tag="VNY06_06950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015688679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX14574.1"
                     /translation="MSKQAMREETERLVKEALERKAITIKQGKTRIESKCAKCGALNR
                     ISAAQGEVRVSYTCKECREEQRTF"
     gene            2144..2986
                     /locus_tag="VNY06_06955"
     CDS             2144..2986
                     /locus_tag="VNY06_06955"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006610280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HWX14575.1"
                     /translation="MGMLDNKVAIVTGATSGIGKRTAELFVTEGAAVVFTGRRKAEGE
                     AIAQRLGKGASYVPADATSEADWQRVTSFAMDRHGRIDALFNNAGGPAPTGGIEDISV
                     DGFDNAMALLVRSVMLGMKHVTPIMKRQRSGSIVNNGSVAAHAAGYSSSMVYSAAKAA
                     VVHFTRCVAMELGEHGIRVNSVSPGAIATGIFAKALGMTDASKADALADRMASNFATM
                     QPIPRSGMPDDIAQCVCWLVSDRSTFINGEDVIVDGGLVRGRLFTPQQTGLRQIKSML
                     GLND"
     gene            3114..3386
                     /locus_tag="VNY06_06960"
     CDS             3114..3386
                     /locus_tag="VNY06_06960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX14576.1"
                     /translation="MSDIPQCPRPFTDRLAIEFIVTRAANDGSEWICVLVEGKSSELE
                     RMTQKPLGPSPYFVMLDDCEDSPQVPIHGVASTACLKVIPRITDLL"
     gene            complement(3700..4134)
                     /locus_tag="VNY06_06965"
     CDS             complement(3700..4134)
                     /locus_tag="VNY06_06965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX14577.1"
                     /translation="MPFGKYRGKTFPEIIVRDLDWFFWVAPKLYGKLADEGEELARKA
                     RAVKIPIPRRKHLEVEYTYEDGNRFCGFAFVEADSARYSRRATRLPHLDLSWPLRRKK
                     YDKRAGRIMIRDFRIHYFGAHKRLTKERCEEFFSNDRYFIDI"
     gene            complement(4389..>4954)
                     /locus_tag="VNY06_06970"
     CDS             complement(4389..>4954)
                     /locus_tag="VNY06_06970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX14578.1"
                     /translation="RIVAEAADLLPAEARGTFLRRVVAEVREGRSFKDSDIERAVRVA
                     LFGQPMKQASVSSTDQGGERKLRSDTPTDSQEALASHPFAALLQRITGLVRLRGFSRA
                     FNFFISVNSRCGRARGLLRRHFEFGERHVREREEHIALQREIVAHLEHLREPVTLQKA
                     RDLLRNMEHAQMLHVAERNQLRELLGD"
BASE COUNT         1109 a         1416 c         1411 g         1018 t
ORIGIN      
        1 agcggcgctt acgttaatct ccggttcgct ttacgcgcaa tttggtaccc agggtttctg
       61 ggcgatgttt ctaatgccgt tggcggcgct gcccgcgatc tggcgacttt accgagcacg
      121 tagatcagcc atcacctgcg cggctgagca aatcatatag taggccatcg aaacaaaagc
      181 cggcggccct actatcgacc gaagctgtag ccttgcgggc ttgcctttcg gcattgttat
      241 ttcacactgt tgatcactta gccaccgcga gcatgcgcac gcttcctcgg tacacatatg
      301 agttggaaaa aattcgaccg tttgggggtt ttgtcgaacc acctaaaagc catggttcaa
      361 accaagcaca tcgcagccgt ctcccgtctt gttatcgacg ccgtttccag atgtgcagcg
      421 aatgacggca gtaaatgggt ctgcgttccg gtcgaatggg agattggcaa atttgaacgc
      481 ctgtcgcaga agccttccac gcactcataa cgcaaagtcg acgatatcgg tcacgtagtg
      541 tccgagcaca accggccaaa gcgctgcaga tcggcggtag aacagcatca acaaaatacc
      601 aatcaataca gcctcgataa tattcccaat gccggtccac cagtgatagg caccgaacaa
      661 tattgacgtg acgacgacca tggcatagcc gtcattcaag taaatctgga acaggtgacg
      721 cgcacaccgg cgaaacacga tctcctcgct gtaggcaaca agcgcgaggc cgaagaccgc
      781 gtcgacgata tggagccagc cgtgcgcttc agggtagctc ccgagtactg ttgccggtag
      841 cgccgtatta atggtccgcc ggatccaacc acataaatag tggtcaacga ggacgatgcc
      901 gacaacccat ataccggctt gccaacgcga cattcggagt cgcttcgccc ggaaagctac
      961 cgtccgtgcg gatggaatag cgcccaggac cgccaaggcc ccgaggcgcc cagcgtaatc
     1021 ccaaaaaatc catgttgctg catcagagcg ttccagtcgt acgatttgcg acaccaccat
     1081 tgggacaagt gccgccacga accagaccgg cgcgggaatg gcgcgaagcg acatagccga
     1141 cgtctattac ctgccaattg gtgacgaaga agagcgcgga gtgatcgtag ctggcaaatt
     1201 acccaggtat agcgtaacag aaggaatgca accgggcggc cccatcttgg gctttttatc
     1261 gacggcaatt tcgggccagc aagcggttct aggcttggtt gggccgcgga tggggttggt
     1321 tgcgggacgc gctcgtgcac gatggtgacc caatcgttgc tgtggtcctc gagtggctgt
     1381 atgacctatc gtgcagaaag caagaaagcg cgaaatgtca aaacaagcaa tgcgcgagga
     1441 gaccgaacgt ttggtgaaag aggcattgga gcgcaaagcc atcaccatta agcagggcaa
     1501 gacgcgtatc gagagcaagt gcgcaaagtg cggcgcgcta aatcgcatct ccgccgctca
     1561 aggggaggtt cgagtgtcgt acacgtgtaa ggaatgtcgc gaagaacaaa ggacattctg
     1621 aggagattcg gccgcggatt attgagccgg gagatcgtac cccggatcgg gcagtcgccg
     1681 gtctttattc gcactatgac ggccgctaac aacgcaggaa gtgcgacgct caggcagcta
     1741 ttggccaagt tcgaggcgta accaactagg cgagaaactg actccttagc tgctcgcgac
     1801 gcgctgtgag ccgctcgcaa gcggccgcaa actgcagcac aaccacaaac agagtgtcgc
     1861 ctgcaggaca gctgcatatc ggcgctggtc atggctcatg attgtgcagg ccggacagcg
     1921 tgtctaggct agccccgcat tgcccttggc gagtccgcta gtgcgttttg tagtcagccg
     1981 cccccgggcc ccccagccat tgattacgaa ctgtatcagc ggcctcggct gaatcaccgc
     2041 ggggcatatg ggctaagatg tcggtgcgaa gcgaccgaag gcacatgtcc gagaaaccct
     2101 tgccaaaggc gagagtgcaa gcgcacacaa agagggctca gagatgggga tgctcgacaa
     2161 caaagtggcg attgtgactg gcgcaaccag cgggattggc aagcgaactg ctgagttgtt
     2221 cgttaccgaa ggcgccgccg tggtctttac gggacggcgc aaagccgagg gtgaggcgat
     2281 tgcccagcgg cttggtaaag gtgccagcta cgtgccggcc gatgcaacaa gcgaagcgga
     2341 ttggcaacgc gttacaagct ttgcaatgga tcgccacggt cggattgacg cccttttcaa
     2401 caatgccgga ggcccagcgc cgacgggcgg cattgaggat atctccgttg atggatttga
     2461 caatgcaatg gcccttctcg tgcgctcggt aatgctggga atgaagcatg tcactcccat
     2521 aatgaagcgg caacgcagcg ggtcgatcgt taacaatggt tcggtcgccg cgcacgcggc
     2581 cgggtactca agttcaatgg tttacagcgc ggcgaaggcc gcggtggtac acttcactcg
     2641 ctgcgtggcg atggagcttg gcgagcatgg tatacgtgtt aactccgtat cacccggcgc
     2701 tattgccacg ggcatttttg ccaaggcact cgggatgacg gacgccagca aggctgatgc
     2761 ccttgccgat cggatggcgt ccaactttgc aaccatgcag cctataccgc ggtccggtat
     2821 gcccgacgac atcgcccaat gtgtctgctg gctggtctcc gaccgatcga ccttcatcaa
     2881 cggcgaggac gtgattgtcg acggcggctt ggttcgtggc cgtttgttca cgccgcagca
     2941 gacgggccta cgacagatca agtcgatgct tgggctcaac gactgaccct cttcgggttc
     3001 cccacctctc gtcgggcact ccgcccaaca tattttgcgt acaaaccggc gcacgcattg
     3061 atcccaccca tgttcttgac aaatgttttc agccgatttt ggggcgcctt gcaatgagcg
     3121 acataccgca atgcccgcgc ccatttactg atcgtcttgc tatcgaattc attgtcacac
     3181 gtgcagcgaa tgacggcagt gaatggatct gcgttcttgt cgaagggaag agtagcgaat
     3241 tggaacgcat gacgcagaag cctcttggtc cttcgccgta ctttgtaatg ctcgacgatt
     3301 gcgaagacag cccccaagta cccatacacg gcgtcgcgag cacggcttgc ttgaaagtca
     3361 tcccacgcat cacggacctt ctctaggctt cgcgtagctc gcgatcctgc agtcttgcct
     3421 ccggagtcgt gagctgattg ccaaagcccc cttggaaccg gcacggcctt agttatcgac
     3481 gccccgcccg agcttggcca agcacctcga caacccggtg aaatgtcgca catttccgca
     3541 gcagtgagct cggacggtgt acatgtagtc ggagtgtgcc tcggccaggc ttcttcctct
     3601 cgggccctgt aaagccactc gtaataatcg aagttgtcgc cttgccgtat cagagcagcg
     3661 acgtaattcg cagttcttgg cgacagcaaa ggaggcgccc taaatgtcaa taaagtatcg
     3721 atcattgcta aaaaactctt cgcatcgctc tttggtcaac cgcttgtgtg cgccgaaata
     3781 gtggatccgg aaatcccgga tcatgatgcg gcccgctcgc ttgtcatact tctttcggcg
     3841 aagaggccac gacaagtcga ggtgtggaag tctcgttgcc cttcgggaat atcgggcgct
     3901 atcggcttcg acgaatgcaa agccgcaaaa cctgttaccg tcttcgtatg tatattcgac
     3961 ctccagatgc ttccgacgcg gaattggaat tttgacggcc cgtgccttcc gcgcgagctc
     4021 ttccccctcg tcggcgagtt tgccgtagag cttcggcgct acccagaaga accagtccaa
     4081 atcccgcaca attatttcgg ggaaagtttt tccccgatat ttgccgaacg gcacgacgct
     4141 ccaatgcatg tcgattacct tttcgcctaa attcgtcgcg ttcgcctcag tggccgctaa
     4201 ttgcgcactt gcaaatacaa attagcgtgc tcaaaaagcc gctcgtcggc cgtgcgaggg
     4261 cagtattgcc gataatggtg accgccaccg gacgaaaacg aacaattgtg acgaatgatg
     4321 cgtcgacggc ccgggaagtg ctgggaaact aatggtcggg cggtgggtgg gccgcatcca
     4381 gggccctctc aatctccgag gagttcgcgc agttggttgc gctcggcgac gtgcagcatc
     4441 tgtgcatgtt ccatattgcg caataggtcg cgcgcctttt gaagcgtaac tggttcccgc
     4501 aggtgttcaa ggtgcgccac gatttcgcgc tgaagagcga tgtgctcctc cctctcgcgc
     4561 acatggcgct cgccgaattc aaaatgccgc ctaagcaagc cgcgggctct cccgcatcgg
     4621 ctgttaacac taatgaagaa attgaatgcc cttgaaaatc ctctgaggcg aaccaaacct
     4681 gtaatgcgtt ggagcaatgc ggcaaacgga tgagacgcta aagcctcctg agaatcagtg
     4741 ggcgtgtccg agcgcagctt ccgttcgccg ccctggtccg tcgaggagac ggacgcctgc
     4801 ttcatcggct gaccgaacag agccacgcgc actgcccgtt cgatatcgct atccttgaaa
     4861 ctccggccct cgcgcacctc ggccacgacg cgtcgcagaa acgtcccgcg tgcctctgct
     4921 ggcaacaggt cggcggcctc agcgacgatg cgaa
//
LOCUS       DATMPU010000304         4553 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_2385775,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000304 DATMPU010000000
VERSION     DATMPU010000304.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 4553)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4553)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4553
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2385775"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..159
                     /locus_tag="VNY06_06975"
     CDS             <1..159
                     /locus_tag="VNY06_06975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018455743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX14579.1"
                     /translation="DQESPSEILSDDAIVTKLKGANIDIARRTVAKYRESLKIPSSVE
                     RRRIKRKS"
     gene            615..1205
                     /gene="raiA"
                     /locus_tag="VNY06_06980"
     CDS             615..1205
                     /gene="raiA"
                     /locus_tag="VNY06_06980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003609038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HWX14580.1"
                     /translation="MTLRVSGKNMDIGDALREHIEARIDATLEKYRAGPAAGHVTIER
                     EGSGFRADCTLHLKSGATVQADSQAHEPYASFNRAADFIENQVRRHRERLLNHHVSAA
                     AEGRRGSEHLARTQAPAIRGGEAAPEQSEARELHPAVIAESSPLCKEMTVSGAAMELD
                     STNAQVIFFRHASDGRMNVVYRRPDGNIGWIDPGWE"
     gene            1300..1764
                     /locus_tag="VNY06_06985"
     CDS             1300..1764
                     /locus_tag="VNY06_06985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012385833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HWX14581.1"
                     /translation="MLLNQLITPEAVVSSLKVNTKKHALQELSDRAGSISGLPPREIF
                     DALLQRERLGSTGIGEGIAIPHGKLSQAQAIFGIFARLARPIDFHALDGVPVDLICLL
                     IAPESAGADHLKALARMARLLRDPKVTAKLRAARDVAGLYAVLTGERASHAA"
     gene            1981..2934
                     /locus_tag="VNY06_06990"
     CDS             1981..2934
                     /locus_tag="VNY06_06990"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012385837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HWX14582.1"
                     /translation="MRTYLDFEKPVAELEAKVLELRELAEKGDAVAIGDELSRLEAKA
                     EKALADLYAGLTPWQKTQVARHPQRPHFNDYIKAFVRDFTPLSGDRHFGEDAAIVGGF
                     GWFCGRAVCFLGQEKGSDTEARIKHNFGMPRPEGYRKAARIMQLADRFGLPIVSLVDT
                     AGAFPGVDAEERGQAEAIARATDASLLLGVPSVAVILGEGGSGGAIAIASCNRVLMLE
                     HAVYTVASPEASASILWRDSSRAEDAATSMKITAQDLLKFGVIDSIVTEPVGGAHRDP
                     KAAISATGDAIGSALAEFTGMSPSDIRASRTAKFLNIGRKL"
     gene            3161..>4553
                     /locus_tag="VNY06_06995"
     CDS             3161..>4553
                     /locus_tag="VNY06_06995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020174309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HWX14583.1"
                     /translation="MQFRPFAKRLLALSWLAPAGGLLSGCEESSLSPLTRSLSPIPPK
                     TVAAIEAIGSSKSAPVLIRAYKKEAEFEIWKMKADGRYGYLKSFPLCRWSGQLGPKVR
                     EGDRQVPEGFYSITPARMNPQSAYYLSFDVGYPNAYDRAQGHTGGSIMVHGACSSAGC
                     FSMTDAQIAEIYAIAREAFAGGQRAIQMQSYPFRMTAENLAKYRLDPNVGFWKQLREG
                     SDNFEVTKQYVVVGVCDKHYVFNSIPINGSQMNATGPCPAFKQDSTVHDQVAANHARD
                     ESKIAELATHGVRPVKTVYSDGGQHPNFAPLASYASRPEALAQGPVDVALDESKPNKA
                     RVAAAAKKGAAKRNTAQAGANPVPKPAEPVTVAAAGPAEPAKPESSSFFSRLWGAKPT
                     EATAAAPPDASVSVTSAAASEPATAKKVPLPPKRKEPSTSGAKPADPKAKLQNSVAAP
                     APVQPHAALSPSEA"
BASE COUNT         1007 a         1280 c         1307 g          959 t
ORIGIN      
        1 gatcaggaaa gcccgtcaga aattttatcc gacgatgcca ttgtgacaaa gctgaagggg
       61 gctaatattg acatcgcgcg gcgcacggtc gccaaatacc gcgaaagttt gaaaattcca
      121 tcctcagtgg agcggcggcg gatcaaaaga aagagttgag aaagggtcac aaatcagcgc
      181 ataggctttt agcacacaac tacggagagt gcattttggg tatttggcag ggaattcgct
      241 ctcgcacgtt tggcacggat cgcgcgaaag cgccttttaa gctcgcagga gcgacattaa
      301 aactcgcaga aacggcattt attaggttgc tatccaaccg acggggaaga gcgcttgcgg
      361 cgttgacttc aatcggagag acatttaacg tttgacgaaa cacggccata ttcactaatt
      421 cgtctcagac tctccgagga ggcaatgcgg ccacgcgtcg ctgagcaaca aaatggacaa
      481 atttcatgcg tcctagaatg ttggaaagtt gatcgttatc ggatcgtccc aacgactcga
      541 ggcttaaatc cggcatttca ggggcgttgc gccggccgaa gcggcgggcc ggaattggta
      601 acggagaatg ggcaatgacg ttgcgcgtct cgggcaagaa catggacatc ggtgatgctc
      661 tgcgcgaaca catcgaggca cgaattgatg caactttgga gaagtaccgg gctggtccgg
      721 ccgccggtca cgtgacaatt gaaagggagg gatcgggttt ccgggctgac tgcactttgc
      781 acttgaagtc gggcgccacc gtgcaggctg attctcaggc ccatgaaccc tatgcaagct
      841 tcaatcgtgc tgccgatttc attgaaaatc aggtgcggcg acatagagag cgtctgctca
      901 atcatcacgt cagcgcggcg gcggaaggac gccgcggctc agaacatctt gcgcgcaccc
      961 aagcgccagc tattcggggc ggcgaagctg cgccggagca aagcgaagcg cgggaactgc
     1021 atccggcagt gatcgccgaa tcgtcaccgc tgtgtaagga gatgacagtt tccggtgcgg
     1081 caatggagct tgattcaacc aacgcccaag tcattttctt tcgacatgct tccgacgggc
     1141 gaatgaatgt cgtatatcgc cgccctgatg gaaatatcgg ctggatagat ccaggctggg
     1201 aatagcctat cccttggttt acgcgaggcg gcctcccttg cgcgagccgg gaggatcata
     1261 ccgatcttca aacatccgca gaccggaaaa tttcaaacta tgctactgaa ccaactgatc
     1321 accccagagg cggttgtttc ctctcttaag gtgaacacaa agaagcacgc gctccaggaa
     1381 ttaagcgacc gcgcgggatc gatttccggc cttccgccgc gagaaatctt cgatgccttg
     1441 ctccagcgcg agcgtcttgg ctcgaccgga atcggggaag gcatagcgat acctcacggc
     1501 aagctttccc aggcacaagc aatctttgga atatttgcga ggctcgcgcg cccgatcgat
     1561 tttcatgccc tcgacggcgt tccggttgac ctcatttgtc tcttaattgc tccggagtcc
     1621 gccggtgctg atcacttaaa agctctcgcg cgcatggcac gtttgttgcg cgacccaaaa
     1681 gttacggcaa agcttcgtgc tgcacgtgac gtcgccgggc tttatgccgt attgaccggt
     1741 gagcgcgcct cgcacgcggc ttaggtggcg gcctttcttc aaaaatttgc cgatccgctt
     1801 tcttgacttc acccaatgaa atagcgactt tccgcgctcc tgaagcgctc ggcggcggga
     1861 gcacacacgc aagtggtgct gctagcctgt tgcggcctcc ggtttcttgc ctcgcaatgg
     1921 cagaacttct ggaacactgc catggccact ccgcgccttg gcaaaaacct acgggccgcg
     1981 atgcgaactt atctcgattt tgaaaagcca gtcgctgagc tcgaagctaa agttttggaa
     2041 ctgcgggagc tcgcggagaa aggagatgcc gttgccattg gcgacgaatt gagccggctc
     2101 gaagcgaagg ccgaaaaagc gttagctgac ctctatgctg gtcttacgcc ttggcagaaa
     2161 acacaagtcg cgaggcatcc gcaacggcca cattttaatg attatatcaa agcgttcgtg
     2221 cgggatttca cgcccttgtc aggcgacagg cactttggcg aggacgccgc aatcgttggc
     2281 ggatttggct ggttctgtgg ccgggcagtc tgcttcctcg gacaggaaaa aggttcggac
     2341 acagaggcgc gcataaagca taattttggc atgccgcggc ctgaaggcta tcgcaaggcg
     2401 gcgcggatca tgcagcttgc cgaccgattc ggcctgccga ttgtttcgct tgttgatacc
     2461 gcgggcgcgt ttccaggtgt cgacgcggaa gagcgcggcc aagcggaagc aatcgctcga
     2521 gcgaccgacg catcgctctt gcttggggta ccgagcgtcg ccgtcatact tggtgaaggc
     2581 ggctctggcg gcgctattgc gatcgcaagc tgcaaccggg tcttgatgct ggaacatgcg
     2641 gtctatacgg tcgcgtcgcc ggaagcatcg gcctcaatcc tttggcgcga ttcctcccgc
     2701 gctgaggacg cggcaacgag catgaaaatc accgcacagg atcttctcaa gttcggggtc
     2761 attgactcaa ttgtaaccga gccggttggt ggcgctcacc gcgatcccaa ggcagcaatc
     2821 tcggcaacgg gtgatgcaat cggttcggca ttggccgaat ttaccggcat gtcgccgagc
     2881 gacatccggg cgtctcgaac cgcaaagttc ctgaatattg gccgcaagct ttagtcagtc
     2941 tggctattcc ttctcggcgg cggcaaaaac ccgccctcca ggcggcctca aaattcgttt
     3001 ccttccttgt gttcgcctgc attgcaactt catttggcca tgaacgcgct tttagaatgg
     3061 tcgcggtcaa atcaacgttc cggtaacttt tgcataagga aaccgggtgt agggtggttt
     3121 gctttttcgg caattccaac cttcggcggg cgaacgatca atgcagttca ggccattcgc
     3181 aaagcggctc cttgccctct cttggttagc gcccgcaggc gggctattgt caggctgcga
     3241 ggagtcgagc ctttcgccct taacccgttc gctgtcgccc attcctccta aaacggtagc
     3301 cgccattgag gccataggct cttccaaaag cgcgccagtt ctcatccgcg cctataaaaa
     3361 agaggccgaa ttcgaaatct ggaagatgaa ggcggatggg cgctatggct atttgaagtc
     3421 cttccccttg tgccgttggt cggggcagct cggacccaag gtgcgggagg gtgaccgcca
     3481 ggtgccggag ggtttctatt cgatcacccc ggcgcggatg aacccgcaat ccgcctatta
     3541 cctttctttc gacgtaggct accccaacgc ctatgatcgt gcccagggcc atacaggcgg
     3601 ctcgatcatg gttcacggtg cctgttcttc ggcaggctgc ttctcaatga ccgatgcgca
     3661 gatcgccgag atttacgcaa tcgcaaggga agctttcgct ggcggtcagc gcgccattca
     3721 aatgcagtcc taccctttcc gcatgaccgc ggagaacctt gccaaatata ggcttgatcc
     3781 aaacgttgga ttctggaagc agctgaggga aggttccgac aattttgaag tcaccaagca
     3841 gtatgtcgtg gtcggggtct gcgacaaaca ttatgtgttc aattcgattc cgataaacgg
     3901 atcacaaatg aatgcaactg ggccctgccc agctttcaaa caagatagca ctgtgcatga
     3961 tcaagtggcg gccaatcatg cgcgcgacga atcgaaaatt gccgaacttg ccacccatgg
     4021 ggtgcgccct gtcaaaacag tttactcaga tggcggccag catccgaact ttgccccgct
     4081 ggcgagttac gcaagccgcc cggaggcgtt ggcgcaagga ccggtcgacg ttgccttgga
     4141 cgaaagcaag ccgaacaaag ccagagttgc ggctgcggcc aagaagggcg ccgccaagcg
     4201 aaatacggcg caggcaggag ccaatccagt tcccaagccg gctgagcctg tgacagtcgc
     4261 cgcagccggt cctgccgagc cagctaagcc cgagagcagt tccttcttta gccggttatg
     4321 gggtgcgaaa ccgacagagg caacggcagc agcgccgcca gatgcctcgg tctcagttac
     4381 ctctgctgcc gcctcagagc ctgcgacggc caaaaaagtg cccctgccgc caaagcgtaa
     4441 ggagccctcg acgtctgggg ctaaaccagc cgaccccaag gctaaactac agaattccgt
     4501 ggcagccccg gcacctgttc agcctcacgc tgcattgtcg ccttccgagg ctg
//
LOCUS       DATMPU010000305         3459 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_3649419,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000305 DATMPU010000000
VERSION     DATMPU010000305.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 3459)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3459)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3459
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3649419"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(482..3082)
                     /locus_tag="VNY06_07000"
     CDS             complement(482..3082)
                     /locus_tag="VNY06_07000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020173645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015616 - DNA translocase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HWX14584.1"
                     /translation="MRTTAITTALAQIPEPIRAFATRRGAEVVGLALIGAAAGIGLAL
                     ASWSVADPSFNHAASGPVHNLLGAPGAVAADLIMQLLGLSSIAFLAPPSLWGWRLITA
                     RRLERLRARLMLWLAGSLCSAGLASLFPVTARWPLPTGLGGVAGDALLMLPQRLFAGH
                     GAGMLLFAAILAGGAILCFSASVSLRVYGSTDAGDDEEDKPQLSSPPPFDGDQDEAGE
                     PGLALVLLGAFIHAGLSLKAALARSIARWRAPAAPPWPRGAVQREHAFDRPLDLAVEP
                     LNWSGSLEAPSNFDASAYAPPAARRASEPVPQGQRPSQDLDIYAPRRVIAAAAAQLKP
                     GTRALREAQPSLLDRLARQDYVFPPLHILAEPRRSAANRISEDALDQNARLLESVLED
                     FGVKGQIINVRPGPVVALYELEPAPGIKSSRVIGLADDIARSMSALSARVAVVQGRNV
                     IGIELPNQQRETVYLRELLASQDFEKSKHRLAIALGKTIGGEPVIVDLARMPHLLVAG
                     TTGSGKSVAINTMILSLLYRLKPQECRLIMVDPKMLELSVYDGIPHLLTPVVTDPKKA
                     VVALKWAVREMEQRYKNMSKLGVRNIEGFNARVAEAAAKGETLTRTVQTGYDPVTGEA
                     VYEQESMQLSVLPVIVVIVDEMADLMMVAGKDIEGAIQRLAQMARAAGIHLIMATQRP
                     SVDVITGTIKANFPTRISFQVTSKIDSRTILGEQGAEQLLGQGDMLYMAGGGRISRVH
                     GPFVTDSEVEKIVAHLKTQGQPEYLDSITEDDVLDDEDEAGPAPGSMDAEEAGDLYDR
                     AVAIVLRDKKCSTSYIQRRLSIGYNKAASLVERMEDEGIVGAPNHSGKRAILVAGGID
                     RGAFDVASEG"
BASE COUNT          732 a         1051 c          991 g          685 t
ORIGIN      
        1 gagggtaatt ccgtaagcca taacttacag taagcaaaaa cttacctaca tgtcaagata
       61 ttacatctgg gtttttgccg acgtgggacg aggttttcgt ggtgcaaaat tccggtgcga
      121 aaatgcgccg tctttatagc cgacgtatcg tcagataccg gtgccggacg agaccaagca
      181 gcggttcggt cggttcaaat gccagcctcg aaatgagtga atcttacatc ggagctgact
      241 tccggcaaca tgcttcatca tcgaggcacc gccgccgccc aatttttttt gcaccaaaaa
      301 atcaaccgat cttacaaatc gaacgagtaa ccggtgtttt cggcttgtaa ggttttgggc
      361 cgcaatcgga caaaaatgtt tcacgtgaaa catttttgta aggttttgtt tgccagcaac
      421 cagccccggc ttgtgggttt cataagccgc ccgtctcgcg cgcggtgatt gctcctctat
      481 atcacccttc gcttgcaaca tcgaacgcgc cccggtcaat accgccagca acaagaatcg
      541 cccgcttgcc ggaatgattg ggcgcaccaa caatcccttc gtcctccatc cgctccacaa
      601 gggaagccgc cttattgtag ccgatggaga ggcggcgctg gatatagctt gtcgagcatt
      661 ttttgtcgcg caaaacgata gcgacagccc ggtcgtaaag gtcgcctgct tcctcggcgt
      721 ccatgctgcc cggcgcgggc cctgcctcat cctcgtcgtc caaaacgtca tcctcggtga
      781 tggagtcaag atattccggc tggccttgag tcttcaaatg cgcgacgatc ttttcgactt
      841 cactgtcggt gacgaatggc ccatggacac gcgaaatgcg cccgccgccc gccatgtaaa
      901 gcatatcgcc ttggcccagt agctgctcgg cgccttgctc gccaagaatc gtgcggctgt
      961 cgatttttga ggtaacctga aacgaaattc tggtcgggaa attcgccttg atcgttccgg
     1021 taatgacatc gacggaggga cgctgcgttg ccatgatgag atgaatgccc gcagcgcgcg
     1081 ccatctgggc aagccgctga atcgcgccct cgatgtcctt gcccgcaacc atcatcagat
     1141 cggccatctc gtccacgatc acaacgatga caggcagaac cgaaagctgc atcgattctt
     1201 gttcgtagac cgcttcgcca gttaccggat catagcctgt ctggaccgta cgggtgagcg
     1261 tctcgccctt ggcggctgcc tccgcgaccc gggcgttaaa cccttcaata ttgcggacgc
     1321 cgagcttcga catatttttg tagcgctgtt ccatttcgcg caccgcccat ttgagcgcga
     1381 caacggcttt ctttggatcg gtaacgacgg gcgtcagcag atgcgggatc ccatcataga
     1441 ccgagagttc gagcatcttg ggatcgacca taatgagccg gcattcctgc ggcttcagcc
     1501 ggtagagcaa agataggatc atcgtattga tcgcgaccga cttgccggat ccagtcgtgc
     1561 ctgcgacgag gagatgcggc atgcgcgcaa gatcgacaat gaccggctcg ccgccgattg
     1621 tcttgccaag cgcgatcgcc agacgatgct tggatttttc gaaatcttgg ctagcaagca
     1681 attcgcgcag ataaacggtc tcgcgctgct ggttcggcaa ttcgatgccg ataacatttc
     1741 gcccctgcac gacggcaaca cgagccgaga gggccgacat cgagcgcgcg atatcatcgg
     1801 caaggccgat gacccgcgac gatttgatgc cgggggcggg ctcaagctca taaagcgcta
     1861 ccaccgggcc tggccgcacg ttgatgatct ggcccttgac gccaaaatcc tcaagcacgc
     1921 tttcaagcag ccgcgcattc tggtcgagcg cgtcctcaga gatcctgttt gcggccgatc
     1981 tccttggctc cgctagaata tggagtggag gaaaaacata atcttgccgc gccagacggt
     2041 cgagcagcga cggctgcgcc tctctcagcg cccttgtccc cggcttcaat tgcgccgcgg
     2101 ccgcagcaat cacccggcgc ggcgcatata tatcgaggtc ttggcttggg cgttgtcctt
     2161 gcggcaccgg ctcgcttgcg cggcgagcgg caggcggagc ataggcgctc gcatcgaaat
     2221 ttgatggcgc ttcgagagat cccgaccaat tcagcggctc gacggcgaga tcgagcggcc
     2281 ggtcaaaggc atgttctcgc tgaaccgcgc ctcggggcca tggcggcgcg gccggggccc
     2341 gccaccgcgc aatggaacgc gccaatgctg ccttgaggct gaggccggca tgaatgaagg
     2401 cgccgagcaa aaccagcgcg aggcctggct cacccgcttc atcctgatcg ccgtcaaacg
     2461 gcggagggct ggacaattgc ggcttgtcct cctcgtcgtc gccggcatcc gttgaaccgt
     2521 aaacccgcag agagaccgaa gccgagaagc aaaggatggc accccccgcg agaatcgcgg
     2581 caaaaaggag cattccggca ccatgacccg cgaaaagccg ctgcgggagc atgagaaggg
     2641 catcccccgc gaccccgccg aggccggtcg gtagcggcca acgcgccgtg accggaaata
     2701 gcgaagcaag ccctgccgaa cacaggctgc ccgccagcca gagcatcagc cgggccctca
     2761 aacgttcgag gcgccgcgcg gtgatcagcc gccatcccca taggctgggc ggcgctagga
     2821 aggcgatgga agagaggcca agcaattgca tgatgagatc ggctgcaacg gcccctggtg
     2881 caccgagcaa attgtgcacc ggccccgacg ccgcgtgatt gaagctcgga tcggcgaccg
     2941 accaggaagc cagcgccaag ccaattcccg ccgctgcgcc aatcagggcg aggcccacaa
     3001 cctcagcgcc cctccgcgtc gcgaaggccc gtatcggttc tggaatttgg gcaagcgccg
     3061 tggtgattgc tgtcgtccgc atgcgatcct gccgccttcg cgccactgcc aatttgcttg
     3121 atccttccgc tggccacttt ggcgagaaaa gcaggaccca agaaaccttt ccggctctgg
     3181 cgtgtgaaag tcacctgaaa acaatcgtcc tagatcagga tgatcaggat cctctttgct
     3241 ttctcgctgg gagtttgcca aagaagcggc gccggcaaac cttgcggcaa attaacggga
     3301 ggcggttaaa gctggcttaa ccttgcgcgc ggggaattgc ttccgataga cgaacgcctt
     3361 gtgcccgcgg actgactagt acccactttc gctggtgcgt gagtcatgat tcatgggtgc
     3421 atgaaaagca tccctgcggc gacgccaatc acattgacg
//
LOCUS       DATMPU010000306         2699 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_3956807,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000306 DATMPU010000000
VERSION     DATMPU010000306.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 2699)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2699)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2699
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3956807"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            397..1581
                     /locus_tag="VNY06_07005"
     CDS             397..1581
                     /locus_tag="VNY06_07005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012171610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HWX14585.1"
                     /translation="MGLNPKPFDVRELVSSVERAMSEPKNRSRTKPAADVERTPLVGR
                     SSAMQEICRALERLMQTDLTVMITGESGTGKELVARALHDYGKRKRGPFVAINMTAMP
                     RDLIESELCGHEKEAFTGAAQRYAGCFEQAEGGTLFLDEIGDMPMEAQTRLLRVLQEG
                     EYRTVGSRNPIKTNVRIIAATNKDLGGLIRQGLFREDLFFRLNVVPLRLPPLRERLDD
                     IADLAQHFFALAAAEGLPFKRIEVDAVERLKRHRWPGNIRELENLTRRLAALYPQETV
                     TPQLIELELGVETSAVLQDYSAAHNPQDVRKGKPGRSETLATAMKCHLSEVFHNHGES
                     LPPPGLYYRILREIEYPLICAALAATRGNQIKAAELLGLNRNTLRKKVRYLDIKWMRS
                     AS"
BASE COUNT          582 a          749 c          791 g          577 t
ORIGIN      
        1 cggatggatg agaagacgct gatgatcggc gacgaagcag tccacggcga ggaagcctac
       61 gcaatggatt aactgtggcc ggcatcgctc acttgcgtgc tcgttcagtt tcctgccgcg
      121 tgggcttgta gcggccccaa ctaccctgct gcctttagag gtcgtggtgg agggcagtat
      181 ccgcgagtgc ccggacagtt actttgaggg cggcaaccac gcgatggtgc atgttagtca
      241 ggcctagatt gcaaggctga cggcgaatgt accgcacagg cacttcggca accatgcctg
      301 cgagatagcg gactggaagt ccagtctgtc gcgccagcgt ggggtagggc cgctaccgtt
      361 gcgcccggga atggagaccg gcgtttggtc gataccatgg ggctaaatcc gaagccgttc
      421 gacgtgcggg aactcgtttc cagtgtcgag cgagccatga gcgagccaaa gaatcgcagc
      481 cgcacaaagc cagccgcgga cgtcgagcga acgcctctcg tggggcgctc ctccgcgatg
      541 caggaaatct gccgtgcctt ggagcgcctg atgcagaccg atctcacggt catgatcacc
      601 ggcgaatccg gcaccggcaa agaacttgtc gcgcgtgcgc tacatgacta cggcaaacgg
      661 aagagaggcc cgtttgtcgc catcaacatg accgcaatgc cccgcgatct catcgagagc
      721 gaattgtgcg gccacgaaaa ggaggctttt accggcgccg cccagcgtta cgccgggtgt
      781 ttcgagcagg ccgagggagg cactctcttc cttgatgaga tcggtgacat gccgatggag
      841 gcgcagacac ggcttttgcg cgtcctgcaa gaaggcgaat ataggactgt cggtagccgc
      901 aacccgatca agacgaatgt ccgtatcatc gcggcgacca acaaggatct cggtgggctg
      961 atccgccaag gcctcttccg cgaggatttg tttttccggt tgaatgtcgt ccctttgcgg
     1021 ctgccgccat tacgcgaacg cctcgatgac attgctgacc tagcgcaaca tttttttgcg
     1081 ctcgccgcgg ccgaaggctt gccgttcaaa cgtatcgaag ttgatgccgt cgagcgcctc
     1141 aaacgacatc gctggccagg caatatcaga gaactcgaaa atctcacccg ccggcttgct
     1201 gcgctttacc cccaggaaac cgtcacacct cagttaattg agctagagct cggcgtggaa
     1261 acgtcagcgg ttctgcagga ttattcggcg gcgcacaatc cgcaggacgt gcggaaaggg
     1321 aagcctggtc ggagcgagac gctcgcaacc gcaatgaaat gccatctctc cgaggtgttc
     1381 cacaaccacg gcgaaagcct cccgcctccg ggtctctatt ataggatatt gcgcgagatc
     1441 gaatatccgt tgatttgtgc tgcgcttgcc gccacgaggg gcaatcagat caaggctgcc
     1501 gagcttttgg ggttgaaccg gaatacattg cgcaagaagg tgcgctatct cgacatcaag
     1561 tggatgcgtt cggcgagctg acgcggccac gtctcatcag ggcattggca gaaattcaaa
     1621 ctgagacact agtctagtct agactaatcg ctgtttgctt cggcggcggc gctttgttaa
     1681 agtaaagagc cttccttcaa aaccctgcgc aagaccatca tccttcgaga tatgcccttc
     1741 gggcgctccg cacgagaaaa gcgtagtgcg cagcgcgagc ttgttcttac gcggacaagc
     1801 gcgagaagct tccggccgat cgtaccaaaa tgtttcacgt gaaacatttt tgtccgaatg
     1861 gagcccaaaa cctcacaaga ccggagacag cggcccctct tcgttcgata agatcaacca
     1921 atcttttggt gcaatcggaa tcgggcggcg gcggcgcctc gatgacttga gccgttgccg
     1981 gaaaattttt ccgatgtaag gttggcctgg caccccatgc gtacctcttc agacagaccc
     2041 gatcaggctc ctgcgtcccg cggctcggct ttataatgaa gaggcgcgcg acactgaaat
     2101 ttcctgttcc cgtctatggc tgagatttct ctaacggcta tgcttgtggt tatcgggcgg
     2161 ttccactttg gcgctgccgt ttttctccgg cggtccatat agtggcggag gcctgatcac
     2221 tccgaggccg aggccaatta gaagaaagag gagcgcggcg gcggcgagga cgcctaatat
     2281 gatgccgaat ggatccatat cgtgacctcc ctgtttcgac ttgtgaagga cgaacactta
     2341 gcataacaaa tctggagcgc gcttttgttc cgaaatttcc cgcgacgtgc cagcccggga
     2401 cgtttctcaa acgccggaag gggcaatcgg gtagaggggt ttggttctac tattcccatt
     2461 ttcctttgca tcgtccgccg gataccgctg cgcttatgct tccatccgag cttcggccgg
     2521 cgcaatgctg gcccgcttgg catcaggcgc gtagggccga agccatttta atggtgactc
     2581 ggaatggtat ggggccgggt cctgctgatg agacgaaaag ggatacaagc gatgaatttc
     2641 ggtatgatat gataatgtca attcgagacg cgcttaactt tcaaaagtat cccattatg
//
LOCUS       DATMPU010000307         5828 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_1782477,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000307 DATMPU010000000
VERSION     DATMPU010000307.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 5828)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5828)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5828
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1782477"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2792)
                     /gene="addB"
                     /locus_tag="VNY06_07010"
     CDS             complement(<1..2792)
                     /gene="addB"
                     /locus_tag="VNY06_07010"
                     /inference="COORDINATES: protein motif:HMM:TIGR02786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000724 - double-strand break repair via
                     homologous recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="double-strand break repair protein AddB"
                     /protein_id="HWX14586.1"
                     /translation="MEKSVFTIAPGAPFLKTFAAALLEGRIVEGYSARLGPLELSDAT
                     IYVPTRRAARALAGELARAIGRPTMLLPRILPLGALEEMETALLMDEARPESDYDPAV
                     PEAAGEIARRMGLAELTLAWARALSHAIVSVDENGRYECDKRESFLVATSAADAWNLS
                     EELARLIDELIIEDVAWDRLDPRALPELLPEFDSYWRITLDFLTIAIKEWPKILAARG
                     LVDKARRQVALIDAQCRRLEEGMFTGPVVAIGSTGTNRATARLLAAIARAPNGAIVLP
                     GLDLDLDARSWSMIAGDSKHNIEASFTHPQSALSRLLGILKVRRDEVLSLGAVAPDLA
                     MRGKFVSEALRPAEATDGWTFYREAVDAREIGVALERVCLIEAADEREEALALAIAMR
                     HALETPCETAALITPDRKLARRVRADLQRWGVDAEDSAGEPLSARPIGALARLLIDCA
                     ISKMAAPDLAALLAHPLFRLGLSRDDIAHRSALIEIGLLRSSCAMGGLAARIFDDCSA
                     LIAAVKGQSNGPHAHPAKKPISGEEWGRIEELMARLGTLFRPLMATNGNQGLDQWVAA
                     HRAVLAKATECADDDINSEDKETLDALFDDLAHNAFEKFKLDIDSYGHFFARIAREIT
                     LREVRPAQSRLQILGLLEARLIDADVMLLGGLDETVWPPQPRTDAFLNRPMRAALGLT
                     PPERKLGQMAHDFAQAMGKRKTILSRSRKRDGTPIVASRFLQRMAALGGQAWDACRAR
                     GDFYLRLAREIDRPPPNLLPSERPLPKPPVALRPTRLSVTQIETLRRDPYAIYAEKIL
                     QLKELDPLGGPVGAAEIGSAIHLALARFVQNYPAGPLPRDVNEKLTTLLRQALAAQLR
                     DAAFAALQWPRLQKMIAFYLGFEARRRDKIAAIKTEHNGALDILLADGSVFGLTARAD
                     RIELNADGSVTLVDYKT"
     gene            complement(3077..3676)
                     /locus_tag="VNY06_07015"
     CDS             complement(3077..3676)
                     /locus_tag="VNY06_07015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018387436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbE family thiol:disulfide interchange protein"
                     /protein_id="HWX14587.1"
                     /translation="MAVTAPRDAASDRSRRALLIFAPLVVFASLAALFFVRLYSGDVS
                     ILPSALIGKDIPVFDLPPVEGLGGKPGFSDADLHQGKVTLVNVFASWCVPCHQEHELL
                     MRLASDPELARLGVRLFGLSYKDDVANVARFLTENGDPFAKVGTDRKGRTAIDWGVYG
                     VPETFVVRGDGKIAYRFVGPVTEERFRDAILPAIKKVVK"
     gene            complement(3678..3893)
                     /locus_tag="VNY06_07020"
     CDS             complement(3678..3893)
                     /locus_tag="VNY06_07020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX14588.1"
                     /translation="MSHDPHFGSVIAAYALAIVVVGGMIGAVVVDYMRLKRALSLLAM
                     PERHQQAMAESSTQDWKPQASDPEGLE"
     gene            complement(3883..4665)
                     /locus_tag="VNY06_07025"
     CDS             complement(3883..4665)
                     /locus_tag="VNY06_07025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008196280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter permease"
                     /protein_id="HWX14589.1"
                     /translation="MRFMLNYASPSHFLRLSRILIPPSALVTALLFGVGLYLAWFMAP
                     PDYQQGETVRIMYLHVPAAWLAMLIYAVMTSAALGTLVWRHPLADAAQKAAAMLGSGF
                     TLLCLGTGSIWGKPMWGTWWVWDARLTSVLVLFLIYLGLVALWRTLEDPGTAARAAAI
                     FTLVGAVNIPIIKFSVDWWNTLHQPASVFRLGGPAIAPSMLWPLIVMALAFTMLFVTL
                     YLMAIRNEILRRRLHRLLLLAAAAQEPGPAAQSSAWEAAYEP"
BASE COUNT         1337 a         1649 c         1712 g         1130 t
ORIGIN      
        1 gtcttgtagt cgacgagcgt cacggaacca tccgcattga gctcgatccg gtcggcgcgc
       61 gccgtcaagc caaataccga gccgtcggcg agaagtatat cgagcgcgcc gttgtgttcc
      121 gttttgattg ccgcaatttt atcgcggcgc cgcgcttcaa agccaagata aaaggcgatc
      181 attttttgca gccgcggcca ttgcagcgcc gcgaaagccg cgtctcgcaa ttgtgccgca
      241 agagcttggc gcagcaaggt agtaagcttt tcgttcacgt ctcgcggcaa gggaccggct
      301 gggtagttct ggacaaaacg tgcaagagct aagtggatcg cgctgcctat ctccgccgct
      361 ccaacgggtc cgccaagagg gtcgagttct ttgagctgta gtatcttttc ggcgtaaatg
      421 gcatagggat cccgccgcag cgtttctatc tgagttacgc tgaggcgggt cgggcgcagc
      481 gctacgggcg gttttggaag aggcctctcg ctcggcaaaa gattcggagg cgggcggtca
      541 atttcccgcg ccaggcgcag ataaaaatct ccgcgcgcgc ggcacgcgtc ccacgcttgg
      601 ccgccgagcg ccgccatccg ctgaaggaag cgcgacgcaa cgattggcgt gccatcgcgc
      661 ttgcgggagc ggctcaggat cgttttgcgc ttgcccatgg cttgcgcgaa atcatgcgcc
      721 atctggccga gttttcgctc tggcggcgtg aggccgagag cggcgcgcat tggccggttg
      781 aggaaagcgt cggtgcgtgg ctggggaggc caaacggtct cgtcgaggcc gccaagcagc
      841 atcacatcgg catcgatcag gcgtgcctcg agaaggccga ggatttggag gcgggattgt
      901 gcgggccgta cttcgcgcaa agtgatttcg cgcgcgattc tggcaaagaa atgcccatag
      961 gaatcgatgt cgagcttgaa tttttcgaaa gcgttatggg cgagatcatc gaagagggca
     1021 tcgagcgtct ccttgtcctc actgttgatg tcatcatctg cgcattctgt ggctttcgca
     1081 agcacagcac gatgagcagc cacccactgg tccaggccct ggttgccatt tgtggccatg
     1141 agcggcctga acagggtgcc aagccgggcc atcagctctt caattctgcc ccattcttcg
     1201 cctgaaatag gcttcttggc gggatgtgcg tgggggccat tcgactggcc tttcacggcg
     1261 gcgatcaaag cggaacaatc gtcaaagatg cgcgcggcaa ggcctcccat ggcgcaagat
     1321 gaacgcaaaa gaccaatttc gatgagcgca ctgcggtgcg cgatatcgtc gcgggaaagg
     1381 cctagcctga acaaaggatg ggcaagcagg gcggccagat cgggcgctgc cattttactt
     1441 atggcgcagt cgatcaggag cctcgccagc gcgccaatag gccgcgcgct gagaggttca
     1501 ccggcggagt cttcggcatc aactccccag cgttgcaagt ccgcgcgcac cctgcgcgca
     1561 agcttgcgat cgggagtaat tagagccgcc gtctcgcacg gagtctccag tgcgtgccgc
     1621 atggcgattg cgagcgcaag tgcctcctcg cgttcgtcgg ctgcctcgat aaggcatacc
     1681 ctttccagag cgacgccaat ttcgcgggca tctacggcct cgcgatagaa agtccatcca
     1741 tctgttgcct ctgcgggcct caaagcttcg gaaacaaatt tgccccgcat ggcaaggtcg
     1801 ggcgcgaccg cgcccaggct caaaacctcg tcacgtctga cttttagaat tccgagaagg
     1861 cgcgaaagcg ccgattgggg atgggtgaaa gaggcttcga tgttgtgttt gctgtctcct
     1921 gcgatcatcg accaggaccg ggcgtcgaga tcgagatcga gacctgggag aacaattgcg
     1981 ccgtttggcg cccgcgcaat cgccgcaagg aggcgcgcgg tcgcgcgatt ggtacctgtc
     2041 gagccaatcg cgacaactgg gccggtgaac ataccttctt ccaagcggcg gcattgcgca
     2101 tcgatgagcg ccacttggcg ccgcgcttta tctaccaagc cgcgcgcggc gagaatcttc
     2161 ggccattcct tgattgcaat ggtgaggaaa tcaagtgtga tccgccaata agagtcaaat
     2221 tcggggagaa gttcaggaag agcgcgagga tcgagccggt cccaggcaac atcctcgatg
     2281 atgagctcgt cgataaggcg agcgagctct tcagatagat tccaggcgtc ggcagccgat
     2341 gtcgcgacca gaaacgactc gcgcttatcg cattcatatc tgccgttttc atcgaccgaa
     2401 acaatggcgt gacttaaggc gcgcgcccat gcgagcgtca gttctgcaag ccccatccgc
     2461 cgcgcaattt ctcctgccgc ttcgggaacc gccggatcgt aatcactctc cggccgcgcc
     2521 tcatccatga ggagggccgt ttccatctct tccagcgcgc cgagcggcaa aatccgcggc
     2581 agcaacatcg tagggcggcc tatcgcgcgg gcaagctcgc cggccagagc acgcgccgcg
     2641 cgccgcgtcg gaacgtagat tgttgcatcc gagagttcga gcggaccgag gcgcgccgaa
     2701 tagccttcga cgatgcggcc ctcaagaagc gcggctgcga aggttttgag aaaaggcgcg
     2761 ccaggcgcga tggtgaaaac gcttttttcc aaacgagctt ttatttgtgc aaactgacta
     2821 atgctatcat gcggcttgtt ccccggcata caagcatgtt ttgcagagtt ttcccagcgt
     2881 tcgggcgttc acagtgcaat cgcaggcatt atttgcttgg cgggcgggcc tgagagacaa
     2941 cgcgcagctt caggtccttt taccggcgga gcggcgtggt cactaaccgc tgctgacggg
     3001 aacgataaga caaggcgcaa gccactttga agcaacaata gtcatcaagc gacatcacga
     3061 agagataact gtggaattat tttaccactt ttttgatcgc aggcaaaatg gcgtcccgaa
     3121 atctctcttc ggtcactggc ccgacaaagc gataggcgat ctttccatcg cctctaacaa
     3181 cgaaggtttc ggggacgcca tagacgcccc aatcgatcgc ggtacgccct tttcggtcgg
     3241 tgcctacctt tgcaaaagga tcgccattct cggtaagaaa acgcgcgaca ttcgcgacat
     3301 catccttata ggaaagcccg aacaaacgca cccccaagcg tgcaagttcg ggatcggacg
     3361 caagccgcat aagcaattca tgttcttggt ggcaaggcac gcaccaggat gcaaagacat
     3421 tgacaagggt caccttgccc tggtggaggt ccgcatccga aaatccgggc ttgccgccga
     3481 gcccctcgac ggggggcaag tcaaataccg gtatgtcctt gccaatgagc gctgaaggca
     3541 gaattgatac atcgccggaa tagagccgga cgaaaaacag tgcggccaag gaggcaaaca
     3601 caaccaaggg cgcaaaaatg agaagcgctc ggcgcgagcg atcggacgcc gcgtcccgcg
     3661 gagcggttac ggccatttca ttccaaacct tctggatctg acgcttgtgg cttccaatct
     3721 tgggtgctcg attctgccat cgcctgttgg tgccgctcgg gcatggcaag caaagagagc
     3781 gcgcgcttta accgcatata gtcaaccaca accgccccaa tcattcctcc tacgacgaca
     3841 atggcgaggg cgtatgccgc gatgacggag ccaaaatgcg gatcatggct cataggccgc
     3901 ctcccaggcg gatgattgcg ccgctggccc cggctcttgc gccgccgccg cgaggagcaa
     3961 aaggcgatgc aggcgcctgc gcaagatttc attacggatc gccatcagat agagtgtgac
     4021 gaacagcatg gtgaaggcaa gcgccatcac gataagcggc caaagcatgg atggcgcgat
     4081 cgcgggaccg ccgagacgaa agaccgaggc cggctgatgc aatgtattcc accagtcaac
     4141 cgaaaatttg atgatgggaa tattgaccgc gccaaccagg gtgaaaatcg ccgccgcgcg
     4201 tgcggccgta cccgggtcct ctaacgtgcg ccacagggcc acgagtccaa gatagatcag
     4261 gaaaagtaca aggacggagg taagccgcgc gtcccaaacc caccaagtgc cccacattgg
     4321 ttttccccag atcgagccgg tgccgaggca gagcaaagta aagcctgagc caagcatcgc
     4381 cgccgccttt tgcgccgcgt cggccagagg gtggcgccaa actagcgtgc caagtgcggc
     4441 cgaagtcatg accgcataga tcaacattgc aagccatgcc gcgggaacat ggagatacat
     4501 gatcctgact gtctcgcctt gctgataatc aggcggggcc ataaaccatg cgagataaag
     4561 gccaacacca aagagcagcg ccgtcaccag cgccgacggc gggatcagaa tgcgggaaag
     4621 gcgaagaaaa tgagaaggac tggcgtaatt aagcatgaat ctcatctagg cccgcacaaa
     4681 cgcaaatgca aacgtgtcga aacatttccg gcgttccagc cgatcagcca acggtgcgac
     4741 gtcgggggga ttaacagtcg acccgaaaat tcttggcggg ccctgcttgc ataagatcaa
     4801 cgcaaaccta ttttggtaag gaccggcgcg accagcggct aaaactgggc tttttccttt
     4861 ggcagagaat tgggcaagaa aacgatcgct tcgcccgcaa tttgcgatgg atcaatgcaa
     4921 caataggaat ccgtaacaaa aataggcatt gttcgggcat tgaaacaaat tgaaaggcgt
     4981 tacccatgct gttcccaatc attgtcgacg cggttctgct gctcgccatt ctcggctatc
     5041 ttgtctttgc tctaacccct tccgagccga tgtaaaaatt cgtgtggcgg ggcaaaagcc
     5101 accgccgctt tgagcggcgt cctttgcctt acagcggcgg gaccacgcgg tagtagagcc
     5161 cgttattgcc tagaagggct gaaaccaact ctatgccgct tcacaagccg catgtatcta
     5221 attgaaatcg ttagatttgc ctatccaaaa ctctactgtt gactatgtcg gttcatcaca
     5281 atatattgga cggatattag acaatggatt tttggacatg ccgaagaaaa ttagagaacg
     5341 atcgattgat aacaaaacgg cgcgtgcgaa acttgcgcct tccgagaaac cttactggcg
     5401 tagcgtcgac ataaggtttg caccttggct atcggaaggg cttgcacggt ggccgctggg
     5461 ttctgaggcg ctatttaggc ggccaaacat acgccaccga aagcatcggc acggccgacg
     5521 acaatcagga cgcggacaac gtttcgattc tgactttttt ccaagcgcaa gccaaagcgc
     5581 gtgagcttgc caaaggatat cgcgaggcac cggcgggcgc ggcgaagctc acagtgaggg
     5641 acgcgattga atcttacttg ggcagcagtc gggggcgcga tcagcccatt cgtctttcta
     5701 agcacgtgct aacagacgaa attgccaatc agctagttgc aagcctgacc gaatctgcgt
     5761 tgtcgaactg gcgcaaccgc ctgccggaga aactgagcgc agcgacacgg aagcgaatca
     5821 caacggac
//
LOCUS       DATMPU010000308         7350 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_870083,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000308 DATMPU010000000
VERSION     DATMPU010000308.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 7350)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7350)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7350
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_870083"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..346)
                     /locus_tag="VNY06_07030"
     CDS             complement(<1..346)
                     /locus_tag="VNY06_07030"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015666912.1"
                     /note="Catalyzes the first step in hexosamine metabolism,
                     converting fructose-6P into glucosamine-6P using glutamine
                     as a nitrogen source; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate
                     aminotransferase"
                     /protein_id="HWX14590.1"
                     /translation="MCGIVAILGKGPVAGSILEALKRLEYRGYDSAGIATLEMGRMTR
                     RRVSGKLKNLELRLAAEPLNGLIGIGHTRWATHGKPDESNAHPHVSASVGIVHNGIIE
                     NFRELRDELREKG"
     gene            complement(346..1785)
                     /gene="glmU"
                     /locus_tag="VNY06_07035"
     CDS             complement(346..1785)
                     /gene="glmU"
                     /locus_tag="VNY06_07035"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012116389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HWX14591.1"
                     /translation="MGANAFDSRDGSSSRTCLAVVLAAGDSKRMCSTRPKVLHELAGR
                     TMLAHVLSALREAGAGQVAVVVGPNHEAVIAEAKVSLRDAEIAVQKERLGTAHAVLAA
                     REVIARGYDDLLIVFADTPLVRPDTFITMRKKLATGEKAVVALGFKARQPAGYGRLIL
                     DDGELKAIREDRDATEEEREIEICNAGLMALDGRRALALLEAIDNANNQKEYYLTDVV
                     AVARSRGLAVDAEIADEYEVIGVNDRAQLAAAESILQARLRTAAMRRGATLIDPGSVT
                     LAFDTMLGSDAVIEPHVVFGPGVRVGEGTRIRSFSYLEGTAIGANAFIGPFARLRPGT
                     ALEQDVHIGNFVEVKGSDVSQGAKINHLSYIGDASIGARTNVGAGTITCNYDGVAKYR
                     TEVGENVFIGSNSALVAPIKIGAGAYIGSGSVITQDVAPDAMALGRARQVEKPGRGKQ
                     YRQARKKEPGSIMNGPENPLASSNKKGGV"
     gene            2569..4194
                     /locus_tag="VNY06_07040"
     CDS             2569..4194
                     /locus_tag="VNY06_07040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020175656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucan biosynthesis protein D"
                     /protein_id="HWX14592.1"
                     /translation="MKRKDFFSAAQIDAGKDGSMVKRREVLKFALGGIAGGITALSRI
                     CLDEAIAMPSIAEEALRTFPLSEAVPFDPNMVPPAARTLSKQAFKPLPDDLPQVLRGI
                     SYDQYAAIRPRPETAIWATDNSGFAVEPLHRGFIYSMPMEINLVEDGKARRVIYDAVL
                     FGTGTVEVPKNSGDLGFSGFRVLSRRPEGSFEVAIFQGASFFRAVAPGQTLGTMARAM
                     SIKTADLRGEEFPAIRTVWIERPTLTANALVIHALIDSESLTGAYRFTLRPREATIID
                     TECTLFARVPVDSLGLATMSATHISGSIDERRNNDLRPSVSEVGGLQMLTGKGEWLWR
                     PVANRDTLQVSTFVDDNPRGFGFLQRDRNFDHYQDDEQRFEARPSLWIEPIGDWSAGG
                     VQLVEIPSDSEANDNIIAFWKPKQPLSAGSETFFAYRQFWCWNPPEQPPLAVCIQSRS
                     GRGLSPKRRRFIVEFAGDILSNPQNAAAMKPNLTLSQGSITLLRTFTSANQRTCRLLF
                     ELDFPNQDYAEMRLVLEAAGTPISETWLYRWTP"
     gene            4182..6407
                     /gene="mdoH"
                     /locus_tag="VNY06_07045"
     CDS             4182..6407
                     /gene="mdoH"
                     /locus_tag="VNY06_07045"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004448157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis glucosyltransferase MdoH"
                     /protein_id="HWX14593.1"
                     /translation="MDAVTSPQPNETAVMELRLAESARTGDSYADTPPEAPLAMPVQS
                     LSQFDCTGRHEDIASHSLIGCWLTRLLVFGGGLALTVYGGHEMYRVVEVGGVTALEWA
                     LLFLFVANFSWISLACTSSLAGFLWLALVAPKQPKPPDALRHRTAVVMPIYNESPARV
                     FGAVAAMIEEVEASGLGRAFDWYFLSDTTDPEIFVAEEQLFLAVREKLASGRRLYYRH
                     RLKNINRKSGNIEDFVTRWGGRYEHMVVLDADSLMAGQTIVALAVAMEADPDSGIIQT
                     LPLIVNRNTLFARLQQFAARIYGPVIAAGVSITMGRDGNYWGHNAILRTKAFAAHCGL
                     PVLRGRPPLGGHILSHDFVEAALMRRAGYAVYMLPNLGGSYEECPPSLIDLSTRDRRW
                     CQGNLQHSRILPAKGLHPMSRQHLMTGIMAYAASPLWMAQLIVGIVIVVQASYIRPEY
                     FPSDFTLFPAWPRFDPQRSLELFALTMTILLLPKIFGLILALIRGVTRRGAGGAFRLV
                     VSAIFEVIMSALLAPIMMLIQTGHAMHFVFGFDTGWDPQRRDDGSIPFKAIVARHLSH
                     VAMGFLTLIAGLLISPSLVGWMSPTILGLILAILLSWGTGLRSVGLALRHAKLLMTPE
                     EHQAPAVVARANQLAEDFADLAAGHLKGLYALHGDSQYRAFHLACLPRRPKRWNGNMS
                     AEWALADAKISDAETIDEAISWLHPKERMAILQDPALIARFASLPSLPPCSDPVAASA
                     G"
     gene            7178..>7350
                     /locus_tag="VNY06_07050"
     CDS             7178..>7350
                     /locus_tag="VNY06_07050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007596152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HWX14594.1"
                     /translation="MREEVKKDTIAKAYARWAPVYDLVFGPVFERARRAAIAAAERAG
                     GRILEVGVGTGLSL"
BASE COUNT         1485 a         2242 c         2022 g         1601 t
ORIGIN      
        1 agcccttctc acgcagctcg tctcgcagtt cccggaaatt ctcgataatc ccgttgtgaa
       61 cgatgcccac acttgcgctc acatgaggat gcgcgttact ttcatctggt tttccatgcg
      121 ttgcccagcg cgtgtggccg atcccgatca gcccgttaag tggttcggcc gcgagacgga
      181 gctccaaatt cttaagcttc ccgctcaccc gtctgcgggt cattcggccc atttctaaag
      241 tcgctatgcc agcggaatca taccccctat attcgagccg cttcagggcc tccaatatcg
      301 agccggcgac agggcccttg ccaagtattg caacgatccc gcacattaaa caccgccttt
      361 cttgttgctg ctggctagcg ggttctccgg cccattcatt attgatccag gctccttctt
      421 acgcgcctgc cggtactgtt tgcccctgcc gggcttttcg acttggcgcg cgcgcccaag
      481 cgccattgcg tcgggcgcaa catcctgagt aatcacggac ccagaaccga tataggcgcc
      541 ggcaccaatc ttgatgggag ccactaaagc gctgttcgaa ccgatgaaca cattctcgcc
      601 aacttccgtc cggtatttgg ccaccccgtc ataattgcac gttatcgttc ccgcgccgac
      661 attggtccta gcaccgatgc tggcgtcccc aatgtaactc aaatgattga tttttgcgcc
      721 ttggctgaca tcggagccct tcacttcgac gaaattgccg atgtgcacgt cttgttcaag
      781 agcggtgccg ggacgcagcc tggcgaaggg gccgatgaaa gcgttggcgc ctatggccgt
      841 cccttcaaga taggaaaacg aacgaatgcg ggtgccttcg ccgacacgaa cgccaggacc
      901 aaaaaccaca tggggctcga tgacggcgtc gctgccgagc atcgtgtcaa aggccaatgt
      961 cacgctacca ggatcaatca aagtcgcgcc gcggcgcatc gccgccgtgc gtagacgcgc
     1021 ctgcaagatt gattccgcgg cggcgagctg cgccctgtca ttgaccccta tcacctcgta
     1081 ctcatcggca atttcggcat caaccgctaa accacgcgat cgcgccacgg caacgacatc
     1141 agtcaagtaa tattccttct ggttgtttgc gttgtcgata gcttcgagaa gcgccaaagc
     1201 gcgccgccca tcgagagcca tcaggcctgc gttgcaaatc tcgatttccc gctcttcctc
     1261 ggtcgcgtcg cggtcctcgc ggatggcttt gagctcacca tcgtccaata tgagccttcc
     1321 atagcccgcg ggctgtcttg ccttaaaccc caaggcaaca accgcctttt caccagttgc
     1381 aagcttctta cgcatagtaa tgaacgtatc cgggcgcaca agaggcgtat ccgcaaagac
     1441 gataagcagg tcgtcatagc ctcttgcgat cacttcgcgc gctgcaagga cggcatgtgc
     1501 cgtccccagg cgttctttct gaaccgcgat ctcggcatcg cgcagggata ccttggcttc
     1561 cgcgataacg gcttcatggt tcggtccaac gacaacggcc acttgcccgg cacccgcttc
     1621 ccgaagtgcg ctcaaaacat gggcgagcat cgtcctgcca gcaagctcat gcagaacctt
     1681 gggccgggtc gagcacatcc gcttgctgtc acccgcagca agaacgactg caagacacgt
     1741 acgggatgaa gagccgtcgc ggctgtcgaa agcattggca cccatgattc tagcatctcc
     1801 tgaaggcggc ccgcgctcaa ggggggatca atatttgatc ttataaggat aagtttatga
     1861 aatttcattg ccgtatcccg gcatgaaacg gctgggcgct aaagtctagc tatttcggtc
     1921 taccgccaac ctcaaaatgg tcgctaggca ctttccgtcg cgcttatcct gcaccctttt
     1981 gaggcgacag gcgggactaa gcctttccgc ctaacgacgg tctcgagaaa tcccaatttc
     2041 gcgcttgata ctcgcgtcgg caaagacttt cgcttgggag aacgaactga cactgctatg
     2101 tgatttgctc cccgcgagcc tatccacccg atcgccgaaa aggttacctg aaacgggccg
     2161 ataacggcgg gcggaatctc atccggactt cgccaatgcg ataacccttt agcagtgtaa
     2221 atacgtggcg cgtgtcccaa cggtctccaa atccgatcgc tcaatcgccg ccttagtgct
     2281 cgcgaatagt gcccaccctg tcacacagaa ttgaaccagc ggcgcggttt aaaggcctag
     2341 ccccctgcat ttgtgcatcc tgggttcacg acggcgcagc taccggaatc gagatccaaa
     2401 cacggtaacc gagccatgac aagacggtga cggaatatcg tttgacagca tatgaacgga
     2461 atgactggac gcttatttct gcgcgttttc ccttcgtgca ggcgtttgag ctgcgatcaa
     2521 aaaaacatca agatggaacg taccttcgcc gaattatcaa ctatagagat gaaacggaaa
     2581 gattttttca gcgcggcgca aatagatgcc ggcaaagatg gcagcatggt caagcgtagg
     2641 gaggtcttaa aattcgccct tggcgggata gccggaggga tcacggctct gtcccgcatt
     2701 tgcttggatg aagctatcgc aatgccctcg atcgcagagg aggcgctccg gaccttccct
     2761 ttgtccgaag cggttccgtt tgatccaaac atggtcccac cggccgccag aaccttgtcg
     2821 aagcaagcct ttaaaccgct tccagacgat ctgccgcagg tgttgcgcgg cattagctat
     2881 gaccaatatg cggcaatccg tccgcgcccc gaaaccgcaa tatgggcgac agacaattcc
     2941 ggctttgcgg tcgagccctt gcaccgcggg ttcatctatt ccatgccgat ggagatcaat
     3001 ctcgtcgagg atggaaaagc ccgccgggtt atttacgacg ctgtgctttt tggcacggga
     3061 acggttgagg tccctaagaa ttccggcgac ttggggtttt ccggttttcg cgtcctcagc
     3121 cgtcgcccgg aaggcagttt cgaagtcgcc atcttccagg gagcaagttt cttccgcgcg
     3181 gtggcacccg gccagacgtt gggcaccatg gcaagggcca tgtcgatcaa gaccgccgat
     3241 ttgcggggcg aggaatttcc ggcaatccgg actgtatgga tcgaacgtcc aacgcttacc
     3301 gcaaacgcgc ttgtcattca cgcgctgatc gattccgaaa gcctgaccgg cgcctatcgc
     3361 tttaccctcc ggcctcgcga agcgacaatt attgatacgg aatgtactct tttcgccagg
     3421 gttcccgtcg acagcctcgg gcttgccaca atgagcgcca cgcatatctc gggatcgatc
     3481 gatgagcgcc gcaataatga tcttcgcccc agcgtcagcg aggtgggcgg ccttcagatg
     3541 ctgaccggaa agggggaatg gctgtggcgg ccggtggcca accgcgatac tttgcaagtt
     3601 tcgactttcg tcgatgacaa tcctcgcggc ttcggcttcc tccagcgcga ccggaatttt
     3661 gaccattacc aggacgacga acagcgcttt gaggctcgcc cctccctctg gatcgagcca
     3721 attggcgatt ggtcggctgg gggggttcaa ctcgtcgaaa tcccctccga ctcggaagca
     3781 aatgacaata tcattgcgtt ttggaagccg aagcagccgc tgtctgccgg aagtgagaca
     3841 tttttcgcct accggcagtt ctggtgctgg aacccgcccg agcaaccccc tctggcggtt
     3901 tgcatccagt cgcgctcggg ccgcgggttg tcaccaaaac gccgccgttt catagttgag
     3961 ttcgcaggag acatcttgag caacccgcag aatgccgcgg ccatgaagcc caatttaacc
     4021 ttgtcacagg gatcgataac cctgcttcga acatttacct cggcgaacca gagaacatgc
     4081 cggctcttat tcgaactcga tttccctaac caagactatg cagaaatgcg gctagttttg
     4141 gaagcggcag gcacccccat aagcgagact tggctttatc gatggacgcc gtgaccagcc
     4201 cgcagccgaa cgagaccgcc gtgatggagc tgcgcctggc cgagagcgca cggacgggcg
     4261 attcctacgc agataccccg cccgaagccc ccctcgcgat gccggtgcag agcctctccc
     4321 agttcgactg caccgggcgg catgaagata ttgccagcca cagcctcatt ggctgttggc
     4381 tgacgcgcct ccttgtgttc ggcggcggct tggccttgac cgtgtacggc ggccacgaaa
     4441 tgtatcgcgt ggtcgaggtc ggcggcgtga cggcgcttga atgggctttg ctttttcttt
     4501 tcgtcgccaa tttttcctgg atctcgctcg cctgcacaag ttcgctcgcc ggcttcctat
     4561 ggctcgcgct tgtcgcccct aaacaaccga agccgccgga cgcattgcgt catcgcactg
     4621 cggtcgtcat gccaatctac aatgagagtc ccgcgcgcgt ctttggcgct gtcgccgcga
     4681 tgatcgagga agtcgaagca agcggtcttg gccgcgcgtt tgactggtac tttttgtccg
     4741 acacgaccga ccccgaaatc ttcgtcgccg aggagcaact tttcctcgcc gtgcgcgaaa
     4801 agcttgcttc gggccgccgc ctctactacc ggcacagact gaagaatatc aaccggaagt
     4861 ccggcaatat cgaagatttt gttacgcgct ggggcgggcg ttatgaacac atggtggtgc
     4921 tcgacgccga cagcttgatg gccggccaaa ccatcgtcgc cctcgccgtg gccatggaag
     4981 ccgatcccga ctcaggcatc attcaaacac tgccgctgat cgtcaatcgc aacaccctct
     5041 ttgcccgtct tcagcagttc gcggcgcgta tctatgggcc ggtgattgct gccggtgtgt
     5101 cgatcaccat ggggcgcgac ggcaattact ggggacacaa cgcgatcctc cgtaccaagg
     5161 cgtttgccgc ccattgcggc ttgccggtgt tacgcgggcg ccctccgctt ggcggccata
     5221 tcctcagtca tgattttgtc gaggcggcct tgatgcggcg cgccggctat gccgtttaca
     5281 tgctgccgaa cctcggcgga agctatgaag aatgtccccc ctcccttatc gatttatcaa
     5341 ccagggaccg gcgctggtgc cagggtaacc tccagcattc gcgaattctg cccgccaaag
     5401 gcctgcaccc catgtcacgg cagcatctca tgacgggaat catggcctat gccgcctcgc
     5461 cgctttggat ggcgcagctt atcgtcggta tcgtgattgt cgtgcaggcg agctacatcc
     5521 ggccagaata cttcccgagc gacttcaccc tgttcccggc ttggccgagg ttcgatcccc
     5581 aaagatcgct cgaactcttc gcgctcacca tgacgattct cctcctgcca aagatttttg
     5641 gactgatcct agcattgatc cgaggagtga cccgacgcgg cgcgggcggc gccttccggc
     5701 tcgtcgtgtc ggcgatcttt gaagtcataa tgtcagcgct tctcgctcca atcatgatgt
     5761 tgatccagac gggccatgct atgcattttg tgtttggctt cgatacaggc tgggatccac
     5821 agcgacggga cgacggttcc attcccttta aggccattgt tgcgaggcat ctctcgcatg
     5881 tggcgatggg ttttctaacc ctcatcgccg ggcttttgat ctccccatcc ctcgttggtt
     5941 ggatgtcgcc gactatcctc ggtctcatct tggcgatctt gttgtcgtgg ggtaccggac
     6001 ttcgcagcgt cggccttgcc ttgcgccacg cgaaactcct gatgactcca gaagaacatc
     6061 aggctccggc ggtcgttgcc cgcgcgaatc aattggctga agatttcgcg gaccttgcag
     6121 ccgggcattt gaagggcctt tacgcgcttc atggcgattc ccagtaccgg gccttccact
     6181 tggcctgctt gccgcgccgt cccaaacgat ggaacgggaa tatgtctgcc gaatgggcac
     6241 ttgccgatgc caagatctcg gacgccgaaa ccattgacga agcgatctcc tggcttcacc
     6301 ccaaggaacg catggcaata ctgcaagatc cggcgcttat agctcgcttc gcgtccttac
     6361 caagcttgcc tccttgttcc gacccagtcg cagcatctgc gggatagtct ccgcacgcgg
     6421 gcaggaggcc tccctgggcg aagagcgcta ggccggacgc ggcacgtaag acgaaggttt
     6481 ttgcggcacg ctttactcga gcaaggcgcc gcacctcgcg caaatactta agcggtttat
     6541 ctccctgcgg taaatcgtcc ggcagccggg cgcggcctgc ggaacgctct tttttccgca
     6601 acaaatgaga tccgccgcat atgagaatgg ggcgcgagat tcgaaggcgc gaggcaatcc
     6661 tccgttgcca aagggccggc ggcgatgcgc ctcgagcggt ttcaggttga gtggccacac
     6721 tcaaccgttt cgaaaccccg ctctcattca aagtgttaga gcattttcgg acgacgaacc
     6781 ggtgcccact tcgcctgaaa cgctccagaa tgaggatccc ccaaagcctg cccggcagca
     6841 tcggcaacgc gattcgccta cggagctgcg tcaaaatagc catttagagc gaaagggcag
     6901 tccgaagaaa cgtaatcttc cacctgcggt gcagagccgc gacgaccgtc atacacttgc
     6961 aacatgaagt tgcaattgct gctgtttacc tgaggaatca caattccggc ggcgaaagga
     7021 tgacaaagag cccgctccct gtgccagcca tctggaacac ttggctgtcg cgaacgggat
     7081 ttggatcgtt gcgcgggcgc aatgccgggc gacgggtgat cttagagctt tataaggact
     7141 gctggcttat gatgcctgcg gccgtgggag aggaaaaatg agggaagagg tcaagaaaga
     7201 cacaattgcg aaggcctatg cgcgctgggc gcccgtctat gatcttgttt tcggtccggt
     7261 tttcgagcgt gcccggcgcg ccgccatcgc ggcagcggag cgcgcaggag gccgaatcct
     7321 tgaagtcggc gttggcactg gcctctccct
//
LOCUS       DATMPU010000309         3404 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_2390538,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000309 DATMPU010000000
VERSION     DATMPU010000309.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 3404)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3404)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3404
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2390538"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            209..1582
                     /locus_tag="VNY06_07055"
     CDS             209..1582
                     /locus_tag="VNY06_07055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012383905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HWX14595.1"
                     /translation="MFGLRLPRAAALLIAAAFLIIALLLLGLYLWSPRATLRITTGPT
                     GGIAQRFISAFVSVTTKLHPNIRFETVPVADLEASSTAMEDGRVDIALVRSDVKPPVN
                     GQTLVILRRDVVAIVLPAKSPIKNITNLLNKAIGVPAGPLQDYDSRALDTILGFYNIS
                     PASVKREFLPVSEIGAAIQHHKIVAALAVGPIGPGNVVDVVASIAKATKGAPKILEID
                     EGGAIAKQFPGFESIEIPEGAFRGHPPTPDDTVKGLAVSYRFVVPLRMLNIVAAAIAR
                     SIIKTKSNLMAVTPQANQIEAPDTDNENPLLPVHPGVAAYLTNGDQSFFDQLQQYFYV
                     VGIPLSVGASLAALFTGHWRSRKLEEDQRQIFRLLVIADEAARAGLSELEKLEVEFRM
                     IVASLVNKLAAGQGSADQLPLSLAVEHARHSIEGRRMLLSGSTARIAESSDAALNDLL
                     PASGQPI"
     gene            complement(1609..2784)
                     /locus_tag="VNY06_07060"
     CDS             complement(1609..2784)
                     /locus_tag="VNY06_07060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016921611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HWX14596.1"
                     /translation="MLAISISVLSACSTFDHYAAVPPGETYKATILSLSNARFFIDRP
                     AAISAEQERALVREAKALGIGRGGTLPAAHILSLSGGGDNGAFGAGLLVGWTAHGDRP
                     KFKLVTGVSTGALIAPFAFLGPEYDAALTDVYTNINPSKVYEKRFILAALTEDALAST
                     VPLYETISHYVDADLLAKIAAEYQKGRLLIIQTTDLDAGIPILWNIGAIAASGRPDAL
                     DLVRRILLASASIPAAFPPVMFDVDVNGQPYREMHVDGGAVSQAFLVPPSVNIHVAQE
                     SAGYRRSSVVAYVIRNSRLRTDWSDVERLTLPIAARAVSTLINYNGVGDLYRIFLVTD
                     RAGAKFNLAFIGDEFHADHKEDFDQNYMRALYSYAYEKASKGYPWDHAPPGFAPTRH"
     gene            3186..>3404
                     /locus_tag="VNY06_07065"
     CDS             3186..>3404
                     /locus_tag="VNY06_07065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX14597.1"
                     /translation="MFQIEFVGPSREGAPAEVLDRMTTDMISLPQVVAHAKSLFSNVI
                     VQRTHKPVPHGFRILDNQGSEVAHWSIDD"
BASE COUNT          745 a          900 c          980 g          779 t
ORIGIN      
        1 gggcgcgggc gatatcgccg aaggttttgt tatagcggtt gagaaatgcg ggctggtgct
       61 ggccgagcat tttccgcttg aaccggatca agagcagcag cttcccgacc ggatttattt
      121 gatttaagcg cgccttggat agggaggact gctttcgctc aaaatctgct cttgtcagtg
      181 acagcaagtg ccttggaggc atattgagat gttcggactc cgcctgcccc gcgcggcggc
      241 tctgctgatc gctgcggctt ttctcatcat cgcgttgttg cttttaggcc tttatctttg
      301 gtctccgcgg gcaactttgc gcattaccac cgggccaacg ggcggcatcg cacagcgctt
      361 catttcagcc ttcgtctccg tgaccacgaa acttcatccg aatatccgct ttgaaactgt
      421 tccggtggcc gatctcgaag caagttccac ggccatggag gacggcagag tcgatatcgc
      481 gctcgtgcgt agcgatgtga agccgccggt caatgggcaa acgctggtca ttctaaggcg
      541 cgacgttgtt gcgatcgttc tccctgcgaa gtcgccgatc aaaaatataa ccaatcttct
      601 gaacaaagcg attggcgttc ccgctggacc acttcaagac tatgattcaa gggcgcttga
      661 tacaatcctc ggcttttaca acatttctcc agcgtcggtt aagcgagaat tcttgcccgt
      721 ttctgaaatc ggtgctgcta tccaacacca taagatagtg gccgccctgg cggttggccc
      781 aatcgggcca ggtaatgtcg tagacgttgt ggcttcgatt gccaaagcaa cgaagggggc
      841 tccaaaaatt ttggaaatcg atgaaggggg cgcgatagcg aagcagttcc ctgggtttga
      901 atccatcgaa attcctgagg gtgcctttag aggccatccg ccaacgccgg acgacacggt
      961 gaaaggcctc gcggttagct atcgtttcgt ggtcccgctc agaatgctga atatcgtcgc
     1021 cgcggccatt gcgaggtcga taataaagac caagtcaaat cttatggctg taacgccgca
     1081 ggccaaccag atcgaggctc cagacacaga caacgaaaac ccgctcttgc cggttcatcc
     1141 tggggtcgcg gcttacctca cgaacgggga tcagagtttc tttgatcaat tgcagcaata
     1201 tttttatgtc gtaggcattc cgctgagtgt cggcgcatcg ctggcggcgc ttttcaccgg
     1261 gcattggcgt agcaggaagc tggaagaaga ccagcgacag atctttcggc ttcttgtaat
     1321 tgctgatgag gcggcgagag ccggcctttc agagctcgaa aaactggagg tggagtttcg
     1381 catgattgtc gcgtctttgg tcaacaaact cgctgcagga caaggttccg ccgatcagct
     1441 tccgttgtcc ttggctgtcg agcacgcgcg gcattctatc gaggggcgga ggatgctttt
     1501 aagtggaagc actgctagaa tagctgaatc gtctgacgcc gctctcaacg atctattgcc
     1561 cgcaagcggt cagccaatct aaattaaccg cagccagctc tatattagct aatggcgggt
     1621 gggcgcaaaa cctggcgggg cgtgatccca agggtagcct ttgctggcct tctcgtaagc
     1681 atagctatac agcgcgcgca tatagttttg gtcaaaatct tccttgtgat cggcgtggaa
     1741 ttcgtcgcca atgaaggcga gattgaactt cgctcccgcg cggtcggtaa ccaaaaatat
     1801 gcggtagaga tcgccgacac cattgtagtt gatcaaggtg gagacggctc gcgcggcgat
     1861 tggtagcgtc agtctctcga catcgctcca atctgttctt agccgagaat ttcgaatgac
     1921 ataggcgacg acggagcttc tgcggtatcc ggcgctttcc tgggccacat gaatgttcac
     1981 gcttgggggg acgaggaaag cctgactgac ggcgccgcca tctacatgca tttccctata
     2041 gggctgcccg ttgacatcga cgtcgaacat gacaggtggg aatgcagcag gaatcgatgc
     2101 cgatgcgagc aatatgcggc gaacgaggtc aagagcgtct ggacggccgc tcgcggcgat
     2161 tgccccaatg ttccacaaga tgggaattcc tgcgtcgaga tctgttgtct gtatgataag
     2221 gagacgccct ttctgatatt cggccgcgat tttggcgagc aaatcggcgt cgacatagtg
     2281 ggaaatggtt tcgtaaagcg gcaccgtact cgcgagagcg tcctcggtga gcgcggcaag
     2341 gatgaaccgc ttctcataga ccttcgaagg atttatgttg gtatagacgt cggtgagtgc
     2401 cgcgtcatac tccgggccga gaaaggcaaa aggcgcgatg agtgcgccgg tgctcacacc
     2461 cgtaacgagc ttgaactttg ggcgatcgcc gtgcgcggtc caaccgacca gaagtccagc
     2521 gccgaatgcg ccgttgtcgc cgccaccaga gagcgacaga atatgcgcag ccggcaaggt
     2581 gccgccgcgg cctatcccga gagcttttgc ttctcgaacg agtgcgcgtt cttgttccgc
     2641 ggagatcgcc gctggcctat cgatgaaaaa gcgcgcgttg gaaaggctaa gaatagtcgc
     2701 tttgtacgtt tcacccggcg gaaccgccgc gtaatgatcg aaagtggagc aggcagagag
     2761 gaccgaaata cttatcgcaa gcacgcctgc attcacgcga ttgcgcgccc gtgcgactaa
     2821 gcttgttagg ctcctcattg cggactcgtg cttgctaatc gatcgtcatt tccgttgcag
     2881 caacattgca ccgcaacggc acaagcataa ttctgccggc gggtcgtgac tgcccccacg
     2941 aaagcaaatc tagcaagctt ctcgcgtcgt ttcgataaga tgaggtaaac cgccggaacc
     3001 gcgaagcggt ttgtcaatcg cagtggcatt tgcgccacag caggaactgg ctataacctt
     3061 gcgccgtttt gtgaggaaca gcgtttagca ccggtgacgg aatattcttt tttacggttg
     3121 ccttcgctgt cggcagccct aattgcagcc acaaatggaa acgtttgacc gcggggaggc
     3181 cgttaatgtt ccagatcgaa tttgttggtc ccagccgcga aggggccccg gccgaagtcc
     3241 ttgatcgaat gacaacggac atgatcagtc ttccccaagt ggtcgcgcat gcaaagtcgc
     3301 tcttctctaa tgtcattgtc cagcggaccc ataagccggt accccacggg ttccggatat
     3361 tggacaatca gggaagcgag gtcgcgcatt ggagcattga tgat
//
LOCUS       DATMPU010000310         2495 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_3337465,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000310 DATMPU010000000
VERSION     DATMPU010000310.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 2495)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2495)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2495
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3337465"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..185
                     /locus_tag="VNY06_07070"
     CDS             <1..185
                     /locus_tag="VNY06_07070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006020947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX14598.1"
                     /translation="SEYAKTSFEGIVAQATKMGELYSNLAKLAFKPFGGAIANSQVSA
                     ETPPASERNTPMNSKI"
     gene            362..562
                     /locus_tag="VNY06_07075"
     CDS             362..562
                     /locus_tag="VNY06_07075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX14599.1"
                     /translation="MHALRFGRKLSLPSTIAIKHELRKLMADDQVKKAEAEARAESTD
                     IPQMNVGHDLQKKPTDSPPMSE"
     gene            948..2038
                     /locus_tag="VNY06_07080"
                     /pseudo
     CDS             948..2038
                     /locus_tag="VNY06_07080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012383550.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
BASE COUNT          534 a          719 c          676 g          566 t
ORIGIN      
        1 agtccgagta tgcgaagacg tctttcgagg gcatcgtcgc gcaggcgacg aagatgggcg
       61 aactctattc caacctcgcc aagttggctt tcaagccgtt tggaggggcg atcgccaact
      121 cccaagtctc ggcggagacc ccgccagcct cggagcgaaa caccccgatg aatagcaaaa
      181 tataggacca caaagttgaa tcatcctgct ctccattaca tcaatgaaca ccaatccgac
      241 atacggggca tcaaggatgg ctggtatgca atggaggaag acggcaacct tgttgcgggc
      301 ccgttttcca gccgcgagga ctgcctaaag tatttgaccc aattacacac aggaggccca
      361 aatgcacgcg cttcggtttg gacgtaaact cagtcttcct tcgacgattg cgataaaaca
      421 cgagctcaga aagctgatgg cagacgatca agtcaaaaaa gcagaggcag aggcaagagc
      481 tgaatcaacc gacattcccc agatgaacgt cggccatgat ttgcaaaaga aacccactga
      541 ttctccgcca atgagcgaat gacgcctcga cacgtcgcaa cccagcagga tttcgagatc
      601 aatccgagag cacacaggct cgaataacgc gaaagcagcg gcgaggctgg cagggccgca
      661 gctcgcaagc ctgtaaaaac ggcggccgga gatttgcaat gtatgatcag atcttagtat
      721 cggtttttct ggctttgaca cgttaagcgg ttttacttgt caagcgtctt aatcggccac
      781 catagcatca cagggaaatc aacggcttgt acgagcacca taacgccaaa atggcctcaa
      841 gtccgcctta ctgagttaac gtgtcaatgc cgtattttgg taccgagctg gcgctcacga
      901 ccccgaaggc gtaaattctc tttcgaaatt gagcccgagg aagtgtcttg tcctggtccc
      961 taacgatcgc ccgctttggc gcaacgatcg tgcgcgtgca tctcaccttc tttctcttcc
     1021 tcgtttggat tggcgtcagc gcttggcagc agggagggtt ttccgcggcg cgtaacagcg
     1081 tgctgttcat catccttata ttcggctgcg tcgtctgcac gaattcggtc acatccttat
     1141 ggcccggcgc tttgggatcg agacgccgga tgtgattctg ctgcccatcg ggggagtggc
     1201 gcgcatgccg cgtatgccgg aataccccgc ccaggaactc gcggtcgcga ttgcgggccc
     1261 gatggtgaac atcgtcatcg cccttttcct ctttctcgtc cttggcacgt ttcaatccga
     1321 gtccttggcg catattgacg atccgcgtgt ctcgcttctt gcacggcttg ctgccgtcaa
     1381 tatctttttg gttcttttca acttgatccc tgcttttcca atggacggcg gacgtgttct
     1441 ccacgctatc cttgcgatga aaatcggaaa cgccagggct acgcagctcg cggcctccct
     1501 ggggcaagtc ttggcatttg cacttggctt tctcggtctt tttggcaacc cgctcctgat
     1561 tttcattgcg atcttcgttt atatcgctgc cggaggagag gcgcagatgt ctgccttcaa
     1621 cgaggcggcg cgcgggcttt cggtgggcga cgcgatggaa acccgcttca ctgcgattcc
     1681 ggtcgaggca actttggctg acgcgatcga gactctgctt gcggcggcgc agcatgaatt
     1741 cccggtagtc gatgctttcg gaaagccggt tggcctcctt acgcgagagg acatactatc
     1801 ggccctaaag aaccacgacc gcgaggcgtc aatcacgaca tttatgcgcg ccctcgagac
     1861 cgtggggagc aagacctcgc ttgtggcggt gctggatcga cttcatggac ctcaagccgt
     1921 ggcgctcgcc gttaccgatc cggatgggat cctcgttggc ctgctcacgc gccagaacct
     1981 tggtgagatg atgatcatca aggccatgcg gcccgactgg cgtttcgatc gcagctgaag
     2041 gggttccgct gtaacgcagc atccagggag attatatctc tgcccgcgcg agccgaccca
     2101 ttcgccgtgt gagcctggtg attacatcga actttgcaac gatgtttcgt ggcttaattt
     2161 cccgcctcat agttgagcga catggaacgg gaagtgaccg ccgaatatga gcctaccgcc
     2221 gatcagcgcg ccctcgtcga gagtgctcgc tcgtgcttgg aatcatgccg cctggaaacg
     2281 ccatcaacat aacggctatt gcccatcatc agccgatcgc agactcgtca aagctagggt
     2341 ttttcagcaa actgctaaag catcgcctcg cgctctagct ccacctcggc ctgcaggctt
     2401 gcgagatcgc ggaatatcga agcgaccgcg agcacccggc ccttgcgctt gtatttcagc
     2461 aaacaatctt tcgcctggat atcgccgtcg atcga
//
LOCUS       DATMPU010000311         4738 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_3050397,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000311 DATMPU010000000
VERSION     DATMPU010000311.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 4738)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4738)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4738
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3050397"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..800)
                     /locus_tag="VNY06_07085"
                     /pseudo
     CDS             complement(1..800)
                     /locus_tag="VNY06_07085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007815455.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
     gene            complement(875..1135)
                     /locus_tag="VNY06_07090"
     CDS             complement(875..1135)
                     /locus_tag="VNY06_07090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX14600.1"
                     /translation="MKKGYALAYLAALALVAAGSLFSIAASAEDKAKPKSTSQEKMMP
                     ETAKPEAKKPKDADPDAMKSTDPKSEDSESDQMGGDDMGTQD"
     gene            complement(1295..1750)
                     /gene="msrB"
                     /locus_tag="VNY06_07095"
     CDS             complement(1295..1750)
                     /gene="msrB"
                     /locus_tag="VNY06_07095"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007612345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HWX14601.1"
                     /translation="MFATAAGGIVLPAWLLANADELEVTKTDAEWRSLLTPAQYDVLR
                     QEATESPGSSPLLHEHREGIFACAGCDLPLFKSETKYDSGTGWPSFWGPIDGAVRTRS
                     YRKPWMERTEVHCRRCGGHLGHVFKDGPPPTGLRYCMNGLALKFVPKSS"
     gene            complement(1790..2527)
                     /gene="msrA"
                     /locus_tag="VNY06_07100"
     CDS             complement(1790..2527)
                     /gene="msrA"
                     /locus_tag="VNY06_07100"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011427870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HWX14602.1"
                     /translation="MQNHLFQLGRRGTGAQQRFIRLGLWLTILASLARPVAAESQTRI
                     DPPLTDVTQPAEGGTEKAVFAGGCFWGVQAVFQHTKGVTGAVSGYAGGTLANPSYTQV
                     TSERTGHAESVEVTFDPKQISFGRLLQIYFFVAHDPTQLNRQGPDEGSQYRSEIFTTS
                     IGQQNVAEAYIAELDKAHAFPGPIKTKVEPLRAFYPAESYHQDYATLHPASTYTVVND
                     LPKIENLQKLYPELYRERPVLVGDIGQ"
     gene            complement(2834..3958)
                     /locus_tag="VNY06_07105"
     CDS             complement(2834..3958)
                     /locus_tag="VNY06_07105"
                     /inference="COORDINATES: protein motif:HMM:NF024110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWX14603.1"
                     /translation="MNVFSFRRFAALLRKEWLQMLRDPITLRFIIALPMMQLFLFGYA
                     INSNPKNLPTGLLSVEHSKYERTLIAALRNTGYYDVSVLSSEAEAERGLAQGEFLFVI
                     NIPPNFDRSVDRGEAPSVLIDADASDPTAIINATAALAALSAALNRDLPPVRQKEPAP
                     EPFQFVVHARYNPEQLTVLNIVPGLICIVLIFSTVFVTALSITRERERGTMENLLAMP
                     VRPIEVMLAKIVPYVFVGYVQVVLILLISAFVFEMPVRGSIILLLVSLGLFIAGNLAL
                     GITFSTVSENQMQAIQFAQFTLLPSIFLSGFMFPFKGMPVWAQWAGEVFPTTHAIRIV
                     RGLLLKGNRLPEILPELWPIAFFTLVVVMVAIRFYRETLD"
     gene            complement(3958..>4738)
                     /locus_tag="VNY06_07110"
     CDS             complement(3958..>4738)
                     /locus_tag="VNY06_07110"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWX14604.1"
                     /translation="KTTTIRMLCGLLVPDGGSGKCLGHDIIREASLIRREAGYMTQKF
                     SLYEDLTVFENLDLVASVYEISNRRDAVFEIMDRMGLARRDQLAGHLSGGWKQRLALA
                     ACVLHQPKLLLLDEPTAGVDANARREFWDMIHDLAGEGLTVLASTHYMDEAERCKSVV
                     YLANGRIVVQGAADDIFARSGLVIFEATGGDLDAAARRLRRTSGVEAAAVFGHALRVA
                     GTDRAALANALQSTGAALSWNEVEPRLEDVFIHMLSVKEET"
BASE COUNT         1060 a         1356 c         1353 g          969 t
ORIGIN      
        1 cgaacgcagc tcattccttg gcccagctag aatcaccatt acgacatgca caaagacgaa
       61 gagcacgatc aggctcgccg ctatgaaatg gatcgtgcgg gccgattgcc ttccgccaaa
      121 caagtcgacg agccaaggaa agattgcatc catccccggc gacatggtga gtccggtcaa
      181 accatcgccg gtagcagcac aaagatgacg gccagataac tcaacttttg cagcggatta
      241 taatggcgtg ccgcatcccc acgcgggaat ttcagacgca ggtgatcggc gatatcgcga
      301 aagagcgagc tcgggcgcaa ttgattgggg gccgggagaa ggtcccagcg aagatgcccg
      361 ctcacaatag cggcgaggag atagagcaag aggttagcag cgaacagcca ggcgaaaaag
      421 aaatgccatc ttcgccccaa tccaaggtcg cgccagcttg ggacagtcat ccatcgcgga
      481 aatccacgca ccacttcctg gccattggca ttgcgcgaaa ccccaaaaat ccctgtcgtg
      541 tcaaaggttg atgcaccaac cctagtgaac cctgcggggt tgcgctttgg accatgtgcc
      601 ccgacctcaa atagcgcctg gctaggattg gcgccggaat gtccccaata aagggccgga
      661 tgggcattga aaatctgcag gccgctcatc aaaagcaaaa ttatgcagag caagttgatc
      721 cagtgagtca ctcttgtaat caggctgtgt cgggatatga gcaaggcacg cgggagctgc
      781 ggcgacggag tttcgctcat gacctgctcc tgcgttgcgt ggccctctgc gtccgctccg
      841 gtgcgagtcc cggagcggac ggcatttgct cggtttaatc ttgtgtaccc atgtcgtcgc
      901 cgcccatttg gtcggactcc gaatcttcgg atttcggatc ggtggatttc atggcatcgg
      961 gatccgcgtc cttgggcttc ttcgcttccg gcttagcggt ctcgggcatc atcttctcct
     1021 ggctcgtgct tttcggcttc gctttgtctt ccgcagacgc cgcaatggag aacagggatc
     1081 cggcggcaac caaggccaga gcagcaagat aagcgagggc atatcctttc ttcatgtttt
     1141 gctccttttc ataatttgcg ccctatttgc gcattgttcc ccgtcgcacg caactgcacg
     1201 ccggagcggc gccatggaac gccgtagctg cggtcaaagc tctcactggg ttttgaagct
     1261 tgctcgagag gttctgaccg ctgtggccaa tcccttaact cgatttcggg acaaatttaa
     1321 gggcaagccc attcatacag tagcgcagcc cggtgggtgg cggtccgtcc ttgaagacgt
     1381 ggcccagatg cccgccacaa cggcggcaat gcacttcggt tcgttccatc cacggctttc
     1441 tgtatgacct tgtgcgtact gctccgtcga tcggacccca gaaactcggc cagccggttc
     1501 cgctgtcata tttcgtttca gacttgaaga gcggcaggtc gcaaccggcg caagcaaaga
     1561 ttccttcacg atgctcgtgc agcaacgggc tgctgccagg cgattccgtg gcctcttgcc
     1621 ggagaacatc atactgggcg ggagtgagga ggctccgcca ttcggcatct gtcttcgtca
     1681 cttccaattc atccgcattg gcgaggagcc acgcgggaag cacgatcccg cccgctgccg
     1741 ttgcaaacat caccaaaagg tcacgccgca gcattctctc aactcctcct tattggccga
     1801 tatcaccaac gagcacaggt cgctcgcgat agagctccgg gtaaagcttt tgcagatttt
     1861 cgattttcgg caggtcgttg acgacggtat aggtgcttgc agggtgaagc gtggcgtaat
     1921 cctgatgata ggattccgcg ggatagaagg cccgcagcgg ctcgaccttg gtcttgatcg
     1981 ggcctgggaa ggcgtgcgcc ttgtcgagct ccgcgatata agcctcggca acattttgtt
     2041 gccctatgct ggtggtaaaa atctcggagc ggtactgaga gccttcatcc ggcccctgcc
     2101 gattgagctg cgtaggatca tgcgccacga aaaaatagat ttgcagcagg cggccaaaag
     2161 aaatttgctt tgggtcgaaa gtgacctcga ccgattcggc atgacctgtg cgctccgagg
     2221 tcacctgggt gtagctgggg ttggccagcg tgccgcctgc atatcccgat accgcgcctg
     2281 tcacgccttt ggtgtgctgg aacacggcct gcacgcccca aaagcagcca ccggcaaaaa
     2341 ccgctttctc ggttccgcct tccgcgggct gagtcacatc agtcagcggc gggtcgatgc
     2401 gggtttgaga ctcggcggct accgggcgag caaggctagc taagatcgta agccaaagcc
     2461 ccaaccgaat aaagcgctgc tgcgcgcctg tgccacggcg gccgagttgg aacaaatgat
     2521 tttgcatagt ccttggtcct tgcgcgatcc ctttgaaatg aacaccgaat cttggccgcg
     2581 aggtggccga caaaccctta tacgtttcac aacctcgata ggttacagcc tgcggtggtt
     2641 attggtacca aagatgcgat cacttctgcg tgcgccatct cacgaagcgt gagccaagca
     2701 aaaacaaatc cggcaccaag caacaagaac atgaattcga agaccgcagc ctgtggccgc
     2761 ccgatgggac gacagtcttg cggtgactgg caaatattat tggcgggaac gcagccacgg
     2821 tcattcaccc gtgctaatcg agcgtctcac gataaaacct gatcgcgacc atgacgacca
     2881 ccagcgtgaa gaacgcgata ggccacagct ccggcaaaat ttctggcagc ctgtttcctt
     2941 tgagcagcaa accgcggaca atccgaattg catgcgttgt ggggaacact tcgccagccc
     3001 attgggccca gacaggcatg cctttgaacg gaaacataaa gccggacaaa aatattgacg
     3061 gcaaaagcgt gaattgggcg aattggatcg cctgcatctg attttccgat accgtcgaga
     3121 atgtgatccc aagcgcgaga tttccggcta tgaaaagccc cagcgatacg aggagcaaaa
     3181 taatcgagcc ccgcaccggc atctcaaaga caaaggcgga tatcaaaaga ataagtacga
     3241 cctggacata gccaacgaag acataaggca cgatctttgc gagcatgacc tcgatcggac
     3301 gcacgggcat ggccagcaaa ttctccatcg taccgcgctc gcgctcgcgc gtgattgaga
     3361 gcgccgtcac aaagactgtc gagaatatca aaacgatgca gatcaatccc ggcacgatgt
     3421 tgagcactgt caattgctct ggattgtagc gggcgtgaac cacgaactgg aagggttccg
     3481 gcgcgggttc cttttgacgg accggtggca gatctcggtt cagggctgct gatagcgccg
     3541 cgagcgccgc ggtcgcgtta attatggcag taggatcgct cgcatccgcg tcaatgagta
     3601 cggatggggc ctcgcctcga tccaccgagc ggtcaaaatt cgggggaatg ttgatgacga
     3661 atagaaactc gccttgcgcc agcccgcgct cagcctcggc ttctgaggaa agtaccgaaa
     3721 catcataata acctgtgttg cgaagcgcgg cgatcagggt gcgttcatat ttcgaatgtt
     3781 ccacggaaag aagccctgtc gggaggttct ttgggttgct gttgatcgcg tagccaaaca
     3841 gaaacagctg catcataggc agtgcgatga taaagcgcag agttataggg tcgcgcaaca
     3901 tctgcagcca ttccttgcgc aaaagtgcgg cgaaccgcct gaaggagaac acattcatca
     3961 ggtttcttcc ttcacactca gcatgtggat gaagacgtct tcgagacggg gctcgacctc
     4021 gttccaggaa agagcggcgc cagtgctttg gagagcattg gcaagcgcgg cgcggtctgt
     4081 tccggcgaca cgcagcgcgt gcccgaacac ggccgccgcc tcaactcctg atgtgcggcg
     4141 caatcgccgc gcggccgcat ccaaatcacc gcccgtcgcc tcgaaaatga cgaggcctga
     4201 tcgggcgaag atgtcgtccg ctgccccttg cacgacgatg cgcccatttg caagatagac
     4261 gacgcttttg cagcgctctg cttcatccat gtaatgcgtt gacgcgagca ccgtcagtcc
     4321 ctcgccggcc agatcgtgga tcatgtccca gaactcgcgc cgtgcgttgg catcgactcc
     4381 tgccgttggc tcgtcaagga gcaacagctt tggctgatgc aggacgcagg ccgcgagcgc
     4441 gagccgctgc ttccagccgc cggagagatg accagccaat tgatcacggc gggcaagccc
     4501 catccggtcc ataatctcaa agactgcgtc gcggcgattt gaaatttcgt agacgctcgc
     4561 gaccaggtcg aggttttcaa acaccgtgag gtcctcatag aggctgaact tttgcgtcat
     4621 gtagccggcc tcgcggcgga tcaacgaggc ctcgcggatg atgtcgtgtc caaggcactt
     4681 gccgcttccg ccatcgggga caagcaggcc gcacagcatc cggatggtcg tggttttg
//
LOCUS       DATMPU010000312         4708 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_873410,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000312 DATMPU010000000
VERSION     DATMPU010000312.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 4708)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4708)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4708
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_873410"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..523)
                     /gene="pyrB"
                     /locus_tag="VNY06_07115"
     CDS             complement(<1..523)
                     /gene="pyrB"
                     /locus_tag="VNY06_07115"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009491013.1"
                     /note="catalyzes the transfer of the carbamoyl moiety from
                     carbamoyl phosphate to L- aspartate in pyrimidine
                     biosynthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HWX14605.1"
                     /translation="MANPPKAFLFPHRHLLGIEGLSAKDIMLLLDLAEEAVEVSRQVE
                     KKRATLRGRTLINLFFEASTRTQSSFELAGKRLGADVMNMPVASSSIKKGETLIDTAI
                     TLNAMRPDIIVVRHARAGAVHLLAKKVDCAVVNAGDGSHEHPTQALLDALTIRRNKGR
                     IEGLTIAICGDIRH"
     gene            815..1423
                     /locus_tag="VNY06_07120"
     CDS             815..1423
                     /locus_tag="VNY06_07120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX14606.1"
                     /translation="MNTSAKNFFAATVFVLSGFPAVAENKPLTCWYSANADFTSATPG
                     DSGKTMGSVEKSGSGDKTFSYIISARDGTACPVQLPLSTETALTVAVVRQDSESCSND
                     NVNDAGQAPVAGSVTVFATTVNSMTAGVRLVGVSPSTTYRVFLKCGKQLGALRTDAEG
                     NGGRTFNYLTDALGPLGATYAFEITPDGAEAGSELQSLSVKK"
     gene            2200..>4708
                     /gene="topA"
                     /locus_tag="VNY06_07125"
     CDS             2200..>4708
                     /gene="topA"
                     /locus_tag="VNY06_07125"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012592797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HWX14607.1"
                     /translation="MNVVIVESAAKAKTITKYLGKDYDVHASYGHVRDLLAKDGSVDP
                     DSDFVMLWDIDAQSAKRLAEIAKAVKGAKRIILATDPDREGEAISWHVLEVLKEKKVI
                     SGKPVERVAFNAVTKSAILAAISNPREIDAALVDAYFARRALDYLVGFKLSPVLWRKL
                     PGARSAGRVQSVVLRLVCDRELEIEKFVAREYWSITAHLKTKAGAPFVARLVGADGQK
                     VNRLDIGSGEDAEAFRAALERAKFTVANTEAKPVKRHPPPPFTTSTLQQEASRKLGFA
                     PARTMQLAQRLYEGADIGGETVGLITYMRTDGVDIAPEAIASARKLIGSQYGADHVPA
                     APRKYSAKTKNAQEAHEAIRPTDLSRQPRHVAKSLKPDQARLYELIWTRTLACQMESA
                     ELERTTVDILAETESRKLDLRATGQVIRFDGFLKLYQEGSDDEEGEDGGRLPAMAAGE
                     ALGRENVDIKQHFTEPPPRFTEATLIKRMEELGIGRPSTYASTLAVLRDRSYVRLEKK
                     RLIPEDKGRLVTAFLESFFARYVSYDFTAALEEDLDRVSNHELNWKDLLRNFWEDFSD
                     ALAGIKDLRTAQVLDSLNELLGPHIFLPKGDGSDPRVCPSCGLGQLSLKLGKFGAFIG
                     CSNYPECKFTRPLVNADGDGASNGERSGAKILGEDPASGEEISLRQGRFGAYVQQGEG
                     EKPKRASLPKTIEAEALTLAQAIGLLSLPREITKHPETKEPIVAGIGRFGPYVQHGKT
                     YANIGKDDDILRMGANRAIDLIIAKESGLSGRRFGDSASAPSRTLGEHPKGGELVIKA
                     GRYGPYVKYGKINASLPQGADPTTFPLEEAVALVAAKE"
BASE COUNT         1028 a         1317 c         1349 g         1014 t
ORIGIN      
        1 agtgcctgat gtcgccacag attgcgatcg tgagcccctc gatccttccc ttgttgcgcc
       61 ggatggtcag cgcatcgagc agtgcctggg ttgggtgctc gtgggagcca tcgcccgcat
      121 tgacgaccgc gcaatcaacc ttttttgcca agagatgcac cgcgccagcc cgggcatggc
      181 gcacgacaat gatgtcggga cgcatcgcgt tgagcgttat tgcggtatca atcaaggtct
      241 cgcccttttt gatactcgaa gacgcaactg gcatgttcat gacatcggcg ccgagccgct
      301 tgccggcgag ttcgaaggaa gattgcgtcc gggtcgaagc ctcaaaaaag aggttgatca
      361 gggtgcggcc gcgcaaggtc gcgcgtttct tttcgacctg gcgcgagact tcaaccgctt
      421 cttccgcgag atcgagcaga agcattatgt ctttagcgga aagcccctcg atcccgagga
      481 gatgccggtg cgggaagagg aaagcttttg gcggattggc catcggcacc gttctataag
      541 accaaatttg tcttcgctac gaatgtttcg gcgcaacggc gttggctgac caaaaattag
      601 gggaaatttt tgtgggcagg gcgcaaaacg cgccatggcc gtcatggtcc tcttcgaaac
      661 aacacgtggc ctttcagtgg ccggcgtttg ttccgttcgg ctatgttggc cgcgccacca
      721 aaattgtgac atttttttct tgaagcaggt tgcatttttc cgcgcaaagc ggttcaagtc
      781 ggcagggatg aagcttacgc aagatcggac cgttatgaat accagtgcta agaatttttt
      841 cgccgccaca gtgtttgttc tcagcggctt tccggcagtc gctgaaaata agccgctgac
      901 ctgctggtat agcgccaatg ccgattttac ttccgccact cctggtgact cgggcaagac
      961 gatggggagc gttgaaaagt cggggagcgg cgacaagact ttttcttata taatctcggc
     1021 gcgggacggg acggcctgtc cagtccagct tccgctatcg accgagacgg cgctaaccgt
     1081 cgctgtggta cgtcaggatt cagaaagctg cagcaacgat aatgtcaacg atgccggtca
     1141 ggctcccgtc gccggttctg tcaccgtctt tgcgactacc gtaaactcga tgaccgcggg
     1201 ggtaaggctg gtcggtgttt ctcccagcac gacctaccgg gtttttctga aatgtggcaa
     1261 gcagctcgga gcccttcgca ccgatgctga aggcaatggc ggaaggacat ttaactattt
     1321 aaccgatgcg cttggtccgc ttggtgcaac ctatgccttc gaaattaccc cggacggggc
     1381 ggaggcagga tctgagctgc aaagtcttag tgtaaaaaaa tagcctccca actctggaat
     1441 cgggccttgt cgcctggcca agcacgcaga ggttcaccta gctgcgtgcg ccgcaccaag
     1501 ctgcgagcgg tctagatact tttgcagcgg ccttgttccg ggaggcttgc gaactttttt
     1561 cgcctcggct aagttcaaag tgcctcccac gagactgacc ccgtgagagg cggtatggaa
     1621 tttcttaggc cctcacgtgc gtcattgcca cgcgaaagct acggtaatga acacgtgaag
     1681 ggtgccagtt atgtattggc aaaataaaat tatcatccaa aactcctttc tgcccactca
     1741 tgatgggatg atcaaatttt atgcatcgat ttccctcctt gccaagtgag acttgtattt
     1801 tctcctcagg aatggaccat tcctcctccc agggtcgtct aatcatccag ttccattttc
     1861 ccacagagat ttgtgcagga tcgcagcaga tgggtgcgaa agcggcctca gggcgagtct
     1921 gcagccatct tattgcaagt gttctcgatt ttctcccgta atgaaggcac cttaaacttt
     1981 gctaagggta tgcggtaaaa tagccggtcc cgccttgcgg cgccctgccc gattgactaa
     2041 ccgcccgcgc tcccccattt tccaggccgc cgattcaggc taagccccgg ctcacccttt
     2101 tggagaatgg gcggggcggt tcgttatttg gccctgttcc aagccttgcc gcgcgccaaa
     2161 atagaaaggc gcgccatggg gaaactgcgg aatccttgca tgaatgttgt cattgtcgag
     2221 tcggcggcta aggccaagac gatcaccaaa tatcttggca aggattacga cgttcacgca
     2281 tcctacggcc atgtccgcga cctcttggca aaggatggct ccgtcgatcc tgacagcgat
     2341 ttcgtaatgc tctgggatat cgatgcccaa tcggcgaaaa ggctggcgga aatcgccaag
     2401 gcggtgaaag gcgcaaaaag gataatcctc gcgaccgacc cggatcgcga gggtgaagcc
     2461 atttcctggc acgttttgga agttctgaaa gagaagaaag tcatctccgg caagcccgtc
     2521 gagcgggttg ccttcaatgc ggtgaccaaa tcggcaattt tggcggcgat cagcaatccg
     2581 cgcgagatcg atgctgccct ggttgacgcc tattttgccc gccgcgcgct agactatctc
     2641 gtgggcttca aactctcccc cgttttatgg cgcaaattgc ctggcgcacg gtcggccgga
     2701 agagtccaat ccgttgttct gcgtctggtt tgcgaccgcg aacttgaaat cgagaaattc
     2761 gttgcgcgcg aatattggtc catcacggcg catttgaaaa ccaaagccgg ggcgccgttt
     2821 gttgcaaggc tggtcggcgc agatggccaa aaagtcaacc gcctcgacat aggctcggga
     2881 gaggacgcgg aagcttttcg ggccgccctc gagcgggcca aatttactgt cgccaatact
     2941 gaggcgaagc ccgttaagcg ccatccgccg ccgccattca cgacctcgac cctgcagcag
     3001 gaggcctcgc gcaagcttgg gtttgcgcca gcaaggacga tgcagcttgc gcaaaggctc
     3061 tacgaaggcg ccgatatcgg cggagagacg gtgggtctca tcacttatat gcgtaccgac
     3121 ggcgtcgaca tcgcaccaga ggccatcgcc agcgcgcgca agctgatcgg cagccaatat
     3181 ggcgccgatc atgtccccgc agcgccgcgc aaatattcgg caaaaaccaa gaacgcgcag
     3241 gaagcgcatg aagcgatccg cccgactgac ctctcgcgac agccaagaca tgtcgccaaa
     3301 agcttgaagc cggatcaggc aagactttac gaactcattt ggacgcgaac gctcgcctgc
     3361 cagatggaat cggccgagct cgaacgcacg acggtcgata ttttggctga gacggaaagc
     3421 cgcaagcttg atctgcgggc gaccgggcaa gtgatccgtt tcgatggctt tctaaaactc
     3481 tatcaggaag gaagtgacga cgaggagggc gaggatggcg gccggctacc tgcaatggcc
     3541 gctggcgaag cgctcggacg agaaaacgtc gatataaaac agcattttac tgagcctccc
     3601 ccgcgcttta ccgaggccac cctaatcaag cggatggagg agcttggcat cggccggcct
     3661 tcaacctacg cctcgacctt ggctgttctg cgcgaccgga gttatgttcg cctcgaaaaa
     3721 aagcggctta ttccagagga caagggccgt ctcgtgacgg catttttgga aagctttttt
     3781 gcccgatatg tcagctatga ctttaccgca gcgctcgagg aagatctcga ccgcgtctcg
     3841 aaccatgagc taaactggaa ggacctgctg cggaactttt gggaagattt ttctgacgcg
     3901 ctcgcgggca tcaaagatct gcgcaccgca caagtcctcg acagtctgaa cgagcttttg
     3961 ggaccgcata tttttctccc aaaaggagac ggctccgatc cccgcgtctg cccgtcttgc
     4021 ggccttggcc agctgtcgct taagcttggc aaattcggcg ctttcattgg ctgttccaat
     4081 tatcccgaat gcaaattcac gcgcccgcta gtgaatgccg atggggatgg cgcttccaat
     4141 ggcgagcggt cgggggcaaa aatcttgggc gaagatccgg cgagtggaga agagatctct
     4201 ctgcgtcaag gccgctttgg cgcctatgtg caacagggcg aaggtgaaaa gccgaagcgg
     4261 gcgtccctgc caaaaaccat cgaagccgag gcactgacgc tcgcacaggc aattggcctt
     4321 ctttcgctgc cgcgcgaaat caccaaacat cccgagacaa aggaaccgat tgttgcgggt
     4381 atcggccgtt tcggtcccta tgttcagcat ggcaagacct atgcgaatat tggcaaggac
     4441 gacgacatat tgcgcatggg tgcaaatcgg gcgattgacc tcatcatcgc caaagagagc
     4501 ggcctgtcgg ggcggcgttt tggcgattcc gcgagcgccc cttcacggac actcggggag
     4561 catccgaagg gcggcgagct cgtcatcaag gccgggcgct atgggcccta tgtcaaatat
     4621 ggcaagatca acgcctcctt gccgcaaggc gccgacccca ccacttttcc cctcgaggag
     4681 gctgtggcac ttgtcgcggc caaggagg
//
LOCUS       DATMPU010000313         6391 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_3656518,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000313 DATMPU010000000
VERSION     DATMPU010000313.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 6391)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6391)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6391
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3656518"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            460..1290
                     /locus_tag="VNY06_07130"
     CDS             460..1290
                     /locus_tag="VNY06_07130"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003597705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, water dikinase regulatory protein"
                     /protein_id="HWX14608.1"
                     /translation="MRRQYFHLHLISDATGETLIAASRAAAAQYEGVTSIEHVYPLVR
                     SRAQLDKAIAEVEAAPGIVLFTLVDQELAKRLESACKELSVPCLAVLQPILNLFQSYI
                     GAASMHRPGAQHMLNSEYFKRIEALNFTMLHDDGQQVENFEAADVVLLGVSRTSKTPT
                     SIYLANRGIKTANIPIVPDIPLPDPILALRGPLVVGLIASVERIVQIRENRLLALKAR
                     PDSPYVDRFIVAGEVARARRLFAEHGWPIIDVTHRSIEETAAAVIDFYKIHQLKFIAS
                     "
     gene            1290..1901
                     /locus_tag="VNY06_07135"
     CDS             1290..1901
                     /locus_tag="VNY06_07135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012591529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase"
                     /protein_id="HWX14609.1"
                     /translation="MTALWRAGAPLVLASRSRARQDLLAAARIPFEARAAAIDERAIE
                     AELVASGAGASEVALHLSRAKASKVSAEMPGRLVIGADQVLCVEGQLFGKAADRAAAM
                     RQLQTLSGRTHELHSGCCAARDKRIVFEIVSVARLSCRQLSHDLIETYLFETGGLDSA
                     GTYRVEGLGIHLFDAIEGDHWTILGLPLIPLLKFLREEGSLLS"
     gene            1898..2752
                     /locus_tag="VNY06_07140"
     CDS             1898..2752
                     /locus_tag="VNY06_07140"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012591530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HWX14610.1"
                     /translation="MRSSLQDKPKACVIGWPVGHSRSPLIHGFWLNRLGIKGSYECAE
                     VSPGDFPGFMDNFARLGYAGGNVTLPHKQAAFERCARTTETAACLNAVNTLWIEAGKL
                     QGDNTDVAGFLAALDQEAPDWDLNSGHAVVIGAGGAARAIVYALQLRGLRRILLVNRT
                     KSRAATIAADIGQPIEVVDDSNLQRSLAGAALLVNATSLGMLGQPPLTIDLTPLPPEA
                     TVSDIVYVPLETALIKAAKSRGLLAVSGIGMLLHQAVPGFERWFGIRPSVTAELRGFI
                     EADISAAL"
     gene            2752..3348
                     /gene="coaE"
                     /locus_tag="VNY06_07145"
     CDS             2752..3348
                     /gene="coaE"
                     /locus_tag="VNY06_07145"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012113853.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HWX14611.1"
                     /translation="MFVLGLTGSIGMGKSTTAAMFRAEGIAVHDSDAVVHELYKGEAA
                     GAIESAFPGTTRSGVVDRALLANVVLEDSAALARLEAIIHPLVAASRDVFLVEAAKNG
                     ASLVVLDIPLLFETQSDRIVDAVVLVTAPESVQKARVCARPGMTAERFAAILPRQMPD
                     TEKRARAHFIIDTGRGFAAAERQVRGIVRALAGARNKV"
     gene            3357..4094
                     /gene="dnaQ"
                     /locus_tag="VNY06_07150"
     CDS             3357..4094
                     /gene="dnaQ"
                     /locus_tag="VNY06_07150"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012591532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HWX14612.1"
                     /translation="MREIVVDTETTGLDPENGHRIVEIGCVELLNTIPTGEIFHSYID
                     PERDMPESAFRVHGLSAEFLAGKPAFAKVASDFLTFIDGAKIIAHNAEFDMRFLNAEL
                     DLLGLGPIAHDRVVDTLSLARRKFPGAANSLDALCARFGIDTSRRTKHGALLDAELLA
                     EVYAELTGGRQAVLTFGLGEVSTIPNVRLLTQRPHPLPPRLSDSELSRHRSFVAGLGG
                     VPVWDRYLNLPDESAVHDRIKVSAQKS"
     gene            complement(4177..>6391)
                     /locus_tag="VNY06_07155"
     CDS             complement(4177..>6391)
                     /locus_tag="VNY06_07155"
                     /inference="COORDINATES: protein motif:HMM:NF019843.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX14613.1"
                     /translation="GFSHSSTFPSQGEDCGSQLTLLQQIEAAHTRLAEQLQTGFAAAA
                     TEIGALRTAVSSAIDKIAFTPGVDQSQQAPTWPEQPSAALVKRLDLAASGFESLTAIQ
                     NSISELFVQVEELRRAVTVISMPDEQAPQRLSPSDKTERVILDGIANLRALHEDTANR
                     ALLAWTSVQTSLEQVTGLCARLEVAATQERVERPSAGIDPNDPFVPLLAHLAQQGKNG
                     APQLSGTEGPRTGTDESAQDDAPHSLGDVDASGFLIEPGHGFPGLNENREPQKTGNPQ
                     PTIFLEREDSPSRTDFIAAARRAARTARLEAQGKAPVADAGETSGGKMLPSLLRRCKR
                     LFSTCRRAIVLATAILLAAIVTIAVIRLPILNKVSDFLPTFLRQFYSTGARAEKPPAL
                     DSVSKTAVIQTLPPAMFAELPSADGRSRLGPPQSASAATNTRVAALRPDPLAPPGGWA
                     SAAGAGDRASPASRAISGSDAIVTAAMLPSKEIAAQASGHDPLGTPPVRTLPSKAQPH
                     PPPSGNGPAIASAAADSLSDPAAQFELAAHYAEDTSSPGKLALAAQWYEKAAQQGHAV
                     AEYRLASLYEKGRGVAKDIERAKELYQRAAERGNIRAMHNLGVLAAEGSDGKPNYTSA
                     ALWFSKAAGYGIKDSQYNFAVLLARGLGVIKDLVRSYTWFAIIAATGDADAARKRDEV
                     AARLTSSELSAANAAAAAFVPDTPDRAANEPAPPPAGQDTAKANALPVKSKVSGL"
BASE COUNT         1270 a         1739 c         1920 g         1462 t
ORIGIN      
        1 gagaccttgc cagcaagagt ttccagaaac cggttggttt tcatgcgaga tttctctacc
       61 aaatccaaat ctctctaaaa accaataatc ttctttaaaa aagttgtttt tatctgttta
      121 aaacgcgggt tgagtgcgtt gaccaagatg cacaggctaa agggtgattt acggttttcc
      181 gactttttaa caagctatgc ctttcgttgt ggtcgcggga catattcgaa ttcgaaaaac
      241 cagttgtgtg ttgatttgga ctcgttggtc agagcttttg ccttttcaac tccattcaca
      301 ggcacaagac aaaatttgtg gcgcggctcg taccggtgaa agaatgcgga tatcttcttt
      361 aacaaaaggg ttgccagcag gcagccgctc cagctatccc cacctcttcc cggtgttgct
      421 ctgagttttg ggaaaaaccg tggaaagggg tgaaagctct tgaggcgtca gtactttcac
      481 cttcatctca tttcggatgc gacaggggag accctgattg cagcgagccg cgctgctgcg
      541 gcgcaatatg aaggtgtgac gtcgattgag catgtatacc cgcttgtgcg cagccgggcg
      601 cagcttgata aggctatagc cgaggtggaa gcagccccag ggatcgttct gttcactctg
      661 gtcgatcaag aattggccaa gcgcctcgaa tccgcctgca aggagctcag tgtcccctgc
      721 ctggcggtcc tgcagccaat cctgaacttg tttcagtcct atattggggc tgcatcaatg
      781 catcgccctg gggcgcagca catgctgaat tcggagtatt ttaagcgcat tgaggcgttg
      841 aattttacaa tgctccatga cgatgggcag caagttgaga attttgaagc ggccgatgtg
      901 gttttattag gcgtaagccg cacctcgaag acgccgacga gcatctatct tgccaatcgc
      961 ggtatcaaaa ccgcgaatat tccaatcgtg ccggatattc cgctcccaga cccaattttg
     1021 gctttgcgtg gccctcttgt tgtcggactg attgcttcag ttgagcgcat cgttcagatc
     1081 cgcgaaaatc gcctccttgc gttaaaagct cgcccggatt ccccctatgt ggatcggttt
     1141 atcgttgctg gggaggtcgc acgggcacgc aggctttttg ccgaacatgg ctggccaatc
     1201 attgatgtga cccatcgctc gattgaagaa acggcagctg cagtgatcga tttctacaag
     1261 atccatcagt tgaagttcat tgcctcatga tgacagcatt atggcgtgcc ggcgcacccc
     1321 ttgtccttgc ctcgcgaagc cgcgcccgcc aggacctact tgccgcagct cgcataccat
     1381 tcgaggctcg cgccgcggcg atcgatgaac gcgctatcga agccgagctc gtcgcttcgg
     1441 gcgcgggagc gagcgaagtc gctcttcatc tttcgcgggc aaaagcttca aaggttagcg
     1501 cggaaatgcc gggccgcttg gtcataggcg cagatcaagt gctttgcgtc gaaggacaac
     1561 tatttggcaa agcggctgat cgcgcggcgg cgatgcgcca gcttcaaacc ttgtcgggtc
     1621 ggacacatga gctgcattca ggctgttgcg ctgcgcgcga caaaaggatt gtctttgaaa
     1681 tcgtctctgt agcgcggcta agctgcaggc aattgtccca tgatttaatc gaaacctatc
     1741 tttttgaaac agggggcctc gatagcgctg gcacctaccg ggtcgagggt ctcggcatcc
     1801 atctcttcga tgcaatcgag ggggatcatt ggacgatcct gggccttccg ctcataccgc
     1861 tcctcaaatt cttgcgtgag gaaggaagcc ttctgtcgtg aggtcttcgc ttcaagacaa
     1921 accaaaagcc tgtgtgattg gctggccagt tggtcactca cgttcgccat tgatccatgg
     1981 attctggctc aatcggctcg ggattaaggg gtcctacgag tgcgccgagg tttcgccggg
     2041 agattttccg ggtttcatgg ataatttcgc gcggcttggc tatgcaggag ggaatgttac
     2101 cttgccgcat aaacaagctg ccttcgaacg ctgtgcgcga acgaccgaaa cagcggcctg
     2161 ccttaatgct gtgaatacat tgtggataga ggctggaaaa cttcaaggcg acaacaccga
     2221 tgtcgccggt tttcttgcgg ctctcgatca ggaagcgccc gattgggacc ttaattccgg
     2281 acatgccgta gttattggtg cgggaggcgc cgcgcgggcc atcgtctatg ccctgcagct
     2341 gcgcggccta aggcgcatct tgctcgtcaa tcgcacgaaa tcgcgcgcgg cgacgattgc
     2401 agcggatatt ggacagccca tcgaagttgt ggatgattct aacttgcagc gatctctggc
     2461 cggagctgcg ctgctggtca acgccacttc gcttggcatg ctgggccagc cgccgctcac
     2521 aattgatcta acgcctctgc caccagaggc cacggtcagc gatatcgtct atgttccgct
     2581 ggaaacggct ttgatcaaag ctgcgaaatc gcgtggcctc ctcgcggtct ccggtattgg
     2641 catgctgctg caccaagcgg tcccagggtt tgagcgctgg tttggcatca gacccagcgt
     2701 cacggcggag ttgcggggtt ttattgaggc ggatatttcg gcggcgctct gatgttcgtt
     2761 ctcggcctca cgggctcgat cggtatggga aaatcgacca ccgcggcgat gtttcgcgcc
     2821 gaaggaatag ccgtccatga ttccgacgcc gttgttcacg agctttataa gggggaagcg
     2881 gctggcgcca ttgaatcggc ctttccagga acgacgcgaa gtggcgtggt tgatcgcgcg
     2941 ctgctcgcca atgttgtgct cgaagattcg gcggcattgg cgcggcttga agcgatcatt
     3001 cacccgctcg ttgccgcaag ccgggatgtt ttcctcgttg aggcggcgaa aaatggagcg
     3061 agcctcgttg tgctcgatat ccctttgctt ttcgagacgc aaagtgaccg cattgtcgat
     3121 gcggttgttc tcgtcaccgc tcccgaatct gtgcaaaagg cgcgcgtatg cgctcgcccg
     3181 ggcatgacgg cggagcgatt tgccgccata ttgccgagac aaatgcccga tacggaaaaa
     3241 cgcgcgcgcg cgcatttcat tatcgatacg gggcgcggat ttgccgcggc ggagcggcaa
     3301 gtgcgcggga ttgttcgggc gctggctggc gcgagaaaca aggtttgaga gcaaaaatgc
     3361 gcgagattgt cgtcgataca gaaacgacgg ggctcgatcc ggagaatgga cacagaatcg
     3421 tcgagatcgg ttgcgttgaa cttttgaata cgattccgac cggcgagatt ttccatagct
     3481 atatcgatcc ggagcgcgac atgcccgaat ccgcgttccg ggtgcatggg ctgtcggccg
     3541 agtttctcgc tggcaagccg gctttcgcca aggttgctag cgattttctg acattcatcg
     3601 atggtgcgaa gatcattgcg cataatgctg aattcgacat gcggtttctg aacgcggaac
     3661 tcgatctcct cggacttggg ccgatcgctc acgaccgcgt cgtcgatacg ctttccttgg
     3721 cgcggcgtaa atttccgggc gccgcaaaca gcctcgacgc gctttgtgcg cgctttggta
     3781 tcgatacatc acggcgcacc aaacatggcg cgttgctgga tgccgaactt ctggccgaag
     3841 tttatgccga gctaaccggc gggcggcagg cggtgctgac cttcggcctt ggggaggtct
     3901 caaccattcc gaacgtgcgg ctgctcacac aaagacctca tccgcttccg cctcggctga
     3961 gcgacagcga actctcccgg caccgcagtt ttgtcgccgg actaggcggg gtgcccgttt
     4021 gggatcgcta tttaaatctc cccgatgaga gcgcagtgca cgatcgcata aaggtgtctg
     4081 cacagaaaag ctgacgcaat aggcgtggtg atctggagca agagggcgat acgaaaaaaa
     4141 ctcctctagc tttagctggc accatgacga acaatttcaa aggccagaaa cctttgactt
     4201 gacaggaagg gcgtttgctt tggcggtgtc ctgtccggca gggggtggcg cgggttcgtt
     4261 cgccgcgcgg tccggagtgt ctgggacaaa ggcggcagcg gccgcattgg ctgcggagag
     4321 ctcggaactg gtgagtctag ccgcaacctc atcgcgtttg cgggcggcat ccgcgtctcc
     4381 cgtggcggcg atgatcgcaa accaggtgta agaccgcacg aggtctttta tgaccccgag
     4441 cccccgtgca agcaaaaccg cgaaattgta ctggctgtcc ttaatcccat atccagccgc
     4501 tttgctgaac cacagtgcgg cgctggtata attgggtttg ccgtcacttc cttcggccgc
     4561 gaggacaccg aggttatgca tggcccgaat attgcctctt tccgccgccc gctgatagag
     4621 ttccttggcg cgctcaatat ccttggcgac accgcgccct ttttcataga gggatgcaag
     4681 ccggtactcc gccaccgcgt gaccttgttg cgctgctttc tcataccatt gcgctgccag
     4741 ggcgagcttt cctggggagg aggtgtcttc ggcataatgg gctgcaagtt cgaattgagc
     4801 cgccgggtca gaaaggctat ctgctgcagc tgaggcaatc gccgggccat tgccggaagg
     4861 cggcggatgc ggctgcgcct ttgaagggag agtgcggacc ggcggcgtgc caagtggatc
     4921 atgcccggac gcctgagcgg ctatttcttt gctgggcagc atcgccgcgg tgacgatggc
     4981 atcactccct gagatggcgc gactcgcagg cgaagctcta tctcctgccc ccgcggcaga
     5041 cgcccaacct cccggcggtg ccaaaggatc gggtcgaagg gcggcgacgc gtgtgttggt
     5101 tgctgcggag gcactttgcg gcgggcccag tcggctacgg ccgtcagctg atggcagctc
     5161 cgcaaacatt gccggaggca aagtctgaat tacagctgtc ttactaacag aatcgagagc
     5221 agggggcttt tcagcgcgcg cgcccgtgct gtagaactgc ctcagaaatg tgggcagaaa
     5281 gtcgctcact ttgttgagga tcggtagcct gataacagca atcgtaacaa tggcggcgag
     5341 caatatggca gtggcaagaa cgattgcgcg ccgacaggtc gagaaaagtc ttttgcaacg
     5401 acggagaagc gaggggagca ttttccctcc gcttgtttcg cccgcatcag caaccggcgc
     5461 tttcccttgc gcttccaacc gagcagtccg cgccgcacgc cgggcggccg cgatgaagtc
     5521 cgtacggctt gggctgtctt cgcgctcgag aaagatcgtg ggctgtggat tgcccgtctt
     5581 ctgcggctcg cgattttcgt tgaggccagg aaagccgtgg ccgggttcaa tcagaaatcc
     5641 ggatgcgtca acgtcgccca aactatgcgg ggcatcgtct tgggcggatt cgtcggtccc
     5701 agtccttggg ccttcagtcc cgctcagctg gggagcgccg tttttacctt gttgtgcgag
     5761 gtgagcaagg agcgggacaa agggatcgtt agggtcgatt ccggcgctgg gacgctctac
     5821 gcgctcctgt gtcgcggcga cttcgagacg tgcacagagc ccggtcactt gctccaatga
     5881 cgtttggact gacgtccagg cgagcagggc gcggtttgcc gtgtcctcgt ggagggcacg
     5941 taaatttgca atcccgtcca aaatgactcg ctccgtcttg tcggatggcg aaagcctctg
     6001 cggggcttgc tcgtctggca tcgagatcac ggtaaccgcc cggcgtaatt cttcgacttg
     6061 aacaaagagc tcgctgatgg aattttggat cgcggtcaaa gattcaaaac cgctcgccgc
     6121 aagatcgagc cgtttaacaa gtgcagcgga aggctgttcc ggccaagtag gcgcttgctg
     6181 gctctgatcc acgccgggcg taaacgcaat tttgtcgata gcgctcgata cggcggttct
     6241 caacgcgcct atttcggttg cagcggcagc aaatcctgtt tggagctgtt cggcaagacg
     6301 cgtgtgtgct gcctctattt gttgcaaaag cgtgagctgg ctgccgcagt cctcgccttg
     6361 cgacgggaat gttgaagaat gggagaaccc g
//
LOCUS       DATMPU010000314        10531 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_4252356,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000314 DATMPU010000000
VERSION     DATMPU010000314.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 10531)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10531)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10531
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4252356"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(290..1192)
                     /gene="truB"
                     /locus_tag="VNY06_07160"
     CDS             complement(290..1192)
                     /gene="truB"
                     /locus_tag="VNY06_07160"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012320659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HWX14614.1"
                     /translation="MMVRAGRRNNVHGWLVLDKPAGMTSTHAVSRLKRLFNAKKAGHA
                     GTLDPIATGVLPVAFGEATKTVPFVQEGEKAYRFTVRWGIETNTDDCEGEAVTQSEIR
                     PTAAQIEALLPRFTGLLEQRPPAFSAIKINGERAYDLARGGEVPELSARTVTIHAFDL
                     INAGREAAIFEARCGKGTYVRALARDLGRSLGCYGHVSALRRTRVGPFFEVDAIGLEE
                     LEKDPSGAQALRHVEAGLSELRRVAVDKGVAAKLRRGQPVLLRGQDLPLSGLAYAACG
                     DVVIAVGAIEKGELIPGRIFNLSP"
     gene            complement(1189..1602)
                     /gene="rbfA"
                     /locus_tag="VNY06_07165"
     CDS             complement(1189..1602)
                     /gene="rbfA"
                     /locus_tag="VNY06_07165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012591294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HWX14615.1"
                     /translation="MSRSHHQAGGEPSQRMLRVAELIRQAMSDLLVRTALGDPVLESR
                     TITIPEVRVSADLKHATIYVMPLGGQGIPEVLAALGRQRKHLRAEIAHRINLKFAPDI
                     RFQADPRFDYDAKINALLALPQVKRDLDKDRDKQE"
     gene            complement(1708..4326)
                     /gene="infB"
                     /locus_tag="VNY06_07170"
     CDS             complement(1708..4326)
                     /gene="infB"
                     /locus_tag="VNY06_07170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012384047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HWX14616.1"
                     /translation="MTETKNPGDTLSVGSAKKLTLKRPVEAGIVRQSFSHGRSKAVVV
                     EKVKRRSIGPGEVHAPREANVSPPAPAAPPRPRPQSVPAHPQAPAQRSPSGVILRTLT
                     DEEREARSRALSDAREREAEQRIRAEIEAKAREGREAREREERAAAEERKREEESRRA
                     HDAEAKRRSETEAKRRLAGGEPAPVPVGLRKPPPATKPQGSVGLSPLPRPVLTEEEEA
                     PHSGRRPGVVTKIAVPRPARGAEPKNRGRLTLSTVTGEEEDERTRSVAAFRRRVQRLK
                     AHITEAKEKIAREVILPETITIQELASRMSERAVDVIKLLMKQGQMAKITDVIDADTA
                     QLIAEDLGHTVKRVAEADVEEGLFDTPDSDRDLLSRAPVVTIMGHVDHGKTSLLDAIR
                     HANVVSGEAGGITQHIGAYQIIAPNGSKVTFIDTPGHAAFTAMRARGAKVTDIVVLVV
                     AADDGVMPQTAEAIAHARAANVPMIVAINKIDKPDAKPERVRNELLQYEVQVESLGGE
                     TLEAEVSATKKINLDKLLELIALQAELLDLRANPARPAEGTVIEARLDKGRGPVATVL
                     VQRGTLKVGDLIVAGSEWGRVRALLDDTGTARHDAGPSTPVEVLGFSGSPEAGDRVAV
                     VETETRAREITEYRERQRREKAAARGTVARGSLSDMMSQLKTAGRKEFPLVIKGDVQG
                     SVEAIVATLEKLNTEEVAARVIHAGAGGITESDVTLAEASRAAVIGFNVRAHKEARAL
                     ADQTGIEIRYYNVIYNLIDDVKAAMSGLLAPSLREEMLGSAETLEIFTISKVGKVAGC
                     RVVEGNVQRGAQVRLIRDNVVVHEGKLSTLKRFKDEVKEVLAGQECGMAFENYQDMRV
                     GDIIECYTVQEIKRSL"
     gene            complement(4347..5051)
                     /locus_tag="VNY06_07175"
     CDS             complement(4347..5051)
                     /locus_tag="VNY06_07175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012384048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HWX14617.1"
                     /translation="MARPECCSAAGSPERTCIVTRAKALPRDMIRFVVGPGAAVVPDI
                     RRKLPGRGVWVLGRADIVAKAVKGQVFSRGFKKKVTASHDLAVEIEKELTRDCLQALS
                     IANKAGQVVTGFAKVEEAIASGTVCGLVHAAGCSADGVRKLGRCMRWRFGDNRAKPYI
                     SLFRSDELDLALGRTNVIHAALAEGAASEGFLDRCRRLRLYRSASSRAEGRDTAVWPT
                     QSEESNGLRALGHAAT"
     gene            complement(5063..6658)
                     /gene="nusA"
                     /locus_tag="VNY06_07180"
     CDS             complement(5063..6658)
                     /gene="nusA"
                     /locus_tag="VNY06_07180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020174352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HWX14618.1"
                     /translation="MAVSANRLELLQIADSVAREKSIDRQIVLSAMEDAIQKAARARY
                     GQETEVRADINPKTGEIRFSRLLLVVDQIDNDANQITVSEARRKNPAAQPGDWIAEPL
                     PPFEFGRIAAQSAKQVIVQKVREAERDRQYEEYKDRIGDIVNGVVKRVEYGNVIIDLG
                     RGEATIRRDELIPREVFRPGDRVRAYVYDVRREQRGPQIFLSRTHPQFMAKLFRQEVP
                     EIYDNVIEVKSVARDPGSRAKIAVLSRDSSIDPVGACVGMRGSRVQAVVNELQGEKID
                     IIPWSADAATFIVNALQPAEVVKVVLDEDSARIEVVVPDDQLSLAIGRRGQNVRLASQ
                     LTGWDIDILTEAEESERRQKEFAERTSTFMKALNVDEVVGQLLASEGFRSVEELAFVE
                     PSEVAAIEGFDQDTAAEIQSRAHEYLAKLESEQDVRRRELGVSDELKEVAGVTMAMLV
                     KFGENGIKTVEDLAGCATDDLIGWTERKEGETKKHAGILDGFEISREEAEGLIMTARL
                     RAGWIDSIPGPTEKDLFANEIGN"
     gene            complement(6639..7445)
                     /gene="rimP"
                     /locus_tag="VNY06_07185"
     CDS             complement(6639..7445)
                     /gene="rimP"
                     /locus_tag="VNY06_07185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020187220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HWX14619.1"
                     /translation="MSSATLPEAQSALDEPRLVEEIGVAARIAHIAQPVLASHGYRLV
                     RVKLSAQAGMTVQIMAERPDGSMTVDDCEIVSGALSPVLDVEDLVKSAYRLEVSSPGI
                     DRPLVRISDFRQAVAQEARIEMRTAVGGRRRFPGRIIAVEEDGECAKLILERSDAKPG
                     EATEAALSLHDIAEAKLVLNEALLRQSLRAAKALWADVRPGEAEPSMDTPDRGMGRGP
                     RRGVEARAALPGGAGPALKESRSKRAKSSTPQRSGAGSPETGDRDGSKRE"
     gene            7977..8063
                     /locus_tag="VNY06_07190"
                     /pseudo
     CDS             7977..8063
                     /locus_tag="VNY06_07190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002971893.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
     gene            8200..9468
                     /gene="lysA"
                     /locus_tag="VNY06_07195"
     CDS             8200..9468
                     /gene="lysA"
                     /locus_tag="VNY06_07195"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018406291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HWX14620.1"
                     /translation="MHHFAMQNGVMHAEDVELPTLANEIGTPFYCYSTATLKRHFDTF
                     DSALCNLPRLICYAVKANSNQAVLRSLARLGAGMDVVSGGELRRALAAGVSPEKITFS
                     GVGKTGEEMAFALKLGILGFNVESEPELALLSELAQSSSRPAHIALRVNPDIDANTHA
                     KISTGKAENKFGIPIGRAREVYAHAAMLPGIAIRGIDIHIGSQITDLAPFDAAFALIA
                     EFVRVLRADGHEIEHLDLGGGLGIPYAHWEDPESYHPERYADIVKTRLGSLNCRLIFE
                     PGRLIAGNAGILVTSVLCVKKGEAKKFIVVDAAMNDLVRPTLYDAYHEILPVQPRKGN
                     SEFIADIVGPVCETGDYFALSRKIAEPLPGDLLAIMSAGAYGAVQAGTYNSRLLVPEV
                     LVNGSQYCIVRPRGTYEELIGLDKIPAWLT"
     gene            9598..>10531
                     /locus_tag="VNY06_07200"
     CDS             9598..>10531
                     /locus_tag="VNY06_07200"
                     /inference="COORDINATES: protein motif:HMM:NF025152.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4175 family protein"
                     /protein_id="HWX14621.1"
                     /translation="MRPVCPQRSDAAINPRLEWLVAKVQAILLFERIWRAILPPLVVT
                     GAFVSISWTGVWLDAPHWARGLGILVLALGLTAALLRLRGFRWPSRKEALQRIDQTSG
                     LSLRPAAVIADRLGNGVNNPVTVALWNLHRRRAEQAMLWLRTGGPSPRAAELDRYALR
                     AIVIVALIATGFVAGPERYARIAAAFDWSFTPISRTESRVDAWIDPPAYTGKMPIVLN
                     LARSRSFVMPEGPQRIEAPARSIVVVHAPEGKLDIEVKGGLTRSVTDSITAAKANGAP
                     PAKPADGTREGQIETRLVLRGDATLTIRSSGTHLG"
BASE COUNT         2033 a         3319 c         2872 g         2307 t
ORIGIN      
        1 ttgtggcaat tatggccttc gagtctgatt atacgcacgc tactcggatc acatttttcg
       61 gaatggcagg ctccagctcc agtacaccgg ggaccaatac ttaccacaag tctgccgcgt
      121 ccaatctttc agaggcggag aagtagtgta ggaacctcat agattggata gtacgctggc
      181 ttgttttctg acagtggcct caatccagaa gcccaccgac ccccctcgcc cgcgtccatg
      241 ttacttttgc cgaggatcct gcagagcgac ctaacacccc gcgagatggc tacggagaaa
      301 ggttaaagat gcggccagga atgagctcac ccttttcgat cgcgccaacg gcaatgacaa
      361 cgtcgccaca agccgcgtaa gcgagcccac taagcggcag gtcttgaccc cgcagcaaaa
      421 ccggctggcc gcggcgaagt tttgccgcaa cgcccttatc gacagccaca cgccgcaact
      481 cagaaaggcc ggcctcgaca tggcgcaggg cttgggcgcc cgatgggtcc ttttctaatt
      541 cctccaaccc gattgcatct acctcaaaaa acggtccgac gcgggtccgc cgcaaggcgc
      601 tgacatgtcc atagcagcca aggcttcggc ccagatcgcg ggcaagcgcg cgcacatagg
      661 tgcccttgcc gcagcgggcc tcaaaaatcg cggcctcacg ccccgcgttg atgagatcga
      721 atgcatgaat tgtaacggtg cgagcagaca attccggcac ctcgccgcca cgcgccaaat
      781 cataggcgcg ttcgccattg atcttgatcg cggaaaaggc gggcggtcgc tgctcgagca
      841 gcccggtaaa tcgcggcagc agcgcttcga tctgagccgc cgtgggacgt atctcggatt
      901 gcgtgacagc ctccccctcg caatcgtcgg tgttggtttc aatcccccag cgcactgtga
      961 agcgataagc cttttcgcct tcttgaacaa aaggcacggt ctttgttgcc tcaccaaaag
     1021 caaccggcaa gactcccgtg gcgatcgggt caagggtacc tgcgtggcca gccttctttg
     1081 cgttgaacag gcgcttcaac cgggacacgg catgcgttga cgtcataccg gctggtttat
     1141 cgagcacgag ccagccgtga acgttgtttc gccttccggc gcggaccatc attcttgctt
     1201 gtccctatct ttgtctaagt cgcgtttcac ttgcggcaat gcgagcaatg cattgatctt
     1261 cgcgtcataa tcgaagcgcg gatctgcctg aaaacggata tccggcgcga attttaggtt
     1321 gattcgatgc gcgatttccg cacgcaggtg tttcctctgc cggcctagcg ctgcgagaac
     1381 ttcagggatg ccctggccgc cgagcggcat gacatagatt gtggcgtgct tcaaatccgc
     1441 gcttacgcgt acttccggaa tcgttatcgt ccggctttcg agaaccggat cccccaaagc
     1501 ggtccgaacc aataggtcgg acatggcttg acggatgagt tcggccacgc gaagcatccg
     1561 ctgcgaaggc tcgccccccg cctggtggtg cgatcttgac atgataactt tggcgtatga
     1621 tcccgttcgg gaacgtggtt ggtttgcact cctgcgcggt tcgcttttcg caccccgccc
     1681 gcctgtaaga atgcatagtc tttcgcgtta caacgagcgc ttgatctcct gcacggtata
     1741 gcattcgatg atatcaccta cccgcatgtc ctggtaattc tcgaatgcca tgccacattc
     1801 ttgacccgcc aagacttctt tgacctcgtc cttgaagcgc ttgagcgtcg aaagcttacc
     1861 ttcgtgcacc accacattgt cgcggatgag cctgacttgg gcgccacgct gaacgttgcc
     1921 ctctacaaca cggcagcccg cgaccttgcc cacctttgaa atcgtgaaga tctccaatgt
     1981 ctcggcactg ccgagcattt cctcgcgcaa gctcggagca agcagacccg acatcgcggc
     2041 cttgacgtca tcgatgagat tgtagatgac gttgtaatag cgaatctcaa tgccagtctg
     2101 gtcggcaagc gcgcgggctt ccttgtgggc acgcacatta aagccaatca ctgctgcccg
     2161 cgaggcttcg gccaatgtta cgtcagattc ggttatgccc ccggcacccg catgaataac
     2221 acgtgccgcg acctcctcgg tattgagttt ttcgagcgtc gcgacgatgg cctccaccga
     2281 cccctgaaca tcgcccttga tgacgagggg aaattccttg cggccagcgg tcttcagctg
     2341 gctcatcata tcggagagcg aaccgcgtgc gacggtaccc cgtgcagcag ccttttcgcg
     2401 ccgttgccgt tcgcgatatt cggttatctc ccgcgccctc gtttccgttt caacaacagc
     2461 gacccggtcc cccgcttcgg gcgacccgga aaagccaagc acctccactg gcgtcgaagg
     2521 gccggcgtcg tgcctcgccg tgcctgtatc gtcgagtagg gcgcgcacac ggccccattc
     2581 ggagccggcg acgatcaaat cgcctacttt gagcgtgccg cgctgaacga gcacggtcgc
     2641 cactggcccg cggcccttgt cgagccttgc ctcaatcacg gtgccttccg ccggacgcgc
     2701 tggattggct cgcaggtcga gtagttcagc ttgcaaggcg atcagttcga gcagcttgtc
     2761 gagattgatt ttcttcgtcg ccgagacttc tgcctcgagc gtctcgccgc caagggattc
     2821 gacttgcacc tcatactgta gcaattcgtt gcgcacccgc tcaggcttgg catctggctt
     2881 atcgatcttg ttgatcgcca caatcatcgg gacattggca gcgcgcgcat gcgcgattgc
     2941 ttccgccgtc tggggcatga ctccatcatc ggcggcgaca acgagaacta cgatgtccgt
     3001 tactttggcg ccgcgggccc gcatcgcggt gaaagccgcg tggccaggcg tatcgatgaa
     3061 cgtcaccttg gagccattcg gggcaatgat ctgataggcg ccgatgtgtt gtgtgatccc
     3121 gccggcctca ccagaaacga cattggcatg acggatggca tccaagagcg aagtcttacc
     3181 gtgatccacg tggcccatta tcgtcacgac cggggcacgc gacaggagat ctcgatcgct
     3241 atctggcgtg tcgaagagcc cctcttcaac gtccgcttcg gcgacgcgct tgacggtgtg
     3301 accgaggtct tcggcgataa gttgcgcggt gtcggcgtcg attacgtcgg tgatcttcgc
     3361 catttggccc tgcttcatga gtagcttaat gacgtccacg gctcgctcag acatgcggct
     3421 ggcaagctct tggatcgtta ttgtttccgg caggatcacc tcgcgcgcga tcttttcctt
     3481 tgcctcagtg atatgcgcct tgagcctctg aacgcggcgc cgaaaagcgg ctacggatcg
     3541 cgtacgctcg tcttcttcct cgcccgtgac ggtggagagg gtcagacggc cacggttttt
     3601 tggctcggcg ccccgcgctg gacgtgggac ggcaattttc gtcaccaccc caggacgacg
     3661 gccagaatgc ggggcttctt cttcttcggt gaggaccggc cttggcaatg gcgaaaggcc
     3721 gacggaacct tgtggcttgg tggcgggcgg cggcttgcgc agaccgacgg gcaccggtgc
     3781 cggttccccg ccggcaagac gccgctttgc ttccgtttcc gagcggcgct ttgcctccgc
     3841 atcatgcgca cgccgcgatt cttcctcccg cttgcgctct tcagctgccg cacgctcctc
     3901 ccgttcccgg gcctctcgcc cttcccgggc ctttgcctcg atctcggcac ggatgcgctg
     3961 ttccgcttcg cgctcgcggg catcggaaag agcgcgagag cgcgcctcgc gctcctcgtc
     4021 cgttagcgtg cgcaggatta ctcccgatgg cgaccgctga gccggcgcct gcgggtgggc
     4081 gggaaccgat tgcgggcgcg ggcgcggtgg agcggccgga gcaggcggcg acacattcgc
     4141 ctcacggggc gcgtgcacct caccgggacc gatagaacgg cgttttacct tttcaacaac
     4201 aaccgctttc gagcgcccgt gcgaaaaact ttgccgcacg attccagcct cgaccgggcg
     4261 cttcaaggtc agcttcttgg ctgaaccgac gctcagcgtg tcacccgggt tcttcgtttc
     4321 cgtcatccgt cttgcgttcc cacccattag gttgccgcgt ggcctaaggc ccgtaggccg
     4381 ttcgattcct cgctttgcgt cggccagacg gccgtgtctc ggccttctgc ccgagacgaa
     4441 gccgaccgat aaagcctcaa gcgccggcac cgatccaaaa agccttcgct cgccgctccc
     4501 tcggcaagag ctgcgtgtat cacatttgtc cgtcccaatg ccaaatccaa ttcgtcggac
     4561 cggaacaggc tgatgtatgg cttcgcccgg ttatcgccaa aacgccaacg catgcaccgt
     4621 ccgagtttac gaaccccgtc agcgctgcag ccagcggcgt gtacaagacc gcaaaccgtg
     4681 cccgatgcaa tcgcctcttc gaccttggca aaacctgtga cgacttgacc cgccttattg
     4741 gcaatcgaca atgcctgcaa acagtctctg gtcagttctt tctcaatctc aactgctaaa
     4801 tcgtgcgacg ccgtaacctt ctttttaaat ccacgtgaga aaacctggcc cttgacggct
     4861 tttgcgacaa tatcggcgcg gcccagcacc caaacccctc gtcccggtaa tttcctccta
     4921 atgtcaggca cgaccgctgc gccgggaccc acgacgaaac ggatcatgtc tcgcggcaac
     4981 gctttagccc gagtgactat acaggtccgt tcgggcgaac ccgcagcgct gcagcattct
     5041 ggcctcgcca cgcgtttttc tcctaattgc cgatctcatt tgcaaataaa tctttttcgg
     5101 tcgggcccgg aatggaatcg atccaccccg ccctcagccg tgcggtcatg atgagaccct
     5161 cagcttcctc acgcgaaatt tcaaagccgt cgagaatgcc cgcatgtttt ttcgtctcgc
     5221 cctcttttcg ttccgtccag ccgatgaggt catcagtcgc acatccagcc aaatcctcaa
     5281 cggttttgat tccgttttcg ccaaatttga caagcattgc catcgttacc ccggcgactt
     5341 ccttaagttc gtcggataca ccaagttcac gccggcggac gtcctgttca gattcaagct
     5401 tggccagata ttcatgcgca cgagactgaa tctctgcagc cgtatcctgg tcgaagcctt
     5461 caatcgcggc aacttcggaa ggttctacga aagccaattc ctcgacagac ctgaatcctt
     5521 ccgatgccag caactggcca accacttcgt cgacgttcag cgctttcatg aaagtgctgg
     5581 tgcgctccgc gaattccttc tgccgccgct ccgactcctc cgcctccgtt aaaatatcga
     5641 tatcccagcc ggttagctgc gaggccaagc gtacattctg tccacggcgc ccgatcgcaa
     5701 gggaaagctg gtcgtcgggc acaacgactt cgatccgcgc agaatcctcg tcgagcacaa
     5761 ccttgacgac ttcggcaggc tgcaaggcat tcacgatgaa ggtggcggcg tccgctgacc
     5821 aagggatgat atcgatcttc tcaccctgga gttcattgac gacagcctgc acccgcgacc
     5881 cgcgcatgcc gacacaggcg ccaaccgggt cgattgagct atcgcgcgac aacaccgcga
     5941 tcttggcacg cgaccccggg tcgcgcgcga ccgacttcac ctcgatgacg ttgtcgtaga
     6001 tttccggcac ttcttggcgg aacaatttcg ccatgaattg aggatgtgtg cgtgaaagaa
     6061 aaatctgtgg cccgcgctgc tcgcgtcgaa cgtcgtagac ataagcgcga acacgatcgc
     6121 cgggccggaa cacttcgcgc gggatcaact cgtcgcgtct gattgtcgcc tcgccgcgcc
     6181 cgagatcaat gataacattg ccatactcta cgcgtttcac gacgccgttc acgatatcgc
     6241 caattctatc cttatattcc tcatactgtc tgtcgcgctc ggcctcgcga accttctgaa
     6301 caatcacctg cttggcggat tgcgcggcaa ttcgtccgaa ttcaaatggc ggcaggggtt
     6361 ccgcgatcca atcgcccggc tgtgccgcgg ggttcttcct acgcgcctcg ctaaccgtga
     6421 tctgattggc atcgttgtcg atctggtcga cgacgagaag cagccgcgag aatcggatct
     6481 ccccggtctt gggattaatg tcagcacgga cctctgtctc ttggccatac cgcgcccgcg
     6541 ccgccttctg aatagcatcc tccatcgcgg aaagaacaat ttgcctgtcg atcgattttt
     6601 cccgcgcaac ggagtcagcg atctgcaaaa gctccagcct attcgcgctt actgccatct
     6661 ctatctcctg tttcgggcga gcccgcgcca gatctttgcg gtgttgagga cttggctcgt
     6721 ttcgatctgc tttcctttaa cgccggtccg gcgccgcctg gaagcgcggc ccttgcctca
     6781 accccgcgtc gcggtcctcg gcccatgccc ctatctggcg tatccatcga aggctccgct
     6841 tcgccaggac gaacgtcggc ccagagcgct ttcgccgccc gcaacgattg gcgcaaaagt
     6901 gcttcattta ataccagttt tgcctcggcg atgtcgtgaa gtgaaagtgc agcttccgtg
     6961 gcttcgcctg gcttggcatc gctgcgctca aggatgagtt tggcgcattc cccgtcttct
     7021 tcgaccgcaa tgatccggcc cggaaagcgt ctgcgcccgc caacggcggt tctcatttcg
     7081 attcgcgctt cctgcgcaac ggcttgacgg aaatccgaaa tacgaacgag cgggcggtct
     7141 atgccaggcg aggagacctc gaggcgataa gcgctcttta ccaagtcctc aacatcgagg
     7201 accggtgaga gcgctccgct cacgatctcg caatcatcga cggtcatcga cccgtctggc
     7261 cgttcagcca tgatttgcac cgtcattccc gcctgtgccg aaagcttcac ccggacgagc
     7321 cgatatccat gactcgcgag aacgggttgc gcaatatggg caatgcgtgc agcgacgccg
     7381 atttcctcga ccaagcgagg ctcatccaac gcgctttgcg cttctggcag cgtagcgcta
     7441 ctcacgcgat ccagattccc cttattcgtc gaaggtcccc tccggcacta accaattcac
     7501 aatccgcttc gacaatgaca gtcccgaagg ggcaataaaa aagagcgggt cccggcgggg
     7561 gcccactctc aagcgatcgc ctcgatcgaa caaccgtcac gagaagcata aaccaagatc
     7621 tatatagagc aaggaagttg cctgtgcaac accgaatatc actctaatat cagccgcccc
     7681 atgcccggca gtcctagggc ttggcccctc ttaaatcgcg gtttagttat aagcgtgctt
     7741 cggaacagca aatcgcgtag gtccaagcga agactcgatt gctggttgac ggaatctttg
     7801 ggagctcgca ggccgtcatg tcttcagtcg tgagctgtcc caaaccgctg tccggccagc
     7861 atcttcaagt caaagctatc tgcgtcaggc aacgtgttca ttcctacgcc cttcgtttat
     7921 aatggctctc cttctggagc ttgctcgtgt cgcttagtcc ccccaaaatt gctttcatgc
     7981 tgtttttggc cgcgctagca ccttcgcttg cgtcatgcgg gaggcgcggc ccgctcgaac
     8041 cgccgccggg tgcgtccgta acgtgcgcgc ccgttacggg tacgcccggg gactatcaag
     8101 ccgcgggcga acctcagtct tcagccgcca agacatgtgc caaacagact tcgccaagac
     8161 cgttttttct cgatccccta ctctgacttt gagtgaccca tgcaccactt cgccatgcag
     8221 aacggcgtca tgcatgccga ggatgtggaa cttcctacgc tcgcaaatga gatcggtacg
     8281 ccattttatt gctattcgac ggctactctt aagcgccatt tcgatacctt cgattcggct
     8341 ctctgcaacc tgccccgcct catttgctac gcggtgaaag ccaattccaa ccaggccgtt
     8401 ctcaggtcgc tggcgcggct tggggcgggt atggatgtgg tctcgggagg cgaactcagg
     8461 cgcgcgctcg ccgcgggtgt ctcgccggaa aaaattacct tttccggcgt cggcaagacc
     8521 ggagaggaaa tggcctttgc gctcaaactt ggaattcttg gcttcaatgt cgaatctgag
     8581 ccagaacttg cgctgctatc cgaactcgca cagagctcca gccggccagc gcacatcgcg
     8641 cttcgggtta atcccgatat cgatgccaac acccatgcca agatctcaac cggaaaggca
     8701 gagaataaat tcggcatccc aattggtcgc gcccgcgaag tctatgcaca tgcggcaatg
     8761 ctccctggca tagcgataag gggtatcgac atccacatcg gctcgcaaat taccgacctc
     8821 gctccattcg atgcagcctt cgctctgatc gccgaatttg ttcgtgtcct gcgagccgat
     8881 ggacatgaaa tcgaacatct cgacctcggt gggggcctcg ggatcccgta cgcgcattgg
     8941 gaggatcctg agagttatca cccagagcgt tatgccgata tcgtcaagac acggcttggg
     9001 tccctcaact gccggctgat cttcgagccg ggccggctga tcgccggcaa tgccggcatt
     9061 ctcgttacaa gcgtcctgtg cgtgaagaag ggcgaggcaa aaaaattcat cgttgtcgac
     9121 gcggccatga acgatttggt tcgcccaacg ctttacgacg cctatcacga aatcttgccc
     9181 gttcagccgc gcaaaggaaa ctcagaattt atcgccgata tcgtcgggcc ggtctgtgaa
     9241 acaggcgatt atttcgcgct atcacggaaa attgccgagc ccttgccagg agacctcttg
     9301 gcgattatgt cggctggcgc ctacggcgcg gtgcaggcag gaacatataa ttcccgcctc
     9361 cttgtgccag aagtactcgt caatggcagc caatattgta tcgtccggcc gcgtggaact
     9421 tatgaggaat tgattggtct ggacaaaatt ccggcttggt taacttaaat atttggatct
     9481 cgcctgggct ttcggccctg gcgctgtgca gtgacctcga gtgagccttg cgcgaaagct
     9541 cgtcccgtaa gctttgcttt ggagacggat ttgttgagac tgaggcgttg gaaaccaatg
     9601 cgccccgttt gtccgcagcg cagcgatgcg gccataaatc cgcgtctgga atggctcgtc
     9661 gcgaaggtac aggctatcct gctcttcgag cggatatggc gcgcgatctt gccaccgctc
     9721 gtggtaacgg gtgccttcgt tagcatttct tggaccggag tctggctcga cgcgccccat
     9781 tgggcacgcg ggctcggcat tctcgttttg gcgcttggcc tgaccgccgc cttgctgcgc
     9841 ctgcgcggat ttcgctggcc atcccgcaag gaagctcttc agcggattga ccagacttcg
     9901 ggccttagcc tgcggccggc agccgttatt gccgaccggc tcggcaatgg cgtcaataac
     9961 ccggtgaccg tggcgctatg gaaccttcat cgccgccgcg cggaacaagc tatgctttgg
    10021 cttcgtaccg ggggaccctc accgcgcgcc gccgagctcg accggtatgc tttgcgggcg
    10081 atcgtcattg tcgcgcttat tgccaccggc tttgtcgcag gcccagaaag atatgcccgc
    10141 atcgccgcgg ctttcgattg gagttttacc ccgatcagca ggacggaaag ccgcgtcgat
    10201 gcctggatcg atcccccagc ctatactggc aagatgccaa tcgtgctgaa cctcgcaaga
    10261 agtcgaagct tcgttatgcc ggaaggcccc cagcgcattg aagcacccgc acgttcgatt
    10321 gtcgttgttc acgctccaga gggcaaactc gacatagagg taaaaggtgg gcttacgaga
    10381 tctgtaacgg acagtatcac agccgcgaaa gccaatgggg ccccccccgc caaaccagct
    10441 gacggcacgc gcgaaggcca aatagaaacc cgtctggttc tgcgtggtga tgcgacgtta
    10501 acgattcgca gttctggcac ccatctcggg a
//
LOCUS       DATMPU010000315         2921 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_3656923,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000315 DATMPU010000000
VERSION     DATMPU010000315.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 2921)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2921)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2921
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3656923"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            62..1060
                     /locus_tag="VNY06_07205"
     CDS             62..1060
                     /locus_tag="VNY06_07205"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024967.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HWX14622.1"
                     /translation="MARSIPIDSPRHGAGLPKGAETRQIGERPDSYLVMSVSTETRLG
                     SADSIRITEVFQLEEGNRIATRLLRAAKEGRLASGAFRHIRETVREYFYRLMLRQPFA
                     RSPMVPCVSLHGDFTVSPVCVLVKQSLDRALASIDGPPDFVREIEGMSGQKYRTFINC
                     LVGHHPDARYLEIGSWRGSTATAALCGNSVKALCIDNWSQFGGSRLSFLENIERVLSP
                     SIDFRFVEDDFRRVDYSALGRFNIFLFDGPHEEKDQYDGVTVARPALDKSFILIVDDW
                     NWRKVRLGTFRAIRDSHYLILASVEIRTTLDNSHARAAGRQSDWHNGYFIAVLVAN"
     gene            1262..2749
                     /locus_tag="VNY06_07210"
     CDS             1262..2749
                     /locus_tag="VNY06_07210"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HWX14623.1"
                     /translation="MSTELQPDLCVIGGGPGGLAVALGAAAAGKSVVVVEKSVLGGRR
                     LSESIPRHVLLAASRAADWVRRATDFGIKAQEPQFDFTRVQQQIATAAAAIAPNYSQA
                     RLEAMNIKLIRASGRFIGPDTCEAGGVNIKARSFIIATGAVARRLPIQGLDAVRPLDG
                     AALCTLGRPPGRLIVIGAEPDGLALAQAFRRLGSEVTILAGQALFTGEDEELAGPVRK
                     GFVRDGIMIHEGVRVTRIEPRDGSVRVLFAGAGHETAVGGSHILLAAGRAPAVEGLGL
                     AAAGVRYDENGIETNSRLLTSNWRIHALGAAVKGEQQEGAGEWHAAHVLRTILGLQGG
                     KLVREATARVVWTSPSIATAGVLEAQARATHRHISVLRWPLAETEWARIESQPGGHVK
                     LITTPAGTILGAGVVGFRAEELIPLFTLAISKRTTTQDIASIMLPYPALASGARSASM
                     TFQDRKSEVKFHQLLRAMSQVIERQIGEFREWAGGLGGKARGIFR"
BASE COUNT          599 a          790 c          897 g          635 t
ORIGIN      
        1 gccgcgggat cactcgttga agcgaggcgc ttagtcagcc atcgccggaa tcccggcacg
       61 ggtggctcgt tccatcccca tcgacagccc acgccacggc gcaggattgc ctaaaggcgc
      121 ggaaacgagg caaattggcg aacgacccga cagctatctg gtaatgtcgg taagtacgga
      181 gactagactc ggttcagcgg actcgatccg cattacggag gtctttcaat tggaagaagg
      241 caatcgtatt gcaacgaggc tgttacgcgc agccaaagag ggcagacttg ccagcggagc
      301 gttccggcat attcgcgaga ccgttcgcga gtacttttat agattgatgc tacgtcaacc
      361 tttcgctcga tcccctatgg ttccgtgtgt ttccttgcac ggggacttca cagtctcccc
      421 ggtgtgcgta cttgtgaagc aatcactcga tcgggcactt gctagtatcg acggtcctcc
      481 ggatttcgtc cgtgaaattg agggtatgtc gggacaaaaa taccggacat ttataaactg
      541 tcttgtaggt caccatcctg atgcgcggta tctagagatt ggttcgtggc gtggttcgac
      601 ggcaactgcc gcgttgtgtg gtaattcggt aaaggccctt tgcatcgata actggtcgca
      661 atttggaggc tccaggttaa gcttcttaga gaacatagag cgcgttctat ctcccagtat
      721 cgattttcga ttcgtcgagg acgatttccg acgtgttgac tattcggcgc ttggaaggtt
      781 caatattttc ctttttgacg ggcctcacga agagaaagat caatatgacg gcgtcacagt
      841 tgcgcggcca gccttggaca aatcgttcat cctgatcgtt gacgattgga actggcggaa
      901 agttcgactc gggactttcc gagccattag ggattcccat tatttgattt tggcttctgt
      961 tgagatccgc accacgctgg ataattccca tgcgcgcgcg gccggacggc agagtgattg
     1021 gcacaacgga tattttatcg cggttctcgt ggccaattga tacaatcgac gccaaatcag
     1081 tcgttctaag gtctcaaagc cgtgcctggc tatgaaattt ggaaactcga attcagctgc
     1141 ctttactaag ctcccctctc agcgcggcgc gatatatcag gcgaacaccg aacgggctat
     1201 cccgatttcg tccattcggg ggttgacgga cgatacatca gaggattcag aagagctctc
     1261 gatgagcaca gaactgcaac ctgatttatg tgttatcggc ggcggcccgg gtggcctggc
     1321 agttgcgctt ggggcagcgg cggcggggaa gtccgttgtg gtcgtcgaaa agagtgtgct
     1381 tggcggccgc agactatccg aatctattcc acgccatgtc ttgctcgcgg cgagcagggc
     1441 cgcagattgg gtccggcgcg ccacagattt tgggatcaaa gcccaagaac cgcagttcga
     1501 tttcacgcgc gtgcaacaac aaatcgccac cgcggctgcg gcaatcgcac caaattattc
     1561 gcaagcgcgc ctcgaggcta tgaacataaa gctcattcga gcgtccgggc gcttcattgg
     1621 gcccgatact tgcgaggccg gcggcgtaaa catcaaggcg cggtccttca ttatcgcgac
     1681 cggagctgtc gcgagacgat tgcccataca agggctagac gccgtccggc cgctcgatgg
     1741 tgccgcgttg tgtacgctgg gccgtccgcc agggcgcctc atcgtcattg gggcagagcc
     1801 cgacggactt gctctcgcgc aggcttttcg ccggcttggc agcgaggtga ccattctcgc
     1861 gggccaggcg ctctttaccg gggaagatga agaattggcc gggccggtgc gcaaaggctt
     1921 tgttcgggac ggcattatga tccatgaagg cgtgcgggta acccggatcg agccgcgtga
     1981 cggcagcgtt cgcgttcttt tcgcaggtgc aggccatgag acagccgtcg ggggctcgca
     2041 tattcttctc gccgcaggca gggcgcctgc cgtcgagggc ttgggccttg ccgccgcggg
     2101 agtacgttat gatgaaaatg gcatcgagac caattcccgg cttttgacaa gcaactggcg
     2161 catccatgcg ctcggcgcgg ctgtaaaggg cgagcagcag gaaggggccg gcgaatggca
     2221 tgccgcccac gttttgcgca ccattctcgg cttgcagggc gggaaactgg tcagggaagc
     2281 gacggcgcgg gtcgtttgga cctctccgtc catcgccacc gcgggagttt tggaagcgca
     2341 agcacgggcg acgcatcgcc acatttctgt gctccgctgg ccgctggccg agactgagtg
     2401 ggcgcgcatc gagagccagc ccggcgggca cgtcaagctg atcaccaccc cggcgggcac
     2461 aatactcggc gccggagtcg tgggctttcg agccgaggag ttgattcctc tgttcacgct
     2521 tgccatatcg aaacgcacga ccactcagga catcgcgtct ataatgcttc cctatccggc
     2581 tttggcaagc ggggcgcgca gtgccagcat gacgtttcag gatcgcaagt ccgaggtaaa
     2641 attccatcag ctcctgcgcg caatgagtca ggtgatcgag cggcaaatag gggaattcag
     2701 ggaatgggca ggggggcttg gcggaaaagc ccggggtata ttccgctagc cgatgaaata
     2761 tatgaacacg cgcaagcgac atatctgtgg caagcgatcg tgtaaggaaa gcatatttga
     2821 aagcggcgca agtactttgc tctgaggccg ccgccgaggg tggccacgcc cttcgtctgg
     2881 cgtgggttcg gatagggttg ccatgagcga tcttgtgata c
//
LOCUS       DATMPU010000316         7357 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_1787266,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000316 DATMPU010000000
VERSION     DATMPU010000316.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 7357)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7357)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7357
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1787266"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            355..2745
                     /locus_tag="VNY06_07215"
     CDS             355..2745
                     /locus_tag="VNY06_07215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020174653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HWX14624.1"
                     /translation="MREFRLRTGNPGQARTASPPGRQRRLRPLTLGIERIGLISLRYP
                     VTVALIAILLVIAAGLGIFRLKVDDSLSQLFRSENSEFHQYEEVTRQFPSSEFDVLVV
                     IEGKKLLERASVEKLRDLATDLQLVDGTRGIISIFSARQPPRGQEIPAPLFPDPLPQG
                     SAYEATISQVMNSEIIRGKLLSEDGKLTLMVVAVDPSAVENKRLSDVVGEIRKTLAED
                     LAGLHLTAELSGVPVMQLEIRNAVEHDRLVYNVMGFAAGCLIAIAFFRRISFMIIAAG
                     PPLIAILLALGTLGWLDFRLNMFLNVMTPLIMVISFSDSMQLTFAMRDRLHAGQSKSE
                     ALQNAILIVGPACVLTHATAAVSFLALQISDSDLIRSFGEAGFISTVIALAVVLILMP
                     LLAVILLRGEGAFAARGANSDFGVEQLRRFCGYIAACMVRRPGLYSLAGILVVAVLGV
                     VYAGLEPRYRLADQVPNRQQAIAASNRLDAKLTGANPIDVLIEFPPHTSLYAPETLRV
                     ISQVHRILEGQAGIGNVWSLETLRRWLVKANQPDVATLKEYVGILPKHLTRRFISAGE
                     DAVVVSGRIPDADASQLLPVIRALDQSLAIVRAQHSGYRISVTGLSAIAAWNSANMIH
                     KLNRGLTIEFIFIAAFIGAAFRSFAVTLMSFLPGIFPVVACGAILRLTGQGLQFASVV
                     ALIVSFGLGLSATIHFLNRMRLEEEQDSDPAIAIERATVLVGPALILTSIVLACGLAV
                     TVLSSLPSLCLFGWLSALAMIAALTADLFILRPVAMFLHRFSFRAESWRRQRTADS"
     gene            complement(2765..3262)
                     /locus_tag="VNY06_07220"
     CDS             complement(2765..3262)
                     /locus_tag="VNY06_07220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012385039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX14625.1"
                     /translation="MKPIFSTEAAAIVISGLLLTSIPGMADPGPGGPFLGMSGRWSGA
                     GTITMTNGSRERIHCKAAYTVTATGRAIQQTLNCASDSYRLDINSNVVSEAGALSGSW
                     SEGSRGVSGNVTGRANGAEIVASVVGGGFTASLNVRTQGDRQSVTIRPQGATDVAAVS
                     IALRK"
     gene            complement(3470..4747)
                     /locus_tag="VNY06_07225"
     CDS             complement(3470..4747)
                     /locus_tag="VNY06_07225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX14626.1"
                     /translation="MSVALLILLGLISLCPAVITLDGAVLSSFIPLLVSAGLIVVSVK
                     LPEGEAQHLLTVVSRPFVVIAAFPAVLMIVQILPLQFLANPIWTSVNAGFPRGITGSV
                     SVDTGATAIALARYLSVVGAVVFAAAVASDRFRAEVVLTGVLAAIVLISLAFLNHDLV
                     SPRFFVRREEALDCACLGITLSAACGNLIFERYETRRYKSNQNQKKYLFGALACLVAF
                     SICAGAVTVTRSGSLIFAASSGFLTFCATVVIRRFGLGRFGAVAIGSTAVVIATALVT
                     VAATDPDPRFAFVKKDAAAIEITQRMLGDAPVLGDGAGTFAALVPIYRSSGTGSKEVE
                     AVTAAAQLSIEMGRGALWIAIMTASFVVYVLLRGAASRGRDSFYAAGAGACIVTLMNL
                     AFVNVGLTGHALTLLSGIILGLGLPQSKSRIAS"
     gene            complement(4820..5827)
                     /locus_tag="VNY06_07230"
     CDS             complement(4820..5827)
                     /locus_tag="VNY06_07230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003611929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HWX14627.1"
                     /translation="MGSLSPGTGPKRRIWVAGHRGMVGSAILRRLARENAEVLTVGRQ
                     DVDLREQLDVRRWVAQAKPDVIILAAAKVGGILANDSYPADFLFDNLAIETNVIHAAH
                     LANVERLVFLGSSCIYPKLAPQPIAEEALLTGPLEPTNEWYAVAKIAGIKLCQAYRRQ
                     FGRRYISVMPSNLYGRNDNFDLSTSHVLPALIRKFHAAKMAGKAEVVIWGTGTPLREF
                     LYVDDLADAIVFLMDRYDSGEPINCGAGFDVTIRELAELIGRVTGFEGALVFDKTKPD
                     GTPRKLMDSSRLSALGWRPKTSLEDGVRGVYMWYVQTMADEPADAVRDDFLQRWPQSA
                     P"
     gene            complement(5838..6896)
                     /gene="gmd"
                     /locus_tag="VNY06_07235"
     CDS             complement(5838..6896)
                     /gene="gmd"
                     /locus_tag="VNY06_07235"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012589135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HWX14628.1"
                     /translation="MTAKKVALITGVTGQDGALLAELLLSKDYIVHGVKRRSSLINTD
                     RVDHLYHDPHESGVNFLLHYGDLTDATNLIRIVQEVQPDEIYNLAAQSHVQVSFETPE
                     YTANADALGTLRLLEAIRILGLCQKTCFYQASTSELYGKVQAIPQNEATPFYPRSPYA
                     AAKLYAYWITINYREAYAIHASNGILFNHEGPTRAETFVTRKITRAVAAIHLGFQDKL
                     FLGNLDAKRDWGHARDYVEGMWRILQQPEPDDYVLATGETHTVREFVERAFAEVGTEI
                     GWKGKGIAEQGFDKRDSRILVEIDPRYFRPTEVDLLLGDPSKAWKKLGWRHTVTFPQL
                     VSDMIASDLVEMKKRGRI"
     gene            complement(7050..>7357)
                     /locus_tag="VNY06_07240"
     CDS             complement(7050..>7357)
                     /locus_tag="VNY06_07240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015928842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphate acyltransferase"
                     /protein_id="HWX14629.1"
                     /translation="AHATDREADGEMQANTALSQALRNLVLPSSRLKGEANILIMPNL
                     DAANIAYTMTMIMADTSPVGPILVGAAKPAHILTPSVTVRGLINMTAVSVAEAQDGT"
BASE COUNT         1614 a         2123 c         2012 g         1608 t
ORIGIN      
        1 catgagcgcc gcctgccaag cgtggtcccc gacggcaggc gggaatctgc cagcacccaa
       61 catattgttg tgatcacggg ataaaatccg gccctgcccg gggttgttga tgatacgcag
      121 gcacagcgcg gaaggttggc acaagcatta tttggcagcc tgtagccagg ctcccttgct
      181 gttctaatgg tcaagcgcat ggcgcgcgtt cgctatcgat actcattctt cccgaatcct
      241 gttgcccagc ctggaacgga ttccggtcca gggatggcgc cgggcgcgag gattcgcggc
      301 ttggatggtc ggatagcaaa atcactctct gggccggact aagggacggc caagatgagg
      361 gaatttcgtt tgcgaaccgg gaatccaggt caggccagga cagcgtctcc acccggaaga
      421 caacgaaggc tgcgtccgct tacccttgga atagagcgga taggactgat ttctctgcgc
      481 tatcctgtca ctgtggcgct tatcgcaatt ctgctcgtta tagccgcggg actgggcatt
      541 ttcaggctca aggtcgacga ttcactgagc cagcttttcc gctcggaaaa ttctgaattc
      601 catcaatatg aggaagtaac gcgacaattc ccgtcgagcg aatttgatgt tctcgtcgtt
      661 atcgaaggaa aaaaattgct cgagcgcgca tccgtcgaga agttgcgcga tctcgccacc
      721 gacctgcagc tggtcgacgg cacacgcgga atcatttcga ttttctcggc tcgccagcca
      781 cctcgcggcc aggaaattcc ggcgcctctg tttcctgatc cacttcccca gggatccgcc
      841 tatgaagcga caatcagcca ggtgatgaat agtgagatca ttcgaggcaa gctcttatcg
      901 gaagacggaa agctgacttt gatggttgtc gcagtcgacc cgtcggcggt tgaaaacaag
      961 agacttagtg acgttgtcgg cgagatccgc aagacgctcg ccgaggattt ggccggactg
     1021 catttgacgg ctgagctttc cggcgtaccg gtcatgcagc tcgaaattcg caatgcggtc
     1081 gaacacgaca ggctggttta caatgtcatg ggatttgctg cgggttgttt gatcgcgatc
     1141 gccttcttcc gccgtatctc gttcatgatc atcgcggctg ggccgccgct gattgcaata
     1201 ttgcttgcac tcggcacgtt aggctggctc gattttcgcc taaatatgtt cctcaatgta
     1261 atgacaccgc tcataatggt gatcagcttc tccgacagca tgcagctcac cttcgccatg
     1321 cgcgaccgct tgcacgcggg acaaagcaag agcgaggctt tgcagaacgc gattctgatt
     1381 gtcggaccgg cttgcgttct tacgcacgcg accgcggctg tttcgtttct cgccttgcaa
     1441 atttccgatt cggacctgat acgaagcttt ggtgaggcgg gctttatttc gacggtcatt
     1501 gccctagccg ttgtcctcat tctcatgccg ctcctcgctg tcattttgct acgtggcgag
     1561 ggcgcttttg cggcgcgagg cgccaactcg gatttcggcg tggagcagtt gcgccgattc
     1621 tgcggctaca tcgccgcatg catggtccgt cgtccggggc tttacagcct ggccgggatc
     1681 ttggtcgtcg cggtgctcgg cgtcgtctat gcaggtctcg aaccgaggta ccggctcgcc
     1741 gatcaagtcc ccaacagaca gcaggcgatt gctgcaagca atcgtctcga cgccaaactt
     1801 accggcgcca atccgatcga tgtgctgatc gaatttccgc cccacacttc cctctatgcg
     1861 ccagaaactc tcagggtgat ctcgcaggtg catagaattc tcgagggaca ggctggcatc
     1921 ggcaatgttt ggtcgctcga aactttgcgc cgctggctcg tgaaggcgaa ccagccagac
     1981 gtcgccactt tgaaggagta tgtcgggata ttgccgaagc atttgacacg ccggttcatt
     2041 tcggctgggg aggacgccgt cgttgtctcg ggacggatac cagacgccga cgcaagccag
     2101 cttcttcccg tcatccgcgc tctcgatcaa tcgcttgcaa tcgtgcgggc acagcacagc
     2161 ggctatagaa tttcggtaac aggactttca gcgattgctg cctggaacag cgccaatatg
     2221 attcataagc ttaatcgtgg cctgacgatt gaattcatct tcattgccgc gttcatcggc
     2281 gcagccttcc gttcattcgc tgtcacgctg atgagctttc taccagggat cttcccggtt
     2341 gttgcctgtg gggcaatcct gcgcctgacg ggacaaggct tgcagttcgc cagcgtcgtc
     2401 gcgctcatcg tttctttcgg actggggttg agcgctacga ttcacttcct aaaccgtatg
     2461 cgcctcgagg aagagcagga cagtgaccca gctatcgcga ttgaacgcgc gaccgtcctc
     2521 gttggccctg ctctcatcct aacctcaatc gttctggctt gcgggttagc ggtgacagtc
     2581 ctctccagcc tcccgtctct gtgcctcttt ggctggctta gcgcgcttgc catgatcgcg
     2641 gcgttaaccg ccgatctctt tatattgcgg ccggtggcca tgttcctgca ccggttttcc
     2701 ttccgagcag aatcatggcg ccgacagcga acggcagaca gctaatttta gcaaagacca
     2761 tcggtcactt gcgcagtgct attgacaccg cagccacgtc agtggctcct tggggcctta
     2821 tcgtgaccga ctgccggtcc ccttgcgtgc gtacattgag actggcggta aagccgccac
     2881 cgacaacgct tgcgacgatt tccgcgccgt ttgcgcggcc agtgacgtta ccagacacgc
     2941 cccgcgagcc ctcggaccac gaaccggaca gcgcaccggc ttcggataca acgttgctgt
     3001 tgatatccag cctgtagctg tcgctcgcgc agttcagagt ttgctgaatt gctctgccag
     3061 ttgcggtgac cgtataggct gccttgcaat ggatccgttc cctggaccca ttggtcatgg
     3121 tgatggtgcc agcgccggac cagcgccccg acattccgag gaacggaccg ccgggacccg
     3181 ggtcggccat gccaggaatc gatgtgagca gcagcccgga tataacaatg gccgcagctt
     3241 cagtcgaaaa tatgggtttc atgactttcg ctttcctaaa cgtaaaaggt taagagccaa
     3301 gcttcctctc gcgtgccaag ccaagcattg cggacccccc aacgcatctg cttaacctcc
     3361 acaagaatca tgcctcgcgc tgtatttaca tggttgttat ttcctcggag ttaagtacga
     3421 gacttataaa cttgctgcga tcccgcgccc gttcgagacc atcgccggct caggatgcga
     3481 tgcggctttt tgattggggg agcccaagcc ccaaaataat gccggacagc aaagtaagcg
     3541 cgtggcctgt gagaccaaca ttaacaaatg cgaggttcat aagcgtgacg atgcaggcgc
     3601 cagccccagc cgcgtaaaat gaatcccggc ctcgactcgc agcgccgcgt aaaagcacat
     3661 acacaacaaa ggacgccgtc atgattgcaa tccagagcgc cccccgtccc atctcaattg
     3721 aaagctgagc ggctgcggta acggcttcaa cctctttgga cccggtgccg cttgacctat
     3781 agatcgggac caacgcagca aacgtcccgg ccccatcgcc gagtactgga gcatcaccaa
     3841 gcatgcgctg ggtgatttca atggctgctg cgtccttctt gacaaaggca aatcttgggt
     3901 cgggatcagt cgccgcgaca gtcaccaagg ctgtcgcgat cacgactgcc gtgcttccaa
     3961 tcgcgaccgc gccaaatcgt ccaaggccaa accggcgaat cacgacagtc gcgcagaatg
     4021 ttagaaaacc cgatgatgcc gcgaagatca aggaccccga tcgggttacg gtaactgcgc
     4081 ccgcgcatat tgaaaaggcc actaagcaag cgagtgcccc gaacagatat ttcttttggt
     4141 tttggttcga tttgtaccgg cgggtctcgt aacgctcgaa gatgaggttc ccgcaggccg
     4201 ctgacagcgt aatcccgaga caggcacaat cgagcgcctc ttcacgcctc acgaagaaac
     4261 gagggctgac aagatcatgg ttcaggaaag ccagggaaat gagtacaatg gccgctagca
     4321 caccggtcaa taccacttcg gcccggaacc ggtcgctcgc aaccgcagcg gcgaacacga
     4381 ccgcgccaac caccgagaga tagcgcgcga gagcaattgc ggtggcacct gtgtccacgc
     4441 tgacgcttcc agtgatccct cgaggaaagc cggcatttac gctcgtccaa atcgggtttg
     4501 ctagaaattg gaggggcaat atttgaacga tcatcaaaac cgccgggaaa gcggcgataa
     4561 cgacaaaggg gcgcgagacc acggtcagca aatgttgcgc ctcgccctca ggtagcttga
     4621 ccgaaaccac gataagcccc gcggagacga gtaacggaat gaagctcgaa agcaccgccc
     4681 catcgagagt gatcaccgcg ggacaaaggc taatcagccc taacagaatg agaagcgcta
     4741 cggacatggg atgaagttgg gcctgaaagg aattggcact gattctggcc cgccttgata
     4801 gcgtaccgcc attggcaagt cacggtgcgg actgcggcca gcgttgaaga aagtcatccc
     4861 tcacggcgtc tgctggctcg tccgccattg tttgaacgta ccacatatag acgccgcgaa
     4921 caccgtcttc gaggctcgtt ttcggccgcc agccgagagc cgacaaccgg ctcgagtcca
     4981 taagttttcg tggcgtgccg tcgggcttcg tcttatcgaa aacaagtgcc ccttcgaatc
     5041 cagtgactct gccgatcagc tcagcaagct cgcggatggt tacgtcaaat cccgcgccgc
     5101 agttgatcgg ctcgccgctg tcgtaccggt ccataagaaa cacgatggcg tcagccaagt
     5161 cgtcaacgta gagaaactcc cgcaaggggg tcccggtgcc ccaaataacg acttctgcct
     5221 taccggccat cttggcagcg tggaactttc gaattaacgc aggcaggaca tggctcgtcg
     5281 aaagatcaaa gttatcgttg cggccataga gattgcttgg catgaccgag atatagcgcc
     5341 tgccaaattg ccgccggtac gcctgacaaa gtttgatgcc ggcgattttc gcgactgcat
     5401 accattcatt ggttggctca agcggaccgg ttaacagcgc ttcctcggcg atcggctgcg
     5461 gcgccaattt cgggtaaatg cacgacgatc cgagaaagac cagccgctcg acattggcaa
     5521 gatgcgcggc gtggatgaca tttgtctcta tcgccagatt atcaaagagg aaatcggctg
     5581 gataagaatc attggcgagg attccgccaa ctttggcggc agcaagaatg ataacatctg
     5641 gcttggcctg cgccacccat cgccgtacat caagctgttc gcgcagatcg acatcctggc
     5701 gccccactgt caggacttcc gcattttccc tggcgagccg ccgcaggata gccgagccca
     5761 ccatgccgcg atgcccggcg acccaaattc gccgcttggg cccagttccg ggtgacaaac
     5821 tgcccatgaa cgacccctca aatccggcca cgctttttca tctcaaccaa gtcgcttgca
     5881 atcatgtctg aaacgagttg cggaaaggtg acggtgtgcc gccagccaag ttttttccat
     5941 gctttcgacg gatcaccaag aagaagatca acctcggttg gccgaaagta acgcggatca
     6001 atctcgacaa ggatccggct gtctcttttg tcgaagcctt gctcggcaat tcccttcccc
     6061 ttccacccga tctcagttcc gacttcggcg aaggcccgct cgacgaactc tcttacggta
     6121 tgggtctctc ctgtagccaa gacataatcg tccggctcgg gctgttgcag gatgcgccac
     6181 atcccttcga cataatcgcg cgcatgtccc caatcgcgct ttgcatccaa attccccaaa
     6241 aacagtttgt cctggaatcc taaatggatc gcagccactg cacgggtaat ctttcgcgtg
     6301 acaaaggtct cggctctggt tggcccttcg tgattgaaca ggattccgtt cgaagcgtga
     6361 atcgcataag cctcacggta attgatcgtg atccaatatg catagagctt tgcggctgca
     6421 taaggcgaac gcggataaaa aggcgttgcc tcattttgag gaattgcctg aaccttaccg
     6481 tagagttccg atgtcgaggc ttgataaaaa catgtcttct gacagaggcc caggatacga
     6541 atggcctcga gaagtcgaag cgtgccaagc gcgtcggcgt tagcggtata ttccggcgtt
     6601 tcaaagctca cctggacatg gctttgcgcg gcgagattgt agatttcgtc gggctggact
     6661 tcctgcacaa tgcgaatgag gtttgtcgca tcggtaagat caccatagtg cagcagaaag
     6721 ttgacgccgg attcatgcgg atcgtgatac aaatgatcaa cacgatcggt attgatcaat
     6781 gacgaccggc gcttcacacc atgcacaata tagtctttgg aaaggaggag ttccgccagc
     6841 agagcgccat cctgtccggt aacgcccgtg atcaacgcaa ctttttttgc tgtcatgtga
     6901 tgtaaatatc cttcaatggg cggggcgcga aacggttgtt cgattttcaa cgccaagata
     6961 tcacaaaatc aatgctccga acccgagaaa atgcgttggg gaactcgttc gatcgcttga
     7021 tccagctcag aacctcctct caggctgacc tacgtcccgt cctgcgcctc cgcgaccgaa
     7081 accgcagtca tattgatgag gccacgtacg gtcacggaag gcgtcaaaat atgggcgggc
     7141 ttcgctgcac caacaaggat cgggccgacc ggcgaagtat cagccatgat catggtcatt
     7201 gtataagcga tattggcggc atcgaggttg ggcatgataa gaatatttgc ctcgcctttc
     7261 agccgcgaag aaggcaaaac gaggttacgc agcgcctgcg acaaagcggt attggcctgc
     7321 atttccccat cggcttcacg atctgtggcg tgcgcac
//
LOCUS       DATMPU010000317         3335 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_1788666,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000317 DATMPU010000000
VERSION     DATMPU010000317.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 3335)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3335)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3335
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1788666"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            96..731
                     /locus_tag="VNY06_07245"
     CDS             96..731
                     /locus_tag="VNY06_07245"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012592759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HWX14630.1"
                     /translation="MNRILVLLRHGQSEWNLKNLFTGWKDPNLTDRGVEEARVAGRCL
                     KAQGLSFDRAFSSNLIRARRTLQLVLAELGTPELEIVSDRALNERDYGVLCGLNKDDA
                     IRRWGDEQVDLWRRSYNVRPPGGESLKDTVARVLPYYCQKILPAVLSSHRVIVAGHGN
                     SLRALVMVLDGLTPETIPELELATGIPLIYRLRPDSLVATRELLDPKRFVA"
     gene            765..1409
                     /locus_tag="VNY06_07250"
     CDS             765..1409
                     /locus_tag="VNY06_07250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009763621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate-dependent dioxygenase AlkB"
                     /protein_id="HWX14631.1"
                     /translation="MQDLLEPAEAAAIALAPGLTLFRRYLDDTAWALASEIEKLVSLA
                     PWFLPRMPRSGRPFSVKMTNCGRLGWVSDRDGGYRYQATHPVTGEAWPPIPVSVLDIW
                     RAVASHPHLPEACLVNFYDSSAKMGLHQDRDEAELAAPVVSISLGDSCSFRCGGLRRT
                     DPAKKLELRSGDVVVMGGPARLIFHGVDRIYPGTSDLLPGGGRINLTLRRVRRP"
     gene            1927..2748
                     /locus_tag="VNY06_07255"
     CDS             1927..2748
                     /locus_tag="VNY06_07255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012592761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HWX14632.1"
                     /translation="MKLSLIQMNSINDKAANIVSATGLVERAVAEDRPDWICLPECFD
                     FLGGDRRAKWAAAESFPGGPAYSAMQRLASNHHIYIHAGSILEKPESGERIHNTTVVF
                     DRNGAEVARYRKIHMFDITAPDGTRYHESAAFAPGQAVVTYGCEDMTIGCSICYDLRF
                     PALFQALSAKGAQMIALPAAFTLQTGKDHWEVLCRARAIETETYFCAAAQTGTHAEGK
                     EMRHTYGHSLVIDPWGHVIAKASDGVGIVSARIELDRVKKVRTQIPVAQHKVRFD"
     gene            2766..>3335
                     /locus_tag="VNY06_07260"
     CDS             2766..>3335
                     /locus_tag="VNY06_07260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012592762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWX14633.1"
                     /translation="MKGLSHSLPRTAGLLLAVLAIELTANASPADKAQPKMVWHRGEL
                     GDPGSLDPHKATTLIESNVLDELFEGLVALDARGEIIPGVAESWTVDQSGTVYEFRLR
                     RDAKWSNGDRLVAEDFVYAFQRLMAPSTGAPYANILYTLKNAERINKGELPVESLGAR
                     ALDDRTLEITLEQPAPYFIAQLPHMTAKPL"
BASE COUNT          699 a          969 c          949 g          718 t
ORIGIN      
        1 tatagtcgca atggcaatgt tttgttgatt gaaagatttc atcccgttcc caaacgcgct
       61 agctatgcag tgcaaaaacc cagcaaggtt aggatatgaa tcgcattctc gtgctcctga
      121 gacacggtca aagcgaatgg aatttgaaaa atctctttac cggctggaaa gaccccaatc
      181 tgaccgaccg aggggtggag gaagcgcgtg tcgccggacg ctgcctcaag gcgcaaggcc
      241 tatccttcga ccgggccttt agttccaacc ttattcgcgc gcgccgtacc ttgcaattgg
      301 ttctcgccga gctcggcacg ccagagttgg agatcgtgag cgaccgcgcg ctcaatgagc
      361 gcgactatgg cgtgctgtgc ggtctcaaca aggacgatgc catcaggaga tggggcgacg
      421 aacaggtgga cctttggcga cgatcttata atgtgcggcc tccgggcggc gaaagcttga
      481 aggatacggt ggcccgggtc ctcccttatt actgccagaa aattctacct gccgtgctga
      541 gtagtcaccg cgtgattgtc gccgggcatg gcaattcgct gcgcgcgctt gtcatggtcc
      601 tcgacgggct aacgccggag acaatacctg aattggaact cgcgaccgga atccctctta
      661 tctaccggct gaggcccgat tccttggttg caacgagaga gttgctcgac ccgaaacgtt
      721 ttgtagcgta aactcgccaa attcgcgcca ccaaaaacct cgttatgcag gatctgctcg
      781 aaccagctga ggcggcggcc attgcgttgg cccctgggct tacccttttt cggcgctatc
      841 tcgatgacac tgcatgggcg cttgcctcag agattgagaa acttgtctca ttggcgccct
      901 ggttcttgcc ccgcatgccg cgctctggcc ggcctttctc ggttaaaatg accaattgcg
      961 gcaggcttgg ctgggtttcc gaccgggatg gaggctaccg ctatcaggca acgcatcccg
     1021 ttactggcga ggcttggcct ccgatccccg tctccgtgct cgatatctgg cgcgccgtgg
     1081 cttcacaccc gcatttgcca gaagcctgcc tcgtcaattt ctacgattcg tcggcgaaga
     1141 tgggcttgca ccaggatcgc gacgaagcgg agctcgccgc gccggtggtg tcgatttcgc
     1201 tcggcgatag ctgctctttt cgttgtggcg gcttacgccg gaccgatccg gcaaaaaagc
     1261 ttgagttgcg ctccggagac gtcgttgtga tgggcggccc ggcccggctc atctttcatg
     1321 gcgtagacag gatctatccc ggcacgtcgg acctgctgcc tggcggcggg cggatcaatc
     1381 tcaccttgcg gcgtgtgaga cgcccctgac ttatccgtgt cggattggcc gttccgtcag
     1441 cctgtcacaa ttgcgttgcg atggcggcga ggcatgccgg catcaacgcc tcgggaaggg
     1501 tcaaactcct agtcccgtgc tcgtctcgaa aacttggcgc cgcaggcctt tgctcgctga
     1561 ggctttcagt cacccggagg gtgcggccgg ccattcggca agctccggat taccgctcgt
     1621 cgccaattgc cgcagcatgt caggggggat tataaaacag ccgggacagc ttcccacatt
     1681 taaagcggaa cgtgttttac gcatggccac cgccaaatag ctcaccgggc gaggtcaccg
     1741 ctctgcaatt caaacgttcg cgccagcatt tcgcgccgct ctcttaagct atgataaaaa
     1801 attctcctgt catcagagat tgctgaataa gaatacgatt gttgattaaa cagcggccac
     1861 aagaatcgct gggaaagagc gccgcgagtg tgatagacgc aagcgcaatc attgctggag
     1921 catttcatga agctttctct gatccagatg aactcgatca acgacaaagc cgccaatatc
     1981 gtgtctgcca caggtctcgt tgagcgtgcc gtcgctgagg accggccgga ttggatttgc
     2041 ttgcccgaat gttttgattt tcttggaggc gatcgtaggg ccaagtgggc ggcagccgaa
     2101 tcctttccgg gtggtccggc ctattccgcg atgcagcggc tcgccagcaa ccatcacatc
     2161 tatattcatg ctggctcgat cctcgaaaag ccagagagcg gagagcgcat tcataataca
     2221 acggtcgtct tcgaccgcaa cggcgcagaa gtcgcgcgtt accgtaagat ccatatgttt
     2281 gatatcactg cgcctgacgg aacaagatat catgagagcg ccgctttcgc gcctggccag
     2341 gccgttgtga cctatggctg cgaggacatg acgattggct gctcgatctg ttacgatctg
     2401 agattcccgg ctctcttcca agcgcttagc gccaaaggag cgcagatgat cgccctgccc
     2461 gctgccttta ccttgcaaac cggcaaggac cattgggaag ttctctgccg cgcgcgcgcg
     2521 attgaaaccg agacctattt ctgcgccgcg gcacaaacgg gcacccatgc cgaaggaaag
     2581 gaaatgcgcc acacatacgg ccattccctc gtcatcgatc cctggggcca tgtcattgcc
     2641 aaggcatcgg acggtgtggg catcgtgtcg gcaagaatag agcttgaccg ggtcaaaaaa
     2701 gtccgcacgc aaatcccagt ggcgcagcac aaagtgcgct tcgattgagc gcgagacaca
     2761 gcaaattgaa aggcttgtcc cactccctgc cgcgcaccgc cgggctcctc ctcgcagtcc
     2821 tggcgataga gctgacggca aacgccagcc ccgctgataa ggcccagcca aaaatggtgt
     2881 ggcatcgcgg cgaacttggc gatccgggat cgctcgatcc ccacaaagcc acgaccttga
     2941 ttgaaagcaa tgtactcgat gagctttttg agggccttgt cgccctcgac gcacggggcg
     3001 aaatcattcc gggcgtcgca gagagctgga ccgtcgatca gagcggcacg gtttatgaat
     3061 ttaggcttcg ccgcgacgcg aaatggtcga acggcgatag gttggtggct gaagattttg
     3121 tctatgcatt ccagagattg atggcgccca gcactggcgc tccttacgcc aacatccttt
     3181 atacgttgaa aaatgcggaa aggattaaca agggcgagct cccggtcgaa tctttgggcg
     3241 cgcgcgcctt agacgaccgg acgctcgaga tcacccttga gcagccagca ccctatttta
     3301 tcgcacagct tccccatatg acggccaagc cgctg
//
LOCUS       DATMPU010000318         3865 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_2719289,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000318 DATMPU010000000
VERSION     DATMPU010000318.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 3865)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3865)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3865
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2719289"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            115..570
                     /locus_tag="VNY06_07265"
     CDS             115..570
                     /locus_tag="VNY06_07265"
                     /inference="COORDINATES: protein motif:HMM:NF019118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HWX14634.1"
                     /translation="MPYLHASAVVIGEAGVLIRGPSGSGKSTLAYSLIAAAEESGYFA
                     RLVGDDRIRISSHGERLIAHGHASILGKIERCSQGVIEVPFLAAAVLRLAVQLTGTDE
                     TALRYPEPDHDHILLAGSKLPCLSVRQDAAPAALSLAILADLRLRRIIF"
     gene            1009..1410
                     /locus_tag="VNY06_07270"
     CDS             1009..1410
                     /locus_tag="VNY06_07270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003516318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HWX14635.1"
                     /translation="MIGIILVTHGCLAAEFRAALEHVVGPQNQIESIAIGPEDDIEQR
                     RQDIVAAVAKVDSGAGVIVLTDMFGGTPSNLAISIMNGANIEVIAGINLPMLIKLASV
                     RDTASLEQAVIQAQDAGRKYIYVASRVLSGK"
     gene            1407..1742
                     /locus_tag="VNY06_07275"
     CDS             1407..1742
                     /locus_tag="VNY06_07275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020173392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HWX14636.1"
                     /translation="MIKDDAVPDEPSDCPDLEPPGDVLVRTLEIVNRKGLHARATAKL
                     VQCIDGFDAVVTISRCGETVGGSSIMGILTLGAGQGMTITVTAAGPGAAQAMKAIAAL
                     IANRFGEDE"
     gene            complement(1774..2781)
                     /gene="pgi"
                     /locus_tag="VNY06_07280"
     CDS             complement(1774..2781)
                     /gene="pgi"
                     /locus_tag="VNY06_07280"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF001211.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HWX14637.1"
                     /translation="MTNARSARQAVAARLGEMAVGDHFCAVSTQLDKIAAFGIRSDRV
                     FGFWDWVGGRYSIWSSIGLLLAIGIGKEKFEEFLAGGQDIDIHFTKAPLERNIPVLMA
                     LLGIWYRNIWGYSTHAVIPYDERLARFPAYLQQLEMESNGKSVDLSGSRVDFETAPII
                     FGEPGTNGQHAFFQLLHQGTSVVPIDFFVAANPLDADVEHHQLLLANCLAQSQALMKG
                     RSIAEAREGLAAQNVSPRDIEALAPHKVFEGNRPSSTFLYRRMSPRVLGQLIALYEHK
                     VFVQGVVWNINSFDQWGVELGKELANNLTPIVDGRSPAGPGIDASTLGLIAAARAFRE
                     G"
     gene            complement(2778..3419)
                     /locus_tag="VNY06_07285"
     CDS             complement(2778..3419)
                     /locus_tag="VNY06_07285"
                     /inference="COORDINATES: protein motif:HMM:NF012562.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX14638.1"
                     /translation="MAREQGANSLAALEAHKLAVGRQTIADFFRDDPGRFSQFHVEAD
                     GLLFDFSKHKINQTTLGLLIDLAREADVGARRAALFSGEPVNATEHRPALHMALRNLS
                     GNPMFAAGRDVMPEVLAERARMEAFAEAVRNGNVKSTSGAPFTDIVNIGIGGSDLGPA
                     MAVRALAPFVPRNLRLHTVANADGADLGDTLKLVPLETTLFIVCSKTLQRSRR"
     gene            complement(3585..>3865)
                     /locus_tag="VNY06_07290"
     CDS             complement(3585..>3865)
                     /locus_tag="VNY06_07290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012453958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ammonia channel protein"
                     /protein_id="HWX14639.1"
                     /translation="AIGTGILVAPYLGGTGLTDYTIKPGFASAGDYSMLPQVIIQTKN
                     VLFTLFWSGFGSAILYKFVDLIIGLRATKQAELEGLDITDHGERAYNQ"
BASE COUNT          828 a         1103 c         1112 g          822 t
ORIGIN      
        1 ccacccggtg atgacggcat gcctcccgtg cttggagcaa gattcctggt cttcctgccg
       61 aaagcccgaa agaccatccc ttagagagaa agccaatggg ccgggattgc gaagttgcct
      121 tatctccacg caagtgctgt cgtgatcggg gaggccggcg tgctcattcg cgggccatcc
      181 ggctccggaa aaagcactct cgcgtatagc ctaatcgcgg cagcggaaga atcaggctat
      241 ttcgcaagac tcgtgggcga cgaccgcatc agaatttctt cgcatggtga gcgcctgatc
      301 gcccatggcc atgcctcgat attgggaaaa atagaacggt gcagccaagg cgttatcgag
      361 gtcccgtttt tggccgccgc cgttctgcgg cttgcggtcc agctcaccgg cacggatgaa
      421 acggcactcc gttatccgga gcctgaccat gatcatatcc tgcttgcagg ttccaaactg
      481 ccgtgcttga gcgtgcggca agatgccgcg cccgcggctc tctcactcgc gatattggct
      541 gaccttcgct tacggaggat aatcttctag gctttgtcaa tgcagcctga acttccccgc
      601 actcggcgct tctttgggtt aaatccgccc gatcgcgcaa ttgggcatcg agtcccgtat
      661 ttgaagcgaa ccaaacaaat tgttcggcaa ggcgcctctt ttcgcttgcc aattccattg
      721 cgccgcacaa aatgccgcag cgaaactggc tcagttccgt gtctcgggag gtcataaatg
      781 aggtctgcga cctgtggact ttagtctctg ccctgacgcg gcggccttcg cgaaaggcac
      841 tggatgtagg tttcatttgg ccttggtatt caagcggctc accccagtta aattgcgggg
      901 caggggtgcg gcgagccgcg ccgactgtcc ggcgaggatc cttttcttgc ggagttcctt
      961 ctgccatgcc ggtcaggtgc aaagttccca aactggcagg tatcctcgat gattggaatc
     1021 atccttgtaa cccatgggtg ccttgcggcg gagttccgcg ccgcgctgga acatgtcgtc
     1081 ggcccgcaaa accagatcga gtccatcgcc attggtcctg aggacgatat cgagcaaagg
     1141 cgccaagata tcgttgcagc ggttgccaaa gtcgacagcg gcgctggtgt catcgtgctc
     1201 acggatatgt tcggcggcac accttccaat ctggcgattt cgattatgaa tggcgccaat
     1261 attgaagtca tcgccgggat caatctgccg atgctcatca aactcgcctc ggtgcgcgac
     1321 acagcgagtc tcgaacaggc ggtgattcag gcacaggatg caggccgcaa atatatttat
     1381 gtcgcgagcc gtgtcttaag cggaaaatga tcaaagatga tgccgtcccg gacgagccga
     1441 gcgattgtcc ggatcttgaa ccgccgggag acgtactcgt tcggaccttg gaaatcgtca
     1501 atcgcaaggg ccttcatgca cgtgcgacag ctaaactcgt gcaatgtatc gatggcttcg
     1561 atgctgtggt cacaatcagc cgctgcggag agacggttgg cggcagttcg atcatgggaa
     1621 ttttgaccct tggcgccggg caaggcatga ccatcaccgt taccgctgcc ggtcctgggg
     1681 cggctcaagc gatgaaagcc atcgccgcgc tcattgccaa ccgcttcggc gaggacgaat
     1741 aaccgcgaca gcaataacgc gcgctgtcgt tgctcaaccc tcgcgaaatg cgcgcgcggc
     1801 cgcgataagc cctaaggtgg atgcatcgat gcccggtccc gccgggcttc ggccgtcgac
     1861 gataggtgtg agattgttgg cgagctcctt gccgagctca acgccccatt ggtcgaagga
     1921 gttaatgttc cacaccacgc cctgcacgaa aaccttatgt tcataaagag caatcaattg
     1981 cccgagtacg cgcggcgaca ttctgcgata gagaaatgtg ctggatggcc ggttgccttc
     2041 aaacaccttg tgtggcgcaa gcgcttcgat atctctgggg ctcacattct gcgctgctag
     2101 cccttccctg gcctctgcga tggatcgccc cttcatgagg gcttggctct gcgccaagca
     2161 atttgcaagc agcaattggt ggtgctcgac atcggcatcc aaaggatttg ccgcaacgaa
     2221 aaagtcgatg ggtacaacgc ttgtaccctg atgcaggagc tggaaaaagg catgctggcc
     2281 attcgtgcca ggctccccga atatgatggg cgcggtctca aagtccacgc gggagccgga
     2341 taggtctacg gatttgccat tggattccat ttcgagctgc tggagatagg ccggaaatct
     2401 tgccagccgc tcgtcgtagg ggatgacagc atgggtcgaa tagccccaga tgttgcggta
     2461 ccagatgccg agaagggcca tcagaaccgg aatattacgc tcgagcggtg ccttcgtaaa
     2521 atggatgtca atgtcttgcc cgccggccaa aaactcctcg aatttttcct tgccaatccc
     2581 gattgcgagc aagagtccga ttgatgacca gatggaataa cgcccgccga cccaatccca
     2641 aaagccgaac acccggtcgc tgcgaatgcc aaaggcggca attttgtcga gctgtgtaga
     2701 aacggcgcaa aaatgatcgc caactgccat ctcgccgagc cttgccgcaa cggcttggcg
     2761 cgcggaccgg gcattggtca tcgtctcgat cgttgtaatg tctttgagca gacgatgaaa
     2821 agtgtggttt cgagcggtac gagcttcaag gtgtcgccaa gatccgctcc atcggcgttc
     2881 gccacagtat gcagcctcaa attccgcgga acaaagggcg caagggcccg gacagccatc
     2941 gcgggaccga ggtccgagcc tccaatgcca atattcacga tatcggtgaa aggcgcgccg
     3001 ctcgtggatt tgacattgcc attgcggacc gcttctgcaa aagcctccat cctggctcgc
     3061 tcggccagaa cctccggcat gacgtctcgc ccagcggcga acatcgggtt gcccgagaga
     3121 ttgcgcaggg ccatatgcag ggctgggcgg tgttccgtgg cattgaccgg ctcacccgaa
     3181 aatagtgccg cccgacgcgc tccgacatcc gcctcgcgcg caagatctat aagaaggccc
     3241 agcgtcgttt gatttatttt gtgtttcgaa aagtcgaaca aaagcccatc ggcttcgaca
     3301 tggaactgcg agaatctccc aggatcatcg cgaaagaaat ccgcgatcgt ttgtctgcca
     3361 acggcgagtt tgtgcgcttc cagcgcggcg agggaatttg ctccttgctc acgagccata
     3421 agccgtctta atatactatc ggcaacgtca aaaattacaa ttgtgcgaca tgaggcgtgc
     3481 atagaccttg gcccgctgaa aacgggccat ggtctctttt cgtgtttagc gacaaaaccg
     3541 gcttgccggg gcgaagccaa tgccccggtc aggccggaag cttgtcactg gttataggcg
     3601 cgctcgccat gatcggtaat gtcgaggcct tcgagctcgg cctgcttcgt tgccctgagg
     3661 ccaatgatca ggtcgacgaa cttgtagagg atcgccgagc cgaaacccga ccagaatagg
     3721 gtgaaaagca cgttcttcgt ctggatgatc acctgtggca acatgctgta atccccggcc
     3781 gaggcaaagc caggtttgat cgtgtagtcg gtgaggcccg tgccgccgag gtagggcgcg
     3841 accaggatac cggtacctat cgccc
//
LOCUS       DATMPU010000319         3227 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_1189219,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000319 DATMPU010000000
VERSION     DATMPU010000319.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 3227)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3227)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3227
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1189219"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..117
                     /locus_tag="VNY06_07295"
     CDS             <1..117
                     /locus_tag="VNY06_07295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011217545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HWX14640.1"
                     /translation="YWGRHFWARGYFCTTSGNITDDVILQYIRSHEPTGASR"
     gene            492..1238
                     /locus_tag="VNY06_07300"
     CDS             492..1238
                     /locus_tag="VNY06_07300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX14641.1"
                     /translation="MIAVTPLLHEIIPHGWTRLALLETDATISRGYSENPAAAAVFRE
                     HMWRFTSDRSSPMSLWTSCFHSVGIGWTLHLQGRDWQTKAWYREIESPLDSCWILDAM
                     IGETGQASALVSLTRPRSARPFTVDDVQRLDRLRPWLAHAFRPEASDCAHIAGACLGD
                     DALMCAAGAPARSGQMILTGDEKIIFQTSDLEFLLRVLANEPGDHTRPSPPREKLPAP
                     IQLLIRRIVGAANGVSNTPPVPRFRLPTAF"
     gene            1259..1681
                     /locus_tag="VNY06_07305"
     CDS             1259..1681
                     /locus_tag="VNY06_07305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX14642.1"
                     /translation="MPAGAILEDVAKDPKSCLVAVTIELREHAVAHAARILRECGATP
                     AQLKVGIQLVLGKTRPTIADELGLKHSTVADLAKKLYQILDVRNATELGARIWLGKGR
                     KEVQRSELPRPPGFVASGARKAHIASLFSPSYKSLTHP"
     gene            complement(1819..2028)
                     /locus_tag="VNY06_07310"
     CDS             complement(1819..2028)
                     /locus_tag="VNY06_07310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX14643.1"
                     /translation="MSFVRAEADYLAAKNNGVSLTHVHDIEDQLFQQGTAQGITFVAS
                     NADHGAIPLVRGKPTVSARYPESDP"
     gene            complement(2411..>3227)
                     /locus_tag="VNY06_07315"
     CDS             complement(2411..>3227)
                     /locus_tag="VNY06_07315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020173600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="short-chain fatty acyl-CoA regulator family
                     protein"
                     /protein_id="HWX14644.1"
                     /translation="MSDRLRWYDHHRRQLMISEAVEPPGRIFQAAYQLALSQYGDLLD
                     EIAARLEPNHELTRRLLRVTLANYFAGAVMMPYGRFLEAAEVTGYDIELLAVRFGASF
                     EQVAHRMTTLARQTARGVPFFLIRVDIAGNVSKRFSSSRFPFAHFGGTCALWNIHAAF
                     AEPGRILTQTIELPDGTQWFSIARTVRRSLTPFGAMEPRFAIGLGCEIKYARRLVYAK
                     RLDFNALDATPIGINCRLCDRAVCPQRAAPPAMRPLHVDETTRSISPFTFRDV"
BASE COUNT          693 a          921 c          947 g          666 t
ORIGIN      
        1 tattggggcc gtcacttctg ggccagagga tacttctgca ccaccagcgg caacatcacg
       61 gacgatgtca tacttcagta tattcgttcg catgaaccta ccggcgccag ccggtagtta
      121 ttcagtggcc ccggattacc cgggcgatgg acaatttggc cgtccatcgt gtcgaccacc
      181 ggaaagtgca gctgccaaag gagttggggc caccagggcg gtgccgttga tggatccttc
      241 cacgcctccg ggccaggcgg caagggctgc gggtttaact ctgtcatgat gtcgacctct
      301 tcgatttcgg cggcgatcgg ccgccgggtt acgtgttgat ttgagcaaaa tttttggccc
      361 ccagcggccc ctgttcaggg gacgcatttg ggaatattac taaactaata atgacgcggc
      421 ggtttcaggg gggaagccgg tgggagcggt tgagcatcta aaaaccttgt gctgtcttgg
      481 gctcgagcgc gatgatcgcg gttacgccac tgctgcatga gatcatcccg cacggctgga
      541 cccgtctggc tctcttggaa accgatgcca cgatcagccg cggttattcc gaaaaccctg
      601 cggccgcggc ggtcttccgc gagcatatgt ggcggttcac gagcgaccga tcgagcccca
      661 tgtccctgtg gacgtcctgc tttcattcgg tcgggatcgg ctggactttg catttgcaag
      721 gccgggactg gcaaacgaaa gcctggtacc gggagattga gagcccgctc gattcctgct
      781 ggatcctcga tgcgatgatt ggtgagaccg gccaggcttc cgcacttgtc tcgctgaccc
      841 gtccgcgcag cgcgcgtcca ttcacggtcg acgatgtgca acgtctcgac cggctgcgtc
      901 cctggctcgc gcatgccttt cgccctgaag cgtctgattg cgcccacatc gcgggcgcct
      961 gcttggggga cgacgctttg atgtgcgccg ccggagcgcc agcacggagc ggtcagatga
     1021 tcttgacagg ggatgaaaag atcatttttc agacgtcgga cctcgaattc ctgctccgcg
     1081 ttctggcgaa tgagccgggt gaccatacga ggccatcccc tccgcgcgag aagctgcccg
     1141 cgccaatcca attgcttatc cgtcgcatcg tgggtgccgc aaatggcgta tccaacacgc
     1201 cccccgtacc cagatttcga ctccctacgg ccttttgacg cttgaggcca aatggctaat
     1261 gccagccggc gcgatactag aagatgtcgc caaggatcca aagagctgcc ttgttgcggt
     1321 aacgatcgaa ttgcgcgaac atgcggttgc gcatgccgcg cggatcctgc gcgagtgcgg
     1381 cgccacaccc gcgcaattaa aggtgggcat tcagctcgtc ttgggtaaga cgaggccgac
     1441 aatcgcggat gaacttggcc taaagcactc caccgttgcg gatctggcaa aaaagcttta
     1501 tcagattctc gatgttcgca atgcgaccga gctcggcgca agaatttggc tgggcaaggg
     1561 ccgcaaggaa gtacaaagat ctgagttgcc gcggcccccg ggctttgtgg catctggggc
     1621 aagaaaagcc catattgcct ccctattcag cccttcctac aaatccctca cccatcctta
     1681 aaagtgaaag gcgagaggct gcgcgtggct tcatcaacat acggcgggcg catcgcgggc
     1741 ggcgcgtaac gcgctccaaa tggttgtttg atcaaggcgc gttccggcta tggttactta
     1801 agtgtaaacc gcgaccactt atgggtcgct ctccggatat cgggcgctga ccgtcggctt
     1861 acccctgacg agtggtatcg cgccatgatc tgcgttgctc gcgacaaagg tgatgccctg
     1921 ggccgttcct tgctgaaata gctgatcttc gatgtcgtgg acgtgggtta gcgaaacccc
     1981 gttgtttttg gccgcgagat aatcggcttc cgcccggacg aacgacatgt tcacaacgtc
     2041 cgctatgttg tcttcaacga tttggtgtag tccggaaatt tcgttacagg tatccaactt
     2101 ggggatgctg tacagcagga tgctgctgcc agagacatgc cacccgattg ctgcacatca
     2161 agagtagctt cactcgaagc cgttcgactg cctccgttga ctttaactat tttgaacgat
     2221 ggccgaagga gagctggcag gccaaaggcc gtccagtatg ccgtaaggaa caggaattga
     2281 cgacgcggta tgataaacga tcaaaagcga cacatccggt tcctgtatcg attgcaggcc
     2341 tgcgcgaagt ttgaactgcg gcgtgataga aaaagcaagt ggcgacgctt gttgaattta
     2401 tagccaaacc tcacacgtcc ctaaacgtga aaggcgagat gctgcgcgtg gtttcatcaa
     2461 catgcagcgg gcgcatggca ggcggcgccg cgcgctgtgg acagacggcg cggtcgcaga
     2521 gccggcagtt gatgccaatc ggggtcgcat ccaacgcatt aaagtcaagc cgcttcgcat
     2581 agacaaggcg ccttgcgtat ttgatttcgc agccgagccc gatcgcaaac cggggctcca
     2641 ttgcgccaaa aggggtgagc gagcggcgca ccgtgcgcgc aatcgaaaac cattgcgtac
     2701 catcagggag ctcgatggtt tgggtcaaaa tccggccagg ctcggcgaaa gccgcgtgaa
     2761 tattccacaa ggcgcaggtg ccaccaaaat gggcaaaggg aaagcggctt gacgagaacc
     2821 gtttcgagac atttccagca atatccacgc gaatgaggaa aaagggcacg ccccgcgctg
     2881 tttgccgcgc gagcgttgtc atgcgatgcg ccacctgctc gaaactcgcc ccgaaacgca
     2941 cggccagaag ctcgatatca taacccgtaa cctcggccgc ctctaaaaag cggccataag
     3001 gcatcatcac cgcgcccgca aaataatttg caagggtgac acggagcaat cgccgggtta
     3061 actcatgatt cggttcgagc cgagccgcga tttcgtcgag gagatcccca tattgactta
     3121 atgcgagctg ataggcagcc tgaaaaatcc ggcccggcgg ctcgacggct tccgagatca
     3181 tcaattggcg gcggtgatga tcataccagc gcaaacgatc gctcatc
//
LOCUS       DATMPU010000320         4008 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_1190669,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000320 DATMPU010000000
VERSION     DATMPU010000320.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 4008)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4008)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4008
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1190669"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(205..510)
                     /locus_tag="VNY06_07320"
     CDS             complement(205..510)
                     /locus_tag="VNY06_07320"
                     /inference="COORDINATES: protein motif:HMM:NF018151.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HdeA/HdeB family chaperone"
                     /protein_id="HWX14645.1"
                     /translation="MRADLASLGLVITLLSASLAHGQVNIDVSKVTCDQFAQGKIGLP
                     RTTAIWLNGYYHGKNGSTTIDTQQSEAIFGELMDFCRTGENGKILVMQALEHLMKRK"
     gene            complement(1821..1997)
                     /locus_tag="VNY06_07325"
     CDS             complement(1821..1997)
                     /locus_tag="VNY06_07325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX14646.1"
                     /translation="MSALSRRTIVTSAAALPALAVPAVAACAEPDPIYAAIEKYRIAE
                     DAFLARANYEDGQN"
     gene            complement(2190..2669)
                     /locus_tag="VNY06_07330"
     CDS             complement(2190..2669)
                     /locus_tag="VNY06_07330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX14647.1"
                     /translation="MTYLSQRTEAGHFDRFAQWLITNLLVYVGGAILFGVGFLAFAAL
                     YVYFVPKPSVIPTQSPAPVQIAPKSEAPSFQLPPKPTDKLGPIAKPQLALPIPVANLA
                     DRDLLGKKLVDPQGVSLGYIATVNRDAKGMITSLGLQPAQNLGPNAPVLDVKIAPAQ"
BASE COUNT          906 a         1111 c         1137 g          854 t
ORIGIN      
        1 gattgggctt gtagccggca gcacgataag cttcggtcat cgatttgcct tcggcgaggc
       61 cctgagcaaa aagctcgtgt ttcgtattgc gtagcggggc cattagtcag ttccgcagtt
      121 gaggaaatgc attatagatc gacggcttaa cggcactcca ggaagcgcct tgcgcgccgt
      181 attccacaag tcgtaaccga ctagctattt tcgtttcatg aggtgctcca gcgcctgcat
      241 gacgagtatt ttgccgtttt cccccgtgcg gcaaaaatcc ataagctccc cgaaaatagc
      301 ttcagactgc tgcgtatcga tggtcgtgct accattcttg ccatgataat aaccgttcag
      361 ccaaatagcc gtagttctgg gcagaccgat cttgccttgt gcaaattgat cacaagttac
      421 ttttgaaaca tcgatgttga cctgcccatg ggcaagcgaa gcagataata gagttataac
      481 gagccccaac gaggcgagat cggcacgcat aaacaacctc cattgatttt gcagtgcgca
      541 tcataccggg cgctgcgagt ggcctcatcc tcgaaataga acgattttgg atgcagctct
      601 ccaacaaaat tgtatagttg ggatcatcaa aggagtgtgc tcgatgcgct tctttatcat
      661 cgtcatcgcg atgtcattgt ctaccttggc atctggctcc acggcaatcg ctcaaaaggg
      721 acaacccgca ccagccctgc cgacacagga ccaagccgcc gccgaaaaac agaatccagg
      781 ctggtttcag gagacggcgc cctacagacc ttgcccgacc gtggctgcaa tcaatggccg
      841 caacgtttgt ctgggctgcc cgggacggtg tccatggcct ccatcgtttg gaaagtagtt
      901 ggcgggatcg gcagataacc agccagaggg gaccggggct aagtcagtgc tgtagtgcta
      961 cgttgcatcg cctattcagg ccgaatgccc agcgggagtc aattggaacg aaagagcagc
     1021 aagaatcttg gcctagtgtg gccagttcgg aaagcatcgc ccccgcgaag cctacaacac
     1081 tggcacacag cggcctcggt ggtttcccaa agctgcgccg gggccgccaa ataaaaaaac
     1141 cccagccaga tcggatactg actggggcta agtcaaggac cgttcgcaag cgttggcacc
     1201 tggagtagcc agcgctcacg gccgcaattc aggccggtga acctttgcga gtcaatgaca
     1261 gccacgcaac acttcacata aaaagcccca gccagtttcc gcttctgacc ggggtgctag
     1321 tgtgcccctg aggggctcgg ctattcaggc cgaatgctcg gcgggagtca aattaggtct
     1381 tggcgaactt ggccgtcgcg gtgccgggtg tcgcgatgaa tttgcctccc gacggtcctg
     1441 atcgtgcacg tacaggttca gatctgacgc ctggttggcc gttcataaca gccgcgccag
     1501 cccgtagccc aacacgccga cccacaagcc cgcgccccgg cgaacaaatg actatatttg
     1561 caaatagtcg cggcctctgc cgcgcccgat catcatcccc gacgttatgg agctgacgac
     1621 gctcgccgat gttcggaccc tggtcgagaa gcacctgccg gcggaatacc gttcgaagtt
     1681 cagctggcta gccggccgga ccgctccgac gcgtggccca aggccagcag gacatcggca
     1741 agcgcccgcc gcgcatctac cgatgctttc accgcagcga ccatttcggg gggtctcgta
     1801 aatttctccg ggcgccggca ttagttttgt ccatcctcgt aattggctct cgccagaaat
     1861 gcgtcctcag cgatgcggta tttctcaatc gctgcgtaga tcggatccgg ctcagcgcat
     1921 gcggcaaccg ccggcacagc caaggctggc aatgcggcgg cgctggtcac gatggtacgt
     1981 cgggatagtg cagacatggc aattcctttc ctggtgtgtg tgaaagcacc ggccaaggcg
     2041 gaatgacgct cagtattttt gtggtaggaa tcgtgcgggt catggcgcgt ccgtgcctgg
     2101 tgcgctgtga tcaagtcgcg gtcgggtgtg tgacagcacc cggccgcggc gcttattgga
     2161 ctctgatccg gttaaaaaag caattccgtt tattgcgccg gtgcaatttt gacatcaagg
     2221 acaggggcat tcggtcccaa attctgagct ggctgtagtc ccaaagacgt gatcatcccc
     2281 ttagcgtctc tgttcaccgt tgcgatatac cccaacgaaa cgccctgcgg atcaacgagt
     2341 ttttttccta gtaaatcccg atcggcgagg ttggccaccg gaatggggag tgccagttgt
     2401 ggtttggcaa tcggtccgag tttgtctgta ggtttaggcg gtagttggaa ggatggcgcc
     2461 tcagatttag gcgcaatttg aaccggcgct ggagattgcg ttggaatgac cgacggtttg
     2521 gggacaaagt atacatagag cgctgcgaac gccaaaaacc cgactccaaa aagaatcgcg
     2581 ccgcccacgt acaccagcaa attcgtaatc agccattgag cgaagcggtc gaagtggccc
     2641 gcttctgtac gctggctaag gtaagtcacg attgcctccc taactcacgc caagctgccc
     2701 ttaatccctg tacaaaaaaa accacaagca cgccaagatc catagtctga aaaatgtatt
     2761 caaagggaaa acgtccaaaa accaatggaa cgcgatctcc atatagatag tgcgtccacc
     2821 aatctccgag ccagatgacg gtaataagac ctatcgataa aagcacatgg ccgcaaagaa
     2881 cgacggccgc ccagacgggc cattcaagcg gcccgagata tgcccaccaa cccctcatcc
     2941 aatgattgta tgtgaataat agcacaacgc gctgtttggc ttagccggct aagttcttga
     3001 ttttgtttgg ctccccttcc tggatacgta tcgaactatg tgcctcgccc cggagcctcc
     3061 gttccggcgt gttttggagg aaattagggc ttggagctaa gttccacact acggtgttcg
     3121 acgacgagga aggcagtgca ggggcagtgc agggggcagg ccagggcgtt tacccttgag
     3181 aactgctcaa cgacgcaacc gcttcacgga tcgcgttgcg ccccgtcgcc ctttgactac
     3241 cctcaaaaag gcgaaagact taggagacga gtttatgtcc accacgcctg cgggttctcg
     3301 atcttcagcc gcacggacaa ggcgtcatcg ggagagacgg cggcagcgaa cgcgttgcgt
     3361 tacggtggac gtcaaccaaa gcgagcgcga tgccttagtc gtcaggggct atttgtccga
     3421 ggaagatcgg gacaacggcg ccgcaattaa aaaggcgatt gagggcgtaa tctcagacat
     3481 cgtgttcgag cttcaatccg aagccgctga gcgctgtcga gctcgcgttt gatcgataga
     3541 tccgacgaaa cgcgtcacag gtcggagagc aaattccacg cgttatgcgt cactgctgcg
     3601 acaacggctg cactccggca cggacacgcg tcacctggtg ctgctgccgt tgcgtcagaa
     3661 cctttcgttg acctccacaa gggaggcatt ggcggcggtt tgggtacgaa tgctcgattc
     3721 gtggatgatg agaagaagtt ccgcttctgc ccatatcgca ttgagtagac acggttttca
     3781 gcgatagcat tatttttcag gggtgcttgg atttcggcac cgccgcccgg agttggtctt
     3841 tttggccggg gtctagttgc attgtttaag tgggttgagg tgtccgacgc tggagaagcc
     3901 gccttccgaa agcgcgacgc agaggaccca aagcctgtgt agcgagggag agaacgatgg
     3961 aacggctcaa ggcaagcgca gactgttggc tgtccctcgc ggctgcgc
//
LOCUS       DATMPU010000321         5108 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_1507593,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000321 DATMPU010000000
VERSION     DATMPU010000321.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 5108)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5108)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5108
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1507593"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            158..496
                     /locus_tag="VNY06_07335"
     CDS             158..496
                     /locus_tag="VNY06_07335"
                     /inference="COORDINATES: protein motif:HMM:NF012680.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KOW motif-containing protein"
                     /protein_id="HWX14648.1"
                     /translation="MITVSGLRFREGARVKVLAGPHAGKSGIVERLLLNHLHAYVIQP
                     HEGEAFQAADDQVQAIAGWSPSVGWWPLGVTSGMRRRLKKEKAHALPSLAVNVSMPLP
                     PLKLRPLPCP"
     gene            complement(1397..1612)
                     /locus_tag="VNY06_07340"
     CDS             complement(1397..1612)
                     /locus_tag="VNY06_07340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013504282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HWX14649.1"
                     /translation="MGSTTDKVSGVANQAAGKVKEAAGKATDSEKLEAEGLAQQAKGK
                     AEKLAGDTKEAVKDTTKKVADAANKRL"
     gene            complement(1736..2794)
                     /locus_tag="VNY06_07345"
     CDS             complement(1736..2794)
                     /locus_tag="VNY06_07345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014892909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc-binding peptidase"
                     /protein_id="HWX14650.1"
                     /translation="MKLFLCQHCQQTLLFENTKCERCGHQLGYLPDQGVLSAVEPSGA
                     NWIALASPNQTYRFCANWEHEGCNWMVTPDEHDALCTACRHNRTIPDISNPIHRSLWQ
                     KIEAAKRRLIYTLINLSLPLPTAASGDGEPLIFDFLANPPDTSGAPKVMTGHDNGIIT
                     LSLVEADDAIREQLRVSMGEPYRTLLGHFRHECGHFYWDKLVRDGGKIDSCRAVFGDE
                     SEDYEDALKRHYANGAPPSWQQEFVSAYATSHPWEDFAETFAHYLHIIDTLETARAFG
                     LRTRPAVPDGAVLRAKIDFDPHRCRDINQITDVWLPLTFAMNCINRSMGQPDLYPFVL
                     SPSVIGKLGYIGNLIHAA"
     gene            complement(2791..2988)
                     /locus_tag="VNY06_07350"
     CDS             complement(2791..2988)
                     /locus_tag="VNY06_07350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX14651.1"
                     /translation="MGPGYRVYFGRDGKALVILLTGGTKQRQPRDMERAKALWADYKR
                     GRKPPVSPTVILTLGNAGATR"
     gene            3417..3734
                     /locus_tag="VNY06_07355"
     CDS             3417..3734
                     /locus_tag="VNY06_07355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX14652.1"
                     /translation="MPWDAAFRITKLTGGKVCFVLASSGHIAGIINPPDGKGTYWTNK
                     DGQADTPERWRADAQRHDGSWWTDWFAWLAAHSGEKVEPPSLGSEAYPPLQDAPGSDV
                     LEK"
     gene            4191..4475
                     /locus_tag="VNY06_07360"
     CDS             4191..4475
                     /locus_tag="VNY06_07360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX14653.1"
                     /translation="MTEAKKLIEKIPCPFVYANGKKCTGHVDRIEAYKADIMWSPNED
                     GTWKAHIEPRSHYHVFCSAKGNHAGYGREDSPKLKFYYRDLPKELQAALL"
     gene            complement(4673..5092)
                     /locus_tag="VNY06_07365"
     CDS             complement(4673..5092)
                     /locus_tag="VNY06_07365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008135949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX14654.1"
                     /translation="MPVSLKDILEAFEFVCADGGDEHQAFLCKQSGKLYCHSELWDNS
                     DKLPDDIGDREKFLQIPDKRELDLGKPLALDFARHFLPGDFDDVLQFFRGRGAYARFK
                     DLLGGRGMLDQWYDFEAKAVERALKMWCDLNSIEVSN"
BASE COUNT         1108 a         1477 c         1411 g         1112 t
ORIGIN      
        1 aattctccct ctactcgtaa ctgaagcgag cgtagctctg tgcggctccg atgccttcca
       61 tgctcaatcg aagcggaagc tcgccattgc accaccagct aacggtagct gcttgttcaa
      121 atcggcgtga gcaagatggg actcattagg ctgcacgatg ataaccgttt cgggtcttcg
      181 gtttcgagag ggagcacgag tgaaggtctt agctggtccg cacgcgggca aatccggcat
      241 cgtcgaaagg ctgctcctta atcacctcca cgcctacgtg atccagccac acgaaggcga
      301 ggcttttcag gccgccgacg atcaggtcca ggccatagca ggctggtcgc caagcgtcgg
      361 gtggtggccc cttggcgtca cgtccggcat gcgccgccga ctgaaaaagg aaaaagcaca
      421 cgcgctaccc tcgttggccg ttaacgtgtc aatgcccctc ccgccgctca aactcaggcc
      481 gctgccctgc ccctgatacc attcaaagcc gccggtctgg attgcgtaat gtaccttcgc
      541 gccgcggtgt cggtggtaca atagggcctc gccgcagcat ctagctcctc gccagcttcc
      601 cgttaataat tggcgagtaa aggttggagc aatgaggcaa accaatgatc cattcatcgc
      661 ggcgggttgg caggaccaaa gcaatcgccg aagtgctggc aatgatcgcg gacgcacacc
      721 ctgagattgc accacaccat cctcgcccgg ttcacgagtc aggcgaatgg ttatgggaga
      781 gcgccacaga attttgtact gcactagacc gctatgcaga gcaagccgcg cgactgaatc
      841 gaaaattgct gagcgctccg gcttcttgtt catcgccgaa agtggtggag ggcgagtcat
      901 acggttgtcc tcggccggta ctgcccgcgc cgtggatttc aaagcgtcga atgacaatta
      961 agagacgcgc aggcgctcag agccgcgaga ggctacatcg agcttttttc gcgcttccct
     1021 gatagcagcc ctcttgcttg ccgccctcgc cagaacaagc gaaacccagc gcttcagctc
     1081 gacgctgggc atttggatag tgatgatctt gccgcatgat cggatagcat catgccgccc
     1141 ggagccacgt cattaaaatc cgcgacaggc acccccttga gtaccgccta aaaccagcgt
     1201 ccccgaccgt accagccgcc tccgcctagg aggagaacaa gaacaataat gatgagaagt
     1261 gtctgggtat ccatgatgtc caaccctttc ggtacgatgg tttttcaggt gtactgcaag
     1321 cgcgccaagg agaagcaaaa ttaaaatgat gagcaggata gtgccaagcg gcggtcaacc
     1381 tccctcacac agcaattcag agtcttttgt tcgcggcatc ggcaactttt ttggttgtgt
     1441 ccttaaccgc ctctttcgtg tcaccggcga gcttttcagc cttgcctttc gcctgctgag
     1501 cgaggccttc ggcttccagc ttttcagagt cggtggcttt accggctgct tccttgactt
     1561 tacccgcggc ctgattggcg acgcctgata ccttgtctgt ggtgctaccc atgatccata
     1621 tcctctcgtg ggtgtttgtc atgattgacg acagaagaac gaatgcccgc caataaggtt
     1681 ccgccgattg actgcacgca atcgggttca cgccatgcgc cgagcgaaag cgttttcatg
     1741 cggcatggat caaattcccg atatagccaa gtttcccgat gacgctcggc gatagcacga
     1801 atggataaag atcgggctgc cccattgatc gattgatgca gttcatcgca aaggtgagcg
     1861 gaagccagac atctgtaatt tgattgatat cacgacagcg atgcggatcg aaatcgattt
     1921 tagcgcggag cacagcaccg tcgggaaccg caggcctcgt acggagtcca aaagcgcgag
     1981 cggtctccaa tgtatcgata atgtggaggt aatgcgcgaa ggtttcggcg aaatcttccc
     2041 atggatgcga ggtcgcatag gcgcttacga attcttgttg ccaagaaggg ggtgcgccat
     2101 tcgcatagtg gcgcttaaga gcatcctcgt aatcctcact ctcgtcgccg aatacggctc
     2161 ggcagctgtc gatcttgccg ccatctcgca ccaacttgtc ccagtaaaag tgaccgcatt
     2221 catggcggaa atggccaagc agtgtgcggt agggttcgcc catggacact ctaagttgct
     2281 cccgaatcgc atcatcggct tcgacgagag ataaagtaat gatgccatta tcatgccccg
     2341 tcatgacttt tggcgctccc gaggtgtcgg gtggatttgc tagaaagtcg aagataagcg
     2401 gttcaccgtc gccgctggcg gccgtgggca aaggaagact gagattgatc agtgtgtaaa
     2461 taagccgtcg tttcgcagct tcgatttttt gccagagtga acgatgtatg gggttggaaa
     2521 tgtcggggat cgtgcggttg tgccggcagg cggtgcagag agcgtcatgc tcatccggtg
     2581 taaccatcca gttacagcct tcgtgttccc aattggcgca aaatcgatag gtctgattgg
     2641 gggaggcgag cgctatccaa ttggcgccgc taggctccac ggcgctcagt acgccttgat
     2701 ccggaaggta gccaagttgg tgaccgcacc gttcgcattt ggtgttttcg aagagaagag
     2761 tttgttggca atgctgacag agaaaaagtt tcaccgcgta gctcctgcgt tgcccaatgt
     2821 gagaatgact gtgggtgaca ctggcggctt ccgcccccgc ttgtaatctg cccacagcgc
     2881 tttggcccgc tccatgtcgc gcggctggcg ctgcttggtg ccgcctgtca gcaggatcac
     2941 cagggccttg ccgtcgcgcc caaaataaac ccgatacccc ggccccaatt gatccggtaa
     3001 cgcgacagtc tataggaacg gaacagatga aaggatgaca gcctcgcgcg gtccaggtct
     3061 cctgcgtcaa ggctttccgt cacatggaag ggcggcgatc acctttcgag actgccggag
     3121 ggaaaccaaa tagcgctcct tcacggcctc tttagtccat gtttataagc tccgttcgcc
     3181 ttgcccttcg gcgctccgca acgcgcaacg tggaagcggc acccggtcca accccgcacc
     3241 gtaggggcct tgcaccgctc gcgctcgccg agcaccgcgg agctttttgg aaagaatact
     3301 cccgtttcat gaggatggct ccgttttctc cgaaaaggtg cgggcgagct tattcgaggc
     3361 acgctccgcg ctgtcctgtt gcgatctacg cggttggggc ggacaaggac cacatcgtgc
     3421 catgggacgc ggcctttcgc atcaccaaac tcacgggcgg caaggtgtgc ttcgtcctcg
     3481 cgtcgagcgg ccacatcgcc ggcatcatca atccgcctga tggcaaaggc acctattgga
     3541 cgaacaagga cggccaggcc gacacgcccg agcgatggcg cgcggacgcc cagcgccacg
     3601 acgggagctg gtggacggac tggttcgcct ggctcgccgc gcactccggc gaaaaggtcg
     3661 agccaccgtc cttgggtagc gaggcatacc cgcccctgca ggatgcaccc gggagtgacg
     3721 tgttagagaa gtagcccccc gccggcggtt ggcgacaggc agggaaaact gaccgctaca
     3781 gaaatcgccg ccgcagattc agcctgcgct tcttcctcat cgccgcaatc taacaatatc
     3841 gaactcaaaa ccgcataaag gttatgcaag aggtccaatg agggcgcgga tcgctgacga
     3901 gcgactcatt ccgtcgaatt gcccggccca gcgttcgaca gcttccagca actgccgagg
     3961 cagcctgacg ggaaccaatg gatcgcgccc ttttgctggt ctgccggggc ttttttgtga
     4021 taccgttttc atattttcgg tgacaccgtt tctatacttt cggtgatacc gatttatctt
     4081 tttggccgtc aatttgccag ctcccgattg atcagcttgc tcagcccgcc ggcaacagat
     4141 cgtcgagatc atcgtttggc cccggcccca tccccagcga ttggttggct atgacggagg
     4201 caaaaaagtt gatcgaaaaa atcccctgtc catttgttta tgcaaatggc aaaaaatgta
     4261 caggccacgt cgatagaatc gaggcctaca aagcggacat aatgtggtct ccgaacgaag
     4321 acgggacatg gaaggctcac atcgaaccgc gctcacatta ccacgtgttc tgttctgcga
     4381 agggcaacca cgccggttac ggccgcgagg actcaccgaa gttgaagttt tattatcgcg
     4441 atcttcccaa ggaattgcag gctgcgctat tatagtagac ccctcatctc cccaatgatc
     4501 gccgccgtca ccgcctggca ccgcgcgact tcggccagct cggcttggag tacgatcttt
     4561 acaacgacga cgaattggag agcgatccga cgtatcgaaa ctttgccgtc atgtcttcgc
     4621 cgtcaatcgg cttcatgtcc acgatatgag ctttaacaag gaggagccgc tattagttgc
     4681 tgacctctat tgaattaaga tcgcaccaca ttttaagtgc tctttccacg gccttcgcct
     4741 caaaatcgta ccattgatcg agcatgcccc ttccacccaa cagatccttg aatcgggcgt
     4801 aggcgcccct tcccctgaaa aattgtagaa cgtcgtcaaa gtcgccgggc aggaaatggc
     4861 gggcgaagtc caacgcgagc ggcttcccga gatcgagctc tctcttgtca ggtatttgga
     4921 ggaatttctc cctgtcgccg atgtcatccg gcaatttgtc cgagttatcc cagagctcgg
     4981 aatgacaata gagcttgccc gactgcttgc acagaaaggc ctgatgctcg tccccgccgt
     5041 cggcgcagac gaattcgaag gcttccagta tgtccttcaa gctgacaggc acggtgatcc
     5101 tcgccgat
//
LOCUS       DATMPU010000322         2745 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_3058181,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000322 DATMPU010000000
VERSION     DATMPU010000322.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 2745)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2745)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2745
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3058181"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(164..1864)
                     /locus_tag="VNY06_07370"
     CDS             complement(164..1864)
                     /locus_tag="VNY06_07370"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003613391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HWX14655.1"
                     /translation="MPPAAPPAVDSIATALGQTNAWPFEEARKLLRRVETTCQSEVIF
                     ETGYGPSGLPHIGTFGEVARTSMVRHAFEVLTGNRFKTRLIAFSDDMDGLRKVPDNIP
                     NKDLVAANLGKPLTQVPDPFGTHSSFGAHNNARLRAFLDQFGFDYEFLSATDYYLSGR
                     FDEALRHMLAKYHDVMAIMLPNFREERAASYSPFLPIHPRTGIVMQVPIDAVDTVAGT
                     IAWRDPTTHENYETKVTGGACKLQWKPDWAMRWYALKVDYEMYGKDLIESVKLSSAIV
                     RALGGNPPDGFSYELFLDERGQKISKSKGNGLTIEEWLTYASPESLSLFMYQKPTAAK
                     RLYFDVIPRTVDDYLTFLDAYPRQAAKKRLNNPAWHIHTGEPPLPEVLAHGTSRTTII
                     FGMLLNLAAVANSEDPQVLWGFLRRYAPDVSPATHPGLDHLVTYAVRYFRDFVLPAKR
                     YGEPDAVERESLEKLDAALAALPKGASAEEIQSLLYDVARKIPRYQDMQAKGAPAERP
                     GVSNDWFSMLYKILLGESRGPRLGSFVALYGIQETRELIAQALSGDLVRSQATRHAQK
                     "
     gene            complement(2076..2174)
                     /locus_tag="VNY06_07375"
                     /pseudo
     CDS             complement(2076..2174)
                     /locus_tag="VNY06_07375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611329.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            2210..2557
                     /locus_tag="VNY06_07380"
     CDS             2210..2557
                     /locus_tag="VNY06_07380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX14656.1"
                     /translation="MVPRSQLGAVMGFVADEALQCFACGDEDWRSTRLLWPAPRCGLA
                     GEELFLPLEGHLLKVEIGLHARELPRCHFESGLGLLHGRLSLLELRLQLRVLDHRQHL
                     PPFHMVADIIVDL"
BASE COUNT          606 a          730 c          800 g          609 t
ORIGIN      
        1 tatgaacgcc aagaagaggt ggcagcgtgc caccgtaaag ccgggttaat aatccctatt
       61 tcgaaaatgc gccgaacaat ccctgacgta gaggggttcc acaagaacca gaatatggag
      121 ttcgatcaaa acatcatcca ataaaaaagt taattcgagc tccttacttt tgggcgtgac
      181 gcgttgcttg gctgcgcacg aggtcgccgc ttaaggcttg ggcgataagc tcacgcgttt
      241 cttgtattcc ataaagtgct acgaaagacc ccaaacgcgg acctctgctc tcaccgagga
      301 ggattttgta aagcatcgag aaccaatcat tggagacgcc cggcctttca gccggcgcgc
      361 ccttggcttg catgtcctga tagcgaggta tttttcgggc gacatcatag agcagcgact
      421 gaatttcctc cgcgctcgcc cctttaggca gcgcagcaag agcggcgtcg agcttttcga
      481 gcgattcgcg ctcgacggcg tcgggctcgc catatctctt tgccggaagc acaaaatcgc
      541 ggaaatagcg gacggcataa gtcacaagat ggtcgagacc cggatgcgtc gcgggagaaa
      601 catccggtgc atagcggcgc agaaaacccc aaaggacttg tgggtcctcg ctgtttgcaa
      661 cggcggcgag attcaagagc atgccaaaaa taattgtcgt acggctcgtg ccatgagcca
      721 agacttcggg caagggaggc tcgcccgtat gtatatgcca ggccggattg ttgagccgct
      781 ttttggctgc ttgccttgga taggcatcga ggaaggtaag ataatcatcg accgtgcggg
      841 ggatcacgtc aaagtagagc cgcttggcag cggttggttt ctgatacatg aaaagtgaca
      901 ggctttcggg acttgcgtag gtgagccact cttcgattgt cagcccattg cctttcgatt
      961 ttgagatctt ttgccctctt tcgtcgagga acaattcata gctaaaccca tccggagggt
     1021 tgccgccaag tgcgcgaacg atggcgcttg atagtttgac cgattcgatc aaatcctttc
     1081 cgtacatctc atagtcaacc ttgagcgcat accaccgcat cgcccagtcc ggtttccatt
     1141 gcagcttgca ggcaccgcct gtgacctttg tttcataatt ttcgtgcgta gttggatcgc
     1201 gccatgcaat cgtgccggct acggtgtcga ccgcatcgat cggtacttgc ataacgatgc
     1261 cggttcttgg atggatcggc aaaaaaggag aataggaagc ggcgcgctct tctcgaaaat
     1321 ttggcagcat aatcgccatt acgtcgtggt acttggcaag catatggcga agcgcctcat
     1381 cgaagcgccc ggacagataa taatccgtgg ccgaaaggaa ttcgtaatcg aagccgaatt
     1441 gatcgagaaa ggcgcgcagc cgtgcattgt tatgcgcgcc gaagctcgaa tgagtgccga
     1501 aaggatcagg aacttgcgtc aacggcttgc ccaaattagc ggcaacaagg tccttgttag
     1561 gaatattgtc aggcaccttc cgaaggccgt ccatgtcgtc tgaaaatgca atgaggcggg
     1621 ttttgaaccg gtttccagtc aatacctcaa acgcatggcg caccatgctg gtgcgcgcga
     1681 cttcgccaaa agttccgata tggggcaggc cggaaggacc gtaaccggtt tcgaaaatga
     1741 cctcgctctg gcaagttgtc tcgacacgcc gcagaagctt ccgcgcttcc tcgaacggcc
     1801 aagcattggt ctggccgagc gcggtagcaa tggagtccac agccggaggt gcggcgggag
     1861 gcatggcgcg accttgagaa aatgcgaaaa tgtgcgggat cggttcagct ggtaagcatg
     1921 gctcgaacga taagaactac accgcgacca gtcaattccc gccttgttgg gccgcgtgca
     1981 ggattgggat gtgggacgcg ctcaatcgtt gggttagcga gattgtgccg cggaaagccg
     2041 tcagaaactg ccgaaggtct aagggttggg actcattatg tccgccatgg gagagctgca
     2101 gcgatgcagg cggaggccag gaagttacat gccaacctgt catggcgcgt gacgatacgc
     2161 ctgaatcgac cgaaagcgtt ctcctcgttc cctgtaaagg cgctttgctt tggttccgcg
     2221 atcgcaactc ggcgctgtca tgggctttgt cgccgatgag gcgttgcaat gctttgcctg
     2281 tggcgatgag gattggcgct cgacacgcct tctttggccc gcgcctcgtt gcggccttgc
     2341 cggcgaagag ctctttctgc cgcttgaggg tcacctgctg aaggttgaga tcggcctgca
     2401 tgcccgcgag ctgccgcgtt gccacttcga gagcggcctt ggcctgctgc acggccgcct
     2461 gagccttctc gaattgcgct tgcagctccg ggtcctcgat caccgccagc acctgccccc
     2521 gttccacatg gtcgccgata tcattgttga tttgtgagac gtagccagag taaagatccg
     2581 tgagaaaaaa cgcctgaatc gtcgccggga tgacaacagc cgccgctcgc tcctgtcttc
     2641 gcttgctggg tttggcgaag gtttgaccgt taccaactgc ttctgagcgg ctggtgagcc
     2701 taactgggcc tcggcggaat aaatgcaaat gggaacgggc gacac
//
LOCUS       DATMPU010000323         4510 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_4259873,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000323 DATMPU010000000
VERSION     DATMPU010000323.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 4510)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4510)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4510
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4259873"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..661)
                     /locus_tag="VNY06_07385"
     CDS             complement(<1..661)
                     /locus_tag="VNY06_07385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008136859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HWX14657.1"
                     /translation="MPDTIKRKPEDQTQLFEQVCKVERDVIERRRHFIKDKYGRERTG
                     SPVTGLALSGGGIRSASFGLGVLQALQAKHENHPVYGIEGIDYLSTVSGGGYIGSSLT
                     AALQKTECKFPFVNVDKPEDYADTSSVRHIRDYSNYLIPHGALDIVTALGIIGRGIVA
                     NWLIVAPVLLFFVWITLFIHPTVESLHQPKFLKWDLADALAKLGLPVSDPLWGLSGYW
                     FT"
     gene            complement(1302..3353)
                     /locus_tag="VNY06_07390"
     CDS             complement(1302..3353)
                     /locus_tag="VNY06_07390"
                     /inference="COORDINATES: protein motif:HMM:NF012935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HWX14658.1"
                     /translation="MIECDYLVVGSGAGGGTLAARLAEAGMRVVLLEAGGDPADPAKQ
                     AEKMPEDYAVPAFHPLASEHPAMSWKFYVRHYSDDERQRADPKCVPDPLTQRPSTYYP
                     RAATLGGCTAHNAMIFMAPHESDWDEIARITGDGSWSAQNMRRYLRRVEDCRYRPIWR
                     FFTRLYHDLTGQGWPPWLSTELALPRKTVHDSRLMELICETVSFVSRIIRRERMATLI
                     KRAGLTKIWEALEQAWKAVQLGLRSVVEKLALGALIPFVLEGANPNGQWCLRRRAEGS
                     CFVPLSTRGGQRGGTRERLLEVAARHPRLLQIECNALATKVLFDAENCAVGVEYRKGE
                     RLYRAHDDASAGEGEICTIRPAREVILAGGAFNTPQLLMLSGIGPKEDLEARGIRVVH
                     PLEGVGKNLQDRYEVGVVNWLDKDWNALKGAEYRRGDDPLFQEWLNRPKFRLLPARWR
                     KILPQSYQEKRRRGIYTSNGAAVAFTRRSSPGLPDPDLFCMALLGKFTGYYPDYSRKD
                     CCNPNNLNYVTWALLKAHTINRAGFVTLRSNDPREPPEINFRYFGEGDDLAGNDLDAV
                     VEGIRLVRKITARMKERGLKMTEKLPGEKLETSEELANYVRDAAWGHHASCTCPIGPP
                     RSGGVLTSDFKVHGTKRLRVVDASVFPRIPGFFIASAIYMIAEKAADVILAETKDARV
                     A"
     gene            complement(3386..>4510)
                     /locus_tag="VNY06_07395"
     CDS             complement(3386..>4510)
                     /locus_tag="VNY06_07395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX14659.1"
                     /translation="AETCARCHSSKLPDVAWKAMPDGCSGPDYLECFMRYWKLTQTDD
                     FKKKMLEIVLAPDFLEDNYLSTDARIPVTLLRTNACAPLATNAIRDNIWDNFSSSTYK
                     SLPSVGKITVQDPFTGEHWQYAMPGGGRGFTRVPSLVSEWANAPFLINKRIGKFDENP
                     SVEHRMKAFNEAIEQLLWPEKRKHDGNFDGEILRTTERSKVFIPKRQIPPELASILDF
                     PERYATLLKEPISLVKEPFSRLFDKDGNFEIGPFPKGFPIYLINYQPLVDIKDVGGKL
                     EHVNNGGALLLTFGANLPWPAKDASDEEMLGWLAKLRKPLLKLSKCPDFVVNKGHYFG
                     TAKFNDTEGLSDDEKSFVKDPEPVLKDDDKRALIEFLKTF"
BASE COUNT          997 a         1319 c         1203 g          991 t
ORIGIN      
        1 cagtaaacca atagccggag agcccccata gcgggtccga aaccggtagc cctaatttgg
       61 cgagggcgtc ggcaaggtcc cacttgagaa actttggctg atgcaaagac tcgacggttg
      121 gatggataaa aagtgtaatc caaacaaaaa aaagtaaaac cggtgcgacg atgagccagt
      181 ttgcgacaat gccgcggcca ataatgccaa gtgccgtgac aatatccaag gcaccgtgcg
      241 ggatgagata attggaataa tcccttatgt gccgaacaga gctggtgtcc gcatagtcct
      301 ctggtttgtc aacattcacg aatgggaact tgcattcggt cttttgcaag gcagcggtca
      361 gcgaagagcc aatatagccg ccccctgaca cggtcgaaag atagtcgatc ccttcaattc
      421 cataaacggg atgattttcg tgcttcgctt gcagagcctg aagaacgccg agcccgaacg
      481 acgccgacct tatgccgcca ccggaaagag cgaggcctgt gactggcgac cccgtcctct
      541 ctcgcccata cttatccttg ataaagtggc ggcgccgctc gatgacgtcg cgttcgacct
      601 tgcatacctg ctcgaacagc tgggtttggt cttcgggttt ccgcttgatc gtgtctggca
      661 tgccccttcc gcgcgggtcc actcctatag ccccggccca ggcccttcta attcagcgca
      721 ttaactcagc tttttgcatt tagaccggtg atgtagcgca aggccctgat gcctgggaga
      781 aagaaattag cgcaccgcgc atcgtgatga attgcggcac tccgcaaata cgttgggtaa
      841 gcccagaggg ctgcggcagg ctaaactcat tcgtcgattg accgaacggc gtcgcttcgc
      901 gatttccgac caacgggtca gattcgcggt caagcccatt aaactggctg ctgacaatcc
      961 aggcgttctg aatgaactcg aattgtcgcg aaatatagcc gacctggcgc aatgatcacc
     1021 gagccatcgg ccgagccatc cggaatggtc tcgactccga agcgctgaat aaatcgtcca
     1081 gctccttttg gctcatccga agcaactgag gaacatcata ggccatcgca tttccccctt
     1141 ttgatatgcc cacggcgatt acatcgccga ggccttgttc tcaaaatgtc agatgcgagg
     1201 attgcacaat tctttgttcg cttctagcac aatttaccgc gtagagcgcc gcagctcgaa
     1261 aacgcgagtt cggcaataat ccgcatcctg aggtcaattt gttatgcaac gcgagcgtcc
     1321 ttggtttccg ctaaaatgac gtcggcggcc ttttcggcga tcatatagat agcactcgca
     1381 atgaaaaaac cgggtatgcg gggaaacacg gaagcatcga caacgcggag cctcttcgtc
     1441 ccatgaacct tgaaatcgct cgttagcacg ccgcctgatc gcggcgggcc aatgggacac
     1501 gtgcaggacg cgtgatgacc ccaagcggcg tcgcgcacat agttggcgag ttcttccgag
     1561 gtctcaagct tctcgcctgg cagcttttcc gtcatcttta gcccgcgctc cttcatacgg
     1621 gcggtaatct tgcgcaccag gcgaatgccc tccaccaccg catcgagatc gttccccgcg
     1681 aggtcatcgc cctctccaaa ataacggaaa ttgatttcgg gcggctcgcg cggatcgttc
     1741 gagcgcagtg tgacaaagcc agcacgattg atggtatggg ccttcaatag ggcccaagtc
     1801 acatagttaa ggttgttggg attgcagcaa tccttgcggg aataatcggg ataataaccg
     1861 gtgaatttgc cgagcaacgc catgcagaac agatctggat caggcaggcc gggcgaagag
     1921 cgccgcgtga atgcaaccgc agcaccattc gaggtataaa tgccgcgccg gcgtttttct
     1981 tggtaacttt gtggcaaaat ttttcgccac cgcgccggca ataatctgaa tttcgggcgg
     2041 ttgagccact cctgaaacaa cggatcatcg ccgcgccggt attccgcgcc cttcaaggca
     2101 ttccaatcct tgtccagcca gttgacgacc ccgacctcat agcggtcttg gaggtttttc
     2161 ccgacgccct cgagcgggtg caccactctt atgccgcgtg cttcaagatc ttccttagga
     2221 ccgatccccg aaagcatcaa gagctgcggc gtgttgaagg cgccgccggc gagaataacc
     2281 tcccgcgctg gccggattgt gcagatctcg ccttcccccg cgctagcatc gtcatgcgcg
     2341 cgatagagcc gctctccctt tcggtattcc actccaacag cgcagttctc agcatcaaaa
     2401 agtacttttg tggcaagcgc gttacactca atctggagca ggcgtggatg gcgcgccgca
     2461 acttccaaca accgctcccg cgtgccgccg cgttgaccac cacgcgtcga aagtggcaca
     2521 aaacaggacc cttcggcccg cctccgcagg caccattggc cgtttggatt ggctccttcg
     2581 agcacaaaag gaatcaaggc cccaagggct agcttctcaa ccacgctccg caagccaagt
     2641 tgcactgctt tccacgcctg ttccagcgct tcccaaattt tggtgagccc ggcccgcttg
     2701 atcaaggtcg ccatccgctc gcgtcggata atccgcgaaa cgaacgatac cgtctcgcaa
     2761 ataagctcca tcagccggct gtcgtggacg gtttttcgcg gcaaggcgag ttcggtcgaa
     2821 agccacgggg gccatccctg ccccgtcaaa tcgtggtaga gccgggtaaa aaaacgccag
     2881 atcggccggt aacggcagtc ttcgacccgc cgcaaataac gccgcatgtt ttgcgcgctc
     2941 caggagccat cgcctgtgat gcgcgcgatc tcgtcccagt cggattcatg cggagccatg
     3001 aagatcatcg cattatgggc ggtgcagccg cccagcgttg cagcgcgggg ataataggtg
     3061 ctaggccttt gagtaagggg atctggcacg catttcggat ctgccctttg ccgctcgtca
     3121 tccgaataat gccgaacgta gaatttccag ctcatggccg gatgttcgga ggcgagcggg
     3181 tgaaaggccg gcaccgcata gtcctctggc attttttcgg cttgcttggc gggatcggca
     3241 ggatccccgc ccgcttccag caaaacgacg cgcatgccgg cttcggcaag ccttgccgca
     3301 agcgtgccgc cgccagcgcc agagccgaca acgaggtaat cgcattcaat catgcgccgc
     3361 gagcctcccg gtacacactc ccccgttaga aggtcttgag gaactcgatc aaggcacgct
     3421 tgtcatcatc tttgagcacc ggctccgggt ctttgacgaa tgatttttcg tcgtcgctaa
     3481 gtccctccgt atcgttgaac ttcgccgtgc cgaaataatg ccctttgttg accacgaaat
     3541 ccgggcactt gctgagtttc aacaaaggct tccgcaactt tgcgagccag ccgagcattt
     3601 cctcatcgct tgcgtctttc gctggccacg gtaaattggc gccaaatgtt aggagcaaag
     3661 ccccgccatt attcacatgt tccagcttcc ctccgacatc tttaatatca acaagcggct
     3721 gatagttgat gaggtagatt gggaagcctt tcggaaacgg ccctatttcg aaattgccat
     3781 ccttgtcgaa gagacgcgag aagggctcct ttacgagcga gatcggctcc ttcaaaagtg
     3841 ttgcatatcg ctctggaaaa tctaggatgc ttgcgagctc cggcggaatt tgacgcttcg
     3901 gaataaagac tttgctgcgt tcagttgtcc gcaagatttc gccgtcgaaa tttccgtcgt
     3961 gcttgcgttt ttcgggccac agcaattgct cgatcgcctc attgaacgct ttcatcctgt
     4021 gctcgaccga gggattctcg tcgaatttcc cgatccgctt gttgatgagg aagggagcat
     4081 ttgcccattc gctgacgaga gacggaacgc gggtaaaacc gcgcccgccc cctggcatcg
     4141 catactgcca gtgttcgcct gtaaatggat cttgaaccgt tatcttcccg accgaaggca
     4201 gggatttata ggtcgaggac gagaaattgt cccaaatatt gtcgcggatc gcattggtcg
     4261 cgagcggcgc gcaggcattg gtccgcagaa gcgtgaccgg aatgcgtgca tcggtcgaaa
     4321 ggtaattgtc ctccaggaaa tccggcgcca gcacgatctc aagcatcttc ttcttgaaat
     4381 catccgtctg cgtcaatttc caatagcgca taaagcattc aagataatca ggaccggaac
     4441 agccgtctgg catcgccttc caagcgacgt ccggcagctt gctcgaatgg cacctggcgc
     4501 aggtctcggc
//
LOCUS       DATMPU010000324         4920 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_4575517,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000324 DATMPU010000000
VERSION     DATMPU010000324.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 4920)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4920)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4920
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4575517"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(740..2854)
                     /locus_tag="VNY06_07400"
     CDS             complement(740..2854)
                     /locus_tag="VNY06_07400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020176946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HWX14660.1"
                     /translation="MPILAILVGIFSMIAVVTGFSSGDKTVLLLAVAGFLCTYTTYRS
                     RRVSTFLQIFAAIFAAETVIFGAIFFVAKVGLWPSSLADYEFPVSLPLTVAIFGILVY
                     AISFIPVVRSMTDIADRYFDASEPTHARIWPFPAFEIIERRLAIGMVVLLVVINQAQV
                     GMNLRLSFFSRDWFDSIQNKDEAAFWSQLFLVFVPWAFLFITTAVVEFVVTSTLVIRW
                     RRWLTGNYVSRWLGAHTHYRMSLAGGGADNPDQRISEDINRFIDGGQVDYGIYSFSIL
                     LIANLSSLVSFAILLWDLSANFTLPYTTIAVPGFLFWVALIYAGVGTLVTHLIGRPLV
                     RLSFERQRYEADFRFSLARLREYAEQVALLSGERTEKVSLMARFGAIVRNYFEIIACR
                     KNLTAFTSFYGQLSPIIPFVVAAPFYFAGKITLGIMTQTARAFSSVDSALTFFITYYV
                     SLADFKSVLDRLTSFEQAIDGARTPAFAPKESADMQAARKHLDIEALDVRLPDGREII
                     EGASVRLNAHEPVILTGPSGSGKSTLFRAISGIWPYGGGAITLPRDAKILLLPQRPYI
                     PIGLLRAAVTYPANPDSYDDGEVREALRNAQLSDLLGELDQADNWQQRLSGGEQQRLA
                     IARAILAKPDWLFLDEATSAMDEAMEAEIYDMLAAKLPRTTIVSIGHRASLAQFHKRH
                     LEMQPSGAGIFTPAEVNLKAAE"
     gene            complement(3039..3398)
                     /locus_tag="VNY06_07405"
     CDS             complement(3039..3398)
                     /locus_tag="VNY06_07405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004448201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HWX14661.1"
                     /translation="MRGGYEPRPERDDNRKTATRADLLNAVYTSCPGLSRAQAKQIFE
                     MALEEIADALVSGEPVKLRSFGLFTVRAKRERIGRNPRTGVEVPITPRRVLTFKPSPV
                     LTAAVNGESLEEEPEDG"
     gene            complement(3595..>4920)
                     /gene="groL"
                     /locus_tag="VNY06_07410"
     CDS             complement(3595..>4920)
                     /gene="groL"
                     /locus_tag="VNY06_07410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007602475.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HWX14662.1"
                     /translation="VAAGLNPMDLKRGIDLAVTAVVADLKKNSKKVTSNDEITQVGTI
                     SANGDKSIGEMISNAMQKVGNEGVITVEEAKSLETELDVVEGMQFDRGYLSPYFITNA
                     EKMIAELEDPYILVHEKKLSSLQALLPVLEAVVQTGKPLLIVAEDVEGEALATLVVNK
                     LRGGLKVAAVKAPGFGDRRKAMLEDISILTGGQMISEELGIKLENVTLAMLGRAKRVR
                     IEKEATTIIDGAGEKKDIEARIGQIKSQIADTTSDYDREKLQERLAKLAGGVAVIRVG
                     GASEVEVKEKKDLVDDALNATRAAVEEGVLPGGSVPLLRAMPALNGIKVENSDQQTGV
                     EIVRKAIQTPARQIIDNSGADGAVIVGKLIENPSYAYGYDAQTGEYGDLIKLGIIDPT
                     KVVRTALQDAASVAGLVITTEATVVEAPKKEAPAMPGGGGMGGMGGMDF"
BASE COUNT         1080 a         1411 c         1452 g          977 t
ORIGIN      
        1 agcttacgct gcccgcgttt caggtgggtc atgttcaacc ggggtcgacg cctcgtgcat
       61 gaggcatttt gattgccggg tcacagcaga tccggaccgt atgtaaggtg atgcagcgct
      121 cgctgttcag caaacgaccc cgatctccaa gccgccgcga tagggatctc aacggagatc
      181 caccaatcga gatccgtcaa tcaaactgcg ctatgaattg acaaaggaac cctcatgtaa
      241 ggatgaagcc cgcgtcagcc agcaaggcac ccgcatctgg gccaaacggg gcacggatca
      301 agcgcgaccg gcggtgggcc tacctcttcg gcgcaatctg cccggcgcgc ggcaccggcg
      361 ccgcgttggt catgccaaca gtcagcatcg aggccatgaa caaacccctg actgagatta
      421 tgagacgcgt cagcccgggc gccatcgctc gggtaatcct cgacgctgcc ggctggcacg
      481 gttcgccgca gtcgtcgtgc cacatcgtgc tcctgcctct gccgccttaa cgcctgaacc
      541 ccgcagcatc tgggagtatc ggcgcggcaa cgccctcagc aaccgcgtgt gggacaccta
      601 cgcggcaatc ctcgacgcct gttgcgacgc ctggaaccgt ctaatggcaa aatccaacgt
      661 catcacctca atcggaacca gagaatgggc acaggtcagg atttagggcg gttggacaag
      721 gcgcggggcg gaactcccat tattccgccg ccttgagatt cacttcagcg ggtgtgaaaa
      781 ttccggcacc ggaaggctgc atttcgagat ggcgcttgtg gaattgcgcg agactcgcgc
      841 ggtgaccgat cgatacgatc gtcgtgcggg gaagttttgc agcgagcatg tcgtagattt
      901 cagcttccat cgcttcatcc attgccgagg tcgcctcgtc aaggaagagc cagtcgggct
      961 tggctaagat cgcgcgggcg atcgcaagcc gctgctgctc gccccccgaa aggcgctgct
     1021 gccaattatc cgcttgatca agttcgccaa gaagatcgct aagctgcgcg ttgcgcagtg
     1081 cttcccggac ttcgccatcg tcgtagctat cggggtttgc gggataggta acggccgcac
     1141 gcaacaagcc aatcggaata tagggccgtt gcggcaggag gagaattttt gcgtctcgcg
     1201 gcagagtaat ggcgccaccg ccataaggcc aaatgccgga gatggcccgg aaaagggtcg
     1261 atttgccgga accggaaggg ccggtcaaga tcaccggctc atgggcgtta agccgcacgc
     1321 tcgcgccttc gataatctcg cggccatccg gcagccgcac atcgagcgcc tcgatgtcga
     1381 gatgtttgcg cgccgcttgc atgtccgcgc tctccttggg cgcaaaggcc ggagttcttg
     1441 ctccgtcgat tgcctgctcg aatgaggtca agcggtcgag gaccgatttg aaatcagcga
     1501 gcgacacata ataagtaata aagaaagtca aggccgagtc gacgctgctg aacgcccgtg
     1561 ccgtctgggt catgataccg agcgtaatct tgcccgcgaa atagaagggg gcagcgacga
     1621 caaagggaat gattggactg agctggccat agaaagatgt aaaggcagtg agattcttcc
     1681 ggcaggcgat gatctcgaaa tagttccgga cgatcgcgcc gaagcgcgcc atgagcgaga
     1741 ctttttcggt tcgctcgcca gagagcagcg cgacttgctc ggcatattcg cgcaatcttg
     1801 caagcgaaaa gcggaagtcg gcctcatagc gctgccgctc gaacgacagc ctcacgagcg
     1861 ggcggcctat caaatgggta acgagcgtgc cgacgccggc ataaatcaaa gcgacccaga
     1921 ataaaaaccc gggtaccgca attgttgtat aggggagggt gaaattggcg gaaagatccc
     1981 aaagcaggat cgcaaaggat acaagcgagc tcaagttcgc gatcagcaaa atcgaaaagg
     2041 aataaatgcc gtagtcgacc tggccgccgt cgatgaagcg attgatgtcc tccgagatgc
     2101 gctggtccgg gttgtcggct ccgccccccg caagcgacat gcggtagtgc gtgtgggcgc
     2161 cgagccagcg gctaacataa tttccggtga gccaccgccg ccagcgaatg acgagagtcg
     2221 aggtgacgac gaattcgacg acggcagttg tgatgaacaa aaatgcccag ggtacaaaaa
     2281 caagaaagag ctgcgaccaa aacgccgctt cgtccttgtt ctggatggag tcgaaccaat
     2341 cgcgcgagaa gaaggaaagt cgcagattca tgccgacctg cgcctggttg atgacgacaa
     2401 gcaagacgac catgccgatc gctaagcgcc gctcgataat ttcgaaggca gggaacggcc
     2461 agatgcgtgc gtgcgtgggt tcgctcgcat caaaatagcg atcggctata tccgtcatcg
     2521 accgcacaac cggaatgaag gagatcgcgt acacgaggat gccaaaaatt gcgacggtta
     2581 gaggtagact taccggaaac tcataatccg caagggagga gggccaaagg ccgactttgg
     2641 cgacaaaaaa tatggcacca aaaatcacgg tctcggcggc aaatattgcc gcgaagattt
     2701 gcagaaaggt agaaacacgc cgcgaccggt aggttgtata ggtgcagaga aatcccgcta
     2761 cggcaagcaa caagacggtt ttatcaccag aagaaaaacc ggtcacgacg gcgatcatcg
     2821 agaaaatacc cacgagtatg gcaagtatag gcattggtta ccttgatctt aagtttacgg
     2881 gggccaaggc ataaatggaa caaggagaac ggacaaaggc gcagcgcgca tcgccaggcc
     2941 actcggtcag gtctcggtaa aaaggaaggc gcgcgataga gccctttatc tgaagcgctt
     3001 ttatccaaat gttgcgtttt gaccaagcgc cagcttcgct aaccgtcctc cggctcttcc
     3061 tcaagagatt cgccgttgac cgcggcagtg agaacgggcg aaggcttaaa tgtaagcacg
     3121 cggcggggcg tgatcggcac ttccacgccg gtgcgcggat tgcggccaat gcgttcgcgt
     3181 ttcgcgcgca ccgtaaaaag accaaaggag cgtaacttga ccggttcacc gctgaccagc
     3241 gcgtcggcga tctcttcgag cgccatctcg aagatttgct tcgcctgagc gcgcgatagg
     3301 ccggggcagg acgtgtagac ggcgtttagg agatccgctc ttgtcgcggt tttccggttg
     3361 tcatcccttt ccggccgggg ctcgtaccct ccacgcacct tgctggctct ccccgttgcg
     3421 tctgtctcgt tggagctgat cttagatcat tttgcgggcg agaggaaact ggcgcgcttg
     3481 agggctccgc aatttaattt gaggaagagc gagaaacgct gcggccggcc ggctcgtgcc
     3541 aacaaaaagg ccccggagtt ccggggcctt ctcgcaagaa ttgatggggc ggctttaaaa
     3601 atccattccg cccattccgc ccattccgcc gccgccgggc atcgcgggcg cctctttttt
     3661 cggcgcttcg acgactgtcg cctcggtggt tatgacaaga ccggcaaccg aagccgcatc
     3721 ttgcaacgcg gtgcgtacga ccttggtcgg gtcgatgata ccgagcttta tcaaatcgcc
     3781 atactcgcca gtttgggcat catatccgta ggcgtaggac ggattttcga tcagcttgcc
     3841 aacaatgacc gcgccatcgg caccggaatt gtcgatgatc tgccgcgccg gtgtctgaat
     3901 cgccttgcgg acaatctcga ccccagtctg ctggtcggag ttctcgacct taattccgtt
     3961 caaggctggc atggcgcgca agagcggcac gctgccgcca ggcagcacgc cttcctcgac
     4021 ggccgcgcgg gttgcattga gcgcgtcgtc gacaaggtcc ttcttttcct tcacctcgac
     4081 ttccgaggcg ccgccgaccc ggatcaccgc gaccccgccg gcgagcttcg caagacgctc
     4141 ttgaagcttc tcgcggtcat agtccgaagt ggtatcggcg atctgcgact tgatctggcc
     4201 gatccgcgcc tcgatatctt tcttttcgcc ggcgccgtcg atgatcgtcg tcgcttcctt
     4261 ttcgatgcga acccgcttgg cgcggccgag catcgccaaa gtcacgtttt cgagcttgat
     4321 tccgagctct tccgaaatca tctgacctcc ggtcagaatg gaaatgtcct cgagcatggc
     4381 cttgcggcgg tcgccgaagc ccggggcctt gacggccgcg acttttagtc cgccgcgcag
     4441 cttgttgacg acaagcgttg ccagcgcctc gccctcgacg tcctcggcga cgatgagaag
     4501 cggcttgccc gtctgtacga ctgcttcgag aaccggcagc agggcttgca gcgaagacaa
     4561 tttcttttcg tgcacgagaa tataagggtc ttcgagctcg gcgatcattt tctcggcatt
     4621 ggtgataaaa tagggcgaaa gatagccgcg gtcgaactgc atgccttcga cgacgtcgag
     4681 ttcggtttca aggctctttg cctcctcgac cgtgatgacg ccttcattgc cgaccttctg
     4741 catcgcgttc gaaatcattt cgccaatcga cttatcgcca ttggctgaga tcgttccgac
     4801 ctgggtgatc tcgtcattgg aggtaacttt tttggagttc ttcttgagat ccgcgaccac
     4861 ggcggtgacc gcaagatcga tgccgcgctt caaatccatc ggattgagac cggcggcgac
//
LOCUS       DATMPU010000325         4000 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_882890,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000325 DATMPU010000000
VERSION     DATMPU010000325.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 4000)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4000)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4000
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_882890"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..194
                     /locus_tag="VNY06_07415"
     CDS             <1..194
                     /locus_tag="VNY06_07415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX14663.1"
                     /translation="GLGPNAIERRSGSRDGSSPRRQTDLASSGACQALARALPRLLIR
                     RRIWQADGLPLLQCRAEPR"
     gene            complement(436..894)
                     /locus_tag="VNY06_07420"
     CDS             complement(436..894)
                     /locus_tag="VNY06_07420"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: protein motif:HMM:NF014641.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HWX14664.1"
                     /translation="MAICEAIRVEKVATAAEIGAVLRAGRRAPDGGVIWWPRSTADNP
                     GTQEPGARSLRTASGCRRRHRLALPLVQILDLEDTSVCEVWIDQLVAGSFQDVIWLTG
                     EGLRLLLSFAEQSGHRPPFVEALRGVRTITRGPKPARVLRELGLAPISQR"
     gene            1795..1959
                     /locus_tag="VNY06_07425"
     CDS             1795..1959
                     /locus_tag="VNY06_07425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX14665.1"
                     /translation="MLYRIGIFLLIAVALGLLWPRKIRAALGFVGLIAVLVAVGFLIV
                     VLINLPTPQQ"
     gene            complement(2067..2921)
                     /locus_tag="VNY06_07430"
     CDS             complement(2067..2921)
                     /locus_tag="VNY06_07430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010661770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate resistance protein ChrB
                     domain-containing protein"
                     /protein_id="HWX14666.1"
                     /translation="MLIHQIPAKPAYLRVKIWRRLQTLGAVMVKNAVYALPASEQTQE
                     DFEWVSKEICEGGGEALICEARLIDGLSDQEVRTLFNTAREEDYDLLAKDARVLADTL
                     RQAPTADAKAEAKAQFAKLKTRADQVLAIDFFGANGREALDGLLAGLEEALREDEVAQ
                     PIRSEPGHGSLRDLSGRVWVTRRGVHVDRIASAWLIKRFIDQAARFKFVPSKGYVPKT
                     DELRFDMFDAEFTHVGDRCTFEVLLASAALEDSALEAIAEIVHDIDLKDEKFGRAEVD
                     GINYDLQL"
     gene            3082..3540
                     /locus_tag="VNY06_07435"
     CDS             3082..3540
                     /locus_tag="VNY06_07435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017677027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate resistance protein ChrB
                     domain-containing protein"
                     /protein_id="HWX14667.1"
                     /translation="MKWITRERPKIDRIACPWLIKRFIDKDPEFLFVPAPRVLAVAAE
                     TGAVPYDVPDVELTHVGELCSFDAFLQRYQLIDPALTQLAVIVRGADTGRPELAPQAA
                     GLLAVSLGLSAIFADDHELLGRGMVIYDALYAWCRSCQGEVHSRPPNMAA"
     gene            complement(3585..>4000)
                     /locus_tag="VNY06_07440"
     CDS             complement(3585..>4000)
                     /locus_tag="VNY06_07440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003615118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="porin"
                     /protein_id="HWX14668.1"
                     /translation="NFSGYYVYATWYLTGESRAQAYRTYPGTYNFPGTFDQIHIINPV
                     SAGGTGAWEAAVRLSEINLNSGGFLFAQPVGLPSNIQGGRETDLTLGLNWYPDTGIRF
                     MANWVDVLQLAAPWNRPNINGIHPQIFELRAQVNG"
BASE COUNT          856 a         1152 c         1151 g          841 t
ORIGIN      
        1 caggacttgg cccaaacgca atcgagcgcc ggtcaggatc aagggacgga tcgtcgccgc
       61 ggcggcaaac ggatctagcc tcgtccggcg cctgtcaggc tttggcgcgt gctttgcccc
      121 ggcttttgat tcgccgacgc atatggcagg ccgatggtct gcccctcctt caatgccgcg
      181 ccgagccgcg ctagttcggc gctcgtgaga taccgttcgt tgttcaggat agcgctcaat
      241 tgccatagca gggttatctg caaacgccac ttcgccgcgc tgggccgccc agttccacac
      301 ggtgatatga gcgccagcgc gcggttgcct catatgcttg ccataaagct ttgccacatc
      361 ggcttgccta agccgatcga ccggccggag atgtcttctt tagcgagcgc atcgcaaacc
      421 cctgcgatgt tggtgtcagc gctgcgagat gggggcgagg ccgagttccc gcaacacgcg
      481 agcgggtttt ggaccgcgag tgattgtgcg cacgccgcga agcgcctcaa cgaatggcgg
      541 gcggtgtccg ctttgctccg caaaagagag gaggaggcgc agaccctcgc ctgtgagcca
      601 aatcacgtcc tgaaacgacc cggcgacaag ctggtcgatc caaacttcgc aaacagaagt
      661 atcttcgagg tcgaggattt gcacgagcgg caacgcaagg cggtggcgcc ttctgcatcc
      721 agaagccgtg cgaagacttc gggctcccgg ctcttgggta ccaggattgt cagccgtcga
      781 tcgaggccac catatcacgc cgccgtcggg agctcggcgc ccggcgcgta gcacggcacc
      841 gatttctgcg gcggtggcga ccttttcaac ccggatcgct tcgcagatgg ccacctcacc
      901 gatcgaaaaa gcaggagcag tcggttggcg gtgcagctgc ttcgacgcct gccagaagcg
      961 aaaaccggtg actcggttcg agcctctggc caagcaattg cgaagcccgg aaggcctgcg
     1021 ggaaagcggc gcgagacccc gcaaaaaaca cgcaagcttc taagcggcag tctttgaggc
     1081 cggcatagcg gattccggtc ttttcgggct ggaataagag ataagctcag cctcttttgg
     1141 catcgcaaga aggctccgta gggcgtgttt ccgcaaatcg gattatgaga gcttgtcgtc
     1201 aagcgctttc gcgcgttcgg catccgtatc aataagcaag attttgaggg gcatcggcag
     1261 aatcgtttca cgaaacttga agcgccacaa tataacaatc tacgcgcctg gcggcttaag
     1321 ctgctttcca cgcccagaaa agacacgccc agaagaacca tgccagctaa tctcccttgc
     1381 gtccgcgagc tggatatatt tcacgacgac acgccacgcg tgatccgtga aggctatcgc
     1441 caaagggctc aatgcgcctg aatgtctgcc atcaatattc ggggtcggca aaaaatcttg
     1501 taccagtacc cccgaatagg cagatcaacg actagcgacc aaatcattat ataccgcaga
     1561 tcgccgtact taatgcttgc tgcccgccgg tcttagaaaa ttgtatgagg cgtcttctcg
     1621 gcatcgcacg ttgtagttgc tatctccata agtccggcac ttgccggcat gggaagaggc
     1681 aagtacaccc aagcaaggaa aagaattggc gaattgcaaa gtcccggaca gccaacgccg
     1741 cgaattggtg caaatgggtt gcagatgaat acgtaaggcg aagtgagcgg ctcaatgctc
     1801 taccgaattg gaatttttct gctcattgca gtcgcactcg ggctcctttg gccgcgcaag
     1861 atacgggctg ctctaggttt tgtgggcctt atagctgtgc ttgtggccgt tggattttta
     1921 atagtcgtgc tgattaactt gcccacccca caacagtaat cacgccgctg ccggcccctg
     1981 gagatagacc agcagcggga cacttgggtg ctgaaacggg tgctcccacg atgagtagga
     2041 gcaaaagatg aaacaaaata tttgcgctat agttgcagat cataattaat tccgtcgacc
     2101 tccgcgcggc cgaacttttc gtctttgaga tcgatgtcat ggacgatctc ggcaatcgct
     2161 tcgagcgccg agtcctcgag ggcggcgctt gccagcaaga cctcaaaagt gcagcgatcg
     2221 ccaacatggg tgaattctgc atcgaacata tcaaagcgga gttcatcggt ttttggcacg
     2281 tatcccttgc tgggaacgaa cttgaaccgg gctgcctggt cgataaatct tttaatcagc
     2341 caggcggaag cgatacgatc gacgtgtaca cctcggcgtg tgacccagac ccgcccgctt
     2401 agatcacgga gtgagccatg acccggctcg ctccggatcg gctgggccac ctcatcttcc
     2461 ctgagagctt cctccaaccc ggcaagcagt ccgtcgagag cctcgcggcc attggcgccg
     2521 aagaaatcga ttgccaggac ttggtccgct cgggtcttca gtttggcgaa ttgggccttg
     2581 gcttccgctt ttgcatcagc ggttggagcc tgccgcagcg tatcggccaa cacccgggcg
     2641 tccttggcga gcagatcata gtcttcttcc cgtgccgtat tgaacaaggt tcgaacctcc
     2701 tgatcagaca ggccatcgat caggcgagct tcgcagatca aggcctcgcc gccgccctca
     2761 cagatctctt tcgacaccca ttcgaagtcc tcttgggtct gctcactcgc cggcaacgcg
     2821 tagactgcgt tcttgaccat cacggcgccg agcgtttgca accggcgcca gatcttcacc
     2881 cgcaaatagg cgggcttggc ggggatctga tgaatcagca acagccagcg atcctgcgag
     2941 tctgtgccgg aatcggtctc tactctagca ttcacgtaac aaccatatca caaaaaaggt
     3001 gagataacga aacacttgta tcatgacagt ttttgcgtat cctttaggcc tcgtggggct
     3061 caaacgcaac gaggatacgg catgaaatgg atcacccgcg agcgtcccaa gatcgaccgg
     3121 atcgcctgtc cctggcttat taagcgcttc atcgacaaag atccggagtt tctctttgtt
     3181 cccgctccgc gcgtgttggc agtggcggct gagacaggcg cggtacccta tgacgtgccg
     3241 gacgtcgaac tgacccatgt gggggaactc tgcagtttcg atgccttttt gcagcgctat
     3301 cagctgatcg atccagccct gacccaattg gcggtgatcg tgcgcggtgc ggataccggc
     3361 cggccggagc tcgcgccgca agccgcgggc ttgcttgccg tctcgctcgg cctttcggcc
     3421 atattcgcgg atgatcatga gctgctgggg cgaggaatgg tcatttatga cgcgctctac
     3481 gcatggtgcc gatcctgcca gggagaggtc cattccaggc cgccgaatat ggcggcctga
     3541 taggcgacaa tctttgagcc actctgacag cggagaaaac catctcaccc gttgacctgg
     3601 gcgcgcagtt cgaaaatttg cgggtggatc ccgttgatat taggccggtt ccagggcgcc
     3661 gcgagctgaa ggacatcgac ccagtttgcc atgaagcgga tgccagtatc gggataccaa
     3721 ttgaggccca gggtcaaatc cgtctctctg ccgccctgga tgttggaagg tagaccaacg
     3781 ggctgggcga aaaggaagcc accagaattc agattgattt cgctgagccg tacggcggct
     3841 tcccacgcgc cggtaccgcc cgcgcttacg gggttaatga tatggatctg gtcgaaggtg
     3901 cccgggaaat tgtatgtccc aggatatgtc cgataggctt gcgcgcgcga ctccccagtc
     3961 agataccaag tggcatagac gtagtatccg ctgaagttga
//
LOCUS       DATMPU010000326         5165 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_2404009,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000326 DATMPU010000000
VERSION     DATMPU010000326.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 5165)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5165)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5165
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2404009"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            10..342
                     /locus_tag="VNY06_07445"
     CDS             10..342
                     /locus_tag="VNY06_07445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020176949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX14669.1"
                     /translation="MSKIFVLTMFVTSSVMLAGCGYTPEQRGLSGAALGGATGAAIGA
                     STGGGAGAAVAGGALGAATGALVGAATAPPPPPPPPVYGAPPPPPRCARFGYDAYGNR
                     ICTAYYGY"
     gene            339..1091
                     /locus_tag="VNY06_07450"
     CDS             339..1091
                     /locus_tag="VNY06_07450"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007770792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HWX14670.1"
                     /translation="MKSDASLPATLIPSPNHGVRKGGRLPDSILLHYTGLPDGETALR
                     QLCDPATEVSSHYLVWEDGRLAQLVPESRRAWHAGRGVWAGESDMNDVSIGIEIANGG
                     HRKGLPDYPSVQIETVIALCHDIIERWRIRPERVLAHSDVSHGRKIDPGEHFPWWRLA
                     KAGIGHYVPPSAIEDGPHLEPGATGPKVLELQAMLVRYGYGVDMTSLYDARTESAVTA
                     FQRHYRQFLVDGLADYSTIETLRRLIDALTKA"
     gene            complement(1151..4270)
                     /locus_tag="VNY06_07455"
     CDS             complement(1151..4270)
                     /locus_tag="VNY06_07455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007565343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HWX14671.1"
                     /translation="MNPSRIFILRPVATTLLMAAIMLAGGVAYRFLPLSALPEVDYPT
                     IAVQTFLPGASPEVMTSSVTAPLERQLGQMPGLNQMTSASSAGASVITLQFSLDLSLD
                     VAEQEVQAAINTANNLLPSGLPTPPVYAKVNPGDAPVMTLAVTSKTLPLTEVEDISET
                     RLAQKISQQPGVGLVSINGGHRPAVRIKFNPTALAAYGLNIDDLRTTIANGTVNAPKG
                     SFDGPAQASTINANDQIDSAPGFRSLVIAYRDGRPVRLSDVASADSGPENSKLAALTN
                     TTPAIILSIRRQPGSNVIEVVDSIKRLLPGIVASLPPAIDVKVLSDRTGTIRASVADV
                     ELELTMAVALVIATIFLFLRTLPATIIPSLSVPLSLVGTLAAMYLFGFGLDNLSLMAL
                     TISTGFVVDDAIVMIENITRYVEAGDPPLQAALKGSEQIGFTIISLTVSLLAVLIPLL
                     FMGDVVGRLFHEFAVTLAVTIVISAIVSLTLVPMLCARLIRRHSLTDRSRFDLAAERA
                     FNGLLVLYNRALVVVLQHQPLTLLIAVATLALTIVLYILIPKGFFPVQDTGMIQAIAE
                     APQSISFPAMAGLERQIAEAILRDPAVESVSSFIGVDGDNATLNSGRFLINLKPRTER
                     AASATEVARRMQNAVTGMPGVALHLQPVQDLTIDSSVSRAQYHLILQNPNPGDLKLWA
                     QRLTQRLAQLPELGDVASDSQGQGLYVELFIDRAAAARYGITPATVDNALYDAFGQRI
                     ISTIYSQSNQYRVIMEADPSLQQALSSLSSFYLPSSTSGQNGQVPLSSIVQVSQYQGP
                     LLITHLGQFPAVTISFNVAHGASLGAAVEAIEKAGREIGLPASFLISFQGAAAAFRAS
                     ATNELFLIIAAVIAMYIVLGVLYESFVHPVTILSTLPSAGVGALLALIVSGHELDVIA
                     IIGIILLIGIVKKNAIMMIDFALDAERLDGIAPQEAIFQACLLRFRPILMTTFAAILG
                     AMPLMLGTGTGSELRNPLGIAIIGGLVVSQILTLFTTPVIYLYFDRLATRLGGRGAGS
                     PLAAGSLRE"
     gene            complement(4308..>5165)
                     /locus_tag="VNY06_07460"
     CDS             complement(4308..>5165)
                     /locus_tag="VNY06_07460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012589221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HWX14672.1"
                     /translation="DQARKNLVRFQTLLKQDSIARQQAEDQVFLVKQSEGSVQADQAQ
                     IDAQKLNLIYAHIVSPIDGRIGLRMVDPGNFVQTTDAGLAVVTQMHPISVIFIVPEDD
                     IPPIMDAIRAGRTLEVTAYDRANVAPLSTGKVVSLDSQIDPTTGTVKLRADFDNLDDK
                     LFPNQFVNARLLTKTLHGAITVPSRAVQYGAPGAYVYLVGADDTVSARAVELGPQDGP
                     MFAVKSGLSPGDRIVVEGADRLRPGIKVSINAPKEPDGGATSRLTDSPESGKNQPKNE
                     PGLRSGDKP"
BASE COUNT         1124 a         1407 c         1570 g         1064 t
ORIGIN      
        1 ggaagtggca tgagtaagat atttgtactg acgatgtttg tcacatcgtc tgtcatgctc
       61 gccgggtgcg gctatacgcc ggaacagcgg ggactgagtg gtgcagcgct tggcggcgcg
      121 accggtgcgg caattggagc ctccacgggc ggtggggctg gcgcggcggt tgctggcggg
      181 gcgcttggcg ccgcgacggg cgcccttgtt ggagctgcaa ccgccccgcc tccgccccca
      241 cctccgcctg tttatggtgc gcccccgccg ccgcctcgct gcgcacggtt tggctatgac
      301 gcctatggga atcgaatttg cacggcctat tacggctatt gaaatctgac gcttctttgc
      361 ctgcgacgct cattccttcg cccaatcacg gcgtacgcaa aggtggccga ttaccagatt
      421 cgattttgct gcattacacc ggtctgccgg atggcgagac tgctttgcgg cagctgtgtg
      481 atccagcaac agaagtgtcg tctcattatc tcgtctggga ggatggccgc ctcgcgcaac
      541 tcgtgccgga atcccgacga gcttggcacg cgggccgggg tgtttgggcc ggcgagagcg
      601 atatgaatga tgtttcgata gggatcgaaa tcgctaatgg gggacatcgc aagggattgc
      661 cagattatcc gtcggtgcag atcgaaaccg tcatagcgct ttgccatgat attattgaac
      721 gctggcgcat taggccggag cgggtgctcg cgcattctga tgtatcccat ggtcgtaaga
      781 tcgaccctgg cgaacatttt ccctggtgga gattggctaa ggccgggata ggccattatg
      841 tgcccccgtc cgcgatcgag gacgggccgc accttgaacc cggtgcaacc ggtcccaagg
      901 tcttagagct tcaggccatg ctcgttcgat atggctatgg tgtcgacatg accagtcttt
      961 atgacgcgag gacggagtct gcagtcacgg ctttccagcg ccactatcgt caatttctgg
     1021 tcgacgggct cgcagattac tcaacgattg agaccttgcg caggctaatc gacgcattga
     1081 ccaaagcata ggccggcacc aatcgagcca gcatgtgcca ttgcaacgcg cggaaaagca
     1141 gcgaggggaa tcactctcgc aggcttcccg cagcaagcgg gcttccggca ccccttccac
     1201 ccagccgcgt cgccaggcga tcgaaataga gatagatcac tggcgtcgta aagagcgtca
     1261 agatctggct cacgacgagc ccgccgatga tggctatgcc gagcgggttg cggagttccg
     1321 agcccgtgcc agtcccgagc atcaacggca tcgcaccgag aatcgcggcg aaagtcgtca
     1381 tcaggatagg gcgaaatcgc aacagacacg cctgaaagat agcctcctgt ggtgcgattc
     1441 catccaagcg ttcagcatcg agcgcgaagt ctatcatcat aatcgcattc ttcttgacga
     1501 ttccgataag taatatgatg ccaatgatcg caatgacatc gagctcatgg ccggacacta
     1561 tcaaagcgag cagtgcacct acacccgccg acggaagcgt cgagaggatc gttaccggat
     1621 ggacgaagct ttcgtagagc acgcccagca cgatgtacat cgcaattacc gcggcgatga
     1681 ttagaaaaag ctcattggtt gccgaggccc ggaaggctgc ggccgcgcct tgaaaggaga
     1741 taaggaaact agctggcagg ccgatttcgc gcccagcctt ttcgattgct tcgacggccg
     1801 cgccaagtga ggcgccgtgt gcgacattga aggaaatcgt aaccgccgga aattgtccaa
     1861 ggtgggtgat caacaaaggg ccttggtact gactgacctg gacaattgaa gaaagcggaa
     1921 cctgcccatt ttgacccgat gtcgacgagg ggaggtaaaa cgacgaaagg ctggagagcg
     1981 cctgctgcaa cgaagggtca gcttccatga tgacccggta ttgattcgat tgcgaataga
     2041 tggtcgaaat aatgcgttgc ccaaacgcgt catagagcgc attgtcgacg gtcgccgggg
     2101 taattccata gcgggcggca gccgccctat cgataaacaa ttcgacataa agcccttggc
     2161 cctggctatc actggcaaca tcgccaagct ccggtagctg ggcgagcctt tgcgtgagcc
     2221 tttgcgccca aagtttaaga tccccaggat tggggttttg caggatcagg tgatattgcg
     2281 cgcggctgac actggaatcg atcgtcaggt cctgcacggg ttgcaaatgc agcgcgaccc
     2341 cgggcatacc agtcacagca ttttgcatcc ggcgggcgac ttcggttgca ctcgctgccc
     2401 gctctgtccg gggcttgaga ttgatcaaga accggccgct attcagtgtc gcgttgtcgc
     2461 catcgacgcc gataaaggag ctcacgcttt cgactgccgg atcgcgcaag atcgcttccg
     2521 ctatctggcg ctcaaggcct gccatggctg ggaaggaaat gctttgcggc gcttccgcaa
     2581 tcgcctgaat catgccggtg tcctgaaccg ggaaaaaccc tttgggaatt aggatgtaga
     2641 gcacaatcgt gagggcaagc gtggcgacgg caatcaatag ggtgagcggt tggtgctgca
     2701 gcacgacaac aagtgctctg ttgtaaagga caagcaatcc gttgaaagcc ctttccgccg
     2761 caaggtcaaa gcgggagcga tccgtcaaag aatggcgccg gatcaaccgg gcgcagagca
     2821 taggcacgag agtgagcgag acaatcgctg agatcacaat cgtgacggct agtgtgaccg
     2881 caaattcgtg gaacaggcgg ccaaccacat cgcccatgaa taggagcggg atcaggaccg
     2941 cgagcaggga aacagtcaac gaaattatgg tgaaaccgat ctgttccgaa cctttgagcg
     3001 ctgcttgcaa aggtggatct ccagcttcga catagcgcgt aatattctcg atcatgacaa
     3061 tcgcgtcatc gacaacgaag ccggtcgaaa ttgtcagggc catgagcgaa agattgtcga
     3121 ggccgaagcc gaacaaatac atcgcggcaa gcgtgccgac gagcgacaag ggcacggaaa
     3181 ggctcggaat gatcgttgcc gggagagtac gcaggaacaa aaaaatcgtc gcaatgacca
     3241 gcgccacggc catagtcagt tcgagctcaa cgtcggcgac cgaagcgcga atggttcccg
     3301 tgcggtcgct tagcacctta acgtcaatgg ccggcgggag gcttgctacg atcccaggca
     3361 agaggcgttt gatcgagtcc acgacctcga tcacattcga tccaggctgg cggcgaatgc
     3421 tcaggataat ggccggagtg gtattggtca gggccgcgag tttggaattt tctggcccgg
     3481 agtcagcgct tgcaacgtcc gagaggcgaa ccggcctgcc gtcacgataa gcaataacaa
     3541 gggaacgaaa gccgggggcg ctatcgatct ggtcgttggc gttgattgtc gaggcttgtg
     3601 ccggaccgtc gaaactcccc ttgggcgcat tgaccgtgcc gttggcgatt gtggtgcgaa
     3661 ggtcatcgat gttgaggcca taagcggcga gtgccgttgg attgaatttg attcgtactg
     3721 ctggccggtg acccccattg atgctcacca gaccgacgcc gggctgctgt gagatttttt
     3781 gtgcgagccg tgtctcgctg atgtcttcga cttcggtcaa tggcaaggtt ttggatgtta
     3841 cagcgagcgt catcaccggc gcatcacccg ggttcacttt ggcgtaaact ggtggagtcg
     3901 gaaggccgct tggcagcaga ttattagccg tatttattgc tgcttgaacc tcctgctcgg
     3961 caacatcgag actaaggtct aggctgaact gcaatgtaat cacggacgcg ccagccgagc
     4021 ttgcggacgt catttgatta aggcccggca tctggcctag ctggcgctca agtggcgccg
     4081 tgaccgaaga tgtcatgacc tccggacttg cgccaggaag aaaggtttgc actgcaattg
     4141 tcggatagtc tacctcaggc aaggcagaaa gcggcaggaa gcggtaagcg actccccctg
     4201 cgagcatgat tgcggccatg agaagcgttg tcgcgaccgg cctcaggatg aaaatgcgag
     4261 acggattcat tgcccgcgcc cagcattgga tgccggttcg gcctcagtca tggcttatcc
     4321 ccggaacgca atcctggctc gttttttggc tggttcttcc cagattccgg gctgtcggtc
     4381 agccgggaag tcgcgccgcc gtctggttcc ttgggagcgt tgatcgacac ctttattccc
     4441 gggcgcaagc ggtctgcgcc ttcgacgacg atccggtcgc ccggcgaaag gcctgatttt
     4501 acagcgaaca tggggccgtc ctgtggcccg agctcgacgg cacgcgctga caccgtatcg
     4561 tctgcaccaa caaggtagac gtaagctcct ggcgcgccat actgtaccgc gcgcgagggc
     4621 acggttatgg cgccgtgcaa agtcttggtc aaaaggcgcg cattaacaaa ctggttcggg
     4681 aacagcttgt cgtcgagatt gtcgaaatcc gcgcgcaatt tcacggtgcc agtcgtcggg
     4741 tcgatctggc tgtcgagcga tacaaccttg ccagtagaaa gtggcgcgac attcgcccgg
     4801 tcataggcgg tcacctctag cgtcctgccg gcgcgtattg catccatgat ggggggaata
     4861 tcatcctccg gcactatgaa aatgaccgag atcggatgca tctgggtcac gactgcaagg
     4921 ccggcgtcag tggtctgcac aaaattgccg ggatcgacca tgcgaaggcc aatgcggcca
     4981 tcgattggcg aaacaatatg cgcgtagatc aagttcagtt tctgcgcgtc aatctgggct
     5041 tgatctgctt ggactgaccc ctcggattgc ttgacgagaa agacttggtc ctccgcttgc
     5101 tggcgggcaa tcgagtcttg cttaaggagc gtctgaaatc gcaccaaatt cttgcgcgcc
     5161 tgatc
//
LOCUS       DATMPU010000327         2338 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_2404867,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000327 DATMPU010000000
VERSION     DATMPU010000327.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 2338)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2338)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2338
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2404867"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            195..1088
                     /gene="dapA"
                     /locus_tag="VNY06_07465"
     CDS             195..1088
                     /gene="dapA"
                     /locus_tag="VNY06_07465"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012589888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HWX14673.1"
                     /translation="MAKKAAFRGSITALVTPFHDGRFDEHRFRALVDWQVVSGTHGLV
                     PVGTTGESPTLSHEEHRQVIAACVREATGRIPVIAGAGSNNTQEAIELARFAEGAGAD
                     GLLIVTPYYNKPSQEGLFRHFKAVNDAVGIPIIIYNIPPRSVVDMSIDTMKRLYELKN
                     IAGVKDATGNLARTALQRHELGPDFIQLSGEDMTALAVMAHGGHGCISVTSNVAPALC
                     AQMQEACVGGNFTKALKLQDQLTPLHAALFVEPNPAGPKFALSLLGKILDELRLPMLP
                     ASDSAQAAVKAAMIHAGLISA"
     gene            1317..1793
                     /gene="smpB"
                     /locus_tag="VNY06_07470"
     CDS             1317..1793
                     /gene="smpB"
                     /locus_tag="VNY06_07470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011473077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HWX14674.1"
                     /translation="MAAKQERPRKIAAENRRARYNYQIGETFEAGLMLTGTEVKSLRT
                     GKANIAESYASVDRAGEIFLINANIPEYLQANRFNHEPKRPRKLLLKSREIAKLAQGT
                     ERQGMTIVPLKIYFNEKGRAKIEIALARGKQLHDKRETEKARDWNREKGRLLREKG"
     gene            2063..>2338
                     /locus_tag="VNY06_07475"
     CDS             2063..>2338
                     /locus_tag="VNY06_07475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX14675.1"
                     /translation="MDIPAHLPHSIAPANVLLQAVLPANVDQIPAMPLPQKQRGEYLS
                     LRREFEPTSVRLVIVAESPPISGNYFYNPDGEVSEQLFKALMEQLGIK"
BASE COUNT          544 a          649 c          652 g          493 t
ORIGIN      
        1 gtccctcgca taagccgctg ggcggattga atcggcggtc tgatctcgac caaaagagcc
       61 ctttccggaa acgaaatcag agtctatgtc tccgttcgtg tccctgccgg gttctcaatt
      121 ggactgctgc gtctatcggt agaagagaat cccataaatt gcggcgtcac ttgggcggaa
      181 aggcggtatt aaccatggca aaaaaggcag ctttcagggg atcgataacc gcacttgtca
      241 cgccattcca tgatgggcgt ttcgacgaac atcgtttccg cgctctggtc gattggcagg
      301 tcgtgagcgg cacgcatggg ctcgtaccag ttgggacaac aggcgaaagt ccgaccctgt
      361 cgcacgaaga gcaccggcaa gttatcgccg cctgtgtccg cgaagccaca ggacgaatcc
      421 cggtcatcgc cggggcaggg tcgaacaaca cgcaagaagc catcgagctt gcgcgtttcg
      481 cggagggagc tggcgccgac ggtctcttga tcgtcacgcc ctactacaac aagccttcgc
      541 aggagggtct ctttcgccat ttcaaagcgg tcaatgatgc cgttggtatt ccaattatta
      601 tctacaatat tccgccgcgc tcggtcgtcg atatgtcgat cgacaccatg aaacggctct
      661 atgaactcaa aaacatcgcg ggcgtcaagg atgcaacggg caatcttgcg cgcacggcct
      721 tgcagcggca tgaacttggg ccggatttta tccagctttc cggcgaggac atgacggctc
      781 ttgcggtgat ggcgcatggc ggccacggct gtatttcggt gacttcaaat gtcgcgcctg
      841 cgctttgcgc gcaaatgcag gaggcgtgcg tcggcggcaa cttcaccaaa gctctcaagc
      901 ttcaggatca attgacaccg ctccatgccg ccctcttcgt tgagccgaat cccgcaggtc
      961 caaaattcgc gctttccttg ctcggcaaga ttcttgacga actacgcctt cccatgctgc
     1021 ccgcttcaga ttcggcgcag gccgcggtca aggcggcgat gattcacgcg gggctcattt
     1081 cagcgtgaaa tgtcggattt gatccatccg cccgtgtcag atctcgcaaa tcaactcacg
     1141 gcgagcatta tcggcgacgc gggctcgatt ctgtcaagga tgtgaagcac cggcttcgcc
     1201 ggcgcgagcg cagctcgcgt ccttgacaga atcgatccgc tcgccaaact cgccgccaag
     1261 agatgaatgc gtgttgcgat ttggggcgac cgcggctttc acttgcggag tgttaggtgg
     1321 ctgccaagca ggagcgcccg cgcaaaattg cggcggaaaa ccggagggcg cggtacaatt
     1381 atcagattgg cgaaactttt gaggctggcc tgatgctcac gggcaccgag gtaaaatcct
     1441 tgcgcaccgg caaagccaat atcgcggaga gctatgccag cgtcgatcga gccggagaaa
     1501 tctttctcat caatgccaat atcccggaat atttgcaggc caaccgcttc aatcatgagc
     1561 ccaagcgtcc gcgaaaactg cttttgaaat cgcgcgagat cgccaagctt gcgcagggaa
     1621 ctgaacgtca gggcatgacc attgtgccgc taaaaatcta ttttaatgaa aagggcaggg
     1681 cgaaaatcga gatcgccctc gcccgcggaa agcagctgca cgacaagcgc gagaccgaaa
     1741 aggcgcgcga ttggaaccgg gaaaaagggc ggctcttgcg ggagaagggg tgaagtgtcg
     1801 gtgacggcga tgcgccaaag agccgaacca aatcaatttg cccagtttct ggctacatct
     1861 gctcataaag tttggcgaac catatgactg cgcattaagt gtttgtaacg aagatgaagc
     1921 gacgagcggg ttcaaattct aagttcctcg tcactggtcg tgaactgctc agaatcgatc
     1981 acgtcgtact tgcaaaaatc aagaattccg cacctctatt ttgaggccga aaacggcggg
     2041 tttttgagag aagcagccta tcttggacat tccggcgcac ctgcctcatt ccatcgcgcc
     2101 agccaatgtt ctactacagg ccgtactccc agcgaacgtc gaccaaattc cagcgatgcc
     2161 tcttccacaa aaacagcgcg gcgaatatct ctctttgcgg cgtgagtttg aacccacgag
     2221 cgtcagactg gtcattgtcg ctgagtcacc tccgatctca ggcaattact tctacaatcc
     2281 ggatggcgag gtaagcgaac aactgttcaa ggcgttgatg gagcagctcg gcattaag
//
LOCUS       DATMPU010000328         4805 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_551197,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000328 DATMPU010000000
VERSION     DATMPU010000328.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 4805)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4805)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4805
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_551197"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..984)
                     /locus_tag="VNY06_07480"
     CDS             complement(<1..984)
                     /locus_tag="VNY06_07480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008971365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HWX14676.1"
                     /translation="MLLAKTLRSQTFKLALISIAIFGAVVIVLLGYADWSTASYVRSR
                     SDHAIAAELTSLQNAFASAGRSGLIAMIPQRIAEQRLEGGVYLLAGPSFAQLAGNLAD
                     WPSAMKGARGWGNFRPQDGRPLLRMAFETLPDGSHLLVGKDIGGLNEFAQKINIALAL
                     SILLIFVLAGVASVLVTRRTVGRIEAINAASRTIMQGGLGQRIPLRGTHDEWDELAAN
                     LNSMLDRIEALMREVKQVTDNVAHDLRTPLARIRGRLEKAYSKQSDGNYDQSLLADTL
                     VDLDAVLRMFSSLMRISQIEAHDRTNAFRYINLVEIASEVVELFDAAAEEKG"
     gene            complement(971..1663)
                     /locus_tag="VNY06_07485"
     CDS             complement(971..1663)
                     /locus_tag="VNY06_07485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016842578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HWX14677.1"
                     /translation="MAGWRRRILVIEDDRETAEQIVEALAASGYAVDLADDGEDGLDR
                     ARSAEYAVMTIDRMLPDIDGIAVIRSLREEGIVTPALIVSALGEIDERVRGLRAGGDD
                     YLVKPFAFAELLARVEALARRSDRVIKETVLRAGDLELDLVSRSVSRGGQKIDLLPRE
                     FKLLEYLVRNQGQVVPRAMLLQHVWDLHFDPTTNIIDVYVGRVRRKIDAAQAYPLIHT
                     VRGVGFCVRAPG"
     gene            1893..2417
                     /locus_tag="VNY06_07490"
     CDS             1893..2417
                     /locus_tag="VNY06_07490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007614573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX14678.1"
                     /translation="MKNNILGKSNILGCLAAATLGAAIVVATPAMAFRGGGGFGGMHG
                     GFGGGGMHFGGGGFGGPHFGGGGFRGGMVTGRSVFVPGAGRFAGAPFAGQRFAVNRFN
                     NFAFRNRFNNFAFRNRFFFRHHHFRNFAFFGAPFAVGWGWPYSYDYGYGECWRQVWTG
                     YGYNWANLCYNYGY"
     gene            complement(2526..>4805)
                     /locus_tag="VNY06_07495"
     CDS             complement(2526..>4805)
                     /locus_tag="VNY06_07495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012592503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 dependent-methionine synthase
                     activation domain-containing protein"
                     /protein_id="HWX14679.1"
                     /translation="AFDEQGQADTLARRVEICSRAYKILMEEVGFPPEDIIFDPNIFP
                     VATGIEDHDKYGVDFIEAVRQIRSRFPLVHISGGVSNLSFSFRGNEAVRQAMHSVFLY
                     QGIAAGMDMGIVNAGQLPVYAEIGPELREACEDVVLNRRANATEILLALAERFRGQGI
                     EAAEKNLAWREEPVEKRLEHALVNGITEFIETDVEEARKLAAKPLDVIEGPLMAGMNV
                     VGDLFGSGKMFLPQVVKSARVMKQAVAYLLPFMDEEKRKNGGRERSSAGKIVMATVKG
                     DVHDIGKNIVGVVLACNNYEIIDLGVMVPAAKILSVARAEKAGAIGLSGLITPSLDEM
                     CFVAAEMEREGFDLPLLIGGATTSRVHTAVKIHPNYTKGQAVYVTDASRAVGVVQTLL
                     SKSSRAATIETVRAEYAKVAAAHRRSEADKQRVPLARARANAFKPDWAGYEPPRPLFC
                     GTRVFRSYETAELIPYIDWTPFFQTWEMRGRYPAILDDPDKGAAARQLFEDASEMLAR
                     AVAEHWFDPKAVIGFWPANSMGDDIKLYTGESRSSELAAFFTLRQQLARRDGRPNLAL
                     ADFIAPVETGKADYIGCFVVTAGAQEGKIADRFAKANDDYRSIMVKALADRIAEAFAE
                     RMHERVRREFWAYAPDEKLTDAERISEAYRGIRPAPGYPAQPDHTEKATLFRLLEAER
                     RIGVILTESYAMWPGASVSGLYLAHPEAHYFGVAKIERDQAEDYARRKGMSIAEVERW
                     LAPVLNYNPASLTQAAAAE"
BASE COUNT          974 a         1463 c         1357 g         1011 t
ORIGIN      
        1 gcccttttcc tccgctgcag cgtcgaatag ctccacgacc tcgctcgcaa tttcaacgag
       61 gtttatgtag cggaacgcgt ttgttcgatc atgcgcctct atttgagaga tccgcatgag
      121 cgaggagaac atgcgaagga cagcatcgag gtcaaccagg gtgtcggcga gcagcgattg
      181 gtcatagtta ccgtcgcttt gcttgctata ggccttttcc agacggccgc gaatccgggc
      241 caaaggtgtg cgcaaatcat gcgccacatt gtccgtgact tgcttcacct cccgcatcaa
      301 cgcttcgatg cggtcgagca tcgaattgag gttcgccgcc agttcgtccc attcatcgtg
      361 tgtcccgcgc aagggtatcc tctggccgag accaccttgc atgatggtac ggctggctgc
      421 gttgatggct tcaatgcgcc ccaccgtgcg ccgcgtgacg agcacgctag ccaccccagc
      481 aagcacgaat atcaacaaga tgcttagggc tagcgctatg ttgatctttt gcgcgaactc
      541 gttaaggccg ccaatgtctt ttcccaccag caaatggcta ccgtcaggca gtgtctcgaa
      601 cgccatccgc agcaggggcc ggccgtcttg cggtctgaaa ttgccccatc ctctggcacc
      661 tttcatggcc gatggccagt ccgcaagatt gcctgcgagc tgagcaaacg atggcccggc
      721 gaggaggtaa acgccccctt cgaggcgttg ttcagcgatc cgttgcggga tcatagcgat
      781 cagtccgctg cgccccgcgc tggcgaaggc attttgcagg ctcgtgagtt ccgccgcgat
      841 cgcatgatcc gatcggctgc gcacgtagga agccgttgac caatcggcgt agcccaataa
      901 cacaatgacc acggcgccga aaatcgctat ggagatcagt gccagcttga acgtctggga
      961 ccgtagggtc ttagccagga gcacggacac aaaacccaac gccgcggacg gtatgaatga
     1021 gtggataggc ttgcgcggcg tcgatcttgc ggcggacacg tccaacataa acgtcgatga
     1081 tgttggttgt gggatcgaaa tggaggtccc atacgtgctg cagcagcatc gcccgcggga
     1141 caacctggcc ttggttgcga accaaatact cgagaagctt gaattccctt ggcagcaagt
     1201 cgattttctg accaccccgg cttaccgatc gtgagaccag gtcgagctcg agatcaccgg
     1261 cgcgcaacac cgtttctttg atgactcggt cgctgcgccg ggccagcgcc tcgacacggg
     1321 ccagcaattc cgcgaaggcg aaaggtttga cgagatagtc gtcgccgccg gcgcgcaggc
     1381 cgcgcacccg ttcatcaatc tcgccgagcg cactgacgat caaggcgggc gtgacgattc
     1441 cctcttccct caggctgcgg atgacggcga ttccatcgat gtctggaagc atgcgatcga
     1501 tcgtcatcac ggcatattcg gcagaacgtg cgcggtccag tccgtcctcg ccgtcgtcag
     1561 caagatcgac cgcatagccg ctcgcggcaa gcgcctccac gatctgctcg gccgtctcgc
     1621 ggtcatcctc gatcacaaga atccggcgcc gccagcctgc catgcctaag catctccaaa
     1681 gcagtgccaa aaagcgttcg tcccgatcag tcttgtcata aaattgaaaa cccaaaaact
     1741 gacttttctg tcagaaactg tcgcattagt catttgagta ctttggttag attgtttatc
     1801 cagatcttac ccggcgtttc tcgccgtcgt gatcccgttt gcggcaatgc ccggcaatca
     1861 ggatgaattc cttcggtgga ggatttggtg tcatgaaaaa caatatcctc ggtaaaagta
     1921 atatcctcgg ttgccttgcc gcggccacgc tgggggcggc aatcgttgtt gcaacgccag
     1981 ccatggcatt ccgtggtggc ggcggatttg gtggcatgca cggcgggttc ggcggtggtg
     2041 gaatgcattt cggcggcggc ggcttcggtg gaccgcattt cggtggcggc ggcttccgcg
     2101 ggggcatggt caccggccgg agtgttttcg tacccggagc aggacgcttt gctggcgcac
     2161 cctttgccgg ccagcgtttc gccgtcaaca gattcaacaa cttcgcattc cggaacaggt
     2221 tcaacaactt cgcgttccgg aaccgcttct tcttccggca tcaccatttc cgcaactttg
     2281 cgttctttgg tgcgcctttt gccgtgggtt ggggctggcc gtactcttac gactacggct
     2341 acggcgagtg ctggcgccag gtatggaccg gttacggata caattgggcc aacctctgct
     2401 ataattatgg ttactgaaga gcctcgagct tgcctgacgc gcaccggcta gccgaaactt
     2461 cgtgtgcagt tctggctcaa gatcgatcgc caggacggcc gctacccgga tgaggctaac
     2521 gccgctcact ccgccgccgc tgcttgcgtg agcgaagccg gattgtagtt caaaactggc
     2581 gcgagccagc gttcaacctc cgcaatgctc atgcctttgc gccgcgcata atcctcggcc
     2641 tgatcgcgct cgatctttgc cactccaaaa taatgcgcct cgggatgcgc gagatagagg
     2701 cccgacacgg aggcgccagg ccacatggcg tagctctcag tcagaatcac gccgatcctg
     2761 cgctccgctt cgagcagacg gaacaaggtc gccttttccg tatgatccgg ctgcgccgga
     2821 tagccgggcg ccgggcgaat cccacgatag gcctcgctta ttcgttcggc atcggtcaat
     2881 ttttcatcgg gcgcgtaggc ccagaactcg cgccggaccc tttcatgcat gcgctcggca
     2941 aaggcctcgg cgatacgatc ggcaagtgcc ttgaccatga tcgacctgta atcgtcattg
     3001 gccttggcaa aacggtctgc gatcttgccc tcctgtgcgc cagcagtgac aacgaaacag
     3061 ccgatatagt ccgccttgcc ggtttcaaca ggcgcgatga aatcggcaag ggcaagattg
     3121 ggccgcccat cgcgccgggc caattgctgg cgcaaggtaa agaacgctgc gagctcggaa
     3181 gatcgcgatt cacccgtata gagcttgatg tcatcgccca tgctgttggc gggccagaaa
     3241 ccgatgacgg ccttgggatc gaaccaatgt tccgcaacgg cgcgcgcgag catttcgctt
     3301 gcgtcctcga acaattgccg tgcggccgcg cctttgtcgg ggtcatccaa aatcgccgga
     3361 tagcggccgc gcatctccca cgtctggaaa aacggcgtcc aatcgatata ggggataagc
     3421 tcggcggtct catagctgcg aaacacgcgc gtaccgcaga acaaagggcg cggcggctca
     3481 tagcctgccc agtcaggctt aaatgcgttg gcccgcgccc tcgccaaggg cacccgctgc
     3541 ttgtcggcct cggagcggcg gtgcgctgcc gcgaccttgg catattcagc ccgcaccgtt
     3601 tcgattgtcg ccgcgcggga agacttcgat aaaagcgtct gcacgacgcc gacggcgcgg
     3661 ctcgcgtcag tgacatagac cgcttggccc ttggtgtaat ttggatgaat tttaaccgcc
     3721 gtatgcaccc ggctcgttgt cgcgccgccg atcaagagcg gcagatcgaa gccttcgcgc
     3781 tccatctcgg cggcaacaaa gcacatctcg tcaagcgagg gggtaatcag gccggagagg
     3841 ccgatcgcgc ccgccttctc ggcacgcgcg acgctcaaga ttttcgccgc cgggaccatc
     3901 acgccaagat caattatctc gtaattattg caggcaagca cgacgccgac aatgtttttg
     3961 ccgatatcat gcacatcgcc ctttacggtc gccatgacga ttttgcccgc gcttgaccgc
     4021 tctctgcctc cattctttct cttttcttcg tccataaaag gaagcaaata ggcgacggcc
     4081 tgcttcatca cacgcgcgga tttcaccacc tgcggcagga acattttgcc ggagccgaaa
     4141 agatcaccga caacattcat gccggccatc aaaggccctt cgatcacatc gaggggtttt
     4201 gccgcaagct tccgcgcttc ttcgacatcg gtttcgatga attccgtgat gccattgaca
     4261 agcgcatgtt cgagacgctt ttcgactggc tcctcccgcc aggcaagatt cttttccgcg
     4321 gcctcaattc cctggcccct aaagcgttcg gcaagagcaa ggagaatttc ggtcgcattc
     4381 gcccggcggt tgaggacaac atcctcgcag gcctcgcgaa gttcggggcc gatctcagca
     4441 tagaccggca gctggcctgc gttgacgatg cccatatcca tgcccgccgc gatgccctgg
     4501 tagagaaaga ccgaatgcat cgcctggcgc acggcctcat tgccgcgaaa cgaaaaggaa
     4561 agatttgaaa ccccgccgga aatgtggacg agaggaaatc gtgaccggat ttgccgcacg
     4621 gcctcgatga aatccacgcc atatttgtca tggtcttcga tgccggtcgc aaccgggaaa
     4681 atattcggat caaaaataat gtcttctggc ggaaacccaa cctcctccat caagatttta
     4741 taggcacggg agcaaatctc gaccctccgc gcaagcgtat cggcctggcc ctgttcgtcg
     4801 aaggc
//
LOCUS       DATMPU010000329         2246 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_1798297,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000329 DATMPU010000000
VERSION     DATMPU010000329.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 2246)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2246)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2246
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1798297"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            319..744
                     /locus_tag="VNY06_07500"
     CDS             319..744
                     /locus_tag="VNY06_07500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX14680.1"
                     /translation="MKHVPISLLIFLAPAFAEELPLNPAVTQATIGGTICKPGWTKAL
                     RPPSRVTDQIKRDKLRAAGWTDADKNRFELDYIIPLSLGGAPADPKNFQLEPGTEVGE
                     KEALEACLPRLVYERRLMLDEARKAVWRDWRAALSLCRD"
     gene            1061..1507
                     /locus_tag="VNY06_07505"
     CDS             1061..1507
                     /locus_tag="VNY06_07505"
                     /inference="COORDINATES: protein motif:HMM:NF017088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042314 - bacteriochlorophyll binding
                     [Evidence IEA];
                     GO_process: GO:0019684 - photosynthesis, light reaction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HWX14681.1"
                     /translation="MLKTLVLSASVLAFAGGLAFAETTITREPSTGRSVVTHPSSSLS
                     TDHWLASDVYKASVYDPSEHKIGDVTDLMIDSNGNVTAAIISVGGFLGVGQKDVVIPF
                     KELKVSTRDGKDWLVLNRTKDELKMAPAYNNTGSPREYRGKGGDIR"
     gene            1854..2108
                     /locus_tag="VNY06_07510"
     CDS             1854..2108
                     /locus_tag="VNY06_07510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019859210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3175 domain-containing protein"
                     /protein_id="HWX14682.1"
                     /translation="MTERKHKNQKWSAEVTKHSYALDLEADIFESDDPAKIAHSLKRS
                     AERSNRRKSAPFRSAMSMLTFYIRAPRKIGASLHRANQIQ"
     gene            2124..2246
                     /locus_tag="VNY06_07515"
                     /pseudo
     CDS             2124..2246
                     /locus_tag="VNY06_07515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611178.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
BASE COUNT          476 a          597 c          664 g          509 t
ORIGIN      
        1 ccttgattgg tcattataat tacacggtcg tgacgtggga ttgcggtaca gctcgtctcg
       61 acggcgatcg tctggctcag gcatccgacc ggtgccatat cgacagtggc ttaggtatgg
      121 acctgtctgc acggtggtga aaaccgcgag agctgtttag tatggccgcg agattgtaag
      181 gctcttgctg gcaaggaatc acactcttgc ggcgtaggct gccaacgctt caatgccaag
      241 gtggcgtcta ttcggggaac tcgcgcagat cgcgccgagt ttgtcattgg cgcagatcgc
      301 tatggatttg agtcccctat gaagcatgtt cccatctcct tattgatttt cctcgcgccc
      361 gcatttgccg aagaacttcc cctcaacccg gccgtcacgc aggcgacgat cggcggcaca
      421 atctgcaagc cgggctggac taaggcgctt cgcccgcctt cccgcgtgac ggatcaaatc
      481 aagcgtgaca agcttcgcgc ggccggctgg acagatgcgg acaagaaccg atttgagctc
      541 gattacatta tccctctcag cctcggcggg gcaccggctg atccgaagaa tttccagttg
      601 gagccaggga ccgaggtcgg ggagaaggaa gcgctggagg cgtgcctgcc gcgcttggtg
      661 tacgagcggc gattgatgtt ggacgaagct aggaaagctg tttggcggga ctggcgcgcc
      721 gctttgagcc tttgccggga ttgaggcgtg tggcgcatcg ttgagacatt gcgcaccgtg
      781 gaatttttcc tacatggcgg ccatggcgaa atcaagtggc gacgtgctca ggcttgtatt
      841 tcaaccgccg gctcacggtt cagttcgcag cggtctaccg cgaactcgac gacgcggtcg
      901 gactgaccac gatggccggc gctagccgat gcgcggacgg gccggaatgg ccgtcatgcg
      961 ctcgtcggac ttggcggtag ccggtgggaa ccaagctctg gccacgacgt ttcccgagct
     1021 gactcccgat gtggcgtcgc aatagcgaag gagatctgtt atgttaaaga cgctcgtctt
     1081 gtccgcctcg gtcctcgctt tcgctggcgg tctcgctttc gcagagacca cgatcaccag
     1141 agagccatcg accggacgct cggtcgtgac ccatccttcg tcttcgcttt cgacggacca
     1201 ttggctcgcc tccgatgtct acaaagcaag tgtttatgat ccttctgaac acaagatcgg
     1261 cgatgtcacc gatcttatga tcgatagcaa cggcaacgta acggccgcga tcataagcgt
     1321 cggcgggttc cttggtgttg gccagaaaga tgtcgtaatt ccctttaagg agctgaaagt
     1381 ctcgacgcgc gatggcaagg attggctggt cctcaatcgc accaaagacg aattaaagat
     1441 ggctcccgcc tacaataata caggatcccc gcgcgagtat cggggcaaag gaggggatat
     1501 tcgctgatgc ctggcaacgc attccgttta ttttgatcca cctactaagc atcatgtttg
     1561 gcgtcgtaag aattctgagc cacgagagat gtttggcagt ccattgccta tcgtagctct
     1621 aaggcggtcg tgaatgggca tgctgtgatt gattcggtgg gatctgaggc gcctgtgatt
     1681 agccccgggc cttgatgctc agcctcggag attcgtccgc gtttcctcca gtcctgctca
     1741 gaccggaact aacgacgata aaaaaaagtt gccgcttcgc tctgggcctg ggatgaacga
     1801 aactccgctt aaggggattt gttttcggga cgtcttagcg cgggatttaa gcgatgacag
     1861 agcgaaagca taagaaccag aaatggtcgg cggaagtgac aaagcatagt tatgctctcg
     1921 atcttgaggc ggatatcttt gaaagcgacg atccggccaa gatagcgcac tccctgaaaa
     1981 ggtccgcgga gcgcagcaac cggcgtaagt ctgcgccgtt ccgttctgca atgtcgatgc
     2041 tgacgttcta catcagggca cctcgaaaaa taggcgcttc cctccaccgc gcaaatcaga
     2101 ttcaatgaac gggggaggtt ggcatgggac agcttgggtt cttcgatttg aaccgacgct
     2161 atgaaagtct cattgaaaag aacgatccgc ttgtagcgaa ttgccgcgat ggttccgttc
     2221 gaattcttcc ggccaaagct tgaatg
//
LOCUS       DATMPU010000330         2927 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_245554,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000330 DATMPU010000000
VERSION     DATMPU010000330.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 2927)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2927)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2927
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_245554"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            23..1522
                     /locus_tag="VNY06_07520"
     CDS             23..1522
                     /locus_tag="VNY06_07520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012591797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HWX14683.1"
                     /translation="MFPKPVAQLATNTYAYESSPMVKATGFREYDARWLFEKEINLMG
                     VQALGLGLGTLIHDMGVTPEVVTGHDFRSYSASIKQALTTGLMAAGMKVHDIGLALTP
                     MAYFAQFDLDIAAVAMVTASHNDNGWTGVKIGAKRPVTFGPDEMGRLKDIVLQGQFRY
                     ADRGSYRFVEDFPARYIADLTNRPKISRKLKVVAACGNGTAGAFAPKVLEMLGCEVVP
                     LDTNLDFTFPHYNPNPEDLHMLHAMAEKVRESGADIALGFDGDGDRCGVVDNRGEEIF
                     ADKVGVLLARDLSKSHPGSTFVCDVKSTGLFATDPVLLENGAKADYWKTGHSYIKRRV
                     SELKALAGFEKSGHYFFNAPIGRGYDDGIVAAIHILDMLDRNPQKSMAELYEALPKTF
                     GSPTMSPHCDDEKKYAVVDRVTERFKTMQAKGELLIGQKIRDIVTVNGVRVTLEDGTW
                     GLVRASSNKPELVVVVESPVSDARMREIFAAIDKVLRENPEVGAYNQTI"
     gene            2024..>2927
                     /locus_tag="VNY06_07525"
     CDS             2024..>2927
                     /locus_tag="VNY06_07525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015343775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX14684.1"
                     /translation="MAVEIIVHPGVDSAFIAWRAPFIDQCRGFALYRKVKRGPGSAAS
                     PNTTSSANAQGFAEEIVASWVGFADGPDVEPGTRKPTTEWPIQKYLWSDFMVGPGDEV
                     AYRVTPMIGQSDALKEANDQASPWSPTVAIGAETEGRAACFFNRGIVASQWLTRLLPQ
                     DHPSTKLAKVIASPGDKIRNFLAGPVRDKLVSLLTDAKAAKGHIYAALFELDDPELIP
                     LLQGLRKRAHIVLGNGSVKRKGEDENADARAALHMCDVKDRMTAPRALAHNKFLVICD
                     ADKAPQAVWTGSTNWTKTGLCTQAN"
BASE COUNT          621 a          836 c          859 g          611 t
ORIGIN      
        1 ccctttgacg caaggacctt ccatgtttcc caagcccgtg gcgcaattag cgaccaatac
       61 ttatgcctat gaaagctcgc cgatggtgaa agcgaccggc tttcgcgaat atgacgcgcg
      121 ctggctgttt gaaaaggaaa ttaatctcat gggcgtccag gccttggggc tcggacttgg
      181 cacgctcatt catgacatgg gcgttacgcc ggaagtcgtg acgggacatg atttccgctc
      241 ctattcggcg tcgatcaaac aagcgctgac gactggtctc atggcggcgg ggatgaaggt
      301 tcatgatatt gggcttgcgc tgaccccgat ggcttatttt gcccagttcg atctcgatat
      361 tgcggcggtt gccatggtga ccgcctcgca taatgacaat ggctggaccg gcgtaaaaat
      421 aggcgcgaaa cgcccagtga cttttggccc cgatgagatg gggcgtctga aagacatcgt
      481 tttgcaaggc caattccgtt acgcggaccg cggctcctat cgtttcgtcg aggattttcc
      541 ggctcgctat attgccgatc tcacgaaccg gccgaagatc agccgcaagc tgaaagtcgt
      601 cgcggcctgc ggcaatggca ccgcgggcgc cttcgcgcca aaagtgttag aaatgcttgg
      661 ctgcgaagtc gtgccgctcg acacaaacct tgattttact ttcccgcatt ataatcccaa
      721 ccccgaagac cttcacatgc tccatgccat ggccgaaaag gtgcgcgaat ccggcgccga
      781 tatcgcgctt ggctttgatg gcgacggcga ccgctgcggc gttgtcgata atcgcggcga
      841 agagattttt gccgataagg tcggggtctt gctcgcccgc gacctttcaa agtcccaccc
      901 gggttcgaca ttcgtgtgcg atgtcaaatc cacgggactc tttgccaccg atccggtgct
      961 tctcgaaaat ggtgccaagg ccgattattg gaagacgggg cattcctata tcaagcggcg
     1021 ggtgagcgaa ctcaaggcgc tcgcgggctt tgaaaaatcc gggcattatt ttttcaatgc
     1081 tccaatcggg cgcggctatg atgatggcat tgtcgccgcc atccatattt tggacatgct
     1141 cgaccgcaat ccgcaaaagt cgatggccga gctttacgag gcgttgccca agacttttgg
     1201 ctctccaacc atgtcgcccc attgcgacga cgaaaaaaaa tatgccgtgg tcgacagggt
     1261 gaccgagcgt ttcaaaacca tgcaggccaa gggcgagctc cttatcggcc aaaagattcg
     1321 cgatatcgtc accgtcaatg gcgtgcgggt gaccctcgag gatggcactt gggggctggt
     1381 ccgcgcctcc tcgaataagc cggaactcgt ggtcgtcgtc gaaagcccgg tatctgacgc
     1441 ccgaatgcgc gagatttttg ccgccatcga caaagtcttg cgcgagaacc cggaggttgg
     1501 ggcttacaat cagacgatat agggcccggc ggccaccgcc gaccaatctt acaaaaatgt
     1561 ttcacgtgaa acatttttgt ccgatggggg cccaaaacct tacaaggcgc aagacagcgg
     1621 tgccttctta gctcttgtaa aatcgtgcga ttttttggtg caattaaaat cgggcggcag
     1681 tgcgcgttga cggcccagcc gatcgccaga aggcatcccg gtgtaaggtt cgctgacagg
     1741 ctgggcctga attcggcaaa aaattcttgg ctttcgcacg gcgatggcgc tgctttcgaa
     1801 catgtccgca gctgaatgcg ctacggacac caaagccccc ttagctcgac agccggtttt
     1861 gacctaactc ggacattgac ggtcgccaaa ggtggatcca ggcgttggat cggctgaagc
     1921 tggaaaggtt gtcccttcga accaagccat gtttctttcg ttgacagtca gtaaagcgcg
     1981 agcataacta acttaatcat gcaaattgcg gcgaggtcgt ccgatggccg tcgagatcat
     2041 cgtccacccg ggtgtggata gcgccttcat cgcgtggcgc gctccattta tcgaccagtg
     2101 ccggggcttt gccctatatc gcaaagtaaa gcgcgggcca gggagtgccg cgagtccgaa
     2161 taccaccagc agcgcgaatg ctcagggttt tgctgaagag atcgtcgcaa gctgggttgg
     2221 cttcgccgat ggacctgatg tggagcccgg cacccgcaag ccgacgaccg agtggccgat
     2281 tcagaaatat ctctggtcgg acttcatggt gggccctggc gacgaggtcg cttatcgcgt
     2341 tacgccgatg ataggtcagt cagatgcgct caaggaggcg aacgaccagg cctccccttg
     2401 gagccccaca gtcgcgatcg gtgctgaaac cgaggggcgt gcggcctgct tcttcaatcg
     2461 cggcatcgtc gctagccagt ggctcacccg cctgctgccg caggaccatc cttcgacgaa
     2521 gcttgcgaag gtgatcgcct cgccgggcga caagatccgg aatttcctag caggaccagt
     2581 ccgcgacaag ttggtctcgc tgctcaccga cgccaaagca gcgaaggggc atatctatgc
     2641 cgccttgttc gagctcgacg atcccgagtt gatcccgctg ctgcaagggc tccgcaagcg
     2701 tgcgcacatc gtgcttggca atggcagcgt caagcggaag ggcgaggatg agaacgccga
     2761 tgcgcgggcg gcgctccaca tgtgcgacgt gaaggaccgc atgacggcgc cgcgcgcgct
     2821 cgcgcacaat aagttcctcg tgatctgcga cgccgacaag gcgccgcaag ccgtctggac
     2881 cgggagcacc aattggacca agaccggcct ctgcacccag gcgaaca
//
LOCUS       DATMPU010000331         4566 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_4580893,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000331 DATMPU010000000
VERSION     DATMPU010000331.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 4566)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4566)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4566
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4580893"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..355
                     /locus_tag="VNY06_07530"
     CDS             <1..355
                     /locus_tag="VNY06_07530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012384093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HWX14685.1"
                     /translation="TIRALRFYEDRGLLRPRREGSVRWYDDRDRLRLKMILRGKLLGF
                     TLAEINDVLAGRGEENGKAELELELTPGQIAAQISHLEQQRSQIEEAIATLREAQQRL
                     LEVQSSEKRSDRSDP"
     gene            697..2490
                     /locus_tag="VNY06_07535"
     CDS             697..2490
                     /locus_tag="VNY06_07535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018260382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HWX14686.1"
                     /translation="MPSYKAPVEEALFLLSDVFPIQRYNNLPGFADATQDVVEAIVSE
                     AAKLCEETLQPLNQLGDRAGCVRNGDGSVSTPRGFKEAYRAFTDGGWIGLAADPNFGG
                     QGLPYSLAAVVNEFVSSANMAFGMYPGLSQGALNALLTNGTEEQKKLYVPKLISGDWS
                     GTMNLTESHCGTDLGLLKTRAVPNGDGSYAISGEKIFISAGEHDLAENIIHLVLARID
                     GAPAGVKGISMFVVPKILVAPDGAPGSANKVSCGSIEHKMGIHANSTCVMNYDGAWGS
                     LIGEPNKGLNAMFVMMNEARLGVAIQGLALSEVAYQNAAAYAKERLQGRSLSGPKYPE
                     KPADPIIVHPDVRRNLLEIRAFNEAARALVLWTAHKSDIARRSNGKAEAQAAADSLGL
                     ITPLLKGVLTDRGFDNTVKAQQVLGGHGYIEEWGMAQFVRDARVAMIYEGANGIQALD
                     LVGRKLPKDGGRALTAFLAEVKSFIKDNESNASLSPYLKGLAQGLAHLEQATLWFMQN
                     AIAKPDNAGAGSYDYMHLLGLVALAYMWALIAKAAIAKKAAANGDAQTMETKLLRAKF
                     FMEHMMPETSLRLARISAGAGTMMAIGAEQF"
     gene            2537..3745
                     /locus_tag="VNY06_07540"
     CDS             2537..3745
                     /locus_tag="VNY06_07540"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019902991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HWX14687.1"
                     /translation="MPEAFIYDAIRTPRGRGKPDGALHEVSTLGLATTVLRAIKDRAG
                     LDPEFVDDVVLGCVDPVSEAGGDIARAAALAADYGDHVPGVQINRFCASGLDAVNFAA
                     AQIMAGQHELTIGGGVESMSRVGIGASGGAWPVDPAIAIKAYFMPQGISADLIATKYG
                     FSRDDVDQYAVSSQQRAAKAWSEGRFKNSIVPVRDVNGITLLDRDEHMRPSTDMQSLA
                     ALKPSFVMTGEQGGFDAVAVDAHPEIEKVLHVHHAGNSSGIVDGAAAVLIGSSEAGNA
                     AALKPRAKIRAFANIGSEPAMMLTGPVDVTKKVLARAKMTLADIDLLEVNEAFASVVL
                     RYIQAFDLDTSKLNVNGGAIAMGHPLGATGTMLLGTALDELERTGKATALIALCIGAG
                     MGTATIIERV"
     gene            3767..>4566
                     /locus_tag="VNY06_07545"
     CDS             3767..>4566
                     /locus_tag="VNY06_07545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020091386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HWX14688.1"
                     /translation="MNLKNFRFETDNDGIALLTWDMPGRSMNVITPEVIDEIENTVDH
                     VAAQTGIKGCVIASGKETFSGGADLSMLQNAAADYQKARQEKGEEEATKLFFERASRL
                     SLVYRKLETCGKPFAIAISGICLGGAFELALACHYRVIADDDKTKVGLPEIKVGLFPG
                     GGGTQRVARLMPTADALQMLFKGDQIKPKAAKTMNLVHEVTPAAEIVAKAKAWIAAGG
                     KAGSPWDEPNFRLPSGKVFSPQGMMMWPPANAIYRPETHDNYPAAKAI"
BASE COUNT         1004 a         1281 c         1358 g          923 t
ORIGIN      
        1 cacgatcagg gcccttaggt tttacgagga ccgggggttg ctgcgcccga gacgggaagg
       61 cagcgtccgg tggtatgatg atcgcgaccg gctccgcctt aagatgattc ttagaggcaa
      121 gctgctgggt ttcaccctgg ccgagataaa cgatgtattg gcgggccggg gcgaggagaa
      181 tgggaaggcc gaacttgagc tggaactgac gccaggacag atcgctgcgc aaattagcca
      241 tctcgaacag cagcgcagcc aaatcgagga ggcgattgcg acccttcggg aggcgcagca
      301 gcgtctgctc gaagtgcaaa gcagcgaaaa acgttctgat cgatccgacc cttaaaagcg
      361 ggaagcggtt tgcgcccggt cacacgataa aactagaatc tagggcaaga gaacgattcg
      421 agaataagtc accctgctcc agagaacctt aaggtcagcg aagcgaagtc gcccaacgcg
      481 cccagatcga atcaattgcc cttcgcgtca atcggtgata gcggaccaat atcgggtgca
      541 catgacccat gaatgcacct caaatcattt ctgtcccttg ttgagagcgc gcttgtgcgg
      601 ggttcgttcg gccaacgccc aattgctgga cgcctcgcca ctgactatat aaaccgcgat
      661 ccaaggcgcc ggctgcgacg caccgggaga ttattcatgc cgagttacaa ggcccctgtc
      721 gaggaagccc ttttcctcct aagcgatgtt tttccaatcc agcgttacaa taatttgcct
      781 ggctttgccg atgccacgca agacgtcgtc gaagcaatcg tttctgaagc ggcgaagctt
      841 tgcgaggaaa cccttcagcc tctcaatcag cttggcgacc gcgcgggatg cgtgcgaaat
      901 ggggatggct ccgtttcaac gccgcgcggg tttaaagagg cctatcgcgc ctttaccgat
      961 ggtggatgga tcggccttgc ggccgatccc aactttggcg ggcaaggtct gccctactcg
     1021 cttgccgcgg tcgtcaatga attcgtcagc tccgccaata tggcatttgg gatgtatcct
     1081 ggcttgagcc aaggcgcctt gaacgccttg ctgacaaatg gcacggaaga gcagaagaaa
     1141 ctttacgtgc caaaactgat cagcggcgat tggtcgggga caatgaactt gacggaatcg
     1201 cactgcggca ccgatctcgg ccttctcaag accagggccg tccccaatgg cgatggctct
     1261 tatgcgattt ccggcgagaa gatatttatt tccgcgggtg agcacgatct cgcggaaaac
     1321 atcatccacc tcgtgcttgc ccggatcgat ggcgcgcctg ctggcgtcaa gggcatttcg
     1381 atgttcgtgg tgcctaagat ccttgtcgcg cccgatggtg cgcccgggtc tgccaacaaa
     1441 gttagctgcg ggtcgatcga gcataagatg ggcattcacg ccaattcgac atgcgtgatg
     1501 aattatgatg gcgcctgggg ctctctcata ggcgagccga ataagggcct caacgccatg
     1561 tttgtgatga tgaacgaggc caggctcggc gtcgcaattc aaggattggc attgtcagaa
     1621 gtggcctatc agaatgctgc cgcctatgcc aaggagcgcc ttcagggccg ctcgctctcc
     1681 gggcccaaat atcccgaaaa gcctgccgat ccgatcatcg tccaccccga cgtgcgccgc
     1741 aatctcctgg agatccgcgc tttcaacgaa gcggcgcggg ctcttgtgct ttggaccgcg
     1801 cacaaaagcg acatcgcccg tcgctccaac ggcaaggccg aagcccaggc cgcggccgat
     1861 agtctcgggc tgatcacgcc gctgctcaaa ggcgtgctga ccgacagagg cttcgataac
     1921 acggtcaagg cccagcaagt cctcggcgga cacggttata tcgaggaatg gggcatggca
     1981 caattcgtcc gcgatgcgcg cgtagccatg atctacgaag gcgccaatgg cattcaggcg
     2041 ctcgaccttg tcggccgcaa gctcccaaag gacggcggcc gcgcgctcac cgccttcctt
     2101 gccgaagtca aatctttcat caaggacaac gaatccaacg cgtccctttc gccctatctc
     2161 aaaggccttg cccaagggct cgcacatctc gagcaggcaa cattatggtt tatgcaaaac
     2221 gccattgcca aacccgataa tgcgggcgcg ggctcttatg attatatgca tctcttaggg
     2281 cttgtggcgc ttgcctatat gtgggcgctc atcgccaagg ccgcgattgc gaaaaaggct
     2341 gcggccaatg gcgatgcgca aacgatggag acgaagctgc tgagggccaa atttttcatg
     2401 gagcatatga tgccagagac aagcctccgc cttgcccgca tttccgcagg tgccggaacc
     2461 atgatggcga tcggagcgga gcaattttga tcttttcgcg cgcggcgcgg caaatcaaac
     2521 cagaccggag gattatttgc ccgaagcttt catttacgat gccattcgta ccccgcgcgg
     2581 ccgcggcaaa ccagacggcg cgctgcacga agtttcgact cttggccttg ccacgaccgt
     2641 gctgcgtgcc atcaaggatc gtgcgggcct agatccggag ttcgtcgacg atgttgtgct
     2701 aggctgcgtt gatcctgtca gcgaagcggg cggcgacata gcccgcgcgg cggcgcttgc
     2761 tgcagattac ggcgaccatg tgccgggcgt gcagattaac cgattttgtg cctctggcct
     2821 cgacgcggtc aattttgccg ctgcgcaaat catggcggga cagcatgagc ttacgatcgg
     2881 cgggggcgtc gaatctatga gccgtgtcgg catcggcgcc tcgggtgggg cttggccggt
     2941 tgatcctgcg atagcgatca aggcctattt catgccgcaa gggatttccg ccgatctcat
     3001 tgcgacaaaa tatggatttt cccgcgacga tgtagatcag tacgcggttt cctcacaaca
     3061 acgcgcggca aaggcatgga gcgaggggcg tttcaaaaat tcaattgtgc cggtgcgcga
     3121 cgtcaatggc atcacgctgc ttgatcgcga tgagcatatg cggccttcga ccgatatgca
     3181 gtcgctcgct gccttaaaac cttcattcgt gatgaccggc gagcagggcg gctttgatgc
     3241 ggtcgctgtc gacgctcacc ccgaaatcga gaaagtcttg catgtgcatc atgccggtaa
     3301 ttcgtcggga attgtagatg gcgcggcggc tgttctcatc ggctcgagcg aagcgggtaa
     3361 tgcagcggct ctaaagccgc gggcaaaaat tcgcgctttt gctaacattg gctcggagcc
     3421 cgcaatgatg ctgacgggtc cggtggacgt tacaaagaag gttctggccc gggcgaaaat
     3481 gaccctcgcc gacattgacc tccttgaagt caatgaggca tttgcctccg tggtgttgcg
     3541 ctacatccag gccttcgatc tcgacacgtc aaagctaaat gtcaatggcg gtgcgattgc
     3601 aatgggacat ccgcttggcg cgaccggcac tatgctgctt ggcaccgcgc tcgacgaact
     3661 cgaacgaaca gggaaggcca cggcgctcat tgcgctttgt attggcgccg gcatgggcac
     3721 tgcgacgatc atcgaacggg tgtgagagaa ggcgcgggga agatcgatga acctcaagaa
     3781 tttccgcttt gagacggaca atgacggcat cgccttgctg acttgggata tgccagggcg
     3841 gtcgatgaat gtcattacgc cggaggttat cgacgaaatc gaaaataccg tggatcacgt
     3901 ggcggcgcag accgggatca agggctgcgt tatcgcctca ggcaaggaaa cattttcagg
     3961 tggcgccgat ctttccatgc tgcaaaacgc cgccgccgac tatcaaaagg cgcggcagga
     4021 aaaaggcgaa gaagaagcca caaaactctt tttcgaacgc gcaagccgac tatcgctggt
     4081 ttatcgcaag ttagagacct gcggcaagcc gttcgcgatc gcaatcagcg gcatttgtct
     4141 tggcggcgca tttgagcttg cgctcgcctg ccattaccgg gtcattgccg acgacgacaa
     4201 gacaaaggtc gggctgccgg aaatcaaggt cgggctcttt ccgggcggag gtggaacgca
     4261 gcgcgtcgcc cgcctgatgc cgaccgccga tgcgctccag atgcttttta agggggatca
     4321 gatcaagccg aaagcggcaa aaacaatgaa cctcgtgcat gaggtcacgc ccgcggcaga
     4381 aatcgtcgcg aaggccaagg cctggatagc ggcgggcgga aaggcaggtt ccccttggga
     4441 cgaaccgaac tttaggcttc cttccggaaa agtattctcg ccccaaggca tgatgatgtg
     4501 gccgccagcc aatgcgatct accggcccga gacccatgac aattatccgg cggcaaaggc
     4561 gatttt
//
LOCUS       DATMPU010000332         9089 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_3356052,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000332 DATMPU010000000
VERSION     DATMPU010000332.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 9089)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9089)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9089
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3356052"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..173)
                     /gene="rplW"
                     /locus_tag="VNY06_07550"
     CDS             complement(<1..173)
                     /gene="rplW"
                     /locus_tag="VNY06_07550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012384353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HWX14689.1"
                     /translation="MSPSKLAHDTQHYDIILAPVITEKATMASEANQVIFKVRKNATK
                     PQIKAAVERLFDV"
     gene            complement(170..790)
                     /gene="rplD"
                     /locus_tag="VNY06_07555"
     CDS             complement(170..790)
                     /gene="rplD"
                     /locus_tag="VNY06_07555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016917713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HWX14690.1"
                     /translation="MKIDITSFDGENAGSLELNEAIFGLAPREDLIARMVRYQLAKRR
                     AGTHQTLGRSNIARTGKKLYKQKGTGSARHGSARVSQFRGGGRAFGPVVRSHAHDLPK
                     KLRVLALKHALSAKAKEGQIMVFAEVKSTDAKTKSLRTSLQKLGLTNALIIDGTEVAT
                     NFQLAARNIPQIDILPVQGINVYDIIRREKLVLTKAAIKVLEARFK"
     gene            complement(787..1512)
                     /gene="rplC"
                     /locus_tag="VNY06_07560"
     CDS             complement(787..1512)
                     /gene="rplC"
                     /locus_tag="VNY06_07560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014890763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HWX14691.1"
                     /translation="MRSGVIAQKVGMTRVFTAAGEHVPVTVLRLDGCQVVAHLTKDRN
                     GYTAVQLGIGRAKVKNVSKAERSRFAVAKVEPKLKLAEFRVEEDALLPVGAEITADHF
                     IVGQFVDVTGTSTGKGFAGAMKRWNFGGLRASHGVSISHRSHGSTGGRQDPGKTFKNK
                     KMAGHLGTERVTTLSLRVVETDTERGLILVEGAVPGTSGSWIYVRDAVKKALPKEAPK
                     PGKFRIAEAASGDAAGEQTEAGA"
     gene            complement(1589..1897)
                     /gene="rpsJ"
                     /locus_tag="VNY06_07565"
     CDS             complement(1589..1897)
                     /gene="rpsJ"
                     /locus_tag="VNY06_07565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005773267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HWX14692.1"
                     /translation="MNGQNIRIRLKAFDHRILDSSTKEIVSTAKRTGAQVRGPIPLPT
                     RIEKFTVNRSPHVDKKSREQFEMRTHKRVLDIVDPTPQTVDALMKLDLAAGVDVEIKL
                     "
     gene            complement(2010..3200)
                     /gene="tuf"
                     /locus_tag="VNY06_07570"
     CDS             complement(2010..3200)
                     /gene="tuf"
                     /locus_tag="VNY06_07570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008963462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HWX14693.1"
                     /translation="MGKEKFARTKPHCNIGTIGHVDHGKTTLTAAITKVLAETGGATF
                     TAYDQIDKAPEEKARGITISTAHVEYQTQKRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLARQVGVPALVVYLNKVDMVDDSELLELVELEVRELLSK
                     YDFPGDDIPITRGSALFALEGRSPEIGHDSVLKLMESVDSYIPQPERPIDQPFLMPVE
                     DVFSISGRGTVATGRVERGAVKVGEEVEIVGLRPTTKTTVTGVEMFRKLLDQGQAGDN
                     IGALLRGTKREEVERGQVLCKPGSVKPHTQFKAEAYILTKDEGGRHTPFFTNYRPQFY
                     FRTTDVTGVVTLPEGTEMVMPGDNITMDVELIAPIAMEEKLRFAIREGGRTVGAGVVS
                     AIKE"
     gene            complement(3244..5319)
                     /gene="fusA"
                     /locus_tag="VNY06_07575"
     CDS             complement(3244..5319)
                     /gene="fusA"
                     /locus_tag="VNY06_07575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004447767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HWX14694.1"
                     /translation="MPRSHPIEDYRNFGIMAHIDAGKTTTTERILYYSGKSHKIGEVH
                     DGAATMDWMEQEQERGITITSAATTTFWRGKRLNIIDTPGHVDFTIEVERSLRVLDGA
                     VCVLDGNQGVEPQTETVWRQADKYNVPRIVFVNKMDKIGADFYRCVEDLKTKVGARPI
                     GIQLPLGAESDFRGIIDLVRMKAVVWEDEALGANYADAEIPEDLKAKADEYRHMLVEA
                     AVELDDDVMAAYLEGTEPDTEILKRLLRKAVREIAFIPVLCGSAFKNKGVQPLLDAVV
                     DFLPSPRDREAIKGTDMETGAEVLRLPRDEDPFSMLAFKIMDDPFVGTITFARVYSGH
                     VESGTTVLNSTKEKKERIGRMLLMHANNREDIKEAYSGDIVALAGLKDTRTGDTLCDL
                     NKPVVLERMEFPDPVIEIAIEPKSKADQEKLGVALAKLAAEDPSFRVSTDQESGQTIL
                     KGMGELHLDIKVDILRRTYKVDANIGAPQVAYREKMTRRVEIDHTHKKQTGGSGQFAR
                     VKIVFEPTEAGIGSGFESQVVGGAVPKEYIPGVEKGIESVMGSGVLAGFPVVDVKATL
                     IDGAYHDVDSSVLAFEIAARAAFREALQKGGCVLLEPIMKVEVTTPEDYTGSVMGDLL
                     GRRGHVHGQDMRGNAVVINAMVPLANMFGYVNQLRSMSQGRANYTMQFDHYEQVPAGE
                     AGKIQAKYA"
     gene            complement(5362..5832)
                     /gene="rpsG"
                     /locus_tag="VNY06_07580"
     CDS             complement(5362..5832)
                     /gene="rpsG"
                     /locus_tag="VNY06_07580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012589626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HWX14695.1"
                     /translation="MSRRHRAEKRDIIPDAKFGDIVVTKFMNSIMHDGKKSIAETIVY
                     GAFDSIEQKSRVEPLAIFKQALDNVAPSIEVRSRRVGGATYQVPVEVRSERRQALAIR
                     WIISAARGRNDKTMVDRLSAELMDAANNRGNAVKKREDTHRMAEANRAFSHYRW"
     gene            complement(5939..6310)
                     /gene="rpsL"
                     /locus_tag="VNY06_07585"
     CDS             complement(5939..6310)
                     /gene="rpsL"
                     /locus_tag="VNY06_07585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020185797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HWX14696.1"
                     /translation="MPTISQLIRKPRQEKSYREKARHLGACPQKRGVCTRVYTTTPKK
                     PNSALRKVAKVRLTNGFEVIGYIPGEGHNLQEHSVVMIRGGRVKDLPGVRYHILRGVL
                     DTQGVKNRKQRRSKYGAKRPK"
     gene            7143..7523
                     /locus_tag="VNY06_07590"
     CDS             7143..7523
                     /locus_tag="VNY06_07590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX14697.1"
                     /translation="MAWRSNPILSETDIVLNSLKFLPVSGAADFRDMLVAKAESPPKA
                     PKPTTLEQFIATHPTVPLALAMAQNPRQFRRRRLSGINCIEKRPVRYIAKPEKLVHIE
                     PTEAAKMAPDLFDGAGGASMPEAP"
     gene            complement(7921..8271)
                     /locus_tag="VNY06_07595"
     CDS             complement(7921..8271)
                     /locus_tag="VNY06_07595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX14698.1"
                     /translation="MIELLIAGGALLGAIIGRFFKVLVLIPVCAVAAALQWMNCQMAE
                     KTVLDSLASVGLLILSLEFGYFTGLLSTDIFAAAHRLSEFRARLRHESQARQAPPEVG
                     VNLANYLGRREIKS"
     gene            complement(8399..8554)
                     /locus_tag="VNY06_07600"
     CDS             complement(8399..8554)
                     /locus_tag="VNY06_07600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX14699.1"
                     /translation="MDPAKLSALGHGEAKEVEVKATIDGTAMGGIVALAGLPKALAWL
                     NFECGQK"
BASE COUNT         1884 a         2722 c         2478 g         2005 t
ORIGIN      
        1 ttgacgtcaa agagacgttc gactgccgcc ttgatctgtg gcttcgtcgc gttctttcga
       61 accttgaaga tgacctgatt tgcctccgac gccatcgtcg ccttttcagt gataaccggg
      121 gcgagaatga tgtcgtaatg ctgggtgtca tgggcgagtt tggaagggct catttgaaac
      181 gcgcctccaa gactttgatc gcggctttgg tcaagacgag cttctcgcgg cggataatat
      241 cgtagacatt gatgccctgc accggcagga tgtcgatttg cggaatgttg cgcgcagcaa
      301 gctggaagtt cgtcgccacc tcggtgccgt ctattatcag cgcattggtg aggccgagct
      361 tttgcaagct cgttctcaaa gatttggtct tggcatctgt cgacttcacc tcggcaaaga
      421 ccatgatttg gccttccttt gctttggcgg aaagcgcatg tttgagggcg agcacgcgca
      481 gctttttggg cagatcatgg gcatgcgagc gcacgaccgg accaaaggcc cgcccgccgc
      541 cgcgaaattg ggacacacgc gcagatccat gccgcgccga accggtcccc ttctgcttgt
      601 atagtttctt gccagtgcgc gcgatgttgg agcggccgag ggtctgatgc gttccggcgc
      661 ggcgtttggc gagttggtag cgcaccatgc gcgcaatcaa atcctcgcgc ggcgcaagac
      721 caaagatcgc ctcattgagc tcaagagagc cggcgttttc accgtcgaag gaggtgatgt
      781 cgattttcac gcgccggcct ccgtctgttc gcccgctgcg tcaccagagg cggcctccgc
      841 aatgcggaat tttccgggct ttggcgcctc cttcggaagc gcctttttca cggcatcgcg
      901 gacataaatc cagcttccag acgtccctgg gaccgcgccc tcgacgagaa taaggccgcg
      961 ctcggtatcg gtttcaacga cgcgcaaact caacgtcgtt acccgctcgg tgccaagatg
     1021 acctgccatt ttcttgttct tgaaggtctt gcccggatcc tgcctgccgc cggtcgaacc
     1081 atgcgaccgg tgggagatcg acacgccatg cgacgcccgc aagccgccaa agttccagcg
     1141 cttcattgcg ccggcaaaac ccttgcccgt gctcgttccc gttacatcga cgaactgacc
     1201 gacaatgaaa tgatccgccg tgatttcagc gccaactgga agcagcgcat cttcctccac
     1261 gcgaaattca gctagcttca attttggctc gactttggca accgcgaacc ggctgcgttc
     1321 cgccttagat acgttcttga cctttgcccg cccaatacca agttggaccg cagtgtagcc
     1381 gttacggtcc ttggttaggt gcgccacgac ctgacagcca tcgagcctta agaccgtcac
     1441 cggcacatgc tccccagcgg ccgtaaaaac gcgggtcatg ccgacctttt gtgctatgac
     1501 acctgaccgc atcattaact tcctctaccg ccgcggcccg gaaatccgcc gcgcaaaact
     1561 ccaattatgc aaagacctga agcgcacgct aaagtttgat ctcgacatcg acgccggcag
     1621 caagatcgag cttcatcaaa gcgtcaaccg tctgcggcgt cggatcgaca atgtcgagaa
     1681 cgcgcttatg ggtcctcatt tcaaactgtt cgcgcgactt cttatcgaca tgcggcgaac
     1741 ggttgaccgt gaatttttca attctcgtcg gcagcggaat gggcccgcgt acctgtgctc
     1801 ccgtgcgctt tgccgtcgaa acgatctcct tcgtcgaact atcgaggatt cgatgatcga
     1861 atgcttttaa tcgaatgcga atattctggc cgttcatctc aaccaatccc tgctccgcca
     1921 tgaaccagcg gctcaaccta caagtttctc cagatgccgt taccaacagg cgtcagcagg
     1981 gagaggctac caataaaatt gccttcgtgt tattccttaa tcgcggagac gactccggcg
     2041 ccgacggtcc ggccgccttc gcggatagcg aagcgcagct tctcttccat ggcgatcgga
     2101 gcaatcagct cgacgtccat cgttatattg tctccaggca tcaccatctc cgtgccctcg
     2161 ggcaaggtca cgaccccagt cacatccgtt gtgcggaaat agaactgcgg ccggtaattg
     2221 gtgaaaaacg gcgtatgacg gccgccctcg tccttggtca gaatataggc ttctgccttg
     2281 aattgggtgt gcggcttcac cgaacccggc ttgcacaaga cctggccgcg ttcaacttct
     2341 tcacgcttgg tgccacgcaa aagcgcgccg atgttgtctc ctgcctgacc ctgatcgagc
     2401 agcttgcgga acatctcaac gccggtcaca gttgtcttgg tggttggccg cagaccgacg
     2461 atctcaacct cctcgccgac cttgaccgcg ccacgctcga cccggccggt cgcgacagtg
     2521 ccgcggccgg aaatcgagaa gacgtcctcg accggcatca agaatggctg atcgatggga
     2581 cgctcgggtt gcggaatgta ggaatcgacg ctctccatga gcttcaatac ggaatcgtgt
     2641 ccgatctccg ggctgcgccc ttcgagcgcg aataatgccg atcctcgggt gatgggaata
     2701 tcgtcgccgg gaaagtcgta tttcgacaaa agctcgcgga cctcgagctc gacgagttcg
     2761 agaagctcgg agtcgtcgac catgtcgacc ttgttgagat agacgactag ggccgggacg
     2821 ccgacctgcc ttgccagcag aatatgctcg cgcgtctgcg gcatcggccc atcggcggcc
     2881 gagacgacga gaatggctcc atccatctgc gccgcgccgg tgatcatatt tttcacatag
     2941 tcggcgtggc cgggacaatc gacatgggcg tagtgccgct tctgggtctg atattcgaca
     3001 tgcgctgttg agatcgtaat cccgcgcgcc ttttcctctg gcgccttgtc gatctggtca
     3061 taggcggtaa aggtcgcccc gccagtctca gccagaacct tggtaatcgc cgccgtcagc
     3121 gtcgtcttgc cgtggtcgac atgaccaatc gtcccaatgt tgcaatgcgg ctttgtgcgt
     3181 gcgaattttt ccttgcccat tgtctggctc ctctaactat tcttcattcg cgttaccata
     3241 agatcaggca tatttggcct ggatcttccc agcttcgcct gctgggacct gctcataatg
     3301 atcgaattgc atcgtgtagt ttgcgcggcc ctggctcatg gatcgcagtt gattcacata
     3361 gccaaacata ttggcgagag gaaccatggc gttaatcacc acggcattgc cgcgcatgtc
     3421 ttggccatgg acatgcccgc gcctgcccaa aaggtcgccc atgaccgagc ctgtgtaatc
     3481 ctccggcgtc gtgacctcga ctttcataat cggttcgagc aggacacagc cgcctttctg
     3541 cagcgcctcg cgaaatgcgg cgcgggcggc aatctcaaaa gcaagcaccg acgaatcgac
     3601 gtcgtggtac gcgccgtcga tcaatgtcgc cttaacgtcg acgaccggga aaccggcgag
     3661 gaccccggat cccatgacgc tttcgatacc cttttcgaca ccgggaatgt attcctttgg
     3721 aaccgcgcca ccgacgactt gcgactcgaa gcctgaacct atccctgctt cggttggctc
     3781 aaagacgatc ttgactcggg cgaattgacc ggaacctccg gtctgtttct tatgggtgtg
     3841 atcgatttcg acgcgccgtg tcattttctc gcgataggcg acttgcggtg cgccgatgtt
     3901 ggcatcaacc ttataggtgc gccgtaaaat atcgacctta atgtcaagat ggagctcgcc
     3961 catgcctttc aagatggttt gcccggactc ctgatccgtc gaaacccgga aagagggatc
     4021 ttccgcggcg agcttggcca aggccacgcc gagcttttcc tggtcggcct tggacttcgg
     4081 ttcgatagca atctcgataa ctgggtcggg aaattccatt cgctctagga cgaccggctt
     4141 gttgagatcg caaagcgtgt ctccggtcct ggtatccttc aagccagcaa gcgcgacgat
     4201 atcgcccgaa taggcttcct taatgtcctc acggttgttg gcgtgcatga gtagcatgcg
     4261 gccaatccgt tctttctttt ccttagtcga gttcaaaacg gtcgtgcccg actcgacatg
     4321 gccggaataa acgcgtgcaa aggtaatcgt gccgacaaag ggatcgtcca tgatcttgaa
     4381 agcaagcatg gagaaaggat cttcatcgcg cggcaggcgg agcacttccg cgccagtctc
     4441 catatcggta cccttgatcg cctcgcgatc gcgcggcgaa ggcagaaaat caacgacagc
     4501 gtcaagcaga ggctggacgc ctttgttctt gaatgccgag ccgcacagca ccggaataaa
     4561 ggcgatctca cggaccgcct tgcgcagaag ccgctttaaa atctccgtgt ctggttccgt
     4621 cccctcgaga taagcagcca tgacatcatc gtcgagctcg acagctgcct cgacgagcat
     4681 gtgtctgtac tcgtccgcct tggccttcaa atcttcgggg atttccgcgt ccgcataatt
     4741 ggcgccgagc gcctcatctt cccaaaccac ggccttcatc cgcacaaggt cgatgatgcc
     4801 cctgaagtct gattctgcgc caagcggcag ctggatgccg ataggccgag cgcccacctt
     4861 ggttttgaga tcctcgacgc agcggtagaa atcggctccg attttatcca ttttgttgac
     4921 gaagacgatg cgcggcacat tatatttgtc ggcttggcgc cagacggttt cggtctgcgg
     4981 ttctacgccc tgattgccat cgagaacgca gaccgccccg tcgagcacgc gaagcgagcg
     5041 ctcgacctcg attgtgaaat cgacgtggcc aggggtgtcg atgatattca ggcgcttacc
     5101 ccgccaaaag gttgtcgtcg cggcagaggt gatcgttatg ccgcgctcct gctcctgttc
     5161 catccaatcc atagtggcgg cgccgtcgtg gacttcgccg atcttatgcg atttgccaga
     5221 gtaataaaga atccgctcgg tcgtcgttgt cttgcccgcg tcgatatggg ccatgatacc
     5281 gaagttacgg tagtcttcga tcggatggct gcggggcatg acaaaattcc ttcgagaaaa
     5341 actggcttat cttgcgttaa actaccaacg ataatgcgag aaggcgcggt tggcttccgc
     5401 catgcggtgg gtgtcttccc gcttcttgac cgcattgccg cgattatttg cggcgtccat
     5461 caactccgct gacagacgat cgaccatcgt cttgtcgttg cggccgcgcg cggcactgat
     5521 gatccagcga atggcaagcg cttgccggcg ctccgagcga acctcgaccg gcacctgata
     5581 ggttgcgcca ccgacccggc gcgagcgaac ctcgattgaa ggagcgacat tatcaagcgc
     5641 ctgcttgaag atcgcaagcg gttccacgcg cgatttctgc tcaatcgaat cgaaagcgcc
     5701 atagacgatc gtctcggcaa tcgacttctt gccgtcgtgc atgatcgagt tcatgaattt
     5761 ggtgacgacg atatccccga acttcgcgtc cggaataatg tctcgttttt cggcgcgatg
     5821 gcggcgcgac atttggtggt ctccgtctaa acgaattaag ttctacaggg ttgccactaa
     5881 aggctgccag atccgcgatc agactatctg cctcgccatc ccaaaggcgt tgcgaatgtc
     5941 acttcggccg cttggcgcca tatttggacc ggcgctgctt gcggttcttg acgccctgag
     6001 tgtcgagcac gccgcgtaag atatgatagc gcacgcccgg cagatccttc acccgtccgc
     6061 cgcggatcat gaccacggaa tgctcttgca aattatggcc ctcgcccggg atataaccga
     6121 tgacctcgaa cccattggtc aggcgcacct tggcaacttt gcgcagcgcg gagttcggct
     6181 ttttgggcgt tgttgtgtag acgcgcgtgc agacgccccg cttctgcggg cacgccccga
     6241 gatggcgtgc cttttcgcga tagctctttt cctgccgcgg cttgcggatc aattgactga
     6301 tcgtcggcat aagtctctac tctgtcattc cttcaagcca aggccggggc ccagcggaac
     6361 caaattgtga ggcctccagc cgcacatgca ctggcgcgga cgcataggcc aaagcgccgc
     6421 gagcccggtg gagccttcgg cgcggcattg tggcagagga ccacggcatc aaaaaccgcg
     6481 ttctcgcctc ttgccgaccc aagcaaaatc ttggatcgtc gcgtattatc acttcgctgg
     6541 atgtgacagc gtttaggttt ggtatctccg agacaaggcg ccacggaagc gaaaacctac
     6601 tgcctgtccg aaaaacggcc ggaacttagt cttgcccttc cggcacgtca acccttattc
     6661 gtcggaaaat ctatagcagc ggcggaaaat tcttgcgggg atcattctgc gcagatgtac
     6721 ggctgccaag gcgaggatgc aagacttttg cgatcgtacg aacttaaggt cgtcttccga
     6781 acttacgcga tcggcatcca gctgactggc gcgaactcca aacgaaagaa tgctcggatg
     6841 gcgacgaaaa agcttcacac cgcgttggct tccttcattt ttcttgcggc cattgtatca
     6901 cccgcggctc aggacatatc cctcgagacg caactcctcg acgctatgag taagctttcg
     6961 ggcgttcatc ccggattccg cgccaatcat gccacgggaa tcgtcgtggc gggaagcttc
     7021 gaggccacgc cacacgccgc ccaattgagc aaggcggttt tgttttcagg ccttaaaatc
     7081 cccgcgacgg cacgtttctc ggatgtcccg aatatagaag atggctctgc tttcgcaaac
     7141 ccatggcatg gcgatcgaat cccatcttat ccgaaaccga catcgtcctc aactcgctca
     7201 aattccttcc ggtatccggc gctgcagatt tccgggatat gctggttgca aaggctgaga
     7261 gcccaccaaa ggctccgaaa ccgacaacgc tcgagcaatt catcgcgacc catccgactg
     7321 tgccgcttgc gctagcgatg gcacagaacc cccgacagtt tcgccgacga agactttctg
     7381 gcatcaattg catcgaaaaa cgcccggtac gctacattgc caagccagag aaattggtgc
     7441 acatcgagcc gacggaagca gcgaagatgg cgcccgacct gtttgatgga gctggcggcg
     7501 cgtctatgcc cgaggccccg taaagtttcg tctaacggcc cagcttgcac caccgggtga
     7561 cgcaaccacg gatccttcgc ggccgtggcc ggaaagcaac gaaatcgtcg agcttgcgtg
     7621 ctgacgatcg ccaagccggt gtccgacagc tccgacacgc aaagaaagcc cctgttcctg
     7681 cctggacagt tcacggaagg gatcgaatcc tcgggcgccc cgatgatcga tcgcgcaacg
     7741 gggccatgct atttctttct ccccgcagag ggcagcctga cgtctcccag gacgtcccaa
     7801 cagcgtcggt cgacctcctg gctggcacgt gggatcggtg actatcgaga tgagacggcc
     7861 aggcgaagtt gacggtcggg attggtccac aaaccaaccc ttcgcaaaaa ggactcggtc
     7921 ctagctcttg atctctcgac gcccaagata attagccagg ttaacgccta cctccggagg
     7981 tgcttgccgt gcctgggatt catggcgcag ccgcgcccga aattcactta gcctatgcgc
     8041 cgcggcgaaa atgtctgttg aaagcagccc agtgaaatag ccaaactcga gactgaggat
     8101 caggaggcca acgctggcca gggaatcaag gacggttttt tcagccattt ggcaattcat
     8161 ccattgcaaa gcggccgcaa ccgcgcagac gggaattaga accaaaacct tgaagaatcg
     8221 cccaattatc gcgcctaaaa gggcacctcc agcaatcaga agttcgatca tatggcctcg
     8281 cgtattggcc ccgcacggct taccttgact cggcaatctt gcgtgacgct ttggaatgtt
     8341 gcgtaacgct tacaattgga gaactaatat catatcaaaa ttgccataag gtaaagtcct
     8401 atttttggcc acattcgaaa ttaagccaag ccagcgcttt tggaaggccc gccaaggcga
     8461 cgatcccacc cattgcggtg ccgtcaattg tggccttgac ctcgacctcc ttggcctcgc
     8521 catggccaag ggcagaaagc tttgccgggt ccattagcaa acggatgcct gctcctgttg
     8581 gtatcatgct ggcggcaaat tcataacgct gttccggcgt tcgcaaacta actcggggca
     8641 gcgcgtgggg agaaaaaggt tccacaacaa tcaagacagt ttcgatccct tgcgccccgc
     8701 atctcagctt gagaccagcc aagcgtggat cggagcggtc gaaatccgct gtatgtagaa
     8761 cggccgcgca atctttcaag gcgttcgtct ttgcgcacgg aactcgccgc cagccgccct
     8821 cagccccggc accaatatct gcgtagcctg cggatgtgtc gtctcccacc ggcggcgcgg
     8881 tgaaactcgg atggccgccc tcgcccgcgg cgcacggaag ccatggacca cacgcaaatg
     8941 cagcttgaaa ggcaagacag aagccgagcc tgcggcaaat catcggcacg aacgaccgcg
     9001 ggcgttcagt tgcgttgcac aatttctttg tgggggcatg ctaccgtgtc tggacatggc
     9061 cgcagtctcc tttttggtga tcatacagt
//
LOCUS       DATMPU010000333         4349 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_4583226,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000333 DATMPU010000000
VERSION     DATMPU010000333.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 4349)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4349)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4349
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4583226"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2909)
                     /gene="putA"
                     /locus_tag="VNY06_07605"
     CDS             complement(<1..2909)
                     /gene="putA"
                     /locus_tag="VNY06_07605"
                     /EC_number="1.2.1.88"
                     /EC_number="1.5.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012590677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0003842 - 1-pyrroline-5-carboxylate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0004657 - proline dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0010133 - proline catabolic process to
                     glutamate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional proline dehydrogenase/L-glutamate
                     gamma-semialdehyde dehydrogenase PutA"
                     /protein_id="HWX14700.1"
                     /translation="MSPDLPHRPFSAPYGVDDEVLAAELLGGAALSKESEARIDQRAL
                     GLIDAVRTKSNRIGGIEDLLREYSLSSEEGVALMVLAESLLRVPDDLTADRLIKDKLA
                     SSDWSHQAGDSDSLLVSAAAWALGLSARVIGKGKTPEGVVATLARRIGTSALRAAARQ
                     AMQLMGSHFIFGETIEDALARARSREGRNYRYSYDMLGEGARTKDDAARYFESYANAI
                     EAIGTSGGSSRLPMRPGISVKLSALHPRYEPLSAPRVLAELTPRAAELARMAKRHDLN
                     FTIDAEEAERLELSLDIIDQLLADPALSGWDGFGLAVQAYQKRAEAVIVYIAAQARRY
                     GRRLMVRLVKGAYWDTEIKRAQEQGLANYPVFSRKAMTDLNYLACAKKLLDLRDIIYP
                     QFATHNALTIATIIELAGNNECCEFQRLHGMGEDIFAALRAEPFPAPACRIYAPVGSH
                     ENLLAYLVRRLLENGANSSFVARLGDPHVERSELLIRPEIIIGDKEHARAQDLRLPAD
                     IQMPSRKTANGITFGDRKALAAITRAIAQSTRQREAAPVICGIIRDGTVRDVVSPIDG
                     TRIGAVREASPSLLTEALGEARAGFCHWSNVPAGVRAACLNRASGLLETEAPAFLGLL
                     QTEAGKTLEDAIGEWREAIDFCRYYAQEARRLFASATIMPGPTGEDNRLSCHGRGVFV
                     CISPWNFPLSIFLGQIAAALAAGNSVLAKPAVQTPLIAAATVELLHRAGVPGPALQLL
                     PGGGELGEMLVSLPNIAGVAFTGSTGTATKINRVLAAKDGPIPVLIAETGGINTMIAD
                     ATALPEQVCDDVLASAFRSAGQRCSALRLLCVQEDVANKLITMICGAARELLVGDPRD
                     TQTHIGPVIDGAAAASLNAHVAAMRSSTILRFAGTIPRSTQQGGFYVAPHVFEIAHAN
                     DLKREIFGPILHVVRYQAAELDPLLDAIESAGYGLTLGVHSRIDATIDRILGRRLA"
     gene            complement(3013..3825)
                     /gene="dapB"
                     /locus_tag="VNY06_07610"
     CDS             complement(3013..3825)
                     /gene="dapB"
                     /locus_tag="VNY06_07610"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012383148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HWX14701.1"
                     /translation="MSDMRLVVAGAAGRMGRVLVEIIRQTPGAHLAAAIEQPDSIAIG
                     QDAGLLAGCGQMGVAISGDALTAVAGADGILDFTSPQSTVELAALAAQARIVHVIGTT
                     GLKASDLTKLDAAARHAVIVRSGNMSLGVNLIAALVAMAAHTLGPEYDIEIVEMHHRM
                     KVDAPSGTAFLLGEAAAKGRGVELEQHSVRSRDGHTGVRRAGDIGFASLRGGTVIGDH
                     KVIFAGPGERIEFAHVAEDRSIFARGAVRAALWGRNQKPGLYSMTNVLGLSG"
     gene            complement(3938..4152)
                     /locus_tag="VNY06_07615"
                     /pseudo
     CDS             complement(3938..4152)
                     /locus_tag="VNY06_07615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012253131.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
BASE COUNT          879 a         1308 c         1329 g          833 t
ORIGIN      
        1 gcaagcctcc ggcccaaaat acgatcaatc gtggcatcga tgcgcgaatg gactcccaag
       61 gtcagcccat aaccggcaga ttcgattgca tcaagcaagg gatcaagctc ggccgcctgg
      121 taacggacga catgaaggat agggccaaaa atctcgcgct tgaggtcatt tgcgtgagca
      181 atctcaaaaa catgtggcgc aacgtaaaat ccgccttgtt gcgtgcttcg tggtatcgtg
      241 ccggcgaagc gaaggattgt tgagctgcgc atcgctgcga catgcgcatt aaggctagcc
      301 gccgcggcgc catcaatgac cggcccaatg tgggtctgtg tgtcgcgcgg atcaccaacc
      361 aaaagctcgc gggccgcacc gcagatcatc gtaatcaatt tgttcgcaac gtcctcctgg
      421 acacaaagga gccgcagcgc ggaacagcgt tgtcctgccg agcgaaatgc cgaggcaagg
      481 acgtcatcgc agacctgctc aggcagcgct gtcgcatcgg cgatcatggt gttgatcccg
      541 ccagtttcgg cgatcagcac cggaattggc ccatcttttg ctgccaaaac gcgattgatt
      601 ttcgtggcgg ttccggttga gccagtgaag gctacccccg cgatattggg cagggagacg
      661 agcatttcgc caagctcgcc tcctcccggc aagagttgta atgccgggcc cggcacacct
      721 gcgcggtgga gtagctcgac ggtggcggct gcgatcagcg gcgtctggac tgcgggcttg
      781 gcaaggacgc tgtttccagc ggcaagcgct gctgcgatct ggccgagaaa aatcgataat
      841 ggaaagttcc aagggctgat gcagacaaaa acgcctcggc catggcaact caaccgattg
      901 tcctcgcctg tcggtccagg cataattgtg gcgctagcga aaagccggcg cgcctcttgc
      961 gcatagtagc ggcagaagtc gatcgcctcc cgccattcac cgattgcgtc ctccagcgtc
     1021 tttcccgcct cagtttgcag taggccgaga aaggcgggcg cttcggtttc aagtaagccc
     1081 gaagcacgat taagacaggc cgcccggacg ccggccggaa cgttagacca atggcaaaaa
     1141 cccgcacgcg cctcgcctag ggcttcggtc agaaggctgg gcgaagcctc gcggacagcg
     1201 ccaatccttg ttccatcaat tggcgataca acgtcgcgca cggttccatc gcggattatg
     1261 ccgcagatga ctggcgcggc ctcgcgttgg cgcgtggatt gggcgatcgc cctcgtgata
     1321 gcggcaagcg ccttgcgatc gccgaacgtg attccgtttg ccgtcttccg cgacggcatt
     1381 tgaatatcgg cgggtagccg caggtcctgc gcacgggcat gctctttgtc accaatgatg
     1441 atctctggcc ggatgaggag ctcggagcgt tcaacatgcg ggtcacctaa cctcgcaacg
     1501 aaggacgagt tggcgccgtt ctcaagcagc cgccgcacaa gataggcgag cagattttca
     1561 tgactgccaa ccggcgcata gatccggcac gccggtgccg gaaagggctc cgcccgtaag
     1621 gcagcaaaga tgtcctcgcc catgccatgg aggcgctgga attcgcaaca ctcattgttt
     1681 ccggcaagtt cgataattgt tgcaatggtc aaagcgttat gggtcgcaaa ttgcggatag
     1741 ataatgtcgc gcaggtcgag gagttttttg gcgcatgcaa gataattaag atcggtcatt
     1801 gctttgcgcg aaaataccgg gtagttggcg agaccctgct cttgcgccct tttgatctcc
     1861 gtgtcccaat aggcgccctt tacgaggcgc accattaagc ggcggccata gcggcgagcc
     1921 tgtgccgcta tataaacgat tactgcctca gcgcgctttt gataggcttg cacggcgagc
     1981 ccgaaaccgt cccatccgga aagcgcggga tcggcgagaa gctgatcgat tatatcaagc
     2041 gacagctcca gccgttcggc ttcctcggca tcgatagtaa agtttaaatc gtggcgcttg
     2101 gccatgcgcg cgagttcggc agctcgcggc gtaagttccg caagcacgcg cggagccgac
     2161 aagggctcgt agcgcggatg caacgctgag agcttgaccg aaatgcccgg cctcattggc
     2221 aagcggctag agcctccgga agtgccgatt gcttcgatgg cattcgcata tgattcaaaa
     2281 tagcgcgcgg cgtcgtcttt cgtccgcgcg ccttcgccca gcatatcata ggaatagcgg
     2341 taattccgtc cctctctcga tcgcgctcga gctaacgcgt cctcaatcgt ttcgccgaag
     2401 atgaaatgcg atcccataag ctgcatggcc tggcgtgcgg cggcgcgcag cgcgctggtg
     2461 ccgatgcggc gggcgagcgt cgcaactaca ccctcgggcg tttttccttt gccgatgaca
     2521 cgcgcgctga ggcccagggc ccaagccgcg gccgaaacga ggagagaatc cgaatccccc
     2581 gcctgatggc tccagtcaga agaggcgagc ttgtctttga tgagccggtc tgcggtgagg
     2641 tcgtcaggca cccgcagcag cgactccgcg agaaccatca aagcgacgcc ctcttccgag
     2701 gacaaggaat attcgcgcaa caggtcttcg atcccgccga tgcggtttga tttggttcgg
     2761 accgcatcga tcagccccag ggcgcgctgg tcgattcgcg cctcggactc tttggagagc
     2821 gcagcgcccc caagaagctc ggcggcaaga acttcgtcgt ctaccccgta tggagcagaa
     2881 aaaggacgat gaggcaggtc cgggctcatg cacaaaatgt tccacaaccg ctgctgacgg
     2941 tgcgcaatat atggggtgac cgtggtgtgc tttcaccgcc agtatgcttt gtgggctgga
     3001 aaacgagaga attcagccgg aaagtccaag cacattggtc atcgagtaaa ggcctggctt
     3061 ttggttcctg ccccagagcg cagcccgcac cgcaccgcgc gcgaagatgc tgcggtcctc
     3121 cgcgacatga gcgaattcga tccgctcgcc tggccccgca aaaatcactt tgtgatctcc
     3181 gatgacggtc ccgccacgca gcgatgcaaa gccgatatcg ccggcgcggc gcacgccggt
     3241 gtggccgtcg cgcgagcgga ccgaatgctg ttcgagttcg accccgcggc ctttggctgc
     3301 ggcctcaccc aggagaaacg ccgtgccgga cggagcgtcg actttcatgc gatggtgcat
     3361 ctcgacgatt tcgatatcat attccggccc aagcgtatgc gcggccatcg cgacgagagc
     3421 ggcgatcaga ttgacgccta ggctcatgtt gcccgagcgc acgatgacgg catggcgcgc
     3481 ggcggcatcg agctttgtta gatcgctggc tttcaagccg gtcgtcccga ttacatggac
     3541 gatgcgcgcc tgtgctgcga gcgccgcaag ttcgaccgtc gattgcggcg aagtgaaatc
     3601 gaggattccg tcggcgcccg caacggctgt aagggcatcg ccgcttattg ccacacccat
     3661 ttggccgcag ccggcgagga gccccgcatc ctggccgatg gcaatggaat cgggctgctc
     3721 gatcgccgca gcgagatgcg cgccgggcgt ctggcggata atttcaacaa ggacgcggcc
     3781 catgcgtcca gccgcccccg caacgacaag gcgcatatcg ctcatgacgg atgtccccga
     3841 tttatcctag ccttgtctat aatccctgga ggccgcaagg aaaactccag attagcggaa
     3901 gaacgcacca tgatcgcgcg cgccgggccg gaggtacgcc cggcgtcatg gttctttttt
     3961 ggtcgccagc ttccgctccc gccggccgta tacccgggca caacgcgcgc gcatggcgca
     4021 tacctcatgg gcgcaggcgc gcgcttgcgc cgcatggcgg gcgacgaggt cagccactcc
     4081 ataggcataa ccggcctcag cgaggtcctt atcgttgtag gacgtcaaaa cggttacacc
     4141 gaggagagga agtcttcgag cgcccgcggc aaatttcgcg gcctgggcgc tagccaggct
     4201 cgcctcggct tcccgtcacc tctcgaaatc gagcgcaatg atgagctttt ttcgggcggc
     4261 ggagtgtcgt gcggggcggt ggcagggcgg ttcatcccaa atggagggaa ttaccgctgc
     4321 cagatgtctt gtgcgcgctg tccacagcc
//
LOCUS       DATMPU010000334         3439 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_2131644,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000334 DATMPU010000000
VERSION     DATMPU010000334.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 3439)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3439)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3439
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2131644"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..932)
                     /locus_tag="VNY06_07620"
     CDS             complement(<1..932)
                     /locus_tag="VNY06_07620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018263027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX14702.1"
                     /translation="MQTRVAERGRAPANAAKEPKPNLKFPVRLASLERAAPPVAVIPS
                     RIPYNAGALTSRDHKKPARIVARERSRLQTLVAERGRASAKHAPSPKPRVRLASLEGT
                     APPAQAIPSRISSNTGALTSLVNFETAPFPYHGTVPDSGQPFLNAETEGHWGHVNFRG
                     NVLWESPTFSDDRVLLHIPPGFDPKRPAVMVVFFHGHGANLAQDVYDRQQVPAQITAA
                     GVNAVLVAPQFAVDAADSSAGKFWEPNGFRRFLDEAAVKLARMYGDPHSAFTFARMPI
                     VIVAYSGGFGPTLSVLDRGGVRSRVRGLVLLDAL"
     gene            complement(1529..2779)
                     /locus_tag="VNY06_07625"
     CDS             complement(1529..2779)
                     /locus_tag="VNY06_07625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX14703.1"
                     /translation="MAVLALNTAPARSSEGATANDTARFLAGLPPSSNSPLASYTKDP
                     VWQQHARYFDSIFAREENAKLSKVREFSNKYLTDKHDTMLYMFSGPDFLYATSFFPNA
                     STYVLAGLEPVGEIADLTTLSPWAINGELRNLELSMDSLFNFSFFITQNMKTQLREGP
                     VNGTLPILYVFLARTGKTIHELNLVSLDEHGNFQIAAELAATNSTNKPVTSPPRSAAT
                     GVKIIFSDGAGPKQTLYYFTTNLADGSFERSGFSAFLAKLGPADSLIKSASYLLHKGH
                     FASVQKLLLNSSATILQDDSGIPLAYFEARKWRFQAFGHYAGPISIFTNFYQPQMAEL
                     FKGARPIEFGIGYRWRKNESNLLLAQKESSPINDAELTPPSRAEGNAAGTAAPSPKKT
                     RKRVETEGTRSAGCRIARVFPFCW"
     gene            complement(3176..>3439)
                     /locus_tag="VNY06_07630"
     CDS             complement(3176..>3439)
                     /locus_tag="VNY06_07630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX14704.1"
                     /translation="IKMKKAGDACGARVNVDWLSYPILDITETPEQVDVVLINHPDLP
                     LTGAGEPSIRPVAAAIANAIFDATGVRIRRVPFSPNRVKQALS"
BASE COUNT          700 a          982 c         1050 g          707 t
ORIGIN      
        1 tacagcgcat cgaggagaac caggccgcgc acccgcgatc tgacgccgcc ccggtcgagg
       61 accgacaggg tcggtccaaa gcccccgctg taggcgacga ttacgatcgg catcctcgcg
      121 aaggtgaatg cactgtgcgg atcgccgtac attcgcgcaa gcttgacggc cgcttcatca
      181 aggaagcgcc tgaagccgtt cggctcccag aatttcccgg cgctcgaatc ggcggcgtcg
      241 acggcaaatt gcggcgccac caacaccgca tttactccgg cggccgtgat ctgcgccggc
      301 acctgctggc ggtcgtacac gtcttgagcg agattggcgc cgtggccgtg aaaaaacacg
      361 accatgaccg ccgggcgctt gggatcgaag cctggcggaa tgtgcaagag cacgcggtca
      421 tcactgaaag tcggagactc ccaaagaaca ttgccgcgga aattgacgtg gccccaatgg
      481 ccctcagtct ccgcattcaa aaacggttgc cccgaatctg gcacggtgcc gtgatatggg
      541 aagggcgccg tctcgaagtt gacgagactt gtcagtgcgc cggtgttgga cgagatgcgc
      601 gaaggaatag cctgcgccgg cggtgccgta ccttcgagcg acgccagccg gactcgcggc
      661 ttcgggcttg gcgcgtgctt cgctgatgcg cgaccgcgct cggcgaccag tgtctgcaag
      721 cggctccgtt cgcgagccac gatccgggca ggtttcttgt ggtcgcgact tgtcagtgcg
      781 ccggcgttgt atgggatgcg cgacggaatg accgccacgg gcggtgcggc gcgttcgagc
      841 gacgcaagcc ggaccggaaa ctttaaattc ggttttggct cttttgccgc gttcgccggt
      901 gcgcggccgc gctctgcgac gcgcgtctgc aagctactcc attcgcgcgc cacgatcctg
      961 gcgggggtct cgcggccgcg acctgtgcct atcacatgcg gattggctga cggtgcatag
     1021 gctgagctgc tgaacggatc cagttttcca attttggaac tggcttttcc ccgacaccga
     1081 tttaaacgct ggcaatggga aatatctggg tgaattgatg cttcggccgg cgaaaccaaa
     1141 acagccgacc ggagctcgct caaggccggc cagcaccagc cgactgagcc aacgaacaac
     1201 aggatggcgg agaaagcggg aggaagtttt cgcttgagcc cgtccgggca ccgcaccgtt
     1261 tttttacttg gctgaaatcg aacgaggccg gtctcgaagt ggctgcgcga cggagccgca
     1321 cccaggtcga tattttcaag ccgccgccga aatgttgtgt ccgctgcaaa atagtgcgca
     1381 ggtgtaggcg gcaccacgat tatggccggc ctgcaaacag cgcgcaacgt tggtcccccg
     1441 accgtatctg acaccgtttc cccacccgag caagtcagcc tcgaccgcga ctatgtcttc
     1501 attttgaggc acgcctgcta atttgctctt accaacagaa cgggaagact ctcgcgattc
     1561 ggcagcctgc cgaccgcgtc ccttcggttt ccacgcgctt tcgcgtcttt tttggcgatg
     1621 gggcggctgt tcctgcagcg ttcccctccg ctcgtgaagg cggcgttaac tctgcatcgt
     1681 taatcggcga tgactccttt tgtgcaagga gaaggttcga ctcgttctta cgccatcggt
     1741 atccgattcc gaattcgatc ggacgcgccc ctttaaacaa ctccgccatt tggggctggt
     1801 agaagtttgt aaagattgaa atgggcccag cataatgtcc aaacgcctga aatcgccatt
     1861 tccttgcttc gaaataggca agcgggatac cgctatcgtc ctgaaggata gttgcgctgc
     1921 tattgagcaa caacttctgc acgctggcga agtgtccttt gtgcaagaga tatgacgcgc
     1981 tcttgataag gctgtcggca ggaccaagct tcgctaggaa ggccgaaaag ccgctccgct
     2041 caaatgaacc gtccgcgagg ttggtggtga agtagtaaag cgtctgcttt ggtccggcgc
     2101 cgtccgagaa gatgatcttc acgcccgtag cggcgctgcg aggtgggctt gtaacaggtt
     2161 tgttggtgct gttggttgcg gcaagctcag ccgcaatctg aaagtttcca tgttcgtcca
     2221 gactcacaag attaagttcg tggatggtct tgccggttcg cgccagaaaa acataaagga
     2281 tcggcaacgt gccgttgacc ggcccctcac gaagctgggt cttcatgttt tgggtgatga
     2341 agaaactgaa gttgaacagc gaatccatcg agagctcaag atttcgcagt tccccattga
     2401 tggcccaagg gctgagggtc gtcaggtccg cgatctcgcc caccggctcg agaccggcca
     2461 aaacgtacgt cgaggcatta ggaaagaacg aggttgcata gaggaagtcc ggcccgctga
     2521 acatgtaaag catcgtatcg tgtttatcgg tcagatattt gtttgaaaat tcgcggactt
     2581 tggaaagttt ggcgttctct tcgcgcgcga aaatcgaatc aaaatagcgg gcatgctgct
     2641 gccagacggg atctttggtg tatgacgcaa gcggagagtt cgacgacggc ggcaatccgg
     2701 ccaggaaccg cgcggtgtcg ttggccgtgg ccccctcaga gcttcgtgcg ggcgcggtat
     2761 tgagcgccag cacggccatc aggacagccg caactattct aggcatggct tccgaacttt
     2821 ttcaggcggc cgtttgcgaa acatcccaag aagagacatc cgtagcaggc aggacacgca
     2881 gtagacgaga agtccggtgc aaaagccccg cccagtgagc cggggcttgc tgaaaggcca
     2941 ggcaatcgat gacggccgtt ccgcgcgcgc ctgctgaagc gtttcccgac ctattggcag
     3001 ccgagcaggt gtcacggtca taagctattg ggtttgcgcg cagctggaag ccactcgcgg
     3061 cttacacgga cccctaaggt gagccggcaa gccctgccag tgtttccggg ctctgaaaag
     3121 aaataaagac ggcattgcga gctggctttt gtttagatcg cgtttcccgc ggcagtcagg
     3181 acaacgcctg tttaacccga tttggcgaga aaggcacgcg gcgtatgcgc acgccagtag
     3241 cgtcgaagat cgcgttggcg atcgccgcgg ccactgggcg gatcgatggc tcgccggcgc
     3301 cggttagcgg caagtccggg tggttgatta gcaccacgtc aacctgctcg ggcgtctcgg
     3361 tgatgtcgag gatcggatag ctcagccaat ccacgttcac acgagcaccg catgcatcgc
     3421 cggccttttt cattttgat
//
LOCUS       DATMPU010000335         6370 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_3357585,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000335 DATMPU010000000
VERSION     DATMPU010000335.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 6370)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6370)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6370
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3357585"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..294
                     /locus_tag="VNY06_07635"
     CDS             <1..294
                     /locus_tag="VNY06_07635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX14705.1"
                     /translation="QYSRRSFTAHYIPESHRFLQFQSEINALKLRSINGMNVHHPKDL
                     GSFSNRVILAIETTVPKSFQFAKRLGLKVVTSLSAGRAKTAERSCAQPDHDSS"
     gene            complement(347..1612)
                     /gene="odhB"
                     /locus_tag="VNY06_07640"
     CDS             complement(347..1612)
                     /gene="odhB"
                     /locus_tag="VNY06_07640"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018388039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004149 - dihydrolipoyllysine-residue
                     succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HWX14706.1"
                     /translation="MATEIRVPSLGESVSEATVGRWFKAPGEPVKADEPVVELETDKV
                     TLEVNAPALGVLSEILAREGETVAVGALLGHVGEGAAAAPPKAETKPVQPPATPSGQP
                     AFASDAPLTAATAEAAAPRSLPASPAAAKIAADHGLDPAMVAGSGKRGQVLKSDVLDF
                     IAKPPAVAEIPAPPPAPAAAPSLALGSDETREERVPMTRLRQTIARRLKDAQNTAAML
                     TTFNEADMSAVMSLRARYKDAFEQKHGIKLGFIGLFVKACVGALKAVPAVNAEIDGVD
                     MIYKNYYHLGVAVGTDKGLVVPVVRDCDRLSLGEIENSIAGLGKRARSGALKIEEMQG
                     GTFTITNGGVYGSLMSTPILNPPQSGILGMHKIQERPVAVNGKVEIRPMMYLALTYDH
                     RAVDGKEAVTFLVRVKEALEDPARLMLDI"
     gene            complement(1754..2200)
                     /locus_tag="VNY06_07645"
     CDS             complement(1754..2200)
                     /locus_tag="VNY06_07645"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HWX14707.1"
                     /translation="MSHIKSVTPGTAGTIKLGSTTVRVLEDGRLTDNRIGAIVCTVPA
                     QTKPLPQHLHRMHDETFLVIQGIIRFAVNEEGHDVKTGEYVVVPTGAAHTFSNPFDEP
                     AAFVTTFTPAYYVNYFRELGKVISAGKEPSTDEMITIRGHYATEPA"
     gene            complement(2251..5259)
                     /locus_tag="VNY06_07650"
     CDS             complement(2251..5259)
                     /locus_tag="VNY06_07650"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020177216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HWX14708.1"
                     /translation="MARQDTNGNANGAGGGPGGRSAPNLGFARTSFLDGANAAYIEQL
                     ADNYERDPASLDPSWREFFAQLKDDRFAIKRRARGPRWTQQSLPPTAADELISALDGN
                     WREAQTAIGAKLEAKAKEGGLFSSEELLRATRDSVRALMMIRAYRMRGHLHANLDPLG
                     IEPPKDREELHPAAYGFTEKDYNRKIFLDGVLGLKFATIPEMLKILRRTYCGTIGYEF
                     MHISDPAEKGWLQQRIEGPGKEIIFTNEGKRAILNKLVEAEGFEQFIDIKYTGTKRFG
                     LDGAEAMIPALEQIIKRGGALGVQDIVLGMAHRGRLNVLSHVMGKPHRAIFNEFKGGS
                     LTPDDVEGSGDVKYHLGASADRDFDDNRVHLSLTANPSHLEIVDPVVLGKVRAKQDQL
                     YDVVERKKVLPLLIHGDAAFAGQGVIAECFGLSGLKGYRTGGSIHFIINNQIGFTTAP
                     RHSRSSPYPSDSAKLVEAPIIHVNGDDPEAVVYAAKVTTEFRQRFHKPVVIDMFCYRR
                     FGHNESDEPAFTQPVMYKKIRSHKTTLQIYTEKLVAEGVITEGEVIRLQANWRHHLET
                     AFEAGQSYKPNKADWLDGRWAGLRPAAAGIEEDRRGRTGVDRARLIEMGLQLTAVPEG
                     FRLHRTIGRLLENRRKMIETGVGIDWAMAEALAFASLVDEGYPVRLSGQDSERGTFSH
                     RHSVLIDQETEERYIPLNNIRDKQARFEVINSLLSEVAVLGFEYGYSLAEPNALVLWE
                     AQFGDFANGAQVVFDQFISSGERKWLRMSALVCLLPHGYEGQGPEHSSARLERYLQLC
                     AEDNMQVANCTTPANYFHILRRQLQRDIRKPLILMTPKSLLRHKRAVSKLEDLVIGKS
                     FHRILLDDAETARGGPITLAEDAEIRRVVLCSGKVYYDLYDEREKRGANDIYLLRVEQ
                     LYPFPLKALVAILSRFKQAEIFWCQEEPKNMGAWTFVGPYLEWVLGQAGCKTKRPVYA
                     GRPAAAATATGLMSKHLAELKAVMGEVYAR"
     gene            complement(5532..>6370)
                     /gene="sucD"
                     /locus_tag="VNY06_07655"
     CDS             complement(5532..>6370)
                     /gene="sucD"
                     /locus_tag="VNY06_07655"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018045794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HWX14709.1"
                     /translation="TGKNGTYHSEQAIFYGTKLVGGVSPGKGGATHLGLPVYSTVAAA
                     RSATGADASVIYVPPSGAADAICEAIAAEMPLIVCITEGIPVADMVKVKRALCGTKSR
                     LIGPNSPGVVTAGQCKIGIMPGDIFKPGSVGIVSRSGTLTYEAVFQTTIEGLGQTTAV
                     GIGGDPVKGTEFIEILELFLADDLTKSIVMIGEIGGSAEEEAAQFLADEAKRGRKKPV
                     VGFIAGRTAPPGRRMGHAGAIISGGKGGAEDKIAAMQAAGVYVSPSPARLGKTLADLL
                     RG"
BASE COUNT         1309 a         1852 c         1797 g         1412 t
ORIGIN      
        1 caatattcga gacggtcttt cacggcgcat tacatcccag aatcacatag gtttttacag
       61 ttccaaagcg agatcaacgc gttgaagctc agatccatca atggcatgaa tgttcatcat
      121 ccgaaggatc tgggttcatt cagcaaccga gtcattctgg cgatagagac gactgttcca
      181 aagtcgttcc aatttgcaaa gcggcttgga ctcaaggtgg tcaccagttt gtcggccggc
      241 cgagccaaga ctgcggagcg atcttgtgcg caaccggacc acgactcgag ctgactcatt
      301 gtgggcttgc gacaagaaca agttcgtagc gttgcacggg cttccatcaa atatccagca
      361 tcaaccgcgc cgggtcctcc agcgcttcct tgacgcggac caaaaaagtc accgcctcct
      421 tgccatcgac ggcgcggtga tcataggtaa gcgcaaggta catcatcggc cggatttcaa
      481 ctttgccatt tacagcgact ggccgctcct gaatcttgtg catgcccaag atacccgact
      541 gaggagggtt caaaatcggc gtcgacatca gcgacccata gacgccgcca ttcgtgatgg
      601 tgaatgtgcc accctgcatc tcttcaatct ttagcgcgcc cgatcgggcg cgcttaccaa
      661 gaccggcgat ggaattttcg atttcgccaa ggctcaagcg atcgcaatcg cgcacaaccg
      721 gcacgaccaa gcccttgtcc gtgccgacgg cgaccccgag atgatagtaa tttttgtaga
      781 tcatatcgac gccgtcgatc tctgcattga ccgcgggaac cgccttcagt gcgccgacac
      841 aggcttttac gaaaagtcct atgaagccga gttttatgcc gtgcttttgc tcgaaggcgt
      901 ccttgtagcg tgcgcgcaag ctcatgaccg cgctcatgtc ggcctcgttg aaggtcgtca
      961 gcatggcggc cgtattttgc gcgtccttga gccgccgcgc gattgtttgc cgcagccttg
     1021 tcatcggaac acgctcttcc cgggtttcgt cggagccgag ggccaaagac ggcgccgcag
     1081 ccggggcggg cggcggggca gggatctcag ccacggccgg gggttttgca ataaaatcga
     1141 gaacatcgct cttgagcacc tggccgcgct tgcccgatcc ggcaaccatt gccggatcga
     1201 ggccgtgatc ggcggcgatc ttggcggctg ccggcgaggc gggcaaggag cgcggagcgg
     1261 ccgcttcggc ggttgcagcg gttagcggcg catcggatgc aaaggcaggc tgaccggaag
     1321 gtgtggcggg tggctgaacg ggcttggttt cggccttggg aggcgcagcg gccgcgccct
     1381 ctccaacatg gccgaggaga gctcctacgg ctaccgtctc gccctccctg gcaagaattt
     1441 cagagagaac acctaatgct ggcgcattca cttcaagtgt tactttatct gtttcaagct
     1501 cgacgaccgg ctcatccgcc ttgacgggct caccgggtgc tttgaaccag cgcccaactg
     1561 ttgcttcgct gaccgattcg cctaaagacg gcacgcgaat ttcagttgcc atttgttcta
     1621 cccaaagtca ctcagtcaac tctctgtata aatgaacctg gatcacccgg tagtcgttgt
     1681 gaccccggct ctggatcgga aaacagggtt catcttttgc ttttgtgagt tcgtctcatc
     1741 acgtccgggg tcgctatgcc ggctcagttg cgtagtggcc cctgatcgtg atcatttcgt
     1801 ccgtcgaggg ttccttgccg gcggagatca ccttgccaag ctctctaaag tagttcacgt
     1861 aataagcggg cgtgaacgtg gtgacgaaag ctgcgggctc atcgaatggg ttggaaaacg
     1921 tgtgagcagc gccggttggg accacgacgt actcgcccgt cttcacgtca tgcccctcct
     1981 cgttcaccgc gaatcggatg atgccctgaa taaccaaaaa tgtctcgtcg tgcatgcggt
     2041 gcagatgttg cggcaaaggt tttgtttgag ctggcacagt acacacgata gccccgatac
     2101 ggttgtcggt gaggcgtccg tcttccaaaa cacgcacagt tgttgaaccc aacttgatcg
     2161 ttccggccgt tccaggcgta acacttttaa tgtgcgacat gcgatcttgc tccctttgtg
     2221 tggttataat taaataggca gcttaacatc tcatcgcgcg taaacctctc ccataaccgc
     2281 cttgagttcg gccaagtgct tagacataag ccccgtcgcg gtcgcggccg cggcgggcct
     2341 tccagcatag actggccgct tggttttgca gccagcctgg ccaagcaccc attcgagata
     2401 gggaccgaca aaagtccagg cacccatgtt ttttggctcc tcctggcacc aaaagatttc
     2461 ggcttgtttg aaacgcgaca aaatcgcaac aagagctttc aaagggaaag gatagagctg
     2521 ctcgacgcgc aggagataga tatcgttcgc tccccgcttc tcccgttcgt catataggtc
     2581 gtaatagact ttgccggagc ataaaacgac ccgccgaatc tcggcgtctt ctgcgagggt
     2641 gatcggcccg ccgcgtgcgg tttcggcatc atcgagcaag atccgatgga aggacttgcc
     2701 aattacgagg tcctcgagct tcgacaccgc gcgcttgtgg cgtaacagcg attttggtgt
     2761 catgaggatg agcggcttgc ggatatcgcg ttgcagctgg cgccgcagaa tatgaaagta
     2821 attggcgggc gtcgtgcagt tggcaacctg catattgtct tcggcgcaaa gttgcagata
     2881 gcgttcgagg cgggcggacg aatgttccgg cccctggcct tcgtagccat gcggtaggag
     2941 acacacaagg gccgacatgc gcagccattt gcgttcacct gaggaaatga actgatcgaa
     3001 aacgacttgc gcgccattgg cgaaatcgcc gaactgggct tcccacaaca cgagcgcatt
     3061 gggctcagcg agcgaatagc catattcaaa gccaagcaca gccacttccg acaacaagga
     3121 attgatgact tcgaagcgcg cctgcttgtc gcggatattg tttaagggaa tatagcgttc
     3181 ttccgtttcc tggtcgatta ggaccgagtg acgatgcgaa aaggtcccgc gctcactgtc
     3241 ctggccggac agacggacag ggtagccttc gtccacgagc gacgcgaagg cgagcgcctc
     3301 ggccatggcc caatcaatgc ccactccggt ctcaatcatc ttgcggcggt tctcgagcag
     3361 acgccctata gtgcggtgga gacgaaatcc ctccggaacg gctgtgagtt gcaagcccat
     3421 ttcgataagg cgtgcgcggt cgacgcctgt gcggccgcgc cggtcttcct cgattcccgc
     3481 cgccgcgggt ctcagccccg cccagcggcc gtcgagccag tcggctttgt tgggcttata
     3541 ggattgacct gcttcgaacg cggtttcgag gtgatggcgc caattcgctt gcagccgaat
     3601 gacttctccc tccgtaatga ctccttcggc cacaagcttc tcggtataga tttggagcgt
     3661 cgtcttgtga gagcggattt tcttatacat gacgggctgg gtgaacgcgg gctcatcgct
     3721 ttcgttgtgg ccaaaccgcc ggtagcaaaa catatcgatg accaccggct tatggaatct
     3781 ttgccggaat tccgtcgtga ctttcgcggc atagactacg gcctcgggat catcgccgtt
     3841 cacatgaatg atgggtgcct ccaccaattt ggccgaatcc gatggatagg gcgacgaccg
     3901 cgaatggcgc ggtgcggtgg taaagccaat ctgattgttg atgatgaagt gaatcgagcc
     3961 gccagtccgg tatcctttga gcccggagag gccaaaacat tcagcaatga cgccctgacc
     4021 cgcgaaagcc gcgtcgccat gaatgaggag cggcagaact ttcttgcgct ccacgacatc
     4081 gtacaactgg tcctgcttgg cgcgcacctt gccgagaacg acgggatcga cgatctcaag
     4141 gtgggacggg ttcgcggtca gcgaaagatg gactcggttg tcgtcgaagt cgcggtcggc
     4201 ggaggcaccg agatgatatt tgacgtcgcc cgagccttct acatcgtctg gggtgaggga
     4261 gccgcccttg aactcattga aaatggcccg atgcggcttg cccatgacat gggagagaac
     4321 attcagtcgt ccgcggtggg ccatgcccaa cacgatatct tggacaccca aggcgccgcc
     4381 gcgcttgatg atctgctcca gggccggaat catcgcttcg gcgccatcga gtccgaaacg
     4441 cttggtgcct gtgtatttga tgtcgatgaa ttgctcgaat ccctccgcct cgacgagctt
     4501 attgagaatt gcgcgcttgc cctcattggt aaagatgatc tctttgcccg gcccttcgat
     4561 ccgctgttgc agccagccct tctcggccgg atcggagata tgcatgaatt cgtatccgat
     4621 cgtgccgcag taggtacggc ggagaatctt cagcatctcg ggaatggtcg caaatttgag
     4681 cccgagcacg ccatctagga atattttccg attgtaatct ttttcggtaa aaccatacgc
     4741 cgcaggatga agctcctcac gatccttcgg gggttctatg cccagcggat ccagattggc
     4801 atggagatgg ccgcgcatgc gataggcgcg gatcatcatc aatgcgcgca ctgagtcacg
     4861 ggttgcgcgc agcagctcct cggaagaaaa gagtccgcct tcttttgcct tggcctcgag
     4921 cttggcgccg atagcggttt gcgcttctct ccaatttccg tcaagcgccg agatgagctc
     4981 gtccgcagcg gtgggcggaa ggctctgctg cgtccatcgc ggccctcgcg cgcgcctttt
     5041 gattgcaaaa cgatcgtctt tcaactgcgc gaagaattcc cgccaagacg gatcgagcga
     5101 ggcaggatcg cgctcgtaat tgtccgcgag ttgttcgata taggctgcat tagcgccatc
     5161 gagaaaggac gtccgcgcga agccgagatt cggagctgac cgccctcctg gcccgccgcc
     5221 cgcaccattt gcgttaccgt tcgtatcctg ccgcgccatt caattctcct atccgcgaaa
     5281 atctcgccgt aacaatgcac ttccggaccg gtctttgcac attgggggat ctgcgaagca
     5341 ttcggtagcc gaatgaacaa ttcctaccta ttgaaaccca aactatttca tttacgtaac
     5401 agccgagcct gctgctcgta catggtgcaa cgattccact tgtgtcggtt gctcaggcca
     5461 actttgtcac gccacttgcc gggattctgc ttaatacatg cgaacgccag ttggatttct
     5521 caagttctgg ctcatccacg caataaatcg gcaagcgtct tgcctagcct tgccggcgaa
     5581 ggcgacacat aaacgccggc cgcttgcatg gcggcgattt tatcctcagc cccaccctta
     5641 ccgccagaaa tgattgcgcc cgcatggccc atgcgccggc cagggggcgc ggtgcggcca
     5701 gcgataaagc cgacgaccgg ctttttgcgg ccgcgctttg cttcatcggc gaggaattgg
     5761 gcggcttcct cctcggcgga gccgccaatt tcgcctatca tgacaatcga tttggtaaga
     5821 tcgtcggcca ggaacaattc caatatctca atgaattctg tacccttgac tgggtcaccc
     5881 ccgataccaa ccgcggtcgt ctggccaagt ccttcgatcg tggtctgaaa taccgcctca
     5941 taggtcaagg tgccggagcg cgacacaatt ccaaccgaac cgggcttgaa tatatcgccc
     6001 ggcataatgc cgatcttgca ttgcccggcg gtcaccacac ccggagagtt cggtccgatc
     6061 agacgggatt ttgtcccgca cagcgcgcgt ttgaccttga ccatgtccgc gaccgggatg
     6121 ccttcggtga tgcaaacgat aagcggcatt tctgccgcga tcgcctcaca gatcgcgtcc
     6181 gcggctccag acggcgggac ataaattacg cttgcatccg cgcccgtagc gctacgcgct
     6241 gccgcgaccg tactatagac gggcaggccg agatgcgtcg cgccgccttt accgggcgac
     6301 acgccgccaa ccaactttgt gccatagaaa attgcctgtt cggaatggta ggttccgttt
     6361 ttaccggtaa
//
LOCUS       DATMPU010000336         5383 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_252347,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000336 DATMPU010000000
VERSION     DATMPU010000336.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 5383)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5383)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5383
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_252347"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..294
                     /locus_tag="VNY06_07660"
     CDS             <1..294
                     /locus_tag="VNY06_07660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012453661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HWX14710.1"
                     /translation="LFERDPETRAVMMIGEIGGPQEAEAAAWIKSHMTKPVVGYVAGL
                     TAPKGRRMGHAGAIISASGDSAAEKAEIMKSYGLTVAPSPSELGSTMAKILAK"
     gene            494..3193
                     /locus_tag="VNY06_07665"
     CDS             494..3193
                     /locus_tag="VNY06_07665"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012590735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HWX14711.1"
                     /translation="MLDGEETADLLFELLADVVQRKHPEVAAALRGKLEPGELLPDLL
                     SRVFQAHGIWFQLLSIVEQHTATRLRRQTERVRGEAAVESTFSNVIAEAAKLGIDAKE
                     IRDRLASTRVRPVITAHPTEAKRVTVLEKHRRIYLLLVELEQQRWTEGERAGIVDKLR
                     DEIALLWMTGEIRREKPTVDQEVAWGLHFFHETLLEGVPDLLKKLDRALSEHYRGEQF
                     AFTPFFQFGSWIGGDRDGNPFVTNEVTRRALGQNALASLRHYEQHLILLLQRMSIAQW
                     AMPASQAFQAALSNALATSPDGEMIAQRNSGEPYRQFLFAMLRKVQSTIASTSAQQVI
                     DSTGYANADELISDLRILENALVEGGGEDIAKDLVRPLRWAVKVFRFRTVRLDLRENT
                     TRLTQTLHALWQARTGRSRETPPDAGSVEWKNWIVAELARPLPAARPPLDLPPEARET
                     LGMFELAKVNGNDCDREAFGSFILSMTRSVTDILGAYLLAKEAGLFLDSAGVEACSLP
                     IVPLFETIADLRAAPAIMRELLSMPLIRRSAKAQGGVQEVMIGYSDSNKDGGFLSANW
                     ELAKAQARLTKLGNECGVPVGFFHGRGGSVSRGGAPTAQAIAAQPPGSIRGSFRVTEQ
                     GEVVSFKYANRGTAAFQMEVIAAAVLKHALMSEREQAIVPRAEFDDAMEAFSGAAYAA
                     YAQFFSHPDLLEYFQAASPLEEISLLNIGSRPARRFGARSLSDLRAIPFVFAWSQNRH
                     AVTGWYGTGSGIASFLEVRKERGEALLRRMFVESRLFRLILGEVEKTLCLVDLKIARE
                     YASLVADATKRDAIFSLFEMEFALTCEMILRVTREREIGERFPNYRARLAHRLPTINQ
                     VNRDQVELLRRFRACEADEAMESFKVNLLLSINCIASGLGATG"
     gene            3234..4211
                     /locus_tag="VNY06_07670"
     CDS             3234..4211
                     /locus_tag="VNY06_07670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012590736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA ester lyase"
                     /protein_id="HWX14712.1"
                     /translation="MSFTIIEQATPRLHRSELAVPGSNPGMFEKAAKGAADIIFLDVE
                     DAVAPDDKEQARQNIIAGLNETDWGSKTMMVRINGLDTHYMYRDVVDIVEACPRLDMI
                     LIPKVGVPADVYAVDMLVTQIEAAKKREKRIGFEVLIETALGMANVETIAQSSKRLEA
                     MSFGVADYAASTRARTTVIGGVNADYGVLTDQDASGKRAYYWADQWHAAQTRMMVACR
                     AYGLRPIDGPFGDFSDPDGYIAAAKRAAVLGYEGKWAIHPSQIELANQVYTPSEAEVT
                     KARRIVTAMAEAAKAGKGAVSLDGRLIDIASIRMAEALLKKADAIGAAA"
     gene            4593..>5383
                     /locus_tag="VNY06_07675"
     CDS             4593..>5383
                     /locus_tag="VNY06_07675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009735994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HWX14713.1"
                     /translation="MTISAAIHHITHYKYDRPVVLEPQTIRLRPAPHCRTKIPAYSLK
                     VTPAAHFVNWQQDPHGNWQARFVFPEKVTEFKVEVDLTAELAVINPFDFFIEPYAEQF
                     PFAYAPELEAELAPYLDGEPLGPRVAAYIAGLPKGPIHVVTLLVDLNIALQKAIRYVI
                     RMEPGVQPPDETFELGSGSCRDSAWALVQILRYLGLAARFVSGYLIQLRADIEPVDGP
                     QGTQHDFTDLHAWAEVYLPGAGWIGLDPTSGLLAAESHIPLAATP"
BASE COUNT         1080 a         1547 c         1632 g         1124 t
ORIGIN      
        1 cttttcgagc gcgatccgga aacccgcgcc gtcatgatga tcggcgaaat tggcggtccg
       61 caggaagccg aggccgcggc gtggataaaa agccacatga ccaagcccgt ggtcggctat
      121 gtcgcgggtc ttaccgcgcc gaagggccgg cgtatgggcc acgccggcgc gatcatttcg
      181 gcaagcggcg acagcgccgc cgaaaaggcg gagatcatga aatcctatgg cctcacagtg
      241 gcgcctagcc cgagcgagct cggctcgacc atggccaaga ttttggcgaa gtagggggta
      301 tttgcgcggg ccaggtcgag ctgctgtgca attttttgcg cttgcaggca gatgccattt
      361 cccgacgcag cgcgctgagt ttatccaatg gaacggctca gcggctcgtt gccgcgaggt
      421 gactcccatg acttcgttca ggcccggccg gccggggttc gaaatcggca acgcgcctgg
      481 aagcatccag gcgatgctcg atggcgagga aaccgcggac ctgttgtttg aattgcttgc
      541 ggacgtcgtg caacgcaagc atccagaagt cgcggcggcg ctgcgcggaa agctggaacc
      601 gggggaattg ctcccagact tgctgtcccg ggttttccag gcccacggga tttggttcca
      661 gctgctgtcg attgtcgagc agcatacggc gacgcggctg cgccgccaga cggagcgtgt
      721 gcgcggcgaa gcggcagtcg agagcacctt ctccaatgtc atcgccgagg ctgcaaagct
      781 cggcattgat gcaaaagaga ttcgggaccg cctggcctcc acacgtgtcc ggccggttat
      841 cacggcccac ccaaccgaag caaagcgcgt caccgttctt gaaaagcacc gccgcattta
      901 cctccttctc gtcgagctcg aacagcagcg ttggacggaa ggcgagcgcg ccgggatcgt
      961 ggataagttg cgtgacgaga tcgcgctttt gtggatgaca ggggaaatcc ggcgcgagaa
     1021 accgactgtc gatcaggaag tcgcatgggg gctgcatttt tttcacgaga cattgctgga
     1081 gggcgtcccc gatcttttga agaagctcga ccgtgctcta agtgaacact atcgaggcga
     1141 gcagtttgca ttcacaccat ttttccaatt tggctcatgg attggcggcg accgggacgg
     1201 caatcctttt gtgaccaatg aggtgacgcg ccgtgcgctt ggccaaaacg cattggcgag
     1261 cctgcgccac tacgagcaac acctcatttt gctgctgcaa agaatgtcga tcgcccaatg
     1321 ggcaatgccg gcttcgcaag ccttccaggc agccctttca aatgcgctcg caacgagccc
     1381 agacggcgag atgatcgctc agcgcaattc tggcgagccc taccggcagt tcctgttcgc
     1441 aatgctgcgc aaggtccagt ctaccatcgc aagcacctcg gctcagcaag tcattgactc
     1501 gaccggttat gccaatgccg acgaactcat ctccgacctc cggattcttg agaatgcatt
     1561 ggtcgaaggc ggcggcgagg atatcgccaa ggacctcgtg cggccactgc gatgggccgt
     1621 gaaggtgttc cgctttcgca cggtgcggct cgatttgcgt gagaacacca cccgtctcac
     1681 gcagacactg cacgccttgt ggcaagcacg aactggccgc agtcgcgaga cgccgccaga
     1741 tgcgggatca gtcgaatgga aaaactggat tgtcgccgag cttgctcgcc cattgcctgc
     1801 cgcacgtccg cctctggact tgccgccaga agcacgagaa acgcttggca tgtttgaact
     1861 tgccaaggtc aatggcaatg attgcgatcg cgaggccttc ggcagcttca ttctcagtat
     1921 gactcgttcc gtcaccgata ttcttggtgc ctatctcctc gccaaggagg ccggcctttt
     1981 tctcgattcc gcaggcgtcg aggcctgctc tctcccaata gtacctctgt ttgagacgat
     2041 tgccgatctg cgcgccgccc ccgcaatcat gcgcgaactt ttgtccatgc cgctcatccg
     2101 ccgtagcgcc aaggcacaag gaggggtgca ggaagtcatg atcggctatt cggactcaaa
     2161 caaggatggt ggattccttt ctgccaattg ggaattggcg aaggcgcagg cccgattgac
     2221 caagcttgga aacgaatgcg gtgttccagt cgggtttttt cacgggagag gcggttcggt
     2281 gagccgtggc ggagcaccta ccgcgcaagc gattgcggcg caaccgcctg gttcgatcag
     2341 agggagcttc cgggtaaccg agcaaggcga ggtggtttct ttcaaatatg caaaccgcgg
     2401 caccgcagcc tttcagatgg aagtcattgc cgccgcggtg ctcaaacacg cccttatgtc
     2461 cgaacgtgaa caggcaattg tcccaagagc agaattcgat gacgctatgg aagcgttttc
     2521 cggtgccgcc tatgcggctt acgcgcaatt tttttcgcac ccggatcttt tggaatattt
     2581 tcaggcggca agccctcttg aagaaatctc gctcctcaac attgggtcgc ggccagcacg
     2641 ccgttttggc gcgcgttcgc tctctgacct gcgtgccatt ccctttgtct tcgcttggtc
     2701 gcaaaatcgc cacgccgtca ccggctggta tggcacgggg agcggaattg cgagtttcct
     2761 cgaagtgcgc aaggagcgag gcgaggcgtt gctccggcgc atgttcgttg aatcgcgtct
     2821 cttccggcta atccttggcg aggtggaaaa aacattatgc ctcgtcgact taaaaatcgc
     2881 ccgggaatat gcgagccttg tggccgacgc gacgaagcgc gacgctattt tttcgctgtt
     2941 cgaaatggaa tttgcgctga cctgcgagat gattttgcgg gttacgcgag agcgtgagat
     3001 tggcgaaagg tttccaaatt accgggcccg ccttgcccac cggctgccga cgatcaatca
     3061 ggtcaatcgt gaccaagtgg aacttctgcg ccgtttccgc gcttgcgaag ccgatgaggc
     3121 catggagtcc tttaaggtga accttttatt gtccataaac tgtattgcta gcggtcttgg
     3181 tgccactggc tgagaatagc cgcatctgta acgggagagg gaaaggaaag ggcatgagtt
     3241 tcacaattat tgaacaggca acgccgcgcc tgcatcgctc ggagctggcg gttccggggt
     3301 ccaatcccgg aatgttcgaa aaggccgcga agggcgctgc cgacattatt tttcttgatg
     3361 tcgaggatgc cgttgcgccg gacgacaagg agcaggcgcg ccagaacatc atcgccggct
     3421 tgaatgagac tgactggggc agcaagacca tgatggtccg catcaacggt cttgataccc
     3481 attatatgta ccgcgatgtc gtcgatatcg ttgaggcctg cccgcgcctc gatatgatcc
     3541 ttattccgaa agttggcgtg ccggccgatg tctatgccgt ggacatgctg gtgacgcaga
     3601 tcgaggcagc caagaaacga gaaaagcgaa ttggctttga ggtgctgatc gagaccgcgc
     3661 tcggcatggc caacgtcgag acgatcgcgc aatcgagcaa gcgtcttgaa gcgatgtcgt
     3721 ttggcgttgc cgattatgcc gcctcgacac gcgcgagaac aacggtgatc ggtggtgtca
     3781 atgcggatta tggcgtgctc acggaccagg acgcttccgg caaacgcgcc tattactggg
     3841 ccgaccagtg gcatgcggca caaacgcgca tgatggtcgc atgccgcgcc tatggcttgc
     3901 gcccgataga tggtccgttt ggggactttt ccgatcccga tggctatatt gcggcggcga
     3961 agcgcgcagc cgtgctcggc tatgagggga aatgggcgat ccatccctcg caaatcgagc
     4021 ttgccaacca ggtctatacg ccatcggaag ccgaggtgac taaggcgcgc cgcatcgtga
     4081 ccgcgatggc cgaggcagcc aaagcaggaa aaggcgccgt atcgctcgac ggacggctca
     4141 tcgacatcgc cagcatccgc atggccgagg cgcttttgaa aaaagccgat gcaattggcg
     4201 ccgcggccta attgctagcc agggcaagct acggcgagtc gtaccaaatt tttgtccggt
     4261 cgcagcaaaa aatcttacaa ggccacatac gtcttgcggc cttgaggcct ttgggattgg
     4321 gcaaaaaata ggtgtggcag gcaggaccgg agcggcgcct tgcggaatgc caggaggaga
     4381 cgatttgggc ggcatcttgg cgtgaaattt gctggaacca acgccaggaa gcgttgcgct
     4441 tcatgtggcc cgatcgagga tcgggcgagc ttcgtcctat cttcaagttt tcggttcgag
     4501 accatgatgt tttaccccgt ccatgaatcc cggcggcggc agggtggctt cgcgtagcgg
     4561 cgcgctacag gcgtcgtttg ggattttgca acgtgaccat ttccgccgct attcaccaca
     4621 tcactcatta caagtacgac cgaccggtcg tgctcgagcc gcagaccatc agactgcgcc
     4681 cggccccgca ctgccgtacg aagatcccgg cgtattcgct gaaggtgacg ccggccgcgc
     4741 atttcgtgaa ctggcagcaa gacccgcacg gcaattggca agcgcggttc gtgtttccgg
     4801 agaaagtgac ggaattcaag gtcgaggttg atttgaccgc cgagcttgcg gtcatcaatc
     4861 ctttcgactt tttcatcgag ccctatgccg agcaatttcc gtttgcttat gcccccgaat
     4921 tggaggccga gcttgcgccc tacctcgatg gagagccgct cggtccgcgc gttgcggcct
     4981 atatcgcggg cttgcctaag ggccccatcc acgtcgttac tttgctcgtg gatctaaata
     5041 tcgcgttgca gaaggcgatt cgttacgtga tccggatgga gcccggcgtc cagccgccgg
     5101 atgaaacctt cgagcttggc tcggggtcct gccgcgattc ggcctgggcg ctcgttcaga
     5161 tcctgcgcta tcttgggctc gcagcgcgct tcgtctcagg ctacctcatc caattgcgcg
     5221 ccgatatcga gcccgtcgac ggaccacaag gcacgcagca cgatttcacc gatctgcacg
     5281 cctgggcgga ggtctatctg cccggcgccg gctggattgg gctcgatccg acctcgggcc
     5341 tgttggccgc ggaaagccac attccgctgg ccgcaacgcc gca
//
LOCUS       DATMPU010000337         2618 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_2135749,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000337 DATMPU010000000
VERSION     DATMPU010000337.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 2618)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2618)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2618
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2135749"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..542
                     /locus_tag="VNY06_07680"
     CDS             <1..542
                     /locus_tag="VNY06_07680"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HWX14714.1"
                     /translation="STHKLQAANDAGAHAVIDASGENWFAAARRYAPNGYQAVFDANG
                     LTLRQSLRLLAPMGRLVAYGSHAIVPRNGKLIGALATVMRYPLLPRFGVLRLANENKS
                     VMAFNLSYLFDEASLLDRIMGNLLTDFDQGRLVPPPTRCYPFEQAGLAHRDVQSGQTI
                     GKCVLTMGPVEANSRNRPS"
     gene            complement(747..1184)
                     /locus_tag="VNY06_07685"
     CDS             complement(747..1184)
                     /locus_tag="VNY06_07685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX14715.1"
                     /translation="MSSPTTPPEKGVPLGRILIILSVFAFPGLVYLGVRHLGDRIKVV
                     DIPIVGEWQAPGKPWRIVFRPDKTLVSSTSPSQSGPGTYSVDYFGTLWVKLNNGKIYS
                     AALAAESPDRFDLIESDTLVPTVFQKVPPPTPKPPDSPKESPG"
     gene            1767..2258
                     /locus_tag="VNY06_07690"
     CDS             1767..2258
                     /locus_tag="VNY06_07690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012589567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family protein"
                     /protein_id="HWX14716.1"
                     /translation="MFPDAFLSRAADLIASCRTKGLTVATAESCTGGLLAALITAIPG
                     SSDVFERGFVTYSNAAKIECLGVSPRILEDFGAVSGEAARALAKGALAHSSADITLAV
                     TGIAGPGGGSPGKPVGLVHFGLAHRGGAIVGVEKRFGDLGRDGVRSAAIAIAIELLLE
                     AAC"
     gene            complement(2300..>2618)
                     /locus_tag="VNY06_07695"
     CDS             complement(2300..>2618)
                     /locus_tag="VNY06_07695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012589566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HWX14717.1"
                     /translation="LTLIADMEIGYKAIRETFTSRVTCERSVFKILVEYIDGPFKHLE
                     NRWIFHDRDEGRACLVDFRIAYEFKSRALSLIMGGMFDAAFRKFAAAFEDRADIVYGK
                     GAS"
BASE COUNT          519 a          742 c          801 g          556 t
ORIGIN      
        1 ggtccaccca taagcttcag gcggcaaatg atgcgggcgc acatgcggtc atcgatgcat
       61 caggcgagaa ttggttcgcg gcagctcgcc gttacgcgcc gaatgggtac caagccgtct
      121 tcgatgctaa cgggctgacc ctgcgccaga gcttgcgatt gttggcacca atggggcgac
      181 tggtcgcata cgggtcgcac gcgattgtac cgcgcaacgg aaagctgatc ggcgcgttag
      241 cgaccgtgat gcgttatccg ctcctgcctc gttttggtgt tctgcggctc gccaacgaaa
      301 acaagagcgt catggccttc aatttgagct atttattcga cgaggcctcc ctcctcgatc
      361 ggatcatggg caatttgctg acggatttcg atcaagggag gctggtcccg ccacccacga
      421 ggtgctaccc gttcgaacaa gcaggtctgg cacaccgcga tgttcaatcc gggcagacga
      481 tcggcaaatg cgtgcttacc atggggcccg tcgaggcgaa ttcgcggaac aggccttcgt
      541 aatgatgggt gatatgcgct ttttcgcggt aggttgggta gcgaagaaga agccggtgca
      601 aagttggcgg atatcgtccg cagaccgatc gcggcaagac cgcgcgccgc cggtctttgg
      661 attggctcga cgtgaaactc cggcctaacc ggatcgtcca atcgcttggc gttacccttg
      721 cggaaagcga gagctaggga cacgcattag cccggggatt ccttcggcga atccggcggc
      781 tttggcgttg gcggcggcac cttttggaac acggtcggca ctagagtgtc agattcaatg
      841 aggtcgaacc ggtcgggact ctccgctgcc aaagccgccg agtaaatctt gccattattc
      901 agctttaccc acagcgtgcc gaaataatcc accgagtacg tccccggtcc cgattgcgaa
      961 ggactcgtgg aactcacaag cgtcttgtcc ggtcgaaaca cgatgcgcca gggcttgccg
     1021 ggggcttgcc actccccgac gatcgggatg tcgactactt tgatcctatc gccgagatgc
     1081 cgaaccccta agtagacgag gccagggaag gcgaagacac tgaggatgat gagtatccgg
     1141 ccgaggggta cccccttttc tggcggtgtc gttggtgaac tcatcgcaac ctctcaacaa
     1201 gctgggcggg agagtagccg catatataga taaacggcaa gataagctca agggggccga
     1261 tgcgggctgg tgtctcattc caaagttacg atgatttcag cttctggcaa cttccgccgc
     1321 gaggagactt ttggctcttg gtcgcggacg cagcgagaag aactcgctac ccgcgccgca
     1381 agctgacgtg gttttgcggt agtgcatcag tttgaatccg ctctggtact ggtaaagatt
     1441 ggggaattat gcacttgaca agttatggcg gattggcctc atcgtagcca gatgagaatg
     1501 ctgcttttta tgttgccatt tgtccttttg accgcctgcg aacgggatga tcctgtgaag
     1561 cgctgccaac gggaatttgg cacacaaggc caacgtgtcg tcgatgactg cgtgcgctgg
     1621 tctatcgcgc cgccggcagg tcaacctgaa aaaggtcaac ctcgagattg atggcacctc
     1681 gcgagagcaa gacgaagctt ccagtccggt agctggcgtg cccgtccgtg gtttcgggcg
     1741 tatcccctcg ggtcgggaaa cgcggcatgt tccctgacgc attcttgagc cgcgcggcgg
     1801 acctcatcgc atcctgccgc accaaggggc ttaccgtggc aaccgccgag tcatgcacgg
     1861 ggggcctctt ggcggccttg atcaccgcca taccgggatc gtccgacgtt ttcgagcgcg
     1921 gcttcgtcac ctactccaat gccgcgaaaa tcgaatgtct cggggtctcg ccgcggattt
     1981 tggaagattt tggcgcggtc agcggagagg ccgcacgggc cttggctaaa ggcgcgctgg
     2041 ctcattcctc cgcggacata acgcttgcgg tcaccggcat cgcggggccc ggcgggggtt
     2101 cccctggcaa gcccgtcggc ctcgtgcatt ttggcctcgc acatagaggc ggcgccatcg
     2161 tcggcgtcga aaaacgtttc ggagaccttg gccgcgacgg cgtgcgttcc gccgcgatcg
     2221 ccatcgcgat cgagctgctc ctcgaagcgg cttgttgatg cacgcgcggg gggagaattt
     2281 tcgatcgctc gggctcgcgt catgacgccc ccttgccata gacaatatcg gcgcgatctt
     2341 cgaaagctgc ggcgaatttt cgaaaagccg cgtcaaacat tccgcccatg atgagactca
     2401 gcgcacggct cttgaattca taggcaatgc ggaaatcgac gaggcaggcc ctgccttcgt
     2461 cgcgatcgtg aaagatccag cggttttcga gatgcttgaa cggtccgtcg atatattcga
     2521 caagaatttt gaacactgag cgctcgcagg taacgcgact cgtgaaggtc tcgcggattg
     2581 ccttgtaccc gatctccata tcggcgatga gcgttagt
//
LOCUS       DATMPU010000338         5045 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_2737409,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000338 DATMPU010000000
VERSION     DATMPU010000338.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 5045)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5045)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5045
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2737409"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            327..515
                     /locus_tag="VNY06_07700"
     CDS             327..515
                     /locus_tag="VNY06_07700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX14718.1"
                     /translation="MLHDELLKRGHDLFAVVDRQTNFARCKPLKSFIDLQLRPLDFAK
                     FVRHPRVFAALVESNETS"
     gene            623..1372
                     /locus_tag="VNY06_07705"
     CDS             623..1372
                     /locus_tag="VNY06_07705"
                     /inference="COORDINATES: protein motif:HMM:NF021365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04282 family arsenosugar biosynthesis
                     glycosyltransferase"
                     /protein_id="HWX14719.1"
                     /translation="MAKASYPGQAKTRLCPPLTFEEAAAFNTAFLKDTADNLIAVSAD
                     ASIAGSMAYGPPGSEAFFRANLPPAIVLHEVWFPDLGAGLIETMRKLFAAGNRVACVL
                     NSDSPTLPAALLAEMASVLAEPGDRAVFGPCADGGYYLLALKSLRRRLFEDIAWSTPI
                     VAQQTLERAAEIGLPMHILPEWYDVDDCEGIRLLMGELLEGQPYSRSLRSNPARHSLA
                     LLKSMVSNSNLRERLDAFTPPRPAKLEGAVG"
     gene            1369..2676
                     /locus_tag="VNY06_07710"
     CDS             1369..2676
                     /locus_tag="VNY06_07710"
                     /inference="COORDINATES: protein motif:HMM:NF021134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase 87 family protein"
                     /protein_id="HWX14720.1"
                     /translation="MRGFPNFMIAAGLLSASMVPGAAALFAKKLETVVYLALAQGVIY
                     AVFAFLLLRERYALTERQSRRYFILLLALGTGMRAMLLFAPPHSSDVYRYVWDGRVQA
                     YGINPYRYIPADPALAKLRDEDIYPQINRSEYAPTIYPPAAQAVFFAVSRLSENVTIM
                     KAAMLAFEALAVWALIRLLAARGLPVILVSLYFLHPLAVSEIAGSGHVDSVAVAFLML
                     ALLAAEKGNCFGSGAALAAGALTKYFPLALAPAIYRRWDWRMPAAFVAAAVTLYLPYL
                     SVGKKVTGFLSGYADEELGEENGLYILAILKQLGLGAAALPLFLGAGGLTLLALAWRA
                     GFRPCPSKPDPQGAFAIAICCTVLFTPHYAWYFLWLVPFLCFFPRPSVFWLTLSAPLL
                     YPIAWPPTVAGFSIQYVPFAVLLAVENLRFFNAKEAPRGRAVA"
     gene            2657..3739
                     /locus_tag="VNY06_07715"
     CDS             2657..3739
                     /locus_tag="VNY06_07715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020096393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HWX14721.1"
                     /translation="MGAQLPESKPIEAAKLDPRRYFERIREKRTPIAADDPVCLYLET
                     TNRCNLLCTTCPRTYEELEPPSDMSWELFTSIVDQFPKIERVVLHGVGEPMMVAELPK
                     MVKYLKDRGVYVLFNTNGTLMREKKSRELIEAGLDEMRISLDAAEPKAFEAVRGRDLF
                     ARILRNVKNFVALKERMGAARPRLSLWLTGLRETLEQLPAFIRLAHELGVPEVHLQRL
                     VYFESNPIGLARPESSLFESHDAIDGKYLLQAENIAAELGVMFDASGAGDPETSLRRI
                     EGDAPWSLCRRPWSLMYFTAHGRALPCCIAPFSMHGYENFTLGDAKTTLLKNIWNGPR
                     YRSFREALLSESPPKACAGCGLRWSL"
     gene            3739..4533
                     /locus_tag="VNY06_07720"
     CDS             3739..4533
                     /locus_tag="VNY06_07720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020096394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HWX14722.1"
                     /translation="MSDGSMPFPPDEVRGMCVIIPVLNEEEAIAAVLKRIPRGVADEI
                     IVADGGSKDRTVEIALECGTRVLNLGRGYGLACMEGTRAASDGCQILIYMDGDGSDFP
                     EEIPKLVAPILAGTHDLVLASRTGGKREPGSMAWHQVASGWLIGALAGLLYGFRYTDM
                     CAFRAIRRDGLIALGMRELSYGWNLEMQMKAARFGLRILEIPVPYACRTGGRSKVAGS
                     LSGTLRAGSRILLTFVRVALHSERRQPEEVELRRRQIISFKSPAKH"
     gene            complement(4816..>5045)
                     /locus_tag="VNY06_07725"
     CDS             complement(4816..>5045)
                     /locus_tag="VNY06_07725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX14723.1"
                     /translation="GAPSLRMQVAMSAVLAASGVLVLILIIALSNPFRGDFRVSTAPF
                     ELSRSRRGMAAWNYCCPPVASIPYNAANGSL"
BASE COUNT          953 a         1475 c         1500 g         1117 t
ORIGIN      
        1 ctgcaagcat ttgaatttgc tcgttgatta ggagcgagaa tcgcagaaaa aggcccaaat
       61 ttgaaaaatt ccctgttaaa ttccctgtta gcagggaatc tcgcttcttc ccaagcgcgc
      121 cgtcagctcc atgagttcag gggtggcccg gtccgggcaa atttcgccga gaaccgtgaa
      181 cggcatcgaa atgtgggaaa ggcaacgctg acaaagcatc cagcgcgggt ttttcacagc
      241 gacggtccca aacacggtcc gaattttccg catcgtgtag tccttaaccg gccgcaacct
      301 ttcacaggag ggacagacgc gagctcatgc ttcacgacga actcttgaag cgcggacatg
      361 atctttttgc cgtcgtcgat cgacagacca atttcgccag atgtaaacct ctcaaatcct
      421 ttatcgatct gcaattgcgc ccgctggatt tcgccaaatt cgtccgtcac cccagagttt
      481 ttgccgctct cgtcgaaagt aacgaaacct cgtgagggaa cgaaagcgtt agtaagaaca
      541 tttttagggc tagattatcg aatcgcttgg cgtgaatgtg agagcgcagg aatacccggc
      601 cgccttttgc agcatcgcag ttatggccaa ggcctcctat ccaggccaag ccaagacgcg
      661 gctttgcccg ccgctgactt tcgaggaagc ggcggcgttc aataccgcgt tcttgaagga
      721 caccgccgat aacctgatcg cggtttccgc ggatgcgagc atcgccggat ccatggccta
      781 cggccctccc ggcagcgagg ccttctttcg tgcgaacttg ccgcccgcaa ttgtcctgca
      841 tgaagtctgg tttccggatt tgggcgctgg ccttatcgaa acgatgcgaa agctgtttgc
      901 ggctggcaac cgggtcgcat gcgtcttgaa ctcggacagc ccgaccctcc ccgccgccct
      961 gcttgccgag atggcgagcg tcctggctga gcctggagac cgggccgttt tcggaccctg
     1021 tgccgacggg ggctattatc tcttggcgct caaaagcttg cgccggcggc tttttgagga
     1081 tatcgcctgg agcacgccaa tcgtagccca gcagacgctg gagcgggccg ccgaaatcgg
     1141 gcttcccatg catatcctgc ccgagtggta cgatgttgac gattgcgaag gcatccgcct
     1201 gctcatgggc gagttgctcg aaggccagcc ttactcgaga tcgctgcgct ccaatccggc
     1261 ccggcattct ctggcgcttc ttaagtccat ggtctccaac tccaatttga gagagcgcct
     1321 tgacgcattc acaccgcccc gcccagcaaa gttggaaggg gctgtgggat gagagggttt
     1381 ccaaacttca tgattgcggc cggcctactg tcggcatcga tggtaccggg cgctgccgcc
     1441 ctttttgcca agaagctcga gacggtcgtt tatctcgcgc tcgcgcaagg cgttatttac
     1501 gcggtcttcg cattccttct cttgcgcgaa aggtatgccc tcacagagcg gcagagccgc
     1561 cgatacttta tcctcctgct tgctcttggc acggggatgc gcgcgatgtt gctgttcgca
     1621 ccgccgcatt cctcggacgt ctatcgttat gtttgggatg gccgcgtgca ggcgtacggg
     1681 atcaacccct atcgctacat tccggccgat ccggcgctcg ccaaactccg cgacgaggac
     1741 atctacccgc aaataaaccg gagcgagtat gcgcccacca tctatccgcc agccgcgcaa
     1801 gcggtatttt tcgcggtcag ccgcttgagt gaaaatgtca ccattatgaa ggcggcgatg
     1861 cttgccttcg aggcgctcgc cgtttgggcg ctgatccggc ttcttgctgc gcgtggcttg
     1921 ccggtcattc ttgtgtccct ttactttctc caccccttgg cggtatcgga gattgccgga
     1981 agcggccatg tcgacagcgt ggcagtcgcc ttcctcatgc tagccctgct tgcggcggaa
     2041 aaaggcaatt gctttgggtc cggcgcagcc cttgcggcag gcgcactcac aaaatacttt
     2101 ccgctcgctc tggcgccggc gatctaccgc cgttgggatt ggcggatgcc tgcggcattc
     2161 gtcgcggcgg ctgtcacgct ttatctgccc tatctttcag ttggaaagaa agtgaccggc
     2221 tttcttagcg gctatgcgga cgaagagctc ggggaggaaa acggcctcta tatccttgca
     2281 atcttaaagc agcttggctt gggtgcggcg gcgctgccgc tgttcctagg cgccggcggg
     2341 ttgactctct tagcactcgc ttggagggca ggattccggc cctgtccaag taagccggac
     2401 ccgcagggcg ccttcgccat agccatttgc tgcacggtgc tcttcacgcc ccattacgct
     2461 tggtacttcc tttggctcgt tccattcctg tgcttctttc cgcggccgtc agtgttttgg
     2521 cttacgcttt cggcgccgct tctttacccc atcgcttggc ccccgaccgt cgcgggattc
     2581 agcattcaat acgtgccgtt tgcagttttg ctggcggtgg aaaatcttag gttcttcaac
     2641 gcaaaggagg cgccccgtgg gcgcgcagtt gcctgaaagc aagcccatcg aggctgccaa
     2701 actggacccg cgccgctact tcgagcgcat cagagaaaag cgcaccccca tcgcggcaga
     2761 tgatcccgtc tgcctctatt tggaaacgac caatcgctgc aatctcctat gcaccacttg
     2821 cccgcgcact tacgaggagc tcgaaccgcc ctcggacatg agctgggagc tgttcacatc
     2881 cattgtcgat caattcccca agatcgagcg ggtcgttttg catggggttg gcgagccgat
     2941 gatggtggcc gagctgccca agatggtgaa atacctcaaa gaccgaggtg tttacgtgct
     3001 gttcaacacc aacggtacac tgatgcgcga gaaaaagagc cgggagctga tcgaggcggg
     3061 cctcgatgag atgcgcatct cgctcgatgc ggcggagccg aaggcgttcg aggcggtcag
     3121 gggccgcgat cttttcgcgc gaatcctgcg taatgtgaag aacttcgttg cgctgaaaga
     3181 gcggatggga gcggcaaggc cgcgtctttc gctttggctc acaggtttgc gcgaaaccct
     3241 tgagcagctg cctgcgttca tccgactcgc acatgagctt ggggtgcccg aggtccacct
     3301 tcagcggctc gtatatttcg aaagcaatcc gatcgggctg gcgcgtcctg agtcctcgct
     3361 gttcgaaagc cacgacgcca tcgatgggaa gtatctgctt caggccgaga acatcgcggc
     3421 agagctcggc gtcatgttcg atgcgtctgg ggctggcgat ccggagacta gcctgcgccg
     3481 gatcgaaggc gatgcgcctt ggtcgctttg ccgccgcccg tggtcgctga tgtatttcac
     3541 cgcgcatggg cgcgccctgc cgtgctgcat cgcgcccttc tccatgcatg gctatgagaa
     3601 cttcacgctt ggcgatgcca agaccacgct gcttaagaac atctggaatg gaccacgata
     3661 tcgaagcttc cgtgaagcac tcctgagtga gagcccgccc aaggcttgtg cgggctgcgg
     3721 cttgcgctgg agtctgtgat gagcgatggc agcatgccct ttcccccgga tgaagtgaga
     3781 ggaatgtgcg tcatcattcc agtcctcaac gaggaggaag cgatcgctgc tgtcttaaag
     3841 cgcatccccc gcggcgtagc ggacgagatc attgtcgccg atggcggctc gaaggaccgc
     3901 acggtagaaa ttgcattgga gtgtggcacg cgggtcctta atctaggcag agggtatggc
     3961 ctggcctgca tggaaggtac gcgcgccgct agcgatggct gccagattct catctatatg
     4021 gatggcgatg gcagcgactt tccagaggag ataccaaagc ttgttgcccc gatcctcgcc
     4081 ggaactcatg atttagtgct ggcctcgagg acaggcggga agcgtgagcc cgggtcgatg
     4141 gcctggcatc aggttgcgtc aggctggctg atcggcgccc ttgccggatt gctttacggc
     4201 ttccgttaca ccgacatgtg cgctttccgg gcaatccgcc gcgatgggct gattgcgctt
     4261 ggcatgcggg agctgagcta tggctggaac ttggaaatgc agatgaaggc agcccgattt
     4321 gggctgcgaa ttctggagat tccggtgcct tacgcctgtc gaactggagg gcgttcgaag
     4381 gttgccgggt cgttgagcgg cacgctccgt gccggcagcc gcatcctgct gaccttcgtc
     4441 cgagtcgcgc tccattcaga acggcgccag cctgaagagg ttgagcttag acggcgccag
     4501 atcatcagtt ttaagtcacc cgctaagcat tagcggttgc ttgtttgagc ctgtcgctca
     4561 gaacggtcat gctgtagcgt tcaaccacgt cgggctcacc gcgtattact ttgaattccg
     4621 tttcttcgcg ttcccctcag aaactgatgt caagctgatg cttgcccaca cgaaggccgt
     4681 acacggtgag atcgggcagc caggctggca gtagcgggtc gatgtagagt ttgccgtttg
     4741 gggcgtccgc catgaagccg agtatgatct cgtgcccgaa caggaatgct tcattccttg
     4801 cctgtgggtc atgagtcaga gcgatccgtt agccgcattg tatgggatgg aagccacggg
     4861 gggacaacaa taattccaag cggccatgcc gcgccgactg cgcgacagct cgaatggcgc
     4921 ggtcgagact cggaaatcgc cgcggaacgg gttgctgagg gctattatca ggatcagcac
     4981 caggacgccg gaggcggcga gcacagccga catcgcaacc tgcatccgca ggctcggcgc
     5041 accaa
//
LOCUS       DATMPU010000339         5180 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_256412,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000339 DATMPU010000000
VERSION     DATMPU010000339.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 5180)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5180)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5180
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_256412"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..439)
                     /locus_tag="VNY06_07730"
     CDS             complement(<1..439)
                     /locus_tag="VNY06_07730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX14724.1"
                     /translation="MAIASNDYQGLEVVFDQYLFPYCINCFSDANERTRAVAFLRRYW
                     FDPGSPDAFFRGFPVARIYAEGVLKALELSLNGRRVVPLNAWWILDTTEPKMLVLADV
                     DNQGVTVGGRVTLLVVTPRPSGRVRPRGAAILGQTAQAWVSERQ"
     gene            complement(618..4013)
                     /locus_tag="VNY06_07735"
     CDS             complement(618..4013)
                     /locus_tag="VNY06_07735"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HWX14725.1"
                     /translation="MTEVARWLREIGLAQYADLFDERSIGFESLKSLTEQDLKQLGLP
                     PVPSQVILREIELLASISPASVAVPAPLPPDTAERRQLTIMFCDLINSVPLSTRLDPE
                     DLRDVITAYHRTCAQSVRRYNGFVARYMGDGILIYFGYPAADEDDAERAVRAGLKLVD
                     AVAELNNSMKLFTHLNLQVRVGIATGLVVVGDEIAENVLDKDAIAGEAVNLAARLQTL
                     AEPNTVVTSAATRQLAAERFAYRELGLREIKGFETPVPVYQVIGEREVSRLAARSAAL
                     TPFVGRDKEIELLLARWARAISGDGQVMILAGEAGIGKSRIAAEVCSQIRRNNPEIPA
                     PFFFQCSPHHTNAPLYPVIKALERAAEISRSRPPAENREKLKTLLKIDERDDDRRLAL
                     IVDLLDLALDGDPKPFALSASAKRGLTIEALNDWVARCTQETPSILVFEDVQWIDPTS
                     KLLLNRLIDWAKTARALIVITLRIESSLAERWCEEAGLSTIGSQRQSYLTICEVGELS
                     AGEATCLAGAAAEGRAIPDRQLSDILKKSEGIPLYLEELVRAVVNPVDFSPNHEEADQ
                     TDAVPNTLRDALMAQLDRLGRAKELAQHASVVGQEFSASLLAKVANKPIEDLLPDLHS
                     LINSKIVMQAGATSDIYRFKHALLRDISYRSLLRKVRRQIHLSVAGELANPTTMAIDA
                     TDDLIAQHYSRGTAYSEAIQFWQKGAKAAIARSAHEEALAMLHAARMDLRRLPAAEWR
                     SAVELDLVFAQAVALRSLRGYSAPEVEERLLEARELCSMCADDSNRFNVEWALFQCTI
                     VKRDIEAARQLAAGLFEHAKRHPDVPLVDAYLANGMVAVNAGEFETARRFLEKGVSLS
                     RPETDQPHFFAHGQNPGLFCLSYLARTWCFLGYLDCGRAVMNQCLSIAANRAHEPGHV
                     YGYVNALIHAVRVYNLCGDLDAEERLAEETNEISRRNHFAYYEAQSECHLAWVAGARG
                     LLAEGIQRMVGGIAALGETATSLALPGFYVLLAQLYIRAGQWDEARTVLQMAVGARGH
                     AVWDADVERVRGDLLSLQVGSDLSAAETAYRASLTIARRQRAGLLEFKAGLHLAHLLR
                     DLHRPKEGFEILSVCLEKLHGEFGTADARNARILMNDLAVTDH"
     gene            4369..4728
                     /locus_tag="VNY06_07740"
     CDS             4369..4728
                     /locus_tag="VNY06_07740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX14726.1"
                     /translation="MNSTRPASGQGSSTEKSELTRHLDSIGWAAFFIWIGIAMLLAIP
                     WGWFLVGLGILILAAQLARLQMNIKIEGFWVTWGAVFLIGGLWTVLNLASWPLAPIVL
                     ILLGVVLLAKAIMSIRR"
     gene            4817..5167
                     /locus_tag="VNY06_07745"
                     /pseudo
     CDS             4817..5167
                     /locus_tag="VNY06_07745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611896.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
BASE COUNT         1091 a         1534 c         1435 g         1120 t
ORIGIN      
        1 cttgtcgctc ggatacccac gcctgcgcgg tctgacccag gatcgctgcg cccctgggtc
       61 tcactcggcc gcttggtctg ggtgtcacaa ctagcagggt cactcggccc cccaccgtta
      121 ctccttgatt gtccacgtcc gccaagacca gcatctttgg ttctgtcgta tcgaggatcc
      181 accaagcatt gagcggaaca acgcgtcgcc cgttcaatga taattcaagg gcctttagca
      241 ccccctcggc atagattcgg gcaaccggga agccccggaa gaatgcgtca ggcgagccgg
      301 ggtcgaacca atatcgcctc aagaaggcaa cggccctggt tctctcattg gcgtcgctaa
      361 aacagttaat acagtatggg aataaatatt ggtcaaacac cacctccaac ccctgataat
      421 cgttggaggc aattgccatt atcgcattcc tcagtcgggt gagccgcgtc cgagcccccc
      481 gccccttaga aaaatcgtcc aaacaacagc agacgatccc cttggcacat ggtacgccag
      541 ttgatacagg atcagccaat cattcctccc ttaacggccg ggcattctcg cgcgctacaa
      601 atggacgcgc ttccggtcta gtgatccgtg acggcgagat cgttcatgag tatgcgcgca
      661 tttcgcgcat cagcagtgcc aaattcgcca tgcagctttt cgagacatac cgagaggatc
      721 tcgaagcctt ctttgggacg atgcaagtct cgcagcaagt gggccaagtg cagtccggcc
      781 ttgaactcca gtaaaccagc ccgttggcgt cgtgcaatgg ttaaactcgc ccggtatgcg
      841 gtctcggccg cggacagatc ggagcccacc tgtaatgaca acaagtcgcc ccgcactcgt
      901 tcgacatcag catcccacac cgcgtggccc cttgctccga ctgccatttg cagcaccgta
      961 cgcgcctcgt cccattgacc ggcacggata tacaattgcg ccaaaagcac gtaaaaaccg
     1021 ggaagcgcca gcgaagtggc cgtctctccg agcgcggcga taccgccaac cattctctga
     1081 attccctccg ccaggagccc cctggcgccc gcgacccaag ctaggtgaca ctcactttgc
     1141 gcctcgtagt acgcgaagtg attgcgccga gaaatttcgt tggtttcctc ggccaatcgt
     1201 tcttcagcgt cgagatcgcc gcacaaattg taaactcgga ctgcatgaat aagcgcgttg
     1261 acgtaaccgt aaacatgccc gggctcatgt gctcggttgg ctgcgatcga aagacactgg
     1321 ttcatcacag cccggccaca gtccaaatat ccaagaaaac accatgtgcg agctaaatac
     1381 gataggcaga atagccccgg attttgcccg tgggcaaaga aatgtggttg atcggtctcc
     1441 gggcggctaa ggctgacgcc tttttcaaga aagcgtctgg ctgtctcgaa ctccccggca
     1501 ttgaccgcga ccattccatt agcaagatag gcatcgacta acggcacatc agggtggcgc
     1561 ttggcgtgtt cgaaaagacc ggcagctagt tgacgcgcgg cctcgatatc gcgtttgacg
     1621 atagtacact gaaacagcgc ccattcgaca ttgaaccggt tgctgtcgtc ggcgcacata
     1681 gagcaaagct cacgagcctc aagcaaccgc tcctcaacct cgggcgcgga atagccgcga
     1741 agcgaacgca gcgctaccgc ctgagcaaag accaaatcaa gttcgacagc agagcgccac
     1801 tccgcggctg gtaatctccg taggtccatt cgggccgcat gcaacatggc gagcgcttct
     1861 tcgtgcgccg atcttgctat ggcggcctta gcaccttttt gccaaaattg gattgcttcg
     1921 gaataggctg tacctcggga atagtgttgg gcaatcaggt cgtcggtggc atcgatggcc
     1981 atcgttgtcg gattcgcgag ttcgcccgca acgctgaggt gaatttgccg gcgcaccttt
     2041 ctgagcagcg accggtagga gatatcccgc aacagcgcat gcttgaaacg ataaatatcg
     2101 gaggtcgccc cagcctgcat tacgatcttt gaattgatca ggctatgcag gtcggggagc
     2161 aaatcttcga tgggcttgtt cgcgactttg gccaacaagc ttgccgagaa ctcttggccg
     2221 actaccgaag catgttgtgc taattctttc gcgcgcccaa gccgatcgag ttgcgccatc
     2281 aatgcgtccc gcagcgtatt cggtacggcg tctgtctgat cggcctcttc gtgatttggc
     2341 gagaaatcca caggattgac aactgccctt accagctctt cgagatacaa gggaatcccc
     2401 tctgacttct ttagtatatc gctcaattgg cggtcgggaa tcgccctgcc ctcggctgcc
     2461 gcccccgcca agcacgtcgc ctcgcctgcg ctcagttcac caacttcgca aatggttagg
     2521 tacgattggc gttggctgcc aatagtggag agcccagcct cttcgcacca tctttccgca
     2581 agcgagctct cgatacgcag cgtaattaca attaaggctc gtgccgtctt tgcccaatcg
     2641 atcaaccgat tgagcagtag tttgctagtc gggtcgatcc actgcacgtc ctcgaacacg
     2701 aggatcgacg gtgtttcctg cgtgcaacgc gcaacccagt catttagcgc ctctatcgtg
     2761 agtcctctct tggcgcttgc acttaacgcg aaaggcttcg ggtctccgtc tagggcgagg
     2821 tccaacagat cgacaatcaa agcaagccgc cggtcatcgt ctcgctcgtc aatcttaagc
     2881 agggttttca gcttttcccg attctctgcc ggtggccgag accgactgat ctccgccgcc
     2941 cgttctaacg cctttattac cggataaagg ggagcgttcg tgtggtgtgg cgaacattga
     3001 aagaaaaacg gcgctggtat ttcggggtta tttcttcgaa tttgagaaca cacctctgcg
     3061 gcgatacgcg attttccgat acctgcttcg cccgccagga tcatgacttg accgtcgccg
     3121 gaaatcgcgc gcgcccaacg ggcaagaaga agttcaattt ctttgtcccg accaacgaac
     3181 ggagtgaggg ccgcgcttct cgccgcgagc cgactgacct cccgctcgcc gatgacttga
     3241 tatacgggaa ctggggtttc aaagcctttg atctctcgca agcccagctc gcgatacgcg
     3301 aacctctccg ccgccagctg ccgtgtcgcg gctgaggtga cgacagtgtt gggctcggcc
     3361 agcgtttgca gccgggccgc aagattgacc gcctccccgg cgatagcgtc cttgtccaag
     3421 acattttcgg ctatttcatc gccgacaacg accaaccccg tggcgatgcc gacgcggacc
     3481 tgaaggttca gatgggtaaa gagcttcatg ctgttgttca gctcggcgac ggcgtccacg
     3541 agcttaagcc cggcacgcac agctctctcg gcgtcgtcct catctgccgc cgggtagccg
     3601 aaatagatca gtataccgtc ccccatatac ctcgcgacaa agccattata gcgccggacc
     3661 gattgcgcgc aggtccggtg gtacgcggta atgacgtccc gcagatcttc ggggtcgagc
     3721 cgcgtcgata aaggaacaga atttatgagg tcgcagaaca taatagtgag ctgccgccgc
     3781 tcggccgtat cgggcggcaa gggagccggc acggccacag acgcgggcga aatcgaggca
     3841 agcaactcaa tttcacgtag gattacttgg ctggggacag gtggaagccc caattgcttc
     3901 agatcttgct cggttagact tttcaaactc tcaaaaccaa tgctccgttc gtcaaacaga
     3961 tcagcgtatt gcgcgagacc tatttctctc agccaacgtg ctacctcggt catgaagatc
     4021 ccccgaggac gtgtttcctg aatttattct taacaccttg gagccgatca ttctatcaag
     4081 ccggttgccg gtggcaccca gggaatttcg ggaatattac cgcggtctct gattaacctt
     4141 tatcatggtg aatgaggaag cgagttgcca ggcggcggcc cgggattgcg cgcgaggagc
     4201 ggtttcgaac gatctaattc tcgcgacggc tcagactaac aggccgggtt tgggttcgca
     4261 cagcggggtg ccggtttcgg cgaggcggcg caggagcgcg gcgggggccc agcgctcgcc
     4321 atattgctgg tgccaggcct ggatctttgt ttgggagcaa ggaggacgat gaacagcacg
     4381 cgaccggcct ccggtcaggg atcgtccaca gaaaagagcg agcttacccg gcatctcgat
     4441 tctattggtt gggctgcctt cttcatttgg atcggtatcg ccatgttact ggcgatacct
     4501 tgggggtggt ttctcgtggg ccttggcatt ctcatcctgg ccgcacaact tgctcgcttg
     4561 cagatgaaca tcaagatcga aggcttctgg gtcacctggg gggcagtgtt tctcattggc
     4621 gggctatgga cagttcttaa tttggcttct tggccactgg ccccgatagt tcttatcctg
     4681 ctgggagtgg tgctactcgc taaagccatt atgagtatca ggcgctaacc tcaaatattt
     4741 ggtcatgtcg cctcgaagca gatgcacatg caagcgattt ccggtgttgg gggaggctca
     4801 cccggctgac gaggcgctga gcgaggccct cgtccggttc ggcaatcccg gcatcttcaa
     4861 taccgatcaa ggggcgcaat tcaccagcga cgagtttacc aagatgctgc gggatcacgg
     4921 gatcgcgatc agcatggacg gccgcgggcg ctgccacgac aacatcttcg tcgagcgctt
     4981 gtggtggacc gtgaagcacg aatgggtcta cctgcgcccg gccgccgacg gcatcgaaca
     5041 gaagcgcagc cttggcgcgt ttttcgactg gtataatctg cgacgtccgc atcaggcgct
     5101 cggctggcga acacccgacg aggcttacct cggccaatcg acggctgcgt cggcactggc
     5161 cgcctgacgc gatgccgaga
//
LOCUS       DATMPU010000340         6818 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_3363498,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000340 DATMPU010000000
VERSION     DATMPU010000340.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 6818)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6818)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6818
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3363498"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..785)
                     /locus_tag="VNY06_07750"
     CDS             complement(<1..785)
                     /locus_tag="VNY06_07750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020175654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0033573 - high-affinity iron permease
                     complex [Evidence IEA];
                     GO_function: GO:0005381 - iron ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034755 - iron ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FTR1 family protein"
                     /protein_id="HWX14727.1"
                     /translation="MAGAFIIVLREVIETGLIIGIVLAVTRAVPRREFYISGGIIVGL
                     LGAALIAALAGALSNALAGAGQEVFNASVLGIAVIMLGWHNVWMARHGRQISENVRQI
                     GRDVAAGARSMMALAVVITVTVLREGSEIVLFLYGLVISSREAGAGLLLGGLLGLLAG
                     ASISALAYMGLAVIPPRYLFKITGVLIGFMAAGMAAQSIAFLEQANIVTHLGGTVWNT
                     SWILSDNGITGRVLHTLIGYTGKPTQMQLLVYLATLGTIFGLK"
     gene            complement(1031..1525)
                     /locus_tag="VNY06_07755"
     CDS             complement(1031..1525)
                     /locus_tag="VNY06_07755"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020177163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HWX14728.1"
                     /translation="MGFATLARQPSAKTKICLRPLKSADLSGLAELWVASWQEAMPGI
                     DFVARRRWFFDHMRTLHAAGSVTVCAVDGSGSIVGFVSVDPISAYLDQLAIAPAAKGT
                     GVARLLLNEARRISPKGIVLEVNEDNQRALAFYKREGFVQISEGVNPLSGLKTLRLRR
                     PGHS"
     gene            1732..1905
                     /locus_tag="VNY06_07760"
     CDS             1732..1905
                     /locus_tag="VNY06_07760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX14729.1"
                     /translation="MTGIGGILGEIAGMSRRDGVSFPDVTLEQDVFLRNVIFLQILVL
                     SRVLTQNWFPFLA"
     gene            2121..3815
                     /locus_tag="VNY06_07765"
     CDS             2121..3815
                     /locus_tag="VNY06_07765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012589950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HWX14730.1"
                     /translation="MTPVGVEDFAIYLARKSCLLPDARSRSDTPASTSASAERSLRKL
                     WELTELSASGFADEVARFYGLRRLSLPELVAASVLTERFSSRFLRDSAVLPFEQPKGQ
                     FKLAVADPTDGASVRAAEIVLGGPVTVEVASFEDIATVLDQQLGSEEPPAAGSGETAR
                     AGTDDDIESLRDLASGAPVVRAVNDMIERAMELRASDIHIEPIRNGLVVRMRVDGLLR
                     AVAAPLGVLPQALISRIKILASLNIAERRLPQDGAARLRVARSEIDIRVSTMPTQHGE
                     SAVIRLLPRDRGLLDIAKLGFSGADEKTVRRLLALPHGMIILTGPTGSGKTTTLATML
                     SILNEPSRKILTIEDPVEYEIQGINQSQVKPSIGLTFAAAMRAFVRQDPDVIMVGEVR
                     DPETANIAVHAALTGHLVLTTLHTETAAAAVPRLLDLGVEGFLLKSTLRAVIAQRLVR
                     ILCDRCKKERVLNESDIANDRRYETLGFQPRNRVYEPAGCERCGGTGYRGRCGVFELL
                     ELKDEVYELVRPEADGRTIDLAARRAGMTTMVEDAVAKCRAGVTSTAEVLRVTTVR"
     gene            3819..5033
                     /locus_tag="VNY06_07770"
     CDS             3819..5033
                     /locus_tag="VNY06_07770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014493807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HWX14731.1"
                     /translation="MTNFRYRAMTRNGEVVNGSISASTAAEVAERVEYLGLIPIETIR
                     DDGARPGQRLDIAFLSQPRPEDVTVFTGDLALLLQTGARINDALELLSSDADIGRLQA
                     TVGKITSAILAGESFADALERHPALFSPMYIELARVGEASGTLVNILEVLAAERVRAE
                     ALRRRFSDALRYPAFVFAAASAVLLFFLTFVLPQFGDVLRDFNAKLDPIIVGFLALSD
                     FLRAHTSAIFASLIITIGGGWALSRQPRVRSAILDMFAHVPFISRILNFHRTSVFCRN
                     LGVLLSSGVPLTMTLRILADLMATTGSSAAWTKTVDLVRHGGKLSEALAKTEAIPPMA
                     IRTLRLGEDSGQLATLAQRVAEFYEAKLQRSLDRLVGIAGPVAIVVISIIVGGLIVSV
                     MTALMSVSQIVG"
     gene            5050..5583
                     /gene="gspG"
                     /locus_tag="VNY06_07775"
     CDS             5050..5583
                     /gene="gspG"
                     /locus_tag="VNY06_07775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012114134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system major pseudopilin GspG"
                     /protein_id="HWX14732.1"
                     /translation="MNVFKLTNVFHTRRRGDRRNRYQSENDRHRILCGGRSSTSREDG
                     FTLVEILVVITIIGLIMGLVGPRVLNYLSESKVKAATIQIQSFTSALDLFYLDLGRYP
                     STSEGLAALIQRPAGSSSWNGPYLKSSTVPNDPWGHPYKYRSPSEHGPFEIISLGPDG
                     KEGRTGTAANIKSWEMR"
     gene            5580..6041
                     /locus_tag="VNY06_07780"
     CDS             5580..6041
                     /locus_tag="VNY06_07780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015664739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HWX14733.1"
                     /translation="MTVPQGTSGFTLLEMVCVLAIIALLLATALPALPRGTSRPMLEA
                     YALRTAALLKADRAAAIRRGVPVATELSPMARMIRSGANGRSLQLPQDVGFRAILASQ
                     CGARAAGTTIQFLPSGMSCGGTIYLSRPGVTLEVRITWLTGGVEIVPGSDS"
     gene            6019..6441
                     /locus_tag="VNY06_07785"
     CDS             6019..6441
                     /locus_tag="VNY06_07785"
                     /inference="COORDINATES: protein motif:HMM:NF019575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HWX14734.1"
                     /translation="MSLAATHKRAAGSSGAEAGFTLIEALVAVAVAAVTLTAIAALMG
                     GNIRGAGKIARHVQLSATLRAVEAALPNRAALGTENLTGEMHGQNWSVEVVPFPHDFV
                     NPRAAALWTPQEIVIAVQSPTGGQIRLETVRLTKRIGQ"
     gene            6441..>6818
                     /locus_tag="VNY06_07790"
     CDS             6441..>6818
                     /locus_tag="VNY06_07790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX14735.1"
                     /translation="MTRKQAPMPLLRRVDGFTLLEALLAVGLMTLLLATLATVTAQWM
                     RGWKAGFDSTQSADLLGLGLDRIVADIGAAEFISPGQADTRPLFQGTDSSVTFVRSAI
                     GPNSPLGLEVVRLAEIDDARGRIL"
BASE COUNT         1443 a         1951 c         1918 g         1506 t
ORIGIN      
        1 ttcttgagtc caaagatcgt tccgagagta gcgaggtaaa ccagaagctg catctgggtc
       61 ggcttgccgg tgtagccgat aagcgtatga agcacgcgcc cggttatacc gttatcgctc
      121 aaaatccagg acgtgttcca cacggtgcca cctaggtggg tcacgatgtt ggcttgctcg
      181 agaaaagcaa tgctttgcgc agccattccg gctgccatga acccaatcag cacgccggtt
      241 atcttaaaga gatagcgcgg cggaatgact gcaagaccca tataggcgag cgcgctgatc
      301 gaagccccgg ccaaaagccc gagcaaaccg ccgagaagta gcccagctcc ggcctcgcgg
      361 ctagaaatga ccagtccata aagaaacaag acgatttctg aaccttctcg gagtaccgtc
      421 acagtgatga cgactgcgag cgccatcatc gatcgcgcac cggcagcgac atcgcgaccg
      481 atctgccgga cattctcgct gatctggcgc ccgtggcgag ccatccagac attatgccag
      541 ccaagcatga tgaccgcgat gccaagaaca gaggcattga aaacttcctg gccggcacct
      601 gccagcgcgt tggaaagcgc gccagcaagg gccgcgatca gagccgctcc gaggagtccg
      661 acgataatcc ctccgcttat ataaaattcc cgccgcggga cggcgcgtgt cacggcgagc
      721 acaatgccga tgatcaaccc tgtttcgata acttctctca ggacgatgat gaatgcgccg
      781 gccacgtgaa tatccctgcc tccaagttcg cgctgaccca agcgggcgcc gacgctcgtc
      841 tcttggaacc cctgtcacat cggctccagc gtcccatcgc caatcctttg aggaactata
      901 ccaagacctt cgatccatca acgaattctc cgttaattgg aacgattaca atgagcggac
      961 aggttaaatc acggaaatct cgcaatggtg cttttaccct gtctccttta gcccgaatcg
     1021 catgctggga tcaggaatgt cccgggcggc gcagccgtaa tgtttttaag ccagagaggg
     1081 ggttcacacc ttctgagatc tgcacaaatc cttcccgctt gtagaacgcc agagcgcgtt
     1141 gattgtcctc attgacttca aggacaattc ccttcggaga aatcctgcgc gcttcattaa
     1201 gaaggaggcg tgccactccc gtacccttcg cagctggcgc aatagccagc tgatcgaggt
     1261 aagccgagat cgggtcgacg gaaacaaagc caacaatcga acccgagcca tcaacggcgc
     1321 aaactgtaac agagcctgcc gcatgaaggg tccgcatatg atcgaaaaac catcggcgcc
     1381 gagccacgaa atcgattccg ggcatggcct cctgccagct tgccacccaa agttcggcaa
     1441 ggcccgacaa atcggcactt tttagggggc gcaaacaaat tttcgtcttt gccgaaggtt
     1501 gccgagccaa ggtggcgaaa cccattatct ggtttgacga gcggccaagg ttgaaacgcg
     1561 aacagccacc tgaagcatcg atcagcagac ggaaaaagtc tcgccgagga tgcgacttat
     1621 cccacagaag atgtgaacgc taggcgtaca caatctgcgc gctttcaaac gcgagcacga
     1681 gacctccgtc acgataaaat cccagaaaag gggtaagaat tgcattgggc ggtgacaggc
     1741 atcggcggaa tccttggtga aatcgctgga atgagccgac gcgacggcgt ctcctttccg
     1801 gatgttactc tagagcagga tgtctttctt cgaaacgtca tcttcctcca gatccttgtt
     1861 ttgtcgcgcg tcctgacgca aaattggttc ccgtttttgg cttaatcctt tgctttgatc
     1921 gcatgaatcc agtttctggc cggctcttgt ggcctggctt ttctgctgca attagccagc
     1981 ggcttgaggg aatggttgca gactttttgg acaaaatcat gcgacaaaca ggtataaaag
     2041 ctggcggcgg ttatgtttta aggcatcagg ctctttggcg ccctgatagt aaaatggctc
     2101 ggattctttg agacatgcaa atgaccccgg tcggtgttga agatttcgct atatatttgg
     2161 ccaggaaaag ttgtctgctt cctgacgcgc gctcgcgctc ggataccccc gcctccacct
     2221 cggccagcgc ggagcgatcg cttcgcaaac tttgggaatt gaccgaactt tcagcgagcg
     2281 ggtttgccga cgaagtcgcc cgattttatg ggctaagacg gctttccttg ccggaactcg
     2341 ttgccgcatc ggttctgacc gagcggtttt cttcacgctt tttgcgagac agtgcggtct
     2401 tgccattcga acagcccaag ggccaattca agcttgccgt cgctgaccca acagatggcg
     2461 cctccgttcg tgccgccgaa atcgttctcg gcggccctgt caccgtggag gtagcgtcct
     2521 ttgaagatat tgcgaccgtt ctcgaccagc agctcggcag tgaggagccg ccggctgccg
     2581 gcagcggtga aacggcgcgt gctggtacag atgatgacat cgaaagcctg cgcgatcttg
     2641 cgagcggtgc gcccgtggta cgtgcggtaa atgacatgat cgagcgggcg atggagcttc
     2701 gcgcgagcga cattcatatt gagccgatcc ggaacgggct tgtcgttcgc atgcgggttg
     2761 acggcttgct ccgcgctgtg gccgcgccgc tcggcgtctt gccgcaggct ctgatttccc
     2821 gaatcaagat actggcgagc ctcaacattg ccgaacgaag attgccgcaa gacggggcgg
     2881 ctagactgcg ggtcgcgcgc tcagagattg atattcgcgt ctccaccatg ccaacccagc
     2941 acggcgagtc agccgtcatt cgcctgttgc cgcgtgatcg cggtttgctt gatattgcca
     3001 aactaggttt ctcgggtgcc gacgagaaaa ccgtgcgccg cctgcttgcc ctaccgcatg
     3061 gcatgatcat cctgaccgga ccaactggca gcggcaagac gacgaccctt gcgaccatgc
     3121 tctcgatcct gaacgagcca tcgcggaaaa ttctcacaat cgaagaccct gtggaatatg
     3181 aaattcaagg gatcaatcaa tctcaggtga aaccttcgat cggccttact tttgccgccg
     3241 cgatgcgggc attcgtccgg caagatccgg atgtgatcat ggttggagag gtacgcgatc
     3301 cagaaaccgc gaatattgcc gttcacgccg cgcttaccgg gcaccttgtg ctcaccacgt
     3361 tgcataccga aacagcagcg gccgccgtgc cgcgcttgct cgatctcggc gtggagggtt
     3421 tcttgcttaa atcgacatta cgggcggtga tcgcccaacg tttggtccga attttatgtg
     3481 atcggtgcaa gaaagaacgc gtcctcaacg aaagcgatat tgcgaacgat cgccgctatg
     3541 agacgcttgg gtttcagccg aggaaccgcg tctatgaacc agctggttgt gaacgttgcg
     3601 gcggcactgg ataccgcggg cgctgcgggg tttttgaact gcttgaatta aaggatgaag
     3661 tttatgagct tgtccggcca gaagcggacg ggcgcacgat cgacctcgcc gcgaggcgtg
     3721 ccggtatgac gactatggtc gaggatgctg tcgccaaatg ccgcgccggt gtcacctcta
     3781 ctgccgaagt cctacgtgtt acgaccgtga ggtgagccat gacgaatttc cgctaccggg
     3841 cgatgactcg caacggtgaa gtcgtcaacg gttccatttc agcctccacc gccgccgagg
     3901 ttgctgagcg tgtcgaatat cttggcctca ttcccatcga aacgatccgc gatgatggtg
     3961 ctcgccctgg ccagcgcctc gacattgcat ttctgtcgca gccgcgtccc gaagatgtga
     4021 ccgttttcac tggcgatctc gccttgctgc tgcaaaccgg cgcgcgaatt aatgacgccc
     4081 tcgaactttt atcgtctgac gcggacattg gacgcctgca ggcaaccgtc ggcaagatca
     4141 cctcggccat tctcgcgggc gagagctttg ccgatgcgct ggagcgtcat ccagccttgt
     4201 tttcgccgat gtatatcgag cttgcacgcg ttggagaagc atctggcacg ctggtgaaca
     4261 tattggaagt gttagctgcc gaaagggtca gggccgaggc cctgcgccgt cgcttctccg
     4321 atgccttgcg ttacccggcc tttgtgttcg ccgccgcaag tgccgtgctg ctcttcttcc
     4381 taactttcgt gcttccgcag tttggcgatg tattgcgcga cttcaatgcg aaacttgatc
     4441 cgatcatagt tggatttctt gctctttccg attttctgcg ggcgcacact tcagcgatct
     4501 tcgcgagcct cataataaca attggcggcg gatgggcgct ttcgcgccag ccaagggtgc
     4561 gcagcgcaat tttggacatg ttcgctcatg tgccttttat aagccggata ttaaattttc
     4621 atcgaacgtc ggtcttttgc cgtaaccttg gcgtccttct ctccagtggg gtgccattaa
     4681 cgatgacgct gcggattctc gccgatttga tggcaacgac tggcagctca gccgcctgga
     4741 cgaaaacggt ggatctcgtt cgccacggcg gcaagctgtc cgaagcgctg gccaagacag
     4801 aggcaatccc gccgatggcc attcgtactt tgcggctcgg cgaggactct ggccagctgg
     4861 ctacgctcgc gcaacgcgtc gccgaatttt acgaagcgaa gctccaacgc agcctcgacc
     4921 ggcttgtcgg tatcgctggc ccggtcgcga tcgttgttat aagcatcatt gtcggcggat
     4981 taatcgtgtc cgtcatgacc gccctcatgt cagtcagcca aatcgtcgga taaaaaggga
     5041 ttagtctcca tgaatgtatt taagttgacg aatgtatttc atacgcggcg ccgtggtgac
     5101 cggcggaata ggtatcagtc ggaaaacgat cggcatagga ttttatgtgg cggccgaagc
     5161 agcaccagcc gcgaggatgg cttcaccctc gttgaaatct tggtcgtcat caccatcatc
     5221 ggcttaatca tgggcctcgt cggaccgcgg gtcctaaact accttagcga gtccaaggta
     5281 aaggcagcga cgatccagat ccaaagtttt acgagcgcat tggacctttt ctatctcgat
     5341 cttggacgtt atccttcgac cagcgaaggc ctcgctgcac ttatacagcg cccggctggt
     5401 tcttctagtt ggaacggccc ctatttgaag agttccactg taccaaatga tccttggggt
     5461 catccctata aataccgttc tccaagcgag cacggccctt ttgaaattat ctcgctcggg
     5521 cccgatggta aggaaggtag gactggcacc gcagccaaca taaagagctg ggagatgcga
     5581 tgacggttcc ccagggtacg agcggtttta ctttgctcga aatggtttgc gtcctcgcta
     5641 tcatagcact actgctggcc acggccttac cggctttgcc gcggggaacc tcgcgcccga
     5701 tgcttgaggc ttatgccctg cggacggcgg cgttgctcaa ggccgatcgt gccgcagcga
     5761 tccggcgcgg cgttccggtt gccacggaat tgagccccat ggcccggatg atccggtccg
     5821 gcgccaacgg acgctcgttg caattgcctc aagatgtcgg atttcgagct atattagcga
     5881 gccagtgcgg cgcacgcgcg gccggtacaa ctatccagtt cctgccatcc ggaatgtcct
     5941 gcggcggaac aatttacctc tcccgacccg gcgtaaccct cgaagtccgt ataacttggt
     6001 tgactggggg agttgaaatt gtccctggca gcgactcata agcgtgccgc cgggagttcc
     6061 ggcgccgagg cggggttcac ccttatagag gcccttgtcg ctgtcgccgt cgccgcagtc
     6121 accttgaccg caatcgcagc gctcatggga ggaaatattc gcggagctgg aaaaattgcg
     6181 cgccatgttc aacttagcgc gactttaaga gctgtcgaag ctgcacttcc aaatcgcgcg
     6241 gctttgggga cggaaaactt aaccggtgaa atgcacggcc aaaattggtc cgtcgaggtc
     6301 gtgccatttc cgcacgattt cgttaatccg cgcgcggccg cgctgtggac gcctcaagaa
     6361 attgtgatcg cggtgcagtc gccgaccgga gggcaaattc ggctagagac agtccgtctg
     6421 acaaaaagga ttggtcagta atgacacgca agcaagcgcc gatgccgctt ctccggcgcg
     6481 tagacggatt cacccttctg gaggcccttt tagcggtcgg cctgatgacc ttgcttctcg
     6541 cgacgcttgc tacagtgacg gcgcaatgga tgcgcggctg gaaggcggga ttcgacagca
     6601 cccaaagcgc cgatctgctc ggactcggcc tcgatcggat cgttgccgac attggagctg
     6661 cagaattcat atccccaggc caggcggaca cacgcccctt atttcaagga acggattcct
     6721 cggtaacttt tgtgcgttcc gcgattggcc caaattcacc gctaggacta gaagtcgtgc
     6781 ggctcgctga aatagacgat gcgcgcggcc gcatattg
//
LOCUS       DATMPU010000341         3134 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_1808825,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000341 DATMPU010000000
VERSION     DATMPU010000341.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 3134)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3134)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3134
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1808825"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(251..496)
                     /locus_tag="VNY06_07795"
     CDS             complement(251..496)
                     /locus_tag="VNY06_07795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012384695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX14736.1"
                     /translation="MSDAYVIQVRGCTAGIVARDAQGHGFNFFSAAPRFNVMEGQHFS
                     DPSAAECAARKLAKHGSLPRRARGRPFAAGNRDAPGL"
     gene            complement(774..1706)
                     /locus_tag="VNY06_07800"
     CDS             complement(774..1706)
                     /locus_tag="VNY06_07800"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012589225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HWX14737.1"
                     /translation="MKILAGNSNRALTEAIGAYLGLPLTKCHVRRFADMEVFVEILEN
                     VRGQDTFIIQSTSYPANDHLMEVLIMTDALRRASARRITAVIPYFGYARQDRKPGPRT
                     PISAKLVANLITRAGADRVLTVDLHAGQIQGFFDIPTDNLYAAPVLVRDIKERLELRN
                     LMVVSPDVGGVVRARALAKRIEAPLAIVDKRRERAGDSEVMNIIGEVAEKSCVLVDDI
                     VDSGSTLCNAADALLREGAEVVYAYITHGVLSGGAVARIAASRLKEMVITDTIQATEA
                     IRVSNNIRVVSIAPLIGEAIARIAKEESVSSLFD"
     gene            complement(2059..2598)
                     /gene="greB"
                     /locus_tag="VNY06_07805"
     CDS             complement(2059..2598)
                     /gene="greB"
                     /locus_tag="VNY06_07805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012589216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HWX14738.1"
                     /translation="MSKAFTKEPEAEDFDDEVRALAAGTKNYITPEGLERLRDEVARL
                     QKFERPKTVETVSWAAGNGDRSENGDYIYGKRRLREIDRRLRLLRKRMEITEVVDPAR
                     QMNRDRIYFGAKVVYRNSRSEQKTVRIVGIDEARSDHGEVSWISPVAKALLKAAVGDV
                     VEVRTPKGLESIEVLKILY"
     gene            complement(2666..>3134)
                     /locus_tag="VNY06_07810"
     CDS             complement(2666..>3134)
                     /locus_tag="VNY06_07810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX14739.1"
                     /translation="PNKARVAAAAKKGAAKRNMAQAGANTVSKSKPAEPVTVAAAGPA
                     EPAKPESSSFFSRLWGAKPTEAEAAAPPDASVSATSAAASEPPPAKVPLPPKRKEPST
                     SGAKPADPKAKLQNSVAAPAPVRPHAALSPSQAVSKTLAMLRPGLSTNTQAQQ"
BASE COUNT          641 a          925 c          897 g          671 t
ORIGIN      
        1 gcgcgccgtt tcgagattgc cgtcacaacc ttccagcgcc gttacttcgg atcgggtcag
       61 cacctcgata ttcgctgtca gcttgagtga ggccgaaagc cgcagcgttc tcgacgagcg
      121 cgcgctggtc tgcggtcggc acagattcgg ctggtatttc ccgttccgtg tcgctcaact
      181 aagggacgcg cgcgcgagag gcgggaaact aagggatctt ttctttgtgg cagcttttgg
      241 gaaatgtgct tcacaagccg ggagcgtcgc gattaccggc ggcaaagggc cgccccctcg
      301 cgcggcgcgg caggctgcca tgtttggcaa gtttccgcgc tgcgcactcg gcggcggaag
      361 gatcggagaa atgctggccc tccatgacat tgaagcgtgg cgcggcggag aagaagttaa
      421 agccgtggcc ctgcgcatcc cgggcgacga ttcccgcggt gcagccgcga acttggatga
      481 cataagcgtc agacataaat gctcctcaac tcgggacttt gcgaccgtaa tttgccgaga
      541 cgctctctat gcttacccaa caaagattgt cctcgtcctt cagtccttca ccttctgacg
      601 ctcaagggcc cggccttccc gccgccaatc tcaactggtg acgggtggaa cgtttgcaag
      661 gcataaaaac cttgcaacaa tctttggcag cagtgtgagc ttggcttggc gcttttgcga
      721 aagtaataga cgctgcactt ttgactaagc atgcaacgtg ccactttcgc cccttaatca
      781 aagagactgg agacgctttc ttcctttgcg atccgggcga tcgcttcgcc aatcaaaggg
      841 gcaattgaaa cgactcgaat attgttggaa acccggatcg cttccgttgc ctggatggtg
      901 tcagtgatga ccatttcctt gagccgtgag gcagcaatgc gcgccaccgc gccaccagag
      961 agaacgccgt gagtgatata ggcatagact acctctgcgc cttctcgcag caaggcgtcg
     1021 gcggcattgc acaatgtgct tcctgaatcg acaatatcgt caacgagtac gcaacttttc
     1081 tccgcaactt cgccgataat gttcatgact tccgagtcgc ccgcccgttc gcggcgctta
     1141 tcgacaatgg cgagcggcgc ttcgatacgt ttggctaagg cgcgagcgcg cacgaccccc
     1201 ccaacatcgg gcgagacgac catcaaattc cgaagttcca gccgttcctt gatgtcccgc
     1261 accaggacag gcgcggcata gagattatcg gtcggaatat cgaagaagcc ttgaatttgg
     1321 cccgcgtgga ggtcgacggt aagcacacgg tccgcacctg ccctggtaat aagatttgcg
     1381 acgagtttgg ccgagatcgg cgtgcgcgga ccgggtttgc ggtcctgccg cgcatagcca
     1441 aaatagggta tgaccgcggt aatgcgccgg gcagaggcac gccgcaatgc gtcggtcatg
     1501 atgagtactt ccatcaaatg atcattcgcc ggataggacg tcgactgaat aatgaaggtg
     1561 tcctgaccgc gcacattctc gagaatttcg acaaaaacct ccatgtctgc gaagcggcga
     1621 acgtggcatt tggttaacgg caagccgaga taagcaccga tcgcctccgt cagagcccgg
     1681 ttcgagtttc ccgctaggat cttcatcgcc gccgccttcc gtcggtccct tgctttgcac
     1741 tcgtgaaagc cgatcgtaag agccaccctg ggtaacgccc atggcagcgc tgcgaaccct
     1801 atagctcata ctttggtgcg tagcacaatc cccaacgctc agcccttcgc aaccaaagga
     1861 gcggaaaaga tccacggaaa ttgcttgcca gaatccaaag ccgcagccat ctcaaagagc
     1921 ggccagtcaa gccagccatt tgtgtgcctg gcaatgaaat ctgtggcgct tcgcactctg
     1981 cggttcgggg acttgagaac gcctgagaga ttggccagca attcgcagcc gggcaggaac
     2041 catcgcaaga cgcgctgctt aatagaggat cttcaagacc tcaatagact caaggccttt
     2101 gggtgtcctg acctcgacaa catcgccaac cgcagccttc aaaagcgcct tggcgaccgg
     2161 cgatatccag ctcacctcac catgatccga ccgcgcttcg tcgatgccca cgatccggac
     2221 cgtcttttgc tcgctgcggc tgtttcgata aacaactttt gctccaaaat agatacggtc
     2281 gcggttcatc tgccgtgcag gatcgacgac ttcggtgatt tccattcttt tgcgcagcaa
     2341 gcgcagcctc cggtcgattt cacgcaggcg ccgtttgccg taaatgtaat ctccattctc
     2401 cgaccggtcg ccattgcccg cggcccagga aacggtctca actgtcttgg ggcgctcgaa
     2461 tttctgcagc cgcgcaacct catccctcag ccgctccaga ccttccggag taatgtaatt
     2521 cttagtccca gccgccagcg cgcggacctc gtcgtcgaaa tcctcggctt cgggttcttt
     2581 ggtaaaggct ttgctcatgg cgctgaccag ctaatgtggg gcgccacggg acggaggcaa
     2641 gccccgacag gcaatgataa gaaaattact gctgagcctg cgtgtttgtc gagagaccgg
     2701 gccgcagcat tgcgagggtt tttgaaacag cctgggaagg cgacaatgca gcgtgaggcc
     2761 gaacaggtgc cggggctgcc acggaattct gtagtttagc cttggggtcg gctggtttag
     2821 ccccagacgt cgagggctcc ttacgctttg gcggcagggg cactttggcc ggcggaggct
     2881 ctgaggcggc agcagaggta gctgagaccg aggcatctgg cggcgctgct gcctctgcct
     2941 ctgtcggttt cgcaccccat agccggctaa agaaggaact gctctcgggc ttagctggct
     3001 cggcaggacc ggctgcggcg actgttacag gctcagccgg cttggatttg gaaactgtat
     3061 tggctccggc ctgcgccata tttcgcttgg cggcgccctt cttggccgca gccgcaactc
     3121 tggctttgtt cggc
//
LOCUS       DATMPU010000342         4265 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_2419003,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000342 DATMPU010000000
VERSION     DATMPU010000342.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 4265)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4265)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4265
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2419003"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            228..989
                     /locus_tag="VNY06_07815"
     CDS             228..989
                     /locus_tag="VNY06_07815"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: protein motif:HMM:NF014641.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006782 - protoporphyrinogen IX
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HWX14740.1"
                     /translation="MLILVTRALDESQRTAASLAREGHDSVVSPVTEMVPTGAVWPAG
                     VIDGVIATSARAFELFSCAPDWPLPEARRLIRLLLVGGRTLDAARERGFAGTALTAPD
                     ATALGTKIELHFSSPRRLVYLAGRDRNPGLENKLTDLGHAIELVEVYAAQPADSLTED
                     ALALAGQGKVGAVLHYSRRSAEIYLRLARNAGLDLSRVNHVCISHNAAAPLLAAGIHE
                     VLVAKASNEQAMFGLVNALAGLPETPLGQAEKLDS"
     gene            1036..2232
                     /locus_tag="VNY06_07820"
     CDS             1036..2232
                     /locus_tag="VNY06_07820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018267141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWX14741.1"
                     /translation="MIRHIGLTALVVALLSLAFPPVFAADIVEVKTGFIRLAHLQETV
                     SILDSPAADDGIAGAKLAIEDNNTTGRFLGQTFAIEDVVLNPDGDPIAALDGLVKRGI
                     FLLIAELPAALLLTLSDAAKDKGVLIFNIGAPEDSLREENCRANVFHIAPSYSMLADG
                     LAQYLVWKQWKRWFLMEGSHPEDTLFAGALERAAKKFGAKIVEERVYQDTGGGRRSDS
                     GSVQTQKLIPVATQNAPAYDVLLAADESEVFANYLPYRTWDPRLVAGSAGLKPMSWDG
                     SHEQWGAIQLQNRFMKLAFRRMNARDNNAWVAMRMIGEAASRAGSNDPKIIRSYLTGP
                     DFAIAAFKGQKQTLRRWNQQLRQPILLGDGRTIVSVSPQEGFLHQTSQLDTLGFDEPE
                     TRCHLE"
     gene            2217..3236
                     /locus_tag="VNY06_07825"
     CDS             2217..3236
                     /locus_tag="VNY06_07825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018267142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YVTN family beta-propeller repeat protein"
                     /protein_id="HWX14742.1"
                     /translation="MPSRVIFQEGLKACALTHVPLRLAFIALACVTAFQAQAFTAYVS
                     NEKGNSISVIDTDKMEVTETILVGHRPRGIALSHDGGQLFICAGDEDLIQILDTKRRS
                     IVGTLPSGPDPELLVLSPDGKLLYVANENDNMVTAIDVATRKIAAEIPVGVEPEGMEL
                     SPDGKVLVNTSETTNMAHFIDTHTHKIFANVLVDARPRVAQFTPDGLELWVSSEVGGT
                     VSVIDAGNPKVKQKITFDVPGLSKEAIQPVGLRFTQDGRTAFVALGPANRVAVINAAT
                     KSIEKYLLVGQRVWQMAFTPDSKFLYTTNGVSNDVSVIDVASLKVLKSIPVGSFPWGV
                     AISRQ"
     gene            3233..4027
                     /locus_tag="VNY06_07830"
     CDS             3233..4027
                     /locus_tag="VNY06_07830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012589063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWX14743.1"
                     /translation="MSFSTPEPEAAIGRPSRLTLALEIGCVSYSYGKRKALEDVSFSV
                     APGSFTVLLGLNGAGKSTLYCLITRLYATQQGRIRIFGFDVNRSSGEALRRLGVVFQA
                     RTLDLDLTVLQNLSYHAALHGISTKETKRRANLVLEMIALTERKNDKARNLSGGQMRR
                     VEIARALLHRPQLLLLDEPTAGLDVKARADILMHVRNLAAQEGIGVLWATHLIDEVEA
                     ADDVVILHHGKVLDSGKASDIAGWAVATEFRAAFDKMTAASGKAQQ"
     gene            4039..>4265
                     /locus_tag="VNY06_07835"
     CDS             4039..>4265
                     /locus_tag="VNY06_07835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018408761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug ABC transporter permease"
                     /protein_id="HWX14744.1"
                     /translation="MARPGYRGYSIVNYARCFKGIVWRETLRYWHQRERFLSALVRPL
                     VWLFIFAAGFRSVLGVSIIPPYETYVLYEVY"
BASE COUNT          894 a         1175 c         1207 g          989 t
ORIGIN      
        1 aatggccagc atgatgcaat agctgtccgt agcgcacagt cattcattca tgcaccaaca
       61 gttgtctgac gtttgatgat tctctctcaa tcgtcgtcgc tggacaaagt tctgttcaat
      121 ttgtgtgtta tgcgcctcat caacgatctt tccttgccgg ccatgatgga cgctggcagg
      181 gtgtcacgtg gcaccccacg aaacgtttgt gaaagctgac tcggacgatg ctcatattgg
      241 tcacacgggc gcttgatgaa tcgcagcgga ctgccgctag ccttgcgcgc gaagggcatg
      301 acagcgtagt ttcgccggtg accgaaatgg tgccgaccgg agccgtatgg ccggcaggtg
      361 ttattgatgg cgtgattgca acgagcgcgc gtgcgttcga attattctct tgcgcgccgg
      421 attggccatt gcctgaggca cgccgcctca ttcgcttgct tttggttggt ggacgaacac
      481 tggacgctgc ccgcgagcga ggcttcgccg gaacagcatt gacggctccc gatgctacgg
      541 cccttgggac gaaaattgag cttcatttta gctctccccg ccggctcgtg tatctcgccg
      601 gacgcgatcg caatccggga cttgagaaca agctcacaga tttgggtcac gcaatcgagc
      661 tggttgaggt ctatgcggcc caaccggccg actccttgac cgaggacgct ttggcgctgg
      721 caggacaagg gaaagtcggc gcggttctgc actattcacg gcgcagcgcg gagatttacc
      781 ttcgtctagc ccgcaacgcc ggcctcgact tgtcgcgggt aaaccatgtc tgcatatcgc
      841 acaatgcggc agcgccgctg ctggccgccg gcatccatga agttcttgtt gccaaagctt
      901 caaacgagca ggcgatgttt ggtctcgtta acgccttggc tggcttgccg gaaacgccac
      961 tcggacaagc ggaaaaattg gactcttgat acgcaaaggc ttgatcaaac taggtattcg
     1021 aagcatcatc cccggatgat ccggcatatt ggtttgactg cgcttgtcgt cgcattgctt
     1081 tctctagctt tccctccggt gtttgcggcg gacattgtcg aagtcaaaac cggcttcatt
     1141 cgcttggccc atctgcagga gaccgtatcg attctcgact ctccggctgc agacgatggt
     1201 atagctgggg cgaagctcgc gatcgaagac aacaatacaa ccggccgttt tcttggccag
     1261 acatttgcca tcgaggatgt cgtccttaat cctgatggcg accctattgc cgcgcttgat
     1321 ggtctcgtaa agcgaggcat ttttctgctt atcgccgaac tcccggctgc cttgctgctc
     1381 acgctcagcg atgcagcaaa agacaagggc gtgcttattt tcaacattgg cgccccagag
     1441 gattcgcttc gggaggagaa ttgccgcgcc aacgtctttc acattgctcc ctcttattcc
     1501 atgcttgctg acggccttgc tcaatatctc gtctggaaac aatggaaacg ctggttcctg
     1561 atggagggct cgcatccgga agacacgctt ttcgccgggg cgctcgagcg ggcagcaaaa
     1621 aaattcggcg ccaaaatcgt ggaggagcga gtctatcagg ataccggcgg cgggcggcgc
     1681 tctgactcag gaagcgtgca gacccaaaag ctcattcccg tcgcaactca aaatgcgcca
     1741 gcctatgatg tccttctcgc ggcggacgaa agcgaggttt ttgccaacta cctcccttac
     1801 cgcacatggg atccgcgtct cgtcgccggc tcggctgggc tcaagccgat gagctgggat
     1861 ggaagccatg agcaatgggg cgcaattcaa ttgcaaaatc gtttcatgaa gcttgccttc
     1921 cggcgcatga atgcacgcga caacaatgcc tgggtagcga tgcgcatgat cggcgaggcg
     1981 gcgtcgcgtg ccggttcgaa cgaccccaaa atcattcgca gttatcttac cggaccggat
     2041 ttcgctatcg ccgccttcaa aggccaaaag cagacattgc gccgctggaa ccagcaacta
     2101 cgccagccta ttctgctcgg ggatgggcgg acgatcgttt cggtctcgcc gcaagagggc
     2161 tttttgcatc aaacttcgca gctcgatacg ctgggcttcg acgaaccgga aacgagatgc
     2221 catctcgagt aattttccaa gagggactaa aggcctgtgc gcttacccat gtcccccttc
     2281 ggcttgcctt tattgcgttg gcctgtgtca cggcattcca agcccaggcg tttacggctt
     2341 atgtttcaaa cgaaaaaggc aattccatct ccgtcatcga tacagacaaa atggaagtga
     2401 cggagacaat tttagtcggt catcggccac gcggaatagc ccttagccac gacggcggtc
     2461 agcttttcat ttgcgctggc gacgaagatt tgattcagat tctcgatacc aagaggcgct
     2521 cgatcgtcgg cactttgcca tcaggccccg atcccgagct cctggttctg agcccggatg
     2581 gtaagctcct ttatgttgcg aatgagaacg acaatatggt gaccgcgatc gatgtcgcga
     2641 cgcgcaagat cgccgccgaa atccccgtcg gagtcgaacc ggagggaatg gagctcagtc
     2701 ccgacggcaa agtgctggtc aatacgtccg agacgacgaa tatggcacat ttcatagata
     2761 cgcatacgca caagattttt gccaatgtcc tcgtcgatgc gcggccgcgg gtcgctcagt
     2821 tcacgccgga cggtttggaa ttatgggtct cctctgaagt cggcggcacc gttagcgtaa
     2881 tcgatgcggg caatcccaag gtgaagcaga agatcacatt tgatgttcct gggctttcaa
     2941 aggaagcgat ccagccagta ggcttgaggt ttacgcagga cggcaggacg gcattcgtag
     3001 cgcttggtcc ggcaaaccgg gtcgcagtga ttaatgccgc aacaaagagc atcgaaaaat
     3061 atcttcttgt cggtcagcga gtctggcaaa tggcatttac ccccgacagc aaatttcttt
     3121 atacgaccaa cggcgtttcg aacgacgttt cggtgatcga tgtggcgagt ctcaaggtcc
     3181 taaaatcaat cccggtcggt tcctttccct ggggcgtggc gatttcacgc caatgagttt
     3241 ttcgacgccg gagccggaag cagcaatcgg gcgcccttcg cgcttgaccc tggcgctcga
     3301 aattggttgt gtcagctaca gctacggcaa acgcaaggcc ttggaggatg tttctttcag
     3361 tgtcgccccg gggagtttta ctgtccttct cgggctcaat ggcgcgggca agagcacgct
     3421 ttattgcttg atcacccgcc tctacgcgac gcaacaaggc aggatccgta tatttgggtt
     3481 cgacgtgaac cgctcttcgg gagaggcctt gcggaggctc ggagtcgtgt ttcaagcgcg
     3541 gacgctggat cttgacctga cagtcctgca aaatctttcc tatcacgcgg cgctgcatgg
     3601 gatttccaca aaggaaacaa agcgtcgcgc gaatttggtg ttagaaatga ttgcattgac
     3661 tgagcgtaaa aatgataaag cgcggaacct gtccggcggc caaatgcgcc gggtcgaaat
     3721 cgcccgcgcc ttgctgcaca gaccgcagct gttgcttctc gacgagccaa ctgcaggtct
     3781 cgacgtcaaa gcccgagccg atattttgat gcatgtgcgc aaccttgccg cgcaggaggg
     3841 cattggcgtg ctttgggcga cgcatttgat cgacgaagtc gaggccgccg atgacgtcgt
     3901 gattttgcat cacggtaagg ttcttgactc tggcaaagcc tctgacattg ccggctgggc
     3961 ggtcgccact gaatttcgcg ccgcttttga caaaatgacc gctgcaagcg gaaaagcgca
     4021 gcaatgacgc cagatgtgat ggcaaggccg ggctaccggg gttacagcat tgtcaattat
     4081 gcccgctgct tcaaagggat tgtctggcgc gaaaccctgc gctactggca ccagcgcgag
     4141 cgatttttat cggccctggt gcgtccgctc gtctggcttt tcatttttgc ggctgggttt
     4201 cgttccgtgc tgggcgtttc gattattccg ccctacgaga cctatgtgct ctatgaagtt
     4261 tatat
//
LOCUS       DATMPU010000343         6838 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_2744739,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000343 DATMPU010000000
VERSION     DATMPU010000343.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 6838)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6838)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6838
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2744739"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            334..1008
                     /locus_tag="VNY06_07840"
     CDS             334..1008
                     /locus_tag="VNY06_07840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016920273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphotransferase family protein"
                     /protein_id="HWX14745.1"
                     /translation="MTVFALDSIDLAALLCSRICHDIISPVGAIINGLEVLEEEKDAE
                     MRSFALDLIKKSAEQASARLQFCRLAFGAAGSAGASIDTSDAEMVARGLFNRERTELR
                     WNAPSLIMPKNKVKLILNLCLIATSAMPRGGVIEVIVTGADDEVRIRVSAKGPNPKLA
                     NHVPLLLAGTLDYAPSTPQDIQAYYTGLVARAASMILTVAAHSENLTIEAESPRAEVT
                     VEPAAI"
     gene            complement(1165..1632)
                     /locus_tag="VNY06_07845"
                     /pseudo
     CDS             complement(1165..1632)
                     /locus_tag="VNY06_07845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010969554.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
     gene            complement(1643..2038)
                     /locus_tag="VNY06_07850"
     CDS             complement(1643..2038)
                     /locus_tag="VNY06_07850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012383933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3126 family protein"
                     /protein_id="HWX14746.1"
                     /translation="MDKAELRKLQEFLRKSLGNQGIKVTPGKIDSDDADVYLGERRIG
                     VITVDDEDGDRSFSFEMKIPVERPVLQDYLRRLFETDRLKIVPRGRKNDSVELVSGED
                     FVGVISADDPKAKSFTLQMAILDIDLEEF"
     gene            complement(2101..2934)
                     /gene="cysE"
                     /locus_tag="VNY06_07855"
     CDS             complement(2101..2934)
                     /gene="cysE"
                     /locus_tag="VNY06_07855"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012383932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HWX14747.1"
                     /translation="MVSSEGTAKVVALARTDPMFARLLAEAEETARREPDIAGVIFTA
                     VLNHDSLEAAIIHRLAGRLGRRALPGDLIEQTYNELIAADASIADAFRADIAAVVDRD
                     PACTRLIEPVLYFKGFHAIETHRLAHALWIAGRQDFALYLQSMSSEVFQTDIHPAARI
                     GKGIFLDHATGLVVGATATIGDNVSILQDVTLGGTGKERGDRHPKIKQGVLIGAGAKV
                     LGNIEIGRCSRIAAGSVVLHSVPDNKTVAGVPARIVGDAGCAEPSRVMDQLLAPASRT
                     E"
     gene            complement(3060..3749)
                     /locus_tag="VNY06_07860"
     CDS             complement(3060..3749)
                     /locus_tag="VNY06_07860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012589127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HWX14748.1"
                     /translation="MLLIHGFASTHAVNWIFPLWVKTLTEAGRRVIAFDNRGHGRSEK
                     FYDPAAYSLSEMAEDARALLDALDIAVVDVMGYSLGARIAAFLAKAHGHRLRRMILGG
                     LGDHLVIRAGLPLSIAAAMEAPSVESLVDPTDRSFRSFAEATKSDLKALAACVRGARQ
                     ALTEAEVREINIPILIVMGTEDHVGGDPHALAVLFKRARTVHIPGRDHNRTVGDKVYK
                     ASVLDFLDESP"
     gene            3964..4188
                     /locus_tag="VNY06_07865"
     CDS             3964..4188
                     /locus_tag="VNY06_07865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020177025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2093 domain-containing protein"
                     /protein_id="HWX14749.1"
                     /translation="MNRYERRPISVDEAELEYLNNEFRLLRPGGFVRCAVTGVAIKLE
                     DLRYWSVDFQEPYAGPEAKLQRLGITPESN"
     gene            complement(4267..4644)
                     /gene="rplT"
                     /locus_tag="VNY06_07870"
     CDS             complement(4267..4644)
                     /gene="rplT"
                     /locus_tag="VNY06_07870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014890448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HWX14750.1"
                     /translation="MARVKRGVTSHAKHKKILKTAKGFYGRRKNTIRAAKGAVDRSMQ
                     YATRDRRTKKRDFRTLWILRLNAAAREHGLTYSRLIGGLAKAGVEIDRKMLAELAISE
                     PQAFRAVAEKAKSALSALARASG"
     gene            complement(4697..4897)
                     /gene="rpmI"
                     /locus_tag="VNY06_07875"
     CDS             complement(4697..4897)
                     /gene="rpmI"
                     /locus_tag="VNY06_07875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012335024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HWX14751.1"
                     /translation="MPKLKTKSGAKKRFKITATGKVLYAQRGKRHGMIKRTNKQIRNL
                     RGTAVMFKSDGDNVIKYFLPND"
     gene            complement(5090..5812)
                     /gene="pgl"
                     /locus_tag="VNY06_07880"
     CDS             complement(5090..5812)
                     /gene="pgl"
                     /locus_tag="VNY06_07880"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012590809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HWX14752.1"
                     /translation="MKPAPIVEVTSDTEALALNAARFIAARFSAASTRFSVSLSGGAT
                     PKRVYELLAVTNFTPAIDWQKIHLFWGDERFVPPDHRDSNFRMAQEALLDRVPIPGTQ
                     VHPIPTSYDSPERAAISYETSLQEFYQAKEFDPARPLFDLTLLGLGEDGHTASLFPNT
                     KALEERNAWVTSTIGAKPEPRISLTYPALESSREILFIVSGAKKQQILSRVLANDPTL
                     PAARLETGGIIRIFADQEAMGN"
     gene            complement(5842..6062)
                     /locus_tag="VNY06_07885"
                     /pseudo
     CDS             complement(5842..6062)
                     /locus_tag="VNY06_07885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013168628.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconate dehydrogenase
                     (decarboxylating)"
     gene            6563..6838
                     /locus_tag="VNY06_07890"
                     /pseudo
     CDS             6563..6838
                     /locus_tag="VNY06_07890"
                     /inference="COORDINATES: protein motif:HMM:NF028290.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
BASE COUNT         1523 a         1971 c         1847 g         1497 t
ORIGIN      
        1 ctccatcacc gcgacacctg catcgagcgc gatctcagcg aaaatttctg ccacggcatc
       61 ggcgtcgaac gcctttactt tgcaagtaac tatgtctttc ttggttttat acatgaggtt
      121 ttctgaaaca gacgtttgtc acatgttgcg agatacgata atttgttaac ctctgattcg
      181 cagactagat agatctttga cgtctcgtgc cacggattcg actcggcttg ccgttacgta
      241 ttggtacctt acgcgcgctg gcgacagctt cgggcattgc gcgcgatgca actttgtagc
      301 gcgatgcata gacgcagagc gaagggatgg ttcatgacag tgtttgccct cgactccatc
      361 gatctcgccg cgcttttatg ctctcgtatc tgccatgaca tcatcagccc cgttggcgcc
      421 attatcaatg ggcttgaggt gctcgaagag gagaaagatg cggaaatgcg cagctttgcc
      481 ctcgatctca tcaaaaaaag cgcagagcag gcttcggccc gtttgcagtt ttgccgcctc
      541 gcattcggag ccgcgggctc ggccggggct tcaattgata ccagcgatgc ggaaatggtt
      601 gcacgtggcc tttttaaccg cgagcggacg gaacttagat ggaatgcgcc ctccctcata
      661 atgccaaaaa ataaagtcaa gctcattctc aacttatgcc tcatcgctac ctcggcaatg
      721 ccgcgcggag gcgtgattga agtcatagtg actggagccg acgatgaggt gcggatcaga
      781 gtatcggcca agggtccaaa tcctaagctc gcgaatcacg tgccgctcct ccttgccgga
      841 actctagact atgcgccctc taccccgcaa gatatccagg cctattacac tggcctcgtt
      901 gcaagggccg caagtatgat tctgactgtg gcggcgcata gcgagaatct caccatcgaa
      961 gccgagtcgc cccgagcaga agtgacggtc gagcccgccg caatctaggc catgctcttt
     1021 ccagcttctc ttcacaccga ccttgtgggg atggttcaag caaaaaacga tcttgtgagg
     1081 tccattaggc tcgtcttgtg ggatttccgc ggcgctcagc cgatcgcccc aaggccttcg
     1141 gcaaaacgcc gacccattgg cggcataatg cgcggcgctc tcacggagct tctgagccga
     1201 cgctacatcg agcttgcgcg cgagccggcc ggaaccccaa tgatcaggct atcgtccgga
     1261 aactctttcc cttccgtcac caaggcgttg gcgccgacaa cgcaactgcg accgatctta
     1321 gcaccatcca tacgatgagg ctgttttcgc cgatcgtgca gccatgtaga attgcgtgat
     1381 ggccaatggt gcaacccgga ccaattgtca agggaaagcc taagtcggtg tgaagcagtg
     1441 agccatcctg tatattagag cgggcatcga cgaaaatatc ctcgtcatcg ccttgcagaa
     1501 gcgcgccgaa cctgatcgag acatcggcct caagccgtac tttgctgatg acatgagcac
     1561 cgggcgcaat ccagaactgg ccatccgctg ggggactatg ggtgaatcgc cttcaagcgc
     1621 gtagagcggc attccgcccc tcctaaaatt cctcgagatc gatgtcgagg atcgccatct
     1681 gcaacgtgaa gctcttggcc tttggatcgt cggccgaaat gacgccgaca aaatcctcgc
     1741 cactcaccaa ttcgaccgaa tcattttttc ggccgcgcgg cacaatcttc aatctgtccg
     1801 tctcaaaaag ccgccgcaaa taatcctgca gcactggtct ctcgacggga attttcatct
     1861 cgaaagaaaa agacctgtcg ccgtcctcat catcaaccgt gattacgccg atccggcgct
     1921 caccaagata cacatcggca tcgtccgaat cgattttgcc aggcgtaact ttgatcccct
     1981 gattgccgag agatttgcgc aggaattcct gcaatttccg caattcagct ttgtccacga
     2041 tgcgctccgc agaggccagg tcaggtccca aaccgggcag ctgacactcc atgcgagaaa
     2101 ttactcggtg cgtgaggctg gagcaagaag ctggtccatg acgcgcgagg gctcggcgca
     2161 acccgcgtcg ccgacaattc tcgcgggcac acccgcaacc gtcttgttat ctggaacaga
     2221 atgcagcacg accgaaccgg cggcaatgcg tgagcagcgg ccaatctcga tattgccaag
     2281 aacttttgct ccggcgccaa tcagcacgcc ctgcttaatt ttcggatggc gatcgccacg
     2341 ctccttgccc gtgcctccca gggtcacatc ctgcaggatc gagacattgt caccaatagt
     2401 ggcggttgcg ccgacgacga ggccagtcgc atggtcaaga aaaatgcctt tgccgattcg
     2461 agccgccgga tgaatgtctg tttgaaacac ctcggacgac atgctctgga gataaagcgc
     2521 aaaatcctgg cggcctgcga tccaaagtgc atgcgctagc cggtgtgtct caatcgcgtg
     2581 aaaccccttg aaatagagga caggctcaat aagccgcgtg caggcaggat cacgatcgac
     2641 caccgcggca atatctgccc gaaaagcatc ggcgatcgaa gcgtcggcgg caatcagttc
     2701 gttgtaggtc tgctcgatga gatcgcccgg taaggcccta cgtccaagtc ttcccgccag
     2761 gcgatgtatg atcgccgctt caagcgaatc gtgatttagg accgctgtaa aaatgacacc
     2821 cgcgatatct ggttcccttc gtgcagtctc ttccgcctcg gccagcaaac gcgcaaacat
     2881 gggatccgtg cgggcaagcg caacgacttt cgctgttcct tcgcttgaaa ccatgtcacg
     2941 ctctccgcat ctgtccagcc tggccccaag ccggtttgaa ccccgtttta ctgcgggagc
     3001 acccgcgagc ggtgaactgg cacgccgtca cagaatatag ttggctgaga ccccaggggt
     3061 taggggcttt cgtcgagaaa atccaatacg ctagctttat agactttatc gcctaccgtt
     3121 cgattatgat cgcgaccggg aatgtgcacg gtacgagccc gcttgaatag gacggcaaga
     3181 gcatgcggat cgccccctac gtggtcttcc gtgcccatga caatcaatat aggaatattt
     3241 atctcgcgga cttccgcttc ggtcagagct tgccgggcgc cccgaacgca agcggcaagc
     3301 gccttaaggt cgcttttggt tgcttccgcg aagctacgga aggaacggtc cgtcgggtcc
     3361 acgagacttt cgacggaagg cgcctccatc gccgcggcaa tgctgagggg gagtcctgcc
     3421 cgaataacaa gatggtcacc aagccctcct aggatcattc tgcgcaggcg atgaccgtgt
     3481 gctttggcca aaaatgcggc aattcgcgcg cccagcgaat agcccatgac atccacaacc
     3541 gcaatatcga gagcgtcgag aagcgcgcgg gcatcttccg ccatttccga caaagaataa
     3601 gcggcgggat cgtagaattt ttcgctgcgg ccatggccgc ggttgtcgaa agcgatgacc
     3661 cgccgcccgg cctcggtcag ggtcttaacc cacagcggaa agatccaatt gaccgcgtgg
     3721 gtcgaggcaa agccgtgtat gaggagcacg ggcttccctc ggctgcaagt tgcgggcatc
     3781 tcgtcgatga aggctaggcg gacaccacgg tccaaaattt cgtacatgcc cgaccctttt
     3841 gcgtaaccct ttggcctcca cttccatcaa aataacttcg cgatcgtttg ccggcatagt
     3901 agaaaatttg gcggggctgc aatatattga caccaaatct attcttatag agtacaggat
     3961 tagatgaacc ggtacgaacg tcgtcctatc tctgtggacg aagccgagct tgaatatctt
     4021 aataacgaat ttcgtcttct gcgcccagga ggctttgtgc gctgtgcagt cactggcgtg
     4081 gcaatcaagc ttgaagatct gcgctattgg agcgtcgatt ttcaagagcc ctatgccggc
     4141 ccagaagcca aactgcagag gctcgggatc acgccagagt caaattaaac gcgaccgcga
     4201 tccggtctag ctgccgagga cgatagcccg acctgtatgg cacggcacaa tttcgttgcg
     4261 cggcggttaa ccgctggctc gagctagcgc cgagagagca ctcttcgctt tttcggcgac
     4321 cgcccgaaat gcttggggtt cgctgattgc gagctccgcg agcattttgc ggtcaatttc
     4381 gacaccagcc ttggcaaggc ccccgattaa gcgcgaatag gtgaggccat gctcgcgcgc
     4441 cgccgcattc aggcgcaaaa tccataaagt gcgaaaatcc ctctttttcg tgcggcggtc
     4501 gcgcgtcgca tattgcattg agcggtcgac cgcccctttc gcggcgcgga tcgtgttctt
     4561 gcggcgcccg taaaatcctt tggcggtctt caaaatcttc ttgtgctttg cgtgcgaggt
     4621 gacgccgcgc ttaacgcgag ccatggtctg tctccagata agtcaaagaa cacgccaaac
     4681 gaaacagtgg gaccatttag tcgtttggca aaaaatattt aatgacgttg tcaccatcac
     4741 tcttgaacat gaccgcggtg ccgcgcagat tccggatttg cttattggtc cgtttgatca
     4801 tcccgtggcg cttgcccctt tgggcataga gcactttgcc tgtcgcggtg atcttgaatc
     4861 gctttttggc accagatttg gttttaagtt tgggcatttg cttatgtctc cgcgggggca
     4921 aacgcccaag aatacatgct taccgagatc ccgcccctac ggctcaaagc gaaagcgctc
     4981 gcagcgcacg aaaaccgctg tggcattcct attagccggg cggttcgaac ccgcgcttat
     5041 aagcaatgca acaaaaaaag caagtcatgc ccggcagcaa ggcatcagat tagttcccca
     5101 tcgcctcttg atcggcaaag atgcggatga tccctcccgt ctcgaggcgg gctgcgggta
     5161 gcgttgggtc attcgcaaga acgcgagaga gaatctgctg tttcttcgcg ccagagacaa
     5221 tgaagaggat ttccctactc gactcaagtg ccggataggt cagcgagata cggggttccg
     5281 gctttgcccc aatggtcgat gttacccatg cgttgcgctc ttcgagcgcc ttggtgtttg
     5341 gaaacagcga ggctgtgtga ccgtcctccc caagaccaag caaggttagg tcaaacaatg
     5401 gccgtgctgg atcgaactcc ttcgcctggt aaaactcttg caggcttgtt tcgtaggaaa
     5461 ttgcggctct ctccggcgaa tcgtaacttg ttgggatcgg gtggacttgc gtaccaggaa
     5521 taggcacgcg atcgagcaag gcttcctgcg ccatgcgaaa attgctgtct cggtgatccg
     5581 gcggaacaaa gcgttcgtcg ccccaaaaca aatgaatctt ttgccaatca attgcgggag
     5641 tgaagttggt tactgcgagg agttcataga cccgcttcgg agttgcgccg ccggaaagac
     5701 tgacactgaa ccgtgtggac gcggcggaga agcgcgcagc tataaagcgc gcagcattca
     5761 gtgccagcgc ttccgtatca ctcgtaactt cgacaattgg cgccggtttc attcgaactc
     5821 cgatttgggc ggtcgcatgt cttacttgcc gggcgaatga gcatttttgc gttcgacatg
     5881 accgccaaat ttgaagcgca ttgccgatag gagcatgtca ccgaaattat gctcctgccg
     5941 ggaacgaaag cattcgaaaa gagaagctgc gagaacattg gcgggcaccg cctcctcaac
     6001 ggccgcatcg aattccaacg gccctcgcct gaatcctcga cgtcaccggt gaaatttgca
     6061 agcgccgggc tccgcaagca ccatggcagt caggccgatt tcggcgaagc cataaggcgt
     6121 gatccaacat cggcctcatt gcgcctcgat cgcgccgcga cctttgaggc caagggcgat
     6181 cttggatcag gccattgcca gttacgatga ggtgatcaaa cgcgatcaaa aagctcgcaa
     6241 ccgcctataa tcgacgcgga cttgcttttc cgctaaaggc gatatcgacc aagcgcttgc
     6301 cgatttcagc gaggcgttgg cgctcgatcc cacaattgcg gacgtactcc tcaaccgggc
     6361 gcaagcctat cgtggaaaac atgatctcga ccgcgctcgg caagatctcc aagccgcgtt
     6421 ggagattaac ccgcagctca cctaagcgaa agatgcgctc gatgggtgaa caagcttgtc
     6481 gccgagagcg ccgcgcctcc aacctctgca tccaatcatg taaacttacc ttggccgttc
     6541 gctctgcctt cgcagggagc cacttgtggt cctggaatgg tggcaaatcc actatgagaa
     6601 agcagctttt tgggcggggc tgaccctaat tggtctcagc gcggtcgctg gcccatccgc
     6661 ggcggcggca gactttgtcc acagcatggc actcgaatat ttgcctttca tactgatgct
     6721 ttttgcgctt tacactgcag ccggaggcat ttcagtcgag ggcaagctca aaggtacgcc
     6781 ggtcatcaac accacgatcc tcggtctcgg cacactaacc gtatcatcgg acaactgg
//
LOCUS       DATMPU010000344         2623 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_3688425,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000344 DATMPU010000000
VERSION     DATMPU010000344.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 2623)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2623)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2623
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3688425"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            193..504
                     /locus_tag="VNY06_07895"
     CDS             193..504
                     /locus_tag="VNY06_07895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX14753.1"
                     /translation="MALIAFEHPLSEVQMRAVIALGWKSEKAAERTLVDCALRQPRQY
                     IPHDKRETVRRLICGKFSAAFHNASVTDNKNVLAGQSLSVTCQLRDDRRHDRLAPRDL
                     G"
     gene            complement(1264..2589)
                     /locus_tag="VNY06_07900"
     CDS             complement(1264..2589)
                     /locus_tag="VNY06_07900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012590387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX14754.1"
                     /translation="MRPLECEDIGGKAVFRTKRLAESQSIPLRSSSGFGFVLLARALW
                     EQEGQAAFMSTSAREIDIFPADGLRNFLTFCRLPRLLYSSQEGFAPPLDADRWELFAR
                     RINPHFKRVQSQFWLARKNGKLAGRIFAQVYDETSAPRGASRAQFGCLDAIGDDAVVA
                     GLTRAAEEWLRQRGALQVHGPFSPSINSESGLLVEGFHEIPMIAVPWHPSYLGPSLER
                     QGYAKARDLVSYRRDVGEKDRAAEPGIAARHDWRKRFNIRAIGIKNLKQEAKTIVDIF
                     NDSWSENWGFVPFTLDEFLSSIGALKLVAPSDSGFMKGGFMIEVDGMPQAFALVLPNL
                     HEIMADLGGRLFPFGVLRLISRIRSHGYRSGRLALFGIRRALHGKIAGGVIILAFIAE
                     LTRRSVLSPIEQVEFGWVLEDNQGMRRPIELSGAHVYKVHRIYEKNLAA"
BASE COUNT          549 a          800 c          726 g          548 t
ORIGIN      
        1 cgctggaccg ctatgcaggg cacgctttgc aaatgaagag ttcgcgtcgt ccgatggcaa
       61 atcgcgctcg cgcatagtgc aggcgcattt tccggatgat tgcgtgagac ccacgcggcc
      121 cctcacacac gttcgccgtc caaaacctct tgaagctccc ttgagatagt gactggcctg
      181 gcatcgcctg caatggctct catcgccttt gagcatcccc tctccgaggt gcaaatgcgc
      241 gcggtcatcg cccttgggtg gaaaagcgag aaagctgccg agagaacgct ggtcgactgc
      301 gcgctgcgcc agccgcgtca gtatatcccc cacgacaagc gagagacggt gcgccggctt
      361 atttgcggca agttctcggc ggcttttcac aacgcatccg ttaccgataa taaaaacgtt
      421 ttggcggggc aatcattatc ggtaacatgc cagctccgtg atgatcgccg tcatgatcgc
      481 ctggcaccgc gcgacctcgg ctagctcggc ttgaagttga cgcacgaagg cctcggcacg
      541 tgcgatggta caattttgtg ccgccacggc cgcctgctca cgggcttgct tgcgtcttcg
      601 gtagtatgtg ctgcgcgaga cgcccatggc gagccacggc ttacttgccg accgtcgcgg
      661 gcctattccg tgtgcccggc gccagcgctc cgaacgaagg cggttcgcct cgcggcgctc
      721 ggctgttgtc attgcttgcg gaactcgcgc aaaaactcgc gcaggaactt tgcttcgcgc
      781 tcttcccatt tcaacccgta tttaagccta tgaccatcgg ccagctcggc cagccatcgc
      841 tcatctgtgt agcctttcgg ccggatcgcc gccgtccgtt gaaggatggt ttggcgcgcc
      901 tcgtcaagac ttttaaggcg gcaccggcat catctacttt cagttgccgc aaggggcgct
      961 cctagccatt aagcccccct ttaattggcc actggcgccc gccttaaccc ccctttggcg
     1021 ggcgcttttt tgccggttgt cgattgacac gctggctgcc ctaggtggta agctcgcggt
     1081 gaaaaacaac tagagccgtt atcgcgagca cgattattta cggcataaca gacatgagga
     1141 aatcccccga ttagccaagc gacgaggttg aacagtaggc cactcatgcc ttgtgccgaa
     1201 ccccgccgtc ttgtgcttca ctcccatgcg gacattgctc gtcctgcagg agggaggggt
     1261 atgtcacgcg gcgagatttt tttcgtagat gcgatgcact ttgtagacat gcgcccctga
     1321 gagttcaata ggccgccgca tcccttggtt gtcctccaaa acccagccaa attcaacttg
     1381 ctcgatcggg ctcaaaacac ttcgccgggt caattcggcg atgaaggcca aaatgatcac
     1441 gccgccagcg atcttgccgt gcagggcgcg cctgatgcca aagagggcaa gccgccccga
     1501 cctgtatccg tggctgcgga tccgtgaaat caagcgcagc acgccaaaag gaaacagacg
     1561 acctccgagg tcagccatta tttcatgcaa gttcggaaga acaagagcga aagcctgcgg
     1621 cataccgtcg acttcgatca tgaacccccc tttcataaac ccgctatcag agggtgcgac
     1681 caatttgagc gccccgatac tcgacaaaaa ctcgtcgagc gtaaacggca caaagcccca
     1741 attttcactc caggagtcat tgaatatatc gacgatggtc tttgcttcct gtttgaggtt
     1801 tttgataccg atggctctga tattgaacct tttgcgccag tcatgccgcg cagctatgcc
     1861 tggctcagcg gcgcggtcct tctcgccaac atcgcggcgg taggacacga gatctcgcgc
     1921 cttcgcataa ccctgccgtt ccaaagacgg gcctaaataa gatgggtgcc agggcacagc
     1981 gatcattggg atctcgtgaa atccctcgac aagcaggccc gactcgctat tgatcgaggg
     2041 tgagaatggc ccatgcacct gcaaagcgcc gcgctgccgc agccactctt ccgcggcacg
     2101 cgttagaccg gcgacaaccg catcatcgcc gatggcgtcg aggcacccga attgcgcccg
     2161 cgatgcgccg cgcggtgcgg aagtctcgtc gtaaacctgg gcgaaaatcc ggccagcgag
     2221 cttgccattc ttgcgggcga gccagaactg cgactggacg cgtttgaaat gcggattgat
     2281 cctgcgcgcg aataattccc accggtcggc gtcgagaggc ggcgcgaaac cctcttggct
     2341 gctatagaga agccgcggca ggcgacaaaa ggtgagaaaa ttccgcagcc cgtcggccgg
     2401 aaaaatatca atctcgcgtg cgctggtgct catgaacgct gcttgacctt cttgctccca
     2461 taaggcacgc gcaagcaaaa caaatccaaa cccgctggag cttcggagcg ggattgactg
     2521 gctttccgca agtctcttgg tgcggaaaac agcttttcca ccgatatctt cacattcgag
     2581 ggggcgcatc gcgaagaggt acaggacttg caccttgtaa tct
//
LOCUS       DATMPU010000345         3380 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_2422938,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000345 DATMPU010000000
VERSION     DATMPU010000345.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 3380)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3380)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3380
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2422938"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1..746
                     /locus_tag="VNY06_07905"
                     /pseudo
     CDS             1..746
                     /locus_tag="VNY06_07905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012631102.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
     gene            complement(827..1810)
                     /gene="hisG"
                     /locus_tag="VNY06_07910"
     CDS             complement(827..1810)
                     /gene="hisG"
                     /locus_tag="VNY06_07910"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018408894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HWX14755.1"
                     /translation="MSLREKALIVAVPSKGRLQENAAAFFARAGLDLVHGSRGYRGTL
                     AGIENVEIAFLTAADIVAALAAGDVHLGVTGEDLIRETIAAPEQKVALLAPLGFGHAD
                     VVVAVPRAWIDVKTMADLEDVAAAFRARRGERMRVATKYVQLTRRFFAERHVTDYRIV
                     ESLGATESAPAAGKAELIVDITTTGATLAANALKILDDGTILRSQANLVASLGADWNL
                     GAREKARLILSRIAAEEEARTTREVSAVLSRFDDSTLAGAKEVFSARLRFYAADGRLA
                     LNCPKDRAAPLADWLIAQGASYVCVGAQDYVFLAENPLYEKLIVRLPVAAD"
     gene            complement(1807..2916)
                     /locus_tag="VNY06_07915"
     CDS             complement(1807..2916)
                     /locus_tag="VNY06_07915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018408893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HWX14756.1"
                     /translation="MSVADQSAARIIAHLVGAGYVRAEPPILQPASIFFDSGEDLRAQ
                     LYLTSDLSGADYCLRPEYTIPVLAAYLAGPTAGERAAYSYCGPVFRYSPGAPSEFIQA
                     GIENFGRVDREAADAEILALALNATAEARKIEPTVRIGDAGLFSKFLEALDLSAEWRR
                     RISRGHAQGKSLGMILRPLPNGSSADHSGVLALIERADRQGARALVKDLLSIAGVAPA
                     GGRTPGEIADRFLEQAALRSSAGFCIEKRAVIEQFLAIEGSPQDASKRLRALAAEARL
                     DLSAALDRFDKRLEHIAAQGLDVAKIDFAARFTRNLDYYTGFVFEAKEQGAPEPVAGG
                     GRYDQLPQTLGAKQPIPAVGAAIWLDRLGAKEPCP"
BASE COUNT          720 a          998 c         1028 g          634 t
ORIGIN      
        1 tatctgccca aggtctcgga gatgacggat ttcaacatgt ggacgtggaa cagccgggtt
       61 tttcccggca tcgatccgct gccagtgcgc ctcggcgacc gtgtgcatgt gcgcatggcc
      121 aatttgagca tgaccaatca tccgatccat ctgcatggtc accacttctc ggtcgcgggc
      181 accgatggcg gctgggtgcc ggaatccgcg cggtggcctg aggccacgat cgatgttccg
      241 gtcggcgtga tccgcgcgat cgaattcatc gccgataatc cgggagactg gccattccat
      301 tgccacaaga cgcatcacgc tatgaacgcc atgggtcaca acgtgagaaa tttcatcggg
      361 gccgcgcaga agcgcagtct cgtcgatgcc gtccgcaagc tggcgccagg gtatatgccg
      421 atgggctcgt ccggcatggc ggacatggat aagatggcga tgccggcgcc cggcaacacg
      481 ctgccgatga tgaccggttc cggccagttt ggcccgatcg agatgggcgg catgtttacc
      541 gtcatcaaga tccgcgaagg cctcgccgcg gatgattaca aggaccccgg cccgtataat
      601 acccggaagg caccgtcgcc tacgagttca agggccaggt tggcgagcct ccacagcaga
      661 agagccaagc ggaaatgaaa atgccaggaa tggaaatgca gagcggcaaa cctagggtca
      721 agacaggccg caaggctcat cactaaatgc aagcaaggct cattatgact aggcgaagca
      781 cttctgtctg cttcgcggac cgctccgctc acgggtttgc ttccgctcag tctgcggcaa
      841 ctggcaagcg cacgataagc ttttcgtaga gcgggttttc ggccaaaaac acatagtctt
      901 gcgccccgac gcaaacataa gaggcccctt gcgcgatcag ccaatcggca agtggcgccg
      961 ctcgatcctt tgggcaattt aaggccaggc ggccatcagc agcataaaag cgcaaacgcg
     1021 ccgaaaagac ttccttcgct cccgcgagag tcgagtcgtc gaagcgcgag agaacagcgg
     1081 acacttcgcg cgtcgtgcgc gcctcttcct cggcggcgat gcgtgagaga atcaagcgcg
     1141 ctttctcgcg cgccccaaga ttccaatcgg cacctaacga agcgacaaga ttggcctgcg
     1201 agcgcaaaat cgtgccgtca tccaaaattt tcagcgcatt ggctgcaagt gtcgcgcctg
     1261 ttgtcgtgat atcgacgatg agctcggcct tgcctgcggc gggcgcgctc tcggtcgcgc
     1321 caaggctttc cacaatacgg taatcggtga catgccgctc ggcaaaaaag cgccgggtca
     1381 gctggacata ctttgttgcg acccgcatgc gctcgccgcg ccgcgcgcgg aaggccgccg
     1441 cgacatcttc cagatccgcc atagttttta cgtcgatcca ggctcgtggc acggcgacga
     1501 cgacatcggc atgaccaaag ccgagcggcg ccaaaagcgc gaccttttgt tctggcgcgg
     1561 caatggtctc gcgaatgagg tcttcgcccg tgacgccaag atgcacgtcg cccgcggcca
     1621 aggcggcgac gatatcggcg gcggttagaa aagcgatctc gacattctca attcccgcca
     1681 aagtgccgcg atagccgcgc gagccatgca cgagatcgag ccccgcacgg gcaaaaaatg
     1741 cagcggcgtt ttcttgcagt cgcccttttg aaggaaccgc gacgatgagt gctttttcgc
     1801 gcaagctcat gggcacggtt ccttagcgcc caaacggtcg agccaaatcg ccgcgccaac
     1861 ggcggggata ggttgttttg cgccaagcgt ctgcggcagc tggtcgtagc gaccgcctcc
     1921 ggcgacaggc tccggcgcgc cctgctcttt cgcctcaaac acaaagcccg tgtaatagtc
     1981 gagattgcgg gtgaaccgtg cggcaaaatc aatttttgca acgtcgagcc cttgcgcggc
     2041 gatatgctca agccgcttgt cgaagcggtc gagcgcggcg gaaagatcga gccttgcttc
     2101 ggctgccagc gcgcgcaacc gtttggaggc atcttgaggg ctgccttcaa tcgctaaaaa
     2161 ttgctcgatg accgcgcgct tctcaatgca aaatcccgcg cttgagcgaa gcgccgcttg
     2221 ttcgagaaag cgatcggcga tctcgccagg ggtacgcccg ccggccgggg ccacgcccgc
     2281 gatggagagc agatccttga cgagggcgcg cgcgccctgc ctgtcggcgc gctcaatcag
     2341 cgcgagcacg ccggaatgat cggcgctgct gccatttggc agcgggcgca aaatcatgcc
     2401 gagcgatttg ccttgggcat ggccgcgcga gatgcgccgg cgccattcgg ccgaaaggtc
     2461 cagcgcttcc aaaaatttgg agaaaagccc ggcgtcgccg atccggacag tgggttcaat
     2521 tttccgcgcc tcggccgtgg cgttgagcgc gagggcaagg atttcggcat ccgcggcctc
     2581 ccggtcgacg cggccaaaat tttcgatgcc cgcctggatg aattcgcttg gggctccggg
     2641 agaatagcgg aagactggcc cgcaataaga gtaggcagcg cgctcgcccg ccgtggggcc
     2701 ggcgagatag gcggcgagca ccggaatcgt atattccggg cgcaggcaat aatcagcgcc
     2761 ggaaagatcg ctcgtgagat aaagctgggc gcgcaaatcc tcgccggaat caaaaaagat
     2821 cgaagccggt tgcaggatcg gcggctcggc gcgcacgtag cccgccccga caagatgagc
     2881 gatgatgcgc gcggcgcttt ggtcggcgac agacaagtgt ttggcccgga gtgctaagaa
     2941 tttgcgcccc tcttagcaag gaagaccatg ttttgcgaca aattttggca ggcaaggatt
     3001 tgtccgattc cgggccagaa ccttaccaaa atgtttcacg tgaaacattt ttgtccggtc
     3061 ggggccgaaa accttacaag gccacttacg tgggggactt gtggcgtgtg gagttgcgca
     3121 aaaaagatgt cctattggca ccgggggtgc gccgcgaaag aagttcgctt gaccttagcc
     3181 tctttctccg ctggtccccg ggcaaattca aaattccgct ttacggcaaa ccgattttca
     3241 ccagaatgcc acccgcaaca tctattgact tctgcgggag tttattagtc ctggcaagga
     3301 aaccgttctt cgcttttaag cgccgcgttg ggctcgggac ataccgcatg aagaaccata
     3361 tgaagaaatg aagaagatgt
//
LOCUS       DATMPU010000346         9495 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_3688780,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000346 DATMPU010000000
VERSION     DATMPU010000346.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 9495)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9495)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9495
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3688780"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..206)
                     /locus_tag="VNY06_07920"
     CDS             complement(<1..206)
                     /locus_tag="VNY06_07920"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016917942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0030259 - lipid glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HWX14757.1"
                     /translation="MSGRCRIVVAAGGTGGHLFPAAAVSHALIARGFEIHLVTDERAA
                     KYGGDFPAQDVHRVASASPSGGSV"
     gene            complement(203..1348)
                     /locus_tag="VNY06_07925"
     CDS             complement(203..1348)
                     /locus_tag="VNY06_07925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012383699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative peptidoglycan glycosyltransferase FtsW"
                     /protein_id="HWX14758.1"
                     /translation="MVSRAERSHLANWWWTIDRWILGAVGALMMLGLVLTMAGSPPVA
                     ERLNLSTFHFVHRHVLYLVPSVTVLLATSFLSPRQVRRVALFVFLVSIALIIVALFFG
                     QEVKGSRRWIFGIQPSEFLKPAFVILVAWAFSEGAKRRDVPAVLLAVLLLLMTITPLV
                     LQPDFGQTMLVSLVWAALFFMAGLHWFWVAGIGGLGLGGALLAYKLVPHVRARVLKFV
                     DPGAAGGGIADTFQVDTALDCFLSGGWFGKGPGEGTVKRILPDAHTDFIFAITGEEFG
                     VLACLLIVAIFAFIILRGLLAARRNEDPFCRFAAAGLVLLFGIQSAINMAVNVHLLPA
                     KGMTLPFISYGGSSLISLALAIGFLIAVLRKRPGSAILAQAGAEVLA"
     gene            complement(1358..2761)
                     /gene="murD"
                     /locus_tag="VNY06_07930"
     CDS             complement(1358..2761)
                     /gene="murD"
                     /locus_tag="VNY06_07930"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012383698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HWX14759.1"
                     /translation="MTPVTCFDGKHAAVFGLGGSGFVAAQALVAGGANVTVWDDNEHA
                     REKARAAGLRIENLALADWTSFASLVLSPGVPLTHPAPHWTVQMASKAGVEIIGDIEL
                     FCRERRKIAPASPLIAITGTNGKSTTTALAAHLFQSFEFKVEVGGNIGTPILALAPPS
                     KDRVHVVEISSFQIDLAPSLDPAVGVLLNVTPDHLDRHGTMESYAAIKARLLEKADLA
                     VTGMDDAITAAIATERMKTGRKTTCISMTKTAIKYCNDYLFLDGTCLMRQSAGKLAMV
                     ADLAGIAALRGRHNGQNAAAAVAALGPLGLDCGQVEKGLRSFPGLAHRLEEVGRKGKV
                     LFINDSKATNANAAEKALLSFEKVFWIVGGRAKAGGIEPLRALFGKVVKAYLIGEATE
                     LFAKTIGDAFPFERCGTLDTAVLAAARDAQLSDLEEPVVLLSPASASFDQFADFEKRG
                     DWFRTIVKALLTGETEG"
     gene            complement(2764..3846)
                     /gene="mraY"
                     /locus_tag="VNY06_07935"
     CDS             complement(2764..3846)
                     /gene="mraY"
                     /locus_tag="VNY06_07935"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015931917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HWX14760.1"
                     /translation="MLTWLSEYASVFQPLNLFRYITFRTGGATATALFFVFFFGPGII
                     AALRLKQGKGQPIRDDGPKSHLLTKAGTPTMGGLMILSGVIVSTLLWANLRNHFVWIV
                     LLVTAGFGVIGFYDDLLKVTKQTHKGFSGRRRLAIEVGIAGIACLAMMKLGTPHVTGL
                     ALPAINGFAVDLGVFFLAFGAFVIVAAGNAVNLTDGLDGLAIVPVMIAAATFGIIAYL
                     AGNAIFSSYLGINFVPGAGELAIICGALIGAGLGFLWFNAPPAQIFMGDTGSLALGGA
                     LGTIAVAVKHEIVLAIVGGLFVLETLSVIVQVASFKLTGKRVFKMAPIHHHFEQLGWS
                     EPQVVVRFWIIAFVLALIGLSTLKLR"
     gene            complement(3857..5299)
                     /locus_tag="VNY06_07940"
     CDS             complement(3857..5299)
                     /locus_tag="VNY06_07940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012592439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoylalanyl-D-glutamyl-2,
                     6-diaminopimelate--D-alanyl-D-alanine ligase"
                     /protein_id="HWX14761.1"
                     /translation="MSEGTASDVLWTSLGLVGSLSARVSGSIPRGGVTGISIDTRSLQ
                     EGELFFAIKGPNSDGHDYVSAAFDKGAVAAVVDEPHAGALRQFGPLYVVNDVLPSLER
                     LGIAARDRSAARIVAVTGSVGKTSTKEALRLALADAGAVHASVASYNNHWGVPLTLAR
                     MPKDTAFGIFEIGMNHKGEITPLVAMVRPHIAIITTVAPVHIEYFASVDEIADAKAEI
                     FSGLIPGGVAIVNRDIAQYERLLAHAKDSPAGYVASFGEHEEADARLLSVALGSDHST
                     VTADICGEGLTYRLGAPGRHLAMNSLAVLLAAKAFGVDVKMAAAALSSLVSQPGRGER
                     LRLYAKNGPYTLIDESYNANPVSMAAAISLAGALPLEGKGRRIAVLGDMLELGQNAAK
                     LHKEVAADLVANNIDLVFAAGPLSKHLYNALPRHMQGGSRERSSELAPSVVAAVRGGD
                     IVLVKGSNAIKMNAIVDALRKHAAASLQDI"
     gene            complement(5296..6753)
                     /locus_tag="VNY06_07945"
     CDS             complement(5296..6753)
                     /locus_tag="VNY06_07945"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020174750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HWX14762.1"
                     /translation="MRLAELFPQADIPAALAGREIKGISADSRAVAQDMVFFAVPGTR
                     QDGLGYVQQAVRRGAVAIIAESDPGGPIGGAAFIKTENVRSALAHAAARMYPRQPETI
                     VAVTGTSGKTSVADFTRQIWMALGAEAASLGTLGVVSATGLASGTLTTPDPVTLHETL
                     DGLARRGVINLAFEASSHGIVQRRLDGVHVTAAAFTNLSRDHLDYHPTLEAYFGAKME
                     LFTRLLRPGDPAVVDADSDVAEKVIAACEQRGLRILSTGFHGEGIRLLEAKAESFATR
                     LKLTYGGMTYAPLMPLAGSFQASNALLAAGLCLVTGSPSHAVFAALENLKGAPGRLEL
                     VGRHKDAPIFVDYAHKPDALDKALQALRPFVRGKLIVVFGCGGDRDSGKRPLMGEVAA
                     RFADRVIVTDDNPRSEDPAAIRRAILQGAAGFDNVEEVGDRAAAIARAISMLDPEDGL
                     LIAGKGHETGQIIGAKTLPFSDSGCVGAVLEGLSG"
     gene            complement(6753..8552)
                     /locus_tag="VNY06_07950"
     CDS             complement(6753..8552)
                     /locus_tag="VNY06_07950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004448595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HWX14763.1"
                     /translation="MRGIESNNKEGPVFKQAGHRARVFRVSPFSSVLTVKLAKSTTRI
                     RLAAFVFLALYGTIAAKLLDFGLRPDRPASVKRAAADTIAAARPDILDRNGKVLATDI
                     KVMSVFAEPRRIVDKDEAVELLTAVLPNVDARDLRERLGSRKGFIWVKRAVTPQQQKE
                     LHHLGLPGIGFQPENKRVYPNGPIAAHVLGFANLDGVGISGLEKYIDGQGLADLHGAG
                     FSLTRDNLTPVTTSLDLEATYALRDELAKGIAKFRAKAGAAAILDVNTGEVIAMASLP
                     DYDPNSPGDALDPKRINQLSVGVYEMGSTFKAISIAMALDHGKVTLRSRVDARESLRY
                     GRFTIHDFHATHRVLSLPEVFTHSSNIATARMALMVGVDGHKAFLAKMGQLRRLRTEL
                     PESAEPLVPKNWGELNTITIAFGQGLNVAPLQAMMAVGALANGGTLVTPTFLKRSEEE
                     SKRDAPRVVKPETSEAMRYLMRLNAEIGTARIANVKGYFVGGKTGTADKIIHGHYANA
                     RVLTTFMAVAPADKPKYLFLTLLDEPQGLPETGGQRTAAYNAGLVAGKVIERTGQLLG
                     LPPRIDLPPQPFPLLARLGYAEANIPAHNKGGH"
     gene            complement(8586..8978)
                     /locus_tag="VNY06_07955"
     CDS             complement(8586..8978)
                     /locus_tag="VNY06_07955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020174748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX14764.1"
                     /translation="MVRFLNILAIASLIGSAIYAYSIKYETTLHAEKIVKLKHEIQRE
                     RDQIDMLRSEWAHLARPERIEALANRFLDLRPSALSQVVRADALPDKAPRTDEIGNKL
                     EALGLGLPTNTPGDTNALDSAPSATPAR"
     gene            complement(8980..>9495)
                     /gene="mraW"
                     /locus_tag="VNY06_07960"
     CDS             complement(8980..>9495)
                     /gene="mraW"
                     /locus_tag="VNY06_07960"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019403414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytosine(1402)-N(4))-methyltransferase"
                     /protein_id="HWX14765.1"
                     /translation="AHAIVVDRVKAPFASTAALAALIARVVPHKPGDLHPATRAFQAV
                     RIAINDELRELVRALAAAEAILKECGRLVVVTFHSLEDRIVKQFLNSRSGRGQTFSRL
                     LPYESAPPAPTFHLTGKQPITPSQREIAGNPRARSAKLRYGVRTAAPPRGIDDRLMAL
                     AELPQTQAGGR"
BASE COUNT         2169 a         2815 c         2731 g         1780 t
ORIGIN      
        1 aagaccgatc cgccgctcgg cgaggcagag gcaacgcggt gaacgtcctg cgccggaaaa
       61 tcgccgccat atttcgccgc ccgttcgtcg gtgacgaggt gaatctcaaa gccacgggcg
      121 atgagcgcgt gactgactgc agctgccgga aacaaatggc cgcccgtgcc gcccgcggcg
      181 accacgatcc gacagcggcc gctcatgcca acacttctgc ccctgcttgc gcaaggatcg
      241 cagaacctgg ccgcttgcgc agcaccgcga ttagaaagcc gattgcgagc gcgagagaaa
      301 tgagcgatga gccgccataa gaaatgaagg gcagcgtcat gcccttggcc ggtagaagat
      361 gaacattgac ggccatgttg atcgcgcttt gaataccgaa aagcaggacc aggcctgctg
      421 cagcaaagcg gcagaaaggg tcctcattgc gccttgccgc taataatccg cgcaaaatga
      481 taaaggcgaa aatcgcaacg atcaggagac aggcgagaac accaaattcc tcccccgtaa
      541 tggcgaaaat aaaatccgta tgggcgtctg gaagaatgcg cttgaccgtt ccttcgcctg
      601 gccctttgcc aaaccagccg ccggacagaa aacaatcgag cgccgtatca acctgaaatg
      661 tgtcggcgat cccgccgccg gcagcaccgg gatcaacaaa cttgagcacg cgggcgcgca
      721 catgcggaac caatttgtaa gcaagcaagg caccgcccaa gcccaggcca ccgatgcctg
      781 ccacccaaaa ccaatgcagt ccggccatga agaaaagcgc cgcccagacc agagagacaa
      841 gcatcgtctg gccaaaatcc ggttggagta ccagcggagt gatggtcatg agcagcagaa
      901 gaacagcaag aagcaccgcg gggacatcgc gccgcttcgc cccttccgaa aaggcccagg
      961 caacgagaat aacaaaggcc ggttttagga actctgaggg ctgaatgcca aaaatccagc
     1021 gccgcgatcc cttgacttct tggccaaaaa ataacgccac aatgatcaag gcaatcgata
     1081 ccagaaacac aaaaagcgcg acccggcgta cctgccgcgg ggaaagaaat gacgtcgcca
     1141 aaagcactgt gacggagggc acaaggtaaa gaacatgacg atgcacgaaa tgaaacgtcg
     1201 aaaggttgag ccgctccgcg actggcgggc ttccggccat ggttagcacg agcccgagca
     1261 tcatcagcgc tccaactgcg ccaagaatcc atcggtcgat cgtccaccac caatttgcca
     1321 ggtgagatcg ttccgcgcgc gataccattg tgccctctca gccctcagtt tccccagtca
     1381 gcaatgcctt gacgatggtc cggaaccagt ctccacgttt ctcgaaatct gcaaattgat
     1441 cgaaggaggc cgaggccgga gatagcagca cgaccggctc ttcgagatcg cttagctgcg
     1501 cgtcgcgagc ggccgcgaga accgccgtat ccagcgtgcc gcagcgctca aaaggaaacg
     1561 cgtccccgat cgttttggcg aaaagctcgg ttgcctcacc gatcagatag gccttgacaa
     1621 ctttaccgaa caacgcccgc agcggttcga tgccgcctgc tttcgccctg ccgccgacga
     1681 tccaaaaaac cttttcgaag gaaagaagcg ccttttcggc agcattggca ttggttgctt
     1741 tggaatcgtt gatgaaaaga acctttcctt tccgccccac ttcctccaag cggtgtgcca
     1801 gcccgggaaa acttcgcagg cctttttcga cttggccgca atcaagccct agaggcccaa
     1861 gtgccgcaac ggcggcggcg gcgttctggc cgttatgtct tccccgcagc gcggcaattc
     1921 cggcaagatc agcgaccatg gccaatttgc ctgcgctctg gcgcatgaga catgtgccat
     1981 cgagaaaaag ataatcattg caatatttga tcgcggtttt ggtcatggag atgcatgttg
     2041 tctttcgacc ggttttcatg cgttccgtgg caatcgcggc ggtgatcgca tcgtccattc
     2101 cggtaaccgc aagatcggcc ttttccaaaa gccgtgcttt gattgcggcg taactctcca
     2161 tggtgccatg ccgatcgagg tgatccgggg tcacatttaa tagcacgcca acggcaggat
     2221 cgagcgaagg ggcaagatcg atctggaacg aagagatttc gacgacatga acgcgatctt
     2281 ttgacggtgg cgctaaagcc aatattggcg tgccaatatt gccgccgact tcgaccttaa
     2341 attcaaagct ttgaaaaaga tgcgcggcaa gcgccgtcgt cgtagatttt ccgttcgttc
     2401 cggtaattgc tattagcggc gaagccggcg cgatttttcg ccgctcgcgg cagaacaatt
     2461 cgatatcgcc gataatttca acgccggcct tgcttgccat ttgcactgtc caatgcggtg
     2521 ccggatgggt aagcggaact ccgggcgaaa gcacaagcga cgcgaaggat gtccagtcgg
     2581 caagcgccag gttctcgata cgaagaccgg ccgcgcgcgc cttctcacgc gcgtgctcat
     2641 tgtcatccca aacggtgaca ttggcgcctc ccgcgacaag cgcttgcgcg gcaacgaagc
     2701 ctgaaccgcc aagaccaaac acggcggcat gtttgccatc aaagcaagtg acaggcgtca
     2761 tcgtcatctc agcttcaagg tggaaaggcc gatcagagcc agcacaaaag cgataatcca
     2821 aaagcgcacg acgacttgcg gctccgacca gccaagctgt tcgaagtggt gatggatagg
     2881 cgccatcttg aagacccgct tgccagtgag cttgaaggaa gcgacctgaa caatgaccga
     2941 caatgtttca agaacgaaaa gcccgccgac aatggcaagg acgatctcat gcttgacggc
     3001 gaccgctatg gtgccgagcg cgccgccaag agcaagggag cccgtgtcgc ccataaaaat
     3061 ctgcgcaggc ggcgcgttaa accaaagaaa acccaagccg gcgccaatca aggcgccgca
     3121 gataatggca agctctcctg ccccgggcac aaaattaatc ccgaggtagc ttgagaatat
     3181 cgcgtttccg gcaagatagg cgatgatccc aaaggttgcc gccgcgatca taaccggcac
     3241 aatcgcgagg ccatcaagac catccgtgag attgaccgca ttgcccgccg caacgatcac
     3301 aaaagcgcca aaggcaagaa agaaaacacc taggtcgacg gcaaaaccat tgattgcggg
     3361 cagagcgagg cccgtcacat gcggcgtgcc gagcttcatc atcgcaaggc atgcaatccc
     3421 tgcaataccc acttctattg caagccgccg ccgcccggaa aatcccttgt gcgtctgttt
     3481 tgtcaccttc aaaaggtcat catagaatcc gatcacgccg aaaccggctg tcaccaaaag
     3541 cacgatccag acaaaatgat tacgcaaatt ggcccagagc agggtcgata cgatcacccc
     3601 agatagaatc atcaacccgc ccattgttgg cgttccggcc ttggtcagga gatgcgactt
     3661 cggcccatcg tcgcgaattg gttggccctt gccttgtttt aggcgcaagg cggcgatgat
     3721 gcccggcccg aaaaagaaga caaagaagag tgccgtcgcg gtggcgccgc cggtgcggaa
     3781 cgttatgtag cggaacagat taagcggctg gaaaacgctt gcatattccg aaagccaagt
     3841 gagcattaga cgcttcctaa atgtcttgca gggatgccgc ggcatgtttc ctcagagcat
     3901 cgacgatcgc gttcatcttt atggcgttcg agcccttgac cagcacgatg tcaccgcccc
     3961 gcaccgcggc cacaacggat ggcgcgagct cgcttgatcg ctcccgcgag cctccctgca
     4021 tgtgacgcgg cagggcgttg tagagatgct tagacaacgg gccggcagca aagacgaggt
     4081 cgatgttatt tgcgaccaaa tcggcagcga cctctttgtg cagctttgcg gcgttctgac
     4141 cgagttctag catgtcgccg agaaccgcga tccggcgccc cttgccctcc aatggcagcg
     4201 cgcccgcaag cgagattgcc gccgccatcg acaccggatt ggcattgtag ctctcgtcga
     4261 tcagagtata ggggccgttt ttggcgtaga ggcgcagcct ctcgcctcgc cccggttggg
     4321 acacaaggga ggaaagagct gccgcggcca tcttgacgtc gacaccaaag gcctttgcag
     4381 caagaagcac ggcgagcgaa ttcatcgcaa gatggcggcc cggcgcgccc agacgatagg
     4441 tcagcccctc gccgcagata tctgccgtta cggtggaatg gtctgaccca agggcgacgc
     4501 tcagaagtct cgcatcggct tcttcatgtt cgccaaaact cgcgacatag cccgcgggag
     4561 aatctttggc gtgagcaagg aggcgctcat attgggcgat atcccggtta acgattgcaa
     4621 cgccgcccgg aatgaggcca gaaaaaatct ctgccttggc gtcggcgatt tcatccacac
     4681 ttgcaaaata ttcgatatga accggggcga ctgtcgtgat gatcgcgata tgcggacgca
     4741 ccatcgcgac aagcggcgtg atttcgcctt tgtggttcat gccgatttca aagatcccga
     4801 acgctgtatc tttcggcatt cgcgcgagag tgagcggcac gccccaatga ttattgtagg
     4861 aggcaaccga ggcgtgcacc gcaccggcgt cagccagcgc gagccgcaac gcctctttgg
     4921 tcgaggtctt gccgacggaa cccgttaccg cgacaattcg ggcagcggag cgatcccgcg
     4981 cggcaatgcc gagacgttcc aacgaaggga gaacatcgtt gacgacatac agtggcccaa
     5041 actgccgaag cgctccagca tgaggttcat cgaccacagc ggcaacagcg cccttgtcaa
     5101 atgcggcgct cacataatca tggccgtccg agttcggtcc tttgatagca aagaagagct
     5161 cgccttcctg aagcgagcgc gtatcgatcg aaattccggt gacgccgcca cgggggattg
     5221 aaccgctgac ccgcgccgaa agcgagccaa caagaccgag gctcgtccaa agaacgtcgc
     5281 tggcggtgcc ttcgctcatc cggaaagccc ctcgagcacg gcaccaacgc agccggagtc
     5341 cgaaaagggg agtgttttgg ccccgatgat ctgacccgtt tcatggccct tccccgcaat
     5401 caaaagacca tcctctgggt caagcattga aatcgcacgt gcgatcgccg cggcccggtc
     5461 gccgacctcc tcgacattgt cgaaacctgc cgcgccttga aggatggctc tgcggatcgc
     5521 agctgggtct tccgagcggg gattatcgtc tgttacgatg acgcggtccg caaagcgcgc
     5581 cgccacctcg cccatcagcg gtctcttacc agaatcgcga tcgccgccgc agccaaagac
     5641 aacgatgagc tttccgcgca caaacgggcg tagcgcctga agcgccttgt caagcgcatc
     5701 gggcttatga gcgtaatcga caaagatcgg cgcatcttta tggcggccga cgagttccag
     5761 acggccaggc gcgcctttca gattttcaag cgccgcgaac acggcgtgag aagggcttcc
     5821 cgtcacaagg caaagcccgg cggcgaggag cgcgttcgaa gcttgaaacg aaccagcaag
     5881 cggcataagc ggcgcatagg tcatgccacc ataggtgagt ttgagccggg tagcaaaact
     5941 ctccgctttg gcttcgagga ggcgtatccc ctccccatgg aagccggtcg ataggatgcg
     6001 aaggccgcgc tgctcgcaag ccgcaattac cttttctgcc acgtcgctat cggcatcaac
     6061 gacggcagga tcacccggac gaagcagccg ggtaaaaagc tccattttgg cgccaaaata
     6121 tgcctcaagc gtcggatgat aatcgagatg atcgcgtgag agattggtga aggccgcggc
     6181 agtgacatga acgccgtcaa gccggcgctg cacgattcca tgcgaagaag cctcaaaggc
     6241 gagattgatc actcctctgc gtgctagtcc gtcaagggtt tcgtgcaaag tcaccggatc
     6301 aggtgtggtg agtgtgcctg acgccagccc tgtcgcggaa actacgccca gagtgccaag
     6361 ggaggcagcc tcagcgccaa gcgccatcca aatctgacgc gtaaaatcgg caaccgaagt
     6421 ttttccactc gttccagtga ccgcgacgat cgtttctggc tggcgcgggt acatgcgcgc
     6481 agccgcatgc gccagggcgc tgcgcacatt ctccgttttg atgaaggcgg cgcctccaat
     6541 gggaccgccg ggatcgctct cggctatgat cgcgacggcg ccacgccgaa ccgcttgctg
     6601 cacatagcca aggccgtcct gccgtgttcc gggcacggca aaaaaaacca tgtcttgcgc
     6661 caccgcccgg ctgtcggcgc tgatgccctt gatctcgcgt cccgccaaag ccgctggaat
     6721 atcagcctgc ggaaagagtt cggcaagccg catcaatgac cgcctttgtt gtgagccggg
     6781 atattggcct cggcatagcc aagccttgcc aggagcggaa agggttgcgg aggtagatca
     6841 atgcgcggcg gtagaccaag caattgtccg gtccgctcga taaccttgcc ggctaccaga
     6901 cctgcattat aagcggcggt gcgctggccg cctgtctccg gcagtccttg tggctcatcc
     6961 aaaagcgtca aaaaaagata tttgggctta tctgctgggg ccaccgccat gaaggttgtc
     7021 aacacccggg cattcgcata atggccatga atgatcttat ccgccgtgcc tgtcttaccg
     7081 ccgacgaaat agcccttgac attggctatt ctcgcggttc cgatttcggc gttaagccgc
     7141 atcaaatagc gcatggcctc ggacgtttcc ggcttgacga cacgtggcgc atcgcgcttc
     7201 gattcctctt cgctccgctt caaaaaggtc ggagtgacaa gagttccgcc atttgccagt
     7261 gcgccaacgg ccatcatggc ctgcaatgga gcaacattga ggccctggcc gaaggcaatg
     7321 gtgatcgtat tgagctcgcc ccaattcttc gggacgagag gctcggccga ttcgggcagc
     7381 tcagtacgca gacggcgaag ctgccccatt ttcgccaaaa acgctttgtg cccgtcaacg
     7441 ccgaccatca gagccatgcg cgcagtcgcg atattggacg aatgggtgaa gacctcgggc
     7501 aagctcagaa cgcggtgcgt cgcatggaaa tcatggatcg tgaagcgccc ataccgcagg
     7561 ctttcgcgcg cgtcgactcg tgaccgcagc gtgaccttgc cgtgatcgag ggccatggcg
     7621 atggaaatag ccttaaaggt cgagcccatt tcatagacgc cgacggaaag ctggttgatg
     7681 cgcttgggat cgagcgcgtc gcccggcgaa ttggggtcat agtcgggcaa ggacgccatg
     7741 gcgatcactt ccccggtatt gacgtcaaga atggccgccg cccccgcctt ggctctgaac
     7801 ttcgcaatgc ctttcgcgag ctcgtcgcgc aacgcatagg tcgcttcgag atcgagcgat
     7861 gtcgtgaccg gtgtcagatt gtcacgggtc aggctaaatc cggcgccgtg caaatcggcg
     7921 agaccctggc cgtcgatata cttctcgagc ccggaaattc cgacgccgtc gagattggcg
     7981 aaaccaagaa catgcgcggc aatcggccca ttgggataga ctcttttgtt ttcgggctga
     8041 aagccaatgc cggggagccc aaggtggtga agctcttttt gctgttgcgg cgtaaccgca
     8101 cgtttgaccc agatgaagcc cttgcgggag ccaaggcgct ctcgcaagtc tcgggcatca
     8161 acattgggaa gcacagcggt caaaagttcg acggcttcat ccttgtcgac gatccgcctt
     8221 ggctcggcaa aaaccgacat gaccttgatg tcggttgcga gaactttgcc gttgcgatcg
     8281 agaatatccg ggcgcgccgc cgcaatcgta tcggcggccg cacgcttgac gctcgctggc
     8341 ctgtcgggac gcaatccgaa atcaagaagc ttcgccgcaa tggtcccata gagggcaaga
     8401 aagacgaatg ccgcgagccg aatgcgagtt gtgctctttg ctagctttac cgtgagcacg
     8461 ctggagaacg ggctcacgcg gaaaacccga gcgcggtgcc cagcctgttt gaaaactgga
     8521 ccctccttat tgtttgattc gatgccacgc ataataatgt cgcgacctcc ccctttgtcg
     8581 tgcgctcacc ttgctggcgt cgcggatgga gcggaatcta aggcgttggt atcgcccggg
     8641 gtattcgtcg gcagcccaag accaagcgct tccagcttat ttccaatctc gtccgttctt
     8701 ggcgccttgt ctggcagggc atctgcccgg acgacctgac taagtgcgga aggtcgtaaa
     8761 tcgaggaatc tgttcgcgag ggcctcgatc cgctccggcc gtgccagatg cgcccattca
     8821 gaccgcaaca tgtcgatttg atcccgttcc cgctgaatct catgtttgag cttcacgatt
     8881 ttttctgcat gaagggtcgt ctcatatttg atggagtaag cgtagatcgc cgaaccgatg
     8941 agagaggcaa tcgcaaggat attgaggaaa cgcaccattt cagcggcctc ccgcttgcgt
     9001 ttggggcagc tccgccagcg ccatcagccg atcatcgatc cctcgcgggg gagcagcggt
     9061 gcgcacacca tagcgaagct tcgccgaccg cgcgcgcgga ttgccggcta tttcgcgctg
     9121 cgagggcgta atcggctgct tgccagtaag atggaaggtt ggcgctggag gcgcggactc
     9181 atagggcaga agccgggaaa atgtttggcc ccgaccagaa cgtgagttca gaaattgctt
     9241 aacgattcgg tcctcaagcg agtgaaacgt caccacgacg agccgcccgc attctttcaa
     9301 aatcgcctca gccgctgcga gcgcgcggac gagttcgcgg agttcatcat tgatggcaat
     9361 gcgcaccgcc tgaaaagcgc gtgttgccgg gtgcaggtcg cccggcttat ggggcacgac
     9421 gcgggcgatc agcgcggcga gtgcggctgt cgaagcaaaa ggcgccttca cgcgatcgac
     9481 aacaatggca tgtgc
//
LOCUS       DATMPU010000347         4981 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_4280727,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000347 DATMPU010000000
VERSION     DATMPU010000347.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 4981)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4981)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4981
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4280727"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(94..390)
                     /locus_tag="VNY06_07965"
     CDS             complement(94..390)
                     /locus_tag="VNY06_07965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX14766.1"
                     /translation="MADSWLRFAIARRQAPAGFLPLAGLAFDLVGRLLAAAMAAMVSA
                     CAVGPDFVTPTAPVAPTWLEWRYKSLKTGDYEYRYWWRVFRDPALDRLIEIAYQ"
     gene            803..1975
                     /locus_tag="VNY06_07970"
     CDS             803..1975
                     /locus_tag="VNY06_07970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012589673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HWX14767.1"
                     /translation="MTTARPAVEKSHRRKERPPFERIALLLQGGGALGSYQGGAYQAL
                     AEAELHPDWIAGISIGTINAALIAGNPPEKRVERLREFWEIVSTPPLGLPSVASMDIR
                     NEYTHRLVNQFESLGTLLTGATGFFKPRVPPPYLYPPGRIEALSFYDVSPLKPTLERL
                     VDFDLINNGSTRLSVGAVNIRTGNLVCFDNANCDIGPEHVMASGSLPPGFPATEIEGE
                     YYWDGGLVSNTPLDWVLDSTPRQDTLAFQIDLWSARGDLPSNLMESETRQKEIRYSSR
                     TRAATDQFKKEQKLRRAAAKILAQIPRDLRQTPEAELLASGADEKVYNIVHLIYRSQK
                     YEGASKDYEFSRRTMEEHWKSGYHDTVHTLRHPEVLQRPTGEDGVFTFDLARDGRV"
     gene            2062..2820
                     /locus_tag="VNY06_07975"
     CDS             2062..2820
                     /locus_tag="VNY06_07975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006018861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetate decarboxylase"
                     /protein_id="HWX14768.1"
                     /translation="MKLSDVRAKAFAMPLTDPAYPPGPYKFYNREFVVISYRSDPEVM
                     REVVPEPLEVVGDIVNYEFIRMPDSTGFGDYTETGQVIPVRFRTKDGTGQEGGYVHAM
                     YLDDNSPIAGGREIWGFPKKLAKPKIAHESETIVCTLHYGSVLCVSATMGYKHRELDP
                     APLLKSLAKPAFMIKIIPHVDCTPRICELVRYYLEDVTLKGAWSGPAAIELFEHSMCD
                     VARLPVRGVVSATHFVTDLTLGLGEVVHDYLAEA"
     gene            2929..3656
                     /locus_tag="VNY06_07980"
                     /pseudo
     CDS             2929..3656
                     /locus_tag="VNY06_07980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012336161.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0043295 - glutathione binding [Evidence
                     IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
     gene            3789..>4981
                     /locus_tag="VNY06_07985"
     CDS             3789..>4981
                     /locus_tag="VNY06_07985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019404730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HWX14769.1"
                     /translation="MAKHDAIIIGTGQAGPSLAGRLAKAGMDVAIIERKFFGGTCVNT
                     GCIPTKTLVASAYAAHMARRAADYGVLTNGAVGVDMKAVKARKDAVSGKSKLGLESYL
                     KSLKGCTVYENHAQFISAKEVAVGDQVLTGDKIFVNVGGRALVPKIPGLDEIHYLTNS
                     SMMEVDFLPRHLIIIGGSYVGLEFAQIYRRFGSEVTVIELAPRLIAREDEDVSKAVAA
                     ILAREGVGVRTDAECVSVSADGKDVSVQLGCAKGPDTVTGSHLLLAVGRRSNTDDLAL
                     DKAGIAFNAKGYIEVDDFLQTSVPSIWALGDCNGRGAFTHTSYNDFEIVAANLLDNEQ
                     RRVTDRIKAYNLYIDPPLGRAGMTEAEVRASGRPALMATMPMERVGRAFEKGETQGFM
                     KILVD"
BASE COUNT         1115 a         1482 c         1369 g         1015 t
ORIGIN      
        1 cgcctgcctc tgcggccaga actcgccaat cgcaactccc agctgcgcgc gggcttggag
       61 caccctggta ccagcgctga gcagcgtcag attttattgg taggcaatct cgattaggcg
      121 atcgagcgcc gggtcacgga aaacgcgcca ccaataccgg tattcgtaat cccccgtttt
      181 cagcgattta tagcgccatt cgagccaagt aggcgcaacc ggagccgttg gtgtgacgaa
      241 atcggggcca accgcacagg cactaaccat ggcggccatt gcggcagcga gcaaccggcc
      301 gacgaggtca aaagcgagcc ccgcgagagg caaaaatcca gccggagcct gccgcctcgc
      361 gatcgcaaac cgaagccaac tgtccgccat gaactgccct ggtccatttg caaaattcag
      421 ccgtcgtgcg tttgattgtc atatcaagca ttggtcacac gggcgcgtgc gcaaaccccg
      481 tggatgcgcc acaaagctct ccttcgacta gcccggtaaa atgatggcag aacagtagca
      541 cgtggccgct cagcttgttg tgacaaatcg gcaaccactg ctgagaatca ccacaaatgt
      601 ctcgccggtt gaggaacagc cggagccgag taagctttga accaaaaagc cgtcgaagga
      661 cccgtttttg tgatattcct ccaaagaagg cttcgatatg ccactggttg cggccgcgtt
      721 atctcattgc tacagttcca caatatcgca tcattccagc cctgctgttt tgaaagcggg
      781 ttggcgaccg agaggactcc ttatgaccac tgcgcggccg gccgttgaaa aatcgcaccg
      841 ccggaaagaa cgccctccgt tcgagcgcat agcgcttctc ttgcaaggcg gtggggctct
      901 cggctcctat cagggcggcg cctatcaggc tctcgcggag gctgaacttc atccggattg
      961 gatcgcgggg atttcgattg gcacaattaa tgccgcgctc attgccggca atccgccgga
     1021 aaagcgcgtc gagagactgc gggaattttg ggagatagtc agcacaccac ctctaggcct
     1081 accctccgtc gcaagcatgg acatccgcaa cgaatatact caccggctcg tcaaccaatt
     1141 tgaatcgctg ggaacgctgc tcactggagc gacaggattc ttcaagccgc gcgtaccgcc
     1201 tccttacctt tatcctccgg gtcggatcga agcactaagc ttctatgacg tctctccctt
     1261 aaagccgacg ctcgaacgct tggtcgactt cgatttgatc aacaacggat cgacgcgtct
     1321 aagcgtcggc gcggtcaaca tccgtacagg caatctcgtc tgcttcgata atgccaattg
     1381 cgacatcggc ccggaacatg tgatggcaag cggctcgctg ccaccgggtt ttccagcgac
     1441 ggagatcgag ggtgagtact actgggacgg cggtctcgtc tccaacacgc ctttggattg
     1501 ggtgctcgac agcacgccgc gccaggatac gcttgctttt caaatcgatc tttggagcgc
     1561 gcggggcgat ctcccgagca atctcatgga atccgaaaca cgccagaagg aaatccgcta
     1621 ttcgagccgg acccgggcag ccacggatca gttcaagaag gagcaaaagc tgcgccgcgc
     1681 ggcggcaaaa atccttgcgc agatccccag agatctgcgg caaacaccgg aagccgaatt
     1741 gcttgcctct ggtgcagacg aaaaggtcta taacatcgtg cacctgatct atcgctccca
     1801 gaaatatgaa ggcgcatcca aggattatga attctcccgg cgaacaatgg aggaacattg
     1861 gaagtccggt taccacgaca ccgtccacac cttgcgacac ccggaagtcc tgcagcggcc
     1921 aaccggcgag gatggcgtct ttactttcga tcttgccaga gacgggcgcg tgtagcagac
     1981 ccgatcatca cgtacggacg ccgcttgccg aaatggcacg gctgactctc caatcatttc
     2041 ctaccaacgg agtccttgcc catgaagctc tctgacgtcc gcgcaaaagc cttcgccatg
     2101 cctctcaccg atcccgccta tccgccagga ccctacaaat tctacaaccg cgaattcgtg
     2161 gtgatcagct accgctcgga ccccgaagtc atgcgcgaag tcgtccccga gccgcttgag
     2221 gtcgttggag acattgtcaa ctatgaattt atccgcatgc ccgattcgac aggctttggc
     2281 gactacaccg aaaccgggca agtgatcccc gtgcggtttc gcaccaagga cggtacggga
     2341 caggagggcg gttatgtgca tgccatgtat ctcgacgaca attcgccaat cgctggcgga
     2401 cgggaaattt ggggatttcc aaaaaagctc gcgaaaccaa aaatcgcgca tgagagcgag
     2461 acgatcgtgt gcacacttca ctatggctcg gtgctctgcg tgtccgctac catgggctat
     2521 aagcaccgcg agcttgaccc cgcgcctttg ctcaaaagcc tcgcaaaacc cgctttcatg
     2581 attaagatca tacctcatgt ggattgtacg ccccgcattt gcgagcttgt ccgttactat
     2641 ctcgaggatg tcactttgaa aggcgcttgg tccggacctg ccgcaataga attattcgag
     2701 cattccatgt gcgacgttgc ccgcttgccg gttcgcgggg tcgtctcggc tacccacttc
     2761 gtcaccgacc tgacactcgg tctcggcgag gttgtacatg actaccttgc cgaggcttag
     2821 tagccctcgc gacgtgaaga ggcgttgcat tcctcactct tgcggtgctc ccagcgtagt
     2881 tttgacctag cgagcgtggg aaaacagcga gcacgcacgg agtttccgat ggcctatgag
     2941 ctctactact ggcccggtat tcagggacgc ggcgaattcg tccggctggc gctggaagaa
     3001 gcaggcgccg attacatcga tatggctcgc aaaccagaaa gcgagggcgg cggcgctgat
     3061 gccatcatgg ccttccttga gcgggacgac atcgcctgcc cgccatttgc cgcgccattc
     3121 ctaaaggacg gaaatctcgt gatcggccag accgctaata tattgctcta tctcggcccc
     3181 cgccatggcc tctctccaag tgacgaagaa aagcgccttt gggtccacca gatccagctg
     3241 accatcgccg acctgggggc gaggcccacg attcccacca tccggtaagc gatgagctct
     3301 attacgaaga ccaggagccc gaagccaagc gccgcgccga gcatttccgg aaggaccgaa
     3361 tccccaatta tctcgcctgg ttcgagcgca ttctggccca caatcccgat ggctccgcgc
     3421 agctcgccgg cagcgccgta acttacgcgg atttatcgct ctttcaggtt gtcgatggcc
     3481 tgctctatgc ttttccgcaa aggatggagc aggtgctcac cgagatccca cgcgtcgcgg
     3541 ctctgcatca cgaaattgcg caacgcccgc gtattcgtgc ctatcttgcg agcaatcgtc
     3601 gcatcccatt caacgagcaa gggatattcc gccgctaccc agagcttgat gggtagtctt
     3661 ttcttttccc gcgtgccaaa actgccctat agcgtccgcc tatcgaagcc aaagcaagca
     3721 ggcatttgca ccgccgagcg aatcgaacca tctctttacg gcaaatgaaa tgccacaaag
     3781 aggcaaccat ggccaagcat gatgccatca tcatcgggac cgggcaggca ggaccttccc
     3841 tagccgggcg ccttgcaaaa gccgggatgg atgtcgccat catcgagcga aagttttttg
     3901 gtggaacctg cgtcaatact ggctgcattc caaccaagac tttggtggcc agcgcctacg
     3961 ctgcccatat ggcacggcgc gcggcggatt acggggtgct caccaatggc gcagttggcg
     4021 tcgatatgaa ggccgtcaag gcgcgtaagg atgcagtttc gggtaaatcc aagctcggac
     4081 tcgaaagcta tttgaaaagc ctcaaaggct gcaccgtcta tgagaaccat gcccagttca
     4141 tctccgccaa ggaggtggcg gtcggcgatc aggttctgac tggcgataag atattcgtca
     4201 atgttggtgg ccgagcgctt gtgccaaaaa taccggggct cgatgaaatc cattacctga
     4261 caaacagttc catgatggaa gtcgattttc tcccgcgcca tctgatcatc atcggcggca
     4321 gttatgtcgg cctggagttt gcgcaaatct atcgccgttt cggaagcgaa gtgacggtga
     4381 tagaattggc cccccggctg atcgcacgcg aggacgaaga cgtttcgaaa gcggtggcgg
     4441 ccatcttggc gcgcgaaggc gtcggcgtgc gcacggacgc cgaatgcgtt tcggtgtcag
     4501 cggacggaaa agatgtttcc gtgcagctag gttgcgctaa ggggccggat acggtcaccg
     4561 gctcgcacct cctccttgca gtaggcaggc ggtcaaatac ggacgacctc gcgctcgaca
     4621 aggcaggcat tgccttcaac gcaaagggct atatcgaggt cgatgatttt ctccaaacga
     4681 gcgtgccaag catttgggct cttggcgatt gtaacggccg cggcgccttc actcacacat
     4741 cctacaatga ttttgaaatt gtcgccgcga atttgctcga caacgagcag cgccgtgtca
     4801 cagaccgcat aaaggcttat aatctatata ttgatccgcc gcttggccgg gccggcatga
     4861 cggaggccga agtgcgcgcc tccggcagac ccgcgctcat ggcaacaatg ccgatggaaa
     4921 gagtcggccg cgctttcgaa aaaggcgaga ctcagggatt tatgaaaatc ctcgttgacc
     4981 a
//
LOCUS       DATMPU010000348         3471 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_4598094,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000348 DATMPU010000000
VERSION     DATMPU010000348.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 3471)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3471)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3471
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4598094"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(391..1092)
                     /locus_tag="VNY06_07990"
     CDS             complement(391..1092)
                     /locus_tag="VNY06_07990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020174136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX14770.1"
                     /translation="MHYGNLSLRYHWMGMLVTFNLCAATISAEPVLRRTGTPWPDTLL
                     ARVEALALIETLNARLLGAHTATEVLDKWCADHKMASDPVVHARRIGNMPKPVSPEQR
                     QRLQIGLDEEVVYRRVELTCGGHVLVEADNWYVPARLGADIKHVLATTDTPFGRAVQS
                     LNPIRENFLVEILWKPLPDGWEMGLEPTDQPGKELALPWQLFQHRALIYNNAHEPFSE
                     VNEVYTREVLAFGPP"
     gene            1232..2002
                     /locus_tag="VNY06_07995"
                     /pseudo
     CDS             1232..2002
                     /locus_tag="VNY06_07995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007603048.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
     gene            2203..2784
                     /locus_tag="VNY06_08000"
     CDS             2203..2784
                     /locus_tag="VNY06_08000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012591944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaA family protein"
                     /protein_id="HWX14771.1"
                     /translation="MFQRLYHWTLSLAESPHAPWALGIIAFAESSFFPVPPDTILIPM
                     SLAKPKRAWVYALICTVGSVGGAVLGYVIGALLYETVGKWLINLYGYGARVDQFRALY
                     AQWGWAVILFKGLTPIPFKIVTITSGLLAYSLPLFILLSFLTRGARFFLIAVLLKYFG
                     EPINGLLEKYFGWFLAILALAVLVGFWIAARVI"
     gene            2781..3281
                     /locus_tag="VNY06_08005"
     CDS             2781..3281
                     /locus_tag="VNY06_08005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020174139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein B"
                     /protein_id="HWX14772.1"
                     /translation="MTVAMRLNPPLLIALSILAAAALSIAGAFVFEALGFAPCELCLT
                     ERIPYYAAIPLAGVAALFAARGHKKLLGAAFAGLALTFAASAVLGVYHAGIEWGFWPG
                     PKECSGVLERARSVEDFLVQLQSAKVVRCDAPALRIFGISLASWNAVVSVGLAVLARA
                     GLRAKL"
     gene            3313..3447
                     /locus_tag="VNY06_08010"
     CDS             3313..3447
                     /locus_tag="VNY06_08010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX14773.1"
                     /translation="MKHLLSTALLSRNVGAQNESLTKSYHRALWTITDYAPTLAQWAL
                     "
BASE COUNT          683 a          951 c         1028 g          809 t
ORIGIN      
        1 gccatccaat ttccaagcgg tttagcgagc tccatccggt cataatattt taaccggtgc
       61 accaattgcg gcaccggtcc ttcgtcgaaa ttcgcgactg cccgggcgcg gacaaagacc
      121 ggaggattgc tcaccgcctc aggcgaaagc agaccgtcac cggccgatcg caaaaagggc
      181 gctcgataaa acgcactcgc gcccagcagg cgggcagaga gcgcctgggg tctccgtgga
      241 tcgaactcag gcaggcaggg ggaaacacaa tatcggcggc agcgcgcatg aggtcgcgca
      301 gtcgcgccaa ggtacgaggc gggacggcat aatgccgcag actggataga gcctgcgaaa
      361 ccggctccat tgaagccatg ccacgcgccc tcaaggtggt ccgaatgcca aaacttcccg
      421 cgtataaacc tcatttacct ccgagaaagg ctcatgcgcg ttattataga tcaaggcgcg
      481 atgctggaag agctgccagg gcagggccag ttctttcccg ggttgatccg tcggttcgag
      541 ccccatttcc catccatccg ggagcggctt ccacagaatc tccaccaaaa aattctcgcg
      601 gatggggttt aggctctgca ccgcgcggcc gaagggtgtg tcggtcgttg ccaaaacatg
      661 tttgatatcc gcgccgagcc ttgccggcac ataccagttg tcggcttcca caaggacatg
      721 gccgccgcag gtgagttcga cccgccggta aacgacttct tcatccagac cgatttgaag
      781 tctctggcgt tgctcggggg aaaccggctt tggcatattg ccgatgcgcc tcgcatggac
      841 gacgggatcg ctcgccattt tgtggtcggc gcaccatttg tcgagaactt ccgtcgcggt
      901 atgggcgcca aggaggcggg cgtttagggt ttcgatgaga gcaagagcct cgacccgcgc
      961 aagcagagta tcgggccagg gcgtgccggt ccgtcttaag acgggttcag cggagatcgt
     1021 agcggcgcaa aggttgaacg tcaccagcat tcccatccag tgatagcgca ggctcaggtt
     1081 tccataatgc attgtgcggt accgtgacaa ccggacggat aaatggaata gcatagactt
     1141 atcgattgcg ccccattgcg cggggctgga aaatcatttc gccttgaaca aattataatt
     1201 atttggtttc ttagcatttt ggggagcgca cgtgcacatc gaccttggcg gtgaaatcgc
     1261 cttagtcacc gcttcgaccg ccggcattgg ccttgctaca gccaaagggt tagcggccgc
     1321 aggcgcaatg gtcatcgtca atggccgcac gcaagggaga gtcgaggcgc tggagcccgg
     1381 tgccaaggtg gtcggcattg tcgcagatgc gagcaacgca aatggttgcg atgctttggt
     1441 tacgcagcat ccttattgcg atatcctcgt taacaacctc ggaatctacg cagaacagga
     1501 cttttttgcg cttcccgaca gcgaatggct tcgctttttg gaagtgaacg ttatgtccgg
     1561 cgtgcgcctg tcgcgcgcct atctacctgg aatgattgcc agaggctggg gccgcattgt
     1621 ctttatctcc tcggagtcgg cgctaaatgt tcccgcggac ttgattcact atgctgtaac
     1681 caagacggcg cagctttctg tcgcgcgcgg tctcgccaag cgtctcgccg gagccggggt
     1741 gacggtcaat tccattttgc cagggcccac gctcacggag ggggtagctg aaatgcttaa
     1801 ggatagaaaa gttccgggcc aatcgctgga agatgccgct gcggcatttg tctaggagaa
     1861 tcggtcgacg tcaatcattc agaggatggc cagcgcagac gaagtcgcca atatggtcgt
     1921 ttatgcttgc tcgtcgcagg catccgccac cacaggggcc gctctgcgcg tggatggcgg
     1981 cgtggtcgat accatcgcct gaggaataat gatacaatca agcctcatcc tgcagggcga
     2041 gcctcgcggg tctgacgtcg gatgacgcgt ggctgttgcg cgccgcgttg atttaggcaa
     2101 gataaagcaa catcaagata tagcaagata taggaagata tgcgttttcg cttcttcctg
     2161 ctttctcctc aaggcgcctc tcccgctcgt gtacggccga acatgttcca aaggctttac
     2221 cattggactc tgagcctggc cgagagcccg catgcgccct gggcgctcgg tatcattgct
     2281 tttgcggaga gctcgttttt tccggtgccg ccggacacga ttttgatacc gatgtcgctt
     2341 gcgaagccca agcgcgcatg ggtctatgcg ctgatttgca cggtgggttc tgtcggcggc
     2401 gctgtgcttg gctatgtgat cggcgcgctg ctttatgaga cagtcggaaa gtggctgatc
     2461 aatctctatg gatatggcgc gcgtgttgat caattccgcg cgctttatgc acaatggggc
     2521 tgggccgtca ttctcttcaa aggtctgacg ccgatcccat tcaaaattgt gacgatcacg
     2581 agcgggcttt tggcatacag cctgccgctc ttcatactcc tttcatttct cacccgcggc
     2641 gcaaggtttt tcttgatagc tgtgttgctg aagtattttg gcgagccgat caatggtcta
     2701 ctcgaaaaat attttggctg gtttctggcg atccttgcgc ttgccgtgct tgtgggattt
     2761 tggatcgccg cgcgtgtaat ttgacagttg cgatgcgcct caaccctccc ttgctcattg
     2821 cgctttctat tctggcggca gcggccttat ccatcgcggg agcctttgtc tttgaggcgc
     2881 ttgggtttgc gccctgtgag ctctgcctta cggagcgcat cccctattat gcagccatcc
     2941 ccttagctgg tgtggcggcc ttatttgccg cgcgaggcca caagaagctt ttgggcgcgg
     3001 ccttcgcggg gctggcgctc acctttgccg cgagcgcggt ccttggcgtc tatcacgcgg
     3061 gcattgaatg ggggttttgg cctggaccaa aagaatgcag tggcgtgctc gagcgtgccc
     3121 gctcggttga agattttttg gtccaattgc aaagcgccaa ggtcgtgcga tgcgatgccc
     3181 cggcgctgcg gatatttggg atatcgctgg ccagctggaa cgcggtggtc tcagtagggt
     3241 tggctgtgct cgccagagct gggttacgtg ctaagctcta gcccaattct aacgccaagc
     3301 aggaaaacgc agatgaagca cttactctcg acggcgctgt tgtctcgcaa tgtgggagcg
     3361 caaaatgagt ccttgaccaa atcctatcac agagccttat ggacaatcac tgattatgca
     3421 ccgaccctcg cgcaatgggc cttgtgatca ttctttgtcg gtgatgcggc a
//
LOCUS       DATMPU010000349         3452 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_570850,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000349 DATMPU010000000
VERSION     DATMPU010000349.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 3452)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3452)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3452
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_570850"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..194)
                     /locus_tag="VNY06_08015"
     CDS             complement(<1..194)
                     /locus_tag="VNY06_08015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX14774.1"
                     /translation="MAGVGPAGTGVATLGVGVLAGAAAGDGTAGLGVAVAGEGASGTG
                     RLARWWLAWRRLARERLARER"
     gene            complement(459..1697)
                     /locus_tag="VNY06_08020"
     CDS             complement(459..1697)
                     /locus_tag="VNY06_08020"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012336271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HWX14775.1"
                     /translation="MASETKDVKRVVLAYSGGLDTSIILKWLQTTYGCEVVTFTADLG
                     QGEELEPARQKALLLGIRPEHIFIEDLREEFVGDYVFPMFRANAQYEGLYLLGTSIAR
                     PLIAKKQIEIARLTGADAVAHGATGKGNDQVRFELSYYALEPDIKVIAPWREWTLTSR
                     SALIAYAEQNQIPIAKDKRGEAPFSVDANLLHVSSEGKVLEDPSAEVPDYVYSRTLNP
                     EDAPDKAEYIEMDFERGDAVGVNGKPMSPANLLAHLNDLGRKHGIGRLDLVENRFVGM
                     KSRGIYETPGGTILYYAHRGIEQLTLDRGAAHLKDELMPKYSELIYNGFWFAPEREML
                     QALIDRSQEFVTGRVRLKLFKGLVSVTGRSSPYSLYDQDLVTFEEGASAYDHRDAVGF
                     IKLNALRLRTLAKRARKIGA"
     gene            2081..2797
                     /locus_tag="VNY06_08025"
                     /pseudo
     CDS             2081..2797
                     /locus_tag="VNY06_08025"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
     gene            complement(2887..>3452)
                     /locus_tag="VNY06_08030"
     CDS             complement(2887..>3452)
                     /locus_tag="VNY06_08030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004446456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit beta"
                     /protein_id="HWX14776.1"
                     /translation="SAAPAGVTETETRPVKQGSAEDAARILLAAKRVIVVPGYGMAVA
                     QAQHAVREMADILKSKGIDVKYAIHPVAGRMPGHMNVLLAEANVPYDEVFELDHINPE
                     FSDADVAVVIGANDITNPAAKTNSSSPIYGMPILDVEHAKDVLFVKRGLGAGYAGVEN
                     ELFFRNNTTMLFGDAKKMVDGIVKAMG"
BASE COUNT          717 a         1057 c          940 g          738 t
ORIGIN      
        1 cgctcccgtg ccaaccgctc ccgtgccagt cgccgccatg ccagccacca ccttgccaac
       61 cgccccgtgc cagacgcccc ctcgccggcc accgccacgc caagcccagc cgttccatcc
      121 ccagccgccg ccccagccaa aaccccgacg ccaagcgtag ccacaccagt accagccggg
      181 cccacgccag ccattgccat atcagcaata gttacggccg ccccaaacat attgcgtctt
      241 ctgcatgggg atcgcttcgc ttgctgccgt tgcgataccg gcattcggaa tcatggctgc
      301 gcgcgcgtcc ccgcccgttg ttgcggccgt cgcggatgca gccgtagccg cgactcctcc
      361 ctaagattac cggtctgggc cgaccaaaaa cggagattcc tcaatccgcg tgacgaagtc
      421 aattgaggtg attgaaatct attttcggcc cagacggctc acgcgccaat cttgcgagcg
      481 cgtttggcca gcgtgcgcaa gcgcaaggcg ttgagcttga tgaatcccac cgcgtcacga
      541 tgatcatagg cgctggcgcc ctcctcgaag gtcacgagat cctgatcata gagggaataa
      601 gggctcgaac gcccggtgac ggagacgagg cccttgaaaa gctttaaccg cacccggccc
      661 gtcacgaatt cctggctgcg atcaatcaaa gcttgcagca tttcgcgctc gggcgcgaac
      721 caaaagccat tatagatgag ttcggaatat ttcggcatga gctcatcctt aagatgcgcg
      781 gcgccgcggt cgagcgtgag ctgctctatg ccgcgatgcg catagtaaag gatcgtgccg
      841 ccaggcgtct cataaatgcc gcgcgatttc atgccaacaa agcggttctc gaccagatcg
      901 agccgcccga tgccatgctt gcgtccaaga tcattgagat gcgcgagcag atttgcaggc
      961 gacatcggct tgccattgac gccgaccgca tcgccgcgtt cgaaatccat ctcgatatat
     1021 tcagccttat ccggcgcatc ctccggattg agcgtgcgcg aatagacata atccggcacc
     1081 tccgcggagg gatcttccag cactttgcct tccgatgaga catggagaag attagcgtca
     1141 acggaaaatg gcgcttcgcc gcgcttatcc ttggcgatag gaatttggtt ttgctccgcg
     1201 taggcaatga gcgcggagcg tgacgtcaat gtccattccc tccacggggc gatgaccttg
     1261 atatcgggct cgagcgcata ataggaaagt tcaaaccgca cttgatcatt gcccttgccg
     1321 gttgcgccat gggccacggc gtcggcgccc gtcagtcttg caatctcgat ctgtttcttc
     1381 gcaatcaacg ggcgcgctat tgaagtgcca aggagataga ggccttcata ttgcgcattg
     1441 gcgcgaaaca tcgggaagac atagtccccc acaaattcct cacgcaggtc ctcgataaaa
     1501 atatgctcgg gccgaatgcc aagaagaagc gccttctgcc gcgcgggctc gagctcctcg
     1561 ccctgcccga gatcagctgt aaaggtgacc acttcgcagc cataggtggt ctgcagccat
     1621 ttgaggataa tcgacgtatc aagcccgcct gaataggcga gcacgaccct tttcacgtcc
     1681 tttgtttctg aagccatgga gaacccgcgg ggatgaaaga atcctgcgac tatagtggcg
     1741 gcgccgggct gcgcaagggc ggcaatgcgg ccgctcgatc gcacgtttga tgagcgctat
     1801 ttttcgggct tgcgccaaac tttccgtgct gtgcgccaaa ccatcatggt taagcggtgc
     1861 ttaagtaatt cacttcagtc ttcttcgcat atcggctcgg aaagctgcgg ccgggctcaa
     1921 aaaggttgac tctaatgaat tgcaaagatt tcacccgaaa acacgctctg gcgctggcaa
     1981 cgattagttt cgtcccgctc gccacgttgg gaaatttgtc cctgccagca gaaggcgcgg
     2041 attacagcag ccttttcccg atctcgcccc cacttacgac aaggtggact tcgcctccgg
     2101 ctggtatgtg cgcggcgata ttgcctatgc gcaggagagc tttcccgcga taacgcccgc
     2161 tcttgcccta accccgtccg tgttcaacac ttacagcacg ggggccggga tggggtacaa
     2221 ggtcaatgat tggttgcgga cagacctcgt tctcgactac cgttcaccgg tccaatcagc
     2281 tggaattggg gctgcgagga gctgccagat cgtgctaccc gacgtggtaa ccgggctgcc
     2341 taccccgacc gatcaaacgt gcttcggcca tttcaatacc cagattcatc gctgggacct
     2401 tctggccaac gcctatatag acatcggcac ctggcaaggc tttacgccct atgtgggagc
     2461 tggtgcgggg gtcagttggg ctcgaatctg gcaatccgtc aattggacct taagtaatgg
     2521 tctggcttgt caagcaaact gcggtttccc ggccagcggc ggcgcctttt tctatgcgga
     2581 ttgggaccgc aaccaatcga gcatgaccta tcgctttgcc tgggcgctta tggctggggt
     2641 tgccgtcgcc gtcgcggatc acgtgcaact tgacgttgga tatcggttcc tcgatctcgg
     2701 ttccgtcagc ggcatatcga gcgtaaccgg tacccctatc acccagaatg ttcgcgttaa
     2761 tgaggtgcgt ggcggcctgc gctacatgat cgactaggcc agcaaggctg gaacggtgtg
     2821 aggcgaatag ctggtaactt cgcctcacac caaattcagc ccccgcaatg gtcgcgccca
     2881 gcagcttcag cccatcgcct tcacgatgcc gtccaccatc tttttggcat cgccaaaaag
     2941 catcgtcgta ttgttcctaa aaaacaattc gttctcgacg ccggcgtaac cggcgcccag
     3001 cccgcgcttg acaaaaagca catccttggc gtgctctacg tcgagaatgg gcatgccata
     3061 gatcggcgag gacgaattgg tttttgcggc agggtttgtg atgtcattgg cgccgatgac
     3121 aacggcgaca tcggcatcag aaaactctgg attgatatga tcgagttcga aaacttcgtc
     3181 ataaggcaca ttggcttcgg cgaggagcac gttcatgtgc cctggcatgc gcccggcgac
     3241 cggatgaatc gcatatttga cgtcgatgcc cttgcttttc aatatgtccg ccatttcgcg
     3301 cacggcatgt tgcgcctgag cgaccgccat cccataaccc ggcacgacaa tcactctctt
     3361 cgcggcaagc aaaattcttg cggcatcctc cgccgagcct tgcttgactg gccgcgtctc
     3421 tgtttcggta acaccggcag gcgcggccga at
//
LOCUS       DATMPU010000350         3343 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_2425674,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000350 DATMPU010000000
VERSION     DATMPU010000350.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 3343)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3343)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3343
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2425674"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(444..628)
                     /locus_tag="VNY06_08035"
                     /pseudo
     CDS             complement(444..628)
                     /locus_tag="VNY06_08035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003506676.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(725..916)
                     /locus_tag="VNY06_08040"
     CDS             complement(725..916)
                     /locus_tag="VNY06_08040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX14777.1"
                     /translation="MVTVPAKLSFVELKPKIVKIPGPDHPITIEPNARQVVVKVANRI
                     VADTRDALTLQEASYTPVQ"
     gene            complement(1051..1308)
                     /locus_tag="VNY06_08045"
     CDS             complement(1051..1308)
                     /locus_tag="VNY06_08045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX14778.1"
                     /translation="MGWNDYIDVRQGASPPIYIDGIITELKREGWIEDKTFIPWASSD
                     GSQDGVCRFTKAGEGHDVEIIRYYPKNKSPYYEAVSVPKMH"
     gene            2235..2996
                     /locus_tag="VNY06_08050"
     CDS             2235..2996
                     /locus_tag="VNY06_08050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020177113.1"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HWX14779.1"
                     /translation="MFAAAGLALFTWMQLNAARAEKALSPKDSVPIITTNGEASAEVV
                     PDIATISLGVDTERPKAADAARENTRAAQAIVNEIKAQGVDAKDIKTISVTLAPVYDE
                     ERDANGFVTKRTLRGYIANDSLTVRVKDIGKAGTLASQMMDKGANRFNGITYEYSQKE
                     EKIDSLRGDAVRDALRKANSYVKALGIRLGRVLEIASQPHASVPVAARMMAAAAPARE
                     AAVSIPVEPGIETLRTEVQVTWNSPRISGYFRFAF"
     gene            complement(2975..3141)
                     /locus_tag="VNY06_08055"
                     /pseudo
     CDS             complement(2975..3141)
                     /locus_tag="VNY06_08055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012066732.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
BASE COUNT          737 a          930 c          912 g          764 t
ORIGIN      
        1 tcgcctacat cttcttgcgc ccagcaaggc agcgtcaacg cactggctac cagtgcgagc
       61 ccgccgacgg cggcaaataa gatgattttc ttcgtcatag aaccctcttg atgttgtaag
      121 tgcatcttta atttcagaat catctatttc tttaaatccg tatgtaaggt ctttcctcgg
      181 cattcggaat tgaaaagtct ttccagacac cgattctaaa taccaaccga atcaaacgtc
      241 gcacagcgga ggcttcccgc ctacgaagcg atatcgtgga tgaactgcga tcatcccctt
      301 aaatacatta caatttgaat ttcttcctca gatggctcaa aattcagccg atgggccgct
      361 tactaagcga tcgcccccgg ttatgcgttg gcagtcaggc aaccttgatt ggtatcgggc
      421 gagcggtcgc cctctccgcc gtgctaggcg aagaccatcg gcatcgatcg aagcttggcc
      481 agctttcgta agttctgagc ggtggcggcg agcaggaact catctctcgc cccgccgggt
      541 ccacgcagac gcagacgacc aacctgagga tgcgtttgag atgggcgaag agcatctcga
      601 ttttcttgcg cagatgtcgc gaccagacaa tcggacttgc tcgcgcgata gagccgcgtc
      661 ccatccttgg tgacgcctga tctcggcgtc ctgaagctcc gacggaactg caccaggagc
      721 ttgcctactg gacaggcgtg tagctcgcct cttggagcgt caacgcgtcc cgcgtgtcgg
      781 ccacgatgcg gttcgccacc ttcactacga cctggcgggc gttgggctcg atagtgatcg
      841 gatgatccgg gcctgggatc ttgacgatct ttggcttcag ttcgacgaaa ctaagtttag
      901 ctgggaccgt aaccatgaca gtaaaggggc agtttggagg ctcgccgcaa aatggcgacc
      961 ccctttgctg cccagtgccg tattcgcgga ctcgatcatt ccgcaaatgg ccgccgccgc
     1021 gacgatcagt ctatacgtcg ccaccaccgg ctaatgcatt ttaggcacac taaccgcctc
     1081 gtagtagggc gatttatttt tcggatagta ccgaataatc tctacgtcat gcccttcgcc
     1141 ggcttttgtg aagcggcata cgccatcttg tgacccgtcc gagctggccc aaggaataaa
     1201 agtcttgtct tcgatccaac cttctctttt caactccgtg attattccat cgatataaat
     1261 aggaggtgat gccccctgcc gtacatcgat ataatcgttc caacccatgg gtgttctctt
     1321 tcaactcaag acctaagcgg gtcttgccac aatcgcgcgc actgcaaaaa ggctatgagt
     1381 gcatcggcgc gctcggttgc agcgctgtcg gcgtattgcg caaccaatac gcagaaagcc
     1441 tagccgcgtt gctcatcgcc ctccgtgaat tccacttcga atgcatcgag gaacaacttg
     1501 acgaaccgag ttgcgcgctc gcggtatcga tatgactgag ctacctatgg cacgtcggct
     1561 gcagccaatt tcgttcctat tcattagcca gaatcgggga aatatatgtc ttcgaaagac
     1621 ctcaccgttc tttttggttt ggccgtggct tacgcgccac accgctcgga tcgaccttcg
     1681 acggtcagga gcgctccttg gcggtcggct cggaggcgcc gagcctgttg gcaggcccga
     1741 tcgtcgatgc cgacggcaac cctctgacgc caacccagat ctgaatccga ggtgatgatg
     1801 acccgaacaa cactatggct tgtaagcgcc gttttttata ggcggaagct ctctcgcttc
     1861 aagatcaggc cagatccgag gacatggcta caagccctca aagactacgc ccgctaccgt
     1921 ataagccgcc gtcatcaccc agaaccggtg cgcagcgacg taggacgtca atccggcgag
     1981 cgaattaggg acggcaggga cgttatctcc cggctgcaaa gttggttcgg cgaggctaga
     2041 catttttggg gcgcgttctt agcatgctgc ggcagatttc ttcataagcc tttccgtcag
     2101 aagtctgcca gaactcgaaa ttgtggtggc tcgggaagca ccattttcag tagactatca
     2161 gattcgagcc agttcggctt gcggagacat tcttcatgtt gatcaatctt tgcagtcatc
     2221 aatttggacg gtctatgttc gccgctgcag ggcttgcgct ttttacttgg atgcaattga
     2281 acgccgcacg cgctgagaaa gctctctcac ccaaagactc tgtacccatt atcacgacaa
     2341 atggagaagc gagcgcagag gtcgtgcccg atatcgcgac gatttcgctt ggggtcgata
     2401 cggagcggcc aaaggcggcc gatgcggcgc gtgaaaatac tcgcgcggcg caggcgatcg
     2461 tcaatgagat caaggcccaa ggggttgacg caaaggatat taagaccatc tcagtgaccc
     2521 tcgccccagt ctacgacgaa gagagggacg ccaatggctt tgttaccaaa cggactttgc
     2581 gcggttatat tgccaatgat tcgctgactg tgcgagtcaa ggatatcggc aaggctggca
     2641 cccttgcgag ccaaatgatg gataagggcg ccaatcgttt taatggcatc acttatgaat
     2701 attcgcaaaa ggaggagaaa atcgattcct tgcgcggcga tgccgttcgc gatgccttgc
     2761 gcaaggccaa tagctatgtc aaagcgcttg gcataaggct tgggcgggtt ttggaaatcg
     2821 cctcgcagcc tcatgcctcg gtgccggtag cagctcggat gatggccgcc gccgcgcccg
     2881 cgcgggaggc cgctgtttct attcccgttg agccgggaat cgagactttg cggacagagg
     2941 tccaagtcac ctggaactcg ccgaggatta gcggttactt tcgatttgcc ttttgaattg
     3001 caagcgcgtc cgaaagccgc atcttggccg agcctgcggc aacggctttt gctgactttc
     3061 atgcggggcc cagcccgata gaaagatcat ctcgaatgtt gcgtgcaggc gtccttcggg
     3121 atcggcaaac cgctgcgcat agttcaactg caacgccggc acctatcggc tggcgatggt
     3181 gcgccagccg tatcaggtga tcgacgacat gttcgcacgg ctggtgccga gcaacgaacc
     3241 ggagagccat tgcaatgatc tcaatcggga gacagcaatg gcggacaaac aaagacggtt
     3301 tacggatgat tttaagcgcg aggcaattcg tttagtggac agc
//
LOCUS       DATMPU010000351         2178 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_3998237,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000351 DATMPU010000000
VERSION     DATMPU010000351.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 2178)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2178)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2178
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3998237"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..293
                     /locus_tag="VNY06_08060"
     CDS             <1..293
                     /locus_tag="VNY06_08060"
                     /inference="COORDINATES: protein motif:HMM:NF021606.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2339 domain-containing protein"
                     /protein_id="HWX14780.1"
                     /translation="LAVALARMADGVRPQWFVVGARIEALVLVFLLVTLETRRFFETD
                     LIGWRYPASEAENYAYSAVWLAPGIVLLAFGLWRASREVCAWLRGSSSSRPF"
     gene            251..421
                     /locus_tag="VNY06_08065"
     CDS             251..421
                     /locus_tag="VNY06_08065"
                     /inference="COORDINATES: protein motif:HMM:NF021606.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2339 domain-containing protein"
                     /protein_id="HWX14781.1"
                     /translation="MRLASRLFVVAAVLKVFLYDLAQLEGILRALSFIGLGAVLIGIG
                     LAYQKLVFHRSM"
     gene            complement(676..1689)
                     /locus_tag="VNY06_08070"
     CDS             complement(676..1689)
                     /locus_tag="VNY06_08070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019401432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HWX14782.1"
                     /translation="MLKRAVLALAAILVLAVTAARADTLHQVKKRGYLACGSILGRAG
                     FGLPDGHGHWAGLDIDFCRALAAAIFDDAGKVKFVTLTAKDRFAALQSGAVDVLASNT
                     TWTLSRETKQGLLFVGINYFDGQRFMVHKNLNRASVLELSGTSICVQQFTTTELNLAD
                     FFHANRMTYKPVAVATADEAIEAYVSGRCDVYTADASGLYASRMALADPDDNIVLPEI
                     ISKEPLGPVVRQGDDRWFNVVKWVNFAMIIAEELGVTSKNVDEKATSENPEIRRLLGF
                     EGNFGKAMGLDPQWAYRIVKLVGNYGEIFDRNLGEGSPLKIKRGMNALWSKGGLLFAP
                     PIQ"
BASE COUNT          462 a          647 c          588 g          481 t
ORIGIN      
        1 tactcgccgt cgctttggcg cggatggcgg atggcgtgcg gccgcaatgg ttcgtcgtcg
       61 gcgcccggat cgaggcgctc gttctcgtct tcctgctcgt gacgctggaa acgcggcgtt
      121 tcttcgaaac tgatttgatc ggctggaggt atcccgcatc ggaagccgag aactacgcct
      181 attcggcggt ctggctcgct cccgggattg tgctcttggc ctttgggctc tggcgggcct
      241 cgcgcgaggt gtgcgcctgg cttcgcggct cttcgtcgtc gcggccgttc taaaggtatt
      301 cctctacgat ctcgcgcaac tcgaaggcat tctgcgggct ttgtccttca tcggcctcgg
      361 agccgtcctg atcggcattg gccttgccta tcaaaagcta gttttccacc gctcaatgtg
      421 agccaggcct gctaacgtca ctgaactcga agccgcgatc cagggatgga ttgagcatct
      481 caaccaagac tccgagcttt tcacttggac agccaacgcc aaccacatcc tcgccccatc
      541 gccgagccca aaaagccctg gccatcgtca aagcaagata caaatgaatg agccggaaca
      601 ctattaatag ttacctccgc cctcgctgtg ctgttcaggc gattttggga cccgcttgat
      661 aagccaagat cctgcttact ggattggcgg agcaaaaaga agcccacctt tcgaccacaa
      721 cgcgttcatg ccgcgcttga tcttcaatgg cgagccttca ccgagattgc ggtcgaagat
      781 ctcgccataa ttgccgacga gcttgacgat gcgatacgcc cattgtgggt cgagtcccat
      841 cgctttgccg aagttgcctt caaatccgag cagacgcctg atctccgggt tttcggatgt
      901 cgccttttca tcgacgttct tggacgtcac gcccaattct tccgcaatga tcatcgcgaa
      961 gttgacccat ttcacgacat tgaaccaacg atcgtcgcct tgccgcacga ccggccctag
     1021 tggttccttg gagatgattt ccgggaggac gatattgtca tccgggtcgg cgagcgccat
     1081 acgactcgcg taaaggcccg aggcatcggc cgtatagacg tcgcaacgtc cgctgacata
     1141 tgcctcgatg gcttcatccg cagtggcgac ggcgaccggc ttatatgtca ttcggttcgc
     1201 gtgaaaaaaa tcagcgagat ttagctcggt tgtcgtgaat tgctggacgc aaatcgaggt
     1261 gccggaaagt tcgaggaccg aagcacgatt gaggttcttg tgcaccataa aacgctgacc
     1321 gtcgaaataa ttgataccga caaagagcaa gccctgtttc gtctcccgcg atagggtcca
     1381 tgtggtattg ctggccagaa catcgaccgc gccggactgc aaggccgcga accggtcctt
     1441 ggccgtgagg gtaacgaact tcaccttgcc tgcatcatcg aagatcgcgg cggcgagcgc
     1501 gcggcaaaag tcgatgtcga gtccggccca atggccatgt ccgtccggca aaccgaagcc
     1561 agcacgccca agaatcgatc cgcacgccaa atatccccgt ttcttgacct ggtggagtgt
     1621 gtccgcccgc gcagccgtga cggcaaggac gagaattgca gcgagcgcta agacagccct
     1681 tttgagcatg tgagaggctt ccagaatgta ccgcaccgat gatcggtcaa gactgcgcgg
     1741 cggcgcatca gaatctcgcc ggcctttttt gcgaggactc ctacccgtag gctcatcgct
     1801 tcagtgacat gtaatcccgg cgctgcgtcc ggagcaatgc aacttagaat taaaagccat
     1861 tcccacccgc gatgaagaaa ggcgactcac ccggcgggca ataccctgca agacgcagca
     1921 aattccctgc tccgcttaat aaattccctg ttccaatttc taggggaatt atcctctaac
     1981 aagctgattt tgctgcattc tgtcgggcaa attctcgcgc agcagcccaa tttttgtaaa
     2041 attcccggta tccggtatta tggcgcaact gcctgaggca agggcgcgtc gatgccactt
     2101 aagcagcaac ccaattcggc atcgcgcgct tcggtctaca ggctgctaga ggcaaattaa
     2161 cgccgaggcc agcctaac
//
LOCUS       DATMPU010000352         3864 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_910967,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000352 DATMPU010000000
VERSION     DATMPU010000352.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 3864)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3864)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3864
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_910967"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..290)
                     /locus_tag="VNY06_08075"
     CDS             complement(<1..290)
                     /locus_tag="VNY06_08075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX14783.1"
                     /translation="MDAFEVVYTRIRRMTHKYVEGQVRFSYDNQWLIRALRLRSWRTR
                     RVNRRAGRAHRHTGLFPGRAEAAVGGFRQNSGQVATGWMDGSLVRRTTTFVSV"
     gene            complement(1201..1473)
                     /locus_tag="VNY06_08080"
     CDS             complement(1201..1473)
                     /locus_tag="VNY06_08080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX14784.1"
                     /translation="MRDTPDEASRDHHGADDSGGDGQLGYRMIADDALGAVPSVGRNG
                     GACGFRHDHHRNRTAFATVSERNLELCSRRVRFGGVARSECFDETH"
     gene            1837..2061
                     /locus_tag="VNY06_08085"
     CDS             1837..2061
                     /locus_tag="VNY06_08085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010911081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX14785.1"
                     /translation="MMNFTIEFFRTRPIDEAHATLDRISIVADNLDAASVKAKALFDT
                     LEMPQKPDGLRILDHVKRELFVWKPIDDDA"
     gene            2146..3129
                     /locus_tag="VNY06_08090"
     CDS             2146..3129
                     /locus_tag="VNY06_08090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011155942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HWX14786.1"
                     /translation="MQAMVLKKLGSALEWTDLADRPPGPGEIRVKVAACGVCRTDLHV
                     VDGELPNPKVPIIPGHEIVGRIDAIGAGVEGLSLGERVGIPWLGHTCGVCPYCMGRQE
                     NLCDRPLFTGYTRDGGFATATIAGARYAFPLGEVGSDVALAPLLCAGLIGWRSLVIAG
                     EGKKLGLYGFGAAAHIVAQVAKWQGRSVFAFTRPGDVATQAFARRLGTIWAGGSDEMP
                     PEPLDVAIIYATIGNLVPLALKAVRKGGRVVCAGIHMSDIPSFPYRLLWEERQLVSVA
                     NLTRQDGIDFLRLAPEIGIVTETTPYPLNQANRALADLRAGRFEGAAVLLP"
     gene            complement(3274..3471)
                     /locus_tag="VNY06_08095"
     CDS             complement(3274..3471)
                     /locus_tag="VNY06_08095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX14787.1"
                     /translation="MMKERQAIGIAKARRKATARAKTADVIELAKTGLVALTCPPNTA
                     LSRAARLLGARHSATFALSST"
BASE COUNT          818 a         1124 c         1085 g          837 t
ORIGIN      
        1 acgctcacga aagttgtcgt cctcctaact aacgacccat ccatccagcc ggtggcaacc
       61 tgccccgagt tctgacggaa gccgccgacg gccgcttccg ctcgtccggg aaatagtccg
      121 gtatggcgat gcgctcgtcc cgcccttcga ttcacccgcc gggtgcgcca tgaccgcagc
      181 ctcaatgcgc gaataagcca ctgattatca tagctgaatc gcacctgacc ttcgacatat
      241 ttatgcgtca tccggcgaat gcgggtataa acaacctcga aggcatccat cgcggcttaa
      301 atctcgcagc aaagattgcc accgctctca ctagagccgg tctcaaaaaa aaagacgaag
      361 ttaacgggga tcgagtaaac ggcggcggct cgagcgtgcg gctacgaaca aggtaattgt
      421 tttataaaac tgaaataaaa actgcgctaa tccatatgaa attcttcgga ttctaagaaa
      481 ttaataaatt ctaattgcat tttaagttgg aatatacgat tattaaaaag ccattaactt
      541 ggccctgcgc agaagaatgt ttgcgccaag ctgacaatct tcttacctga aaatacgatg
      601 atttatgttc tcgccggtgg cttggcgcta gccggcgtcg cattttctct agcgcggcta
      661 caggttcgcc tctcggtcaa cgcacttcga tcatggttcc gatttttggg tggcagttcc
      721 ggttgcgatg gccgttttag cccatctgaa agaagggaca agggaatgca gactgaaatt
      781 tctccctaca gtgcgacggg atatagagac gcgcttgcgc gtttggcgct gcaacgtgcc
      841 aacatcatgc atctagtgga cctgaaacgc gggggatatt ccccgcgtcg tacggaaatg
      901 cgaatcggcc gcctcagcgc ttatccaaaa gtgttgcccc catcgctcat actggcgccg
      961 cgaactttag tgaaagcatt cgccgatgac cgatgttgac gcttgcgttg tcccgtttca
     1021 atgtccgaaa tgcgggcatc tcttggaaca gacgataggt aagttaaaga ctcaagatca
     1081 catgctctgt cccggatgcg gtgtaggaat aaacattgac acgaataggt tatctaacgc
     1141 tgccaatgaa attcggaagg ctgtcgataa ggttccgccg gagatcacgg ttaagttcta
     1201 ttaatgcgtt tcgtcaaagc attccgaacg tgctacaccg ccaaagcgaa cgcggcgcga
     1261 gcaaagttcg agattgcgct cgctcaccgt cgcaaatgcg gtgcggtttc tatgatggtc
     1321 gtgtcgaaac ccgcaagcac cgccattcct gccaacgctt gggactgccc ccagggcatc
     1381 atcggcgatc atgcgatatc caagttggcc atctccgccg ctgtcgtcgg cgccgtggtg
     1441 atcgcgactc gcttcgtctg gagtatcccg caacttatct tccgcgctgg ctctttcccc
     1501 cactcaagcg cagggatcgg cgaaacggaa gcccatggcc aactaagagc gcggtctttt
     1561 agaaaaggcg cgcgctcatt ttttcaatcg ctgctgtggc cttgcttata agccggttga
     1621 tcagtgcatt cacttgaccg cgcgacttaa cattcccacg agtatgtcgg cgatcatcgc
     1681 cacaagcacc gccatcaaag gattgcgctc gattgttgtc tcgagctccg tgctcgcgcc
     1741 agcgacctaa gtcgcagcat tgcctatggt caactagata gacacctaac cctcccgttg
     1801 ccgtggtaca cttggtataa agaagtgagc agtctcatga tgaatttcac catcgagttt
     1861 tttcgcacca ggccgattga cgaggcgcac gcgactttgg atcggatttc gatagtcgct
     1921 gacaatctcg acgctgcatc ggtgaaagcg aaggcgctct tcgatacgct tgagatgcca
     1981 caaaaaccgg atgggttgcg catcttggac cacgtaaaac gagaattgtt cgtttggaag
     2041 ccgattgacg atgatgctta aatgcaagag gcaagaagcg agcctcgtga ccgttgctgc
     2101 ggacaatgcg gagcggccgt catcgaggtg gcctaggaga acgccatgca ggcgatggtg
     2161 ctgaagaagc ttggctctgc tcttgaatgg acagatctcg ccgatcggcc acccgggccg
     2221 ggtgagattc gggtgaaggt ggccgcctgc ggcgtctgcc gcacggacct gcatgtcgtc
     2281 gatggcgagc tgcccaaccc gaaggtgccg atcatccctg gccatgagat cgtcggccgg
     2341 atcgatgcga tcggggccgg cgtcgaaggg ctcagcctgg gggagcgtgt gggcatccca
     2401 tggctcggcc acacctgcgg cgtctgcccc tactgcatgg ggcgccagga aaatctgtgc
     2461 gatcgtccac ttttcaccgg gtatacacgc gacggcggct tcgcgacggc gacgatcgcc
     2521 ggtgcgcgct atgcgttccc gctcggcgag gtcggcagtg acgtggcgct agcccctttg
     2581 ctctgcgccg gtctgatcgg ctggcgttcg ctggtgatcg ccggtgaagg caaaaagctg
     2641 ggactctacg gctttggcgc cgcagcccat atcgtggcgc aggtcgcgaa atggcagggg
     2701 cgatcggtgt ttgccttcac gcggccagga gatgttgcca cccaggcctt cgcccggcgc
     2761 ctcggcacga tatgggctgg cggatccgac gagatgccgc cggagccgct cgatgttgcg
     2821 atcatctatg cgaccattgg caatctcgtg cccctggcgt tgaaggcggt ccgcaagggt
     2881 ggtcgcgtcg tttgcgcggg catccatatg agcgacatcc cgagcttccc ctatcggctg
     2941 ctatgggaag agcggcagct cgtgtcggtc gccaatctca cgcggcagga tggaattgat
     3001 ttcctgcgcc tggcgccaga gatcggcatc gtcaccgaga ccacgcccta tccgctcaac
     3061 caggccaacc gggccctcgc cgatctccgc gccggccggt tcgaaggcgc cgcagttctc
     3121 ctcccctgag cacggttacg cccatgtcgt tcggcattgc ccagagccgc tttcttaata
     3181 atgctacccg caaacttcga cttctcaccg tcaccacttc tggaccgaaa taagggctcg
     3241 cgacttccgc ttcgggaaaa agccgacact cgttcaagtt gacgaaaggg cgaaggtagc
     3301 tgaatggcgc gcgccgagca gacgggctgc tcgggaaaga gctgtattcg gcgggcaggt
     3361 cagggcaacg agtccagtct ttgccaattc gatgacgtcc gccgtcttgg cgcgcgcggt
     3421 cgccttcctg cgcgcctttg cgatgcctat ggcttgccgt tccttcatca tcgacaattc
     3481 aaattgctcg atttagccaa gaaggtttac catcaacgca ccggttggcg tcgaagtatc
     3541 acgcgccatg tcgagaatgc gcaaatgaac gcctttcgcc tccagcgtcg tgactagcgc
     3601 aaagatggga gatgcttctc gcgagacgat caagctttac cacgcacaag cggcgtcaag
     3661 ctgcggacgg tcgccaacgg cggataccac ttcagaaaag atttcctcgc atcaatcccg
     3721 ccgccgcaat ggagcgtacc ggcacaatct cctggcttag ccagtgtgcc tctatacatc
     3781 cgccagggtg ctcggggctg ggaggcgctt tatgccctgg ccttcaactg ctactccatg
     3841 ccctggccaa tcctgaccca gccg
//
LOCUS       DATMPU010000353         3098 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_578894,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000353 DATMPU010000000
VERSION     DATMPU010000353.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 3098)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3098)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3098
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_578894"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(71..589)
                     /locus_tag="VNY06_08100"
     CDS             complement(71..589)
                     /locus_tag="VNY06_08100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011424584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HWX14788.1"
                     /translation="MTIRDLIPWTRSQDLVPELFRDERSSPFLTLHREMNRLLDEAFR
                     GFQMSPFAGQRTDFVNPAWPKLEVAETDKKITVSAEIPGMEEKDIEVVFNDGNLILRG
                     ETKSETEDKERHFSERFYGRFERRIPVGMDIEEDKIQASFKNGVLKIILPKSERAQTK
                     EKRIAINAPTKH"
     gene            complement(841..1794)
                     /gene="coaA"
                     /locus_tag="VNY06_08105"
     CDS             complement(841..1794)
                     /gene="coaA"
                     /locus_tag="VNY06_08105"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HWX14789.1"
                     /translation="MDERAPLMLSPYHRFTRDEWAALRADTPLTLSAEDLARLKSLND
                     PISLEEVVAIYLPVSRLLALYVAATQGLYKATQRFLGAEGGKVPYIIGIGGSVAAGKS
                     TLSRVMQALLQRWPNTPKVELITTDGFLYPNTVLQRQGLLEKKGFPESYDSIKLLSFV
                     SDVKAGKRDVRAPVYSHVTYDIVPDESIVIDRPDILILEGLNVLLPNRGAEDGRTSPF
                     VSDFFDFSVYLHASEEDLEKWFVARFMRLRDTAFRDPSSYFKKYASLDDMGAELTARA
                     IWRQINLPNLRENILPTMPRASLVLTKGPNHTIEEVELRKL"
     gene            complement(1795..2136)
                     /locus_tag="VNY06_08110"
     CDS             complement(1795..2136)
                     /locus_tag="VNY06_08110"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009490711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-ATP diphosphatase"
                     /protein_id="HWX14790.1"
                     /translation="MTSFTLDDLAAIIEARADASASVSYTKSLLDAGPARAAKKLGEE
                     AVEVVIAAVEGDVEALTRESADLLYHLLVILHQKGITLADICAELEHRTGRSGYAEKA
                     ARQADAAPAED"
     gene            complement(2133..2972)
                     /gene="hisF"
                     /locus_tag="VNY06_08115"
     CDS             complement(2133..2972)
                     /gene="hisF"
                     /locus_tag="VNY06_08115"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012383257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HWX14791.1"
                     /translation="MLKCRVIPCLDVKDGRVVKGVNFVNLRDAGDPVQCAIAYDAAGA
                     DELCFLDITASHERRAIMLDVVQRTAEACFMPLTVGGGTRTLDDVRALLLAGADKVSI
                     NTAAVEKRAIVRDAAQKFGSQCIVVAIDAKQMSPGKWEIFTHGGRNATGLDAIAYAKE
                     VVGLGAGEILLTSVDRDGTKSGFDLALTRAIAEAVPVPVIASGGVGTLNHLVEGIRDG
                     GASAVLAASIFHFGEHTIAQAKQHMANSGLPVRLDFIRGAARATPRSCVFENEAASMD
                     HAP"
BASE COUNT          657 a          871 c          883 g          687 t
ORIGIN      
        1 aacgcttgcc cgctgatgcg agtccagacg gttccgccgg gcaacggtgc aaccgtctga
       61 ttgtaagcga tcagtgctta gtcggggcgt tgatggcgat ccgtttttcc tttgtctgcg
      121 cccgttcgct tttcggtagg ataattttta gcacgccgtt tttgaacgag gcttggatct
      181 tgtcttcctc aatgtccatg ccgacgggaa ttcgccgctc gaaccggcca tagaaacgtt
      241 ctgagaagtg gcgttccttg tcttccgttt cggatttcgt ttcgccgcga agaattaaat
      301 tgccgtcgtt gaagacaacc tcgatgtctt tttcttccat tccgggaatt tcggcggaaa
      361 cggtaatctt cttgtccgtt tcggcgactt cgagcttcgg ccaggcagga tttacaaaat
      421 cggttcgctg cccggcaaat ggagacatct ggaagccgcg aaaggcttcg tccagtagcc
      481 ggttcatttc gcggtgcaat gtgagaaacg ggctagatct ttcatcgcgg aaaagctcgg
      541 gaacgagatc ttggctccgg gtccatggaa tgagatcacg tatagtcata gcttaatctc
      601 caaggtctga gtggtgcatc gcgcgcgaat tccgcccccg ctgctcacga cccgcgacat
      661 ggcggtgatg cgcgcataaa aatctggagc gcggctcgcg tcattcaaga ggcgaattgc
      721 acgtgttgcc ggccagatat ccatcgggat gccgctcgat cgaaaggcta tatgttcgtg
      781 ttgtacaaga tcacataata agttggttat tggcaatatg agtagagggc agcggggggg
      841 ttaaagtttc ctaagctcga cttcctcaat ggtatgattt ggccctttgg tcagcacgag
      901 gctcgcgcgc ggcatcgtcg gcaaaatatt ttcgcgcaga ttgggaagat tgatctgtcg
      961 ccagatcgca cgtgcggtca attccgcgcc catatcgtcg agactcgcgt atttcttgaa
     1021 ataggacgaa ggatcgcgaa acgcggtgtc gcgcaggcgc atgaagcggg cgacaaacca
     1081 tttttcgaga tcttcttccg aggcgtgcag atagaccgaa aagtcgaaga aatcggaaac
     1141 aaacggacta gtgcggccat cctccgcccc ccgattgggc aaaagaacat tgagtccctc
     1201 gaggatgaga atgtccggac gatcaatgac gatgctttcg tcgggcacga tgtcgtaggt
     1261 cacatgcgaa taaacgggcg ctcgcacatc acgcttgccg gctttcacat cggacacaaa
     1321 ggacaagagc tttatgctgt catagctctc gggaaaccct tttttctcca acagaccctg
     1381 gcgttgcaga accgtgttcg gataaaggaa tccatcggtc gtgatgagtt cgacttttgg
     1441 cgtattcggc caacgctgca aaagcgcttg catgacgcgc gacagcgtcg attttccggc
     1501 tgcgaccgag cctccaatgc caataatata gggtactttt ccgccctcgg cgcctaaaaa
     1561 gcgctgggtc gctttgtaga gcccctgcgt cgctgcaacg taaagcgcga gcaggcgcga
     1621 caccggcaga tagatcgcga cgacttcttc caatgatatc ggatcattga gcgatttcag
     1681 ccgcgcgaga tcctctgccg ataaagtgag cggtgtgtcg gcacgtagtg cggcccattc
     1741 atcccttgtg aaacgatggt agggcgacag catcaggggc gcgcgttcgt ccatctagtc
     1801 ctcggccgga gcggcatcgg cctggcgagc ggctttttcc gcatagcctg accggccagt
     1861 acgatgttcg agttccgcgc agatgtcggc taatgtgatg cccttctgat gcaagatgac
     1921 aagcagatga tagagaagat cggcgctttc gcgggtcagt gcctcaacat cgccttcgac
     1981 cgcggcgatc acgacctcga ccgcctcctc gccgagtttc ttcgctgctc gggccggtcc
     2041 tgcgtcaaga agcgatttcg tgtaagaaac cgaggcgctc gcatcggccc gcgcctctat
     2101 gatcgcagca agatcatcca gggtgaagct tgtcatgggg cgtggtccat actggcggcc
     2161 tcattttcaa agacgcaact ccgcggcgtg gcgcgcgccg cgccacggat gaaatcgagg
     2221 cgcacgggca gcccagaatt tgccatgtgc tgcttggctt gcgcgatcgt atgttcgcca
     2281 aaatgaaaaa tcgaggcggc aagcaccgcg ctcgcgccac cgtcgcgaat tccctcaaca
     2341 agatgattga gagttccaac gcccccggaa gcaatcacgg ggaccgggac ggcttcggcg
     2401 atggcccttg tcagcgcgag atcaaagccg ctcttggtgc cgtcgcgatc caccgaggtc
     2461 aacaggattt cgcccgcccc gagaccaacc acttctttgg cgtaggcgat ggcgtcaaga
     2521 cccgtcgcat tgcgcccgcc atgagtaaaa atttcccatt tgccggggct catttgcttg
     2581 gcgtcgatcg ccaccacaat acattgactg ccgaatttct gtgcggcgtc acggacgata
     2641 gccctttttt ccacggctgc cgtgttgatc gatactttgt cagcgccggc aaggaggagt
     2701 gctcttacgt catctaaggt gcgtgttccg ccgccgacgg tgagcggcat gaaacaggct
     2761 tccgccgtgc gctgaacaac atcaagcatg atcgcgcggc gttcgtggct tgcggtgata
     2821 tcgaggaaac atagctcgtc ggcgcccgcc gcgtcataag cgatcgcgca ttgaaccgga
     2881 tcgccagcat cgcgcaaatt gacgaaattg acgcccttga cgacccggcc atccttcacg
     2941 tccagacagg gaatgacacg gcatttaagc atctcgcagg cccccggcgg aaaatattaa
     3001 cttcatgcca ccctcccgcc ggacgagcga cagggcgttt gcggcatcga gccgtccgtc
     3061 ataaagcgcc cggccggcaa tggccccttc aagtttgg
//
LOCUS       DATMPU010000354         3047 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_2157660,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000354 DATMPU010000000
VERSION     DATMPU010000354.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 3047)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3047)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3047
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2157660"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            616..912
                     /locus_tag="VNY06_08120"
     CDS             616..912
                     /locus_tag="VNY06_08120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX14792.1"
                     /translation="MSFFEEVDHLLRGPLDLVPLANASVYSLGFLIFGVPVWKCAVVG
                     IVAFAASKLHYGRRALSRLGVLIMIASTFVWAGLTPDTQDLVRTARLVVAEFHP"
     gene            complement(969..1127)
                     /locus_tag="VNY06_08125"
                     /pseudo
     CDS             complement(969..1127)
                     /locus_tag="VNY06_08125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018317101.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
     gene            2235..2570
                     /locus_tag="VNY06_08130"
     CDS             2235..2570
                     /locus_tag="VNY06_08130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX14793.1"
                     /translation="MASKRTEIAIEKIGAACDGDMRGALEALLLVNMHPDSLPGRAVR
                     RERWNAHVQIARNEWRKASAIEIDRLTVEREIQALIRLVIFLRIISMCSGVFVAAERM
                     ELRLALLAR"
     gene            2851..>3047
                     /locus_tag="VNY06_08135"
     CDS             2851..>3047
                     /locus_tag="VNY06_08135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX14794.1"
                     /translation="MPDQMRHVRFTPDPGSYGAPGEPVSYSEITHKSAKDAADCSVRR
                     HSARRACSAVNASRDRGGDLLL"
BASE COUNT          615 a          837 c          888 g          707 t
ORIGIN      
        1 cggacgcggg tgagatcaag cacggtgaag cgggacaggg cctgcgaggc actcgggaat
       61 ggcatttaag gggctccgga caggaaaaag gcaaactgag atatcccctt gatcaatgga
      121 atctctcata agatcaatag cgatcaggga ctggccatcg acgtaaggcg cttgttcggc
      181 caaacaggcg tcggactgcg cgatcagcct gcgggccgag atcgcggtgc aaccgcgtcc
      241 atgggtatcg cctagggcaa ctggaacgcg ggatacgagg gggaacaggc ttgtacgcgc
      301 gcctcgcatc agtagctcgc tcccggacga taggacgaac ctgcgcttgg cgcgcttctg
      361 gcctgcggtc gtaggccgcg gttaggccgc tccgaaccgg gtcgctctcg gttcctaagc
      421 ttgcacggcg ccaatatttg cccgaggtcg cgccaggggt ggggcaatcg tcctagatgt
      481 agagttcgct gcgaccgtgc ccaaatgggc gcataaactg ggcagcctgg aacctttctg
      541 ttcaaaacaa aaccattttg ttggtgtgcg atagtcgctt tccggattct tttcctcgca
      601 ggaggggttt gggcgatgtc cttttttgag gaagtcgatc atttgcttag gggcccgctc
      661 gatctggtcc cgctcgcgaa tgcctcggtg tattcgttgg gttttttgat tttcggggtg
      721 cccgtttgga aatgtgccgt tgtcggcatt gtcgcgttcg ccgcttcgaa acttcattac
      781 ggccgccgcg cgctttcccg cctcggtgtg ttaatcatga tcgcgtctac gttcgtttgg
      841 gcggggttga cgccagatac gcaagatttg gtgcggacag ctcgattggt cgtggctgag
      901 tttcacccat agcccgaacc ctaaaactgc gagagagatc aaccggcctc cagcgtgaat
      961 agcgcttagc gtcgtgcaca ggtgagggcg gctcccatcc cacgcggcgc cgccgtttcg
     1021 ctgaccgcac agaccggcca gtccaatgac acccattcac ctgatgaatg agccagggtt
     1081 gtggtgagtc ggatcagccc agccgcttcg tcgatcgagg tagtcatccc gctttgactg
     1141 taggggttcg gtcgcccgct cgcaagttct gggcagggcg cccaattggc cacaactctc
     1201 ggtctcggcg acaagccaag ctagtggcgc cccgccgacg ggtttcctgc agatcgctta
     1261 atcccttgcg ttccaacgaa aatccgattt gggttcgttc tgccaatttt gatcatagga
     1321 gcatgaagtt cgaaaggtgt cctcgaggcg ttatcaacta atgtgcttcc tcagactttg
     1381 ccgcacccaa ttcagctggg catagaagtg caaccacatg cctcgaaggc ctacgcccag
     1441 tttggagccg gtccacgtat gccctggtcg ctatctcgct gcggttgaaa taggcttgtg
     1501 ctgtcaatac tttcgcgcaa ttttctatgg agttagcaac gaggcctgaa tccattgctg
     1561 ctttcaccat gatgctgaac acgatgccag gacatctagt ccgagcgctc tagaacgaac
     1621 ttgtatgtcc gcatgcaaag gcatgacgaa atgccttcga gtcttttttc tgggaatgca
     1681 gacatgggca gggcggcgag ccgtgtgatg gtcgcgaagt ccaagtcctt ttcctgcctt
     1741 aatcaggttt accgcaagat caaatttcct gcggctcagg cggcttcttt tgttcaataa
     1801 ccgcgctgac gtcgggatcg aatgggcctg ccatctggga ctggtcaact gcaacgatct
     1861 ggttttgaaa ttctttcatt gcctgtaggt cagtggcgtg gtctcgcgcc gttgcgctga
     1921 gaccctgctt ttcaagccag cgaagggcga tagccagctg ccttagatca ttccgatggg
     1981 gaccgactgg tagcttgcgc gctcccggag cgcatctgtg gcttgctttc tgatcggtga
     2041 aacgaccgta tttgacggac gcttcatctg tctaccttgg gcggggccgt cggtggggcg
     2101 cgctggcaac aacaatttat ttaaaagact cgcagagtca ataatattta attgcattta
     2161 atttggattt atttacgcta attgaaactt taatcatttt caatcccaac gggtgattat
     2221 gcaggggatt aggcatggct tccaaacgga ctgaaattgc tatcgaaaag atcggcgcag
     2281 cttgcgatgg tgacatgcgc ggagcactgg aggctctgct actcgtcaat atgcaccccg
     2341 atagtctgcc cggtcgcgct gtgcgacgcg agcgctggaa tgctcatgtc cagatcgcgc
     2401 gcaatgaatg gcggaaagcc agcgcgattg aaatcgatcg attgacggtc gagcgcgaaa
     2461 tacaggcgct cattcgattg gtaatcttct tgcggatcat ctcgatgtgc tccggcgtgt
     2521 tcgtcgccgc agagcgaatg gaactaagac tcgcgctcct cgctcgataa gcaagccgcc
     2581 accggttccg ctgatcttgg atatgtgggc ctatggctgc acagccagca cgatcgcgca
     2641 cgcgctcggg aagagttaca agaccgttgc caacatcgtc ggtcgcgcaa ggtcggacgg
     2701 cccgcattta gccggcccca gagcctcagg gccacgcctt gtgcaatggt ccagactagc
     2761 actttgcggc cgggcggcga gtgagaaata gcaaacaacc ggcctcagaa gctggacagg
     2821 ccccatgtct cagttgggtc aaactgcgac ttgcccgacc aaatgcgcca tgtccgcttt
     2881 acccccgatc ccggaagcta tggcgcaccc ggcgaaccgg tttcctattc ggaaattacg
     2941 cacaaatcag cgaaagacgc tgcggattgc tcggtgcgcc gccattcggc acgacgtgca
     3001 tgctctgctg ttaacgcttc gcgcgatcgc ggtggcgatc tactgct
//
LOCUS       DATMPU010000355         4371 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_3378187,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000355 DATMPU010000000
VERSION     DATMPU010000355.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 4371)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4371)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4371
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3378187"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1011
                     /locus_tag="VNY06_08140"
     CDS             <1..1011
                     /locus_tag="VNY06_08140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012589385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HWX14795.1"
                     /translation="QYIGGIEHAILHLLYARFFTRAMQVAGHVGALREPFAGLFTQGM
                     VVHETYRAASGEWHYPADVRIEVDADRRRAFHIESGEEIAIGAIEKMSKSKKNTIDPD
                     DIISTYGADTARWFVLSDSPPERDVIWTEEGVQGAAKFVQRLWRLSGELAEIAAPAET
                     QLPKDFSPTAMDIRRAVHMSLIRIEEDLERLRFNRAVAQVHDLANKLSAAIGAVESEN
                     IAPEIRAAFREAADILVLMVSPMMPHLAEECWALLGHADLAAEQAWPIADRELVVEDT
                     IEIPVQVNGRKRADLVVRRDADKVAIEEAALALDPVRRALDGKPVKKIIIVPQRIVNV
                     VA"
     gene            998..1531
                     /gene="lptE"
                     /locus_tag="VNY06_08145"
     CDS             998..1531
                     /gene="lptE"
                     /locus_tag="VNY06_08145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012383238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HWX14796.1"
                     /translation="MSLPEHWMHGRAFVRALFVLGIGLALSGCFQPLYGSLAEGGDVA
                     GWLQTIKVDPIAGRTGHYLGNELIFALNGTGAQVPAKYHLAVTTTANVQTPLLDTVSG
                     YPTAGNVVVNAEYKLLPMGSDTPVTKGKVTTLESYDRTSQRFANLRAARDAEIRGAKM
                     LADQIRNLIAAALAKGG"
     gene            1537..2556
                     /gene="holA"
                     /locus_tag="VNY06_08150"
     CDS             1537..2556
                     /gene="holA"
                     /locus_tag="VNY06_08150"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012589388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HWX14797.1"
                     /translation="MVAIKSHEADRFLARAAPDIFLYFVFGSDAGLVAERTRKIISRT
                     VKDPKDPFQLVRINGDDLAADPLRLADEANTIPLFGGRAIWIEAQGKAFLSAIEPLLA
                     LPPRDCTIVIEAGALKKDAPLRTLCERAKGAAAIPCFPDNEREIAQLIDAEVRAANLS
                     IAPDAKEFLVTQLGQDRLSTRSELEKLVVYAHGAGEITLDHVAAIVCDASNLIVEETV
                     NRAYQGELAALDASLQHIFAGASDYPLLLATALRHALELHRARSGTDGESGSFRHPSR
                     PMGFKRREAFGQHLRIWKLASLAQAIDTLARAIAQARREPGLGSSLAARALWAIAFFA
                     RKNPP"
     gene            complement(2581..3159)
                     /locus_tag="VNY06_08155"
     CDS             complement(2581..3159)
                     /locus_tag="VNY06_08155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012589383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HWX14798.1"
                     /translation="MLTKPAARGQHVVKRGLSRLLATRMPRYGQATGKPVGRFHAGGV
                     VLPCALGRAGISHAKREGDQATPAGAFRLLQGFYRRDRLMRQAWPLPMAGIRPSDGWC
                     DDPDSGCYNRPVKLPCRATCEELWRRDHLYDLLVVLDYNIHPRCKSCGSAIFLHCARL
                     NFAPTEGCVALRFEDLRRLLPRLSRKAGLIIR"
     gene            3392..4078
                     /locus_tag="VNY06_08160"
     CDS             3392..4078
                     /locus_tag="VNY06_08160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012331332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HWX14799.1"
                     /translation="MTQVLKILIADDDPDLRAALAEQLALHEEFIAVEAGTARGALET
                     AREEMPDIIIMDVGLPDMDGRAAVRQLREEGFKNPIIMLTAHNSETDTVEGFDAGAND
                     YVAKPFRFAELLARIRAHLRQRESSEEALFRIGPYTFKPGAKHLIGEKGDKLRLTEKE
                     TAILRFLYRAGQAVIPREVLLREVWGYNSNVTTHTLETHIYRLRQKIERDPTKAQLLV
                     TAAGGYKLIP"
     gene            4128..>4371
                     /locus_tag="VNY06_08165"
     CDS             4128..>4371
                     /locus_tag="VNY06_08165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012589380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding protein"
                     /protein_id="HWX14800.1"
                     /translation="MGLDDDIGKLTRNPTFAVLEQEALRLIAFSAETKTLRAGDVLFR
                     RDEISNDGFVVLTGSIAVDPSGLGAATARIVRPPGLI"
BASE COUNT          915 a         1248 c         1315 g          893 t
ORIGIN      
        1 caatatatcg gcggcatcga acatgcgatt ttgcacttgc tctatgcaag attcttcacc
       61 cgggcaatgc aggtggcggg tcatgtcggc gcgttaagag agccttttgc cgggctcttt
      121 acccaaggca tggttgtcca cgagacctat cgtgcggcct ctggcgaatg gcattatccg
      181 gccgatgtca ggattgaggt tgacgcagat cgacgccgcg ctttccacat tgagagcggc
      241 gaagaaatcg caatcggcgc gattgaaaaa atgtcgaagt cgaagaaaaa caccatcgat
      301 cctgacgaca ttatttcgac ctatggcgcg gataccgcac ggtggtttgt gctgtccgat
      361 tccccgcccg agcgcgatgt catctggacc gaggaaggcg tgcaaggggc agcgaaattc
      421 gtgcagcgcc tgtggcggct ttcgggcgag ctcgcagaga ttgccgcgcc cgcggagacg
      481 caactgccca aagatttttc tcccaccgca atggacattc gcagggcggt tcacatgagc
      541 ctgatcagga ttgaggaaga tcttgagcgg ctgcggttca atcgcgctgt cgcgcaagtc
      601 cacgatctcg caaacaagct ttcggcggcg atcggtgctg tcgagagcga gaatatcgcg
      661 ccggaaatca gggcagcctt ccgtgaggcc gcagacattt tggtgctcat ggtttcgccg
      721 atgatgccgc atcttgcgga ggaatgctgg gcgcttctcg gccacgcgga tttggcggcc
      781 gagcaggcct ggcccatcgc cgacagggag cttgttgtcg aagacacgat cgaaattcca
      841 gtgcaggtca acgggcgcaa gcgggcggac ctcgttgtcc ggcgggatgc ggacaaggtg
      901 gcaatcgagg aagcggcgct cgccctcgat ccggtccgac gcgcgcttga cggcaaaccg
      961 gttaagaaaa tcatcattgt tccgcaaagg atcgtaaatg tcgttgcctg aacactggat
     1021 gcatggccgc gcctttgtgc gcgcgctctt tgtgcttggg ataggccttg ccctttccgg
     1081 ttgcttccag ccgctctatg gctcgctcgc agagggcggg gatgtcgcgg gctggttgca
     1141 gaccataaag gttgatccca tcgccggccg gaccggccat tatcttggca atgagctgat
     1201 cttcgctctc aacggcaccg gcgcgcaggt ccccgcaaaa taccatctgg cggtgacgac
     1261 caccgcaaac gtgcagactc ccttgctcga tacggtaagc ggttatccaa cggcgggaaa
     1321 tgttgtggtc aatgcggagt acaagctatt gccgatggga agcgacacgc ccgtcacgaa
     1381 gggaaaagtg acgacgcttg agagttacga tcggacgagt caaagatttg ccaatcttcg
     1441 tgccgcgcgc gacgcggaaa tacgtggcgc gaaaatgctc gccgatcaga tccggaacct
     1501 tatcgcagca gcgcttgcca agggcggata gctgacttgg tcgcgatcaa gagtcatgaa
     1561 gccgatcgat tccttgcccg cgcggcacca gatatttttc tctatttcgt ctttggatcc
     1621 gatgcaggac tcgtggccga gcgaacgcgg aaaatcattt cccgcaccgt caaagacccc
     1681 aaggatccat tccagcttgt gcggatcaac ggcgatgacc ttgccgccga ccctttgcgc
     1741 cttgccgacg aagccaatac cattccattg tttggcggcc gggcgatttg gattgaagcc
     1801 caaggcaagg cgtttctctc cgcgatcgag ccgcttcttg ctttgcctcc gcgcgattgc
     1861 accattgtca tcgaagccgg agcgctgaag aaggatgcgc ccttacggac gctttgcgag
     1921 cgcgcgaaag gcgccgccgc aatcccatgc tttcccgaca atgaaaggga gatcgcgcaa
     1981 ttgatcgatg cggaagttcg cgccgcaaac ctgtcaattg ctccggatgc gaaggaattt
     2041 ctcgtcacgc agctcggcca ggaccggctc tcgacgcgct ccgagctgga gaagctcgtc
     2101 gtttacgccc atggcgcggg cgaaatcacg ctcgatcatg tcgccgctat tgtctgcgac
     2161 gcatcgaacc tcattgtcga ggaaacggtg aatagggcgt atcagggtga actcgcggcg
     2221 ctggacgcca gtctgcaaca tatcttcgcc ggggccagtg actatcccct gcttcttgct
     2281 acggcgctgc gtcacgcgct ggaactgcat cgtgcgcgct ccggcacgga tggggagtct
     2341 ggatcgtttc gccatccttc ccgcccaatg gggttcaagc gaagggaagc gttcgggcag
     2401 catctgcgca tctggaagct ggcttcgctt gcccaagcaa tcgacacgct tgctcgggcg
     2461 atcgctcaag caaggcggga gcccgggctc ggatcctccc tggctgcccg ggcgctctgg
     2521 gcaatagctt tttttgcccg aaaaaatccg ccatgagctg ggatgaaggc gagcctgctt
     2581 ttacctgatg atgagcccgg cctttcggga aagccgcggc agcaaccggc gaagatcctc
     2641 gaatcgcagc gcgacgcacc cctccgtcgg cgcgaaattg agccgcgcac aatgcagaaa
     2701 aatcgcgctg ccgcaagatt tgcagcgcgg atggatattg tagtcgagca cgaccaggag
     2761 atcgtagaga tgatcccgcc gccataattc ttcgcaggtt gcgcggcatg gcagctttac
     2821 gggccggttg tagcacccgc tatccggatc gtcgcaccat ccatcgctcg gccgaattcc
     2881 ggccatcggc aatggccaag cttggcgcat cagccgatcc ctgcggtaaa agccctgaag
     2941 aaggcgaaag gctccggcag gtgttgcctg atcgccttcc cgcttcgcgt ggctgatccc
     3001 cgcccgcccg agggcgcagg gaagaacaac accgccggcg tgaaatctac ctacaggttt
     3061 tcctgtagcc tggccgtagc gcggcatgcg cgttgctaga agccggctca ggccgcgctt
     3121 aaccacatgc tggcctcgcg ccgcgggttt tgtcagcatt ttgcaagaaa attcgtgaaa
     3181 ccttatagtt tggagcgatc aatcgcgatt cgcagcgcga taagcgcgta atcagtcttg
     3241 cccatttttg tgccacagta aaacattaac ccggtcgacc agaaccgagc gaacaaaagg
     3301 gaaaggggga gcgccgcgcg gtcaaaggaa ccggattgat tccgtggtag ttatcgaacg
     3361 gaatgtcggc aggttcgtca aggacgagta catgacccag gttctaaaaa tactgattgc
     3421 cgatgatgac ccggatttgc gggctgcctt ggccgagcag cttgccctgc atgaagaatt
     3481 tatcgccgtc gaggcgggaa cggcgcgtgg tgcgctcgag actgcgcgtg aagagatgcc
     3541 cgacatcatt attatggatg ttgggctccc ggatatggat ggacgggcag ccgtcaggca
     3601 gttgcgcgag gaagggttca agaacccgat cattatgttg accgcacata attcggaaac
     3661 cgacaccgtc gaaggatttg acgccggggc caatgattat gtggcaaagc ccttccgctt
     3721 tgctgagctg cttgcacgca ttcgcgccca tttgcggcag cgcgagtcaa gcgaggaagc
     3781 tctgtttcgg atcggccctt acacattcaa gccgggcgcc aaacatctca ttggagagaa
     3841 gggcgataag ctgcggctga ccgaaaagga aaccgccatt ttgcgtttcc tttaccgcgc
     3901 cgggcaggcg gtcattccac gcgaggtgct gctgcgcgaa gtctggggct ataattcaaa
     3961 tgtcactacg cacacgctcg aaacgcatat ttaccgtctg cgacaaaaga tcgaacgcga
     4021 tcctacaaag gcgcagctac ttgtaactgc ggcgggaggc tataagctga tcccttaatg
     4081 gcgcagcaaa ttcttgccgg aagtaaagtc tcgcgggtcg cgctggcatg ggactagacg
     4141 acgatatcgg taagcttacg cgcaatccga catttgccgt gctggagcag gaagcgctgc
     4201 ggcttattgc tttttccgcc gaaacaaaga cgcttcgcgc aggcgatgtt ctattccgcc
     4261 gcgacgaaat ctccaatgac ggttttgtcg tgcttaccgg gtcgatcgcc gtcgatccgt
     4321 ccggcctcgg ggccgccacg gcgcggattg tccggccccc tgggctcatc g
//
LOCUS       DATMPU010000356         2865 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_3378715,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000356 DATMPU010000000
VERSION     DATMPU010000356.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 2865)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2865)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2865
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3378715"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..615)
                     /locus_tag="VNY06_08170"
     CDS             complement(<1..615)
                     /locus_tag="VNY06_08170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003609284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HWX14801.1"
                     /translation="MTISAAIHHITHYKYDRPVVLEPQTIRLRPAPHCRTKIPAYSLK
                     VTPAEHFVNWQQDPHGNWQARFVFPEKVTEFKIEVDLTAELAVINPFDFFIEPYAEQF
                     PFAYDPELKAQLAPYLDAETLGPRLAAYIAGLPKDPIRVVDFLVDLNTALRKAIRYLI
                     RMEPGVQTPEETLSLGSGSCRDSAWALVQILRHLGLAARFVSGYL"
     gene            complement(974..2083)
                     /locus_tag="VNY06_08175"
     CDS             complement(974..2083)
                     /locus_tag="VNY06_08175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007879666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine ABC transporter substrate-binding
                     protein"
                     /protein_id="HWX14802.1"
                     /translation="MKNSFAALWLFGLCLCGPLLPAFGQEKAVSIYGWGDYIDPKVIE
                     DFTKETGIKVTYDGYDSNEAFEARFLAGKTGFDVVIVPGPVLQRQIAAGVYQKLDKTK
                     LPNSKNLWPEVMARLAVYDPGNQYAVNYMWFTAGIAYNVEKAKELLGDAAADASLNSW
                     DILFKPRNLKKFAGCGVTVLDSGEDLFAIALNYLRADPASMRPSELKSAAELLSGMRR
                     DVKKFDSADFADALANGDICLGVGYSGDSFRARDRAREANNGIEINYTIPKEGTLMSL
                     DNLAIPKDAPHAEEAYAFIDFLLRPAIAARNTNFTHLANGVLASKSSIGKDIVENKSI
                     YPDAAMLQRLFAVANHDPSARKTIAREWARVKTGK"
     gene            complement(2129..2275)
                     /locus_tag="VNY06_08180"
     CDS             complement(2129..2275)
                     /locus_tag="VNY06_08180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX14803.1"
                     /translation="MLPQIKIVRGLDGGVGVSDRDMHGPVFLKAGILRKFNKLPAGTS
                     SSIS"
     gene            2436..2807
                     /locus_tag="VNY06_08185"
     CDS             2436..2807
                     /locus_tag="VNY06_08185"
                     /inference="COORDINATES: protein motif:HMM:NF013540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HWX14804.1"
                     /translation="MAREEIQVNPDLIAWARKRAGLTLEEASKKFNRIAAWEAGDASP
                     TYPQLERLADKLKLPVAVFFFPELPSLPPIRESFRTLPDAEFDMIPRQVGFLLRKGNA
                     LQLNLAELSQDRNPAPASDYP"
BASE COUNT          605 a          829 c          843 g          588 t
ORIGIN      
        1 gagatagccc gagacgaagc gcgcggctaa gccgagatgg cggaggatct ggacgagcgc
       61 ccaggccgaa tcgcggcagg accccgaccc aagcgaaagg gtttcctccg gcgtctggac
      121 gcctggctcc atccggatca aatagcggat cgccttccgc aacgccgtat tgagatcgac
      181 gagaaaatca acgacgcgga tggggtcctt gggcaagccc gcgatatagg ccgcaaggcg
      241 cgggcccagc gtttcggcat cgaggtaagg cgcgagctgc gccttcaact cagggtcata
      301 agcgaaggga aattgctcgg catagggttc gataaaaaag tcgaaagggt tgatgaccgc
      361 aagttcggcg gtcagatcga cctcgatctt gaactccgtc accttctccg gaaagacgaa
      421 ccgcgcttgc caattgccat gcgggtcctg ctgccagttc acgaaatgct ccgccggcgt
      481 caccttcagc gaataggcgg ggattttggt ccggcagtgc ggcgccgggc gcagcctgat
      541 ggtctgcggc tcaaggacga ccggccggtc gtatttatag tgggtgatat gatggatggc
      601 ggcggaaatg gtcacgttcg atggtccgag tgccggaatc tctcggccag tattaatggt
      661 gcgccgcaaa agtgcaagcc gccggtgagc ggccgcgctc tagggccgcg ggcgcgggcc
      721 actttccaag ggttgttggt ggtttttaga tagagaccgg taggccagta gatcatggac
      781 atcggcgaaa tgcagagccc agagccaact accgtattgc cgctgtgttc gccggtgggc
      841 aggtccaggg aaaacgttgc ctatcggata gtgccggcgg gacgaaaagc tgggactatt
      901 aacatccgct ggctcttcaa gcatccgatc gattatttgg cccgccgcga acgcggaaac
      961 cggcttcgcg gcattatttg ccggttttta cccgcgccca ttcgcgggca attgtttttc
     1021 gcgccgatgg atcatggttt gccaccgcaa agagccgttg cagcatcgcg gcgtctgggt
     1081 aaatcgactt gttctcgacg atgtccttgc cgatcgagga tttggacgcg aggacgccgt
     1141 tggcgagatg agtgaaattc gtattgcggg cggcgatcgc gggccgcaag aggaagtcga
     1201 tgaaggcata ggcttcctcg gcatgcggcg catccttggg aattgcgaga ttatcgagcg
     1261 acatcaaagt gccttccttg ggaatagtgt aattgatttc aatcccatta tttgcctctc
     1321 gcgcgcggtc gcgcgcgcga aaactgtctc cggaatagcc aacgccaagg caaatgtcgc
     1381 cattggcgag cgcatccgca aaatcggcgg agtcgaattt tttcacgtcg cgccgcattc
     1441 cgcttaacag ttccgcggca ctcttaagtt cgctcggacg catcgacgcc ggatcggccc
     1501 taagatagtt aagcgcgatt gcaaacagat cttcgccgct gtccaggacg gtaacgccgc
     1561 agccggcgaa tttcttcaaa tttcttggct tgaagaggat gtcccaagaa tttagcgaag
     1621 catcggctgc cgcgtccccc aaaagctcct tggccttttc tacattgtag gcaatgcccg
     1681 ccgtaaacca catatagttg accgcatatt ggttgcccgg atcatagact gcaaggcgcg
     1741 ccatcacctc cggccaaagg ttcttgctgt ttggaagttt ggttttgtcg agtttttggt
     1801 agacccccgc cgcgatctgc ctttgcagga ccgggcccgg gacgatcaca acatcgaagc
     1861 cggtcttgcc cgccaggaac cgcgcctcga acgcctcgtt tgaatcgtac ccgtcatagg
     1921 tgaccttgat ccccgtctcc ttggtaaagt cttcgatgac cttgggatcg atatagtcgc
     1981 cccacccata gatgctgacg gccttctctt ggccgaacgc gggaagaagc ggcccgcaca
     2041 agcaaagtcc aaataaccaa agcgctgcga acgaattctt catgcgaagc ttgcaaaccg
     2101 ggggccgtag cgaattgatg ccgctcgctc aagagataga cgaacttgtg cccgccggca
     2161 atttgttgaa ctttcgcaaa atccctgcct taaggaagac cggaccatgc atgtcacgat
     2221 cgctgacgcc gacaccgcca tcgagaccgc gcacaatctt gatttgcggg agcatccctg
     2281 tcgtacttga cgcgcgtgga gcgttgagac gctgcagcga ttcactactg cgaggcgatc
     2341 cggcggggcg acccgggtga actgctccga gattgcgcgc ctttgcgata taagccacaa
     2401 cacaatttcg tggctcccgc catccagagc gccccatggc tagagaagaa atccaggtca
     2461 acccggacct tatcgcgtgg gcacgcaaac gcgccggact cacgctggaa gaggcgagca
     2521 aaaaatttaa ccgcattgcc gcctgggagg ccggcgacgc gtcgccgact tacccacagc
     2581 tcgaacggct tgcggacaag ctgaagctgc cggtcgcggt attctttttc cccgagctgc
     2641 cgagtcttcc gccgatccgg gagtcgttcc gcaccttgcc cgatgcagaa tttgacatga
     2701 tcccgcgcca ggtcggtttt ctgctgcgca agggcaacgc cctgcagctc aacctcgcag
     2761 agcttagcca ggaccgcaac ccggcacccg cgtctgatta cccgtgacgg cattgtcaca
     2821 ctgaattctg gtccttgata gccgccgtcg gcgaagagct ttttc
//
LOCUS       DATMPU010000357         2857 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_2757690,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000357 DATMPU010000000
VERSION     DATMPU010000357.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 2857)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2857)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2857
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2757690"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..225)
                     /locus_tag="VNY06_08190"
     CDS             complement(<1..225)
                     /locus_tag="VNY06_08190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX14805.1"
                     /translation="MLGVFLLAAAVLYSGVFAVLYSHAAYSELEFAEPGSTTPLNIYV
                     TMIDMDPVREELTVLLDFATEIGPHGKHFPG"
     gene            complement(339..776)
                     /locus_tag="VNY06_08195"
     CDS             complement(339..776)
                     /locus_tag="VNY06_08195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012335544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2497 domain-containing protein"
                     /protein_id="HWX14806.1"
                     /translation="MNTANPHSKDGQMAEAGPNEPSMAEILASIRRIIADDQALSGSL
                     GEVRKEAPRTGPDGEDMEPGAAREAARGGNQRSLLSGGAENSVNAAFQTLLASRFARH
                     GDVIIDLTREMLRPMLKAWLDANLPAIVERLISAEIERVSRSE"
     gene            complement(1372..2103)
                     /locus_tag="VNY06_08200"
     CDS             complement(1372..2103)
                     /locus_tag="VNY06_08200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018266092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HWX14807.1"
                     /translation="MQDTVFKGAGVAEPLSQSAARRQTMVDCQIRTFGVTDQQVLARM
                     LEVPREQFLPADQAQLAYSDLALQLKPTASGGEERRLLPPLVLARLMQGAEVGPADSV
                     LDVAPGTGYSSALLAGLAARVVALESEQSFYEALQSNLDRLGLANAQAQLGPLAVGAP
                     DEGPFDVIFINGKVETNLEALFAQLKDGGRLLALESVAGDLSGCAGKAVRYEKAGNAI
                     GYRVLFDAAAPVLRDFRKSEQFTFS"
     gene            2415..>2857
                     /locus_tag="VNY06_08205"
     CDS             2415..>2857
                     /locus_tag="VNY06_08205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020176114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4337 family protein"
                     /protein_id="HWX14808.1"
                     /translation="MSETPTEHFEHAEHAEHAALLGDPFLTQVSITIAILAVIAATIG
                     SLETLETAASMGDKNAAVLLQNRATDNWNFYQAKSIKKHLYTVGAANGGPMAEGFKKQ
                     VEGYGREEKELFAKGQALEHQTEEKLRDSEHHEHRHHILTISVTFL"
BASE COUNT          652 a          857 c          806 g          542 t
ORIGIN      
        1 gccggggaaa tgctttccat gcggcccaat ttcggtggcg aagtccagaa ggaccgtcag
       61 ttcctcgcgg actggatcca tgtcgatcat cgtgacatag atgttgagag gcgttgtcga
      121 tcccggctcg gcaaactcca gttcagagta ggcggcatgg ctgtagagca ctgcaaatac
      181 gccagaatat agaaccgccg ccgccagcaa aaaaacaccg agcatcgtcg cgcgccaacg
      241 aacagcggag ccgcgcgacg ttgtggtgag gggacgcgga cgaaaaacgt taatatttta
      301 attttggctg tttaaactgg cgcccctctt tggatcgttt actcgctgcg cgagacccgc
      361 tcaatctcgg cgctgatgag gcgctctacg atggccggca ggttggcgtc gagccaagcc
      421 ttgagcatcg ggcgcagcat ctcccgggtg agatcaatga tgacatcgcc atgcctggca
      481 aagcggctcg ccagcaaagt ctggaaggcc gcgttaaccg aattctccgc cccgccagag
      541 aggagcgagc gctggttgcc gccgcgcgcg gcttccctcg cagcgcctgg ctccatatct
      601 tcgccatcgg ggcccgtccg aggagcctcc ttgcgcacct cgccgaggct ccctgaaagc
      661 gcttggtcgt cggcaatgat tcgcctgatc gaagcaagaa tttctgccat cgacggttca
      721 ttcgggcctg cctcggccat ttgaccgtct ttcgagtggg gattggctgt attcatgatc
      781 tcaatatacc ttcaaacgag tgaaacgata ccttgaatac gcgatcgcgc aacggatcat
      841 ggggcggcgc cccttccagg cccgcccaag cgcaacgcgc aaagggcggg gccccaaccg
      901 cgatccatct gaacagagcg cgcgacgcca aactgccgtt cgctcgacac taagtaggct
      961 tccgtccctt ctatggaaca catcgttccg tcaacttttc caagagaaca cgattcgcgt
     1021 caacttggaa ctcttgtggc cctggtcttg tttggcctgt gcgttagccc gccacgagcg
     1081 ccccttagcc aaagggacag tgcgcggtcg gaatgaccgg ctgagagccc tcgggctctc
     1141 gcaaccgtac accaagttaa ccggattagc cttgacccaa actggcctgt gtcgtcgaga
     1201 acctcagtcg acccgcgcta cctaaggctc atccacagct tagcggccgc agaccccaag
     1261 ctcgcggcgg gaaaggcggc gagttttttg ggcggatgga cgcccaaagg tctgaaggag
     1321 ccgacgccca gggccgcggt taattacgca atgcaaaagt acgcggcact ctcaactgaa
     1381 ggtaaactgc tcgctctttc ggaaatcgcg gaggacgggg gccgctgcgt cgaacaacac
     1441 ccggtatcca atcgcatttc cggccttctc ataacgcacg gcttttcccg cgcaccctga
     1501 cagatctcca gctacgctct caagggcaag caaacggccg ccgtccttga gttgggcgaa
     1561 aagagcctcg agattggtct cgacctttcc attgatgaaa atgacatcga acggcccctc
     1621 atccggcgcg ccgacggcaa gcgggcccaa ttgggcctgt gcatttgcaa gccccagcct
     1681 gtcgaggttc gactgcaacg cttcgtaaaa tgattgctcg gattcaagcg cgacaacgcg
     1741 cgccgcaagg ccagcgagaa gcgccgagga atagcccgtg cccggcgcga catcgagcac
     1801 gctgtcggcc ggccccactt ccgcaccctg catgaggcgg gcgagaacca ggggaggcag
     1861 gagcctgcgc tcctctccgc cagaagcggt cggcttcaat tgcagcgcaa gatcggaata
     1921 agcgagctga gcctggtccg cggggagaaa ttgctcacgg ggaacttcga gcatccgcgc
     1981 caggacctgc tgatccgtta cgccgaacgt ccggatctgg caatcgacca tggtctggcg
     2041 acgggccgcc gattgagaca aaggctccgc aacaccggca cccttaaaaa cggtgtcctg
     2101 catgatttat tgccaatcct tatgtccggc cgactcctgc tcttaagggg agagcttata
     2161 gatatccccg gccgatctgt ccaaataaag tgcctaaggc agtcccgtca ggacacgcga
     2221 caagacaaag aattaaagca agatgatacg agtcgacgta cggccatctt gctctggcgt
     2281 ccgcgcaaaa cgccgagcat gcgtgcagcg tctgggcgag gacggcaaat cgttttggcc
     2341 tttctttggt ctatcattgc ggtcataaag acgcccggca aaaagcccac attttctgcg
     2401 cgaggaattc atcgatgtcc gaaactccaa ccgagcattt cgagcacgcg gaacacgctg
     2461 aacatgcggc acttttgggc gacccgtttc tcacccaagt gtccatcaca attgcgatcc
     2521 tagccgtcat agctgcaacc attggcagcc ttgagacgct agagacggcg gcgtccatgg
     2581 gcgacaaaaa tgccgcggtg ctgttgcaga accgtgcgac cgataattgg aacttttatc
     2641 aggcaaagag catcaagaaa cacctttaca ccgttggtgc cgcaaacggc ggcccgatgg
     2701 cggaaggttt caaaaaacag gttgaaggct atggtaggga ggaaaaagaa cttttcgcca
     2761 aaggccaggc gctggaacat caaaccgagg aaaagctaag ggacagtgag caccacgaac
     2821 atcgccatca tattctaacc atttcggtca cgttcct
//
LOCUS       DATMPU010000358         7542 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_1823141,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000358 DATMPU010000000
VERSION     DATMPU010000358.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 7542)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7542)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7542
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1823141"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2632
                     /gene="ileS"
                     /locus_tag="VNY06_08210"
     CDS             <1..2632
                     /gene="ileS"
                     /locus_tag="VNY06_08210"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020175182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HWX14809.1"
                     /translation="TVPINEFRRECRAFAEHWVGVQKEEFKRLGVLGDWEHPYLTMSF
                     AAEAQIAREIMKFAANGLLYRGSKPVMWSVVENTALAEAEVEYEDHVSDAIYVAFPII
                     EAWVQDQTAKRPVHLSAPDQLKKFGLDRTSIVIWTTTPWTLPANRAVSFSLKIKYGRY
                     RVTASSPGNWAKEGQIYVMADSLAPDVLKAARVDSFERVADFFPNPEGLFCAHPLAET
                     IAGYDFKVPLFHGEHVTEDTGTGFVHTAPSHGRDDFDIWMANKAALETRGIDTRIPYT
                     VDADGFFTKEAPGFEGRRVIDGKGNKGDANEAVIKALIEAGNLIARGKLKHQYPHSWR
                     SKKPVIFRNTPQWFIALDKPLAGKKTLRQIALSEIAATKWYPSAGENRITGMIANRPD
                     WVVSRQRAWGVPIAIFVHKETNEILVDDKVNLRIAEAFEQEGADAWFADGASVRFLKP
                     EHDPGHYEKINDVLDVWFDSGSTHAFTLDDPKRFPQFAGIHRLRDGGADEIMYLEGSD
                     QHRGWFHSSLLESCGTRGRAPYDAVLTHGFVLDENGQKMSKSLGNVVAPQTVIKDAGA
                     DILRLWVAASDYSDDLRIGPEILKTFVETYRKLRNTIRWMLGSLSHYDPASTVGFEAM
                     GELERLMLHRLAELNDEIRAAYAQFDFKRAVARLAAFLNTDLSAFYFDIRKDTLYCEP
                     PSSEKRRAALEAIEQIFRSVSLWLAPVLAFTADEAWLSRYPNALSVHLETFPEIPSSW
                     RDDALFQKWEKIRRVRSVVTGALEIERAQKRIGSSLEAAPRIFVVDPSLRSVLETVDF
                     AEICITSEIAIEAGDGPPYAFRLDDIAGVAVVPLRAEGRKCVRSWKISPLVGADPEFP
                     DVSPRDAEALRELRALGQWP"
     gene            2629..3129
                     /gene="lspA"
                     /locus_tag="VNY06_08215"
     CDS             2629..3129
                     /gene="lspA"
                     /locus_tag="VNY06_08215"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020175183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HWX14810.1"
                     /translation="MSPRGIGLWTMLATLFADQANKLWLIFVYGIEVRQPLRVTSFFD
                     IVFAKNPGISYSLLRAQTEAERLGLLLLIFAATAFLGGWLWKARTKMAGLGLGLIVGG
                     AIGNFYDRLAYGFVADFYHFHLGTFSWYVFNIADVGIVFGVGILIYVSLFPAKKPCEA
                     ERTLGN"
     gene            3251..3922
                     /locus_tag="VNY06_08220"
     CDS             3251..3922
                     /locus_tag="VNY06_08220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX14811.1"
                     /translation="MNFDVNIRRRFSFAVATATFLSAGGFAGIPQARAEDASSFKSFL
                     GYFGVKSNNDEEAIDYHPRAPLVVPPRLDLRQPKEAARDPSWPKDPDIAAQRRAALDS
                     HTPVAQTTANSRTGTSQTQTQQGSSALPAEGPPDECEASSGTALCLSTPWKALKSVAN
                     VFHSETIQPGPEPPRKYLIEPPPGYREPTSVAKATGEATKDKPDAAEPASPVRSQRPK
                     TSIEN"
     gene            4125..5510
                     /locus_tag="VNY06_08225"
     CDS             4125..5510
                     /locus_tag="VNY06_08225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014890804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HWX14812.1"
                     /translation="MAFGESNPSRESVSLNPSARAEEKLVGPAITQAKLSNGLDIVII
                     EDHRAPVVTHMVWYKNGSADDPVGKSGIAHFLEHLMFKGTKNNPQGKFSELIASLGGQ
                     ENAFTSNDYTAYFQRVAKEHLGVCMDYESDRMKNLVLTDEVIGPERDVVLEERRMRTE
                     SDPSEQLGEALQAALFTQHPYGRPIIGFAHEIETLDRGDAFAYYDRFYSPDNAILVVA
                     GDVAAKDVIGLAEKFYGSIQAHGERLLRFRPREPVPRAHRLVTLADEKVEQPLHQSVF
                     LVPSYRSAAPGDAEALEVLAYLLGGGHTSLLFKSLVLDRKLAVSTGAHYQGTAVDDTR
                     FYVYAVPLPGVGLETLDAAIDEVIAGVAARGVDEADLRRAKTRLVAETIYAQDNHAAL
                     ARWYGASLAAGLALEDVAEWPQRIESVTANAVRKAALWLDKRRSVTGFLLPAEPGVPA
                     KSTDAAGPPAS"
     gene            5558..6838
                     /locus_tag="VNY06_08230"
     CDS             5558..6838
                     /locus_tag="VNY06_08230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018267170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HWX14813.1"
                     /translation="MTALAAVHSPSRASMVQKPVSKGGIDAWLVEDYTVPLIAVEFGF
                     KGGASQDPIGKAGAATLLAGLLDEGAGPYDSEGFHRALDEDAIEMSFSADRDILTGRM
                     QTLSRNADRAFALLRLATTQARLDPEPFERVAGQIAAGLKREANDPDFVAGRRFRALA
                     YPNHPYGLPVRGDLSTLSSLTSADLHAMRASVFARDNLKIAAVGAIDATTLAGYLDDV
                     FGQLPGKADLTPIGAASFCGIGTRDIAAIDLPQSTIRFGREGLARKDPDFIAGMVVNH
                     ILGGGIFSARLFREVREKRGLAYSVYSQLVTHDRAAMFSGGTSTKNERAAESLAVIEK
                     EIVSLSERGSTEDELDKGKKYLIGSYPLRFDTSTKIAAQLASLQLEDFGVEYLDERNR
                     LIAAVTMEDARRAAKRLFGDGKLLVVIAGRPERM"
     gene            complement(6912..7542)
                     /locus_tag="VNY06_08235"
                     /pseudo
     CDS             complement(6912..7542)
                     /locus_tag="VNY06_08235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010687405.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
BASE COUNT         1572 a         2127 c         2202 g         1641 t
ORIGIN      
        1 cacggtgccg ataaatgagt tccgccgcga gtgccgcgct tttgccgaac attgggtcgg
       61 ggtgcaaaaa gaagaattca agcggctcgg cgtgctcggg gattgggaac atccctatct
      121 cacgatgagt tttgccgccg aagcccagat tgcccgcgag atcatgaaat tcgccgccaa
      181 cggactgctc tatcgcggct cgaagccggt gatgtggagc gtggttgaaa ataccgcgct
      241 tgccgaggcc gaggtggaat atgaagacca tgtgagcgac gcgatctatg tggcgtttcc
      301 gattatcgag gcgtgggtac aggaccaaac cgcgaagcgc cctgtgcatt tatcggctcc
      361 tgaccaactg aagaaatttg gtcttgatcg cacttccata gtgatctgga caacgacgcc
      421 gtggacgttg cctgctaata gggcggtctc gttctctttg aaaatcaagt atggacgcta
      481 tcgtgtcact gcctcttcgc cgggaaactg ggcgaaagaa ggccaaatct atgtcatggc
      541 ggacagcctt gcccccgacg ttttgaaggc ggcgcgcgtc gactctttcg agcgggtggc
      601 agattttttt ccgaatcctg aaggtctttt ttgcgctcat cctctagcag agacgattgc
      661 cgggtacgac ttcaaggtgc cgttgttcca cggcgagcac gtcaccgagg acacgggcac
      721 cggtttcgtc catacagctc cttcccacgg tcgcgacgac ttcgatattt ggatggcaaa
      781 caaagctgcg ctcgaaacgc gcggcatcga cacgcgcatc ccctacaccg tcgacgccga
      841 tgggttcttt acgaaggaag cgcccgggtt cgaaggccgc cgcgtcatcg acggcaaagg
      901 caacaagggc gacgccaatg aggctgtcat caaggctctg atcgaagctg gcaatctcat
      961 cgcgcgcggg aaactaaagc accaatatcc gcattcctgg cgctcgaaaa agccggtcat
     1021 ttttcgcaac acgccgcaat ggtttattgc gcttgataag ccgcttgcgg gcaaaaagac
     1081 gctgcgccag atcgcactca gtgaaatcgc cgcgacaaaa tggtatcctt ccgctggcga
     1141 gaaccggatc acgggaatga tcgcaaaccg cccggattgg gtggtctcgc gccagcgcgc
     1201 ctggggcgtg ccgatcgcga ttttcgtcca taaggaaaca aatgaaatcc ttgtcgatga
     1261 caaggtcaat ttgcgcattg ccgaggcatt tgaacaggag ggggccgacg cctggttcgc
     1321 ggacggcgcg tcggtgcgat tcctgaaacc tgaacacgac ccgggacact acgagaagat
     1381 caatgacgtt ctcgatgtct ggttcgattc gggatcgacc cacgctttca cgctcgatga
     1441 cccaaaacgc ttccctcaat tcgcgggcat ccaccggttg cgcgatggcg gagcggacga
     1501 gatcatgtat ctcgaaggct ccgaccagca tcgcggctgg ttccattcct cgctgcttga
     1561 atcttgcggc acgcgcggcc gcgcgcctta tgacgcggtg ctcacgcatg gcttcgttct
     1621 cgatgagaat ggccagaaga tgtcgaaatc gctcggcaat gtcgtcgcac ctcaaaccgt
     1681 catcaaggat gccggcgccg atattttgcg gctctgggtt gcggcatcgg actattccga
     1741 tgatctgcgc atcgggccgg aaatcttaaa aactttcgtc gagacctatc ggaaactgcg
     1801 caacacgatc cgctggatgc tcggctcgct ttcccattac gaccctgcga gcacggttgg
     1861 ttttgaagca atgggcgagc ttgaaagact gatgctgcat cgtcttgcgg aacttaatga
     1921 cgagatccgc gctgcctatg cgcagttcga ctttaagcgc gcggtcgcgc ggcttgcggc
     1981 ctttctcaac accgatcttt ccgcctttta cttcgacatt cgcaaggaca cgctttattg
     2041 cgaaccgcca tcgagtgaaa agcggcgcgc cgctctcgaa gcgatcgaac agattttccg
     2101 ctccgtcagt ttgtggcttg ccccggttct cgcctttacg gcggatgagg cgtggctctc
     2161 gcgctaccca aatgcgctct cggtgcatct cgaaactttt ccagagattc cgtcaagctg
     2221 gcgcgatgat gcgttgttcc aaaaatggga aaaaatccgc cgcgtccggt ccgttgttac
     2281 cggcgctctt gagatcgagc gggcgcaaaa acggatcggc tcctccctcg aggccgcgcc
     2341 acggattttt gtggtggacc cctcgttacg gagcgttctc gaaacggtcg attttgccga
     2401 gatctgcatc acttcggaga ttgccatcga agcgggggac gggccgcctt atgcattccg
     2461 gctcgatgac atcgctggcg tggcggttgt gccattgcga gccgaaggac ggaaatgcgt
     2521 tcggtcgtgg aaaatttcgc ccctcgtcgg cgccgatccc gaatttccgg acgttagccc
     2581 ccgcgatgca gaggctcttc gggagctgcg ggcgttggga caatggccgt gagcccgcgc
     2641 ggtatcggcc tatggacgat gcttgccacc ttattcgcag atcaggcgaa caagctttgg
     2701 cttatctttg tatatggtat cgaggtgcgc cagccgctca gggtcacctc tttcttcgat
     2761 atcgtctttg ccaaaaatcc tggcatttcc tattcgctgt tgcgcgcgca gacggaagct
     2821 gagcgccttg gcttactcct gcttattttt gcggccacgg cctttttggg aggttggctt
     2881 tggaaggcca gaacgaaaat ggcgggtctc ggcctcggcc taattgttgg cggagccatt
     2941 ggtaatttct atgaccggct ggcctatgga tttgtcgccg atttctatca ttttcactta
     3001 ggcacgttct cgtggtacgt tttcaatatt gccgatgttg ggatagtatt tggcgtaggg
     3061 attctcattt acgtttcgct gtttccggca aagaagcctt gcgaggcaga gcgcaccctc
     3121 ggaaattgag aaagcctctc tctgcctcta aaaagccagg aatgaagaca aaataacccg
     3181 gaaggaaatt acgcggtgag cttcgccata cagcggagcg cgctctttaa gaatttaggg
     3241 gaatgttgac atgaacttcg acgtcaatat acggcgtagg tttagctttg cggtggcgac
     3301 ggccactttc ctttctgccg gaggctttgc cggtataccg caggcgcgcg cggaggacgc
     3361 cagttcgttc aaatccttcc ttggctattt cggcgttaag tccaacaacg acgaagaagc
     3421 aattgattac cacccaagag cgcccctcgt cgtgcccccg cgccttgacc tacgtcagcc
     3481 gaaagaagcg gcgcgcgatc cttcctggcc gaaggatccg gatattgccg cgcagcgccg
     3541 cgcggccttg gattcgcata cccccgttgc gcagacgacg gcaaattccc ggaccggaac
     3601 gtcgcaaaca caaacgcaac agggaagcag cgctctgccg gcggagggac cgccggacga
     3661 gtgcgaggcg agctccggga cagcgctgtg cctctcgaca ccctggaaag ccctgaaatc
     3721 ggtggcgaac gtctttcatt ccgagactat ccagccggga ccagagccgc ctcgaaaata
     3781 tctgatcgag ccgccaccag gctatcggga gccgacaagc gtcgccaagg cgaccggcga
     3841 ggcgacgaaa gataagcccg atgccgccga acccgcgtcc cctgtccgct ctcagcggcc
     3901 gaagacctcg atcgaaaatt gacccaaagc ccggagcctc gtccagacgg ccagcatggc
     3961 cctcttgaca aagaccggag ggcgctgggc aacaaaaccc tgacactttc tgagattcca
     4021 ttctatattc gcgcagactt tgactgcgta atggatagcg agggatcgcg gcgggttttt
     4081 ctcccgctga cgcggttttt cataaatcgg aaagtgatgg cgaaatggct tttggtgaat
     4141 cgaacccatc tcgggagagc gtttctctaa acccctccgc gcgagcggag gaaaaattgg
     4201 tcggtccggc catcactcaa gctaaactat ccaacgggct cgatatcgtt atcatcgagg
     4261 atcaccgcgc gcccgtcgtc actcatatgg tctggtataa aaatggctcg gccgatgatc
     4321 cggtgggcaa gtcaggcatt gcccatttcc tcgaacattt gatgttcaaa ggcaccaaga
     4381 acaatccgca aggcaaattc tccgagctta tcgccagcct cggtggccag gagaatgcat
     4441 ttacctcgaa cgattacacc gcctattttc agcgcgtcgc caaggagcat ttgggcgtct
     4501 gcatggacta tgagtccgac cgcatgaaga atcttgtcct gaccgatgag gtaattgggc
     4561 ccgagcgcga tgtggttcta gaggaacggc ggatgcggac cgagtccgat ccttccgagc
     4621 agcttggcga ggcacttcag gcggcgctat ttacccagca tccctatggc aggccgatca
     4681 tcggctttgc acatgagatc gagactctcg accggggaga tgcctttgcc tattacgatc
     4741 gcttttactc gccggataat gccattctcg ttgtcgccgg ggatgtcgcg gcaaaagacg
     4801 tgatcggact tgcggagaag ttttacggtt ccatacaagc gcatggcgag cggcttttgc
     4861 ggtttcgtcc gcgcgagcca gtgccgcgcg cgcatcggct cgtgaccctt gccgacgaaa
     4921 aggtcgagca acccttgcac caaagcgttt ttctggtgcc ctcctatcgc tcagccgcgc
     4981 ctggcgacgc cgaggccctt gaggtccttg cctatttgct cggcggcggc catacgagcc
     5041 tattgttcaa gtccttggtc ttggaccgca agctcgcggt ttcgacgggg gcgcattacc
     5101 agggcacggc ggtcgatgac acgcggtttt atgtttatgc cgtgccgctg cccggtgtcg
     5161 gcctcgaaac gctcgatgct gcaatcgacg aggtgattgc tggggttgcc gcgcggggtg
     5221 tcgatgaggc cgatttgcgg cgcgccaaaa cccgcctcgt ggcggagacg atttatgccc
     5281 aggacaatca tgcggcgctc gcccgctggt atggcgcgtc gttggcagct ggcctggctc
     5341 tcgaggacgt cgcagaatgg ccgcagcgta tcgagtcggt caccgcgaat gccgtgagaa
     5401 aggcggctct ctggctcgac aagcggcgca gtgtgacggg ctttctcctg ccggctgaac
     5461 ccggcgtccc agcaaaaagc acagatgcgg ccggcccgcc cgcatcttag atgcatttag
     5521 gccagccttc cgcaacacct agcccaggga agaacacatg accgcgcttg ccgccgttca
     5581 ctccccctca cgcgcatcaa tggtacaaaa gcctgtctca aaagggggca tcgacgcttg
     5641 gctggtcgag gactatactg ttcctctcat agccgttgaa ttcggcttca agggcggagc
     5701 ctcgcaagac ccgattggca aagcaggcgc cgccacgctt ctcgctggtc ttcttgacga
     5761 aggcgcaggg ccctatgatt cggaaggctt tcatcgtgcc cttgatgaag atgcaatcga
     5821 gatgtctttt tccgcggacc gcgacattct cactgggcgc atgcaaaccc tgtcgcgaaa
     5881 tgcggaccgt gctttcgcgc tgttgaggct cgcgacaacg caagcgcgcc tcgatccgga
     5941 gccttttgaa cgggttgcag gccagatcgc cgccggcttg aagcgcgaag caaatgaccc
     6001 cgacttcgtt gcggggagaa ggtttcgcgc tttggcctat cctaaccatc cttatggcct
     6061 tcctgtgcgc ggtgaccttt cgactttgtc gagcctcacg tccgccgatt tgcatgctat
     6121 gcgcgcttcc gtgtttgcgc gggacaattt gaaaattgcc gcagtcggcg ccattgatgc
     6181 gacaacgctt gccggctatc ttgacgatgt ttttggtcaa ttgccgggaa aggcggatct
     6241 cactccaatt ggagccgcct ctttttgtgg tatcggaacc cgcgatatcg ctgcgatcga
     6301 cttgccgcaa tcgaccattc ggtttggccg agaagggctt gcccggaaag atcccgattt
     6361 catcgccggg atggtggtca atcatatctt gggcggaggg atcttttcgg cgcgcctgtt
     6421 tcgggaggtg cgggagaagc gcgggctcgc ctattcggtc tattcccaac tcgtgaccca
     6481 tgatcgcgcc gccatgtttt ctggcggcac gtcgaccaag aatgagcgcg cggccgaatc
     6541 gctagctgtc atcgaaaagg agatcgtgag cctttcggag cgaggatcga cggaggacga
     6601 actcgacaag ggcaagaaat atctgatcgg ctcctatccg ctgcggttcg atacctcaac
     6661 gaagatcgct gcgcagctcg cttctctgca attggaagat ttcggggtcg aatatctcga
     6721 cgagcgcaac cggctgattg ccgctgtgac catggaagat gcccgaaggg cggcaaaacg
     6781 cctctttggg gacggcaaac tcttggttgt gattgcaggc cgccccgaaa gaatgtgagg
     6841 gcaggcgagc tatggggtat taatagctgc caaattgggg acagcaggcc atctgcgcga
     6901 ccgtgccctg tctaataaga cactgtccga ctgacctgaa tgacagccgg cgtcatgatg
     6961 actaggaaaa gcacggggag aaagaacaat atcattggca cggtgagttt gggcggcaat
     7021 gcggccgctt ttttttcggc gagcatcata cgccgatttc ggctttcctg ggcaacgaca
     7081 tttagggcgg cgccaagcgg tgtgccgtag cgctccactt gtataagaac gatgacgatc
     7141 tgcttaatcg agtcaagacc tgttcttatc ccaagatttt tcaaaagccg aacgccggtg
     7201 gggaagaaag gagagttccg ccgtggtcaa cgtcagctcc tcggcaagcg gtacggactg
     7261 gaggccgatt tcctgcgaga ccttgcgaaa ggcgtgttcg atcgacatgc cggactcgac
     7321 gcagataagc aacaaatcca gcgcgtcggg aaaggcgcgg cccattgaga attgacgctt
     7381 gctgatctta ttcgagaggt agagttccgg tgccttcagg ccgatatagg tggcgaaaag
     7441 gattacgcca gcttccatcg tgacactcag ttccgtagga ggcatgacaa aaatgtagaa
     7501 ggcgcagacc gcgaaactta aaactggcat aaccagacgg aa
//
LOCUS       DATMPU010000359         4157 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_2759343,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000359 DATMPU010000000
VERSION     DATMPU010000359.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 4157)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4157)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4157
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2759343"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3124
                     /locus_tag="VNY06_08240"
     CDS             <1..3124
                     /locus_tag="VNY06_08240"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HWX14814.1"
                     /translation="NSVALSTRLDPEDLRDVMAAYHRTCAQSVRRYNGFIARYMGDGI
                     LIYFGYPAANEDDAERAVRAGLELVDAVVKLNNSMNPFTDLNLQVRVGIATGLVVVGD
                     EIAENVLDKDAITGEAVNLAARLQTLAEPNTVVSSATTRQLAAERFAYRELGLREIKG
                     FDTPVPVYQVIGEREVSRFAARGAALTPFVGRDKEIEILLAGWARAVSGNGQVVIVSG
                     EAGIGKSRIAAEVCSRIRRSDPDILSPFFYQCSPYHTNAPLYPVIKELERVAEIRRSQ
                     PARENLKKLKTLLRIGERGDDRQLTLLADLLDLPPDERPPPLALSAAVKRSLTIEALI
                     GWVARCAHEKPLIIVFEDVQWIDPTSKLLLNRLVDWAQAVRALIMITLRIESSGAEKW
                     CEEAGLSTIHGQRRSFVTICEIGELGAIEATRLAAAAAEGRTISDRQLNAILTKSDGI
                     PLYLEELVRAVVDRSDFSPNHEEADQSDGVPNTLRDALMAQLDRLGRAKEVAQHASVI
                     GQEFSASLLAKVANKPIEDLLPELHSLINSKIVVQAGTTSETYRFKHALLRDISYRSL
                     LRKMRRQIHLSVAGELAHSRTEAVDATDDLIAQHYSRGTAYSEAIQFWQIGAKTAIAR
                     SAYEEALAMLQAARTDLRKLPSSEWRVVELDLVLAQAIALRSLRGYSAPEVEERLLEA
                     RELCSVCADNINRFNVEWGLFQCTIVKRDIEAARQLAAGLFEHARRHPNIPVVDAYLA
                     NGMVAVNAGEFETARRFLEKGVSLSRPETDQPHFFAHGQNPGLFCLSYLARTLCFLGY
                     LDRCRAVMNRCLSIASKRAHDPGHIYGYVNALMHAVRVYNLCGDLDAEERVAKETNEI
                     SRRNHYAYYEALSTCHLAWVAGARGFLSEGIERMICGLAALGQTGTSLALPGLYVLLA
                     QLYIRAGQWDEANNVLQMAVGVRGHAVWDADVERVRGDLLSLRADPDLSAAEATYRSS
                     LTIARRQRAGLLEFKAGLNLARLLRDLHRREEGFEILSVCLEKLHGEFGTADARNVRI
                     LMNDLAVTGR"
     gene            3305..3775
                     /locus_tag="VNY06_08245"
     CDS             3305..3775
                     /locus_tag="VNY06_08245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX14815.1"
                     /translation="MAIASRDYQGLEVVFDQYLFPYCISDANERSRAVTFLRRYWFDP
                     GSPDAFFPGFPLARIYAEGVLKALELSLNGRRVVPLNAWWILDTTEPKMLVLADVNDQ
                     GVTVGGRVTLLVATPRPSGGGRPRGAAILGQTAQAWVSQRQDGQVTTNSVRDLQ"
BASE COUNT          875 a         1115 c         1290 g          877 t
ORIGIN      
        1 taattctgtc gctttatcga cccggctcga tcccgaagac ctgcgggacg taatggccgc
       61 ctatcatcgg acctgcgcgc aatcggtcag gcgctacaat ggctttatcg cgaggtatat
      121 gggcgacggc atattgatct atttcggcta ccctgcggcg aatgaagacg atgccgagag
      181 ggctgtgcgt gccgggctcg aactcgtgga cgccgtcgtc aagctgaaca acagcatgaa
      241 tccctttacc gatctgaatc ttcaggtgcg cgtcggcatc gccacggggt tggtcgttgt
      301 cggcgatgaa atagcggaaa atgtcttaga caaggacgct atcaccgggg aggcggtcaa
      361 tcttgccgcc cggctgcaga cgctggccga gcccaacact gtcgtctcct cagccacgac
      421 gcggcagctg gcagctgaga ggttcgcgta tcgcgaactg ggcttgcgag agatcaaagg
      481 cttcgacacc ccggttccag tatatcaagt catcggcgag cgggaggtca gccggttcgc
      541 tgcgagaggc gcggccctca ccccgttcgt cggtcgagac aaagaaattg aaatccttct
      601 tgccggttgg gcccgcgcgg tatccggcaa tggtcaagtg gtgatcgttt cgggggaggc
      661 gggtatcggg aaatcgcgta ttgccgcaga ggtatgttct cgaattcggc gcagtgaccc
      721 cgatatactg tcgccgtttt tttatcaatg ctccccatat cacacgaacg caccccttta
      781 cccggtaatt aaggagttgg aacgggtggc ggagatccgc cgatctcagc cagctcgaga
      841 gaatctgaaa aaactgaaaa ccctcctcag aattggggag cgaggcgacg accggcagct
      901 cacactgctt gctgatctgc tggatctccc cccggacgaa cgcccgccgc ctctcgccct
      961 aagcgcagcc gtcaaaagaa gtctcacgat agaggcgcta atcggctggg tcgcgcgctg
     1021 cgcgcacgaa aagccgttga taatcgtgtt cgaggacgtg cagtggatcg atcctaccag
     1081 caagctactg ctcaaccggt tggtcgattg ggcacaagcg gtacgagctt taataatgat
     1141 aacgctgcgg atcgagagtt ccggcgcgga aaaatggtgc gaggaagctg ggctttccac
     1201 tatccacggc cagcgccgat cgttcgtgac catctgcgag attggcgaac tgggtgcgat
     1261 cgaagcgacg cgcttggcgg cggcagcagc cgagggcagg acgatttccg acagacaatt
     1321 gaacgctatc ctgacgaagt cagacggcat tccgttgtat ctcgaggaac tggtaagggc
     1381 tgttgttgat cgcagcgatt tctcgccaaa tcacgaagaa gccgatcaaa gcgacggcgt
     1441 accgaatacg ctgcgcgacg cattgatggc gcaactcgat cggcttgggc gcgcgaaaga
     1501 agtagcacaa catgcctcgg tcatcggcca agagttctcg gcaagcttgt tggccaaagt
     1561 cgcgaacaag cctatcgaag atttgctccc cgagctgcat agcctgatca attcaaagat
     1621 cgtagtgcag gctgggacga cctccgagac ctatcgcttc aagcatgcgc tgctgcgcga
     1681 tatctcttac cggtcgcttc tcagaaagat gcgccggcaa attcacctca gcgttgccgg
     1741 cgaactcgct cattcgagaa cggaggccgt cgatgccacc gacgacctga ttgcccaaca
     1801 ctattcccga ggtacagcct attccgaagc aatccaattt tggcaaatag gtgccaagac
     1861 cgccatcgcc agatcagcgt acgaagaagc gctcgccatg ttgcaggcgg cccgaaccga
     1921 cctgcgaaaa ttgccgtcct cggagtggcg cgttgttgag cttgatttgg tcttggctca
     1981 ggcgatagcg ttgcgctcgc tgcgcggcta ttccgcgcct gaagttgagg agcggttgct
     2041 tgaggcccgt gagctttgct ctgtttgcgc cgacaacatt aatcggttca atgtagaatg
     2101 ggggctgttt caatgtacga tcgtcaaacg cgatattgag gctgcacggc aactggctgc
     2161 tggtcttttc gaacatgcca ggcgccatcc gaatataccg gtggtcgatg cctatctcgc
     2221 taatggcatg gttgcggtca atgccgggga gttcgagaca gccaggcgct ttcttgaaaa
     2281 aggcgtcagc ctgagccgcc cggagaccga tcagccgcat ttctttgccc acgggcaaaa
     2341 tccggggctg ttctgcctgt cgtacttggc tcgtaccttg tgttttcttg gatatttgga
     2401 ccgttgccgg gctgtgatga accggtgtct ttcaatcgca tcaaaacgag cgcatgaccc
     2461 cggacacata tacggttacg tcaatgccct gatgcatgcg gtgcgggtat acaatctatg
     2521 cggcgatctg gatgctgaag aacgagtggc caaggagacc aacgaaattt ctcgacgtaa
     2581 tcactatgca tactacgagg cgttgagtac gtgccacctt gcttgggttg cgggcgcccg
     2641 ggggttcctg tcggagggga tagagagaat gatttgcggt ctcgccgcgc ttggacagac
     2701 gggcacatca ctggcgcttc cgggtctata cgtgctattg gcgcagttgt atatccgtgc
     2761 cggtcagtgg gacgaggcga ataatgtgct gcaaatggcg gtcggagtta ggggccatgc
     2821 ggtatgggat gccgatgtcg aacgagtgcg cggcgacctg ttgtcattac gcgccgatcc
     2881 cgatctgtcg gcggccgagg ctacataccg gtcgagctta accattgcac gacgccaacg
     2941 ggctggctta ctggagttca aggccgggct gaacttggcc cgactgctgc gcgacttgca
     3001 tcgtcgcgaa gaaggcttcg agatcctctc ggtatgtctc gaaaaactgc atggggaatt
     3061 tggcactgct gatgcgcgaa atgtgcgcat actcatgaac gatctcgccg tcacgggtcg
     3121 ctagaccccg agggcgtcgc atttgttgcg ggcgggaatg cccaggcgtt aggggaggaa
     3181 tgatttggct gatcctgtac aaccgggcgt accgtgcgca aaggggatcg tctgctattg
     3241 tttggacgag ttttctaagg ggaggggggc ccggacgcgg ctcaccagac tgagggatgc
     3301 aattatggca attgcctcca gagactatca ggggttggag gtggtgtttg accaatattt
     3361 attcccgtac tgtatcagcg acgccaacga gagaagcagg gcggttacct tcttgaggcg
     3421 atattggttc gatcctggct cgcccgacgc gttcttcccg ggtttcccgc tcgctcgaat
     3481 ctatgccgag ggggtgctaa aagcgcttga attatcgttg aatgggcggc gggtcgttcc
     3541 gctcaatgct tggtggatcc tcgatacgac agaaccgaag atgctggtct tggcggacgt
     3601 aaacgatcaa ggtgttactg tcggcggtcg agtgacgttg ctggttgcga cgccaagacc
     3661 gagcggcgga ggcagaccta ggggcgccgc gatcctcggt cagacagctc aggcgtgggt
     3721 atcacagcga caagatggcc aagtcacaac caattccgtc agagaccttc agtagaggga
     3781 gcggggaaat cgcacccggc tgccaacgtc ctaatccctg aggacgacga cttcgagctc
     3841 ccagcatccc gaggtaatct cgagttgcgc ttgtcgagcc gtgtgctcat tcaaccttac
     3901 aggcgaagca cgagaaacgg agacctgggt ggctatcggc tgtcggacct gcgaacaatc
     3961 cgcgccgaga gcatcggggc ggaaaggggt gccgccgagc cctgggcggc aggcttgttt
     4021 gacgacatgc tcgaagccat ggggcccgtt gagaaactgt ccggccggca tcgtgcaatg
     4081 gtggcacggt attgcgaaga cgtgtaccgg ctgatgtcgg agatggcgcg cgtcctcaag
     4141 ccgggtggga aagcgat
//
LOCUS       DATMPU010000360         3641 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_2162436,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000360 DATMPU010000000
VERSION     DATMPU010000360.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 3641)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3641)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3641
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2162436"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..151
                     /locus_tag="VNY06_08250"
     CDS             <1..151
                     /locus_tag="VNY06_08250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003509369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HWX14816.1"
                     /translation="EWVKRCPNPHDEDCEIEIRQIFESEDFGAALTPELRAQEERLRA
                     QTAAR"
     gene            200..649
                     /locus_tag="VNY06_08255"
     CDS             200..649
                     /locus_tag="VNY06_08255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013166964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HWX14817.1"
                     /translation="MQPYLSFDGRCEEAIEFYRKALGAEMQMLMRFKEAPDPYKCQPG
                     SEDKVMHMCFRIGDTALFASDGRCEGKPNFQGISLSLTAADDAEAERLFNALADGGQV
                     QMPLAKTFFSSRFGMVADRFGVPWMVLVKSLRAESLAVAGHTKRRDP"
     gene            653..1147
                     /locus_tag="VNY06_08260"
     CDS             653..1147
                     /locus_tag="VNY06_08260"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HWX14818.1"
                     /translation="MQLVKPKEDLVVIITRMFDAPRELVFQAFNDPKHIVRFWGPNGF
                     TSTVRELDPRPGGAFRIDMRAPDGATYLCEGVYREIIVPQRIVLSGDPDCGHPCGGGL
                     PPGALVAITFTEHGSKTKLTIQTRFESQMGLDAAVEMGFNGSWTQTLDRFAAELEKTL
                     IQRF"
     gene            complement(1404..2231)
                     /locus_tag="VNY06_08265"
     CDS             complement(1404..2231)
                     /locus_tag="VNY06_08265"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020177020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HWX14819.1"
                     /translation="MLLEPISHGDKLVAAFKEVVCGAGKLALGYYRPGAPTTAGIAHK
                     TGGSPVTEADCLVDRYLAQQLMPLLPEAGWLSEETEDSPARLSRDLILVIDPIDGTRG
                     FMTGQSGWAIAVALIEKGRPLLGIIHAPVLGETYIAVRGAGAKLNDKTIAVSKLAAMN
                     AEARVVAPGFLADRLRQAGLKFDLRPRIPSLALRIANVASGALDAGFASENARDWDIA
                     AADLILHEAGGRLATLDGSEILYNRQKTHHGVLTAAPERIHSEVNAAARLANYKLQG"
     gene            complement(2395..3420)
                     /gene="cobT"
                     /locus_tag="VNY06_08270"
     CDS             complement(2395..3420)
                     /gene="cobT"
                     /locus_tag="VNY06_08270"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018390051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008939 -
                     nicotinate-nucleotide-dimethylbenzimidazole
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HWX14820.1"
                     /translation="MIPSVKTLNSIRELLASLPQASEASRWAAQARQAELTKPPGSLG
                     RLEEIPAFLAAWQGKPVPSLDRPMVAVFAANHGVVEKNVSAYPASVTGAMVKNFSVGG
                     AAINQICKVNGLGLVVFDLALDIPSKDITEEPGLEEEETAASFAFGMGAVPGGVDLLC
                     LGEMGIGNTTIAAAIYHGLYGGAAADWVGYGTGIDDHAFERKTAAVKAAALRHNDHLA
                     DPLQVLRRLGGREFAAIAGAILAARLQRIPVVLDGYVVCAAAAILHAINKTALDHCLA
                     AHVSAEGAHKEVLRRLGKEPLFDLRMRLGEGSGAALAFAIIKAAVACHSGMATFAEAK
                     VAGRSNQ"
BASE COUNT          788 a         1090 c         1012 g          751 t
ORIGIN      
        1 cgaatgggtc aagcgctgcc caaatcccca tgatgaggat tgcgagattg aaatccgaca
       61 aatctttgag tctgaagatt ttggcgcggc gttgacaccc gaactccgcg ctcaggagga
      121 acgcctccgc gcacaaaccg cagcgcgctg aggccgagac ctccattaaa aaaatcttca
      181 tcaccacaga acaaggagag tgcagcctta cctttctttc gatggccgct gcgaggaagc
      241 aatcgaattc taccgcaagg cgctcggcgc cgaaatgcag atgctcatgc gcttcaagga
      301 ggctccggat ccatataagt gccagcctgg tagcgaagat aaggtgatgc acatgtgttt
      361 ccgtataggc gatactgccc tttttgcatc cgacggccgc tgtgaaggca aacccaattt
      421 ccagggcatt tcgctgtctc tcacggcagc tgatgatgcc gaagccgaac ggctgttcaa
      481 cgcgctggca gacggtgggc aggtgcaaat gcccttggcc aagacctttt tttcgtcgcg
      541 tttcggcatg gttgccgacc gctttggcgt gccttggatg gtcctcgtaa agtctttaag
      601 ggccgaatcc cttgctgtcg ccggtcacac gaaaagacga gacccatgac cattgcaact
      661 ggtcaaaccg aaggaagacc ttgtcgttat catcacgcgc atgttcgatg caccgcgtga
      721 actggtcttc caggcattta acgatcccaa gcacatagtc cggttctggg gcccgaacgg
      781 ctttacaagc accgtccggg aactggatcc tcggccagga ggcgcctttc gcattgacat
      841 gcgcgctccg gacggcgcga catatctgtg cgagggcgtc tatcgagaaa tcatcgtacc
      901 gcagcgtatc gttttaagcg gcgaccccga ctgtggccac ccttgcggcg gcgggctgcc
      961 gccaggcgcg cttgttgcga ttacctttac cgagcacggc agcaagacga agctcacgat
     1021 acaaacgcgc ttcgaatcgc aaatgggcct tgacgcggca gtggagatgg gtttcaacgg
     1081 gagttggacg caaaccttgg accgctttgc ggctgagctt gaaaaaacgt tgatccaaag
     1141 attttagcgt cgaccgcgac cattagagca agatgacttt ccttaaatat ttgtcgcatg
     1201 tccggacgca aaagcggttc ccgcttttgc gggacacgct ctgaaatgac cgccgcggag
     1261 ggtgagccgc ctgtgcctgg ggtgcgccca actagcttat gaagttcttg gtatttacgc
     1321 cggtgcagag gcgttttgag gtctattctg caacaatgcg agaccgtgcg cgccaaagtg
     1381 cgccgcaact ttgaaacccc acattacccc tggagtttgt agttcgctag ccgtgctgct
     1441 gcgttgactt cggaatgaat tcgctccgga gctgccgtga gcacgccatg atgggttttc
     1501 tggcgattat agaggatctc gcttccgtcg agtgttgcca agcgacctcc tgcctcatga
     1561 agaatgaggt cagctgcggc aatgtcccaa tctctggcat tttccgaagc aaatcccgca
     1621 tcaagcgcac ccgacgcgac attggcaatt cgcaatgcaa gcgacggaat cctcggccgc
     1681 agatcgaatt tgagcccggc ctggcgcaac ctatcggcaa gaaatcccgg tgccactacc
     1741 cttgcttcag cgttcatcgc ggcaagtttc gacaccgcga tggtcttgtc gttgagcttc
     1801 gcgccagcgc cccggacggc gatgtaggtt tctccgagca ctggggcatg gatgatgcca
     1861 agcaacgggc gccctttttc aatgagcgcg actgcgattg cccagccgct ctgccctgtc
     1921 ataaagccgc gcgtaccatc aatcggatcg ataaccaaaa tcaggtccct cgaaaggcgc
     1981 gcggggctat cctcggtttc ctcggaaagc cagccagctt ccggtaacaa aggcatcagc
     2041 tgctgcgcca aatatcgatc gacaagacaa tcggcttcgg taaccggaga cccgccggtc
     2101 ttgtgcgcta tgccagcggt cgtcggcgcc cccggacgat aatagccgag agcgagtttt
     2161 ccagcgccgc aaacaacctc cttgaaggcg gcgaccagct tgtctccgtg cgaaatcggt
     2221 tccaaaagca tgccagtgac agagaagata atcttcgctt atcctaccgc gctgcagcat
     2281 acaagcggtg ctggggtcaa ttggtcgata ccggcagcgc caatcgcgct tgcaccttga
     2341 aacaaatacc gtgcagggct ttcgcgaggg ttgtttgccc cggcccgcaa cgggtcactg
     2401 gttgctgcgg cccgcaacct tggcttccgc aaaagtcgcc attccgctgt ggcacgctac
     2461 tgccgccttg atgattgcaa atgctaaggc cgcgcccgag ccttcgccga ggcgcattct
     2521 tagatcgaac agcggctcct tgcccagcct gcgcagtacc tctttatgcg cgccttccgc
     2581 cgacacatgg gctgcaaggc aatgatcgag cgcggttttg ttgatcgcat ggagaatagc
     2641 ggcggcggcg caaacaacat agccatcgag aaccaccggg attctctgca gccgcgcggc
     2701 gaggattgcc ccagctatgg cggcgaattc gcgaccgcca agccggcgca agacctgcag
     2761 cggatcggcg agatgatcgt tgtggcgcaa ggcggcggcc ttcaccgcgg cggttttccg
     2821 ctcaaaagca tgatcatcaa ttcccgtacc gtacccaacc caatccgccg cagcaccgcc
     2881 gtagagcccg tgatagatcg ccgcagcgat cgtggtattg ccaatcccca tttcgccgag
     2941 gcacagaagg tccacccctc caggaacagc ccccattccg aaagcaaagc ttgccgccgt
     3001 ttcctcctcc tcgagccccg gttcttcggt gatgtccttg gatggaatat cgagcgccaa
     3061 atcaaaaaca accaacccta acccattgac tttgcagatt tgattgatcg cggcgccgcc
     3121 aacgctaaaa tttttcacca ttgcgcctgt gaccgaagcc ggataggcgg aaacgttctt
     3181 ttcgacaaca ccatgattgg cagcaaaaac tgcgaccatc gggcgatcta acgacggaac
     3241 cggttttcct tgccaagctg ccaaaaatgc ggggatttcc tcgagccgcc cgagggatcc
     3301 aggcggtttg gtcaactccg cttggcgcgc ttgggccgcc caacgcgatg cctccgaggc
     3361 ttggggcagg gaggcaagca actcgcgtat agaattcaac gttttcacgg aggggatcat
     3421 cgcgcgagcg tgtccagcaa aagtgggaag cagcttgcat ctggacacgc gacaaatcaa
     3481 agaaccaaga gcgtgtcgag gcaaaagtgg ggatccggtt gtttcgccgc ggcggtcgtg
     3541 tttgcgattg cggcaccgag ctttaccacc aggggccgcc tctcctcctt ccactccctc
     3601 gagaatctca cagtccgcct ctctgcagcg ccatttggcc t
//
LOCUS       DATMPU010000361         4619 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_1824837,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000361 DATMPU010000000
VERSION     DATMPU010000361.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 4619)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4619)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4619
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1824837"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..941)
                     /locus_tag="VNY06_08275"
     CDS             complement(<1..941)
                     /locus_tag="VNY06_08275"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012590173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HWX14821.1"
                     /translation="MNPLEILIHEMITENGPLSLETYMALALTHPVHGYYVSKMPLGQ
                     SADFITAPEISQMFGELIGLWCAAVWRSMGKPQPLLFVELGPGRGTLMADALRAARVA
                     PDFLSAIDIHLVETSETLQQCQRIALKDLYQAVSWHASTEDLPDGPAIIVANEFFDCL
                     PIRQFVRSTTGWHERLIGLNGDGRLCFGLAPGPEPGLTAPGEPGQVFEAGLVAARLMS
                     RLAERICLQGGALLVVDYGFDVPQHGATLQAVRSHRFADPLGDPGEADLTAHVDFCAL
                     ARAARAAGAIVHGPVPQGEWLTRLGLYERAAKLLEKA"
     gene            complement(957..1769)
                     /gene="lgt"
                     /locus_tag="VNY06_08280"
     CDS             complement(957..1769)
                     /gene="lgt"
                     /locus_tag="VNY06_08280"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012590174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HWX14822.1"
                     /translation="MPISLAFPYIDPVLVTIGPLPIRWYALAYIAGLLLGWAYARSLV
                     KRKDFWDSTQAPTPVDVDDLLVYAAMGVILGGRLGYVLFYNGAFYLAHPAEIFALWNG
                     GMSFHGGLVGTILAIYILARRKGISVLSLTDVCAAAVPIGILLGRIANFIKPELWGRP
                     TDVPWAMVFPGAGPLPRHPSQLYEAALEGVLLFFVLRLAIRLGGLRRPGLISGLFAVG
                     YGVVRIACEFFREPDPQLGFLIGGATMGMLLSLPLIAAGILVMARATGARRE"
     gene            2019..2285
                     /locus_tag="VNY06_08285"
     CDS             2019..2285
                     /locus_tag="VNY06_08285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012386441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory factor UbiK family protein"
                     /protein_id="HWX14823.1"
                     /translation="MAQNRVYEDFTRLVTDAAEMVKGVRREAETAIKSQLERLLATMD
                     VVTREEFEVVKEIAAKARDENEKLSQRVATLEAAIAAASKDCYG"
     gene            2576..3136
                     /locus_tag="VNY06_08290"
     CDS             2576..3136
                     /locus_tag="VNY06_08290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018267337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX14824.1"
                     /translation="MPDYDSDTYSTHEPRFSLRSFLVRDWPYLAMLALALFGVAYTSI
                     ARQAMTTYWIALAPFFAIFCVITRWRDVAGEQRRWQLIKTEGFHWIAVIVAMYLVFVS
                     DVRQMMSSDASALMVLTLLALGTFLAGVHIGAWRICLVGTVLAAAVPAIAWLEQATLL
                     LFLAAIVIVSVVALVLRFGRRNFMGS"
     gene            complement(3233..4291)
                     /locus_tag="VNY06_08295"
     CDS             complement(3233..4291)
                     /locus_tag="VNY06_08295"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HWX14825.1"
                     /translation="MNLQRPATLWIAALAALLVIVVLLRDILLPFLAGIALAYLLDPV
                     VGRLQRLGLNRSLAAFAILSLFIVAVGGVAVVAIPIIGTELADFIEKVPGYIGQLQAL
                     VSDPSRPWLRKLVGEGLSEAEQSAGEIARFAANWIPSLLLRVWSDSQALISAFSLLIV
                     TPIVTFYLLKDWNRLIANIDRSIPSAQRDTVQKLAEELDDTISGFLRGQGTICLILAC
                     YYALALYLIGLNHGILIGLFAGLISFIPYLGSLAGLLLSVCVAVLQFWPNWTMIPVVL
                     VIFLSGQAIADYVLAPYLVASRIHLNPVWVMFSIAAFGYLFGFVGLLLAVPLAAAVGV
                     LVRFALRQLPAGSFDTSS"
BASE COUNT         1171 a         1308 c         1279 g          861 t
ORIGIN      
        1 ctggcctttt cgagcaactt cgccgcgcgt tcgtaaagtc cgaggcgcgt tagccactcg
       61 ccttgtggca ccggaccatg gactatggcg ccggctgcgc gcgccgcccg agcaagagcg
      121 cagaaatcca catgcgcggt gaggtcagct tcgcccggat cacctagggg atcggcaaag
      181 cgatggcttc tcacggcctg caaagttgcg ccgtgctggg gaacatcgaa gccatagtct
      241 acaacaagga gcgccccgcc ttggaggcaa atgcgctcag caagtcgcga catcagccgc
      301 gccgcgacaa ggccggcctc gaagacttgg ccaggctcgc cgggcgcggt gaggccgggc
      361 tcggggcctg gcgcaaggcc aaagcacaaa cgtccgtcgc cattcagacc aatgagcctt
      421 tcgtgccatc cggtggtgct tcggacgaat tggcgtatag gcaagcagtc aaaaaattca
      481 ttggcaacaa tgatcgctgg gccgtcaggc agatcctctg tgctcgcgtg ccaactgacg
      541 gcttggtaca aatccttcaa cgcaatacgc tggcattgct gcaatgtctc gctcgtctcg
      601 acgaggtgga tatcgattgc agatagaaag tccggcgcaa cgcgcgcggc gcgtaaggcg
      661 tccgccatca gcgtgccccg ccctggcccc aattcgacaa atagaagtgg ctgaggtttt
      721 cccatcgagc gccagaccgc ggcacaccaa agacctatca attcgccaaa catctggctg
      781 atctcgggtg cagtgataaa gtcggcgctt tggccgagcg gcattttcga cacgtaatag
      841 ccgtgcacag gatgcgttag cgcgagggcc atatatgtct caagactcaa tggtccgttc
      901 tcggtgatca tttcgtgaat tagaatttca agcggattca tgggccagta tttagatcat
      961 tcccgccggg cgcctgtcgc gcgggccatg acaaggatac ctgccgcaat gagcggcaac
     1021 gacaaaagca ttcccatggt cgcgccgcca atgagaaagc cgagctgcgg atcgggttcg
     1081 cggaaaaatt cgcaagcgat tcgcacgacg ccatagccga cagcaaagag ccctgaaata
     1141 agacccggcc gccgcaaccc tcccaagcgg attgccagac ggagcacgaa gaacagcagt
     1201 accccttcga gggctgcttc ataaagttgg ctcggatggc gcgggagggg gccggcgccg
     1261 ggaaagacca tcgcccaagg cacatccgta ggccggcccc acaattccgg cttaataaag
     1321 ttagctatac ggccaagaag aatcccgatc ggtaccgcgg cggcgcagac atccgttaga
     1381 ctgagtacag aaatcccttt gcgcctggca aggatataaa ttgcgagaat ggtcccaacg
     1441 agtcccccgt gaaacgacat accgccattc caaagagcaa aaatctcggc cggatgcgcg
     1501 aggtaaaatg cgccattata aaatagcaca tagccaagcc ttccacccag aatgacgccc
     1561 attgccgcat agacgagcag atcatcgaca tcgaccggcg tgggtgcttg cgtactgtcc
     1621 caaaagtcct ttcgtttaac caagctgcgc gcataggccc agccaagcaa aaggcctgcg
     1681 atataggcca atgcatacca gcggatcggc aatggcccga tggtgacaag caccgggtcg
     1741 atataaggaa atgcaaggga gatcggcacg gcgctcaatg agtcaagata aggagtcgga
     1801 tagaatccaa ggcggcaata tccaatatgc caccactgtt tcgcagcgaa accaaggcaa
     1861 ggtcacgcac ccgaatcaaa attcggttgg ccagagacgc agccggtggc acattgaagc
     1921 gttgcacatt caagcgacaa ctccaagacg aattgccgga taaaaattgg ccagcagaaa
     1981 taccgcgtgc taaaagcgcc gtcagtctgg agtgggttat ggcacagaac cgggtatatg
     2041 aagattttac acgtctcgta accgatgccg ccgaaatggt gaaaggcgtg cgccgcgagg
     2101 ccgaaacggc gatcaagagt cagcttgaac gtttactcgc aacgatggac gtcgtcacgc
     2161 gcgaggaatt cgaagtcgtc aaggaaatag ccgcgaaggc gcgcgacgaa aacgagaaac
     2221 taagccaaag ggtggctacg ctggaggcag caattgccgc agccagcaag gactgctacg
     2281 gataggccca aaaccctggc caggaactgg gatagctgcc aaacatatca cttgggtgcc
     2341 gcgtggcaat gccagggcat aatttggcca gggcggttgc gctgcccgcc cgatgcgggc
     2401 ggctatccta aggccagcgc atcgccgccc taaccaacat gtgcaggcat ccaaactagg
     2461 caacatcatc caatctccaa gagtccggca gagctatcgg tcgcgctcct taggcgctag
     2521 aataaccaaa tcggtcgccc tgacccacca ccaaacggac tccggagtct gcaccatgcc
     2581 cgattacgac tcagatacct acagcactca tgaaccgcgc tttagcctgc gcagcttttt
     2641 ggtacgagat tggccgtacc ttgcaatgtt agcccttgcc ctattcgggg ttgcttacac
     2701 aagcatagct cgacaggcga tgacgaccta ttggatagcc cttgcgcctt tcttcgccat
     2761 tttctgcgtt atcacgcgtt ggcgagacgt cgccggcgag caacggcgct ggcagctgat
     2821 caaaaccgaa ggctttcatt ggatcgcggt tatcgttgcc atgtatttgg tcttcgtgtc
     2881 agacgtcagg caaatgatga gttcggacgc cagtgccctt atggtattga cattattagc
     2941 cctggggacg ttcctggcgg gcgtgcacat tggtgcctgg cgcatatgcc tggttggaac
     3001 cgtgctggcg gctgccgtcc ccgcaatcgc ctggctcgaa caagctactc tgctgctgtt
     3061 tttggcagcc atcgtaattg tatctgtagt ggcactggtc ttgagatttg gccggcggaa
     3121 ttttatgggc tcgtagcaga tatcgtcgtg ggattccggc gccatcgagg cattgtaacg
     3181 aaacatacgc cccgaacatg agtctgctcc attggaacga aacccccggg gcctagctcg
     3241 aagtatcaaa tgaacctgca ggcagctgcc gcaacgcaaa ccggacgagg acgccaacag
     3301 cggcagcgag gggcacagca agcaagaggc cgacaaagcc aaaaagataa ccgaaggcgg
     3361 cgatagagaa catgacccac acagggttta ggtggattcg agacgcgaca agatagggag
     3421 cgaggacata atccgcgatc gcctggccag aaaggaagat cactaatacg acagggatca
     3481 tcgtccagtt cggccaaaac tggagcaccg ccacgcaaac ggacaacaag agcccagcta
     3541 acgacccgag ataaggaatg aaactgatca gaccagcgaa aagccctatt agaattccat
     3601 ggttgagacc tatcaagtac aaggccagcg cataataaca ggcaaggatc aggcaaatgg
     3661 tcccctgccc gcgaagaaat cccgagatgg tgtcatcgag ctcctcggca agcttttgca
     3721 ccgtatcgcg ctgcgccgaa ggaatggacc ggtcaatgtt tgcgatcaga cgattccaat
     3781 ccttaagcag atagaatgtc acgatgggag tgacgataag cagggaaaat gccgaaatca
     3841 atgcttggct atccgaccat accctgagca acaggcttgg gatccagttc gcggcaaaac
     3901 gggcgatctc cccagccgat tgttccgcct cgctcaatcc ctctccgaca agcttgcgca
     3961 accaagggcg gctcggatca gacactaggg cttgaagctg tcctatgtaa cccgggacct
     4021 tttcaatgaa gtcggcgagc tccgtgccaa tgattgggat tgccacgact gcaacgccgc
     4081 caacagctac gatgaacaga ctgagaatcg caaatgcggc aaggctccgg tttagtccta
     4141 atcgttggag ccgcccgaca accgggtcga gcaaatatgc gagcgcgatg cccgcaagaa
     4201 atggcaacaa tatatcgcgc aacagcacga ctataacgag taatgctgcg agagccgcga
     4261 tccaaagtgt ggcaggccgc tgcaggttca taaaccgcat ttctgacggc aatgactatc
     4321 gcgcatgatc ctctgccgcg aatttcggcg attgattcgc ctcaaatttt gccgattgag
     4381 cgtaaatgtc aaccgaaact tgatctttgc atttggatgc acttggccct tgcccaatct
     4441 gtgaatcttt cgctgggttc cctcggagta gagcatttat ctcaaaatgc cgcggaggta
     4501 ctccgcccgg cctggaaact cgatccacct cgaaccaaaa ccttctcaat ggggacaacc
     4561 gtgctaaacc cagttgcaaa aggcctgcac ctcgtgattc ctttcagccg ctgaccaaa
//
LOCUS       DATMPU010000362         2844 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_1826570,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000362 DATMPU010000000
VERSION     DATMPU010000362.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 2844)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2844)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2844
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1826570"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(424..>2844)
                     /gene="secA"
                     /locus_tag="VNY06_08300"
     CDS             complement(424..>2844)
                     /gene="secA"
                     /locus_tag="VNY06_08300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018266558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HWX14826.1"
                     /translation="DYLARRDAEWMGSIYKFLGLTTGIIVHGLDDDERRRAYAADITY
                     GTNNEFGFDYLRDNMKYDLAQMVQRGHNYAIVDEVDSILIDEARTPLIISGPSDDRSD
                     LYIAIDKAIPRITGEDFELDEKQRTTNLTDAGNEHMEEMLRELGVPLEGSLYEAANVT
                     VVHHVNQALRAHKLFKRDKDYIVRKGEVILIDEFTGRMMPGRRFSEGLHQALEAKEHV
                     TIQPENVTLASITFQNYFRLYSKLSGMTGTAATEADEFAEIYKLDVVEIPTNKPVIRI
                     DEDDEVYRTSEEKSRAVICEIETANADFQPMLVGTTSIEKSELLAEAMRKKGYKQIDF
                     SEPEALQKLYAAARSAKPSKLFAVLNARFHEQEAYIVAEAGVPGAITIATNMAGRGTD
                     IQLGGNVEMRVAQECATLPPGAERDAKEAEIRAEVEKFRAKAIAAGGLYIIGTERHES
                     RRIDNQLRGRSGRQGDPGRSKFFLSLKDDLMRIFGSDRMESMLVKLGLKEDEAIVHPW
                     INKALEKAQQKVEARNFDIRKNILKYDNVMNDQRKVVFEQRREMMAQDSLEDMITDMR
                     HGVVDDLIDKHIPSDTYPEAWDVDGLKQSLQDSFNIALPVADWAKEEGITEDEMHERL
                     QAEADKAYAARVEKNGAEVMRYIEKQIVLQSLDHHWREHLVTLDHLRQVIGWRGMAQR
                     DPLNEYKSEAFELFGELITHLRQMTTSQLNRVEVAFEPAPPPMAPLEVSHAEASAYPD
                     RTMEAAFATARAPQAVKPGFGDSAAAAAETLVRESLERGVFGKVGRNQPCPCGSGKKY
                     KHCHGAVV"
BASE COUNT          560 a          814 c          783 g          687 t
ORIGIN      
        1 ccgccggcac gccacatcat gggtgtgtct tgggcggaat gggtgtgtct tgggcggaat
       61 gatcttgggc ggatgccatg aggggttgga acgtcagtgc tgccacaaca gctgagcagc
      121 acaataggct cgggactctg agtttcatga ccggtctccg gaccgttcgg acgcctcctt
      181 agctagttta gcggaaaggc gattgagctc gtcgcgataa atcaattggt tgctcaaatg
      241 aaggcggagc agcaccaagc ctgaaatggc gccgcggtgc tcgatttatt gcacgccacg
      301 aaacgggtag aatcggcggc tccgaccttt ggctagcctg cgcccgcttg tgtcgaaccc
      361 tgctgccctc gtctcgttca aaaggcagcc acgattcacc cctttttacc catgggctgc
      421 ggtttaaacc acggcgccgt ggcaatgttt gtatttcttc cccgagccgc agggacaagg
      481 ttgattgcgg ccgactttgc caaaaacacc ccgctccaac gactcacgga cgagagtctc
      541 ggcggcagcc gctgcggaat ccccgaaacc gggcttaacc gcttgcggcg cccttgcagt
      601 tgcaaatgcc gcttccatcg tgcggtccgg ataggccgat gcctcggcat ggctcacttc
      661 tagcggcgcc atcggaggcg gcgccggttc gaatgcgacc tcgacccgat tcaattgaga
      721 tgtcgtcatt tgccgcagat gggtgatcag ctcaccgaac agctcgaagg cctccgactt
      781 atattcgttc agcggatcgc gctgggccat gccgcgccag ccaatgacct gacgcagatg
      841 atcgagcgtc acaagatgtt cgcgccaatg atggtcgagc gactgcaaaa cgatctgctt
      901 ttcgatatag cgcatgactt cggcgccgtt cttctcaacc cgggcggcgt aagctttgtc
      961 agcctctgcc tgcagcctct catgcatctc atcctcggtg atgccttctt cctttgccca
     1021 atccgcaacg ggaagcgcga tattaaacga gtcctgaaga gattgcttca acccgtcgac
     1081 atcccaagcc tctgggtaag tgtcgcttgg gatatgcttg tcgatgagat catcgacgac
     1141 gccgtgccgc atatcggtaa tcatgtcctc aagcgagtct tgcgccatca tctcccggcg
     1201 ttgctcaaat acgaccttgc gctgatcgtt catcacattg tcatatttga gaatgttctt
     1261 gcgtatatcg aagttgcgcg cttcgacttt ctgctgcgct ttttccaggg ccttgttgat
     1321 ccaggggtgg acaatcgctt cgtcctcctt gaggccgagc ttaacaagca tggactccat
     1381 ccggtcggat ccgaagattc gcatcagatc gtctttgagc gataggaaga attttgagcg
     1441 tccagggtcg ccttgccgcc cggagcgccc gcgcagctga ttgtcgatac ggcggctctc
     1501 gtggcgttcg gtgccgataa tataaagacc ccccgcggcg atcgccttcg cgcgaaactt
     1561 ttcgacctcg gctcggattt cggcttcctt tgcatcgcgc tctgcgcccg gcggcagcgt
     1621 ggcgcattcc tgcgcgaccc gcatttcgac atttccgcca agctgaatgt cggtgccgcg
     1681 ccctgccatg ttggtggcaa tggtgatggc acctggcacg ccagcctcgg cgacgatata
     1741 ggcttcttgt tcgtgaaagc gcgcattcaa aaccgcgaaa agttttgaag gcttggccga
     1801 ccgcgcagcg gcgtagagtt tttgcagcgc ttcgggctcg gaaaaatcaa tctgcttata
     1861 acctttcttt ctcatcgcct cggcaagcaa ctccgacttt tcaatcgacg tcgtgccaac
     1921 tagcatcggc tggaaatccg cattggctgt ctcgatctcg cagattaccg cacgagattt
     1981 ctcttccgac gtgcgataga cttcatcgtc ctcatcgata cgtatcactg gcttgtttgt
     2041 gggtatctcg acgacgtcaa gcttatagat ttcggcgaat tcgtccgcct cggtcgcagc
     2101 ggttccggtc attccggaaa gcttgctgta aaggcggaaa taattctgga aggtgatcga
     2161 agcgagtgtc acattttccg gctgtatggt cacatgctct tttgcttcaa gcgcttgatg
     2221 cagcccttcc gagaaacggc ggcctggcat catccttccc gtgaactcgt caataagaat
     2281 aacctccccc ttgcggacaa tgtagtcctt gtcgcgcttg aacaatttat gtgcgcgcaa
     2341 ggcctggttc acgtggtgca caacagtgac attggcagct tcataaagcg aaccctccaa
     2401 gggcacgcca agttcccgga gcatttcctc catatgttca ttgccggcgt cggtgaggtt
     2461 cgtcgtgcgt tgcttctcgt cgagctcaaa atcctccccg gtgatgcgcg ggatggcttt
     2521 gtcgatggcg atgtaaaggt cagacctgtc gtcagacggg cctgaaatga tcagtggtgt
     2581 ccgggcctca tcgatgagga tggaatcaac ctcgtcgacg atcgcataat tgtggccgcg
     2641 ctgaaccatc tgggcgagat cgtatttcat attgtcgcgc agatagtcga agccgaattc
     2701 attgttggtg ccataggtaa tatcggcggc ataggcgcgg cggcgctcgt catcatcgag
     2761 gccatgcacg ataatcccag tcgtcaggcc gagaaattta tagatgctgc ccatccattc
     2821 ggcgtcgcgt cttgcgagat aatc
//
LOCUS       DATMPU010000363         2725 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_1826621,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000363 DATMPU010000000
VERSION     DATMPU010000363.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 2725)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2725)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2725
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1826621"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..241
                     /locus_tag="VNY06_08305"
     CDS             <1..241
                     /locus_tag="VNY06_08305"
                     /inference="COORDINATES: protein motif:HMM:NF016830.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="carbohydrate porin"
                     /protein_id="HWX14827.1"
                     /translation="QGKLYLAAGGLGGIIGDGQLPNAGPEQILEAFYRVGVFEWAHFG
                     FDYQFINHPAYNRDRGAGIDLDLTGSSAVLTRLIN"
     gene            1056..2174
                     /locus_tag="VNY06_08310"
     CDS             1056..2174
                     /locus_tag="VNY06_08310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX14828.1"
                     /translation="MLEATGRSGADEGYDARGFEIVDISSSVPLEATEGDDEEAIAAP
                     TTDRLWLIQCKREREITPTKIRQHLSKISEDEAKTLYGIIFAAACDFSKRTRDEALSW
                     GRTHGISEVHIWGKGEIEDQLFQPKNDNLLFAYFGISLQIRRRSIRTALRATLAMKRK
                     AMRALGDRWKDNKTILLRDPEDTRYPNIDQPNGSLENHNWRLTEFRGHDIGGIKIAWR
                     RYFAYLDTDGKKWDIADIFNIEGHLPQQNPWNDPREIERLYRLEDEIRKFWEGNIPEE
                     CRGQLYVEVLLHYHEILEIDEKGDPLVGHPHVFTSFTNNRPPFAGFLGELTIPPFYIG
                     GPNNQGIKTRDMTKERKLWSPEPNDRVRIFPEAFRKGK"
     gene            2331..>2725
                     /locus_tag="VNY06_08315"
     CDS             2331..>2725
                     /locus_tag="VNY06_08315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX14829.1"
                     /translation="MLERVVAIKNIGRFRNSIASPNPAFAKHTVMFGANAYGKTTFCA
                     VVRSVQSGNVAPLLGRQTLGVSAIPDVDLLFTDGNRRLQDAVWSAVAPQISVFDGVFV
                     SENVHSGDVVDVPHRRNLFRVIVGRAGVAL"
BASE COUNT          635 a          729 c          779 g          582 t
ORIGIN      
        1 tcagggcaag ctttatctcg ccgccggcgg gctcggcggc atcatcggcg acggccaatt
       61 gcccaacgct ggcccggaac aaatcctcga agccttctac cgcgtcggcg tgttcgaatg
      121 ggcgcatttc ggtttcgact accagttcat caaccacccg gcgtataacc gcgaccgcgg
      181 ggccggtatc gatcttgacc ttacaggttc atctgcagta ttgacacggc tcatcaacta
      241 aagactgtgt aacgcgcagg gcatcggcgc cttcttgcgg gtgatgaacg gcgccgggcg
      301 atcgaaatct tagactagca cttgcttcgt cgcccgcagg gtcgagaggc gcattgcggt
      361 gccggcgtta gcggaacaat cacttagctc gcgcagaaat ctgatgcgaa tgtccgcttt
      421 caccggaccc cgctgatcct cgggccgaag atggacggcg gctatggggc agaaccggtc
      481 gatgcctctg gaagagccga gtgcgcagca tatcggctgc attatccgcg cgctgcggca
      541 ttggcgagaa gtgcgttggt tgctcgacat ttcgggctcg cgcgccaatc gcgtcgcgcc
      601 tgatgccccg tcgaggaagc tcgagccagg tcgagaggcc tggctcccct gtcgtttatc
      661 gcggggcggc gggtgatcgg ccggccgccg cgtcggcgag aggaggggcc cggtcagaga
      721 cggccgggct tttcgcttga aaatcgctta taatcggtgt gtgcaaaccg ctgccgcctg
      781 gtcccgctca ccaccgcatc gaaatcacgg ctgcagcatc gcgaaccagc acccgaggca
      841 attcgtcgcc gtggcgagct cggcgagccg ctcgtcgaca ttgcacgcac ctacaacgtg
      901 aaccgcagca cgatttcccg gctcacggca tgatgaaatc aagaatgccg gcaatcacca
      961 caaagacgct aaacccactg ccattcgatg ctcttgagcc aaagcgcttt gaggacctta
     1021 ttcgtcagct tgcctacgac tttcgaactt ggcgcatgct tgaagcaacc gggcgcagcg
     1081 gagccgacga aggctatgac gcgcgagggt tcgagatcgt cgatatctct tcgagcgtgc
     1141 ctttggaggc aactgagggt gatgatgaag aagcgatagc cgcgccgaca actgataggc
     1201 tttggctcat acaatgtaag cgcgagcgcg agattacccc gacaaaaata cgacagcatc
     1261 tttcgaaaat atccgaagat gaagccaaga ccctgtatgg cattatattc gcggcggcgt
     1321 gtgatttttc caagaggaca cgggatgaag ctctctcttg gggccgcact cacggcatat
     1381 ccgaggtgca tatttgggga aagggagaga ttgaagacca attatttcaa ccaaaaaatg
     1441 acaatcttct ttttgcatat ttcgggatct cgcttcagat caggaggcgt tcgattcgca
     1501 cggccctgcg cgcgacgctc gcaatgaagc gcaaagcgat gcgggctctt ggcgatagat
     1561 ggaaggataa caagacgatt ttactccgcg atccggaaga cacacggtat ccgaacatag
     1621 atcaaccgaa tggttcactt gaaaaccaca actggcgttt aacggaattt cgcgggcacg
     1681 acatcggtgg cataaagata gcatggcggc gatattttgc ctatcttgat accgatggca
     1741 aaaaatggga catcgccgat attttcaata ttgagggtca tttacctcaa caaaatccat
     1801 ggaacgatcc aagagaaatt gaacgtttgt accgtcttga ggatgaaatt cgcaagttct
     1861 gggagggcaa tatacccgag gagtgccgcg ggcaactcta tgttgaagtc ttattacatt
     1921 atcacgagat tttagaaatc gatgaaaaag gtgatccgct tgtaggccat cctcatgtgt
     1981 tcacgtcgtt cacaaataat agaccgcctt ttgccgggtt ccttggcgaa ttgacaatac
     2041 caccattcta catcggtggt ccaaataatc agggaattaa gacaagggat atgacgaaag
     2101 agagaaaact atggagtccg gaaccgaatg acagggtacg gatatttccg gaggccttcc
     2161 gaaaggggaa gtgaaattcc gatagctccg cccgatggca acggcgagcg ggagtcccgc
     2221 gaacgccccg cggccgagcg agacacagcg cagcgttgcc cgcagctaca acgtcagtca
     2281 ggcgacgatt tcaaggctcg cagcatgatc ccgcggaggg acaggccgaa atgctagagc
     2341 gcgtcgtcgc catcaagaat attgggcggt ttcgcaattc gatcgcctcc ccaaatcctg
     2401 cgtttgccaa acacactgtg atgtttggcg cgaacgctta tggaaagacg acgttttgtg
     2461 cggtcgtacg ctcggttcaa tcaggcaacg tcgctcccct tcttggccgg cagactcttg
     2521 gagtttcggc tattccagat gttgatcttc ttttcacaga tggcaatcga cgactgcaag
     2581 atgcagtttg gtcggcagta gccccgcaaa tttccgtttt tgatggggtg ttcgtctcgg
     2641 aaaacgtgca ctccggggat gttgtcgacg tgccgcacag gcgcaacctt ttccgtgtga
     2701 ttgtcgggcg agccggtgtg gcact
//
LOCUS       DATMPU010000364         3153 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_919865,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000364 DATMPU010000000
VERSION     DATMPU010000364.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 3153)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3153)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3153
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_919865"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1..110
                     /locus_tag="VNY06_08320"
                     /pseudo
     CDS             1..110
                     /locus_tag="VNY06_08320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008526372.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family oxidoreductase"
     gene            475..774
                     /locus_tag="VNY06_08325"
     CDS             475..774
                     /locus_tag="VNY06_08325"
                     /inference="COORDINATES: protein motif:HMM:NF026066.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX14830.1"
                     /translation="MMPLDASAVAKLLHEFGQRTALRGGNPYRARAYSRAAENLMVLT
                     VPLEVVVAQDRLKEIPDVGDAIADIVTKLHQKGGHPALDAMRKEIPAGALMFPLD"
     gene            1299..1829
                     /locus_tag="VNY06_08330"
     CDS             1299..1829
                     /locus_tag="VNY06_08330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015821703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-starvation-inducible PsiE family
                     protein"
                     /protein_id="HWX14831.1"
                     /translation="MGNQVGAERKARGGLRFVLLRETAEAWPSLGIYQRFEQAVALVL
                     TFLISGVIVVAIAQLVLVEAQDLFLNPLARVDVTIFQSIFGMIMTVLIALEFNHTIMS
                     ILHRKDSIVQLRTVILIALLAMARKFIIIDFTGLEPLAVFGLASAVLALGLVYWLVRE
                     EDRREAEHEANSTAAR"
     gene            1889..2074
                     /locus_tag="VNY06_08335"
                     /pseudo
     CDS             1889..2074
                     /locus_tag="VNY06_08335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009489216.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
     gene            complement(2410..2679)
                     /locus_tag="VNY06_08340"
     CDS             complement(2410..2679)
                     /locus_tag="VNY06_08340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX14832.1"
                     /translation="MAQFVSAIDIGNGLPDVLEMGATVRYWRNLGNGHWDLPHEMRDA
                     PAGLGLSDPGVQLIDADGDGRRCGFWEDAARGSEIDVSVANCVEL"
BASE COUNT          681 a          873 c          917 g          682 t
ORIGIN      
        1 caccctcctc gaaacaggcg agcacgaaag ccgccgtgcg ctcgttcgcg cgaacatgga
       61 caacggacct aaaggatcgc cgcattcgcg tgaacgcagt gagcccaggc gcctgcgcaa
      121 aaggtcgcgg aaggcttcct cggccagctc ctggaaggcc atcatgcggt cgcgggcaag
      181 caagtcgacg gcctgccacg tttcatcgtc gaactggacc cgctttccgg gcatcagccg
      241 ccacctgtcg aacctgaaga aatggcaccc cgtcccgctt ggcgtcaatg ccatgcttcc
      301 attgacgata agtttcgctc attggtccgg gcttctgatc gcagcaaaag gtcatacagc
      361 gtcgcgatgt aacgtccgaa atacggccta agttccgcgc cagcacgttg gcgagtgcgg
      421 cgcgggacca aagccttggc ggcgcgttta tcaggaaatt gcacttggaa ttaaatgatg
      481 ccgctcgatg cttccgcggt tgccaagctg ctgcacgaat tcggccagcg cacggccctg
      541 cgcgggggca atccctatcg cgcgcgggcg tatagccgtg cggccgaaaa cttgatggtc
      601 ttgacggtgc ccctcgaggt ggtggtggcg caggatcgcc tcaaggagat cccagacgta
      661 ggcgacgcca tcgcggacat tgtcaccaag ctgcatcaga agggtggcca ccccgcgcta
      721 gacgccatgc ggaaagagat tccagcggga gcgctcatgt ttccactgga ttgaatatac
      781 gacggctctc cgtgcgcgag ggctttttcc gcaacagacg ctgacgccga tccgaaatgg
      841 ccggttgcgc gatctcaaaa agacctgaag gtggcctcgg tcactcttta ccgttacgtc
      901 gcatcagggg cgaacagcaa aggaccaaat tctgggccga ttccaagatc accgaagcca
      961 atttgcttat gataaccaaa caaggatttc gttttattag aaaattacaa tggcaaagtt
     1021 acgcttggaa ctcgttcatg tgtaatgcgc gctcgcgcgc cggtcgtcga tccgtggcgg
     1081 tgaggtttca tgaatgcgca cctagtcaca accagtccga tatttgtcaa tacggcgcgg
     1141 aacgtgataa atcgcgaggg gttattggac agggcggtca gctttaccaa agcagccaag
     1201 taggaccaat ctgagagttc gatgatctgg ggaatgttgg ttgaatagtc ttttagtccc
     1261 gtcgcccggc gggcgctcac aatgggattt aatgatccat gggcaatcag gtcggagctg
     1321 aacgaaaagc acgcggcgga ctccgctttg tcctacttcg ggagacggcg gaagcctggc
     1381 ccagcctcgg aatttatcaa cgcttcgagc aagcggtggc gctggtcctg accttcctga
     1441 tcagcggcgt catcgtcgtc gccatcgcgc aactggttct ggtggaggcc caggaccttt
     1501 tcttgaatcc tctcgcgcgt gtagatgtca cgatcttcca atccatattc ggcatgatca
     1561 tgaccgtgct gatcgcattg gaatttaacc acaccataat gagcatcctg caccgcaagg
     1621 acagcattgt tcagctgagg accgtgattc tcatagcttt gctcgcaatg gcgaggaaat
     1681 tcatcattat cgacttcacc ggcctcgagc cgctcgcggt cttcgggcta gcctctgctg
     1741 ttttggcgct cgggttggtc tactggctgg tgcgggagga ggaccggaga gaggccgaac
     1801 atgaggcgaa ttcgaccgcc gcccggtgaa agctttgcgg cggccgacgg aaaatctagg
     1861 ataggtccac gaaaagcaag gataagagcc acttcccaaa atcgggccat ggcgggatcg
     1921 gctggatcgc ctgctgctgg cgaacgaaga aaaatcagca cgcgagcgac tgacgcttgt
     1981 ccgactattc gaagagctgc gcggatttgg ttatgacggg agctacgatg ccgttcgccg
     2041 ctacgcgata agctggcgca aggagcgcag cgcgtcatgt tagcctcctt cgaaatgttt
     2101 cctgatggtc gcgcggggaa tgttcctgcc ccattagcca aaatgcagga gttgctgtac
     2161 ttgttccatc cttgttcaat gcctgggagg ccttcgctta agcagtgcct gttgaaaaca
     2221 gtgcattgat ctcatccgca tagggtcttc gtgaaccgga gttttcaaag attgcaaata
     2281 cattcactcc gtttcttaca ccgaagagga acagcaacca tatccgacag atgacagaac
     2341 tcatccggct cggggacaac ggaggccgtg gtaggcaaga cgggtttcga tcacgcgctt
     2401 ccatgcggat catagctcaa cacagttcgc aactgataca tcgatctcgc tccctcgcgc
     2461 ggcatcttcc caaaatccac acctgcgccc gtcaccatcg gcatcgatca gctgaacgcc
     2521 cggatccgaa agtcccagtc cggcgggcgc gtcgcgcatt tcatgtggca ggtcccaatg
     2581 cccattgccg agattgcgcc agtaacgcac cgtcgcgccc atttcgagga catcgggcag
     2641 gccattgccg atgtcaatgg cggagacaaa ttgtgccaaa tggcggcgag aaagtgtacc
     2701 agtcgggtta agagagaagg ctttgaagcc ccggtgttta tttttgttct tttgtactga
     2761 taaaaaccat tgatctggcc cgccgaatag gcgctcagaa tcgtcgcgga cggcccaaaa
     2821 ttacaaaatt ccctgttaat ttccctgata gcagggaatt tgaggtggag accggttcgc
     2881 agccgactgt ctcgtcagcc agggagtcgg gtctccgagg ttttatttcc cggggtgtga
     2941 gaatccgcga cattcccgcg ggtttggctg gcgcgcctca gtctctgacc ggcgattttt
     3001 ggaatttcgg tccttgaggg gcgggttttg ggcgccggtc tctgggcgcc attttccaat
     3061 ttccatttcg gtggggcaga gaccggttcg attgcagaca gagaccggtt cgcagagcgc
     3121 ccttggggga ttggtcggcg ctgacgccac tgc
//
LOCUS       DATMPU010000365         3207 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_4614402,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000365 DATMPU010000000
VERSION     DATMPU010000365.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 3207)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3207)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3207
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4614402"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..118)
                     /locus_tag="VNY06_08345"
     CDS             complement(<1..118)
                     /locus_tag="VNY06_08345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014129213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HWX14833.1"
                     /translation="MRATDRLSETFSALADPTRRAILARLALGEASVNELAEP"
     gene            587..1344
                     /locus_tag="VNY06_08350"
     CDS             join(587..917,917..1344)
                     /locus_tag="VNY06_08350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611908.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HWX14834.1"
                     /translation="MIRLFLLSERQMARICPYFPLSHGVPRVDDRRVVSGIVYVIKNG
                     LQWKDAPRDYGPHKTLYNRFIRWSRLGVFDRIFAALASGSKPERLMIDATHLKAHRTA
                     ASLLKKGLFPRRIGRTKGGLNSKLHAVCDGEGKPLVLLLSEGQMSDHKGARLILDALP
                     PAKLLLADRGYDSNWFRDALARKGCKPCIPSTKSRKIPLPYDKTLYKQRHKIEIMFSR
                     LKDWRRIATRYDRCAHTFFSSICIAASLAFYLNQ"
     gene            1858..2337
                     /locus_tag="VNY06_08355"
     CDS             1858..2337
                     /locus_tag="VNY06_08355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019966583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion associated locus B family protein"
                     /protein_id="HWX14835.1"
                     /translation="MTLIGIWLLANGSGAVCQGVVKSKHGDWQLRCETPPGAANEQCA
                     LLQSVAAEDRPNINLMVIVLKTADGKSRLLRVIAPLGVLLPSGLGLKIDDMDVGRAGF
                     VRCLPTGCVAEVVMEEKLIEQMKTGQKATFIIFQTPEEGIGIPLAMAGFKEGFDALP"
     gene            2324..2998
                     /locus_tag="VNY06_08360"
     CDS             2324..2998
                     /locus_tag="VNY06_08360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX14836.1"
                     /translation="MRCRELGRNSRCWLVPIHLTKSVLAAPLVLGLVGCGGHLGTFDT
                     PQTQGGGSQVLAMLLGFKNNDAPAAPGTQVRHIFCPEVVVLEGTAASQTYAGTPPSSA
                     NLRYQYALDDTARECTLEGDQLAIKIGIAGKVLLGPAGSPGSFSVPVRMAVLREHDNQ
                     PIVSKLYRAAVTVAAGETRADFTVVSEPLRVPFIKDHAEDDYTIKVGIDEGASADKPA
                     GNHVKP"
     regulatory      3108..3205
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
BASE COUNT          714 a          941 c          921 g          631 t
ORIGIN      
        1 agggctctgc cagctcgttc acagacgctt cgcccaacgc cagtcgcgcg aggatcgcgc
       61 ggcgggtggg atcggccagc gccgagaagg tttcagaaag gcggtctgtg gcacgcattt
      121 tttattaaac cagtgggtta tttatctgat aggttaaata ctgacgaaaa atcgatccgt
      181 caagcggccg tcgatcacaa tttttttcag cggtacccgg cgcggatccg gcaatttccc
      241 ggaggggcac cgttccacga tgtgtcggtt caaccgcgcg cctttggaag ccgaatttct
      301 gtcggctcca ttggcgtatg tgttaaacca attgcagcag ccaacccgcg aaccatgcgg
      361 agcgacagat gtgccttgcg aaaggcgtca gcttcgcaag ctggcagaac cgtgccgaat
      421 gcccagtttc ttccaatagg cgtggtccaa cttagtcatc acgccagaat caaatagctg
      481 tcacgacggc attgacacgt tagcgaccaa cgagccaggg catgtgctag gctcaggacc
      541 tattaactga aaggtgattc aaggtctcca tggaaggagg cttgagatga ttcgtttgtt
      601 tttgctgagc gagcgccaga tggcgcggat ttgtccttat tttccgttgt ctcacggcgt
      661 gccgcgtgtc gacgaccgtc gcgttgttag cggtattgtc tatgtgatca agaacgggct
      721 gcaatggaaa gacgcgccgc gcgactatgg tccgcataag acgctttaca accgtttcat
      781 ccgctggagc aggctcggcg tgtttgaccg gattttcgcg gcactcgcca gtggatcgaa
      841 gccagaacgg ctcatgattg acgccaccca tctaaaggca caccggaccg cggcgagcct
      901 cttgaaaaag gggctgttcc ccgccgcatt gggcgcacca aaggcggcct gaactcgaag
      961 cttcacgctg tctgcgatgg tgagggcaag cccctcgtcc tgctgctcag cgagggccaa
     1021 atgagcgatc acaagggcgc gcggctgatc ctcgatgccc tgccgccagc caagctgctc
     1081 cttgccgatc gaggttacga tagcaactgg ttcagggatg ccctcgcccg aaaaggctgc
     1141 aagccctgca taccctccac caaaagccgg aaaatcccgc tgccctatga caagacgctc
     1201 tacaaacagc gccacaagat cgagattatg ttctcacgcc taaaagattg gcggcgcatc
     1261 gccacccgtt acgaccgttg cgcccacacc ttcttctcaa gcatctgtat cgctgcatcg
     1321 ctcgccttct acctcaatca atgagtcctg agcctaggtc acaaaaacgg gctgtttatg
     1381 gagttatttc ttaggacgag gtgaatttca ttagtagaat taaggctaaa tactagaatt
     1441 aaggctaaaa attacgacac ctacgcctaa ggactataac gaaaccgtgc agcgcctagc
     1501 cgacgcccca tgtccatcag ctcaaaccat gttcctcagg tcgacgatct agccgcaggg
     1561 gcctggacaa ggggtccccg cctcgcaatg cctccaaggc gccagccgcc gcattcccgg
     1621 caacaaatcc gtctctggcc gaacggaaca caattgcaat cgccatcgca caaaagctgc
     1681 cggacggttc acaatcgtga cggcacgcat taaactcaag cacgcccacc cgtcagttcg
     1741 cttgaatcgg gccatgatcg ggccggtagc ggctcaaatg tctcaaatag acaggaacct
     1801 cccgtggcgc gcgccatcga atcaatcaaa acaacgggcg cgcggcacct tttcgggctg
     1861 acgctcattg gaatttggct cttggcgaac gggtcgggcg ctgtttgcca aggcgtcgtg
     1921 aaatccaagc atggcgattg gcaactgcgc tgcgagacgc ctcctggcgc cgccaacgag
     1981 caatgcgcgc tgttgcagag cgtcgccgcg gaagaccggc caaacatcaa tctgatggtc
     2041 attgttctca agacagcgga tgggaagagc cgccttttgc gagtcattgc accgcttgga
     2101 gtgcttcttc cgtccggatt ggggcttaag atcgacgata tggatgtcgg acgcgccggt
     2161 ttcgtccgct gccttccgac cggatgcgtg gcggaggtgg tcatggagga aaaattgatc
     2221 gagcaaatga aaaccggcca gaaggccacc ttcatcattt ttcagacccc ggaagaagga
     2281 atcggcatac cgctcgccat ggccgggttt aaggaagggt tcgatgcgtt gccgtgaatt
     2341 gggccggaat tcaaggtgct ggctcgtgcc gatccaccta acaaaatccg tcctggccgc
     2401 gccgctggtg ttgggactcg ttggctgcgg cggccatctt ggcactttcg atacgcccca
     2461 aacccaaggg ggcggctcgc aggtgctcgc catgttgctg ggatttaaga ataacgacgc
     2521 gccggcggcg cctggaacac aggtgcggca tatcttctgc ccggaggtcg ttgtgctcga
     2581 aggcaccgcg gcaagccaga cttacgctgg aacgccgcct tcgagcgcca atctgcgtta
     2641 tcaatatgcg ctcgacgata cggcgcgcga atgcacgctc gaaggcgatc aactcgcgat
     2701 caagatcgga atcgcgggca aagtgctgct aggacccgcg ggcagccccg gcagttttag
     2761 cgtgccggtg cgcatggccg ttcttcgcga acacgataat cagccgattg taagcaagct
     2821 ttatcgcgcc gccgtgacgg tggccgctgg cgagacccga gcggatttca cggtcgtctc
     2881 cgaaccgctc cgcgtgccat tcataaagga ccacgccgaa gacgattata cgataaaggt
     2941 cggcatcgac gagggcgcga gcgcggataa gcctgccggc aatcacgtca aaccttgacc
     3001 gcgcgaacgg cgaggccaac gatccggccg cctgcggctg tgcgaacttg cgccaaccgc
     3061 attgacaaaa aggcttcatg cgctaagtct taaaatgtca gatcacaaga accccagcgg
     3121 aagagaccgg ctcgcgcgtg ttgcggcgag ctggcgccga aggcgcaacc gccccggaaa
     3181 cgctcaggcc cacggaccgc tcggggg
//
LOCUS       DATMPU010000366         8233 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_4617313,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000366 DATMPU010000000
VERSION     DATMPU010000366.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 8233)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8233)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8233
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4617313"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            601..8013
                     /locus_tag="VNY06_08365"
     CDS             601..8013
                     /locus_tag="VNY06_08365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019904552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HWX14837.1"
                     /translation="MDIAIIGRACRLPGANSADELWNVLNEGRCTVSRIPPDRWPQER
                     LMHPRPKERGRSYTWAAGVLDDIWGFDPAVFGISPREAEQMDPQQRLLLELTWEALED
                     AGIRPSALAGTETGVFVGGSTLDYGNLRLHDPAGADAYFATGNTLAVLSNRISYVFDL
                     HGSSFTIDTACSSSFVALDAAVTAIQTGRIDTAIVGGVNILASPFGFISFSQATMLSQ
                     TGLCRAFSAKADGYVRAEGGVVLILRAAGAARVSGDRSHAMIAGSQVNSDGRTSGISL
                     PSKAHQAALLERAYSKHGIRPDALAFIEAHGTGTRVGDPVETAAIGEVLGRRRNRPLP
                     IGSIKTNIGHTEAASGLAGLFKAMLALEHNELPRSLHFDDPNPDIPFADLNVTVCADP
                     VPLPPNGHARYAGISSLGFGGTNAHVILKDPPPRERTGTASAPRFLLLSAHSRESLAK
                     LAQQYSALLSHTGTEEIQRVIAATGHRRELLSERLVIPLDEHAALIETLDEAACGGEI
                     APAASTNTAVDRAAPTAFVFSGNGGQWPGMGRAAFETNPVFRDQFSKIDRIFRSFAQW
                     SLTEMMLSSSIASKLEKTSIAQPLIFAIQVATVRCLARLGIAPDMVLGHSMGEIAAAE
                     VAGILDLESAVRVIYFRSRHQEIGRDAGGMAVVFGPLEVVEALVSEIPELTIAAHNSP
                     RSFTIAGPFSALDQAAKRGRAYKVRMRRLDLVYPFHSELMKPAEVPLIAGLECLQPKP
                     SQVCFLSTVDGGIVEGPSLGARYWWRNVREPVRFMEGVQLAVRLGARVFIEIGPSPVL
                     VADIRKITEESASSVAALCVLDQKAASTDPFCQAAAQAISLGARINAKVAFGDDPGPA
                     SNLPAYPWRRKPYRLEETPESTGWMRVRPSHPLIGARLSPDLLEWRGQLDPILIPALA
                     DHRIDGQVLLPGAAFAEMALAVTKDWLGTDTATIKDLEIAQPMLFSANVSREILCRAS
                     PGTATIEIMSRARLSDSPWTVHAKAKVIFRPAPSVANETIPDVTGDIVASADLYRIAR
                     HSGLEFGPAYRQVSAAARTGQTSIVVALLAADLSPQYGLDPARLDSCFHGLILLFSDK
                     VSSGRQAAYLPVRFGTIRLEKPGAVIARARIDVRRHDARAIVADFTLVDADGTLIAQL
                     QGARYQAARRDSLDLTSQSLIQVAILADEPTAIRRKPALSLDTLAMAVVPETPTASES
                     LPPDVILIEGWATAAAHRFIRALAAGGSIDPERLIAKGRLPPGWRVWLYKLLLALEKS
                     GLVAAGPEQFVLAGNNTLPDPTQILQNIAAEHPRRSAELLLAARCNAIMESLTRGDEP
                     APIAQSVFESFETGSVSVAAAADLLASLLRPTIEAWPRDRALRILQIGHGPLSRHAVA
                     LTQSKEARLTIHDPNPLRLERARLAFAGKPCISFADSLEQVPKSSADLVIAADALQRL
                     APGKEQLARLVETMAPGGLIAAIEPAPSLFRDLVLGLREDPSAATKHSDDGFLSQPDW
                     LAQFSALPLRGISVKNVGTEAGPARFIMGERPVFAESRTLPTKVLIVGQEDPHQFETA
                     SALSNRLEDAGVECATSRERPFDRLSLADLETIVFLAPDASAGGVTAATIAQRCLALK
                     NWAAELGKNKHQLWVECPGALRTGPGTPDPVQTSLWSFARTFANEYQTLDVRRVDTHP
                     SMPAPVRARRLADALLSGTDETELLLDSNSTRVVRMQSRNALNSRGIERPAPASRLEK
                     GDGTGLDRIRWVPIDRQSPRPGQVEIAVTATGLNFRDVMWGLSVLPDEMLEDGLAGPA
                     LGLECAGRIVATGENVDDLKPGDAVVAVAGRAFATHVTVDSNMVAKVPDGLPLEAAVT
                     IPVAFLTAYYALINCAQLKPGQWVLIHGGAGGVGLAALQIARWRGARVIATASSPEKR
                     DLVAALGAEHVFDARANAFADDVRRVTGKGVDVVLNSLSGEAMELSVGLLQPFGRFVE
                     LGKRDFFSNTHIGLRPFRNNLTYFGVDLDQLMLAQPDVSRQLFREVLELVAQGEFSPL
                     PYRSFRAREFIDAMRLMQQSGHVGKIVISPPEPGEIGIASNGVFTPAREKTHLITGGC
                     GGFGLATACWLAEHGARHLVLAGRSGASSSEAQETVADLRASGITVHVEALDVADTDE
                     VEALFKRIARSMPPLSGVIHAAAVLDDALIANLDAEKLKKVLAPKVTGAENLDRYTHD
                     LPLDYFVLFSSATTAIGNPGQGNYVAANGFLEGLARHRRAAGLPALAIAWGGIEDVGL
                     LARNRLVKDMLGTRAGVKTMAARDALNLMAEALANPSLEPSEAVLVISEMNWSTARAH
                     LPLLQSPTFSGLARTNEAAAAENRAKLDIRALAATVSLEEARKQIVGVIVEEIAHILR
                     LPQETVTKGKPLADIGVDSLMAVELAAALEDRLTLEVPLSASASGFNVNELADHILGL
                     CISPTSEDESIAQGLAERHLGKGIDPAALESLTALVEERSRDLTQILR"
BASE COUNT         1651 a         2458 c         2368 g         1756 t
ORIGIN      
        1 ggccgaggaa ttcctcctgc cgccgaaaaa tgcaaccaac taagagagta ggattccatg
       61 caggctattc ctggatcgac cgaagtgttt ctttgcagga tggttccaga gtttatagta
      121 cgtttagagc cgcacgttgc atgaacggta gaccttaatg ggaccgacta gacgtttcta
      181 aatacgaaag aacttcacta tgacaaggtt tataaggaaa cagcctcaat ttgtgactat
      241 catttcatga cacgtataat atatatattt ataattatat attacttccc ccaatactta
      301 ttcgttgaac gtgcagcctc ttgagtttta taagcccaga gctcccgagt agaaacaaga
      361 aacaccgtta aaagaacgaa atattatttg attcttattt atgacatttt cccggcggat
      421 tagctccccg agctctagcc aaagggcgtg ggcgtcgata tgacaaatta acatatcgca
      481 tggggtcttc gcgattcgcc ttgcgccgcc gcagcctcta gctgaatcgc gctttataac
      541 ttgggcagga ttatgagaga tcgtcaaagt ggccgcccga agacgatgaa tttgcaaggt
      601 atggacatcg caatcattgg gcgggcgtgc cggctgcctg gcgccaactc cgccgacgaa
      661 ctttggaatg tgctcaacga agggcgatgc acggtatccc gcataccgcc agatagatgg
      721 cctcaagaac ggctgatgca cccgcggccc aaagagcgtg gccgcagcta cacatgggcc
      781 gcgggtgttc tcgacgatat ttgggggttc gaccccgcgg tcttcggaat ttccccgcgc
      841 gaagccgaac aaatggatcc tcagcagcgg ctgctgcttg aactcacatg ggaagctttg
      901 gaagacgccg gaattcgccc ctccgctttg gccggaacgg agactggtgt gttcgtgggc
      961 ggatcgacgc tcgattatgg caacttgcgg ctccatgacc cggccggcgc ggacgcctac
     1021 ttcgccaccg gtaatacttt ggcggttcta tcaaaccgaa tttcctacgt cttcgatttg
     1081 catggatcga gcttcacgat tgatacagcc tgctcttcgt cgttcgtggc gctcgatgcc
     1141 gcggttacgg cgatccaaac cggcagaatt gacacggcaa tcgtgggcgg cgtgaacata
     1201 ctggcgagcc ccttcggctt catcagtttc tcgcaagcga cgatgttgtc ccaaacaggg
     1261 ctttgccggg cgttctcggc gaaggccgac ggttatgtgc gtgccgaagg cggcgtcgtg
     1321 ttgatcctgc gcgcggcagg cgccgcgcgg gttagcggag accgaagtca cgcaatgatc
     1381 gctggctcgc aagtcaactc cgatggccgt acaagcggca tctcactgcc atcgaaggct
     1441 caccaggcgg cactgcttga gcgggcatat tcgaagcatg gtattcgccc ggacgcgctc
     1501 gccttcatcg aggcgcatgg cacgggaaca agggtcggtg acccggtcga gaccgccgcg
     1561 atcggcgagg ttttggggcg gcgccgcaac cgacctctgc caataggctc gatcaagaca
     1621 aatattggcc atactgaggc cgcctccggt ctcgcgggct tgttcaaggc gatgctggcg
     1681 ctcgaacaca acgaactgcc ccgctccctg cattttgatg accccaatcc cgatatcccc
     1741 ttcgccgatc tgaacgtcac tgtgtgcgcc gatcctgttc ctctgccacc caatgggcac
     1801 gcccgctatg cgggcatcag ttccttgggc ttcggcggca ccaacgcgca tgtgattctc
     1861 aaggaccccc ccccccggga gcggacaggg acagcaagcg cgccccgctt cttgcttttg
     1921 tcggcgcata gccgggaaag cctcgcaaag ctcgcgcaac aatattctgc tcttctctcc
     1981 cataccggca ctgaggaaat tcagcgcgtg atcgcggcta ccggccaccg gcgtgaactc
     2041 ctttccgaaa gattggtaat ccctcttgac gagcatgcgg cacttatcga gaccttggac
     2101 gaagctgctt gcgggggaga gatagctccg gcggcaagca cgaacacggc agtcgaccgg
     2161 gccgctccca ctgcctttgt cttctcaggc aatggcggcc aatggccagg catgggccgg
     2221 gcggcttttg aaacgaatcc ggttttccgc gatcagtttt caaagatcga ccgcattttc
     2281 cgcagctttg cgcaatggtc cctgaccgag atgatgttga gttccagcat cgcctcgaag
     2341 ctcgagaaaa ccagcatcgc gcagcccctt attttcgcca ttcaagtggc gactgttcgc
     2401 tgcctcgcgc gtctcggaat cgcgccggat atggtgcttg gtcacagcat gggtgagatt
     2461 gctgccgctg aagtagcagg gattcttgac ctcgagagcg ctgttcgggt catttatttt
     2521 cgaagccgtc atcaggaaat cggccgggat gccggcggca tggctgttgt ttttgggccc
     2581 ttggaggtgg ttgaggccct cgtcagcgag atacctgagc tgacgatcgc agcacataat
     2641 tcgccgcgca gtttcacaat cgccggacca ttttcggccc tggatcaagc cgccaaaagg
     2701 ggccgcgcat ataaagtgcg tatgcgccgc ctcgacctag tgtacccgtt ccacagcgaa
     2761 ctcatgaaac ccgccgaggt gcccctcatc gccggactcg aatgccttca accgaagccc
     2821 agccaggtgt gtttcctgtc gaccgtagac ggcggcattg ttgagggccc gagcctaggc
     2881 gccagatatt ggtggcggaa cgttcgcgaa ccggtgcgtt tcatggaagg cgttcagctt
     2941 gcggttcggc taggggcacg cgtgttcatt gaaatcgggc caagcccggt gctcgttgcc
     3001 gatattcgaa agatcaccga agaatcggct tcgtctgtcg ccgccctgtg cgtgctcgac
     3061 cagaaggcag cttcgaccga tccattctgc caagcggcgg cgcaagccat ctccctcggg
     3121 gcacggatca acgcgaaggt tgcttttggc gacgatcctg gccccgcgtc caacctgcct
     3181 gcttatccct ggaggcggaa gccatatcgc ttggaggaaa ctcctgagtc gaccggatgg
     3241 atgcgcgtgc ggccgtcgca tcctctgatc ggcgcacgcc tatcgcctga cttactcgag
     3301 tggcgcgggc agctcgatcc gatcctcatt cccgccctgg cagatcaccg gatcgatggt
     3361 caagtgctgc tccccggggc cgccttcgct gaaatggcgc ttgcggtaac caaagattgg
     3421 cttggcacgg acaccgcaac gatcaaagac ttggaaattg cccagccaat gttgttttcg
     3481 gccaatgtgt cgcgcgaaat cctttgccgt gcatcgccgg gcaccgccac aatcgagatc
     3541 atgagccggg cacggttaag cgattcgcca tggactgtcc acgccaaggc caaggtaata
     3601 ttcaggccgg cgccgtctgt tgctaatgaa acgatcccgg acgtgaccgg agacatcgtc
     3661 gcgagcgccg atctctacag gatcgcgcgc cattccggtc tcgaatttgg tccggcctac
     3721 cgccaagttt ccgcggcagc gcgcactggc caaacgagta tcgtcgtagc gcttttggcg
     3781 gcagatctgt caccgcaata tggcctcgat ccggcgcgcc tcgattcatg ttttcatggt
     3841 ttgatactgc tcttctcaga caaagtttcg agcggtcgcc aagccgccta tctccccgtg
     3901 cgttttggga cgataaggct ggaaaagccc ggcgccgtca tcgcacgtgc gcgtatcgat
     3961 gtccggcgac acgacgcccg cgccatcgtc gccgatttta cgctcgttga cgctgatggt
     4021 actctgatcg ctcagttgca aggcgcgcgc taccaagccg cgcgccgcga ttcgctcgat
     4081 cttaccagcc agtctctcat ccaagttgcg atcctcgctg acgagccaac ggcgattcgt
     4141 cgcaagccgg cgctttccct cgacaccttg gctatggccg tcgttccgga aaccccgaca
     4201 gcgagcgaaa gtcttccccc ggacgtcatt ttaatcgagg gttgggcaac cgctgcagcg
     4261 caccggttca tccgtgcgct tgctgccggc ggatcgatcg atcctgaaag gctgatagct
     4321 aaagggcgcc tcccaccagg gtggcgggtc tggctctaca aattgctcct tgcgctcgag
     4381 aagagcggtc tcgtagcggc tgggccggaa caattcgtat tggcagggaa caatactctt
     4441 cctgacccca cacagatttt gcaaaacatc gctgctgagc acccgcgccg atccgccgaa
     4501 ctgcttctcg ccgctcgatg caatgctatc atggagtctt tgacgcgggg tgacgagcct
     4561 gctccgatag cccaatcagt tttcgaatcg tttgaaacag gttccgtgtc tgtcgccgcc
     4621 gcggcggatc ttcttgcctc attattgcgg cccacgatcg aagcctggcc gcgcgaccgg
     4681 gcgctgcgta tactgcaaat cggccatggt ccgctttccc ggcacgcagt ggcgctcacg
     4741 cagtcgaaag aagcccggct gacgatccac gaccctaatc ccctgaggct tgaacgggcc
     4801 cgcttggctt tcgcaggcaa gccttgcatt agcttcgccg acagtctcga acaggtcccc
     4861 aaaagctctg cggacctcgt catcgcggcc gatgctttgc aaaggttggc gccgggtaag
     4921 gaacagctcg cccggcttgt cgaaaccatg gcacctgggg gccttattgc cgcgatcgag
     4981 cccgcgcctt cgctgttccg cgatctcgtt cttggccttc gtgaggatcc ttcggctgca
     5041 acgaaacata gcgacgatgg cttcctctcg cagccagatt ggctggcaca attttcagcc
     5101 ctgccgctgc gcgggatctc cgtaaaaaat gtcgggactg aagccggtcc ggcacgattc
     5161 attatgggcg agcggccggt gtttgcggaa agccgcacac ttccaaccaa agtgttgatt
     5221 gtcggccagg aagatccgca ccagttcgaa acggcttctg cgctttcgaa tcggctcgaa
     5281 gacgccggag tcgaatgtgc caccagccgc gagcgccctt ttgatcggct ttctctcgcg
     5341 gatttagaga caatcgtttt cctcgctccc gacgcgtcgg ccggcggtgt aacggctgca
     5401 acgattgccc aaaggtgcct ggcactaaaa aattgggcgg cagagcttgg aaaaaacaag
     5461 caccaacttt gggtcgagtg tcccggcgcg ttgcgcacag gtcctggtac gccagacccg
     5521 gttcagacca gcctatggtc ttttgcccgg accttcgcca atgaatacca aacgctcgac
     5581 gtgcgccgcg ttgacacgca ccctagcatg ccggctccgg tcagagcgcg gcggctagcc
     5641 gacgcccttc tttcgggaac ggacgaaacg gaattgctcc tcgactccaa ttccacccgg
     5701 gtcgtgcgca tgcaatcccg caacgcttta aactctcggg gtattgaacg gcctgctcca
     5761 gcgagccgtt tagagaaggg tgacggcaca ggtctcgacc gtatccgctg ggttccgatt
     5821 gatcgtcaat cgccccggcc cgggcaagtg gaaattgctg tgacggcaac cggtcttaac
     5881 ttccgcgacg tcatgtgggg tctctcggtt ctgccggacg aaatgctcga agatgggctc
     5941 gccgggccgg cccttgggct tgaatgcgca ggccggattg tcgcgaccgg agagaatgtc
     6001 gacgatctaa agcccggcga tgcggtcgtt gcagtcgccg ggagggcatt tgccacccat
     6061 gtgaccgtgg acagcaatat ggtcgcaaaa gtgcccgacg gactgccgct cgaggctgcc
     6121 gtcaccatcc ccgttgcttt tctcactgct tattatgccc ttatcaattg cgcccaattg
     6181 aaacctggcc agtgggtgct aatccacggc ggagctggcg gcgttggcct cgctgctctg
     6241 caaatcgccc gctggcgcgg cgcaagggtc atcgcgaccg cgagttcacc tgaaaaacgc
     6301 gatctcgtcg ccgcgcttgg cgccgaacat gtctttgacg cacgcgccaa tgctttcgct
     6361 gacgacgtgc gacgggtgac gggaaagggt gtggatgtcg tcctcaacag cctgtccggc
     6421 gaggcaatgg aactctcagt gggattgctt cagccattcg gccgtttcgt ggagctcggc
     6481 aagcgtgact ttttctccaa cacgcacatt ggtctgcgcc catttcgtaa caaccttacc
     6541 tatttcggag ttgatctcga tcagctcatg ctcgctcagc cggatgttag ccggcaattg
     6601 ttccgggaag tcctagaact tgtcgcacag ggcgaattct ctcctctccc ctatcgcagt
     6661 ttcagggcgc gtgagttcat cgacgcgatg cggcttatgc agcagtccgg ccatgtcggc
     6721 aaaatcgtca tatcgccccc tgaaccaggt gagattggaa ttgcatcaaa cggcgtgttt
     6781 acgcctgcgc gcgaaaagac tcatctcatc accggcggct gtggcggctt tggattggcg
     6841 actgcgtgtt ggcttgccga gcatggggca cgtcacctcg tgctcgccgg gcgtagcggc
     6901 gcttcaagtt ccgaggcgca agaaacagtc gccgatctca gagcaagcgg gataaccgtt
     6961 catgttgagg cgcttgatgt ggccgacaca gacgaggtcg aggcgctctt taagaggatt
     7021 gctcgctcca tgccgccgct ttccggcgtg attcacgccg cggccgtgct cgacgacgcg
     7081 ctgatcgcca atctcgacgc cgagaagctg aagaaagtac tcgcaccaaa ggtgacgggt
     7141 gccgaaaacc tagaccgata tacccacgat ttaccgctcg actatttcgt tctcttttcc
     7201 tccgcaacca cagctatcgg caacccgggt caaggcaact atgtcgcggc caatggcttt
     7261 ctcgaagggt tggcgcggca cagacgcgcg gctggcttgc ccgctctcgc catcgcgtgg
     7321 ggagggatcg aggacgtcgg tttgctcgca cgaaaccgtc tcgtgaagga catgcttgga
     7381 acccgcgcgg gtgtcaaaac catggccgca cgcgacgcat taaatcttat ggctgaagcc
     7441 ctggcgaatc caagcttaga gcccagtgag gcggtactcg tcattagtga aatgaactgg
     7501 tctaccgctc gggcccattt gccgctgctg caatcgccta cgttcagcgg tcttgcccgc
     7561 acgaatgagg cagccgcggc tgaaaaccgc gcaaagctcg acatccgcgc gttggccgca
     7621 acagtctccc ttgaagaagc gcgcaagcaa atcgtcggcg tcatcgtcga agaaatcgct
     7681 catatcctgc ggctgccgca agaaaccgta accaagggca agccgcttgc cgatattggc
     7741 gtcgattcgc ttatggccgt tgaacttgca gccgctttgg aagatcgctt gacgttagag
     7801 gttccgctat ccgcctcggc aagcggattc aacgtaaacg agctggccga ccatattctc
     7861 gggctttgca tcagcccaac gagcgaggac gagtcgatcg cgcaggggct tgcggagcgc
     7921 cacttgggca agggaataga tcccgcagca ctcgaatcgc tgaccgcgct cgttgaggaa
     7981 aggagccgcg atcttacgca aatcttacgc taacttgtcc cgcaaaattg ggaatcggtc
     8041 ctgcgtcctg acagatgaga aaaaagcggg caaataacga ttcgaagaaa aggcctccta
     8101 gtacccagaa tcattgatcc gcgaaacggg gtttgaggcg tttctggtga acctcggatg
     8161 ctgtccactc gaatgggcag gcggtctcgt tgtaggtttc gatgaaagcg tcgatgtggg
     8221 ccttgagttc gtc
//
LOCUS       DATMPU010000367         3323 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_589096,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000367 DATMPU010000000
VERSION     DATMPU010000367.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 3323)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3323)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3323
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_589096"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(115..993)
                     /locus_tag="VNY06_08370"
     CDS             complement(115..993)
                     /locus_tag="VNY06_08370"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009340272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HWX14838.1"
                     /translation="MSVGTAFPEDKTPPGLTVSRRTYITSAAAAAMGYTLAAGPVRAG
                     AIKTDTEGLSVGTAKVKVQDGEMPAYYAKPIAAQKPPIILVAMEVFGLHEHIKDVTRR
                     LGKLGAFAIAPDYYFRLGELTQITEPSRLMPLVNSKSDQELFADLDAAVAWAKEQGGD
                     ADRLGIVGFCRGGRAVWLYATHNPALKAGVAFYGSLMDPPSSPMPKNAFDLAAEVKAP
                     VLGLYGAEDTGITPDQVEAMKERLEAAGKTVKFKIYPGAPHGFFADYRQSYRPEAAKD
                     AWQAMQAWFRRYHVLD"
     gene            complement(1090..2580)
                     /gene="glpK"
                     /locus_tag="VNY06_08375"
     CDS             complement(1090..2580)
                     /gene="glpK"
                     /locus_tag="VNY06_08375"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006022759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HWX14839.1"
                     /translation="MSNNLLAIDQGTTSTRSLLFGPSGRLLATAQCEFAQSYPQPGWV
                     EHDPQEIWRDVLQTARDVIAKSETPVAAIGIANQRETTVVWERAAGTAVHPAIVWQDR
                     RTANLCERLRQDGCEPLIRSRTGLLLDPYFSATKIAWILDHVPGARARAESGKLAFGT
                     IDSFLLWRLTSGKMHATDATNASRTMLFDIHRLCWDEDLLQLFHIPKTLLPEVHDNSF
                     MYGMTAPGLFAQQIPIAGMAGDQHAALIGQACFEPGMGKATYGTGCFVLLNTGQDALS
                     PAKGILTTLGYHAAGNTAYAVEGSVFAAGAVIKWLRDQLELFKDVSQTAAMAAEIDNT
                     EGVYLVPAFTGLGAPYWDPAARGLICGLTLDSKPAHLVRAGLESGAYQTLDLLRAMPG
                     GGAAPSALRVDGGMVTNDWFCQFLAGILGAPVERPALLESTAQGAAFLAGLTLGIFPD
                     LSAISAQWKCGARFEPRMAPAERERRIAGWHDAVRRTLASNKQPVK"
     gene            complement(2759..>3323)
                     /locus_tag="VNY06_08380"
     CDS             complement(2759..>3323)
                     /locus_tag="VNY06_08380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HWX14840.1"
                     /translation="GLAESTIVIISAKHGQSAIDVTKRKTYSDSKVIAGPIGSNFSFD
                     IGDDGVLIWLKGNTGTKTADAVAALNAYGDTGIGEWLSGPFLPLSYQNPAHDSRTPDI
                     IGIAKIGIYTGGTKLAEHGGFNEDDTHVALLLAHPDLEERSINAAVATTQIAPTILKL
                     LGLNPGELEAAQLEGTPILPAIWSEGK"
BASE COUNT          685 a          976 c          960 g          702 t
ORIGIN      
        1 tgcccccgtc ctttagaaat atggcagcaa cagaccgttt gccagcccgc cgggccgtgc
       61 cggcgaactg gctaggctcg cgggccgggc aaagcatcag accaacgcaa caggtcagtc
      121 caatacgtga tatcgtctga accaggcttg catggcctgc caggcgtcct tcgcggcttc
      181 cggccgatag ctctgccggt aatcggcaaa gaaaccatgc ggcgcgcccg gataaatttt
      241 gaacttcacc gtttttcccg ctgcctcgag ccgttccttc atggcctcga cctgatccgg
      301 cgtgatgcct gtgtcctccg caccataaag gccaagaacc ggcgctttga cctcagctgc
      361 aagatcaaaa gcgttcttcg gcatgggcga actaggggga tccatgaggc tgccgtaaaa
      421 ggcaacgccc gccttgagag cggggttatg cgtagcatat agccaaaccg cacgtccgcc
      481 gcggcaaaaa ccaacaatac cgagcctgtc cgcgtccccg ccttgctcct tggcccaggc
      541 gacggcggcg tcaagatcgg cgaatagctc ctgatcggac ttgctattga ccaaaggcat
      601 gagccgcgat ggctcggtaa tttgggtgag ttctccgagc cggaaataat agtctggcgc
      661 gatagcaaag gcacctaact tgccaaggcg ccgcgtgaca tccttgatgt gctcatgcaa
      721 gccaaaaacc tccatggcaa cgaggatgat gggcggcttt tgagctgcaa tgggtttcgc
      781 ataataggct ggcatttcgc catcctgcac tttgaccttt gcggtgccaa cggaaaggcc
      841 ttccgtatcc gtcttgatcg ctcccgcgcg aacagggccc gcggcaagcg tgtatcccat
      901 cgcggcagcg gcggcacttg tgatataggt acggcgcgag accgtgaggc cagggggagt
      961 cttgtcctca ggaaaggcag ttcccacgct catgccatca ctccttttgc tttccagcgg
     1021 cgtctatttg ctgggagaga acttctcaaa atcctacaag atccacgatt ttggtcattt
     1081 cctgggccgt catttaactg gctgtttgtt cgaggcgagc gtgcgacgga ccgcgtcatg
     1141 ccagcccgca atccgtcgtt cccgctcggc tggagccatg cgcggttcaa atctcgcgcc
     1201 gcatttccat tgagccgaga ttgctgacaa atcggggaat attccaagcg tgagcccggc
     1261 caaaaatgca gcaccctgag cggtcgactc gagcagggca gggcgttcga ccggcgcgcc
     1321 gagaatgcca gcgagaaact gacagaacca atcgttggtc accattcctc catcgacgcg
     1381 cagcgcgctc ggagccgcgc cgccaccggg catcgctcgt aagagatcaa gcgtctgata
     1441 ggctcccgat tccaatccgg cgcggacgag atgggcaggc tttgagtcta gggtgaggcc
     1501 gcaaatcagc cctctggcag cagggtccca atatggcgcg ccgaggccgg taaaggcggg
     1561 cactagatag actccctcgg tgttgtcgat ctccgccgcc attgcggctg tctgggacac
     1621 atctttgaag agttcgagtt gatcacgcaa ccatttgatc actgcacccg ccgcgaagac
     1681 cgatccttcg accgcatagg cagtgtttcc ggcagcatgg taaccaaggg ttgtaagaat
     1741 gccctttgcc ggagacaaag catcttggcc ggtgttgagc aagacgaaac agcccgtacc
     1801 ataggttgcc tttcccattc ccggttcaaa gcatgcctgg ccaattagtg ccgcatgctg
     1861 gtcgcccgcc atgcctgcaa tcggaatttg ctgggcaaaa agtccaggcg ctgtcatccc
     1921 atacataaag ctgttgtcat gaacctcggg caggagcgtt ttgggtatat ggaacaattg
     1981 caaaagatcc tcgtcccagc acagccggtg gatatcgaac agcatggtac gcgaggcgtt
     2041 tgttgcatct gttgcatgca tcttgccgct tgtcaggcgc cagagcaaaa agctgtcaat
     2101 ggtgccgaag gcaagtttgc cgctctccgc tcgtgccctt gctcccggaa cgtgatcgag
     2161 aatccaggca atcttggttg ccgaaaaata aggatctaga agcagccctg tgcgagaacg
     2221 gatgaggggc tcgcagccat cctggcgcaa ccgttcgcac aaattagcgg ttcgccggtc
     2281 ctgccagacg atggcgggat ggaccgccgt gcccgctgcg cgctcccaaa cgacggttgt
     2341 ttctcgctga ttcgcgatcc cgatcgccgc gacaggtgtc tctgattttg cgatgacatc
     2401 gcgcgcagtc tgcagaacat cgcgccagat ctcttgggga tcatgctcaa cccatcccgg
     2461 ttgcggatag gattgcgcaa actcgcattg cgccgtggcg agaagccgtc ctgaagggcc
     2521 gaagaggagg ctcctcgtcg aggttgtgcc ctggtcaatg gcaaggagat tgttggacaa
     2581 gcggcggcct cccgagcgtc acgagaggcg gatcgctggc gcctcctgcg ttttcacgag
     2641 tttagcgcac ggacccccgc attgcgaagg ttcaattcca ttgaacgaga cttgggacag
     2701 tgcgcggcag atgcccatct taaaggcgcc gggagcgccc ggcggccccg aagacgcttt
     2761 actttccttc actccatatg gcgggtagga tcggcgtgcc ttcaagttgc gcggcttcga
     2821 gttccccggg gttgagccca agtaatttga ggatcgttgg cgcaatctgc gtcgtcgcca
     2881 ctgcagcgtt gatcgacctt tcttcgaggt ctggatgcgc caacagaagc gcgacatgcg
     2941 tatcgtcttc gttgaagccg ccatgctccg cgagcttcgt tccacctgtg taaataccga
     3001 ttttggcaat tcctataatg tccggagtgc gcgaatcgtg cgccgggttc tgataggaca
     3061 acggaagaaa gggaccggac agccactcgc ctatacctgt atccccataa gcattaagtg
     3121 cagcgactgc gtccgccgtt ttcgttccgg tattgccctt gagccagatc aggacgccat
     3181 catcgccgat gtcaaaagag aaatttgatc cgatcggccc ggcgatcacc ttgctgtcgc
     3241 tgtaagtctt ccgtttcgtg acgtctattg ccgactggcc gtgcttggcg ctgatgatga
     3301 caatcgtgct ttcggcgagg cca
//
LOCUS       DATMPU010000368         2973 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_925006,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000368 DATMPU010000000
VERSION     DATMPU010000368.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 2973)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2973)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2973
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_925006"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1871)
                     /locus_tag="VNY06_08385"
     CDS             complement(<1..1871)
                     /locus_tag="VNY06_08385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012589549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HWX14841.1"
                     /translation="MLQSLRASAQGRFGRAIMAVLMALIILSFAIWGIGDIFRGFGAN
                     DLARVGKIEIGADTFRNAYQTELQRQQRLERRNITSEEAHQNGMDRQVLSRLVADAAL
                     DDQARTLGLAISDEDIKKAIMNDENFKGMTGTFDRQILAAYLREEGFTEKTYIQNQRA
                     VYLRRQILDSLTAGVQLPKALLEAIFRFQMEGRGVDYIVLPFSSVGQLPAPTYKELEQ
                     FFENNRGLYAIPEYRSLVILEVTPQSIAKPESVSDADARARYEEVMYGRFGAPEKREI
                     EQILYASEAEASEVRHKLSTGKTFEDLLKEKNLTSKDASLGMVARNGLVDKAVAEAAF
                     SLKKDEVSAPIQAQFGTVILRVRKIIPSTVKPYDEVAATIKGEIALNRAQREIAKLHD
                     AIEDQRTYGKSLTEAAQGVGLKPRAIAAIDPAGNDPQGAPVKDLVDGPALLKAAFASD
                     IGVDNDTLRLANGGYQWFEVAKIDKARDKSFEEAKPDVEKAWREEQVGKRLSAKTAEL
                     VRKLDAGESMASIAAAEGKLTVKHESNVRRGLSSGLAQNFVNEIFNVNVHRAGTTPNE
                     DGGAYLFVVTDSSVPPFDPQSPELTNVKTDAKNGYIEDLIAQYITKLEGEIGVKLNA"
     gene            2034..2792
                     /gene="tpiA"
                     /locus_tag="VNY06_08390"
     CDS             2034..2792
                     /gene="tpiA"
                     /locus_tag="VNY06_08390"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020175713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HWX14842.1"
                     /translation="MANDVPPLVAGNWKMNGLRNSLGEVALMRDAVAAGGAGTAEVLI
                     CPPATLLMAVSGICEGFPLKVGGQDCHAEPSGSFTGDISAEMLKDAGASYVILGHSER
                     RAGHRENNETVRLKALAARRAGLTAIICVGETIGEREKGQALAIVGTQLSGSIPKNSL
                     LGGIVVAYEPVWAIGTGLTPEHKEISEMHRFIREKVDQNLPGQGANLRILYGGSVKPE
                     NAAQLLSAADVDGALVGGASLSSAAFLEIAKFYR"
BASE COUNT          600 a          811 c          851 g          711 t
ORIGIN      
        1 ttggcgttaa gtttcacgcc aatttcgcct tcaagcttgg tgatatactg ggcaatcaaa
       61 tcttcgatat atccgttctt cgcatcggtt tttacgttgg tgagctcggg ggactgcggg
      121 tcaaatggcg ggacggagga gtctgtcacc acaaacaaat atgcgccgcc gtcctcgttg
      181 ggcgtggtgc ccgcgcggtg cacattcacg ttaaatattt cgttgacgaa attttgcgct
      241 agtccacttg acaggcctcg ccggacattg ctttcatgct taacggtgag cttgccttcc
      301 gctgccgcga tcgacgccat gctttctccg gcgtcgagct tcctcacaag ttcagccgtc
      361 ttcgccgaga ggcgcttgcc cacctgctcc tcgcgccaag ccttttcgac gtcgggtttt
      421 gcttcctcaa aagatttgtc acgggccttg tcgattttgg caacctcaaa ccattgatat
      481 ccgccatttg ccagccgcaa agtgtcattg tcaacgccaa tatcggaggc aaaggctgct
      541 tttaacagag caggaccgtc gaccaaatcc ttgacgggcg caccctgcgg gtcattgcca
      601 gccggatcaa tcgcagcgat cgcccgcggt ttcaggccga caccttgtgc ggcttcggtt
      661 agcgattttc cgtatgtgcg ctggtcctca atggcatcgt gcaatttggc aatttcgcgt
      721 tgcgcacggt tcagcgcaat ctcccctttg atcgtcgcgg caacctcatc ataaggcttg
      781 actgtggagg ggatgatttt gcggacacgc agaataaccg tcccaaattg ggcttggatc
      841 ggcgcgctca cctcatcttt cttaagtgaa aaggcggctt ctgcgaccgc tttatcaacg
      901 agcccattgc gcgcgaccat gccgagactc gcatccttgg aagtgaggtt tttctccttt
      961 aagagatctt cgaatgtttt cccggtactg agtttatggc gtacctcact cgcttccgct
     1021 tcgctggcgt agagtatctg ctcaatctcg cgtttttcgg gcgccccaaa tcgcccgtac
     1081 atgacttctt cgtagcgggc ccgtgcgtca gcgtcagaga cgctctcagg tttggcgatc
     1141 gattgcggtg tcacctcaag aatcacgagg ctgcgatatt ccgggatggc gtaaaggccc
     1201 cggttgtttt cgaagaattg ttcgagctcc ttgtatgtgg gcgcgggcaa ttggccgacg
     1261 ctcgaaaagg gtagcacgat atagtcgacg ccacggccct ccatctgaaa gcggaagatt
     1321 gcctctagca atgcctttgg cagttgcacg ccggcggtta aggaatcgag gatttgacgg
     1381 cgcagataaa cagcgcgctg attttgtata taggtctttt cggtaaatcc ttcctcgcgg
     1441 agatatgccg cgaggatttg gcggtcgaag gtgcccgtca tccccttaaa attttcatcg
     1501 ttcattatgg cttttttgat atcctcatca ctgatcgcca gcccgagcgt ccgggcttgg
     1561 tcatcgaggg cggcatcggc gacgaggcgc gagaggacct gacggtccat tccattttga
     1621 tgcgcctctt cgctggtgat attgcggcgc tcgagacgct gttggcgttg cagctccgtc
     1681 tgataggcat tgcggaaagt gtcggcaccg atttcaattt tgccgacccg ggcaagatca
     1741 ttggccccga accctcggaa aatatcgcca atgccccaga tcgcgaaact caggataatc
     1801 agtgccatga ggaccgccat gatagcgcgg ccaaaccggc cttgtgccga ggcgcgcaat
     1861 gattggagca tgtgatgaag tccctattga acgtgatctg gggaaggggg agctagccga
     1921 tccttacgcg gcttttgcgc cggcccgggt ttcacaaagc gtgcgcgaac tataaaaaga
     1981 tgcgacttca catgcaacgc ggctttgtcc cgaagttatg ggaatatttc tatatggcca
     2041 atgacgtacc ccctctcgtc gcgggcaact ggaaaatgaa tggcttgcgc aacagcctcg
     2101 gcgaagtcgc cttgatgcgg gatgccgtgg ccgccggcgg cgcgggaaca gcggaggtgc
     2161 tgatttgccc gccggcaacg cttctcatgg ccgtgtccgg gatatgtgaa ggcttcccgc
     2221 ttaaggtcgg cggtcaggac tgccatgccg agccgagcgg atcctttacc ggtgatattt
     2281 ccgccgaaat gctcaaggat gctggagcaa gctatgttat cctcgggcac tccgagcgcc
     2341 gggccggaca tcgcgaaaac aatgagaccg tccgcctgaa ggctctggcg gcgcggcgtg
     2401 cagggctgac agcgattatc tgcgttggcg aaacgattgg cgagcgggaa aaaggtcagg
     2461 ccttggcgat cgttggcacg caactctccg gctctattcc aaaaaattcg ctgctgggcg
     2521 gcattgttgt ggcctacgaa ccggtttggg caatcggaac gggcctaacc cctgagcaca
     2581 aagagatcag cgaaatgcac cgctttatcc gcgaaaaagt agaccagaat ttgcctggac
     2641 agggcgcgaa tttgcgcatt ctttacggcg gctcggtgaa acccgaaaat gccgcgcaat
     2701 tgctttcggc cgcggatgtg gatggcgcgc tcgttggggg tgcgagcctc agttcggcgg
     2761 ctttcctgga aattgcaaag ttttaccggt aacgcaacgc cgcaaagcag tgccgatcgg
     2821 cgcggttttt ggaaaaacgg cgtcggatgc ccagcttccc gagcaagatg acttttcttc
     2881 aaatcgtcat cttgctctag tacaccgttg ggcgacaaaa gagcctaagg atagggctta
     2941 ttagtttgcc tggatggtac ccacggcgtc ctt
//
LOCUS       DATMPU010000369         7713 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_2446506,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000369 DATMPU010000000
VERSION     DATMPU010000369.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 7713)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7713)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7713
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2446506"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            73..645
                     /locus_tag="VNY06_08395"
     CDS             73..645
                     /locus_tag="VNY06_08395"
                     /EC_number="2.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012385967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0018104 - peptidoglycan-protein
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HWX14843.1"
                     /translation="MPRPYSRRPYAYGAPDEEIYGPTGHSQSYGGDNLEVGVNFNQRT
                     AVVVPNPTSEPPGTIIINTNARQLYLVEQGGTAIRYGIGVGRQGFTWKGVAHVGRKSE
                     WPRWIPPREMLKRRPDLPEEMDGGLENPLGARALYLFQGNKDTLFRIHGTNEPDSIGK
                     AVSSGCIRMMNADVIDLFGRVRVGTKVVVL"
     gene            complement(950..2368)
                     /locus_tag="VNY06_08400"
     CDS             complement(950..2368)
                     /locus_tag="VNY06_08400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008139023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit beta"
                     /protein_id="HWX14844.1"
                     /translation="MGKVTGFLEIDRRDRRYVPASDRIRSYKEFLIPLSEEATKDQAA
                     RCMDCGIPFCHSGCPVNNQIPDWNDLVYRSDWEEASRNLHSTNNFPEFTGRLCPAPCE
                     AACTLNIDDIPVTIKTIECAIADRAIAKGWIKPEPADAKTGKTVAVVGSGPAGLACAQ
                     QLARAGHDVHVYEKNAKPGGLLRYGIPDFKMEKHLIDRRIAQMQAEGVHFHNSVNVGV
                     DLDPGDLIARFDALILAGGAEKPRELKIPGCELSGVYLAMDFLPQQNRRIAKEPIHSN
                     VPILASGCHVVVIGGGDTGSDCIGTAIRQGALSVTQLEIMPQPPDKENKLLTWPDWPL
                     KLRTSSSQEEGAERDFSVLTKWFHGVDGAVKSVVCARLDRNFKEIPNSTFEFRADLVL
                     LAMGFVSPVQEGLLERLGIAFDPRGNVKADEKNYKTSVPKAFACGDMRRGQSLIVWAI
                     REGRQAARAVDEFLMGSSQLPR"
     gene            complement(2457..7133)
                     /gene="gltB"
                     /locus_tag="VNY06_08405"
     CDS             complement(2457..7133)
                     /gene="gltB"
                     /locus_tag="VNY06_08405"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012385942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HWX14845.1"
                     /translation="MTVPIFSSPIFRDPGLPAAQGLYSPANEHDSCGVGFVVNMHNLK
                     SHELITMGIEILHNLNHRGAVGADPEAGDGCGMLVQIPHRFFAEKAKALCFDLPAPGN
                     YAVGALFLPRDAEGRLIVEAIVEKIVAEEGQTILGWRDTPVDSSCLGETVKESEPLGR
                     QIFIGRGPGIEDQTVFERRLYLLRKRISNAVRDLCDPRTNGFYPVSMSSRTIVYKGLL
                     LATKLSCYYKDLADPLFESALCLVHQRFSTNTFPTWSLSHPYRLVAHNGEINTLRGNV
                     NWMAARQASVASLLFGDDINKLWPISYEGQSDTACFDNALEFLTQGGYSLAHAMMMLI
                     PEAWAGNPLMDSARRIFYEYHAAMMEPWDGPAAVAFTDGRQIGATLDRNGLRPARYLV
                     TSDGLVVLASEAGVLPIPEEKIITKWRLQPGKMLLVDLEQGRIISDDEIKTTLAASHP
                     YAEWLRRTQIVLEDMDLAVHPRAPRSDVSLLDRQQAFGYTQEDISLLLPPMSVTGQEA
                     VGSMGTDTPISALSTQSKLLYTYFKQDFAQVTNPPIDPIREELVMSLVSFIGPRPNIL
                     DLEGSATKKRLEVRQPILTNGDLEKIRSISHFEEAFDTKTLDFTYPSESGAEGMSDAL
                     AKLCSRAEQAVNSGFNIIILSDRMTGPDRVPIPALLATAAVHHHLIRKGLRTSVGLVV
                     ETGEAREVHHFALLAGYGAEAVNPYLAFDTLAALAKDFPDEIDGHEACKRYIKAIDKG
                     LLKVMSKMGISTYQSYCGAQIFEAVGLSEDFVLRYFTGTATGIGGAGLSEIAQETVRR
                     HREAFGDAPIYRNALDIGGDYAFRIRGEAHSWSPQSVSLLQHAVRGKDEATYHAYAAL
                     LNEETERPLTIRSLFRIKAAEEGGRQPVPIEEVEPASSIVRRFSTGAMSFGSISREAH
                     TTLAIAMNRIGGKSNTGEGGEESDRYKPLSNGDSMRSAIKQVASGRFGATTEYLVNSD
                     VMQIKMAQGAKPGEGGQLPGHKVDAVIAKVRHSTQGVGLISPPPHHDIYSIEDLAQLI
                     FDLKNVNPKAGVSVKLVSEIGVGTVAAGVSKARADHVTISGFEGGTGASPLTSIKHAG
                     SPWEIGLAETQQTLVLNRLRSRIAVQVDGGLRTGRDVVIGALLGADEFGFATAPLIAA
                     GCIMMRKCHLNTCPVGIATQDPVLRKRFTGQPEHVINFFFFVAEEVRQLMARTGFRTF
                     NEMIGQMQMLDKQQAVAHWKAKGLDFSRLFHKPAAAANDPIFHSTEQDHGLEKVLDRK
                     LIAAAKASIETGARATIETAINNTDRVTGAMLSGEIACRHGHAGLKDDTIRIKAKGTA
                     GQSFGAWLAAGVTLELEGEANDYVGKGLSGGRIIVYPPSSSRITPEKSIIIGNTALYG
                     AIAGECYFRGVAGERFAVRNSGAIAVVEGLGDHGCEYMTGGIVVVIGDTGRNFAAGMS
                     GGIAYVLDEDGKFAQRCNLAMVDLEPVVEEEDLMQKNYHHGGDLDFHGRVDVMNDMTR
                     FDAERLHQLISNHARHTNSARARHILDHWDEYKPRFRKVMPVEYRRALEELIAKGVEP
                     KLAAAE"
     gene            7520..>7713
                     /locus_tag="VNY06_08410"
     CDS             7520..>7713
                     /locus_tag="VNY06_08410"
                     /inference="COORDINATES: protein motif:HMM:NF013384.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-eliminating lyase-related protein"
                     /protein_id="HWX14846.1"
                     /translation="MNFASDNTVRTSQAILKAVVAANEGESPAYGKDPWSAKAAGLVR
                     EAFDRDCTCFLVNTGTAANAL"
BASE COUNT         1654 a         2227 c         2190 g         1642 t
ORIGIN      
        1 cccggttatc cgtcctatcc ggccaatcca aattatcccg agtcttatgt cgaccccgtc
       61 gctcctccgg ccgtgccacg tccttactct aggcgccctt acgcatatgg agcacctgac
      121 gaagagattt atgggccaac ggggcacagc caatcttatg gcggcgacaa cctcgaggta
      181 ggcgtcaact tcaatcagcg gaccgcagtt gtcgtgccaa atccgaccag tgaaccgccg
      241 ggcacgatta tcattaatac aaatgcgcgt cagctatatc ttgtggagca aggaggcact
      301 gcaattcgtt acggtattgg tgtcggccgg caagggttca cgtggaaagg cgttgcgcat
      361 gtcgggcgga aatctgaatg gccccgttgg ataccgccaa gggaaatgct gaagcgccgc
      421 cccgacctgc cggaggaaat ggacggcggg ctcgaaaatc ccctcggcgc ccgtgccctt
      481 tatctgttcc aaggcaacaa agatacgctg tttcgcattc acggcaccaa tgagccggac
      541 tcaatcggca aggctgtctc gtccggctgc atcaggatga tgaacgccga tgtcattgat
      601 ctttttggac gggttcgtgt cggaacgaaa gtcgtcgttt tgtaaaccag cggcgcgtcg
      661 tttagggcca gagtccggct tgtagcttgg catcaaacgc ttggttccag tcgggctccg
      721 cgggctctgc ctgttccatg ttgaccgcat cgacgccgcg gtcaataaga ccgagtgggg
      781 attcgactga ggtccgcgtt atttagcaat gggcaaaacc ggcccacccc ggcggcacgg
      841 aacattccgt aaaaagcagg accgagccaa ctgaaatgac aacacgttgg aacataacgg
      901 cgtaaagctg ggcttgttgc cgagaaggaa tgggagtttt ggcggcactt taccgcggta
      961 actggctcga tcccatcaag aattcatcga cggcgcgggc ggcctgacgg ccttccctta
     1021 tcgcccagac aataagcgat tgaccacggc gcatatcacc gcaggcgaag gcttttggta
     1081 ccgaggtctt gtagttcttc tcatcggcct tgacattgcc acgtggatcg aacgctatgc
     1141 cgagacgttc caaaagcccc tcctgcacgg gggaaacgaa gcccatcgca agaagcacga
     1201 ggtcagccct gaactcaaag gtgctgtttg ggatttcctt aaaattccta tcgagcctcg
     1261 cgcacacgac gctttttacg gcgccgtcca cgccgtggaa ccatttcgta agcaccgaaa
     1321 aatccctttc tgcgccttcc tcctgcgagg aggatgtgcg cagtttcaag ggccaatctg
     1381 gccaagtgag aagcttgttt tccttgtccg gcggctgcgg catgatttcg agctgggtta
     1441 cggaaagcgc accttggcgg atcgcagtgc cgatacagtc cgacccggta tcaccaccgc
     1501 cgatcacaac gacatggcaa cccgacgcta ggattggcac attgctgtga atcggctctt
     1561 tggcaatgcg ccggttttgt tgcggcagaa agtccatcgc caaataaacg ccagagagct
     1621 cgcaccctgg aattttaagt tcgcgcggct tctcggcgcc gccggcaaga atcaacgcgt
     1681 caaagcgtgc gatcaagtcg cctggatcga ggtcgacgcc gacgttcacg ctgttgtgga
     1741 aatgcacgcc ttcggcctgc atttgcgcga tccgccggtc gatcaggtgc ttttccatct
     1801 tgaagtcggg gatgccataa cgcagcaaac cacctggctt ggcatttttc tcatagacat
     1861 ggacgtcgtg cccagcacgc gcgagctgtt gggcacatgc aagccccgct ggccccgagc
     1921 cgacgactgc gaccgttttg cccgtcttcg catcggcagg ttcgggtttt atccagcctt
     1981 ttgcaatggc gcggtctgcg attgcgcatt cgatggtctt gatcgtcacc ggaatatcgt
     2041 cgatgttcag cgtgcaagct gcttcgcaag gtgcaggaca aaggcggcca gtgaactccg
     2101 gaaaattatt ggttgaatgg aggttgcgag atgcctcttc ccaatctgag cgatagacga
     2161 gatcattcca atcgggaatc tgattattga ctgggcagcc gctgtggcaa aacggaatgc
     2221 cgcaatccat gcaacgcgct gcttgatcct ttgtcgcttc ctccgaaagg ggaattaaga
     2281 attccttata gctgcgaatc cgatcagacg ccggcacgta gcgccggtcc cgccggtcga
     2341 tttcaaggaa tcccgttacc ttgcccatgc gtcgcaaagt tccccaatac ctctaaaccg
     2401 ctttccgcgc tccccaatcc caactggggc caaaggttcg ccgacgcttc ttccgtttat
     2461 tccgccgccg cgagctttgg ctctaccccc ttggcgatga gctcctcaag tgcgcggcgg
     2521 tactcgaccg gcatcacctt gcgaaatctg ggcttgtact catcccaatg gtcgagaatg
     2581 tgacgagccc gcgcagaatt cgtatggcgc gcgtgattag aaatgagctg atgcagccgc
     2641 tcggcatcaa aacgagtcat gtcgttcatg acatcgaccc gcccatggaa atcgagatca
     2701 ccgccgtggt gatagttctt ttgcatcaaa tcttcttctt ccacgacagg ttcgagatca
     2761 accatagcga gattgcagcg ttgcgcaaac ttgccgtctt catcgaggac ataggcgata
     2821 ccgcccgaca tgcctgcggc gaagttgcgc cccgtatcgc cgataacgac aacaattccc
     2881 ccggtcatat attcgcagcc gtgatccccg agcccttcga cgaccgcaat cgcgccggag
     2941 ttgcgcacgg caaagcgttc ccccgcaacg ccgcgaaaat agcattcccc ggcgattgcg
     3001 ccatagagcg cagtattgcc aatgatgatc gatttctctg gagttatccg cgaagatgac
     3061 gggggataaa cgatgatacg cccgccagag agccctttgc ccacatagtc attcgcctcg
     3121 ccttcgagct cgagcgtgac accggctgca agccaggccc caaaactttg cccagcggtt
     3181 cctttcgctt tgatgcggat cgtgtcgtct ttaagcccgg catggccatg acggcaggca
     3241 atttcccccg acagcatggc gccggtgacg cgatctgtgt tgttgatggc agtttcaatg
     3301 gtcgcccggg cgccggtctc gatcgatgcc ttggccgctg cgatcaattt gcggtccagc
     3361 accttctcaa ggccatgatc ctgctccgtc gaatggaata tcggatcgtt tgccgccgct
     3421 gcgggtttgt gaaagagccg cgagaagtca agccccttcg ccttccaatg tgcaacggcc
     3481 tgctgcttgt cgagcatctg catctgaccg atcatctcat tgaaagtgcg aaagcccgtc
     3541 ctcgccatca attgacgcac ttcctcggcg acgaagaaaa agaaattgat gacatgctcc
     3601 ggttgacctg tgaatctttt acgtaggact gggtcttgcg tcgcgatgcc gaccggacag
     3661 gtgttcaaat ggcatttgcg catcatgatg caaccggccg cgatcaacgg cgctgtggcg
     3721 aagccgaatt cgtcggcacc gagcaatgcg ccgatcacga catctcggcc ggtgcgtaat
     3781 ccgccatcga cctgtaccgc gatccgcgag cgcagccggt tcaacaccaa ggtctgctgc
     3841 gtctcggcga gaccgatttc ccaggggcta cccgcgtgct taatcgaagt gaggggcgag
     3901 gctcctgtcc ctccctcgaa accggaaatg gtgacgtgat cggcgcgagc cttggagacg
     3961 ccagcggcaa cggtcccgac accaatctcg gaaaccagtt tgaccgagac accggctttt
     4021 gggttgacat tttttaagtc gaagatgagc tgcgcaagat cctcgatcga atatatgtcg
     4081 tggtgcggtg gcggagatat caggccaaca ccttgcgtcg aatgccggac cttggcgatg
     4141 actgcgtcga ccttgtggcc aggcaattgg ccgccttcgc caggcttcgc cccttgtgcc
     4201 attttgatct gcattacatc ggaattgaca aggtattcgg tggtggcgcc aaaccgccca
     4261 gaagcgacct gcttgatcgc tgaacgcatc gaatcgccgt tggagagcgg cttatagcgg
     4321 tcggactcct cgccgccttc gccagtattg gatttgccgc cgatccggtt catcgcgatg
     4381 gccagcgtcg tgtgcgcttc ccgcgaaatc gagccaaagg acatcgcccc ggtcgaaaaa
     4441 cgccgcacga tagaacttgc cggctcgacc tcttctatcg gcacgggctg gcggccgcct
     4501 tcttctgccg ccttaatccg gaacaggctg cggatagtca atggccgttc ggtttcctcg
     4561 ttcaaaagtg cggcataggc gtggtaggta gcctcgtcct tgccacgcac ggcatgctgc
     4621 aacaatgaca ccgattgcgg cgaccaggaa tgcgcctccc cgcgaatgcg gaacgcataa
     4681 tcgccgccga tatcgagtgc attgcgatag atcggagcat ctccgaatgc ttccctgtgg
     4741 cgccgcacgg tctcctgcgc gatctcgctt agcccggcgc caccgatacc tgttgcagta
     4801 ccggtgaaat agcgcaagac aaaatcttct gaaaggccga ccgcctcgaa gatctgcgcg
     4861 ccgcaatagg attgatatgt cgaaatgccc attttcgaca tgacctttaa gaggcccttg
     4921 tcaattgctt tgatgtagcg cttgcacgcc tcatgtccat cgatctcatc gggaaagtcc
     4981 ttggccagcg cggccagcgt gtcgaatgca agataggggt tcactgcttc ggccccatag
     5041 cccgcaagca gagcgaagtg atgcacttcc cgggcttcac ctgtctcgac gacgagaccc
     5101 accgaagtgc gcagaccctt gcgaatgaga tgatggtgca ccgccgcggt tgcgagaagc
     5161 gctgggatcg gtacgcggtc tgggccagtc atgcggtcgg acaagatgat gatgttgaag
     5221 ccgctattga ccgcttgttc cgcccggctg caaagttttg ccaaagcgtc ggacatgcct
     5281 tcggcgccac tttccgaggg ataggtaaaa tcgagtgttt tggtgtcgaa agcctcctcg
     5341 aaatggctga tcgagcgaat tttctcaagg tcgccattgg tcaagatggg ctgacggact
     5401 tcaaggcgct tctttgttgc gctgccttca aggtcaagga tgttgggacg cggtccgatg
     5461 aaggacacga ggctcatgac caattcctcg cggatcggat cgatcggagg attggtcacc
     5521 tgcgcgaaat cctgcttgaa ataggtataa aggagctttg attgtgtcga tagcgccgag
     5581 attggcgtat cggtgcccat cgagcccaca gcttcctgtc cggtgaccga catcggcggc
     5641 aggagcagtg aaatatcctc ttgcgtgtag ccaaaagctt gctggcgatc gagcagcgac
     5701 acgtcgctgc gtggcgcacg gggatgaact gcaaggtcca tgtcctcgag cacgatctgg
     5761 gtacgcctta gccattccgc ataagggtgc gacgccgcaa gcgtggtctt gatttcgtca
     5821 tccgagatga tccggccttg ctccagatca acgagcagca tcttgcccgg ttgcagccgc
     5881 catttggtaa tgatcttctc ctcggggatc ggcagaacgc cggcctccga agcgagcacg
     5941 acgagcccgt cgctggtaac gaggtagcgc gccgggcgca gaccattgcg gtcaagcgtc
     6001 gcgccgatct gacgcccgtc ggtgaatgcg acggcggccg ggccgtccca tggctccatc
     6061 atcgcggcat gatattcgta aaaaatgcgc cgcgccgaat ccataagagg attgcccgcc
     6121 caagcctccg gaatgagcat catcatcgca tgggcgagcg aatagccgcc ttgggtgagg
     6181 aactccagcg cattgtcaaa acatgcggtg tccgattgac cttcatagga gatcggccaa
     6241 agtttgttta tgtcgtcgcc gaaaagcaaa gaagcgaccg aggcttgccg cgccgccatc
     6301 caattgacat ttccgcgcaa cgtattgatt tcgccattgt gtgcgaccag ccgatagggg
     6361 tgcgatagcg accaggtggg aaaggtgttc gtggaaaacc gctgatgcac aaggcacagc
     6421 gcggattcga acaagggatc ggcgaggtcc ttatagtagc agcttagctt cgttgcgagc
     6481 aggaggcctt tatagacaat cgtgcgtgac gacattgata ccggataaaa tccgttggtc
     6541 cgcgggtcgc ataggtcgcg aaccgcattc gaaatcctct tgcgcaaaag ataaagccgc
     6601 cgctcgaaca ccgtctgatc ctcgatgcca ggaccccggc cgatgaaaat ctggcggcct
     6661 aacggttcgg actccttgac ggtctcccca aggcaagagg aatcgacagg cgtatcgcgc
     6721 cagccgagga tggtctgtcc ttcttctgcg actatttttt caacaatagc ttcaacgata
     6781 aggcggccct cggcgtcgcg cggaaggaac aatgcgccaa cagcataatt gccgggcgcc
     6841 gggaggtcga aacaaagagc ctttgccttt tcggcaaaaa aacggtgcgg aatttggacc
     6901 agcatgccgc agccatcgcc agcctcggga tcggctccga ctgcgccacg atggttgaga
     6961 ttgtgcaata tctcaatgcc catggtaatg agttcatgtg acttgagatt gtgcatgttc
     7021 acgacgaagc cgactccaca ggagtcatgt tcgttggcgg gcgaataaag gccttgcgct
     7081 gccggcagac caggatcccg gaaaattggg ctggaaaaga tcggaaccgt catctcttaa
     7141 ccctttgcaa actttccttc tttgccgcag cgagggctcc gcgcgcgagc gaagcgcgct
     7201 tgacgagccc aacacttctg gcaagaactc aatggcgtgg caagaagcgc gccaaacgcg
     7261 ccagcgcgtg tctcagctgg gaatttgcgc gaactctgca cgccaggcag acgctggcaa
     7321 gagtgctcca gtccgaatgg tcagcagcac tgtcctaagg attaaagatt gcagatttcg
     7381 ggggcgttca caagtgcgga aaatagtctt tccgaaacca cgcctgctat acagttgagt
     7441 cttggcgccc cgatccgcgt ttattcgagg ccgccggcag gccggacttt gcttcgccca
     7501 ttccttggca ctacccgcga tgaattttgc aagcgacaat acggttagaa caagccaagc
     7561 cattctcaag gccgtcgtgg cagcaaatga aggcgagtct ccggcctacg ggaaggatcc
     7621 ttggagcgca aaggccgcag ggctggtgcg cgaagcattt gaccgggatt gcacgtgttt
     7681 cttggtgaac accggaaccg cggcaaatgc tct
//
LOCUS       DATMPU010000370         5319 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_927639,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000370 DATMPU010000000
VERSION     DATMPU010000370.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 5319)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5319)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5319
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_927639"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..499
                     /gene="rpsD"
                     /locus_tag="VNY06_08415"
     CDS             <1..499
                     /gene="rpsD"
                     /locus_tag="VNY06_08415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018044769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HWX14847.1"
                     /translation="RKGKLSDFGTQLKAKQKLKGYYGNISEKQFRKYYFEAIRMRGDS
                     GDHLIGLLERRLDVVVYRAKFVSTVFAARQFVNHGHVRVNGLRVNVASRQVKVGDTIE
                     VKESSRQLIFVLEAQQLAEREVPDYYEVDQSKMTARLNRIPLPSEVPYPVIMEPNLII
                     EFYSR"
     gene            complement(623..1138)
                     /locus_tag="VNY06_08420"
     CDS             complement(623..1138)
                     /locus_tag="VNY06_08420"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018267100.1"
                     /note="Produces ATP from ADP in the presence of a proton
                     gradient across the membrane. Subunit B is part of the
                     membrane proton channel; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP F0F1 synthase subunit B"
                     /protein_id="HWX14848.1"
                     /translation="MFDAEFVVAAGFAIFVGLMIYYGIHKKIAASLDKRGERIAAELA
                     EAARLRSEAEALLASFENRRAEAQAEGEAVIAQAHAEAQRIADEAHARITEFLQRRTK
                     QAEEKIGLAEAQAAAAVRAAAAEAAIKAAEAVLRGEVQGPFGAELIGKSIADLKATSI
                     AVPLLCSDNLG"
     gene            complement(1155..2195)
                     /locus_tag="VNY06_08425"
     CDS             complement(1155..2195)
                     /locus_tag="VNY06_08425"
                     /inference="COORDINATES: protein motif:HMM:NF012645.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX14849.1"
                     /translation="MAKTLRDFSAAATQAIAAGTSHFRSNPEIGESPLLNFAPMLLIA
                     ATLAGAAAALVIVLGFAFLDSPRSAILPLEACLTELARRVQPLESARTALTNRVAAAE
                     AAIAKSASLGNSTFTATQQIEKTLALLPGRNIFSPEKRATELSEFERLENRVLALENK
                     LGLPARREAEMQPAPRAAETSRGETNFPPFDPVNFTPMLVWLAVSFGGLYLLMAKIAL
                     PRVETILQSRTHKIRADIADANKLRAQSEEASAALEKLIADAKSKALTLAQETRAKLL
                     AETESQRVALESELNARLAGSEAQIVEMKARAMSHVETIANEAAAAIVQRITGKPADP
                     SAIANAIVAFKA"
     gene            complement(2315..2545)
                     /locus_tag="VNY06_08430"
     CDS             complement(2315..2545)
                     /locus_tag="VNY06_08430"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020176671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HWX14850.1"
                     /translation="MEPAAARLIGAGLAAIGTGTAAIGVGIIFGQFLLGALRNPTASD
                     GQFGRVFIGVALAEGLGIFALLIAILLLFVVK"
     gene            complement(2600..3340)
                     /locus_tag="VNY06_08435"
     CDS             complement(2600..3340)
                     /locus_tag="VNY06_08435"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020176670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HWX14851.1"
                     /translation="MDPIHQFAIQPIVSLHPFGIDATFTNASLFMVIVSAAVAALMLV
                     GTSSRALVPGRLQSVAEMAYDFVKSTFDMAGLHDGIRFFPFVFTLFLFVLFCNVIGLI
                     PYTFTVTSQIAVTGALALLVIGIVVVYGFMHNGTRFLRLFVPSGVPIALMPFLVPIEI
                     ISFLSRPVSLSVRLFANMLAGHITLKVMGGFVVSLLGAGFFAILAPLPFAMAIILTAF
                     ELLVAVLQAYVFAILACVYLNDAIHPGH"
     gene            complement(3465..3878)
                     /locus_tag="VNY06_08440"
     CDS             complement(3465..3878)
                     /locus_tag="VNY06_08440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012383751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HWX14852.1"
                     /translation="MGERGNGHDGPHLNGQRQRHENENRVRDDDILRVRLEKLTDALA
                     AQHRDERNASATNQGQEGAESSPGALGSVLNLAFRVLSEFIATVIVGAGIGWGLDRVA
                     GTSPIFLIVFLLMGAAAGIWNVYRIGTRKPGADTK"
     gene            complement(4136..>5319)
                     /locus_tag="VNY06_08445"
     CDS             complement(4136..>5319)
                     /locus_tag="VNY06_08445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012592774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HWX14853.1"
                     /translation="AAVAEASAKLQTQLREAETLARRLRGIEEERQSWFARSERAKSQ
                     VGALEERCAEAAQELQKLKEAPELFDSTRQILMLRFEAAEDARRRTADSRAESETRLA
                     NADRAARGALEAMSLAREEKARAEARVEAARERCAEIAHMIATELGCSEAELFGLANI
                     APGEELPAVESVEKRLEGLKNDRERLGGVNLRAEEELNEILASKTNLATEHEDLIEAI
                     KRLRHAIHNLNKEGRDRLLSAFETVQAHFKDLFTTLFGGGSAELQLIESEDPLEAGLE
                     IVVRPPGKKPQIMTLLSGGEQALTAMALIFAIFLTNPAPVCVLDEVDASLDDANVERF
                     CDLLDNMRGRTETRFITVTHNPITMARMNRLFGVTMAERGVSQLVSVDLEEAERFREA
                     S"
BASE COUNT         1140 a         1505 c         1544 g         1130 t
ORIGIN      
        1 ccgcaaaggc aagctttccg atttcggaac ccaactcaag gcgaagcaaa agcttaaagg
       61 ctattacggc aatatttcag aaaagcagtt ccggaaatat tatttcgaag cgatacgcat
      121 gaggggagac agcggcgacc atctgatcgg ccttcttgag cggcggctcg atgtggtggt
      181 ctatcgcgcg aaattcgtgt ctaccgtttt cgctgcgcgc caattcgtca atcacggcca
      241 cgtcagggtc aatgggctgc gggtcaatgt cgcatcccgc caggtgaaag tcggcgatac
      301 aatcgaggtc aaggagagtt cgcgccagct gatctttgtt ctcgaggcgc agcaactcgc
      361 tgaacgcgag gtgcctgact attacgaagt cgaccagagc aaaatgacgg caaggctcaa
      421 ccggattcca cttccttcgg aggtgcctta tccggtgatt atggaaccca atctcatcat
      481 tgaattctat tcgcggtaaa ccgcagatct aacgaatctg tcgtttagag ggtgtccagc
      541 caaaagtggg agccggtttt gcgtctagac acgaaacaaa aatgtagagc aagatgacga
      601 tacgaaggaa agtcatcttt ctctagccga ggttgtccga acatagcaga gggaccgcta
      661 ttgaagtggc cttcaaatcc gcgatgctct tgcctatgag ttcagcgccg aacggacctt
      721 gcacttcacc tcgcagcaca gcttctgccg ctttgatcgc cgcttcggcg gcagccgcgc
      781 gcactgctgc cgcggcttgt gcctcggcca agccgatttt ctcttccgcc tgcttggtcc
      841 ggcgctgcag aaattcggta atccgtgcat gcgcctcgtc agcgatgcgt tgggcttccg
      901 catgggcctg cgcaatgaca gcctcacctt cggcttgcgc ttcggcccgg cggttctcga
      961 aggacgcgag aagggcttcg gcctccgacc gaagccgtgc tgcctcggcg agttcggccg
     1021 caattctttc cccgcgctta tccaggcttg ccgcgatttt cttgtggatg ccgtagtaaa
     1081 tcatgaggcc cacaaaaatc gcaaagcccg ctgcgacaac aaactccgcg tcaaacataa
     1141 aaacggctcc ttaatcaggc tttgaacgcg acaatcgcgt ttgcaatagc cgacgggtcg
     1201 gcgggtttgc cggtgatgcg ctgaacgatt gctgccgccg cttcattggc gattgtctcg
     1261 acatggctca ttgcccttgc cttcatctca acaatctgcg cttccgaacc tgcgagcctg
     1321 gcattcaatt cggattcaag ggcgacccgt tgagactcgg tttctgcaag aagcttggcg
     1381 cgggtctcct gggcaagcgt gagcgccttg gacttcgcat cggcaatgag cttttcaagc
     1441 gcggctgagg cttcttcgga ctgcgcgcgc aatttgttcg cgtcagcgat gtccgcgcgg
     1501 atcttatggg tacggctttg cagaatggtc tcgacacgtg gcaaagcaat tttcgccatc
     1561 aataagtaga gccccccaaa actcaccgcg agccaaacga gcatcggggt gaaattgacc
     1621 ggatcgaagg gcgggaaatt ggtctcgccg cggctggtct cggcggcccg cggagctggt
     1681 tgcatttctg cctcgcgcct tgcgggcaag ccaagcttgt tttccagggc caaaacgcgg
     1741 ttttcaagac gctcgaactc ggaaagctcg gtggctctct tttccggcga gaaaatgttt
     1801 cgtcccggca gtaaagcaag ggtcttttcg atttgttggg tcgcggtaaa ggtcgaattt
     1861 cccaaggaag cactcttggc tatcgcagct tcggctgcgg cgacacgatt ggtaagagcc
     1921 gttcgcgcgc tttcgagtgg ctgtacgcgc cttgccaatt cggttaggca tgcttcgagc
     1981 gggaggatgg cggagcgcgg gctgtccaga aacgcaaacc ccaagacaat tacgagggcc
     2041 gcagcggcgc ccgcaagagt tgcggcaatg agcagcattg gggcgaagtt caacagagga
     2101 ctttcgccaa tttcgggatt tgaacggaaa tgggacgtac ccgccgcaat cgcctgggtc
     2161 gccgccgcgc tgaaatcgcg caatgttttg gccatgctct tgcctcgcga ttgtgcaatc
     2221 gattgcaaat cggctttcac gtcgttaacg ggacgattgt cctcagcgag gcggtggcgc
     2281 ccggagtgag cttccggccg ccgggaatgc attgctattt caccacgaag agcagcagaa
     2341 ttgcgatcag caaggcgaaa atgccaagac cctcggcgag cgcaacacca ataaacacgc
     2401 gtccgaactg accatcggag gccgtcggat tgcgcagcgc tcccaaaaga aactgaccaa
     2461 aaattattcc aacgccgatc gcggctgtcc ccgtaccgat cgcggctagt cctgcaccta
     2521 ttaatctggc tgctgcgggt tccattatgc actccttctg ttgcgctggt gggcgaccgg
     2581 ttgtttttca aatatttatt cagtggcccg ggtggattgc gtcattgagg taaacgcaag
     2641 cgagaatggc gaagacatag gcctggagaa ccgcaacgag caattcgaaa gcggttaaga
     2701 tgatggccat ggcgaaaggc aggggggcga ggattgcaaa aaagccggcg ccaagcaggc
     2761 tcacaacaaa gccgcccatg accttgagcg tgatgtgtcc ggcaagcata ttggcaaaaa
     2821 gacgaaccga aaggctgaca ggacgggaaa gaaaggagat gatttcgatt gggaccagga
     2881 acggcatcaa ggcaatcggc acgccggacg gcacgaagag cctcaggaaa cgtgtgccat
     2941 tatgcataaa tccgtaaacg acgacgatgc caatcacaag cagggcaagc gcgccagtga
     3001 ccgcgatctg gctcgtgact gtaaaagtgt aggggatgag cccgatcacg ttgcaaaaca
     3061 gcacgaacaa aaaaagtgtg aatacaaagg ggaaaaagcg aatgccgtcg tgtaagcccg
     3121 ccatgtcaaa ggtggacttg acaaaatcat aagccatttc ggcgacagat tgcagacggc
     3181 cagggacaag cgcgcgtgaa ctcgtcccaa ccagcatcaa ggcggcgaca gcagccgaga
     3241 cgatcaccat gaaaagagag gcattggtaa aggtcgcgtc gatgccaaaa ggatgcaggc
     3301 tcacgattgg ctggatcgcg aactgatgga tcggatccat tgcattttct tccgccacgg
     3361 cttgttttcc tgctctttgt cccggttcct agcgttgctt cttgaagaca gtctcgggct
     3421 tctcactgcg gaggctcgca atgcggtggt cgccatcgcc tcctctattt cgtatccgca
     3481 ccgggctttc gggtgccaat gcgatagacg ttccaaattc ccgccgctgc ccccatgagg
     3541 aggaaaacga taagaaagat agggctcgtc ccggcaacac ggtccagccc ccacccaatg
     3601 ccggcgccga caatcaccgt cgcaataaac tcgctcagga cgcgaaaggc aaggttcagc
     3661 acactgccca gggctccagg ggatgattcg gccccctctt gcccttgatt tgttgccgat
     3721 gcgttgcgct catcgcgatg ttgtgcggcg agtgcgtcgg tcagcttctc aagtcgcacc
     3781 cgtaaaatgt cgtcgtcgcg tacgcggttc tcattctcgt ggcgttgcct ttggccattg
     3841 agatgcggac cgtcgtgccc gttgccgcgt tcccccattc gctggcgctc ccccgctatc
     3901 cttttgcccg gcccctatag gccatttcgc tgccaaaggc gaggaaggcc aagaccgggc
     3961 gcaatggcga gcttaccgca tagcgttgct ggaggaattc gcggaacttg cgacgcggcg
     4021 cacagtattt acggtggtga gttctgtcaa gatcaaggcc tgaacgccaa agctattgaa
     4081 atagcaccaa taatgcggca agcggattgc gctcggtcgg cgctccaggc agtgttcagc
     4141 tcgcctcgcg gaagcgttcg gcttcttcga gatcgacgct gaccagctgg ctcacgccgc
     4201 gctcggccat ggtcacgcca aacaagcgat tcatgcgcgc catagtgatc ggattatggg
     4261 tcacagtaat gaagcgggtc tcggtcctgc cgcgcatatt gtcgagcagg tcgcagaagc
     4321 gctcgacatt tgcgtcgtcg agcgatgcat cgacttcgtc gagtacgcag accggggccg
     4381 gattggtaag aaatatcgcg aagatcaatg ccatggcggt gagcgcctgt tcgccgccag
     4441 ataaaagcgt catgatttgg ggtttcttcc ccggcgggcg gacgacaatt tcaagtcctg
     4501 cttccagggg atcttcggac tcgatcaatt gaagctcggc agagccgcca ccaaagagtg
     4561 tcgtgaacag gtccttgaaa tgcgcctgaa cagtctcgaa ggcagagaga agcctgtcgc
     4621 ggccctcctt attcaaatta tgaatcgcat gacgcaggcg ctttatcgcc tcgatcaggt
     4681 cttcatgttc ggtggcaaga ttggtcttcg aggcgaggat ctcgttgagc tcttcttcgg
     4741 cgcggagatt aacgcctccg aggcgctccc ggtcgttctt taatccctca agccgctttt
     4801 cgaccgactc cacggcgggc agctcctcgc ccggcgcaat attggcaagg ccgaaaagtt
     4861 cggcttcgga gcatcctagc tccgtcgcga tcatatgcgc gatctcagcg cagcgctctc
     4921 gagcggcttc gaccctggct tcggcgcgcg ccttttcttc ccgcgcaagg ctcatcgcct
     4981 cgagcgcgcc gcgggcagcg cgatccgcat tggcgaggcg ggtttcggat tcggcgcggc
     5041 tatcggcggt gcgtctacgt gcgtcttctg ccgcttcaaa ccgaagcatc aggatctgcc
     5101 gggtggaatc aaatagctct ggggcctcct tgagcttttg cagctcttgg gccgcttctg
     5161 cgcagcgctc ttccaaggca ccaacttggc tctttgcgcg ttcgctgcgg gcgaaccaag
     5221 actggcgttc ctcctcgatg ccgcgaaggc gtcttgcaag cgtctcagcc tcgcgcaatt
     5281 gggtttggag tttggcgctc gcttctgcaa ccgcagcac
//
LOCUS       DATMPU010000371         2720 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_1839299,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000371 DATMPU010000000
VERSION     DATMPU010000371.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 2720)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2720)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2720
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1839299"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..1467)
                     /gene="gcvPB"
                     /locus_tag="VNY06_08450"
                     /pseudo
     CDS             complement(1..1467)
                     /gene="gcvPB"
                     /locus_tag="VNY06_08450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013417727.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
     gene            complement(1461..>2720)
                     /gene="gcvPA"
                     /locus_tag="VNY06_08455"
     CDS             complement(1461..>2720)
                     /gene="gcvPA"
                     /locus_tag="VNY06_08455"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012383742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HWX14854.1"
                     /translation="DELFADIPREKRLEQDLELPPRKTEMEVERLLSGLAARNVAAGS
                     VPFFVGAGAYKHHIPATVDHLIQRSEFLTSYTPYQPEIAQGTLQTIFEFQTQVAALTA
                     MDVANASMYDGSTATVEAVAMARRITKRSRSILSGGLHPHYAEAIRTYSAMAGDDVTV
                     LPPDVFAAENLGALIDSETACVVVQSPDLFGNLRDLSEISAHCETKGALLVAVFTEVV
                     SLGLVKPPGAMGADIVAGEGQSIGNRLNFGGPYVGLFAAKDKYLRQIPGRLAGETVDA
                     LGRRSFVLTLSRREKATSNICTNSGLASLAFTIHLTLLGDAGLWRLARINHANAVDLA
                     CRLAEVRGAEILNETFFNEFTIKVAGDAAHFVERMAEAGVLGGVPVSRLLPGMGLDNL
                     ILTASTEINTDADRAAFVQALDGALSC"
BASE COUNT          593 a          812 c          778 g          537 t
ORIGIN      
        1 ggtgcatgaa aaggcgcgcc aacaaaacgc tcggtttctc cgcgcttggc agctagagcg
       61 agatcgcgca gcgcggcaat gaagagatcc aaagaagcct ttgattccgt ttcggttggc
      121 tcgatcagca tcgccccatg ggccaccagt ggaaaataaa ttgtcatcgg ttgaaagcct
      181 tcatcgatcg aaatcgagcg tcgtgagacc caaaggcttc aaccatgtct cgtcgaacaa
      241 aacctcatgc atgcaggtcc tgtcaccgaa aggctcggac atgagattct tgaggcaggc
      301 ccggacataa ttcgcattga gcacggcgtc ctcggcaacc tggcgcaggc catttgcgcc
      361 atgcgaaagc atataggtga gagcacgcac gaacatgccc atctggccgt gaaacgcagc
      421 gatacggcca aaagactttg gcgcctcgct tcggtgctcg acgagccgga agccatcctc
      481 ttgccggcgc aggaacggca tcggcgcgaa aggcgcgagc cgggatgaaa gcacgaccgg
      541 acccgctccg ggtccgccac cgccatgcgg agtcgagaag gtcttgtgaa gattgatatg
      601 catcgcatca acgccgagat cgcccgggcg cgtcttgccc atgatggcat tgaaatttgc
      661 tccatcgcaa taaaaatagg caccggccgc atgcaccaag gcggcgattt cgacgacatt
      721 tttctcgaaa agcccgcaag tgttggggtt ggtcaacatg agtgcggcga catccggtcc
      781 gagcctgcac ttgacttcgg ccgggtcgat ggtgccatcg gcacgcgccg gcactgggcg
      841 taccgcgaat cccgcgcctg ccgcagacgc cgggtttgtc ccatgcgccg actcaggcac
      901 gaggactaat ttgcgggatt gagcttcccc cttcgcggcg atcgcggcct tgatcaccat
      961 cagcccgcag gcctcgccct gcgcgcccgc ccgtggcggc aacgcaacag cttccatccc
     1021 ggtcagaacc atcagccatc gggcaagttc agcgataagc tcaaatgcac cttgcacggt
     1081 ggattgcggc tgcaacgggt gaatatccgc aaagcctggc aggcgcgcca tcgactcatt
     1141 gagcctcggg ttatgcttca tcgtgcaaga gccgagcggg taaatcccgg catcaattgc
     1201 gtagttgagc cgcgacaatc ttacataatg acgcactgtc tctggctcgc tcaagcccgg
     1261 aagccccggc ggctcgtgcc gctcgagcgc gcccagccga tgtttaaacg gcaagggctc
     1321 gtcaagatcg actccggtat tttctgcgcg cccgatttcg aagatcagcc cttcttcgat
     1381 ttcgagaccg cggttgccgg tgaaagttcg ggtctcctgc gcctctacac cggctgcttt
     1441 taggaccggc tcctgcatca tcagcatgac agtgctccgt caagggcttg cacaaaggct
     1501 gcgcgatcgg catccgtatt gatttccgtc gaagctgtca gaatgaggtt gtcgagtccc
     1561 attcctggca acaaccgcga gacgggaacg ccgccgagca cgccggcctc tgccatccgt
     1621 tcgacgaaat gcgcggcgtc gcccgcaact ttgattgtga attcgttaaa gaacgtctcg
     1681 ttgagaatct cggcgcccct tacttcggcg agccggcaag cgaggtcgac cgcattggcg
     1741 tgattgatgc gcgcaagccg ccatagtcct gcgtcgccaa gcagcgtcaa gtggatcgtg
     1801 aaggcaaggc tcgcaagacc cgaattggtg cagatgtttg acgttgcctt ttcgcgcctg
     1861 gaaagggtca aaacgaagct gcggcgcccc aaggcatcga ctgtttcgcc cgcaagacgt
     1921 ccaggtatct gacgcaaata tttgtccttc gcagcgaaaa gcccgacata gggaccgcca
     1981 aaattcagcc gattgccaat ggactgtcct tcgccggcga caatatctgc tcccatcgct
     2041 ccgggtggct tgacaaggcc aagcgataca acctcggtga aaaccgcgac gagcagcgcg
     2101 cctttcgttt cgcagtgcgc ggagatctct gaaagatcgc ggagattgcc gaaaagatcc
     2161 gggctctgca ccacgacgca agcggtttca ctatcgataa gcgctcccag gttctcggcc
     2221 gcaaaaacat ccggcggaag cactgtgaca tcatcgccag ccatagccga ataggttcgg
     2281 atcgcttcgg cataatgcgg atggagcccg ccagataaaa tcgatcttga gcgtttggta
     2341 atgcggcgcg ccatcgccac ggcttccact gtggcggtgg atccgtcata catcgacgca
     2401 ttggcaacat ccatcgcagt taaagccgcg acctgggtct ggaactcgaa aatggtttgc
     2461 agtgtgcctt gcgcgatctc tggctgatag ggcgtgtagc tcgtgagaaa ttcggaacgc
     2521 tggatcagat gatcaacagt tgcgggaata tggtgtttgt aggctcccgc tccgacaaaa
     2581 aatggcaccg aaccggccgc cacatttcgt gccgcaaggc cggaaagcaa acgctccacc
     2641 tccatttcgg tcttgcgtgg cggcagttcg aggtcttgtt cgagccgctt ctccctcgga
     2701 atatcggcaa aaagttcatc
//
LOCUS       DATMPU010000372         7911 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_2451071,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000372 DATMPU010000000
VERSION     DATMPU010000372.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 7911)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7911)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7911
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2451071"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..272
                     /locus_tag="VNY06_08460"
     CDS             <1..272
                     /locus_tag="VNY06_08460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012383077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="coproporphyrinogen III oxidase"
                     /protein_id="HWX14855.1"
                     /translation="CPLGDEEAFAALFAFTRKMGTHFLSIYPEIVRRNLSVAWTKVDR
                     EEQLIRRGRYAEFNLLYDRGTIFGLNTGGNTEAILSSLPPLARWR"
     gene            complement(423..995)
                     /gene="rplI"
                     /locus_tag="VNY06_08465"
     CDS             complement(423..995)
                     /gene="rplI"
                     /locus_tag="VNY06_08465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012589941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HWX14856.1"
                     /translation="MEVILLERVAKLGQMGEIVRVKDGYARNYLLPGHKALRATDINK
                     ARFETERTQLEARNLETKNEAAAIAEKLNGQSFVIVRQAGETGHLYGSVSPRDIAAAV
                     TDDGFSIGRNQVVLKAPIKSLGLHEIPVHLHPEIEAAITINIARSAEEAERQAKGEAV
                     TAVAETTLEDLGLDEIGAALAEAGGSLGEH"
     gene            complement(1019..2173)
                     /locus_tag="VNY06_08470"
     CDS             complement(1019..2173)
                     /locus_tag="VNY06_08470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX14857.1"
                     /translation="MEKQQAVLYESAALLELWPVPYITLTAFRAGLQDMKIRRLLKFA
                     PAAGLGIGAGVAAAILFSLAGRGTPFAAALANLSPLPIMIAMLGFGLVAGAASVVSAT
                     LTVAGLFYARQKFGHVDAAALAGLTFSFFIGLPALWLSLLSILSRAKGSPNWVVTTRV
                     GSFFSREYCPLDRVLSYTTSISATIGVAIAISISTLYGGFDLALEKLTAEIVPVLEPL
                     LSRVHLPPGVDARYLAETVVLAAAPAAAGGSLMMMMLNLWFAGRVVQLSGQLPRPWPD
                     IAGELRLPRNFLLVFGAASVVGPYFGGLPGLIVITIAATIGVAFALVGLAVAHFVTRG
                     LSIRTPLLILVYLLLVITWPLPLLVLLLIGVLETAFSLRDRKDQAASPRK"
     gene            complement(2255..2497)
                     /gene="rpsR"
                     /locus_tag="VNY06_08475"
     CDS             complement(2255..2497)
                     /gene="rpsR"
                     /locus_tag="VNY06_08475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012589939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HWX14858.1"
                     /translation="MTTAAAPRRPFFRRRKSCPFSGVNAPKIDYKDIRLLSRYISERG
                     KIVPSRITAASAKKQRELARAIKRARFLGLLPYVIR"
     gene            complement(2497..2910)
                     /gene="rpsF"
                     /locus_tag="VNY06_08480"
     CDS             complement(2497..2910)
                     /gene="rpsF"
                     /locus_tag="VNY06_08480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014890781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HWX14859.1"
                     /translation="MALYEHIYLARQDISGQQVDALTAQFKSVIESFGGKVEKVEYWG
                     LRSLAYRIKKNRKAHFSLLNIDAPTAALAEMERQMGINEDVIRFITVRVDALEQGQSA
                     MMRKRDDDDRGRGGRGERAPRRARDETMEIEEANF"
     gene            complement(3145..3567)
                     /locus_tag="VNY06_08485"
     CDS             complement(3145..3567)
                     /locus_tag="VNY06_08485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018408820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX14860.1"
                     /translation="MLAIPGKACGECTLCCKILEITELSKPAGTLCEHCIKSASGGCS
                     IYATRPKVCRAYECLWKGDRAMSPQLRPDRVGTLLMEDPDSDEYRAVCDPGKPLAWLT
                     PLIFKHLVAMAKSGRTVVAKAGLKAWRVHGNGQWGPCA"
     gene            complement(3730..4410)
                     /gene="gmk"
                     /locus_tag="VNY06_08490"
     CDS             complement(3730..4410)
                     /gene="gmk"
                     /locus_tag="VNY06_08490"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019401503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HWX14861.1"
                     /translation="MARNNPAAVAEPLAAQALQRRGLMLILSSPSGAGKTTLTRMLLQ
                     TKELDLTLSISVTTRQRRSSEVDGIHYTFISKAKFEQLRNGGELLEWAEVHGSFYGTP
                     REPVEEVLRQGRDMLFDIDYQGTRQVKEKCAGDAVTIFILPPSMQELRARLERRAEDR
                     REIIEARLHEARNEIRRWAEYDYVLVNDDLQRVFRELCAIRLAERRRTERARKGIEAF
                     AETLLTEI"
     gene            complement(4416..5300)
                     /locus_tag="VNY06_08495"
     CDS             complement(4416..5300)
                     /locus_tag="VNY06_08495"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014891665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HWX14862.1"
                     /translation="MTLASMTGFARATGDAGPWHFAWELKSVNAKGLDLRLRLAPPFD
                     AIEPEARGKIAGRLARGTVHATLIADREPMVQKVRINQDLLRELIGAVTWIALPEKIA
                     PATLDGLLAVRGVVEISDAADDDATILQARASALDLLGTVLDNFLATRQNEGRDLSIF
                     LSARLERMAALATAAELSPARKPEATRARLAHSLALLAGNASFDQNRLHQEALMLAAK
                     ADIREELDRLIAHITAARELLAKGSPTGRRLDFLAQELSREANTLCAKSNDASLTKIG
                     LELRVEIEQFREQVQNIE"
     gene            complement(5378..6292)
                     /gene="era"
                     /locus_tag="VNY06_08500"
     CDS             complement(5378..6292)
                     /gene="era"
                     /locus_tag="VNY06_08500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003608971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HWX14863.1"
                     /translation="MTSENDAGTSCGFVSLIGAPNAGKSTLINRLVGTKVSIVSRKVQ
                     TTRSCIRGIVIEGRSQIIFVDTPGIFAPRRRLDRAMVTAAWEITGNTDAAVLLVDARK
                     GVDTEVEAIVAKLQLIAVTRILALNKIDTIEPPKLLELAATLNDELSFAQTFMISALR
                     GFGLAKLKQELAALMPPGPWLYPDDQFSDAPLRTLAAEITREKLLDRLHDELPYASTV
                     ETTLWQDLPDGSARIEQTIYVQRESHKKIVIGEGGRTIKAIGIAARKDIIEAAGQKVH
                     LFLFVKIRENWADDSERYHQLGLTFPKE"
     gene            complement(6289..6999)
                     /gene="rnc"
                     /locus_tag="VNY06_08505"
     CDS             complement(6289..6999)
                     /gene="rnc"
                     /locus_tag="VNY06_08505"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012385403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HWX14864.1"
                     /translation="MARLDLNVKALEARIGHVFTNRALIAAALTHVSAAAGGKRETYQ
                     RLEFLGDRVLGLVISEMLFNSFPEADEGELSRRLAGLVRKESCAEIARVWGVDSIIRL
                     GEGEHPATAAKATILGDVCESIIGAVFLDAGYEAAKAVVTAAFSEEMRAPARPLRDSK
                     TELQEWAQARGLPPPLYRETARSGPDHAPEFTIRVEVPGYPDAEAKGFAKRLAEQAAA
                     AAFIAREGIGRGSQKGAA"
     gene            complement(7001..7795)
                     /gene="lepB"
                     /locus_tag="VNY06_08510"
     CDS             complement(7001..7795)
                     /gene="lepB"
                     /locus_tag="VNY06_08510"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020176754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HWX14865.1"
                     /translation="MTEPVQVNRKGTEMKQQAKEGGKGDTLKVIVQALLIALVVRTFL
                     FQPFNIPSGSMIPTLLIGDYLFVSKYAYGYSDHSFPFSPNLFSGRIFGSPPKRGDVVV
                     FKLPRDGQTDYIKRVIGLPGDNVQMKDGRLYINGEIVPRQPIARVRTEGFYGGEAEVP
                     TYQETLPGGVVHTIIQIQGDTGFNSNTQVFEVPSGQYFMMGDNRDNSTDSRVPPDQGG
                     VGFVPFENLVGHAEMIFFSVGKGEPAWEFWKWPWTARWDRIFRIVK"
BASE COUNT         1673 a         2300 c         2266 g         1672 t
ORIGIN      
        1 attgccccct gggcgacgaa gaagcattcg ccgcattgtt tgccttcacg cggaagatgg
       61 gcacgcattt tctttccata tatcccgaaa ttgtacgccg gaatttgtcc gttgcttgga
      121 ccaaagtcga tcgcgaggaa cagctcatcc ggcgcgggcg ctatgctgaa tttaacctat
      181 tgtatgatcg cgggacgatt ttcggactaa acaccggcgg caataccgaa gcgattttgt
      241 cgtcgctgcc gccgctggcg cggtggcgct gagaaggttt aagcgatcct agccttaaag
      301 cggggaaagt atccagttcg aaccctcggg gcaacgactt tgagttgaat cgtccccttc
      361 tctaagtccg tgtctcgtcg cgtatccggg cgcttaatcg gtctcaattt tgcttgacac
      421 gattagtgct ccccgaggct tccacctgcc tcggcaagcg ccgcgccgat ttcgtcgagg
      481 cccagatcct cgagggtggt ctctgcgacg gcggtaacgg cttccccttt agcttggcgt
      541 tcggcctcct cggcggagcg cgcgatattg atggtaatcg cggcttcgat ctcgggatgc
      601 aaatgtaccg ggatctcgtg caaaccgagc gacttgattg gcgctttcag gacgacctgg
      661 ttgcggccga tgctaaagcc gtcatccgtg accgcggcgg caatatcgcg cggcgaaacc
      721 gagccataga gatgacctgt ctcgccggcc tgccggacga tgacgaagct ttggccgttg
      781 agcttttcgg caatggcagc cgcttcgttc tttgtttcaa gattgcgggc ttcaagctgg
      841 gtacgttctg tttcaaaacg ggccttattg atgtcggtcg cgcgaagtgc tttgtgcccc
      901 ggcagcaggt aatttctggc gtagccatcc ttcacgcgga cgatttcacc catctggccg
      961 agcttggcca cacgttcgag caaaataact tccatgctct gatcctttgg cgggggaatt
     1021 actttctagg cgaggctgcc tgatctttgc gatcacgaag tgagaatgcg gtttctaaca
     1081 cgccgatcag gagcaagacg agaagaggga gaggccaggt aataaccaga agaagataga
     1141 ccaggatgag aaggggggtg cggatactca atccgcgcgt aacaaaatgc gccacggcaa
     1201 gcccaacgag ggcgaaggcg acgccgatcg tcgctgcaat agttatgaca atgaggccag
     1261 gaagtccgcc aaaataggga cctaccacgg aggcggcgcc gaaaaccaag agaaaattcc
     1321 gcggcaacct aagttcgcct gcaatgtcgg gccacggcct cgggagctgc ccggaaagct
     1381 gcaccacccg tcccgcgaac caaagattga gcatcatcat catgaggctt ccgcccgccg
     1441 cagctggagc ggccgccaaa acgacggtct ccgccaaata gcgcgcgtcg actccgggag
     1501 ggaggtgcac gcggctgagc agtggttcca gaaccgggac aatttcggcg gtcaattttt
     1561 caagcgcgag gtcaaatccg ccataaaggg tgctaatcga aatcgcgatc gcgactccga
     1621 tagtggcaga gatggaagtt gtatatgaaa ggacccggtc aagcgggcaa tattcgcgag
     1681 aaaaaaagct gccaaccctt gtcgttacca cccaattcgg gcttcccttc gcccggctca
     1741 aaatcgagag gaggcttagc cacaaggcgg gcaagccgat gaagaaagag aaagtcagac
     1801 ccgcaagcgc ggccgcgtcg acatggccga atttctgacg cgcatagaac agtcccgcga
     1861 cggtgagcgt ggcggagaca accgacgcag cgcccgcaac gaggccaaat ccaagcatcg
     1921 cgatcatgat gggcagtggc gacaaattgg cgagcgccgc cgcaaaaggc gtcccgcgac
     1981 cggcgaggct gaacaaaatg gcggccgcaa ctcccgcccc gatcccaagg ccagcggctg
     2041 gagcgaattt caagaggcgc cttatcttca tgtcctgcaa tcccgcgcga aaggcggtaa
     2101 gggtaatgta aggaacaggc catagctcca gcaaagccgc gctctcatat agcacggctt
     2161 gctgcttttc cactcgaggg ccgcaatatt agagcaattc tattccgctg ggaatatccc
     2221 gcggccaacc acgcgcagag ctctgcctgc gaaatcagcg gatcacatag ggcaatagcc
     2281 cgagaaacct tgcgcgtttg atagccctcg cgagttcgcg ctgctttttg gccgaagctg
     2341 cagtgatccg cgatggaaca atcttcccgc gctcggagat gtagcgcgag aggaggcgga
     2401 tgtccttata atcgatctta ggcgcgttca caccggaaaa cgggcaggac ttgcgccgac
     2461 ggaaaaaggg cctgcggggg gcggcagctg ttgtcatcag aagttcgcct cctcgatttc
     2521 catggtctcg tcgcgcgccc gcctaggagc tctttcgccg cgcccgccac ggccgcggtc
     2581 gtcgtcatcg cgcttgcgca tcatcgccga ctggccttgc tccaacgcgt cgacccgaac
     2641 cgtgatgaag cgtatgacgt cctcattgat acccatttgc cgctccattt cggcgagcgc
     2701 ggcggttggc gcatcaatat tcaagagcga gaaatgcgcc ttgcgattct tcttgattcg
     2761 gtaggcgagg gaccttaacc cccaatactc aaccttttcg accttgcccc cgaaggattc
     2821 gattactgac ttgaactgtg ccgtcagggc gtccacttgc tgtccggaaa tgtcctgacg
     2881 tgccaaatag atatgctcat aaagagccat ggggctgcct ttccttgttc cacgctcgcg
     2941 attgatcggc gctgagccct tcgagcctgg taaggctcgt tgagactaac gaaggcgggg
     3001 acaccgggaa gcggatcaca aaaatcctac ccacgaaacg ggctcccgtt gagccccagg
     3061 ccggaggcgc gaaggcggct ttatacaagg aatttttccc caggcaaggc tggtgcacaa
     3121 aacgggtgga ccgccagaca aaaatcaagc gcaaggaccc cactgcccat tgccatgcac
     3181 acgccacgct ttcaatcccg ctttggcgac aacagtccgg ccgcttttcg ccatcgcgac
     3241 gagatgtttg aagataagcg gggtgagcca ggctaagggt tttcccggat cgcagaccgc
     3301 gcgatactca tcggaatcag gatcttccat caaaagcgtg cctacgcgat ccgggcgcaa
     3361 ttgcgggctc atcgcgcgat cgcctttcca caaacattca tacgcgcggc aaactttcgg
     3421 tctcgttgca tagatgctgc agccgccaga ggctgacttt atgcaatgtt cgcaaagtgt
     3481 gccggcgggt ttcgacaatt ccgtaatttc aagaatcttg cagcataaag tgcattcgcc
     3541 gcaggctttt cctgggattg caagcatgaa ctcctcaccg ccgaggctga ttcgatcttg
     3601 agactctcta tcccgatagg cattggcgca aggaaaatga tatattgaag cgtcctgctt
     3661 gcccttgcgc agactacgtc gccgagtcga gttcaaaccg tgcgtgagat ctgagtttaa
     3721 gcacgccgat catatttcag taagcaacgt ttccgcgaaa gcttcgatcc ctttcctggc
     3781 gcgttctgta cggcgacgct cagccagacg gatcgcacaa agctcgcgga acacgcgttg
     3841 caagtcatca ttgacgagca catagtcata ctccgcccag cggcggattt cattgcgggc
     3901 ctcatgcaac cgggcctcga tgatttcccg ccggtcctcg gcgcggcgtt cgagtcgcgc
     3961 gcgcaattcc tgcatcgagg gtggcaagat aaaaatggtg acggcgtctc cagcgcattt
     4021 ttctttcacc tgccgtgtgc cctggtaatc gatgtcgaag agcatgtcgc gcccttgcct
     4081 caagacctct tcgacaggct cacgtggtgt gccgtaaaaa ctgccatgca cctctgccca
     4141 ttcgagcaat tcgccaccat tgcgcagctg ctcgaacttg gccttgctaa tgaaagtgta
     4201 gtggatcccg tcgacttcgc tcgaacgccg ctggcgcgtg gtcacggaga tggaaagcgt
     4261 caaatcgagc tctttggtct gaagtagcat ccgagtcagg gttgtcttgc ctgccccgga
     4321 gggggaagaa aggatcaaca tcagtccgcg ccgttgcagc gcttgagcgg ccagcggctc
     4381 cgcgactgcg gcagggttat tgcgcgccat gattgctact caatattttg gacctgctct
     4441 cggaactgct cgatctcgac gcgcaattcg agtcctatct ttgtcaggct cgcgtcgttc
     4501 gatttggcgc agagggtgtt ggcctcacgg gaaagttcct gggcgagaaa gtcaagcctc
     4561 cggccggtcg ggctgccttt ggccagaagc tcgcgggcgg ctgtgatgtg cgcgatgaga
     4621 cggtccaact cttctcggat atcggccttg gccgccagca tgagcgcctc ctggtgcaga
     4681 cggttttggt cgaaactggc attgccagca agcagagcaa gcgagtgtgc gagcctcgcc
     4741 cttgttgcct ccggcttgcg ggccggagag agttcggccg ccgtcgccaa tgcagccatg
     4801 cgttcaagcc gcgccgatag aaatatcgaa aggtcacgac cctcgttttg ccgcgtcgca
     4861 agaaagttat ccagcactgt gccgaggaga tcaagggcgc ttgcccgcgc ctgaagaatc
     4921 gtggcatcgt cgtccgcggc gtcagagatt tccaccacgc cccgaacggc aaggaggccg
     4981 tcaagcgtcg cgggcgcgat cttttcaggt agggctatcc aggtaacggc gccaattagc
     5041 tcccgcaaca aatcctgatt gatgcggact ttttggacca tcggttcgcg gtccgcaatc
     5101 agtgtcgcat gcacagttcc gcgcgcaagc cttcccgcga tttttccgcg cgcctctggc
     5161 tcgattgcat caaagggagg cgcaaggcgg agcctcaaat cgagaccctt ggcattgacc
     5221 gatttgagtt cccaggcaaa gtgccatggc ccggcatctc cggtggcccg ggcaaagccc
     5281 gtcatgctgg caagtgtcat tgcataacgc gcaatctgga gggtagattt ctctctacaa
     5341 taacgccggc cacaaagcgg ctcaagcgaa gcgcgattca ttccttagga aaagtcagtc
     5401 ccaattggtg gtaccgttcc gagtcatcgg cccagttttc gcgtatttta acgaataaaa
     5461 acagatgcac cttttgtccg gccgcttcga tgatgtcctt gcgtgcggct atcccaatcg
     5521 ctttgatcgt tcggccgcct tcgccgatca cgattttctt gtggctctcg cgctgcacat
     5581 aaattgtctg ttcgattcgc gccgacccat ccggcagatc ttgccagagc gtcgtttcga
     5641 ccgtcgatgc gtaagggagc tcatcatgca gccgatctaa aagcttttcg cgcgtaatct
     5701 cggcggctag cgtccgtagt ggcgcgtccg aaaactgatc gtccggataa agccacgggc
     5761 ccggcggcat aagtgcggcg agttcttgtt ttaacttggc gagcccaaac ccgcgcagcg
     5821 ccgaaatcat aaaagtttgc gcgaaggaaa gttcgtcatt gagcgtggcg gcaagttcga
     5881 ggagttttgg cggctcaatc gtgtcaatct tattgagagc gaggatcctt gtgacggcga
     5941 tgagctggag tttggcaaca atggcttcga cttcggtgtc gacgcctttg cgcgcatcca
     6001 ccagcaggac cgccgcgtcg gtattgccgg ttatctccca ggccgctgta accatcgccc
     6061 ggtcgagcct gcgccttggc gcgaaaattc cgggtgtgtc gacgaaaatg atttgcgacc
     6121 ggccttcgat gacaatgccg cgaatgcagc tgcgtgtcgt ttgaaccttg cgcgaaacga
     6181 tggagacttt tgttcctacg agccggttga taagcgtcga tttcccggca ttgggtgcgc
     6241 ctatgaggga gacgaagccg cagctcgttc cggcatcatt ttcgctggtc acgcggcgcc
     6301 tttttggctg ccgcggccga tgccttcacg cgcgatgaag gctgccgcag cggcctgctc
     6361 ggcgaggcgc tttgcaaatc ccttagcctc ggcgtccgga tagcctggca cttcgacgcg
     6421 gatcgtgaat tctggcgcat gatcgggtcc tgatcgcgcc gtctcgcggt aaagcggcgg
     6481 cggcaggcct cgcgcttgtg cccattcttg cagttctgtc ttcgaatcgc gcaaaggacg
     6541 cgcgggcgcg cgcatctctt cggaaaaagc agcggtcacc accgccttcg ctgcctcata
     6601 acccgcatca aggaaaaccg cgccgatgat cgattcgcac acatcgccca ggatggtcgc
     6661 cttggcggca gtcgcgggat gttcgccttc tccaagccgg ataatggaat cgacccccca
     6721 gacgcgggca atttcggcgc agctctcttt gcggacaagg cctgccaagc gccgcgaaag
     6781 ctcgccttca tcggcctctg ggaaggaatt aaaaagcatc tcggaaataa ctaggcccaa
     6841 gacgcgatcc ccaaggaatt cgagtcgttg ataagtttca cgcttgcctc ctgctgccgc
     6901 gctcacatgg gtgagcgccg ccgcaatcag agcccggttg gtgaagacat gcccgatgcg
     6961 ggcctcgagc gctttgacgt tcaggtctag ccgcgccatc ctatttcacg attctgaata
     7021 tcctgtccca gcgcgccgtc caaggccatt tccagaattc ccaagcgggc tcccccttgc
     7081 cgaccgaaaa gaaaatcatc tcggcatggc ccacgagatt ttcaaatgga acgaaaccca
     7141 cgcccccctg gtccggcgga acacggctat ccgtcgaatt gtcacggtta tcgcccatca
     7201 taaagtactg tccggacgga acttcgaaaa cttgggtatt gctgttgaat ccggtgtcgc
     7261 cttgaatctg aatgatcgtg tgcacgacac cgccaggcag ggtctcctga taggtcggaa
     7321 cctcggcctc gcctccgtag aaaccttctg tacggaccct ggcgattggc tggcgcggca
     7381 caatttcgcc gttgatataa aggcggccat ccttcatttg cacgttgtcg cccggcagcc
     7441 cgatgacgcg cttgatgtag tctgtttgcc cgtcgcgcgg gagcttgaac acgacaacgt
     7501 cgccgcgctt tggcggcgag ccaaaaatcc tgcccgaaaa caaattggga ctgaatggaa
     7561 aggaatgatc cgagtagcca taggcatatt tggagacaaa aaggtaatca ccgatcaata
     7621 aagtcgggat catggagccg gatggaatat tgaatggctg aaacaagaag gtgcgcacga
     7681 cgagagcgat gagcagcgcc tggacaatga ccttcaaagt gtcgcccttg ccgccttcct
     7741 ttgcttgctg cttcatttcg gtgcctttac gattcacctg cactggctct gtcatgtttg
     7801 attcttcccg caaaaacacg cggcggatcg gattttaagc aacgcgccgc acgctaaggc
     7861 gcgctcctta gcatgcggcg tcaggccggt ataggctcgc tctgcagcgg c
//
LOCUS       DATMPU010000373         4528 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_2452595,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000373 DATMPU010000000
VERSION     DATMPU010000373.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 4528)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4528)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4528
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2452595"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            380..835
                     /locus_tag="VNY06_08515"
     CDS             380..835
                     /locus_tag="VNY06_08515"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020175292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HWX14866.1"
                     /translation="MPETGNTLTLETTKGPVVIELRPDLAPAHIQHIKRLAGVRFYDG
                     VAFHRVIEGFMAQTGCPHGTGTGGSKYPNLKAEFNAQPHVRGTCSMARSQGKNSANSQ
                     FFICFANARFLDKRYTVWGKVTSGMENVDKIKRGEPVRDPDRIISATIS"
     gene            914..2005
                     /gene="queA"
                     /locus_tag="VNY06_08520"
     CDS             914..2005
                     /gene="queA"
                     /locus_tag="VNY06_08520"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012385302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HWX14867.1"
                     /translation="MRVDLFDFELPKERIALRPAEPRDSARLLVVRPHAVPQFEDRSI
                     LDLPNYLAPGDVLAVNDTRVIRARLHGVRSRGETSVRVEATLIRQEGEGLWRAIARPA
                     KRLKPGDIVHFAAVNGRGCVAEALDAEVLQKGEEGDVTLKFALSGAALDAAIEQIGQM
                     PLPPYISSRRSTDEKDTQDYQTLFAQKPGAVAAPTASLHFTPRLLAVLAARRISIQQV
                     TLHVGAGTFFPVKADDTTNHKMHAEWGEVTDEAATRFNKTRASGQRIIAAGTTSLRIL
                     EQAARADRTFSAFSGETSLFITPGYKFKAVDMLLTNFHLPRSTLFMLASAFTGLETLK
                     AAYAHAIAERYRFYSYGDACLLFPAEKWA"
     gene            2002..3135
                     /gene="tgt"
                     /locus_tag="VNY06_08525"
     CDS             2002..3135
                     /gene="tgt"
                     /locus_tag="VNY06_08525"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014893074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HWX14868.1"
                     /translation="MSEAFRFEILGQDKTARTGVITTPRGQIRTPAFMPVGTAATVKA
                     MHPEAVKALGADIVLSNTYHLMLRPGPERIAALGGLHKFMNWPWPILTDSSGFQAMSL
                     SKLRKLDENGVTFHSHIDGALYRLTPERAMEIQDLLDSEIKMQLDECVRLPCSESEVR
                     RAMELSLAWAERSKRALVAKEGRALFAIVQGGADEALRVESAKELANIGFDGYALGGL
                     AVGEPRPIMLAMIECTLPFLPSDKPRYLMGTGTPDDLLESVRRGIDMFDCVLPTRAGR
                     HGLAYTRAGRLNLRNARHADNPAPVDPLASCAAARTYSRAYLHHLVKSGEILGMMLLT
                     EINLSYYQDFMADMRRAIAAKRFDDFCVATKAAWRIGEASGAL"
     gene            3247..4080
                     /locus_tag="VNY06_08530"
     CDS             3247..4080
                     /locus_tag="VNY06_08530"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR04325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase, TIGR04325 family"
                     /protein_id="HWX14869.1"
                     /translation="MGAKQLVKKLAPRVWSALGALQGNSAHRGPNKHREPDEWRVTFE
                     GRYATWGEVAALTGADAFPVASKDYRASLRTSTDDWDGYFKHHEVGLLAAVMSAMAST
                     RSPQKTVVDFGGGYGVHFLTMLKANLIQCQWEVVELRDVAQLGTQKWRHNRNIQFIET
                     LESATYRPDLVVASGVFQYLADPYASIRQIEALEPSFLFVDRLPMINEDRDVYTVQRI
                     GAAFFGVERVVPHVLFSRNRLVAELTRDFDLVVRFQGFQDPSHEDISYEAIFLRRRAS
                     S"
     gene            complement(4126..>4528)
                     /locus_tag="VNY06_08535"
     CDS             complement(4126..>4528)
                     /locus_tag="VNY06_08535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012592516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HWX14870.1"
                     /translation="LPQGEATLFLPSGSSGPAFLLSANYWILKAYNNSDSYALSLALL
                     AERIEGKSELRDRWPEREVFLSRTQKAELQRYLEKLGFYHDTIDGRFGQASRDAIHDF
                     QKSIGTAPADGFATPDLLRRLAVEADRRPGR"
BASE COUNT         1002 a         1262 c         1298 g          966 t
ORIGIN      
        1 aggttcgcta gagggaacat aagtcgcatc cgcttgcgcg acggcggctg ccacgaccaa
       61 cagggcggca ataacaaaat ggaatggcat aaggtgctga gccttgctca tgtccgccat
      121 gattccctcc caggcatttt cttgagatca gcgttggacc gctgctttct cgaaatccta
      181 atattgcgcg cttgtcggcc cgagcgcgtc ctctgaatag gggatgccaa tctcaaggcc
      241 gctgcaatat aaatgcttag ctttggctgc aagcggttat ttgtcaattc cgcgggcgcg
      301 acgtgtcggc agggctagca ggtttacctc gcccctttca tcggataagg cagacgggga
      361 agctaaccag gagaacggca tgccggagac cggtaacacg ctcaccctcg agaccacaaa
      421 aggaccggtc gtgatcgaat tgcggcctga ccttgcaccc gcccatatac agcatatcaa
      481 gaggctcgcc ggcgtaagat tttatgacgg cgtcgctttt catcgcgtga tcgagggttt
      541 tatggcccag accggctgcc cgcatggaac cggaaccggg ggctcgaaat atcccaatct
      601 caaagccgag ttcaatgccc agccgcatgt gcgcggcaca tgttccatgg cccgctcaca
      661 gggcaaaaat tccgccaact cacaattctt catctgtttt gctaacgcac gttttctcga
      721 taagcgatat acagtctggg ggaaggtcac gtccggcatg gaaaatgtcg acaagatcaa
      781 gcgcggcgaa ccggtgcgtg atcctgacag aataatttcc gccacgatca gctgaccgcc
      841 gaagcatgcc cgcggctgga gtgggttgcc tgctgggagg ggccatcact gtttcgctgg
      901 cagtcgccgc attatgcgag tcgatctctt cgattttgaa ttgccaaaag agcgtatcgc
      961 tctgcgcccg gcggaaccac gcgactctgc acggctgctt gtcgttaggc cacatgcggt
     1021 tccgcaattc gaggaccgta gcatcctcga tctgccaaat tatcttgcgc ctggcgatgt
     1081 gctcgccgtc aacgatacgc gagtcatacg cgcgcggctt catggcgtgc gaagccgtgg
     1141 cgaaacctcg gtgcgagtcg aggcaaccct gatccggcag gaaggcgagg gactatggcg
     1201 cgctattgcg cggcccgcga agagattgaa gccaggtgat atcgttcact ttgcggccgt
     1261 caacggccgg gggtgcgtcg ccgaggctct cgacgccgag gttctccaaa agggtgagga
     1321 gggagatgtt acgctaaaat ttgcactttc tggcgcggcg ctcgacgcgg caatcgaaca
     1381 aataggccag atgccgttgc ccccttatat ttcaagccgt agatccaccg acgagaagga
     1441 tacgcaagac tatcagacac tgtttgcaca aaagccgggc gcggtagcgg cgccaacagc
     1501 gagcctccat ttcacgccaa ggcttttggc tgtcctcgct gcgcggagaa tctcaattca
     1561 acaagtaacg ctgcatgttg gcgccggcac attttttccg gtcaaagctg acgacaccac
     1621 aaatcacaaa atgcacgccg aatggggcga ggtcacagac gaggccgcca ctcgctttaa
     1681 caagacgcgg gcatccggac aaaggatcat tgcggcaggg acaacgagcc tcaggatcct
     1741 cgaacaagcg gcgcgcgccg acagaacctt ctctgctttt tcgggcgaaa catcgctctt
     1801 catcacgcca ggctataagt tcaaggcggt tgatatgctg ctcaccaatt tccatctgcc
     1861 gcgctcaaca ttgttcatgc tcgcctctgc tttcacaggg ctcgaaacgt taaaggcggc
     1921 ttatgcgcat gcgatcgcgg agcgctatcg gttttacagt tatggcgatg cctgcctgtt
     1981 gtttccggcg gagaaatggg cgtgagtgaa gcattccgtt ttgaaattct gggtcaggac
     2041 aagacggctc gcacaggagt tatcaccacg ccgcgcggac aaattcgcac gccggctttt
     2101 atgccggtcg gcacggccgc caccgtcaaa gcgatgcatc ccgaagccgt gaaggcgctt
     2161 ggcgccgata ttgttctttc taacacctat catttgatgc tgcgtccggg tcctgagcga
     2221 atcgctgcgc ttggtggact gcataaattc atgaattggc cttggccaat cttgaccgat
     2281 tccagcgggt ttcaagccat gtcgctttcg aagctaagaa agctcgatga gaatggcgtg
     2341 acctttcact cccatatcga tggcgcgctc tatcgcttaa ccccggaacg ggcgatggag
     2401 atccaggacc tgctcgattc cgaaataaag atgcagctcg acgaatgcgt acggcttcct
     2461 tgctcggaat cagaagtccg ccgggcgatg gaattgtcgc tcgcctgggc ggaacgctcg
     2521 aaaagagcct tagttgcaaa agaaggccgc gccttgttcg ccattgttca aggtggcgcg
     2581 gatgaagcct tgcgcgtgga aagcgccaag gagctcgcga atatcgggtt cgatggctat
     2641 gcgctcggcg gtcttgccgt tggtgagcca aggccaatca tgctggccat gatcgaatgc
     2701 acgctgccat tccttccctc ggacaaaccg cgctatctca tgggtacggg aacgccggat
     2761 gatcttttgg agagcgtcag gcgaggaatc gatatgttcg attgtgtgct gccgacgcgc
     2821 gccgggcgcc atgggcttgc ctatacgcgg gctggaaggc tcaatttgcg caacgcccgc
     2881 cacgcggaca atcccgctcc agtcgatccg cttgcgtcct gcgcggctgc caggacttat
     2941 tcccgcgcct atcttcatca tctcgtcaaa tcgggcgaga tcctcggcat gatgcttttg
     3001 accgagatta atctttctta ttaccaggat tttatggcgg acatgcgccg tgcaattgca
     3061 gccaagcggt tcgacgattt ttgtgttgct accaaggcag catggcggat cggcgaggca
     3121 agcggtgcgc tctaggcgtg gtcagatcgt tgaccttggc gcgagatctg ataattcgtc
     3181 acgccacaga tcgcgctaaa gtaaaccctg accaacagcg cggggatacc ttttcctgag
     3241 cacgcgatgg gcgcaaagca actggtaaaa aagcttgctc cacgcgtttg gagcgccctc
     3301 ggtgcgctcc aagggaactc ggcgcatcgc gggccaaaca agcaccgcga gccagatgaa
     3361 tggagggtca cattcgaagg gcgatatgcc acctggggcg aggtggccgc gctgacaggc
     3421 gcggacgcct tcccggtggc gtcgaaagac taccgtgcct cactgcgcac cagcaccgat
     3481 gattgggacg gatacttcaa acatcacgag gttggcttat tggcggccgt gatgtcagcc
     3541 atggcctcca ctagatcgcc gcagaagaca gttgtggatt tcggcggcgg ctatggcgtg
     3601 catttcctta ctatgctcaa ggctaacctc atccaatgcc aatgggaagt cgtcgagcta
     3661 agagatgtgg cacagcttgg gacacaaaag tggcgacaca acagaaacat tcagtttatc
     3721 gaaacgctag agtcggcaac ctaccgccca gatttggtgg tagcaagcgg ggtgttccaa
     3781 tacctagcag acccttatgc ctcgatcaga caaatcgaag cgctggagcc gtccttcctg
     3841 tttgtagacc gtctaccgat gatcaacgaa gaccgcgatg tttacacggt tcaaagaatc
     3901 ggagcggctt tcttcggtgt ggaaagggta gtaccgcacg tcctcttttc acgtaacagg
     3961 ctagttgctg agctaacgcg cgatttcgat ctagtagttc gattccaagg ctttcaggat
     4021 ccgtctcatg aggatattag ttatgaagcg atcttcctta gacgacgggc ctccagctaa
     4081 tgagcctgac ttgccgccca cgccgcggaa tgatcagcta gttgtttacc ggccaggccg
     4141 gcggtccgct tccactgcaa gacggcgcaa aaggtccggc gtcgcaaagc cgtcggccgg
     4201 agcagtgccg atgctcttct ggaaatcatg gattgcgtcg cgcgaggcct gcccgaaacg
     4261 gccgtcgatg gtgtcatggt agaacccgag cttttccaga taacgttgta gttccgcctt
     4321 ttgtgtgcgg cttaaaaaaa cttctcgctc aggccaccga tcacgcagct cagacttgcc
     4381 ttcaattcgc tcggcaagga gagcaagcga cagggcgtag gaatccgaat tgttgtaggc
     4441 cttaaggatc caataatttg ccgacaggag aaaggctggg ccagacgatc cagagggcaa
     4501 gaataaagtg gcctccccct ggggcaaa
//
LOCUS       DATMPU010000374         2931 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_302574,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000374 DATMPU010000000
VERSION     DATMPU010000374.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 2931)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2931)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2931
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_302574"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(52..507)
                     /locus_tag="VNY06_08540"
     CDS             complement(52..507)
                     /locus_tag="VNY06_08540"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012592050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030612 - arsenate reductase (thioredoxin)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase ArsC"
                     /protein_id="HWX14871.1"
                     /translation="MKPAVKNRPQAVLFACTRNAVRSPMAEALGRQLFGKEVYFASAG
                     VKKGDKNGFAVAVMDEAGIDMSKHRPHTFEDLEDSYFDLIVTLSPEAHHKSLDFTCTL
                     ATEVVYWPTIDPTAVEGSRERVLDAYRDVRDHLRERIKKLLDYQPMGNL"
     gene            complement(649..1137)
                     /locus_tag="VNY06_08545"
     CDS             complement(649..1137)
                     /locus_tag="VNY06_08545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014892941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0262 family protein"
                     /protein_id="HWX14872.1"
                     /translation="MTRKDSNDRNRLVSVTLDEASIGRGAADQEHERQIAIYDLIDEN
                     SFVLPGRDSGPYGLNIALQDAKLALEVIDESGTRLVTHILSLTPFRGLLKDYFLICES
                     YYAAIRTAMPRQIEAIDMGRRALHDEAADLLVERLKGKIECDRDTARRIFTLVAALHW
                     KG"
     gene            complement(1134..2435)
                     /gene="hisD"
                     /locus_tag="VNY06_08550"
     CDS             complement(1134..2435)
                     /gene="hisD"
                     /locus_tag="VNY06_08550"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020177037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HWX14873.1"
                     /translation="MPLKLDTQNPDFEASFEALLAMKREVAEDVDQAVQAIIADVIAR
                     GDAALFAYSRKFDRADVEKQGLRVGADEIDAAFGCCSREALEALRFAHERIIGFHNRQ
                     VPQDLRYADPLGVELGWRWLPVEAAGLYVPGGTASYPSSVLMNAVPAKVAGVRRLVMA
                     VPAPDGHLNPLVLVAAKLAGVDEIYRIGGAQAVAALAYGTESIAPVTKIVGPGNAYVA
                     AAKRRVFGAVGIDMIAGPSEVLIIAGDGARPQWVAADLLAQAEHDAAAQSILITNDKV
                     FADAVEDEVVRQLQTLPRGEIAGASWRDFGAIIVVNNLEESAELANRIAPEHLEIMTT
                     NAEALAQHIHNAGAIFIGPYTPEAIGDYVGGTNHVLPTARSARFSSGLSVLDFMKRTS
                     ILKCSADSLAALGKAAITLGQAEGLNGHARSVALRIDPRAT"
BASE COUNT          667 a          838 c          782 g          644 t
ORIGIN      
        1 acgcttcttc aatagctcga tgcgatttgc aaggagcttg cagtaattta ttcatagatt
       61 tcccatcggc tggtaatcga gcaacttctt gatgcgttcg cgtagatgat cgcgaacatc
      121 gcgatatgcg tcaagaacgc gctcgcgcga cccctctaca gcagtcggat ctatggttgg
      181 ccaatagacg acttcggttg cgagggtgca ggtgaaatcg agcgatttat gatgggcttc
      241 gggtgaaagg gtgacgataa gatcgaaata agagtcctcg agatcttcga atgtgtgggg
      301 gcgatgcttg ctcatatcga ttccagcctc atccataacc gcgactgcga atccattttt
      361 gtctcccttt ttgacacccg cagatgcaaa gtagacctcc tttccaaaaa gctgccgtcc
      421 gagggcttcg gccattggcg agcgcacggc gtttcgcgtg caagcaaaga gcacggcttg
      481 cgggcggttt ttcacggcgg gcttcatttg ggcggaaacg agagcttgtc cggcaaaacg
      541 gggaccgctt ttgcgtccgg acgagcgaca aaacgaagag ttagcgcaag aggacgactc
      601 gaagaaaagt catcttgcgc tagacgcctt ggggcgccgg tatgggattc aacccttcca
      661 atgcaatgcg gcgacgaggg taaatatccg ccgtgcggta tcgcgatcgc actcaatctt
      721 gcccttaagc cgctcaacaa gaagatccgc tgcctcgtca tgcaaagccc ggcgccccat
      781 gtcgattgct tcgatttgcc tcggcatggc agttcggatc gccgcgtaat agctttcgca
      841 aatcaaaaaa tagtccttca ggaggccgcg aaatggagtc aaagacaaaa tatgcgtcac
      901 gaggcgcgtg ccgctttcat caatgacctc caaagcgagc tttgcgtcct gcaaggcgat
      961 attgagccca tatggccctg agtcgcgccc tggcagcaca aaactgtttt cgtcgatgag
     1021 gtcgtagatc gcgatctgcc gctcgtgctc ctgatccgcc gctccccggc cgatcgatgc
     1081 ctcgtcgaga gtaacggaga caaggcgatt acgatcattc gaatcctttc gggtcatgtc
     1141 gcccgagggt caattcgcag cgccacagag cgcgcgtggc cattcaatcc ttcggcttga
     1201 ccgagagtga tcgctgcttt gccgagcgcc gccaaactgt ccgcactgca cttcaaaatg
     1261 gaggtccgtt tcataaaatc gagtacgctc aagccagacg aaaaccgcgc tgaacgcgcg
     1321 gtcggcaaga catggtttgt gcctccaaca tagtcgccga tggcctcggg cgtataaggt
     1381 ccaataaaga tagcaccagc gttgtgaata tgttgcgcga gcgcttcggc gttcgttgtc
     1441 atgatttcga gatgttccgg ggcaatccgg ttggcaagct cggcactctc ttcaaggtta
     1501 ttgacaacga taatagcgcc aaaatcacgc cagcttgccc cagcgatttc accacgggga
     1561 agggtttgca attgccgcac cacttcatct tcaacggcat ccgcaaagac cttgtcattg
     1621 gtgatgagaa tcgactgagc agcagcatcg tgctctgcct gcgcaaggag gtcggctgcg
     1681 acccattgag gcctagcacc atctccggca atgatcaaaa cctcggaggg cccagcgatc
     1741 atgtctatgc cgacagcacc aaagactctt cgtttggcag cggcgacata ggcattgccg
     1801 ggaccgacga ttttcgtgac cggcgcaatg gattcggttc catatgccag cgcggccacc
     1861 gcttgcgcgc cgccgatgcg gtaaatctca tcgaccccag caagcttcgc cgccaccaag
     1921 acaaggggat tgagatgtcc gtccggcgcc ggcacggcca tgaccaagcg gcggacgcca
     1981 gcaaccttgg ccggtaccgc attcatcaat accgatgacg gatagctcgc cgtgccgcca
     2041 ggaacataaa gccccgccgc ctcgactggg agccagcgcc agccgagctc aacgcccaac
     2101 ggatcagcgt agcgcagatc ttgcgggacc tggcgattat gaaagccaat aattcgttca
     2161 tgtgcgaagc gcaatgcctc gagcgcttcg cgggagcagc acccaaacgc ggcgtcgatt
     2221 tcgtctgcgc caacccgcaa accttgtttt tcgacgtcag cccgatcgaa ttttcgggaa
     2281 taggcaaaaa gggccgcgtc gcccctcgcg atcacatcag cgatgatggc ttgaactgcc
     2341 tggtcgacat cctcggccac ttcccgcttc atcgcaagaa gcgcttcaaa tgacgcctca
     2401 aaatccgggt tttgcgtgtc gagcttcaac ggcatgaatg cgccttctta atgtttcgag
     2461 caaacctggc ccgctggagg gtcattttgc aacgggcaac ttgcgggctc ttagccaccc
     2521 gtcaattgct tttggcagtt ctcagagcag cttctcaatg tgcttttcaa gggcgccacc
     2581 gcggaactta aacgagcggc aacctcgccc atagatcgac aaggaatttc gtgaagcttc
     2641 atttgattgc cccggttccc agctaccggt agagcgcctc aagccctctg tattgcggcg
     2701 caaatccacc tttgcttgtt gtgtttacca ccaacagcag ctttccgaat tagtcgggag
     2761 aggcttttcc cttccgtcca gagtcccggc cgaaaaactc agaaatagtg ctcattacat
     2821 tctccggatg acgtcatgtc tcttttgact gatgagaaat atcgataatc atttgtaatc
     2881 atacttctgt cggggaagct agcccgcaac acatagctat agctatgtca a
//
LOCUS       DATMPU010000375         4302 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_2453171,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000375 DATMPU010000000
VERSION     DATMPU010000375.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 4302)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4302)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4302
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2453171"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..750
                     /locus_tag="VNY06_08555"
     CDS             <1..750
                     /locus_tag="VNY06_08555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HWX14874.1"
                     /translation="IVGEIKDITLRNQYQLEFKERFFRLFRRSANRQLSGTPNVVRAL
                     PQGILAEAPPVSTRLAASPLFRTGKASLSPREGLILLLLLNHPGLISRYVEELAEVAF
                     SSEDATALRDAMIAASDGCSGHATPGDVPSAPSTGGPPIRAILENRGFAGILQKLDAT
                     AAHGSHWYAKAEAAEADAEEVLKQALSLHRRAKALHRELQLAELALGKDSSETNLGRL
                     KDIQAQLSALGGIEAAMEGFGAQSGHPRGTL"
     gene            1103..3154
                     /gene="rpoD"
                     /locus_tag="VNY06_08560"
     CDS             1103..3154
                     /gene="rpoD"
                     /locus_tag="VNY06_08560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012590645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HWX14875.1"
                     /translation="MAKKDSENSDVERGAVEVADGPLLDLTDASVKRMIKLAKKRGFV
                     TYEELNAVLPSEEVNSEQIEDVLAMLNEMGINVVEHEEQDEGEEGASEEEEVESGDII
                     EASQSKAATARASEPADRTDDPVRMYLREMGSVELLSREGEIAIAKRIEAGREAMIAG
                     LCESPLTFQAIIIWRDELNDSKVLLRDIIDLEATYAGPDGKNTPKIDMTAPGAAEALA
                     AAQQAPAIAPIEPPARLRPASEEGEPDATSREESFDEEDDMENSVSLSAMEAELKPKV
                     VDTFDRIASTYKRLRRLQDQNVVNKLKNETLTPSQERKYKTLKKEIVRDVKSLSLNPN
                     RIEALVDQLYDINKRLIGLETKLLRMAEGTGVGREDFLKNYQNSELDPKWLLRVSKLG
                     GRGWKDFVARKKDEVKELLSEIHGLATETGLEIQEFRKIVHLVQKGEREARQAKKEMV
                     EANLRLVISIAKKYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQA
                     ITRSIADQARTIRIPVHMIETINKIVRTSRQILHEIGREPTPEELAEKLAMPLEKVRK
                     VLKIAKEPVSLETPIGDEEDSHLGDFIEDKNAILPIDAAIQSNLRETTTRVLASLTPR
                     EERVLRMRFGIGMNTDHTLEEVGQQFSVTRERIRQIEAKALRKLKHPSRSRKLRSFLD
                     N"
     gene            3700..4008
                     /locus_tag="VNY06_08565"
     CDS             3700..4008
                     /locus_tag="VNY06_08565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011472595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX14876.1"
                     /translation="MKPYVPFSYRKCLAAGFAALTVGSGLLATPASAGGSDLAVGLLG
                     GLAAGTVVGSAIAQPRYYYYPPYYAPAPVYAPAPACWYERRSVWDGYGYVIQPVRVCQ
                     "
     gene            4120..>4302
                     /locus_tag="VNY06_08570"
     CDS             4120..>4302
                     /locus_tag="VNY06_08570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX14877.1"
                     /translation="MLLETALKIREARIDRLALSLDTSESDKWACVLLMALMAQIGVA
                     SVHLNGARAQFAATMIV"
BASE COUNT          973 a         1197 c         1263 g          869 t
ORIGIN      
        1 atcgttggcg aaatcaaaga tataaccctt cgcaaccaat atcagctaga atttaaggag
       61 cgtttctttc ggttgttccg gcgctccgcc aatcgtcaac tttcggggac gccaaatgta
      121 gttcgggcat tgccccaggg cattctcgcg gaggcgccgc cggtcagcac gaggttagca
      181 gcatcccccc tgttccgcac ggggaaggcg agcctttcgc cgcgcgaagg gctaatttta
      241 ttgcttcttc tcaatcatcc gggactgatc tcgcgctatg tcgaggaatt ggctgaggtc
      301 gcgttttcga gcgaggatgc gacagcgtta cgtgacgcga tgattgcggc ttcggacgga
      361 tgttcgggtc acgcaacccc tggcgacgtg ccttccgcgc ctagcacagg ggggcctcct
      421 attcgtgcca tcctcgagaa ccgaggtttc gccgggatat tgcaaaagct cgatgccacc
      481 gccgcgcatg gatctcattg gtacgccaaa gcggaagccg ccgaagcgga tgcggaggag
      541 gtcttgaagc aggccctgag cttgcatcgc cgcgccaagg cgctacatag ggagttgcaa
      601 ttggctgaat tggctctagg taaggactct agcgaaacca atctcggccg tctcaaagat
      661 attcaggcgc aattgtcggc gctcgggggg atcgaggccg caatggaggg ctttggtgcg
      721 caatccggac acccaagggg aacgctgtga gcagctgcgg gggcgggtcg agccaaaagt
      781 tagaccggtt ttgcgcccgg gcacgcggca aacgaagttt agggcgagat gacgatttgg
      841 gcgaaagtcg tcgcgctctc ttaggagcgc tggcggttcg agcgccttcc ccccggtccg
      901 ctaccggcac aggggtacgc aatcggatgg gtgaacagtc gacacaatgc acgtgaaatc
      961 aatgtgaata ttgtcaacga ccgaggggcg cttttccggg ctgcgccgcc tataggcaag
     1021 cgggcccggg gagagaccgg ccgcagttca caagccgagg ctgcagccct tttacaattt
     1081 cccgcatttc cggagaccat tgatggcgaa aaaagacagt gagaattccg atgtggaacg
     1141 cggcgcggtc gaagtcgccg atgggccgtt gctcgatctc acggatgcct cggtcaagcg
     1201 gatgatcaaa ctcgccaaga agcgggggtt tgttacctat gaggaattga atgccgtgct
     1261 tccgtccgag gaagtcaatt ccgagcagat cgaagatgtt ttggcgatgc tgaacgaaat
     1321 gggcatcaat gtcgtcgagc atgaggaaca ggacgagggg gaggagggcg cgtccgagga
     1381 agaagaagtc gagagcggag acatcatcga ggcttcgcag tctaaggccg ccacggcgcg
     1441 cgcgtccgaa cccgcggatc gcaccgatga ccccgtgcgg atgtatctgc gcgagatggg
     1501 ctcggtcgag cttctgtctc gcgaaggaga aatcgccatt gccaagcgta ttgaagctgg
     1561 ccgcgaggcg atgattgccg ggctttgtga aagccctctc accttccagg caatcattat
     1621 ttggcgcgac gaattaaacg acagcaaggt actgctgcgg gacattatcg atctcgaagc
     1681 gacctatgcg ggtccggacg gaaaaaatac tcccaaaatc gatatgacgg cgcccggtgc
     1741 cgccgaggcg ctcgctgccg ctcaacaggc cccagcgatc gcgcccatcg aaccgccggc
     1801 ccggttgcgg ccggcaagcg aggagggcga gcctgatgca actagccggg aagagtcctt
     1861 cgatgaagaa gatgatatgg aaaattccgt ctctttgtcg gcgatggagg cggaactcaa
     1921 acccaaggtc gtcgatactt tcgaccggat agcgagtacc tacaaaaggc tccggcgcct
     1981 gcaggatcaa aacgtcgtca ataagcttaa gaacgaaacg ctcacccctt cgcaggaacg
     2041 aaagtacaaa acgctcaaga aggaaatcgt gcgcgatgtc aaatcgctgt cgcttaatcc
     2101 aaaccggatc gaggccctgg tcgatcagct ctatgacatc aacaaacggc tgatcggtct
     2161 tgagacgaag ctattgcgga tggccgaggg cacgggtgtt gggcgcgaag actttctcaa
     2221 gaattatcag aattccgaac tcgacccgaa atggcttttg cgcgtgtcga agctcggcgg
     2281 ccgcggctgg aaagatttcg tcgcccgcaa gaaggatgag gtcaaggaac tgctttcgga
     2341 gattcacggg ctcgcgaccg agacgggtct cgagattcag gaattccgca agattgtcca
     2401 tctcgtgcaa aaaggcgaac gggaggcgcg gcaggccaag aaggaaatgg tggaggcaaa
     2461 tcttcgcctc gtaatttcga tagccaagaa atacaccaat cgcggcctgc agtttctgga
     2521 tctgatccaa gaaggcaata ttggcctcat gaaagccgtc gataaattcg aatatcggcg
     2581 cggttacaaa ttctcaacct atgcgacctg gtggatccgg caggcgatca cccgttcgat
     2641 cgccgatcag gcccgcacca ttcgcattcc ggtgcatatg atcgagacaa tcaacaagat
     2701 tgtgcgcacc tcgcgccaga tattgcatga aatcggacgc gagcccacgc ccgaggagct
     2761 tgccgagaag ctcgccatgc cgcttgaaaa agtgcgcaag gttttgaaga tcgccaagga
     2821 gccggtctcg ctcgaaacac cgatcggcga cgaggaagat tctcatctcg gcgatttcat
     2881 cgaagataag aatgcaatcc tgccgattga tgccgcgatc caatcaaacc ttcgcgagac
     2941 aacgacgcgc gtgcttgctt ctttgacgcc gcgcgaggag cgcgtgttgc gtatgcgctt
     3001 cgggatcggc atgaacaccg accacacgct ggaggaagtc ggccagcagt tctcagtgac
     3061 acgcgaacgc atccgccaga tcgaggcaaa ggcgcttcgc aagctgaaac acccgtcacg
     3121 ctcgcgcaaa cttcgcagtt ttttggataa ttgaaaaggg tgcgtcttac ggctttgacc
     3181 ggggccgtgc cggcaaagtc gtacgacctg ctcagaatgg ccatagagtc tatcggcgtg
     3241 tctgtcgcca accgaagtac ggcgtttcca ttggcgagta cccgtcttcc gattgatccc
     3301 gtagcgcttg gcgagaaccc tcaagccgac ggaccgcctt tgtcgtgccg gcgctcccat
     3361 gcaaaacctg ccctccgcga gccattccaa ctggctctgc cagccggaac cattcgctgg
     3421 cttcgcccat agtgcatcct ttgattctcg cgataagagt gcagcatcaa atcctgacct
     3481 agagccatgg catgcgtggg caatattttc ttaacgctgc gcgcccaagg aatcagcaat
     3541 gtttaacttt ttgaggacac attgacctgc cacctcaaag aaacgtccct ttggagccgc
     3601 cacgtccttg ttgggcctga catgattacg cccagaaatg acgcgacaat tatcttcgct
     3661 ggaattgagg ttccgggaaa agatttaagg atgcatccca tgaagcccta tgtccctttt
     3721 tcgtaccgga agtgccttgc cgccggtttt gctgcgctta cagtcggctc tggcctcttg
     3781 gcgacacccg catcagctgg tggaagcgac ctcgccgtcg gcttgctcgg cggtctggcg
     3841 gcaggcactg tcgtgggcag cgccatcgcc cagcctcggt attactacta cccgccctat
     3901 tacgcgccgg cgccagtcta tgcccctgct cccgcatgct ggtatgaacg gcgcagcgtg
     3961 tgggacggct acggatatgt catccaaccc gttcgcgtct gccagtaatg cgtcccgatt
     4021 gtcgaaaact ccattagacc gcgccgagcc aaagcaagaa ccggatttgc gtcccgacac
     4081 gctacaatct acaattttgc gggcttcgcc ctcgaccgga tgttgcttga aacggctttg
     4141 aagatcaggg aggcccgaat tgaccggctg gcattaagcc ttgatacttc cgaaagcgat
     4201 aaatgggcat gcgttctttt gatggcccta atggcgcaga ttggcgttgc ctcagtgcac
     4261 cttaatggag cgcgcgccca attcgcggcg acaatgattg tg
//
LOCUS       DATMPU010000376         8990 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_4307106,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000376 DATMPU010000000
VERSION     DATMPU010000376.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 8990)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8990)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8990
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4307106"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(695..2176)
                     /gene="gatB"
                     /locus_tag="VNY06_08575"
     CDS             complement(695..2176)
                     /gene="gatB"
                     /locus_tag="VNY06_08575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017486316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HWX14878.1"
                     /translation="MDNKPRKLTKGATGDFEVVIGMEVHAQITSKTKLFSGASTEFGK
                     EPNSNVSLIDAAMPGMLPVINEECVAQAVRTGLGLKAKINPRSVFDRKNYFYPDLPQG
                     YQISQYKSPIVGEGSVTVDISPTEQVEVGIERLHIEQDAGKSLHDQSATDSFVDLNRS
                     GVGLMEIVSKPDLRSAEEAKAYVAKLRTILRYLGTCDGNMEQGSLRADINVSVRRPGE
                     PLGTRCEIKNVNSIRFIGQAVEAEARRQIGIIEDGGKIDQETRLFDPGRGETRSMRSK
                     EEAHDYRYFPDPDLLPLEFDQAYVDALAGYLPELPDTKKARFIREYGLPPYDAGVLVA
                     DKDTADYFEAAAAYGGTPRDAKLVANWIIGDVAAFANSQALSVPQTHIMPGQIATIVD
                     LIAEGTISGKIAKDVLAAIMAEERYADPRAIVEARGMKQVTDQALIEAYVDAIIAINP
                     DKVAQARVKPSMLGWFVGQVMKETDGKASPRTVNDILKAKLGI"
     gene            complement(2476..3261)
                     /locus_tag="VNY06_08580"
     CDS             complement(2476..3261)
                     /locus_tag="VNY06_08580"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HWX14879.1"
                     /translation="MQRMFSRRLAGATAGLLLTATSPFAADLAPPPPPPPPVPVFTWT
                     GFELGLHVGGGVGRTSVDLLNFSNSYSTSGPFGGLHLGYNYQFTGPIVIGLQAEYNFA
                     GITGSRTGIPLNFVSIDIQQFGSVDGRLGLAFDRLLIYGIGGFAYADIRNHILCGQFQ
                     CDGFPTSIFGPVTRDFAANRYGFDVGGGIEYNLWGNWTARAEYRFYRWNSLTFVDAGF
                     GAFGNPFNIAIPNHRDGEYLHTGRIGLTYKFAWPYATPVVARY"
     gene            4160..5170
                     /gene="fabD"
                     /locus_tag="VNY06_08585"
     CDS             4160..5170
                     /gene="fabD"
                     /locus_tag="VNY06_08585"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012385266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HWX14880.1"
                     /translation="MPLASNKKSYIRDRKLRAFIFPGQGSQAAGMGKALADAFPQARA
                     TLREVDEALGQNLSLLMFEGPQAELTLTANAQPALLAVSLAVVRVLEAEAGLNLARDA
                     RFVAGHSLGEYSALAAAGSLSVGEAAKLLRVRGLAMQEAVPVGAGAMAALIGLDCEAA
                     QAIAERAAMEAGDGVICEVANDNGGGQVVVSGSVIAVHRAVELAKEQGARRSISLPVS
                     APFHCSLMQPAAEAMAEALVSAAIKPPCVPIVSNVRAAPVTNPEEIRKLLIAQVTGTV
                     RWRESIVFMAGAGISCFVECGAGKVLSGLVKRIATSATSISIGVPDDIGRLSSDIHCG
                     SF"
     gene            5174..5911
                     /gene="fabG"
                     /locus_tag="VNY06_08590"
     CDS             5174..5911
                     /gene="fabG"
                     /locus_tag="VNY06_08590"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003608266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HWX14881.1"
                     /translation="MFDLTGKTALVTGASGGLGRAISRALHDQGAVVGLSGTRGDALG
                     ALASELETNVHVFPCDLAEKSATEALIPAVEGAMGSIDILVNNAGITRDNLFLRMTDE
                     EWETVLAVNLTAAFRLSRACLKGMVRRRHGRIIGISSVVGVTGNPGQGNYCAAKAGMI
                     GMYKSLAAEVARRNVTVNCVAPGFIESPMTDALNDKQKYAILATVPTGRLGTGDDVAA
                     AVVYLASAQASYVTGQTLHINGGMAMI"
     gene            6087..6326
                     /locus_tag="VNY06_08595"
     CDS             6087..6326
                     /locus_tag="VNY06_08595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007559070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HWX14882.1"
                     /translation="MSDVAERVKKIVVEHLGVDADKVIDSANFMEDLGADSLDTVELV
                     MAFEEEFGVEIPDDAAETIVTVGDAIKFLEKASAA"
     gene            6511..7800
                     /gene="fabF"
                     /locus_tag="VNY06_08600"
     CDS             6511..7800
                     /gene="fabF"
                     /locus_tag="VNY06_08600"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012385263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HWX14883.1"
                     /translation="MRRVVITGLGLVSPLGGGVEPSWERLLSGQSGARRIENFDVSDL
                     ACKIAMPVPRGGEGNFEPDQWMEPKEQRKVDDYILYAVAAATQALADAGWKPSTYEEQ
                     INTGVLIGSGIGGLGGIAETAIVLKEKGPRRVSPFFIPGRLINLAGGYVSIQHGLKGP
                     NHAVVTACSSGTHAIGDAARLIALGDAEVMVAGGTESAINRIGIAGFAACRALSTKFN
                     DQPKRASRPYDKDRDGFVMGEGAGCVVLEAYEHAMARGAKVYAELAGYGMSGDAYHIT
                     APDESGDGACRAMAAAIKRAGISPGDIDYINGHGTSTPLGDEIEIRAVEKVVGNDRHS
                     LSMSSTKSAVGHLLGAAGAVEAIFSILAIRDQIVPPTLNLDQLSVETEIDLVQHTAHK
                     REVKFVLSNSFGFGGTNACLVFKRPERPGPAVPRSST"
     gene            8048..>8990
                     /locus_tag="VNY06_08605"
     CDS             8048..>8990
                     /locus_tag="VNY06_08605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003608260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HWX14884.1"
                     /translation="MPSNLNGNDVPDRDEESLREEQQQSGRFVGQRVTPRSPKEALQP
                     DTPPPPPPIPPSRRRPVLSALSGFLSFLLAMAIAAVSGLIWSQHQLHEPGPLSTSRVV
                     YIAPGTEVPDIIAALERDGVIDSPFLLNATLLVEGNRSKVKAGEYLFKQNASLRDVID
                     TLVSGKQVLHAITIPEGLTSEQIVERLRENDILIGDVIDIPKEGSLLPETYKVSRGTL
                     RADVIRKMQEDQRRTVDQIWARRAANLPLRSPYELITLASIVEKETGKADERPRVASV
                     FLNRLRKGMRLQSDPTIVYGLAGGNGAFGRGITRSELE"
BASE COUNT         1949 a         2497 c         2591 g         1953 t
ORIGIN      
        1 actctttttc gccgcggttc tctttactgc tttcttggtg gttgccttca ccatgttcaa
       61 tattcctttg ctgatttagc cgactgaatc gaaatgtcga ctagatgctt gcatagggta
      121 ccgctgttaa tcagccatgc aagaggcgct gtttcgtaga gactgcaaat cagggcaaat
      181 aacgtaccgg cagcatgtct agaagtgctt atgaccttgc tcgctgcaaa gccgtgttag
      241 ctgcaagttg gtgacctctc tcgtcgaata agggcgcccg catataccac cgaatgaatg
      301 ctgctaagtg gcgaccgcaa gcgtaatgcg gtagcggaca cgatctcgtc agcccactgg
      361 ctgattcaaa aatccattat atttaaaggc tctattctat ttttatcgcg ccaatgcaaa
      421 ctcgcttatc aacgctgctt gcgcggacca tcgcaattgc tcacgatcag ttcgcggctg
      481 tgattgcaaa caggataggc tattttaggg atcaatttcg cgcctggatc gagttgtccg
      541 accgatgaca agagtaccga ttgacggcgc tgacggacga taaaaaaaaa ttcgttggaa
      601 gagaaaaaaa tcgccgctta aaggctacgc aatcgttcca atcgacgcat ctcgcgcgaa
      661 tcacccgaat cacttgatga ttcgcggcga caatttagat cccaagtttt gcctttaaga
      721 tatcattgac agtacgagga ctggcctttc cgtcggtctc cttcatgacc tggccgacaa
      781 accagccgag catagagggc ttgacccgcg cctgggcaac cttgtctggg ttaatggcaa
      841 tgatcgcatc gacgtaggct tcgatcagag cctgatcggt aacctgcttc attccgcgag
      901 cctcgacgat ggcacgtgga tcggcgtacc tttcttccgc catgatggcc gccaacacgt
      961 ccttggcaat tttaccagaa atcgtgccct cggcgatgag atcgacgatc gtggcaattt
     1021 gccctggcat tatgtgggtc tgcggaaccg acagcgcctg cgaattggca aaggcggcca
     1081 cgtctccaat gatccaatta gcgacaagct tggcgtcccg aggagtgccg ccataggcag
     1141 ccgccgcttc gaagtagtca gccgtatcct tgtcggcgac gagcacgcct gcatcataag
     1201 gcggcaggcc atactccctt ataaagcggg ccttcttggt atccgggagt tcgggcaaat
     1261 agcccgccaa ggcatcgaca taggcttgat cgaattccag cggaagaagg tctggatcgg
     1321 gaaaatagcg gtaatcatgc gcttcctcct tgctgcgcat cgaccgggtt tcgccgcgcc
     1381 ccggatcgaa tagccgggtt tcctgatcga tcttgcctcc atcttctata ataccgatct
     1441 gacgacgcgc ctcggcctcg accgcctgtc cgatgaatcg tatcgaattg acgtttttga
     1501 tttcgcaacg cgttccgaga ggctcgcccg gccggcggac agacacatta atgtccgccc
     1561 gtagcgatcc ctgctccatg ttcccgtcgc aggtgcccaa gtagcgcaga attgtgcgca
     1621 gcttcgctac ataggctttg gcctcttctg cagagcgcag gtccggtttc gacacaattt
     1681 ccatgaggcc gacgccggac cggttgagat cgacgaaaga atcggttgcc gactggtcat
     1741 gcagagactt gccggcatcc tgctcgatat gcagacgctc gatgccaacc tcgacctgtt
     1801 ccgtcggcga gatatcgaca gtcacgctcc cttcgcccac gatggggctc ttatattggg
     1861 aaatctggta gccttggggc agatcgggat agaaatagtt cttacggtcg aagaccgaac
     1921 gcggattgat cttcgccttg aggccaaggc ccgttcgtac cgcctgggct acgcattcct
     1981 cattgatgac aggaagcatc cccggcatgg cggcatcaat aagcgatacg ttgctatttg
     2041 gctccttgcc aaattcagtg gaagcacctg aaaatagttt cgtcttcgat gtgatttgag
     2101 cgtgaacctc catcccaatc acaacctcaa agtcgcccgt cgccccttta gtaagcttcc
     2161 tcggtttgtt gtccattgct tcgcctattg ccgcgcgttc tctgctgcac cataactccg
     2221 cgcgctcggc aagcgaagac ccgaagcggc ggaaattggc agtggcgccg ccgcgctgcg
     2281 gattattttg ccgaggcgtg cgttctcggg ggcaaacaat agcgcaaacc tcgtgccggc
     2341 cgccccgctc aaagcctgca ttggatgcgg ccaagatact ttacgcgaac aaaacgccgt
     2401 tacggcaggt taaaccgtaa cggcgctcat ggcatcggcg aaaccccagg caggagctta
     2461 ggccgacaac attactcagt agcgggcgac caccggagtg gcgtagggcc aggcaaactt
     2521 gtaggtcaga ccgatgcggc cggtgtgcag gtactcccca tctctgtgat tgggaatggc
     2581 aatgttaaaa ggattgccaa acgcgccgaa cccagcgtcg acgaaagtga gcgagttcca
     2641 cctgtagaac cggtactcgg cccgcgccgt ccaattgccc cagaggttat attctatacc
     2701 gccgccgaca tcgaagccgt agcggttggc tgcgaagtca cgggtaactg ggccaaaaat
     2761 tgacgttgga aagccatcgc attggaattg accgcaaaga atatgattac ggatgtcggc
     2821 ataggcaaag ccgccaatcc catagatgag caggcggtcg aacgcaaggc cgagacgccc
     2881 gtcgaccgat ccgaactgct ggatgtcgat ggacacaaag ttaagaggta tgcccgttct
     2941 gcttccggtg atgccggcga aattatattc ggcctgcagg ccgatgacga tcggtcccgt
     3001 gaactgataa ttgtagccga ggtgaagacc gccgaacgga cctgacgtac tgtacgagtt
     3061 ggaaaagttt aagaggtcga ccgaggtgcg gccaactcct ccgccaacat ggagccctag
     3121 ttcaaagccg gtccaggtga ataccggcac cgggggcggt ggaggcggtg gcggtgcaag
     3181 atccgccgcg aagggtgaag ttgcggtcaa aagtaggcca gccgtcgcgc cggcaaggcg
     3241 ccgcgagaac attcgttgca tggaatcctt ccctccgtcg tttggactta tcgctcattt
     3301 ccgtcatttg cgctaactgg tgtcatattc ataacattaa gccttcgatg aacaagctag
     3361 aatactgggt tggacggacg actcgctcat tattccaaga gatgttgcat ttttgccgtg
     3421 accaaagtct agcgttcaca gtccaatggt agaggtatgt gccttctgaa gcgcaatttc
     3481 cgccggaata tgccctagcc gtcgatgcaa ccaaatgcgc ctttagggct gtgttttttc
     3541 ggcctaatca aaccgctcgg cacacgtccc agattccttg gccgtcctct gatccgccac
     3601 agaagtgcct ctcgtgcggc caaaattgaa tgcggtcatt tgccgtcacg gtccttctcg
     3661 ctgccggaca ccctgcagac tgcggcctca ggctgtgagg aaccagccaa tcccgcggtt
     3721 attacacaga ggagcaaaga tagcctgctt ggcggcttgg ccagcgctgt tccacatttt
     3781 gcttctcctg ccgcaaatca aggccgagcc tcgacggaag agttgggagc ctcgtttatt
     3841 gctgcaggtc ttaactgggt aagccgacgg ttcgcaatgg cacatgaagc acactgctgt
     3901 cccggtgccc gcttgctgcg cccttgtctt gcgctttgtt gcgctctgag gttccccgtc
     3961 cggtaaccaa tcgggaagaa tagttcgccg cagtcctggc gcatgcaaag cccttatcga
     4021 acatctagca gatcacagtg tcccgtccga agtgcaatcg cctgggctct atcggggcaa
     4081 atcgccagga aaaagccccc gccccttgtg ccgccaccgg gccagctact ggcgggcggc
     4141 ttgactacaa ctccaggcga tgccactcgc aagcaacaag aaatcttata tccgggatcg
     4201 gaaattgaga gctttcattt ttccaggaca aggatctcaa gcagcaggta tgggtaaggc
     4261 gctggccgat gcgtttccgc aggcccgtgc aactctgagg gaggtcgacg aggcgctcgg
     4321 gcaaaatctg tccttgctca tgttcgaggg cccgcaagct gaactcacct tgacggcgaa
     4381 cgcgcagccg gcattgctcg cggtcagcct cgcagtcgtc cgtgtgctcg aagccgaggc
     4441 cggacttaac ctcgcccgcg atgcgcgctt cgtcgctggt cattctctcg gcgagtattc
     4501 tgcgctcgcc gcggcgggat ctttgagcgt tggagaagca gcaaagctgc tgcgcgtgcg
     4561 tgggcttgcc atgcaggagg ccgtgcccgt tggcgctggt gcaatggcgg cgctgatcgg
     4621 gctcgattgc gaggcggccc aggccatcgc cgagcgagct gcgatggagg cgggcgatgg
     4681 tgtgatttgc gaggtcgcca atgacaatgg gggcggccag gttgtggtgt cggggtcagt
     4741 tatcgcggtg caccgtgcag tcgaactcgc caaggaacaa ggcgcacgcc gttcaatctc
     4801 gttgcccgtg tcggcgccgt ttcattgttc cctaatgcag cctgccgcag aggccatggc
     4861 tgaggcgctc gtttctgcgg ccatcaagcc cccctgcgtt ccaattgtct cgaatgtacg
     4921 agcggctcct gtcaccaacc cggaggagat ccgtaagctt ctcattgccc aagtgaccgg
     4981 cacggtgcgc tggagggaaa gcattgtgtt catggccggc gccggaattt cttgtttcgt
     5041 tgagtgcggt gccggcaaag tcttgtctgg acttgtgaaa cgaattgcca cttcggcgac
     5101 cagtatttca attggagttc cagatgatat cggacgccta agctctgaca tccattgcgg
     5161 gagtttctaa gcgatgtttg atctaacagg caagacggcc cttgtaacag gcgcaagcgg
     5221 cgggcttggc cgcgctattt cgcgagcgct gcacgatcag ggcgcagtgg tcggattatc
     5281 gggcacacgc ggcgatgcac tcggcgcgct tgcctccgag cttgagacta atgttcatgt
     5341 tttcccatgt gatctggccg agaaatccgc gaccgaggca ctaattcccg ccgttgaagg
     5401 cgcgatggga agcatcgaca tccttgtcaa caacgcgggc ataacccggg ataatctatt
     5461 cctgcggatg acggacgaag agtgggaaac tgtacttgcg gtcaatttga ccgctgcatt
     5521 ccggctttcc cgggcatgcc tgaagggaat ggtacgtagg cggcatggcc gcatcatcgg
     5581 catcagctcg gttgttgggg tgactggcaa tcccggtcag ggcaactatt gcgccgcgaa
     5641 ggcgggcatg atcggaatgt ataagagcct cgccgccgag gtggcgaggc gcaacgtgac
     5701 ggtaaattgc gttgcgcctg gctttatcga gagtccgatg accgatgcgc taaacgacaa
     5761 acagaaatac gcaattctcg ccaccgtgcc cacggggagg ctcgggactg gcgacgacgt
     5821 cgccgctgct gttgtttacc ttgcaagcgc gcaagcctcc tatgtcaccg gccagaccct
     5881 tcatataaac ggtgggatgg caatgatttg aatagggtat gggatagttc gccatcgtcc
     5941 cgctggctct cgccaaccgc gacgaagtat gttacctacc gcacttaagc ggagtcagct
     6001 cgcgggcacc cgcatcgtgt tgacgcctgc ctattgcggc gcgttgtgag cagttattac
     6061 ataatgagta cgaggaacgt agcaccatga gcgatgtcgc cgagcgggtg aaaaagattg
     6121 tagtggagca tctcggcgtc gatgccgaca aagtcatcga cagtgccaat tttatggaag
     6181 acttaggcgc cgattcgctc gacacggtcg agctcgttat ggctttcgaa gaggaattcg
     6241 gggttgagat tcctgacgac gcagccgaaa caatcgtgac ggtcggggac gccatcaaat
     6301 tccttgagaa ggcctccgct gcttgatttc ctctgcggag gatcgacaga gcacccaatt
     6361 agaaagagac aagggtgcct tttagcggag ccatgccttc gaactgcttt ctgctccacc
     6421 gcgaaggtgg cttacgcttg cgcgttatcc gcttcgttgg ggcaaaaggc gggtggcgac
     6481 gccggcggta acaatcgcaa ggttgcttag atgcgaaggg tagtaattac gggtctaggt
     6541 ttggtatcgc cactcggtgg aggagtcgag ccaagttggg aacgcttgct atctggacaa
     6601 agcggcgccc gtcggatcga aaatttcgat gtttccgacc tggcgtgcaa gattgcaatg
     6661 cctgttccac gcggtggcga agggaatttt gaacccgacc aatggatgga accgaaagaa
     6721 cagcgcaagg tcgacgatta catcctttat gccgtggccg cggcgaccca agcgcttgcc
     6781 gacgcaggct ggaagccttc aacctatgaa gagcaaatca acactggggt ccttatcggc
     6841 tcggggattg gcggtctcgg aggaattgcc gaaaccgcaa tcgtcttgaa ggaaaaaggc
     6901 ccgcgccgcg tctcgccgtt ctttattccc ggccgcctga tcaatctcgc cggtggctat
     6961 gtttcgatcc agcatggtct caaggggccg aatcatgcgg tcgtcacagc atgctcttcg
     7021 ggcacccatg cgatcggcga cgcggcgcgt cttattgctc tcggcgacgc ggaagtcatg
     7081 gttgcgggtg gcacggaatc ggcaattaac cgcattggca tcgccgggtt tgccgcttgt
     7141 cgggcattgt caactaagtt caacgaccag cccaaacggg cctcccggcc gtatgacaag
     7201 gaccgcgacg gcttcgtgat gggcgaaggt gctggttgcg tagtcctaga ggcctatgaa
     7261 catgccatgg cgcgcggcgc caaagtttat gcagagcttg ccggctacgg catgtctgga
     7321 gacgcgtacc acattaccgc acccgacgag agcggcgatg gggcttgcag ggcgatggcg
     7381 gccgcgatca agcgggctgg tatttctccg ggtgatatcg actatatcaa tgggcatgga
     7441 acctcgacgc cgcttggcga cgaaatcgag attcgggccg tcgaaaaagt cgttggcaat
     7501 gaccgtcact ccttgtctat gtcctcgacg aagtcggcgg tcggacatct cctcggcgca
     7561 gcgggcgcgg tcgaagcaat tttttccata ttggcaataa gagatcaaat tgttccgccg
     7621 acactaaacc tcgaccagtt gtcggtcgaa actgagattg atctggtgca acatacggca
     7681 cacaagcgcg aagtcaagtt cgtgctatcg aattccttcg gctttggcgg taccaatgcc
     7741 tgtttggtct tcaaacggcc agagcgaccg ggtccagcgg tgccgcgctc ttcgacgtga
     7801 ggccagatgc gaaattcctg atccgacgaa ccagatttcg ccatattttc cgggcgggat
     7861 agatagctcc ggatgggcat agcttcggcg ccaaggcatt ttgggtgacg cgaagccact
     7921 tctcatcaga agattgtagt ttggcgaacg attatgcttg gcgccatggc tacgacacat
     7981 ggcattcgaa ttattcttct tctcgaggct atcgcaatgg aatgtgtgag ctgaagtggt
     8041 gggccatatg ccttcaaacc tcaacggaaa tgatgtgccc gacagggacg aagagtcact
     8101 ccgcgaagaa cagcagcaaa gcggccggtt cgtgggacaa agggtaacgc ctcgaagccc
     8161 aaaggaagca cttcagcccg ataccccccc accgccgccg ccaattcccc caagccgaag
     8221 gcgccccgtg ctttcggcgt tgagcggatt tctgtccttt cttctggcca tggcgattgc
     8281 cgccgtctcc ggcctcatct ggagccagca tcagctgcat gagcctggtc ccctcagcac
     8341 cagccgggtc gtgtacattg cgcccggcac cgaggttcct gacattatag ccgcgcttga
     8401 acgtgacggt gtaatcgaca gtccgttctt gctaaatgct acgctcttgg tcgaagggaa
     8461 ccgctcgaag gtcaaagctg gcgaatatct atttaaacaa aatgccagtt tacgcgatgt
     8521 gatcgacacg cttgtcagcg gcaaacaagt gctgcacgcc atcacgatac cagaggggct
     8581 aacaagcgag cagatcgtcg agcgtctcag ggaaaacgac atattgatcg gcgacgtcat
     8641 cgacatcccg aaggaaggca gcttgcttcc ggaaacttac aaagtctctc gcggcactct
     8701 tcgtgccgac gtcatcagaa agatgcagga ggaccaaagg cgtacggtgg atcagatttg
     8761 ggctcgcagg gcagccaacc tgccgctgcg ctcgccctat gaactcatca ccctagcctc
     8821 gattgtcgaa aaggagacag ggaaagccga cgagagaccg cgcgtcgcaa gcgttttcct
     8881 caaccgcctt agaaaaggaa tgcggctgca atccgatccg acgattgttt atggcctagc
     8941 cggaggaaat ggtgcgttcg gccgaggcat tacgcgatcc gaactcgaaa
//
LOCUS       DATMPU010000377         3233 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_2457989,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000377 DATMPU010000000
VERSION     DATMPU010000377.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 3233)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3233)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3233
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2457989"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..989)
                     /gene="trpB"
                     /locus_tag="VNY06_08610"
     CDS             complement(<1..989)
                     /gene="trpB"
                     /locus_tag="VNY06_08610"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008963153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HWX14885.1"
                     /translation="MALVNSFRTGPDERGHFGIFGGRFVAETLMPLILDLEKHYSAAK
                     TDPFFKAELDHLLAHYAGRPSPLYYAERLTAYVRQKAAASGRPGGAKIYFKREELNHT
                     GAHKINNVLGQILLARRMGKSRIIAETGAGQHGVATATACARFGLDCVVYMGSVDVER
                     QKPNVFRMNLLGAKVVPVESGSKTLKDAMNEALRDWVTNVENTFYCIGTVAGPHPYPA
                     MVRDFQCVIGEEAKAQMQALEGRLPDSLCACIGGGSNAIGLFHPFLDDASVKIFGVEA
                     AGYGLDKKHAASLAGGRPGVLHGNRTYLLMDADGQIEEGHSISAGLDYPGIGP"
     gene            complement(1062..1736)
                     /locus_tag="VNY06_08615"
     CDS             complement(1062..1736)
                     /locus_tag="VNY06_08615"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011470876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004640 - phosphoribosylanthranilate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HWX14886.1"
                     /translation="MSVIVKICGLATEEGLDAALAHGADMAGFVVFENSPRHVSLELA
                     AGLGKRTGNRTRKVLLTVDADDALLAAAIAALDPALLQLHGSETPKRVASIRARFGLP
                     VIKAIGIGEAADLALISQFDAVTDFLLFDTKLQSQAKIAGGTGKRFDWSLLAGLETKK
                     PWLLAGGLHAGNVGDALAQTCAPGVDVSSGVENAPGVKDGNKIADFIAAARLASRVPR
                     PFASSG"
     gene            complement(1776..2099)
                     /locus_tag="VNY06_08620"
     CDS             complement(1776..2099)
                     /locus_tag="VNY06_08620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX14887.1"
                     /translation="MKRFLKLLFLLPLAIAVLVVAEANRHPVHIYLDPLAGSSPEGTQ
                     ITVPLFAVIFVGIMIGVIFGSVVTYFEQGRHRRAARLARADAESLRAELARLSRPRPG
                     EKGRA"
     gene            complement(2256..2546)
                     /locus_tag="VNY06_08625"
     CDS             complement(2256..2546)
                     /locus_tag="VNY06_08625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011155637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HWX14888.1"
                     /translation="MIKSELVQRIASRNPHLYLRDVEKIVNAIFDEITAALSRGDRVE
                     LRGFGAFSVKHRDARVGRNPRTGAQVPVAEKTVPFFKTGKEMRERLNQRQGS"
     gene            complement(2631..>3233)
                     /locus_tag="VNY06_08630"
     CDS             complement(2631..>3233)
                     /locus_tag="VNY06_08630"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012589194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HWX14889.1"
                     /translation="SGGYIAALGTDQIVALGNALVGSIGVLIQYPNVSKMLDSIGVKM
                     EDVKSSPLKAAPNPFEPTTPEAQAALAALVGDSFSWFKSLVKERRNMTGEELQAVADG
                     RVFTGRQGIDLHLVDRLGSEREAIDWLEQEKNIPKGLKVRDWKQDRTLERLGILTLSA
                     RAAEMLGLDGLSHALDRAASAAEARTLEGLLSIWQVGSVN"
BASE COUNT          686 a          983 c          904 g          660 t
ORIGIN      
        1 tcggggccaa tcccgggata atcgagccca gccgagatcg aatggccttc ctcgatctgc
       61 ccatcggcat ccatcaacaa ataggtccgg ttgccatgca gaacgccggg cctgccgccg
      121 gcgagggaag cggcgtgttt cttgtcaaga ccatagcccg ccgcctcgac accaaaaatt
      181 ttgaccgaag catcgtcgag gaagggatga aagagcccta tcgcattcga gccgccgccg
      241 atacaggcac aaagagaatc gggcagccgg ccttcaagtg cctgcatctg cgctttcgct
      301 tcctcgccga tcacgcattg gaaatcacgc accatcgcgg gataaggatg cgggccagcg
      361 accgtcccga tgcagtaaaa agtgttttcg acattggtga cccagtcgcg cagagcctcg
      421 ttcatcgcgt ccttaagagt cttggacccg gactcgaccg gcacaacctt ggcgccgagc
      481 agattcatgc gaaacacatt gggcttttgc cgctccacat cgacggaacc catgtaaacg
      541 acgcagtcga gcccaaaacg cgcacatgcc gtggcggttg caacgccatg ctggcctgcc
      601 cccgtctcgg cgatgatccg cgacttgccc attctccggg caagcagaat ctggccgagc
      661 acattattga tcttatgcgc gccggtgtgg ttgagctcct cgcgcttaaa atagattttt
      721 gcgccgcccg gccgccccga agcggcggct ttttggcgga cataggccgt caagcgctcc
      781 gcataataga gcggactcgg tctgccggca taatgggcga gcagatgatc aagctcggcc
      841 ttgaagaaag gatcagtctt ggcggcgctg tagtgttttt cgagatcgag gatgagcggc
      901 attaaggttt cggcgacgaa cctgccgcca aaaatgccga aatggccgcg ctcatcgggg
      961 ccggttcgaa aggaattcac taacgccaac gtgttttgcc tttcttgctc aggactcgaa
     1021 caggatcggt ttatctctat caccacagcc ggccatgagt gttagccgct gctggcaaag
     1081 ggacgaggca ctcgcgaggc aagcctagct gccgcgatga aatccgcaat cttgttccca
     1141 tccttcacgc ccggagcgtt ttcgacgcca gacgagacat caacccccgg cgcgcaggtt
     1201 tgcgcgagcg catcgcccac attgcccgcg tgaagaccac ccgcaagcag ccagggtttt
     1261 ttggtctcta acccggcgag caagctccaa tcgaatcttt ttcccgtccc gcccgcgatt
     1321 ttcgcttgcg actgaagctt ggtgtcaaat agcagaaagt cagtcacggc atcaaattgc
     1381 gagataagcg cgagatcggc tgcctcaccg atcccgatcg cctttatgac cggcaggcca
     1441 aagcgggcac gaatggaagc gacacgtttt ggcgtttcgc tgccgtgcag ctgaagaagc
     1501 gccggatcga gcgcggcgat cgcagccgcg agcagggcat catcggcatc gaccgtcagc
     1561 aaaactttgc gcgtgcgatt tccggtccgc ttgcctagac ctgccgcaag ctccaaggag
     1621 acatgacgcg ggctgttttc aaaaacgaca aacccggcca tgtcggcccc gtgcgcgaga
     1681 gccgcatcga gcccttcttc cgtcgcaagg ccgcagattt tgacgatgac gctcatagag
     1741 gccctgccag catcctttag aagttgcttt gtctctcaag cccttccctt ttcgcctgga
     1801 cgcgggcggg acaggcgggc gagctccgca cgcaaagact ccgcgtcggc tcgcgccaag
     1861 cgcgcggcgc ggcgatgcct gccttgctcg aagtaagtga cgacacttcc gaaaatcact
     1921 ccgatcatga tcccgacgaa aatgacggcg aagagcggca cggtgatttg cgtgccttcg
     1981 ggcgaagaac cagccaaagg atcaagatag atatggacgg gatggcgatt ggcctcggcc
     2041 acgaccagca cggcgatcgc aagcggaagc aaaaaaagga gttttagaaa ccgcttcatt
     2101 tttatccgcc ggtgaattcg cgcctgctcg ccgcgcttct agatgatttt tggccagttg
     2161 gtaaggtctc gcgcaaacgg atagagcgaa gcgcgggaac cggcggcata gcagggccgc
     2221 gcgctttgcg cctcaacgta atggcgcact gagcgtcaac tgccctggcg ttggttcaaa
     2281 cgttcccgca tttccttgcc ggtcttgaag aagggaacgg ttttttcagc gaccggaacc
     2341 tgcgccccgg tgcgcggatt gcggccaacc cgagcgtccc ggtgcttcac cgaaaaggct
     2401 ccgaatccgc gcagttcgac ccggtctccg cgcgacaggg cggcggtaat ttcatcaaag
     2461 attgcattga cgattttttc gacgtcgcgg aggtagagat gcgggttgcg gctggcaatc
     2521 cgttgtacaa gttcggattt gatcatttgc cccgtccaac aattttttgt gccgaaaact
     2581 cgttctatga ccaaaactga cgtcgatgtt ttaccttaga ttttcctagt ctagtttacg
     2641 ctgccaactt gccaaatcga taacagccct tcaagcgtgc gcgcctcagc agcgctcgcg
     2701 gcccgatcca gcgcatggct caatccatcg agaccgagca tttcagcggc ccgcgccgac
     2761 aaggttaaaa ttcccagccg ttcgagcgtt cgatcctgct tccaatcccg cactttgagc
     2821 cctttgggaa tatttttctc ttgctccagc cagtcgatgg cttcgcgctc gctcccgaga
     2881 cgatcgacca gatgcaaatc gattccctgg cggcctgtga aaacacgtcc atcggcgacc
     2941 gcctgcagct cctcgccggt catgttcctg cgctctttta ccagcgattt gaaccaggag
     3001 aagctatcgc ccacgagtgc ggcaagcgca gcttgcgcct ctggagtcgt cggctcgaat
     3061 gggttcggcg ccgccttgag cggggaggat ttcacatcct ccattttcac cccgattgaa
     3121 tcgagcattt tggaaacatt cggatattgg ataagcacgc cgatcgaacc aaccagcgca
     3181 ttcccgagcg cgacgatctg gtccgtcccg agggccgcga tataaccacc gga
//
LOCUS       DATMPU010000378         3295 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_4631510,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000378 DATMPU010000000
VERSION     DATMPU010000378.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 3295)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3295)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3295
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4631510"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..482)
                     /locus_tag="VNY06_08635"
     CDS             complement(<1..482)
                     /locus_tag="VNY06_08635"
                     /inference="COORDINATES: protein motif:HMM:NF015425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-3B synthase"
                     /protein_id="HWX14890.1"
                     /translation="MIDAAQMRKGWCPGARRPMRAKDGLLVRLKISCATLSAKTLRGI
                     AQAGQRYGNGQFDLTSRASLQIRGVREDKLPLLIKVLDRFGLIDRNAPAEAVRNVLVS
                     PLAGLDGRCSAHVAAKALEATLAVNADLEALPAKFGFLIDDGSAPSLAGIPADIRFGW
                     "
     gene            complement(479..>3295)
                     /gene="cobN"
                     /locus_tag="VNY06_08640"
     CDS             complement(479..>3295)
                     /gene="cobN"
                     /locus_tag="VNY06_08640"
                     /EC_number="6.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012592244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobaltochelatase subunit CobN"
                     /protein_id="HWX14891.1"
                     /translation="CRKRAWPRGQALILFYRSFMVAEDTEAILALADALAAQEISVTT
                     IFVSSLKDSDAIAFLRAELAEVKPDVIVNTTAFSARLTNESVLDTSGLAVLQAIFSGA
                     TEAQWKENPRGLSAADLAMNIVLPELDGRIITRAIACKQEGALRADLEFAPRSHVPLH
                     SRVKFVADLAAGFVKLRKTPRARRKIACVLPDYPGRHGRTGYAVGLDTAKSVTSIAAT
                     LREAGYSISMLPDPNALMRRLEEGDTAERFSLSDYSFALKQMPPRFVESLVAQWGAPD
                     TEAPGGAFVIPIVRAENLIIALQPNRGLRANRKVDYHDTALPPCHSYVAFYIWLRQKE
                     QIDALIHCGTHGTLEWLPGKAVALDEDCAPEAVLGPLPVIYPFIVNNPGEAAQAKRRI
                     CALTIGHMTPPLELAASHGAALEIESLFDEYAIAEPLDLKRARLLAQAILAQAKETGL
                     LQESGLKNIADPEAALRHLDAWLCDLKEMRITNGLHVFGQSPQAEFRDATLAALTTAQ
                     DTDDSSPSLSSLIDHCGEAERASLVAALDGSFVAPGPGGAPSRGRIDVLPTGRNLYGI
                     DPRAVPTRTAWEIGQRAAQEVLNRHVQDHGEWPKRIVMDLWASATMRTGGDDLAQALA
                     LMGVTPLWDNASSRVKGFEIVPPARLAHPRVDVTLRISGLFRDVFPAQIAFFDQAVQA
                     LSELDEEDDVNSLAAARRLGSKDLSRIFGAAPGAYGIGLMQAIDQDPAADRDALGARY
                     LAAASHAYRGLQAEGIATMDFTDRVANADAFVHVQDQDEQDILDSSEAVDHEGGFAAA
                     AQMLGNDAPAYHIETTRPGAIKVRSLAQEIARVVRARAANPRWIAGQMRHGHRGAAEI
                     AQTIDNLYALAVLSDAVSTQHFELLFEATIGTPLVRDFLIDTNPRAAQAIAKRFEDAL
                     ARGFWQTRRNSPHACLAKMRGASA"
BASE COUNT          694 a          964 c          972 g          665 t
ORIGIN      
        1 tcccaaccga atcggatgtc ggccggaata ccggccaaag aaggcgcgct tccatcatca
       61 atcaagaaac cgaatttcgc cggcagtgcc tcaagatcag cgttcacggc aagcgtcgct
      121 tcgagggctt ttgcagccac atgcgcagaa catcgtccgt caaggcccgc cagcgggctc
      181 accagaacat tgcgaacggc ctctgccggc gcgtttctat caatgaggcc aaatctgtcg
      241 agcactttga taaggagcgg caacttgtct tcgcgcacgc cgcgaatttg cagactggcg
      301 cgagacgtaa ggtcaaattg cccattgccg taacgttgtc ctgcttgcgc gatcccgcgc
      361 agggttttag cggagagcgt ggcgcaactg attttgaggc gcacgagaag cccgtccttg
      421 gcccgcatcg gccgccttgc gcctggacac cagcctttac gcatttgagc cgcgtcgatc
      481 atgcgcttgc tccccgcatc ttggcgaggc aggcgtgggg cgagttgcgg cgggtctgcc
      541 aaaagccccg ggcgagcgca tcttcaaagc gcttggcgat cgcctgcgcg gccctcggat
      601 tagtatcgat aagaaaatcc cgcaccaaag gcgtgccaat ggtcgcctcg aacaaaagct
      661 caaaatgctg ggtcgatact gcgtcgctta gcaccgcaag ggcataaaga ttgtcgatcg
      721 tctgggcgat ttcggcggcg ccgcgatgcc cgtggcgcat ctggcctgca atccagcgcg
      781 ggtttgccgc ccgcgcacgc acgacccggg caatttcctg cgccaagctg cgcactttta
      841 tcgcgccagg acgcgtcgtc tcgatatgat aggcgggcgc atcattgccg agcatttgtg
      901 cggcagcagc aaagccgcct tcatgatcaa cggcttcgct cgaatcgaga atgtcctgct
      961 cgtcttgatc ttggacatga acaaaagcgt cggcattggc gacgcgatca gtgaagtcca
     1021 ttgttgcgat gccttcggct tggaggccgc gataggcatg cgaagccgcc gcaagatagc
     1081 gcgcgccaag cgcgtcgcga tcagccgcag ggtcttgatc tattgcctgc ataagaccaa
     1141 taccataggc gccgggagct gcgccaaaaa tccgcgatag atctttggat ccgagccggc
     1201 gcgctgccgc aagcgaattt acatcatctt cttcgtcgag ctcgctcaac gcctgcacgg
     1261 cttgatcgaa aaatgcaatc tgagctggga aaacatcgcg gaacaggcca gagatccgca
     1321 aggtgacatc gacgcgcggg tgcgcaagac gcgcgggagg aacgatctca aagcccttca
     1381 cacgagagga cgcattgtcc caaagcggcg taacgcccat aagcgcgagg gcttgggcga
     1441 gatcgtcgcc acccgtgcgc atggtggcgc tcgcccaaag atccatgaca attcgttttg
     1501 gccattcgcc atggtcctga acatggcggt ttagcacttc ttgtgctgcg cgctggccaa
     1561 tctcccaagc cgttcgcgtc ggcacggccc gcggatcgat gccgtagaga ttgcgccccg
     1621 tcggcaatac gtcgatgcgg ccgcgcgacg gcgcgccgcc tggacccggc gcaacaaagc
     1681 ttccatcaag tgcggcaaca agcgaagctc gttcggcttc gccgcaatga tcaatgaggc
     1741 tactaaggga cggagaagaa tcgtcggtat cctgcgctgt tgtcagtgcg gcaagtgtcg
     1801 cgtctctgaa ctctgcttgc ggtgattgcc cgaaaacatg caggccattg gtgatccgca
     1861 tctccttaag atcgcaaagc caggcatcga gatgccgcag cgcggcctcg ggatcggcga
     1921 tatttttgag cccgctctcc tgcagcagcc ctgtctcttt ggcctgggcc aaaattgctt
     1981 gcgccaatag ccttgcgcgc ttgagatcaa gaggttcggc aatcgcatat tcatcaaaca
     2041 aggattcgat ttcgagcgcc gcgccatgac ttgcagcaag ctctaggggt ggagtcatat
     2101 ggccgatcgt gagcgcgcaa atgcgccgtt tcgcttgcgc cgcctcaccc ggattattga
     2161 cgatgaaagg ataaattaca gggagaggcc cgagcactgc ttctggagcg cagtcctcat
     2221 caagcgccac ggccttgcca ggcagccatt caagcgtgcc atgcgtaccg caatggatca
     2281 gtgcgtcaat ctgctccttc tggcgcagcc atatataaaa ggccacatag gaatggcagg
     2341 gcggcaacgc cgtatcatga tagtccacct tgcgatttgc tctgaggccg cggttgggct
     2401 gcagggcaat aatcaaattt tccgcacgca caatcggaat gacaaatgcg cctcccggcg
     2461 cctctgtgtc cggagcgccc cattgcgcga caagtgattc cacgaaccta gggggcattt
     2521 gtttgagggc gaagctgtaa tccgaaagcg aaaaacgttc tgccgtatcc ccctcttcca
     2581 gccttcgcat caaagcgttg gggtccggca gcattgagat agaatatccg gcttcgcgta
     2641 gcgtggctgc aattgaggtg acgctctttg ccgtgtcgag gccgaccgca tagccggttc
     2701 ggccgtgccg tcccggataa tctggcagga cacaagcgat tttgcgccgc gcgcgtggcg
     2761 tcttgcgcaa tttgacaaaa cccgcggcaa gatcagcgac gaatttcacg cgcgagtgca
     2821 ggggtacgtg actgcggggc gcaaattcaa gatccgcgcg aagcgcgcct tcctgcttgc
     2881 aggcgatggc gcgagtaatg atccgcccgt cgagttctgg caaaacaata ttcatggcca
     2941 gatcggccgc acttagtccg cgcgggtttt ccttccattg cgcttctgtt gcgccggaga
     3001 agatcgcttg cagcactgcc aaaccagagg tgtcgaggac actctcattt gtgaggcggg
     3061 ctgagaacgc ggtcgtattg acaatcacat cgggtttcac ctcggcgagt tctgcgcgca
     3121 gaaaggcgat tgcgtcagag tccttaaggc ttgagacgaa gattgtggtt acgctaattt
     3181 cttgcgcagc gagcgcgtca gcgagcgcca aaattgcctc ggtatcttcc gcgaccataa
     3241 aggatcggta gaaaaggatg agtgcttggc ctctgggcca agcgcgcttt cggca
//
LOCUS       DATMPU010000379         3015 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_4309271,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000379 DATMPU010000000
VERSION     DATMPU010000379.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 3015)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3015)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3015
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4309271"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            18..248
                     /locus_tag="VNY06_08645"
     CDS             18..248
                     /locus_tag="VNY06_08645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011926608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX14892.1"
                     /translation="MTETPAMFEPSKPITKVERDARKAFRQVDAKKAMTEHEIVQKAF
                     HANRERLKAERLAREAGTQVAAKKAESKKKSK"
     gene            complement(473..982)
                     /locus_tag="VNY06_08650"
     CDS             complement(473..982)
                     /locus_tag="VNY06_08650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX14893.1"
                     /translation="MNLPNPVPEAAVAFALLLESACQEFETLQGLLAGDLQVVAARPG
                     AERSAIKSAGAIRMALAKSFLFNANRANRVCLKNKASLAIDRDERERFLSATKPLVDV
                     RDVNEHGYDGDRRSEKQRPSMHEQEGGFLDETSLIVASPIKILMGPLNLYDIYNAVAR
                     MRAIAGRFG"
     gene            complement(979..1344)
                     /locus_tag="VNY06_08655"
     CDS             complement(979..1344)
                     /locus_tag="VNY06_08655"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX14894.1"
                     /translation="MSYREEGRVFRHPDRRVIFVGDFIDRGPQQMDVLNIAKGMCEAG
                     TVLAVIGDHEFNALGWATPDGNGGFLRPHSLQNHGQHKEFLRQVGEGTKVPASPDWLH
                     EIKFDGYRLHGPLAIWSKA"
     gene            complement(1764..2021)
                     /locus_tag="VNY06_08660"
     CDS             complement(1764..2021)
                     /locus_tag="VNY06_08660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX14895.1"
                     /translation="MGRNVLRASFAELTDKVAESWGHWLFAKEAPQTFSYLSANGLIV
                     LSVNVQVTHLSAHAPAVARRAGLSPEKVRPQEGDQRGTKPP"
     gene            2297..2455
                     /locus_tag="VNY06_08665"
     CDS             2297..2455
                     /locus_tag="VNY06_08665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX14896.1"
                     /translation="MAAKAPRKIEKMALGEMRASGVRGLLIYCADYRCSHSVEMSGDR
                     WADDVRLT"
     gene            complement(2512..2706)
                     /locus_tag="VNY06_08670"
     CDS             complement(2512..2706)
                     /locus_tag="VNY06_08670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018321076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX14897.1"
                     /translation="MKVTKPKKNGKAVRREWTKADVKELKAHSKARTPVTKISKETKR
                     TIGALRQKALHLGIGLGHQR"
     gene            complement(2809..>3015)
                     /locus_tag="VNY06_08675"
     CDS             complement(2809..>3015)
                     /locus_tag="VNY06_08675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX14898.1"
                     /translation="AAASEVAAMPAGTHEVLDQTREAVERRARILLAQLQELGDDPVK
                     AVNVSKVPLRSYGKSSKTKALGWE"
BASE COUNT          677 a          848 c          827 g          663 t
ORIGIN      
        1 cggacgcaag gtgagaaatg acagagacac cggccatgtt cgaaccatcc aagccaatca
       61 ccaaagtaga gcgcgatgcc cgtaaggcat ttcgccaagt cgatgccaaa aaggcaatga
      121 cggagcacga gattgtgcag aaggcattcc atgcaaaccg ggagcggctt aaggccgagc
      181 ggctggcgcg agaggctggg acgcaggtcg cagcgaagaa agccgagtcc aagaagaaga
      241 gcaaatagcg ccacgatggc cggcctcagg gtgtggaatt ggacgggccg atccgccgcg
      301 tagtcgagcg cgagctgaag gcgaagaaat gggcgagctt gaggcaacgc cagaacttcc
      361 ggatggaatg ccgatccgtg aggtccgatt cccgacgcgc ataaagaacg cgaccaaaca
      421 aactccggtt caagatcggg tggccgaaca tcatccagcc atcggtcgcc gcttagccaa
      481 agcgccccgc gatggctcgc atccgtgcaa cagcgttgta gatatcgtaa aggttcagcg
      541 gtcccataag aatcttgatc gggctggcga caatgagact ggtttcatca aggaagccgc
      601 cttcctgctc gtgcatagat ggtcgctgct tttcggagcg ccgatcaccg tcatagccgt
      661 gttcattcac gtctcggacg tcaaccaaag gcttcgtcgc gcttagaaat cgctcacgct
      721 cgtcgcgatc gatagccagc gaagccttgt ttttgaggca cactcgattg gcgcggttcg
      781 cattgaacag gaatgattta gcgagtgcca ttcggattgc tccggcgctc ttgattgcgc
      841 ttcgctccgc gccaggacgc gctgccacca cttgcaaatc gccagcaagc aacccttgca
      901 gcgtctcaaa ttcctggcag gcactttcca gcaaaagggc gaacgccacg gcagcctccg
      961 gtactggatt gggcaggttc atgctttgct ccaaatggcg agtggcccat gcaggcggta
     1021 cccatcgaac ttgatctcgt gaagccagtc ggggctggcg ggaaccttgg ttccctcacc
     1081 tacctgacgc aaaaactcct tgtgctgtcc atgattctgt aaactgtggg gccgaagaaa
     1141 gccgccattt ccatccggcg tcgcccaacc taatgcgttg aactcatgat cgccgataac
     1201 tgcaagtaca gttcctgctt cgcacatacc tttagcaatg ttcagcacgt ccatctgttg
     1261 ggggccgcga tcaatgaaat cccccacaaa aatgacgcgg cggtccggat gacggaagac
     1321 acgcccttcc tcgcgatagc tcatatcgct taaaagattt ctgaaagctt tgcgtgccca
     1381 tggatgtcgc cgatgacatc gaatttattt agactcattt actagcttcc gcatctgaac
     1441 ccaactccgc cagaaacgtt cgagcaaacc gatagtcgtc ttcatattcg gcaaacccct
     1501 taggctatcc agtgccgatt gatgaaatgg cagatgcgga atgttaacgg ctggcatcac
     1561 gaacggcgcg agcgttggcc gccatgcttt tgaaccacag gttaacgatt ggctaatctc
     1621 tcgggaccta gtcgtataat atcagtacga atctttattc cgacattcag aatctaaaat
     1681 ccgccactag cgcgtcgtaa tatcgctccg accgttcaac acaatgtgag aagcgttcga
     1741 tccgtcgatc ggcgtatgcg gcctcaaggg ggtttggtac cgcgctggtc cccctcttga
     1801 ggccgtactt tttcgggtga caatccggcg cggcgagcaa cggccggagc gtgggcgctc
     1861 aaatgagtca cttgaacatt gaccgaaagc acaatgaggc cattcgccga gagataggag
     1921 aacgtctgcg gggcttcctt cgcaaacagc cagtgccccc acgactccgc aaccttgtct
     1981 gtcaattcgg cgaagctcgc tcgcaggaca tttctaccca actcagtttc cgcatagctc
     2041 gactgcgtga gggattgata agaggcgctg gcccttgtcg ggctgatcgt aaagacaata
     2101 ccgcgagcta gacggcgtgc attggcaatg caagtactca gaagcgcgag cagcccgcaa
     2161 gtttcagcga ttggctgtag gggcggcttg taaccgagta gcccctaaat gactcagaaa
     2221 tggccggagg cgcgttttag tctgtttccg cgtccctacc gtttcgaggt gcgaaaaacg
     2281 aatccaggac tcgctaatgg ccgccaaggc accaaggaag atcgaaaaga tggcgttagg
     2341 cgagatgcgc gcctctggtg ttcgcgggtt gctgatttat tgcgccgatt accggtgcag
     2401 ccatagcgtc gaaatgagcg gcgaccgatg ggccgatgat gtccggctaa cttaaattgc
     2461 acggcgcggc acttttcgtc tttctcagtt cagccgcgcg gtgcttagcg tttaccgctg
     2521 atgcccgagt ccaatcccga gatgtagcgc cttctggcgc agcgcaccaa tcgtgcgctt
     2581 tgtctcctta gatattttgg tgaccggtgt tctcgccttc gaatgtgcct tgagttcctt
     2641 gacgtcagcc ttcgtccatt cgcgacgaac tgctttgccg ttcttctttg gcttcgtaac
     2701 tttcaacttg ttagctcctt attttgatgg ggcccgcgta ttaacatatc gcacggcaga
     2761 gacaaccaaa caaaagctaa aaatcgtcgc ctagggactt tcttcgcgtc attcccatcc
     2821 gagggccttc gtcttgctgg actttccgta tgaccgcagt gggacctttg agacattgac
     2881 tgctttgacc ggatcgtctc ccaattcttg cagttgtgcc aggagtatac gagcccggcg
     2941 ttcgacagcc tctcgtgtct ggtcaagaac ctcgtgcgtg ccggcgggca tggcggctac
     3001 ctcagacgcg gccgc
//
LOCUS       DATMPU010000380         3077 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_1845733,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000380 DATMPU010000000
VERSION     DATMPU010000380.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 3077)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3077)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3077
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1845733"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            289..483
                     /locus_tag="VNY06_08680"
                     /pseudo
     CDS             289..483
                     /locus_tag="VNY06_08680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012092488.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(652..1185)
                     /locus_tag="VNY06_08685"
     CDS             complement(652..1185)
                     /locus_tag="VNY06_08685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018688920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HWX14899.1"
                     /translation="MTVQKRSAPYLPEHARSAKSPCEGTAMDLADLIEPDRVVFAARA
                     SNKQQLLQDLASRAATLLNLDGRVIFDALQAREELGSTGLGSGFALPHARVEGLDRLF
                     GMFTRLNRPIHFDSIDGKPVDLVFLLLIPPSAGSEHLCALAAVSRLLRDQEFAASLRK
                     AGSATALCKLLCSPQHS"
     gene            complement(1182..1883)
                     /locus_tag="VNY06_08690"
     CDS             complement(1182..1883)
                     /locus_tag="VNY06_08690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020174708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HWX14900.1"
                     /translation="MTPRGGPLRVLVVDDEPAILRFLHASLESQGYIVWTAADARTTL
                     DMVCKRTADLVVLDLGLPDMDGLDVIRQIRNGGETLPIIILSSRENESAKVTALDLGA
                     DDYVTKPFGIDELLARIRTAQRHRLQQEGERPLFHAGDLSIDLVRRIVTVRGSEVKLS
                     PREYEVLRLMAAHAGKVMTHRFMLREIWGGDADVQYLRIYIRALRQKIEADPERPTHI
                     LTETGVGYRLREPDQ"
     gene            complement(1880..>3077)
                     /locus_tag="VNY06_08695"
     CDS             complement(1880..>3077)
                     /locus_tag="VNY06_08695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015887346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWX14901.1"
                     /translation="AVAARERTRITEELYLFSRKLAGVATLDDLLWTTVHQIALMLKV
                     RVVVLLPEDGGLVVKAGFPPEDMLDEADLAAARWCFERIQATGRGADTLPGAKRRFMP
                     LRTGRGAVGVVGIDSDKPGLLLTPEESRLLDALSDQAALAIDRVNLAQDIDRARLAAE
                     TDRLRAALLTSISHDLRTPLASILGSASSLVTHGQEFDATVQASLMRTILEEAERLNR
                     FIGNLLDMTRLESGPLTPQTGPADLSDIVGATLQRADKILKSHPVAIDLESGLPLLDL
                     DMVLMEQVLFNLFDNAAKYAPPGSCVMVMARLEGGSVAIRVLDEGEGIPEADLDRIFD
                     KFYRVRRSDRQRAGTGLGLAICRGFVEAMGGSITAANRIGRPGAVFTIRLPVPVTPRL
                     SAEALS"
BASE COUNT          666 a          894 c          905 g          612 t
ORIGIN      
        1 tgattttgcg gtcgcggtaa agctatagct gaatgatcac ggccggaaag tgtaatcggc
       61 gttgatgatt ttgatcatcg gcgaggcggc ttgatgttac ccgcgccttg gctttcgatc
      121 gccgtcccgg cgctcttgcg cttttccccc cgcacctgct cgtcgaagcg cagacgcagg
      181 agctccattt tcatcttctc aatctccgca aacttcgccg atggcggcta ttaaggaccg
      241 caattttgtg agacccagaa gaaagccctg ccgtatcttg cgacggtgat cgtgaaaaga
      301 tcagatggtg tgaacgggtt cgaggtcttg ccgaagggtt gggttatcga accaaccttc
      361 gcgtggcttg gccgctgtct acggctgccg aaagacttcg aaaacctatc cctgcatgct
      421 ttgccgttac tccgtctcgc cgcgatccgc ctcatgctac gaaagcttcg ttttcacaaa
      481 tgaactttcc ggactggctc taagagcgat acggccgact agctctagcg ctttagagct
      541 tttcggaagc ggtaaacaac agctaaaaaa aaagatcatg cgtgaaacaa acggattgag
      601 aacatgatcc gcgaaagtgg tacctagctt caaatttaaa gcgctgactt gtcatgaatg
      661 ttgcggactg cacagaagtt tgcatagcgc agtggcgctg cccgccttgc gaagactcgc
      721 tgcgaactct tggtcacgca gaagccgtga aaccgcggca agcgcgcaaa ggtgttcgct
      781 gccagcgctt ggggggatga gcagaagaaa cacgagatcg accggcttgc catcgatcga
      841 gtcgaaatgg atcggacggt tcagacgcgt gaacatgccg aaaagccggt cgagcccttc
      901 gacgcgggca tgcgggagcg cgaaaccact gccgaggcca gtggaaccca gctcctcgcg
      961 tgcctgtagg gcatcgaaga tcacccgccc atcgaggttc agcagggtgg cggcgcgcga
     1021 cgcaagatcc tggagcaatt gttgtttgtt gctcgcgcgg gcggcaaaaa ccacccggtc
     1081 cggttcgata aggtcggcga gatccatggc tgtaccttcg catgggcttt tagctgatcg
     1141 cgcatgttcc ggcaaatagg gagctgaccg cttttgaaca gtcattggtc cggctcacga
     1201 agacgatagc cgacgcctgt ttcggtgaga atatgggtag gccgctctgg gtccgcctcg
     1261 atcttctggc gcagggcacg aatgtagatt cgcagatatt gtacgtcggc gtcaccgccc
     1321 caaatttccc gcagcataaa acggtgggtc ataacttttc cggcatgtgc cgccatgagg
     1381 cgcagaacct cgtattcgcg gggcgaaagt ttgacttcgc tgccccgcac ggtgacgatt
     1441 cgacgcacaa gatcgatgct gagatcgcca gcatggaaca aaggcctttc accttcctgc
     1501 tgcaagcgat gccgctgcgc tgtgcggatg cgcgccagga gctcatcgat accgaaaggc
     1561 ttcgttacgt aatcgtcagc gccgagatcg agcgctgtga ccttggcgct ttcgttctca
     1621 cggctcgaca ggataatgat cggcaatgtt tcgccgccat ttctgatttg tctgatgaca
     1681 tcgaggccat ccatgtccgg cagaccgaga tcgaggacga cgagatcggc ggtgcgtttg
     1741 cagaccatgt cgagcgtggt gcgggcgtcg gcggcggtcc agacaatata gccctggctc
     1801 tccaggcttg cgtgcaaaaa gcgaagaatt gccggctcgt catcaacgac gagaacacgg
     1861 agcggacccc cgcgtggcgt cacgagaggg cctcggccga gagcctaggc gtaactggca
     1921 ccggcagtct gatggtgaaa accgcgccag gccggccaat ccggttagca gccgtgatcg
     1981 agcctcccat ggcctcaaca aagccgcggc aaatggcaag gccgagccct gtgccggcac
     2041 gctgccgatc cgagcgacga acccgataga acttgtcaaa aatccgatcg agatcggcct
     2101 caggaatacc ctcgccttca tcaagcaccc ggatggcgac ggaaccaccc tcaagtctcg
     2161 ccatgaccat gacgcacgac cctggcggcg catatttcgc cgcattgtca aagagattga
     2221 acagcacctg ttccatcaac accatgtcga gatcgagcaa tggcaaaccg ctttcgagat
     2281 cgattgcgac cggatggctt ttcaggatct tatccgccct ttgcaacgtc gcgccgacga
     2341 tgtcggagag atccgcaggt ccggtttgcg gcgtgagcgg tccggattct agcctcgtca
     2401 tatcgaggag attaccgata aagcggttga gccgctccgc ctcttccaaa atcgtgcgca
     2461 tgaggctcgc ttgaactgtt gcatcgaact cttgtccatg cgtgacgagg ctgctcgccg
     2521 agccgagaat cgaagcaagg ggggtgcgca gatcatgcga aatggacgta agcaacgccg
     2581 cgcgcaggcg atcggtctcc gccgccagac gggcgcggtc gatgtcttgg gcaagattta
     2641 ctctgtcgat cgcaagagcc gcctgatccg acaaggcgtc caaaaggcgg gattcctcag
     2701 gtgtcaaaag gagccccggc ttgtcgctat caatcccgac aacgcccacg gcaccgcgcc
     2761 cggttcgtag aggcatgaag cggcgcttgg cgcccggcag cgtatcggcg ccgcgcccgg
     2821 ttgcctgaat tcgttcaaag caccagcggg ccgcggccag atctgcctcg tcgagcatgt
     2881 cctcgggtgg aaacccggcc ttgacaacaa gaccgccatc ctccggtaaa agcacgacga
     2941 cacgaacttt cagcatcaag gcgatctgat gcactgttgt ccagagcaga tcgtcgagcg
     3001 ttgcaacgcc ggcgagcttg cggctgaaaa gatagagttc ctcggtgatc cgcgtccgct
     3061 cccgcgcggc gaccgcc
//
LOCUS       DATMPU010000381         4351 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_4639405,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000381 DATMPU010000000
VERSION     DATMPU010000381.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 4351)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4351)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4351
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4639405"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..416
                     /locus_tag="VNY06_08700"
     CDS             <1..416
                     /locus_tag="VNY06_08700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX14902.1"
                     /translation="NLVESIIARKDLPFDAQWMTETFERFYNRFGAITYAFNNILLEP
                     ITNAGKMLLITQYGSDGNSRGSTGQQRIADAFIENFNDANVLTSAFLEIHKAQAVIRE
                     KTGLSWPIAVARGALGVAKGQLRQKLGREPGHPVV"
     gene            complement(578..931)
                     /locus_tag="VNY06_08705"
     CDS             complement(578..931)
                     /locus_tag="VNY06_08705"
                     /inference="COORDINATES: protein motif:HMM:NF024584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPASM domain-containing protein"
                     /protein_id="HWX14903.1"
                     /translation="MPKNLSYSCEPDTHAALVTHDPNGLRPVWLKRGDSSSRWSLCRR
                     PWTVMYITANGRALPCCIAPFSQRSYENYTLGDATQQSLREIWNGLAYQAFRRALQSD
                     KPPATCANCGLHWSL"
     gene            complement(1050..1235)
                     /gene="nrtS"
                     /locus_tag="VNY06_08710"
     CDS             complement(1050..1235)
                     /gene="nrtS"
                     /locus_tag="VNY06_08710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012043487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter NrtS"
                     /protein_id="HWX14904.1"
                     /translation="MPKRSFFLALIVGTVLNLINQGEILFGGGKLNLTKIFLTYAVPY
                     CVATYGAVSYRLRVERK"
     gene            complement(1332..1952)
                     /locus_tag="VNY06_08715"
     CDS             complement(1332..1952)
                     /locus_tag="VNY06_08715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013947896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HWX14905.1"
                     /translation="MNIRHTEVRPRERLINAAYDLFSKDGVSQVGIDTILAKSDCAKA
                     SLYSNFKTKVELAIAFLHKREAVWTRGWLESEIKRRAKTPEGRLLAIFDVFDGWFHKK
                     SFEGCSFINVLLESKEGSPVRQAAAIHLAKIRAIVRGLAEEANLQEPEKFAQVWHMLM
                     KGSIVSAGEGNRNAARDAKYAAQLILKGWKRTREADKPRAKRGAGR"
     gene            2221..3390
                     /locus_tag="VNY06_08720"
     CDS             2221..3390
                     /locus_tag="VNY06_08720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX14906.1"
                     /translation="MDFNPRTATGIPTEPVGSIPRPLKLQQAYAAYDAKKITKAQLEE
                     EQNAAVADTIKRFEATGSPIITDGEQRVSSFATYPIADTLAGTGLAPHLAPGGQYFAI
                     FADGHNRQLPKITGGPFHYNFYAGDLVKQAVKLTSKPLHQAVIAPSMMYLLYPLDGEV
                     AGYPRKKFEEDIVNECEKDIRSCFASGAAHVVMDFTEGRLAPRNDPRNPWTTRHLLPF
                     FIELDNRVFDRFSAEERKNIGVHVCPGGDVDSVHSADVPYEELLKHLFKLNAGFFQME
                     MAGERDRESAYQLVGKYLRDDANGIPQMAYIGCIVTQSPRAESAQEVCDQLVAAAKYI
                     DKQRLGSTDDCGFSPFSIDTKPNHGSLDYARDIAFQKIANRVAGTKMAAEKLGVH"
     gene            complement(3893..4117)
                     /locus_tag="VNY06_08725"
     CDS             complement(3893..4117)
                     /locus_tag="VNY06_08725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX14907.1"
                     /translation="MPLSKVDRQQSARAQFKQHSAEHSGADKQQTDGPDRVLVNHIGV
                     HDEAHDVAYLFRLTGGMDGSYRLPCAARSR"
BASE COUNT          934 a         1275 c         1199 g          943 t
ORIGIN      
        1 gtaaccttgt agaatcgatt atcgcgcgaa aggatctgcc tttcgatgcg cagtggatga
       61 ccgaaacttt cgagcgtttt tacaatcgct ttggtgctat tacctacgcc ttcaataaca
      121 ttcttctcga gccgattacc aacgctggca agatgctcct gatcactcag tacggtagcg
      181 atggaaattc gcgcggttcg accggccagc aacgcatcgc tgacgccttc atcgaaaact
      241 tcaacgatgc caacgtgctg acatcagctt tcctcgagat tcataaagcg caagccgtga
      301 tccgagaaaa aaccgggtta agctggccga ttgccgtagc ccgcggcgca ctcggcgttg
      361 ccaaaggaca gctgcgccaa aagctcgggc gcgagcccgg tcaccccgta gtgtgaatga
      421 tgccatggct tacccagata tcgcgtacaa ggccatggct acgtatgcca acgacgattt
      481 cgaaccgatg gcggtggcag catgacggcc gagactcccg ccaacgctgt tccagcgtcg
      541 agtcagaata accgtctaag cggatcagcc cgccaagcta caggctccag tgcaatccgc
      601 aatttgcgca cgtagcaggc ggcttgtcag actggagcgc gcgacggaat gcttgataag
      661 ctaggccgtt ccaaatttcc ctcaagctct gctgggtagc atcgccgagc gtgtaattct
      721 cgtagctccg ctgcgagaat ggcgcgatgc agcatggcag tgcccggcca ttggctgtga
      781 tgtacatgac ggtccacggc cggcggcaca gcgaccatcg ggagctactg tcgccgcgct
      841 tcagccaaac aggtcgaagt ccgttaggat cgtgtgtcac gagcgccgcg tgagtgtctg
      901 gttcgcaact atacgagaga ttcttaggca tgagactaca agcctaaatc acgcggcgat
      961 aaaagctatg gtcaaagcac tcccaaaaga ggcctttcca ttgacgcaag tccaacaagc
     1021 ttatttacgc cagcattcct ttgacgcagt cacttgcgct cgactcttaa ccgataggaa
     1081 acggctccgt aggtggcaac gcaatacggt actgcatagg tcaggaagat ctttgtgaga
     1141 ttgagcttgc ctccgccgaa taggatctcg ccttggttta taaggttcag caccgtaccg
     1201 acgattaagg caagaaagaa agagcgcttc ggcaccccat ccgatacgca acagcggcaa
     1261 atctctaata gccgcaaatg gatcaccaga acttatgagc gctcatcgag aaggcctaag
     1321 aaagagctct gttaccggcc ggctccgcgc ttagcgcgtg gcttgtcagc ttctcgcgtc
     1381 cgtttccagc ctttgaggat tagttgtgcc gcgtacttgg cgtcgcgagc agcatttcgg
     1441 ttcccttcgc ctgcagaaac gatcgatccc ttcatcaaca tatgccaaac ctgcgcgaat
     1501 ttttcgggtt cctgcaggtt tgcttcctct gcgaggccgc gcacgatagc gcggatcttc
     1561 gcaagatgga tggcggcggc ctgacggacc gggctacctt ccttcgactc caacagaaca
     1621 ttgatgaagg agcagccttc gaacgacttc ttgtgaaacc acccatcaaa gacatcgaag
     1681 atcgctaaaa gccggccctc cggcgttttc gcgcggcgtt tgatctccga ctcaagccag
     1741 ccgcgggtcc agactgcctc gcgcttatga agaaatgcga tcgcaagctc aaccttagtc
     1801 ttgaagttgc tgtaaagact cgccttggcg caatcggact tggcgaggat ggtatcgatg
     1861 ccgacctggc tgacaccatc ctttgaaaat aggtcatagg ctgcgttgat aagccgctcg
     1921 cgcggcctga cctctgtgtg ccggatattc acatccgctc ccttgctgta gctcgaagcg
     1981 cgtctaagtc tctcgctgca ttcctaacat ttgatcagca ggcgtggcaa agtccgattc
     2041 tgaagtttat gcaactggtt aagcctccaa agagcgaacg attcccggat tgtcactggc
     2101 ggaaccgctt gacctccttg ccatttagca gtccgatata gacagacggg tctgtcccgt
     2161 ctagccccga ccagtcagtc gtcggaaagc gcgataaacc aacacgatgg gaggttaacg
     2221 atggacttca atccacggac tgccacgggt attccgaccg agccggtggg ttcgattccg
     2281 cggccgctca aactgcaaca ggcctatgcc gcgtacgacg ccaagaagat caccaaggct
     2341 cagctggaag aggagcaaaa cgccgccgtc gcggatacta tcaagcgctt cgaggcgacc
     2401 ggatcaccta tcatcaccga tggcgagcag cgcgtctcga gcttcgccac ctatccaatt
     2461 gcggacacgc tcgccggaac tggactggcg ccccacctgg cgccaggagg gcaatatttt
     2521 gcgatctttg ctgacggcca taatcgccag ttgcccaaga tcactggcgg cccgtttcac
     2581 tacaatttct acgcgggcga tctggtcaag caggcggtca agctgaccag caagccgctc
     2641 catcaggcgg tgatcgcgcc gtcgatgatg tatctccttt acccactcga cggcgaagtg
     2701 gcggggtatc cgcgcaagaa gttcgaggaa gacatcgtca acgaatgtga gaaggacata
     2761 cgctcgtgtt tcgcgtccgg cgccgcacac gtggttatgg acttcacgga agggcgcttg
     2821 gcgccgcgca acgatccgcg caacccatgg accacccgcc accttctgcc gttctttata
     2881 gaactcgaca atcgagtctt cgatcgcttc tcggcagagg agcgcaagaa tatcggcgtc
     2941 cacgtctgtc ctggcggtga cgtcgattct gtccacagcg ccgacgtgcc ctatgaggaa
     3001 ctcctgaagc acctcttcaa gctcaacgcc gggttcttcc agatggaaat ggccggcgag
     3061 cgcgatcgcg aatctgcgta tcagctggtg ggcaaatact tgcgggatga cgccaacggg
     3121 atcccgcaaa tggcctatat cggctgcatc gtgacccaaa gcccgcgcgc cgagtcagcg
     3181 caagaagttt gcgaccagtt agtcgctgcc gccaaatata tcgacaagca gcggcttggc
     3241 tcgaccgacg attgtggttt ctcgccgttc agtatcgaca cgaagcccaa tcacggctca
     3301 ctcgactacg ctcgtgacat cgcgtttcag aagatagcca atcgcgttgc cggaaccaaa
     3361 atggcagccg agaagctcgg ggttcactag gcatcttctg cgcgcttcgg tgcagtcgga
     3421 aacgccgatt agcgggaccg gctcctccgc ttgccgcttt ggcccattcc tccccggcca
     3481 ttggcggcac tgagccggcg gcggttttga aaaaatcgcc gtcggcgcga tttgcgtgtc
     3541 tgacttagga gggtggttac gccttcgcga gttgagcgaa gacatcatta gccttgagtc
     3601 gaaagccgtt ttgccgcctc ggaaatcctg tcgacgcagc aaaccatgcc atgtgcttag
     3661 acgctcgccg cttttttcaa ccctccactg gtccgggctc agcgttcgat cggcctccta
     3721 gcgtgctggg tttttgacgt cgcgagaaag cctacttgat caagcgccgg ttcggtggcg
     3781 gctttcggct ttgagatcac actccgacgg aatgtgcttc catcccctgt gcatttcaaa
     3841 atcctgaaac gcgtcttgta tgagtttgct gctctctgcg acgatccgaa gatcaccttg
     3901 accttgcggc acagggtagc ctgtatgatc cgtccatccc tcccgtaagc cggaacagat
     3961 aggctacatc atgcgcttcg tcatgaaccc cgatatggtt taccagtacc ctatctgggc
     4021 cgtcggtctg ctgcttgtcg gcgccgctgt gctcggcgct gtgctgcttg aactgtgcgc
     4081 gcgccgactg ttgccgatcg accttcgaca gcggcacaac gacgtggccg ccgcgatctt
     4141 ctccatcatc ggcgtgacgt acgccgttct gttggcgttt gtggccatgc tcgcatggga
     4201 aggcttcaat ggggcgaagg cggcagttat gccgaggctg cacggatcgg ggacgtctac
     4261 aatttgtcag ccggctttgc ggatctcgaa aagtcgtcga tccggaacga gatcttcggg
     4321 tatgcgcgac gtgttgtgac tgtcgaatgg c
//
LOCUS       DATMPU010000382         2515 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_1850947,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000382 DATMPU010000000
VERSION     DATMPU010000382.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 2515)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2515)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2515
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1850947"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..352)
                     /gene="modA"
                     /locus_tag="VNY06_08730"
     CDS             complement(<1..352)
                     /gene="modA"
                     /locus_tag="VNY06_08730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011090881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0030973 - molybdate ion binding [Evidence
                     IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HWX14908.1"
                     /translation="MAITALVVQAHGAEEKSITVFAAASMKNALDEVDTLFTNHSGIK
                     VVASYAASSALMMRIEQGASADVFLSANTEWMDYGAKRNLIKNDTRENLLRNRLVLTA
                     PKDSKIDEVSIAPGF"
     gene            577..921
                     /locus_tag="VNY06_08735"
     CDS             577..921
                     /locus_tag="VNY06_08735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011511791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HWX14909.1"
                     /translation="MAETQVSIRIDLETGGRIGPGKIALLEAIRKTGSITAAAQSMQM
                     SYRRAWLLVDELNKLMSEPVVTTTAGGAMHGGTTLTPVGEKTIALYHSIEAQIRAAAR
                     GEFQAFRKLMRG"
     gene            951..1100
                     /locus_tag="VNY06_08740"
     CDS             951..1100
                     /locus_tag="VNY06_08740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX14910.1"
                     /translation="MVAAVIGSAVVPTLIANAFYLPRHLLPDATNGLDTKGTTPSGVV
                     TARKV"
     gene            1110..1469
                     /locus_tag="VNY06_08745"
     CDS             1110..1469
                     /locus_tag="VNY06_08745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011441833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF190 domain-containing protein"
                     /protein_id="HWX14911.1"
                     /translation="MDVPKDAVLLRIFFGEEDRFKGKPLYEAIVQKAREMHLAGATVL
                     RGPMGYGHSSRLHTAKVLRLSFDLPIVVEIVDTEAKINEYLPILDGMMASGLVTLEKV
                     QVLQYGPEGLKERAGLA"
     gene            complement(1694..2074)
                     /locus_tag="VNY06_08750"
     CDS             complement(1694..2074)
                     /locus_tag="VNY06_08750"
                     /inference="COORDINATES: protein motif:HMM:NF025742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnA antitoxin family protein"
                     /protein_id="HWX14912.1"
                     /translation="MHEAGKPVRRGRPPSPSRKRAVKLRLDPEVLTSFRATGRGWQTL
                     MNAVLVAAAFRLQKSGARFAFGGLDPSDPDWRMQLHALLRAMMKAPRAKKKAAPRRGA
                     RHRPVRSPTQRSANRLRSENNASE"
BASE COUNT          545 a          736 c          708 g          526 t
ORIGIN      
        1 cgaacccagg cgcgatactc acctcatcga tcttcgagtc ttttggcgcg gtcaaaacca
       61 gtcgattgcg gagaagattc tcccgcgtgt cgtttttgat gagatttcgc ttcgcgccgt
      121 aatccatcca ttcggtgttg gccgaaagaa acacgtcggc tgaggcacct tgctcgatac
      181 gcatcatcaa tgccgagcta gcagcatagc tggcgacgac cttaatcccg ctgtggttgg
      241 taaacagagt atccacttca tcaagtgcat ttttcatgga cgccgcagcg aaaacggtga
      301 tcgacttctc ctctgcccca tgcgcctgca ccaccagcgc cgtaatcgcc accgtaaaag
      361 cagccagaac actccgtctc gcaagcacga ctctcctcca acaaacgcct gtttcgagtc
      421 ctgcgtggct tagttgtcca cgtaatcaac cgatgtccag agtgacgaga atgggcaaag
      481 gccggttata tgcgaacata gcacaagtca gagagagctg gcaccgtcgc tacagatgta
      541 cctcggaaca tcgattccac aggaagggtg tttcctatgg cagaaaccca agtttcaatc
      601 cgcatcgatc tagaaaccgg agggcgcatt ggcccaggaa agatcgctct gttagaggcg
      661 atccgcaaaa ctggatcaat cacagcagcc gctcagtcca tgcaaatgtc ttatcgtaga
      721 gcgtggctac tagtggatga gctaaataaa ctcatgagtg aaccagtggt caccactacc
      781 gcaggaggcg cgatgcatgg aggcacaaca cttacgccgg tcggagaaaa gaccatcgcg
      841 ctttaccact caattgaagc gcaaatccga gctgccgcgc gcggcgaatt tcaggctttt
      901 cggaaattga tgcgcggctg agtgccggcc gcggagcacg ggattccggg ttggttgcag
      961 ccgtgatcgg cagtgcagtg gtgccgacct taatcgccaa tgcgttctac cttccgcgcc
     1021 accttctgcc ggacgccacg aatggacttg acaccaaagg caccaccccg tctggcgtcg
     1081 tcacggcgcg caaagtctag gaggacaacg tggacgtacc caaggacgca gttctgctgc
     1141 gcattttctt tggagaggag gatcgtttta agggcaagcc gctctacgaa gcaatcgtgc
     1201 aaaaggcccg tgaaatgcac ctagcgggag caaccgttct tcgaggccca atgggctacg
     1261 gtcactccag ccggttgcac actgcgaagg tgctgcgatt gtccttcgat cttcctatcg
     1321 tggtcgaaat cgtagacacc gaggccaaga tcaacgagta cctgccgata ctcgacggga
     1381 tgatggcaag tggtcttgta acgctggaga aggtgcaggt gcttcaatac ggccctgaag
     1441 gtctgaaaga gcgagccggg ttggcctgac atcacctcaa gccagcgcga agcattttca
     1501 gcaatgcgct gaatttctgc cgctccgcaa tcgcggtctt ggcacgctcg ccgtgacgat
     1561 attgcccgtt ccgttcgcca gggggggccg ccgctaccgc gtgcgccggc aaactagaaa
     1621 gcagaacagc ccgcgctgac tcagccgtgg ccgctggtgc gtttttcaag acggccgcta
     1681 ggggtatagc caactactca gatgcgttgt tctcagaccg aagtcgattt gctgaccgct
     1741 gcgtcggcga tcgcacgggt cgatggcggg ctcctcgtcg gggcgctgct ttctttttcg
     1801 ctctcggtgc tttcatcatg gctctcagca gagcatgaag ctgcattcgc caatccgggt
     1861 cgctgggatc gaggccgcca aaagcgaacc gcgcgccgga tttttgaagg cggaaagctg
     1921 cggcgaccag cacggcgttc atcagcgttt gccagcccct tccagtcgcc cggaaggacg
     1981 tgagcacctc gggatcgagg cgaagcttca ccgcccgctt tcgcgacgga gatggtggtc
     2041 gcccacggcg aacaggcttg ccggcttcgt gcacgctggc ctcggcgagt ttctaatgct
     2101 tccgccgcta gcaagactaa ccgaatgggg gtttcggacc tcgccaagtc aatgctttgt
     2161 ctaatcagtc gcgcttggca acttgccatg aggtacttgc cagacacgtg acggccgctc
     2221 aatcagtagg ggaaaagcag ttcacgcagc ctcgcgacaa cgcagctggc ctcaatttat
     2281 tttgactgcg ccgtttcttg gccggtgata atgtcgccgc cggcggagcc gccctttttt
     2341 catttacaca aaagttccgg cagctttggc cgtcatccaa aaatcctgaa ggaggcgaac
     2401 atgtccaagc tgaaactcac gtccatgaat cgacgcgctt tcgtgatgag ctcggcggtt
     2461 ggagccctct cctcggcggc tttgctggct ttcaacaccg gcgccgccga cgcca
//
LOCUS       DATMPU010000383         4340 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_2786053,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000383 DATMPU010000000
VERSION     DATMPU010000383.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 4340)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4340)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4340
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2786053"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..796)
                     /locus_tag="VNY06_08755"
     CDS             complement(<1..796)
                     /locus_tag="VNY06_08755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012385038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HWX14913.1"
                     /translation="MREFRLRTGNPGQARSASPPGRQRRLRPLTLGIERIGLISLRYP
                     VTVALIAILLVTAAGLGIFGLKVDDSLSQLFRSQNSEFHQYEEVTRRFPSSEFDVLVV
                     IEGKKLLERASVEKLRDLATDLQLVDGTRGIISIFSARQPPRGQEIPAPLFPDPLPQG
                     SAYEATISQVMNNEIIRGKLLSEDGKLTLMVVALDPSAVENKRLSDVVGEIRKTIAED
                     FAGLRLTAELSGVPAMQLEIRNAVEHDRLVYNVVGFAAGCLIAIAFF"
     gene            complement(1084..1803)
                     /locus_tag="VNY06_08760"
     CDS             complement(1084..1803)
                     /locus_tag="VNY06_08760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020174407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HWX14914.1"
                     /translation="MRGLSEIKAKVADLQTHLGEAPGPLDDHSSLENRRSGQDVRTDK
                     EVKEKNTHRVIVDVAERLFRQLGFQKTTVADIARELNMSPANVYRFFAAKSEINAAVC
                     RDILGKIEAEAEKIAMTRGSAAQRLQSLLASVERSHHKRFMSDRKLHELVEVAISENW
                     SIMNEHNQRMAAILEQIIASGMAAGEFRPEDAAVAARLVNTACIRFCHPRLMVEYDQK
                     PEPTLDQMIGFCLAALTQPAN"
     gene            2278..3930
                     /gene="ettA"
                     /locus_tag="VNY06_08765"
     CDS             2278..3930
                     /gene="ettA"
                     /locus_tag="VNY06_08765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003611381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HWX14915.1"
                     /translation="MARQFIYNMRGLTKTYPGGKKVLENINLSFYPDAKIGVLGVNGA
                     GKSTLMRIMAGLDTEFTGEGFVAEGARVGYLPQEPALDDSLDVRGNVTQGVAEKKAIL
                     DRYNDLAVNYSDETADEMTRLQDEIEAKGLWDLDAQVDQAMDALGCPPDEADVAKLSG
                     GERRRVALCKLLLEQPELLLLDEPTNHLDAETVAWLEGHLRAYPGAILIVTHDRYFLD
                     NVTGWILELDRGRGIPYEGNYSAWLTQKQKRLTQEGREEEARKRQLEAESEWIASSPK
                     ARQAKSKARIQRYEELVQKQNDKAPTASQIVIPVAERLGNKVVAFSHLSKGFGDNLLI
                     DDLSFKLPPGGIVGVIGPNGAGKTTLFRMIVGQEKPDSGTIEIGESVHLGYVDQSRDS
                     LDGKKTVWEEISGGNEVLYLGKREINSRAYSSAFNFKGTDQRKKVSQLSGGERNRVHL
                     AKMLKSGANVLLLDEPTNDLDVDTLRALEEALTDFAGCAVIISHDRFFLDRIATHIMA
                     FEGDSHIEWFEGNFADYEEDKKRRLGIDSIIPHRLKYKKFSR"
     gene            complement(3945..>4340)
                     /locus_tag="VNY06_08770"
     CDS             complement(3945..>4340)
                     /locus_tag="VNY06_08770"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator
                     substrate-binding protein"
                     /protein_id="HWX14916.1"
                     /translation="QSFAGFVMMGASMLAVRDEPFILPTKGVNLQDLMNALISRGIRV
                     NVVAQTDNVYGALALVAAGIGVAVVPGHFEVASVKTVPVKDLNIERTIGLLWPRERKN
                     GPLKEFYSICRRPLRDGLRHFGRAKVLSL"
BASE COUNT          984 a         1248 c         1170 g          938 t
ORIGIN      
        1 ggaagaaggc gatcgcgatc aaacaacccg cagcaaatcc cacgacattg taaaccagcc
       61 tgtcgtgttc gaccgcattg cgaatttcga gctgcatggc cggtacgccg gaaagctcag
      121 ccgtcaaacg cagtccggca aaatcctcgg cgattgtctt gcggatctcg cccacaacgt
      181 cactaagtct cttattttca accgccgacg ggtcgagtgc gacaaccatc aaagtcagct
      241 tgccgtcttc cgataagagc ttgccacgaa tgatctcatt attcatcacc tggctgattg
      301 tcgcttcata ggcggatccc tggggaagtg gatcaggaaa caaaggcgcc ggaatttcct
      361 ggccgcgagg cggctggcga gccgagaaaa tcgaaatgat tccgcgcgtg ccgtcgacca
      421 gctgcaggtc ggtggcgaga tcgcgcaact tctcgacgga tgcgcgctcg agcaattttt
      481 ttccttcgat aacgacgaga acgtcaaatt cgctcgacgg gaatcgtcgc gttacttcct
      541 catattgatg gaattcagaa ttttgcgagc ggaaaagctg gctcagcgaa tcatcgacct
      601 tgagcccgaa aatgcccagt cccgcggctg taacgagcag aattgcgata agcgccacag
      661 tgacaggata gcgcaaagaa atcagtccta ttcgctctat tccaagggta agcggacgca
      721 gccttcgttg tcttccgggt ggagacgctg acctggcctg acctggattc ccggttcgca
      781 aacgaaattc cctcatcttg gccgtccctt agtccggtcc attcgggaag aacgagtatc
      841 gatagcgaac gcgcgccatg cgcttgacca ttagaacagc aagggagcct ggctacaggc
      901 tgccaaataa agcttccgtg ctgtgcctgc gtatcatcaa caaccccggg cagggccgga
      961 ttttatcccg tgatcacaac aatatgttgg gtgctggcag attcccctgc cgatcgggga
     1021 ccacgcttgg caggcggcgc tcatggcgaa accaagcgtg aagacgtttc tttagctacc
     1081 gactcagtta gctggctggg taagtgcagc gaggcagaaa ccaatcattt ggtcgagcgt
     1141 cggttcgggt ttttggtcat attcgaccat cagccgtgga tgacagaacc ggatgcaggc
     1201 ggtgttgacc aaacgtgccg caaccgctgc gtcctccgga cgaaactcgc ccgccgccat
     1261 gccgctggcg atgatttgct caagtatcgc ggccatgcgc tgattatgtt cgttcatgat
     1321 cgaccagttt tcgctgatcg caacttcaac cagctcgtgt agcttgcgat cgctcatgaa
     1381 ccgcttatgg tgggaccgtt ccaccgatgc gagcaggctt tggagccgct gagcagcgct
     1441 gccgcgagtc atggcgatct tttccgcttc ggcctcgatt ttgccgagaa tgtccctgca
     1501 gacagcggca ttgatttcag attttgcagc gaagaagcgg tacacattcg ccggcgacat
     1561 gttcagttcg cgtgcaatgt cggcgacggt tgtcttttga aagccaagct gccggaaaag
     1621 tctctccgca acatcgacaa taacccgatg cgtgttcttt tccttgactt ccttatcggt
     1681 acgcacgtcc tgaccactcc gccggttctc cagggaacta tggtcatcaa gcggaccggg
     1741 cgcttccccc aaatgggttt ggaggtcagc aacttttgcc ttgatttcac tcagaccgcg
     1801 catttgactc ttgtccatat tcaaaagggt aggcgactat gacctgattc tctttcccgc
     1861 gcttcaccca aatgggtttg gcgattagtt gctccataaa tatggttggt tgccgtacaa
     1921 aaacgctgta atatctgtca tcaattaata gtgagtgccg cctagaccgc ttcgttcggc
     1981 cggttcccac ttcacctccg tcctagctcg cgaaatccca attctgcggc ccgggaccgc
     2041 atagtgcgct gcagcattcg gaagacttcc tggaaacgac agcgcggccg ttagcattac
     2101 ggcgcgccac caaggtaatt ttggtcgaaa tgacagcagc gatatttttt cgcgccaggg
     2161 tcgggacgag ggctgctacc tctttggctg gcttgaaatc gcgccgcgac ctggaccggc
     2221 gcagcccatg cggctaaacc cctcgcatcg aaggctcttg cggaaagacc aaattccatg
     2281 gcgcggcaat ttatttacaa tatgcgcggc ctgaccaaga cctatccagg cggcaaaaaa
     2341 gtcctcgaaa atatcaatct ctctttttat cccgatgcca aaatcggcgt tctcggcgtc
     2401 aacggcgcag gcaaatcgac gcttatgcgg atcatggcgg gccttgatac ggaatttacg
     2461 ggggagggtt ttgtcgctga aggagcccgc gtcggctatc tgccgcaaga gcccgcgctc
     2521 gatgacagcc tcgatgtccg cggcaatgtc acgcagggcg tggcggaaaa aaaggcgatt
     2581 ctcgaccgct acaatgatct tgcggtcaat tattccgacg aaaccgccga tgaaatgacg
     2641 aggctgcaag acgagatcga ggccaaaggt ctttgggacc tcgatgccca agtcgaccag
     2701 gcaatggacg cgctcggctg ccctccggac gaagcagatg tcgcaaaact ctccggtggc
     2761 gaacgccgcc gcgtcgcgct ttgcaaattg cttctcgagc agccggagct tctgcttctc
     2821 gacgagccaa ccaaccatct cgatgcggaa acggtggcct ggctcgaggg ccatctgcgc
     2881 gcttatcccg gcgcaattct gatcgtcacc cacgaccgct attttctcga caatgtcacg
     2941 ggctggattc tcgagctcga tcgcgggcgc ggcattccct atgagggcaa ttattccgcc
     3001 tggctcacgc aaaagcagaa gcgcctcacc caagaaggcc gcgaggagga agcgcgcaaa
     3061 cgccagctcg aagccgagtc cgaatggatc gcgtcgagcc ccaaggcgcg gcaagccaaa
     3121 tccaaggccc gcatccagcg ctatgaggag ctcgtccaaa agcagaatga caaggcgcca
     3181 accgcctccc aaatcgtcat tccggtcgcc gagcggcttg gcaacaaggt cgtcgcgttt
     3241 tcgcatcttt ccaagggttt tggcgacaac ctgctcatcg acgatctgtc gtttaaactg
     3301 ccgcccggcg gcatcgtcgg cgtgattggc cccaatggcg caggcaagac cacgcttttt
     3361 cgcatgatcg tcggccagga aaagccggat tccggcacga tcgaaatcgg cgaatcggtg
     3421 cacctcggct atgtcgatca atcgcgcgat tcactcgatg gcaaaaaaac cgtttgggaa
     3481 gaaatttcgg gcggcaatga agtcctctat ctcggcaaac gcgagatcaa ttcgcgcgcc
     3541 tattcgtcgg cctttaattt taaaggcacg gatcagcgaa aaaaggtttc tcagctttct
     3601 ggtggcgagc gcaaccgcgt tcatcttgcc aaaatgctga aatccggcgc gaacgtcctt
     3661 ttgctcgacg agccgaccaa tgatctcgat gtcgatacgc tccgcgcgtt ggaagaagcg
     3721 ctcacggatt ttgccggctg cgcggtcatc atctcgcatg accgcttttt tttggaccgc
     3781 atcgcgaccc atattatggc cttcgagggc gacagccaca tcgaatggtt tgaaggcaat
     3841 tttgccgatt acgaagaaga caaaaagcgc cgcctcggga tcgacagcat catcccgcac
     3901 cgcctaaaat ataagaaatt ttcgcggtaa aagtgggggc ggggtcaaag tgaaaggact
     3961 ttggcgcggc cgaaatgcct taagccgtca cgcaatggcc ttcggcaaat cgaataaaac
     4021 tctttgagcg gaccgttttt ccgttcgcgc ggccatagga gaccgatcgt cctctcgata
     4081 ttcagatcct tgaccggtac cgtctttaca gacgcgacct cgaaatggcc gggaacaacc
     4141 gcgaccccga ttccggccgc gacaagtgcg agcgccccgt acacgttgtc cgtctgagcg
     4201 accacattta cccttatgcc gcgggaaata agtgcgttca tcagatcctg taagttaacg
     4261 ccttttgtgg gcaaaataaa cggctcatct cgaaccgcta acattgaggc tcccatcatg
     4321 acgaacccgg caaacgattg
//
LOCUS       DATMPU010000384         3057 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_1851275,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000384 DATMPU010000000
VERSION     DATMPU010000384.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 3057)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3057)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3057
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1851275"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..353)
                     /locus_tag="VNY06_08775"
     CDS             complement(<1..353)
                     /locus_tag="VNY06_08775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010684187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HWX14917.1"
                     /translation="MYMPHEVQTTEARYRRADLSRRRILKQAGWAMIAAACRPWRALA
                     ADQVSPAMSKLSAYMAQARTRALPEEAIEKTKHHLLDTIASMVSGSELPPGKAAIQFA
                     RAYGGEKVATVVASNV"
     gene            566..1210
                     /locus_tag="VNY06_08780"
     CDS             566..1210
                     /locus_tag="VNY06_08780"
                     /inference="COORDINATES: protein motif:HMM:NF025033.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HWX14918.1"
                     /translation="MNPAAFVAEAIADFGTVGAIAPSSRYLTQAMLRPLPLGNAQVVV
                     ELGPGTGVMTRAMLNVMPSNATLLTFEINPRFARHLRSTIKDPRLVVIHARAEELLEE
                     LSRRGYRHVDAMLSSLAMGLMSDRERHALLSEIGLSLRKKGTFTQYQYLHGLQFRDRQ
                     LSKFDMFGLLVRYFRSVQRTITWRNLPPAFVFTCRGARLSAPQDSIEASAFMKK"
     gene            complement(1256..2011)
                     /locus_tag="VNY06_08785"
     CDS             complement(1256..2011)
                     /locus_tag="VNY06_08785"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HWX14919.1"
                     /translation="MRAMVLAAGLGTRLRPLTDHRPKALVEVGGRTLLEIALARLASF
                     GIGEVIVNVHHFADMIVDYLKANKNFGMRVEISREDDVLLDTGGGLKKAAYFFLQNSQ
                     SPEEPFILHNVDVISTIDLRRMAQFHSANHALATLAVQDRPTSRYLLFDEQFQLCGRK
                     SGKEGQAELVRSSSHPKALAFSGIHVISPRLFSQMTEEGVFSIITPYLRLAAQGEKII
                     AFRSDEYQWRDLGRIDDVTQADQEARQLSGRPK"
     gene            complement(2008..>3057)
                     /locus_tag="VNY06_08790"
     CDS             complement(2008..>3057)
                     /locus_tag="VNY06_08790"
                     /inference="COORDINATES: protein motif:HMM:NF015620.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HWX14920.1"
                     /translation="LNYNVCYPRSSFDRQSIAWDLNYFKYYFLRLAGVSFNEQALEDD
                     FVRLADFLLQADGDYFLYRDFQSRNVMLRDACPWFIDYQGGRKGALQYDIASLLFDSK
                     ADLPPELRQQLLDNYLDRLSAFIHLDRSVFMRHYYAYVYIRLMQALGAYGFRGFYEQR
                     THFLQSVPYALKSLRWLLEHVELPIVLPTLMAAFRGMLNSEKLLTLSAESESTAETKN
                     LTVRVFSFSFHRGSPKDETGNGGGFVFDARSLPNPGREERFKPLTGKDAPVIDYLNGQ
                     ESVHRFLAGATSLVDASLSNYQSRGFKNLMVSFGCTGGQHRSVYLAEALAKHLRAHNG
                     INVVVRHIELEKQGK"
BASE COUNT          674 a          831 c          928 g          624 t
ORIGIN      
        1 acattggaag caactaccgt ggcaactttc tcgccgccat atgcgcgggc gaattgaatc
       61 gcggccttgc cgggaggaag ttcggagccc gaaaccatgg aagcgatggt gtccagcagg
      121 tgatgcttcg ttttctcgat ggcttcctcg ggcaacgcac gagtgcgcgc ctgggccatg
      181 taagcgctca gcttggacat cgcgggactg acctggtccg ccgccagcgc ccgccagggc
      241 ctgcatgccg cggcgatcat cgcccagccc gcctgcttca aaatgcgacg gcggcttaga
      301 tccgcgcggc gatagcgcgc ctcggtcgtt tgcacttcat gtggcatata catccgcctt
      361 tccggtatct actaatcagg cttattaata ccacgaaaaa tcggtgcgaa tgcgatccaa
      421 ggaacccgga ttaaggctgg cttcagcttc acatgattat gtaaggccag gattgtagat
      481 acgcgtcgga gcaatcaaga aagtttaaag cagtgacggc gccagcatcg tggggtaagc
      541 tggtctgtat ggcggggatg acaaaatgaa tccggcagca tttgtcgcgg aagcgatagc
      601 cgacttcggc acagttggcg cgatcgcgcc cagttcgcgg tatctgacgc aggccatgct
      661 gcggccgctg ccgctgggaa atgcgcaggt ggtggttgag ttggggccgg gcaccggcgt
      721 catgacgcgg gccatgctga acgtcatgcc ttctaacgcg actttgctga cgtttgagat
      781 caatccacgg tttgctcgac acctgagatc gacgatcaag gaccctcgcc tcgtggtcat
      841 ccacgcgcgg gcggaagagc tgctcgaaga gctgagccgg cgcgggtacc ggcacgtcga
      901 tgcgatgctt tcgtcactgg cgatgggctt gatgtcagat cgcgaaaggc acgcattact
      961 gagcgaaatc ggtctcagcc tcaggaagaa aggcacgttc acgcagtatc aatacctgca
     1021 tggtttgcaa ttcagggacc ggcaactgag caagttcgat atgttcgggc ttctggtgcg
     1081 ctattttcgc tctgtgcaac ggacgattac gtggcgcaac ctgccgcccg ccttcgtctt
     1141 cacctgcagg ggagcccggc tcagcgcccc gcaagactca atcgaagcgt ccgcattcat
     1201 gaaaaagtaa gcgggctctc gccgtcgcga cttgataaat gggcgccggt tgaatctact
     1261 ttggccggcc cgaaagctgc ctcgcctcct gatcggcctg cgtcacgtca tcaatcctgc
     1321 ccagatcacg ccactgatac tcatcggacc gaaacgcgat gattttttcg ccctgtgcgg
     1381 caaggcgcag gtaaggcgtg atgatggaaa agacgccttc ttccgtcatc tgcgagaaaa
     1441 ggcgcggaga aatgacgtga atgccggaga aagccagcgc cttggggtgc gatgagctgc
     1501 gaaccagttc ggcctggcct tcctttcccg atttccggcc gcaaagctgg aactgctcgt
     1561 caaagagcag atagcggctg gtggggcgat cctgcactgc aagcgtcgcg agagcgtgat
     1621 ttgcggaatg aaattgcgcc atgcggcgaa gatcgatcgt gctgatcaca tcgacattgt
     1681 ggaggatgaa gggctcttcg ggagactgcg aattctgcaa gaagaagtaa gcggcttttt
     1741 tcaagccgcc gccggtgtcg agcaaaacgt cgtcctcgcg ggaaatttcg acgcgcatgc
     1801 cgaaattttt gttcgccttc aggtaatcga cgatcatatc cgcgaagtga tgcacgttga
     1861 cgatcacttc gccaatgccg aaggaggcga ggcgcgcgag cgcgatttcc agcagcgtgc
     1921 ggcccccgac ttccaccaga gccttgggac gatgatccgt gagcggccgc aaacgcgtac
     1981 ccaggccggc ggccaaaacc atggccctca tttgccctgc ttctcgagtt cgatgtgacg
     2041 gacgactacg ttgatgccgt tgtgggcgcg caggtgtttt gccaatgctt cagctaaata
     2101 gaccgagcgg tgctggcccc cggtgcagcc aaacgagacc atcagattct tgaagccgcg
     2161 gctctgatag ttgctcaagc tggcgtccac aagcgaagtg gcgccggcta aaaaccgatg
     2221 cacgctttct tgcccgttga gatagtcgat gaccggcgcg tctttgccgg tgagaggctt
     2281 gaagcgctct tcgcgtccgg gattcggaag actgcgggcg tcaaaaacga atccgccgcc
     2341 gtttccggtc tcgtcttttg gcgagcctcg gtgaaaagaa aagctgaaaa ctcggacggt
     2401 caggttcttt gtctcggctg tcgattccga ctcagcagac agggtcagca atttctcgga
     2461 attcagcatg cctctgaacg cagccatcaa ggtgggcagc acgattggca actcaacgtg
     2521 ttccagcagc cagcgcaagc ttttcagcgc gtaagggacg ctttggagaa agtgggtcct
     2581 ctgctcgtaa aaaccgcgaa agccgtacgc gcctaacgcc tgcatgaggc ggatgtaaac
     2641 gtaggcgtag tagtggcgca tgaagacgct gcggtctagg tggataaaag ccgagagccg
     2701 gtcaagataa ttgtcgagca actgctggcg gagttcgggc ggcaggtcgg ccttagaatc
     2761 aaacaaaagc gaagcgatgt cgtattgcaa tgcgccttta cggccgcctt gataatcgat
     2821 gaaccaagga caggcgtcgc gcaacatgac gttgcgtgac tggaaatcgc ggtagaggaa
     2881 gtaatcgccg tcggcttgca gcaggaagtc cgcgagacgg acgaaatcgt cttcgagcgc
     2941 ctgctcgttg aaggaaacgc ccgccaacct tagaaaatag tatttgaaat aattcaaatc
     3001 ccaggcgatg gactggcgat cgaagctgga acgcgggtag cagacgttgt agttcaa
//
LOCUS       DATMPU010000385         2577 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_4316781,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000385 DATMPU010000000
VERSION     DATMPU010000385.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 2577)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2577)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2577
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4316781"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..179)
                     /locus_tag="VNY06_08795"
     CDS             complement(<1..179)
                     /locus_tag="VNY06_08795"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: protein motif:HMM:NF015647.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HWX14921.1"
                     /translation="MARAFEHLPASPIRAIVCGGGTRNKTLMRELAQRLPCAVAPAES
                     FGWPSDAIEAKAFAYL"
     gene            complement(106..957)
                     /locus_tag="VNY06_08800"
     CDS             complement(106..957)
                     /locus_tag="VNY06_08800"
                     /inference="COORDINATES: protein motif:HMM:NF015647.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HWX14922.1"
                     /translation="MSGTSMDGVDVAIIETDGQESVTLGRTGFFPYSETDRALLRRAV
                     TEAVSLDDRSARPGSLARADAVIAVRHAEAVEAFLSAESMSPEEVDLVGFHGQTVLHR
                     RDRRLTVQIGDGPALAERLGIKVVFDFRAADVAEGGQGAPLVPIFHRALAAAAGFAEP
                     VAFINIGGVANLSFIAPGEDPIACDTGPGNALLDDLMLKRRGLPFDRDGAIAASGQAD
                     AATLDKLLAHPFFLAPPLGRSCRRADDGGCRSDAYCLHSGKHSARVRAFAGKSHSRHR
                     VWRWHSK"
     gene            1240..>2577
                     /gene="tyrS"
                     /locus_tag="VNY06_08805"
     CDS             1240..>2577
                     /gene="tyrS"
                     /locus_tag="VNY06_08805"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012590634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HWX14923.1"
                     /translation="MTGDFRPQSGLLAVLIERGFLHQCSDLTSLDQKAKSGELVAYIG
                     FDCTAPSLHAGSLVQIMLLRWLQKTGGKPIALMGGGTTRVGDPSGKDASRQLLSLETI
                     ATNKVGIKRIFAKFLRFGEGKQDAIMPDNAEWLGGLNYIDFLRDAGRHFSVNRMLTMD
                     SVKLRLERDQELSFLEFNYMCLQAYDFVELHKRYGCVLQMGGSDQWGNIVIGIDLGRR
                     MGTGQLYGLTSPLLTTASGAKMGKTAQGAIWLDETMLPVFEYWQYWRNCEDRDTGRFL
                     KLFTELPLDEIGRLESLGGAEINEAKKILATEATSMVHGREAALRAAEDARQTFVYSE
                     ARAASIDQGIRLQGIPSAEAFGTPAIIAGLPSVAIPAAKIEAGLGVLTAFVKSGLVAS
                     TSEARRQIKAGGLKINDHAVTDERAVIGSGDFKNGVVKLSLGKKRHILLKVNGR"
BASE COUNT          550 a          777 c          724 g          526 t
ORIGIN      
        1 agataggcga atgccttagc ctcgatcgcg tcgctcggcc agccaaagga ctccgccgga
       61 gcgaccgcac atggcagccg ttgagccaat tctcgcatca gggtcttatt tcgagtgcca
      121 cctccacaca cgatggcgcg aatgggactt gccggcaaat gctcgaacgc gcgcgctatg
      181 ctttccgctg tgaaggcagt aagcgtcgct gcggcatcct ccgtcgtcag ctcttcgaca
      241 agagcgcccg agcgggggtg ccaagaaaaa aggatgtgcg agaagcttat ccagagtcgc
      301 cgcatcggcc tgaccgcttg cggcgatggc gccatcacga tcgaacggca ggcctcggcg
      361 ttttaacatg agatcatcga gcagcgcatt gccggggcca gtatcgcaag ctatcggatc
      421 ctcccctggc gcgataaaac tcaaattggc cacgccgcca atattaataa atgcgaccgg
      481 ctcggcgaaa cccgctgcgg ccgcaagcgc gcggtgaaat ataggcacga ggggcgcccc
      541 ctgtccgcct tctgccacgt cggccgcgcg aaaatcaaaa accactttga tccctaatct
      601 ttcagcaaga gctgggccgt cgccgatctg aacagtgaga cgccgatctc gccgatgcag
      661 aacggtctgc ccgtggaaac ccacaaggtc gacttcctcg gggctcatcg attccgcgga
      721 cagaaaagct tcaacagcct ccgcatggcg gacggcgatc acggcgtcgg cccgcgccaa
      781 agagcccgga cgtgcgctgc gatcgtctag agagaccgct tccgtgacgg cacgacgcag
      841 caaagcccgg tccgtttcgc tgtaaggaaa aaaaccggtg cggccaaggg tgacgctctc
      901 ttgaccatcg gtctcgatga tggcgacatc gacaccatcc atcgaggttc cgctcatcag
      961 gcctatcgcg cgaaccactt gaccaggctc tctgcccgcc tttgccggca aacaatgttg
     1021 ctgagcttgt cgcaactcgc tcccattccc cgcacctttc taaacggaga cgggacgcgc
     1081 ccttgaccac acaggagggc tttgcagtcc tgctccgcgg ctctctaact ccgttcttgc
     1141 aatgctccgc ccttccctct agatctcggc gcctcttgtt tttagtaaga tagcgcaact
     1201 ttgatgtgct gccctacttc ttttgcgtga aggcttcccg tgaccggcga ttttcgtccc
     1261 caatccggct tgctcgccgt actgatcgag cgtggctttc tgcatcaatg ctccgatctt
     1321 accagtctcg atcagaaagc caagagcggc gaacttgttg cctatatcgg ttttgattgc
     1381 accgcgcctt cgcttcatgc cggctcgctc gtccagatta tgctgctgcg ctggctgcaa
     1441 aaaaccggcg gcaaaccgat agcgctcatg ggcggtggga cgacgcgggt cggcgaccct
     1501 tctggtaagg atgcaagccg ccaattgctg tctctcgaaa cgatcgccac caataaagtc
     1561 ggcataaagc gcatctttgc caaattcttg cgctttggcg aaggaaagca ggacgcgatc
     1621 atgccagaca atgcggagtg gcttggcggc ctcaactaca tagatttctt gcgcgatgcg
     1681 gggcgccatt tctcggtcaa ccggatgctt acgatggatt ccgtaaaact ccggctcgag
     1741 cgggaccagg aactgtcttt tctcgaattc aactacatgt gcctgcaggc ttacgacttt
     1801 gtcgagcttc acaagcgtta tggctgcgtc ttgcagatgg gcggctcgga tcaatggggc
     1861 aatatcgtca ttggaatcga tctcggccgc cggatgggaa cgggccagct ctacggcttg
     1921 acgtctccgc tgttgacaac ggcgtctggc gccaaaatgg gcaagaccgc acaaggggca
     1981 atttggctcg acgaaaccat gttgccagtg ttcgaatatt ggcaatattg gcgcaattgc
     2041 gaggaccgcg ataccggacg gtttttgaag ctcttcaccg aactcccgct cgatgagatc
     2101 ggccggctcg aatcgctcgg cggtgctgag ataaacgagg cgaaaaaaat tctcgccacc
     2161 gaagcaacct ccatggtcca tggccgggag gcggcattgc gcgcagccga ggacgcacgc
     2221 caaacttttg tatactctga ggcacgagca gcatcgatag atcaaggcat ccggctacaa
     2281 ggcatccctt ccgccgaggc atttgggacg ccggcgatca tcgcgggact gccctcggtc
     2341 gcaatccccg ctgccaaaat cgaggctgga ctcggcgtgc ttactgcttt cgtcaaatcg
     2401 ggccttgtcg cctccacaag cgaggcgcgg cgtcagatca aagccggcgg cctcaagatc
     2461 aatgatcacg ctgtgacaga cgaacgtgct gtcatcggct cgggagattt caaaaacgga
     2521 gttgtaaagc tgtcacttgg gaaaaagcgg cacattctgt tgaaggttaa cggccgc
//
LOCUS       DATMPU010000386         4258 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_2474048,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000386 DATMPU010000000
VERSION     DATMPU010000386.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 4258)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4258)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4258
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2474048"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(312..1256)
                     /gene="murB"
                     /locus_tag="VNY06_08810"
     CDS             complement(312..1256)
                     /gene="murB"
                     /locus_tag="VNY06_08810"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019403404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HWX14924.1"
                     /translation="MLSQDRSLGLTFKDIVPDLRAQMPDLRGCLIGNAPMAPLSWFRT
                     GGQAQVLYEPADESDLAYFLGGLEQAIPVRVLGAGSNILIRDGGIEGAVIRLGKAFQR
                     LKIDGLTLRAGAGVPDVKLSSAAANAGISGLAFFRGIPGTIGGALRMNAGAYGAETAD
                     VLVSSRGINRKGAMVAFSGADMGFSYRHCGVAEDVVLTEAVFTGGSGDPEKILAEMVG
                     ITRVRSVTQPVNTRTGGSTFKNPPGQKAWELIDKAGCRGLAIGDAQVAELHCNFLINR
                     GNATAADLETLGETVRTRVHETSGITLEWEILRMGRAP"
     gene            complement(1240..2664)
                     /gene="murC"
                     /locus_tag="VNY06_08815"
     CDS             complement(1240..2664)
                     /gene="murC"
                     /locus_tag="VNY06_08815"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012592446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HWX14925.1"
                     /translation="MRLPDKLGPIHFVGIGGIGMSGIAEVLLNLGYKIQGSDAYENAN
                     VARLRAKGALIHIGHHAQNLDGAQVVVVSTAIKRDNPELIAARMNRLPVVRRAEMLAE
                     LMRLKHCVAIAGTHGKTTTTSLVATLLEAGKFDPTVINGGIINAYGTNARLGSGDWMV
                     VEADESDGTFLKLPADIAIITNIDPEHLDHFKTFDAIKAAFRAFVENLPFYGFAVMCA
                     DHPVVRELAARIEDRRVITYGENPDADVRLTNLDLKNGVSRFTVIIRDQENGAKTTLE
                     NIMMPMPGHHNALNATAAITVAHRLGISAETIRVALGEFGGVKRRFTRVGEWNGAVIF
                     DDYGHHPVEIVAVLRAARASTEGRVIAIVQPHRYTRLHSLFDGFICAFDDADVVIVAD
                     VYSAGEAPIEGSSRDALVIGLKAHGHRHALPLTRPEDLPALIRSLAKPGDYIVFLGAG
                     NITQWAHALPGQLAAEALCDALAG"
     gene            complement(2661..3773)
                     /gene="murG"
                     /locus_tag="VNY06_08820"
     CDS             complement(2661..3773)
                     /gene="murG"
                     /locus_tag="VNY06_08820"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019403406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HWX14926.1"
                     /translation="MSGRCRIVVAAGGTGGHLFPAAAVSHALIARGFEIHLVTDERAA
                     KYGGDFPAQDIHRVAAASPSGGSVFSRARAVLTLIQGTLAARKLFKKIEPGAVVGFGG
                     YPAVPPVLAAARLRVPTLLHEGNAVIGRANRFLAPRVDAIAKGFEVLGDLPESLAART
                     HLTGNPVRPMVLEAARMPFPDWSDGRLKILVTGGSQGARIFSDIVPAAIALLPEEARK
                     KLMLVQQARGEDEERVRAAYAGLGVAAEVRSFFPDLPARIAQAHLVIARSGASTISEL
                     AVIGRPAILVPLPHALDQDQAANAKQFAATGAATIVTQSVFSPQCLATSLIQAQGNMG
                     ALAARAAAAKRAGVPDAAERLANLVQGLLSNKDKST"
     gene            complement(3770..>4258)
                     /locus_tag="VNY06_08825"
     CDS             complement(3770..>4258)
                     /locus_tag="VNY06_08825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012383699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HWX14927.1"
                     /translation="PGAAGGGIADTFQVDTALDCFLSGGWFGKGPGEGTVKRILPDAH
                     TDFIFAITGEEFGVLACLLIVAIFAFIVLRGLLAARRNEDPFCRFAAAGLVLLFGIQS
                     AINMAVNVHLLPAKGMTLPFISYGGSSLISLALAIGFLIAVLRKRPGSAILAQAGAEV
                     LA"
BASE COUNT          917 a         1277 c         1253 g          811 t
ORIGIN      
        1 catccgaacg aaccgtccag ggcacctcga caccaaagac gaatgtggtc gttatttcgt
       61 taatgcagga tacgcttcaa tatcaaatca tcccgcccta gagagcccaa aagtggcggt
      121 tttgggttcc ggacacacgc cagaacaaag gattagagca agatgacttt ccttcttctt
      181 gctctaggct agtgtcttcc gccttgcaag gttttgggcc ttaattggac aaaaatgttt
      241 cacgtgaaac attttggtaa ggttcgctgc gctttaaatt ttgccgaggc atgtgggatt
      301 gctggcggcg ttcacggcgc gcgacccatc cgcaaaattt cccattcgag ggttatgcca
      361 cttgtctcgt gcacacgcgt gcggacggtt tcaccaagag tctcgagatc ggctgccgtg
      421 gcattcccgc gattgatcaa aaaattgcaa tggagttcag cgacttgcgc atcgcctatc
      481 gcaaggcccc ggcaacccgc tttgtcaatc aattcccagg ccttttggcc cggcggattc
      541 ttgaaggtcg agccgccggt gcgggtgtta accggctgcg tcaccgagcg gacgcgggtt
      601 atccccacca tctcggccag tattttttcg ggatcgccgg aaccgcccgt aaacacggcc
      661 tctgtaagga ccacatcctc tgcgacgccg caatggcggt aagaaaagcc catgtccgcg
      721 ccggagaacg ccaccatcgc acccttgcga ttgatccccc gggaggagac aagaacatct
      781 gccgtttccg cgccataggc gccggcattc atccggagcg ctccgccaat cgtgccgggt
      841 atgccgcgaa aaaaggccag gccagagatg cccgcattgg cggcagcgga gctcagtttc
      901 acatccggca cgccagcgcc cgcgcgcaag gtcaggccat cgatcttaag gcgctggaac
      961 gctttgccaa ggcggatcac cgcgccctca atgccgccat cgcggatgag aatgttcgag
     1021 cccgcgccga gcacgcgaac cgggatcgct tgttcgagcc cacctaaaaa ataagcaagg
     1081 tcgctttcgt ccgcgggctc ataaagcact tgcgcctggc ctccagtccg aaaccaggac
     1141 aaaggcgcca tcggcgcgtt gcctattaga cagcctcgta ggtcgggcat ttgcgcccgc
     1201 aggtccggca cgatgtcttt gaaagttagg ccgaggctcc tatcctgcga gagcatcgca
     1261 cagcgcttcc gccgcaagct ggccaggcag cgcatgcgcc cattgggtga tgttgccggc
     1321 accaagaaaa acaatgtaat cgccgggctt ggcgagggag cggatgagtg ctggcaaatc
     1381 ctccggccgc gtgaggggga gcgcatggcg gtgaccatgc gctttgaggc caatcacgag
     1441 agcgtcgcgg ctcgagcctt caattggtgc ttcgccggca gaatagacgt cggccacgat
     1501 cacgacgtcc gcatcatcga acgcgcagat aaaaccatca aagagcgaat ggagccgcgt
     1561 gtaccggtgc ggctgaacaa tggcaatgac ccggccctcc gtcgaggcac gggcggcgcg
     1621 caagacagca acgatttcga ccggatggtg gccataatca tcaaaaatga ctgccccatt
     1681 ccactcgccg acgcgggtaa agcggcgctt gacgccgcca aattcgccaa gcgctaccct
     1741 gatcgtctcg gcgctgatgc caagccggtg cgccacagtg atcgcggccg ttgcgttcag
     1801 cgcattgtga tgcccaggca tcggcatcat gatgttttca agcgtggttt tggcgccatt
     1861 ttcctggtcg cgaatgatga ccgtgaagcg cgaaacgccg ttcttgaggt caagatttgt
     1921 gagccggacg tcggcgtctg gattttcgcc ataagtgatg acgcgcctgt cttcgatcct
     1981 ggctgcgagc tcgcgcacga cgggatgatc tgcgcacatg acggcgaaac cgtagaaggg
     2041 gagattttcg acgaaagcgc gaaaggcggc tttgattgca tcgaaagttt taaaatggtc
     2101 gagatgctct gggtcgatat tggtgatgat cgcaatgtcg gcgggaagtt tcaggaaagt
     2161 gccatcgctt tcgtcggctt cgaccaccat ccaatcgccg ctgccgagcc gcgcattggt
     2221 tccgtaagcg ttgatgatcc cgccgttgat gaccgtcgga tcgaatttgc cggcttcgag
     2281 cagagttgcg acgagcgacg tcgtcgttgt cttgccatgc gtgccggcga tggcgacaca
     2341 atgttttagc cgcatgagtt cggcgagcat ttcggcgcgg cgcaccaccg gcaggcggtt
     2401 cattcgggcc gcaataagct cggggttgtc gcgcttgatc gcggtcgaga caacaacgac
     2461 ctgcgccccg tcaaggttct gcgcgtgatg accgatatgg atcagcgcgc ccttggcacg
     2521 cagccgcgca acattggcgt tttcgtaggc atccgaacct tggatcttat agccaaggtt
     2581 cagcaaaact tcggcaatgc ccgacatgcc tatgccgccg ataccgacaa aatggatcgg
     2641 gccgagttta tccggcaatc tcatgtactt ttgtccttgt tgctcaaaag accctgcaca
     2701 aggttggcga gcctctcggc tgcgtcggga acgcccgccc gctttgccgc cgcggcgcgt
     2761 gccgcaagcg cgcccatatt accttgtgct tgaataagag aagtcgccag acattgcggc
     2821 gaaaatacgc tctgggtgac aatagtggca gccccggtcg ccgcaaactg cttcgcattc
     2881 gctgcctggt cctggtcgag agcatgggga agcggcacta agattgctgg gcgcccgatc
     2941 acggcgagtt ccgatatcgt cgaggcgccc gagcgagcaa tgacgagatg agcttgcgcg
     3001 atccttgccg ggagatctgg gaaaaaagat ctgacctcgg cggcgactcc gagcccggcg
     3061 taagcagcgc gcactctttc ctcgtcttct ccgcgcgcct gctgaacaag catcagtttt
     3121 ttccgcgctt cctctgggag aagggcaatc gcggcgggca cgatgtcgga aaaaatccgc
     3181 gcgccctgcg agccccccgt cacaaggatt ttgagcctgc catcggacca atccggaaat
     3241 ggcatccgcg ccgcttcaag gaccattggg cgaaccggat ttccagtcag atgcgtgcgc
     3301 gccgccaagc tctcgggcag atccccaagg acctcaaatc ctttggcgat ggcatcgacc
     3361 cttggagcga gaaagcggtt ggctcggccg atgaccgcgt tgccctcatg caggagcgtc
     3421 ggcacccgaa gccgcgccgc cgcgagcacc ggaggaacgg ccggatagcc gccaaagccg
     3481 acgaccgcgc caggctcaat ctttttaaaa agttttcgcg ccgccaacgt tccttgaatg
     3541 agagtcaaca ccgcccgagc gcgcgaaaag accgatccgc cgctcggcga ggcagcggca
     3601 acgcggtgaa tgtcctgcgc gggaaaatcg ccgccatatt tcgccgcccg ttcgtcggtg
     3661 acgaggtgaa tctcaaaacc gcgggcgatg agcgcgtgac tgactgcagc tgccggaaac
     3721 aaatggccgc ccgtgccgcc cgcggcgacc acgatccgac agcggccgct catgccaata
     3781 cttctgcccc tgcttgcgca aggatcgcag aacctggccg cttgcgcagc accgcgatta
     3841 gaaagccgat tgcgagcgcg agggaaatga gcgatgagcc gccataagaa atgaagggca
     3901 gcgtcatgcc cttggccggt agaagatgaa cattgacggc catgttgatc gcgctttgaa
     3961 taccgaaaag cagcaccagg cctgctgcag caaagcggca gaaagggtcc tcattgcgcc
     4021 ttgccgctaa taatccgcgc aaaacgataa aggcgaaaat cgcaacgatc aggagacagg
     4081 cgagaacacc aaattcctcc cccgtgatgg cgaaaataaa atccgtatgg gcgtctggaa
     4141 gaatgcgctt gaccgttcct tcgcctggcc ctttgccaaa ccagccgccg gacagaaaac
     4201 aatcgagcgc cgtatcaacc tgaaacgtgt cggcgatccc gccgccagca gcaccggg
//
LOCUS       DATMPU010000387         2507 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_4324240,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000387 DATMPU010000000
VERSION     DATMPU010000387.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 2507)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2507)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2507
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4324240"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..299)
                     /locus_tag="VNY06_08830"
     CDS             complement(<1..299)
                     /locus_tag="VNY06_08830"
                     /inference="COORDINATES: protein motif:HMM:NF013842.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 transposase-related protein"
                     /protein_id="HWX14928.1"
                     /translation="MGKAYSQDLRERVMAAVDAGTGAYAAASFFRVSVSYIYKALGRR
                     QRTGETSARPWAGGPKPKLAAHDDALRAHVMSKPDATLAELQAWLIAEHDVKVSAG"
     gene            complement(569..1426)
                     /locus_tag="VNY06_08835"
     CDS             complement(569..1426)
                     /locus_tag="VNY06_08835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011509095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HWX14929.1"
                     /translation="MEESSRKTAFVFAGGGSLGAIQVGMLRVLLSAGVQPDFVIGTSV
                     GAINAAYFAGSPNEKGVENLARLWFSLRRSDIFPFTLARVFGLLRQPDSLVDSNGLRQ
                     LIEDNLPYARLEDAAIPVYVTATSLEGIGILLSKGPAVDSVLASTAIPGIFPPVHLDG
                     DVLMDGAIANNTPILAAARLGASHIVVLPTGIACSLQEPPKGAIARALHGVTLLIAWQ
                     VIRDLEQIGEGVHVCLVPALCPLDVSPYDFSASRYLIQRAAETARKWLDGGGLTRRFQ
                     PHEFAPHRH"
     gene            complement(1461..1982)
                     /locus_tag="VNY06_08840"
     CDS             complement(1461..1982)
                     /locus_tag="VNY06_08840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX14930.1"
                     /translation="MARSIKHEQRLLSTDELTLVEKSHHPALGLLPDHDLKELRKLVR
                     ERRDRAQDIAARQRRELRGKSAPEGVRPATGDSGTREKRDLLAAALQRLNRETTRREA
                     KAARQELIDNAKRALELRRDAEAKSIWPSPGRTANEGINPKNSPTYSLRNSAKLGTIS
                     QHNKNMQAKRDSK"
     gene            complement(2056..>2507)
                     /locus_tag="VNY06_08845"
     CDS             complement(2056..>2507)
                     /locus_tag="VNY06_08845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007010894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="host attachment family protein"
                     /protein_id="HWX14931.1"
                     /translation="RESLMHFKLSHGAWVFVGDGQKALFLINEGDEKFPNLRRLSVLE
                     NPDPPTREQGSDAPGRAYASVGQIRSSVEETDWHELQKERFAASIAEQINKAAMSTAF
                     SQIVIVAPAKILGDLRREFSKETESKIVAEKGKDLTNHTIAEIERLL"
BASE COUNT          515 a          766 c          685 g          541 t
ORIGIN      
        1 ccggcgctga ccttcacgtc atgctccgct ataagccagg cttgcagctc ggcaagcgtc
       61 gcatcgggtt tgctcatcac atgggcgcga agggcatcgt catgcgcggc gagcttcggt
      121 ttggggccac cggcccaagg acgcgcgctc gtctctcccg ttctctgccg ccgccccaaa
      181 gctttgtaga tataggagac gctgacccgg aagaacgacg ccgcggcata agcgcccgtt
      241 cccgcatcca ccgccgccat cacccgctcg cgcaggtcct gcgagtaagc cttgcccatc
      301 acgattcccc gtcgaatcaa cgacaaaagg gaatcacaaa ctcagcacga ctgaaatccc
      361 aggcgattcc accaatcatg aaagtgctct agagcatttt ccgatcacgt tgcatcgtat
      421 ccggcggccc acttttggct caacacgctc tagaccggct agcggtgaaa tcacgggccc
      481 tgctgcggcc tatttgggaa cgcgctgggc tcgcatgctt tcattgtgct tgctgagctt
      541 catggaaatc gaaagcggcg tgatcaggtc aatggcggtg cggtgcaaat tcatgcggct
      601 ggaagcgacg ggtaaggccg ccgccgtcaa gccattttcg tgccgtctcc gccgcgcgtt
      661 gaataagata acgcgacgcc gaaaaatcat atggcgacac atcgagcggg cagagcgccg
      721 gcacgaggca aacgtggact ccctccccga tttgctcgag gtcacggata acctgccacg
      781 cgatcaacag ggtcaccccg tgcaacgccc gtgcaatcgc ccctttcgga ggttcttgca
      841 gggagcaagc tatcccagtt ggaagcacca cgatatgaga cgctccgagc ctggccgccg
      901 ccaaaatagg ggtgttgttg gcaatcgcac cgtccatcag cacgtcgcca tcaaggtgaa
      961 caggcgggaa aatgcccggt atcgcggtgc ttgcgaggac ggagtcgacc gccggtcctt
     1021 tggagagaag aattccaatc ccctcgaggg aggtcgccgt aacgtaaaca ggaatggccg
     1081 cgtcttccag ccgggcgtat ggcaaattgt cctcaatcag ctgccgcaag ccgttggaat
     1141 cgaccagact gtctggctgc ctgagcagac cgaagacgcg ggccaaggta aacggaaaaa
     1201 tgtcgctgcg tcgcaggcta aaccaaagac gagcgagatt ctcgactcct ttctcattgg
     1261 gagatccggc aaaataagca gcatttatcg cgccgaccga tgtgccgatg acgaaatctg
     1321 gctgcactcc ggcggatagc agtacgcgaa gcatcccgac ctggatcgcg ccgaggctgc
     1381 ctccgcccgc aaaaacaaat gctgtcttac ggctagactc ttccatacgg gcctagttca
     1441 aattcccgtc gccggggcat ctatttcgaa tcacgcttgg cctgcatatt tttgttgtgt
     1501 tggctgatcg tgccgagctt ggccgaattg cgcaacgaat atgtcggcga gtttttgggg
     1561 ttaattccct cattggccgt gcgccccggc gaaggccaaa tggatttggc ttcagcgtct
     1621 ctgcgaagtt cgagcgcgcg ctttgcattg tcgatcaatt cttgacgggc ggctttggct
     1681 tcgcgccgtg tcgtctccct attcaaccgc tgaagggcgg ccgccaaaag atcgcgtttt
     1741 tcgcgcgtgc cgctgtctcc cgtagcaggg cgtacgcctt caggcgcgga ctttccgcgc
     1801 agctcccgcc gctgacgcgc ggcaatgtct tgcgcccggt cacggcgctc gcgcacgagc
     1861 ttacgcaatt ctttcaaatc atgatccggc aacaaaccca gcgctggatg gtgggacttt
     1921 tccacaaggg tcaattcatc cgtgctcagc aatcgctgtt catgcttgat gctgcgagcc
     1981 atgccctact cctccctgac actcttgcga tcgatcccac cgccctactc cgcgacaatt
     2041 cgaatcaatg taccatcaca ataatcgctc aatttctgcg attgtgtgat tggtgagatc
     2101 cttgcctttt tcggcgacga tcttgctttc cgtttccttc gaaaactcgc gccgcagatc
     2161 gccaagaatt tttgccgggg ccacgatgac aatttggcta aaggcggtgg acatcgcggc
     2221 cttattgatt tgttcggcga tcgacgcggc gaaacgctcc ttttgcagtt cgtgccagtc
     2281 cgtctcttcg acggagctgc gaatttggcc aacgctcgca taggcccgtc cgggcgcatc
     2341 acttccctgt tcgcgcgttg gtggatcggg gttttccaac actgacagac gccgcagatt
     2401 gggaaatttc tcgtccccct cattgatcag aaacagagcc ttttgaccat cgcccacgaa
     2461 gacccaggcg ccatgggata atttaaaatg catcaacgac tctctcc
//
LOCUS       DATMPU010000388         2499 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_1860147,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000388 DATMPU010000000
VERSION     DATMPU010000388.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 2499)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2499)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2499
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1860147"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..412)
                     /locus_tag="VNY06_08850"
     CDS             complement(<1..412)
                     /locus_tag="VNY06_08850"
                     /inference="COORDINATES: protein motif:HMM:NF039194.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5681 domain-containing protein"
                     /protein_id="HWX14932.1"
                     /translation="MPQFQKGQSGNPAGRPPGSRNRATSMVQNLLEGAAENIAKRAAQ
                     LAEEGNVAAIRICMNRLSPVGQHNPVAFELPPIHSTQDCLRATSAILTGIASGDIAPS
                     AAMQIARVIDVHLRAIATNDLEARMTRLEEDSANS"
     gene            complement(716..1525)
                     /locus_tag="VNY06_08855"
     CDS             complement(716..1525)
                     /locus_tag="VNY06_08855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX14933.1"
                     /translation="MTGAMTAIQAAITEAGRLEKVLKDGRPRVQVTLTDERATAKATA
                     LAWFGNHKQSILNVMRGADTAQIDDGYKKILVSAERAGARTKYLSVLRAIRKALIRLQ
                     SECATTPASATPTGDTAPNFAPLISDTAMQKILNARWHECTKCIGADAPLAGTVMMGG
                     LLEALLLARINREPDKKAIFTAKAAPKDRSSGKTKPLSEWTLKDYIDVVHELGWITVS
                     AKDVGAVLRDYRNYIHPQKQLSHSVHLKLDDARLFWEISKSIARQVIDSTK"
     gene            2089..>2499
                     /locus_tag="VNY06_08860"
     CDS             2089..>2499
                     /locus_tag="VNY06_08860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX14934.1"
                     /translation="MTYQKVWAKVIPLAFKAFQERRPNLRAPVTGPKLGRSWDVTPIS
                     LSPPACDATTSRVWRCTMSDKRKAFEAIVAKLEKLLPHLGDENTNEAGVALQKINSLL
                     HAAKLDWHDLVALMSEQKEGSVFSDLLRRLSEKDA"
BASE COUNT          459 a          722 c          705 g          613 t
ORIGIN      
        1 ccgaattggc gctgtcctcc tccagcctcg tcatgcgcgc ttccagatca ttcgtcgcaa
       61 tcgcgcggag gtgcacgtcg atcaccctgg cgatctgcat cgccgcggag ggtgcaatat
      121 ctccgctggc gatgccggtc aggatggcgg acgtggcgcg cagacaatcc tgcgtcgagt
      181 ggatgggcgg cagctcgaag gcaaccggat tatgctggcc gacgggcgag aggcggttca
      241 tgcaaatgcg gatggcggcc acgttgccct cctcggccag ctgggcggca cgcttggcaa
      301 tgttttccgc cgcgccctcg agcaggttct gcaccatgct cgtcgcgcgg ttgcgcgagc
      361 ccggcggccg gccggccgga tttccgcttt ggcctttctg aaattgcggc attctcctca
      421 ctcctctgct ggttttgggg tttggggtgc ggggagggag gcggcggcca gaaaacgctt
      481 ccgtttgggg tcccccggca ttggggcctc aaaaaaccca aggcgttttt cgggtgcctc
      541 gcgtccgcgc aatgaaaaac ccggcgcggt ttcccgggcc gggcttgggc acactcctga
      601 aggatatctt tttatacttg agtcccgttt caacgtccag aagtcagaag ttgcacaggg
      661 gtcacaagtg caccacgcag aggttgtatt cggcatccca agaaatattt gttcctcatt
      721 tcgtgctgtc gatgacctgc cgagctatgc tcttgctgat ttcccaaaac agcctcgcat
      781 catccagctt caaatgcaca ctgtgtgata gctgcttttg cggatgaatg tagttgcgat
      841 agtcgcgaag cacggcaccc acgtccttag ctgaaacggt gatccagcca agttcatgaa
      901 caacgtcgat gtagtctttg agcgtccatt ccgaaagcgg cttggttttg ccgctcgaac
      961 gatccttcgg tgctgccttg gccgtgaaga tcgctttctt atccggctcc cggttgatcc
     1021 gcgcaagcaa aagggcttct agcaagccgc ccatcatcac tgttcctgcg agtggcgcgt
     1081 ccgctccgat gcacttcgtg cactcgtgcc agcgtgcatt gagaattttc tgcatcgctg
     1141 tgtcagaaat cagaggcgcg aaattcggag cggtatcgcc ggttggcgtt gcgctcgctg
     1201 gcgtcgtggc gcattccgat tgcagccgta tcagtgcttt ccttattgcc cttaggacag
     1261 agagatattt cgtacgtgct ccggcacgct cagccgagac gaggattttc ttgtaaccgt
     1321 catcgatttg agcggtatca gcacctctca ttacattgag aatcgattgt ttatgattcc
     1381 caaaccacgc aagcgccgtc gccttagcgg tcgcgcgctc atctgtcagc gtcacttgga
     1441 cacgtgggcg accatctttc aacactttct caagccgacc cgcttctgtg atggccgcct
     1501 gtattgcggt catcgccccg gtcatggtcg cgtctttcgc cccctcgctt tttgtcgcgg
     1561 tttccgtcga gttgaaggta cgccgcttcc ggctttagcc caatcgctat attccctgtc
     1621 aactgcaccg ttcgtgttta acgcttgcca atcagccttt tcccaaagtc ggacgatttg
     1681 gaaatcttca ctggattgtc cgtcggggga tcgcgccgta atatctaccg ggtcattctg
     1741 ctgcctggat tccgatttcc atgacgaccg acccgtgaac ttgcaaagat tttctaccgc
     1801 ttgccattcc gtaagctctt ttttgtcctt ccagttcaat ctccgaacca caagttgctt
     1861 ggcattgctc tggggtccgg gaatcttcgt ggcctgtaga cttgctccaa ctgcggtcag
     1921 tgacgcaatc tccttacgtt gatcggcggc tgagcgttgg tcggcggctg acgttatggt
     1981 tctccaaact gtcgccaacc gctgtcgcca ctgtcgccag aatactatgt aggtcattga
     2041 tttattagct ggcgctccct agggaagcga gtgaagtaag cgaattcaat gacttatcaa
     2101 aaggtttggg ccaaggttat cccactggca ttcaaggcgt ttcaggaacg acgcccaaac
     2161 cttcgggcac cggtgacggg tccgaagctt ggccggtcat gggacgtcac gcctatctcg
     2221 ctctcgccgc cggcgtgcga cgcgaccacc tcccgcgttt ggaggtgcac gatgagtgac
     2281 aagcgcaagg cgtttgaagc gattgttgcg aagcttgaga agctgctccc gcatttaggg
     2341 gacgagaaca cgaacgaagc cggcgtcgct ctccagaaga tcaacagcct gctccacgcg
     2401 gcgaagctcg attggcatga tcttgtcgcg ctgatgtccg agcagaagga agggtcagtc
     2461 ttctctgatc tgctgcgacg gctctcggag aaagatgcg
//
LOCUS       DATMPU010000389         8012 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_4335328,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000389 DATMPU010000000
VERSION     DATMPU010000389.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 8012)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8012)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8012
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4335328"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..334)
                     /locus_tag="VNY06_08865"
     CDS             complement(<1..334)
                     /locus_tag="VNY06_08865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX14935.1"
                     /translation="MIGVPRRDHRLDTALCQVVANLIAVVALVAEKLVGTRVVKGPER
                     GEALRLVNLAARNIEGQRVAFGIRAEVDFGREAAARAAERFMCRPQTSARSSRTLRLS
                     AARSGASAP"
     gene            complement(868..1959)
                     /locus_tag="VNY06_08870"
     CDS             complement(868..1959)
                     /locus_tag="VNY06_08870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012591149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HWX14936.1"
                     /translation="MIWPPQQEAALNAVAEWLKRGEPQLFRLFGYAGTGKTTLAKKIA
                     EDVDGDVAFAAFTGKAALVMRSKGCEDARTIHSLIYRRCETDSGELSFVLNEDSAVAR
                     SRLVIIDECSMVDAELGRDLLSFGKPVLVLGDPGQLPPVKGGGFFTGATPDVMLTEVH
                     RQASDNPILRLSMLVREGKSLSLGSYGESQVIKRKEIDPGMVTVADQLLVGLNRTRRS
                     YNQRMRELFERTSAFPEAGDKIVCLKNNNAKGLLNGGIWLVKSTAKTRKKKLLMNVIS
                     EDDLAAKPLRIGVLPEFFQGLEETLPPLVRRDSDEFDFGYALTVHKAQGSQWDQVILF
                     DESFAFREHRDRWLYSGITRASKQLTIVV"
     gene            complement(2040..2564)
                     /gene="folK"
                     /locus_tag="VNY06_08875"
     CDS             complement(2040..2564)
                     /gene="folK"
                     /locus_tag="VNY06_08875"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018266610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HWX14937.1"
                     /translation="MPSLAEQLRGGEPLTEAGLALGSNVGDKPANIRRALALLEERGI
                     LRLTAVSSIYRTPPWGYEEQEFFANACALGETRLPPLELLAAVNTIEKDMGRRPSVRW
                     GPRLIDIDILFYGTGYFEDTKLSLPHKSMFDRAFVLVPLAEIAPSLEISGHSVAKAAE
                     NFAAEQIEKWEADA"
     gene            complement(2548..3375)
                     /gene="folP"
                     /locus_tag="VNY06_08880"
     CDS             complement(2548..3375)
                     /gene="folP"
                     /locus_tag="VNY06_08880"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003613579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HWX14938.1"
                     /translation="MIAPFMTARRDRFLGLIGKKPVLMGILNVTPDSFSDGGKFFAPE
                     AAMEHGRKLAAEGADIIDIGAESTRPGHTPVTAEQELARLEPVLGKLTSSVDVPFSID
                     TTKARIARRAAELGVSVVNDVWGFQKDPAMADTVAETGMAAVIMHNRHAIDPGLDIIA
                     DMRHFFDRSLHLAHAAAVPRARLILDPGIGFGKNRAQNYSALANLGALKDYGLPILIG
                     VSRKSLLAETGSMPDNLNLIRTVAANLAAATNGASIFRVHDPVEHVAAFKVFDAVAR"
     gene            complement(3473..4282)
                     /locus_tag="VNY06_08885"
     CDS             complement(3473..4282)
                     /locus_tag="VNY06_08885"
                     /EC_number="1.2.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012591402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018493 - formylmethanofuran dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formylmethanofuran dehydrogenase subunit C"
                     /protein_id="HWX14939.1"
                     /translation="MKSLVLTLKHEPNQRLDLSPLVPHSLSGKSAKDIAVIELQTTRE
                     KLTAGDIFKMRTGGVESIRFEGGSSRLDNIGLQMKAGEIIVEGDCGKSLGRGMSGGNI
                     VAYRDCGPFAGSAMSGGRLEIAGDAGNFLGAPLEGEMEGMTGGLLIVRGNAGERAGDR
                     LRRGTMVIEGSAGDFPGSRMLAGTLIVLGGCGRLPGYLMRRGTIVLAESPELATTFVN
                     CGEHELAFAGVFSNLLRPESRAAARLFGRPLMRFAGDMAALGKGEIFFPGW"
     gene            complement(4279..5187)
                     /gene="fhcD"
                     /locus_tag="VNY06_08890"
     CDS             complement(4279..5187)
                     /gene="fhcD"
                     /locus_tag="VNY06_08890"
                     /EC_number="2.3.1.101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020174429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylmethanofuran--tetrahydromethanopterin
                     N-formyltransferase"
                     /protein_id="HWX14940.1"
                     /translation="MRPIKKNGVRIDDTFAEAFPMRGTAILITAPNRRWAMQAATTMT
                     GFATSVIGCGAEAGIDCERPKDSTPDGRPGVRVLTFSVSTDTLQSQLVNRLGQCVLTS
                     PGSACFADLDGADRLQLGDAIRYFGDGWQISKKFGDKHFWRVPVMDGEFLCEGTTGLT
                     KNAVGGGNLLIMGTTAKTTMRAAELAVEAMNGVEDVVLPFPGGIARSGSKVGSKYKAM
                     TASTNDAYCPTLRGAVATQLDEDVGCVLEIVIDGLTEQAVAEAMRAGITAIVKLGPEQ
                     GAVRVGAGNYGGKLGPFHFHLKDLLA"
     gene            complement(5175..6809)
                     /locus_tag="VNY06_08895"
     CDS             complement(5175..6809)
                     /locus_tag="VNY06_08895"
                     /EC_number="1.2.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016919509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formylmethanofuran dehydrogenase subunit A"
                     /protein_id="HWX14941.1"
                     /translation="MLIHLKGGRVVDPVNGCDEVADLWIENDRIVGAPEGKQADDVHD
                     VSGKIVMAGAIDIHSHIAGGNVNSARLLLPEKHQNFLPHPIALPFSTAKWSTFEVGTI
                     YASMGFTTVIEPAIVPHQAFHAHLELADIPIIDKGILAVLGNDDFLLSLLRHGESSSA
                     VSDYVASAVAASNGLGVKCINAGGATAFKYNVRTFSLDDVVPFYGLTSRNIVEALQGA
                     VENLKIPHPLHLHANNLGIPGNVATALATIQAAQGERLHLAHLQFYSYGSEGNHGFSS
                     AAAELGAAVNAAPNVTVDVGQVMFRQTVTISADVLRQFNGAAMANPRKSIIHDGDDNG
                     GGIVPYLYRENNFANAVQWACGLELFLLIADPWRVFFTTDHPNGAPFTTYPEIFALIM
                     SRDARAEAIARLPQEAVAMTTLPSIAREYTFGEIATMTRAAPAKLLGLKDRGHLGEGA
                     LADIAVYSPGSDLAKMFRFAHLVFKDGDLVVRDGQVTHYRWGKALKVNPGYDKAIDRR
                     LSDYYDRHFGNSRDLFTVREHLLPHAEPFREVACAQ"
     gene            complement(6810..7898)
                     /locus_tag="VNY06_08900"
     CDS             complement(6810..7898)
                     /locus_tag="VNY06_08900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012591399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tungsten formylmethanofuran dehydrogenase"
                     /protein_id="HWX14942.1"
                     /translation="MSAARIDGKPVSLGEAAQRAAEILSHAQFPVITGLGTDVAGARA
                     SIHLASKLRGAYDHMRSAQIFADLDVMRQSGMMYTTPSEARLRADVFLFIGKDLTKVW
                     PRMMEHLSPAEIPPLDLAPEKRKLLWIAPGRALKAEGLTIDTLDSSNLHMTLAGVRAR
                     VAGKPVSCTKSVRGKLDQFAEILKHARFGVAVWSSDSLDSLSIEMLHGLLRDLNQKTR
                     FSGLPLGSGANASGVVQTSGWMTGFPVRTSFGRGFPEHDTWRFNASRMIETGEADAAL
                     WISAYDAEPLQWKRNVPLVALVSPQTAFAREPRVYIEVGCPGRDHDAAEFERETNSIV
                     ARTASRPSPAPSVEMVLKQISAHLGENA"
BASE COUNT         1735 a         2298 c         2339 g         1640 t
ORIGIN      
        1 acggcgcgct ggcgccactg cgggcggccg aaagccgcag cgttctcgac gagcgcgcgc
       61 tggtctgcgg tcggcacatg aagcgctcgg ccgctcgcgc ggcggcttct cgaccaaaat
      121 ccacctccgc acgaatgcca aaggcgaccc gctgaccttc gatattacgg gcggcgaggt
      181 tcacgagacg caaggcttcg ccgcgctcag ggcccttcac gacgcgcgtc ccgacaagct
      241 tctcggcgac aagggctacg acagcgatga gattcgcaac gacctggcaa agcgcggtat
      301 cgagccggtg atcccgccgc ggaacgccaa tcaaatacga tcgcgaagcc tacaagcggc
      361 gcaatctgat cgagcggtgc gtcaaccgtc aagcagttcc gccgcatcgc gacccgttac
      421 gaaaaaactg ccagagctta cctttcaatg ctatgcattg cagccgcaag gctctggata
      481 aaaactgtca acacggccta gtccacaatt gaagaaccag gacggcagag ccaatattta
      541 ccccagaggt aacgccaact ccattttgcc aagttaactg accattattt acagccgcga
      601 tcttttttac gcagcgagca tcttgacaga ttgtcgccag tgccgacact ggtccggtca
      661 taggcgatcc acttttccaa atcggagtta ccgttgcggt tgcgcaacat tttcttaaaa
      721 gccattgatt tgcgtcctca aattgatggt taggggcggg ccgttacgag tgccttgcct
      781 gccctgctaa tatgtgacgg ctgagacaaa ttgtccagca tcatgccgca agaatcgaca
      841 tctccgtttg gggaaaccct gcccagatca gaccacaatt gttagctgtt ttgaggcacg
      901 cgtaatgccg ctatagagcc aacggtcgcg gtgttcgcga aaggcaaagc tttcatcaaa
      961 caggattacc tggtcccatt gcgatccttg ggccttatgt acagtaagcg cgtagccgaa
     1021 gtcgaattca tccgagtctc gccggacgag aggcggaaga gtttcctcca atccctggaa
     1081 aaactccggc agaacgccaa ttcggagcgg cttagctgcg agatcgtcct cagaaatcac
     1141 attcatcaaa agttttttct tgcgggtctt ggcggtgctt ttgacaagcc agataccgcc
     1201 gttcaaaagc cctttcgcat tgttgttttt gagacagacg attttgtcgc cggcctcggg
     1261 aaacgcgctg gtgcgctcaa aaagctcgcg cattctttgg ttatacgagc ggcgcgtgcg
     1321 gttgaggcca acgagaagct gatcggcgac cgttaccatg ccgggatcga tctctttgcg
     1381 cttgatgacc tggctttcgc cataagagcc gagactgagg ctttttccct cgcggacgag
     1441 catcgacaat cggagaatgg gattgtccga tgcttgccgg tgcacctcgg tcagcatcac
     1501 atccggggtt gctccggtga aaaatccgcc gcccttgacg ggaggcagct ggcctggatc
     1561 gcccaggacc aaaacgggct tgccaaagga caaaagatcg cggccaagtt ccgcgtcgac
     1621 catggaacat tcgtcgatga tgacaagcct cgatctcgca acggcactat cttcgttaag
     1681 aacgaaagag agctcaccgg aatccgtttc gcaacgccgg tagatcaaac tgtggattgt
     1741 gcgtgcatcc tcgcagccct tggaccgcat gacaagagcg gcctttccag tgaaggctgc
     1801 gaaagcgaca tcgccgtcaa cgtcttcggc gatcttcttg gcgagcgtgg ttttgcccgt
     1861 gccggcatag ccaaaaagcc ggaacaattg cggctccccg cgctttagcc attcggcgac
     1921 ggcgtttaag gccgcttctt gctgcggcgg ccaaatcata tgctgtgggc aggcttgccg
     1981 aggcgcgagg taagcaacgg gaaagcgacg gcaaatgccg acatggaacg gccaaaagat
     2041 cacgcgtcgg cctcccattt ttcaatctgc tcggccgcga aattttccgc agccttggcg
     2101 acggaatgcc ctgaaatttc taaagatgga gcgatctcgg ccaacggcac gagaacaaaa
     2161 gcacggtcaa acatagattt atgtggcaaa cttaatttgg tatcttcaaa atagcctgtt
     2221 ccatagaaca agatgtcaat atctatgagg cgcggccccc agcggacgga gggccgccgg
     2281 cccatgtcct tctcgattgt attgacggcg gcaaggagtt ccaacggggg caggcgggtc
     2341 tctcccaatg cgcaggcatt ggcaaaaaac tcctgctcct catagcccca gggtggcgtg
     2401 cggtagatcg acgaaacagc tgtcagcctt agaattccgc gttcttcgag cagcgcgagc
     2461 gcacgcctaa tatttgctgg cttgtcaccg acattgctcc cgagagcaag tcctgcctcg
     2521 gtcaatggct cgccgccccg cagttgctca gcgagcgacg gcatcgaaaa ccttgaaagc
     2581 ggcgacatgc tcgaccggat cgtgaacccg gaatattgag gcgccgttgg tggcggctgc
     2641 aagattggcg gcgaccgtac gaatgagatt gagattatct ggcattgagc cggtttccgc
     2701 gagcagcgat ttgcgcgata cgccaatcag gattggcagg ccataatcct ttagcgcccc
     2761 gagattggcc aatgcggagt aattttgcgc cctgttcttg ccgaagccga tgccggggtc
     2821 gaggatgaga cgcgcgcggg gaacagctgc ggcatgggca aggtgcaagg atcggtcgaa
     2881 gaaatggcgc atatccgcga tgatgtcgag gccagggtcg attgcgtggc gattgtgcat
     2941 aataaccgcg gccataccgg tttcggcaac cgtatcggcc atggcggggt ctttttggaa
     3001 tccccaaaca tcattaacga cgcttacgcc gagttctgcc gcgcggcggg caatacgggc
     3061 cttggtggta tcgatcgaaa acggaacgtc gacggaactc gtcagcttcc caaggaccgg
     3121 ttccaggcgg gcgagctcct gttctgccgt gacgggagta tgccctgggc gggtcgattc
     3181 ggctccgata tcgataatgt ctgccccttc ggcggcgagt ttcctcccat gttccatagc
     3241 agcttcgggg gcaaaaaatt ttccgccatc ggagaaggag tccggcgtca cattgagtat
     3301 gcccatgagg accggctttt tcccaataag accgagaaac cggtcgcggc gggcggtcat
     3361 aaagggcgcg atcattgtaa ggtcccgatt tgccaggtga agcccgattc ggacttggcg
     3421 aggcaaagcg aataattgcg caggatcggg ggaggggcct tttgaagtgc gattaccatc
     3481 ctgggaaaaa gatttctccc ttgccgagcg ccgccatatc tccggcaaaa cgcatgagcg
     3541 ggcggccaaa gagccttgcg gcagcccggc tttcgggacg caaaaggttt gagaagaccc
     3601 cagcgaaggc cagttcatgc tcgccgcaat tgacaaaggt tgtggcgagt tctggcgact
     3661 cggcaaggac gattgtgccc cggcgcatca gataacctgg caaacggccg cagccgccaa
     3721 gcacgatgag ggtgccagca agcatgcggc tgccgggaaa atctccggcg gagccttcga
     3781 tcaccattgt tccgcggcgc aggcggtcgc ccgcgcgctc gcccgcgttt ccgcggacaa
     3841 tcagcaggcc cccggtcatg ccttccatct cgccttcgag cggcgcgcct aaaaagtttc
     3901 cggcatcacc agcaatctcc agccgtcctc ccgacatcgc ggaccccgca aaagggccgc
     3961 aatcgcgata ggccacgata ttgccgccgc tcatgccgcg gccgagcgat ttgccgcaat
     4021 cgccttcgac aatgatttct cccgccttca tttgaagacc gatattgtcg aggcgggatg
     4081 atcccccttc aaaccttatc gactccacgc cgccagtccg cattttgaat atatctccgg
     4141 cggtgagttt ctcgcgggta gtctgcagtt cgattacagc gatatccttg gccgatttgc
     4201 cactgagcga atgcgggacg agcggagaaa gatccagtct ttgatttggc tcgtgcttga
     4261 gagtgaggac aagcgatttc acgccaggag atccttcaaa tgaaaatgaa acggaccgag
     4321 cttcccgccg taattgccgg ccccaacgcg cacggcgccc tgctcgggtc cgagcttcac
     4381 aattgccgta atcccagccc gcattgcctc ggcgacggct tgttccgtca ggccgtcgat
     4441 gacgatttcg aggacgcagc cgacatcctc gtcgagctgt gtcgccaccg cgccgcggag
     4501 cgttgggcaa taggcgtcat tggtcgacgc ggtcatggcc ttgtattttg aaccaacctt
     4561 cgagcccgac cgggcaatac cgccagggaa aggcaaaacg acgtcctcga caccattcat
     4621 cgcctcaacg gcgagttccg cggcgcgcat tgtcgtcttg gcggtcgtgc ccataatgag
     4681 caggttgccg ccgccgaccg catttttcgt gaggcctgtt gtcccctcgc agaggaattc
     4741 gccgtccatc accgggacac gccaaaaatg tttgtccccg aatttttttg aaatctgcca
     4801 gccgtcgccg aaatagcgga tggcatcgcc aagctgtaat ctgtcggcgc cgtcgaggtc
     4861 ggcgaaacag gcagagcccg gcgatgtcaa cacgcattgg cccaagcggt tgacgagctg
     4921 ggactgcaaa gtgtcggtcg atacggaaaa cgttagcacg cggacgccag ggcgcccgtc
     4981 tggcgtgctg tccttcggcc tttcgcaatc gattcccgct tcggcgccgc agccgatcac
     5041 ggaggtggca aaaccggtca ttgtcgttgc cgcctgcatg gcccagcggc ggtttggcgc
     5101 ggtgatgaga atcgccgtgc cacgcattgg aaaggcctcg gcgaaagtat catcaatgcg
     5161 gacgccgttt ttttttattg ggcgcatgcg acctcccgga aaggttcggc atgcggaagg
     5221 agatgctctc tgaccgtgaa caaatcgcga gaattgccga aatggcggtc atagtagtcc
     5281 gaaaggcgcc ggtcgatggc cttgtcatag cccggattga ccttgagcgc tttgccccag
     5341 cgatagtggg tgacttgccc gtcgcgcaca accaaatcgc catccttaaa caccaaatgg
     5401 gcgaagcgga acattttggc gaggtcggag cccggggaat agacggcgat gtctgccaac
     5461 gccccttcgc caaggtggcc ccggtcctta agaccaagca actttgccgg ggcagcgcgg
     5521 gtcatggtgg cgatctctcc aaaagtgtat tcgcgcgcga tggatggtag cgtcgtcatt
     5581 gcaacggctt cctgcggcag gcgggcgatc gcttccgccc gcgcatcgcg actcatgatc
     5641 agcgcgaaaa tttccggata ggtggtgaaa ggcgcgccat ttgggtggtc ggtcgtgaag
     5701 aaaacccgcc aaggatccgc gatcagcaag aacaattcaa gtccgcaggc ccattgcacg
     5761 gcattggcga aattattctc cctatacaaa taaggaacga tgccgccgcc attgtcgtcg
     5821 ccatcatgga ttattgattt tcttgggttt gccattgcag cgccattgaa ctgccgcagc
     5881 acgtcggccg aaatcgtgac cgtttgccgg aacatgacct ggccgacatc gacggtcaca
     5941 ttgggcgctg cattcacggc agcgccaagt tcggccgcgg ccgatgaaaa gccatggttg
     6001 ccttcgctgc cataggaata gaattggaga tgcgccaaat gcaagcgctc gccctgcgct
     6061 gcttggatcg ttgcaagcgc ggtcgcgaca ttccccggaa tgccgaggtt attggcgtgc
     6121 agatgcagcg gatgcggaat tttaaggttc tcgacggcgc cctgcagggc ctcgacgata
     6181 ttgcgtgagg tgagcccgta gaagggtacc acatcatcga ggctgaacgt ccgaacattg
     6241 tatttgaacg cagttgcgcc accggcatta atgcatttga ccccaaggcc attgcttgcc
     6301 gcgacggcgg atgcgacgta atcagacacc gcagacgaac tctctccgtg gcgcaaaagg
     6361 gacaataaaa aatcgtcatt tccgaggacg gcaagaattc ctttgtcgat gatgggaatg
     6421 tcggcgagtt cgagatgggc gtgaaacgcc tgatgcggca cgatggcagg ctcgattacc
     6481 gtggtgaagc ccatggacgc gtagatggtg ccgacctcaa aggttgacca tttggccgtg
     6541 gagaaaggca gcgcgatcgg gtgcggcaag aagttttggt gcttttcggg aagcagcagg
     6601 cgggccgaat ttacgttgcc gccagcgata tgggaatgaa tatcgatggc gccagccatg
     6661 acaatcttgc cggagacgtc atgcacgtca tcggcctgtt tgccctcggg cgctccgacg
     6721 atgcggtcat tttcgatcca aagatcggcg acttcgtcgc atccattgac aggatcgacg
     6781 acccggcccc ccttcaggtg aatgagcatt taggcgttct ccccaaggtg cgccgagatt
     6841 tgcttaagta ccatctcaac ggatggcgct gggcttggcc gcgacgctgt gcgcgcgaca
     6901 atcgaattgg tctcgcgttc gaattcggct gcatcatgat ctcgtcccgg acagccaacc
     6961 tcgatgtaga cgcgcggctc gcgcgcgaat gccgtttgag gtgagacgag cgcgacaagg
     7021 ggcacatttc gcttccattg gagaggctca gcatcataag cggaaatcca caaagcggca
     7081 tcagcctcac cggtttcgat catgcgcgag gcattaaacc gccaggtgtc atgctccggg
     7141 aagccacggc caaatgacgt gcgcaccgga aagccggtca tccagcccga ggtttgtacg
     7201 acgccggatg cgttagcgcc gcttccgagc ggcagccctg agaaacgcgt tttctggttg
     7261 agatcgcgga gaagtccatg cagcatttca atcgaaagac tatcgagaga gtcgctgctc
     7321 cagacagcaa cgccaaaccg cgcatgcttt agaatttccg cgaattggtc gagcttgccc
     7381 cgaacgcttt tcgtgcagct tactggcttg cctgcgacac gggctcgcac gcccgccagc
     7441 gtcatgtgca gattggaaga atcaagagta tcgatggtca gcccttcggc cttcaaagcg
     7501 cgtccgggcg caatccataa cagcttgcgc ttttcgggcg caagatcaag cggcgggatc
     7561 tcagcgggcg acaggtgctc catcattctc ggccagactt tcgtcaaatc cttgccgatg
     7621 aacaggaaaa cgtctgcccg caggcgggct tcgcttggtg tcgtatacat catgcctgat
     7681 tgacgcatga cgtcaaggtc tgcgaaaatt tgcgcagagc gcatatggtc ataggcgccc
     7741 cgaagctttg aggcgagatg aatggacgcg cgagccccag cgacatccgt gccaaggccg
     7801 gtaatcaccg ggaattgcgc gtgcgacaga atttccgccg cgcgttgggc cgcttcgcca
     7861 agcgaaacag gcttgccgtc aatcctcgcc gcgctcatat tttctccccg tggtttcgat
     7921 acccctcggt ttatgaggca tatcgctgcc agcatcaata tggctcacgg ctacgtcatg
     7981 ctttacgagg ttgcaaggcg gaaccgaatt tt
//
LOCUS       DATMPU010000390         2291 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methylocella sp. isolate SMAG_U12910 k141_4335353,
            whole genome shotgun sequence.
ACCESSION   DATMPU010000390 DATMPU010000000
VERSION     DATMPU010000390.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943517
            Sequence Read Archive: SRR7101595
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylocella sp. (soil metagenome)
  ORGANISM  Methylocella sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Beijerinckiaceae; Methylocella.
REFERENCE   1  (bases 1 to 2291)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2291)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:44:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,785
            CDSs (total)                      :: 1,771
            Genes (coding)                    :: 1,688
            CDSs (with protein)               :: 1,688
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 50 of 83
            Pseudo Genes (incomplete)         :: 47 of 83
            Pseudo Genes (internal stop)      :: 6 of 83
            Pseudo Genes (multiple problems)  :: 18 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2291
                     /organism="Methylocella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4335353"
                     /isolate="SMAG_U12910"
                     /isolation_source="soil"
                     /db_xref="taxon:1978226"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            502..858
                     /locus_tag="VNY06_08905"
     CDS             502..858
                     /locus_tag="VNY06_08905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="Rap1a/Tai family immunity protein"
                     /protein_id="HWX14943.1"
                     /translation="MRALLTLCFCVFSIAAHAEVDLKRFLENYDGAAIAEKQHLAERL
                     LDMQYGMAWTNAVIEAETGTHFYCPPQTSPLTGEQLVDMLRKNAAQDPRLNNISFGLA
                     ILSTLRKTFPCGEKPN"
     gene            973..1200
                     /locus_tag="VNY06_08910"
     CDS             973..1200
                     /locus_tag="VNY06_08910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014492640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX14944.1"
                     /translation="MPERRFAPPWTVEETDACFIVRDATGQALAYVYFEDEPGRRAAA
                     KLLTRDEARRIAANIAKLPWAKIDGAPNGSR"
     gene            1283..1453
                     /locus_tag="VNY06_08915"
     CDS             1283..1453
                     /locus_tag="VNY06_08915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX14945.1"
                     /translation="MTGRACLNTKHCIAILALAAGLGLLVAMWAAPPPLAFTDRWVPL
                     LKRDEAALFVGE"
     gene            complement(1586..2035)
                     /locus_tag="VNY06_08920"
     CDS             complement(1586..2035)
                     /locus_tag="VNY06_08920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX14946.1"
                     /translation="MSTDKPAVDVDLAKRQRRVADLAASVNARITSAGGGDDGGGGED
                     THMGGGSHKMATESELRAAEIAAAEARTDTKITRMEGKLDLVISKLDGVREDNRSTRA
                     NQWVIGFGLAVLIVAVAALFPVFFDMGSKIHEIIEKAVQSRVINTPK"
     gene            complement(2025..>2291)
                     /locus_tag="VNY06_08925"
     CDS             complement(2025..>2291)
                     /locus_tag="VNY06_08925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWX14947.1"
                     /translation="PLLARLGRGVEVNPEQALIDLLRQLSRSHFALMHAVFALEANVH
                     LISISFEAGVAPTDADKKKWQEIRDQFQKHMQAVLLWRNQRHEY"
BASE COUNT          493 a          683 c          629 g          486 t
ORIGIN      
        1 tggaccgccg gaatttcaag gtgccacccg tcgcgcgcat tccgcgtctt caaagcccac
       61 ttagcgcgag gtaggcgtca caaaccccgg gcttgtttgc atttcaccct gttcatgcgc
      121 ggcaacgtcg accagtcgaa gccgaagtcg cattcaggag cgccacgcga acagatccga
      181 tattcgccca ggcttggtac aatcttggcg acttgttaga cgaccagggt cgcgccgacg
      241 cggccatcga atgcttacgc aaagcgctgc gggcggcgcc tgattacgca gatgcaatgt
      301 tcaacctcgc cctactgcta caacgaaaaa accaataccc cgaggccgcc gagtactggc
      361 gccgctatct caccagcgat tgccagtcgg aatgggcgat gcgagcccgc cggtcattga
      421 agttttgtga gatgcagaga cacttgagcg cctctgcttg aggttctcga atccacgcta
      481 atgaatttta ggagccaatc gatgcgcgcg ttgctcacac tttgtttttg cgtgttttca
      541 atcgccgcac atgcagaagt cgatttaaaa cgctttcttg aaaactacga tggtgctgct
      601 attgccgaaa agcaacattt ggccgaacgc cttttggata tgcaatacgg aatggcgtgg
      661 acaaatgcgg taatcgaggc agagacagga acgcatttct actgcccacc tcagacatcg
      721 cctctcactg gcgaacaact agtagatatg ctccggaaaa acgcagcaca ggacccgcgc
      781 ctcaacaata tatcgtttgg tttggcaatt ctcagtacat tgcgcaaaac ctttccgtgc
      841 ggtgaaaagc caaactgagg cacgtcatgg ccgcggaagc gccgatttga ttgcgtgcgc
      901 tgagccgttc ggtagcacgc gcctttgccg agttcgagcc cagcgtgccc gccgcgcgtg
      961 ctctaatggg ccatgcccga gcgccgcttc gcgccgccct ggaccgtcga ggagaccgat
     1021 gcctgcttca tcgtccgcga cgccaccggg caggcgcttg cctacgtcta tttcgaggac
     1081 gagcccggac gccgcgcggc ggccaagctg ctcactcgtg acgaagcccg gcgcatcgcg
     1141 gcgaacatcg caaagctgcc gtgggcgaaa atcgacggcg ctccgaacgg ttcgagatga
     1201 caaaggccag agagcggctg acaagaacga ggcacgaggt cgagcgtgct atggtgtgca
     1261 tggcttatgg agcgcagcgg tcgtgaccgg ccgggcatgc ctgaatacga aacactgtat
     1321 cgccatcctc gccctggcgg caggtctcgg cttgctcgtc gccatgtggg ctgcgccccc
     1381 gccactcgct tttacggatc gctgggtgcc gctgctcaag cgtgacgaag ccgccttgtt
     1441 cgtgggtgaa tgactgccgg gcggggctga tccacagacc ggagagttag atggcgcaga
     1501 acgcgggcca cctaacgtta cagtacggtt gcggagtttc agttccgcta caacaaccga
     1561 atgaatgacg atattttcgg agcggctatt tcggtgtgtt aatgacgcga gactgcactg
     1621 ccttctcaat tatttcgtgg atcttgcttc ccatatcgaa aaacaccgga aagagtgcgg
     1681 caaccgcaac gatgagaacc gccaaaccga agcctattac ccactgattg gcgcgcgtgg
     1741 aacggttgtc ttcgcgaaca ccatctagct tcgagatcac gagatcgagc ttgccctcca
     1801 tgcgggtgat ctttgtgtcc gttcgcgctt ctgcggcggc tatctcagcg gcccttaatt
     1861 cactctcggt tgccatttta tgactaccac ctcccatgtg ggtatcttca ccgccgccgc
     1921 catcatcacc gccacccgca gaagtgatcc tggcgttaac agaggctgct agatcggcta
     1981 ctcggcgctg acgcttagca aggtcaacgt ccacggcagg cttgtcagta ctcatgacgt
     2041 tggttccgcc atagcaagac cgcttgcatg tgtttctgaa attggtccct gatctcctgc
     2101 cactttttct tgtctgcatc cgtcggagcc acgccagcct caaaacttat agaaataaga
     2161 tgtacgttcg cctccaaagc aaagaccgcg tgcattaacg caaagtgcga cctgctcagt
     2221 tggcgcagca gatcaattaa cgcttgctct ggattcactt caacacccct ccccagacgc
     2281 gccaggaggg g
//
